title: Transcriptional analysis of the porcine TTID gene and association of different TTID genotypes with carcass traits.
abstract: The titin immunoglobulin domain (TTID) protein localizes to the Z line in muscle and binds to alpha-actinin and gamma-filamin. It plays an indispensable role in stabilizing and anchoring of thin filaments. In this study, the 5'-regulatory region of the porcine TTID gene was analyzed with bioinformatic methods. Another objective of this study was to further investigate the polymorphism in the intron 6 of the porcine TTID gene. We determined allele frequency among six Chinese porcine purebreds. The polymorphisms were genotyped in a population of 280 F2 pigs representing two Large White x Meishan reference families. Different TTID genotypes were significantly associated with carcass traits, including skin percentage (P < 0.05), loin eye area (P < 0.05), and average skin thickness (P < 0.01). Our study will continue to lay the groundwork for further investigations into the detailed function of the porcine TTID gene.
authors.affiliation: Department of Bio-Engineering, Faculty of Environmental Engineering, Wuhan Textile University, Wuhan, Hubei Province, China.
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.affiliation: Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture, Key Laboratory of Agricultural Animal Genetics and Breeding, Ministry of Education, Huazhong Agricultural University, Wuhan, Hubei Province, China.
authors.forename: Y-P
authors.initials: YP
authors.lastname: Feng
authors.affiliation: Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture, Key Laboratory of Agricultural Animal Genetics and Breeding, Ministry of Education, Huazhong Agricultural University, Wuhan, Hubei Province, China.
authors.forename: B
authors.initials: B
authors.lastname: Zuo
authors.affiliation: Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture, Key Laboratory of Agricultural Animal Genetics and Breeding, Ministry of Education, Huazhong Agricultural University, Wuhan, Hubei Province, China.
authors.forename: Y-Z
authors.initials: YZ
authors.lastname: Xiong
authors.affiliation: Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture, Key Laboratory of Agricultural Animal Genetics and Breeding, Ministry of Education, Huazhong Agricultural University, Wuhan, Hubei Province, China wes2100@webmail.hzau.edu.cn.
authors.forename: C-Y
authors.initials: CY
authors.lastname: Deng
mesh_terms: D024506:5' Flanking Region; D000822:Animal Husbandry; D000818:Animals; D001483:Base Sequence; D001823:Body Composition; D064211:Connectin / Q000235:genetics*; D005787:Gene Frequency; D056726:Genetic Association Studies / Q000379:methods*; D005838:Genotype; D016018:Least-Squares Analysis; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci; D013552:Swine
chemical_list: D064211:Connectin