title: Crystallization and preliminary X-ray crystallographic analysis of latent isoform PPO4 mushroom (Agaricus bisporus) tyrosinase.
abstract: Tyrosinase exhibits catalytic activity for the ortho-hydroxylation of monophenols to diphenols as well as their subsequent oxidation to quinones. Owing to polymerization of these quinones, brown-coloured high-molecular-weight compounds called melanins are generated. The latent precursor form of polyphenol oxidase 4, one of the six tyrosinase isoforms from Agaricus bisporus, was purified to homogeneity and crystallized. The obtained crystals belonged to space group C121 (two molecules per asymmetric unit) and diffracted to 2.78 Å resolution. The protein only formed crystals under low-salt conditions using the 6-tungstotellurate(VI) salt Na6[TeW6O24] · 22H2O as a co-crystallization agent.
authors.affiliation: Institut für Biophysikalische Chemie, Universität Wien, Althanstrasse 14, 1090 Wien, Austria.
authors.forename: Stephan Gerhard
authors.initials: SG
authors.lastname: Mauracher
authors.affiliation: Institut für Biophysikalische Chemie, Universität Wien, Althanstrasse 14, 1090 Wien, Austria.
authors.forename: Christian
authors.initials: C
authors.lastname: Molitor
authors.affiliation: School of Engineering and Science, Jacobs University, PO Box 750 561, 28759 Bremen, Germany.
authors.forename: Rami
authors.initials: R
authors.lastname: Al-Oweini
authors.affiliation: School of Engineering and Science, Jacobs University, PO Box 750 561, 28759 Bremen, Germany.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Kortz
authors.affiliation: Institut für Biophysikalische Chemie, Universität Wien, Althanstrasse 14, 1090 Wien, Austria.
authors.forename: Annette
authors.initials: A
authors.lastname: Rompel
mesh_terms: D000364:Agaricus / Q000201:enzymology*; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods*; D007527:Isoenzymes / Q000737:chemistry*; D011487:Protein Conformation; D014443:Tyrosine / Q000737:chemistry*
chemical_list: D007527:Isoenzymes; D014443:Tyrosine
keywords: Agaricus bisporus; polyoxometalates; polyphenol oxidase 4; tyrosinases; zymogens