title: Characterization of CTX-M-14- and CTX-M-15-Producing Escherichia coli and Klebsiella pneumoniae Isolates from Urine Specimens in a Tertiary-Care Hospital.
abstract: This study aimed to characterize CTX-M producers of urinary E. coli and K. pneumoniae isolates and to determine the prevalence of plasmid-mediated antimicrobial resistance genes among them. Minimum inhibitory concentrations (MICs) were determined, and PCR and sequencing were performed. Among the 42 (82.3%) E. coli and 24 (77.4%) K. pneumoniae isolates containing bla(CTX-M), bla(CTX-M-14) and bla(CTX-M-15) were detected in 23 and 19 E. coli isolates, respectively, and in 7 and 17 K. pneumoniae isolates, respectively. CTX-M producers of urinary E. coli and K. pneumoniae were resistant to multiple antibiotics and contained other antimicrobial resistance genes. CTX-M-15 producers contained more antimicrobial resistance genes than did CTX-M-14 producers.
authors.affiliation: Department of Laboratory Medicine, College of Medicine, Chungnam National University, Daejeon 301-721, Republic of Korea.
authors.forename: Semi
authors.initials: S
authors.lastname: Kim
authors.forename: Ji Youn
authors.initials: JY
authors.lastname: Sung
authors.forename: Hye Hyun
authors.initials: HH
authors.lastname: Cho
authors.forename: Kye Chul
authors.initials: KC
authors.lastname: Kwon
authors.forename: Sun Hoe
authors.initials: SH
authors.lastname: Koo
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D024901:Drug Resistance, Multiple, Bacterial; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000382:microbiology* / Q000652:urine; D006801:Humans; D007710:Klebsiella Infections / Q000382:microbiology* / Q000652:urine; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*; D008826:Microbial Sensitivity Tests; D062606:Tertiary Care Centers / Q000706:statistics & numerical data; D014556:Urine / Q000382:microbiology*; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; C456080:beta-lactamase CTX-M-14; C473109:beta-lactamase CTX-M-15; D001618:beta-Lactamases