title: Halolamina rubra sp. nov., a haloarchaeon isolated from non-purified solar salt.
abstract: Two Gram-stain negative, rod-shaped and motile extreme halophiles, designated CBA1107(T) and CBA1108, were isolated from non-purified solar salt. Based on the phylogenetic analysis, strains CBA1107(T) and CBA1108 were shown to belong to the genus Halolamina, with similarities for the 16S rRNA gene sequences between strains CBA1107(T) and Halolamina pelagica TBN21(T) , Halolamina salina WSY15-H3(T) and Halolamina salifodinae WSY15-H1(T) of 98.3, 97.6 and 97.3 %, respectively; the similarities for the rpoB' gene sequences between the same strains were 96.0, 95.3 and 94.6 %, respectively. The colonies of both strains were observed to be red pigmented on growth medium. Strain CBA1107(T) was observed to grow at 20-50 °C, in the presence of 15-30 % NaCl, at pH 6.0-9.0, and with 0.005-0.5 M Mg(2+). The cells of both strains lysed in distilled water. The DNA-DNA hybridization experiments showed that strain CBA1107(T) shared 97 % relatedness with CBA1108 and <50 % relatedness with H. pelagica JCM 16809(T), H. salina JCM 18549(T) and H. salifodinae JCM 18548(T). The genomic DNA G+C content of strain CBA1107(T) was determined to be 65.1 mol%. The major polar lipids of the two strains were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and glycolipids including sulfated mannosyl glucosyl diether and mannosyl glucosyl diether. Based on the polyphasic taxonomic analyses, the strains are considered to represent a new taxon for which the name Halolamina rubra sp. nov. is proposed, with the type strain CBA1107(T) (=CECT 8421(T) =JCM 19436(T)).
authors.affiliation: Jeju Center, Korea Basic Science Institute, Jeju, 690-140, Republic of Korea.
authors.forename: In-Tae
authors.initials: IT
authors.lastname: Cha
authors.forename: Kyung June
authors.initials: KJ
authors.lastname: Yim
authors.forename: Hye Seon
authors.initials: HS
authors.lastname: Song
authors.forename: Hae-Won
authors.initials: HW
authors.lastname: Lee
authors.forename: Dong-Wook
authors.initials: DW
authors.lastname: Hyun
authors.forename: Kil-Nam
authors.initials: KN
authors.lastname: Kim
authors.forename: Jong-Soon
authors.initials: JS
authors.lastname: Choi
authors.forename: Daekyung
authors.initials: D
authors.lastname: Kim
authors.forename: Sung-Jae
authors.initials: SJ
authors.lastname: Lee
authors.forename: Myung-Ji
authors.initials: MJ
authors.lastname: Seo
authors.forename: Hak-Jong
authors.initials: HJ
authors.lastname: Choi
authors.forename: Jin-Woo
authors.initials: JW
authors.lastname: Bae
authors.forename: Sung-Keun
authors.initials: SK
authors.lastname: Rhee
authors.forename: Seong Woon
authors.initials: SW
authors.lastname: Roh
authors.forename: Young-Do
authors.initials: YD
authors.lastname: Nam
mesh_terms: D001482:Base Composition; D016000:Cluster Analysis; D019641:DNA, Archaeal / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004783:Environmental Microbiology; D006017:Glycolipids / Q000032:analysis; D006216:Halobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012492:Salts / Q000378:metabolism*; D017422:Sequence Analysis, DNA
chemical_list: D019641:DNA, Archaeal; D004275:DNA, Ribosomal; D006017:Glycolipids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D012492:Salts