title: Comparative effects of soy phytoestrogens and 17β-estradiol on DNA methylation of a panel of 24 genes in prostate cancer cell lines.
abstract: Major phytoestrogens genistein and daidzein have been reported to have the ability to reverse DNA methylation in cancer cell lines. The mechanism by which genistein and daidzein have an inhibiting action on DNA methylation is not well understood. The aim of this study was to investigate the effects of soy phytoestrogens and the natural estrogen 17β-estradiol (E2) to determine whether one of the estrogen receptors is mobilized for the action of these compounds on DNA methylation. We also made a comparative study with a DNA methylation inhibitor (5-azacytidine) and a DNA methylation activator (budesonide). Three prostate cell lines, PC-3, DU-145, and LNCaP, were treated with 40 μM genistein, 110 μM daidzein, 2 μM budesonide, 2 μM 5-azacytidine, and 10 μM E2. In these 3 human prostate cancer cell lines, we performed methylation quantification using methyl-profiler-DNA-methylation analysis. Soy phytoestrogens and E2 induced a demethylation of all the promoter regions studied except for those that were unmethylated in control cells. Our results showed that E2 induces, like soy phytoestrogen, a decrease in DNA methylation in prostate cancer cell lines. This action may be mediated through ERβ.
authors.affiliation: a Centre Jean Perrin, Department of Oncogenetics, CBRV, Clermont-Ferrand, France and ERTICA, EA 4677 , University of Auvergne , Clermont-Ferrand , France.
authors.forename: Mawussi
authors.initials: M
authors.lastname: Adjakly
authors.forename: Marjolaine
authors.initials: M
authors.lastname: Ngollo
authors.forename: André
authors.initials: A
authors.lastname: Lebert
authors.forename: Aslihan
authors.initials: A
authors.lastname: Dagdemir
authors.forename: Frédérique
authors.initials: F
authors.lastname: Penault-Llorca
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Boiteux
authors.forename: Yves-Jean
authors.initials: YJ
authors.lastname: Bignon
authors.forename: Laurent
authors.initials: L
authors.lastname: Guy
authors.forename: Dominique
authors.initials: D
authors.lastname: Bernard-Gallon
mesh_terms: D001374:Azacitidine / Q000494:pharmacology; D019819:Budesonide / Q000494:pharmacology; D045744:Cell Line, Tumor / Q000187:drug effects; D019175:DNA Methylation / Q000187:drug effects*; D004958:Estradiol / Q000494:pharmacology*; D047629:Estrogen Receptor beta / Q000235:genetics / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D019833:Genistein / Q000494:pharmacology; D006801:Humans; D007529:Isoflavones / Q000494:pharmacology; D008297:Male; D048789:Phytoestrogens / Q000494:pharmacology*; D011401:Promoter Regions, Genetic / Q000187:drug effects; D011471:Prostatic Neoplasms / Q000188:drug therapy / Q000235:genetics*; D013025:Glycine max / Q000737:chemistry*
chemical_list: D047629:Estrogen Receptor beta; D007529:Isoflavones; D048789:Phytoestrogens; D004958:Estradiol; D019819:Budesonide; C004742:daidzein; D019833:Genistein; D001374:Azacitidine