title: Photobiological effect of low-level laser irradiation in bovine embryo production system.
abstract: The objectives of this study were to evaluate the effect of low-level laser irradiation (LLLI) on bovine oocyte and granulosa cells metabolism during in vitro maturation (IVM) and further embryo development. Cumulus-oocytes complexes (COCs) were subjected (experimental group) or not (control group) to irradiation with LLLI in a 633-nm wavelength and 1  J/cm2 fluency. The COCs were evaluated after 30 min, 8, 16, and 24 h of IVM. Cumulus cells were evaluated for cell cycle status, mitochondrial activity, and viability (flow cytometry). Oocytes were assessed for meiotic progression status (nuclear staining), cell cycle genes content [real-time polymerase chain reaction (PCR)], and signal transduction status (western blot). The COCs were also in vitro fertilized, and the cleavage and blastocyst rates were assessed. Comparisons among groups were statistically performed with 5% significance level. For cumulus cells, a significant increase in mitochondrial membrane potential and the number of cells progressing through the cycle could be observed. Significant increases on cyclin B and cyclin-dependent kinase (CDK4) levels were also observed. Concerning the oocytes, a significantly higher amount of total mitogen-activated protein kinase was found after 8 h of irradiation, followed by a decrease in all cell cycle genes transcripts, exception made for the CDK4. However, no differences were observed in meiotic progression or embryo production. In conclusion, LLLI is an efficient tool to modulate the granulosa cells and oocyte metabolism.
authors.affiliation: Universidade Federal do ABC, Center of Natural and Human Sciences, Rua Santa Adélia, 166, Bloco A, Torre 3, S642, Santo André, SP, Brazil.
authors.forename: Carlos Alexandre
authors.initials: CA
authors.lastname: Soares
authors.affiliation: Universidade Federal do ABC, Center of Natural and Human Sciences, Rua Santa Adélia, 166, Bloco A, Torre 3, S642, Santo André, SP, Brazil.
authors.forename: Kelly
authors.initials: K
authors.lastname: Annes
authors.affiliation: Universidade Federal do ABC, Center of Natural and Human Sciences, Rua Santa Adélia, 166, Bloco A, Torre 3, S642, Santo André, SP, Brazil.
authors.forename: Thiago Revers
authors.initials: TR
authors.lastname: Dreyer
authors.affiliation: Universidade Federal do ABC, Center of Natural and Human Sciences, Rua Santa Adélia, 166, Bloco A, Torre 3, S642, Santo André, SP, Brazil.
authors.forename: Taciana
authors.initials: T
authors.lastname: Magrini
authors.affiliation: Universidade Federal do ABC, Center of Natural and Human Sciences, Rua Santa Adélia, 166, Bloco A, Torre 3, S642, Santo André, SP, Brazil.
authors.forename: Mayra Trentin
authors.initials: MT
authors.lastname: Sonoda
authors.affiliation: Universidade Federal do ABC, Center of Natural and Human Sciences, Rua Santa Adélia, 166, Bloco A, Torre 3, S642, Santo André, SP, Brazil.
authors.forename: Herculano
authors.initials: H
authors.lastname: da Silva Martinho
authors.affiliation: University of São Paulo, Department of Animal Reproduction, São Paulo/ SP 05508-270, Brazil.
authors.forename: Marcilio
authors.initials: M
authors.lastname: Nichi
authors.affiliation: University of São Paulo, Department of Animal Reproduction, São Paulo/ SP 05508-270, Brazil.
authors.forename: Mayra Elena Ortiz
authors.initials: ME
authors.lastname: d'Ávila Assumpção
authors.affiliation: Universidade Federal do ABC, Center of Natural and Human Sciences, Rua Santa Adélia, 166, Bloco A, Torre 3, S642, Santo André, SP, Brazil.
authors.forename: Marcella Pecora
authors.initials: MP
authors.lastname: Milazzotto
mesh_terms: D000818:Animals; D002417:Cattle; D004622:Embryo, Mammalian / Q000528:radiation effects; D005260:Female; D020869:Gene Expression Profiling; D006107:Granulosa Cells / Q000378:metabolism / Q000502:physiology / Q000528:radiation effects*; D007834:Lasers; D008540:Meiosis / Q000528:radiation effects; D009865:Oocytes / Q000378:metabolism / Q000502:physiology / Q000528:radiation effects*