title: Detection of genetic variations in Orf virus isolates epidemic in Xinjiang China.
abstract: Orf is an exanthemous viral disease seriously threatening the goat and sheep industry and widely epidemic in the goat and sheep populations in Xinjiang, China. In order to investigate the genetic variability of the orf virus (ORFV), three virus isolates (SHZ1, SHZ2, and SHZ3) were isolated by PCR and Vero cell culture using the clinical samples from the lips of the lambs suspected of ORFV infection. The isolates were further verified by electron microscopy and animal infection experiments. The protective antigen genes B2L, F1L, and virulence genes VIR, GIF, and VEGF in the isolates were cloned, sequenced and analyzed for genetic evolution. The results showed that B2L and F1L were relatively conservative with homology 86.7-97.9%, while VIR, GIF, particularly VEGF were considerably variable with homology 71.5-97.9% at amino acid sequence level, respectively. Phylogenetic tree analysis based on B2L and VIR showed that the isolates SHZ1 and SHZ2 were closely related with the Taiwan isolates. This is the first report to confirm that there have been genetic variations in the Xinjiang ORFV isolates. The findings provide molecular evidence about the genetic variability of the major antigenic and virulence genes in the virus isolates epidemic in Xinjiang.
authors.affiliation: College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, China.
authors.forename: Haibo
authors.initials: H
authors.lastname: Yang
authors.forename: Qingling
authors.initials: Q
authors.lastname: Meng
authors.forename: Jun
authors.initials: J
authors.lastname: Qiao
authors.forename: Yelong
authors.initials: Y
authors.lastname: Peng
authors.forename: Kun
authors.initials: K
authors.lastname: Xie
authors.forename: Yucheng
authors.initials: Y
authors.lastname: Liu
authors.forename: Hailong
authors.initials: H
authors.lastname: Zhao
authors.forename: Xuepeng
authors.initials: X
authors.lastname: Cai
authors.forename: Chuangfu
authors.initials: C
authors.lastname: Chen
mesh_terms: D032761:Animal Experimentation; D000818:Animals; D002681:China; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D016000:Cluster Analysis; D004474:Ecthyma, Contagious / Q000821:virology*; D058872:Epidemics; D019143:Evolution, Molecular; D014644:Genetic Variation; D006041:Goats; D008046:Lip / Q000821:virology; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D009923:Orf virus / Q000145:classification / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification*; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D012756:Sheep; D014709:Vero Cells; D014764:Viral Proteins / Q000235:genetics; D014776:Virus Cultivation
chemical_list: D014764:Viral Proteins
keywords: Contagious ecthyma; Domestic ruminants; Genetic variability; Orf virus; Parapoxvirus; Sheep and goats; Zoonosis