title: The genomic RNA1 and RNA2 sequences of the tobacco rattle virus isolates found in Polish potato fields.
abstract: Four tobacco rattle virus (TRV) isolates were identified from tobacco bait seedlings planted in soil samples from Polish potato fields. Sequence analysis of the genomic RNA1 of the isolates revealed significant similarity to the isolates Ho and AL recently found in Germany. Multiple sequence alignments of the genomic RNA2 indicated that the two isolates from northern Poland (Deb57 and Slu24) are in a cluster with the isolates PSG and PLB found in the Netherlands. The remaining two isolates, from central Poland (11r21 and Mlo7), are in a distinct group with the unique isolate SYM found in England. The RNA2 sequences of the studied isolates range from 1998 nt to 2739 nt in length, and all carry deletions of the 2b and/or 2c genes. The isolate Mlo7 has an atypical RNA2 structure, having its cp gene located in its central region.
authors.affiliation: Młochów Research Center, Plant Breeding and Acclimatization Institute - National Research Institute, Platanowa Street 19, PL-05-831 Młochów, Poland. Electronic address: z.yin@ihar.edu.pl.
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Yin
authors.affiliation: Department of Plant Genetics, Breeding & Biotechnology, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences - SGGW, Nowoursynowska Street 159, PL-02-776 Warsaw, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Pawełkowicz
authors.affiliation: Młochów Research Center, Plant Breeding and Acclimatization Institute - National Research Institute, Platanowa Street 19, PL-05-831 Młochów, Poland.
authors.forename: Krystyna
authors.initials: K
authors.lastname: Michalak
authors.affiliation: Młochów Research Center, Plant Breeding and Acclimatization Institute - National Research Institute, Platanowa Street 19, PL-05-831 Młochów, Poland.
authors.forename: Mirosława
authors.initials: M
authors.lastname: Chrzanowska
authors.affiliation: Młochów Research Center, Plant Breeding and Acclimatization Institute - National Research Institute, Platanowa Street 19, PL-05-831 Młochów, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Zimnoch-Guzowska
mesh_terms: D016679:Genome, Viral; D010935:Plant Diseases / Q000821:virology*; D010942:Plant Viruses / Q000235:genetics* / Q000302:isolation & purification; D011044:Poland; D012367:RNA, Viral / Q000235:genetics*; D011198:Solanum tuberosum / Q000821:virology*
chemical_list: D012367:RNA, Viral
keywords: Genome sequence; Poland; Potato; RNA1; RNA2; Recombination; Tobacco rattle virus