title: Meta-analysis: diagnostic accuracy of antinuclear antibodies, smooth muscle antibodies and antibodies to a soluble liver antigen/liver pancreas in autoimmune hepatitis.
abstract: BACKGROUND
  Antinuclear antibodies (ANA), smooth muscle antibodies (SMA) and antibodies to a soluble liver antigen/liver pancreas (anti-SLA/LP) are useful markers that can help clinicians to diagnose and classify autoimmune hepatitis (AIH).
  
  
  OBJECTIVE
  To determine whether ANA, SMA and anti-SLA/LP help to accurately diagnose patients with AIH.
  
  
  METHODS
  The PubMed, CNKI, WANFANG, and SinoMed databases were accessed to retrieve studies published in English and Chinese. Studies published up to October 2013 were reviewed.
  
  
  METHODS
  Studies on the diagnostic value of ANA, SMA or anti-SLA/LP in the diagnosis of known or suspected AIH were included.
  
  
  METHODS
  Two authors evaluated studies independently and rated their methodological quality using quality assessment of diagnostic accuracy studies (QUADAS) tools; relevant data were abstracted. The random-effects method was used to summarize sensitivities, specificities, positive and negative likelihood ratios, and diagnostic odds ratios (DORs) from all 29 studies.
  
  
  RESULTS
  The pooled sensitivity, specificity, positive and negative likelihood ratios, and DOR for ANA were 0.650 (95% confidence interval [CI], 0.619 to 0.680), 0.751 (95%CI, 0.737 to 0.764), 3.030 (95%CI, 2.349 to 3.910), 0.464 (95%CI, 0.356 to 0.604), and 7.380 (95%CI, 4.344 to 12.539), respectively. For SMA, the values were 0.593 (95%CI, 0.564 to 0.621), 0.926 (95%CI, 0.917 to 0.934), 11.740 (95%CI, 7.379 to 18.678), 0.449 (95%CI, 0.367 to 0.549), and 31.553 (95%CI, 17.147 to 58.060), respectively. Finally, for anti-SLA/LP, the values were 0.194 (95%CI, 0.168 to 0.222), 0.989 (95%CI, 0.985 to 0.993), 11.089 (95%CI, 7.601 to 16.177), 0.839 (95%CI, 0.777 to 0.905), and 16.867 (95%CI, 10.956 to 25.967), respectively.
  
  
  CONCLUSIONS
  ANA provided moderate sensitivity and specificity, while SMA gave moderate sensitivity and high specificity, and anti-SLA/LP exhibited low sensitivity and high specificity. All three antibodies were limited by their unsatisfactory sensitivities and lack of consistency.
authors.affiliation: The Department of Laboratory Medicine, the Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.
authors.forename: Wen-Chao
authors.initials: WC
authors.lastname: Zhang
authors.affiliation: The Department of Gynaecology and Obstetrics, Youyang People's Hospital, Chongqing, China.
authors.forename: Feng-Rong
authors.initials: FR
authors.lastname: Zhao
authors.affiliation: Institute of Viral Hepatitis, the Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Chen
authors.affiliation: The Department of Laboratory Medicine, the Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.
authors.forename: Wei-Xian
authors.initials: WX
authors.lastname: Chen
mesh_terms: D000974:Antibodies, Antinuclear / Q000276:immunology*; D001324:Autoantigens / Q000276:immunology*; D019693:Hepatitis, Autoimmune / Q000175:diagnosis* / Q000276:immunology*; D006801:Humans; D009130:Muscle, Smooth / Q000276:immunology*; D017594:Publication Bias; D012372:ROC Curve; D012680:Sensitivity and Specificity
chemical_list: D000974:Antibodies, Antinuclear; D001324:Autoantigens; C046431:liver antigen LA-1