title: Lanthanide-based nanocrystals as dual-modal probes for SPECT and X-ray CT imaging.
abstract: Applications of lanthanide-based nanoparticles for bioimaging have attracted increasing attention. Herein, small size PEG-EuOF:(153)Sm nanocrystals (∼5 nm) (PEG = poly(ethylene glycol)bis(carboxymethyl)ether) combined with the radioactive and X-ray absorption properties were synthesized. The distribution of the PEG-EuOF nanocrystals in living animals was studied by ex vivo radioassay, inductively coupled plasma-atomic emission spectrum (ICP-AES) analysis and in vivo SPECT imaging, which indicated that the small size PEG-EuOF:(153)Sm had long blood retention time (blood half-life (t1/2) reach to 4.65 h) and were eliminated significantly through biliary/gastrointestinal pathway in vivo. Meanwhile, benefiting from the high attenuation ability of Eu, the small size PEG-EuOF was successfully applied for lymph node CT imaging, extending the bio-applications of these small nanocrystals. The results of cytotoxicity and in vivo toxicity also showed that the PEG-EuOF nanocrystals have relatively low toxicity, which suggest their safety for in vivo imaging. The studies provide preliminary validation for the use of PEG-EuOF nanocrystals for in vivo bioimaging applications.
authors.affiliation: Department of Chemistry and State Key Laboratory of Molecular Engineering of Polymers, Fudan University, Shanghai 200433, PR China.
authors.forename: Yongquan
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Chemistry and State Key Laboratory of Molecular Engineering of Polymers, Fudan University, Shanghai 200433, PR China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Chemistry and State Key Laboratory of Molecular Engineering of Polymers, Fudan University, Shanghai 200433, PR China.
authors.forename: Xingjun
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Department of Chemistry and State Key Laboratory of Molecular Engineering of Polymers, Fudan University, Shanghai 200433, PR China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Department of Chemistry and State Key Laboratory of Molecular Engineering of Polymers, Fudan University, Shanghai 200433, PR China.
authors.forename: Tianye
authors.initials: T
authors.lastname: Cao
authors.affiliation: Department of Chemistry and State Key Laboratory of Molecular Engineering of Polymers, Fudan University, Shanghai 200433, PR China.
authors.forename: Juanjuan
authors.initials: J
authors.lastname: Peng
authors.affiliation: Department of Chemistry and State Key Laboratory of Molecular Engineering of Polymers, Fudan University, Shanghai 200433, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Chemistry and State Key Laboratory of Molecular Engineering of Polymers, Fudan University, Shanghai 200433, PR China. Electronic address: fengweifd@fudan.edu.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Feng
authors.affiliation: Department of Chemistry and State Key Laboratory of Molecular Engineering of Polymers, Fudan University, Shanghai 200433, PR China. Electronic address: fyli@fudan.edu.cn.
authors.forename: Fuyou
authors.initials: F
authors.lastname: Li
mesh_terms: D000818:Animals; D003287:Contrast Media / Q000737:chemistry / Q000493:pharmacokinetics; D028581:Lanthanoid Series Elements / Q000737:chemistry / Q000493:pharmacokinetics; D008208:Lymphatic System / Q000473:pathology; D051379:Mice; D053758:Nanoparticles / Q000032:analysis / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry / Q000493:pharmacokinetics; D015899:Tomography, Emission-Computed, Single-Photon / Q000379:methods; D014057:Tomography, X-Ray Computed / Q000379:methods
chemical_list: D003287:Contrast Media; D028581:Lanthanoid Series Elements; D011092:Polyethylene Glycols
keywords: Biodistribution; Bioimaging; EuOF nanocrystals; Single photon emission computed tomography (SPECT); X-ray computed tomography (CT)