title: Transcriptional profiling of erythroid progenitors from G-CSF mobilized and nonmobilized peripheral blood.
abstract: OBJECTIVE
  The purpose of this study was to examine the gene expression profile of granulocyte colony stimulating factor (G-CSF)-mobilized peripheral blood (mPB)-derived progenitors, used in transplantation.
  
  
  METHODS
  We correlated gene expression patterns of highly enriched steady-state peripheral blood (PB)- and mPB-derived CD71+ cells by microarray and ingenuity pathway analyses, to identify the transcriptional program during in vitro erythroid differentiation.
  
  
  RESULTS
  The gene expression was more than doubled in mPB-derived (4180 genes) compared to PB-derived erythroid progenitors (1667 genes) while PB-and mPB-derived erythroid progenitors shared 1534 common genes. Comparative analysis of transcript levels showed differential expression of 54 genes between cultured erythroid progenitors of PB and mPB origin, where we identified common 13 downregulated and 30 upregulated genes. The most significant genes in mPB-derived erythroid progenitors were P4HB, DDIA3, ARPC2 and ATP5G3. Regarding G-CSF stimulation the G-CSF receptor CSF2RB (1.1-fold) was linked via STAT3 to erythroid-specific ALAS2 (2.9-fold) and GATA2 (1.3-fold) factors, all upregulated in mPB-derived erythroid progenitors, coupled to common upregulated NUDC gene involved in the proliferation of erythroid cells.
  
  
  CONCLUSIONS
  This report provides an extensive transcriptional profile of cultured erythroid progenitors and leads to a better understanding of diversity among the progenitor sources.
authors.affiliation: Laboratory of Experimental Hematology, Institute for Medical Research, University of Belgrade, Belgrade, Serbia.
authors.forename: Vladan P
authors.initials: VP
authors.lastname: Cokic
authors.forename: Milos
authors.initials: M
authors.lastname: Diklic
authors.forename: Tijana
authors.initials: T
authors.lastname: Suboticki
authors.forename: Bojana B
authors.initials: BB
authors.lastname: Beleslin-Cokic
authors.forename: Dragana
authors.initials: D
authors.lastname: Markovic
authors.forename: Pavle
authors.initials: P
authors.lastname: Milenkovic
authors.forename: Gordana
authors.initials: G
authors.lastname: Jovcic
mesh_terms: D002454:Cell Differentiation / Q000235:genetics; D002478:Cells, Cultured; D015672:Erythroid Precursor Cells / Q000378:metabolism* / Q000637:transplantation; D053263:Gene Regulatory Networks / Q000235:genetics; D016179:Granulocyte Colony-Stimulating Factor / Q000378:metabolism* / Q000494:pharmacology; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D016188:Receptors, Granulocyte Colony-Stimulating Factor / Q000378:metabolism*; D046888:Tissue Array Analysis; D059467:Transcriptome
chemical_list: D016188:Receptors, Granulocyte Colony-Stimulating Factor; D016179:Granulocyte Colony-Stimulating Factor