title: Experimental phage therapy against lethal lung-derived septicemia caused by Staphylococcus aureus in mice.
abstract: Nosocomial respiratory infections caused by methicillin-resistant Staphylococcus aureus (MRSA) can progress to lethal systemic infections. Bacteriophage (phage) therapy is expected to be effective against these critical infections. Previously, phage S13' was proposed as a potential therapeutic phage. We here examined phage treatment in a mouse model of lung-derived septicemia using phage S13'. Intraperitoneal phage administration at 6 h postinfection reduced the severity of infection and rescued the infected mice. Phage S13' can efficiently lyse hospital-acquired MRSA strains causing pneumonia-associated bacteremia in vitro. Thus, phage therapy may be a possible therapeutic intervention in staphylococcal lung-derived septicemia.
authors.affiliation: Department of Microbiology and Infection, Faculty of Medicine, Kochi University, Kochi, Japan; Clinical Laboratory, Kochi University Hospital, Kochi, Japan.
authors.forename: Iyo
authors.initials: I
authors.lastname: Takemura-Uchiyama
authors.affiliation: Department of Microbiology and Infection, Faculty of Medicine, Kochi University, Kochi, Japan; Center for Innovative and Translational Medicine, Kochi University, Kochi, Japan.
authors.forename: Jumpei
authors.initials: J
authors.lastname: Uchiyama
authors.affiliation: Department of Pathology, Faculty of Medicine, Kochi University, Kochi, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Osanai
authors.affiliation: Clinical Laboratory, Kochi University Hospital, Kochi, Japan.
authors.forename: Norihito
authors.initials: N
authors.lastname: Morimoto
authors.affiliation: Clinical Laboratory, Kochi University Hospital, Kochi, Japan.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Asagiri
authors.affiliation: Science Research Center, Kochi University, Kochi, Japan.
authors.forename: Takako
authors.initials: T
authors.lastname: Ujihara
authors.affiliation: Department of Microbiology and Infection, Faculty of Medicine, Kochi University, Kochi, Japan; Center for Innovative and Translational Medicine, Kochi University, Kochi, Japan.
authors.forename: Masanori
authors.initials: M
authors.lastname: Daibata
authors.affiliation: Clinical Laboratory, Kochi University Hospital, Kochi, Japan.
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Sugiura
authors.affiliation: Department of Microbiology and Infection, Faculty of Medicine, Kochi University, Kochi, Japan; Center for Innovative and Translational Medicine, Kochi University, Kochi, Japan. Electronic address: matuzaki@kochi-u.ac.jp.
authors.forename: Shigenobu
authors.initials: S
authors.lastname: Matsuzaki
mesh_terms: D000818:Animals; D001691:Biological Therapy; D003428:Cross Infection / Q000382:microbiology / Q000628:therapy; D004195:Disease Models, Animal; D008168:Lung / Q000382:microbiology / Q000473:pathology; D055624:Methicillin-Resistant Staphylococcus aureus / Q000821:virology*; D051379:Mice; D018805:Sepsis / Q000628:therapy*; D013203:Staphylococcal Infections / Q000628:therapy*; D013204:Staphylococcus Phages
keywords: Bacteriophage; Mouse; Podoviridae; Therapeutic; Therapy