title: Roseomonas aceris sp. nov. isolated from a mono maple tree in the Shirakami Mountains in Japan.
abstract: A novel bacterial strain belonging to the genus Roseomonas was isolated from the trunk surface of a mono maple (Acer mono) tree growing in the Shirakami Mountains. The strain, designated R-1(T), was Gram-negative, non-motile, and oval-rod, and formed reddish colonies on agar plates, as has previously been described for Roseomonas species. Although motility was not observed, cells were peritrichously flagellated. Strain R-1(T) preferred organic acids over carbohydrates as growth substrates. The major cellular fatty acid was C₁₈:₁ ω7c (48.79%). Ubiquinone-10 was the major respiratory quinone. Strain R-1(T) demonstrated the highest 16S rRNA gene sequence similarity with Roseomonas pecuniae N75(T) (96.9%). Phylogenetic analysis based on 16S rRNA gene sequences confirmed that strain R-1(T) was a member of the genus Roseomonas and formed a cluster with R. pecuniae N75(T). DNA-DNA hybridization between strain R-1(T) and R. pecuniae N75(T) yielded 21.7% relatedness. On the basis of its phenotypic, phylogenetic, and chemotaxonomic char-acteristics, strain R-1(T) represents a novel species within the genus Roseomonas, for which the name Roseomonas aceris sp. nov. has been proposed. The type strain is R-1(T) (NBRC 109410(T) = DSM 26554 (T)).
authors.affiliation: Faculty of Agriculture and Life Science, Hirosaki University.
authors.forename: Akio
authors.initials: A
authors.lastname: Tonouchi
authors.forename: Daisuke
authors.initials: D
authors.lastname: Tazawa
mesh_terms: D031002:Acer / Q000382:microbiology*; D001483:Base Sequence; D005227:Fatty Acids / Q000378:metabolism; D005798:Genes, Bacterial; D020459:Genes, rRNA; D007564:Japan; D042082:Methylobacteriaceae / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D005227:Fatty Acids; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S