title: Associated analysis of single nucleotide polymorphisms found on exon 3 of the IGF-1 gene with Tibetan miniature pig growth traits.
abstract: The IGF-1 gene is an important regulating factor that has a growth-promoting effect on growth hormone. The IGF-1 gene promotes muscle cell differentiation in the muscle cell formation process. The IGF-1 gene also regulates the growth of skeletal muscle during skeletal muscle growth. In addition, the IGF-1 gene plays an important role in the formation of mammals and poultry embryos, and the process of postnatal growth. The IGF-1 gene has been implicated as a candidate gene for the regulation of pig growth traits. We analyzed exon 3 of the IGF-1 gene polymorphism in Tibetan miniature pigs (N = 128) by polymerase chain reaction-single-strand conformation polymorphism and DNA sequencing. One single nucleotide polymorphism (T40C) was found on exon 3 of the IGF-1 gene. Statistical analysis of genotype frequencies revealed that the T allele was dominant in Tibetan miniature pigs at the T40C locus. The association analysis showed that the IGF-1 mutation had an effect on the body weight, body length, and chest circumference of pigs aged 6-8 months. In addition, the IGF-1 mutation had an effect on body weight in pigs aged 9-11 months (P < 0.05). We speculated that the pigs with the TT genotype grow more rapidly compared to those with the TC genotype. The TC genotype of the Tibetan miniature pig has a smaller body type. This information provides a theoretical basis for the genetic background of Tibetan miniature pigs.
authors.affiliation: Department of Laboratory Animal Center, Southern Medical University, Guangzhou, China.
authors.forename: M
authors.initials: M
authors.lastname: Yue
authors.affiliation: Department of Laboratory Animal Center, Southern Medical University, Guangzhou, China.
authors.forename: Y G
authors.initials: YG
authors.lastname: Tian
authors.affiliation: Department of Laboratory Animal Center, Southern Medical University, Guangzhou, China.
authors.forename: Y J
authors.initials: YJ
authors.lastname: Wang
authors.affiliation: Department of Laboratory Animal Center, Southern Medical University, Guangzhou, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Gu
authors.affiliation: Songshan Lake Pearl Laboratory Animal Science & Technology Co., Ltd., Dongguan, China.
authors.forename: N
authors.initials: N
authors.lastname: Bayaer
authors.affiliation: Songshan Lake Pearl Laboratory Animal Science & Technology Co., Ltd., Dongguan, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Hu
authors.affiliation: Department of Laboratory Animal Center, Southern Medical University, Guangzhou, China weiwanggu@126.com.
authors.forename: W W
authors.initials: WW
authors.lastname: Gu
mesh_terms: D000818:Animals; D049628:Body Size; D001947:Breeding; D005091:Exons; D056726:Genetic Association Studies; D005838:Genotype; D007334:Insulin-Like Growth Factor I / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D013045:Species Specificity; D013552:Swine; D013556:Swine, Miniature / Q000235:genetics* / Q000254:growth & development*; D018609:Tibet
chemical_list: D007334:Insulin-Like Growth Factor I