title: Modulation of imprinted gene expression following superovulation.
abstract: Although assisted reproductive technologies increase the risk of low birth weight and genomic imprinting disorders, the precise underlying causes remain unclear. Using a mouse model, we previously showed that superovulation alters the expression of imprinted genes in the placenta at 9.5days (E9.5) of gestation. Here, we investigate whether effects of superovulation on genomic imprinting persisted at later stages of development and assess the surviving fetuses for growth and morphological abnormalities. Superovulation, followed by embryo transfer at E3.5, as compared to spontaneous ovulation (controls), resulted in embryos of normal size and weight at 14.5 and 18.5days of gestation. The normal monoallelic expression of the imprinted genes H19, Snrpn and Kcnq1ot1 was unaffected in either the placentae or the embryos from the superovulated females at E14.5 or E18.5. However, for the paternally expressed imprinted gene Igf2, superovulation generated placentae with reduced production of the mature protein at E9.5 and significantly more variable mRNA levels at E14.5. We propose that superovulation results in the ovulation of abnormal oocytes with altered expression of imprinted genes, but that the coregulated genes of the imprinted gene network result in modulated expression.
authors.affiliation: Research Institute at the Montreal Children's Hospital of the McGill University Health Centre and Department of Human Genetics, McGill University, Montreal, Quebec H3H 1P3, Canada.
authors.forename: Amanda L
authors.initials: AL
authors.lastname: Fortier
authors.affiliation: Research Institute at the Montreal Children's Hospital of the McGill University Health Centre and Department of Human Genetics, McGill University, Montreal, Quebec H3H 1P3, Canada; Research Institute at the Montreal Children's Hospital of the McGill University Health Centre and Department of Pediatrics, McGill University, Montreal, Quebec H3H 1P3, Canada.
authors.forename: Serge
authors.initials: S
authors.lastname: McGraw
authors.affiliation: Research Institute at the Montreal Children's Hospital of the McGill University Health Centre and Department of Human Genetics, McGill University, Montreal, Quebec H3H 1P3, Canada; Research Institute at the Montreal Children's Hospital of the McGill University Health Centre and Department of Pediatrics, McGill University, Montreal, Quebec H3H 1P3, Canada.
authors.forename: Flavia L
authors.initials: FL
authors.lastname: Lopes
authors.affiliation: Research Institute at the Montreal Children's Hospital of the McGill University Health Centre and Department of Human Genetics, McGill University, Montreal, Quebec H3H 1P3, Canada.
authors.forename: Kirsten M
authors.initials: KM
authors.lastname: Niles
authors.affiliation: Research Institute at the Montreal Children's Hospital of the McGill University Health Centre and Department of Pediatrics, McGill University, Montreal, Quebec H3H 1P3, Canada.
authors.forename: Mylène
authors.initials: M
authors.lastname: Landry
authors.affiliation: Research Institute at the Montreal Children's Hospital of the McGill University Health Centre and Department of Human Genetics, McGill University, Montreal, Quebec H3H 1P3, Canada; Research Institute at the Montreal Children's Hospital of the McGill University Health Centre and Department of Pediatrics, McGill University, Montreal, Quebec H3H 1P3, Canada; Research Institute at the Montreal Children's Hospital of the McGill University Health Centre and Department of Pharmacology and Therapeutics, McGill University, Montreal, Quebec H3H 1P3, Canada. Electronic address: jacquetta.trasler@mcgill.ca.
authors.forename: Jacquetta M
authors.initials: JM
authors.lastname: Trasler
mesh_terms: D000818:Animals; D019175:DNA Methylation; D004622:Embryo, Mammalian / Q000378:metabolism; D044127:Epigenesis, Genetic; D005260:Female; D018392:Genomic Imprinting; D007335:Insulin-Like Growth Factor II / Q000235:genetics / Q000378:metabolism; D051379:Mice; D009929:Organ Size; D010920:Placenta / Q000378:metabolism; D010929:Placentation; D011247:Pregnancy; D011256:Pregnancy Outcome; D062085:RNA, Long Noncoding / Q000235:genetics; D013480:Superovulation / Q000235:genetics / Q000378:metabolism*
chemical_list: C094335:H19 long non-coding RNA; C513121:IGF2 protein, mouse; D062085:RNA, Long Noncoding; D007335:Insulin-Like Growth Factor II
keywords: H19; Igf2; Imprinting; Mouse; Placenta; Superovulation