title: Preparation of petroleum-degrading bacterial agent and its application in remediation of contaminated soil in Shengli Oil Field, China.
abstract: Two petroleum-degrading strains were screened from oil fields and denoted as SWH-1 (Bacillus subtilis) and SWH-2 (Sphingobacterium multivorum), which were used to ferment and prepare bacterial agent to remediate petroleum-contaminated sites in Shengli Oil Field in China. The optimal liquid fermentation medium and conditions were MgSO₄·7H₂O (0.5%), NaCl (0.5%), soybean dregs (3%), pH 7.0, culturing at 30 °C, and 220 r/min for 16 h. Peat was chosen as the bacterial carrier due to its ability of keeping microbial activity. Mixed fermented liquid was added into peat (1:2) and air-dried, and the bacterial agent was obtained. It was applied to the petroleum-contaminated soil, which was irrigated, tilled, and fertilized. The removal rate reached 67.7% after 2 months of remediation. During remediation, the quantity of indigenous bacteria varied a lot, while the inoculated bacteria remained stable; the dehydrogenase activity was at high levels and then decreased. Indigenous microorganisms, inoculated bacterial agent, nutrients, water, and soil permeability all played important roles. The study prepared an environment-friendly bacterial agent and established a set of bioremediation technique, which provided further insights into integration of fermentation engineering and soil remediation engineering.
authors.affiliation: School of Life Science, Shandong University, Jinan, 250100, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yu
authors.forename: Wen
authors.initials: W
authors.lastname: Zhang
authors.forename: Guanhong
authors.initials: G
authors.lastname: Chen
authors.forename: Yongchao
authors.initials: Y
authors.lastname: Gao
authors.forename: Jianing
authors.initials: J
authors.lastname: Wang
mesh_terms: D001412:Bacillus subtilis / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002681:China; D004365:Drugs, Chinese Herbal; D061329:Oil and Gas Fields; D010578:Petroleum / Q000032:analysis; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000737:chemistry*; D045249:Sphingobacterium / Q000378:metabolism*
chemical_list: D004365:Drugs, Chinese Herbal; D010578:Petroleum; D012989:Soil Pollutants; C532630:shengli