title: Maternal schistosomiasis alters costimulatory molecules expression in antigen-presenting cells from adult offspring mice.
abstract: Adult offspring of Schistosoma mansoni-infected mice showed alterations in immunity to a heterologous antigen, ovalbumin (OA). Prior breastfeeding induced increased production of anti-OA antibodies, while pregnancy impaired it. Here, we investigated the expression of costimulatory molecules on antigen-presenting cells (APCs) of the adult offspring of S. mansoni-infected mothers in response to OA. Newborn mice were divided into three groups: animals Born Infected Mothers (BIM) suckled by non-infected mothers; animals from non-infected mothers Suckled Infected Mothers (SIM); and another group of mice born from and suckled by non-infected mothers (CONTROL). The adult offspring were immunized with subcutaneous OA+adjuvant, and 3-8days following immunization, double labeling was performed (CD45R/B220 or CD11c and CD80, CD86, CD40 or HLA-DR) on spleen cells. In comparison to the CONTROL group, an early increased frequency of CD40+/CD80+ B cells was observed in SIM mice (p<0.001/p<0.05), but no alteration of CD11c+ cells was observed. In contrast, in BIM mice, the frequency of CD86+/CD11c+ cells (p<0.05) and CD40+/CD80+/CD86+ B cells (p<0.01/p<0.01/p<0.05) was drastically reduced. In conclusion, previous suckling by S. mansoni-infected mothers enabled improved antigen presentation by B cells in adult offspring, whereas gestation in these mothers imprinted offspring with weak antigen presentation by APCs during the immune response to a non-related antigen.
authors.affiliation: Laboratory of Immunology, Laboratory of Immunopathology Keizo Asami (LIKA), Federal University of Pernambuco, Recife, PE, Brazil.
authors.forename: Patrícia d'Emery Alves
authors.initials: Pd
authors.lastname: Santos
authors.affiliation: Aggeu Magalhães Research Center (CPqAM), Oswaldo Cruz Foundation (FIOCRUZ), Recife, PE, Brazil.
authors.forename: Virgínia Maria Barros
authors.initials: VM
authors.lastname: Lorena
authors.affiliation: Laboratory of Immunology, Laboratory of Immunopathology Keizo Asami (LIKA), Federal University of Pernambuco, Recife, PE, Brazil.
authors.forename: Érica
authors.initials: É
authors.lastname: Fernandes
authors.affiliation: Laboratory of Immunology, Laboratory of Immunopathology Keizo Asami (LIKA), Federal University of Pernambuco, Recife, PE, Brazil.
authors.forename: Iana Rafaela Fernandes
authors.initials: IR
authors.lastname: Sales
authors.affiliation: Laboratory of Immunology, Laboratory of Immunopathology Keizo Asami (LIKA), Federal University of Pernambuco, Recife, PE, Brazil; Department of Tropical Medicine, Health Sciences Center, Federal University of Pernambuco, Recife, PE, Brazil.
authors.forename: Mônica Camelo Pessoa
authors.initials: MC
authors.lastname: Albuquerque
authors.affiliation: Aggeu Magalhães Research Center (CPqAM), Oswaldo Cruz Foundation (FIOCRUZ), Recife, PE, Brazil.
authors.forename: Yara
authors.initials: Y
authors.lastname: Gomes
authors.affiliation: Laboratory of Immunology, Laboratory of Immunopathology Keizo Asami (LIKA), Federal University of Pernambuco, Recife, PE, Brazil; Department of Tropical Medicine, Health Sciences Center, Federal University of Pernambuco, Recife, PE, Brazil.
authors.forename: Vlaudia Maria Assis
authors.initials: VM
authors.lastname: Costa
authors.affiliation: Laboratory of Immunology, Laboratory of Immunopathology Keizo Asami (LIKA), Federal University of Pernambuco, Recife, PE, Brazil; Department of Pharmaceutical Sciences, Health Sciences Center, Federal University of Pernambuco, Recife, PE, Brazil. Electronic address: valdenia.souza@ufpe.br.
authors.forename: Valdênia Maria Oliveira
authors.initials: VM
authors.lastname: Souza
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology*; D015703:Antigens, CD / Q000378:metabolism*; D018122:B7-1 Antigen / Q000378:metabolism; D051940:B7-2 Antigen / Q000378:metabolism; D019013:CD40 Antigens / Q000378:metabolism; D005260:Female; D007112:Immunity, Maternally-Acquired / Q000276:immunology*; D018445:Infectious Disease Transmission, Vertical; D008297:Male; D051379:Mice; D011247:Pregnancy; D015597:Pregnancy Complications, Parasitic / Q000276:immunology*; D012555:Schistosomiasis mansoni / Q000276:immunology* / Q000635:transmission
chemical_list: D015703:Antigens, CD; D018122:B7-1 Antigen; D051940:B7-2 Antigen; D019013:CD40 Antigens
keywords: Adult offspring; Breastfeeding; Costimulatory molecules; Gestation; Maternal schistosomiasis