title: MicroRNA-432 contributes to dopamine cocktail and retinoic acid induced differentiation of human neuroblastoma cells by targeting NESTIN and RCOR1 genes.
abstract: MicroRNA (miRNA) regulates expression of protein coding genes and has been implicated in diverse cellular processes including neuronal differentiation, cell growth and death. To identify the role of miRNA in neuronal differentiation, SH-SY5Y and IMR-32 cells were treated with dopamine cocktail and retinoic acid to induce differentiation. Detection of miRNAs in differentiated cells revealed that expression of many miRNAs was altered significantly. Among the altered miRNAs, human brain expressed miR-432 induced neurite projections, arrested cells in G0-G1, reduced cell proliferation and could significantly repress NESTIN/NES, RCOR1/COREST and MECP2. Our results reveal that miR-432 regulate neuronal differentiation of human neuroblastoma cells.
authors.affiliation: Crystallography and Molecular Biology Division, Saha Institute of Nuclear Physics, 1/AF Bidhan Nagar, Kolkata 700064, India.
authors.forename: Eashita
authors.initials: E
authors.lastname: Das
authors.affiliation: Crystallography and Molecular Biology Division, Saha Institute of Nuclear Physics, 1/AF Bidhan Nagar, Kolkata 700064, India. Electronic address: nitaipada.bhattacharya@saha.ac.in.
authors.forename: Nitai Pada
authors.initials: NP
authors.lastname: Bhattacharyya
mesh_terms: D020413:3' Untranslated Regions; D001483:Base Sequence; D001665:Binding Sites; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D056970:Co-Repressor Proteins; D004298:Dopamine / Q000494:pharmacology / Q000502:physiology*; D059585:G1 Phase Cell Cycle Checkpoints; D006801:Humans; D051783:Methyl-CpG-Binding Protein 2 / Q000235:genetics / Q000378:metabolism; D035683:MicroRNAs / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000378:metabolism; D064231:Nestin / Q000235:genetics* / Q000378:metabolism; D016501:Neurites / Q000378:metabolism; D034622:RNA Interference; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism; D014212:Tretinoin / Q000494:pharmacology / Q000502:physiology*
chemical_list: D020413:3' Untranslated Regions; D015415:Biomarkers; D056970:Co-Repressor Proteins; C497392:MECP2 protein, human; C582553:MIRN432 microRNA, human; D051783:Methyl-CpG-Binding Protein 2; D035683:MicroRNAs; C577484:NES protein, human; D009419:Nerve Tissue Proteins; D064231:Nestin; C121019:RCOR1 protein, human; D012097:Repressor Proteins; D014212:Tretinoin; D004298:Dopamine
keywords: MECP2; MicroRNA-432; NESTIN; Neurite outgrowth; RCOR1; SH-SY5Y cells