title: Nitrogen removal pathway of anaerobic ammonium oxidation in on-site aged refuse bioreactor.
abstract: The nitrogen removal pathways and nitrogen-related functional genes in on-site three-stage aged refuse bioreactor (ARB) treating landfill leachate were investigated. It was found that on average 90.0% of CODCr, 97.6% of BOD5, 99.3% of NH4(+)-N, and 81.0% of TN were removed with initial CODCr, BOD5, NH4(+)-N, and TN concentrations ranging from 2323 to 2754, 277 to 362, 1237 to 1506, and 1251 to 1580 mg/L, respectively. Meanwhile, the functional genes amoA, nirS and anammox 16S rRNA gene were found to coexist in every bioreactor, and their relative proportions in each bioreactor were closely related to the pollutant removal performance of the corresponding bioreactor, which indicated the coexistence of multiple nitrogen removal pathways in the ARB. Detection of anammox expression proved the presence of the anammox nitrogen removal pathway during the process of recirculating mature leachate to the on-site ARB, which provides important information for nitrogen management in landfills.
authors.affiliation: Shanghai Key Lab for Urban Ecological Process and Eco-Restoration, Department of Environmental Science & Technology, East China Normal University, Shanghai 200062, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, School of Environmental Engineering and Science, Tongji University, Shanghai 200092, China.
authors.forename: Youcai
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Shanghai Key Lab for Urban Ecological Process and Eco-Restoration, Department of Environmental Science & Technology, East China Normal University, Shanghai 200062, China. Electronic address: bxie@des.ecnu.edu.cn.
authors.forename: Bing
authors.initials: B
authors.lastname: Xie
authors.affiliation: Shanghai Key Lab for Urban Ecological Process and Eco-Restoration, Department of Environmental Science & Technology, East China Normal University, Shanghai 200062, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Peng
authors.affiliation: Shanghai Key Lab for Urban Ecological Process and Eco-Restoration, Department of Environmental Science & Technology, East China Normal University, Shanghai 200062, China.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Hassan
authors.affiliation: Shanghai Key Lab for Urban Ecological Process and Eco-Restoration, Department of Environmental Science & Technology, East China Normal University, Shanghai 200062, China.
authors.forename: Xiaoyuan
authors.initials: X
authors.lastname: Wang
mesh_terms: D064751:Ammonium Compounds / Q000378:metabolism*; D000693:Anaerobiosis; D001673:Biodegradation, Environmental; D019149:Bioreactors / Q000382:microbiology*; D058440:Denitrification; D005798:Genes, Bacterial; D009584:Nitrogen / Q000302:isolation & purification*; D010084:Oxidation-Reduction; D012037:Refuse Disposal / Q000295:instrumentation*; D014874:Water Pollutants, Chemical / Q000302:isolation & purification; D018508:Water Purification / Q000295:instrumentation
chemical_list: D064751:Ammonium Compounds; D014874:Water Pollutants, Chemical; D009584:Nitrogen
keywords: Aged refuse bioreactor; Anammox; Landfill leachate; Nitrogen removal