title: Expanding P450 catalytic reaction space through evolution and engineering.
abstract: Advances in protein and metabolic engineering have led to wider use of enzymes to synthesize important molecules. However, many desirable transformations are not catalyzed by any known enzyme, driving interest in understanding how new enzymes can be created. The cytochrome P450 enzyme family, whose members participate in xenobiotic metabolism and natural products biosynthesis, catalyzes an impressive range of difficult chemical reactions that continues to grow as new enzymes are characterized. Recent work has revealed that P450-derived enzymes can also catalyze useful reactions previously accessible only to synthetic chemistry. The evolution and engineering of these enzymes provides an excellent case study for how to genetically encode new chemistry and expand biology's reaction space.
authors.affiliation: Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA, 91125, USA.
authors.forename: John A
authors.initials: JA
authors.lastname: McIntosh
authors.affiliation: Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA, 91125, USA.
authors.forename: Christopher C
authors.initials: CC
authors.lastname: Farwell
authors.affiliation: Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA, 91125, USA. Electronic address: frances@cheme.caltech.edu.
authors.forename: Frances H
authors.initials: FH
authors.lastname: Arnold
mesh_terms: D055162:Biocatalysis; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D010084:Oxidation-Reduction; D015202:Protein Engineering
chemical_list: D003577:Cytochrome P-450 Enzyme System