title: Direct conversion of pretreated straw cellulose into citric acid by co-cultures of Yarrowia lipolytica SWJ-1b and immobilized Trichoderma reesei mycelium.
abstract: The immobilized cellulase-producing mycelium of Trichoderma reesei was found to produce 2.9 U/ml of cellulase activity within 144 h while 2.1 U/ml of cellulase activity was produced within 120 h by the free mycelium of the same strain. When the immobilized mycelium of T. reesei was co-cultivated with the free cells of Yarrowia lipolytica SWJ-1b in flask, Y. lipolytica SWJ-1b could yield 10.7 g/l of citric acid and 3.9 g/l of isocitric acid from 40.0 g/l pretreated straw within 240 h. Under the similar conditions, Y. lipolytica SWJ-1b could yield 32.8 g/l of citric acid and 4.7 g/l of isocitric acid from 40.0 g/l pretreated straw supplemented with 20.0 g/l glucose within 288 h. When the co-cultures were grown in 10-l fermentor, Y. lipolytica SWJ-1b could yield 83.4 g/l of citric acid and 8.7 g/l of isocitric acid from 100.0 g/l of pretreated straw supplemented with 50.0 g/l glucose within 312 h.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Liu
authors.forename: Jinshun
authors.initials: J
authors.lastname: Lv
authors.forename: Tong
authors.initials: T
authors.lastname: Zhang
authors.forename: Yuanfang
authors.initials: Y
authors.lastname: Deng
mesh_terms: D018914:Cells, Immobilized / Q000378:metabolism; D002480:Cellulase / Q000096:biosynthesis / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism*; D019343:Citric Acid / Q000378:metabolism*; D018920:Coculture Techniques; D005285:Fermentation; D025282:Mycelium / Q000166:cytology / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D013172:Spores, Fungal / Q000166:cytology / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism; D014242:Trichoderma / Q000166:cytology / Q000201:enzymology / Q000254:growth & development* / Q000378:metabolism*; D025062:Yarrowia / Q000254:growth & development* / Q000378:metabolism*
chemical_list: D019343:Citric Acid; D002482:Cellulose; D002480:Cellulase