title: Evidence for decreased expression of ADAMTS-1 associated with impaired oocyte quality in PCOS patients.
abstract: BACKGROUND
  Polycystic ovary syndrome (PCOS) is the most common cause of dysfunctional ovulation-affecting female fertility. A disintegrin and metalloproteinase with thrombospondin-like motifs (ADAMTS-1) is required for normal ovulation and subsequent fertilization, and the expression of ADAMTS-1 may be altered in PCOS granulosa cell (GC)-reflecting abnormalities in ovulatory signaling.
  
  
  OBJECTIVE
  The purpose of this paper is to analyze the differential expression of ADAMTS-1 in PCOS patients associated with impaired oocyte quality.
  
  
  METHODS
  A prospective comparative experimental study was performed at a clinical reproductive medicine center.
  
  
  METHODS
  Women with PCOS (n = 40) and normovulatory controls (n = 40) undergoing controlled ovarian hyperstimulation and in vitro fertilization were recruited in our study.
  
  
  METHODS
  Differential expression of ADAMTS-1 in GCs was analyzed with immunocytochemistry in PCOS patients and normal controls, and ADAMTS-1 mRNA expression was quantified by RT-PCR. Furthermore, the correlation between ADAMTS-1 mRNA and oocyte quality was analyzed.
  
  
  RESULTS
  The expression of ADAMTS-1 was decreased in PCOS patients compared with normally ovulating women and was closely related to lower oocyte recovery, oocyte maturity, and fertilization rate.
  
  
  CONCLUSIONS
  Our study provides evidence that the dysregulated expression of ADAMTS-1 in PCOS may influence oocyte quality-via GCs-oocyte paracrine and endocrine mechanism.
authors.affiliation: Reproductive Medicine Center, First Affiliated Hospital of Sun Yat-Sen University, Guangdong, People's Republic of China 510080.
authors.forename: Shan
authors.initials: S
authors.lastname: Xiao
authors.forename: Yubin
authors.initials: Y
authors.lastname: Li
authors.forename: Tao
authors.initials: T
authors.lastname: Li
authors.forename: Minghui
authors.initials: M
authors.lastname: Chen
authors.forename: Yanwen
authors.initials: Y
authors.lastname: Xu
authors.forename: Yangxing
authors.initials: Y
authors.lastname: Wen
authors.forename: Canquan
authors.initials: C
authors.lastname: Zhou
mesh_terms: D051722:ADAM Proteins / Q000235:genetics* / Q000378:metabolism; D000071097:ADAMTS1 Protein; D000328:Adult; D002478:Cells, Cultured; D015536:Down-Regulation; D005260:Female; D005307:Fertilization in Vitro; D006107:Granulosa Cells / Q000378:metabolism; D056945:Hep G2 Cells; D006801:Humans; D054315:Oocyte Retrieval; D009865:Oocytes / Q000378:metabolism / Q000473:pathology*; D010062:Ovulation Induction; D011085:Polycystic Ovary Syndrome / Q000235:genetics* / Q000473:pathology*; D011247:Pregnancy; D011786:Quality Control
chemical_list: D051722:ADAM Proteins; D000071097:ADAMTS1 Protein; C103859:ADAMTS1 protein, human