title: Expression profiles of NOS isoforms in gingiva of nNOS knockout mice.
abstract: Nitric oxide is a gaseous molecule associated with many distinct physiological functions, and is derived from L-arginine catalyzed by nitric oxide synthase (NOS). Nitric oxide synthase has 3 isoforms: nNOS, iNOS and eNOS. Although these NOS isoforms are believed to play an important role in gingival tissue, little information is available on their morphological dynamics. The aim of this study was to investigate the profiles of NOS isoforms in deficiency of nNOS in gingiva of mice. Twelve male (6 normal (C57BL/6) and 6 nNOS knockout) mice were used. All mice were 5-week-old, weighing approximately 20-25 g each. After sacrifice, the jaws of the mice were removed by mechanical means and specimens analyzed by histology, in situ hybridization and immunohistochemistry. Immunohistochemical observation revealed positive staining for iNOS and eNOS, especially in lamina propria. Similar results in the mRNA expression levels were shown by in situ hybridization analysis. It may suggest that iNOS and eNOS compensated nNOS deficiency in the gingiva of nNOS knockout mice.
authors.affiliation: Department of Pediatric Dentistry, Tokyo Dental College, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Ishioka
authors.affiliation: Department of Epidemiology and Public Health, Tokyo Dental College, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Ishizuka
authors.affiliation: Department of Pediatric Dentistry, Tokyo Dental College, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Shintani
authors.affiliation: Department of Ultrastructural Science, Tokyo Dental College, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Yanagisawa
authors.affiliation: Department of Clinical Pathophysiology, Tokyo Dental College, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Inoue
authors.affiliation: School of Nursing, Niimi College, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Sasaki
authors.affiliation: Oral Histology, Department of Morphological Biology, Ohu University School of Dentistry, Japan. Electronic address: hi-watanabe@den.ohu-u.ac.jp.
authors.forename: H
authors.initials: H
authors.lastname: Watanabe
mesh_terms: D000818:Animals; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D005881:Gingiva / Q000166:cytology / Q000201:enzymology*; D007527:Isoenzymes / Q000096:biosynthesis / Q000235:genetics; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D052248:Nitric Oxide Synthase Type I / Q000096:biosynthesis* / Q000235:genetics; D052247:Nitric Oxide Synthase Type II / Q000096:biosynthesis* / Q000235:genetics; D052250:Nitric Oxide Synthase Type III / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D007527:Isoenzymes; D052248:Nitric Oxide Synthase Type I; D052247:Nitric Oxide Synthase Type II; D052250:Nitric Oxide Synthase Type III; C496321:Nos1 protein, mouse; C496317:Nos2 protein, mouse; C496315:Nos3 protein, mouse
keywords: Gingiva; Isoforms; Knockout mice; Lamina propria; Nitric oxide synthase