title: Determinants for membrane association of the hepatitis C virus NS2 protease domain.
abstract: Hepatitis C virus (HCV) nonstructural protein 2 (NS2) is required for HCV polyprotein processing and particle assembly. It comprises an N-terminal membrane domain and a C-terminal, cytosolically oriented protease domain. Here, we demonstrate that the NS2 protease domain itself associates with cellular membranes. A single charged residue in the second α-helix of the NS2 protease domain is required for proper membrane association, NS2 protein stability, and efficient HCV polyprotein processing.
authors.affiliation: Division of Gastroenterology and Hepatology, Centre Hospitalier Universitaire Vaudois, University of Lausanne, Switzerland.
authors.forename: Christian M
authors.initials: CM
authors.lastname: Lange
authors.forename: Pantxika
authors.initials: P
authors.lastname: Bellecave
authors.forename: Viet Loan
authors.initials: VL
authors.lastname: Dao Thi
authors.forename: Huong T L
authors.initials: HT
authors.lastname: Tran
authors.forename: François
authors.initials: F
authors.lastname: Penin
authors.forename: Darius
authors.initials: D
authors.lastname: Moradpour
authors.forename: Jérôme
authors.initials: J
authors.lastname: Gouttenoire
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002462:Cell Membrane / Q000378:metabolism*; D049452:Green Fluorescent Proteins; D016174:Hepacivirus / Q000201:enzymology*; D018613:Microscopy, Confocal; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary / Q000235:genetics; D017422:Sequence Analysis, DNA; D017361:Viral Nonstructural Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D019065:Virus Assembly / Q000235:genetics / Q000502:physiology*
chemical_list: C097010:NS2 protein, Hepatitis C virus; D017361:Viral Nonstructural Proteins; D049452:Green Fluorescent Proteins