title: Nanoscale engineering of low-fouling surfaces through polydopamine immobilisation of zwitterionic peptides.
abstract: We report a versatile approach for the design of substrate-independent low-fouling surfaces via mussel-inspired immobilisation of zwitterionic peptides. Using mussel-inspired polydopamine (PDA) coatings, zwitterionic glutamic acid- and lysine-based peptides were immobilised on various substrates, including noble metals, metal oxides, polymers, and semiconductors. The variation of surface chemistry and surface wettability upon surface treatment was monitored with X-ray photoelectron spectroscopy (XPS) and water contact angle measurements. Following peptide immobilisation, the surfaces became more hydrophilic due to the strong surface hydration compared with PDA-coated surfaces. The peptide-functionalised surfaces showed resistance to human blood serum adsorption and also effectively prevented the adhesion of gram-negative and gram-positive bacteria (i.e., Escherichia coli and Staphylococcus epidermidis) and mammalian cells (i.e., NIH 3T3 mouse embryonic fibroblast cells). The versatility of mussel-inspired chemistry combined with the unique biological nature and tunability of peptides allows for the design of low-fouling surfaces, making this a promising coating technique for various applications.
authors.affiliation: Department of Chemical and Biomolecular Engineering, The University of Melbourne, Parkville, Victoria 3010, Australia. fcaruso@unimelb.edu.au.
authors.forename: Jiwei
authors.initials: J
authors.lastname: Cui
authors.forename: Yi
authors.initials: Y
authors.lastname: Ju
authors.forename: Kang
authors.initials: K
authors.lastname: Liang
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Ejima
authors.forename: Samuel
authors.initials: S
authors.lastname: Lörcher
authors.forename: Katelyn T
authors.initials: KT
authors.lastname: Gause
authors.forename: Joseph J
authors.initials: JJ
authors.lastname: Richardson
authors.forename: Frank
authors.initials: F
authors.lastname: Caruso
mesh_terms: D000327:Adsorption; D000595:Amino Acid Sequence; D000818:Animals; D001422:Bacterial Adhesion; D002448:Cell Adhesion; D004926:Escherichia coli / Q000502:physiology; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D055767:Immobilized Proteins / Q000737:chemistry / Q000378:metabolism; D007211:Indoles / Q000737:chemistry*; D051379:Mice; D041681:NIH 3T3 Cells; D010455:Peptides / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D044967:Serum / Q000737:chemistry; D013212:Staphylococcus epidermidis / Q000502:physiology; D013499:Surface Properties
chemical_list: D055767:Immobilized Proteins; D007211:Indoles; D010455:Peptides; D011108:Polymers; C568283:polydopamine