title: Resonance Raman spectroscopy of the oxygenated intermediates of human CYP19A1 implicates a compound i intermediate in the final lyase step.
abstract: CYP19A1, or aromatase, a cytochrome P450 responsible for estrogen biosynthesis in humans, is an important therapeutic target for the treatment of breast cancer. There is still controversy surrounding the identity of reaction intermediate that catalyzes carbon-carbon scission in this key enzyme. Probing the oxy-complexes of CYP19A1 poised for hydroxylase and lyase chemistries using resonance Raman spectroscopy and drawing a comparison with CYP17A1, we have found no significant difference in the frequencies or isotopic shifts for these two steps in CYP19A1. Our experiments implicate the involvement of Compound I in the terminal lyase step of CYP19A1 catalysis.
authors.affiliation: Department of Chemistry, Marquette University , Milwaukee, Wisconsin 53233, United States.
authors.forename: Piotr J
authors.initials: PJ
authors.lastname: Mak
authors.forename: Abhinav
authors.initials: A
authors.lastname: Luthra
authors.forename: Stephen G
authors.initials: SG
authors.lastname: Sligar
authors.forename: James R
authors.initials: JR
authors.lastname: Kincaid
mesh_terms: D000735:Androstenedione / Q000737:chemistry / Q000378:metabolism*; D001141:Aromatase / Q000378:metabolism*; D006801:Humans; D008190:Lyases / Q000378:metabolism*; D010084:Oxidation-Reduction; D010100:Oxygen / Q000737:chemistry / Q000378:metabolism; D013059:Spectrum Analysis, Raman
chemical_list: D000735:Androstenedione; D001141:Aromatase; C561410:CYP19A1 protein, human; D008190:Lyases; D010100:Oxygen