title: A novel 26RFa peptide containing both analgesic and anti-inflammatory functions from Chinese tree shrew.
abstract: 26RFa is one of neuroendocrine peptide groups in the RFamide peptide family containing conserved Arg-Phe/Tyr-NH2 motif at their C-terminus. They exert multiple biological functions in vertebrates. A novel 26RFa peptide (TC26RFa) with unique structure is identified from the tree shrew of Tupaia belangeri chinensis in the present study. In structure, different from other 26RFa peptides containing conserved Phe-Arg-Phe-NH2 motif at their C-terminus, there is a Phe-Arg-Tyr-NH2 C-terminus in TC26RFa. It has been found that TC26RFa of intraperitoneal injection exerts strong analgesic activities in several mice models including acetic acid-induced abdominal writhing, formalin-induced paw licking, and thermal pain-induced tail withdrawal. It shows comparable analgesic ability with morphine. In addition, this peptide has been found to inhibit inflammatory factor secretion (including tumor necrosis factor-α, interleukin-6, and interleukin-1β) induced by lipopolysaccharides (LPS). Furthermore, it stimulates secretion of the anti-inflammatory factor, interleukin-10. In addition to the identification of a novel 26RFa peptide from tree shrew, a new type of function (anti-inflammation) involved in 26RFa peptide is discovered.
authors.affiliation: Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China; Life Sciences College of Nanjing Agricultural University, Nanjing 210095, Jiangsu, China.
authors.forename: Yuqin
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China; Kunming College of Life Science, University of Chinese Academy of Sciences, Beijing 100009, China.
authors.forename: Zilei
authors.initials: Z
authors.lastname: Duan
authors.affiliation: Life Sciences College of Nanjing Agricultural University, Nanjing 210095, Jiangsu, China.
authors.forename: Guoxiang
authors.initials: G
authors.lastname: Mo
authors.affiliation: Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China; Kunming College of Life Science, University of Chinese Academy of Sciences, Beijing 100009, China.
authors.forename: Chuanbin
authors.initials: C
authors.lastname: Shen
authors.affiliation: Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China.
authors.forename: Longbao
authors.initials: L
authors.lastname: Lv
authors.affiliation: Yunnan Clinical Research Center of Breast Cancer, The Third Affiliated Hospital of Kunming Medical College, Kunming 650032, China.
authors.forename: Wenlin
authors.initials: W
authors.lastname: Chen
authors.affiliation: Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China; Life Sciences College of Nanjing Agricultural University, Nanjing 210095, Jiangsu, China. Electronic address: rlai@mail.kiz.ac.cn.
authors.forename: Ren
authors.initials: R
authors.lastname: Lai
mesh_terms: D000700:Analgesics / Q000008:administration & dosage*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage*; D002681:China; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D008070:Lipopolysaccharides / Q000633:toxicity; D051379:Mice; D009479:Neuropeptides / Q000008:administration & dosage* / Q000737:chemistry; D013623:Tail / Q000187:drug effects / Q000473:pathology; D014418:Tupaiidae
chemical_list: D000700:Analgesics; D000893:Anti-Inflammatory Agents; D008070:Lipopolysaccharides; D009479:Neuropeptides; C480638:prepro-26RFa protein, human
keywords: 26RFa; Analgesic; Inflammation; RFamide peptide; Tree shrew