title: Tributyltin induces cell cycle arrest at G1 phase in the yeast Saccharomyces cerevisiae.
abstract: Tributyltin (TBT) has long been recognized as a major environmental pollutant that can cause significant damage to the cellular functions as well as disruption of endocrine homeostasis. TBT induces apoptosis accompanied by production of reactive oxygen species (ROS) in mammalian and yeast cells. We observed that the budding yeast cells exposed to this compound at low concentrations exhibited cell growth arrest, but not cell death. Flow cytometric analysis of yeast cells without synchronization and morphological assessment of cells synchronized at M phase by nocodazole treatment indicated that TBT-exposed Saccharomyces cerevisiae cells were arrested at G1 phase of the cell cycle. This arrest was recovered by the addition of N-acetylcysteine, suggesting the involvement of ROS production by TBT. This is the first study to evaluate the action of TBT on cell cycle events.
authors.affiliation: Faculty of Agriculture, Ehime University.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Sekito
authors.forename: Naoko
authors.initials: N
authors.lastname: Sugimoto
authors.forename: Masaya
authors.initials: M
authors.lastname: Ishimoto
authors.forename: Miyuki
authors.initials: M
authors.lastname: Kawano-Kawada
authors.forename: Koichi
authors.initials: K
authors.lastname: Akiyama
authors.forename: Sogo
authors.initials: S
authors.lastname: Nishimoto
authors.forename: Takuya
authors.initials: T
authors.lastname: Sugahara
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Kakinuma
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D059447:Cell Cycle Checkpoints / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004785:Environmental Pollutants / Q000633:toxicity*; D016193:G1 Phase / Q000187:drug effects*; D015739:Nocodazole / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000187:drug effects*; D014220:Trialkyltin Compounds / Q000633:toxicity*
chemical_list: D004785:Environmental Pollutants; D017382:Reactive Oxygen Species; D014220:Trialkyltin Compounds; C011559:tributyltin; D015739:Nocodazole