title: Repeated TALEs: visualizing DNA sequence localization and chromosome dynamics in live cells.
abstract: Three recent papers, published just weeks apart, describe the use of fluorescent TALEs to tag specific DNA sequences in live cells and, in one case, also in fixed cells, the latter with potential clinical applications.
authors.affiliation: Program in Cell and Developmental Dynamics; Department of Biochemistry and Molecular Pharmacology; University of Massachusetts Medical School; Worcester, MA USA.
authors.forename: Thoru
authors.initials: T
authors.lastname: Pederson
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000737:chemistry* / Q000378:metabolism*; D002843:Chromatin / Q000378:metabolism*; D002877:Chromosomes, Human / Q000235:genetics*; D004276:DNA, Satellite / Q000032:analysis*; D004268:DNA-Binding Proteins; D005456:Fluorescent Dyes / Q000378:metabolism*; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods*; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D013194:Staining and Labeling / Q000379:methods*; D015533:Transcriptional Activation / Q000235:genetics*
chemical_list: D002843:Chromatin; D004276:DNA, Satellite; D004268:DNA-Binding Proteins; D005456:Fluorescent Dyes
keywords: DNA replication timing; cell cycle; centromeres; single nucleotide polymorphisms; telomeres; transcription activator-like effectors