title: [Antimicrobial susceptibility testing of anaerobic bacteria].
abstract: The anaerobic bacteria resistance to antibiotics is increasing, and even has appeared against the most active of those, like metronidazol and carbapenems. This fact forces to make and periodical sensibility tests -at least in the most aggressive and virulent species, in cases that they are isolated from life locations and in the absence of therapeutic response- to check the local sensibility and to establish suitable empiric therapies, all based on multicentric studies carried out in order to this or well to check the activity of new antibiotics. For the laboratory routine, the easiest sensibility method is the E-test/MIC evaluator. Another alternative is microdilution, that's only normalized for Bacteroides. There are preliminary facts that allow the use of disc diffusion method in some species of Bacteroides and Clostridium. For the temporal and multicentric studies, the procedure is dilution in agar plate, the reference method.
authors.affiliation: Servicio de Microbiología, Complejo Asistencial Universitario de Salamanca, Salamanca, España; Departamento de Medicina Preventiva, Salud Pública y Microbiología Médica, Facultad de Medicina, Universidad de Salamanca, Salamanca, España. Electronic address: joegas@usal.es.
authors.forename: José E
authors.initials: JE
authors.lastname: García-Sánchez
authors.affiliation: Departamento de Medicina Preventiva, Salud Pública y Microbiología Médica, Facultad de Medicina, Universidad de Salamanca, Salamanca, España.
authors.forename: Enrique
authors.initials: E
authors.lastname: García-Sánchez
authors.affiliation: Servicio de Microbiología, Complejo Asistencial Universitario de Salamanca, Salamanca, España; Departamento de Medicina Preventiva, Salud Pública y Microbiología Médica, Facultad de Medicina, Universidad de Salamanca, Salamanca, España.
authors.forename: María Inmaculada
authors.initials: MI
authors.lastname: García-García
mesh_terms: D001421:Bacteria, Anaerobic / Q000187:drug effects*; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods
keywords: Agar dilution method; Anaerobic bacteria; Bacterias anaerobias; Broth microdilution method; Commercial systems for antimicrobial susceptibility testing; Diffusion from method; Difusión a partir de tiras; Dilución en agar; Disk diffusion method; Methods for antimicrobial susceptibility testing; Microdilución en caldo; Método disco placa; Métodos de sensibilidad; Sistemas comerciales