title: Serum metabolomics study of the acute graft rejection in human renal transplantation based on liquid chromatography-mass spectrometry.
abstract: Acute graft rejection is one of the most common and serious postcomplications in renal transplantation. A noninvasive method is needed to specifically monitor acute graft rejection. We investigated metabolic alterations of acute graft rejection in human renal transplantation by applying a metabolomics approach. Sera from 11 acute graft rejection subjects and 16 nonacute graft rejection subjects were analyzed by a nontargeted liquid chromatography-mass spectrometry (LC-MS) metabolomics approach including both hydrophilic interaction chromatography and reversed-phase liquid chromatography separations. Discriminative metabolites of acute graft rejection after transplantation were detected, including creatinine, kynurenine, uric acid, polyunsaturated fatty acid, phosphatidylcholines, sphingomyelins, lysophosphatidylcholines, etc. The lower level of serum dehydroepiandrosterone sulfate was found in the acute graft rejection group before transplantation. The results revealed comprehensive metabolic abnormalities in acute graft rejection. The findings are valuable for the clinic noninvasive diagnosis or therapy of acute graft rejection.
authors.affiliation: Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences , Dalian 116023, China.
authors.forename: Xinjie
authors.initials: X
authors.lastname: Zhao
authors.forename: Jihong
authors.initials: J
authors.lastname: Chen
authors.forename: Lei
authors.initials: L
authors.lastname: Ye
authors.forename: Guowang
authors.initials: G
authors.lastname: Xu
mesh_terms: D000208:Acute Disease; D000328:Adult; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D002853:Chromatography, Liquid / Q000379:methods*; D003404:Creatinine / Q000097:blood; D003937:Diagnosis, Differential; D005231:Fatty Acids, Unsaturated / Q000097:blood; D005260:Female; D006084:Graft Rejection / Q000097:blood / Q000175:diagnosis / Q000378:metabolism*; D006801:Humans; D016030:Kidney Transplantation; D007737:Kynurenine / Q000097:blood; D008244:Lysophosphatidylcholines / Q000097:blood; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D055432:Metabolomics / Q000379:methods*; D008875:Middle Aged; D015999:Multivariate Analysis; D010713:Phosphatidylcholines / Q000097:blood; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013109:Sphingomyelins / Q000097:blood; D014527:Uric Acid / Q000097:blood
chemical_list: D015415:Biomarkers; D005231:Fatty Acids, Unsaturated; D008244:Lysophosphatidylcholines; D010713:Phosphatidylcholines; D013109:Sphingomyelins; D014527:Uric Acid; D007737:Kynurenine; D003404:Creatinine