title: GDNF fails to inhibit LPS-mediated activation of mouse microglia.
abstract: GDNF is a potent neuroprotective factor for midbrain dopaminergic (mDA) neurons. In LPS-mediated models for mDA degeneration GDNF increases neuron survival and further reduces microglia activation. To elucidate the effects of GDNF on LPS-induced activation, primary microglia from C57BL/6 and NMRI mice have been analysed. In this study we demonstrate that GDNF is not able to inhibit LPS-mediated upregulation and release of the proinflammatory factors IL6 and TNFα. Moreover, we provide evidence that mouse microglia, in contrast to rat microglia, lack expression of the GDNF signalling receptor c-Ret resulting in abrogated activation of downstream signalling kinases Akt and Erk1/2.
authors.affiliation: Institute for Anatomy and Cell Biology, Department of Molecular Embryology, Albert-Ludwigs-University Freiburg, Germany.
authors.forename: Anke
authors.initials: A
authors.lastname: Zlotnik
authors.affiliation: Institute for Anatomy and Cell Biology, Department of Molecular Embryology, Albert-Ludwigs-University Freiburg, Germany. Electronic address: bjoern.spittau@anat.uni-freiburg.de.
authors.forename: Björn
authors.initials: B
authors.lastname: Spittau
mesh_terms: D000818:Animals; D016207:Cytokines / Q000096:biosynthesis; D004797:Enzyme-Linked Immunosorbent Assay; D051100:Glial Cell Line-Derived Neurotrophic Factor / Q000276:immunology* / Q000378:metabolism*; D015151:Immunoblotting; D007150:Immunohistochemistry; D008070:Lipopolysaccharides / Q000633:toxicity; D051379:Mice; D008810:Mice, Inbred C57BL; D017628:Microglia / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism*; D009410:Nerve Degeneration / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000276:immunology*
chemical_list: D016207:Cytokines; D051100:Glial Cell Line-Derived Neurotrophic Factor; D008070:Lipopolysaccharides
keywords: GDNF; Gfrα1; LPS; Microglia; c-Ret