title: Domains of the cucumber mosaic virus 2b silencing suppressor protein affecting inhibition of salicylic acid-induced resistance and priming of salicylic acid accumulation during infection.
abstract: The cucumber mosaic virus (CMV) 2b silencing suppressor protein allows the virus to overcome resistance to replication and local movement in inoculated leaves of plants treated with salicylic acid (SA), a resistance-inducing plant hormone. In Arabidopsis thaliana plants systemically infected with CMV, the 2b protein also primes the induction of SA biosynthesis during this compatible interaction. We found that CMV infection of susceptible tobacco (Nicotiana tabacum) also induced SA accumulation. Utilization of mutant 2b proteins expressed during infection of tobacco showed that the N- and C-terminal domains, which had previously been implicated in regulation of symptom induction, were both required for subversion of SA-induced resistance, while all mutants tested except those affecting the putative phosphorylation domain had lost the ability to prime SA accumulation and expression of the SA-induced marker gene PR-1.
authors.affiliation: Department of Plant Pathology, China Agricultural University, 2 Yuanmingyuan West Rd, Beijing 100193, PR China.|Department of Plant Sciences, University of Cambridge, Downing Street, Cambridge CB2 3EA, UK.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhou
authors.affiliation: Department of Plant Sciences, University of Cambridge, Downing Street, Cambridge CB2 3EA, UK.
authors.forename: Alex M
authors.initials: AM
authors.lastname: Murphy
authors.affiliation: Department of Plant Sciences, University of Cambridge, Downing Street, Cambridge CB2 3EA, UK.
authors.forename: Mathew G
authors.initials: MG
authors.lastname: Lewsey
authors.affiliation: Department of Plant Sciences, University of Cambridge, Downing Street, Cambridge CB2 3EA, UK.
authors.forename: Jack H
authors.initials: JH
authors.lastname: Westwood
authors.affiliation: State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, 198 Shiqiao Rd, Hangzhou 310021, Zhejiang, PR China.|Department of Plant Sciences, University of Cambridge, Downing Street, Cambridge CB2 3EA, UK.
authors.forename: Heng-Mu
authors.initials: HM
authors.lastname: Zhang
authors.affiliation: Centro de Investigaciones Biológicas, CIB, CSIC, Ramiro de Maeztu 9, 28040 Madrid, Spain.|Department of Plant Sciences, University of Cambridge, Downing Street, Cambridge CB2 3EA, UK.
authors.forename: Inmaculada
authors.initials: I
authors.lastname: González
authors.affiliation: Centro de Investigaciones Biológicas, CIB, CSIC, Ramiro de Maeztu 9, 28040 Madrid, Spain.
authors.forename: Tomás
authors.initials: T
authors.lastname: Canto
authors.affiliation: Department of Plant Sciences, University of Cambridge, Downing Street, Cambridge CB2 3EA, UK.
authors.forename: John P
authors.initials: JP
authors.lastname: Carr
mesh_terms: D017360:Arabidopsis / Q000378:metabolism / Q000821:virology; D017799:Cucumovirus / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity; D005814:Genes, Viral; D009154:Mutation; D010935:Plant Diseases / Q000821:virology; D010937:Plant Growth Regulators / Q000378:metabolism; D018515:Plant Leaves / Q000378:metabolism / Q000821:virology; D030821:Plants, Genetically Modified; D017434:Protein Structure, Tertiary; D034622:RNA Interference; D020156:Salicylic Acid / Q000378:metabolism*; D014026:Nicotiana / Q000378:metabolism / Q000821:virology; D014764:Viral Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
chemical_list: C404227:2b protein, cucumber mosaic virus; D010937:Plant Growth Regulators; D014764:Viral Proteins; D020156:Salicylic Acid