title: Mass spectrometric screening of ovarian cancer with serum glycans.
abstract: Changes of glycosylation pattern in serum proteins have been linked to various diseases including cancer, suggesting possible development of novel biomarkers based on the glycomic analysis. In this study, N-linked glycans from human serum were quantitatively profiled by matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) and compared between healthy controls and ovarian cancer patients. A training set consisting of 40 healthy controls and 40 ovarian cancer cases demonstrated an inverse correlation between P value of ANOVA and area under the curve (AUC) of each candidate biomarker peak from MALDI-TOF MS, providing standards for the classification. A multibiomarker panel composed of 15 MALDI-TOF MS peaks resulted in AUC of 0.89, 80~90% sensitivity, and 70~83% specificity in the training set. The performance of the biomarker panel was validated in a separate blind test set composed of 23 healthy controls and 37 ovarian cancer patients, leading to 81~84% sensitivity and 83% specificity with cut-off values determined by the training set. Sensitivity of CA-125, the most widely used ovarian cancer marker, was 74% in the training set and 78% in the test set, respectively. These results indicate that MALDI-TOF MS-mediated serum N-glycan analysis could provide critical information for the screening of ovarian cancer.
authors.affiliation: ASTA Inc., Gyeonggi Biocenter, Eui-dong, Youngtong-gu, Suwon 443-270, Republic of Korea ; Department of Food and Nutrition, Chungnam National University, Yuseong-gu, Daejeon 305-764, Republic of Korea.
authors.forename: Jae-Han
authors.initials: JH
authors.lastname: Kim
authors.affiliation: ASTA Inc., Gyeonggi Biocenter, Eui-dong, Youngtong-gu, Suwon 443-270, Republic of Korea.
authors.forename: Chang Won
authors.initials: CW
authors.lastname: Park
authors.affiliation: NosQuest Inc., U-Space 1A-dong 1103, Bundang-gu, Seongnam 463-400, Republic of Korea.
authors.forename: Dalho
authors.initials: D
authors.lastname: Um
authors.affiliation: NosQuest Inc., U-Space 1A-dong 1103, Bundang-gu, Seongnam 463-400, Republic of Korea.
authors.forename: Ki Hwang
authors.initials: KH
authors.lastname: Baek
authors.affiliation: Hudson Surface Technology Inc., 180 Old Tappan Road, Building 1, Suite 3, Old Tappan, NJ 07675, USA.
authors.forename: Yohahn
authors.initials: Y
authors.lastname: Jo
authors.affiliation: ASTA Inc., Gyeonggi Biocenter, Eui-dong, Youngtong-gu, Suwon 443-270, Republic of Korea ; Graduate School of Analytical Science and Technology, Chungnam National University, Yuseong-gu, Daejeon 305-764, Republic of Korea.
authors.forename: Hyunjoo
authors.initials: H
authors.lastname: An
authors.affiliation: ASTA Inc., Gyeonggi Biocenter, Eui-dong, Youngtong-gu, Suwon 443-270, Republic of Korea.
authors.forename: Yangsun
authors.initials: Y
authors.lastname: Kim
authors.affiliation: Department of Obstetrics and Gynecology, Cheil General Hospital and Women's Healthcare Center, Kwandong University, College of Medicine, Mukjeong-dong, Jung-gu, Seoul 100-380, Republic of Korea.
authors.forename: Tae Jin
authors.initials: TJ
authors.lastname: Kim
mesh_terms: D000328:Adult; D019540:Area Under Curve; D018394:CA-125 Antigen / Q000097:blood*; D016022:Case-Control Studies; D055088:Early Detection of Cancer; D005260:Female; D006801:Humans; D008403:Mass Screening; D008875:Middle Aged; D018297:Neoplasms, Cystic, Mucinous, and Serous / Q000097:blood / Q000175:diagnosis*; D010051:Ovarian Neoplasms / Q000097:blood / Q000175:diagnosis*; D011134:Polysaccharides / Q000097:blood; D012372:ROC Curve; D016037:Single-Blind Method; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D055815:Young Adult
chemical_list: D018394:CA-125 Antigen; D011134:Polysaccharides