title: Foetal life protein provision of mink (Neovison vison) changes the relative mRNA abundance of some hepatic enzymes regulating fat metabolism.
abstract: The nutrient provision to pregnant females has high impact on the growth and metabolism of their offspring. The objective was to investigate if the expression of hepatic enzymes regulating the fat metabolism was affected in foetuses and adult female mink born by dams fed either a low or an adequate level of protein during late gestation. The relative abundances of acetyl coenzyme A carboxylase (ACC), fatty acid synthase (FAS) and carnitine palmitoyl transferase 1 (CPT1) mRNA were determined by qualitative polymerase chain reaction in the livers of F₀- and F₁-generation dams and in F₁-generation foetuses. Low protein provision during foetal life resulted in a lower expression of FAS in foetal liver but a tendency towards increased expression in the liver of adult dams. There was a tendency towards an effect of life stage of the animal on the expression of ACC resulting in a higher expression among F₁ foetuses exposed to low protein during foetal life than F₀ dams fed a low protein diet during late gestation. The expression of CPT1 was significantly lower among dams exposed to low protein provision during foetal life than controls, possibly indicating a lower rate of mitochondrial β-oxidation. Further investigations are needed to clarify the consequences of these changes for the fat metabolism.
authors.affiliation: a Department of Veterinary Clinical and Animal Sciences , Faculty of Health and Medical Sciences, University of Copenhagen , Copenhagen , Denmark.
authors.forename: Connie Frank
authors.initials: CF
authors.lastname: Matthiesen
authors.forename: Maria Arantzazu Aguinaga
authors.initials: MA
authors.lastname: Casañas
authors.forename: Anne-Helene
authors.initials: AH
authors.lastname: Tauson
mesh_terms: D000103:Acetyl-CoA Carboxylase / Q000235:genetics / Q000378:metabolism; D000821:Animal Feed / Q000032:analysis; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D002334:Carnitine O-Palmitoyltransferase / Q000235:genetics / Q000378:metabolism; D018753:Diet, Protein-Restricted / Q000662:veterinary*; D004044:Dietary Proteins / Q000378:metabolism; D005223:Fats / Q000378:metabolism*; D064429:Fatty Acid Synthases / Q000235:genetics / Q000378:metabolism; D005260:Female; D005333:Fetus / Q000502:physiology; D005786:Gene Expression Regulation; D008099:Liver / Q000201:enzymology*; D008907:Mink / Q000196:embryology / Q000235:genetics* / Q000378:metabolism; D009751:Nutritional Requirements; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D004044:Dietary Proteins; D005223:Fats; D012333:RNA, Messenger; D002334:Carnitine O-Palmitoyltransferase; D064429:Fatty Acid Synthases; D000103:Acetyl-CoA Carboxylase