title: Reducing blood glucose levels in TIDM mice with an orally administered extract of sericin from hIGF-I-transgenic silkworm cocoons.
abstract: In previous studies, we reported that the blood glucose levels of mice with type I diabetes mellitus (TIDM) was reduced with orally administered silk gland powder from silkworms transgenic for human insulin-like growth factor-I (hIGF-I). However, potential safety hazards could not be eliminated because the transgenic silk gland powder contained heterologous DNA, including the green fluorescent protein (gfp) and neomycin resistance (neo) genes. These shortcomings might be overcome if the recombinant hIGF-I were secreted into the sericin layer of the cocoon. In this study, silkworm eggs were transfected with a novel piggyBac transposon vector, pigA3GFP-serHS-hIGF-I-neo, containing the neo, gfp, and hIGF-I genes controlled by the sericin-1 (ser-1) promoter with the signal peptide DNA sequence of the fibrin heavy chain (Fib-H) and a helper plasmid containing the piggyBac transposase sequence under the control of the Bombyx mori actin 3 (A3) promoter, using sperm-mediated gene transfer to generate the transformed silkworms. The hIGF-I content estimated by enzyme-linked immunosorbent assay was approximately 162.7 ng/g. To estimate the biological activity of the expressed hIGF-I, streptozotocin-induced TIDM mice were orally administered sericin from the transgenic silkworm. The blood glucose levels of the mice were significantly reduced, suggesting that the extract from the transgenic hIGF-I silkworm cocoons can be used as an orally administered drug.
authors.affiliation: School of Biology & Basic Medical Science, Soochow University, Suzhou 215123, PR China.
authors.forename: Zuowei
authors.initials: Z
authors.lastname: Song
authors.affiliation: School of Biology & Basic Medical Science, Soochow University, Suzhou 215123, PR China.
authors.forename: Mengyao
authors.initials: M
authors.lastname: Zhang
authors.affiliation: School of Biology & Basic Medical Science, Soochow University, Suzhou 215123, PR China; National Engineering Laboratory for Modern Silk, Soochow University, Suzhou, PR China.
authors.forename: Renyu
authors.initials: R
authors.lastname: Xue
authors.affiliation: School of Biology & Basic Medical Science, Soochow University, Suzhou 215123, PR China; National Engineering Laboratory for Modern Silk, Soochow University, Suzhou, PR China.
authors.forename: Guangli
authors.initials: G
authors.lastname: Cao
authors.affiliation: School of Biology & Basic Medical Science, Soochow University, Suzhou 215123, PR China; National Engineering Laboratory for Modern Silk, Soochow University, Suzhou, PR China. Electronic address: gongcl@suda.edu.cn.
authors.forename: Chengliang
authors.initials: C
authors.lastname: Gong
mesh_terms: D000284:Administration, Oral; D000595:Amino Acid Sequence; D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D001786:Blood Glucose / Q000032:analysis*; D012831:Bombyx / Q000235:genetics*; D017931:DNA Primers; D007334:Insulin-Like Growth Factor I / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D047030:Sericins / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D001786:Blood Glucose; D017931:DNA Primers; D047030:Sericins; D007334:Insulin-Like Growth Factor I
keywords: Diabetic mice; Insulin-like growth factor; Transgenic silkworm cocoons