title: In vitro assessment of the biologic activity of interferon beta formulations used for the treatment of relapsing multiple sclerosis.
abstract: A new formulation (NF) of subcutaneous (sc) interferon (IFN) β-1a was developed in an attempt to improve injection tolerability and immunogenicity. We compared antiviral and IFNβ-stimulated gene (ISG) activities of IFNβ-1a sc NF with IFNβ-1a sc original formulation and IFNβ-1b sc. When equivalent unit amounts were compared, the IFNβ formulations demonstrated similar antiviral activity and induced similar levels of ISG mRNA. However, on a weight basis (ng/mL), significantly more IFNβ-1b sc was needed to equal the antiviral activity of either IFNβ-1a sc formulation, and both IFNβ-1a sc formulations induced significantly higher levels of ISG mRNA than IFNβ-1b sc.
authors.affiliation: a Department of Molecular Medicine , Sapienza University of Rome , Rome , Italy.
authors.forename: Carolina
authors.initials: C
authors.lastname: Scagnolari
authors.forename: Carla
authors.initials: C
authors.lastname: Selvaggi
authors.forename: Emilia
authors.initials: E
authors.lastname: Di Biase
authors.forename: Maurizio
authors.initials: M
authors.lastname: Fraulo
authors.forename: Fernando
authors.initials: F
authors.lastname: Dangond
authors.forename: Guido
authors.initials: G
authors.lastname: Antonelli
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D004847:Epithelial Cells / Q000187:drug effects* / Q000276:immunology / Q000821:virology; D005347:Fibroblasts / Q000187:drug effects* / Q000276:immunology / Q000821:virology; D015870:Gene Expression; D006801:Humans; D000068556:Interferon beta-1a; D000068576:Interferon beta-1b; D050832:Interferon-Stimulated Gene Factor 3 / Q000819:agonists / Q000096:biosynthesis / Q000276:immunology; D016899:Interferon-beta / Q000276:immunology / Q000494:pharmacology*; D051379:Mice; D009103:Multiple Sclerosis / Q000188:drug therapy; D012008:Recurrence; D014721:Vesicular stomatitis Indiana virus / Q000276:immunology; D019562:Viral Load / Q000187:drug effects*; D014779:Virus Replication / Q000187:drug effects
chemical_list: D050832:Interferon-Stimulated Gene Factor 3; D000068576:Interferon beta-1b; D016899:Interferon-beta; D000068556:Interferon beta-1a