title: [Correlation of the t(9;22), t(12;21), and DNA hyperdiploid content with immunophenotype and proliferative rate of leukemic B-cells of pediatric patients with B-cell acute lymphoblastic leukemia].
abstract: BACKGROUND
  Between 60 and 80% of patients with B-cell acute lymphoblastic leukemia show genetic abnormalities which influence the prognosis of the disease and the biology of the tumor.
  
  
  OBJECTIVE
  To analyze different genetic abnormalities in acute B lymphoblastic leukemia in children, its relationship with the immunophenotype and the proliferative rate compared with normal B cell precursors.
  
  
  METHODS
  We assessed immunophenotype, DNA content and proliferative rate in 44 samples by flow cytometry, and translocations t(9;22), t(12;21), t(4;11), and t(1;19) by RT-PCR. Using a hierarchical cluster analysis, we identified some immunophenotypic patterns associated to genetic abnormalities when compared with normal B cell precursors.
  
  
  RESULTS
  DNA quantification showed that 21% of the cases had high hyperdiploidy and 47.7% has low hyperdiploidy. The presence of hyperdiploidy was associated with increased tumor proliferation and aberrant immunophenotypes, including abnormal expression of CD10, TdT, CD38, and CD45 and an increased size of the lymphoblasts. The presence of t(9;22) and t(12;21) discriminates normal cells from tumor cells with aberrant immunophenotype in the expression of CD19, CD22, CD13, CD33, CD38, CD34, and CD45.
  
  
  CONCLUSIONS
  The aberrant immunophenotype profile detected in neoplastic cells along with abnormalities in the proliferative rate were significantly associated with DNA hyperdiploidy and clearly distinguished lymphoblasts with t(9;22) and t(12;21) from normal B cell precursors. The identification of these parameters is useful as a tool for classification and monitoring of these patients.
authors.affiliation: Departamento de Microbiología, Pontificia Universidad Javeriana, Bogotá, D.C, Colombia.
authors.forename: Sandra Milena
authors.initials: SM
authors.lastname: Quijano
authors.affiliation: Laboratorio de Genética Humana, Departamento de Ciencias Biológicas, Universidad de los Andes, Bogotá, D.C, Colombia.
authors.forename: María Mercedes
authors.initials: MM
authors.lastname: Torres
authors.affiliation: Departamento de Patología, Fundación Santa Fe de Bogotá, Bogotá, D.C, Colombia.
authors.forename: Liliana Edith
authors.initials: LE
authors.lastname: Vásquez
authors.affiliation: Departamento de Patología, Fundación Santa Fe de Bogotá, Bogotá, D.C, Colombia.
authors.forename: Gina Elizabeth
authors.initials: GE
authors.lastname: Cuéllar
authors.affiliation: Laboratoire de Pathologie, Université Paris Diderot, Paris, Francia.
authors.forename: Martha Liliana
authors.initials: ML
authors.lastname: Romero
authors.affiliation: Departamento de Patología, Fundación Santa Fe de Bogotá, Bogotá, D.C, Colombia.
authors.forename: Edna Liliana
authors.initials: EL
authors.lastname: Martín
authors.affiliation: Facultad de Medicina, Departamento de Pediatría/Oncohematología Pediátrica, Universidad Nacional de Colombia, Bogotá, D.C, Colombia.
authors.forename: Adriana
authors.initials: A
authors.lastname: Linares
authors.affiliation: Servicio de Onco-Hematología Pediátrica, Fundación Hospital de La Misericordia, Bogotá, D.C, Colombia.
authors.forename: Silverio
authors.initials: S
authors.lastname: Castaño
authors.affiliation: Servicio de Onco-Hematología Pediátrica, Fundación Hospital de La Misericordia, Bogotá, D.C, Colombia.
authors.forename: Isabel Cristina
authors.initials: IC
authors.lastname: Sarmiento
authors.affiliation: Servicio de Onco-Hematología Pediátrica, Fundación Hospital de La Misericordia, Bogotá, D.C, Colombia.
authors.forename: Edgar
authors.initials: E
authors.lastname: Cabrera
authors.affiliation: Servicio de Onco-Hematología Pediátrica, Fundación Hospital de La Misericordia, Bogotá, D.C, Colombia.
authors.forename: Gloria Inés
authors.initials: GI
authors.lastname: Uribe
authors.affiliation: Departamento de Patología, Fundación Santa Fe de Bogotá, Bogotá, D.C, Colombia.
authors.forename: Rafael Enrique
authors.initials: RE
authors.lastname: Andrade
authors.affiliation: Departamento de Patología, Fundación Santa Fe de Bogotá, Bogotá, D.C, Colombia.
authors.forename: Carlos Eugenio
authors.initials: CE
authors.lastname: Saavedra
mesh_terms: D000293:Adolescent; D001402:B-Lymphocytes / Q000145:classification*; D049109:Cell Proliferation; D002648:Child; D002675:Child, Preschool; D004273:DNA, Neoplasm / Q000032:analysis; D004171:Diploidy; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D007223:Infant; D015448:Leukemia, B-Cell / Q000235:genetics* / Q000276:immunology*; D008297:Male; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics* / Q000276:immunology*
chemical_list: D004273:DNA, Neoplasm