title: [The taxonomy of the Khasan virus (KHAV), a new representative of phlebovirus genera (Bunyaviridae), isolated from the ticks haemaphysalis longicornis (Neumann, 1901) in the Maritime Territory (Russia)].
abstract: The Khasan virus (KHAV) was originally isolated in Khasansky District and Maritime Territory in 1971 from the ticks Haemophysalis longicornis (Neumann, 1901) collected from the deers Cervus nippon (Temmink, 1838). Based on the biological properties and virion morphology, KHAV was identified as an unclassified member of the Bunyaviridae family. In order to elucidate the KHAV taxonomy in more detail, viral genome was partially sequenced using the next-generation sequencing technology. According to the phylogenetic analysis conducted for partial sequences of the three genome segments, KHAV was attributed to the genus Phlebovirus. KHAV is phylogenetically mostly related to the viruses of the Uukuniemi group and has up to 62% identity with them. The maximum identity level is observed for sequences of the RNA-dependent-RNA-polymerase (RdRp) gene. The KHAV homology level with the tick-borne Uukuniemi group viruses is 50 to 62%; however, for the mosquito-borne phleboviruses it does not exceed 30%.
authors.forename: S V
authors.initials: SV
authors.lastname: Al'khovskiĭ
authors.forename: D K
authors.initials: DK
authors.lastname: L'vov
authors.forename: M Iu
authors.initials: MIu
authors.lastname: Shchelkanov
authors.forename: A M
authors.initials: AM
authors.lastname: Shchetinin
authors.forename: P G
authors.initials: PG
authors.lastname: Deriabin
authors.forename: E I
authors.initials: EI
authors.lastname: Samokhvalov
authors.forename: A K
authors.initials: AK
authors.lastname: Gitel'man
authors.forename: A G
authors.initials: AG
authors.lastname: Botikov
mesh_terms: D000818:Animals; D002044:Bunyaviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D003670:Deer / Q000821:virology; D016679:Genome, Viral; D015698:Genomic Library; D016856:Phlebovirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012324:RNA-Dependent RNA Polymerase / Q000145:classification* / Q000235:genetics; D012426:Russia / Q000453:epidemiology; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013987:Ticks / Q000821:virology*; D014764:Viral Proteins / Q000145:classification* / Q000235:genetics
chemical_list: D014764:Viral Proteins; D012324:RNA-Dependent RNA Polymerase