title: Evaluation of storage conditions on equine adipose tissue-derived multipotent mesenchymal stromal cells.
abstract: The transplantation of multipotent mesenchymal stromal cells (MSCs) is a potentially promising therapy for the treatment of tendon and ligament injuries and some forms of articular pathology in horses. This study investigated the effects of storage conditions on MSCs. Equine adipose tissue-derived MSCs (eAd-MSCs) were stored at 4 °C and at room temperature (RT) for 24 and 48 h, and viability, doubling time, expression of CD44 and CD90 antigens, clonogenic/differentiation potentials, and karyotype were subsequently evaluated. The eAd-MSC viability was significantly affected by the storage conditions, while doubling time was not significantly altered. The findings indicate (1) that storage at 4 °C is preferable to RT as this results in greater numbers of viable, morphologically normal cells, and (2) that cells should be used within 24 h.
authors.affiliation: Department of Veterinary Medicine, University of Perugia - Via San Costanzo, 4, 06126 Perugia, Italy. Electronic address: francesca.mercati@unipg.it.
authors.forename: Francesca
authors.initials: F
authors.lastname: Mercati
authors.affiliation: Department of Veterinary Medicine, University of Perugia - Via San Costanzo, 4, 06126 Perugia, Italy.
authors.forename: Luisa
authors.initials: L
authors.lastname: Pascucci
authors.affiliation: Experimental Zooprophylactic Institute of Umbria and Marche, Via G. Salvemini, 1, 06126 Perugia, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Curina
authors.affiliation: School of Biosciences and Veterinary Medicine, University of Camerino, Via Gentile III da Varano, 62032 Camerino (MC), Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Scocco
authors.affiliation: School of Biosciences and Veterinary Medicine, University of Camerino, Via Gentile III da Varano, 62032 Camerino (MC), Italy.
authors.forename: Federico Maria
authors.initials: FM
authors.lastname: Tardella
authors.affiliation: Department of Veterinary Medicine, University of Perugia - Via San Costanzo, 4, 06126 Perugia, Italy.
authors.forename: Cecilia
authors.initials: C
authors.lastname: Dall'aglio
authors.affiliation: Experimental Zooprophylactic Institute of Umbria and Marche, Via G. Salvemini, 1, 06126 Perugia, Italy.
authors.forename: Carla
authors.initials: C
authors.lastname: Marini
authors.affiliation: Department of Veterinary Medicine, University of Perugia - Via San Costanzo, 4, 06126 Perugia, Italy.
authors.forename: Piero
authors.initials: P
authors.lastname: Ceccarelli
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D000818:Animals; D002454:Cell Differentiation; D002470:Cell Survival; D015925:Cryopreservation / Q000662:veterinary*; D006736:Horses; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D039902:Multipotent Stem Cells / Q000166:cytology*; D013696:Temperature; D013997:Time Factors
keywords: Adipose tissue; Horse; Mesenchymal stem cells; Multipotent; Transport