title: Stress-dependent regulation of 13-lipoxygenases and 13-hydroperoxide lyase in olive fruit mesocarp.
abstract: The effect of different environmental stresses on the expression and enzyme activity levels of 13-lipoxygenases (13-LOX) and 13-hydroperoxide lyase (13-HPL) and on the volatile compounds synthesized by their sequential action has been studied in the mesocarp tissue of olive fruit from the Picual and Arbequina cultivars. The results showed that temperature, light, wounding and water regime regulate olive 13-LOXs and 13-HPL genes at transcriptional level. Low temperature and wounding brought about an increase in LOX and HPL enzyme activities. A very slight increase in the total content of six straight-chain carbons (C6) volatile compounds was also observed in the case of low temperature and wounding treatments. The physiological roles of 13-LOXs and 13-HPL in the olive fruit stress response are discussed.
authors.affiliation: Department of Biochemistry and Molecular Biology of Plant Products, Instituto de la Grasa (CSIC), 41012 Sevilla, Spain. Electronic address: npadilla@ujaen.es.
authors.forename: María N
authors.initials: MN
authors.lastname: Padilla
authors.affiliation: Department of Biochemistry and Molecular Biology of Plant Products, Instituto de la Grasa (CSIC), 41012 Sevilla, Spain. Electronic address: mlhdez@cica.es.
authors.forename: M Luisa
authors.initials: ML
authors.lastname: Hernández
authors.affiliation: Department of Biochemistry and Molecular Biology of Plant Products, Instituto de la Grasa (CSIC), 41012 Sevilla, Spain. Electronic address: carlos.sanz@ig.csic.es.
authors.forename: Carlos
authors.initials: C
authors.lastname: Sanz
authors.affiliation: Department of Biochemistry and Molecular Biology of Plant Products, Instituto de la Grasa (CSIC), 41012 Sevilla, Spain. Electronic address: mrivas@cica.es.
authors.forename: José M
authors.initials: JM
authors.lastname: Martínez-Rivas
mesh_terms: D000446:Aldehyde-Lyases / Q000235:genetics / Q000378:metabolism*; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism*; D004789:Enzyme Activation; D005638:Fruit / Q000201:enzymology* / Q000378:metabolism; D008084:Lipoxygenase / Q000235:genetics / Q000378:metabolism*; D031658:Olea / Q000201:enzymology* / Q000378:metabolism; D013312:Stress, Physiological
chemical_list: D003577:Cytochrome P-450 Enzyme System; C117433:13-lipoxygenase; D008084:Lipoxygenase; D000446:Aldehyde-Lyases; C054540:hydroperoxide lyase
keywords: Abiotic stress; Enzyme activity; Gene expression; Hydroperoxide lyase; Lipoxygenase; Olea europaea; Oleae; Olive; Volatile compounds