title: Eosinophilic granuloma with Splendore-Hoeppli material caused by toxigenic Corynebacterium ulcerans in a heifer.
abstract: Raised lesions were present on the left nasal vestibule of a 20-month-old Japanese Brown heifer. The largest mass which caused partial nasal obstruction was removed surgically. Corynebacterium ulcerans was identified in the mass. 16S ribosomal RNA and RNA polymerase beta subunit genes were 100% and 98% identical to other C. ulcerans strains. Histologically, multiple foci of eosinophilic granuloma with Splendore-Hoeppli material were seen. Rod-shaped Gram-positive organisms were detected with metachromatic granules, producing diphtheria toxin with 5, 30 and 48 amino acid differences to another C. ulcerans strain, C. diphtheriae or C. pseudotuberculosis, respectively. The toxin is highly cytotoxic and may be responsible for the formation of abundant Splendore-Hoeppli material. The lesion was therefore judged to be an allergic reaction to bacterial antigens or diphtheria toxin.
authors.affiliation: Naka-Sorachi Agricultural Mutual Aid Association, 1-5-14 Omachi, Takikawa 073-0022, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Murakami
authors.forename: Eiji
authors.initials: E
authors.lastname: Hata
authors.forename: Shinichi
authors.initials: S
authors.lastname: Hatama
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Wada
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Ito
authors.forename: Yoshiharu
authors.initials: Y
authors.lastname: Ishikawa
authors.forename: Koichi
authors.initials: K
authors.lastname: Kadota
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology* / Q000473:pathology* / Q000601:surgery; D003352:Corynebacterium / Q000737:chemistry* / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D004167:Diphtheria Toxin / Q000032:analysis* / Q000235:genetics; D004803:Eosinophilic Granuloma / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D005260:Female; D006652:Histological Techniques / Q000662:veterinary; D007150:Immunohistochemistry / Q000662:veterinary; D009669:Nose Neoplasms / Q000382:microbiology / Q000473:pathology / Q000601:surgery / Q000662:veterinary*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D004167:Diphtheria Toxin; D012336:RNA, Ribosomal, 16S; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit