title: The role of N-acyl homoserine lactones in maintaining the stability of aerobic granules.
abstract: In this study, porcine kidney acylase, as N-acyl homoserine lactones (AHLs)-degradation enzyme, was employed for the first time to directly investigate the role of AHLs in the structure stability of aerobic granules. Results clearly showed that inactivation of AHLs by AHLs-acylase could weaken the stability of aerobic granule. In the presence of AHLs-acylase, AHLs were degraded by hydrolyzing the amide linkage, which resulted in aerobic granular attachment potential and activity of AHLs-based quorum sensing significantly reduced. In addition, it was also found that inactivation of AHLs led to reduction of extracellular polysaccharides and protein (PN), especially PN, and induced extracellular polymeric substances matrix damaged, which was hostile to stability of aerobic granules. This study provided direct evidence that AHLs played a key role in improving aerobic granular stability, and a potential way to enhance long-term stability of aerobic granules.
authors.affiliation: School of Environment, Beijing Normal University, Beijing 100875, China; School of Water Resource and Environment, Shijiazhuang University of Economics, Hebei 050031, China.
authors.forename: Yaochen
authors.initials: Y
authors.lastname: Li
authors.affiliation: School of Environment, Beijing Normal University, Beijing 100875, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Hao
authors.affiliation: School of Environment, Beijing Normal University, Beijing 100875, China.
authors.forename: Junping
authors.initials: J
authors.lastname: Lv
authors.affiliation: School of Environment, Beijing Normal University, Beijing 100875, China.
authors.forename: Yaqin
authors.initials: Y
authors.lastname: Wang
authors.affiliation: School of Environment, Beijing Normal University, Beijing 100875, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Zhong
authors.affiliation: School of Environment, Beijing Normal University, Beijing 100875, China. Electronic address: zjrtshua@sohu.com.
authors.forename: Jianrong
authors.initials: J
authors.lastname: Zhu
mesh_terms: D054742:Acyl-Butyrolactones / Q000378:metabolism*; D000332:Aerobiosis; D000581:Amidohydrolases / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism; D001704:Biopolymers / Q000096:biosynthesis; D011134:Polysaccharides / Q000378:metabolism; D053038:Quorum Sensing; D012722:Sewage / Q000737:chemistry*
chemical_list: D054742:Acyl-Butyrolactones; D001426:Bacterial Proteins; D001704:Biopolymers; D011134:Polysaccharides; D012722:Sewage; D000581:Amidohydrolases
keywords: AHLs-acylase; Aerobic granules; Attachment potential; Extracellular polymeric substances; Quorum sensing