title: Production of equine herpesvirus 1 recombinant glycoprotein D and development of an agar gel immunodiffusion test for serological diagnosis.
abstract: Equine herpesvirus 1 and 4 (EHV-1 and 4) infect most of the world's horses, causing serious clinical illness. Viral glycoproteins have been identified as the immunodominant antigens that generate the antiviral serological responses to EHV-1 and EHV-4 in infected horses. Here, glycoprotein D of EHV-1 was expressed by a recombinant baculovirus, purified and evaluated by a simple agar gel immunodiffusion test (AGID). Compared with virus neutralization, serological analysis by AGID showed good specificity (100%) and sensitivity (99.5%). The estimated Kappa values for repeatability and reproducibility were satisfactory. Thus, this rapid, inexpensive, simple and highly specific AGID test seems to be a valuable alternative tool for serological detection of antibodies against both EHV-1 and EHV-4.
authors.affiliation: Department of Virology, School of Veterinary Sciences, National University of La Plata, 60 & 118, P.O. Box 296, 1900 La Plata, Buenos Aires, Argentina; National Research Council (CCT-CONICET-La Plata), Argentina. Electronic address: nadiafuentealba@fcv.unlp.edu.ar.
authors.forename: Nadia
authors.initials: N
authors.lastname: Fuentealba
authors.affiliation: Department of Virology, School of Veterinary Sciences, National University of La Plata, 60 & 118, P.O. Box 296, 1900 La Plata, Buenos Aires, Argentina. Electronic address: sguazza@fcv.unlp.edu.ar.
authors.forename: Guillermo
authors.initials: G
authors.lastname: Sguazza
authors.affiliation: Department of Virology, School of Veterinary Sciences, National University of La Plata, 60 & 118, P.O. Box 296, 1900 La Plata, Buenos Aires, Argentina; National Research Council (CCT-CONICET-La Plata), Argentina. Electronic address: scrochimariela@gmail.com.
authors.forename: Mariela
authors.initials: M
authors.lastname: Scrochi
authors.affiliation: Department of Virology, School of Veterinary Sciences, National University of La Plata, 60 & 118, P.O. Box 296, 1900 La Plata, Buenos Aires, Argentina; Argentine Agency for the Promotion of Science and Technology (ANPCyT), Argentina. Electronic address: murguita_@hotmail.com.
authors.forename: Maria
authors.initials: M
authors.lastname: Bravi
authors.affiliation: Department of Histology and Embryology, School of Veterinary Sciences, National University of La Plata, 60 & 118, P.O. Box 296, 1900 La Plata, Buenos Aires, Argentina; Department of Pathology, School of Veterinary Sciences, National University of La Plata, 60 & 118, P.O. Box 296, 1900 La Plata, Buenos Aires, Argentina; National Research Council (CCT-CONICET-La Plata), Argentina. Electronic address: carozanuzzi@gmail.com.
authors.forename: Carolina
authors.initials: C
authors.lastname: Zanuzzi
authors.affiliation: Department of Epidemiology, School of Veterinary Sciences, National University of La Plata, 60 & 118, P.O. Box 296, 1900 La Plata, Buenos Aires, Argentina. Electronic address: sgcorva@fcv.unlp.edu.ar.
authors.forename: Santiago
authors.initials: S
authors.lastname: Corva
authors.affiliation: Department of Pathology, School of Veterinary Sciences, National University of La Plata, 60 & 118, P.O. Box 296, 1900 La Plata, Buenos Aires, Argentina; National Research Council (CCT-CONICET-La Plata), Argentina. Electronic address: ejgimeno@gmail.com.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Gimeno
authors.affiliation: Department of Virology, School of Veterinary Sciences, National University of La Plata, 60 & 118, P.O. Box 296, 1900 La Plata, Buenos Aires, Argentina. Electronic address: peco@fcv.unlp.edu.ar.
authors.forename: Marcelo
authors.initials: M
authors.lastname: Pecoraro
authors.affiliation: Department of Virology, School of Veterinary Sciences, National University of La Plata, 60 & 118, P.O. Box 296, 1900 La Plata, Buenos Aires, Argentina; Scientific Research Commission of the Province of Buenos Aires (CIC-PBA), Argentina. Electronic address: cmgalosi@fcv.unlp.edu.ar.
authors.forename: Cecilia
authors.initials: C
authors.lastname: Galosi
mesh_terms: D000818:Animals; D006023:Glycoproteins / Q000235:genetics / Q000302:isolation & purification; D006566:Herpesviridae Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D004861:Herpesvirus 1, Equid / Q000302:isolation & purification*; D006734:Horse Diseases / Q000175:diagnosis* / Q000821:virology*; D006736:Horses; D005779:Immunodiffusion / Q000379:methods*; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods; D014730:Veterinary Medicine / Q000379:methods*; D014764:Viral Proteins / Q000235:genetics / Q000302:isolation & purification
chemical_list: D006023:Glycoproteins; D011994:Recombinant Proteins; D014764:Viral Proteins
keywords: Agar gel immunodiffusion test; Equine herpesvirus 1; Recombinant glycoprotein D; Virus neutralization