title: Monofunctional stealth nanoparticle for unbiased single molecule tracking inside living cells.
abstract: On the basis of a protein cage scaffold, we have systematically explored intracellular application of nanoparticles for single molecule studies and discovered that recognition by the autophagy machinery plays a key role for rapid metabolism in the cytosol. Intracellular stealth nanoparticles were achieved by heavy surface PEGylation. By combination with a generic approach for nanoparticle monofunctionalization, efficient labeling of intracellular proteins with high fidelity was accomplished, allowing unbiased long-term tracking of proteins in the outer mitochondrial membrane.
authors.affiliation: Department of Biology, University of Osnabrück , Osnabrück, Germany.
authors.forename: Domenik
authors.initials: D
authors.lastname: Lisse
authors.forename: Christian P
authors.initials: CP
authors.lastname: Richter
authors.forename: Christoph
authors.initials: C
authors.lastname: Drees
authors.forename: Oliver
authors.initials: O
authors.lastname: Birkholz
authors.forename: Changjiang
authors.initials: C
authors.lastname: You
authors.forename: Enrico
authors.initials: E
authors.lastname: Rampazzo
authors.forename: Jacob
authors.initials: J
authors.lastname: Piehler
mesh_terms: D001343:Autophagy; D003600:Cytosol / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000032:analysis / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000737:chemistry / Q000378:metabolism; D011506:Proteins / Q000032:analysis / Q000378:metabolism*
chemical_list: D005456:Fluorescent Dyes; D024101:Mitochondrial Proteins; D011506:Proteins; D011092:Polyethylene Glycols