title: [The effect of RNA interfering TLR4 signal pathway on phagocytosis of Kupffer cells].
abstract: OBJECTIVE
  To investigate the effect of RNA interfering TLR4 signal pathway on phagocytosis of Kupffer cells.
  
  
  METHODS
  RAW2647 mice mononuclear macrophage leukemia cells were observed. The tested group was interfered by Tlr4-mus-1567 RNA which had the best result confirmed by QPCR, cells interfered by Negative Control RNA as NC group, and normal cell as control. We perform the phagocytosis test on each group.
  
  
  RESULTS
  The tested group has lower phagocytes percentage than control (17.67% +/- 3.51% vs 32.00% +/- 3.00%, P < 0.01), and lower phagocytic index (46.33% +/- 7.51% vs 82.00% +/- 6.08%, P < 0.01).
  
  
  CONCLUSIONS
  Decreased phagocytic activity was observed on Kupffer cells by RNA interference.
authors.forename: Shu-Fei
authors.initials: SF
authors.lastname: Zhang
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Yin-Lan
authors.initials: YL
authors.lastname: Liu
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Yang
authors.forename: Yan
authors.initials: Y
authors.lastname: Luo
authors.forename: Zhen-Jie
authors.initials: ZJ
authors.lastname: Zhuang
authors.forename: Qi-Bin
authors.initials: QB
authors.lastname: Jiao
authors.forename: Jian-Yu
authors.initials: JY
authors.lastname: Chen
authors.forename: Dong-Xue
authors.initials: DX
authors.lastname: Bian
authors.forename: Xiao-Jie
authors.initials: XJ
authors.lastname: Ma
authors.forename: Yun-Hao
authors.initials: YH
authors.lastname: Xun
authors.forename: Ming-Li
authors.initials: ML
authors.lastname: Zhu
authors.forename: Jun-Ping
authors.initials: JP
authors.lastname: Shi
mesh_terms: D000818:Animals; D007728:Kupffer Cells / Q000276:immunology*; D051379:Mice; D010587:Phagocytosis; D034622:RNA Interference; D015398:Signal Transduction; D051197:Toll-Like Receptor 4 / Q000235:genetics* / Q000276:immunology*
chemical_list: C493487:Tlr4 protein, mouse; D051197:Toll-Like Receptor 4