title: Coilin association with Box C/D scaRNA suggests a direct role for the Cajal body marker protein in scaRNP biogenesis.
abstract: Spliceosomal small nuclear ribonucleoproteins (snRNPs) are enriched in the Cajal body (CB). Guide RNAs, known as small Cajal body-specific RNAs (scaRNAs), direct modification of the small nuclear RNA (snRNA) component of the snRNP. The protein WRAP53 binds a sequence motif (the CAB box) found in many scaRNAs and the RNA component of telomerase (hTR) and targets these RNAs to the CB. We have previously reported that coilin, the CB marker protein, associates with certain non-coding RNAs. For a more comprehensive examination of the RNAs associated with coilin, we have sequenced the RNA isolated from coilin immunocomplexes. A striking preferential association of coilin with the box C/D scaRNAs 2 and 9, which lack a CAB box, was observed. This association varied by treatment condition and WRAP53 knockdown. In contrast, reduction of WRAP53 did not alter the level of coilin association with hTR. Additional studies showed that coilin degrades/processes scaRNA 2 and 9, associates with active telomerase and can influence telomerase activity. These findings suggest that coilin plays a novel role in the biogenesis of box C/D scaRNPs and telomerase.
authors.affiliation: Department of Biochemistry, The University of Mississippi Medical Center, Jackson, MS 39216-4505, USA.
authors.forename: Isioma I
authors.initials: II
authors.lastname: Enwerem
authors.forename: Venkatramreddy
authors.initials: V
authors.lastname: Velma
authors.forename: Hanna J
authors.initials: HJ
authors.lastname: Broome
authors.forename: Marija
authors.initials: M
authors.lastname: Kuna
authors.forename: Rowshan A
authors.initials: RA
authors.lastname: Begum
authors.forename: Michael D
authors.initials: MD
authors.lastname: Hebert
keywords: Cajal body; Coilin; snRNA