title: Synergistic antibacterial effect of honey and Herba Ocimi Basilici against some bacterial pathogens.
abstract: OBJECTIVE
  To evaluate the antibacterial activity of the combination of different honey brands and methanolic fraction of Herba Ocimi Basilici using agar well diffusion assay.
  
  
  METHODS
  The antibacterial activity was determined against thirteen pathogenic bacterial clinical isolates including six gram negative (Klebsiella pneumonia, Pseudomonas aeroginosa, Escherichia coli, Salmonella typhi, Salmonella typhimirium, Xanthomonas campestris) and six gram positive strains (Enterococcus faecalis faecalis, Bacillus subtilis, Staphylococcus aureus, Clostridium perfringens type C, Clostridium perfringens type D, Clostridium chauvoei). Agar well diffusion method was used while zones of inhibition were measured with vernier scale.
  
  
  RESULTS
  At higher concentration, all the honey brands showed good to significant activity. The highest activity was observed for Hamdard brand honey (27.60 +/- 0.40) against Enterococcus faecalis.
  
  
  CONCLUSIONS
  These results revealed that combinations of plant extracts of Herba Ocimi Basilici with honey can be used for the development of potent and novel antibacterial agents.
authors.forename: Ali Talha
authors.initials: AT
authors.lastname: Khalil
authors.forename: Imran
authors.initials: I
authors.lastname: Khan
authors.forename: Kafeel
authors.initials: K
authors.lastname: Ahmad
authors.forename: Yusra Ali
authors.initials: YA
authors.lastname: Khan
authors.forename: Momin
authors.initials: M
authors.lastname: Khan
authors.forename: Muhammad Jaseem
authors.initials: MJ
authors.lastname: Khan
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects*; D004357:Drug Synergism; D006722:Honey / Q000032:analysis*; D008826:Microbial Sensitivity Tests; D018649:Ocimum basilicum / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts