title: [Study on realgar nanoparticles inhibition of adenovirus replication at the gene level].
abstract: OBJECTIVE
  Modeling HAdV-3 infect Hep-2 cells in vitro. The effect of realgar nanoparticles on the expression of HAdV-3 is detected by using fluorescent quantitative PCR.
  
  
  METHODS
  The experiment is divided into four groups: Hep-2 cells control group, HAdV-3 virus control group, realgar nanoparticle group and ribavirin group. In order to detect HAdV-3 viral load, add realgar nanoparticles and ribavirin in vitro and remain that vitro for 24 hours when HAdV-3 has infected Hep-2 cells, extract total DNA of Hep-2 cells infected by HAdV-3, and establish Real-time PCR reaction system of every experimental groups.
  
  
  RESULTS
  The Hep-2 cells group has no amplification curve, the Ct value is greater than 35, which illustrate HAdV-3 pathogen detection is negative. However, realgar nanoparticles group, ribavirin group and the HAdV-3 group have amplification curve, the Ct values are 29.30 +/- 0.08, 33.05 +/- 1.29, 26.01 +/- 0.25 respectively, which illustrate HAdV-3 pathogen detection is positive. The viral copy amount of the adenovirus group(66 699 932 +/- 23.85) is more than that of realgar nanoparticles group (912 435.44 +/- 16.57), and much greater than that of ribavirin group (459 124.84 +/- 12.82) (P < 0.05).
  
  
  CONCLUSIONS
  The model of Hep-2 cell infected by HAdV-3 is reliable. The method of quantitative PCR is sensitive and specific. Realgar nanoparticles have a certain inhibition role for adenovirus nucleic acid replication.
authors.forename: Ming-Zhe
authors.initials: MZ
authors.lastname: Wang
authors.forename: Wushouer
authors.initials: W
authors.lastname: Fuerhati
authors.forename: Cheng-Xiang
authors.initials: CX
authors.lastname: Wang
authors.forename: Wen-Bo
authors.initials: WB
authors.lastname: Xu
mesh_terms: D000257:Adenoviridae Infections / Q000821:virology*; D000260:Adenoviruses, Human / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D001152:Arsenicals / Q000737:chemistry / Q000494:pharmacology*; D015967:Gene Expression Regulation, Viral / Q000187:drug effects*; D056945:Hep G2 Cells; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry; D013440:Sulfides / Q000737:chemistry / Q000494:pharmacology*; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D001152:Arsenicals; D013440:Sulfides; C058317:arsenic disulfide