title: Expression, purification, crystallization and preliminary X-ray diffraction analysis of Aspergillus terreus endo-β-1,4-glucanase from glycoside hydrolase family 12.
abstract: Endoglucanases are important enzymes that are involved in the modification and degradation of cellulose. Filamentous fungi such as Aspergillus terreus are effective biomass degraders in nature owing to their capacity to produce an enzymatic arsenal of glycoside hydrolases, including endoglucanase from glycoside hydrolase family 12 (GH12). The A. terreus GH12 endoglucanase was cloned and overexpressed in A. nidulans, purified and crystallized. A single crystal was obtained from a solution consisting of 2 M ammonium sulfate, 5%(v/v) 2-propanol. X-ray diffraction data were collected to a resolution of 1.85 Å using synchrotron radiation and a preliminary molecular-replacement solution was obtained in the trigonal space group P3(2)21. The unit-cell parameters were a = b = 103.24, c = 48.96 Å.
authors.affiliation: Instituto de Física de São Carlos, Universidade de São Paulo, Avenida Trabalhador Saocarlense 400, 13566-590 São Carlos-SP, Brazil.
authors.forename: Fernando
authors.initials: F
authors.lastname: Segato
authors.affiliation: Instituto de Física de São Carlos, Universidade de São Paulo, Avenida Trabalhador Saocarlense 400, 13566-590 São Carlos-SP, Brazil.
authors.forename: Gabriela L
authors.initials: GL
authors.lastname: Berto
authors.affiliation: Instituto de Física de São Carlos, Universidade de São Paulo, Avenida Trabalhador Saocarlense 400, 13566-590 São Carlos-SP, Brazil.
authors.forename: Evandro
authors.initials: E
authors.lastname: Ares de Araújo
authors.affiliation: Instituto de Física de São Carlos, Universidade de São Paulo, Avenida Trabalhador Saocarlense 400, 13566-590 São Carlos-SP, Brazil.
authors.forename: João Renato
authors.initials: JR
authors.lastname: Muniz
authors.affiliation: Instituto de Física de São Carlos, Universidade de São Paulo, Avenida Trabalhador Saocarlense 400, 13566-590 São Carlos-SP, Brazil.
authors.forename: Igor
authors.initials: I
authors.lastname: Polikarpov
mesh_terms: D000595:Amino Acid Sequence; D001230:Aspergillus / Q000201:enzymology*; D001483:Base Sequence; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods*; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D006026:Glycoside Hydrolases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011487:Protein Conformation; D017386:Sequence Homology, Amino Acid
chemical_list: D017931:DNA Primers; D006026:Glycoside Hydrolases
keywords: Aspergillus terreus; endo-β-1,4-glucanase