title: Antioxidative and antiproliferative evaluation of 2-(phenylselenomethyl)tetrahydrofuran and 2-(phenylselenomethyl)tetrahydropyran.
abstract: OBJECTIVE
  To determine the antioxidant and antiproliferative influence of 2-(phenylselenomethyl)tetrahydrofuran (1a) and 2-(phenylselenomethyl)tetrahydropyran (2a) on colon cancer cell line HCT-116 and breast cancer cell line MDA-MB-231.
  
  
  METHODS
  Cell viability was monitored in a dose-dependent manner using MTT assay. The concentration of superoxide anion radical (O2 •(-)) was determined spectrophotometrically. Spectrophotometric determination of nitrites (NO2 -) was performed by using the Griess method. Determination of total glutathione (GSH) was also performed spectrophotometrically.
  
  
  RESULTS
  HCT-116 cell line was more sensitive to the effects of the investigated substances than MDA-MB-231 cell line. Also, it was noticed that 1a produced greater effect compared to 2a. Moreover, both investigated compounds decreased to a certain degree the oxidative stress by decreasing the O2•(-) and thus the peroxynitrite concentration. At the same time, 1a and 2a acted more efficiently in promoting the endogenous antioxidative capacities (increased GSH concentration) providing better self-defence capabilities for cells.
  
  
  CONCLUSIONS
  Our findings showed that the investigated selenium compounds play an important role in reducing the levels of reactive oxygen species (ROS); therefore, we believe that, as antioxidants, they could prevent the processes arising as a consequence of oxidative stress, including cancer.
authors.affiliation: Department for Biology and Ecology, Faculty of Science, University of Kragujevac, Kragujevac, Serbia.
authors.forename: Jelena V
authors.initials: JV
authors.lastname: Kosaric
authors.forename: Danijela M
authors.initials: DM
authors.lastname: Cvetkovic
authors.forename: Marko N
authors.initials: MN
authors.lastname: Zivanovic
authors.forename: Milena G
authors.initials: MG
authors.lastname: Curcic
authors.forename: Dragana S
authors.initials: DS
authors.lastname: Seklic
authors.forename: Zorica M
authors.initials: ZM
authors.lastname: Bugarcic
authors.forename: Snezana D
authors.initials: SD
authors.lastname: Markovic
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D005663:Furans / Q000494:pharmacology*; D045325:HCT116 Cells; D006801:Humans; D016566:Organoselenium Compounds / Q000627:therapeutic use*; D018384:Oxidative Stress / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism*
chemical_list: C588307:2-(phenylselenomethyl)tetrahydrofuran; D000975:Antioxidants; D005663:Furans; D016566:Organoselenium Compounds; D017382:Reactive Oxygen Species; D013481:Superoxides; C018674:tetrahydrofuran