title: Antithrombotic effects of pyridinium compounds formed from trigonelline upon coffee roasting.
abstract: Coffee may exert a preventive effect on arterial thrombosis. Trigonelline is one of the most abundant compounds in coffee that undergoes pyrolysis upon roasting of coffee beans. The aim of the present study was to identify pyridinium compounds formed upon trigonelline pyrolysis and coffee roasting and to investigate the effect of three of them, i.e., 1-methylpyridine and 1,3- and 1,4-dimethylpyridine, on experimentally induced arterial thrombosis in rats. 1,3- and 1,4-dimethylpyridine but not 1-methylpyridine inhibited arterial thrombus formation. 1,3-Dimethylpyridine inhibited platelet aggregation and reduced fibrin formation in platelet-rich plasma, whereas 1,4-dimethylpyridine increased the plasma level of 6-keto-PGF1α. 1,4-Dimethylpyridine slightly increased rat tissue plasminogen activator plasma activity. In summary, we demonstrated that pyridinium compounds display mild antithrombotic properties due to stimulation by prostacyclin release (1,4-dimethylpyridine) and inhibition of platelet aggregation (1,3-dimethylpyridine). Those pyridinium compounds may, to some extent, be responsible for the beneficial effects of coffee drinking.
authors.affiliation: Department of Pharmacodynamics, Medical University of Bialystok , Mickiewicza Str. 2C, 15-222 Bialystok, Poland.
authors.forename: Bartlomiej
authors.initials: B
authors.lastname: Kalaska
authors.forename: Lukasz
authors.initials: L
authors.lastname: Piotrowski
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Leszczynska
authors.forename: Bartosz
authors.initials: B
authors.lastname: Michalowski
authors.forename: Karol
authors.initials: K
authors.lastname: Kramkowski
authors.forename: Tomasz
authors.initials: T
authors.lastname: Kaminski
authors.forename: Jan
authors.initials: J
authors.lastname: Adamus
authors.forename: Andrzej
authors.initials: A
authors.lastname: Marcinek
authors.forename: Jerzy
authors.initials: J
authors.lastname: Gebicki
authors.forename: Andrzej
authors.initials: A
authors.lastname: Mogielnicki
authors.forename: Wlodzimierz
authors.initials: W
authors.lastname: Buczko
mesh_terms: D000470:Alkaloids / Q000008:administration & dosage / Q000737:chemistry*; D000818:Animals; D040503:Coffea / Q000737:chemistry*; D003296:Cooking; D005343:Fibrinolytic Agents / Q000008:administration & dosage* / Q000737:chemistry; D006801:Humans; D008297:Male; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry; D010974:Platelet Aggregation / Q000187:drug effects; D011726:Pyridinium Compounds / Q000008:administration & dosage* / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D013927:Thrombosis / Q000097:blood / Q000517:prevention & control*
chemical_list: D000470:Alkaloids; D005343:Fibrinolytic Agents; D010936:Plant Extracts; D011726:Pyridinium Compounds; C009560:trigonelline