title: Snail family members unequally trigger EMT and thereby differ in their ability to promote the neoplastic transformation of mammary epithelial cells.
abstract: By fostering cell commitment to the epithelial-to-mesenchymal transition (EMT), SNAIL proteins endow cells with motility, thereby favoring the metastatic spread of tumor cells. Whether the phenotypic change additionally facilitates tumor initiation has never been addressed. Here we demonstrate that when a SNAIL protein is ectopically produced in non-transformed mammary epithelial cells, the cells are protected from anoikis and proliferate under low-adherence conditions: a hallmark of cancer cells. The three SNAIL proteins show unequal oncogenic potential, strictly correlating with their ability to promote EMT. SNAIL3 especially behaves as a poor EMT-inducer comforting the concept that the transcription factor functionally diverges from its two related proteins.
authors.affiliation: Inserm UMR-S1052, Centre de Recherche en Cancérologie de Lyon, Lyon, France; CNRS UMR5286, Centre de Recherche en Cancérologie de Lyon, Lyon, France; LabEx DEVweCAN, Lyon, France; UNIV UMR1052, Lyon, France; Université de Lyon, Lyon, France; Centre Léon Bérard, Lyon, France.
authors.forename: Baptiste
authors.initials: B
authors.lastname: Gras
authors.affiliation: Inserm UMR-S1052, Centre de Recherche en Cancérologie de Lyon, Lyon, France; CNRS UMR5286, Centre de Recherche en Cancérologie de Lyon, Lyon, France; LabEx DEVweCAN, Lyon, France; UNIV UMR1052, Lyon, France; Université de Lyon, Lyon, France; Centre Léon Bérard, Lyon, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Jacqueroud
authors.affiliation: Inserm UMR-S1052, Centre de Recherche en Cancérologie de Lyon, Lyon, France; CNRS UMR5286, Centre de Recherche en Cancérologie de Lyon, Lyon, France; LabEx DEVweCAN, Lyon, France; UNIV UMR1052, Lyon, France; Université de Lyon, Lyon, France; Centre Léon Bérard, Lyon, France; ProfileXpert, Bron, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Wierinckx
authors.affiliation: Inserm UMR-S1052, Centre de Recherche en Cancérologie de Lyon, Lyon, France; CNRS UMR5286, Centre de Recherche en Cancérologie de Lyon, Lyon, France; LabEx DEVweCAN, Lyon, France; UNIV UMR1052, Lyon, France; Université de Lyon, Lyon, France; Centre Léon Bérard, Lyon, France.
authors.forename: Christelle
authors.initials: C
authors.lastname: Lamblot
authors.affiliation: Inserm UMR-S1052, Centre de Recherche en Cancérologie de Lyon, Lyon, France; CNRS UMR5286, Centre de Recherche en Cancérologie de Lyon, Lyon, France; LabEx DEVweCAN, Lyon, France; UNIV UMR1052, Lyon, France; Université de Lyon, Lyon, France; Centre Léon Bérard, Lyon, France.
authors.forename: Frédérique
authors.initials: F
authors.lastname: Fauvet
authors.affiliation: Inserm UMR-S1052, Centre de Recherche en Cancérologie de Lyon, Lyon, France; CNRS UMR5286, Centre de Recherche en Cancérologie de Lyon, Lyon, France; LabEx DEVweCAN, Lyon, France; UNIV UMR1052, Lyon, France; Université de Lyon, Lyon, France; Centre Léon Bérard, Lyon, France; ProfileXpert, Bron, France.
authors.forename: Joël
authors.initials: J
authors.lastname: Lachuer
authors.affiliation: Inserm UMR-S1052, Centre de Recherche en Cancérologie de Lyon, Lyon, France; CNRS UMR5286, Centre de Recherche en Cancérologie de Lyon, Lyon, France; LabEx DEVweCAN, Lyon, France; UNIV UMR1052, Lyon, France; Université de Lyon, Lyon, France; Centre Léon Bérard, Lyon, France; Institut Universitaire de France, Paris, France.
authors.forename: Alain
authors.initials: A
authors.lastname: Puisieux
authors.affiliation: Inserm UMR-S1052, Centre de Recherche en Cancérologie de Lyon, Lyon, France; CNRS UMR5286, Centre de Recherche en Cancérologie de Lyon, Lyon, France; LabEx DEVweCAN, Lyon, France; UNIV UMR1052, Lyon, France; Université de Lyon, Lyon, France; Centre Léon Bérard, Lyon, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Ansieau
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics / Q000473:pathology; D002448:Cell Adhesion / Q000235:genetics; D002470:Cell Survival / Q000235:genetics; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000473:pathology*; D004847:Epithelial Cells / Q000378:metabolism* / Q000473:pathology*; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D042361:Mammary Glands, Human / Q000473:pathology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D000071250:Snail Family Transcription Factors; D019098:Telomerase / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors; D019098:Telomerase