title: Interaction of porcine circovirus type 2 and Mycoplasma hyopneumoniae vaccines on dually infected pigs.
abstract: The objective of this study was to determine the effects of porcine circovirus type 2 (PCV2) and Mycoplasma hyopneumoniae vaccinations on disease severity in an experimental PCV2-M. hyopneumoniae dual challenge model. Vaccine effectiveness was evaluated using microbiological (PCV2 viremia and M. hyopneumoniae nasal shedding), immunological (neutralizing antibodies and interferon-γ-secreting cells), and pathological (gross lung lesions, histopathologic pulmonary and lymphoid lesions, and the presence of PCV2 antigen and M. hyopneumoniae DNA within the lesions) evaluations. Although M. hyopneumoniae potentiates the severity of PCV2-associated lesions and lesion-associated PCV2 antigen in dually challenged pigs, vaccination against M. hyopneumoniae alone did not reduce PCV2 viremia, PCV2-induced lesions, or PCV2 antigen in dually challenged pigs. In addition, vaccination against PCV2 did not reduce the nasal shedding of M. hyopneumoniae, the M. hyopneumoniae-induced pulmonary lesions or the lesion-associated M. hyopneumoniae DNA in dually challenged pigs. Dual challenge with PCV2 and M. hyopneumoniae did not interfere with the induction of active immunity induced by a previous single vaccination for either PCV2 or M. hyopneumoniae. The results of this study demonstrated that (i) vaccination against M. hyopneumoniae alone did not decrease the potentiation of PCV2-induced lesions by M. hyopneumoniae and (ii) vaccination against PCV2 alone decreased the potentiation of PCV2-induced lesions by M. hyopneumoniae in dually challenged pigs.
authors.affiliation: Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 151-742, Republic of Korea.
authors.forename: Hwi Won
authors.initials: HW
authors.lastname: Seo
authors.affiliation: Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 151-742, Republic of Korea.
authors.forename: Su-Jin
authors.initials: SJ
authors.lastname: Park
authors.affiliation: Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 151-742, Republic of Korea.
authors.forename: Changhoon
authors.initials: C
authors.lastname: Park
authors.affiliation: Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 151-742, Republic of Korea. Electronic address: swine@snu.ac.kr.
authors.forename: Chanhee
authors.initials: C
authors.lastname: Chae
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D057138:Bacterial Shedding; D001428:Bacterial Vaccines / Q000276:immunology*; D018173:Circoviridae Infections / Q000517:prevention & control* / Q000662:veterinary; D017925:Circovirus; D060085:Coinfection / Q000382:microbiology / Q000662:veterinary / Q000821:virology; D004269:DNA, Bacterial / Q000302:isolation & purification; D004279:DNA, Viral / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D007371:Interferon-gamma / Q000276:immunology; D008168:Lung / Q000473:pathology; D045705:Mycoplasma hyopneumoniae; D045729:Pneumonia of Swine, Mycoplasmal / Q000517:prevention & control*; D034421:Sus scrofa; D013552:Swine; D014765:Viral Vaccines / Q000276:immunology*; D014766:Viremia
chemical_list: D000907:Antibodies, Bacterial; D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D001428:Bacterial Vaccines; D004269:DNA, Bacterial; D004279:DNA, Viral; D007074:Immunoglobulin G; D014765:Viral Vaccines; D007371:Interferon-gamma
keywords: Mycoplasma hyopneumoniae; Porcine circovirus type 2; Porcine circovirus-associated disease; Porcine respiratory disease complex; Vaccine