title: Identification of glycosylated regions in pneumococcal PspA conjugated to serotype 6B capsular polysaccharide.
abstract: Conjugate vaccines are being widely used since their introduction. Nowadays the interest in these vaccines is still growing and new antigens and conjugate chemistry are being studied and developed. Pneumococcal surface protein A (PspA) is one of the most studied pneumococcal antigens and is an important vaccine candidate. One approach to broaden the conjugate vaccine coverage could be the conjugation of the polysaccharide to a pneumococcal protein such as PspA. Previous results have shown that conjugated recombinant fragment of PspA (rPspA) not only maintained but also in some conjugates improved the induction of protective antibodies raised against the protein carrier. We describe here a characterization study to identify the domains of Streptococcus pneumoniae recombinant PspA (rPspA), from family 1 clade 1 and family 2 clade 3, involved in the conjugation with serotype 6B capsular polysaccharide.
authors.forename: Giovana Cappio
authors.initials: GC
authors.lastname: Barazzone
authors.forename: Vittoria
authors.initials: V
authors.lastname: Pinto
authors.forename: Danilo
authors.initials: D
authors.lastname: Donnarumma
authors.forename: Martha Massako
authors.initials: MM
authors.lastname: Tanizaki
authors.forename: Nathalie
authors.initials: N
authors.lastname: Norais
authors.forename: Francesco
authors.initials: F
authors.lastname: Berti
mesh_terms: D000595:Amino Acid Sequence; D016667:Bacterial Capsules / Q000276:immunology; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D006031:Glycosylation; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D022242:Pneumococcal Vaccines; D011135:Polysaccharides, Bacterial / Q000737:chemistry / Q000276:immunology*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods
chemical_list: D001426:Bacterial Proteins; D022242:Pneumococcal Vaccines; D011135:Polysaccharides, Bacterial; C051592:pneumococcal surface protein A