title: The effect of Galleria mellonella hemolymph polypeptides on Legionella gormanii.
abstract: Among Legionella species, which are recognized to be pathogenic for humans, L. gormanii is the second prevalent causative agent of community-acquired pneumonia after L. pneumophila. Anti-L. gormanii activity of Galleria mellonella hemolymph extract and apolipophorin III (apoLp-III) was examined. The extract and apoLp-III at the concentration 0.025 mg/ml caused 75% and 10% decrease of the bacteria survival rate, respectively. The apoLp-III-induced changes of the bacteria cell surface were analyzed for the first time by atomic force microscopy. Our studies demonstrated the powerful anti-Legionella effects of the insect defence polypeptides, which could be exploited in drugs design against these pathogens.
authors.affiliation: Department of Genetics and Microbiology, Institute of Microbiology and Biotechnology, Maria Curie-Skłodowska University, Lublin, Poland.
authors.forename: Elżbieta
authors.initials: E
authors.lastname: Chmiel
authors.affiliation: Department of Genetics and Microbiology, Institute of Microbiology and Biotechnology, Maria Curie-Skłodowska University, Lublin, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Palusinska-Szysz
authors.affiliation: Department of Immunobiology, Institute of Biology and Biochemistry, Maria Curie-Skłodowska University, Lublin, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Zdybicka-Barabas
authors.affiliation: Department of Immunobiology, Institute of Biology and Biochemistry, Maria Curie-Skłodowska University, Lublin, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Cytryńska
authors.affiliation: Department of Analytical Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland.
authors.forename: Paweł
authors.initials: P
authors.lastname: Mak
mesh_terms: D000818:Animals; D001053:Apolipoproteins / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects* / Q000648:ultrastructure; D006458:Hemolymph / Q000737:chemistry; D007875:Legionella / Q000187:drug effects* / Q000472:pathogenicity / Q000648:ultrastructure; D007915:Lepidoptera / Q000737:chemistry; D018625:Microscopy, Atomic Force; D010455:Peptides / Q000008:administration & dosage* / Q000737:chemistry
chemical_list: D001053:Apolipoproteins; D010455:Peptides; C042470:apolipophorin III