title: Evaluation of the antioxidant and anti-osteoporosis activities of chemical constituents of the fruits of Prunus mume.
abstract: The present study investigated the antioxidant and anti-osteoporosis activities of phytochemicals in the fruits of Prunus mume. From the methanol extract, three new acylated sucroses, mumeoses P-R (1-3), were isolated together with 20 known compounds (4-23). Compounds 1-3 showed potent peroxyl radical-scavenging activities and 12-19 showed both potent peroxyl radical-scavenging and reducing activities. The anti-osteoporosis activity was evaluated using murine pre-osteoblastic MC3T3-E1 cells and pre-osteoclastic RAW 264.7 cells. Compounds 2 and 3 (cis-trans isomers), 5, 7, 8, and 10 significantly stimulated the differentiation of pre-osteoblastic MC3T3-E1 cells to increase collagen synthesis or mineralization functions of osteoblasts, while compounds 5, 6, 9, 10, 12, 14-16, 18, 20, and 22 significantly suppressed tartrate-resistant acid phosphatase activity in receptor activator of nuclear factor-κB ligand-induced osteoclastic RAW 264.7 cells. These results indicated that the fruits of P. mume are an excellent source of antioxidant and anti-osteoporosis phytochemicals.
authors.affiliation: College of Pharmacy, Chungnam National University, Daejeon 305-764, Republic of Korea.
authors.forename: Xi-Tao
authors.initials: XT
authors.lastname: Yan
authors.affiliation: Department of Food and Nutrition, Hannam University, Daejeon 305-811, Republic of Korea.
authors.forename: Sang-Hyun
authors.initials: SH
authors.lastname: Lee
authors.affiliation: College of Pharmacy, Chungnam National University, Daejeon 305-764, Republic of Korea.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: College of Pharmacy, Chungnam National University, Daejeon 305-764, Republic of Korea.
authors.forename: Ya-Nan
authors.initials: YN
authors.lastname: Sun
authors.affiliation: College of Pharmacy, Chungnam National University, Daejeon 305-764, Republic of Korea.
authors.forename: Seo-Young
authors.initials: SY
authors.lastname: Yang
authors.affiliation: Department of Food and Nutrition, Hannam University, Daejeon 305-811, Republic of Korea. Electronic address: haedong@hnu.kr.
authors.forename: Hae-Dong
authors.initials: HD
authors.lastname: Jang
authors.affiliation: College of Pharmacy, Chungnam National University, Daejeon 305-764, Republic of Korea. Electronic address: yhk@cnu.ac.kr.
authors.forename: Young-Ho
authors.initials: YH
authors.lastname: Kim
mesh_terms: D016475:3T3 Cells; D000135:Acid Phosphatase / Q000737:chemistry*; D000215:Acylation; D000818:Animals; D000975:Antioxidants / Q000737:chemistry*; D002454:Cell Differentiation / Q000187:drug effects; D003094:Collagen / Q000737:chemistry; D005638:Fruit / Q000737:chemistry*; D007527:Isoenzymes / Q000737:chemistry*; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects; D010024:Osteoporosis / Q000503:physiopathology / Q000517:prevention & control*; D010936:Plant Extracts / Q000494:pharmacology*; D027861:Prunus / Q000737:chemistry*; D000071681:Tartrate-Resistant Acid Phosphatase
chemical_list: D000975:Antioxidants; D007527:Isoenzymes; D010936:Plant Extracts; D003094:Collagen; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase
keywords: Acylated sucrose; Anti-osteoporosis activity; Antioxidant activity; Prunus mume; Rosaceae