title: Expression of budding uninhibited by benzimidazoles-1 and mitotic arrest deficient-2 in endometrial carcinoma and its significance.
abstract: OBJECTIVE
  The aim of this study was to explore the expression of budding uninhibited by benzimidazoles-1 (Bub1) and mitotic arrest deficient-2 (Mad2) in endometrial carcinoma and its significance.
  
  
  METHODS
  The expression of Bub 1 and Mad2 in 30 human normal endometrial tissues (group A), 30 complexly-hyperplastic endometrial tissues (group B), and 63 endometrial carcinoma tissues (group C) was observed using immunohistochemistry (the streptavidin-peroxidase method).
  
  
  RESULTS
  The positive expression rates of Bub1 in groups A, B, and C were 86.67%, 56.67%, and 28.57%, respectively. The positive rate of Bub1 protein was correlated with the differentiation degree and clinical stage of endometrial carcinoma (p < 0.05) other than lymph node metastasis (p > 0.05): A higher differentiation degree and a more advanced stage of endometrial carcinoma indicated a higher positive rate of Bub1 protein. The positive rates of Mad2 protein in groups A, B, and C were 23.33%, 56.67%, and 85.71%, respectively. The positive rate of Mad2 protein was correlated with the differentiation degree of endometrial carcinoma (p < 0.05) other than its clinical stage and lymph node metastasis (p > 0.05): A lower differentiation degree indicated a higher positive rate of Mad2 protein. Bub1 and Mad2 proteins were negatively correlated in the endometrial carcinoma tissues (r = - 0.719, p < 0.001).
  
  
  CONCLUSIONS
  Bub1 and Mad2 proteins interact with each other. They may play an important role in the initiation and development of endometrial carcinoma.
authors.forename: Q
authors.initials: Q
authors.lastname: Zhao
authors.forename: A P
authors.initials: AP
authors.lastname: Bian
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.forename: L
authors.initials: L
authors.lastname: Qin
authors.forename: H R
authors.initials: HR
authors.lastname: Shi
authors.forename: K
authors.initials: K
authors.lastname: Su
mesh_terms: D000328:Adult; D016889:Endometrial Neoplasms / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D064246:Mad2 Proteins / Q000096:biosynthesis* / Q000378:metabolism; D008875:Middle Aged; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis* / Q000378:metabolism; D018709:Statistics, Nonparametric
chemical_list: C120552:MAD2L2 protein, human; D064246:Mad2 Proteins; C584277:BUB1 protein, human; D017346:Protein Serine-Threonine Kinases