title: Identification of differentially expressed serum proteins in infectious purpura fulminans.
abstract: Purpura fulminans (PF) is a life-threatening hemorrhagic condition. Because of the rarity and randomness of the disease, no improvement in treatment has been made for a long time. In this study, we assessed the serum proteome response to PF by comparing serum proteins between healthy controls and PF patient. Liquid chromatography with tandem mass spectrometry (LC-MS/MS) approach was used after depleting 6 abundant proteins of serum. In total, 262 proteins were confidently identified with 2 unique peptides, and 38 proteins were identified significantly up- (≥ 2) or downregulated (≤ 0.5) based on spectral counting ratios (SpCPF/N). In the 38 proteins with significant abundance changes, 11 proteins were previously known to be associated with burn or sepsis response, but 27 potentially novel proteins may be specifically associated with PF process. Two differentially expressed proteins, alpha-1-antitrypsin (SERPINA1) and alpha-2 antiplasmin (SERPINF2), were validated by Western blot. This is the first study where PF patient and healthy controls are compared in a proteomic study to elucidate proteins involved in the response to PF. This study provides an initial basis for future studies of PF, and the differentially expressed proteins might provide new therapeutic targets to decrease the mortality of PF.
authors.affiliation: Institute of Burn Research, Southwest Hospital, Third Military Medical University, State Key Laboratory of Trauma, Burns and Combined Injury, Chongqing Key Laboratory for Diseases Proteomics, Chongqing 400038, China.
authors.forename: Ting
authors.identifier: 0000-0002-1837-0128
authors.initials: T
authors.lastname: He
authors.affiliation: Department of Endocrinology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
authors.forename: Jiong-yu
authors.initials: JY
authors.lastname: Hu
authors.affiliation: Department of Obstetrics and Gynecology, Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing 400042, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Han
authors.affiliation: Institute of Burn Research, Southwest Hospital, Third Military Medical University, State Key Laboratory of Trauma, Burns and Combined Injury, Chongqing Key Laboratory for Diseases Proteomics, Chongqing 400038, China.
authors.forename: Dong-xia
authors.initials: DX
authors.lastname: Zhang
authors.affiliation: Institute of Burn Research, Southwest Hospital, Third Military Medical University, State Key Laboratory of Trauma, Burns and Combined Injury, Chongqing Key Laboratory for Diseases Proteomics, Chongqing 400038, China.
authors.forename: Xu-pin
authors.initials: XP
authors.lastname: Jiang
authors.affiliation: Department of Endocrinology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Chen
authors.affiliation: Institute of Burn Research, Southwest Hospital, Third Military Medical University, State Key Laboratory of Trauma, Burns and Combined Injury, Chongqing Key Laboratory for Diseases Proteomics, Chongqing 400038, China.
authors.forename: Yue-sheng
authors.initials: YS
authors.lastname: Huang
mesh_terms: D015415:Biomarkers / Q000097:blood; D002056:Burns / Q000097:blood* / Q000150:complications; D016022:Case-Control Studies; D005260:Female; D015870:Gene Expression; D063990:Gene Ontology; D006801:Humans; D008875:Middle Aged; D020543:Proteome / Q000235:genetics / Q000378:metabolism; D055665:Purpura Fulminans / Q000097:blood* / Q000382:microbiology; D018805:Sepsis / Q000097:blood* / Q000382:microbiology; D000515:alpha 1-Antitrypsin / Q000097:blood* / Q000235:genetics; D000979:alpha-2-Antiplasmin / Q000235:genetics / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D020543:Proteome; C043392:SERPINA1 protein, human; C534949:SERPINF2 protein, human; D000515:alpha 1-Antitrypsin; D000979:alpha-2-Antiplasmin