title: High-resolution mass spectrometry to evaluate the influence of cross-breeding segregating populations on the phenolic profile of virgin olive oils.
abstract: BACKGROUND
  The growing demand for high-quality virgin olive oils (VOOs) has increased the interest in olive breeding programs. Cross-breeding is considered, within these programs, the best strategy to generate new cultivars as an attempt to improve the present cultivars. In this research, the phenolic profile of VOOs from target crosses (Arbequina × Arbosana, Picual × Koroneiki and Sikitita × Arbosana) and their corresponding genitors (Arbequina, Arbosana, Koroneiki, Picual and Sikitita) has been evaluated using a targeted metabolomics approach.
  
  
  RESULTS
  The phenolic profiles were obtained by liquid chromatographic-hybrid quadrupole time-of-flight mass spectrometric targeted analysis of 37 phenols or compounds involved in the main pathways for their biosynthesis. Statistical multivariate analysis by principal component analysis was applied to study the influence of genotype on phenol composition. Phenolic compounds with the highest contribution to explain the observed variability associated to genotype were identified through fold change algorithms (cut-off > 2.0) and t-test analysis.
  
  
  CONCLUSIONS
  A total of nine phenols (viz. quercetin, ligstroside aglycon (p-HPEA-EA), demethyl oleuropein aglycon, oleuropein aglycon (3,4-DHPEA-EA), hydroxypinoresinol, hydroxytyrosol and phenolic acids such as p-coumaric acid, ferulic acid and protocatechuic acid) contributed to explain the observed variability with 99% confidence (P<0.01).
authors.affiliation: Department of Analytical Chemistry, Campus of Rabanales, University of Córdoba, Córdoba, Spain; University of Córdoba Agroalimentary Excellence Campus, Campus of Rabanales, 14071, Córdoba, Spain; Maimónides Institute for Research in Biomedicine of Cordoba, Reina Sofia University Hospital, University of Córdoba, Córdoba, Spain.
authors.forename: Verónica
authors.initials: V
authors.lastname: Sánchez de Medina
authors.forename: Mónica
authors.initials: M
authors.lastname: Calderón-Santiago
authors.forename: Milad
authors.initials: M
authors.lastname: El Riachy
authors.forename: Feliciano
authors.initials: F
authors.lastname: Priego-Capote
authors.forename: María Dolores
authors.initials: MD
authors.lastname: Luque de Castro
mesh_terms: D002853:Chromatography, Liquid; D005838:Genotype; D006824:Hybridization, Genetic; D013058:Mass Spectrometry / Q000379:methods*; D055432:Metabolomics / Q000379:methods; D015999:Multivariate Analysis; D031658:Olea / Q000235:genetics*; D000069463:Olive Oil; D010636:Phenols / Q000032:analysis*; D010938:Plant Oils / Q000737:chemistry*
chemical_list: D000069463:Olive Oil; D010636:Phenols; D010938:Plant Oils
keywords: LC-QqTOF analysis; cross-breeding; cultivar; phenols; segregating populations; virgin olive oil