title: Techniques for characterizing cytomegalovirus-encoded miRNAs.
abstract: microRNAs (miRNAs) are small noncoding RNAs that regulate gene expression at the posttranscriptional level, by binding to sites within the 3' untranslated regions of messenger RNA (mRNA) transcripts. The discovery of this completely new mechanism of gene regulation necessitated the development of a variety of techniques to further characterize miRNAs, their expression, and function. In this chapter, we will discuss techniques currently used in the miRNA field to express, detect, and inhibit miRNAs as well as methods used to identify their targets.
authors.affiliation: Vaccine & Gene Therapy Institute, Oregon Health and Sciences University, Beaverton, OR, USA.
authors.forename: Lauren M
authors.initials: LM
authors.lastname: Hook
authors.forename: Igor
authors.initials: I
authors.lastname: Landais
authors.forename: Meaghan H
authors.initials: MH
authors.lastname: Hancock
authors.forename: Jay A
authors.initials: JA
authors.lastname: Nelson
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D003587:Cytomegalovirus / Q000235:genetics* / Q000472:pathogenicity; D003586:Cytomegalovirus Infections / Q000235:genetics / Q000821:virology; D015967:Gene Expression Regulation, Viral; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000302:isolation & purification; D008967:Molecular Biology / Q000379:methods*; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D020413:3' Untranslated Regions; D035683:MicroRNAs; D012333:RNA, Messenger