title: Influence of natural killer cells and perforin‑mediated cytolysis on the development of chemically induced lung cancer in A/J mice.
abstract: One alternative approach for the treatment of lung cancer might be the activation of the immune system using vaccination strategies. However, most of clinical vaccination trials for lung cancer did not reach their primary end points, suggesting that lung cancer is of low immunogenicity. To provide additional experimental information about this important issue, we investigated which type of immune cells contributes to the protection from lung cancer development. Therefore, A/J mice induced for lung adenomas/ adenocarcinomas by the tobacco-specific nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) were depleted of CD4+ or CD8+ T cells, CD11b+ macrophages, Gr-1+ neutrophils and asialo GM1+ natural killer (NK) cells. Subsequent analysis of tumour growth showed an increase in tumour number only in mice depleted of NK cells. Further asking by which mechanism NK cells suppressed tumour development, we neutralized several death ligands of the tumour necrosis factor (TNF) family known to be involved in NK cell-mediated cytotoxicity. However neither depletion of TNF-α, TNF-related apoptosis-inducing ligand, TNF-like weak inducer of apoptosis or FasL alone nor in combination induced an augmentation of tumour burden. To show whether an alternative cell death pathway is involved, we next generated A/J mice deficient for perforin. After challenging with NNK, mice deficient for perforin showed an increase in tumour number and volume compared to wild-type A/J mice. In summary, our data suggest that NK cells and perforin-mediated cytolysis are critically involved in the protection from lung cancer giving promise for further immunotherapeutic strategies for this disease.
authors.forename: Manuela
authors.initials: M
authors.lastname: Frese-Schaper
authors.forename: Andreas
authors.initials: A
authors.lastname: Keil
authors.forename: Hideo
authors.initials: H
authors.lastname: Yagita
authors.forename: Selina Katja
authors.initials: SK
authors.lastname: Steiner
authors.forename: Werner
authors.initials: W
authors.lastname: Falk
authors.forename: Ralph Alexander
authors.initials: RA
authors.lastname: Schmid
authors.forename: Steffen
authors.initials: S
authors.lastname: Frese
mesh_terms: D000230:Adenocarcinoma / Q000139:chemically induced / Q000276:immunology* / Q000473:pathology; D000818:Animals; D017209:Apoptosis; D002273:Carcinogens / Q000633:toxicity; D003602:Cytotoxicity, Immunologic / Q000276:immunology*; D005260:Female; D051858:Forkhead Transcription Factors / Q000378:metabolism; D007694:Killer Cells, Natural / Q000276:immunology*; D008175:Lung Neoplasms / Q000139:chemically induced / Q000276:immunology* / Q000473:pathology; D051379:Mice; D008805:Mice, Inbred A; D009602:Nitrosamines / Q000633:toxicity*; D052899:Pore Forming Cytotoxic Proteins / Q000502:physiology*; D013601:T-Lymphocytes / Q000276:immunology / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D002273:Carcinogens; D051858:Forkhead Transcription Factors; C498833:Foxp3 protein, mouse; D009602:Nitrosamines; D052899:Pore Forming Cytotoxic Proteins; D014409:Tumor Necrosis Factor-alpha; C483886:perforin, mouse; C016583:4-(N-methyl-N-nitrosamino)-1-(3-pyridyl)-1-butanone