title: Determination of aqueous two-phase system phase-forming components in the presence of bovine serum albumin.
abstract: In the current work, the quantification of different poly(ethylene glycol) (PEG)-potassium phosphate/sodium citrate aqueous two-phase system (ATPS) phase-forming components was investigated by using conductivity and refractive index measurements. For this purpose, refractive index and conductivity calibration curves were obtained for ATPS at different pH values in the presence of different bovine serum albumin (BSA) concentrations. Whereas BSA had no effect on the conductivity, it had a considerable effect on the refractive index. Finally, a convenient dilution of the samples prior to the ATPS constituent determination is needed to ensure no significant influence from BSA.
authors.affiliation: Institute of Technical Chemistry, Leibniz University of Hannover, 30167 Hannover, Germany.
authors.forename: Anna
authors.initials: A
authors.lastname: Glyk
authors.affiliation: Institute of Technical Chemistry, Leibniz University of Hannover, 30167 Hannover, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Scheper
authors.affiliation: Institute of Technical Chemistry, Leibniz University of Hannover, 30167 Hannover, Germany. Electronic address: beutel@iftc.uni-hannover.de.
authors.forename: Sascha
authors.initials: S
authors.lastname: Beutel
mesh_terms: D001671:Biochemistry / Q000379:methods*; D002138:Calibration; D002951:Citrates / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D010710:Phosphates / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry*; D017680:Potassium Compounds / Q000737:chemistry; D012031:Refractometry; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D000077559:Sodium Citrate; D014867:Water
chemical_list: D002951:Citrates; D010710:Phosphates; D017680:Potassium Compounds; D014867:Water; D000077559:Sodium Citrate; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols; C013216:potassium phosphate
keywords: Aqueous two-phase system; Conductivity measurement; Refractive index measurement