title: Simultaneous determination of 12 illicit drugs in whole blood and urine by solid phase extraction and UPLC-MS/MS.
abstract: A rapid and sensitive method based on solid phase extraction and ultra performance liquid chromatography-electrospray ionization tandem mass spectrometry (UPLC-ESI-MS/MS) for the simultaneous determination of amphetamine, methamphetamine, 3,4-methylenedioxyamphetamine, 3,4-methylene-dioxymethamphetamine, N-methyl-1-(3,4-methyl-enedioxyphenyl)-2-butanamine, 3,4-methylenedioxyethylamphetamine, p-methoxymethamphetamine, ephedrine, N-methylephedrine, cathinone, methcathinone, and ketamine in whole blood and urine was developed and validated. Following solid phase extraction, the analytes were separated on ACQUITY UPLC BEH Phenyl column (100mm×2.1mm, 1.7μm) under gradient elution using a mobile phase containing of acetonitrile and 0.3% formic acid in water at a flow rate of 0.4mLmin(-1) and analyzed by a triplequadrupole mass spectrometer in the multiple reaction monitoring (MRM) mode. The proposed method was linear for each analyte with correlation coefficients over 0.99. Recovery validation studies showed accuracy bias below 4.4%. Acceptable precision was also obtained with a relative standard deviation below 8.9%. The sensitivity of the assay was found to be adequate for the quantitation of the illicit drugs in whole blood and urine sample and was higher than reported methods. The present method was proved to be reliable and robust for drug screening in forensic toxicological analysis.
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University, Shijiazhuang, Hebei Province 050017, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Procuratoral Technology and Information Research Center, Supreme People's Procuratorate, Beijing 100144, China.
authors.forename: Zhao-Hong
authors.initials: ZH
authors.lastname: Wang
authors.affiliation: Key Laboratory of Narcotics Assay and Control Technology, Ministry of Public Security, People's of China, Kunming, Yunnan Province 650000, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Li
authors.affiliation: Procuratoral Technology and Information Research Center, Supreme People's Procuratorate, Beijing 100144, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Procuratoral Technology and Information Research Center, Supreme People's Procuratorate, Beijing 100144, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Zhao
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University, Shijiazhuang, Hebei Province 050017, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Key Laboratory of Narcotics Assay and Control Technology, Ministry of Public Security, People's of China, Kunming, Yunnan Province 650000, China.
authors.forename: Wen-Song
authors.initials: WS
authors.lastname: Zhao
mesh_terms: D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D006801:Humans; D013287:Illicit Drugs / Q000097:blood* / Q000737:chemistry / Q000302:isolation & purification / Q000652:urine*; D016014:Linear Models; D008297:Male; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry / Q000379:methods*; D055815:Young Adult
chemical_list: D013287:Illicit Drugs
keywords: Illicit drugs; Solid phase extraction; UPLC–MS/MS; Urine; Whole blood