title: Antioxidant activity of bovine casein hydrolysates produced by Ficus carica L.-derived proteinase.
abstract: A Ficus carica L. latex proteinase preparation was investigated for its ability to produce antioxidant hydrolysates/peptides from bovine casein (CN). The Oxygen Radical Absorbance Capacity (ORAC) values for NaCN and β-CN hydrolysates ranged from 0.06 to 0.18, and from 0.51 to 1.19μmol Trolox equivalents/mg freeze-dried sample, respectively. Gel permeation HPLC showed that the β-CN hydrolysate with a degree of hydrolysis of 21% had 65% of peptide material with a molecular mass <500Da. The RP-UPLC profiles also indicated that β-CN was substantially hydrolysed during the early stages of hydrolysis. Analysis of the 4h β-CN hydrolysate by LC-ESI-MS/MS allowed identification of 8 peptide sequences with potential antioxidant properties.
authors.affiliation: Department of Life Sciences, University of Limerick, Limerick, Ireland; Department of Agronomy, Food, Natural Resources, Animals and Environment - DAFNAE, Università di Padova, Legnaro, Padova, Italy.
authors.forename: Giovanna
authors.initials: G
authors.lastname: Di Pierro
authors.affiliation: Department of Life Sciences, University of Limerick, Limerick, Ireland.
authors.forename: Martina B
authors.initials: MB
authors.lastname: O'Keeffe
authors.affiliation: Department of Life Sciences, University of Limerick, Limerick, Ireland; Food for Health Ireland, Department of Life Sciences, University of Limerick, Limerick, Ireland.
authors.forename: Alexey
authors.initials: A
authors.lastname: Poyarkov
authors.affiliation: Department of Agronomy, Food, Natural Resources, Animals and Environment - DAFNAE, Università di Padova, Legnaro, Padova, Italy.
authors.forename: Giovanna
authors.initials: G
authors.lastname: Lomolino
authors.affiliation: Department of Life Sciences, University of Limerick, Limerick, Ireland; Food for Health Ireland, Department of Life Sciences, University of Limerick, Limerick, Ireland. Electronic address: dick.fitzgerald@ul.ie.
authors.forename: Richard J
authors.initials: RJ
authors.lastname: FitzGerald
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry; D002364:Caseins / Q000737:chemistry*; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods*; D030681:Ficus / Q000737:chemistry*; D010084:Oxidation-Reduction; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000975:Antioxidants; D002364:Caseins; C475729:casein hydrolysate
keywords: Antioxidant activity; Bioactive peptides; Degree of hydrolysis; Ficus carica L.; Mass spectrometry; ORAC(FL) assay; Sodium caseinate; β-Casein