title: Exercise pretreatment promotes mitochondrial dynamic protein OPA1 expression after cerebral ischemia in rats.
abstract: Exercise training is a neuroprotective strategy in cerebral ischemic injury, but the underlying mechanisms are not yet clear. In the present study, we investigated the effects of treadmill exercise pretreatment on the expression of mitochondrial dynamic proteins. We examined the expression of OPA1/DLP1/MFF/Mfn1/Mfn2, which regulates mitochondrial fusion and fission, and cytochrome C oxidase subunits (COX subunits), which regulate mitochondrial functions, after middle cerebral artery occlusion (MCAO) in rats. T2-weighted magnetic resonance imaging (MRI) was evaluated as indices of brain edema after ischemia as well. Treadmill training pretreatment increased the expression levels of OPA1 and COXII/III/IV and alleviated brain edema, indicating that exercise pretreatment provided neuroprotection in cerebral ischemic injury via the regulation of mitochondrial dynamics and functions.
authors.affiliation: Department of Rehabilitation, Huashan Hospital, Fudan University, Shanghai 200040, China. lenzhangli@gmail.com.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Rehabilitation, Huashan Hospital, Fudan University, Shanghai 200040, China. he_zhijie@hotmail.com.
authors.forename: Zhijie
authors.initials: Z
authors.lastname: He
authors.affiliation: Department of Rehabilitation, Huashan Hospital, Fudan University, Shanghai 200040, China. friday0451@163.com.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Rehabilitation, Huashan Hospital, Fudan University, Shanghai 200040, China. wuyi4000@163.com.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Rehabilitation, Huashan Hospital, Fudan University, Shanghai 200040, China. xj8842436@163.com.
authors.forename: Xiaojiao
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Rehabilitation, Huashan Hospital, Fudan University, Shanghai 200040, China. wx_niu@126.com.
authors.forename: Wenxiu
authors.initials: W
authors.lastname: Niu
authors.affiliation: Department of Rehabilitation, Huashan Hospital, Fudan University, Shanghai 200040, China. drhuys@sina.com.cn.
authors.forename: Yongshan
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Department of Rehabilitation, Huashan Hospital, Fudan University, Shanghai 200040, China. shannonjj@126.com.
authors.forename: Jie
authors.initials: J
authors.lastname: Jia
mesh_terms: D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000098:blood supply / Q000378:metabolism / Q000473:pathology; D002545:Brain Ischemia / Q000209:etiology / Q000378:metabolism* / Q000503:physiopathology; D034281:Dynamins / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000378:metabolism; D020558:GTP Phosphohydrolases / Q000378:metabolism*; D020244:Infarction, Middle Cerebral Artery / Q000150:complications / Q000378:metabolism / Q000503:physiopathology; D008279:Magnetic Resonance Imaging; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D063154:Mitochondrial Dynamics / Q000502:physiology; D024101:Mitochondrial Proteins / Q000378:metabolism*; D010805:Physical Conditioning, Animal / Q000502:physiology*; D017207:Rats, Sprague-Dawley; D013997:Time Factors
chemical_list: D008565:Membrane Proteins; C489709:Mfn1 protein, rat; D024101:Mitochondrial Proteins; D003576:Electron Transport Complex IV; D020558:GTP Phosphohydrolases; C489710:Mfn2 protein, rat; C498811:Opa1 protein, rat; C519851:Dnm1l protein, rat; D034281:Dynamins