title: Nanoencapsulation of an active peptidic fraction from sea bream scales collagen.
abstract: Sea bream scales were subjected to enzymatic hydrolysis with Esperase, and a peptide fraction with a molecular mass <3kDa (F3) was isolated by ultrafiltration. F3 was encapsulated in nanoliposomes made of partially purified phosphatidylcholine (PC). Concentrations of 3.1% and 1mg/ml for PC and F3, respectively, were established as the best entrapment protocol by response surface methodology. The liposomes entrapment efficiency and zeta potential were 74.6±0.9% and -40.8±0.67mV, respectively. The liposome size ranged from 66.2 to 214nm, with a mean diameter of 90.3nm and a polydispersity index of 0.25. The antioxidant activity and ACE inhibitory activity of the encapsulated peptide fraction (L-F3) remained constant after 8days at 4°C. Encapsulation preserved the biological activities of F3, and could therefore be an alternative to improve the stability of these compounds when applied to a food product.
authors.affiliation: Instituto Ciencia y Tecnología de Alimentos y Nutrición (ICTAN, CSIC),(1) C/José Antonio Nováis, 10, Ciudad Universitaria, 28040 Madrid, Spain.
authors.forename: Mauricio
authors.initials: M
authors.lastname: Mosquera
authors.affiliation: Instituto Ciencia y Tecnología de Alimentos y Nutrición (ICTAN, CSIC),(1) C/José Antonio Nováis, 10, Ciudad Universitaria, 28040 Madrid, Spain.
authors.forename: Begoña
authors.initials: B
authors.lastname: Giménez
authors.affiliation: Laboratório de Microbiologia e Bioquímica Aplicada, Instituto de Ciência e Tecnologia de Alimentos, Universidade Federal do Rio Grande do Sul, Av. Benito Gonçalves 9500, 94501-970 Porto Alegre, Brazil.
authors.forename: Indjara Mallmann
authors.initials: IM
authors.lastname: da Silva
authors.affiliation: Laboratório de Microbiologia e Bioquímica Aplicada, Instituto de Ciência e Tecnologia de Alimentos, Universidade Federal do Rio Grande do Sul, Av. Benito Gonçalves 9500, 94501-970 Porto Alegre, Brazil.
authors.forename: Juliana Ferreira
authors.initials: JF
authors.lastname: Boelter
authors.affiliation: Instituto Ciencia y Tecnología de Alimentos y Nutrición (ICTAN, CSIC),(1) C/José Antonio Nováis, 10, Ciudad Universitaria, 28040 Madrid, Spain.
authors.forename: Pilar
authors.initials: P
authors.lastname: Montero
authors.affiliation: Instituto Ciencia y Tecnología de Alimentos y Nutrición (ICTAN, CSIC),(1) C/José Antonio Nováis, 10, Ciudad Universitaria, 28040 Madrid, Spain. Electronic address: mc.gomez@csic.es.
authors.forename: M Carmen
authors.initials: MC
authors.lastname: Gómez-Guillén
authors.affiliation: Laboratório de Microbiologia e Bioquímica Aplicada, Instituto de Ciência e Tecnologia de Alimentos, Universidade Federal do Rio Grande do Sul, Av. Benito Gonçalves 9500, 94501-970 Porto Alegre, Brazil.
authors.forename: Adriano
authors.initials: A
authors.lastname: Brandelli
mesh_terms: D000818:Animals; D003094:Collagen / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry; D021541:Sea Bream
chemical_list: D008081:Liposomes; D010455:Peptides; D003094:Collagen
keywords: Biological activities; Collagen peptides; Encapsulation; Fish scales; Liposomes; Nanovesicles