title: Mesenchymal stem cells repair germinal cells of seminiferous tubules of sterile rats.
abstract: BACKGROUND
  Mesenchymal stem cells (MSCs) are undifferentiated cells that can differentiate and divide to other cell types. Transplantation of these cells to the different organs is used for curing various diseases.
  
  
  OBJECTIVE
  The aim of this research was whether MSCs transplantation could treat the sterile testes.
  
  
  METHODS
  In this experimental study, Donor MSCs were isolated from bone marrow of Wistar rats. The recipients were received 40 mg/kg of busulfan to stop endogenous spermatogenesis. The MSCs were injected into the left testes. Cell tracing was done by labeling the MSCs by 5-Bromo-2- Deoxy Uridine (BrdU). The immunohistochemical and morphometrical studies were performed to analysis the curing criteria.
  
  
  RESULTS
  The number of spermatogonia (25.38±1.57), primary spermatocytes (55.41±1.62) and spermatozoids (4.95±1.30)×10(6) in busulfan treated animals were decreased significantly as compared to the control group (33.35±1.78, 64.44±2.00) and (10.50±1.82)×10(6) respectively but stem cells therapy help the spermatogenesis begin more effective in these animals (32.78±1.99, 63.59±2.01) and (9.81±1.33)×10(6) respectively than the control group. The injected BrdU labeled mesenchymal stem cells differentiated to spermatogonia and spermatozoa in the seminiferous tubules of the infertile testis and also to the interstitial cells between tubules.
  
  
  CONCLUSIONS
  We concluded that testis of host infertile rats accepted transplanted MSCs. The transplanted MSCs could differentiate into germinal cells in testicular seminiferous tubules. This article extracted from M.Sc. Thesis. (Bentolhoda Fereydouni).
authors.affiliation: Department of Biology, College of Sciences, Shiraz University, Shiraz, Iran.
authors.forename: Malihezaman
authors.initials: M
authors.lastname: Monsefi
authors.affiliation: Department of Biology, College of Sciences, Shiraz University, Shiraz, Iran.
authors.forename: Bentolhoda
authors.initials: B
authors.lastname: Fereydouni
authors.affiliation: Department of Anatomy, Laboratory for Stem Cell Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Leili
authors.initials: L
authors.lastname: Rohani
authors.affiliation: Department of Anatomy, Laboratory for Stem Cell Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Tahereh
authors.initials: T
authors.lastname: Talaei
keywords: Cell therapy; Germinal cells; Immunohistochemistry; Mesenchymal stem cells