title: Age-related impairment of bones' adaptive response to loading in mice is associated with sex-related deficiencies in osteoblasts but no change in osteocytes.
abstract: Bones adjust their mass and architecture to be sufficiently robust to withstand functional loading by adapting to their strain environment. This mechanism appears less effective with age, resulting in low bone mass. In male and female young adult (17-week-old) and old (19-month-old) mice, we investigated the effect of age in vivo on bones' adaptive response to loading and in vitro in primary cultures of osteoblast-like cells derived from bone cortices. Right tibias were axially loaded on alternate days for 2 weeks. Left tibias were non-loaded controls. In a separate group, the number of sclerostin-positive osteocytes and the number of periosteal osteoblasts were analyzed 24 hours after a single loading episode. The responses to strain of the primary osteoblast-like cells derived from these mice were assessed by EGR2 expression, change in cell number and Ki67 immunofluorescence. In young male and female mice, loading increased trabecular thickness and the number of trabecular connections. Increase in the number of trabecular connections was impaired with age but trabecular thickness was not. In old mice, the loading-related increase in periosteal apposition of the cortex was less than in young ones. Age was associated with a lesser loading-related increase in osteoblast number on the periosteal surface but had no effect on loading-related reduction in the number of sclerostin-positive osteocytes. In vitro, strain-related proliferation of osteoblast-like cells was lower in cells from old than young mice. Cells from aged female mice demonstrated normal entry into the cell cycle but subsequently arrested in G2 phase, reducing strain-related increases in cell number. Thus, in both male and female mice, loading-related adaptive responses are impaired with age. This impairment is different in females and males. The deficit appears to occur in osteoblasts' proliferative responses to strain rather than earlier strain-related responses in the osteocytes.
authors.affiliation: School of Veterinary Science, University of Bristol, Langford, Bristol, UK.
authors.forename: Lee B
authors.initials: LB
authors.lastname: Meakin
authors.forename: Gabriel L
authors.initials: GL
authors.lastname: Galea
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Sugiyama
authors.forename: Lance E
authors.initials: LE
authors.lastname: Lanyon
authors.forename: Joanna S
authors.initials: JS
authors.lastname: Price
mesh_terms: D000367:Age Factors; D000818:Animals; D001835:Body Weight; D001842:Bone and Bones / Q000737:chemistry*; D049109:Cell Proliferation; D005260:Female; D008297:Male; D051379:Mice; D010006:Osteoblasts / Q000166:cytology*; D010011:Osteocytes / Q000166:cytology; D013314:Stress, Mechanical; D013977:Tibia / Q000737:chemistry*
keywords: AGING; MECHANICAL LOADING; OSTEOBLASTS