title: Testing a new formulation for Peste des Petits Ruminants vaccine in Ethiopia.
abstract: In this paper extended tests on a new candidate formulation for Peste des Petits Ruminants (PPR) vaccine carried out at National Veterinary Institute (NVI) in Ethiopia are presented. This work was performed in the frame of the VACNADA project from GALVmed which aimed at procuring vaccines against neglected veterinary diseases to African vaccine producing laboratories, in particular PPR. After the eradication of Rinderpest, Peste des Petits Ruminants became the next veterinary disease on target for elimination, requiring an effective and thermostable vaccine. In this work a Tris/Trehalose formulation was evaluated in thermal stability studies in comparison to the current used formulation of the live attenuated PPR vaccine, the Weybridge medium. The extended results presented herein show an increased thermal stability of the vaccine, especially at 37 and 45 °C, as expected from previously published results (Silva A.C. et al., 2011). Furthermore, during the course of this project, the NVI teams have clearly demonstrated ability to produce higher quality PPR vaccines after a successful transfer of the technology. These results should significantly enhance the utility of the vaccine in the eradication of PPR.
authors.affiliation: Instituto de Biologia Experimental e Tecnológica (IBET), Animal Cell Technology Unit, Apartado 12, P-2781-901 Oeiras, Portugal; Instituto de Tecnologia Química e Biológica-Universidade de Lisboa (ITQB-UNL), Animal Cell Technology Unit, Apartado 12, P-2781-901 Oeiras, Portugal.
authors.forename: Ana Carina
authors.initials: AC
authors.lastname: Silva
authors.affiliation: National Veterinary Institute (NVI), Debre Zeit, P.O. Box 19, Ethiopia.
authors.forename: Martha
authors.initials: M
authors.lastname: Yami
authors.affiliation: CIRAD Department EMVT, Control of Exotic and Emerging Animal Diseases (UPR15), TA 30/G, Campus International de Baillarguet, 34398 Montpellier Cedex 05, France.
authors.forename: Geneviève
authors.initials: G
authors.lastname: Libeau
authors.affiliation: Instituto de Biologia Experimental e Tecnológica (IBET), Animal Cell Technology Unit, Apartado 12, P-2781-901 Oeiras, Portugal; FCT/UNL, Laboratório de Engenharia Bioquímica, P-2825 Monte da Caparica, Portugal.
authors.forename: Manuel J T
authors.initials: MJ
authors.lastname: Carrondo
authors.affiliation: Instituto de Biologia Experimental e Tecnológica (IBET), Animal Cell Technology Unit, Apartado 12, P-2781-901 Oeiras, Portugal; Instituto de Tecnologia Química e Biológica-Universidade de Lisboa (ITQB-UNL), Animal Cell Technology Unit, Apartado 12, P-2781-901 Oeiras, Portugal. Electronic address: marques@itqb.unl.pt.
authors.forename: Paula M
authors.initials: PM
authors.lastname: Alves
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D005002:Ethiopia; D029021:Peste-des-Petits-Ruminants / Q000517:prevention & control*; D013696:Temperature; D064166:Vaccine Potency; D014613:Vaccines, Attenuated / Q000737:chemistry; D014709:Vero Cells; D014765:Viral Vaccines / Q000737:chemistry*
chemical_list: D014613:Vaccines, Attenuated; D014765:Viral Vaccines
keywords: Cold chain; Formulation; Peste des Petits Ruminants vaccine; Stability; Technology transfer