title: Nerve cells culture from lumbar spinal cord on surfaces modified by plasma pyrrole polymerization.
abstract: Currently, there are several techniques for modified cell culture surfaces under research to improve cell growth and adhesion. Recently, different methods have been used for surface coating, using biomolecules that enhance cell attachment and growth of nerve cells from spinal cord, such as the use of Poly-DL-Ornithine/Laminin. Plasma-polymerized pyrrole (PPy)-treated surfaces have showed improvement on surfaces biocompatibility with the cells in culture since they do not interfere with any of the biological cell functions. In the present work, we present a novel mouse nerve cell culture technique, using PPy-treated cell culture surfaces. A comparative study of cell survival using Poly-DL-Ornithine/Laminin-treated surfaces was performed. Our results of cell survival when compared with data already reported by other investigators, show that cells cultured on the PPy-modified surface increased survival up to 21 days when compared with Poly-DL-Ornithine/Laminin-coated culture, where 8 days cell survival was obtained. There were electrical and morphological differences in the nerve cells grown in the different surfaces. By comparing the peak ion currents of Poly-DL-Ornithine/Laminin-seeded cells for 8 days with cells grown for 21 days on PPy, an increase of 516% in the Na(+) current and 127% in K(+) currents in cells seeded on PPy were observed. Immunofluorescence techniques showed the presence of cell synapses and culture viability after 21 days. Our results then showed that PPy-modified surfaces are an alternative culture method that increases nerve cells survival from lumbar spinal cord cell culture by preserving its electrical and morphological features.
authors.affiliation: a Department of Electrical Engineer , Division of Biomedical Engineer, Universidad Autonoma Metropolitana , Unidad Iztapalapa , A.P. 55-534 , Iztapalapa , México, D.F., Mexico.
authors.forename: E
authors.initials: E
authors.lastname: Zuñiga-Aguilar
authors.forename: R
authors.initials: R
authors.lastname: Olayo
authors.forename: O
authors.initials: O
authors.lastname: Ramírez-Fernández
authors.forename: J
authors.initials: J
authors.lastname: Morales
authors.forename: R
authors.initials: R
authors.lastname: Godínez
mesh_terms: D000200:Action Potentials; D000818:Animals; D018929:Cell Culture Techniques / Q000295:instrumentation*; D002470:Cell Survival; D002478:Cells, Cultured; D003470:Culture Media; D017097:Electric Impedance; D007477:Ions / Q000378:metabolism; D007797:Laminin; D008159:Lumbar Vertebrae; D008564:Membrane Potentials; D051379:Mice; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D058105:Polymerization; D011188:Potassium / Q000378:metabolism; D011758:Pyrroles / Q000737:chemistry*; D012964:Sodium / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared; D013116:Spinal Cord / Q000166:cytology*; D013499:Surface Properties; D013569:Synapses / Q000502:physiology; D013997:Time Factors
chemical_list: D003470:Culture Media; D007477:Ions; D007797:Laminin; D011758:Pyrroles; D012964:Sodium; D011188:Potassium