title: Association of Toll-like receptor 2 polymorphisms with gout.
abstract: Gout is the most common autoinflammatory arthritis characterized by elevated serum urate and recurrent attacks of intra-articular crystal deposition of monosodium urate (MSU) in tissues. The pathogenesis of gout has not been fully determined, although certain genetic factors are involved in the development of gout. Accumulated data suggested that MSU crystal-induced inflammation is a paradigm of innate immunity. As Toll-like receptors (TLRs) are the underlying mechanisms of the innate immune response, the present study aimed to investigate whetherTLR2polymorphisms are associated with gout. Two single-nucleotide polymorphisms (Arg677Trp and Arg753Gln, rs5743708) inTLR2were genotyped by polymerase chain reaction-restriction fragment length polymorphism and the -196 to -174 del polymorphism was investigated using the allele-specific polymerase chain reaction in 431 individuals (215 patients with gout and 216 healthy controls).TLR2Arg677Trp and Arg753Gln genotyping indicated that all the positive samples were of the wild-type genotype. No significant differences in genotype (χ2=1.686, P=0.430) and allele (χ2=1.430, P=0.232) frequencies of the -196 to -174 del polymorphism between the patients with gout and the control groups was observed. Our results suggested that theTLR2Arg677Trp, Arg753Gln and the -196 to -174 del polymorphisms were not associated with susceptibility to primary gouty arthritis.
authors.affiliation: Faculty of Laboratory Medicine, North Sichuan Medical College, Nanchong, Sichuan 637000, P.R. China ; Department of Clinical Laboratory, The Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, P.R. China ; Institute of Rheumatism Immunity, The Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, P.R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Cai
authors.affiliation: Department of Endocrinology, The Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, P.R. China.
authors.forename: Yi-Hua
authors.initials: YH
authors.lastname: Peng
authors.affiliation: Faculty of Laboratory Medicine, North Sichuan Medical College, Nanchong, Sichuan 637000, P.R. China ; Department of Clinical Laboratory, The Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, P.R. China.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Tang
authors.affiliation: Faculty of Laboratory Medicine, North Sichuan Medical College, Nanchong, Sichuan 637000, P.R. China ; Department of Clinical Laboratory, The Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, P.R. China ; Institute of Rheumatism Immunity, The Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, P.R. China.
authors.forename: Xiao-Lan
authors.initials: XL
authors.lastname: Guo
authors.affiliation: Institute of Rheumatism Immunity, The Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, P.R. China ; Department of Immunology and Rheumatology, The Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, P.R. China.
authors.forename: Yu-Feng
authors.initials: YF
authors.lastname: Qing
authors.affiliation: Department of Biology, North Sichuan Medical College, Nanchong, Sichuan 637000, P.R. China.
authors.forename: Su-Hua
authors.initials: SH
authors.lastname: Liang
authors.affiliation: Institute of Rheumatism Immunity, The Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, P.R. China.
authors.forename: Hong
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Institute of Rheumatism Immunity, The Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, P.R. China ; Department of Immunology and Rheumatology, The Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, P.R. China.
authors.forename: Wang-Tai
authors.initials: WT
authors.lastname: Dang
authors.affiliation: Faculty of Laboratory Medicine, North Sichuan Medical College, Nanchong, Sichuan 637000, P.R. China ; Department of Clinical Laboratory, The Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, P.R. China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Ma
authors.affiliation: Faculty of Laboratory Medicine, North Sichuan Medical College, Nanchong, Sichuan 637000, P.R. China ; Department of Clinical Laboratory, The Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, P.R. China.
authors.forename: Cheng
authors.initials: C
authors.lastname: He
authors.affiliation: Institute of Rheumatism Immunity, The Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, P.R. China ; Department of Immunology and Rheumatology, The Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, P.R. China.
authors.forename: Jing-Guo
authors.initials: JG
authors.lastname: Zhou
keywords: Toll-like receptor 2; arthritis; gout; polymorphism