title: Antiulcer Effect of Extract/Fractions ofEruca sativa: Attenuation of Urease Activity.
abstract: Eruca sativa(Rocket salad) is known for its antiulcer properties in the traditional system of treatment. The present study was, therefore, designed to scrutinize its effect on urease activity in vitro. The results demonstrated marked attenuation of urease by the crude extract of various test concentrations with IC50value of 7.77 mg/mL. On fractionation, marked change in inhibitory profile was observed. The ethyl acetate fraction was the most potent urease inhibitor with IC50value of 4.17 mg/mL followed by the aqueous fraction with an IC50value of 5.83 mg/mL. However, hexane did not show significant urease inhibition. In conclusion, the present study illustrated strong antagonism of urease activity and thus validated scientifically the traditional use of the plant in the treatment of ulcers.
authors.affiliation: 1 Department of Pharmacy, Adbul Wali Khan University, Mardan, Pakistan.
authors.forename: Haroon
authors.initials: H
authors.lastname: Khan
authors.affiliation: 2 Department of Chemistry, Kohat University of Science and Technology, Kohat, Pakistan.
authors.forename: Murad Ali
authors.initials: MA
authors.lastname: Khan
keywords: extract/fractions; urease inhibition