title: Human papillomavirus detection and p16INK4a expression in cervical lesions: a comparative study.
abstract: p16(INK4a) expression in dysplastic cervical lesions is related to high-risk human papillomavirus (HR-HPV) infection. The immunohistochemical expression of this protein in these lesions allows an increase in diagnostic reproducibility in biopsies and the introduction of prognostic factors in low-grade lesions. Here, we studied the immunohistochemical expression of p16 in 86 dysplastic cervical lesions, 54 cervical intraepithelial neoplasms-grade 1 (CIN-I), 23 CIN-II, and 9 CIN-III. In addition, we performed HPV detection and genotyping. We detected HR-HPV in 19/54 CIN-I, 21/23 CIN-II and 9/9 CIN-III cases. p16(INK4a) immunoreactivity was observed in 7/19 CIN-I HR-HPV-positive, 17/21 CIN-II HR-HPV-positive and all CIN-III cases. Immunoreactivity for p16(INK4a) was found in 7/54 CIN-I and in 17/23 CIN-II cases. In the follow-up, we detected 3 p16-positive high-grade squamous epithelial lesions (CIN-II and CIN-III) in the CIN-I/p16-negative group and 5 p16-positive high-grade squamous epithelial lesions cases in the CIN-II/p16-negative group. We conclude that p16 negativity in CIN-I and CIN-II biopsies does not always imply regression of the lesion and that the diagnosis of CIN-II should not be based solely on p16 results.
authors.affiliation: Department of Obstetrics and Gynecology, Hospital Universitari del Mar, 08003 Barcelona, Catalonia, Spain.
authors.forename: Jordi
authors.initials: J
authors.lastname: Genovés
authors.affiliation: Department of Pathology, Hospital Universitari del Mar, 08003 Barcelona, Catalonia, Spain; School of Medicine, Hospital Universitari del Mar, Universitat Autònoma de Barcelona, 08003 Barcelona, Catalonia, Spain. Electronic address: 86780@parcdesalutmar.cat.
authors.forename: Frances
authors.initials: F
authors.lastname: Alameda
authors.affiliation: Department of Obstetrics and Gynecology, Hospital Universitari del Mar, 08003 Barcelona, Catalonia, Spain; School of Medicine, Hospital Universitari del Mar, Universitat Autònoma de Barcelona, 08003 Barcelona, Catalonia, Spain.
authors.forename: Gemma
authors.initials: G
authors.lastname: Mancebo
authors.affiliation: Department of Obstetrics and Gynecology, Hospital Universitari del Mar, 08003 Barcelona, Catalonia, Spain.
authors.forename: Josep Maria
authors.initials: JM
authors.lastname: Solé
authors.affiliation: Department of Pathology, Hospital Universitari del Mar, 08003 Barcelona, Catalonia, Spain.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Bellosillo
authors.affiliation: Department of Pathology, Hospital Universitari del Mar, 08003 Barcelona, Catalonia, Spain.
authors.forename: Belen
authors.initials: B
authors.lastname: Lloveras
authors.affiliation: Department of Obstetrics and Gynecology, Hospital Universitari del Mar, 08003 Barcelona, Catalonia, Spain.
authors.forename: Silvia
authors.initials: S
authors.lastname: Agramunt
authors.affiliation: Department of Pathology, Hospital Universitari del Mar, 08003 Barcelona, Catalonia, Spain.
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Baró
authors.affiliation: Department of Pathology, Hospital Universitari del Mar, 08003 Barcelona, Catalonia, Spain.
authors.forename: Mercè
authors.initials: M
authors.lastname: Muset
authors.affiliation: Department of Obstetrics and Gynecology, Hospital Universitari del Mar, 08003 Barcelona, Catalonia, Spain.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Casado
authors.affiliation: Department of Pathology, Hospital Germans Trias i Pujol, Barcelona, Catalonia, Spain.
authors.forename: Laia
authors.initials: L
authors.lastname: Serrano
authors.affiliation: Department of Obstetrics and Gynecology, Hospital Universitari del Mar, 08003 Barcelona, Catalonia, Spain.
authors.forename: Esther
authors.initials: E
authors.lastname: Miralpeix
authors.affiliation: Department of Obstetrics and Gynecology, Hospital Universitari del Mar, 08003 Barcelona, Catalonia, Spain; School of Medicine, Hospital Universitari del Mar, Universitat Autònoma de Barcelona, 08003 Barcelona, Catalonia, Spain.
authors.forename: Ramon
authors.initials: R
authors.lastname: Carreras
mesh_terms: D000328:Adult; D014408:Biomarkers, Tumor / Q000032:analysis; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000032:analysis*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000175:diagnosis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D002583:Uterine Cervical Neoplasms / Q000378:metabolism / Q000821:virology*; D055815:Young Adult; D002578:Uterine Cervical Dysplasia / Q000378:metabolism / Q000821:virology*
chemical_list: D014408:Biomarkers, Tumor; D019941:Cyclin-Dependent Kinase Inhibitor p16
keywords: Dysplasia; HPV; Uterine cervix; p16(INK4a)