title: [Methods for the Crimean-Congo hemorrhage fever diagnosis].
abstract: Several distinct methods currently used for the Crimean-Congo Hemorrhage Fever diagnosis (CCHF) were suggested in this work. We demonstrated that the ELISA-based diagnostic kits, which are based on CCHFV recombinant antigens produced in E. coli cells, still possessed a few substantial shortcomings, which are yet to be addressed. In this work we presented the development of the unique CCHFV detection system fully based on reverse transcription--nested two-step polymerase chain reaction (RT-PCR). Our RT-PCR-based diagnostic kit for the CCHFV detection is now commercially available. We also developed a simple screening method for the samples, potentially containing CCHFV, which is based on restriction fragment length polymorphism (RFLP) amplicons analysis and allows for preliminary genotyping of the CCHFV isolates.
authors.forename: S V
authors.initials: SV
authors.lastname: Seregin
authors.forename: V S
authors.initials: VS
authors.lastname: Petrov
authors.forename: M P
authors.initials: MP
authors.lastname: Grishaev
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D004926:Escherichia coli; D005838:Genotype; D003225:Hemorrhagic Fever Virus, Crimean-Congo / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D006479:Hemorrhagic Fever, Crimean / Q000175:diagnosis* / Q000473:pathology; D006801:Humans; D019590:Nucleocapsid Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D019590:Nucleocapsid Proteins