title: Erk1/2 signalling is involved in the differentiation of periodontal ligament stem cells to Schwann cells in dog.
abstract: Periodontal ligament stem cells (PDLSCs) show a multi-differentiation potential, however, the mechanism of its neural differentiation is still unclear. We aim to explore the potential role of Erk1/2 signalling pathway in the differentiation of PDLSCs to Schwann cells (SCs). When PDLSCs were induced to differentiate to SCs, the cells were treated with U0126, an Erk1/2 pathway specific inhibitor, and its potential effect on SC differentiation was appraised using Western-blotting, immunostaining, and reverse transcriptase PCR. Specifically inhibiting the Erk1/2 signalling pathway reduced the expression of SC-specific marker genes, S100, GFAP, and P75 and SC differentiation regulators, krox-20, Oct-6, and P0 in the cells undergoing induced differentiation from PDLSCs. From these results, we conclude that the Erk1/2 signalling pathway is involved in the induced differentiation of PDLSCs to SCs.
authors.affiliation: Department of Dentistry, Second Affiliated Hospital, School of Medicine, Zhejiang University, China.
authors.forename: Liao
authors.initials: L
authors.lastname: Dapeng
authors.affiliation: Department of Dentistry, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, China. Electronic address: lixiaojiekellylee@163.com.
authors.forename: Li
authors.initials: L
authors.lastname: Xiaojie
authors.affiliation: Dental Implant Center, West China College of Stomatology, Sichuan University, China.
authors.forename: Gong
authors.initials: G
authors.lastname: Ping
authors.affiliation: Department of Dentistry, Second Affiliated Hospital, School of Medicine, Zhejiang University, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Yan
authors.affiliation: Department of Dentistry, Second Affiliated Hospital, School of Medicine, Zhejiang University, China.
authors.forename: Sun
authors.initials: S
authors.lastname: Gang
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002454:Cell Differentiation / Q000502:physiology*; D004285:Dogs; D020935:MAP Kinase Signaling System / Q000502:physiology*; D010513:Periodontal Ligament / Q000166:cytology*; D016133:Polymerase Chain Reaction; D012583:Schwann Cells / Q000502:physiology*; D015398:Signal Transduction; D013234:Stem Cells / Q000166:cytology*
keywords: Differentiation; Erk1/2 signalling pathway; Periodontal ligament stem cells; Schwann cells