title: Efficient hit and lead compound evaluation strategy based on off-rate screening by surface plasmon resonance.
abstract: The time-consuming and labor intensive compound separation and purification process before they can be evaluated have been the bottleneck steps of novel drug discovery. A new screening strategy that can evaluate crude reaction mixtures without separation and purification by determining dissociation rate constants of compounds in crude reaction mixtures was developed and validated by scientists from the Vernalis. This new strategy will significantly improve the efficiency of early stages in drug discovery.
authors.affiliation: Comprehensive Cancer Center and Department of Internal Medicine, University of Michigan , 1500 E. Medical Center Drive, Ann Arbor, Michigan 48109, United States.
authors.forename: Liu
authors.initials: L
authors.lastname: Liu
mesh_terms: D015374:Biosensing Techniques; D055808:Drug Discovery; D004353:Drug Evaluation, Preclinical; D006801:Humans; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D054852:Small Molecule Libraries / Q000737:chemistry*; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D004364:Pharmaceutical Preparations; D054852:Small Molecule Libraries