title: Molecularly imprinted polymer microspheres for optical measurement of ultra trace nonfluorescent cyhalothrin in honey.
abstract: In this study, we first present a general protocol for making fluorescent molecularly imprinted polymer microspheres via precipitation polymerisation. We first prepared the fluorescent molecularly imprinted polymer microspheres upon copolymerisation of acrylamide with a small quantity of allyl fluorescein in the presence of cyhalothrin to form recognition sites without doping. The as-synthesised microspheres exhibited spherical shape, high fluorescence intensity and highly selective recognition. Under optical conditions, polymer microspheres were successfully applied to selectively and sensitively detect cyhalothrin, and a linear relationship could be obtained covering the lower concentration range of 0-1.0nM with a correlation coefficient of 0.9936 described by the Stern-Volmer equation. A lower limit of detection was found to be 0.004nM. The results of practical detection suggested that the developed method was satisfactory for determination of cyhalothrin in honey samples. This study therefore demonstrated the potential of molecularly imprinted polymers for detection of cyhalothrin in food.
authors.affiliation: School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, People's Republic of China.
authors.forename: Lin
authors.initials: L
authors.lastname: Gao
authors.affiliation: School of Material Science and Engineering, Jiangsu University, Zhenjiang 212013, People's Republic of China.
authors.forename: Xiuying
authors.initials: X
authors.lastname: Li
authors.affiliation: School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, People's Republic of China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: School of Material Science and Engineering, Jiangsu University, Zhenjiang 212013, People's Republic of China.
authors.forename: Jiangdong
authors.initials: J
authors.lastname: Dai
authors.affiliation: School of Material Science and Engineering, Jiangsu University, Zhenjiang 212013, People's Republic of China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Wei
authors.affiliation: School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, People's Republic of China.
authors.forename: Zhilong
authors.initials: Z
authors.lastname: Song
authors.affiliation: School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, People's Republic of China. Electronic address: yys@mail.ujs.edu.cn.
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Yan
authors.affiliation: School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, People's Republic of China.
authors.forename: Chunxiang
authors.initials: C
authors.lastname: Li
mesh_terms: D005453:Fluorescence; D005506:Food Contamination / Q000032:analysis*; D006722:Honey / Q000032:analysis*; D008863:Microspheres; D054802:Molecular Imprinting; D009570:Nitriles / Q000737:chemistry* / Q000302:isolation & purification*; D010573:Pesticide Residues / Q000737:chemistry / Q000302:isolation & purification*; D058105:Polymerization; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D011722:Pyrethrins / Q000737:chemistry* / Q000302:isolation & purification*
chemical_list: D009570:Nitriles; D010573:Pesticide Residues; D011108:Polymers; D011722:Pyrethrins; C037304:cyhalothrin
keywords: Fluoresce detection; Molecular imprinted polymer; Precipitation polymerisation; Pyrethroid pesticides; Ultra trace