title: Application of high-speed counter-current chromatography combined with macroporous resin for rapid enrichment and separation of three anthraquinone glycosides and one stilbene glycoside from Rheum tanguticum.
abstract: In this paper, an efficient method was successfully established by the combination of macroporous resin (MR) and high-speed counter-current chromatography (HSCCC) for rapid enrichment and separation of aloe-emodin 8-O-β-D-glucoside, emodin 1-O-β-D-glucoside, emodin 8-O-β-D-glucoside and piceatannol 4'-O-β-D-(6″-O-gallate)-glucoside. Six kinds of macroporous resins were investigated in the first step and X-5 macroporous resin was selected for the enrichment of the target compounds. The recoveries of the target compounds reached 89.0, 85.9, 82.3 and 84.9% respectively after 40% ethanol elution. In the second step, the target compounds were separated by HSCCC with a two-phase solvent system composed of chloroform/ethyl acetate/methanol/water (8:1:6:5, v/v). The established method will be helpful for further characterization and utilization of Rheum tanguticum. The results demonstrate that MR coupled with HSCCC is a powerful technique for separation of bioactive compounds from natural products.
authors.affiliation: Key Laboratory of Tibetan medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, P.R. China.
authors.forename: Tao
authors.initials: T
authors.lastname: Chen
authors.affiliation: Key Laboratory of Tibetan medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, P.R. China; University of the Chinese Academy of Sciences, Beijing 100049, P.R. China.
authors.forename: Yong-Ling
authors.initials: YL
authors.lastname: Liu
authors.affiliation: Key Laboratory of Tibetan medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, P.R. China.
authors.forename: Chen
authors.initials: C
authors.lastname: Chen
authors.affiliation: Key Laboratory of Tibetan medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, P.R. China; University of the Chinese Academy of Sciences, Beijing 100049, P.R. China.
authors.forename: Deng-Lang
authors.initials: DL
authors.lastname: Zou
authors.affiliation: Key Laboratory of Tibetan medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, P.R. China.
authors.forename: Jin-Mao
authors.initials: JM
authors.lastname: You
authors.affiliation: Key Laboratory of Tibetan medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, P.R. China. Electronic address: sunj@nwipb.cas.cn.
authors.forename: Jing
authors.initials: J
authors.lastname: Sun
authors.affiliation: Key Laboratory of Tibetan medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, P.R. China. Electronic address: liyulin@nwipb.cas.cn.
authors.forename: Yu-Lin
authors.initials: YL
authors.lastname: Li
mesh_terms: D000880:Anthraquinones / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D003377:Countercurrent Distribution / Q000379:methods*; D006027:Glycosides / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D019995:Laboratory Chemicals; D010936:Plant Extracts / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D016062:Porosity; D012250:Rheum / Q000737:chemistry*; D013267:Stilbenes / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D000880:Anthraquinones; D006027:Glycosides; D019995:Laboratory Chemicals; D010936:Plant Extracts; D013267:Stilbenes
keywords: Anthraquinone Glycoside; High-speed counter-current chromatography; Macroporous resin; Rheum tanguticum; Stilbene glycoside