title: Metformin plays a dual role in MIN6 pancreatic β cell function through AMPK-dependent autophagy.
abstract: Metformin improves insulin sensitivity in insulin sensitive tissues such as liver, muscle and fat. However, the functional roles and the underlying mechanism of metformin action in pancreatic β cells remain elusive. Here we show that, under normal growth condition, metformin suppresses MIN6 β cell proliferation and promotes apoptosis via an AMPK-dependent and autophagy-mediated mechanism. On the other hand, metformin protects MIN6 cells against palmitic acid (PA)-induced apoptosis. Our findings indicate that metformin plays a dual role in β cell survival and overdose of this anti-diabetic drug itself may lead to potential β cell toxicity.
authors.affiliation: 1. Metabolic Syndrome Research Center and Diabetes Center, Institute of Aging and Geriatric Diseases, Key Laboratory of Diabetes Immunology, Ministry of Education, Institute of Metabolism and Endocrinology, Second Xiangya Hospital, Central South University, Changsha, Hunan, China 410011 ; 2. Endocrinology Department, Zhuzhou Central Hospital, Zhuzhou, Hunan, China 412007.
authors.forename: Yingling
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: 1. Metabolic Syndrome Research Center and Diabetes Center, Institute of Aging and Geriatric Diseases, Key Laboratory of Diabetes Immunology, Ministry of Education, Institute of Metabolism and Endocrinology, Second Xiangya Hospital, Central South University, Changsha, Hunan, China 410011.
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.affiliation: 1. Metabolic Syndrome Research Center and Diabetes Center, Institute of Aging and Geriatric Diseases, Key Laboratory of Diabetes Immunology, Ministry of Education, Institute of Metabolism and Endocrinology, Second Xiangya Hospital, Central South University, Changsha, Hunan, China 410011.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: 1. Metabolic Syndrome Research Center and Diabetes Center, Institute of Aging and Geriatric Diseases, Key Laboratory of Diabetes Immunology, Ministry of Education, Institute of Metabolism and Endocrinology, Second Xiangya Hospital, Central South University, Changsha, Hunan, China 410011.
authors.forename: Zhipeng
authors.initials: Z
authors.lastname: Xu
authors.affiliation: 1. Metabolic Syndrome Research Center and Diabetes Center, Institute of Aging and Geriatric Diseases, Key Laboratory of Diabetes Immunology, Ministry of Education, Institute of Metabolism and Endocrinology, Second Xiangya Hospital, Central South University, Changsha, Hunan, China 410011.
authors.forename: Jieyu
authors.initials: J
authors.lastname: He
authors.affiliation: 2. Endocrinology Department, Zhuzhou Central Hospital, Zhuzhou, Hunan, China 412007.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Lin
authors.affiliation: 1. Metabolic Syndrome Research Center and Diabetes Center, Institute of Aging and Geriatric Diseases, Key Laboratory of Diabetes Immunology, Ministry of Education, Institute of Metabolism and Endocrinology, Second Xiangya Hospital, Central South University, Changsha, Hunan, China 410011.
authors.forename: Zhiguang
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: 1. Metabolic Syndrome Research Center and Diabetes Center, Institute of Aging and Geriatric Diseases, Key Laboratory of Diabetes Immunology, Ministry of Education, Institute of Metabolism and Endocrinology, Second Xiangya Hospital, Central South University, Changsha, Hunan, China 410011.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Zhang
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000378:metabolism*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001343:Autophagy / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D050417:Insulin-Secreting Cells / Q000187:drug effects*; D008297:Male; D008687:Metformin / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D019308:Palmitic Acid; D015398:Signal Transduction
chemical_list: D007004:Hypoglycemic Agents; D019308:Palmitic Acid; D008687:Metformin; D055372:AMP-Activated Protein Kinases
keywords: AMPK; apoptosis; autophagy; metformin; pancreatic β cells