title: Characterization of the 17 strains of regulatory T cell-inducing human-derived Clostridia.
abstract: The gut microbiota plays important roles in the development of the host immune system. We have previously shown that a combination of 46 strains of commensal Clostridia isolated from conventionally reared mice can induce the accumulation of CD4(+)Foxp3(+) regulatory T (Treg) cells in the mouse colonic lamina propria. Subsequently, we succeeded in isolating and selecting 17 strains of Clostridia from a healthy human fecal sample that can significantly increase the number and function of colonic Treg cells in colonized rodents, thereby attenuating symptoms of experimental allergic diarrhea and colitis. Here we characterize each of the 17 strains of human-derived Clostridia in terms of sensitivity to antibiotics and ability to produce short chain fatty acids and other metabolites, and discuss their potential as biotherapeutics to correct dysbiosis and treat immune-inflammatory diseases.
authors.affiliation: RIKEN Center for Integrative Medical Sciences (IMS-RCAI); Yokohama, Japan.
authors.forename: Seiko
authors.initials: S
authors.lastname: Narushima
authors.affiliation: Department of Biochemistry; School of Medicine; Keio University; Tokyo, Japan; Japan Science and Technology Agency; Precursory Research for Embryonic Science and Technology (PRESTO); Kawaguchi, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Sugiura
authors.affiliation: Graduate School of Frontier Sciences; The University of Tokyo; Kashiwa, Japan; Japan Science and Technology Agency; Core Research for Evolutional Science and Technology (CREST); Kawaguchi, Japan.
authors.forename: Kenshiro
authors.initials: K
authors.lastname: Oshima
authors.affiliation: RIKEN Center for Integrative Medical Sciences (IMS-RCAI); Yokohama, Japan; Japan Science and Technology Agency; Precursory Research for Embryonic Science and Technology (PRESTO); Kawaguchi, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Atarashi
authors.affiliation: Graduate School of Frontier Sciences; The University of Tokyo; Kashiwa, Japan.
authors.forename: Masahira
authors.initials: M
authors.lastname: Hattori
authors.affiliation: Department of Biochemistry; School of Medicine; Keio University; Tokyo, Japan; Japan Science and Technology Agency; Exploratory Research for Advanced Technology (ERATO); Suematsu Gas Biology Project; Tokyo, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Suematsu
authors.affiliation: RIKEN Center for Integrative Medical Sciences (IMS-RCAI); Yokohama, Japan; Japan Science and Technology Agency; Core Research for Evolutional Science and Technology (CREST); Kawaguchi, Japan.
authors.forename: Kenya
authors.initials: K
authors.lastname: Honda
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D003013:Clostridium / Q000187:drug effects / Q000276:immunology* / Q000302:isolation & purification / Q000502:physiology*; D003106:Colon / Q000382:microbiology; D003600:Cytosol / Q000737:chemistry; D064806:Dysbiosis / Q000628:therapy; D005227:Fatty Acids / Q000032:analysis; D005232:Fatty Acids, Volatile / Q000378:metabolism; D005243:Feces / Q000382:microbiology; D064368:Healthy Volunteers; D006801:Humans; D051379:Mice; D008826:Microbial Sensitivity Tests; D019936:Probiotics / Q000008:administration & dosage; D013559:Symbiosis; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
chemical_list: D000900:Anti-Bacterial Agents; D005227:Fatty Acids; D005232:Fatty Acids, Volatile
keywords: Clostridia; Treg; gut microbiota