title: Molecular and morphologic identification of Pentatrichomonas hominis in swine.
abstract: A marasmic pig with watery diarrhea was identified to harbor the human-pathogenic protist Pentatrichomonas hominis by PCR and sequence analysis of three genetic loci (ITS1-5.8S rRNA-ITS2, 18S rRNA, and EF-1α), electron microscopy, and infection experiments. DNA sequencing and phylogenetic analysis indicated the organism isolated in this study was most closely related to P. hominis. SEM and TEM observation of the ultrastructure demonstrated that it had a morphology identical to P. hominis. The result of experimental infections with P. hominis exhibited that the cells had the ability to propagate in the cecum of piglets and fecal-oral route might be the major way in which pigs became infected. The present study confirmed that swine could be a host for P. hominis and might serve as a reservoir for human trichomoniasis.
authors.affiliation: College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun 130062, China.
authors.forename: Weizhi
authors.initials: W
authors.lastname: Li
authors.affiliation: College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun 130062, China; College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, Harbin 150030, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun 130062, China.
authors.forename: Pengtao
authors.initials: P
authors.lastname: Gong
authors.affiliation: College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun 130062, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Meng
authors.affiliation: College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun 130062, China.
authors.forename: Wenchao
authors.initials: W
authors.lastname: Li
authors.affiliation: College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun 130062, China.
authors.forename: Chengcai
authors.initials: C
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun 130062, China; College of Life Science, Northeast Agricultural University, 59 Mucai Street, Harbin 150030, China.
authors.forename: Shijie
authors.initials: S
authors.lastname: Li
authors.affiliation: College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun 130062, China.
authors.forename: Ju
authors.initials: J
authors.lastname: Yang
authors.affiliation: College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun 130062, China.
authors.forename: He
authors.initials: H
authors.lastname: Li
authors.affiliation: College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun 130062, China. Electronic address: xczhang@jlu.edu.cn.
authors.forename: Xichen
authors.initials: X
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun 130062, China. Electronic address: jianhuali7207@163.com.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Li
mesh_terms: D000818:Animals; D002432:Cecum / Q000469:parasitology; D020459:Genes, rRNA / Q000235:genetics; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D010802:Phylogeny; D011529:Protozoan Infections, Animal / Q000469:parasitology*; D011897:Random Allocation; D013552:Swine; D013553:Swine Diseases / Q000469:parasitology*; D016834:Trichomonadida / Q000145:classification / Q000235:genetics* / Q000648:ultrastructure*
keywords: Electron microscopy; Experimental infection; PCR; Pentatrichomonas hominis; Phylogeny