title: Age-related increase in aneuploidy and alteration of gene expression in mouse first polar bodies.
abstract: OBJECTIVE
  To confirm that aneuploidy candidate genes are detectable in the first polar body (PB(1)) of MII oocytes and to investigate the age-dependent molecular changes in PB(1).
  
  
  METHODS
  Aged (12-to 15-mo-old) and young (2-mo-old) mice were administered pregnant mare's serum gonadotropin (PMSG) and human chorionic gonadotrophin (hCG). MII oocytes were obtained and the first PB was removed. mRNA from each PB and its sibling oocyte was reverse transcribed. Real-time PCR was performed to quantify the expression of six genes (BUB1, CDC20, Filia, MCAK, SGOL1, SMC1A) in single PB.
  
  
  RESULTS
  We first demonstrated that detection and quantification of transcripts associated with aneuploidy in single mouse oocyte and sibling PB(1) is possible and the relative abundance of mRNA transcripts in a single PB faithfully reflects the relative abundance of that transcript in its sibling oocyte. We further found that transcript levels were significantly lower in aged PBs compared with young PBs (P<0.05).
  
  
  CONCLUSIONS
  Our results suggest that the detection and analysis of polar body mRNA may provide insight in age-related aneuploidy in oocyte. This analysis is a novel concept to investigate the genesis of chromosome abnormality and could potentially assist in the characterization of mechanisms underlying key molecular origin of female meiotic aneuploidy, which would be of great scientific and clinical value.
authors.forename: Ze-Xu
authors.initials: ZX
authors.lastname: Jiao
authors.forename: Min
authors.initials: M
authors.lastname: Xu
authors.forename: Teresa K
authors.initials: TK
authors.lastname: Woodruff
mesh_terms: D000782:Aneuploidy; D000818:Animals; D005260:Female; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D008540:Meiosis; D051379:Mice; D009865:Oocytes / Q000254:growth & development / Q000378:metabolism; D059705:Polar Bodies / Q000378:metabolism*; D011247:Pregnancy; D014176:Protein Biosynthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis
chemical_list: D012333:RNA, Messenger