title: Cell-scaffold interaction within engineered tissue.
abstract: The structure of a tissue engineering scaffold plays an important role in modulating tissue growth. A novel gelatin-chitosan (Gel-Cs) scaffold with a unique structure produced by three-dimensional printing (3DP) technology combining with vacuum freeze-drying has been developed for tissue-engineering applications. The scaffold composed of overall construction, micro-pore, surface morphology, and effective mechanical property. Such a structure meets the essential design criteria of an ideal engineered scaffold. The favorable cell-matrix interaction supports the active biocompatibility of the structure. The structure is capable of supporting cell attachment and proliferation. Cells seeded into this structure tend to maintain phenotypic shape and secreted large amounts of extracellular matrix (ECM) and the cell growth decreased the mechanical properties of scaffold. This novel biodegradable scaffold has potential applications for tissue engineering based upon its unique structure, which acts to support cell growth.
authors.affiliation: Rapid Manufacturing Engineering Center, Mechatronic Engineering and Automation of Shanghai University, Shanghai 200444, PR China.
authors.forename: Haiping
authors.initials: H
authors.lastname: Chen
authors.affiliation: Rapid Manufacturing Engineering Center, Mechatronic Engineering and Automation of Shanghai University, Shanghai 200444, PR China. Electronic address: Yuanyuan_liu@shu.edu.cn.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Rapid Manufacturing Engineering Center, Mechatronic Engineering and Automation of Shanghai University, Shanghai 200444, PR China.
authors.forename: Zhenglong
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: Rapid Manufacturing Engineering Center, Mechatronic Engineering and Automation of Shanghai University, Shanghai 200444, PR China.
authors.forename: Weihua
authors.initials: W
authors.lastname: Chen
authors.affiliation: Rapid Manufacturing Engineering Center, Mechatronic Engineering and Automation of Shanghai University, Shanghai 200444, PR China.
authors.forename: Yongzhe
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Rapid Manufacturing Engineering Center, Mechatronic Engineering and Automation of Shanghai University, Shanghai 200444, PR China.
authors.forename: Qingxi
authors.initials: Q
authors.lastname: Hu
mesh_terms: D017667:Adipocytes / Q000187:drug effects* / Q000502:physiology; D053687:Adult Stem Cells / Q000187:drug effects* / Q000502:physiology; D002448:Cell Adhesion; D049109:Cell Proliferation; D048271:Chitosan / Q000494:pharmacology; D005780:Gelatin / Q000494:pharmacology; D006801:Humans; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D005780:Gelatin; D048271:Chitosan
keywords: Cell compatibility; Cell–scaffold interaction; Mechanical property; Porous; Surface morphology