title: Developmental validation of a forensic rapid DNA-STR kit: Expressmarker 16.
abstract: DNA-STR analysis is widely used in the forensic science field and has important requirements on the analysis time to obtain faster inspections. The developed forensic STR kit, referred to as Expressmarker 16 (EX16), could shorten the amplification time to a minimum of 35min. It enables 16 STR loci to be co-detected, including 13 CODIS loci, D2S1338, D6S1043 and Amelogenin loci. The kit is validated by a series of tests formed by DNA mixtures, stutter ratios, optimized PCR protocols based on annealing temperature research, species specificities, inhibitors, sensitivity, and parallel tests according to FBI QAS (2009/2011) (QAS, 2009; SWGDAM, 2010). The results demonstrated that EX16 was a useful tool for rapid criminal investigation.
authors.affiliation: Institute of Forensic Science, Shanghai Municipal Public Security Bureau/Key Laboratory of Forensic Evidence and Scene Technology, Ministry of Public Security/Shanghai Key Laboratory of Crime Scene Evidence, Shanghai 200083, China. Electronic address: hgzhou803@hotmail.com.
authors.forename: Huaigu
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Institute of Forensic Science, Shanghai Municipal Public Security Bureau/Key Laboratory of Forensic Evidence and Scene Technology, Ministry of Public Security/Shanghai Key Laboratory of Crime Scene Evidence, Shanghai 200083, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Wu
authors.affiliation: Institute of Forensic Science, Shanghai Municipal Public Security Bureau/Key Laboratory of Forensic Evidence and Scene Technology, Ministry of Public Security/Shanghai Key Laboratory of Crime Scene Evidence, Shanghai 200083, China.
authors.forename: Ronghua
authors.initials: R
authors.lastname: Chen
authors.affiliation: Institute of Forensic Science, Shanghai Municipal Public Security Bureau/Key Laboratory of Forensic Evidence and Scene Technology, Ministry of Public Security/Shanghai Key Laboratory of Crime Scene Evidence, Shanghai 200083, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xu
authors.affiliation: AGCU ScienTech Incorporation, Wuxi 214174, China.
authors.forename: Zifang
authors.initials: Z
authors.lastname: Xia
authors.affiliation: AGCU ScienTech Incorporation, Wuxi 214174, China.
authors.forename: Yulin
authors.initials: Y
authors.lastname: Guo
authors.affiliation: AGCU ScienTech Incorporation, Wuxi 214174, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Zhang
authors.affiliation: AGCU ScienTech Incorporation, Wuxi 214174, China.
authors.forename: Weiguo
authors.initials: W
authors.lastname: Zheng
mesh_terms: D000818:Animals; D004247:DNA / Q000235:genetics*; D053803:Forensic Genetics; D005819:Genetic Markers; D006801:Humans; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction; D013045:Species Specificity
chemical_list: D005819:Genetic Markers; D004247:DNA
keywords: Forensic DNA analysis; Rapid identification; STR