title: Embryonic thermosensitive TRPA1 determines transgenerational diapause phenotype of the silkworm, Bombyx mori.
abstract: In the bivoltine strain of the silkworm, Bombyx mori, embryonic diapause is induced transgenerationally as a maternal effect. Progeny diapause is determined by the environmental temperature during embryonic development of the mother; however, its molecular mechanisms are largely unknown. Here, we show that the Bombyx TRPA1 ortholog (BmTrpA1) acts as a thermosensitive transient receptor potential (TRP) channel that is activated at temperatures above ∼ 21 °C and affects the induction of diapause in progeny. In addition, we show that embryonic RNAi of BmTrpA1 affects diapause hormone release during pupal-adult development. This study identifying a thermosensitive TRP channel that acts as a molecular switch for a relatively long-term predictive adaptive response by inducing an alternative phenotype to seasonal polyphenism is unique.
authors.affiliation: Faculty of Textile Science and Technology, Shinshu University, Ueda 386-8567, Japan.
authors.forename: Azusa
authors.initials: A
authors.lastname: Sato
authors.forename: Takaaki
authors.initials: T
authors.lastname: Sokabe
authors.forename: Makiko
authors.initials: M
authors.lastname: Kashio
authors.forename: Yuji
authors.initials: Y
authors.lastname: Yasukochi
authors.forename: Makoto
authors.initials: M
authors.lastname: Tominaga
authors.forename: Kunihiro
authors.initials: K
authors.lastname: Shiomi
mesh_terms: D000818:Animals; D001835:Body Weight; D012831:Bombyx / Q000196:embryology* / Q000235:genetics / Q000378:metabolism*; D064666:Diapause, Insect / Q000235:genetics*; D004625:Embryo, Nonmammalian / Q000378:metabolism*; D047108:Embryonic Development / Q000235:genetics; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D055785:Gene Knockdown Techniques; D057809:HEK293 Cells; D006801:Humans; D040582:Inheritance Patterns / Q000235:genetics*; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism*; D015640:Ion Channel Gating; D008969:Molecular Sequence Data; D055099:Neuroendocrine Cells / Q000166:cytology / Q000378:metabolism; D009479:Neuropeptides / Q000378:metabolism; D010641:Phenotype; D011679:Pupa / Q000166:cytology / Q000378:metabolism; D034622:RNA Interference; D050052:TRPC Cation Channels / Q000235:genetics / Q000378:metabolism*; D013696:Temperature
chemical_list: D019476:Insect Proteins; D009479:Neuropeptides; D050052:TRPC Cation Channels; C075027:diapause hormone