title: Production of 1,3-propanediol from glycerol using the newly isolated Klebsiella pneumoniae J2B.
abstract: Recently, novel Klebsiella pneumoniae J2B, which grows rapidly on glycerol as the carbon source without forming pathogenic and sticky lipopolysaccharides, was isolated. Current study examined the ability of K. pneumoniae J2B to produce 1,3-propanediol (PDO) from glycerol. To this end, a deletion mutant for lactate formation was constructed. The ldhA mutant strain produced negligible lactate but more 2,3-butanediol (BDO). When K. pneumoniae ΔldhA was cultivated in glycerol fed-batch mode, the PDO titer of 58.0 g/L with a yield of 0.35 g/g and an overall volumetric productivity of 1.3g/L/h were obtained. BDO was the main byproduct (26.6g/L). Less than 10 g/L of the other metabolites was produced. As PDO and other metabolites accumulated, the rate of PDO production decreased significantly due mainly to the toxic effects of these metabolites. This study highlights the potential of newly isolated K. pneumoniae J2B for the production of PDO from glycerol.
authors.affiliation: School of Chemical and Biomolecular Engineering, Pusan National University, San 30, Jangjeon-dong, Geumjeong-gu, Busan 609-735, Republic of Korea.
authors.forename: Meetu
authors.initials: M
authors.lastname: Durgapal
authors.affiliation: School of Chemical and Biomolecular Engineering, Pusan National University, San 30, Jangjeon-dong, Geumjeong-gu, Busan 609-735, Republic of Korea.
authors.forename: Vinod
authors.initials: V
authors.lastname: Kumar
authors.affiliation: R&D Center, GS Caltex Corporation, 104-4 Munji-dong, Yusung-gu, Daejeon 305-380, Republic of Korea.
authors.forename: Taek Ho
authors.initials: TH
authors.lastname: Yang
authors.affiliation: R&D Center, GS Caltex Corporation, 104-4 Munji-dong, Yusung-gu, Daejeon 305-380, Republic of Korea.
authors.forename: Hee Jong
authors.initials: HJ
authors.lastname: Lee
authors.affiliation: R&D Center, GS Caltex Corporation, 104-4 Munji-dong, Yusung-gu, Daejeon 305-380, Republic of Korea.
authors.forename: Doyoung
authors.initials: D
authors.lastname: Seung
authors.affiliation: School of Chemical and Biomolecular Engineering, Pusan National University, San 30, Jangjeon-dong, Geumjeong-gu, Busan 609-735, Republic of Korea. Electronic address: parksh@pusan.ac.kr.
authors.forename: Sunghoon
authors.initials: S
authors.lastname: Park
mesh_terms: D000332:Aerobiosis / Q000187:drug effects; D061249:Batch Cell Culture Techniques; D019149:Bioreactors / Q000382:microbiology; D002244:Carbon / Q000378:metabolism; D003470:Culture Media / Q000494:pharmacology; D005285:Fermentation / Q000187:drug effects; D005990:Glycerol / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007711:Klebsiella pneumoniae / Q000166:cytology / Q000201:enzymology / Q000302:isolation & purification* / Q000378:metabolism*; D010084:Oxidation-Reduction / Q000187:drug effects; D011409:Propylene Glycols / Q000378:metabolism*; D012995:Solubility
chemical_list: D003470:Culture Media; D011409:Propylene Glycols; C041787:1,3-propanediol; D002244:Carbon; D005990:Glycerol
keywords: 1,3-Propanediol; 2,3-Butanediol; Glycerol; Klebsiella pneumoniae J2B; Lactate