title: Do two different stem cell grafts: G-CSF stimulated and unstimulated bone marrow differ according to hematopoietic colony forming capacity?
abstract: The study was designed to compare colony forming capacity of granulocyte-colony stimulating factor (G-CSF) stimulated bone marrow (G-BM) with standard unstimulated bone marrow (U-BM) of healthy donors of pediatric patients. CFU-Assay results of 26 healthy donors of pediatric patients undergoing allogeneic hematopoietic stem cell transplantation (HSCT) were analyzed retrospectively. 13 of donors received 10 μg/kg per day of G-CSF as a single injection for 3 consecutive days and other 13 of donors had unstimulated BM. Colony forming capacity of hematopoietic stem cells evaluated with Colony Forming Unit-Assay (CFU-Assay) with in semi-solid agar culture medium after 14-18 days of culture period. CFU-Assay results of G-BM and U-BM (expressed as means) were; Burst Forming Unit-Erythroid (BFU-E): 15.20 × 10(4)/kg and 8.38 × 10(4)/kg, Colony Forming Unit-Granulocyte Macrophage (CFU-GM): 10.35 × 10(4)/kg and 5.67 × 10(4)/kg, Colony Forming Unit-Erythroid (CFU-E): 0.59 × 10(4)/kg and 0.33 × 10(4)/kg, CFU-Granulocyte Erythroid Macrophage Megakaryocyte (CFU-GEMM): 0.52 × 10(4)/kg and 0.53 × 10(4)/kg respectively. BFU-E and CFU-GM capacity of G-BM was increased and statistically significantly different than standard U-BM (p ⩽ 0.01). In conclusion, increased colony forming capacity of hematopoietic stem cells of G-BM when compared with standard unstimulated BM could be a major advantage for transplantation.
authors.affiliation: Stem Cell Research Laboratory, Department of Pediatric Hematology Oncology, Ankara Children's Hematology Oncology Education and Research Hospital, Ankara, Turkey; Department of Pediatric Hematology Oncology, Ankara Children's Hematology Oncology Education and Research Hospital, Ankara, Turkey. Electronic address: mozguner@hotmail.com.
authors.forename: Meltem
authors.initials: M
authors.lastname: Özgüner
authors.affiliation: Department of Pediatric Hematology Oncology, Ankara Children's Hematology Oncology Education and Research Hospital, Ankara, Turkey.
authors.forename: Mehmet Fatih
authors.initials: MF
authors.lastname: Azık
authors.affiliation: Department of Pediatric Hematology Oncology, Ankara Children's Hematology Oncology Education and Research Hospital, Ankara, Turkey; Department of Pediatric Hematology, Hacettepe University, Ankara, Turkey.
authors.forename: Betül
authors.initials: B
authors.lastname: Tavil
authors.affiliation: Department of Pediatric Hematology Oncology, Ankara Children's Hematology Oncology Education and Research Hospital, Ankara, Turkey.
authors.forename: Ikbal
authors.initials: I
authors.lastname: Bozkaya
authors.affiliation: Stem Cell Research Laboratory, Department of Pediatric Hematology Oncology, Ankara Children's Hematology Oncology Education and Research Hospital, Ankara, Turkey.
authors.forename: Yasin
authors.initials: Y
authors.lastname: Köksal
authors.affiliation: Stem Cell Research Laboratory, Department of Pediatric Hematology Oncology, Ankara Children's Hematology Oncology Education and Research Hospital, Ankara, Turkey.
authors.forename: Elif
authors.initials: E
authors.lastname: Canal
authors.affiliation: Department of Pediatric Hematology Oncology, Ankara Children's Hematology Oncology Education and Research Hospital, Ankara, Turkey; Department of Pediatric Hematology, Hacettepe University, Ankara, Turkey.
authors.forename: Duygu
authors.initials: D
authors.lastname: Uçkan
authors.affiliation: Department of Pediatric Hematology Oncology, Ankara Children's Hematology Oncology Education and Research Hospital, Ankara, Turkey.
authors.forename: Bahattin
authors.initials: B
authors.lastname: Tunç
mesh_terms: D000293:Adolescent; D000328:Adult; D064591:Allografts; D001853:Bone Marrow / Q000378:metabolism*; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000008:administration & dosage*; D019650:Hematopoietic Stem Cell Mobilization; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D012189:Retrospective Studies; D013997:Time Factors
chemical_list: D016179:Granulocyte Colony-Stimulating Factor
keywords: CFU-Assay; Hematopoietic stem cells