title: The effect of different polychlorinated biphenyls on two aquatic models, the green alga Pseudokirchneriella subcapitata and the haemocytes from the European abalone Haliotis tuberculata.
abstract: The present study was conducted to determine the toxicity of different polychlorinated biphenyls (PCBs) on the green algae, Pseudokirchneriella subcapitata and the haemocytes from the European abalone, Haliotis tuberculata. Using the algal growth inhibition test, the green algae median Effective Concentration (EC50) values ranged from 0.34μM for PCB28 to more than 100μM for PCBs 101 and 153. Considering the MTT viability test, the abalone EC50 values ranged from 1.67μM for PCB153 to 89μM for PCB28. Our results in contrast to previous observation in vertebrates did not show significant differences between the dioxin like- and non dioxin like-PCBs toxicities regardless of the model used. However, our results demonstrated that the toxicities of PCBs were species dependent. For example, PCB28 was the most toxic compound for P. subcapitata whereas PCBs 1, 180 and 153 were less toxic for that species. On the contrary, PCB153 was reported as the most toxic for H. tuberculata haemocytes and PCB28 the least toxic. To investigate the mode of action of these compounds, we used an in silico method. Our results suggested that PCBs have a non-specific mode of action (e.g., narcosis) on green algae, and another mode of action, probably more specific than narcosis, was reported for PCBs on the abalone haemocytes.
authors.affiliation: Normandie Université, F-14032 Caen, France; Centre d'Etudes et de Recherche sur le Médicament de Normandie, UPRES EA-4258, INC3M FR CNRS 3038, SFR ICORE, Université de Caen Basse-Normandie, UFR des Sciences pharmaceutiques, Boulevard Becquerel, F-14032 Caen cedex, France. Electronic address: marie-pierre.halm@unicaen.fr.
authors.forename: Marie-Pierre
authors.initials: MP
authors.lastname: Halm-Lemeille
authors.affiliation: Normandie Université, F-14032 Caen, France; Centre d'Etudes et de Recherche sur le Médicament de Normandie, UPRES EA-4258, INC3M FR CNRS 3038, SFR ICORE, Université de Caen Basse-Normandie, UFR des Sciences pharmaceutiques, Boulevard Becquerel, F-14032 Caen cedex, France; CNRS INEE, FRE3484 BioMEA, SFR ICORE, IBFA Université de Caen Basse-Normandie, IBFA, Esplanade de la Paix, F-14032 Caen, France.
authors.forename: Elham
authors.initials: E
authors.lastname: Abbaszadeh Fard
authors.affiliation: Normandie Université, F-14032 Caen, France; CNRS INEE, FRE3484 BioMEA, SFR ICORE, IBFA Université de Caen Basse-Normandie, IBFA, Esplanade de la Paix, F-14032 Caen, France.
authors.forename: Thomas
authors.initials: T
authors.lastname: Latire
authors.affiliation: Université de Lorraine, Laboratoire Interdisciplinaire des Environnements Continentaux (LIEC), UMR 7360 CNRS, Rue du Général Delestraint, F-57070 Metz, France.
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Ferard
authors.affiliation: Normandie Université, F-14032 Caen, France; CNRS INEE, FRE3484 BioMEA, SFR ICORE, IBFA Université de Caen Basse-Normandie, IBFA, Esplanade de la Paix, F-14032 Caen, France.
authors.forename: Katherine
authors.initials: K
authors.lastname: Costil
authors.affiliation: Normandie Université, F-14032 Caen, France; CNRS INEE, FRE3484 BioMEA, SFR ICORE, IBFA Université de Caen Basse-Normandie, IBFA, Esplanade de la Paix, F-14032 Caen, France.
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Lebel
authors.affiliation: Normandie Université, F-14032 Caen, France; Centre d'Etudes et de Recherche sur le Médicament de Normandie, UPRES EA-4258, INC3M FR CNRS 3038, SFR ICORE, Université de Caen Basse-Normandie, UFR des Sciences pharmaceutiques, Boulevard Becquerel, F-14032 Caen cedex, France.
authors.forename: Ronan
authors.initials: R
authors.lastname: Bureau
authors.affiliation: Normandie Université, F-14032 Caen, France; CNRS INEE, FRE3484 BioMEA, SFR ICORE, IBFA Université de Caen Basse-Normandie, IBFA, Esplanade de la Paix, F-14032 Caen, France. Electronic address: antoine.serpentini@unicaen.fr.
authors.forename: Antoine
authors.initials: A
authors.lastname: Serpentini
mesh_terms: D000818:Animals; D000460:Chlorophyta / Q000187:drug effects* / Q000502:physiology; D004785:Environmental Pollutants / Q000633:toxicity*; D049851:Gastropoda / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D006434:Hemocytes / Q000166:cytology / Q000187:drug effects*; D011078:Polychlorinated Biphenyls / Q000633:toxicity*; D000072317:Polychlorinated Dibenzodioxins
chemical_list: D004785:Environmental Pollutants; D000072317:Polychlorinated Dibenzodioxins; D011078:Polychlorinated Biphenyls
keywords: Haemocyte; Haliotis tuberculata; Mechanism of action; Polychlorinated biphenyls; Pseudokirchneriella subcapitata