title: Cancer stem cell-like characteristics of a CD133+subpopulation in the J82 human bladder cancer cell line.
abstract: Cancer stem cells (CSCs) are thought to be crucial for understanding the biological roots of cancer, and are of increasing importance as a target for new anticancer agents. According to an expression analysis of the cell surface antigens of various types of cancer, CD133 is considered to be a potential marker of cancer stemness. In this study, a human urinary bladder cancer cell line (J82) was used to analyze the cancer stem cell-like characteristics of CD133+bladder cancer cellsin vitroandin vivo. The CD133 expression in the J82 cells was examined and the cells were immunomagnetically categorized into positive and negative subsets. The CD133-and CD133+subsets were phenotypically divergent with regard to the cell growth pattern, while CD133+cells tended to colonize during their growth. In CD133+cells, the pluripotent stem cell factors Oct-4 and Sox-2 were upregulated, and a statistically significant proliferation increase was observed when compared to CD133-cells. The CD133+subpopulation was more tolerant to the chemotherapeutic agent cisplatin, and Bacillus Calmette-Guérin (BCG), an agent instilled intravesically to treat bladder cancer. In addition, CD133+J82 cells were more resistant to radiation treatment when compared to CD133-cells. Thein vivotumorigenesis of the CD133-and CD133+subsets of J82 cancer cells was also examined by subcutaneously injecting them into nude mice. The tumor growth was more aggressive in the CD133+subpopulation, showing a significant difference in the tumorigenic potential in these subsets. In conclusion, J82 human bladder cancer cells include CD133-and CD133+subpopulations, while the CD133 molecule is a potential marker of the potential malignancy of human bladder cancer. In the present study, the CD133+subpopulation was herein demonstrated to have certain characteristics consistent with those of cancer stem cells.
authors.affiliation: Center for Gene and Cell Therapy ; Department of Urology, Zhujiang Hospital, Southern Medical University, Guangzhou, P.R. China.
authors.forename: Peng
authors.initials: P
authors.lastname: Huang
authors.affiliation: Center for Gene and Cell Therapy.
authors.forename: Masami
authors.initials: M
authors.lastname: Watanabe
authors.affiliation: Center for Gene and Cell Therapy.
authors.forename: Haruki
authors.initials: H
authors.lastname: Kaku
authors.affiliation: Department of Urology.
authors.forename: Hideo
authors.initials: H
authors.lastname: Ueki
authors.affiliation: Gastroenterological Surgery and.
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Noguchi
authors.affiliation: Department of Urology.
authors.forename: Morito
authors.initials: M
authors.lastname: Sugimoto
authors.affiliation: Department of Urology.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Hirata
authors.affiliation: Neuroscience, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Japan;
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yamada
authors.affiliation: Neuroscience, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Japan;
authors.forename: Kohji
authors.initials: K
authors.lastname: Takei
authors.affiliation: Department of Urology, Zhujiang Hospital, Southern Medical University, Guangzhou, P.R. China.
authors.forename: Shaobo
authors.initials: S
authors.lastname: Zheng
authors.affiliation: Department of Urology, Zhujiang Hospital, Southern Medical University, Guangzhou, P.R. China.
authors.forename: Kai
authors.initials: K
authors.lastname: Xu
authors.affiliation: Center for Gene and Cell Therapy.
authors.forename: Yasutomo
authors.initials: Y
authors.lastname: Nasu
authors.affiliation: Innovation Center Okayama for Nanobio-Targeted Therapy and Departments of.
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Fujii
authors.affiliation: Department of Urology, Zhujiang Hospital, Southern Medical University, Guangzhou, P.R. China.
authors.forename: Chunxiao
authors.initials: C
authors.lastname: Liu
authors.affiliation: Center for Gene and Cell Therapy.
authors.forename: Hiromi
authors.initials: H
authors.lastname: Kumon
keywords: CD133; Oct-4; bladder cancer; cancer stem cells; drug resistance