title: Evaluation of ion mobility-mass spectrometry for determining the isomeric heterogeneity of oligosaccharide-alditols derived from bovine submaxillary mucin.
abstract: Rapid separation and independent analysis of isomeric species are needed for the structural characterization of carbohydrates in glycomics research. Ion mobility-mass spectrometry techniques were used to examine a series of isomeric neutral oligosaccharide-alditols derived from bovine submaxillary mucin. Several analytical techniques were employed: (1) off line separation of the oligosaccharide-alditol mixture by HPLC; (2) direct and rapid evaluation of isomeric heterogeneity of oligosaccharides by electrospray ionization-ion mobility-time of flight mass spectrometry; and (3) mobility-selected MS2and MS3to evaluate isomeric mobility peaks by dual gate ion mobility-tandem mass spectrometry. Multiple isomeric ion mobility peaks were observed for the majority of oligosaccharide-alditols, which was achieved on the millisecond time scale after LC separation. Fragmentation spectra obtained from the collision-induced dissociation of isomeric precursor ions could be essentially identical, or dramatically different for a given precursorm/zusing the dual-gate ion mobility quadrupole ion trap mass spectrometer. This further confirmed the need for rapid physical resolution of isomeric precursor species prior to their tandem mass spectral analysis.
authors.affiliation: Department of Chemistry, Washington State University, Pullman, WA, USA.
authors.forename: Hongli
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Cell and Developmental Biology and Program in Structural Biology and Biophysics, University of Colorado, Health Sciences Center, Anschutz Medical Campus, Aurora, CO, USA.
authors.forename: Brad
authors.initials: B
authors.lastname: Bendiak
authors.affiliation: Department of Chemistry, Washington State University, Pullman, WA, USA.
authors.forename: Kimberly
authors.initials: K
authors.lastname: Kaplan
authors.affiliation: Department of Chemistry, Washington State University, Pullman, WA, USA.
authors.forename: Eric
authors.initials: E
authors.lastname: Davis
authors.affiliation: Department of Chemistry, Washington State University, Pullman, WA, USA.
authors.forename: William F
authors.initials: WF
authors.lastname: Siems
authors.affiliation: Department of Chemistry, Washington State University, Pullman, WA, USA.
authors.forename: Herbert H
authors.initials: HH
authors.lastname: Hill
keywords: Bovine submaxillary mucin; Ion mobility-mass spectrometry; Isomeric heterogeneity; Mobility selected fragmentation; Oligosaccharide-alditol