title: Alkaloids from Lycoris aurea and their cytotoxicities against the head and neck squamous cell carcinoma.
abstract: Phytochemical investigation of the 80% EtOH extract of the bulbs of Lycoris aurea led to the isolation of six new alkaloids, 2-demethyl-isocorydione (1), 8-demethyl-dehydrocrebanine (2), 1-hydroxy-anhydrolycorin-7-one (3), (+)-1,2-dihydroxy-anhydrolycorine N-oxide (4), 5,6-dihydro-5-methyl-2-hydroxyphenanthridine (5), and (+)-8-hydroxy-homolycorine-α-N-oxide (6), and together with two known compounds, isocorydione (7) and anhydrolycorin-7-one (8). Structural elucidation of all the compounds was performed by spectral methods such as 1D and 2D ((1)H-(1)H COSY, HMQC, and HMBC) NMR spectroscopy, in addition to high resolution mass spectrometry. All the alkaloids were in vitro evaluated for their cytotoxic activities against seven tumor cell lines of the head and neck squamous cell carcinoma and anti-inflammatory activities. Compounds 1, 2, 6, and 7 exhibited significant cytotoxicities against all the tested cell lines. Moreover, alkaloids 1, 2, and 7 possessed selective inhibition of Cox-2 (>85%).
authors.affiliation: Department of Otolaryngology, Shenyang 463 Hospital, Xiao He Yan Road 46, Shenyang, Liaoning 110042 China.
authors.forename: Jin-Hui
authors.initials: JH
authors.lastname: Song
authors.affiliation: Department of Oral and Maxillofacial Surgery, General Hospital of Shenyang Military Area Command, No.83, Wenhua Road, Shenhe District, Shenyang, Liaoning 110840 China. Electronic address: lzhangsy@sohu.com.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Pharmacy, 455 Hospital of People's Liberation Army, West Huaihai Road 338, Shanghai 200052 China. Electronic address: songyan455@gmail.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Song
mesh_terms: D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification / Q000627:therapeutic use*; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification / Q000627:therapeutic use; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000627:therapeutic use*; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D006258:Head and Neck Neoplasms / Q000188:drug therapy*; D006801:Humans; D031426:Lycoris / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008516:Medicine, Chinese Traditional; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000627:therapeutic use*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal
chemical_list: D000470:Alkaloids; D000893:Anti-Inflammatory Agents; D000970:Antineoplastic Agents; D010936:Plant Extracts
keywords: Alkaloids; Amaryllidaceae; Anti-inflammatory; Cytotoxicity; Lycoris aurea