title: Identification of myo-inositol hexakisphosphate (IP6) as a β-secretase 1 (BACE1) inhibitory molecule in rice grain extract and digest.
abstract: Alzheimer's disease (AD) is widely considered to be caused by amyloid-β peptide (Aβ) accumulation in the brain. Aβ is excised from amyloid-β precursor protein through sequential cleavage by β-secretase 1 (BACE1) and γ-secretase. Thus, BACE1 inhibition could prevent Aβ accumulation. Here, we identified myo-inositol hexakisphosphate (IP6) as a BACE1 inhibitory molecule in rice grain extract and digest. The rice digest and IP6 significantly inhibited Aβ production in neuroblastoma cells without cytotoxicity. These results suggested that rice components, including IP6, may be promising starting materials for the development of potent and safe drugs and/or food to prevent AD.
authors.affiliation: Department of Materials Science and Technology, Faculty of Engineering, Niigata University, Ikarashi 2-8050, Niigata 950-2181, Japan.
authors.forename: Takako K
authors.initials: TK
authors.lastname: Abe
authors.affiliation: Department of Materials Science and Technology, Faculty of Engineering, Niigata University, Ikarashi 2-8050, Niigata 950-2181, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Taniguchi
keywords: AD, Alzheimer’s disease; APP, amyloid-β precursor protein; Alzheimer’s disease; Aβ; Aβ, amyloid-β peptide; BACE1; BACE1, β-secretase 1/beta-site APP cleaving enzyme 1; IC50, half-maximal inhibitory concentration; IP6; IP6 (PS), phytic acid dipotassium salt; IP6 (SS), phytic acid sodium salt; IP6, myo-inositol hexakisphosphate/phytic acid; Rice grain; SH-SY5Y cells; β-CTF, C-terminal fragment of β-cleaved APP