title: Chitosan-based copper nanocomposite accelerates healing in excision wound model in rats.
abstract: Copper possesses efficacy in wound healing which is a complex phenomenon involving various cells, cytokines and growth factors. Copper nanoparticles modulate cells, cytokines and growth factors involved in wound healing in a better way than copper ions. Chitosan has been shown to be beneficial in healing because of its antibacterial, antifungal, biocompatible and biodegradable polymeric nature. In the present study, chitosan-based copper nanocomposite (CCNC) was prepared by mixing chitosan and copper nanoparticles. CCNC was applied topically to evaluate its wound healing potential and to study its effects on some important components of healing process in open excision wound model in adult Wistar rats. Significant increase in wound contraction was observed in the CCNC-treated rats. The up-regulation of vascular endothelial growth factor (VEGF) and transforming growth factor-beta1(TGF-β1) by CCNC-treatment revealed its role in facilitating angiogenesis, fibroblast proliferation and collagen deposition. The tumor necrosis factor-α (TNF-α) and interleukin-10 (IL-10) were significantly decreased and increased, respectively, in CCNC-treated rats. Histological evaluation showed more fibroblast proliferation, collagen deposition and intact re-epithelialization in CCNC-treated rats. Immunohistochemistry of CD31 revealed marked increase in angiogenesis. Thus, we concluded that chitosan-based copper nanocomposite efficiently enhanced cutaneous wound healing by modulation of various cells, cytokines and growth factors during different phases of healing process.
authors.affiliation: Division of Pharmacology and Toxicology, Indian Veterinary Research Institute, Izatnagar 243 122, Uttar Pradesh, India.
authors.forename: Anu
authors.initials: A
authors.lastname: Gopal
authors.affiliation: Division of Pharmacology and Toxicology, Indian Veterinary Research Institute, Izatnagar 243 122, Uttar Pradesh, India.
authors.forename: Vinay
authors.initials: V
authors.lastname: Kant
authors.affiliation: Division of Pharmacology and Toxicology, Indian Veterinary Research Institute, Izatnagar 243 122, Uttar Pradesh, India.
authors.forename: Anu
authors.initials: A
authors.lastname: Gopalakrishnan
authors.affiliation: Division of Pharmacology and Toxicology, Indian Veterinary Research Institute, Izatnagar 243 122, Uttar Pradesh, India.
authors.forename: Surendra K
authors.initials: SK
authors.lastname: Tandan
authors.affiliation: Division of Pharmacology and Toxicology, Indian Veterinary Research Institute, Izatnagar 243 122, Uttar Pradesh, India. Electronic address: dineshks17@gmail.com.
authors.forename: Dinesh
authors.initials: D
authors.lastname: Kumar
mesh_terms: D019342:Acetic Acid / Q000494:pharmacology; D000818:Animals; D048271:Chitosan / Q000737:chemistry* / Q000494:pharmacology*; D003300:Copper / Q000737:chemistry*; D005786:Gene Expression Regulation / Q000187:drug effects; D016753:Interleukin-10 / Q000378:metabolism; D008297:Male; D053768:Metal Nanoparticles / Q000737:chemistry; D053761:Nanocomposites / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000166:cytology / Q000293:injuries; D013997:Time Factors; D053773:Transforming Growth Factor beta1 / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D053773:Transforming Growth Factor beta1; D014409:Tumor Necrosis Factor-alpha; D042461:Vascular Endothelial Growth Factor A; D016753:Interleukin-10; D003300:Copper; D048271:Chitosan; D019342:Acetic Acid
keywords: Chitosan; Copper; TGF-β1; TNF-α; VEGF; Wound healing