title: Influence of surface-imprinted nanoparticles on trypsin activity.
abstract: Here, the modulation of enzyme activity is presented by protein-imprinted nanoparticles produced using a solid-phase approach. Using trypsin as target, binding of the nanoparticles to the enzyme results in its inhibition or in stabilization, depending on the orientation of the immobilized enzyme used during imprinting.
authors.affiliation: Department of Chemistry, University Road, Leicester, LE1 7RH, UK.
authors.forename: António
authors.initials: A
authors.lastname: Guerreiro
authors.forename: Alessandro
authors.initials: A
authors.lastname: Poma
authors.forename: Kal
authors.initials: K
authors.lastname: Karim
authors.forename: Ewa
authors.initials: E
authors.lastname: Moczko
authors.forename: Jessica
authors.initials: J
authors.lastname: Takarada
authors.forename: Isabel Perez
authors.initials: IP
authors.lastname: de Vargas-Sansalvador
authors.forename: Nicholas
authors.initials: N
authors.lastname: Turner
authors.forename: Elena
authors.initials: E
authors.lastname: Piletska
authors.forename: Cristiana Schmidt
authors.initials: CS
authors.lastname: de Magalhães
authors.forename: Natalia
authors.initials: N
authors.lastname: Glazova
authors.forename: Anastasia
authors.initials: A
authors.lastname: Serkova
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Omelianova
authors.forename: Sergey
authors.initials: S
authors.lastname: Piletsky
mesh_terms: D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism*; D054802:Molecular Imprinting / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods; D020349:Surface Plasmon Resonance; D014357:Trypsin / Q000737:chemistry / Q000378:metabolism*
chemical_list: D001672:Biocompatible Materials; D004800:Enzymes, Immobilized; D014357:Trypsin
keywords: enzyme inhibition; molecular recognition; molecularly imprinted polymers; nanoparticles; supramolecular chemistry