title: Effect of photodynamic therapy with hypocrellin B on apoptosis, adhesion, and migration of cancer cells.
abstract: OBJECTIVE
  In the present study, we investigated effects of photodynamic therapy with hypocrellin B on apoptosis, adhesion, and migration of cancer cells in vitro.
  
  
  METHODS
  Human ovarian cancer HO-8910 cell as a cancer model cell was incubated with hypocrellin B at a concentration of 2.5 μM for 5 h and irradiated by light from a light-emitting diodes (LED) source. Cell apoptosis was analyzed by flow cytometry with annexin V/propidium iodide (PI) staining and nuclear staining 6 h after hypocrellin B photoirradiation. Cell adhesion was assessed using the 3-(4, 5-dimthylthiazol-2-yl)-2, 5 diphenyl-tetrazolium bromide (MTT) assay 4 h after photodynamic treatment. Cell migration was measured 48 h after photodynamic treatment.
  
  
  RESULTS
  Flow cytometry with annexin V/PI staining showed that early apoptotic and late apoptotic (necrotic) rates following photodynamic therapy with hypocrellin B markedly increased to 16.40% and 24.67%, respectively. Nuclear staining found nuclear condensation and typical apoptotic body in the treated cells. The number of cell migration was significantly decreased to 183 ± 28 after photodynamic therapy with hypocrellin B (p < 0.01). Light irradiation alone and hypocrellin B alone had no significant effect on cell migration. The cell adhesion inhibitory rate due to photodynamic action of hypocrellin B was 53.2 ± 1.8%, significantly higher than 2.7 ± 2.1% of light treatment alone and 1.0 ± 0.4% of hypocrellin B treatment alone (p < 0.01).
  
  
  CONCLUSIONS
  The findings demonstrated that photodynamic therapy with hypocrellin B remarkably induced apoptosis and inhibited adhesion and migration of cancer cells in vitro.
authors.affiliation: Department of Rehabilitation Medicine, First Affiliated Hospital of Chengdu Medical College , Sichuan.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Jiang
authors.forename: Albert Wingnang
authors.initials: AW
authors.lastname: Leung
authors.forename: Xinna
authors.initials: X
authors.lastname: Wang
authors.forename: Hongwei
authors.initials: H
authors.lastname: Zhang
authors.forename: Chuanshan
authors.initials: C
authors.lastname: Xu
mesh_terms: D017209:Apoptosis / Q000187:drug effects* / Q000528:radiation effects*; D002448:Cell Adhesion / Q000187:drug effects / Q000528:radiation effects; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects* / Q000528:radiation effects*; D002467:Cell Nucleus / Q000187:drug effects / Q000528:radiation effects; D005260:Female; D006801:Humans; D010051:Ovarian Neoplasms / Q000473:pathology*; D010569:Perylene / Q000031:analogs & derivatives* / Q000494:pharmacology; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000494:pharmacology; D011809:Quinones / Q000494:pharmacology*
chemical_list: D017319:Photosensitizing Agents; D011809:Quinones; D010569:Perylene; C064522:hypocrellin B
keywords: Hypocrellin B; adhesion; apoptosis; migration; ovarian cancer; photodynamic therapy