title: In vitro efficacy and release study with anti-inflammatory drugs incorporated in adhesive transdermal drug delivery systems.
abstract: The topical application of two different anti-inflammatory extracts incorporated in adhesive transdermal drug delivery systems (TDDSs) was investigated. Therefore, anti-inflammatory properties and percutaneous absorption behavior of adhesive TDDSs were characterized in vitro conducting experiments with a dermatologically relevant human skin model. Anti-inflammatory efficacy against UV irradiation of both TDDSs was determined in vitro with EpiDerm™. The reduction of the release of proinflammatory cytokines by topically applied TDDSs was compared with the reduction during the presence of the specific cyclooxygenase inhibitor diclofenac in the culture medium. A similar anti-inflammatory efficacy of the topically applied TDDSs in comparison with the use of diclofenac in the culture medium should be achieved. Furthermore, percutaneous absorption in efficacy tests was compared with percutaneous absorption in diffusion studies with porcine cadaver skin. Both the topically applied TDDSs showed a significant anti-inflammatory activity. Permeation coefficients through the stratum corneum and the epidermis gained from the release studies on porcine cadaver skin (Magnolia: 2.23·10(-5) cm/h, licorice: 4.68·10(-6) cm/h) were approximately five times lower than the permeation coefficients obtained with the EpiDerm™ skin model (Magnolia: 9.48·10(-5) cm/h, licorice: 24.0·10(-6) cm/h). Therefore, an adjustment of drug doses during experiments with the EpiDerm™ skin model because of weaker skin barrier properties should be considered.
authors.forename: Stefanie
authors.initials: S
authors.lastname: Meyer
authors.forename: Nils
authors.initials: N
authors.lastname: Peters
authors.forename: Tobias
authors.initials: T
authors.lastname: Mann
authors.forename: Rainer
authors.initials: R
authors.lastname: Wolber
authors.forename: Ralf
authors.initials: R
authors.lastname: Pörtner
authors.forename: Jens
authors.initials: J
authors.lastname: Nierle
mesh_terms: D000269:Adhesives / Q000737:chemistry*; D000279:Administration, Cutaneous; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage* / Q000493:pharmacokinetics / Q000494:pharmacology*; D016207:Cytokines / Q000276:immunology; D004008:Diclofenac / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000494:pharmacology; D016503:Drug Delivery Systems / Q000295:instrumentation; D006035:Glycyrrhiza / Q000737:chemistry; D006801:Humans; D031566:Magnolia / Q000737:chemistry; D010936:Plant Extracts / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000494:pharmacology; D012867:Skin / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism / Q000528:radiation effects; D012869:Skin Absorption; D013552:Swine; D057968:Transdermal Patch; D014466:Ultraviolet Rays
chemical_list: D000269:Adhesives; D000893:Anti-Inflammatory Agents; D016207:Cytokines; D010936:Plant Extracts; D004008:Diclofenac