title: Effect of LLLT on autogenous bone grafts in the repair of critical size defects in the calvaria of immunosuppressed rats.
abstract: The aim of this study was to evaluate the effects of low-level laser therapy (LLLT) on the bone repair of critical size defects (CSDs) filled with autogenous bone in the calvaria of immunosuppressed rats. A 5 mm-diameter CSD was created in the calvaria of 30 rats. The animals were divided into 5 groups (n = 6): Control (C)--the defect was filled with a blood clot; Dexamethasone (D)--dexamethasone treatment, and the defect was filled with a blood clot; Autogenous bone (AB)--dexamethasone treatment, and the defect was filled with autogenous bone; LLLT--dexamethasone treatment, and the defect received LLLT (660 nm; 35 mW; 24.7 J/cm(2)); and AB + LLLT--dexamethasone treatment, and the defect was filled with autogenous bone and received LLLT. All animals were euthanized at 30 postoperative days. Histometric and histological analyses were performed. The new bone area (NBA) was calculated as the percentage of the total area of the original defect. Data were analysed statistically (an analysis of variance and Tukey's test; P < 0.05). The AB + LLLT group showed the largest NBA of all groups (P < 0.05). The use of LLLT with AB effectively stimulated bone formation in CSDs in the calvaria of immunosuppressed rats.
authors.affiliation: Group of Research and Study on Laser in Dentistry (GEPLO), Department of Surgery and Integrated Clinic, Division of Periodontics, São Paulo State University, UNESP ("Univ. Estadual Paulista"), Araçatuba, SP, Brazil; Department of Periodontics, University Center of the Educational Foundation of Barretos (UNIFEB), Barretos, SP, Brazil.
authors.forename: Valdir Gouveia
authors.initials: VG
authors.lastname: Garcia
authors.affiliation: Group of Research and Study on Laser in Dentistry (GEPLO), Department of Surgery and Integrated Clinic, Division of Periodontics, São Paulo State University, UNESP ("Univ. Estadual Paulista"), Araçatuba, SP, Brazil.
authors.forename: Angelita Strazzi
authors.initials: AS
authors.lastname: Sahyon
authors.affiliation: Group of Research and Study on Laser in Dentistry (GEPLO), Department of Surgery and Integrated Clinic, Division of Periodontics, São Paulo State University, UNESP ("Univ. Estadual Paulista"), Araçatuba, SP, Brazil.
authors.forename: Mariéllen
authors.initials: M
authors.lastname: Longo
authors.affiliation: Department of Clinic and Surgery, Federal of University Alfenas, Alfenas, MG, Brazil.
authors.forename: Leandro Araújo
authors.initials: LA
authors.lastname: Fernandes
authors.affiliation: Group of Research and Study on Laser in Dentistry (GEPLO), Department of Surgery and Integrated Clinic, Division of Periodontics, São Paulo State University, UNESP ("Univ. Estadual Paulista"), Araçatuba, SP, Brazil.
authors.forename: Erivan Clementino
authors.initials: EC
authors.lastname: Gualberto Junior
authors.affiliation: Group of Research and Study on Laser in Dentistry (GEPLO), Department of Surgery and Integrated Clinic, Division of Periodontics, São Paulo State University, UNESP ("Univ. Estadual Paulista"), Araçatuba, SP, Brazil.
authors.forename: Vivian Cristina Noronha
authors.initials: VC
authors.lastname: Novaes
authors.affiliation: Department of Basic Science, São Paulo State University, UNESP ("Univ. Estadual Paulista"), Araçatuba, SP, Brazil.
authors.forename: Edilson
authors.initials: E
authors.lastname: Ervolino
authors.affiliation: Group of Research and Study on Laser in Dentistry (GEPLO), Department of Surgery and Integrated Clinic, Division of Periodontics, São Paulo State University, UNESP ("Univ. Estadual Paulista"), Araçatuba, SP, Brazil.
authors.forename: Juliano Milanezi
authors.initials: JM
authors.lastname: de Almeida
authors.affiliation: Group of Research and Study on Laser in Dentistry (GEPLO), Department of Surgery and Integrated Clinic, Division of Periodontics, São Paulo State University, UNESP ("Univ. Estadual Paulista"), Araçatuba, SP, Brazil. Electronic address: letheodoro@uol.com.br.
authors.forename: Letícia Helena
authors.initials: LH
authors.lastname: Theodoro
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use; D064592:Autografts / Q000187:drug effects / Q000528:radiation effects* / Q000637:transplantation; D001777:Blood Coagulation / Q000502:physiology; D001847:Bone Diseases / Q000473:pathology / Q000532:radiotherapy / Q000601:surgery*; D001862:Bone Resorption / Q000473:pathology; D016025:Bone Transplantation / Q000379:methods*; D003094:Collagen / Q000187:drug effects / Q000528:radiation effects; D003238:Connective Tissue / Q000187:drug effects / Q000528:radiation effects; D003907:Dexamethasone / Q000627:therapeutic use; D005938:Glucocorticoids / Q000627:therapeutic use; D016867:Immunocompromised Host / Q000276:immunology; D007165:Immunosuppression Therapy / Q000379:methods*; D028022:Low-Level Light Therapy / Q000379:methods*; D008297:Male; D010012:Osteogenesis / Q000187:drug effects / Q000528:radiation effects; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012886:Skull / Q000187:drug effects / Q000528:radiation effects / Q000601:surgery*
chemical_list: D000893:Anti-Inflammatory Agents; D005938:Glucocorticoids; D003907:Dexamethasone; D003094:Collagen
keywords: Bone transplantation; Glucocorticoids; Immunocompromised host; Laser therapy