title: Expression of voltage-dependent potassium channels in first trimester human placentae.
abstract: Potassium channel α-subunits encoded by KCNQ1-5 genes form voltage-dependent channels (KV7), modulated by KCNE1-5 encoded accessory proteins. The aim was to determine KCNQ and KCNE mRNA expression and assess protein expression/localisation of the KCNQ3 and KCNE5 isoforms in first trimester placental tissue. Placentae were obtained from women undergoing elective surgical termination of pregnancy (TOP) at ≤ 10 weeks' (early TOP) and >10 weeks' (mid TOP) gestations. KCNQ1-5 expression was unchanged during the first trimester. KCNE5 expression increased in mid TOP vs. early TOP samples (P = 0.022). This novel study reports mRNA and protein expression of KV7 channels in first trimester placentae.
authors.affiliation: Division of Women's Health, King's College London, Women's Health Academic Centre, KHP, St Thomas' Hospital, Westminster Bridge Road, London SE1 7EH, UK; Department of Nephrology, Hypertension, Clinical Pharmacology and of Clinical Research, University of Bern, Berne CH-3010, Switzerland. Electronic address: hiten.mistry@kcl.ac.uk.
authors.forename: H D
authors.initials: HD
authors.lastname: Mistry
authors.affiliation: Department of Obstetrics & Gynaecology, School of Medicine, University of Nottingham, Nottingham NG5 1PB, UK.
authors.forename: L O
authors.initials: LO
authors.lastname: Kurlak
authors.affiliation: Division of Biomedical Sciences, St George's University of London, London SW17 0RE, UK.
authors.forename: G S
authors.initials: GS
authors.lastname: Whitley
authors.affiliation: Division of Biomedical Sciences, St George's University of London, London SW17 0RE, UK.
authors.forename: J E
authors.initials: JE
authors.lastname: Cartwright
authors.affiliation: Department of Obstetrics & Gynaecology, School of Medicine, University of Nottingham, Nottingham NG5 1PB, UK.
authors.forename: F
authors.initials: F
authors.lastname: Broughton Pipkin
authors.affiliation: Division of Women's Health, King's College London, Women's Health Academic Centre, KHP, St Thomas' Hospital, Westminster Bridge Road, London SE1 7EH, UK.
authors.forename: R M
authors.initials: RM
authors.lastname: Tribe
mesh_terms: D000328:Adult; D005260:Female; D006801:Humans; D010920:Placenta / Q000378:metabolism*; D024642:Potassium Channels, Voltage-Gated / Q000235:genetics / Q000378:metabolism*; D011247:Pregnancy; D011261:Pregnancy Trimester, First / Q000235:genetics / Q000378:metabolism*
chemical_list: D024642:Potassium Channels, Voltage-Gated
keywords: Early pregnancy; KCNE; KCNQ; Placenta; Potassium channel