title: High neuropilin 1 expression was associated with angiogenesis and poor overall survival in resected pancreatic ductal adenocarcinoma.
abstract: OBJECTIVE
  Neuropilin 1 (NRP-1) appears to promote angiogenesis by acting as a coreceptor with vascular endothelial growth factor receptor. We correlated NRP-1 expression with microvessel density (MVD) and overall survival (OS) in human pancreatic ductal adenocarcinomas (PDACs).
  
  
  METHODS
  Neuropilin 1 expression was graded semiquantitatively using immunohistochemistry in patients with resected PDAC. Moreover, MVD was determined with an anti-CD31 antibody staining. Expression of NRP-1 was correlated with MVD and clinicopathologic features in patients with PDAC. Overall survival effects of NRP-1 expression were evaluated by multivariate Cox regression and Kaplan-Meier analyses.
  
  
  RESULTS
  High NRP-1 expression was associated with advanced Union for International Cancer Control stage (P = 0.046), T stage (P = 0.031), and lymph node invasion (P = 0.045). Microvessel density was significantly higher in the tumors with high NRP-1 expression than that in the tumors with low NRP-1 expression (mean, 13.9 [SD, 9.1] vs 10.2 [SD, 7.2] per high-power field; P = 0.001). The multivariate Cox regression analysis demonstrated that high NRP-1 expression was independently associated with reduced OS (hazard ratio, 2.10; 95% confidence interval, 1.19-3.70).
  
  
  CONCLUSIONS
  Neuropilin 1 is highly expressed in PDACs, and high expression of NRP-1 is significantly correlated with angiogenesis, advanced tumor-node-metastasis stage, p T stage, node invasion, and poor postoperative OS.
authors.affiliation: From the *Department of Gastroenterology, Ruijin Hospital, Shanghai Jiaotong University; †Department of Pathology, Changhai Hospital of Second Military Medical University; ‡Department of General Surgery, Ruijin Hospital, Shanghai Jiaotong University; and §Department of Gastroenterology, Changhai Hospital of Second Military Medical University, Shanghai, China.
authors.forename: Qiwen
authors.initials: Q
authors.lastname: Ben
authors.forename: Jianming
authors.initials: J
authors.lastname: Zheng
authors.forename: Jian
authors.initials: J
authors.lastname: Fei
authors.forename: Wei
authors.initials: W
authors.lastname: An
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.forename: Zhaoshen
authors.initials: Z
authors.lastname: Li
authors.forename: Yaozong
authors.initials: Y
authors.lastname: Yuan
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D021441:Carcinoma, Pancreatic Ductal / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D009367:Neoplasm Staging; D009389:Neovascularization, Pathologic / Q000378:metabolism*; D039942:Neuropilin-1 / Q000096:biosynthesis*; D010190:Pancreatic Neoplasms / Q000378:metabolism* / Q000473:pathology; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D055815:Young Adult
chemical_list: D019408:Platelet Endothelial Cell Adhesion Molecule-1; D039942:Neuropilin-1