title: Enhancement of native fluorescence intensity of berberine by (2-hydroxypropyl)-β-cyclodextrin in capillary electrophoresis coupled by laser-induced fluorescence detection: application to quality control of medicinal plants.
abstract: A sensitive method for the determination of berberine, a plant alkaloid, was developed by capillary electrophoresis coupled with laser induced fluorescence detection. With the addition of (2-hydroxypropyl)-β-cyclodextrin (2-HP-β-CD) to the separation electrolyte, the low fluorescence signal intensity of berberine in aqueous medium was enhanced considerably by 162 fold. The optimal background electrolyte was selected as 20mM acetic acid, 35mM 2-HP-β-CD, and 20% methanol at pH 5. The limit of detection of the method for berberine was 15.7ng/mL. The intra and interday repeatabilities of corrected peak areas as RDSs were 1.83% and 3.75%, respectively. The developed method was successfully applied for the determination of berberine contents in Chinese medicinal plants and herbal supplemental tablets.
authors.affiliation: Istanbul Technical University, Department of Chemistry, Maslak, Istanbul, Turkey.
authors.forename: Sesil
authors.initials: S
authors.lastname: Uzaşçı
authors.affiliation: Istanbul Technical University, Department of Chemistry, Maslak, Istanbul, Turkey. Electronic address: erim@itu.edu.tr.
authors.forename: F Bedia
authors.initials: FB
authors.lastname: Erim
mesh_terms: D000073738:2-Hydroxypropyl-beta-cyclodextrin; D001599:Berberine / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D010936:Plant Extracts / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013607:Tablets / Q000737:chemistry; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D010936:Plant Extracts; D013607:Tablets; D047392:beta-Cyclodextrins; D001599:Berberine; D000073738:2-Hydroxypropyl-beta-cyclodextrin
keywords: Alkaloid; Berberine; Capillary electrophoresis; Cyclodextrin; Plant; Tablets