title: Biotin selective polymer nano-films.
abstract: BACKGROUND
  The interaction between biotin and avidin is utilized in a wide range of assay and diagnostic systems. A robust material capable of binding biotin should offer scope in the development of reusable assay materials and biosensor recognition elements.
  
  
  RESULTS
  Biotin-selective thin (3-5 nm) films have been fabricated on hexadecanethiol self assembled monolayer (SAM) coated Au/quartz resonators. The films were prepared based upon a molecular imprinting strategy where N,N'-methylenebisacrylamide and 2-acrylamido-2-methylpropanesulfonic acid were copolymerized and grafted to the SAM-coated surface in the presence of biotin methyl ester using photoinitiation with physisorbed benzophenone. The biotinyl moiety selectivity of the resonators efficiently differentiated biotinylated peptidic or carbohydrate structures from their native counterparts.
  
  
  CONCLUSIONS
  Molecularly imprinted ultra thin films can be used for the selective recognition of biotinylated structures in a quartz crystal microbalance sensing platform. These films are stable for periods of at least a month. This strategy should prove of interest for use in other sensing and assay systems.
authors.forename: Louise
authors.initials: L
authors.lastname: Elmlund
authors.forename: Subramanian
authors.initials: S
authors.lastname: Suriyanarayanan
authors.forename: Jesper G
authors.initials: JG
authors.lastname: Wiklander
authors.forename: Teodor
authors.initials: T
authors.lastname: Aastrup
authors.affiliation: Bioorganic & Biophysical Chemistry Laboratory, Centre for Biomaterials Chemistry, Linnæus University, Kalmar SE-391 82, Sweden. ian.nicholls@lnu.se.
authors.forename: Ian A
authors.initials: IA
authors.lastname: Nicholls
mesh_terms: D000178:Acrylamides / Q000737:chemistry*; D001577:Benzophenones / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods; D001710:Biotin / Q000031:analogs & derivatives / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D019921:Biotinylation; D002241:Carbohydrates / Q000737:chemistry; D054802:Molecular Imprinting / Q000379:methods*; D049329:Nanostructures / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry; D058525:Quartz Crystal Microbalance Techniques / Q000379:methods*
chemical_list: D000178:Acrylamides; D001577:Benzophenones; D002241:Carbohydrates; D010455:Peptides; D001710:Biotin; C047723:benzophenone; C021221:N,N'-methylenebisacrylamide; C411823:biotin methyl ester