title: Adenovirus-mediated CCL20/IL-15 gene transfer enhances antitumor immunity in mice.
abstract: The CCL20 chemokine has potent antitumor activities through chemoattracting immature dentritic cells. But the maturation status of tumoral dentritic cells is important limiting factors in DC-based immunity. The endogenous availability of IL-15 was effective in inducing the dentritic cells maturation and IL-15 also shows tumor-specific antitumor activities. We constructed a CCL20/IL-15 bicistronic adenovirus (Ad-CCL20-IL-15) and confirmed its combined antitumor effect in vitro and in vivo. Intratumoral injection of Ad-CCL20-IL-15 into both CT-26 and B16F10 cells resulted in marked reduction of tumor growth in our model. Splenocytes treated by Ad-CCL20-IL-15 developed tumor-specific cytotoxic T cells and IFN-γ secretion could protect mice from rechallenging. This study suggests that CCL20/IL-15 can induce a strong antitumor immune response in tumor tissues and it is a suitable candidate for cancer immunotherapy.
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, China-Japan Union Hospital, Jilin University, Changchun 130062, PR China.
authors.forename: Guang-yao
authors.initials: GY
authors.lastname: Liu
authors.affiliation: Affiliated Hospital of Changchun University of Traditional Chinese Medicine, Changchun 130033, PR China.
authors.forename: Qiu-ying
authors.initials: QY
authors.lastname: Jia
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, PR China.
authors.forename: Zhi-jie
authors.initials: ZJ
authors.lastname: Li
authors.affiliation: Fourth Hospital of Jilin University, Changchun 130062, PR China.
authors.forename: Ya-gang
authors.initials: YG
authors.lastname: Li
authors.affiliation: Fourth Hospital of Jilin University, Changchun 130062, PR China.
authors.forename: Meng-meng
authors.initials: MM
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, China-Japan Union Hospital, Jilin University, Changchun 130062, PR China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, PR China.
authors.forename: Ming-yuan
authors.initials: MY
authors.lastname: Liu
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, China-Japan Union Hospital, Jilin University, Changchun 130062, PR China.
authors.forename: Xue-lin
authors.initials: XL
authors.lastname: Wang
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, China-Japan Union Hospital, Jilin University, Changchun 130062, PR China. Electronic address: myth0317@yeah.net.
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement; D054418:Chemokine CCL20 / Q000235:genetics*; D003713:Dendritic Cells / Q000276:immunology*; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000235:genetics / Q000627:therapeutic use*; D006801:Humans; D007167:Immunotherapy; D007371:Interferon-gamma / Q000276:immunology / Q000378:metabolism; D019409:Interleukin-15 / Q000235:genetics*; D007694:Killer Cells, Natural / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009369:Neoplasms / Q000276:immunology / Q000628:therapy; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology
chemical_list: C493447:CCL20 protein, mouse; D054418:Chemokine CCL20; D019409:Interleukin-15; D007371:Interferon-gamma
keywords: Bicistronic adenovirus; CCL20; Cancer immunotherapy; Chemokines; IL-15