title: Identification of RNF114 as a novel positive regulatory protein for T cell activation.
abstract: RNF114 [RING (really interesting new gene) finger protein 114] has been shown to be a novel psoriasis susceptibility gene, with a putative role in the regulation of immune responses, though the underlying mechanism was not fully identified. In the present study, to investigate whether RNF114 is involved in T cell activation, a series of fluorescence activated cell sortings (FACS) were performed. The analysis confirmed that RNF114 over-expression had a promotion effect on T cell activation with an average 43.97% increment and the upregulatory roles showed a dose-dependent effect with 18.44% increment. Interestingly, the two C2H2 domains were shown to play important but opposite roles in T cell activation. The deficiency of upstream C2H2 domain increased the efficiency of T cell activation by 12.81%, while the downstream C2H2 domain alone promoted it with an average level 25.12% higher than intact RNF114 protein. Combined with tandem affinity purification (TAP) and mass spectrometry, our investigations found 23 RNF114-interacting proteins that have distinct physiological roles in transcription, translation, DNA repair and signaling pathways. These findings, including recognition of RNF114 as a positive regulatory protein and identification of its interacting proteins, widen the understanding for investigating functions of RNF114 involved in T cell activation.
authors.affiliation: Department of Medical Genetics and Division of Morbid Genomics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, PR China; Department of Biomedicine, Chengdu Medical College, Chengdu, PR China.
authors.forename: Ping
authors.initials: P
authors.lastname: Yang
authors.affiliation: Department of Medical Genetics and Division of Morbid Genomics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, PR China.
authors.forename: Yilu
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Center of Science and Research, Chengdu Medical College, Chengdu, PR China.
authors.forename: Minhui
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Medical Genetics and Division of Morbid Genomics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, PR China.
authors.forename: Kun
authors.initials: K
authors.lastname: Zhang
authors.affiliation: Department of Medical Genetics and Division of Morbid Genomics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, PR China.
authors.forename: Chao
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Medical Genetics and Division of Morbid Genomics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, PR China.
authors.forename: Huijuan
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Medical Genetics and Division of Morbid Genomics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, PR China.
authors.forename: Dachang
authors.initials: D
authors.lastname: Tao
authors.affiliation: Department of Medical Genetics and Division of Morbid Genomics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, PR China.
authors.forename: Sizhong
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Medical Genetics and Division of Morbid Genomics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, PR China. Electronic address: mayongxin@gmail.com.
authors.forename: Yongxin
authors.initials: Y
authors.lastname: Ma
mesh_terms: D002352:Carrier Proteins / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology*; D002460:Cell Line; D005786:Gene Expression Regulation; D049452:Green Fluorescent Proteins / Q000235:genetics; D006801:Humans; D019169:Jurkat Cells; D008213:Lymphocyte Activation / Q000276:immunology*; D017434:Protein Structure, Tertiary; D011565:Psoriasis / Q000235:genetics; D011994:Recombinant Proteins / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000276:immunology*; D044767:Ubiquitin-Protein Ligases
chemical_list: D002352:Carrier Proteins; D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins; C477146:RNF114 protein, human; D044767:Ubiquitin-Protein Ligases
keywords: Domain; RING; RNF114; Regulator; T cell activation