title: Isolation, purification, and structural elucidation of polysaccharides from Alhagi-honey.
abstract: The polysaccharide extract (PE) of Uyghur medicinal preparation Alhagi-honey was prepared by water extraction and alcohol precipitation method. The purified polysaccharide AP1-1 was obtained from PE by macroporous adsorption resin chromatography, DEAE cellulose chromatography, and Sephadex gel chromatography; the homogeneity and the molecular weight of AP1-1 were determined by gel filtration; and the acid hydrolysis, periodate oxidation, Smith degradation, and NMR analysis were used to analyze the chemical structure of AP1-1. The result showed that AP1-1 was a homogeneous polysaccharide, whose relative molecular weight was 9.97 × 10(4). Through high-performance capillary electrophoresis analysis, we found that its molecular structure was composed of mannose, glucose, galactose, and galacturonic acid with a molar ratio of about 1.1:1.9:3.9:2.1. The main chain of AP1-1 was mainly made up of → 4)β-d-GalpA-(1 → 4)β-d-GalpA-(1 → 4)-β-d-Galp-(1 → 4)-β-d-Galp-(1 → 6)α-d-Glcp-(1 → 4)α-d-Glcp(1 → , while the side chain is composed of → 6)-α-d-Glcp and 2-CH3-α-d-Man.
authors.affiliation: a Xinjiang Uygur Autonomous Region Product Quality Supervision and Inspection Institute , Urumqi 830002 , China.
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Jian
authors.forename: Jun-Min
authors.initials: JM
authors.lastname: Chang
authors.forename: Mourboul
authors.initials: M
authors.lastname: Ablise
authors.forename: Gai-Ru
authors.initials: GR
authors.lastname: Li
authors.forename: Jia-Wei
authors.initials: JW
authors.lastname: He
mesh_terms: D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005690:Galactose / Q000032:analysis; D005947:Glucose / Q000032:analysis; D006603:Hexuronic Acids / Q000032:analysis; D006722:Honey / Q000032:analysis*; D008358:Mannose / Q000032:analysis; D008970:Molecular Weight; D019906:Nuclear Magnetic Resonance, Biomolecular; D010946:Plants, Medicinal / Q000737:chemistry*; D011134:Polysaccharides / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: C000592306:AP1-1 polysaccharide; D004365:Drugs, Chinese Herbal; D006603:Hexuronic Acids; D011134:Polysaccharides; C007819:galacturonic acid; D005947:Glucose; D008358:Mannose; D005690:Galactose
keywords: Alhagi-honey; polysaccharide; structural elucidation