title: The role of EaIκB-α, an IκB-α homologue in Epinephelus akaara, involved in innate immune response.
abstract: The inhibitor κB (IκB) protein is an important component of the nuclear factor kB (NF-κB) signaling pathway. We have cloned and characterized the IκB-α homologue gene (named EaIκB-α) from grouper (Epinephelus akaara), expressed it in Escherichia coli and generated a rabbit polyclonal antibody. EaIκB-α encodes a 308-amino acid protein with evolutionarily and functionally homology to mammalian IκB-α. Over-expression of wild-type EaIκB-α or a mutated, nondegradable EaIκB-α (EaIκB-αM) in Escherichia coli both inhibited the degradation of cellular EaIκB-α, activation of NF-κB and expression of tumor necrosis factor α (TNF-α). EaIκB-α protein is restricted to the cytoplasm in unstimulated cell. Activated by diverse stimuli, EaIκB-α is degraded, thereby releasing NF-κB into nucleus and activating NF-κB-mediated transcripts. EaIκB-α and other studied NF-κB target genes are induced by LPS in kidney and liver, which suggests that EaIκB-α may play an important role in immune response of grouper.
authors.forename: Weijun
authors.initials: W
authors.lastname: Mai
authors.forename: Fang
authors.initials: F
authors.lastname: Huang
authors.forename: Peng
authors.initials: P
authors.lastname: Liu
mesh_terms: D000818:Animals; D001492:Bass / Q000235:genetics / Q000276:immunology*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D020672:I-kappa B Proteins / Q000235:genetics / Q000378:metabolism*; D007113:Immunity, Innate; D000072000:NF-KappaB Inhibitor alpha; D011817:Rabbits; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000276:immunology / Q000302:isolation & purification; D017386:Sequence Homology, Amino Acid
chemical_list: D020672:I-kappa B Proteins; D011994:Recombinant Proteins; D000072000:NF-KappaB Inhibitor alpha