title: Osteoconductive bio-based meshes based on poly(hydroxybutyrate-co-hydroxyvalerate) and poly(butylene adipate-co-terephthalate) blends.
abstract: Poly(butylene adipate-co-terephthalate) (PBAT) and Poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) are biopolymers that have the potential to be used in applications of bone healing. In this study, it is hypothesized that the polymer blend has the combined strength and osteoconductivity to support osteoblast collagen formation. PBAT (PBAT 100), and a blend with 20% PHBV (PBAT 80) were extruded in the form of fibers and then knitted in the form of mesh. These were tested in the warp as well as weft direction for the tensile properties; these showed that the weft direction had higher performance than the warp. The individual fibers were kept in phosphate buffered saline (PBS) over the period of 8 weeks and were tested for the storage and loss modulus using a dynamic mechanical analyser (DMA). The results indicated that mechanical relaxation strength showed a decrease and then an increase. In vitro osteoconductivity studies were done by using differentiating osteoblasts (MC3T3-E1 subclone 4 cells). Environmental Scanning Electron Microscopy (ESEM) showed that pre-soaking the samples in α-MEM for two weeks resulted in cell attachment and growth. X-ray diffraction (XRD) was used to determine the change in structure of polymers due to in vitro degradation for two weeks. Raman spectroscopy showed that all scaffolds supported the formation of a collagenous network over the scaffold surfaces. For a combination of knittable manufacturing, mechanical performance and osteoconductivity, blends offer an effective route.
authors.affiliation: Department of Material Science and Engineering, University of North Texas, 1155 Union Circle #305310, Denton, TX 76203-5017, United States.
authors.forename: Mangesh
authors.initials: M
authors.lastname: Nar
authors.affiliation: Department of Material Science and Engineering, University of North Texas, 1155 Union Circle #305310, Denton, TX 76203-5017, United States.
authors.forename: Gerrit
authors.initials: G
authors.lastname: Staufenberg
authors.affiliation: Department of Material Science and Engineering, University of North Texas, 1155 Union Circle #305310, Denton, TX 76203-5017, United States.
authors.forename: Bing
authors.initials: B
authors.lastname: Yang
authors.affiliation: Department of Fibers, College of Visual Arts and Design, 1155 Union Circle #305100, Denton, TX 76203-5017, United States.
authors.forename: Lesli
authors.initials: L
authors.lastname: Robertson
authors.affiliation: Department of Biomedical Sciences and Center for Craniofacial Research and Diagnosis, Texas A&M University Baylor College of Dentistry, Dallas, TX 75246, United States.
authors.forename: Rinkesh H
authors.initials: RH
authors.lastname: Patel
authors.affiliation: Department of Biomedical Sciences and Center for Craniofacial Research and Diagnosis, Texas A&M University Baylor College of Dentistry, Dallas, TX 75246, United States. Electronic address: vvaranasi@bcd.tamhsc.edu.
authors.forename: Venu G
authors.initials: VG
authors.lastname: Varanasi
authors.affiliation: Department of Material Science and Engineering, University of North Texas, 1155 Union Circle #305310, Denton, TX 76203-5017, United States; Department of Mechanical and Energy Engineering, University of North Texas, 1155 Union Circle #311098, Denton, TX 76203-5017, United States. Electronic address: nandika.dsouza@unt.edu.
authors.forename: Nandika Anne
authors.initials: NA
authors.lastname: D'Souza
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D002152:Calorimetry, Differential Scanning; D002460:Cell Line; D055119:Elastic Modulus / Q000187:drug effects; D051379:Mice; D008855:Microscopy, Electron, Scanning; D016348:Osseointegration / Q000187:drug effects*; D011091:Polyesters / Q000494:pharmacology*; D013059:Spectrum Analysis, Raman; D013314:Stress, Mechanical; D013718:Tensile Strength / Q000187:drug effects; D014961:X-Ray Diffraction; D000094122:Polyhydroxybutyrates
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; C052620:poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); D000094122:Polyhydroxybutyrates; C488797:poly(butylene adipate-co-butylene terephthalate)
keywords: Collagen formation; Degradation; Osteoblasts; PBAT; PHBV