title: Gold nanoparticle-based immuno-PCR for detection of tau protein in cerebrospinal fluid.
abstract: Tau protein in cerebrospinal fluid (CSF) is an important biomarker of Alzheimer's disease and some other brain diseases. Enzyme-linked immunosorbent assays (ELISAs) have been mostly used for quantification of tau and other biomarkers in CSF. However, these assays do not have sufficient sensitivity and dynamic range. In this study we tested the suitability of gold nanoparticles functionalized with tau-specific monoclonal antibody and oligonucleotide template for immuno-polymerase chain reaction (Nano-iPCR) quantification of tau protein in human CSF samples and compared it with ELISA, either commercial or newly developed with tyramide signal amplification. Our data indicate that Nano-iPCR is superior in sensitivity and detection range to ELISA in tau protein detection.
authors.affiliation: Laboratory of Signal Transduction, Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, CZ-142 20, Prague 4, Czech Republic.
authors.forename: Lucie
authors.initials: L
authors.lastname: Stegurová
authors.affiliation: Laboratory of Biology of Cytoskeleton, Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, CZ-142 20, Prague 4, Czech Republic.
authors.forename: Eduarda
authors.initials: E
authors.lastname: Dráberová
authors.affiliation: Laboratory of Biochemistry and Brain Pathophysiology, Prague Psychiatric Center, National Institute of Mental Health, CZ-181 03, Prague 8, Czech Republic; AD Center, Prague Psychiatric Center, National Institute of Mental Health, CZ-181 03, Prague 8, Czech Republic; Department of Neurology, Third Faculty of Medicine, Charles University in Prague, University Hospital Královské Vinohrady, CZ-100 34, Prague 10, Czech Republic.
authors.forename: Ales
authors.initials: A
authors.lastname: Bartos
authors.affiliation: Laboratory of Biology of Cytoskeleton, Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, CZ-142 20, Prague 4, Czech Republic.
authors.forename: Pavel
authors.initials: P
authors.lastname: Dráber
authors.affiliation: Laboratory of Biochemistry and Brain Pathophysiology, Prague Psychiatric Center, National Institute of Mental Health, CZ-181 03, Prague 8, Czech Republic; AD Center, Prague Psychiatric Center, National Institute of Mental Health, CZ-181 03, Prague 8, Czech Republic.
authors.forename: Daniela
authors.initials: D
authors.lastname: Rípová
authors.affiliation: Laboratory of Signal Transduction, Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, CZ-142 20, Prague 4, Czech Republic. Electronic address: draberpe@img.cas.cz.
authors.forename: Petr
authors.initials: P
authors.lastname: Dráber
mesh_terms: D000544:Alzheimer Disease / Q000175:diagnosis*; D000911:Antibodies, Monoclonal / Q000276:immunology; D015415:Biomarkers / Q000134:cerebrospinal fluid; D004797:Enzyme-Linked Immunosorbent Assay; D006046:Gold; D006801:Humans; D053768:Metal Nanoparticles; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014439:Tyramine / Q000378:metabolism; D016875:tau Proteins / Q000134:cerebrospinal fluid*
chemical_list: D000911:Antibodies, Monoclonal; D015415:Biomarkers; C054369:MAPT protein, human; D016875:tau Proteins; D006046:Gold; D014439:Tyramine
keywords: ELISA; Gold nanoparticles; PCR; Tau protein