title: One-pot green synthesis of carbon dots by using Saccharum officinarum juice for fluorescent imaging of bacteria (Escherichia coli) and yeast (Saccharomyces cerevisiae) cells.
abstract: We are reporting highly economical plant-based hydrothermal method for one-pot green synthesis of water-dispersible fluorescent carbon dots (CDs) by using Saccharum officinarum juice as precursor. The synthesized CDs were characterized by UV-visible, fluorescence, Fourier transform infrared (FT-IR), dynamic light scattering (DLS), high-resolution transmission electron microscopic (HR-TEM), and laser scanning confocal microscopic techniques. The CDs are well dispersed in water with an average size of ~3 nm and showed bright blue fluorescence under UV-light (λex=365 nm). These CDs acted as excellent fluorescent probes in cellular imaging of bacteria (Escherichia coli) and yeast (Saccharomyces cerevisiae).
authors.affiliation: Applied Chemistry Department, S. V. National Institute of Technology, Surat, 395 007, India.
authors.forename: Vaibhavkumar N
authors.initials: VN
authors.lastname: Mehta
authors.affiliation: Gujarat Agricultural Biotechnology Institute, Navsari Agricultural University, Surat, 395007, India.
authors.forename: Sanjay
authors.initials: S
authors.lastname: Jha
authors.affiliation: Applied Chemistry Department, S. V. National Institute of Technology, Surat, 395 007, India. Electronic address: sureshkumarchem@gmail.com.
authors.forename: Suresh Kumar
authors.initials: SK
authors.lastname: Kailasa
mesh_terms: D001628:Beverages; D002244:Carbon / Q000737:chemistry*; D004926:Escherichia coli / Q000166:cytology*; D055772:Green Chemistry Technology / Q000379:methods*; D050296:Microbial Viability; D046529:Microscopy, Electron, Transmission; D012441:Saccharomyces cerevisiae / Q000166:cytology*; D031786:Saccharum / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D014961:X-Ray Diffraction
chemical_list: D002244:Carbon
keywords: Bacteria and yeast cells; DLS; Fluorescence microscopy; Saccharum officinarum juice; TEM