title: In vitro differentiation of murine hematopoietic progenitor cells toward the myeloid lineage occurs in response to Staphylococcus aureus and yeast species.
abstract: We have studied the effect of inactivated microbial stimuli (Candida albicans, Candida glabrata, Saccharomyces boulardii, and Staphylococcus aureus) on the in vitro differentiation of lineage negative (Lin(-)) hematopoietic progenitor mouse cells. Purified Lin(-) progenitors were co-cultured for 7 days with the stimuli, and cell differentiation was determined by flow cytometry analysis. All the stimuli assayed caused differentiation toward the myeloid lineage. S. boulardii and particularly C. glabrata were the stimuli that induced in a minor extent differentiation of Lin(-) cells, as the major population of differentiated cells corresponded to monocytes, whereas C. albicans and S. aureus induced differentiation beyond monocytes: to monocyte-derived dendritic cells and macrophages, respectively. Interestingly, signaling through TLR2 by its pure ligand Pam3CSK4 directed differentiation of Lin(-) cells almost exclusively to macrophages. These data support the notion that hematopoiesis can be modulated in response to microbial stimuli in a pathogen-dependent manner, being determined by the pathogen-associated molecular patterns and the pattern-recognition receptors involved, in order to generate the populations of mature cells required to deal with the pathogen.
authors.affiliation: Departamento de Óptica, Farmacología y Anatomía, Universidad de Alicante, Alicante, Spain.
authors.forename: Victoria
authors.initials: V
authors.lastname: Maneu
authors.affiliation: Departamento de Microbiología y Ecología, Universitat de València, Burjassot, Spain.
authors.forename: Miguel Ángel
authors.initials: MÁ
authors.lastname: Estévez
authors.affiliation: Departamento de Microbiología y Ecología, Universitat de València, Burjassot, Spain.
authors.forename: Sara
authors.initials: S
authors.lastname: de Dios
authors.affiliation: Departamento de Microbiología y Ecología, Universitat de València, Burjassot, Spain.
authors.forename: Daniel
authors.initials: D
authors.lastname: Gozalbo
authors.affiliation: Departamento de Microbiología y Ecología, Universitat de València, Burjassot, Spain.
authors.forename: María Luisa
authors.initials: ML
authors.lastname: Gil
authors.affiliation: Departamento de Microbiología y Ecología, Universitat de València, Burjassot, Spain. Electronic address: javier.megias@uv.es.
authors.forename: Javier
authors.initials: J
authors.lastname: Megías
mesh_terms: D000818:Animals; D002176:Candida albicans / Q000502:physiology*; D041221:Candida glabrata / Q000502:physiology*; D002454:Cell Differentiation; D018920:Coculture Techniques; D005260:Female; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D008810:Mice, Inbred C57BL; D051192:Receptors, Pattern Recognition / Q000378:metabolism; D012440:Saccharomyces / Q000502:physiology*; D013211:Staphylococcus aureus / Q000502:physiology*
chemical_list: D051192:Receptors, Pattern Recognition
keywords: Candida albicans; Candida glabrata; Mouse hematopoietic progenitors; Pattern-recognition receptors; Saccharomyces boulardii; Staphylococcus aureus