title: Dynamic cell culture on porous biopolymer microcarriers in a spinner flask for bone tissue engineering: a feasibility study.
abstract: Porous microspherical carriers have great promise for cell culture and tissue engineering. Dynamic cultures enable more uniform cell population and effective differentiation than static cultures. Here we applied dynamic spinner flask culture for the loading and multiplication of cells onto porous biopolymer microcarriers. The abilities of the microcarriers to populate cells and to induce osteogenic differentiation were examined and the feasibility of in vivo delivery of the constructs was addressed. Over time, the porous microcarriers enabled cell adhesion and expansion under proper dynamic culture conditions. Osteogenic markers were substantially expressed by the dynamic cell cultures. The cell-cultured microcarriers implanted in the mouse subcutaneous tissue for 4 weeks showed excellent tissue compatibility, with minimal inflammatory signs and significant induction of bone tissues. This first report on dynamic culture of porous biopolymer microcarriers providing an effective tool for bone tissue engineering.
authors.affiliation: Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan, 330-714, Republic of Korea, gzhjin2008@dankook.ac.kr.
authors.forename: Guang-Zhen
authors.initials: GZ
authors.lastname: Jin
authors.forename: Jeong-Hui
authors.initials: JH
authors.lastname: Park
authors.forename: Seog-Jin
authors.initials: SJ
authors.lastname: Seo
authors.forename: Hae-Won
authors.initials: HW
authors.lastname: Kim
mesh_terms: D000818:Animals; D001704:Biopolymers; D001842:Bone and Bones; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D005240:Feasibility Studies; D051379:Mice; D008863:Microspheres; D013234:Stem Cells / Q000502:physiology*; D023822:Tissue Engineering
chemical_list: D001704:Biopolymers