title: Analysis of thiopurines using aqueous normal phase chromatography.
abstract: The chromatography of several thiopurines is investigated using aqueous normal phase (ANP) conditions in conjunction with a silica hydride-based column. Both isocratic and gradient elution modes are tested. Detection of higher concentration samples is done by UV to demonstrate feasibility in this format while lower concentration samples utilize mass spectrometry (MS). Repeatability of successive runs is also tested with particular attention to gradient methods where the equilibration time of the stationary phase can be evaluated.
authors.affiliation: Department of Chemistry, San Jose State University, San Jose, CA 95112, USA. Electronic address: pesek@sjsu.edu.
authors.forename: Joseph J
authors.initials: JJ
authors.lastname: Pesek
authors.affiliation: Department of Chemistry, San Jose State University, San Jose, CA 95112, USA.
authors.forename: Maria T
authors.initials: MT
authors.lastname: Matyska
authors.affiliation: MicroSolv Technology Corporation, One Industrial Way West, Eatontown, NJ 01776, USA.
authors.forename: Joshua E
authors.initials: JE
authors.lastname: Young
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006150:Guanine Nucleotides / Q000032:analysis; D006151:Guanosine / Q000031:analogs & derivatives / Q000032:analysis; D013866:Thioguanine / Q000032:analysis*; D013872:Thionucleosides / Q000032:analysis*; D013873:Thionucleotides / Q000032:analysis*
chemical_list: D006150:Guanine Nucleotides; D013872:Thionucleosides; D013873:Thionucleotides; D006151:Guanosine; C003964:6-thioguanylic acid; C005783:6-thioguanosine; D013866:Thioguanine
keywords: Hydrophilic analytes; Metabolites; Pharmaceutical analysis; Silica hydride stationary phase