title: Kinetics of the aerobic co-metabolism of 1,1-dichloroethylene by Achromobacter sp.: a novel benzene-grown culture.
abstract: Batch experiments were performed for the aerobic co-metabolism of 1,1-dichloroethylene (1,1-DCE) by Achromobacter sp., identified by gene sequencing of 16S rRNA and grown on benzene. Kinetic models were employed to simulate the co-metabolic degradation of 1,1-DCE, and relevant parameters were obtained by non-linear least squares regression. Benzene at 90 mg L(-1) non-competitively inhibited degradation of 1,1-DCE (from 125 to 1,200 μg L(-1)). The maximum specific utilization (kc) rate and the half-saturation constant (Kc) for 1,1-DCE were 54 ± 0.85 μg h(-1) and 220 ± 6.8 μg L(-1), respectively; the kb and Kb for benzene were 13 ± 0.18 mg h(-1) and 28 ± 0.42 mg L(-1), respectively. This study provides a theoretical basis to predict the natural attenuation when benzene and 1,1-DCE occur as co-contaminants.
authors.affiliation: School of Water Resources and Environment, China University of Geosciences, Beijing, 100083, People's Republic of China.
authors.forename: Shizong
authors.initials: S
authors.lastname: Wang
authors.forename: Qi
authors.initials: Q
authors.lastname: Yang
authors.forename: Luning
authors.initials: L
authors.lastname: Zhang
authors.forename: Yeyao
authors.initials: Y
authors.lastname: Wang
mesh_terms: D042402:Achromobacter / Q000145:classification / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D000332:Aerobiosis; D001554:Benzene / Q000378:metabolism*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004000:Dichloroethylenes / Q000378:metabolism*; D008969:Molecular Sequence Data; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D004000:Dichloroethylenes; D012336:RNA, Ribosomal, 16S; C029297:vinylidene chloride; D001554:Benzene