title: Inhibitory effects of α-cyperone on adherence and invasion of avian pathogenic Escherichia coli O78 to chicken type II pneumocytes.
abstract: Avian pathogenic Escherichia coli (APEC) are extra-intestinal pathogenic E. coli, and usually cause avian septicemia through breaching the blood-gas barrier. Type II pneumocytes play an important role of maintaining the function of the blood-gas barrier. However, the mechanism of APEC injuring type II pneumocytes remains unclear. α-cyperone can inhibit lung cell injury induced by Staphylococcus aureus. In order to explore whether α-cyperone regulates the adherence and invasion of APEC-O78 to chicken type II pneumocytes, we successfully cultured chicken type II pneumocytes. The results showed that α-cyperone significantly decreased the adherence of APEC-O78 to chicken type II pneumocytes. In addition, α-cyperone inhibited actin cytoskeleton polymerization induced by APEC-O78 through down regulating the expression of Nck-2, Cdc42 and Rac1. These results provide new evidence for the prevention of colibacillosis in chicken.
authors.affiliation: Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, No. 5333 Xi'an Road, Changchun 130062, Jilin, China.
authors.forename: Li-Yan
authors.initials: LY
authors.lastname: Zhang
authors.affiliation: Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, No. 5333 Xi'an Road, Changchun 130062, Jilin, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Lv
authors.affiliation: Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, No. 5333 Xi'an Road, Changchun 130062, Jilin, China.
authors.forename: Shuai-Cheng
authors.initials: SC
authors.lastname: Wu
authors.affiliation: Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, No. 5333 Xi'an Road, Changchun 130062, Jilin, China.
authors.forename: Xun
authors.initials: X
authors.lastname: Guo
authors.affiliation: Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, No. 5333 Xi'an Road, Changchun 130062, Jilin, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Xia
authors.affiliation: Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, No. 5333 Xi'an Road, Changchun 130062, Jilin, China.
authors.forename: Xi-Rou
authors.initials: XR
authors.lastname: Hu
authors.affiliation: Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, No. 5333 Xi'an Road, Changchun 130062, Jilin, China.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Song
authors.affiliation: Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, No. 5333 Xi'an Road, Changchun 130062, Jilin, China.
authors.forename: Cui
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, No. 5333 Xi'an Road, Changchun 130062, Jilin, China.
authors.forename: Qian-Qian
authors.initials: QQ
authors.lastname: Qin
authors.affiliation: Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, No. 5333 Xi'an Road, Changchun 130062, Jilin, China. Electronic address: fubendong96@163.com.
authors.forename: Ben-Dong
authors.initials: BD
authors.lastname: Fu
authors.affiliation: Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, No. 5333 Xi'an Road, Changchun 130062, Jilin, China.
authors.forename: Peng-Fei
authors.initials: PF
authors.lastname: Yi
authors.affiliation: Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, No. 5333 Xi'an Road, Changchun 130062, Jilin, China.
authors.forename: Hai-Qing
authors.initials: HQ
authors.lastname: Shen
authors.affiliation: Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, No. 5333 Xi'an Road, Changchun 130062, Jilin, China.
authors.forename: Xu-Bin
authors.initials: XB
authors.lastname: Wei
mesh_terms: D056809:Alveolar Epithelial Cells; D000818:Animals; D001422:Bacterial Adhesion / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002645:Chickens; D004926:Escherichia coli / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D004927:Escherichia coli Infections / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D006801:Humans; D008171:Lung Diseases / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D009281:Naphthalenes / Q000494:pharmacology*; D011201:Poultry Diseases / Q000276:immunology / Q000382:microbiology* / Q000517:prevention & control*; D012329:RNA, Bacterial / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D013047:Specific Pathogen-Free Organisms
chemical_list: D009281:Naphthalenes; D012329:RNA, Bacterial; C583938:alpha-cyperone
keywords: APEC-O78; Actin cytoskeleton polymerization; Adherence; Chicken type II pneumocytes; Invasion; α-cyperone