title: Detection of adenosine 5'-triphosphate by fluorescence variation of oligonucleotide-templated silver nanoclusters.
abstract: Oligonucleotide-templated Ag nanoclusters (DNA-Ag NCs) prepared from AgNO3 using an oligonucleotide (5'-TAACCCCTAACCCCT-3') as a template and NaBH4 as a reducing agent have been used for sensing of adenosine 5'-triphosphate (ATP). The fluorescence intensity and emission wavelength of DNA-Ag NCs are dependent on the pH value and ATP concentration. At pH 3.0 and 11.0, ATP shows greater effects on fluorescence of the DNA-Ag NCs. Upon increasing ATP concentration from 10 to 50μM, their emission wavelength at pH 3.0 shifts from 525 to 585nm. At pH 11.0, their fluorescence intensity (510nm) increases upon increasing ATP concentration. The circular dichroism (CD), electrospray ionization-mass spectrometry (ESI-MS), absorption, and fluorescence results indicate that ATP and pH affect the interactions between DNAs and Ag atoms, resulting in changes in their fluorescence. The DNA-Ag NCs allow detection of ATP over a concentration range of 0.1-10μM, with a limit of detection 33nM. Practicality of the DNA-Ag NCs probe has been validated with the determination of ATP concentrations in the lysate of MDA-MB-231 breast carcinoma cells.
authors.affiliation: Department of Chemistry, National Taiwan University, 1, Section 4, Roosevelt Road, Taipei 10617, Taiwan.
authors.forename: Jennifer Daneen
authors.initials: JD
authors.lastname: Lee
authors.affiliation: Department of Chemical Engineering, Yancheng Institute of Industry Technology, Yancheng, Jiangsu, China.
authors.forename: Jinshun
authors.initials: J
authors.lastname: Cang
authors.affiliation: Department of Chemistry, National Taiwan University, 1, Section 4, Roosevelt Road, Taipei 10617, Taiwan.
authors.forename: Ying-Chieh
authors.initials: YC
authors.lastname: Chen
authors.affiliation: Department of Chemistry, National Taiwan University, 1, Section 4, Roosevelt Road, Taipei 10617, Taiwan.
authors.forename: Wei-Yu
authors.initials: WY
authors.lastname: Chen
authors.affiliation: Department of Chemistry, National Taiwan University, 1, Section 4, Roosevelt Road, Taipei 10617, Taiwan.
authors.forename: Chung-Mao
authors.initials: CM
authors.lastname: Ou
authors.affiliation: Department of Chemistry, National Taiwan University, 1, Section 4, Roosevelt Road, Taipei 10617, Taiwan. Electronic address: changht@ntu.edu.tw.
authors.forename: Huan-Tsung
authors.initials: HT
authors.lastname: Chang
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D015374:Biosensing Techniques / Q000295:instrumentation; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D004867:Equipment Design; D019544:Equipment Failure Analysis; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D054802:Molecular Imprinting / Q000295:instrumentation / Q000379:methods*; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000295:instrumentation; D012834:Silver / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000295:instrumentation*
chemical_list: D012834:Silver; D000255:Adenosine Triphosphate; D004247:DNA
keywords: Adenosine 5′-triphosphate; Ag nanoclusters; DNA structure; Fluorescence