title: Hypoglycaemic and tissue-protective effects of the aqueous extract of persea americana seeds on alloxan-induced albino rats.
abstract: BACKGROUND
  The tissue-protective potential of Persea americana necessitated a look into the histopathological effects of the plant extract on the pancreas, liver, and kidneys. This study was conceived and designed based on the gaps in the research that has been performed and what is known about the plant. The hypoglycaemic and tissue-protective effects of hot aqueous Persea americana (avocado pear) seed extracts on alloxan-induced albino rats were investigated.
  
  
  METHODS
  Persea americana seeds were extracted using hot water, and different concentrations of the extract were prepared. The effects of different concentrations (20, 30, 40 g/L) of the hot aqueous P. americana seed extract on alloxan-induced Wistar albino rats were compared with those of a reference drug, glibenclamide. The glucose level of the rats was measured daily, and the weight of the animal was monitored on a weekly basis for 21 days. The oral glucose tolerance test (OGTT) was performed at 0, 30, 60, 90 and 120 minutes, and the histopathologies of the liver, kidneys, and pancreas were investigated. Phytochemical analysis of P. americana seed extracts indicated the presence of glycosides, tannins, saponins, carbohydrates, flavonoids, and alkaloids.
  
  
  RESULTS
  The results showed that the extract possessed a significant hypoglycaemic (P < 0.05) effect and reversed the histopathological damage that occurred in alloxan-induced diabetic rats, comparable to the effects glibenclamide. The seeds of P. americana also had anti-diabetic and protective effects on some rat tissues such as the pancreas, kidneys, and liver.
  
  
  CONCLUSIONS
  In conclusion, the present study provides a pharmacological basis for the folkloric use of the hot-water extract of P. americana seeds in the management of diabetes mellitus.
authors.affiliation: Faculty of Pharmaceutical Sciences University of Port Harcourt, Port Harcourt, Rivers Sate PMB 5323, Nigeria.
authors.forename: Anthonet Ndidi
authors.initials: AN
authors.lastname: Ezejiofor
authors.affiliation: Faculty of Pharmaceutical Sciences University of Port Harcourt, Port Harcourt, Rivers Sate PMB 5323, Nigeria.
authors.forename: Abednego
authors.initials: A
authors.lastname: Okorie
authors.affiliation: Faculty of Pharmaceutical Sciences University of Port Harcourt, Port Harcourt, Rivers Sate PMB 5323, Nigeria.
authors.forename: Orish Ebere
authors.initials: OE
authors.lastname: Orisakwe
keywords: alloxan; hot aqueous; hypoglyceamic effect; persea americana; protective effect