title: A novel small regulatory RNA enhances cell motility in enterohemorrhagic Escherichia coli.
abstract: Small regulatory RNAs (sRNAs) are conserved among a wide range of bacteria. They modulate the translational efficiency of target mRNAs through base-pairing with the help of RNA chaperone Hfq. The present study identified a novel sRNA, Esr41 (enterohemorrhagic Escherichia coli O157 small RNA #41), from an intergenic region of an enterohemorrhagic E. coli (EHEC) O157:H7 Sakai-specific sequence that is not present in the nonpathogenic E. coli K-12. Esr41 was detected as an RNA molecule approximately 70 nucleotides long with a 3' GC-rich palindrome sequence followed by a long poly(U), which is a characteristic of rho-independent terminators and is also a structural feature required for the action of Hfq. EHEC O157 harboring a multicopy plasmid carrying the esr41 gene increased cell motility and the expression of fliC, a gene encoding a major flagellar component. These results indicate that Esr41 stimulates fliC expression in EHEC O157. Furthermore, the increase in cell motility induced by Esr41 was also observed in the E. coli K-12, suggesting that target genes controlled by Esr41 are present in both EHEC O157 and K-12.
authors.affiliation: Department of Life Science, College of Science, Rikkyo University.
authors.forename: Naoki
authors.initials: N
authors.lastname: Sudo
authors.forename: Akiko
authors.initials: A
authors.lastname: Soma
authors.forename: Akira
authors.initials: A
authors.lastname: Muto
authors.forename: Sunao
authors.initials: S
authors.lastname: Iyoda
authors.forename: Mayumi
authors.initials: M
authors.lastname: Suh
authors.forename: Nanako
authors.initials: N
authors.lastname: Kurihara
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Abe
authors.forename: Toru
authors.initials: T
authors.lastname: Tobe
authors.forename: Yoshitoshi
authors.initials: Y
authors.lastname: Ogura
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Hayashi
authors.forename: Ken
authors.initials: K
authors.lastname: Kurokawa
authors.forename: Makoto
authors.initials: M
authors.lastname: Ohnishi
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Sekine
mesh_terms: D001483:Base Sequence; D048168:Escherichia coli K12 / Q000235:genetics; D019453:Escherichia coli O157 / Q000235:genetics* / Q000472:pathogenicity / Q000502:physiology*; D029968:Escherichia coli Proteins / Q000235:genetics; D005408:Flagellin; D015870:Gene Expression; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009068:Movement / Q000502:physiology; D010957:Plasmids / Q000235:genetics; D012329:RNA, Bacterial / Q000235:genetics*; D038621:Regulatory Sequences, Ribonucleic Acid; D013045:Species Specificity
chemical_list: D029968:Escherichia coli Proteins; C516095:FliC protein, E coli; D012329:RNA, Bacterial; D038621:Regulatory Sequences, Ribonucleic Acid; D005408:Flagellin