title: Analytical method and interlaboratory study for the quantitation of aminocyclopyrachlor residues in vegetation by liquid chromatography/tandem mass spectrometry.
abstract: The quick, easy, cheap, effective, rugged, and safe (QuEChERS) and the U.S. Food and Drug Administration pesticide multiresidue methods were systematically tested for the analysis of aminocyclopyrachlor, a new synthetic auxin herbicide. Results revealed that these procedures are not suitable, highlighting the need for a publicly available residue method for this new active ingredient. Such a method has been developed and is reported herein for aminocyclopyrachlor residue analysis in vegetation, including white pine, Norway spruce, grass forage, and grass hay. Aminocyclopyrachlor residues were purified from plant tissue extracts by filtration through SPE cartridges with strong anion exchange stationary phases and analyzed using LC/MS/MS. This method was validated at five laboratories across the United States; their results were compared to evaluate reproducibility and ruggedness. The method LOQ was 0.01 mg/kg (ppm) and the LOD 0.003 mg/kg (ppm) for the matrixes tested. A qualitative procedure to confirm the molecular identity of residues based on retention time measurements and ion transition peak area ratios (m/z 214 --> 681m/z 214 --> 101) is also presented.
authors.forename: Sergio C
authors.initials: SC
authors.lastname: Nanita
authors.forename: Dustin J
authors.initials: DJ
authors.lastname: Stewart
authors.forename: Carol A
authors.initials: CA
authors.lastname: Rodgers
authors.forename: Elen
authors.initials: E
authors.lastname: Tesfai
authors.forename: Timo
authors.initials: T
authors.lastname: Schwarz
authors.forename: Erin C
authors.initials: EC
authors.lastname: Robaugh
authors.forename: Robert M
authors.initials: RM
authors.lastname: Henze
authors.forename: Anne M
authors.initials: AM
authors.lastname: Pentz
authors.forename: Thomas
authors.initials: T
authors.lastname: Moate
authors.forename: Emily
authors.initials: E
authors.lastname: Vogl
authors.forename: Randy
authors.initials: R
authors.lastname: Weintraub
authors.forename: David
authors.initials: D
authors.lastname: Robaugh
authors.forename: Amanda
authors.initials: A
authors.lastname: Krening
authors.forename: Edison
authors.initials: E
authors.lastname: Hidalgo
authors.forename: Thomas
authors.initials: T
authors.lastname: Class
authors.forename: Paul
authors.initials: P
authors.lastname: Reibach
authors.forename: Tracy
authors.initials: T
authors.lastname: McLachlan
authors.forename: Christopher
authors.initials: C
authors.lastname: Lowrie
mesh_terms: D002138:Calibration; D002852:Chromatography, Ion Exchange / Q000379:methods; D002853:Chromatography, Liquid / Q000379:methods*; D004784:Environmental Monitoring / Q000379:methods; D005374:Filtration; D006540:Herbicides / Q000737:chemistry*; D007477:Ions; D007753:Laboratories / Q000592:standards; D010573:Pesticide Residues / Q000032:analysis*; D006109:Poaceae / Q000737:chemistry; D011743:Pyrimidines / Q000032:analysis*; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014197:Trees / Q000737:chemistry; D014481:United States
chemical_list: D006540:Herbicides; D007477:Ions; D010573:Pesticide Residues; D011743:Pyrimidines; C560341:aminocyclopyrachlor