title: Altered pattern of Naïve and memory B cells and B1 cells in patients with chronic granulomatous disease.
abstract: Chronic granulomatous disease (CGD) is a rare primary immunodeficiency disorder characterized by a greatly increased susceptibility to severe fungal and bacterial infections caused by defects in NADPH oxidase of phagocytic cells. We aimed to investigate immunophenotype alterations of naïve and memory B cells and B1a cells in peripheral whole blood from Iranian patients with CGD. Flow cytometric analysis was performed on peripheral blood samples from 31 CGD patients and 23 healthy controls (HC) to study naïve (IgD+/CD27-), memory (CD27+) B and B1a (CD5+) cells. Soluble CD27 (sCD27) and immunoglobulins were also measured by ELISA and the nephelometric method, respectively. We found significantly higher levels of naïve B cells and B1a cells but lower levels of memory B cells in CGD patients compared to HC.. There was no significant difference in soluble CD27 (sCD27) alteration between CGD patients and HC. Our findings suggested a role for NADPH oxidase in process of B cell differentiation and impairing conversion of naïve B cells to memory B cells and altered B1a cells in CGD patients. Increased susceptibility of CGD patients to opportunistic infections and autoimmune disorders could be partly explained by the altered phenotype of B lymphocytes in these patients.
authors.affiliation: Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. m-mohsenzadegan@razi.tums.ac.ir.
authors.forename: Monireh
authors.initials: M
authors.lastname: Mohsenzadegan
authors.affiliation: Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran. pourpakz@tums.ac.ir.
authors.forename: Fahimeh
authors.initials: F
authors.lastname: Fattahi
authors.affiliation: Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran and Department of Pathology and Medical Biology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands. ffattahi@outlook.com.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Fattahi
authors.affiliation: Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. mirshafiey@tums.ac.ir.
authors.forename: Abbas
authors.initials: A
authors.lastname: Mirshafiey
authors.affiliation: Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran. fazlollahi@tums.ac.ir.
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Fazlollahi
authors.affiliation: Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. aisan5952@gmail.com.
authors.forename: Fariba
authors.initials: F
authors.lastname: Naderi Beni
authors.affiliation: Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran and Department of Immunology and Allergy, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran. Movahedm@tums.ac.ir.
authors.forename: Masoud
authors.initials: M
authors.lastname: Movahedi
authors.affiliation: Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran and Department of Immunology and Allergy, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran. pourpakz@tums.ac.ir.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Pourpak
mesh_terms: D000293:Adolescent; D000328:Adult; D016175:B-Lymphocyte Subsets / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D005260:Female; D005434:Flow Cytometry; D006105:Granulomatous Disease, Chronic / Q000276:immunology*; D006801:Humans; D007136:Immunoglobulins / Q000097:blood; D007156:Immunologic Memory; D008297:Male; D019255:NADPH Oxidases / Q000502:physiology; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7 / Q000032:analysis
chemical_list: D007136:Immunoglobulins; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7; D019255:NADPH Oxidases