title: Effect of land application of manure from enrofloxacin-treated chickens on ciprofloxacin resistance of Enterobacteriaceae in soil.
abstract: A field plot experiment was carried out to evaluate the impact of spreading chicken manure containing enrofloxacin (ENR) and its metabolite ciprofloxacin (CIP), on the levels of CIP-resistant Enterobacteriaceae in soil. The manures from chickens treated with ENR and from untreated control chickens were applied on six plots. Total and CIP-resistant Enterobacteriaceae were counted on Violet Red Bile Glucose medium containing 0 to 16mg L(-1) of CIP. A total of 145 isolates were genotyped by enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR). The minimum inhibitory concentration (MIC) of CIP for the isolates of each ERIC-PCR profile was determined. The most frequently isolated Enterobacteriaceae included Escherichia coli, and to a lesser extent, Enterobacter and 5 other genera from environmental origin. The composition of the E. coli community differed between manure and manured soil suggesting that the E. coli genotypes determined by ERIC-PCR varied significantly in their ability to survive in soil. One of these genotypes, including both susceptible and resistant isolates, was detected up to 89 days after the manure was applied. Most of the E. coli isolated in soil amended with manure from treated chickens was resistant to CIP (with a MIC ranging between 2 and 32mg L(-1)). In contrast, despite the presence of ENR in soil at concentrations ranging from 13-518μg kg(-1), the environmental Enterobacteriaceae isolates had a CIP MIC≤0.064mg L(-1), except one isolate which had a MIC of 0.25mg L(-1), These results showed that spreading manure from ENR-treated chickens enabled CIP-resistant E. coli to persist for at least three months in the soil. However, neither the presence of fluoroquinolones, nor the persistence of CIP-resistant E. coli, increased the CIP-susceptibility of environmental Enterobacteriaceae.
authors.affiliation: IRSTEA, 17 Avenue de Cucillé, 35044 Rennes, France; Université Européenne de Bretagne, France. Electronic address: anne-marie.pourcher@irstea.fr.
authors.forename: A-M
authors.initials: AM
authors.lastname: Pourcher
authors.affiliation: Université d'Angers, LETG-Angers LEESA UMR CNRS 6554, UFR Sciences, 2 Boulevard Lavoisier, 49045 Angers, France.
authors.forename: A
authors.initials: A
authors.lastname: Jadas-Hécart
authors.affiliation: Chambre Régionale d'Agriculture de Bretagne, ZAC Atalante Champeaux, 35042 Rennes, France.
authors.forename: P
authors.initials: P
authors.lastname: Cotinet
authors.affiliation: IRSTEA, 17 Avenue de Cucillé, 35044 Rennes, France; Université Européenne de Bretagne, France.
authors.forename: P
authors.initials: P
authors.lastname: Dabert
authors.affiliation: IRSTEA, 17 Avenue de Cucillé, 35044 Rennes, France; Université Européenne de Bretagne, France.
authors.forename: C
authors.initials: C
authors.lastname: Ziebal
authors.affiliation: IRSTEA, 17 Avenue de Cucillé, 35044 Rennes, France; Université Européenne de Bretagne, France.
authors.forename: S
authors.initials: S
authors.lastname: Le Roux
authors.affiliation: University of Agricultural Sciences and Veterinary Medicine, Aleea Mihail Sadoveanu no. 8, 700489 Iaşi, Romania.
authors.forename: R
authors.initials: R
authors.lastname: Moraru
authors.affiliation: Chambre Régionale d'Agriculture de Bretagne, ZAC Atalante Champeaux, 35042 Rennes, France.
authors.forename: D
authors.initials: D
authors.lastname: Heddadj
authors.affiliation: Université Européenne de Bretagne, France; ANSES Laboratoire de Ploufragan/Plouzané, BP 53, 22440 Ploufragan, France.
authors.forename: I
authors.initials: I
authors.lastname: Kempf
mesh_terms: D000822:Animal Husbandry; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology* / Q000627:therapeutic use; D002939:Ciprofloxacin / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial / Q000235:genetics; D000077422:Enrofloxacin; D004755:Enterobacteriaceae / Q000145:classification / Q000235:genetics / Q000254:growth & development*; D024841:Fluoroquinolones / Q000627:therapeutic use; D008372:Manure / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D012037:Refuse Disposal; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology
chemical_list: D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; D008372:Manure; D012987:Soil; D000077422:Enrofloxacin; D002939:Ciprofloxacin
keywords: Chicken manure; Ciprofloxacin; ERIC-PCR; Enrofloxacin; Enterobacteriaceae; Soil