title: A resorufin-based colorimetric and fluorescent probe for live-cell monitoring of hydrazine.
abstract: We report a novel colorimetric and red-emitting fluorescent probe for hydrazine detection based on resorufin platform. This OFF-ON fluorescent probe shows a large (117nm) red-shifted absorption spectrum and the color changes from colorless to red upon addition of hydrazine in the aqueous solution, which can serve as a "naked-eye" probe for hydrazine. Moreover, this probe also shows a significant fluorescence increase (~16 folds) and excellent linear relationship at physiological pH. Utilizing this sensitive and selective probe, we have successfully detected hydrazine in living cells.
authors.affiliation: State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, China. Electronic address: yongqian@nju.edu.cn.
authors.forename: Yong
authors.initials: Y
authors.lastname: Qian
authors.affiliation: State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Lin
authors.affiliation: State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, China.
authors.forename: Lujing
authors.initials: L
authors.lastname: Han
authors.affiliation: State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Lin
authors.affiliation: State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, China. Electronic address: zhuhl@nju.edu.cn.
authors.forename: Hailiang
authors.initials: H
authors.lastname: Zhu
mesh_terms: D000818:Animals; D001681:Biological Assay / Q000379:methods*; D016466:CHO Cells; D003124:Colorimetry / Q000379:methods*; D006224:Cricetinae; D003412:Cricetulus; D005456:Fluorescent Dyes / Q000737:chemistry; D006834:Hydrazines / Q000032:analysis* / Q000494:pharmacology*; D008422:Materials Testing; D010078:Oxazines / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes; D006834:Hydrazines; D010078:Oxazines; C029424:hydrazine; C014180:resorufin
keywords: Cell imaging; Colorimetric; Fluorescent probe; Hydrazine; Resorufin