title: Electrochemical sensor for ultrasensitive determination of isoquercitrin and baicalin based on DM-β-cyclodextrin functionalized graphene nanosheets.
abstract: In this study, 2,6-dimethyl-β-cyclodextrin (DM-β-CD) functionalized graphene nanosheets (DM-β-CD-GNs) were successfully synthesized by a simple wet-chemical strategy. The as obtained DM-β-CD-GNs were characterized by UV-vis spectroscopy, Fourier transform Infrared (FT-IR) spectroscopy, atomic force microscopy (AFM) and transmission electron microscopy (TEM). The new nanocomposite possesses the unique properties of graphene (large surface area and high conductivity) and DM-β-CD (high supramolecular recognition and enrichment capability). Based on the above properties, a highly sensitive electrochemical sensor was developed to detect two flavonoid drugs (isoquercitrin and baicalin). At the DM-β-CD-GNs modified glassy carbon electrode (DM-β-CD-GNs/GCE), the peak currents of the two drugs increased dramatically compared with that on the bare GCE and GNs/GCE which due to the synergetic effects of GNs and DM-β-CD molecules. The linear response ranges for isoquercitrin and baicalin are 10nM-3.0μM and 0.04μM -3.0μM, with the detection limits of 4nM and 10nM, respectively. The method might open up a new possibility for the widespread use of electrochemical sensors for monitoring of ultra-trace flavonoid drugs owing to its advantages of simple preparation, low cost, high sensitivity, good stability and reproducibility.
authors.affiliation: Institute of Environmental Science, Shanxi University, Taiyuan 030006, China.
authors.forename: Zhiguang
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Institute of Environmental Science, Shanxi University, Taiyuan 030006, China.
authors.forename: Ai
authors.initials: A
authors.lastname: Zhang
authors.affiliation: Institute of Environmental Science, Shanxi University, Taiyuan 030006, China. Electronic address: guoyj@sxu.edu.cn.
authors.forename: Yujing
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Institute of Environmental Science, Shanxi University, Taiyuan 030006, China. Electronic address: dc@sxu.edu.cn.
authors.forename: Chuan
authors.initials: C
authors.lastname: Dong
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D003217:Conductometry / Q000295:instrumentation*; D004566:Electrodes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005419:Flavonoids / Q000032:analysis* / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D011794:Quercetin / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry; D013499:Surface Properties; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D005419:Flavonoids; D047392:beta-Cyclodextrins; C016527:isoquercitrin; C038044:baicalin; C038119:heptakis(2,6-O-dimethyl)beta-cyclodextrin; D006108:Graphite; D011794:Quercetin
keywords: 2,6-Dimethyl-β-cyclodextrin; Baicalin; Electrochemical sensor; Graphene; Isoquercitrin