title: Biocompatible and photostable AIE dots with red emission for in vivo two-photon bioimaging.
abstract: Bioimaging systems with cytocompatibility, photostability, red fluorescence, and optical nonlinearity are in great demand. Herein we report such a bioimaging system. Integration of tetraphenylethene (T), triphenylamine (T), and fumaronitrile (F) units yielded adduct TTF with aggregation-induced emission (AIE). Nanodots of the AIE fluorogen with efficient red emission were fabricated by encapsulating TTF with phospholipid. The AIE dots enabled three-dimensional dynamic imaging with high resolution in blood vessels of mouse brain under two-photon excitation.
authors.affiliation: 1] Centre for Optical and Electromagnetic Research, Zhejiang Provincial Key Laboratory for Sensing Technologies, Joint Research Center of Photonics of the Royal Institute of Technology and Zhejiang University (JORCEP), Zhejiang University, Hangzhou 310058, China [2] State Key Laboratory of Organic-Inorganic Composites, Beijing University of Chemical Technology, Beijing 100029, China [3].
authors.forename: Dan
authors.initials: D
authors.lastname: Wang
authors.affiliation: 1] Centre for Optical and Electromagnetic Research, Zhejiang Provincial Key Laboratory for Sensing Technologies, Joint Research Center of Photonics of the Royal Institute of Technology and Zhejiang University (JORCEP), Zhejiang University, Hangzhou 310058, China [2].
authors.forename: Jun
authors.initials: J
authors.lastname: Qian
authors.affiliation: Department of Chemistry, Division of Biomedical Engineering, and Institute for Advanced Study, The Hong Kong University of Science and Technology (HKUST), Clear Water Bay, Kowloon, Hong Kong, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Qin
authors.affiliation: MoE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
authors.forename: Anjun
authors.initials: A
authors.lastname: Qin
authors.affiliation: 1] Department of Chemistry, Division of Biomedical Engineering, and Institute for Advanced Study, The Hong Kong University of Science and Technology (HKUST), Clear Water Bay, Kowloon, Hong Kong, China [2] Guangdong Innovative Research Team, SCUT-HKUST Joint Research Laboratory, State Key Laboratory of Luminescent Materials and Devices, South China University of Technology (SCUT), Guangzhou 510640, China.
authors.forename: Ben Zhong
authors.initials: BZ
authors.lastname: Tang
authors.affiliation: Centre for Optical and Electromagnetic Research, Zhejiang Provincial Key Laboratory for Sensing Technologies, Joint Research Center of Photonics of the Royal Institute of Technology and Zhejiang University (JORCEP), Zhejiang University, Hangzhou 310058, China.
authors.forename: Sailing
authors.initials: S
authors.lastname: He
mesh_terms: D000588:Amines / Q000737:chemistry; D000818:Animals; D001808:Blood Vessels / Q000187:drug effects / Q000648:ultrastructure*; D001921:Brain / Q000098:blood supply / Q000187:drug effects / Q000648:ultrastructure*; D003952:Diagnostic Imaging / Q000379:methods*; D004337:Drug Carriers / Q000737:chemistry; D004339:Drug Compounding; D005456:Fluorescent Dyes; D005650:Fumarates / Q000737:chemistry; D051379:Mice; D036641:Microscopy, Fluorescence, Multiphoton; D017785:Photons; D011095:Polyethylenes / Q000737:chemistry; D045663:Quantum Dots / Q000737:chemistry; D013730:Terphenyl Compounds / Q000737:chemistry
chemical_list: D000588:Amines; D004337:Drug Carriers; D005456:Fluorescent Dyes; D005650:Fumarates; D011095:Polyethylenes; D013730:Terphenyl Compounds; C057444:fumaronitrile