title: Role of dorsal vagal complex A2 noradrenergic neurons in hindbrain glucoprivic inhibition of the luteinizing hormone surge in the steroid-primed ovariectomized female rat: effects of 5-thioglucose on A2 functional biomarker and AMPK activity.
abstract: Neuro-glucostasis is required for normal expression of the steroid positive-feedback-induced preovulatory pituitary luteinizing hormone (LH) surge, a critical element of female reproduction. Glucoprivic signals from the caudal hindbrain restrain this surge, but the cellular source of this stimulus is unclear. Norepinephrine (NE) exerts well-defined stimulatory effects on the reproductive neuroendocrine axis. Our studies show that medullary A2 noradrenergic neurons are both estrogen- and glucoprivic-sensitive. Here, we investigated the premise that the LH surge is inhibited by A2 cell reactivity to hindbrain glucopenia and diminished preoptic NE neurotransmission. Estradiol- and progesterone-primed ovariectomized (OVX) female rats were injected into the caudal fourth ventricle (CV4) with the glucose anti-metabolite, 5-thioglucose (5TG) or saline (SAL) prior to onset of the LH surge. Pretreatment by intra-CV4 delivery of the selective catecholamine neurotoxin, 6-OHDA, attenuated LH output, but prevented inhibition by 5TG. 5TG modified patterns of steroid feedback-associated Fos staining of A2, but not other medullary catecholamine cell groups. Intra-preoptic administration of the alpha₁-adrenergic receptor agonist, methoxamine, elicited site-specific reversal of hindbrain glucoprivic suppression of gonadotropin-releasing hormone (GnRH) neuron Fos labeling and LH release. Western blotting of laser-microdissected A2 neurons revealed glucoprivic stimulation of Fos, but inhibition of the catecholamine synthetic enzyme, dopamine-β-hydroxylase; 5TG also diminished A2 estrogen receptor (ER)-α and progesterone receptor profiles, but augmented ER-β protein. Intriguingly, A2 AMPK activity was decreased in 5TG-treated rats, despite down-regulation of GLUT3 and no change in MCT2 protein expression. Rostral preoptic GnRH neurons also exhibited decreased AMPK activation simultaneous with apparent reduction of neuropeptide signaling to the pituitary. The present studies demonstrate that hindbrain glucoprivation inhibits the LH surge, in part, by reducing preoptic noradrenergic input, and furthermore implicate A2 neurons as a source of this altered signal. Results also suggest that AMPK sensor deactivation does not supersede the impact of pharmacological inhibition of glucose catabolism on A2 cell function nor afferent signaling of hindbrain glucopenia on GnRH neurons. Further studies are needed to determine if decreased AMPK activation in these cell populations reflect compensatory gain in positive energy balance and/or direct effects of estrogen on AMPK.
authors.affiliation: Department of Basic Pharmaceutical Sciences, School of Pharmacy, The University of Louisiana at Monroe, Monroe, LA 71201, USA.
authors.forename: B A
authors.initials: BA
authors.lastname: Ibrahim
authors.affiliation: Department of Basic Pharmaceutical Sciences, School of Pharmacy, The University of Louisiana at Monroe, Monroe, LA 71201, USA. Electronic address: briski@ulm.edu.
authors.forename: K P
authors.initials: KP
authors.lastname: Briski
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000378:metabolism; D018663:Adrenergic Agents / Q000494:pharmacology; D059331:Adrenergic Neurons / Q000187:drug effects / Q000502:physiology*; D058646:Adrenergic alpha-1 Receptor Agonists / Q000494:pharmacology; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002491:Central Nervous System Agents / Q000494:pharmacology; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D005947:Glucose / Q000031:analogs & derivatives / Q000494:pharmacology; D007987:Gonadotropin-Releasing Hormone / Q000378:metabolism; D006728:Hormones / Q000494:pharmacology; D007986:Luteinizing Hormone / Q000378:metabolism*; D008729:Methoxamine / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects / Q000502:physiology; D010052:Ovariectomy; D016627:Oxidopamine / Q000494:pharmacology; D011374:Progesterone / Q000494:pharmacology; D017207:Rats, Sprague-Dawley; D043683:Receptors, Adenosine A2 / Q000378:metabolism*; D012249:Rhombencephalon / Q000187:drug effects / Q000502:physiology*
chemical_list: D018663:Adrenergic Agents; D058646:Adrenergic alpha-1 Receptor Agonists; D015415:Biomarkers; D002491:Central Nervous System Agents; D006728:Hormones; D043683:Receptors, Adenosine A2; D007987:Gonadotropin-Releasing Hormone; C003002:5-thio-D-glucose; D011374:Progesterone; D004958:Estradiol; D016627:Oxidopamine; D007986:Luteinizing Hormone; D055372:AMP-Activated Protein Kinases; D008729:Methoxamine; D005947:Glucose
keywords: 5-thioglucose; A2 noradrenergic neurons; AMPK; estrogen receptor; gonadotropin-releasing hormone