title: MicroRNA-150 Is up-regulated in extranodal marginal zone lymphoma of MALT type.
abstract: BACKGROUND
  The mechanisms promoting malignant transformation from chronic Helicobacter pylori-gastritis to gastric extranodal marginal zone lymphoma (MALT lymphoma) are insufficiently characterized. This follow-up study aimed to validate candidate microRNAs (miRs) in the process of neoplastic transformation.
  
  
  METHODS
  MicroRNA expression signatures (n=20) were generated for a total of 60 cases of gastric lesions ranging from Wotherspoon 0-5 employing a quantitative real-time polymerase chain reaction (PCR) approach. Morphological and immunohistochemical characterization of the cohort was supplemented by PCR-based immunoglobulin heavy chain recombination studies.
  
  
  RESULTS
  Quantitative expression of miR-150, miR-142.3p, miR-375 and miR-494 was significantly de-regulated in samples from MALT lymphoma compared to those from gastritis.
  
  
  CONCLUSIONS
  The previously reported up-regulation of miR-150 in marginal zone lymphoma of MALT type was verified in an independent cohort of lymphoma samples employing a modified methodology. This further substantiates the role of miR-150 as a potential oncomiR in MALT lymphoma.
authors.affiliation: Institut für Pathologie, Universität zu Lübeck, Ratzeburger Allee 160, D-23538 Lübeck, Deutschland. niklas.gebauer@uksh.de.
authors.forename: Niklas
authors.initials: N
authors.lastname: Gebauer
authors.forename: Johannes
authors.initials: J
authors.lastname: Kuba
authors.forename: Andrea
authors.initials: A
authors.lastname: Senft
authors.forename: Arne
authors.initials: A
authors.lastname: Schillert
authors.forename: Veronica
authors.initials: V
authors.lastname: Bernard
authors.forename: Christoph
authors.initials: C
authors.lastname: Thorns
mesh_terms: D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000378:metabolism; D002908:Chronic Disease; D005756:Gastritis / Q000235:genetics / Q000378:metabolism / Q000382:microbiology; D016481:Helicobacter Infections / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D016480:Helicobacter pylori / Q000302:isolation & purification; D006801:Humans; D007150:Immunohistochemistry; D018442:Lymphoma, B-Cell, Marginal Zone / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D016612:Paraffin Embedding; D015854:Up-Regulation
chemical_list: D035683:MicroRNAs; C533097:MIR150, human
keywords: MALT; MicroRNA; gastritis; lymphoma; malignant transformation