title: Origanum species native to the island of Crete: in vitro antioxidant characteristics and liquid chromatography-mass spectrometry identification of major polyphenolic components.
abstract: Extracts from three Origanum species, including Origanum microphyllum, Origanum dictamnus and Origanum vulgare subsp. hirtum, native to the island of Crete (southern Greece), were partly fractionated through successive partition with ethyl acetate and n-butanol. All the fractions obtained were profiled with regard to their major polyphenolic constituents, using liquid chromatography-diode array-mass spectrometry. Furthermore, the antioxidant potency of each fraction was assessed by estimating the antiradical activity (A(AR)) and the hydroxyl free radical scavenging activity (SA(HFR)). The chromatographic analyses revealed a rich profile mainly for the ethyl acetate fractions, composed principally by flavones, which were accompanied by a limited number of phenylpropanoids, flavanones and dihydroflavonols. The highest values of antioxidant activity were displayed by the ethyl acetate extract of O. dictamnus, which also possessed the richest polyphenolic composition.
authors.affiliation: a Food Quality & Chemistry of Natural Products, Mediterranean Agronomic Institute of Chania (M.A.I.Ch.)/Centre International de Hautes Etudes Agronomiques Méditerranéennen , P.O. Box 85, Chania 73100 , Greece.
authors.forename: Asma
authors.initials: A
authors.lastname: Tair
authors.forename: Erika-Krisztina
authors.initials: EK
authors.lastname: Weiss
authors.forename: Laurentiu Mihai
authors.initials: LM
authors.lastname: Palade
authors.forename: Sofia
authors.initials: S
authors.lastname: Loupassaki
authors.forename: Dimitris P
authors.initials: DP
authors.lastname: Makris
authors.forename: Efstathia
authors.initials: E
authors.lastname: Ioannou
authors.forename: Vassilios
authors.initials: V
authors.lastname: Roussis
authors.forename: Panagiotis
authors.initials: P
authors.lastname: Kefalas
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D002853:Chromatography, Liquid; D044950:Flavanones / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D006115:Greece; D015394:Molecular Structure; D031341:Origanum / Q000737:chemistry*; D010666:Phenylpropionates / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D059808:Polyphenols / Q000032:analysis* / Q000737:chemistry
chemical_list: D000975:Antioxidants; D044950:Flavanones; D005419:Flavonoids; D016166:Free Radical Scavengers; D010666:Phenylpropionates; D059808:Polyphenols
keywords: Origanum; antioxidants; flavonoids; liquid chromatography-diode array-mass spectrometry; polyphenols