title: Intraspecific genetic variation and phylogenetic analysis of Dirofilaria immitis samples from Western China using complete ND1 and 16S rDNA gene sequences.
abstract: Dirofilaria immitis (heartworm) is the causative agent of an important zoonotic disease that is spread by mosquitoes. In this study, molecular and phylogenetic characterization of D. immitis were performed based on complete ND1 and 16S rDNA gene sequences, which provided the foundation for more advanced molecular diagnosis, prevention, and control of heartworm diseases. The mutation rate and evolutionary divergence in adult heartworm samples from seven dogs in western China were analyzed to obtain information on genetic diversity and variability. Phylogenetic relationships were inferred using both maximum parsimony (MP) and Bayes methods based on the complete gene sequences. The results suggest that D. immitis formed an independent monophyletic group in which the 16S rDNA gene has mutated more rapidly than has ND1.
authors.affiliation: Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an City, Sichuan Province, People's Republic of China; Department of Chemistry, College of Life and Science, Sichuan Agricultural University, Ya'an City, Sichuan Province, People's Republic of China.
authors.forename: Tianyu
authors.initials: T
authors.lastname: Liu
authors.affiliation: Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an City, Sichuan Province, People's Republic of China; Department of Chemistry, College of Life and Science, Sichuan Agricultural University, Ya'an City, Sichuan Province, People's Republic of China.
authors.forename: Yinan
authors.initials: Y
authors.lastname: Liang
authors.affiliation: Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an City, Sichuan Province, People's Republic of China; Department of Chemistry, College of Life and Science, Sichuan Agricultural University, Ya'an City, Sichuan Province, People's Republic of China.
authors.forename: Xiuqin
authors.initials: X
authors.lastname: Zhong
authors.affiliation: Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an City, Sichuan Province, People's Republic of China; Department of Chemistry, College of Life and Science, Sichuan Agricultural University, Ya'an City, Sichuan Province, People's Republic of China.
authors.forename: Ning
authors.initials: N
authors.lastname: Wang
authors.affiliation: Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an City, Sichuan Province, People's Republic of China; Department of Chemistry, College of Life and Science, Sichuan Agricultural University, Ya'an City, Sichuan Province, People's Republic of China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Hu
authors.affiliation: Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an City, Sichuan Province, People's Republic of China; Department of Chemistry, College of Life and Science, Sichuan Agricultural University, Ya'an City, Sichuan Province, People's Republic of China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an City, Sichuan Province, People's Republic of China; Department of Chemistry, College of Life and Science, Sichuan Agricultural University, Ya'an City, Sichuan Province, People's Republic of China.
authors.forename: Xiaobin
authors.initials: X
authors.lastname: Gu
authors.affiliation: Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an City, Sichuan Province, People's Republic of China; Department of Chemistry, College of Life and Science, Sichuan Agricultural University, Ya'an City, Sichuan Province, People's Republic of China.
authors.forename: Xuerong
authors.initials: X
authors.lastname: Peng
authors.affiliation: Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an City, Sichuan Province, People's Republic of China; Department of Chemistry, College of Life and Science, Sichuan Agricultural University, Ya'an City, Sichuan Province, People's Republic of China guangyouyang@hotmail.com.
authors.forename: Guangyou
authors.initials: G
authors.lastname: Yang
mesh_terms: D000818:Animals; D001483:Base Sequence; D002681:China / Q000453:epidemiology; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004183:Dirofilaria immitis / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D004184:Dirofilariasis / Q000175:diagnosis / Q000453:epidemiology / Q000469:parasitology*; D004283:Dog Diseases / Q000175:diagnosis / Q000453:epidemiology / Q000469:parasitology*; D004285:Dogs; D019143:Evolution, Molecular; D014644:Genetic Variation; D015801:Helminth Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA / Q000662:veterinary
chemical_list: D018509:DNA, Helminth; D004275:DNA, Ribosomal; D015801:Helminth Proteins; D012336:RNA, Ribosomal, 16S