title: Synthesis, cytotoxicity, and hydroxyapatite formation in 27-Tris-SBF for sol-gel based CaO-P2O5-SiO2-B2O3-ZnO bioactive glasses.
abstract: CaO-P2O5-SiO2-B2O3-ZnO bioactive glasses were prepared via an optimized sol-gel method. The current investigation was focused on producing novel zinc based calcium phosphoborosilicate glasses and to evaluate their mechanical, rheological, and biocompatible properties. The morphology and composition of these glasses were studied using X-ray diffraction (XRD) and scanning electron microscopy (SEM). The particle size, mechanical and flexural strength was also determined. Furthermore, the zeta potential of all the glasses were determined to estimate their flocculation tendency. The thermal analysis and weight loss measurements were carried out using differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA) respectively. For assessing the in-vitro bioactive character of synthesized glasses, the ability for apatite formation on their surface upon their immersion in simulated body fluid (SBF) was checked using SEM and pH measurements. MTS assay cytotoxicity assay and live-dead cell viability test were conducted on J774A.1 cells murine macrophage cells for different glass concentrations.
authors.affiliation: Department of Material Science and Engineering, Holden Hall, Virginia Polytechnic Institute and State University, Blacksburg, Virginia-24060, USA.
authors.forename: Gurbinder
authors.initials: G
authors.lastname: Kaur
authors.affiliation: Department of Material Science and Engineering, Holden Hall, Virginia Polytechnic Institute and State University, Blacksburg, Virginia-24060, USA.
authors.forename: G
authors.initials: G
authors.lastname: Pickrell
authors.affiliation: Department of Biomedical Sciences and Pathobiology, Virginia Polytechnic Institute and State University, Blacksburg, Virginia-24060, USA.
authors.forename: G
authors.initials: G
authors.lastname: Kimsawatde
authors.affiliation: Department of Material Science and Engineering, Holden Hall, Virginia Polytechnic Institute and State University, Blacksburg, Virginia-24060, USA.
authors.forename: D
authors.initials: D
authors.lastname: Homa
authors.affiliation: Department of Material Science and Engineering, Holden Hall, Virginia Polytechnic Institute and State University, Blacksburg, Virginia-24060, USA.
authors.forename: H A
authors.initials: HA
authors.lastname: Allbee
authors.affiliation: Department of Biomedical Sciences and Pathobiology, Virginia Polytechnic Institute and State University, Blacksburg, Virginia-24060, USA.
authors.forename: N
authors.initials: N
authors.lastname: Sriranganathan
mesh_terms: D000818:Animals; D040761:Biomimetic Materials / Q000737:chemistry; D001826:Body Fluids / Q000737:chemistry; D001896:Boron Compounds / Q000737:chemistry / Q000494:pharmacology; D017610:Calcium Compounds / Q000737:chemistry / Q000494:pharmacology; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D017886:Durapatite / Q000138:chemical synthesis*; D005898:Glass / Q000737:chemistry*; D008264:Macrophages / Q000166:cytology / Q000187:drug effects; D008422:Materials Testing; D051379:Mice; D010087:Oxides / Q000737:chemistry / Q000494:pharmacology; D044367:Phase Transition; D017553:Phosphorus Compounds / Q000737:chemistry / Q000494:pharmacology; D017640:Silicates / Q000737:chemistry* / Q000494:pharmacology; D012822:Silicon Dioxide / Q000737:chemistry / Q000494:pharmacology; D054457:Tissue Scaffolds; D015034:Zinc Oxide / Q000737:chemistry / Q000494:pharmacology
chemical_list: D001896:Boron Compounds; D017610:Calcium Compounds; D010087:Oxides; D017553:Phosphorus Compounds; D017640:Silicates; D012822:Silicon Dioxide; D017886:Durapatite; C016538:lime; D015034:Zinc Oxide