title: Novel methods based on (13)C detection to study intrinsically disordered proteins.
abstract: Intrinsically disordered proteins (IDPs) are characterized by highly flexible solvent exposed backbones and can sample many different conformations. These properties confer them functional advantages, complementary to those of folded proteins, which need to be characterized to expand our view of how protein structural and dynamic features affect function beyond the static picture of a single well defined 3D structure that has influenced so much our way of thinking. NMR spectroscopy provides a unique tool for the atomic resolution characterization of highly flexible macromolecules in general and of IDPs in particular. The peculiar properties of IDPs however have profound effects on spectroscopic parameters. It is thus worth thinking about these aspects to make the best use of the great potential of NMR spectroscopy to contribute to this fascinating field of research. In particular, after many years of dealing with exclusively heteronuclear NMR experiments based on (13)C direct detection, we would like here to address their relevance when studying IDPs.
authors.affiliation: Magnetic Resonance Center and Department of Chemistry "Ugo Schiff", University of Florence, Via L. Sacconi 6, 50019 Sesto Fiorentino, Italy. Electronic address: felli@cerm.unifi.it.
authors.forename: Isabella C
authors.initials: IC
authors.lastname: Felli
authors.affiliation: Magnetic Resonance Center and Department of Chemistry "Ugo Schiff", University of Florence, Via L. Sacconi 6, 50019 Sesto Fiorentino, Italy. Electronic address: pierattelli@cerm.unifi.it.
authors.forename: Roberta
authors.initials: R
authors.lastname: Pierattelli
mesh_terms: D000818:Animals; D002247:Carbon Isotopes; D006801:Humans; D064267:Intrinsically Disordered Proteins / Q000737:chemistry*; D007553:Isotope Labeling; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011487:Protein Conformation
chemical_list: D002247:Carbon Isotopes; D064267:Intrinsically Disordered Proteins
keywords: 13C direct detection; IDP; Intrinsically disordered proteins; NMR