title: Extraction of total DNA and optimization of the RAPD reaction system in Dioscorea opposita Thunb.
abstract: Dioscorea opposita Thunb. has been used as health food and herbal medicinal ingredients in traditional Chinese medicine. In this study, the total DNA of D. opposita Thunb. was extracted using an improved cetyltrimethylammonium bromide (CTAB) method, and the extracted DNA was further used for random amplified polymorphic DNA (RAPD) reaction system by design of the L16 (4(4)) orthogonal diagram. The results showed that the improved CTAB method can be used to isolate high-quality and high-concentration DNA, and the optimized protocol can overcome the instability of RAPD reaction system. The knowledge stated here can be used to study the genetic diversity of D. opposita Thunb.
authors.affiliation: College of Life Science, China West Normal University, Nanchong, China.
authors.forename: G Q
authors.initials: GQ
authors.lastname: Wen
authors.affiliation: College of Life Science, China West Normal University, Nanchong, China.
authors.forename: J
authors.initials: J
authors.lastname: Li
authors.affiliation: College of Life Science, China West Normal University, Nanchong, China liuxiaohong0817@aliyun.com.
authors.forename: X H
authors.initials: XH
authors.lastname: Liu
authors.affiliation: College of Life Science, China West Normal University, Nanchong, China.
authors.forename: Y S
authors.initials: YS
authors.lastname: Zhang
authors.affiliation: Rice and Sorghum Research Institute, Sichuan Academy of Agricultural Sciences, Deyang, China.
authors.forename: S S
authors.initials: SS
authors.lastname: Wen
mesh_terms: D018548:Cotyledon / Q000235:genetics; D018744:DNA, Plant / Q000302:isolation & purification*; D027742:Dioscorea / Q000145:classification / Q000235:genetics*; D006801:Humans; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*
chemical_list: D018744:DNA, Plant