title: [Formation and identification of virus-like particles of poliovirus type I].
abstract: OBJECTIVE
  To establish a method to produce virus-like particles (VLP) of poliovirus type I in Saccharomy cescerevisiae to develop potential novel recombinant vaccine against poliovirus type 1.
  
  
  METHODS
  The genes of P1 and 3CD of poliovirus type I were optimized, synthesized and inserted into expression vector, which was further transfected into Saccharomy cescerevisiae. The extracts of yeast cells were purified by CsCl density gradient centrifugation after induction and cell lysis.
  
  
  RESULTS
  Electrophoresis and sequencing analyses showed that the genes P1 and 3CD of poliovirus type I were successfully inserted into expression vector and encode a protein whose amino acid sequences were identical with wide-type genes of poliovirus type I. Electronic microscopy analysis showed that the VLPs of poliovirus type I could be efficiently formed in Saccharomy cescerevisiae.
  
  
  CONCLUSIONS
  The VLPs of poliovirus type I could be efficiently produced by co-expression of P1 and 3CD genes in Saccharomy cescerevisiae.
authors.forename: Xiao-Wen
authors.initials: XW
authors.lastname: Wang
authors.forename: Wang
authors.initials: W
authors.lastname: Sheng
authors.forename: Yi
authors.initials: Y
authors.lastname: Zeng
mesh_terms: D005260:Female; D015870:Gene Expression; D006801:Humans; D008297:Male; D011051:Poliomyelitis / Q000517:prevention & control / Q000821:virology*; D017955:Poliovirus / Q000235:genetics* / Q000378:metabolism; D023321:Poliovirus Vaccines / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism; D014771:Virion / Q000235:genetics* / Q000378:metabolism
chemical_list: D023321:Poliovirus Vaccines; D014764:Viral Proteins