title: Water-soluble polysaccharides from finger citron fruits (Citrus medica L. var. sarcodactylis).
abstract: Four water-soluble polysaccharides, FCp-1, FCp-2, FCp-3, and FCp-4 were obtained from finger citron fruits (Citrus medica L. var. sarcodactylis) by hot-water extraction and ethanol precipitation, followed by routine separation procedure. Based on the calibration curve, molecular weights of them were estimated to be 113.9, 32.6, 140.3, and 177.1 kDa respectively. The acid hydrolysis, methylation, IR, GC-MS, and NMR experiments were used for composition analysis. FCp-1 was a heteropolysaccharide composed of arabinose, galactose, glucose, rhamnose, and xylose, with a molar ratio of 3.0:7.0:4.1:1.0:1.5. FCp-2 and FCp-4 were →4)-α-D-GalpA(1→ linking galacturonan differ in molecular weights. FCp-3 was a →6)-α-D-Glcp(1→ linking glucan. According to the results of in vitro assays, FCp-3 showed significantly and moderately enhancing capacities toward the proliferation of splenocytes and thymocytes respectively. Thus, FCp-3 or analogs may have further use as immunomodulatory agents.
authors.affiliation: Department of Chemistry, Zhejiang University, Hangzhou 310027, People's Republic of China; Department of Pharmacy, Dali College, Dali 671000, People's Republic of China.
authors.forename: Zhengchun
authors.initials: Z
authors.lastname: He
authors.affiliation: Department of Chemistry, Zhejiang University, Hangzhou 310027, People's Republic of China.
authors.forename: Fengjie
authors.initials: F
authors.lastname: Liang
authors.affiliation: Department of Chemistry, Zhejiang University, Hangzhou 310027, People's Republic of China.
authors.forename: Yuyang
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Chemistry, Zhejiang University, Hangzhou 310027, People's Republic of China. Electronic address: cheyjpan@zju.edu.cn.
authors.forename: Yuanjiang
authors.initials: Y
authors.lastname: Pan
mesh_terms: D001089:Arabinose / Q000032:analysis; D049109:Cell Proliferation / Q000187:drug effects; D002957:Citrus / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry*; D005690:Galactose / Q000032:analysis; D005947:Glucose / Q000032:analysis; D006868:Hydrolysis; D008970:Molecular Weight; D010936:Plant Extracts / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D012210:Rhamnose / Q000032:analysis; D012995:Solubility; D013154:Spleen / Q000166:cytology / Q000187:drug effects; D060168:Thymocytes / Q000166:cytology / Q000187:drug effects; D014867:Water; D014994:Xylose / Q000032:analysis
chemical_list: D010936:Plant Extracts; D011134:Polysaccharides; D014867:Water; D014994:Xylose; D001089:Arabinose; D005947:Glucose; D012210:Rhamnose; D005690:Galactose
keywords: Citrus medica L. var. sarcodactylis Hort; Immunological activity; Polysaccharide