title: Systemic inflammation promotes lung metastasis via E-selectin upregulation in mouse breast cancer model.
abstract: Systemic inflammation might modulate the microenvironment in the lungs and promotes metastasis. E-selectin, an inflammation inducible endothelial cell adhesion molecule, has been reported to play an important role in homing metastatic cancer cells. To study the effects of E-selectin expression induced by systemic inflammation on breast cancer metastasis, we first treated BALB/c mice with lipopolysaccharide (LPS) to induce systemic inflammation. Pulmonary tissues were analyzed by wet/dry ratio, hematoxylin and eosin (H&E) staining and immunohistochemistry. Then 4T1 cells were injected via tail vein. Lung surface metastasis was counted and detected by histological analysis. LPS-induced E-selectin expression and tumor cells adhesion were assessed by western blotting and immunofluorescence. The circulating levels of proinflammatory cytokines in sera were evaluated by ELISA. Our results showed that a significant increase in breast cancer metastasis to lungs was observed in LPS-treated mice vs. the PBS-treated mice, accompanying with an increased E-selectin expression in pulmonary tissue of LPS-treated mice. In vitro studies showed a significant elevation of E-selectin production in MPVECs which enhanced the adhesion activity of 4T1 cells. Treatment with anti-E-selectin antibody significantly reduced the development of metastasis in vivo, and significantly reduced the adhesion of 4T1 cells to MPVECs in vitro. Our results suggest that systemic inflammation may increase the expression of E-selectin which mediated the lung metastasis of breast cancer in mouse model.
authors.affiliation: Cancer Therapy and Research Center; Shandong Provincial Hospital; Shandong University; Jinan, PR China.
authors.forename: Man
authors.initials: M
authors.lastname: Jiang
authors.affiliation: Cancer Therapy and Research Center; Shandong Provincial Hospital; Shandong University; Jinan, PR China.
authors.forename: Xiaoya
authors.initials: X
authors.lastname: Xu
authors.affiliation: Cancer Therapy and Research Center; Shandong Provincial Hospital; Shandong University; Jinan, PR China.
authors.forename: Yuli
authors.initials: Y
authors.lastname: Bi
authors.affiliation: Cancer Therapy and Research Center; Shandong Provincial Hospital; Shandong University; Jinan, PR China.
authors.forename: Jiying
authors.initials: J
authors.lastname: Xu
authors.affiliation: Cancer Therapy and Research Center; Shandong Provincial Hospital; Shandong University; Jinan, PR China.
authors.forename: Chengyong
authors.initials: C
authors.lastname: Qin
authors.affiliation: Cancer Therapy and Research Center; Shandong Provincial Hospital; Shandong University; Jinan, PR China.
authors.forename: Mingyong
authors.initials: M
authors.lastname: Han
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D018920:Coculture Techniques; D019040:E-Selectin / Q000235:genetics* / Q000378:metabolism; D004730:Endothelium, Vascular / Q000473:pathology; D005260:Female; D015870:Gene Expression; D006801:Humans; D007249:Inflammation / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008175:Lung Neoplasms / Q000276:immunology* / Q000378:metabolism / Q000556:secondary; D008325:Mammary Neoplasms, Experimental / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation; D011651:Pulmonary Artery / Q000473:pathology; D015854:Up-Regulation
chemical_list: D019040:E-Selectin; D008070:Lipopolysaccharides
keywords: E-selectin; LPS; breast cancer; inflammation; lung; metastasis; microenvironment