title: The relationship between compost bedded pack performance, management, and bacterial counts.
abstract: The objective of this study was to assess the relationships among temperature, moisture, carbon-to-nitrogen (C:N) ratio, space per cow, and bacterial counts from bedding material collected from compost bedded pack (CBP) barns. A field survey of 42 routinely aerated CBP barns was conducted in Kentucky between October 2010 and March 2011. Two bedding material samples of 1,064.7 cm(3) each were collected during a single site visit from 9 evenly distributed locations throughout each barn and thoroughly mixed to create a composite sample representative of the entire CBP. Bacterial counts were determined for coliforms, Escherichia coli, streptococci, staphylococci, and Bacillus spp. University of Kentucky Regulatory Services (Lexington) laboratory personnel performed nutrient analyses to determine moisture, carbon, and nitrogen contents. Surface and 10.2-cm pack depth temperatures were collected for each of the 9 evenly distributed locations and the mean calculated to produce a composite temperature. Space per cow was calculated as the total CBP area divided by number of cows housed on the CBP. The GLM procedure of SAS (SAS Institute Inc., Cary, NC) generated models to describe factors affecting bacterial counts. Bacterial counts were 6.3 ± 0.6, 6.0 ± 0.6, 7.2 ± 0.7, 7.9 ± 0.5, and 7.6 ± 0.5 log 10 cfu/g of dry matter for coliform, Escherichia coli, streptococci, staphylococci, and Bacillus spp., respectively. Composite temperature, CBP moisture, C:N ratio, and space per cow had no effect on coliform counts. Escherichia coli reached a peak concentration when the C:N ratio was between 30:1 and 35:1. Staphylococci counts increased as ambient temperature increased. Streptococci counts decreased with increased space per cow and composite temperature and increased with increasing ambient temperature and moisture. Streptococci counts peaked at a C:N ratio ranging from 16:1 to 18:1. Bacillus spp. counts were reduced with increasing moisture, C:N ratio, and ambient temperature. Mastitis-causing bacteria thrive in similar conditions to that of composting bacteria and microbes, making elimination of these at higher temperatures (55 to 65°C) difficult in an active composting environment. Producers must use recommended milking procedures and other preventative practices to maintain low somatic cell count in herds with a CBP barn.
authors.affiliation: Department of Animal and Food Sciences.
authors.forename: R A
authors.initials: RA
authors.lastname: Black
authors.affiliation: Department of Biosystems and Agricultural Engineering, and.
authors.forename: J L
authors.initials: JL
authors.lastname: Taraba
authors.affiliation: Department of Biosystems and Agricultural Engineering, and.
authors.forename: G B
authors.initials: GB
authors.lastname: Day
authors.affiliation: Department of Biosystems and Agricultural Engineering, and.
authors.forename: F A
authors.initials: FA
authors.lastname: Damasceno
authors.affiliation: Department of Animal and Food Sciences.
authors.forename: M C
authors.initials: MC
authors.lastname: Newman
authors.affiliation: Department of Animal and Food Sciences.
authors.forename: K A
authors.initials: KA
authors.lastname: Akers
authors.affiliation: Department of Statistics, University of Kentucky, Lexington 40546.
authors.forename: C L
authors.initials: CL
authors.lastname: Wood
authors.affiliation: Department of Statistics, University of Kentucky, Lexington 40546.
authors.forename: K J
authors.initials: KJ
authors.lastname: McQuerry
authors.affiliation: Department of Animal and Food Sciences. Electronic address: jbewley@uky.edu.
authors.forename: J M
authors.initials: JM
authors.lastname: Bewley
mesh_terms: D000818:Animals; D001407:Bacillus; D058491:Bacterial Load; D002244:Carbon / Q000032:analysis; D002417:Cattle / Q000502:physiology*; D002452:Cell Count; D003612:Dairying / Q000379:methods; D004926:Escherichia coli; D005260:Female; D006799:Housing, Animal; D006813:Humidity; D007629:Kentucky; D008892:Milk / Q000166:cytology / Q000382:microbiology; D009584:Nitrogen / Q000032:analysis; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology; D013210:Staphylococcus; D013291:Streptococcus; D013696:Temperature
chemical_list: D012987:Soil; D002244:Carbon; D009584:Nitrogen
keywords: bacterial analysis; compost bedded pack barn; somatic cell count