title: Development of a simple microarray for genotyping HIV-1 drug resistance mutations in the reverse transcriptase gene in rural Tanzania.
abstract: OBJECTIVE
  Aiming at a simple, inexpensive and robust tool for HIV-1 drug resistance genotyping during antiretroviral therapy (ART) we developed and validated a microarray-based detection of 25 drug resistance mutations most relevant for the Tanzanian ART regimen.
  
  
  METHODS
  A reverse transcriptase gene fragment was reverse-transcribed and amplified by reverse transcription-polymerase chain reaction (RT-PCR). Primers for mini-sequencing were designed based on alignments of the most prevalent local HIV-1 variants. Tagged primers were extended by fluorochrome-labelled dideoxynuclotide triphosphate (ddNTPs) to indicate the single-nucleotide polymorphism (SNP) allele of the sample tested, followed by hybridisation on treated microarray slides. Images were analysed with a laser scanner and genotype calling was performed using in-house developed software.
  
  
  RESULTS
  The microarray was validated with four cloned HIV-1 genome fragments from a Swiss HIV-1 cohort and 102 HIV-1 sequences amplified from the Tanzanian target population (field samples). Results were concordant with the Sanger sequencing SNP profile in 92.7% of 2550 SNP data points compared. Lack of signals in small number of SNPs was due to either failure in the extension reaction or hybridisation owing to mismatches between PCR product and extension primer.
  
  
  CONCLUSIONS
  Our study demonstrates the feasibility of hybridisation-based genotyping of drug resistance mutations of HIV, even though our microarray, which was designed for population studies, achieved only correct assignment of 92% of all SNPs in the tested samples.
authors.affiliation: Swiss Tropical and Public Health Institute, University of Basel, Switzerland.|University of Basel, Basel, Switzerland.|Muhimbili University of Health and Allied Sciences, Dar es Salaam, Tanzania.
authors.forename: Pax
authors.initials: P
authors.lastname: Masimba
authors.affiliation: Swiss Tropical and Public Health Institute, University of Basel, Switzerland.|University of Basel, Basel, Switzerland.
authors.forename: Janet
authors.initials: J
authors.lastname: Gare
authors.affiliation: University of Basel, Basel, Switzerland.|Department Biomedicine, University of Basel, Switzerland.
authors.forename: Thomas
authors.initials: T
authors.lastname: Klimkait
authors.affiliation: Swiss Tropical and Public Health Institute, University of Basel, Switzerland.|University of Basel, Basel, Switzerland.
authors.forename: Marcel
authors.initials: M
authors.lastname: Tanner
authors.affiliation: Swiss Tropical and Public Health Institute, University of Basel, Switzerland.|University of Basel, Basel, Switzerland.
authors.forename: Ingrid
authors.initials: I
authors.lastname: Felger
keywords: Genotipaje; HIV-1; Microarrays; VIH-1; drug resistance mutations; genotyping; génotypage; microarray; mutación de resistencia a medicamentos; mutations de résistance aux médicaments; reverse transcriptase; transcriptasa reversa; transcriptase inverse