title: Dual role for CHOP in the crosstalk between autophagy and apoptosis to determine cell fate in response to amino acid deprivation.
abstract: CHOP encodes a ubiquitous transcription factor that is one of the most important components in the network of stress-inducible transcription. In particular, this factor is known to mediate cell death in response to stress. The focus of this work is to study its pivotal role in the control of cell viability according to the duration of a stress like amino acid starvation. We show that during the first 6h of starvation, CHOP upregulates a number of autophagy genes but is not involved in the first steps of the autophagic process. By contrast, when the amino acid starvation is prolonged (16-48h), we demonstrated that CHOP has a dual role in both inducing apoptosis and limiting autophagy through the transcriptional control of specific target genes. Overall, this study reveals a novel regulatory role for CHOP in the crosstalk between autophagy and apoptosis in response to stress.
authors.affiliation: INRA, UMR 1019 Nutrition Humaine, Centre de Clermont-Ferrand-Theix, F-63122 Saint Genès Champanelle, France; Université Clermont 1, UFR Médecine, UMR 1019 Nutrition Humaine, Clermont-Ferrand, France.
authors.forename: Wafa
authors.initials: W
authors.lastname: B'chir
authors.affiliation: INRA, UMR 1019 Nutrition Humaine, Centre de Clermont-Ferrand-Theix, F-63122 Saint Genès Champanelle, France; Université Clermont 1, UFR Médecine, UMR 1019 Nutrition Humaine, Clermont-Ferrand, France.
authors.forename: Cédric
authors.initials: C
authors.lastname: Chaveroux
authors.affiliation: INRA, UMR 1019 Nutrition Humaine, Centre de Clermont-Ferrand-Theix, F-63122 Saint Genès Champanelle, France; Université Clermont 1, UFR Médecine, UMR 1019 Nutrition Humaine, Clermont-Ferrand, France.
authors.forename: Valérie
authors.initials: V
authors.lastname: Carraro
authors.affiliation: INRA, UMR 1019 Nutrition Humaine, Centre de Clermont-Ferrand-Theix, F-63122 Saint Genès Champanelle, France; Université Clermont 1, UFR Médecine, UMR 1019 Nutrition Humaine, Clermont-Ferrand, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Averous
authors.affiliation: INRA, UMR 1019 Nutrition Humaine, Centre de Clermont-Ferrand-Theix, F-63122 Saint Genès Champanelle, France; Université Clermont 1, UFR Médecine, UMR 1019 Nutrition Humaine, Clermont-Ferrand, France.
authors.forename: Anne-Catherine
authors.initials: AC
authors.lastname: Maurin
authors.affiliation: INRA, UMR 1019 Nutrition Humaine, Centre de Clermont-Ferrand-Theix, F-63122 Saint Genès Champanelle, France; Université Clermont 1, UFR Médecine, UMR 1019 Nutrition Humaine, Clermont-Ferrand, France.
authors.forename: Céline
authors.initials: C
authors.lastname: Jousse
authors.affiliation: INRA, UMR 1019 Nutrition Humaine, Centre de Clermont-Ferrand-Theix, F-63122 Saint Genès Champanelle, France; Université Clermont 1, UFR Médecine, UMR 1019 Nutrition Humaine, Clermont-Ferrand, France.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Muranishi
authors.affiliation: INRA, UMR 1019 Nutrition Humaine, Centre de Clermont-Ferrand-Theix, F-63122 Saint Genès Champanelle, France; Université Clermont 1, UFR Médecine, UMR 1019 Nutrition Humaine, Clermont-Ferrand, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Parry
authors.affiliation: INRA, UMR 1019 Nutrition Humaine, Centre de Clermont-Ferrand-Theix, F-63122 Saint Genès Champanelle, France; Université Clermont 1, UFR Médecine, UMR 1019 Nutrition Humaine, Clermont-Ferrand, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Fafournoux
authors.affiliation: INRA, UMR 1019 Nutrition Humaine, Centre de Clermont-Ferrand-Theix, F-63122 Saint Genès Champanelle, France; Université Clermont 1, UFR Médecine, UMR 1019 Nutrition Humaine, Clermont-Ferrand, France. Electronic address: alain.bruhat@clermont.inra.fr.
authors.forename: Alain
authors.initials: A
authors.lastname: Bruhat
mesh_terms: D000596:Amino Acids / Q000172:deficiency*; D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D001343:Autophagy / Q000235:genetics*; D002460:Cell Line; D002470:Cell Survival / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D008164:Luminescent Proteins / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D013217:Starvation; D013312:Stress, Physiological; D051742:Transcription Factor CHOP / Q000235:genetics*; D000097573:Red Fluorescent Protein
chemical_list: D000596:Amino Acids; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D008869:Microtubule-Associated Proteins; D012333:RNA, Messenger; D051742:Transcription Factor CHOP; D000097573:Red Fluorescent Protein; C497250:Ddit3 protein, mouse; C480860:Map1lc3b protein, mouse; C485184:enhanced green fluorescent protein
keywords: Amino acid starvation; Apoptosis; Autophagy; CHOP; Gene transcription