title: Phosphorescent proteins for bio-imaging and site selective bio-conjugation of peptides and proteins with luminescent cyclometalated iridium(III) complexes.
abstract: A new bio-conjugation reaction for site selective modification of proteins and peptides with phosphorescent iridium(III) complexes has been developed; the Ir(III)-modified proteins and peptides display long emission lifetimes and large Stoke shifts that can be used for bio-imaging studies.
authors.affiliation: State Key Laboratory of Synthetic Chemistry, Institute of Molecular Functional Materials and Department of Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong, China. cmche@hku.hk.
authors.forename: Hoi-Yan
authors.initials: HY
authors.lastname: Shiu
authors.forename: Hiu-Chi
authors.initials: HC
authors.lastname: Chong
authors.forename: Yun-Chung
authors.initials: YC
authors.lastname: Leung
authors.forename: Taotao
authors.initials: T
authors.lastname: Zou
authors.forename: Chi-Ming
authors.initials: CM
authors.lastname: Che
mesh_terms: D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D056831:Coordination Complexes / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D003545:Cysteine / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D007495:Iridium / Q000737:chemistry*; D049408:Luminescent Agents / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D008163:Luminescent Measurements; D008856:Microscopy, Fluorescence; D010455:Peptides / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D011145:Polyvinyls / Q000737:chemistry; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D056831:Coordination Complexes; D049408:Luminescent Agents; D010455:Peptides; D011145:Polyvinyls; D011506:Proteins; D007495:Iridium; D003545:Cysteine