title: Liposome encapsulated soy lecithin and cholesterol can efficiently replace chicken egg yolk in human semen cryopreservation medium.
abstract: Cryopreservation of spermatozoa plays a significant role in reproductive medicine and fertility preservation. Chicken egg yolk is used as an extender in cryopreservation of human spermatozoa using glycerol egg yolk citrate (GEYC) buffered medium. Even though 50% survival of spermatozoa is generally achieved with this method, the risk of high levels of endotoxins and transmission pathogens from chicken egg yolk is a matter of concern. In the present study we attempted to establish a chemically defined cryopreservation medium which can replace the chicken egg yolk without affecting sperm survival. Ejaculates from 28 men were cryopreserved with GEYC based freezing medium or liposome encapsulated soy lecithin-cholesterol based freezing medium (LFM). The semen samples were subjected to rapid thawing after 14 days of storage in liquid nitrogen. Post-thaw analysis indicated significantly higher post-thaw motility and sperm survival in spermatozoa cryopreserved with LFM compared to conventional GEYC freezing medium. The soy lecithin and cholesterol at the ratio of 80:20 with sucrose showed the highest percentage of post-thaw motility and survival compared to the other compositions. In conclusion, chemically defined cryopreservation medium with liposome encapsulated soy lecithin and cholesterol can effectively replace the chicken egg yolk from human semen cryopreservation medium without compromising post-thaw outcome.
authors.affiliation: Department of Pharmaceutics, Manipal College of Pharmaceutical Sciences .
authors.forename: Srinivas
authors.initials: S
authors.lastname: Mutalik
authors.forename: Sujith Raj
authors.initials: SR
authors.lastname: Salian
authors.forename: Kiran
authors.initials: K
authors.lastname: Avadhani
authors.forename: Jyothsna
authors.initials: J
authors.lastname: Menon
authors.forename: Haritima
authors.initials: H
authors.lastname: Joshi
authors.forename: Aswathi Raju
authors.initials: AR
authors.lastname: Hegde
authors.forename: Pratap
authors.initials: P
authors.lastname: Kumar
authors.forename: Guruprasad
authors.initials: G
authors.lastname: Kalthur
authors.forename: Satish Kumar
authors.initials: SK
authors.lastname: Adiga
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D002784:Cholesterol / Q000494:pharmacology*; D015925:Cryopreservation; D003451:Cryoprotective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D004530:Egg Yolk; D006801:Humans; D054709:Lecithins / Q000302:isolation & purification / Q000494:pharmacology*; D008081:Liposomes; D008297:Male; D012662:Semen Preservation / Q000379:methods*; D013025:Glycine max / Q000737:chemistry*; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects* / Q000473:pathology; D013997:Time Factors
chemical_list: D003451:Cryoprotective Agents; D054709:Lecithins; D008081:Liposomes; D002784:Cholesterol
keywords: Cholesterol; egg yolk; liposome; semen cryopreservation; soy lecithin; sperm motility