title: Development of a serology-based assay for efficacy evaluation of a lactococcicosis vaccine in Seriola fish.
abstract: Lactococcicosis is an infection caused by the bacterium Lactococcus garvieae and creates serious economic damage to cultured marine and fresh water fish industries. The use of the assay currently applied to evaluate the potency of the lactococcicosis vaccine is contingent upon meeting specific parameters after statistical analysis of the percent survival of the vaccinated yellowtail or greater amberjack fish after challenge with a virulent strain of L. garvieae. We found that measuring the serological response with a quantitative agglutinating antibody against the L. garvieae antigen (phenotype KG+) was an effective method of monitoring the potency of lactococcicosis vaccines. Vaccinated fish had significantly higher antibody titers than control fish when the L. garvieae Lg2-S strain was used as an antigen. Furthermore, the titer of the KG + agglutinating antibody was correlated with vaccine potency, and the cut-off titer was determined by comparing the data with those from the challenge test. An advantage of the proposed serology-based potency assay is that it will contribute to reduced numbers of animal deaths during vaccine potency evaluations.
authors.affiliation: National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries, 1-15-1 Tokura, Kokubunji, Tokyo 185-8511, Japan. Electronic address: aoki@nval.maff.go.jp.
authors.forename: Nao
authors.initials: N
authors.lastname: Nakajima
authors.affiliation: National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries, 1-15-1 Tokura, Kokubunji, Tokyo 185-8511, Japan.
authors.forename: Michiko
authors.initials: M
authors.lastname: Kawanishi
authors.affiliation: National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries, 1-15-1 Tokura, Kokubunji, Tokyo 185-8511, Japan.
authors.forename: Saiki
authors.initials: S
authors.lastname: Imamura
authors.affiliation: National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries, 1-15-1 Tokura, Kokubunji, Tokyo 185-8511, Japan.
authors.forename: Fumiya
authors.initials: F
authors.lastname: Hirano
authors.affiliation: National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries, 1-15-1 Tokura, Kokubunji, Tokyo 185-8511, Japan.
authors.forename: Mariko
authors.initials: M
authors.lastname: Uchiyama
authors.affiliation: National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries, 1-15-1 Tokura, Kokubunji, Tokyo 185-8511, Japan.
authors.forename: Kinya
authors.initials: K
authors.lastname: Yamamoto
authors.affiliation: National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries, 1-15-1 Tokura, Kokubunji, Tokyo 185-8511, Japan.
authors.forename: Hidetaka
authors.initials: H
authors.lastname: Nagai
authors.affiliation: Laboratory of Fish Health Management, Tokyo University of Marine Science and Technology, Tokyo 108-8477, Japan.
authors.forename: Kunihiko
authors.initials: K
authors.lastname: Futami
authors.affiliation: Laboratory of Fish Health Management, Tokyo University of Marine Science and Technology, Tokyo 108-8477, Japan.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Katagiri
authors.affiliation: Laboratory of Fish Health Management, Tokyo University of Marine Science and Technology, Tokyo 108-8477, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Maita
authors.affiliation: National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries, 1-15-1 Tokura, Kokubunji, Tokyo 185-8511, Japan.
authors.forename: Mayumi
authors.initials: M
authors.lastname: Kijima
mesh_terms: D000818:Animals; D001428:Bacterial Vaccines / Q000276:immunology*; D005393:Fish Diseases / Q000517:prevention & control*; D005399:Fishes; D016908:Gram-Positive Bacterial Infections / Q000517:prevention & control*; D017045:Lactococcus
chemical_list: D001428:Bacterial Vaccines
keywords: Lactococcus garvieae; Potency assay; Seriola; Vaccine