title: Topical Antinociceptive Effect of Vanillosmopsis arborea Baker on Acute Corneal Pain in Mice.
abstract: This study aimed to assess the possible topical antinociceptive activity of Vanillosmopsis arborea Baker essential oil (EOVA) and to clarify the underlying mechanism, using the acute model of chemical (eye wiping) nociception in mice. EOVA (25 to 200 mg/kg; p.o. and topical) evidenced significant antinociception against chemogenic pain in the test model of formalin-induced neuroinflammatory pain. Local application of 5 M NaCl solution on the corneal surface of the eye produced a significant nociceptive behavior, characterized by eye wiping. The number of eye wipes was counted during the first 30 s. EOVA (25, 50, 100, and 200 mg/kg; p.o. and topical) significantly decreased the number of eye wipes. Naloxone, yohimbine, L-NAME, theophylline, glibenclamide, and ruthenium red had no effect on the antinociceptive effect of EOVA. However, ondansetron, p-chlorophenylalanine methyl ester (PCPA), capsazepine, prazosin, and atropine prevented the antinociception induced by EOVA. These results indicate the topical antinociceptive effect of EOVA and showed that 5-HT, α 1, TRPV1, and central muscarinic receptors might be involved in the antinociceptive effect of EOVA in the acute corneal model of pain in mice.
authors.affiliation: Programa de Pós-Graduação em Bioprospecção Molecular, Universidade Regional do Cariri, 63105-000 Crato, CE, Brazil.
authors.forename: Laura Hévila
authors.initials: LH
authors.lastname: Inocêncio Leite
authors.affiliation: Programa de Pós-Graduação em Bioprospecção Molecular, Universidade Regional do Cariri, 63105-000 Crato, CE, Brazil.
authors.forename: Gerlânia de Oliveira
authors.initials: Gde O
authors.lastname: Leite
authors.affiliation: Programa de Pós-Graduação em Bioprospecção Molecular, Universidade Regional do Cariri, 63105-000 Crato, CE, Brazil.
authors.forename: Thales
authors.initials: T
authors.lastname: Silva Coutinho
authors.affiliation: Programa de Pós-Graduação em Bioprospecção Molecular, Universidade Regional do Cariri, 63105-000 Crato, CE, Brazil.
authors.forename: Severino Denício Gonçalves
authors.initials: SD
authors.lastname: de Sousa
authors.affiliation: Programa de Pós-Graduação em Bioprospecção Molecular, Universidade Regional do Cariri, 63105-000 Crato, CE, Brazil.
authors.forename: Renata Souza
authors.initials: RS
authors.lastname: Sampaio
authors.affiliation: Programa de Pós-Graduação em Bioprospecção Molecular, Universidade Regional do Cariri, 63105-000 Crato, CE, Brazil.
authors.forename: José Galberto Martins
authors.initials: JG
authors.lastname: da Costa
authors.affiliation: Programa de Pós-Graduação em Bioprospecção Molecular, Universidade Regional do Cariri, 63105-000 Crato, CE, Brazil.
authors.forename: Irwin Rose Alencar
authors.initials: IR
authors.lastname: de Menezes
authors.affiliation: Universidade de Fortaleza, Avenida Washington Soares 1321, 60811-905 Fortaleza, CE, Brazil.
authors.forename: Adriana Rolim
authors.initials: AR
authors.lastname: Campos