title: Effects of polydopamine functionalized titanium dioxide nanotubes on endothelial cell and smooth muscle cell.
abstract: Previous investigations have demonstrated that TiO2 nanotubes (NTs) with particular structure cues could control the behavior of different types of cells, including endothelial cells (ECs) and smooth muscle cells (SMCs). Besides, polydopamine (PDA) modified surfaces were reported to be beneficial to increase the proliferation and viability of ECs and meanwhile could inhibit the proliferation of SMCs. The TiO2 nanotubes (NTs) were functionalized with polydopamine (PDA) (PDA/NTs) to study the synergetic effect of both nanotopography (NTs) and chemical cues (PDA) of TiO2 nanotubes on the regulation of cellular behavior of ECs and SMCs. The PDA-modified TiO2 nanotubes were subjected to field-emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS), and water contact angle (WCA) analysis. In vitro cell culture tests confirmed that, comparing with flat titanium (Ti) and TiO2 nanotubes, PDA/NTs surface synergistically promoted ECs attachment, proliferation, migration and release of nitric oxide (NO). Meanwhile, the PDA/NTs performed well in reducing SMCs adhesion and proliferation. This novel approach might provide a new platform to investigate the synergistic effect of local chemistry and topography, as well as the applications for the development of titanium-based implants for enhanced endothelialization.
authors.affiliation: School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China; Key Lab of Advanced Technology of Materials of Education Ministry, Southwest of Jiaotong University, Chengdu 610031, China.
authors.forename: Si
authors.initials: S
authors.lastname: Zhong
authors.affiliation: School of Material Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China; Key Lab of Advanced Technology of Materials of Education Ministry, Southwest of Jiaotong University, Chengdu 610031, China. Electronic address: lrifang@126.com.
authors.forename: Rifang
authors.initials: R
authors.lastname: Luo
authors.affiliation: School of Material Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China; Key Lab of Advanced Technology of Materials of Education Ministry, Southwest of Jiaotong University, Chengdu 610031, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: School of Material Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China; Key Lab of Advanced Technology of Materials of Education Ministry, Southwest of Jiaotong University, Chengdu 610031, China.
authors.forename: Linlin
authors.initials: L
authors.lastname: Tang
authors.affiliation: School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China. Electronic address: Wujianeer@sina.com.
authors.forename: Jian
authors.initials: J
authors.lastname: Wu
authors.affiliation: School of Material Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China; Key Lab of Advanced Technology of Materials of Education Ministry, Southwest of Jiaotong University, Chengdu 610031, China. Electronic address: jinxxwang@263.net.
authors.forename: Jin
authors.initials: J
authors.lastname: Wang
authors.affiliation: School of Manufacturing Science and Engineering, Sichuan University, Chengdu 610065, China.
authors.forename: Runbo
authors.initials: R
authors.lastname: Huang
authors.affiliation: School of Material Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China; Key Lab of Advanced Technology of Materials of Education Ministry, Southwest of Jiaotong University, Chengdu 610031, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Sun
authors.affiliation: School of Material Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China; Key Lab of Advanced Technology of Materials of Education Ministry, Southwest of Jiaotong University, Chengdu 610031, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Huang
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D007211:Indoles / Q000737:chemistry / Q000494:pharmacology*; D032389:Myocytes, Smooth Muscle / Q000187:drug effects* / Q000378:metabolism; D043942:Nanotubes / Q000737:chemistry*; D010316:Particle Size; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D007211:Indoles; D011108:Polymers; C568283:polydopamine; C009495:titanium dioxide; D014025:Titanium
keywords: Endothelialization; Polydopamine; Smooth muscle cell (SMC); Surface modification; TiO(2) nanotubes; Vascular stent