title: Strategic siRNA screening approaches to target cancer at the Cancer Research UK Beatson Institute.
abstract: The RNAi Screening Facility at the Cancer Research UK Beatson Institute combines siRNA genome-wide screening with drug screening coupled with High Content Imaging and fluorescence-based phenotypic assays to target multiple types of cancer. Here, we describe the infrastructure of the Facility and the approaches we utilise. We also share our experiences in running such a facility and developing and executing screening campaigns, with particular regard to high content multiparametric analysis, data management and statistical analysis.
authors.affiliation: Cancer Research UK Beatson Institute, Garscube Estate, Switchback Road, Bearsden, Glasgow, G61 1BD, UK. e.shanks@beatson.gla.ac.uk.
authors.forename: Emma J
authors.initials: EJ
authors.lastname: Shanks
mesh_terms: D035843:Biomedical Research / Q000458:organization & administration*; D003627:Data Interpretation, Statistical; D016208:Databases, Factual; D004354:Drug Screening Assays, Antitumor / Q000379:methods*; D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D009369:Neoplasms / Q000235:genetics*; D010641:Phenotype; D034622:RNA Interference; D006113:United Kingdom