title: Metal accumulation and oxidative stress biomarkers in liver of freshwater fish Carassius auratus following in vivo exposure to waterborne zinc under different pH values.
abstract: In this study, laboratory experiments were conducted to investigate the combined effect of zinc and pH on metal accumulation and oxidative stress biomarkers in Carassius auratus. Fish were exposed to 0.1 and 1.0mg Zn/L at three pH values (5.0, 7.25, 9.0) for 3, 12, and 30 d. After each exposure, the contents of three trace elements (Zn, Fe and Cu) were determined in liver. Generally, longer exposure to zinc (12d and 30 d) increased hepatic Zn and Cu deposition, but decreased Fe content. Increasing accumulation of Zn in the tissue was also observed with increasing zinc concentration in the exposure medium. Moreover, hepatic antioxidant enzyme activities including superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx), together with the level of glutathione (GSH) were measured to evaluate the oxidative stress status. The decreases in the four measured biochemical parameters after 3d exposure might reflect the failure of the antioxidant defense system in neutralizing the ROS generated during the metabolic process, while the recovery of the antioxidants at days 12 and 30 suggested a possible shift toward a detoxification mechanism. With regard to the influence of pH on zinc toxicity, the general observation was that the living environment became more stressful when the water conditions changed from an acidic state toward a near-neutral or alkaline state.
authors.affiliation: State Key Laboratory of Pollution Control and Resources Reuse, School of Environment, Nanjing University, Nanjing, Jiangsu 210023, PR China.
authors.forename: Ruijuan
authors.initials: R
authors.lastname: Qu
authors.affiliation: State Key Laboratory of Pollution Control and Resources Reuse, School of Environment, Nanjing University, Nanjing, Jiangsu 210023, PR China.
authors.forename: Mingbao
authors.initials: M
authors.lastname: Feng
authors.affiliation: State Key Laboratory of Pollution Control and Resources Reuse, School of Environment, Nanjing University, Nanjing, Jiangsu 210023, PR China.
authors.forename: Xinghao
authors.initials: X
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Pollution Control and Resources Reuse, School of Environment, Nanjing University, Nanjing, Jiangsu 210023, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Qin
authors.affiliation: State Key Laboratory of Pollution Control and Resources Reuse, School of Environment, Nanjing University, Nanjing, Jiangsu 210023, PR China.
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Pollution Control and Resources Reuse, School of Environment, Nanjing University, Nanjing, Jiangsu 210023, PR China. Electronic address: wangzy@nju.edu.cn.
authors.forename: Zunyao
authors.initials: Z
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Pollution Control and Resources Reuse, School of Environment, Nanjing University, Nanjing, Jiangsu 210023, PR China.
authors.forename: Liansheng
authors.initials: L
authors.lastname: Wang
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis; D004781:Environmental Exposure; D005618:Fresh Water; D006054:Goldfish / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000737:chemistry / Q000187:drug effects* / Q000201:enzymology; D008670:Metals / Q000032:analysis / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D010088:Oxidoreductases / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D015032:Zinc / Q000378:metabolism* / Q000633:toxicity*
chemical_list: D015415:Biomarkers; D008670:Metals; D014874:Water Pollutants, Chemical; D010088:Oxidoreductases; D015032:Zinc
keywords: Antioxidant defense; Carassius auratus; Metal content; Zinc; pH