title: Differential microRNA expression by Solexa sequencing in the sera of ovarian cancer patients.
abstract: MicroRNAs are a class of small noncoding RNA which play important regulatory roles in a variety of cancers. MiRNA-specific expression profiles have been reported for several pathological conditions. In this study, we combined large scale parallel Solexa sequencing to identify 11 up-regulated miRNAs and 19 down-regulated miRNAs with computational techniques in the sera of ovarian cancer patients while using healthy serum as the control. Among the above, four miRNAs (miR-22, miR-93, miR-106b, miR-451) were validated by quantitative RT-PCR and found to be significantly aberrantly expressed in the serum of ovarian cancer patients (P<0.05). There were no significant differences between samples from cancer stage I/II and III/IV. However, the levels of miR-106b (p=0.003) and miR-451 (p=0.007) were significantly different in those patients under and over 51 yearsof age. MiR-451 and miR-93 were also specific when analyzed with reference to different levels of CA125. This study shows that Solexa sequencing provides a promising method for cancer-related miRNA profiling, and selectively expressed miRNAs could be used as potential serum-based biomarkers for ovarian cancer diagnosis.
authors.affiliation: Institute of Bioengineering, Zhejiang Sci-Tech University, Hangzhou, China E-mail : xfding@zstu.edu.cn.
authors.forename: Ting
authors.initials: T
authors.lastname: Ji
authors.forename: Zhi-Guo
authors.initials: ZG
authors.lastname: Zheng
authors.forename: Feng-Mei
authors.initials: FM
authors.lastname: Wang
authors.forename: Li-Jian
authors.initials: LJ
authors.lastname: Xu
authors.forename: Lu-Feng
authors.initials: LF
authors.lastname: Li
authors.forename: Qi-Hui
authors.initials: QH
authors.lastname: Cheng
authors.forename: Jiang-Feng
authors.initials: JF
authors.lastname: Guo
authors.forename: Xian-Feng
authors.initials: XF
authors.lastname: Ding
mesh_terms: D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000097:blood; D018394:CA-125 Antigen / Q000097:blood; D005260:Female; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000097:blood; D035683:MicroRNAs / Q000096:biosynthesis / Q000097:blood*; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000097:blood* / Q000175:diagnosis / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D014408:Biomarkers, Tumor; D018394:CA-125 Antigen; C542541:MIRN106 microRNA, human; C548375:MIRN22 microRNA, human; C531201:MIRN451 microRNA, human; C543350:MIRN93 microRNA, human; C451759:MUC16 protein, human; D008565:Membrane Proteins; D035683:MicroRNAs