title: Cell-free protein synthesis: search for the happy middle.
abstract: Cell-free protein synthesis (CFPS) is a versatile technique gaining popularity because it allows researchers access to on-demand production of proteins. This Commentary by Zachary Russ and John Dueber discusses the latest research article by Rui Gan and Michael Jewett, which reports a convenient and cost-effective methodology for the preparation of Saccharomyces cerevisiae-based CFPS reactions.
authors.affiliation: Department of Bioengineering, University of California, Berkeley, CA, USA; Energy Biosciences Institute, University of California, Berkeley, CA, USA.
authors.forename: Zachary N
authors.initials: ZN
authors.lastname: Russ
authors.forename: John E
authors.initials: JE
authors.lastname: Dueber
mesh_terms: D001709:Biotechnology / Q000379:methods*; D002474:Cell-Free System; D011994:Recombinant Proteins / Q000235:genetics* / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics*
chemical_list: D011994:Recombinant Proteins