title: Development of biodegradable antibacterial cellulose based hydrogel membranes for wound healing.
abstract: Cellulose-based hydrogels have wide applications in tissue engineering and controlled delivery systems. In this study, chloramphenicol (CAP) loaded 2,3 dialdehyde cellulose (DABC) hydrogel membranes were prepared, characterized and their antibacterial efficacy was evaluated. Bacterial cellulose (BC) secreted by Acetobacter xylinum was modified to become DABC by oxidation via the sodium metaperiodate method. CAP-BC and CAP-DABC interactions were illustrated via ATR-FTIR analysis. Water retention capacity of BC and DABC membranes were determined as 65.6±1.6% and 5.3±0.3%, respectively. CAP release profiles were determined via HPLC analysis. The drug-loading capacities of BC and DABC membranes were 5mg/cm(2) and 0.1mg/cm(2), respectively. Membranes released 99-99.5% of the contained CAP within 24h and an initial burst release effect was not observed. In vitro antibacterial tests of BC and DABC, both CAP-loaded, demonstrated their ability to inhibit bacterial growth for a prolonged duration. Antimicrobial effect against bacteria was still prevalent after 3 days of incubation period with disc diffusion tests. The MTT test results reveal that fibroblast adhesion and proliferation on CAP-loaded DABC membranes were noticeably higher than CAP-loaded BC membrane. This newly developed drug containing DABC membranes seem to be highly suitable for wound healing due to its unique properties of biodegradability, biocompatibility, and antimicrobial effectiveness.
authors.affiliation: Science and Technology Application and Research Center, Yıldız Technical University, 34349 Istanbul, Turkey. Electronic address: melisalacin@yahoo.com.
authors.forename: Nelisa Türkoğlu
authors.initials: NT
authors.lastname: Laçin
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000627:therapeutic use; D001419:Bacteria / Q000187:drug effects; D047070:Bandages, Hydrocolloid; D001672:Biocompatible Materials / Q000737:chemistry / Q000627:therapeutic use; D002482:Cellulose / Q000031:analogs & derivatives / Q000737:chemistry / Q000627:therapeutic use*; D002701:Chloramphenicol / Q000737:chemistry* / Q000627:therapeutic use; D006801:Humans; D008567:Membranes, Artificial; D010504:Periodic Acid / Q000737:chemistry; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D008567:Membranes, Artificial; D010504:Periodic Acid; D002701:Chloramphenicol; D002482:Cellulose; C058684:2,3-dialdehydocellulose; C009288:metaperiodate
keywords: 2,3 Dialdehyde bacterial cellulose; Antibacterial activity; Wound healing