title: [Antigenic analysis of two chimeric hepatitis B core particles presenting the preS1 neutralizing epitopes].
abstract: OBJECTIVE
  To construct full-length hepatitis B core particles presenting preS1 aa 21-47 epitope and truncated core particles presenting preS1 aa 37-45 epitope on their surface and compare their antigenicity.
  
  
  METHODS
  PreS1 aa21-47 epitope and aa 37-45 epitope were inserted respectively into full-length hepatitis B core (aa 1-183) and truncated HBcAg (aa 1-144), between the 78th (Asp) and 79th (Pro). The genes synthesized after the codon optimization were ligated to the pET43. 1a vector with the same cohesive terminal (NdeI and XhoI) and expressed in the E. coli expression system. The morphology of the proteins of interest were observed by electron microscope and characterized by ELISA and Western Blotting.
  
  
  RESULTS
  The morphology of the virus-like particles were confirmed by electron microscope. H2 were solid particles with a diameter of (31.61 +/- 1.27) nm, while H3 were hollow particles with a diameter of (28.46 +/- 1.16) nm. Statistical analysis showed that H2 is larger than H3 in the diameter (P < 0.01). The antigenicity of the inserted epitopes and carrier protein were identified by ELISA and Western Blotting.
  
  
  CONCLUSIONS
  Chimeric hepatitis B core particles presenting the preS1 neutralizing epitopes on their surface have been expressed, purified and identified, which lays the foundation for its application in vaccine research.
authors.forename: Qin-Dong
authors.initials: QD
authors.lastname: Su
authors.forename: Min-Zhuo
authors.initials: MZ
authors.lastname: Guo
authors.forename: Yao
authors.initials: Y
authors.lastname: Yi
authors.forename: Si-Yong
authors.initials: SY
authors.lastname: Chen
authors.forename: Zhi-Yuan
authors.initials: ZY
authors.lastname: Jia
authors.forename: Xue-Xin
authors.initials: XX
authors.lastname: Lu
authors.forename: Feng
authors.initials: F
authors.lastname: Qiu
authors.forename: Sheng-Li
authors.initials: SL
authors.lastname: Bi
mesh_terms: D000939:Epitopes / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D006509:Hepatitis B / Q000276:immunology* / Q000821:virology; D006512:Hepatitis B Core Antigens / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D006514:Hepatitis B Surface Antigens / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D006515:Hepatitis B virus / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D006801:Humans; D009500:Neutralization Tests; D011498:Protein Precursors / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology
chemical_list: D000939:Epitopes; D006512:Hepatitis B Core Antigens; D006514:Hepatitis B Surface Antigens; D011498:Protein Precursors; D011993:Recombinant Fusion Proteins; C050806:presurface protein 1, hepatitis B surface antigen