title: Glycan array analysis of influenza H1N1 binding and release.
abstract: Influenza viruses initiate infection by attaching to sialic acid receptors on the surface of host cells. It has been recognized for some time that avian influenza viruses usually bind to terminal sialic acid that is linked in the α2-3 configuration to the next sugar while human viruses show preference for α2-6 linked sialic acid. With developments in synthetic chemistry and chemo-enzymatic methods of synthesizing quite complex glycans, it has become clear that the binding specificity extends beyond the sialic acid, and this has led to considerable interest in developing glycan reagents that could be used either as a diagnostic tool for particular influenza viruses, or to identify cells that are susceptible to infection by certain influenza viruses. Here we describe the use of the Consortium for Functional Glycomics Glycan Array to investigate binding specificity of influenza hemagglutinin and cleavage by neuraminidase, using seasonal and pandemic H1N1 influenza viruses as examples, and compare the results with published data using other array methods.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, OK, USA.
authors.forename: Shelly
authors.initials: S
authors.lastname: Gulati
authors.affiliation: Department of Biochemistry, Emory University, OK, USA.
authors.forename: Yi
authors.initials: Y
authors.lastname: Lasanajak
authors.affiliation: Department of Biochemistry, Emory University, OK, USA.
authors.forename: David F
authors.initials: DF
authors.lastname: Smith
authors.affiliation: Department of Biochemistry, Emory University, OK, USA.
authors.forename: Richard D
authors.initials: RD
authors.lastname: Cummings
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, OK, USA.
authors.forename: Gillian M
authors.initials: GM
authors.lastname: Air
mesh_terms: D000818:Animals; D002642:Chick Embryo; D004285:Dogs; D006388:Hemagglutinins / Q000378:metabolism*; D006801:Humans; D006834:Hydrazines / Q000737:chemistry; D053118:Influenza A Virus, H1N1 Subtype / Q000378:metabolism*; D007251:Influenza, Human / Q000821:virology; D061985:Madin Darby Canine Kidney Cells; D009439:Neuraminidase / Q000378:metabolism*; D011134:Polysaccharides / Q000032:analysis / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism; D053585:Virus Attachment
chemical_list: C417561:Alexa 488 hydrazide; D006388:Hemagglutinins; D006834:Hydrazines; D011134:Polysaccharides; D011956:Receptors, Cell Surface; C092488:sialic acid receptor; D009439:Neuraminidase
keywords: Consortium for Functional Glycomics; Glycan array; Influenza virus; hemagglutinin; neuraminidase