title: EIF5A2 is a novel chemoresistance gene in breast cancer.
abstract: BACKGROUND
  The eIF5A2 gene (encoding the eukaryotic initiation factor 5A2) located at 3q26 is a putative oncogene that is overexpressed in colon and rectal carcinomas, lung cancer and hepatocellular carcinoma. EIF5A2 overexpression correlates significantly with tumor metastasis and is an adverse prognostic marker. However, eIF-5A2 overexpression in breast cancer and its effect on chemotherapy are unknown.
  
  
  METHODS
  We measured eIF-5A2 expression and doxorubicin sensitivity in different human breast cancer cell lines (Bcap-1937, HCC1937, and MCF-7). To investigate a role for eIF-5A2 in chemoresistance, cells were treated with eIF-5A2-siRNA, exposed to various concentrations of doxorubicin, and toxicity was assayed by CCK-8 (cell counting kit).
  
  
  RESULTS
  The eIF-5A2 expression levels varied among breast cancer cells. Higher expression levels correlated with decreased doxorubicin sensitivity. Silencing of eIF-5A2 significantly improved doxorubicin toxicity in all three breast cancer cell lines.
  
  
  CONCLUSIONS
  This study shows that eIF-5A2 plays an important role in doxorubicin chemoresistance in breast cancer cells.
authors.affiliation: Breast Center of the First Affiliated Hospital, School of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou, 310003, Zhejiang, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Breast Center of the First Affiliated Hospital, School of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou, 310003, Zhejiang, China.
authors.forename: Feiya
authors.initials: F
authors.lastname: Du
authors.affiliation: Breast Center of the First Affiliated Hospital, School of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou, 310003, Zhejiang, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.affiliation: Breast Center of the First Affiliated Hospital, School of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou, 310003, Zhejiang, China.
authors.forename: Minya
authors.initials: M
authors.lastname: Yao
authors.affiliation: Breast Center of the First Affiliated Hospital, School of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou, 310003, Zhejiang, China.
authors.forename: Kezhen
authors.initials: K
authors.lastname: Lv
authors.affiliation: Breast Center of the First Affiliated Hospital, School of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou, 310003, Zhejiang, China. peifenfu@163.com.
authors.forename: Peifen
authors.initials: P
authors.lastname: Fu
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor / Q000187:drug effects; D004317:Doxorubicin / Q000494:pharmacology; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D055785:Gene Knockdown Techniques; D006801:Humans; D061986:MCF-7 Cells / Q000187:drug effects; D010448:Peptide Initiation Factors / Q000235:genetics* / Q000378:metabolism; D034741:RNA, Small Interfering; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D000097574:Eukaryotic Translation Initiation Factor 5A
chemical_list: D000903:Antibiotics, Antineoplastic; D004317:Doxorubicin; D010448:Peptide Initiation Factors; D034741:RNA, Small Interfering; D016601:RNA-Binding Proteins; D000097574:Eukaryotic Translation Initiation Factor 5A
keywords: Breast cancer; Chemoresistance; Doxorubicin; eIF-5A2