title: Laser-induced microbubble poration of localized single cells.
abstract: Laser-induced microbubbles were used to porate the cell membranes of localized single NIH/3T3 fibroblasts. Microsecond laser pulses were focused on an optically absorbent substrate, creating a vapour microbubble that oscillated in size at the laser focal point in a fluidic chamber. The shear stress accompanying the bubble size oscillation was able to porate nearby cells. Cell poration was demonstrated with the delivery of FITC-dextran dye with various molecular weights. Under optimal poration conditions, the cell poration efficiency was up to 95.2 ± 4.8%, while maintaining 97.6 ± 2.4% cell viability. The poration system is able to target a single cell without disturbing surrounding cells.
authors.affiliation: Department of Mechanical Engineering, University of Hawaii at Manoa, 2540 Dole Street, Holmes Hall 302, Honolulu, USA. fanqihui@hawaii.edu.
authors.forename: Qihui
authors.initials: Q
authors.lastname: Fan
authors.forename: Wenqi
authors.initials: W
authors.lastname: Hu
authors.forename: Aaron T
authors.initials: AT
authors.lastname: Ohta
mesh_terms: D000818:Animals; D002470:Cell Survival; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism; D007834:Lasers; D051379:Mice; D045423:Microbubbles; D008970:Molecular Weight; D041681:NIH 3T3 Cells; D014162:Transfection / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes; D016650:Fluorescein-5-isothiocyanate