title: Excess iron undermined bone load-bearing capacity through tumor necrosis factor-α-dependent osteoclastic activation in mice.
abstract: Iron overload has been associated with bone mass loss. To elucidate the effects of excess iron on bone metabolism, an iron-overloading mouse model was established by administering iron-dextran at 250 mg/kg to female BALB/c mice. After 4 weeks, the mice were sacrificed and the biomechanical properties of the femurs were examined. The results suggested a notable decrease of the maximal bending stress and the modulus of bending elasticity in the femurs obtained from the excess iron-treated mice compared to the control mice. The levels of the serum osteocalcin, C-telopeptide of type I collagen (CTX-1) and tumor necrosis factor-α (TNF-α) were measured in order to investigate the underlying mechanism responsible for the excess iron-induced bone strength reduction. Overall, the results suggested that iron overload resulted in a marked reduction of bone load-bearing capacity through a TNF-triggered osteoclast differentiation and resorption mechanism.
authors.affiliation: Shandong Institute of Endocrine and Metabolic Diseases, Jinan 250062; ; State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, P.R. China.
authors.forename: Junping
authors.initials: J
authors.lastname: Li
authors.affiliation: Shandong Institute of Endocrine and Metabolic Diseases, Jinan 250062; ; State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, P.R. China.
authors.forename: Yanli
authors.initials: Y
authors.lastname: Hou
authors.affiliation: State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, P.R. China.
authors.forename: Shuping
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Shandong Institute of Endocrine and Metabolic Diseases, Jinan 250062;
authors.forename: Hong
authors.initials: H
authors.lastname: Ji
authors.affiliation: Shandong Institute of Endocrine and Metabolic Diseases, Jinan 250062;
authors.forename: Haiqin
authors.initials: H
authors.lastname: Rong
authors.affiliation: State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, P.R. China.
authors.forename: Guangbo
authors.initials: G
authors.lastname: Qu
authors.affiliation: State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, P.R. China.
authors.forename: Sijin
authors.initials: S
authors.lastname: Liu
keywords: bone mass; bone resorption; bone strength; iron overload