title: Identification of cell membrane protein stress-induced phosphoprotein 1 as a potential ovarian cancer biomarker using aptamers selected by cell systematic evolution of ligands by exponential enrichment.
abstract: In this paper, we describe the elucidation of the target of an aptamer against ovarian cancer previously obtained by cell-SELEX (SELEX = systematic evolution of ligands by exponential enrichment). The target's identity, stress-induced phosphoprotein 1 (STIP1), was determined by mass spectrometry and validated by flow cytometry, using siRNA silencing and protein blotting. Initial oncologic studies show that the aptamer inhibits cell invasion, indicating that STIP1, which is currently under investigation as a potential biomarker for ovarian cancer, plays a critical role in this process. These results serve as an excellent example of how protein target identification of aptamers obtained by cell-SELEX can serve as a means to identify promising biomarker candidates and can promote the development of aptamers as a new drug class to block important oncological processes.
authors.affiliation: Center for Research at Bio/Nano Interface, Departments of Chemistry and of Physiology and Functional Genomics, Shands Cancer Center, UF Genetics Institute and McKnight Brain Institute, University of Florida , Gainesville, Florida 32611-7200, United States.
authors.forename: Dimitri
authors.initials: D
authors.lastname: Van Simaeys
authors.forename: Diane
authors.initials: D
authors.lastname: Turek
authors.forename: Carole
authors.initials: C
authors.lastname: Champanhac
authors.forename: Julia
authors.initials: J
authors.lastname: Vaizer
authors.forename: Kwame
authors.initials: K
authors.lastname: Sefah
authors.forename: Jing
authors.initials: J
authors.lastname: Zhen
authors.forename: Rebecca
authors.initials: R
authors.lastname: Sutphen
authors.forename: Weihong
authors.initials: W
authors.lastname: Tan
mesh_terms: D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000378:metabolism*; D017931:DNA Primers; D005260:Female; D006801:Humans; D008024:Ligands; D010051:Ovarian Neoplasms / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics; D052156:SELEX Aptamer Technique
chemical_list: D014408:Biomarkers, Tumor; D017931:DNA Primers; D008024:Ligands; D034741:RNA, Small Interfering