title: Maize IgE binding proteins: each plant a different profile?
abstract: BACKGROUND
  Allergies are nearly always triggered by protein molecules and the majority of individuals with documented immunologic reactions to foods exhibit IgE hypersensitivity reactions. In this study we aimed to understand if natural differences, at proteomic level, between maize populations, may induce different IgE binding proteins profiles among maize-allergic individuals. We also intended to deepen our knowledge on maize IgE binding proteins.
  
  
  RESULTS
  In order to accomplish this goal we have used proteomic tools (SDS-PAGE and 2-D gel electrophoresis followed by western blot) and tested plasma IgE reactivity from four maize-allergic individuals against four different protein fractions (albumins, globulins, glutelins and prolamins) of three different maize cultivars. We have observed that maize cultivars have different proteomes that result in different IgE binding proteins profiles when tested against plasma from maize-allergic individuals. We could identify 19 different maize IgE binding proteins, 11 of which were unknown to date. Moreover, we found that most (89.5%) of the 19 identified potential maize allergens could be related to plant stress.
  
  
  CONCLUSIONS
  These results lead us to conclude that, within each species, plant allergenic potential varies with genotype. Moreover, considering the stress-related IgE binding proteins identified, we hypothesise that the environment, particularly stress conditions, may alter IgE binding protein profiles of plant components.
authors.forename: Cátia
authors.initials: C
authors.lastname: Fonseca
authors.forename: Sébastien
authors.initials: S
authors.lastname: Planchon
authors.forename: Carla
authors.initials: C
authors.lastname: Pinheiro
authors.forename: Jenny
authors.initials: J
authors.lastname: Renaut
authors.forename: Cândido Pinto
authors.initials: CP
authors.lastname: Ricardo
authors.forename: M Margarida
authors.initials: MM
authors.lastname: Oliveira
authors.affiliation: National Health Institute Dr, Ricardo Jorge, Av, Padre Cruz, 1649-016 Lisboa, Portugal. rita.batista@insa.min-saude.pt.
authors.forename: Rita
authors.initials: R
authors.lastname: Batista