title: Differential miRNA expression defines migration and reduced apoptosis in follicular thyroid carcinomas.
abstract: The objective of the study was to identify microRNAs (miRs) characteristic for follicular thyroid carcinoma (FTC) and to define their role in tumorigenesis. A miR-microarray study was conducted to identify miRs differentially expressed between FTCs and their surrounding tissues. Selection was further reinforced by a literature review. Four miRs were selected and confirmed by RT-qPCR: miR-146b, -183, -221 were up-regulated, whereas miR-199b down-regulated in FTCs. The influence of these miRs on cell proliferation, cell cycle, apoptosis and migration was studied in HTori and FTC-133 cells. Functional characterization suggests an impact of miR-183 and miR-146b in FTC development. Overexpression of both miRs significantly induces migration. Moreover, overexpression of miR-183 significantly represses apoptosis. MiR-199b and -221 do not have significant effects on proliferation, cell cycle, apoptosis or migration in HTori and FTC-133 cells. Our data suggest that miR-146b and miR-183 may influence FTC development through the induction of migration and apoptosis inhibition.
authors.affiliation: Department of Nuclear Medicine and Endocrine Oncology, M. Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice Branch, Wybrzeze AK 15, 44-101 Gliwice, Poland. Electronic address: bwojtas@io.gliwice.pl.
authors.forename: Bartosz
authors.initials: B
authors.lastname: Wojtas
authors.affiliation: Division of Endocrinology and Nephrology, University of Leipzig, Liebigstr. 21, D-04103 Leipzig, Germany. Electronic address: carolina.ferraz.endocrinologia@gmail.com.
authors.forename: Carolina
authors.initials: C
authors.lastname: Ferraz
authors.affiliation: Department of Nuclear Medicine and Endocrine Oncology, M. Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice Branch, Wybrzeze AK 15, 44-101 Gliwice, Poland; Systems Engineering Group, Institute of Automatic Control, Silesian University of Technology, Gliwice, Poland. Electronic address: tomasz.stokowy@polsl.pl.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Stokowy
authors.affiliation: Department of Pathology, University of Halle-Wittenberg, Halle (Saale), Germany. Electronic address: steffen.hauptmann@gmx.de.
authors.forename: Steffen
authors.initials: S
authors.lastname: Hauptmann
authors.affiliation: Tumor Pathology Department, M. Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice Branch, Wybrzeze AK 15, Gliwice, Poland. Electronic address: dlange@io.gliwice.pl.
authors.forename: Dariusz
authors.initials: D
authors.lastname: Lange
authors.affiliation: Department of General, Visceral and Vascular Surgery, University of Halle-Wittenberg, Halle (Saale), Germany. Electronic address: henning.dralle@medizin.uni-halle.de.
authors.forename: Henning
authors.initials: H
authors.lastname: Dralle
authors.affiliation: Department of General, Visceral, and Transplantation Surgery, University Medical Center of the Johannes Gutenberg University, Mainz, Germany. Electronic address: musholt@uni-mainz.de.
authors.forename: Thomas
authors.initials: T
authors.lastname: Musholt
authors.affiliation: Department of Nuclear Medicine and Endocrine Oncology, M. Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice Branch, Wybrzeze AK 15, 44-101 Gliwice, Poland. Electronic address: bjarzab@io.gliwice.pl.
authors.forename: Barbara
authors.initials: B
authors.lastname: Jarzab
authors.affiliation: Division of Endocrinology and Nephrology, University of Leipzig, Liebigstr. 21, D-04103 Leipzig, Germany. Electronic address: Ralf.Paschke@medizin.uni-leipzig.de.
authors.forename: Ralf
authors.initials: R
authors.lastname: Paschke
authors.affiliation: Division of Endocrinology and Nephrology, University of Leipzig, Liebigstr. 21, D-04103 Leipzig, Germany. Electronic address: Markus.Eszlinger@medizin.uni-leipzig.de.
authors.forename: Markus
authors.initials: M
authors.lastname: Eszlinger
mesh_terms: D018263:Adenocarcinoma, Follicular / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D017209:Apoptosis; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D013964:Thyroid Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology
chemical_list: C542542:MIRN146 microRNA, human; C533449:MIRN183 microRNA, human; D035683:MicroRNAs
keywords: Apoptosis; Follicular thyroid cancer; Migration; miRNA