title: Transplanted neural stem cells modulate regulatory T, γδ T cells and corresponding cytokines after intracerebral hemorrhage in rats.
abstract: The immune system, particularly T lymphocytes and cytokines, has been implicated in the progression of brain injury after intracerebral hemorrhage (ICH). Although studies have shown that transplanted neural stem cells (NSCs) protect the central nervous system (CNS) from inflammatory damage, their effects on subpopulations of T lymphocytes and their corresponding cytokines are largely unexplored. Here, rats were subjected to ICH and NSCs were intracerebrally injected at 3 h after ICH. The profiles of subpopulations of T cells in the brain and peripheral blood were analyzed by flow cytometry. We found that regulatory T (Treg) cells in the brain and peripheral blood were increased, but γδT cells (gamma delta T cells) were decreased, along with increased anti-inflammatory cytokines (IL-4, IL-10 and TGF-β) and decreased pro-inflammatory cytokines (IL-6, and IFN-γ), compared to the vehicle-treated control. Our data suggest that transplanted NSCs protect brain injury after ICH via modulation of Treg and γδT cell infiltration and anti- and pro-inflammatory cytokine release.
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital, Wenzhou Medical University, Wenzhou 325000, Zhejiang, China. gl2008123@126.com.
authors.forename: Lu
authors.initials: L
authors.lastname: Gao
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital, Wenzhou Medical University, Wenzhou 325000, Zhejiang, China. happycatlq1001@gmail.com.
authors.forename: Qin
authors.initials: Q
authors.lastname: Lu
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital, Wenzhou Medical University, Wenzhou 325000, Zhejiang, China. lijiehuangwy@gmail.com.
authors.forename: Li-Jie
authors.initials: LJ
authors.lastname: Huang
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital, Wenzhou Medical University, Wenzhou 325000, Zhejiang, China. rlh1232@hotmail.com.
authors.forename: Lin-Hui
authors.initials: LH
authors.lastname: Ruan
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital, Wenzhou Medical University, Wenzhou 325000, Zhejiang, China. jianjingyang11@hotmail.com.
authors.forename: Jian-Jing
authors.initials: JJ
authors.lastname: Yang
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital, Wenzhou Medical University, Wenzhou 325000, Zhejiang, China. weilongh84@hotmail.com.
authors.forename: Wei-Long
authors.initials: WL
authors.lastname: Huang
authors.affiliation: Zhejiang Provincial Key Laboratory of Aging and Neurological Disorder Research, First Afﬁliated Hospital, Wenzhou Medical University, Wenzhou 325000, Zhejiang, China. cupidjiro@gmail.com.
authors.forename: Wei-Shan
authors.initials: WS
authors.lastname: ZhuGe
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital, Wenzhou Medical University, Wenzhou 325000, Zhejiang, China. drzhangyl33@gmail.com.
authors.forename: Yong-Liang
authors.initials: YL
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital, Wenzhou Medical University, Wenzhou 325000, Zhejiang, China. drxf39@gmail.com.
authors.forename: Biao
authors.initials: B
authors.lastname: Fu
authors.affiliation: Zhejiang Provincial Key Laboratory of Aging and Neurological Disorder Research, First Afﬁliated Hospital, Wenzhou Medical University, Wenzhou 325000, Zhejiang, China. Kunlin.Jin@unthsc.edu.
authors.forename: Kun-Lin
authors.initials: KL
authors.lastname: Jin
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital, Wenzhou Medical University, Wenzhou 325000, Zhejiang, China. gl2008123@126.com.
authors.forename: Qi-Chuan
authors.initials: QC
authors.lastname: ZhuGe
mesh_terms: D000818:Animals; D001921:Brain / Q000098:blood supply / Q000276:immunology / Q000473:pathology; D002478:Cells, Cultured; D002543:Cerebral Hemorrhage / Q000097:blood / Q000276:immunology* / Q000628:therapy; D016207:Cytokines / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D007371:Interferon-gamma / Q000097:blood / Q000276:immunology; D016753:Interleukin-10 / Q000097:blood / Q000276:immunology; D015847:Interleukin-4 / Q000097:blood / Q000276:immunology; D015850:Interleukin-6 / Q000097:blood / Q000276:immunology; D008297:Male; D008810:Mice, Inbred C57BL; D058953:Neural Stem Cells / Q000276:immunology* / Q000637:transplantation; D017207:Rats, Sprague-Dawley; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology / Q000378:metabolism; D033581:Stem Cell Transplantation / Q000379:methods; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D016212:Transforming Growth Factor beta / Q000097:blood / Q000276:immunology; D014183:Transplantation, Heterologous
chemical_list: D016207:Cytokines; D015850:Interleukin-6; D016692:Receptors, Antigen, T-Cell, gamma-delta; D016212:Transforming Growth Factor beta; D016753:Interleukin-10; D015847:Interleukin-4; D007371:Interferon-gamma