title: A novel aggregation-induced emission fluorescent probe for nucleic acid detection and its applications in cell imaging.
abstract: A new kind of aggregation-induced emission compound was synthesized and used as the probe of nucleic acid. The characterization of this compound was studied. Both the RNA and DNA were detected by using this probe. And the detection scope of DNA and RNA was different. We researched the selectivity of our probe in double and single strand DNA sequences. The visualization of gel electrophoresis and the cell nucleus imaging were researched as well. Compared with the traditional nucleus dye Hoechst 33258, our probe also has the potential to be nucleus dye. And the cell toxicity was well performed by MTT assays.
authors.affiliation: College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Hubei, Wuhan 430072, PR China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Xu
authors.affiliation: College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Hubei, Wuhan 430072, PR China.
authors.forename: Shengyong
authors.initials: S
authors.lastname: Yan
authors.affiliation: College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Hubei, Wuhan 430072, PR China.
authors.forename: Yimin
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Hubei, Wuhan 430072, PR China.
authors.forename: Rong
authors.initials: R
authors.lastname: Huang
authors.affiliation: College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Hubei, Wuhan 430072, PR China.
authors.forename: Yuqi
authors.initials: Y
authors.lastname: Chen
authors.affiliation: College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Hubei, Wuhan 430072, PR China.
authors.forename: Jiaqi
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Hubei, Wuhan 430072, PR China.
authors.forename: Xiaocheng
authors.initials: X
authors.lastname: Weng
authors.affiliation: College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Hubei, Wuhan 430072, PR China. Electronic address: xzhou@whu.edu.cn.
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhou
mesh_terms: D002467:Cell Nucleus / Q000187:drug effects; D004247:DNA / Q000032:analysis*; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D057054:Molecular Imaging / Q000379:methods*; D015394:Molecular Structure; D012313:RNA / Q000032:analysis*; D013050:Spectrometry, Fluorescence
chemical_list: D005456:Fluorescent Dyes; D012313:RNA; D004247:DNA
keywords: Aggregation-induced emission; Cell imaging; Dye; Fluorescent; Gel electrophoresis; Nucleic acid