title: [Evaluation of the commercial ELISA test-systems of different formats to detect specific IgM and IgG in the measles patients sera].
abstract: Nine commercial kits of "captured" and "indirect" format ELISA assay for the detection of specific IgM and IgG in sera of patients with measles were compared to each other. 72 sera specimens from typical medium-severity cases from a measles outbreak (2010) were collected on the 5-6th day after the rash onset. IgM was detected with "capture" tests (Vecto-Measles IgM, Vector Best, Measles IgM capture EIA, Microimmune Ltd) close to 100% of cases, irrespectively to the age and the initial vaccination status of the patients. The IgM result was negative in 23.6% by average while investigating using "indirect" format tests (Enzygnost Anti-Measles Virusll/IgM, Siemens; Anti-Measles Viruses ELISA (IgM), Eurominimum, Virion-Serion IgM (GmbH). These cases were in adults, the majority of which had 1-2 vaccinations in the past. The analysis of the presented data shows high correlation connection between the tests used and high confidence level for OD IgM and IgG of the sera of the patients with the primary and secondary immune response.
authors.forename: T A
authors.initials: TA
authors.lastname: Mamaeva
authors.forename: M A
authors.initials: MA
authors.lastname: Naumova
authors.forename: N V
authors.initials: NV
authors.lastname: Zheleznova
authors.forename: G Iu
authors.initials: GIu
authors.lastname: Lipskaia
authors.forename: M
authors.initials: M
authors.lastname: Mulders
authors.forename: D A
authors.initials: DA
authors.lastname: Featherstone
mesh_terms: D000328:Adult; D000888:Antibodies, Anti-Idiotypic / Q000737:chemistry / Q000276:immunology; D000914:Antibodies, Viral / Q000097:blood*; D000915:Antibody Affinity; D000918:Antibody Specificity; D002648:Child; D004196:Disease Outbreaks; D004797:Enzyme-Linked Immunosorbent Assay / Q000706:statistics & numerical data; D005260:Female; D006801:Humans; D007114:Immunization; D007074:Immunoglobulin G / Q000097:blood*; D007075:Immunoglobulin M / Q000097:blood*; D007223:Infant; D008297:Male; D008457:Measles / Q000097:blood* / Q000175:diagnosis / Q000453:epidemiology* / Q000276:immunology; D008459:Measles virus / Q000276:immunology; D011933:Reagent Kits, Diagnostic / Q000706:statistics & numerical data; D012426:Russia / Q000453:epidemiology; D012720:Severity of Illness Index
chemical_list: D000888:Antibodies, Anti-Idiotypic; D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011933:Reagent Kits, Diagnostic; C048844:anti-IgG; C044203:anti-IgM