title: Comparative study of various growth factors and cytokines on type I collagen and hyaluronan production in human dermal fibroblasts.
abstract: BACKGROUND
  Dermal fibroblast is a primary cell type responsible for synthesis and remodeling of extracellular matrix in human skin. Type I collagen and hyaluronan are main components that have roles in skin fibrosis, wound healing, tissue remodeling as well as skin aging. Several studies have reported cytokine-dependent changes in collagen expression or hyaluronan production; however, the cytokines' effect was controversial in human dermal fibroblasts.
  
  
  OBJECTIVE
  To clarify the role of various growth factors, cytokines or chemokines on the production of interstitial type I collagen and hyaluronan in dermal fibroblasts.
  
  
  METHODS
  We confirmed the presence of various corresponding receptors and assessed the effects of 33 human recombinants on the production of type I collagen and hyaluronan using the assay system in dermal fibroblasts.
  
  
  RESULTS
  Platelet-derived growth factor (PDGF)-AA, PDGF-BB, epidermal growth factor (EGF), transforming growth factor (TGF)-β1, MCP-1, IP-10, interleukin (IL)-1α, IL-1β, and IL-15 were effective on both type I collagen and hyaluronan production, as compared with no stimulated control. On the other hand, IL-10 and IFN- α caused a significant decrease in type I collagen production, and IL-8 and GM-CSF caused a decrease in hyaluronan production compared with no cytokine-treated control. Interestingly, some chemokines, such as MCP-1 (CCL2), RANTES (CCL5), eotaxin-2 (CCL24), IP-10 (CXCL10), or fractalkine (CX3CL1) significantly induced the type I collagen or hyaluronan production.
  
  
  CONCLUSIONS
  Various growth factors and cytokines on the regulation of type I collagen and hyaluronan in human dermal skin probably function as key factors in skin remodeling and skin aging. Our profile may help to apply to cosmeceutical area maintaining as young skin through the increase in extracellular matrix.
authors.affiliation: R&D Center, LG Household & Healthcare Ltd., Daejeon, Korea.
authors.forename: Mi-Sun
authors.initials: MS
authors.lastname: Kim
authors.forename: Hye Jin
authors.initials: HJ
authors.lastname: Song
authors.forename: Sang Hwa
authors.initials: SH
authors.lastname: Lee
authors.forename: Cheon Koo
authors.initials: CK
authors.lastname: Lee
mesh_terms: D000077214:Becaplermin; D024042:Collagen Type I / Q000096:biosynthesis*; D016207:Cytokines / Q000494:pharmacology; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism*; D006801:Humans; D006820:Hyaluronic Acid / Q000096:biosynthesis*; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology*; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D020574:Proto-Oncogene Proteins c-sis / Q000494:pharmacology; D011994:Recombinant Proteins / Q000494:pharmacology
chemical_list: D024042:Collagen Type I; D016207:Cytokines; D036341:Intercellular Signaling Peptides and Proteins; D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D011994:Recombinant Proteins; D000077214:Becaplermin; D004815:Epidermal Growth Factor; D006820:Hyaluronic Acid
keywords: collagen; cytokine/chemokine; dermal fibroblast; growth factor; hyaluronan