title: Expression of adiponectin receptors 1 and 2 in the ovary and concentration of plasma adiponectin during the oestrous cycle of the pig.
abstract: The aim of this study was to compare the expression levels of adiponectin receptor 1 and adiponectin receptor 2 mRNAs and proteins in porcine ovaries during four stages (days 2 to 3, 10 to 12, 14 to 16, 17 to 19) of the oestrous cycle and to measure adiponectin plasma concentrations during the same phases of the cycle. Higher mRNA expression of adiponectin receptor 1 was detected in porcine granulosa cells than in corpora lutea and theca cells (P < 0.01). In contrast, higher gene expression of adiponectin receptor 2 occurred in newly developed and mature corpora lutea (P < 0.01). The adiponectin receptor 1 protein content was the highest in corpora lutea isolated on days 2 to 3 of the cycle and was the lowest in theca interna cells (P < 0.01). The profile of adiponectin receptor 2 protein was similar to that of adiponectin receptor 1. Adiponectin plasma concentrations were significantly higher throughout the luteal phase than in the follicular phase (P < 0.01). In conclusion, the presence of adiponectin receptor 1 and adiponectin receptor 2 mRNAs and proteins in the porcine ovary suggests that adiponectin may directly affect ovarian functions through its own specific receptors. The expression of both receptors and adiponectin plasma concentration were dependent on hormonal status related to the stage of the cycle.
authors.affiliation: University of Warmia and Mazury in Olsztyn Department of Animal Physiology Oczapowskiego 1A 10-719 Olsztyn-Kortowo Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Maleszka
authors.affiliation: University of Warmia and Mazury in Olsztyn Department of Animal Physiology Oczapowskiego 1A 10-719 Olsztyn-Kortowo Poland.
authors.forename: Nina
authors.initials: N
authors.lastname: Smolinska
authors.affiliation: University of Warmia and Mazury in Olsztyn Department of Animal Physiology Oczapowskiego 1A 10-719 Olsztyn-Kortowo Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Nitkiewicz
authors.affiliation: University of Warmia and Mazury in Olsztyn Department of Animal Physiology Oczapowskiego 1A 10-719 Olsztyn-Kortowo Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Kiezun
authors.affiliation: University of Warmia and Mazury in Olsztyn Department of Animal Physiology Oczapowskiego 1A 10-719 Olsztyn-Kortowo Poland.
authors.forename: Kamil
authors.initials: K
authors.lastname: Dobrzyn
authors.affiliation: University of Warmia and Mazury in Olsztyn Department of Animal Physiology Oczapowskiego 1A 10-719 Olsztyn-Kortowo Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Czerwinska
authors.affiliation: University of Warmia and Mazury in Olsztyn Department of Animal Physiology Oczapowskiego 1A 10-719 Olsztyn-Kortowo Poland.
authors.forename: Karol
authors.initials: K
authors.lastname: Szeszko
authors.affiliation: University of Warmia and Mazury in Olsztyn Department of Animal Physiology Oczapowskiego 1A 10-719 Olsztyn-Kortowo Poland.
authors.forename: Tadeusz
authors.initials: T
authors.lastname: Kaminski
keywords: Adiponectin receptors; adiponectin; oestrous cycle; ovary; pig