title: Functional genomics down under: RNAi screening in the Victorian Centre for Functional Genomics.
abstract: The Victorian Centre for Functional Genomics (VCFG) is an RNAi screening facility housed at the Peter MacCallum Cancer Centre in Melbourne, Australia. The Peter Mac is Australia's largest dedicated Cancer Research Institute, home to a team of over 520 scientists that focus on understanding the genetic risk of cancer, the molecular events regulating cancer growth and dissemination and improving detection through new diagnostic tools (www.petermac.org). Peter Mac is a well recognised technology leader and established the VCFG with a view to enabling researchers Australia and New Zealand-wide access to cutting edge functional genomics technology, infrastructure and expertise. This review documents the technology platforms operated within the VCFG and provides insight into the workflows and analysis pipelines currently in operation.
authors.forename: Daniel W
authors.initials: DW
authors.lastname: Thomas
authors.forename: Cathryn M
authors.initials: CM
authors.lastname: Gould
authors.forename: Yanny
authors.initials: Y
authors.lastname: Handoko
authors.affiliation: Victorian Centre for Functional Genomics, Peter MacCallum Cancer Centre, East Melbourne, Victoria, Australia. kaylene.simpson@petermac.org.
authors.forename: Kaylene J
authors.initials: KJ
authors.lastname: Simpson
mesh_terms: D001315:Australia; D023281:Genomics / Q000379:methods / Q000458:organization & administration*; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D035683:MicroRNAs; D009369:Neoplasms / Q000175:diagnosis / Q000235:genetics*; D011786:Quality Control; D034622:RNA Interference; D034741:RNA, Small Interfering; D014162:Transfection / Q000295:instrumentation / Q000379:methods; D057170:Translational Research, Biomedical / Q000295:instrumentation / Q000379:methods / Q000458:organization & administration*; D057188:Workflow
chemical_list: D035683:MicroRNAs; D034741:RNA, Small Interfering