title: Mangiferin has an additive effect on the apoptotic properties of hesperidin in Cyclopia sp. tea extracts.
abstract: A variety of biological pro-health activities have been reported for mangiferin and hesperidin, two major phenolic compounds of Honeybush (Cyclopia sp.) tea extracts. Given their increasing popularity, there is a need for understanding the mechanisms underlying the biological effects of these compounds. In this study, we used real-time cytotoxicity cellular analysis of the Cyclopia sp. extracts on HeLa cells and found that the higher hesperidin content in non-fermented "green" extracts correlated with their higher cytotoxicity compared to the fermented extracts. We also found that mangiferin had a modulatory effect on the apoptotic effects of hesperidin. Quantitative PCR analysis of hesperidin-induced changes in apoptotic gene expression profile indicated that two death receptor pathway members, TRADD and TRAMP, were up regulated. The results of this study suggest that hesperidin mediates apoptosis in HeLa cells through extrinsic pathway for programmed cell death.
authors.affiliation: Department of Biology and Pharmaceutical Botany, Medical University of Gdansk, Gdansk, Poland.
authors.forename: Rafal
authors.initials: R
authors.lastname: Bartoszewski
authors.affiliation: Department of Biology and Pharmaceutical Botany, Medical University of Gdansk, Gdansk, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Hering
authors.affiliation: Department of Toxicology, Medical University of Gdansk, Gdansk, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Marszałł
authors.affiliation: Department of Biology and Pharmaceutical Botany, Medical University of Gdansk, Gdansk, Poland.
authors.forename: Justyna
authors.initials: J
authors.lastname: Stefanowicz Hajduk
authors.affiliation: Department of Inorganic Chemistry, Medical University of Gdansk, Gdansk, Poland.
authors.forename: Sylwia
authors.initials: S
authors.lastname: Bartoszewska
authors.affiliation: Department of Neurology, University of Alabama at Birmingham, Birmingham, Alabama, United States of America.
authors.forename: Niren
authors.initials: N
authors.lastname: Kapoor
authors.affiliation: Department of Biology and Pharmaceutical Botany, Medical University of Gdansk, Gdansk, Poland.
authors.forename: Kinga
authors.initials: K
authors.lastname: Kochan
authors.affiliation: Department of Biology and Pharmaceutical Botany, Medical University of Gdansk, Gdansk, Poland.
authors.forename: Renata
authors.initials: R
authors.lastname: Ochocka
mesh_terms: D000970:Antineoplastic Agents / Q000032:analysis / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D053998:Cyclopia Plant / Q000737:chemistry*; D004357:Drug Synergism; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006367:HeLa Cells; D006569:Hesperidin / Q000032:analysis / Q000494:pharmacology*; D006801:Humans; D010936:Plant Extracts / Q000737:chemistry*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D053199:TNF Receptor-Associated Death Domain Protein / Q000235:genetics; D044004:Xanthones / Q000032:analysis / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D010936:Plant Extracts; D012333:RNA, Messenger; D053199:TNF Receptor-Associated Death Domain Protein; D044004:Xanthones; C013592:mangiferin; D006569:Hesperidin