title: Upconverting crystal/dextran-g-DOPE with high fluorescence stability for simultaneous photodynamic therapy and cell imaging.
abstract: To date, the application of photodynamic therapy in deep tissue has been severely restricted by the limited penetration depth of excitation light, such as UV light and visible light. In this work, a protocol of upconverting crystal/dextran-g-DOPE nanocomplex (UCN/dextran-g-DOPE) was developed. The nanocomplex was assembled from the hydrophobic upconverting nanoparticle (UCN) core and hydrophilic lipid shell. The photosensitizer zinc phthalocyanine (ZnPc) loaded UCN/dextran-g-DOPE offers possibilities to overcome the problem mentioned above. The UCN core works as a transducer to convert deeply penetrating near-infrared light to visible light to activate ZnPc for photodynamic therapy. The dextran-g-DOPE lipid shell is used for loading ZnPc and protecting the whole system from nonspecific absorbance or corrosion during the transportation. The experiment results show that the nanocomplex is an individual sphere with an average size of 30 nm. The ZnPc was activated to produce singlet oxygen successfully by the upconverting fluorescence emitted from UCN. The nanocomplex has high fluorescence stability in alkaline or neutral buffer solutions. Importantly, the ZnPc loaded UCN/dextran-g-DOPE nanocomplex showed a significant inhibitory effect on tumor cells after NIR exposure. Our data suggest that a ZnPc loaded UCN/dextran-g-DOPE nanocomplex may be a useful nanoplatform for future PDT treatment in deep-cancer therapy based on the upconverting mechanism.
authors.forename: HanJie
authors.initials: H
authors.lastname: Wang
authors.forename: Sheng
authors.initials: S
authors.lastname: Wang
authors.forename: Zhongyun
authors.initials: Z
authors.lastname: Liu
authors.forename: Chunhong
authors.initials: C
authors.lastname: Dong
authors.forename: Jiumin
authors.initials: J
authors.lastname: Yang
authors.forename: Xiaoqun
authors.initials: X
authors.lastname: Gong
authors.forename: Jin
authors.initials: J
authors.lastname: Chang
mesh_terms: D001940:Breast / Q000473:pathology; D001943:Breast Neoplasms / Q000175:diagnosis* / Q000628:therapy; D045744:Cell Line, Tumor; D003911:Dextrans / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry; D005260:Female; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D007211:Indoles / Q000008:administration & dosage / Q000737:chemistry / Q000627:therapeutic use*; D054833:Isoindoles; D009942:Organometallic Compounds / Q000008:administration & dosage / Q000737:chemistry / Q000627:therapeutic use*; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010778:Photochemotherapy / Q000379:methods; D017319:Photosensitizing Agents / Q000008:administration & dosage / Q000737:chemistry / Q000627:therapeutic use*; D017967:Zinc Compounds
chemical_list: C410262:1,2-dioleoyl-glycero-3-phosphatidyl ethanolamine; D003911:Dextrans; D004337:Drug Carriers; D005456:Fluorescent Dyes; D007211:Indoles; D054833:Isoindoles; D009942:Organometallic Compounds; D010714:Phosphatidylethanolamines; D017319:Photosensitizing Agents; D017967:Zinc Compounds; C052159:Zn(II)-phthalocyanine