title: Analysis of specific proteolytic digestion of the peptidoglutaminase-asparaginase of koji molds.
abstract: AsGahB, a peptidoglutaminase-asparaginase acting as the main glutaminase in Aspergillus sojae, was previously purified from the cytoplasm of overexpressing strains. Here, we found that specific proteolytic digestion of AsGahB by extracellular proteases of koji molds is similar to that of AsGahA which exists in proteolytic form under solid-state culture.
authors.affiliation: Noda Institute for Scientific Research, 399 Noda, Noda, Chiba 278-0037, Japan. Electronic address: koito@mail.kikkoman.co.jp.
authors.forename: Kotaro
authors.initials: K
authors.lastname: Ito
authors.affiliation: Noda Institute for Scientific Research, 399 Noda, Noda, Chiba 278-0037, Japan.
authors.forename: Yasuji
authors.initials: Y
authors.lastname: Koyama
mesh_terms: D000595:Amino Acid Sequence; D001215:Asparaginase / Q000737:chemistry* / Q000302:isolation & purification; D001230:Aspergillus / Q000737:chemistry / Q000201:enzymology*; D005656:Fungal Proteins / Q000737:chemistry* / Q000302:isolation & purification; D007527:Isoenzymes / Q000737:chemistry / Q000302:isolation & purification; D008969:Molecular Sequence Data; D059748:Proteolysis
chemical_list: D005656:Fungal Proteins; D007527:Isoenzymes; D001215:Asparaginase
keywords: Aspergillus sojae; Glutamate; Glutaminase; Soy sauce; Specific proteolytic digestion