title: Gap43 transcription modulation in the adult brain depends on sensory activity and synaptic cooperation.
abstract: Brain development and learning is accompanied by morphological and molecular changes in neurons. The growth associated protein 43 (Gap43), indicator of neurite elongation and synapse formation, is highly expressed during early stages of development. Upon maturation of the brain, Gap43 is down-regulated by most neurons with the exception of subdivisions such as the CA3 region of hippocampus, the lateral superior olive (LSO) and the central inferior colliculus (CIC). Little is known about the regulation of this mRNA in adult brains. We found that the expression of Gap43 mRNA in specific neurons can be modulated by changing sensory activity of the adult brain. Using the central auditory system of rats as a model, Gap43 protein and mRNA levels were determined in LSO and CIC of hearing-experienced rats unilaterally or bilaterally deafened or unilaterally stimulated by a cochlear implant (CI). Our data indicate that Gap43 is a marker useful beyond monitoring neuronal growth and synaptogenesis, reflecting also specific patterns of synaptic activities on specific neurons. Thus, unilateral loss of input to an adult auditory system directly causes asymmetrical expression of Gap43 mRNA between LSOs or CICs on both sides of the brainstem. This consequence can be prevented by simple-patterned stimulation of a dysfunctional ear by way of a CI. We suggest that as a function of input balance and activity pattern, Gap43 mRNA expression changes as cells associate converging afferent signals.
authors.affiliation: Neurobiological Research Laboratory, Department of Otorhinolaryngology, University of Freiburg, Freiburg, Germany.
authors.forename: Nicole
authors.initials: N
authors.lastname: Rosskothen-Kuhl
authors.affiliation: Neurobiological Research Laboratory, Department of Otorhinolaryngology, University of Freiburg, Freiburg, Germany.
authors.forename: Robert-Benjamin
authors.initials: RB
authors.lastname: Illing
mesh_terms: D000818:Animals; D001306:Auditory Pathways; D001921:Brain / Q000378:metabolism*; D001933:Brain Stem / Q000378:metabolism; D005260:Female; D019922:GAP-43 Protein / Q000378:metabolism*; D007245:Inferior Colliculi / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
chemical_list: D019922:GAP-43 Protein