title: Multiple approaches to characterize the microbial community in a thermophilic anaerobic digester running on swine manure: a case study.
abstract: In this study, the first survey of microbial community in thermophilic anaerobic digester using swine manure as sole feedstock was performed by multiple approaches including denaturing gradient gel electrophoresis (DGGE), clone library and pyrosequencing techniques. The integrated analysis of 21 DGGE bands, 126 clones and 8506 pyrosequencing read sequences revealed that Clostridia from the phylum Firmicutes account for the most dominant Bacteria. In addition, our analysis also identified additional taxa that were missed by the previous researches, including members of the bacterial phyla Synergistetes, Planctomycetes, Armatimonadetes, Chloroflexi and Nitrospira which might also play a role in thermophilic anaerobic digester. Most archaeal 16S rRNA sequences could be assigned to the order Methanobacteriales instead of Methanomicrobiales comparing to previous studies. In addition, this study reported that the member of Methanothermobacter genus was firstly found in thermophilic anaerobic digester.
authors.affiliation: Department of Life Sciences, National Central University, No. 300 Jhongda Rd., Jhongli City 32001, Taiwan.
authors.forename: Nguyen Ngoc
authors.initials: NN
authors.lastname: Tuan
authors.affiliation: Department of Life Sciences, National Central University, No. 300 Jhongda Rd., Jhongli City 32001, Taiwan.
authors.forename: Yi-Chia
authors.initials: YC
authors.lastname: Chang
authors.affiliation: Institute of Urban Environment, Chinese Academy of Science, Xiamen 361021, China.
authors.forename: Chang-Ping
authors.initials: CP
authors.lastname: Yu
authors.affiliation: Department of Life Sciences, National Central University, No. 300 Jhongda Rd., Jhongli City 32001, Taiwan; Center for Biotechnology and Biomedical Engineering, National Central University, No. 300 Jhongda Rd., Jhongli City 32001, Taiwan. Electronic address: slhuang@cc.ncu.edu.tw.
authors.forename: Shir-Ly
authors.initials: SL
authors.lastname: Huang
mesh_terms: D000693:Anaerobiosis; D000818:Animals; D001105:Archaea / Q000145:classification* / Q000378:metabolism*; D001419:Bacteria / Q000145:classification* / Q000378:metabolism*; D058448:Biota; D016000:Cluster Analysis; D019641:DNA, Archaeal / Q000737:chemistry / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D058645:Denaturing Gradient Gel Electrophoresis; D006358:Hot Temperature; D008372:Manure / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013552:Swine
chemical_list: D019641:DNA, Archaeal; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D008372:Manure; D012336:RNA, Ribosomal, 16S
keywords: 16S rRNA; Anaerobic digestion; Metagenome; Pyrosequencing