title: Molecular RHD screening of RhD negative donors can replace standard serological testing for RhD negative donors.
abstract: This work aims to assess the value of a generalized molecular RHD screening strategy which could replace routine serological screening of weak D by indirect antiglobulin test. Three independent studies were performed at the two Blood Transfusion Services Berne and Zurich. Donors investigated were 652 RhD negative, but RhC and/or RhE positive, 17,391 mainly Rhccee, and 8200 with normal RhCcEe phenotype distribution. In study I single samples, in studies II and III minipools of 24 and 20 donor samples were tested, respectively. Among 26,243 phenotypically RhD negative blood donors, 65 carriers of RHD alleles were identified. Thirty-one of them were redefined as RhD positive.
authors.affiliation: Blood Transfusion Service SRC Berne, Bern, Switzerland.
authors.forename: Peter
authors.initials: P
authors.lastname: Gowland
authors.affiliation: Blood Transfusion Service SRC Zurich, Zurich, Switzerland.
authors.forename: Christoph
authors.initials: C
authors.lastname: Gassner
authors.affiliation: Blood Transfusion Service SRC Berne, Bern, Switzerland.
authors.forename: Hein
authors.initials: H
authors.lastname: Hustinx
authors.affiliation: Blood Transfusion Service SRC Berne, Bern, Switzerland.
authors.forename: Martin
authors.initials: M
authors.lastname: Stolz
authors.affiliation: Blood Transfusion Service SRC Zurich, Zurich, Switzerland.
authors.forename: Jochen
authors.initials: J
authors.lastname: Gottschalk
authors.affiliation: Blood Transfusion Service SRC Vaud, Epalinges, Switzerland.
authors.forename: Jean-Daniel
authors.initials: JD
authors.lastname: Tissot
authors.affiliation: Blood Transfusion Service SRC Nordost Schweiz, St. Gallen, Switzerland.
authors.forename: Jutta
authors.initials: J
authors.lastname: Thierbach
authors.affiliation: Blood Transfusion Service SRC Innerschweiz, Luzern, Switzerland.
authors.forename: Andreas
authors.initials: A
authors.lastname: Maier
authors.affiliation: Blood Transfusion Service SRC Zurich, Zurich, Switzerland.
authors.forename: Sonja
authors.initials: S
authors.lastname: Sigurdardottir
authors.affiliation: Blood Transfusion Service SRC Berne, Bern, Switzerland.
authors.forename: Franziska
authors.initials: F
authors.lastname: Still
authors.affiliation: Blood Transfusion Service SRC Berne, Bern, Switzerland.
authors.forename: Stefano
authors.initials: S
authors.lastname: Fontana
authors.affiliation: Blood Transfusion Service SRC Zurich, Zurich, Switzerland. Electronic address: bm.frey@zhbsd.ch.
authors.forename: Beat M
authors.initials: BM
authors.lastname: Frey
authors.affiliation: Blood Transfusion Service SRC Berne, Bern, Switzerland. Electronic address: christoph.niederhauser@bsd-be.ch.
authors.forename: Christoph
authors.initials: C
authors.lastname: Niederhauser
mesh_terms: D000483:Alleles; D001788:Blood Grouping and Crossmatching / Q000379:methods*; D046148:Donor Selection / Q000379:methods*; D005260:Female; D060005:Genotyping Techniques / Q000379:methods*; D006801:Humans; D008297:Male; D010641:Phenotype; D012204:Rh-Hr Blood-Group System / Q000235:genetics*
chemical_list: D012204:Rh-Hr Blood-Group System; C026410:Rho(D) antigen
keywords: IAT; Molecular RHD screening; RhD