title: A comprehensive approach to determining BER capacities and their change with aging in Drosophila melanogaster mitochondria by oligonucleotide microarray.
abstract: DNA repair mechanisms are key components for the maintenance of the essential mitochondrial genome. Among them, base excision repair (BER) processes, dedicated in part to oxidative DNA damage, are individually well known in mitochondria. However, no large view of these systems in differential physiological conditions is available yet. Combining the use of pure mitochondrial fractions and a multiplexed oligonucleotide cleavage assay on a microarray, we demonstrated that a large range of glycosylase activities were present in Drosophila mitochondria. Most of them were quantitatively different from their nuclear counterpart. Moreover, these activities were modified during aging.
authors.affiliation: Clermont Université, Université Blaise Pascal, EA 4645, Réparation du Génome Mitochondrial normal et pathologique, BP 10448, F-63000 Clermont-Ferrand, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Garreau-Balandier
authors.affiliation: Clermont Université, Université Blaise Pascal, EA 4645, Réparation du Génome Mitochondrial normal et pathologique, BP 10448, F-63000 Clermont-Ferrand, France.
authors.forename: Mathilde
authors.initials: M
authors.lastname: Lefebvre
authors.affiliation: Laboratoire Lésions des Acides Nucléiques, CEA, DSM, INAC, SCIB, UMR-E3 CEA/UJF-Grenoble 1, 17 Rue des Martyrs, F-38054 Grenoble Cedex 9, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Jacquard
authors.affiliation: Laboratoire Lésions des Acides Nucléiques, CEA, DSM, INAC, SCIB, UMR-E3 CEA/UJF-Grenoble 1, 17 Rue des Martyrs, F-38054 Grenoble Cedex 9, France.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Caillat
authors.affiliation: Clermont Université, Université Blaise Pascal, EA 4645, Réparation du Génome Mitochondrial normal et pathologique, BP 10448, F-63000 Clermont-Ferrand, France.
authors.forename: Luis
authors.initials: L
authors.lastname: Cruz-Rodriguez
authors.affiliation: Clermont Université, Université Blaise Pascal, EA 4645, Réparation du Génome Mitochondrial normal et pathologique, BP 10448, F-63000 Clermont-Ferrand, France.
authors.forename: Layal
authors.initials: L
authors.lastname: Ishak
authors.affiliation: Clermont Université, Université Blaise Pascal, EA 4645, Réparation du Génome Mitochondrial normal et pathologique, BP 10448, F-63000 Clermont-Ferrand, France.
authors.forename: Virginie
authors.initials: V
authors.lastname: Agier
authors.affiliation: Clermont Université, Université Blaise Pascal, EA 4645, Réparation du Génome Mitochondrial normal et pathologique, BP 10448, F-63000 Clermont-Ferrand, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Morel
authors.affiliation: Clermont Université, Université Blaise Pascal, EA 4645, Réparation du Génome Mitochondrial normal et pathologique, BP 10448, F-63000 Clermont-Ferrand, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Lachaume
authors.affiliation: Clermont Université, Université Blaise Pascal, EA 4645, Réparation du Génome Mitochondrial normal et pathologique, BP 10448, F-63000 Clermont-Ferrand, France.
authors.forename: Pascal
authors.initials: P
authors.lastname: Dubessay
authors.affiliation: Laboratoire Lésions des Acides Nucléiques, CEA, DSM, INAC, SCIB, UMR-E3 CEA/UJF-Grenoble 1, 17 Rue des Martyrs, F-38054 Grenoble Cedex 9, France.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Sauvaigo
authors.affiliation: Clermont Université, Université Blaise Pascal, EA 4645, Réparation du Génome Mitochondrial normal et pathologique, BP 10448, F-63000 Clermont-Ferrand, France.
authors.forename: Serge
authors.initials: S
authors.lastname: Alziari
authors.affiliation: Clermont Université, Université Blaise Pascal, EA 4645, Réparation du Génome Mitochondrial normal et pathologique, BP 10448, F-63000 Clermont-Ferrand, France. Electronic address: patrick.vernet@univ-bpclermont.fr.
authors.forename: Patrick
authors.initials: P
authors.lastname: Vernet
mesh_terms: D000375:Aging; D000818:Animals; D018736:DNA Adducts / Q000378:metabolism; D045647:DNA Glycosylases / Q000378:metabolism; D004260:DNA Repair; D029721:Drosophila Proteins / Q000378:metabolism; D004331:Drosophila melanogaster / Q000201:enzymology / Q000235:genetics*; D004720:Endonucleases / Q000378:metabolism; D008928:Mitochondria / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis
chemical_list: D018736:DNA Adducts; D029721:Drosophila Proteins; D004720:Endonucleases; D045647:DNA Glycosylases
keywords: Aging; Base excision repair; DNA repair; Drosophila; Microarray; Mitochondria