title: Structural characterization and antioxidant activities of polysaccharides from Citrus aurantium L.
abstract: Three polysaccharide fractions were obtained from Citrus aurantium L. (CAL) by sequential extraction with cold water, hot water, and 1.0M NaOH, respectively. The fractions were denoted CALA, CALB, and CALC. Structural characterization was conducted by physicochemical property, FTIR, and SEM analyses. Antioxidant activities in vivo and in vitro were also evaluated. CALB, which showed the highest activity, was further isolated to afford four purified polysaccharides (CALB-1-4) by various ion exchange and gel-filtration chromatography. Meanwhile, the purified polysaccharides were subjected to composition analysis and screened by antioxidant activity in vitro. Among the four purified polysaccharides, CALB-3 had the highest antioxidant activity and its structure was analyzed by FTIR, SEM and AFM microscopy. Overall, these results indicated that polysaccharides from CAL had potential therapeutic applications in the medical and food industries because of their antioxidant activities.
authors.affiliation: Key Laboratory of Chinese Materia Medica (Heilongjiang University of Chinese Medicine), Ministry of Education, Harbin 150040, China.
authors.forename: Qiu Hong
authors.initials: QH
authors.lastname: Wang
authors.affiliation: Key Laboratory of Chinese Materia Medica (Heilongjiang University of Chinese Medicine), Ministry of Education, Harbin 150040, China.
authors.forename: Zun Peng
authors.initials: ZP
authors.lastname: Shu
authors.affiliation: Key Laboratory of Chinese Materia Medica (Heilongjiang University of Chinese Medicine), Ministry of Education, Harbin 150040, China.
authors.forename: Bing Qing
authors.initials: BQ
authors.lastname: Xu
authors.affiliation: Key Laboratory of Chinese Materia Medica (Heilongjiang University of Chinese Medicine), Ministry of Education, Harbin 150040, China.
authors.forename: Na
authors.initials: N
authors.lastname: Xing
authors.affiliation: Key Laboratory of Chinese Materia Medica (Heilongjiang University of Chinese Medicine), Ministry of Education, Harbin 150040, China.
authors.forename: Wen Juan
authors.initials: WJ
authors.lastname: Jiao
authors.affiliation: Key Laboratory of Chinese Materia Medica (Heilongjiang University of Chinese Medicine), Ministry of Education, Harbin 150040, China.
authors.forename: Bing You
authors.initials: BY
authors.lastname: Yang
authors.affiliation: Key Laboratory of Chinese Materia Medica (Heilongjiang University of Chinese Medicine), Ministry of Education, Harbin 150040, China. Electronic address: hxkuang@hotmail.com.
authors.forename: Hai Xue
authors.initials: HX
authors.lastname: Kuang
mesh_terms: D000975:Antioxidants / Q000008:administration & dosage / Q000737:chemistry* / Q000302:isolation & purification; D002850:Chromatography, Gel; D002957:Citrus / Q000737:chemistry*; D016166:Free Radical Scavengers; D006801:Humans; D010084:Oxidation-Reduction; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D011134:Polysaccharides / Q000008:administration & dosage / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D010936:Plant Extracts; D011134:Polysaccharides
keywords: Antioxidant activity; Citrus aurantium L.; Polysaccharide; Structural characterization