title: Impact of STAT/SOCS mRNA expression levels after major injury.
abstract: BACKGROUND
  Fulminant changes in cytokine receptor signalling might provoke severe pathological alterations after multiple trauma. The aim of this study was to evaluate the posttraumatic imbalance of the innate immune system with a special focus on the STAT/SOCS family.
  
  
  METHODS
  20 polytraumatized patients were included. Blood samples were drawn 0 h-72 h after trauma; mRNA expression profiles of IL-10, STAT 3, SOCS 1, and SOCS 3 were quantified by qPCR.
  
  
  RESULTS
  IL-10 mRNA expression increased significantly in the early posttraumatic period. STAT 3 mRNA expressions showed a significant maximum at 6 h after trauma. SOCS 1 levels significantly decreased 6 h-72 h after trauma. SOCS 3 levels were significantly higher in nonsurvivors 6 h after trauma.
  
  
  CONCLUSIONS
  We present a serial, sequential investigation in human neutrophil granulocytes of major trauma patients evaluating mRNA expression profiles of IL-10, STAT 3, SOCS 1, and SOCS 3. Posttraumatically, immune disorder was accompanied by a significant increase of IL-10 and STAT 3 mRNA expression, whereas SOCS 1 mRNA levels decreased after injury. We could demonstrate that death after trauma was associated with higher SOCS 3 mRNA levels already at 6 h after trauma. To support our results, further investigations have to evaluate protein levels of STAT/SOCS family in terms of posttraumatic immune imbalance.
authors.affiliation: Department of Trauma Surgery, Ludwig Maximilians University Hospital Munich, Nußbaumstraße 20, 80336 Munich, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Brumann
authors.affiliation: Department of Trauma Surgery, Ludwig Maximilians University Hospital Munich, Nußbaumstraße 20, 80336 Munich, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Matz
authors.affiliation: Department of Trauma Surgery, Ludwig Maximilians University Hospital Munich, Nußbaumstraße 20, 80336 Munich, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Kusmenkov
authors.affiliation: Department of Trauma Surgery, Ludwig Maximilians University Hospital Munich, Nußbaumstraße 20, 80336 Munich, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Stegmaier
authors.affiliation: Department of Trauma Surgery, Ludwig Maximilians University Hospital Munich, Nußbaumstraße 20, 80336 Munich, Germany.
authors.forename: P
authors.initials: P
authors.lastname: Biberthaler
authors.affiliation: Department of Trauma Surgery, Ludwig Maximilians University Hospital Munich, Nußbaumstraße 20, 80336 Munich, Germany.
authors.forename: K-G
authors.initials: KG
authors.lastname: Kanz
authors.affiliation: Department of Trauma Surgery, Ludwig Maximilians University Hospital Munich, Nußbaumstraße 20, 80336 Munich, Germany.
authors.forename: W
authors.initials: W
authors.lastname: Mutschler
authors.affiliation: Department of Trauma Surgery, Ludwig Maximilians University Hospital Munich, Nußbaumstraße 20, 80336 Munich, Germany.
authors.forename: V
authors.initials: V
authors.lastname: Bogner
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006098:Granulocytes / Q000166:cytology; D006801:Humans; D007113:Immunity, Innate; D016753:Interleukin-10 / Q000097:blood; D008297:Male; D008875:Middle Aged; D009104:Multiple Trauma / Q000097:blood*; D012333:RNA, Messenger / Q000378:metabolism; D050796:STAT3 Transcription Factor / Q000097:blood*; D000071222:Suppressor of Cytokine Signaling 1 Protein; D000071223:Suppressor of Cytokine Signaling 3 Protein; D050826:Suppressor of Cytokine Signaling Proteins / Q000097:blood* / Q000378:metabolism*; D013997:Time Factors; D055815:Young Adult
chemical_list: C508609:IL10 protein, human; D012333:RNA, Messenger; C108944:SOCS1 protein, human; C106644:SOCS3 protein, human; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; D000071222:Suppressor of Cytokine Signaling 1 Protein; D000071223:Suppressor of Cytokine Signaling 3 Protein; D050826:Suppressor of Cytokine Signaling Proteins; D016753:Interleukin-10