title: Reconstruction of small diameter arteries using decellularized vascular scaffolds.
abstract: Although artificial vessels are available for large diameter arteries, there are no artificial vessels for small diameter arteries of < 4 mm. We created a decellularized vascular scaffold (length, 10 mm; outer diameter, 1.5 mm; inner diameter, 1.3 mm) from rat abdominal arteries. We measured the biomechanical characteristics of the scaffolds, implanted them to defects made in rat carotid arteries, and evaluated their patency and the endothelial cell linings. Silastic grafts were implanted as controls. The decellularized scaffolds demonstrated similar mechanical characteristics to normal arteries. All of the control grafts were occluded. Fibroblast-like cells were discovered in the thrombus, and fibrous organization was apparent. In contrast, patency of the grafts in 10 of 12 animals was observed 4 weeks after implantation. The internal cavity of the patent scaffold was completely lined by endotheliallike cells. Thus, the possibility of small artery reconstruction using decellularized scaffolds was demonstrated.
authors.affiliation: Medical and Dental Device Technology Incubation Center, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Nagaoka
authors.affiliation: Graduate School of Life Science and System Engineering, Kyusyu Institute of Technology, Kita-Kyusyu,Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yamada
authors.affiliation: Medical and Dental Device Technology Incubation Center, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Kimura
authors.affiliation: Medical and Dental Device Technology Incubation Center, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University.
authors.forename: Akio
authors.initials: A
authors.lastname: Kishida
authors.affiliation: Medical Engineering Research Center, Osaka Institute of Technology, Osaka, Japan.
authors.forename: Toshia
authors.initials: T
authors.lastname: Fujisato
authors.affiliation: Medical and Dental Device Technology Incubation Center, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Takakuda
mesh_terms: D064591:Allografts / Q000637:transplantation; D000714:Anastomosis, Surgical / Q000379:methods; D000818:Animals; D001012:Aorta, Abdominal / Q000637:transplantation; D001158:Arteries / Q000473:pathology / Q000601:surgery*; D001672:Biocompatible Materials / Q000737:chemistry; D001696:Biomechanical Phenomena; D001807:Blood Vessel Prosthesis; D002339:Carotid Arteries / Q000601:surgery; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D004548:Elasticity; D042783:Endothelial Cells / Q000473:pathology; D004730:Endothelium, Vascular / Q000473:pathology; D006083:Graft Occlusion, Vascular / Q000209:etiology; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000473:pathology; D011474:Prosthesis Design; D051381:Rats; D017207:Rats, Sprague-Dawley; D019651:Plastic Surgery Procedures / Q000379:methods*; D013314:Stress, Mechanical; D013927:Thrombosis / Q000209:etiology; D023822:Tissue Engineering / Q000379:methods; D014021:Tissue Preservation / Q000379:methods; D054457:Tissue Scaffolds; D014654:Vascular Patency / Q000502:physiology
chemical_list: D001672:Biocompatible Materials; D004129:Dimethylpolysiloxanes; C013830:baysilon