title: Point-of-care fluorescence immunoassay for cardiac panel biomarkers.
abstract: BACKGROUND
  Myoglobin, creatine kinase-MB isoenzyme (CK-MB), and cardiac troponin I (cTnI) are cardiac biomarkers that are widely used to assist in the early and late diagnoses of acute myocardial infarction (AMI). Here, we present a clinically applicable fluorescence (FL) immunoassay for cardiac biomarkers.
  
  
  METHODS
  Whole blood was mixed with FL-labeled detector Ab (antibody) and then loaded onto a capture Ab-immobilized strip in a test cartridge. The FL intensities at test and control line on the strip were obtained and converted in a laser FL scanner to determine the concentration of biomarker. The analytical performance of immunoassay system was evaluated by linearity and imprecision tests. The comparability of the FL immunoassay method was examined with a reference method.
  
  
  RESULTS
  FL intensities and the levels of myoglobin, CK-MB, and cTnI displayed good linearity and high correlations (r = 0.999, 0.998, and 0.989, respectively). The coefficient of variations (CVs) of imprecision for all cardiac biomarkers were less than 8% in both intra- and interassays. When the results from the developed method and bioMerieux VIDAS assay were analyzed by Bland-Altman plot and Passing-Bablok plot, the two assay methods were in good agreement.
  
  
  CONCLUSIONS
  The FL immunoassay system can provide a platform for point-of-care testing (POCT), and it is an easy, fast, and reliable method for the quantification of cardiac biomarkers.
authors.affiliation: Department of Biomedical Science, Hallym University, Chuncheon, South Korea; BodiTech Med, Inc, Chuncheon, South Korea.
authors.forename: Tae Kyum
authors.initials: TK
authors.lastname: Kim
authors.forename: Sang Wook
authors.initials: SW
authors.lastname: Oh
authors.forename: Soon Cheol
authors.initials: SC
authors.lastname: Hong
authors.forename: Young Joon
authors.initials: YJ
authors.lastname: Mok
authors.forename: Eui Yul
authors.initials: EY
authors.lastname: Choi
mesh_terms: D015415:Biomarkers / Q000097:blood; D052279:Creatine Kinase, MB Form / Q000097:blood; D005455:Fluorescent Antibody Technique; D015200:Fluoroimmunoassay / Q000379:methods; D006801:Humans; D009203:Myocardial Infarction / Q000175:diagnosis*; D009211:Myoglobin / Q000097:blood; D019095:Point-of-Care Systems; D012680:Sensitivity and Specificity; D019210:Troponin I / Q000097:blood
chemical_list: D015415:Biomarkers; D009211:Myoglobin; D019210:Troponin I; D052279:Creatine Kinase, MB Form
keywords: AMI; CK-MB; POCT; cTnI; fluorescence immunochromatographic assay; myoglobin