title: Magnetic detection of mercuric ion using giant magnetoresistance-based biosensing system.
abstract: We have demonstrated a novel sensing strategy employing a giant magnetoresistance (GMR) biosensor and DNA chemistry for the detection of mercuric ion (Hg(2+)). This assay takes advantages of high sensitivity and real-time signal readout of GMR biosensor and high selectivity of thymine-thymine (T-T) pair for Hg(2+). The assay has a detection limit of 10 nM in both buffer and natural water, which is the maximum mercury level in drinking water regulated by U.S. Environmental Protection Agency (EPA). The magnitude of the dynamic range for Hg(2+) detection is up to three orders (10 nM to 10 μM). Herein, GMR sensing technology is first introduced into a pollutant monitoring area. It can be foreseen that the GMR biosensor could become a robust contender in the areas of environmental monitoring and food safety testing.
authors.affiliation: Department of Electrical and Computer Engineering, University of Minnesota , Minneapolis, Minnesota 55455, United States.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.forename: Liang
authors.initials: L
authors.lastname: Tu
authors.forename: Todd
authors.initials: T
authors.lastname: Klein
authors.forename: Yinglong
authors.initials: Y
authors.lastname: Feng
authors.forename: Qin
authors.initials: Q
authors.lastname: Li
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Wang
mesh_terms: D020029:Base Pairing; D015374:Biosensing Techniques / Q000379:methods*; D001710:Biotin / Q000737:chemistry; D004247:DNA / Q000737:chemistry; D060766:Drinking Water / Q000032:analysis; D059022:Food Safety; D056656:Lab-On-A-Chip Devices; D057230:Limit of Detection; D060526:Magnetic Fields; D008452:Maximum Allowable Concentration; D017669:Mercury Compounds / Q000032:analysis*; D019809:Streptavidin / Q000737:chemistry; D013941:Thymine / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000032:analysis*; D014881:Water Supply / Q000032:analysis
chemical_list: D060766:Drinking Water; D017669:Mercury Compounds; D014874:Water Pollutants, Chemical; D001710:Biotin; D004247:DNA; D019809:Streptavidin; D013941:Thymine