title: Different influence of Ti, PMMA, UHMWPE, and Co-Cr particles on peripheral blood monocytes during periprosthetic inflammation.
abstract: This study investigated cellular trafficking and inflammatory markers in orthopedic biomaterial particle-challenged human peripheral blood monocytes (PBMCs) using a murine immunodeficiency (SCID) model. Periprosthetic tissues from aseptic loosening patients were transplanted into muscles of SCID mice. PBMCs from the same patients were stimulated in vitro with Ti-6Al-4V, PMMA, UHMWPE, or Co-Cr particles for 3 days before administered intraperitoneally to the periprosthetic tissue-implanted mice. The xenografts were harvested 2 weeks later for histological and molecular analyses. Significant cell infiltration was obvious in the transplanted tissues from mice transfused with Ti-alloy, PMMA and UHMWPE-provoked PBMCs compared to controls, and UHMWPE-provoked PBMCs group accumulated significantly more cells among all groups. There were ubiquitous TRAP+ stained cells in all xenografts from particle-stimulated PBMCs mice. Immunohistochemical staining indicated that significantly more IL-1β and TNF positive cells occurred in Ti and PMMA groups; while the UHMWPE group resulted in stronger positive MCP-1 and IL-6 stains. Polymerase chain reaction (PCR) confirmed overexpression of both IL-1β and TNF in Ti and PMMA-stimulated groups; and more MIP-1α gene expression developed in the UHMWPE group. Overall, different type of orthopedic materials influenced the trafficking ability of particle-activated PBMCs which may depend on upregulation of various proinflammatory cytokines and chemokines.
authors.affiliation: Department of Orthopaedics, Affiliated Hospital to Binzhou Medical College, Binzhou, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Zhang
authors.forename: Shang-You
authors.initials: SY
authors.lastname: Yang
authors.forename: Shuye
authors.initials: S
authors.lastname: Yang
authors.forename: Ling
authors.initials: L
authors.lastname: Bai
authors.forename: Peng
authors.initials: P
authors.lastname: Li
authors.forename: Dong
authors.initials: D
authors.lastname: Liu
authors.forename: John R
authors.initials: JR
authors.lastname: Schurman
authors.forename: Paul H
authors.initials: PH
authors.lastname: Wooley
mesh_terms: D000818:Animals; D002857:Chromium; D003035:Cobalt; D016207:Cytokines / Q000378:metabolism; D020869:Gene Expression Profiling; D064593:Heterografts; D007249:Inflammation; D051379:Mice; D016513:Mice, SCID; D009000:Monocytes / Q000187:drug effects* / Q000378:metabolism; D011095:Polyethylenes / Q000494:pharmacology*; D016133:Polymerase Chain Reaction; D019904:Polymethyl Methacrylate / Q000494:pharmacology*; D019736:Prostheses and Implants / Q000009:adverse effects*; D014025:Titanium / Q000494:pharmacology*
chemical_list: D016207:Cytokines; D011095:Polyethylenes; C111601:ultra-high molecular weight polyethylene; D002857:Chromium; D003035:Cobalt; D019904:Polymethyl Methacrylate; D014025:Titanium
keywords: aseptic loosening; cell trafficking; inflammation; monocytes; wear debris