title: Investigation of deletion of 22pb in KIR2DS4 gene in a population of southern Brazil.
abstract: BACKGROUND
  The aim of this study was to investigate the distribution of full-length and deleted variants of KIR2DS4 in a population of southern Brazil and compare the results with other populations, as well as comparing two techniques, PCR-SSP and PCR-SSO, for typing of variants.
  
  
  METHODS
  258 individuals from southern Brazil were analysed by PCR-SSO ("polymerase chain reaction-sequence specific oligonucleotides", One Lambda, Inc., Canoga Park, CA), of which 161 were also analysed by PCR-SSP.
  
  
  RESULTS
  The study population showed similarities with other Caucasian populations; 46.5% of individuals had only KIR2DS4 variants, 21.3% had the full-length form and 25.1% had both forms.
  
  
  CONCLUSIONS
  The frequencies found in both groups (genotyped by PCR-SSP and PCR-SSO) were 100% concordant.
authors.affiliation: Departamento de Ciências Básicas da Saúde, Laboratório de Imunogenética, Universidade Estadual de Maringá, Av. Colombo 5790, Maringá, PR 87020-900, Brazil.
authors.forename: Amanda Vansan
authors.initials: AV
authors.lastname: Marangon
authors.forename: Jeane Eliete Laguila
authors.initials: JE
authors.lastname: Visentainer
authors.forename: Gláucia Andréia Soares
authors.initials: GA
authors.lastname: Guelsin
authors.forename: Samaia Laface
authors.initials: SL
authors.lastname: Clementino
authors.forename: Cristiane Conceição Chagas
authors.initials: CC
authors.lastname: Rudnick
authors.forename: Fabiano Cavalcante
authors.initials: FC
authors.lastname: de Melo
authors.forename: Marco Antonio
authors.initials: MA
authors.lastname: Braga
authors.forename: Ana Maria
authors.initials: AM
authors.lastname: Sell
mesh_terms: D001938:Brazil; D005838:Genotype; D006801:Humans; D059645:Mutation Rate; D016133:Polymerase Chain Reaction / Q000379:methods; D054340:Receptors, KIR / Q000737:chemistry / Q000235:genetics*; D017384:Sequence Deletion
chemical_list: C473953:KIR2DS4 protein, human; D054340:Receptors, KIR
keywords: KIR; KIR2DS4; KIR2DS4-deleted variant; genotyping