title: Comparative study of Microsporum canis isolates by DNA fingerprinting.
abstract: Microsporum canis is a zoophilic fungus and it is an important agent of dermatophytosis. Cats act as important reservoirs. Clinically, it is too difficult to differentiate dermatophytosis caused by various species, also this fungus loses its morphological characteristics easily because of subculture; so using of rapid and accurate laboratory techniques for identifying the dermatophytes is important, therefore, RAPD-PCR was applied for the differentiation of the isolates. In this study, 10 M. canis isolates were detected in cats, dog, human, fox and rabbit at the Mycology Research Center, Faculty of Veterinary Medicine, University of Tehran. For running the RAPD-PCR, PCR set system and three random primers OPU 15, OPU 13 and OPA 04 were used. Then phylogenetic tree and similarity coefficient table were drawn. The results showed that there were some common bands between M. canis isolates. There were some specific bands for each isolates, as well. Our study showed, despite the typical morphology of the whole isolates, they were placed in different branches in molecular typing.
authors.affiliation: Mycology Research Center, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
authors.forename: Shabnam
authors.initials: S
authors.lastname: Shafiee
authors.forename: Ali Reza
authors.initials: AR
authors.lastname: Khosravi
authors.forename: Iradj
authors.initials: I
authors.lastname: Ashrafi Tamai
mesh_terms: D000820:Animal Diseases / Q000382:microbiology; D000818:Animals; D000935:Antifungal Agents / Q000494:pharmacology; D002415:Cats; D016000:Cluster Analysis; D016172:DNA Fingerprinting; D004271:DNA, Fungal; D003881:Dermatomycoses / Q000382:microbiology; D004285:Dogs; D005589:Foxes; D005838:Genotype; D006801:Humans; D008826:Microbial Sensitivity Tests; D008864:Microsporum / Q000145:classification* / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D011817:Rabbits
chemical_list: D000935:Antifungal Agents; D004271:DNA, Fungal
keywords: DNA fingerprinting; Dermatophytosis; Microsporum canis; isolates differentiation