title: Effect of seeds of Achyranthes aspera on the immune responses and expression of some immune-related genes in carp Catla catla.
abstract: Evaluation of functional mechanism of immunostimulant in fish is essential for realizing their therapeutic potential. The impact of dietary supplementation of seeds of Achyranthes aspera on the immune system of Catla catla was studied. Catla (37.73 ± 6.7 g) were fed with test diets containing 0.5 (D1) and 1.0% (D2) seeds of A. aspera and control diet (D3) for 50 days. Then fish were immunized with heat-killed Aeromonas hydrophila. Blood and tissue samples were collected after 7 days of immunization. Three fish were sacrificed for each parameter. Respiratory burst activity, bactericidal activity and antibody response were significantly (P < 0.05) higher in test diets fed catla compared to the control one. Nitric oxide synthase and serum lysozyme were significantly (P < 0.05) higher in fish fed with diet D2 compared to the other treatments. There were up-regulations of TNFα, lysozyme c and g gene expressions in kidney and IL-10, lysozyme c and g expressions in hepatopancreas of test diets fed fish compared to the control one. The expression of IL-10 was down-regulated in the kidney of D2 diet fed fish compared to others. In the gill, significantly (P < 0.05) higher expressions of TNFα was found in D1 diet feed fish and lysozyme c and in D2 diet fed fish compared to the other groups. This study showed tissue-specific gene expression pattern in catla. An inverse relationship was found between the expression of TNFα and IL-10 in kidney of catla.
authors.affiliation: Aqua Research Lab, Department of Zoology, University of Delhi, Delhi 110007, India. Electronic address: aquaresearchlab@yahoo.co.in.
authors.forename: Rina
authors.initials: R
authors.lastname: Chakrabarti
authors.affiliation: Aqua Research Lab, Department of Zoology, University of Delhi, Delhi 110007, India.
authors.forename: Praveen Kumar
authors.initials: PK
authors.lastname: Srivastava
authors.affiliation: Aqua Research Lab, Department of Zoology, University of Delhi, Delhi 110007, India.
authors.forename: Nandini
authors.initials: N
authors.lastname: Verma
authors.affiliation: Department of Biotechnology, Delhi Technological University, Delhi 110042, India.
authors.forename: JaiGopal
authors.initials: J
authors.lastname: Sharma
mesh_terms: D029044:Achyranthes / Q000276:immunology*; D016980:Aeromonas hydrophila / Q000276:immunology*; D000818:Animals; D017756:Aquaculture; D002347:Carps; D005393:Fish Diseases / Q000097:blood / Q000235:genetics / Q000276:immunology / Q000382:microbiology*; D005786:Gene Expression Regulation / Q000276:immunology*; D005880:Gills / Q000276:immunology; D016905:Gram-Negative Bacterial Infections / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D043143:Hepatopancreas / Q000276:immunology; D056747:Immunomodulation / Q000276:immunology; D016753:Interleukin-10 / Q000097:blood; D007668:Kidney / Q000276:immunology; D009113:Muramidase / Q000097:blood; D019001:Nitric Oxide Synthase / Q000097:blood; D011897:Random Allocation; D016897:Respiratory Burst / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D019001:Nitric Oxide Synthase; D009113:Muramidase
keywords: Antibody response; Catla; Gene expression; Immunostimulant; Lysozyme