title: MicroRNA-518d regulates PPARα protein expression in the placentas of females with gestational diabetes mellitus.
abstract: The placenta is thought to have a critical role in the pathogenesis of gestational diabetes mellitus (GDM), as GDM‑associated complications resolve following delivery. Placenta-specific microRNAs (miRNAs) may contribute to the pathology of the development of GDM. The aim of the present study was to evaluate whether the placenta-specific miR-518d contributes to the development of GDM. It was revealed that miR-518d expression was higher in placentas taken from patients with GDM compared with control placentas, whereas the protein levels of the predicted miR-518d target gene, peroxisome proliferator-activated receptor-α (PPARα), were lower in placentas from patients with GDM compared with those from control subjects. It was also demonstrated that PPARα was a direct target of miR-518d with a specific binding site at the seed sequence, which determines target specificity. In the placentas of females with GDM increased levels of miR-518d were negatively correlated with the levels of PPARα protein. As PPARα dysregulation may be related to the development of GDM, it is suggested that upregulation of miR-518d may be associated with the pathogenesis of GDM via an effect on the regulation of PPARα expression.
authors.affiliation: State Key Laboratory of Reproductive Medicine, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing, Jiangsu 210004, P.R. China.
authors.forename: Chun
authors.initials: C
authors.lastname: Zhao
authors.affiliation: Wuxi Maternity and Child Health Hospital Affiliated to Nanjing Medical University, Wuxi, Jiangsu 214002, P.R. China.
authors.forename: Ting
authors.initials: T
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Reproductive Medicine, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing, Jiangsu 210004, P.R. China.
authors.forename: Zhonghua
authors.initials: Z
authors.lastname: Shi
authors.affiliation: State Key Laboratory of Reproductive Medicine, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing, Jiangsu 210004, P.R. China.
authors.forename: Hongjuan
authors.initials: H
authors.lastname: Ding
authors.affiliation: State Key Laboratory of Reproductive Medicine, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing, Jiangsu 210004, P.R. China.
authors.forename: Xiufeng
authors.initials: X
authors.lastname: Ling
mesh_terms: D020413:3' Untranslated Regions; D000328:Adult; D001483:Base Sequence; D001665:Binding Sites; D016022:Case-Control Studies; D002460:Cell Line; D016640:Diabetes, Gestational / Q000235:genetics* / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D035683:MicroRNAs / Q000737:chemistry / Q000235:genetics*; D047493:PPAR alpha / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D021381:Protein Transport; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics; D014162:Transfection
chemical_list: D020413:3' Untranslated Regions; C571457:MIRN518 microRNA, human; D035683:MicroRNAs; D047493:PPAR alpha; D012333:RNA, Messenger