title: Comparison of anti-bacterial activity of three types of di-O-caffeoylquinic acids in Lonicera japonica flowers based on microcalorimetry.
abstract: The anti-bacterial activities of three types of di-O-caffeoylquinic acids (diCQAs) in Lonicera japonica flowers, a traditional Chinese medicine (TCM), on Bacillus shigae growth were investigated and compared by microcalorimetry. The three types of diCQAs were 3, 4-di-O-caffeoylquinic acid (3, 4-diCQA), 3, 5-di-O-caffeoylquinic acid (3, 5-diCQA), and 4, 5-di-O-caffeoylquinic acid (4, 5-diCQA). Some qualitative and quantitative information of the effects of the three diCQAs on metabolic power-time curves, growth rate constant k, maximum heat-output power Pm, and the generation time tG, total heat output Qt, and growth inhibitory ratio I of B. shigae were calculated. In accordance with a thermo-kinetic model, the corresponding quantitative relationships of k, Pm, Qt, I and c were established. Also, the half-inhibitory concentrations of the drugs (IC50) were obtained by quantitative analysis. Based on the quantity-activity relationships and the IC50 values, the sequence of inhibitory activity was 3, 5-diCQA > 4, 5-diCQA > 3, 4-diCQA. The results illustrate the possibility that the caffeoyl ester group at C-5 is the principal group that has a higher affinity for the bacterial cell, and that the intramolecular distance of the two caffeoyl ester groups also has an important influence on the anti-bacterial activities of the diCQAs.
authors.affiliation: 302 Military Hospital of China, Beijing 100039, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Han
authors.affiliation: 302 Military Hospital of China, Beijing 100039, China.
authors.forename: Qing-Yuan
authors.initials: QY
authors.lastname: Lv
authors.affiliation: 302 Military Hospital of China, Beijing 100039, China.
authors.forename: Shi-Ying
authors.initials: SY
authors.lastname: Jin
authors.affiliation: 302 Military Hospital of China, Beijing 100039, China.
authors.forename: Tian-Tian
authors.initials: TT
authors.lastname: Zhang
authors.affiliation: 302 Military Hospital of China, Beijing 100039, China.
authors.forename: Shi-Xiao
authors.initials: SX
authors.lastname: Jin
authors.affiliation: Institute for Drug and Instrument Control, Health Department, GLD of PLA, Beijing 100071, China. Electronic address: lxyynz@126.com.
authors.forename: Xian-Yi
authors.initials: XY
authors.lastname: Li
authors.affiliation: 302 Military Hospital of China, Beijing 100039, China. Electronic address: yhlpharm@126.com.
authors.forename: Hai-Long
authors.initials: HL
authors.lastname: Yuan
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001407:Bacillus / Q000187:drug effects* / Q000254:growth & development; D002726:Chlorogenic Acid / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D035264:Flowers / Q000737:chemistry; D020128:Inhibitory Concentration 50; D031239:Lonicera / Q000737:chemistry*; D009005:Monosaccharides / Q000737:chemistry / Q000494:pharmacology*; D011801:Quinic Acid / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology
chemical_list: C500585:3,5-di-O-(E)-caffeoylquinic acid; C500583:4,5-di-O-(E)-caffeoylquinic acid; D000900:Anti-Bacterial Agents; D004365:Drugs, Chinese Herbal; D009005:Monosaccharides; D011801:Quinic Acid; D002726:Chlorogenic Acid; C478100:3,4-di-O-caffeoylquinic acid
keywords: Antibacterial activity; Bacillus shigae; Lonicera japonica; Microcalorimetry; di-O-caffeoylquinic acids