title: Determination of erythromycin in medicated salmonid fish feed by liquid chromatography and UV spectroscopy.
abstract: A simple, robust LC-UV method was developed to assay erythromycin in medicated salmonid feed. In this method, erythromycin was extracted from feed with acetonitrile and water, cleaned up by SPE, evaporated to dryness, reconstituted, and analyzed by LC-UV. The resulting method produced high accuracy, 82-90%, for both salmon and trout feed that represented varied pellet sizes and ingredient amounts. The intraday and interday precisions, at < or = 6 and 5%, respectively, indicated the method's good repeatability. Calibration was linear over the range of drug concentrations typically used in medicated feed. An independent analyst validation further demonstrated the repeatability of the method. The developed method could support the drug approval process for erythromycin in medicated salmonid fish feed.
authors.forename: Hemakanthi G
authors.initials: HG
authors.lastname: De Alwis
authors.forename: Lauren
authors.initials: L
authors.lastname: Girard
authors.forename: Philip J
authors.initials: PJ
authors.lastname: Kijak
authors.forename: Nathan
authors.initials: N
authors.lastname: Rummel
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000737:chemistry; D002138:Calibration; D002853:Chromatography, Liquid; D004350:Drug Residues / Q000032:analysis; D004917:Erythromycin / Q000032:analysis* / Q000737:chemistry; D005506:Food Contamination / Q000032:analysis*; D015203:Reproducibility of Results; D012474:Salmon; D013056:Spectrophotometry, Ultraviolet; D014867:Water / Q000737:chemistry
chemical_list: D000097:Acetonitriles; D000900:Anti-Bacterial Agents; D014867:Water; D004917:Erythromycin; C032159:acetonitrile