title: Bone marrow examination in patients with immune thrombocytopenia: is there anything different in older patients?
abstract: Immune thrombocytopenia (ITP) is a disorder characterised by immune-mediated accelerated platelet destruction and suppressed platelet production. In the bone marrow examinations of patients with ITP, some investigators found megakaryocyte numbers to be increased while others have found them to be normal. Although recent guidelines recommend against bone marrow examinations in typical ITP patients, the recent introduction of thrombopoietin receptor agonists as an effective treatment for ITP has refocused attention on abnormalities of bone marrow megakaryocytes. In this study, we retrospectively analysed the bone marrow aspiration, flow cytometry-CD45 side scatter (SSC) and biopsy results of our patients with ITP by dividing them into two groups according to age (<60 yr and ≥60 yr). Ninety eight newly diagnosed ITP patients were included in the study. CD45 SSC results were recorded as percentages of normoblasts, granulocytes, lymphocytes, monocytes and myeloid/erythroid ratio. Length of the biopsy specimen, cellularity, presence of dysplasia or fibrosis with number, morphology and distribution of megakaryocytes were recorded. In group 1, there were 49 patients. Mean age was 41.31 ± 12.77 yr. In group 2, there were 49 patients. Mean age was 70.78 ± 7.88 yr. Megakaryocyte numbers on bone marrow aspirates were not recorded in most patients, so we could not comment on this point. Flow cytometry results and bone marrow findings were similar between two groups. In conclusion, there is no difference between bone marrow examinations of young and older patients with ITP, and biopsy should not be recommended in typical ITP patients as already mentioned in guidelines.
authors.affiliation: Department of Hematology, School of Medicine, Eskisehir Osmangazi University, Eskisehir, Turkey.
authors.forename: Eren
authors.initials: E
authors.lastname: Gunduz
authors.forename: Banu Kara
authors.initials: BK
authors.lastname: Kivanc
authors.forename: Deniz
authors.initials: D
authors.lastname: Arik
authors.forename: Serap
authors.initials: S
authors.lastname: Isiksoy
authors.forename: Cengiz
authors.initials: C
authors.lastname: Bal
authors.forename: Olga Meltem
authors.initials: OM
authors.lastname: Akay
mesh_terms: D000328:Adult; D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D001706:Biopsy; D001792:Blood Platelets / Q000473:pathology*; D001853:Bone Marrow / Q000473:pathology*; D002452:Cell Count; D005260:Female; D006098:Granulocytes / Q000473:pathology; D006801:Humans; D008214:Lymphocytes / Q000473:pathology; D008297:Male; D008533:Megakaryocytes / Q000473:pathology*; D008875:Middle Aged; D009000:Monocytes / Q000473:pathology; D017410:Practice Guidelines as Topic; D016553:Purpura, Thrombocytopenic, Idiopathic / Q000473:pathology*; D012189:Retrospective Studies
keywords: age; bone marrow biopsy; flow cytometry; immune thrombocytopenia