title: Electropolymerization of taurine on gold surface and its sensory application for determination of captopril in undiluted human serum.
abstract: Polytaurine film was electrodeposited on gold (Au) electrode through cyclic voltammetry from taurine and phosphate buffer solution. The electrocatalytic effect of polytaurine modified Au (PT/Au) electrode was investigated for electro-oxidation of captopril (CAP). Electrocatalytical activity of PT/Au electrode was studied using cyclic voltammetry (CV), chronoamperometry and differential pulse voltammetry (DPV). DPV was used to evaluate the analytical performance of CAP in the presence of phosphate buffer solution and good limit of detection was obtained by this sensor. The experimental conditions influencing the determination of CAP were optimized and under optimal conditions, the oxidation peak current was proportional to CAP concentration in the range of 0.06-0.2 μM, while the detection limit was 0.03 μM (S/N=3). The results revealed that PT promotes the rate of oxidation by increasing the peak current. Finally, the applicability of the method to direct assay of human serum is described. The proposed sensor was successfully applied to determine cadaverine in fish samples, yielding satisfactory results. The spiked recoveries were in the range of 96.0-105.0%.
authors.affiliation: Drug Applied Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz 51664, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Hasanzadeh
authors.affiliation: Electroanalytical Chemistry Laboratory, Faculty of Chemistry, University of Tabriz, Tabriz, Iran.
authors.forename: Mohammad Hossein
authors.initials: MH
authors.lastname: Pournaghi-Azar
authors.affiliation: Biochemistry Department, Genetic and Metabolism Group, Pasteur Institute of Iran, P.O. Box 13164 Tehran, Iran.
authors.forename: Nasrin
authors.initials: N
authors.lastname: Shadjou
authors.affiliation: Drug Applied Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz 51664, Iran. Electronic address: ajouyban@hotmail.com.
authors.forename: Abolghasem
authors.initials: A
authors.lastname: Jouyban
mesh_terms: D002216:Captopril / Q000097:blood*; D004560:Electricity; D004563:Electrochemistry / Q000379:methods; D004566:Electrodes; D006046:Gold / Q000737:chemistry*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D010084:Oxidation-Reduction; D058105:Polymerization; D013499:Surface Properties; D013654:Taurine / Q000737:chemistry*
chemical_list: D013654:Taurine; D006046:Gold; D002216:Captopril
keywords: Biomedical; Captopril; Electropolymerization; Human serum; Nanosensor; Polytaurine