title: Imaging lipids with secondary ion mass spectrometry.
abstract: This review discusses the application of time-of-flight secondary ion mass spectrometry (TOF-SIMS) and magnetic sector SIMS with high lateral resolution performed on a Cameca NanoSIMS 50(L) to imaging lipids. The similarities between the two SIMS approaches and the differences that impart them with complementary strengths are described, and various strategies for sample preparation and to optimize the quality of the SIMS data are presented. Recent reports that demonstrate the new insight into lipid biochemistry that can be acquired with SIMS are also highlighted. This article is part of a Special Issue entitled Tools to study lipid functions.
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA; Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA. Electronic address: mlkraft@illinois.edu.
authors.forename: Mary L
authors.initials: ML
authors.lastname: Kraft
authors.affiliation: Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
authors.forename: Haley A
authors.initials: HA
authors.lastname: Klitzing
mesh_terms: D008055:Lipids / Q000737:chemistry*; D008280:Magnetics; D036103:Nanotechnology; D018629:Spectrometry, Mass, Secondary Ion / Q000379:methods*
chemical_list: D008055:Lipids
keywords: Cell membrane; Lipid distribution; Model membrane; NanoSIMS; Sample preparation; TOF-SIMS