title: The effect of nitidine chloride on the proliferation and apoptosis of nasopharyngeal carcinoma cells.
abstract: OBJECTIVE
  This study was conducted to investigate the effect of nitidine chloride (NC) on the proliferation and apoptosis of nasopharyngeal carcinoma cell line CNE1, CNE2, TWO3, and C666-1, and to explore its antitumor mechanism.
  
  
  METHODS
  NC was dissolved in IMDM medium and cultured with nasopharyngeal carcinoma cell line CNE1, CNE2, TWO3 and C666-1. Cell morphology, cell proliferation, cell apoptosis, p53 mRNA and p53 protein levels were assessed.
  
  
  RESULTS
  After incubation with NC for 24 h, typical apoptotic morphology was observed. NC inhibited the proliferation and induced apoptosis of all 4 cell lines in a time-dose dependent manner. p53 mRNA and p53 protein levels were significantly increased.
  
  
  CONCLUSIONS
  NC inhibited proliferation and induced apoptosis of nasopharyngeal carcinoma cells with upregulation of p53 gene.
authors.affiliation: Department of Radiotherapy, First Hospital of Guangxi Medical University, Guangxi, P.R. China.
authors.forename: Min
authors.initials: M
authors.lastname: Kang
authors.forename: Hesheng
authors.initials: H
authors.lastname: Ou
authors.forename: Rensheng
authors.initials: R
authors.lastname: Wang
authors.forename: Wenqi
authors.initials: W
authors.lastname: Liu
authors.forename: Anzhou
authors.initials: A
authors.lastname: Tang
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D053119:Benzophenanthridines / Q000494:pharmacology*; D002277:Carcinoma; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000188:drug therapy* / Q000473:pathology; D012333:RNA, Messenger / Q000096:biosynthesis*; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis*
chemical_list: D053119:Benzophenanthridines; D012333:RNA, Messenger; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; C013615:nitidine