title: Rapid quantitative detection of Human immunodeficiency virus type 1 by a reverse transcription-loop-mediated isothermal amplification assay.
abstract: Accurate and rapid quantitation of Human immunodeficiency virus type 1 (HIV-1) RNA levels is a critical aspect in estimating the effect of antiviral therapy and establishing therapeutic schedule. Thus, for the first time, a rapid quantitative reverse transcription-loop-mediated isothermal amplification (RT-LAMP) was designed to quantitate HIV-1 RNA. The results showed that the dynamic range was from 2.5×10(2) to 10(7) copies with a coefficient of determination (R(2)) of 0.991, and the limit of detection of RT-LAMP by Probit analysis at the 95% detection level was 196 copies. The intra-assay coefficient of variation (CV) ranged from 0.67% to 2.08% at 10(7) copies and 7.25% to 12.97% at 250 copies. The CVs of inter-assay were 2.39% and 13.93% for the high and low copy numbers, respectively. No cross-reaction with Human immunodeficiency virus type 2 (HIV-2), Human T lymphotrophic virus type 1 (HTLV-1) and Hepatitis C virus (HCV) was observed and a good agreement between the RT-LAMP method and the real-time reverse transcription-polymerase chain reaction (qRT-PCR) test was achieved. This proposed RT-LAMP method could be useful for rapid diagnosis of high risk group and pharmacodynamic assessment of anti-HIV drug, especially in less-equipped laboratories of impoverished areas.
authors.affiliation: Key Laboratory of Medical Virology, Ministry of Health, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China; College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, China.
authors.forename: Yalan
authors.initials: Y
authors.lastname: Zeng
authors.affiliation: Key Laboratory of Medical Virology, Ministry of Health, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
authors.forename: Xiaoguang
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Medical Virology, Ministry of Health, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Nie
authors.affiliation: Key Laboratory of Medical Virology, Ministry of Health, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China; College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, China.
authors.forename: Xiong
authors.initials: X
authors.lastname: Ding
authors.affiliation: Institute of Genomic and Personalized Medicine, School of Life Science and Technology, Huazhong University of Science and Technology, 430074 Wuhan, China.
authors.forename: Brian Z
authors.initials: BZ
authors.lastname: Ring
authors.affiliation: Zhengzhou Center for Disease Control and Prevention, Zhengzhou, China.
authors.forename: Lanying
authors.initials: L
authors.lastname: Xu
authors.affiliation: Zhengzhou Center for Disease Control and Prevention, Zhengzhou, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Dai
authors.affiliation: Zhengzhou Center for Disease Control and Prevention, Zhengzhou, China.
authors.forename: Xiying
authors.initials: X
authors.lastname: Li
authors.affiliation: Zhengzhou Center for Disease Control and Prevention, Zhengzhou, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Ren
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, China. Electronic address: leishi@scut.edu.cn.
authors.forename: Lei
authors.initials: L
authors.lastname: Shi
authors.affiliation: Key Laboratory of Medical Virology, Ministry of Health, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China. Electronic address: maxj@ivdc.chinacdc.cn.
authors.forename: Xuejun
authors.initials: X
authors.lastname: Ma
mesh_terms: D002681:China; D017931:DNA Primers / Q000235:genetics; D015658:HIV Infections / Q000175:diagnosis* / Q000821:virology; D015497:HIV-1 / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D057230:Limit of Detection; D016014:Linear Models; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012367:RNA, Viral / Q000097:blood* / Q000235:genetics; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D017931:DNA Primers; D012367:RNA, Viral
keywords: Human immunodeficiency virus type 1 (HIV-1); Plasma; Quantitation; Reverse transcription-loop-mediated isothermal amplification (RT-LAMP)