title: Abnormal levels of expression of plasma microRNA-33 in patients with psoriasis.
abstract: OBJECTIVE
  Circulating microRNAs (miRNA) are involved in the posttranscriptional regulation of genes associated with lipid metabolism (miRNA-33) and vascular function and angiogenesis (miRNA-126). The objective of this exploratory study was to measure plasma levels of miRNA-33 and miRNA-126 in patients with plaque psoriasis and evaluate their association with clinical parameters.
  
  
  METHODS
  We studied 11 patients with plaque psoriasis. The median Psoriasis Area Severity Index (PASI) was 13 (interquartile range [IQR], 9-14) and body surface area involvement was 12 (IQR, 11-15). Eleven healthy controls matched for age and sex were also included. We analyzed cardiovascular risk factors and subclinical carotid atheromatosis. Plasma miRNAs were evaluated using quantitative real-time polymerase chain reaction.
  
  
  RESULTS
  Carotid intima-media thickness was greater in patients (0.57mm; IQR, 0.54-0.61; n=11) than in controls (0.50mm; IQR, 0.48-0.54; data available for 9 controls) (P=.0055, Mann-Whitney). Expression of miRNA-33 in patients (5.34; IQR, 3.12-7.96; n=11) was significantly higher than in controls (2.33; IQR, 1.71-2.84; only detected in 7 of 11 controls) (P=.0049, Wilcoxon signed rank). No differences in miRNA-126 levels were observed between patients and controls. In patients (n=11), we observed a positive correlation between miRNA-33 and insulin levels (r=0.7289, P=.0109) and a negative correlation between miRNA-126 and carotid intima-media thickness (r=-0.6181, P=.0426).
  
  
  CONCLUSIONS
  In psoriasis patients plasma levels of lipid and glucose metabolism-related miRNA-33 are increased and correlated with insulin. The study of circulating miRNA-33 in psoriasis may provide new insights about the associated systemic inflammatory abnormalities.
authors.affiliation: Departamento de Biología Celular e Inmunología, Instituto de Parasitología y Biomedicina López-Neyra, IPBLN, Consejo Superior de Investigaciones Científicas (CSIC), Parque Tecnológico Ciencias de la Salud, Armilla, Granada, España.
authors.forename: S
authors.initials: S
authors.lastname: García-Rodríguez
authors.affiliation: Departamento de Dermatología, Hospital Universitario San Cecilio (HCUSC), Granada, España; Departamento de Dermatología, Hospital Virgen de las Nieves, Granada, España.
authors.forename: S
authors.initials: S
authors.lastname: Arias-Santiago
authors.affiliation: Departamento de Dermatología, Hospital Universitario San Cecilio (HCUSC), Granada, España.
authors.forename: J
authors.initials: J
authors.lastname: Orgaz-Molina
authors.affiliation: Departamento de Reumatología, Hospital Universitario San Cecilio (HCUSC), Granada, España.
authors.forename: C
authors.initials: C
authors.lastname: Magro-Checa
authors.affiliation: Departamento de Dermatología, Hospital Virgen de las Nieves, Granada, España.
authors.forename: I
authors.initials: I
authors.lastname: Valenzuela
authors.affiliation: Departamento de Biología Celular e Inmunología, Instituto de Parasitología y Biomedicina López-Neyra, IPBLN, Consejo Superior de Investigaciones Científicas (CSIC), Parque Tecnológico Ciencias de la Salud, Armilla, Granada, España.
authors.forename: P
authors.initials: P
authors.lastname: Navarro
authors.affiliation: Departamento de Dermatología, Hospital Universitario San Cecilio (HCUSC), Granada, España.
authors.forename: R
authors.initials: R
authors.lastname: Naranjo-Sintes
authors.affiliation: Departamento de Biología Celular e Inmunología, Instituto de Parasitología y Biomedicina López-Neyra, IPBLN, Consejo Superior de Investigaciones Científicas (CSIC), Parque Tecnológico Ciencias de la Salud, Armilla, Granada, España.
authors.forename: J
authors.initials: J
authors.lastname: Sancho
authors.affiliation: Departamento de Biología Celular e Inmunología, Instituto de Parasitología y Biomedicina López-Neyra, IPBLN, Consejo Superior de Investigaciones Científicas (CSIC), Parque Tecnológico Ciencias de la Salud, Armilla, Granada, España. Electronic address: mzubiaur@ipb.csic.es.
authors.forename: M
authors.initials: M
authors.lastname: Zubiaur
mesh_terms: D000328:Adult; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics; D011565:Psoriasis / Q000097:blood* / Q000235:genetics
chemical_list: C527111:MIRN126 microRNA, human; C550416:MIRN33a microRNA, human; D035683:MicroRNAs
keywords: Carotid intima-media thickness; Insulin; Insulina; Media del grosor de la íntima carotídea; Psoriasis; miARN-126; miARN-33; miRNA-126; miRNA-33