title: Chemical exchange in biomacromolecules: past, present, and future.
abstract: The perspective reviews quantitative investigations of chemical exchange phenomena in proteins and other biological macromolecules using NMR spectroscopy, particularly relaxation dispersion methods. The emphasis is on techniques and applications that quantify the populations, interconversion kinetics, and structural features of sparsely populated conformational states in equilibrium with a highly populated ground state. Applications to folding, molecular recognition, catalysis, and allostery by proteins and nucleic acids are highlighted.
authors.affiliation: Department of Biochemistry and Molecular Biophysics, Columbia University, 630 West 168th Street, New York, NY 10032, United States. Electronic address: agp6@columbia.edu.
authors.forename: Arthur G
authors.initials: AG
authors.lastname: Palmer
mesh_terms: D000465:Algorithms; D000818:Animals; D019295:Computational Biology; D006801:Humans; D046911:Macromolecular Substances / Q000737:chemistry*; D008958:Models, Molecular; D008968:Molecular Conformation; D019906:Nuclear Magnetic Resonance, Biomolecular; D009696:Nucleic Acids / Q000737:chemistry
chemical_list: D046911:Macromolecular Substances; D009696:Nucleic Acids
keywords: Dynamics; Kinetics; NMR; Nucleic acids; Proteins; Relaxation