title: Recognition of RNA duplex by a neomycin-Hoechst 33258 conjugate.
abstract: DNA minor groove binding drugs such as Hoechst 33258 have been shown to bind to a number of RNA structures. Similarly, RNA binding ligands such as neomycin have been shown by us to bind to a number of A-form DNA structures. A neomycin-Hoechst 33258 conjugate was recently shown to bind B-DNA, where Hoechst exhibits high affinity for the minor groove of A/T tract DNA and neomycin docks into the major groove. Further studies now indicate that the Hoechst moiety of the conjugate can be driven to bind RNA duplex as a consequence of neomycin binding in the RNA major groove. This is the first example of Hoechst 33258 binding to RNA duplex not containing bulges or loop motifs.
authors.affiliation: Laboratories of Medicinal Chemistry, Clemson University, Clemson, SC 29634, United States.
authors.forename: Bert
authors.initials: B
authors.lastname: Willis
authors.affiliation: Laboratories of Medicinal Chemistry, Clemson University, Clemson, SC 29634, United States. Electronic address: dparya@clemson.edu.
authors.forename: Dev P
authors.initials: DP
authors.lastname: Arya
mesh_terms: D006690:Bisbenzimidazole / Q000737:chemistry*; D005601:Framycetin / Q000737:chemistry*; D015394:Molecular Structure; D011108:Polymers / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*; D012330:RNA, Double-Stranded
chemical_list: D011108:Polymers; D012330:RNA, Double-Stranded; D005601:Framycetin; D012313:RNA; D006690:Bisbenzimidazole
keywords: DNA; Hoechst 33258; Neomycin; RNA