title: Strawberry intake increases blood fluid, erythrocyte and mononuclear cell defenses against oxidative challenge.
abstract: The health promoting effects of a regular consumption of strawberries deserve attention, and a direct or indirect antioxidant role of strawberry bioactive compounds is among the most probable mechanisms underlying their beneficial properties. In the present study, we evaluated the overall effects of a 2-week daily consumption of strawberries on plasma antioxidant status, membrane lipid susceptibility to ex vivo-induced oxidation, and erythrocyte and mononuclear cell resistance to oxidative damage in apparently healthy volunteers. After strawberry intake, a moderate increase in fasting plasma antioxidant capacity and vitamin C was observed, together with a significant increase in the lag phase preceding plasma lipid oxidation. A significantly enhanced resistance to oxidative hemolysis was confirmed in red blood cells, while no significant changes were found in the extent of their membrane lipid peroxidation. For the first time, increased intake of strawberries for only 2weeks was shown to be sufficient to attenuate mononuclear cell mortality after ex vivo exposure to a single acuteoxidative challenge, but the analysis of DNA oxidative damage gave conflicting results. These findings suggest that a regular consumption of strawberries may enhance body defences against oxidative challenges.
authors.affiliation: Dipartimento di Science Cliniche Specialistiche ed Odontostomatologiche, Facoltà di Medicina, Università Politecnica delle Marche, Ancona, Italy.
authors.forename: Sara
authors.initials: S
authors.lastname: Tulipani
authors.affiliation: Dipartimento di Science Cliniche Specialistiche ed Odontostomatologiche, Facoltà di Medicina, Università Politecnica delle Marche, Ancona, Italy.
authors.forename: Tatiana
authors.initials: T
authors.lastname: Armeni
authors.affiliation: Dipartimento di Science Cliniche Specialistiche ed Odontostomatologiche, Facoltà di Medicina, Università Politecnica delle Marche, Ancona, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Giampieri
authors.affiliation: Dipartimento di Science Cliniche Specialistiche ed Odontostomatologiche, Facoltà di Medicina, Università Politecnica delle Marche, Ancona, Italy.
authors.forename: José M
authors.initials: JM
authors.lastname: Alvarez-Suarez
authors.affiliation: Grupo de Investigación en Polifenoles (GIP-USAL), Faculty of Pharmacy, Salamanca University, Campus Miguel de Unamuno, E-37007 Salamanca, Spain.
authors.forename: Ana M
authors.initials: AM
authors.lastname: Gonzalez-Paramás
authors.affiliation: Grupo de Investigación en Polifenoles (GIP-USAL), Faculty of Pharmacy, Salamanca University, Campus Miguel de Unamuno, E-37007 Salamanca, Spain.
authors.forename: Celestino
authors.initials: C
authors.lastname: Santos-Buelga
authors.affiliation: Laboratory of Torrette-Umberto I Regional Hospital, Ancona, Italy.
authors.forename: Franco
authors.initials: F
authors.lastname: Busco
authors.affiliation: Dipartimento di Science Cliniche Specialistiche ed Odontostomatologiche, Facoltà di Medicina, Università Politecnica delle Marche, Ancona, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Principato
authors.affiliation: Dipartimento di Scienze Biomediche e Sanità Pubblica, Facoltà di Medicina, Università Politecnica delle Marche, Ancona, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Bompadre
authors.affiliation: Instituto de Nutricion y Tecnologia de Alimentos (INYTA), Department of Physiology, Universidad de Granada, Granada, Spain.
authors.forename: José L
authors.initials: JL
authors.lastname: Quiles
authors.affiliation: Dipartimento di Scienze Agrarie, Alimentari ed Ambientali (D3A), Università Politecnica delle Marche, Ancona, Italy.
authors.forename: Bruno
authors.initials: B
authors.lastname: Mezzetti
authors.affiliation: Dipartimento di Science Cliniche Specialistiche ed Odontostomatologiche, Facoltà di Medicina, Università Politecnica delle Marche, Ancona, Italy. Electronic address: m.a.battino@univpm.it.
authors.forename: Maurizio
authors.initials: M
authors.lastname: Battino
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000032:analysis / Q000378:metabolism*; D001205:Ascorbic Acid / Q000097:blood; D004249:DNA Damage; D004912:Erythrocytes / Q000378:metabolism*; D005260:Female; D031985:Fragaria / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008297:Male; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D055815:Young Adult
chemical_list: D000975:Antioxidants; D001205:Ascorbic Acid
keywords: Antihaemolytic effects; DNA damage; Human study; Mononuclear cell mortality; Plasma antioxidant status; Strawberry