title: High-speed multispectral confocal biomedical imaging.
abstract: A new approach for generating high-speed multispectral confocal images has been developed. The central concept is that spectra can be acquired for each pixel in a confocal spatial scan by using a fast spectrometer based on optical fiber delay lines. This approach merges fast spectroscopy with standard spatial scanning to create datacubes in real time. The spectrometer is based on a serial array of reflecting spectral elements, delay lines between these elements, and a single element detector. The spatial, spectral, and temporal resolution of the instrument is described and illustrated by multispectral images of laser-induced autofluorescence in biological tissues.
authors.affiliation: Omega Optical Inc., 21 Omega Drive, Brattleboro, Vermont 05301.
authors.forename: Gary E
authors.initials: GE
authors.lastname: Carver
authors.affiliation: Omega Optical Inc., 21 Omega Drive, Brattleboro, Vermont 05301.
authors.forename: Sarah A
authors.initials: SA
authors.lastname: Locknar
authors.affiliation: Omega Optical Inc., 21 Omega Drive, Brattleboro, Vermont 05301.
authors.forename: William A
authors.initials: WA
authors.lastname: Morrison
authors.affiliation: Cedars-Sinai Medical Center, 8700 Beverly Boulevard, Los Angeles, California 90048.
authors.forename: V Krishnan
authors.initials: VK
authors.lastname: Ramanujan
authors.affiliation: University of Southern California, Department of Biomedical Engineering, Los Angeles, California 90089dSpectral Molecular Imaging, 250 N. Robertson Boulevard, Beverly Hills, California 90211.
authors.forename: Daniel L
authors.initials: DL
authors.lastname: Farkas
mesh_terms: D000818:Animals; D005336:Fiber Optic Technology; D005456:Fluorescent Dyes; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D051379:Mice; D018613:Microscopy, Confocal / Q000295:instrumentation / Q000379:methods*; D009374:Neoplasms, Experimental; D061848:Optical Imaging / Q000295:instrumentation / Q000379:methods*; D051381:Rats
chemical_list: D005456:Fluorescent Dyes