title: Molecular and epidemiological data on Anisakis spp. (Nematoda: Anisakidae) in commercial fish caught off northern Sardinia (western Mediterranean Sea).
abstract: Anisakiasis is a fish-borne zoonosis caused by third stage larvae of the nematode Anisakis sp. present in fish or cephalopods. This is the first contribution to the molecular identification and epidemiology of Anisakis spp. in commercial fish from the Gulf of Asinara (Sardinia, western Mediterranean Sea). Between April 2006 to November 2011, 777 specimens of 10 fish species (Engraulis encrasicolus, Merluccius merluccius, Micromesistius poutassou, Phycis blennoides, Sardina pilchardus, Sardinella aurita, Scomber colias, Sphyraena viridensis, Trachurus mediterraneus, Trachurus trachurus) were examined for Anisakis sp. larvae. A total of 1286 larvae were found in 218 fish. The great majority of larvae were located in the body cavity, and only a small part (60, 4.7%) in the muscle. All the Type I larvae (1272) were identified as Anisakis pegreffii and all the Type II (14) as Anisakis physeteris, confirming that A. pegreffii is the dominant species and the most important agent of human anisakiasis in the western Mediterranean Sea.
authors.affiliation: Dipartimento di Medicina Veterinaria, Parassitologia e Malattie Parassitarie, Università di Sassari, Via Vienna, 2, 07100 Sassari, Italy.
authors.forename: M C
authors.initials: MC
authors.lastname: Piras
authors.affiliation: Laboratorio di Ispezione Alimenti, Istituto Zooprofilattico Sperimentale della Sardegna, Via Duca degli Abruzzi, 8, 07100 Sassari, Italy.
authors.forename: T
authors.initials: T
authors.lastname: Tedde
authors.affiliation: Dipartimento di Medicina Veterinaria, Parassitologia e Malattie Parassitarie, Università di Sassari, Via Vienna, 2, 07100 Sassari, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Garippa
authors.affiliation: Laboratorio di Ispezione Alimenti, Istituto Zooprofilattico Sperimentale della Sardegna, Via Duca degli Abruzzi, 8, 07100 Sassari, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Virgilio
authors.affiliation: Dipartimento di Scienze della Natura e del Territorio, Sezione di Zoologia, Archeozoologia e Genetica, Università di Sassari, Via Muroni, 25, 07100 Sassari, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Sanna
authors.affiliation: Institute of Applied Sciences and Technology of Gabes, Rue Amor Ben El Khatab, Gabes, Tunisia.
authors.forename: S
authors.initials: S
authors.lastname: Farjallah
authors.affiliation: Dipartimento di Medicina Veterinaria, Parassitologia e Malattie Parassitarie, Università di Sassari, Via Vienna, 2, 07100 Sassari, Italy. Electronic address: malpar@uniss.it.
authors.forename: P
authors.initials: P
authors.lastname: Merella
mesh_terms: D000818:Animals; D017129:Anisakiasis / Q000453:epidemiology*; D017163:Anisakis / Q000502:physiology; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D005393:Fish Diseases / Q000453:epidemiology* / Q000469:parasitology; D005398:Fisheries; D005399:Fishes; D006790:Host-Parasite Interactions; D007558:Italy; D007814:Larva; D008522:Mediterranean Sea / Q000453:epidemiology; D009132:Muscles / Q000469:parasitology; D015995:Prevalence
chemical_list: D021903:DNA, Ribosomal Spacer
keywords: Anisakis; ITS; Mediterranean Sea; PCR-RFLP; Sardinia; Zoonosis