title: Lactobacillus pentosus var. plantarum C29 ameliorates memory impairment and inflammaging in a D-galactose-induced accelerated aging mouse model.
abstract: Aging is associated with Alzheimer's disease (AD), cardiovascular disease and cancer. Oxidative stress is considered as a major factor that accelerates the aging process. To understand the ability of lactic acid bacteria to ameliorate memory impairment caused by aging, we investigated the effect of Lactobacillus pentosus var. plantarum (C29), which is known to protect against scopolamine-induced memory impairment, on oxidative stress (D-galactose)-induced memory impairment in mice. D-Galactose was subcutaneously injected to 20-week old male C57BL/6J mice for 10 weeks, with oral administration of C29 for the final 5 weeks. Excessive intake of D-galactose not only impaired memory, which was indicated by passive avoidance, Y-maze, and Morris water-maze tasks, but also reduced the expression of brain-derived neurotrophic factor (BDNF) and hippocampal doublecortin (DCX) and the activation of cAMP response element-binding protein (CREB). C29 treatment ameliorated D-galactose-induced memory impairment and reversed the suppression of BDNF and DCX expression and CREB activation. Moreover, C29 decreased the expression of a senescence marker p16 and inflammation markers p-p65, p-FOXO3a, cyclooxygenase (COX)-2, and inducible NO synthase (iNOS). C29 treatment inhibited D-galactose-induced expression of M1 polarization markers tumor necrosis factor-α and arginase II, and attenuated the d-galactose-suppressed expression of M2 markers IL-10, arginase I and CD206. Taken together, these findings suggest that C29 may ameliorate memory impairment and M1 macrophage-polarized inflammation caused by aging.
authors.affiliation: Department of Pharmacy, College of Pharmacy, Kyung Hee University, Seoul 130-701, Republic of Korea.
authors.forename: Jae-Yeon
authors.initials: JY
authors.lastname: Woo
authors.affiliation: Department of Pharmacy, College of Pharmacy, Kyung Hee University, Seoul 130-701, Republic of Korea.
authors.forename: Wan
authors.initials: W
authors.lastname: Gu
authors.affiliation: Department of Pharmacy, College of Pharmacy, Kyung Hee University, Seoul 130-701, Republic of Korea.
authors.forename: Kyung-Ah
authors.initials: KA
authors.lastname: Kim
authors.affiliation: Department of Pharmacy, College of Pharmacy, Kyung Hee University, Seoul 130-701, Republic of Korea; Department of Food and Nutrition, Kyung Hee University, Seoul 130-701, Republic of Korea.
authors.forename: Se-Eun
authors.initials: SE
authors.lastname: Jang
authors.affiliation: Department of Food and Nutrition, Kyung Hee University, Seoul 130-701, Republic of Korea.
authors.forename: Myung Joo
authors.initials: MJ
authors.lastname: Han
authors.affiliation: Department of Pharmacy, College of Pharmacy, Kyung Hee University, Seoul 130-701, Republic of Korea. Electronic address: dhkim@khu.ac.kr.
authors.forename: Dong-Hyun
authors.initials: DH
authors.lastname: Kim
mesh_terms: D000375:Aging / Q000502:physiology*; D000818:Animals; D015415:Biomarkers / Q000032:analysis; D000089662:Doublecortin Protein; D005690:Galactose / Q000633:toxicity*; D007249:Inflammation / Q000628:therapy*; D007778:Lactobacillus / Q000254:growth & development*; D008297:Male; D008569:Memory Disorders / Q000628:therapy*; D008810:Mice, Inbred C57BL; D023421:Models, Animal; D018384:Oxidative Stress; D019936:Probiotics / Q000008:administration & dosage*
chemical_list: D015415:Biomarkers; C000714847:Dcx protein, mouse; D000089662:Doublecortin Protein; D005690:Galactose
keywords: Inflammaging; Lactobacillus pentosus var. plantarum C29; Memory; d-Galactose