title: Essential oil composition of Senecio graciliflorus DC: comparative analysis of different parts and evaluation of antioxidant and cytotoxic activities.
abstract: The essential oil of different parts of Senecio graciliflorus DC was obtained by hydrodistillation and analysed by GC-FID and GC-MS for the first time. A total of 17, 20, 19 and 17 constituents were identified comprising 99.90, 95.50, 98.93 and 95.96% of the essential oil of flower, leaf, stem and root parts of Senecio graciliflorus respectively. Monoterpene hydrocarbons predominated in the essential oil with 85.28% in flower, 57.53% in leaf, 67.74% in stem and 64.98% in root oil. α-pinene, cis-ocimene, 1,2,3-trimethylcyclohexane and β-pinene were the major constituents of the essential oil. The flower essential oil exhibited a strong antioxidant potential displaying IC50 values of 21.6±0.6 and 26.0±1.0μg/ml in DPPH and hydroxyl radical assays respectively. On the other hand the essential oil of flower and root displayed highest cytotoxicity against lung (A-549) cancer cell lines (IC50=19.1±0.9 and 21.3±1.1μg/ml respectively. This study which represents the first report of the essential oil composition and bioevaluation of Senecio graciliflorus, can serve as a new source of cytotoxic and antioxidant activity.
authors.affiliation: Bioorganic Chemistry Division, Indian Institute of Integrative Medicine (CSIR), Srinagar 190005, Jammu and Kashmir, India.
authors.forename: Shabir H
authors.initials: SH
authors.lastname: Lone
authors.affiliation: Bioorganic Chemistry Division, Indian Institute of Integrative Medicine (CSIR), Srinagar 190005, Jammu and Kashmir, India. Electronic address: kabhat@iiim.ac.in.
authors.forename: Khursheed A
authors.initials: KA
authors.lastname: Bhat
authors.affiliation: Bioorganic Chemistry Division, Indian Institute of Integrative Medicine (CSIR), Srinagar 190005, Jammu and Kashmir, India.
authors.forename: Haroon M
authors.initials: HM
authors.lastname: Bhat
authors.affiliation: Cancer Pharmacology Division, Indian Institute of Integrative Medicine (CSIR), Jammu 180001, Jammu and Kashmir, India.
authors.forename: Rabiya
authors.initials: R
authors.lastname: Majeed
authors.affiliation: Instrumentation Division, Indian Institute of Integrative Medicine (CSIR), Jammu 180001, Jammu and Kashmir, India.
authors.forename: Rajneesh
authors.initials: R
authors.lastname: Anand
authors.affiliation: Cancer Pharmacology Division, Indian Institute of Integrative Medicine (CSIR), Jammu 180001, Jammu and Kashmir, India.
authors.forename: Abid
authors.initials: A
authors.lastname: Hamid
authors.affiliation: Department of Chemistry, University of Kashmir, Srinagar 190006, India.
authors.forename: Mohd A
authors.initials: MA
authors.lastname: Khuroo
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000032:analysis / Q000494:pharmacology / Q000627:therapeutic use*; D000975:Antioxidants / Q000032:analysis / Q000494:pharmacology / Q000627:therapeutic use*; D000081008:Bicyclic Monoterpenes; D001643:Bridged Bicyclo Compounds / Q000032:analysis; D045744:Cell Line, Tumor; D003510:Cyclohexanes / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D020128:Inhibitory Concentration 50; D008175:Lung Neoplasms / Q000188:drug therapy*; D039821:Monoterpenes / Q000032:analysis; D009822:Oils, Volatile / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D010938:Plant Oils / Q000737:chemistry; D018514:Plant Structures / Q000737:chemistry*; D012674:Senecio / Q000737:chemistry*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D000081008:Bicyclic Monoterpenes; D001643:Bridged Bicyclo Compounds; D003510:Cyclohexanes; D039821:Monoterpenes; D009822:Oils, Volatile; D010936:Plant Extracts; D010938:Plant Oils; C010789:beta-pinene; C005451:alpha-pinene
keywords: Antioxidant; Cytotoxicity; Essential oil; Senecio graciliflorus DC