title: [Establishment of a quantitative detection method for Golgi protein73].
abstract: OBJECTIVE
  To establish enzyme-linked immunosorbent assay (ELISA) for quantitative detection of Golgi protein73 (GP73) in serum.
  
  
  METHODS
  A sandwich reaction was preformed with horseradish peroxidase labeled monoclonal antibody of GP73 as the catalytic enzyme. Several reactions liquid's concentration and reaction conditions were optimized. The method was evaluated in all aspects such as linear range, sensitivity, specificity, stability and so on.
  
  
  RESULTS
  The linear range was 25-500 ng/ml. The detection limit was 18.5 ng/ml. Inter-assay and intra-assay RSD were both less than 10%. The recoveries of three different spiked concentration samples were 95.3%, 92.6% and 103.7%. After stored at 4 degrees C and 37 degrees C for 3, 5, 7 days, the analysis showed correlation coefficient higher than 0.98 and RSD lower than 10%.
  
  
  CONCLUSIONS
  Established ELISA for quantity determination of serum GP73 has high accuracy, sensitivity and repeatability.
authors.forename: Jun
authors.initials: J
authors.lastname: Hou
authors.forename: Tong-Sheng
authors.initials: TS
authors.lastname: Guo
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.forename: Ai-Xia
authors.initials: AX
authors.lastname: Liu
authors.forename: Yong-Ji
authors.initials: YJ
authors.lastname: Song
authors.forename: Jing-Xia
authors.initials: JX
authors.lastname: Guo
authors.forename: Jia
authors.initials: J
authors.lastname: Liu
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
authors.forename: Jun
authors.initials: J
authors.lastname: Xu
authors.forename: Hong-Shan
authors.initials: HS
authors.lastname: Wei
authors.forename: Bo-An
authors.initials: BA
authors.lastname: Li
mesh_terms: D000911:Antibodies, Monoclonal / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D008565:Membrane Proteins / Q000097:blood*; D012680:Sensitivity and Specificity
chemical_list: D000911:Antibodies, Monoclonal; C410824:GOLM1 protein, human; D008565:Membrane Proteins