title: Laccase immobilized on a PAN/adsorbents composite nanofibrous membrane for catechol treatment by a biocatalysis/adsorption process.
abstract: The treatment of catechol via biocatalysis and adsorption with a commercial laccase immobilized on polyacrylonitrile/montmorillonite/graphene oxide (PAN/MMT/GO) composite nanofibers was evaluated with a homemade nanofibrous membrane reactor. The properties in this process of the immobilized laccase on PAN, PAN/MMT as well as PAN/MMT/GO with different weight ratios of MMT and GO were investigated. These membranes were successfully applied for removal of catechol from an aqueous solution. Scanning electron microscope images revealed different morphologies of the enzyme aggregates on different supports. After incorporation of MMT or MMT/GO, the optimum pH showed an alkaline shift to 4, compared to 3.5 for laccase immobilized on pure PAN nanofibers. The optimum temperature was at 55 °C for all the immobilized enzymes. Besides, the addition of GO improved the operational stability and storage stability. A 39% ± 2.23% chemical oxygen demand (COD) removal from the catechol aqueous solution was achieved. Experimental results suggested that laccase, PAN, adsorbent nanoparticles (MMT/GO) can be combined together for catechol treatment in industrial applications.
authors.affiliation: Key Laboratory of Eco-Textiles, Ministry of Education, Jiangnan University, Wuxi 214122, China. wqq888217@126.com.
authors.forename: Qingqing
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Key Laboratory of Eco-Textiles, Ministry of Education, Jiangnan University, Wuxi 214122, China. wykaojn@126.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Cui
authors.affiliation: Key Laboratory of Eco-Textiles, Ministry of Education, Jiangnan University, Wuxi 214122, China.
authors.forename: Guohui
authors.initials: G
authors.lastname: Li
authors.affiliation: Key Laboratory of Eco-Textiles, Ministry of Education, Jiangnan University, Wuxi 214122, China. qq164542493@126.com.
authors.forename: Jinning
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Eco-Textiles, Ministry of Education, Jiangnan University, Wuxi 214122, China. ldw19900323@163.com.
authors.forename: Dawei
authors.initials: D
authors.lastname: Li
authors.affiliation: Key Laboratory of Eco-Textiles, Ministry of Education, Jiangnan University, Wuxi 214122, China. windhuang325@163.com.
authors.forename: Fenglin
authors.initials: F
authors.lastname: Huang
authors.affiliation: Key Laboratory of Eco-Textiles, Ministry of Education, Jiangnan University, Wuxi 214122, China. qfwei@jiangnan.edu.cn.
authors.forename: Qufu
authors.initials: Q
authors.lastname: Wei
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D000327:Adsorption; D001546:Bentonite / Q000737:chemistry; D055162:Biocatalysis; D002396:Catechols / Q000737:chemistry*; D004789:Enzyme Activation; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism*; D006108:Graphite / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D042845:Laccase / Q000737:chemistry / Q000378:metabolism*; D008567:Membranes, Artificial; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D013696:Temperature
chemical_list: D000180:Acrylic Resins; D002396:Catechols; D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; D001546:Bentonite; C010504:polyacrylonitrile; D006108:Graphite; D042845:Laccase; C034221:catechol