title: Polyacetylenes from Radix et Rhizoma Notopterygii incisi with an inhibitory effect on nitric oxide production in vitro.
abstract: Notopterygium roots (Qiang Huo) have been used in traditional Chinese medicine for treating colds, inflammatory diseases like rheumatoid arthritis, and as an analgesic. The anti-inflammatory activity of the roots of Notopterygium incisum has been evaluated by testing the inhibitory activity on nitric oxide production by inducible nitric oxide synthase. The apparent authenticity of the sample was checked by DNA sequence comparison. Using activity-guided isolation, different compounds were isolated and structurally characterized by means of NMR and mass spectroscopy. Eight polyacetylenes could be identified and were tested on their inhibitory activity on nitric oxide production in RAW 264.7 mouse macrophages using the Griess assay. Different 3-hydroxy allyl polyacetylenes exhibited significant activity (IC50: 8-acetoxyfalcarinol, 20.1 µM; falcarindiol, 9.2 µM; 9-epoxyfalcarindiol, 8.8 µM; and crithmumdiol, 23.6 µM).
authors.affiliation: Institute of Pharmaceutical Sciences, Department of Pharmacognosy, University of Graz, Austria.
authors.forename: Martina
authors.initials: M
authors.lastname: Blunder
authors.affiliation: Institute of Pharmaceutical Sciences, Department of Pharmacognosy, University of Graz, Austria.
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.affiliation: Institute of Pharmaceutical Sciences, Department of Pharmaceutical Chemistry, University of Graz, Austria.
authors.forename: Olaf
authors.initials: O
authors.lastname: Kunert
authors.affiliation: Institute of Pharmaceutical Sciences, Department of Pharmacognosy, University of Graz, Austria.
authors.forename: Nora Anna
authors.initials: NA
authors.lastname: Winkler
authors.affiliation: Institute of Pharmaceutical Sciences, Department of Pharmacognosy, University of Graz, Austria.
authors.forename: Andreas
authors.initials: A
authors.lastname: Schinkovitz
authors.affiliation: Institute of Animal Nutrition an Functional Plant Compounds, University of Veterinary Medicine, Vienna, Austria.
authors.forename: Corinna
authors.initials: C
authors.lastname: Schmiderer
authors.affiliation: Institute of Animal Nutrition an Functional Plant Compounds, University of Veterinary Medicine, Vienna, Austria.
authors.forename: Johannes
authors.initials: J
authors.lastname: Novak
authors.affiliation: Institute of Pharmaceutical Sciences, Department of Pharmacognosy, University of Graz, Austria.
authors.forename: Rudolf
authors.initials: R
authors.lastname: Bauer
mesh_terms: D000818:Animals; D019661:Apiaceae / Q000737:chemistry*; D002460:Cell Line; D066298:In Vitro Techniques; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D051379:Mice; D008969:Molecular Sequence Data; D009569:Nitric Oxide / Q000096:biosynthesis*; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D018517:Plant Roots / Q000737:chemistry*; D053279:Polyynes / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D053279:Polyynes; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II