title: Detection of Anaplasma phagocytophilum and Anaplasma bovis in small wild mammals from Taichung and Kinmen Island, Taiwan.
abstract: A total of 138 animals from 7 species (Apodemus agrarius, Bandicota indica, Crocidura suaveolens, Mus caroli, Mus formosanus, Rattus losea, and Suncus murinus) captured in Taichung, located in central Taiwan, and Kinmen Island, an island off the shore of China, were examined for the presence of Anaplasma phagocytophilum. The presence of the bacteria, which causes human granulocytic anaplasmosis, was examined by nested PCR targeting the16S rDNA. Twelve animals (8.7%) from M. caroli and R. losea, and 25 (18.1%) from A. agrarius, B. indica, M. caroli, and R. losea were infected with A. phagocytophilum and Anaplasma bovis, respectively. Phylogenetic analysis revealed that partial 16S rDNA sequences in the 12 aforementioned animals showed higher similarity to the sequences related to A. phagocytophilum detected in wild rodents (Rattus and Niviventer) from southeast China. The sequences of the other 25 animals belonged to the A. bovis clade. We demonstrated that small wild mammals were infected with A. phagocytophilum and A. bovis in Taichung and Kinmen Island, Taiwan.
authors.affiliation: Laboratory of Microbiology and Immunology, Faculty of Pharmacy, Chiba Institute of Science (CIS).
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Masuzawa
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Uchishima
authors.forename: Takashi
authors.initials: T
authors.lastname: Fukui
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Okamoto
authors.forename: Ming-Jeng
authors.initials: MJ
authors.lastname: Pan
authors.forename: Teruki
authors.initials: T
authors.lastname: Kadosaka
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Takada
mesh_terms: D041081:Anaplasma phagocytophilum / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D000818:Animals; D000835:Animals, Wild / Q000382:microbiology*; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D016873:Ehrlichiosis / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D008322:Mammals; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013624:Taiwan / Q000453:epidemiology
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S