title: HPLC determination of brain biogenic amines following treatment with bispyridinium aldoxime K203.
abstract: Effect of a new acetylcholine-esterase reactivator, K203 as a new potential antidote in organophosphate intoxications was studied on dopamine (DA), homovanillic acid (HVA), serotonin (5-HT) and 5-hydroxyindolacetic acid (5-HIAA) levels in seven brain regions (cerebellum, spinal cord, hippocampus, hypothalamus, striatum, medulla oblongata and frontal cortex) of rats by an optimized and validated HPLC method. No significant change in brain level of these neurotransmitters was found either 15 or 60 min following treatment. However, when 5-HIAA/5-HT ratios were calculated as measure of turnover, significant decreases were found in the cerebellum, hippocampus, hypothalamus and the frontal cortex 15 min following K203 administration, but after 60 min only in the frontal cortex.
authors.affiliation: Semmelweis University Department of Pharmacodynamics Nagyvárad tér 4 H-1089 Budapest Hungary.
authors.forename: F
authors.initials: F
authors.lastname: Hashemi
authors.affiliation: Semmelweis University Department of Pharmacology and Pharmacotherapy Budapest Hungary.
authors.forename: R
authors.initials: R
authors.lastname: Laufer
authors.affiliation: Semmelweis University Department of Pharmacodynamics Nagyvárad tér 4 H-1089 Budapest Hungary.
authors.forename: P
authors.initials: P
authors.lastname: Szegi
authors.affiliation: Semmelweis University Department of Pharmacodynamics Nagyvárad tér 4 H-1089 Budapest Hungary.
authors.forename: V
authors.initials: V
authors.lastname: Csomor
authors.affiliation: Semmelweis University Department of Pharmacology and Pharmacotherapy Budapest Hungary.
authors.forename: H
authors.initials: H
authors.lastname: Kalász
authors.affiliation: Semmelweis University Department of Pharmacodynamics Nagyvárad tér 4 H-1089 Budapest Hungary.
authors.forename: Kornélia
authors.initials: K
authors.lastname: Tekes
mesh_terms: D000818:Animals; D000931:Antidotes / Q000494:pharmacology*; D001679:Biogenic Amines / Q000378:metabolism*; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004298:Dopamine / Q000378:metabolism; D006719:Homovanillic Acid / Q000378:metabolism; D006897:Hydroxyindoleacetic Acid / Q000378:metabolism; D008297:Male; D010091:Oximes / Q000494:pharmacology*; D011726:Pyridinium Compounds / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012701:Serotonin / Q000378:metabolism; D013997:Time Factors
chemical_list: C524716:1-(4-carbamoylpyridinium)-4-(4-hydroxyiminomethylpyridinium)but-2-ene; D000931:Antidotes; D001679:Biogenic Amines; D010091:Oximes; D011726:Pyridinium Compounds; D012701:Serotonin; D006897:Hydroxyindoleacetic Acid; D004298:Dopamine; D006719:Homovanillic Acid
keywords: HPLC; K203; acetylcholine-esterase reactivator; bispyridinium aldoximes; dopamine; organophosphate antidotes; rat brain; serotonin