title: A new role for Escherichia coli DsbC protein in protection against oxidative stress.
abstract: We report a new function for Escherichia coli DsbC, a protein best known for disulfide bond isomerization in the periplasm. We found that DsbC regulates the redox state of the single cysteine of the L-arabinose-binding protein AraF. This cysteine, which can be oxidized to a sulfenic acid, mediates the formation of a disulfide-linked homodimer under oxidative stress conditions, preventing L-arabinose binding. DsbC, unlike the homologous protein DsbG, reduces the intermolecular disulfide, restoring AraF binding properties. Thus, our results reveal a new link between oxidative protein folding and the defense mechanisms against oxidative stress.
authors.affiliation: From the de Duve Institute, Université catholique de Louvain, 1200 Brussels, Belgium.
authors.forename: Katleen
authors.initials: K
authors.lastname: Denoncin
authors.forename: Didier
authors.initials: D
authors.lastname: Vertommen
authors.forename: Isabelle S
authors.initials: IS
authors.lastname: Arts
authors.forename: Camille V
authors.initials: CV
authors.lastname: Goemans
authors.forename: Sophie
authors.initials: S
authors.lastname: Rahuel-Clermont
authors.forename: Joris
authors.initials: J
authors.lastname: Messens
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Collet
mesh_terms: D000595:Amino Acid Sequence; D001089:Arabinose / Q000378:metabolism; D015153:Blotting, Western; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D003512:Cyclohexanones / Q000494:pharmacology; D003545:Cysteine / Q000378:metabolism; D004220:Disulfides / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress; D011485:Protein Binding; D019704:Protein Disulfide-Isomerases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D055503:Protein Multimerization; D017434:Protein Structure, Tertiary; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity; D013434:Sulfenic Acids / Q000378:metabolism
chemical_list: C016242:AraF protein, E coli; D002352:Carrier Proteins; D003512:Cyclohexanones; D004220:Disulfides; D029968:Escherichia coli Proteins; D013434:Sulfenic Acids; C004774:dimedone; D001089:Arabinose; D019704:Protein Disulfide-Isomerases; C526649:dsbC protein, E coli; D003545:Cysteine
keywords: Disulfide; DsbC; Escherichia coli; Oxidative Stress; Protein Folding; Redox Regulation; Sulfenic Acid; Thioredoxin