title: The influences of the recycle process on the bacterial community in a pilot scale microalgae raceway pond.
abstract: The use of recycled media has been shown to be a necessary step within the lifecycle of microalgal biofuels for economic sustainability and reducing the water footprint. However the impact of the harvesting of microalgae on the bacterial load of the recycled water has yet to be investigated. Within this study PCR-DGGE and real-time PCR was used to evaluate the bacterial community dynamics within the recycled water following harvest and concentration steps for a pilot scale open pond system (120,000L), which was developed for the production of green crude oil from Tetraselmis sp. in hyper saline water. Two stages were used in the harvesting; Stage 1 electroflocculation, and Stage 2 centrifugation. Electroflocculation was shown to have little effect on the bacterial cell concentration. In contrast bacterial diversity and cell concentration within the centrifugation step was greatly reduced.
authors.affiliation: School of Chemical Engineering, University of Adelaide, 5005, Australia; School of Applied Sciences, RMIT University, 3083, Australia. Electronic address: mason.erkelens@adelaide.edu.au.
authors.forename: Mason
authors.initials: M
authors.lastname: Erkelens
authors.affiliation: School of Applied Sciences, RMIT University, 3083, Australia.
authors.forename: Andrew S
authors.initials: AS
authors.lastname: Ball
authors.affiliation: School of Chemical Engineering, University of Adelaide, 5005, Australia.
authors.forename: David M
authors.initials: DM
authors.lastname: Lewis
mesh_terms: D001419:Bacteria / Q000254:growth & development*; D044822:Biodiversity; D000460:Chlorophyta / Q000378:metabolism; D015169:Colony Count, Microbial; D058645:Denaturing Gradient Gel Electrophoresis; D058086:Microalgae / Q000378:metabolism*; D010865:Pilot Projects; D060888:Real-Time Polymerase Chain Reaction; D059027:Recycling
keywords: Electroflocculation; Microalgae biofuels; Pilot scale; Recycle; Tetraselmis