title: GABA in the nervous system of the cestodes Diphyllobothrium dendriticum (Diphyllobothriidea) and Caryophyllaeus laticeps (Caryophyllidea), with comparative analysis of muscle innervation.
abstract: The nervous system (NS) of the cestodes Diphyllobothrium dendriticum (Diphyllobothriidea) and Caryophyllaeus laticeps (Caryophyllidea) was investigated using immunocytochemistry. The GABA neurotransmitter was identified in the NS of both species; GABAergic neurons were detected in the main nerve cords (MC). GABA-like immunoreactive neurons were predominantly unipolar and exhibited more intensive immunoreactivity in the neurite than in the perikaryon. In C. laticeps , GABA-like immunoreactive somas are located in both the MCs and peripheral NS near the longitudinal muscles. The innervation of the body musculature was studied using a combination of antibodies against GABA, serotonin (5-HT), and FMRFamide and with complementary staining of F-actin. In both species, the location of GABAergic neurites is associated with all muscle layers including the subtegumental, longitudinal, transverse, and dorsoventral muscles. The cytomorphology of 5-HTergic motoneurons in the MCs of both species is described and differences in muscle innervation between D. dendriticum and C. laticeps are demonstrated. No evidence for co-localization of GABA with 5-HT or FMRFamide neurotransmitters at any particular neuron was found. Neuropiles in MCs and peripheral NS had separate sets of immunoreactive neurites. A functional role for GABA in muscle innervation is discussed.
authors.affiliation: Biological Faculty, M.V. Lomonosov Moscow State University, 1/12, Leninskie Gory, 119991 Moscow, Russia.
authors.forename: Natalia M
authors.initials: NM
authors.lastname: Biserova
authors.forename: Ivan A
authors.initials: IA
authors.lastname: Kutyrev
authors.forename: Kirsten
authors.initials: K
authors.lastname: Jensen
mesh_terms: D000818:Animals; D002589:Cestoda / Q000737:chemistry / Q000502:physiology* / Q000648:ultrastructure; D004170:Diphyllobothrium / Q000737:chemistry / Q000502:physiology / Q000648:ultrastructure; D019835:FMRFamide / Q000032:analysis; D005629:Frozen Sections; D059330:GABAergic Neurons / Q000737:chemistry / Q000502:physiology*; D007091:Image Processing, Computer-Assisted; D007106:Immune Sera / Q000276:immunology; D007150:Immunohistochemistry; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D009132:Muscles / Q000294:innervation; D009420:Nervous System / Q000737:chemistry; D010590:Phalloidine; D059326:Serotonergic Neurons / Q000737:chemistry / Q000502:physiology; D012701:Serotonin / Q000032:analysis; D005680:gamma-Aminobutyric Acid / Q000032:analysis* / Q000276:immunology / Q000502:physiology
chemical_list: D007106:Immune Sera; D010590:Phalloidine; D012701:Serotonin; D005680:gamma-Aminobutyric Acid; D019835:FMRFamide