title: CRISPR-Cas system: a powerful tool for genome engineering.
abstract: Targeted gene regulation on a genome-wide scale is a powerful strategy for interrogating, perturbing, and engineering cellular systems. Recent advances with the RNA-mediated Cas9 endonuclease derived from clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated proteins (Cas) systems have dramatically transformed our ability to specifically modify intact genomes of diverse cells and organisms. The CRISPR-Cas system has been adapted as an efficient, facile, and robust gene-targeting technology with the potential for high-throughput and multiplexed genome engineering. Exciting breakthroughs in understanding the mechanisms of the CRISPR-Cas system and its enormous potential for applications across basic science, agricultural and biotechnology.
authors.affiliation: National Centre for Molecular Crop Design, Beijing, 100085, China, ll19841008@163.com.
authors.forename: Liang
authors.initials: L
authors.lastname: Liu
authors.forename: Xiu-Duo
authors.initials: XD
authors.lastname: Fan
mesh_terms: D064113:CRISPR-Cas Systems / Q000235:genetics*; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D005818:Genetic Engineering / Q000379:methods*; D018745:Genome, Plant; D023281:Genomics