title: [Determination of four biogenic amine metabolites in urine by high-performance liquid chromatography].
abstract: OBJECTIVE
  To establish a method for simultaneously determining vanilmandelic acid (VMA), 5-hydroxyindoleacetic (5-HIAA), 3, 4-dihydroxyphenylacetic acid (DOPAC), and homovanillic acid (HVA) in urine by high-performance liquid chromatography (HPLC).
  
  
  METHODS
  After being filtered with a 0.45 µm membrane syringe filter, the urinary samples were injected directly into the HPLC system using a C18 chromatographic column and a fluorescence detector. The excitation and emission wavelengths were chose as 280 nm and 315 nm, respectively, and the urinary samples were carried with a mobile phase of methanol-0.1 mol/L phosphate buffered solution (V/V = 20:80) at a flow rate of 1.0 ml/min and an injection volume of 20 µl.
  
  
  RESULTS
  Using the method reported here, the correlation coefficients of VMA, 5-HIAA, DOPAC, and HVA were 0.9999, 0.9998, 0.9997, 0.9999, respectively, over linear ranges of 0-2.5, 0-2.0, 0-2.0, and 0-2.5 µg/ml, the minimum detectable concentrations were 0.006, 0.008, 0.012, and 0.0082 µg/ml, the average precisions were 4.2%, 3.7%, 4.9%, and 3.6%, and the recovery rates were 91%∼102%, 93%∼101%, 94%∼101%, and 89%∼ 102%.
  
  
  CONCLUSIONS
  This determination method is simple, efficient, accurate, and sensitive for the simultaneous detection of VMA, 5-HIAA, DOPAC, and HVA in urine.
authors.affiliation: Henan Provincial Institute of Occupational Health, Zhengzhou 450052, China.
authors.forename: Kaiyou
authors.initials: K
authors.lastname: Jiang
authors.affiliation: Henan Provincial Institute of Occupational Health, Zhengzhou 450052, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wu
authors.affiliation: Henan Provincial Institute of Occupational Health, Zhengzhou 450052, China.
authors.forename: Wenhua
authors.initials: W
authors.lastname: Qin
authors.affiliation: Henan Provincial Institute of Occupational Health, Zhengzhou 450052, China.
authors.forename: Guizhen
authors.initials: G
authors.lastname: Gu
authors.affiliation: Henan Provincial Institute of Occupational Health, Zhengzhou 450052, China. E-mail: yu-shanfa@163.com.
authors.forename: Shanfa
authors.initials: S
authors.lastname: Yu
mesh_terms: D001679:Biogenic Amines / Q000652:urine*; D002851:Chromatography, High Pressure Liquid; D006719:Homovanillic Acid / Q000652:urine; D006801:Humans; D006897:Hydroxyindoleacetic Acid / Q000652:urine; D014642:Vanilmandelic Acid / Q000652:urine
chemical_list: D001679:Biogenic Amines; D006897:Hydroxyindoleacetic Acid; D014642:Vanilmandelic Acid; D006719:Homovanillic Acid