title: Development of multilocus variable-number tandem repeat analysis (MLVA) for Xanthomonas arboricola pathovars.
abstract: Xanthomonas arboricola is an important bacterial species, the pathovars of which are responsible for bacterial blight diseases on stone fruit, hazelnut, Persian walnut, poplar, strawberry, poinsettia and banana. In this study, we evaluated variable number tandem repeats (VNTR) as a molecular typing tool for assessing the genetic diversity within pathovars of X. arboricola. Screening of the X. arboricola pv. pruni genome sequence (CFBP5530 strain) predicted 51 candidate VNTR loci. Primer pairs for polymerase chain reaction (PCR) amplification of all 51 loci were designed, and their discriminatory power was initially evaluated with a core collection of 8 X. arboricola strains representative of the different pathovars. Next, the 26 polymorphic VNTR loci present in all strains were used for genotyping a collection of 61 strains. MLVA is a typing method that clearly differentiates X. arboricola strains. The MLVA scheme described in this study is a rapid and reliable molecular typing tool that can be used for further epidemiological studies of bacterial diseases caused by X. arboricola pathovars.
authors.affiliation: INRA, UMR1345 IRHS, F-49071 Beaucouzé, France; AGROCAMPUS OUEST, UMR1345 IRHS, F-49071 Beaucouzé, France; Université d'Angers, UMR1345 IRHS, SFR 4207 QUASAV, PRES L'UNAM, F-49071 Beaucouzé, France. Electronic address: sophie.cesbron@angers.inra.fr.
authors.forename: Sophie
authors.initials: S
authors.lastname: Cesbron
authors.affiliation: Agroscope Changins-Wädenswil Research Station ACW, Plant Protection Division, Schloss 1, CH-8820 Wädenswil, Switzerland.
authors.forename: Joel
authors.initials: J
authors.lastname: Pothier
authors.affiliation: INRA, UMR1345 IRHS, F-49071 Beaucouzé, France; AGROCAMPUS OUEST, UMR1345 IRHS, F-49071 Beaucouzé, France; Université d'Angers, UMR1345 IRHS, SFR 4207 QUASAV, PRES L'UNAM, F-49071 Beaucouzé, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Gironde
authors.affiliation: INRA, UMR1345 IRHS, F-49071 Beaucouzé, France; AGROCAMPUS OUEST, UMR1345 IRHS, F-49071 Beaucouzé, France; Université d'Angers, UMR1345 IRHS, SFR 4207 QUASAV, PRES L'UNAM, F-49071 Beaucouzé, France.
authors.forename: Marie-Agnès
authors.initials: MA
authors.lastname: Jacques
authors.affiliation: INRA, UMR1345 IRHS, F-49071 Beaucouzé, France; AGROCAMPUS OUEST, UMR1345 IRHS, F-49071 Beaucouzé, France; Université d'Angers, UMR1345 IRHS, SFR 4207 QUASAV, PRES L'UNAM, F-49071 Beaucouzé, France.
authors.forename: Charles
authors.initials: C
authors.lastname: Manceau
mesh_terms: D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D005838:Genotype; D018598:Minisatellite Repeats; D017720:Molecular Epidemiology / Q000379:methods; D058889:Molecular Typing / Q000379:methods*; D010935:Plant Diseases / Q000382:microbiology; D014974:Xanthomonas / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial
keywords: Genetic diversity; MLVA; Molecular epidemiology; Xanthomonas arboricola