title: [Preparation and properties evaluation of methacrylic alginate gell beads cross-linked by mixed metal-cation].
abstract: The aim of this study was to obtain the alginate gels which could have proper compressive strength and excellent permeability for cell proliferation and could have more promising potentials in the application of tissue engineering. Through the reaction of the carboxyl of the alginate and the amino of methacrylic acid, methylacrylic was generated into alginate long chain which could be enhanced by the polymerization of double bond under thermal reaction condition. And then alginate gel beads were prepared using the mixture of calcium chloride and barium chloride solution as cationic crosslinker, and the compressive modulus and permeability of the prepared alginate gel beads were investigated. When the ratio of barium ions to calcium ions was 5:5, the compression modulus was 189.7 kPa, and it showed the best permeability for trypsin with molecular weight of 24 kDa and entrapment effect for bovine serum albumin with molecular weight of 67 kDa. Compared to compositions of other ratios, the alginate gel beads made in 5:5 mixture indicated excellent compressive modulus and permeability. These results indicated that the alginate hydrogel beads with the ratio of barium ions to calcium ions being 5:5 have a potential application in tissue engineering as a support material and encapsulating materials in cell culture.
authors.forename: Xing
authors.initials: X
authors.lastname: Fan
authors.forename: Shengnan
authors.initials: S
authors.lastname: Lei
authors.forename: Liling
authors.initials: L
authors.lastname: Ren
mesh_terms: D000464:Alginates / Q000737:chemistry*; D017609:Barium Compounds / Q000737:chemistry; D002122:Calcium Chloride / Q000737:chemistry; D002412:Cations; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D002712:Chlorides / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000737:chemistry; D005782:Gels / Q000737:chemistry; D008670:Metals; D011109:Polymethacrylic Acids / Q000737:chemistry*; D023822:Tissue Engineering
chemical_list: D000464:Alginates; D017609:Barium Compounds; D002412:Cations; D002712:Chlorides; D003432:Cross-Linking Reagents; D005782:Gels; D008670:Metals; D011109:Polymethacrylic Acids; C024986:barium chloride; D002122:Calcium Chloride