title: First isolation of reticuloendotheliosis virus from mallards in China.
abstract: Reticuloendotheliosis virus (REV) causes an oncogenic, immunosuppressive and runting syndrome in many avian hosts worldwide. REV infection has never been reported in mallard ducks, however. To identify REV infection in mallards, we collected 40 mallard duck samples from Jilin Province of China. In this study, the REV strain, DBYR1102, was first isolated from a mallard in China and identified by PCR, indirect immunofluorescence assay and electron microscopy. The gp90 gene and complete LTR of DBYR1102 were amplified and sequenced. Phylogenetic analysis based on gp90 genes of REV indicated that the REV strain DBYR1102 is closely related to strain HLJR0901 from northeastern China, the prairie chicken isolate APC-566, and REV subtype III, represented by chick syncytial virus. This new strain is distantly related to two other subtypes of REV, 170A and SNV. Phylogenetic analysis based on the LTR yielded information similar to that obtained with the gp90 genes. The results of this study not only expand our epidemiological understanding of REV in the wild birds of China but also demonstrate the potential role of wild waterfowl in REV transmission.
authors.affiliation: College of Wildlife Resources, Northeast Forestry University, Harbin, 150040, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Jiang
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Deng
authors.forename: Yulong
authors.initials: Y
authors.lastname: Gao
authors.forename: Kai
authors.initials: K
authors.lastname: Li
authors.forename: Hongliang
authors.initials: H
authors.lastname: Chai
authors.forename: Zhaobin
authors.initials: Z
authors.lastname: Fan
authors.forename: Xiangang
authors.initials: X
authors.lastname: Ren
authors.forename: Qi
authors.initials: Q
authors.lastname: Wang
authors.forename: Lizhou
authors.initials: L
authors.lastname: Zhang
authors.forename: Bingling
authors.initials: B
authors.lastname: Yun
authors.forename: Chunhong
authors.initials: C
authors.lastname: Yin
authors.forename: Yuming
authors.initials: Y
authors.lastname: Chen
authors.forename: Liting
authors.initials: L
authors.lastname: Qin
authors.forename: Honglei
authors.initials: H
authors.lastname: Gao
authors.forename: Yongqiang
authors.initials: Y
authors.lastname: Wang
authors.forename: Yuping
authors.initials: Y
authors.lastname: Hua
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Wang
mesh_terms: D000818:Animals; D046090:Anseriformes / Q000821:virology; D001715:Bird Diseases / Q000821:virology*; D005260:Female; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D001356:Reticuloendotheliosis virus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D012192:Retroviridae Infections / Q000662:veterinary* / Q000821:virology