title: Rapid isolation of a facultative anaerobic electrochemically active bacterium capable of oxidizing acetate for electrogenesis and azo dyes reduction.
abstract: In this study, 27 strains of electrochemically active bacteria (EAB) were rapidly isolated and their capabilities of extracellular electron transfer were identified using a photometric method based on WO3 nanoclusters. These strains caused color change of WO3 from white to blue in a 24-well agar plate within 40 h. Most of the isolated EAB strains belonged to the genera of Aeromonas and Shewanella. One isolate, Pantoea agglomerans S5-44, was identified as an EAB that can utilize acetate as the carbon source to produce electricity and reduce azo dyes under anaerobic conditions. The results confirmed the capability of P. agglomerans S5-44 for extracellular electron transfer. The isolation of this acetate-utilizing, facultative EBA reveals the metabolic diversity of environmental bacteria. Such strains have great potential for environmental applications, especially at interfaces of aerobic and anaerobic environments, where acetate is the main available carbon source.
authors.affiliation: Department of Chemistry, University of Science & Technology of China, Hefei, 230026, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Shen
authors.forename: Shi-Jie
authors.initials: SJ
authors.lastname: Yuan
authors.forename: Chao
authors.initials: C
authors.lastname: Wu
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Cheng
authors.forename: Xiang-Ning
authors.initials: XN
authors.lastname: Song
authors.forename: Wen-Wei
authors.initials: WW
authors.lastname: Li
authors.forename: Zhong-Hua
authors.initials: ZH
authors.lastname: Tong
authors.forename: Han-Qing
authors.initials: HQ
authors.lastname: Yu
mesh_terms: D000085:Acetates / Q000378:metabolism*; D001391:Azo Compounds / Q000737:chemistry / Q000378:metabolism*; D001421:Bacteria, Anaerobic / Q000145:classification / Q000166:cytology / Q000302:isolation & purification* / Q000378:metabolism*; D001674:Bioelectric Energy Sources / Q000382:microbiology*; D004396:Coloring Agents / Q000378:metabolism*; D004563:Electrochemistry; D004579:Electron Transport; D019015:Geologic Sediments / Q000382:microbiology; D053758:Nanoparticles / Q000737:chemistry; D010087:Oxides / Q000737:chemistry; D010802:Phylogeny; D013997:Time Factors; D014414:Tungsten / Q000737:chemistry
chemical_list: D000085:Acetates; D001391:Azo Compounds; D004396:Coloring Agents; D010087:Oxides; C100258:methyl orange; C511604:tungsten oxide; D014414:Tungsten