title: Measuring microtubule growth and gliding in Caenorhabditis elegans embryos.
abstract: Microtubule plus-tip tracking is a powerful method to measure microtubule growth dynamics in vivo. Here we outline an approach that exploits live confocal microscopy of a GFP-tagged EB1-like protein to measure microtubule growth behavior and minus-end-directed microtubule motor activity at the cortex of Caenorhabditis elegans embryos. The EB1 velocity assay (EVA) provides a method to reproducibly monitor motor- and non-motor-assisted microtubule movements.
authors.affiliation: Department of Biological Sciences, University of Alberta, CW405, Biological Sciences Building, Edmonton, Canada, AB T6G 2E9.
authors.forename: Justus
authors.initials: J
authors.lastname: Tegha-Dunghu
authors.forename: Eva M
authors.initials: EM
authors.lastname: Gusnowski
authors.forename: Martin
authors.initials: M
authors.lastname: Srayko
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000378:metabolism*; D004398:Dyneins / Q000378:metabolism; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000378:metabolism*; D015870:Gene Expression; D017930:Genes, Reporter; D007091:Image Processing, Computer-Assisted; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D008870:Microtubules / Q000378:metabolism*
chemical_list: D008869:Microtubule-Associated Proteins; D004398:Dyneins