title: Antitumor immune activity by chemokine CX3CL1 in an orthotopic implantation of lung cancer modelin vivo.
abstract: Due to their chemoattractant properties stimulating the accumulation of infiltrating immune cells in tumors, chemokines are known to have antitumor effects. Fractalkine, a unique CX3C chemokine, is expressed in activated endothelial cells, while its receptor, CX3CR1, is expressed in cytolytic immune cells, such as natural killer cells, monocytes and some CD8+T cells. The biological properties of cancer cells are affected by the implantation organ and differences in immune systems, requiring cancer implantation in orthotopic organs in anin vivoexperiment. To develop new therapy strategies for lung cancer, an animal model reflecting the clinical features of lung cancer was previously established. This study aimed to determine whether CX3CL1-induced biological functions should be used for immune cell-based gene therapy of lung cancer in the orthotopic implantation model. An orthotopic intrapulmonary implantation of CX3CL1-stable expression in mouse lung cancer (LLC-CX3CL1) was performed to analyze growth. Results showed a significant decrease in tumor growth in the lung compared to the control cells (LLC-mock). Furthermore, the antitumor effects of CX3CL1 were derived from natural killer cell activities in the depletion experimentin vivo. Therefore, CX3CL1 has the potential of a useful therapeutic target in lung cancer.
authors.affiliation: Divisions of Pathogenic Biochemistry and.
authors.forename: Ji-Ye
authors.initials: JY
authors.lastname: Kee
authors.affiliation: Divisions of Pathogenic Biochemistry and.
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Arita
authors.affiliation: Divisions of Pathogenic Biochemistry and.
authors.forename: Kanna
authors.initials: K
authors.lastname: Shinohara
authors.affiliation: Divisions of Pathogenic Biochemistry and.
authors.forename: Yasukata
authors.initials: Y
authors.lastname: Ohashi
authors.affiliation: Divisions of Pathogenic Biochemistry and ; Department of Cancer Cell Biology, Graduate School Medicine and Pharmaceutical Sciences, University of Toyama, Toyama 930-0194, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Sakurai
authors.affiliation: Divisions of Pathogenic Biochemistry and.
authors.forename: Ikuo
authors.initials: I
authors.lastname: Saiki
authors.affiliation: Divisions of Pathogenic Biochemistry and ; Kampo Diagnostics, Institute of Natural Medicine.
authors.forename: Keiichi
authors.initials: K
authors.lastname: Koizumi
keywords: CX3CL1; chemokine; gene therapy; orthotopic implantation