title: Bcl-2 knockdown accelerates T cell receptor-triggered activation-induced cell death in jurkat T cells.
abstract: Cell death and survival are tightly controlled through the highly coordinated activation/inhibition of diverse signal transduction pathways to insure normal development and physiology. Imbalance between cell death and survival often leads to autoimmune diseases and cancer. Death receptors sense extracellular signals to induce caspase-mediated apoptosis. Acting upstream of CED-3 family proteases, such as caspase-3, Bcl-2 prevents apoptosis. Using short hairpin RNAs (shRNAs), we suppressed Bcl-2 expression in Jurkat T cells, and this increased TCR-triggered AICD and enhanced TNFR gene expression. Also, knockdown of Bcl-2 in Jurkat T cells suppressed the gene expression of FLIP, TNF receptor-associated factors 3 (TRAF3) and TRAF4. Furthermore, suppressed Bcl-2 expression increased caspase-3 and diminished nuclear factor kappa B (NF-κB) translocation.
authors.affiliation: Laboratory of Host Defense Modulation, College of Pharmacy, Chung-Ang University, Seoul 156-756, Korea.
authors.forename: Yun-Jung
authors.initials: YJ
authors.lastname: Lee
authors.affiliation: Laboratory of Host Defense Modulation, College of Pharmacy, Chung-Ang University, Seoul 156-756, Korea.
authors.forename: Tae Joon
authors.initials: TJ
authors.lastname: Won
authors.affiliation: Laboratory of Host Defense Modulation, College of Pharmacy, Chung-Ang University, Seoul 156-756, Korea.
authors.forename: Kyeong Eun
authors.initials: KE
authors.lastname: Hyung
authors.affiliation: Laboratory of Host Defense Modulation, College of Pharmacy, Chung-Ang University, Seoul 156-756, Korea.
authors.forename: Mi Ji
authors.initials: MJ
authors.lastname: Lee
authors.affiliation: Laboratory of Host Defense Modulation, College of Pharmacy, Chung-Ang University, Seoul 156-756, Korea.
authors.forename: Young-Hye
authors.initials: YH
authors.lastname: Moon
authors.affiliation: Laboratory of Host Defense Modulation, College of Pharmacy, Chung-Ang University, Seoul 156-756, Korea.
authors.forename: Ik Hee
authors.initials: IH
authors.lastname: Lee
authors.affiliation: Laboratory of Host Defense Modulation, College of Pharmacy, Chung-Ang University, Seoul 156-756, Korea.
authors.forename: Byung Sung
authors.initials: BS
authors.lastname: Go
authors.affiliation: Laboratory of Host Defense Modulation, College of Pharmacy, Chung-Ang University, Seoul 156-756, Korea.
authors.forename: Kwang Woo
authors.initials: KW
authors.lastname: Hwang
keywords: AICD; Apoptosis; Bcl-2; T cell; shRNA