title: Antibiotic-decorated titanium with enhanced antibacterial activity through adhesive polydopamine for dental/bone implant.
abstract: Implant-associated infections, which are normally induced by microbial adhesion and subsequent biofilm formation, are a major cause of morbidity and mortality. Therefore, practical approaches to prevent implant-associated infections are in great demand. Inspired by adhesive proteins in mussels, here we have developed a novel antibiotic-decorated titanium (Ti) material with enhanced antibacterial activity. In this study, Ti substrate was coated by one-step pH-induced polymerization of dopamine followed by immobilization of the antibiotic cefotaxime sodium (CS) onto the polydopamine-coated Ti through catechol chemistry. Contact angle measurement and X-ray photoelectron spectroscopy confirmed the presence of CS grafted on the Ti surface. Our results demonstrated that the antibiotic-grafted Ti substrate showed good biocompatibility and well-behaved haemocompatibility. In addition, the antibiotic-grafted Ti could effectively prevent adhesion and proliferation of Escherichia coli (Gram-negative) and Streptococcus mutans (Gram-positive). Moreover, the inhibition of biofilm formation on the antibiotic-decorated Ti indicated that the grafted CS could maintain its long-term antibacterial activity. This modified Ti substrate with enhanced antibacterial activity holds great potential as implant material for applications in dental and bone graft substitutes.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Laboratory of Interdisciplinary Studies, Peking University, , Beijing 100081, People's Republic of China.
authors.forename: Shu
authors.initials: S
authors.lastname: He
authors.forename: Ping
authors.initials: P
authors.lastname: Zhou
authors.forename: Linxin
authors.initials: L
authors.lastname: Wang
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Xiong
authors.forename: Yifei
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yi
authors.initials: Y
authors.lastname: Deng
authors.forename: Shicheng
authors.initials: S
authors.lastname: Wei
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry*; D001422:Bacterial Adhesion; D018441:Biofilms / Q000254:growth & development*; D002439:Cefotaxime / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D015921:Dental Implants; D004926:Escherichia coli / Q000502:physiology*; D006801:Humans; D008422:Materials Testing; D013295:Streptococcus mutans / Q000502:physiology*; D014025:Titanium / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D020099:Coated Materials, Biocompatible; D015921:Dental Implants; D014025:Titanium; D002439:Cefotaxime
keywords: antibacterial activity; antibiotic; implant; polydopamine; titanium