title: Detection of persistent organic compounds from biomethanated distillery spent wash (BMDS) and their degradation by manganese peroxidase and laccase producing bacterial strains.
abstract: Biomethanated distillery spent wash (BMDS) retains dark black colour with complex persistent organic pollutants even after anaerobic treatment. The specific ratio (4:3:1:1) of Proteus mirabilis (FJ581028), Bacillus sp. (FJ581030), Raoultella planticola (GU329705) and Enterobacter sakazakii (FJ581031) decolourised BMDS up to 76% within 192 hr along with degradation of persistent organic compounds in presence of glucose (1%) and peptone (0.1%). The colour removal ability was noted due to ligninolytic enzyme activity. Where the maximum manganese peroxidase was 1.93 U ml(-1) and laccase activity equalled 0.84 U ml(-1). The gas chromatography-mass spectrophotometry (GC-MS) analysis confirmed the direct correlation between colourant and persistent organic pollutants due to simultaneous reduction of colour and pollutants present in BMDS. The seed germination test showed reduction of 75% toxicity after bacterial treatment process.
authors.forename: Sangeeta
authors.initials: S
authors.lastname: Yadav
authors.forename: Ram
authors.initials: R
authors.lastname: Chandra
mesh_terms: D001419:Bacteria / Q000201:enzymology* / Q000378:metabolism; D001673:Biodegradation, Environmental; D008401:Gas Chromatography-Mass Spectrometry; D042845:Laccase / Q000378:metabolism*; D009930:Organic Chemicals / Q000378:metabolism*; D010544:Peroxidases / Q000378:metabolism*; D014865:Waste Disposal, Fluid
chemical_list: D009930:Organic Chemicals; D042845:Laccase; D010544:Peroxidases; C051129:manganese peroxidase