title: A total solid-phase synthesis of DILP8.
abstract: We have developed a cysteine anchoring method for the synthesis of DILP8 and its analogues. The first is to synthesis of DILP8A SS13-18, C14-MeOBzl, C24-Acm and activate it as DILP8A S13-18, C14-SSPyr C24-Acm. A next step is to synthesize the DILP8BC16-Acm. The desired peptide, DILP8 with Cys(Acm) at A-24 and B-16, was then dissolved in 75% HOAc by addition of Iodine in MeOH and 4M HCl in dioxane. The reaction mixture was monitored by HPLC and the excess iodine was reduced with ascorbic acid. Purification of the peptide was achieved by HPLC. Pure synthetic DILP8 showed a single peak on analytical HPLC with corrected molecular ion. By using the above methods, enough peptide and highly homogenous pure DLP8 were generated.
authors.forename: Aydar
authors.initials: A
authors.lastname: Sabirov
authors.forename: Rong-Ming
authors.initials: RM
authors.lastname: Lyu
authors.forename: Xiang-Qun
authors.initials: XQ
authors.lastname: Chen
authors.forename: Qing
authors.initials: Q
authors.lastname: Tian
authors.forename: Jaw-Kang
authors.initials: JK
authors.lastname: Chang
mesh_terms: D000818:Animals; D003545:Cysteine / Q000737:chemistry; D029721:Drosophila Proteins / Q000138:chemical synthesis* / Q000235:genetics / Q000302:isolation & purification*; D004331:Drosophila melanogaster; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000138:chemical synthesis* / Q000235:genetics / Q000302:isolation & purification*; D017386:Sequence Homology, Amino Acid; D060327:Solid-Phase Synthesis Techniques / Q000379:methods*
chemical_list: D029721:Drosophila Proteins; C101126:INSL4 protein, human; D036341:Intercellular Signaling Peptides and Proteins; C572149:insulin-like peptide 8, Drosophila; D003545:Cysteine