title: Molecular analysis of enterovirus in Cameroon by partial 5'UTR-VP4 gene sequencing reveals a high genetic diversity and frequency of infections.
abstract: Enteroviruses (EVs) often infect humans, presenting as endemic or epidemic infections. In this study, the diversity of infecting EVs was studied among 146 children and 137 adults in a small community in Cameroon. The participants provided 2,458 fecal samples during 1-year monthly collection; 10 or more samples were obtained from 55%. Partial 5'UTR-VP4 region could be sequenced in 393/547 PCR positive samples obtained from 119 children and 85 adults. EV-RNA was detected in at least one sample from 235 participants (83%) during the study period. A total of 121 different strains were identified, 66 infected only children, 29 only adults, and 26 infected both children and adults. There were children with up to five episodes with different strains, and adults with up to four such episodes. Infants aged <5 years were significantly more often EV infected compared to older participants. Infections with species EV-C constituted two third of all cases, and overall EV infections were more common during the rainy season. Species EV-B more often infected children than adults. Most strains were detected only for certain months of the year; however five strains were observed during the time spans of 5-10 months. Two strains were excreted up to eight months in three children and one adult. In 11 of the 128 families with paired samples the child and the adult were infected simultaneously by the same strain, indicating common source of infection. The study revealed a surprising complexity of EV ecosystem in a single community.
authors.affiliation: Department of Infectious Diseases/Section of Clinical Virology, Institute of Biomedicine, University of Gothenburg, Gothenburg, Sweden.
authors.forename: James Ayukepi
authors.initials: JA
authors.lastname: Ayukekbong
authors.forename: Cajetan
authors.initials: C
authors.lastname: Fobisong
authors.forename: Magnus
authors.initials: M
authors.lastname: Lindh
authors.forename: Theresia
authors.initials: T
authors.lastname: Nkuo-Akenji
authors.forename: Tomas
authors.initials: T
authors.lastname: Bergström
authors.forename: Helene
authors.initials: H
authors.lastname: Norder
mesh_terms: D020121:5' Untranslated Regions; D000293:Adolescent; D000328:Adult; D017677:Age Distribution; D000368:Aged; D002163:Cameroon / Q000453:epidemiology; D002648:Child; D002675:Child, Preschool; D016000:Cluster Analysis; D004770:Enterovirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D004769:Enterovirus Infections / Q000453:epidemiology* / Q000821:virology*; D005243:Feces / Q000821:virology; D005260:Female; D014644:Genetic Variation; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D012008:Recurrence; D012621:Seasons; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D015678:Viral Structural Proteins / Q000235:genetics*; D017201:Virus Shedding; D055815:Young Adult
chemical_list: D020121:5' Untranslated Regions; D015678:Viral Structural Proteins
keywords: Cameroon; human enterovirus; molecular epidemiology; shedding; species