title: Crystallization and preliminary X-ray characterization of MutT2, MSMEG_5148 from Mycobacterium smegmatis.
abstract: Crystallization of MutT2, MSMEG_5148 from Mycobacterium smegmatis, has been carried out and the crystals have been characterized using X-ray diffraction. Matthews coefficient calculation suggests the possibility of one protein molecule in the asymmetric unit of the orthorhombic unit cell, space group P2(1)2(1)2 or P2(1)22. Solution of the structure of the protein by molecular replacement using the known three-dimensional structure of a bacterial Nudix hydrolase is envisaged.
authors.affiliation: Molecular Biophysics Unit, Indian Institute of Science, Bangalore 560 012, India.
authors.forename: S M
authors.initials: SM
authors.lastname: Arif
authors.affiliation: Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore 560 012, India.
authors.forename: P B
authors.initials: PB
authors.lastname: Sang
authors.affiliation: Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore 560 012, India.
authors.forename: U
authors.initials: U
authors.lastname: Varshney
authors.affiliation: Molecular Biophysics Unit, Indian Institute of Science, Bangalore 560 012, India.
authors.forename: M
authors.initials: M
authors.lastname: Vijayan
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods*; D020102:Mycobacterium smegmatis / Q000737:chemistry*; D011487:Protein Conformation
chemical_list: D001426:Bacterial Proteins
keywords: MutT2; Mycobacterium smegmatis