title: Osteoregenerative capacities of dicalcium phosphate-rich calcium phosphate bone cement.
abstract: Calcium phosphate cement (CPC) is a widely used bone substitute. However, CPC application is limited by poor bioresorption, which is attributed to apatite, the stable product. This study aims to systematically survey the biological performance of dicalcium phosphate (DCP)-rich CPC. DCP-rich CPC exhibited a twofold, surface-modified DCP anhydrous (DCPA)-to-tetracalcium phosphate (TTCP) molar ratio, whereas conventional CPC (c-CPC) showed a onefold, surface unmodified DCPA-to-TTCP molar ratio. Cell adhesion, morphology, viability, and alkaline phosphatase (ALP) activity in the two CPCs were examined with bone cell progenitor D1 cultured in vitro. Microcomputed tomography and histological observation were conducted after CPC implantation in vivo to analyze the residual implant ratio and new bone formation rate. D1 cells cultured on DCP-rich CPC surfaces exhibited higher cell viability, ALP activity, and ALP quantity than c-CPC. Histological evaluation indicated that DCP-rich CPC showed lesser residual implant and higher new bone formation rate than c-CPC. Therefore, DCP-rich CPC can improve bioresorption. The newly developed DCP-rich CPC exhibited potential therapeutic applications for bone reconstruction.
authors.affiliation: College of Dental Medicine, Kaohsiung Medical University, Department of Dentistry, Kaohsiung Medical University Hospital, Kaohsiung, 807, Taiwan; Department of Fiber and Composite Materials, Feng Chia University, Taichung, 407, Taiwan.
authors.forename: Chia-Ling
authors.initials: CL
authors.lastname: Ko
authors.forename: Jian-Chih
authors.initials: JC
authors.lastname: Chen
authors.forename: Yin-Chun
authors.initials: YC
authors.lastname: Tien
authors.forename: Chun-Cheng
authors.initials: CC
authors.lastname: Hung
authors.forename: Jen-Chyan
authors.initials: JC
authors.lastname: Wang
authors.forename: Wen-Cheng
authors.initials: WC
authors.lastname: Chen
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001843:Bone Cements; D001861:Bone Regeneration; D001842:Bone and Bones / Q000201:enzymology; D002130:Calcium Phosphates / Q000737:chemistry*; D051379:Mice; D008807:Mice, Inbred BALB C; D008855:Microscopy, Electron, Scanning
chemical_list: D001843:Bone Cements; D002130:Calcium Phosphates; C020243:calcium phosphate; D000469:Alkaline Phosphatase
keywords: bioresorption; calcium phosphate cements; dicalcium phosphate anhydrous; dicalcium phosphate dihydrate; nanocrystallites