title: Isoflavones in aglycone solution enhance ultraviolet B-induced DNA damage repair efficiency.
abstract: The isoflavones daidzein and genistein are natural compounds which have anti-inflammatory and photoprotective activities, and may be effective in the repair of ultraviolet (UV)-induced photodamage. In this study, an alcoholic solution of aglycone isoflavones with a genistein:daidzein ratio of 1:4 [Rottapharm (RPH)-aglycone] was examined for its effects on the repair of DNA damage induced by a single dose of UVB irradiation (20 mJ/cm(2)). For this purpose, human skin cells were first UVB-irradiated and then treated with RPH-aglycone. Comet assay analysis was used to estimate the UVB-induced DNA damage at different time points after treatment by measuring the tail moment parameter. We found that treatment with 10 μmol/L RPH-aglycone solution resulted in a significantly reduced tail moment at 1h after treatment, and 34-35% enhancement of damage repair at 4 h after treatment. These results suggest that isoflavone aglycones are protective against UVB-induced DNA damage.
authors.affiliation: Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, Naples, Italy.
authors.forename: B
authors.initials: B
authors.lastname: Iovine
authors.forename: M
authors.initials: M
authors.lastname: Garofalo
authors.forename: M
authors.initials: M
authors.lastname: Orefice
authors.forename: V
authors.initials: V
authors.lastname: Giannini
authors.forename: F
authors.initials: F
authors.lastname: Gasparri
authors.forename: G
authors.initials: G
authors.lastname: Monfrecola
authors.forename: M A
authors.initials: MA
authors.lastname: Bevilacqua
mesh_terms: D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects*; D000078404:Epidermal Cells; D004847:Epithelial Cells / Q000187:drug effects / Q000528:radiation effects; D019833:Genistein / Q000494:pharmacology*; D006801:Humans; D007529:Isoflavones / Q000494:pharmacology*; D015595:Skin Aging / Q000187:drug effects; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D016588:Anticarcinogenic Agents; D007529:Isoflavones; C004742:daidzein; D019833:Genistein