title: [Determination of urinary 8-hydroxy-2'-deoxyguanosine, trans, trans-muconic acid, and S-phenylmercapturic acid by liquid chromatography-mass spectrometry].
abstract: OBJECTIVE
  To establish a method for simultaneously determining the urinary concentrations of 8-hydroxy-2'-deoxyguanosine (8-OHdG), trans, trans-muconic acid (tt-MA), and S-phenylmercapturic acid (S-PMA) in subjects exposed to benzene.
  
  
  METHODS
  After being purified by a solid-phase extraction column, the urine samples were transferred to a liquid chromatography-mass spectrometry system, and the concentrations of 8-OHdG, tt-MA, and S-PMA were determined by external standard method. A C18 reversed-phase column was used as the chromatographic column, and methanol/acidic ammonium formate solution was used as the mobile phase for gradient elution. The mass spectrometer was operated in a multi-reaction monitoring mode.
  
  
  RESULTS
  For tt-MA, the calibration curves were linear in the range of 10-1000 µg/L, and the recovery rates were over 90% (relative standard deviation (RSD) < 3%) at spiked levels of 50 µg/L and 500 µg/L. For S-PMA and 8-OHdG, the calibration curves were linear in the range of 1-100 µg/L, and the recovery rates were over 85% (RSD < 5%) at spiked levels of 5 µg/L and 50 µg/L.
  
  
  CONCLUSIONS
  This determination method meets the requirement of Biological materials-
  
  
  METHODS
  of monitoring-Guide of development (WS/T 68-1996) and can be used for simultaneous determination of 8-OHdG, tt-MA, and S-PMA in urine.
authors.affiliation: Jiangsu Provincial Center for Disease Control and Prevention, Nanjing 210009, China.
authors.forename: Hualiang
authors.initials: H
authors.lastname: Liu
authors.affiliation: Jiangsu Provincial Center for Disease Control and Prevention, Nanjing 210009, China. E-mail: zhubl@jscdc.cn.
authors.forename: Baoli
authors.initials: B
authors.lastname: Zhu
authors.affiliation: Jiangsu Provincial Center for Disease Control and Prevention, Nanjing 210009, China.
authors.forename: Junwen
authors.initials: J
authors.lastname: Shi
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000111:Acetylcysteine / Q000031:analogs & derivatives* / Q000652:urine; D001554:Benzene / Q000506:poisoning*; D002853:Chromatography, Liquid / Q000379:methods; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000652:urine; D006801:Humans; D013058:Mass Spectrometry; D016273:Occupational Exposure / Q000517:prevention & control*; D013011:Sorbic Acid / Q000031:analogs & derivatives* / Q000378:metabolism
chemical_list: C005939:muconic acid; C053003:S-phenyl-N-acetylcysteine; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine; D001554:Benzene; D000111:Acetylcysteine; D013011:Sorbic Acid