title: Immobilized N-chlorosuccinimide as a friendly peptide disulfide-forming reagent.
abstract: A novel immobilized N-chlorosuccinimide resin was developed for peptide disulfide bond formation in combinatorial libraries. The resin is prepared in a simple two-step process from commercial starting materials. Disulfide formation is initiated by adding a peptide solution to the resin, and excess reagent is removed by a convenient filtration upon completion of disulfide formation. Completion of disulfide formation is rapid and clean, as demonstrated by the oxidation of a small nonapeptide library. This immobilized reagent allows a wider scope for the use of N-chlorosuccinimide-based disulfide formation in combinatorial chemistry.
authors.affiliation: Institute for Research in Biomedicine (IRB) Barcelona , Baldiri Reixac 10, Barcelona 08028, Spain.
authors.forename: Tobias M
authors.initials: TM
authors.lastname: Postma
authors.forename: Fernando
authors.initials: F
authors.lastname: Albericio
mesh_terms: D004220:Disulfides / Q000138:chemical synthesis* / Q000737:chemistry; D015394:Molecular Structure; D010455:Peptides / Q000737:chemistry*; D012117:Resins, Synthetic / Q000138:chemical synthesis / Q000737:chemistry*; D013388:Succinimides / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D004220:Disulfides; D010455:Peptides; D012117:Resins, Synthetic; D013388:Succinimides; C015752:N-chlorosuccinimide