title: One-step pipetting and assembly of encoded chemical-laden microparticles for high-throughput multiplexed bioassays.
abstract: One quantitative liquid handling method in conventional assay processes is pipetting, which delivers a precise volume of one sample at a time. As this process becomes laborious and time-consuming as the number of samples increases, researchers in individual laboratories need a way to conduct large-scale assays in a reasonable amount of time and at an affordable cost. Here we report a novel handling technique of chemical substances termed 'partipetting', which allows the one-step pipetting of various chemical-laden hydrogels. We pipette and assemble various types of encoded chemical-laden microparticles in microwell arrays in parallel. The combination of this heterogeneous particle chip and a cell chip induces the release of the chemicals from the hydrogels and, eventually, the chemicals treat the targets. Based on bioassay applications using partipetting, we show its capability in large-scale bioassays, without the need for high-throughput bioassay resources, owing to a reduction in the assay costs and time.
authors.affiliation: 1] Center for Nanoparticle Research, Institute for Basic Science (IBS), Seoul National University, Gwanakro, Gwanak-gu, Seoul 151-744, South Korea [2] Department of Electrical and Computer Engineering, Seoul National University, Gwanakro, Gwanak-gu, Seoul 151-744, South Korea [3] Inter-university Semiconductor Research Center, Seoul National University, Gwanakro, Gwanak-gu, Seoul 151-744, South Korea [4].
authors.forename: Su
authors.initials: S
authors.lastname: Eun Chung
authors.affiliation: 1] Center for Nanoparticle Research, Institute for Basic Science (IBS), Seoul National University, Gwanakro, Gwanak-gu, Seoul 151-744, South Korea [2] Department of Electrical and Computer Engineering, Seoul National University, Gwanakro, Gwanak-gu, Seoul 151-744, South Korea [3] Inter-university Semiconductor Research Center, Seoul National University, Gwanakro, Gwanak-gu, Seoul 151-744, South Korea [4].
authors.forename: Jiyun
authors.initials: J
authors.lastname: Kim
authors.affiliation: 1] Center for Nanoparticle Research, Institute for Basic Science (IBS), Seoul National University, Gwanakro, Gwanak-gu, Seoul 151-744, South Korea [2] Inter-university Semiconductor Research Center, Seoul National University, Gwanakro, Gwanak-gu, Seoul 151-744, South Korea [3] Interdisciplinary program of Bioengineering, Seoul National University, Gwanakro 1, Gwanak-gu, Seoul 151-744, South Korea.
authors.forename: Dong
authors.initials: D
authors.lastname: Yoon Oh
authors.affiliation: 1] Center for Nanoparticle Research, Institute for Basic Science (IBS), Seoul National University, Gwanakro, Gwanak-gu, Seoul 151-744, South Korea [2] Department of Electrical and Computer Engineering, Seoul National University, Gwanakro, Gwanak-gu, Seoul 151-744, South Korea [3] Inter-university Semiconductor Research Center, Seoul National University, Gwanakro, Gwanak-gu, Seoul 151-744, South Korea.
authors.forename: Younghoon
authors.initials: Y
authors.lastname: Song
authors.affiliation: Department of Electrical and Computer Engineering, Seoul National University, Gwanakro, Gwanak-gu, Seoul 151-744, South Korea.
authors.forename: Sung
authors.initials: S
authors.lastname: Hoon Lee
authors.affiliation: Department of Electrical and Computer Engineering, Seoul National University, Gwanakro, Gwanak-gu, Seoul 151-744, South Korea.
authors.forename: Seungki
authors.initials: S
authors.lastname: Min
authors.affiliation: 1] Center for Nanoparticle Research, Institute for Basic Science (IBS), Seoul National University, Gwanakro, Gwanak-gu, Seoul 151-744, South Korea [2] Department of Electrical and Computer Engineering, Seoul National University, Gwanakro, Gwanak-gu, Seoul 151-744, South Korea [3] Inter-university Semiconductor Research Center, Seoul National University, Gwanakro, Gwanak-gu, Seoul 151-744, South Korea.
authors.forename: Sunghoon
authors.initials: S
authors.lastname: Kwon
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D001681:Biological Assay / Q000191:economics / Q000295:instrumentation* / Q000379:methods; D002460:Cell Line; D057166:High-Throughput Screening Assays / Q000191:economics / Q000295:instrumentation* / Q000379:methods; D006801:Humans
chemical_list: D000970:Antineoplastic Agents