title: Bimodal imprint chips for peptide screening: integration of high-throughput sequencing by MS and affinity analyses by surface plasmon resonance imaging.
abstract: Peptide probes and drugs have widespread applications in disease diagnostics and therapy. The demand for peptides ligands with high affinity and high specificity toward various targets has surged in the biomedical field in recent years. The traditional peptide screening procedure involves selection, sequencing, and characterization steps, and each step is manual and tedious. Herein, we developed a bimodal imprint microarray system to embrace the whole peptide screening process. Silver-sputtered silicon chip fabricated with microwell array can trap and pattern the candidate peptide beads in a one-well-one-bead manner. Peptides on beads were photocleaved in situ. A portion of the peptide in each well was transferred to a gold-coated chip to print the peptide array for high-throughput affinity analyses by surface plasmon resonance imaging (SPRi), and the peptide left in the silver-sputtered chip was ready for in situ single bead sequencing by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Using the bimodal imprint chip system, affinity peptides toward AHA were efficiently screened out from the 7 × 10(4) peptide library. The method provides a solution for high efficiency peptide screening.
authors.affiliation: CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety, National Center for Nanoscience and Technology of China , Beijing 100190, China.
authors.forename: Weizhi
authors.initials: W
authors.lastname: Wang
authors.forename: Menglin
authors.initials: M
authors.lastname: Li
authors.forename: Zewen
authors.initials: Z
authors.lastname: Wei
authors.forename: Zihua
authors.initials: Z
authors.lastname: Wang
authors.forename: Xiangli
authors.initials: X
authors.lastname: Bu
authors.forename: Wenjia
authors.initials: W
authors.lastname: Lai
authors.forename: Shu
authors.initials: S
authors.lastname: Yang
authors.forename: He
authors.initials: H
authors.lastname: Gong
authors.forename: Hui
authors.initials: H
authors.lastname: Zheng
authors.forename: Yuqiao
authors.initials: Y
authors.lastname: Wang
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.forename: Qin
authors.initials: Q
authors.lastname: Li
authors.forename: Qiaojun
authors.initials: Q
authors.lastname: Fang
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Hu
mesh_terms: D006046:Gold; D059014:High-Throughput Nucleotide Sequencing / Q000295:instrumentation* / Q000379:methods*; D056656:Lab-On-A-Chip Devices; D013058:Mass Spectrometry / Q000379:methods*; D010455:Peptides / Q000737:chemistry*; D012825:Silicon; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D010455:Peptides; D006046:Gold; D012825:Silicon