title: Genetically modified microorganism Spingomonas paucimobilis UT26 for simultaneously degradation of methyl-parathion and γ-hexachlorocyclohexane.
abstract: Bioremediation of pesticide residues by bacteria is an efficient and environmentally friendly method to deal with environmental pollution. In this study, a genetically modified microorganism (GMM) named UT26XEGM was constructed by introducing a parathion hydrolase gene into an initially γ-hexachlorocyclohexane (γ-HCH) degrading bacterium Spingomonas paucimobilis UT26. In order to reduce its potential risk of gene escaping into the environment for the public concern on biosafety, a suicide system was also designed that did not interfere with the performance of the GMM until its physiological function was activated by specific signal. The system was designed with circuiting suicide cassettes consisting of killing genes gef and ecoRIR from Escherichia coli controlled by Pm promoter and the xylS gene. The cell viability and original degradation characteristics were not affected by the insertion of exogenous genes. The novel GMM was capable of degrading methyl-parathion and γ-HCH simultaneously. In laboratory scale testing, the recombinant bacteria were successfully applied to the bioremediation of mixed pesticide residues with the activity of self-destruction after 3-methylbenzoate induction.
authors.affiliation: Shenzhen R&D Key Laboratory of Alien Pest Detection Technology, Animal & Plant Inspection and Quarantine Technical Center, Shenzhen Entry-Exit Inspection and Quarantine Bureau, 1011 Fuqiang Road, Shenzhen, 518045, People's Republic of China, lanwshao@163.com.
authors.forename: Wen S
authors.initials: WS
authors.lastname: Lan
authors.forename: Ti K
authors.initials: TK
authors.lastname: Lu
authors.forename: Zhi F
authors.initials: ZF
authors.lastname: Qin
authors.forename: Xiu J
authors.initials: XJ
authors.lastname: Shi
authors.forename: Jin J
authors.initials: JJ
authors.lastname: Wang
authors.forename: Yun F
authors.initials: YF
authors.lastname: Hu
authors.forename: Bin
authors.initials: B
authors.lastname: Chen
authors.forename: Yi H
authors.initials: YH
authors.lastname: Zhu
authors.forename: Zheng
authors.initials: Z
authors.lastname: Liu
mesh_terms: D001673:Biodegradation, Environmental; D004785:Environmental Pollutants / Q000378:metabolism; D041722:Genes, Transgenic, Suicide; D001556:Hexachlorocyclohexane / Q000378:metabolism*; D030781:Organisms, Genetically Modified; D010278:Parathion / Q000378:metabolism*; D020578:Sphingomonas / Q000235:genetics / Q000378:metabolism*
chemical_list: D004785:Environmental Pollutants; D001556:Hexachlorocyclohexane; D010278:Parathion