title: Diosgenin inhibits the migration of human breast cancer MDA-MB-231 cells by suppressing Vav2 activity.
abstract: Diosgenin, a naturally occurring steroidal saponin, possess tumor therapeutic potential. However, the effect of diosgenin on cancer metastasis remains poorly understood. In this study, we performed in vitro experiments to investigate the inhibitory activity of diosgenin on human breast cancer MDA-MB-231 cell migration, and reveal the possible mechanism. Diosgenin caused a marked inhibition of cell migration in MDA-MB-231 cell by transwell assay. In addition, diosgenin significantly impacted MDA-MB-231 cell migratory behavior under real-time observation. We also found diosgenin significantly inhibited actin polymerization, Vav2 phosphorylation and Cdc42 activation, which might be, at least in part, attributed to the anti-metastatic potential of diosgenin. These findings reveal a new therapeutic potential of diosgenin for human breast cancer metastasis therapy.
authors.affiliation: College of Chinese Medicine Materials, Jilin Agricultural University, Changchun 130118, China.
authors.forename: Zhongmei
authors.initials: Z
authors.lastname: He
authors.affiliation: College of Chinese Medicine Materials, Jilin Agricultural University, Changchun 130118, China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Chen
authors.affiliation: The First Affiliated Hospital of Changchun University of Traditional Medicine, Changchun University of Traditional Chinese Medicine, Changchun 130021, China.
authors.forename: Guofeng
authors.initials: G
authors.lastname: Li
authors.affiliation: College of Chinese Medicine Materials, Jilin Agricultural University, Changchun 130118, China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Zhu
authors.affiliation: College of Chinese Medicine Materials, Jilin Agricultural University, Changchun 130118, China.
authors.forename: Yugang
authors.initials: Y
authors.lastname: Gao
authors.affiliation: College of Chinese Medicine Materials, Jilin Agricultural University, Changchun 130118, China. Electronic address: lianxuezhang@gmail.com.
authors.forename: Lianxue
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Development Center of Traditional Chinese Medicine and Bioengineering, Changchun University of Traditional Chinese Medicine, Changchun 130117, China. Electronic address: sun_jiaming2000@163.com.
authors.forename: Jiaming
authors.initials: J
authors.lastname: Sun
mesh_terms: D000199:Actins / Q000378:metabolism; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology / Q000627:therapeutic use*; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D027742:Dioscorea / Q000737:chemistry*; D004144:Diosgenin / Q000494:pharmacology / Q000627:therapeutic use*; D006801:Humans; D010766:Phosphorylation; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D051567:Proto-Oncogene Proteins c-vav / Q000378:metabolism*
chemical_list: D000199:Actins; D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; D051567:Proto-Oncogene Proteins c-vav; C093651:VAV2 protein, human; D004144:Diosgenin
keywords: Actin polymerization; Breast cancer; Cdc42; Diosgenin; Migration; Vav2