title: Expression ofmiR-486is a potential prognostic factor after nephrectomy in advanced renal cell carcinoma.
abstract: Renal cell carcinoma is the most frequently occurring neoplasm in the adult kidney, leading to poor prognosis. Therefore novel biomarkers are required for the prediction of early metastasis following nephrectomy. The aim of the present study was to investigate whether or not expression levels ofmiR-486, detected in RNA isolated from formalin-fixed paraffin-embedded tissue sections, can predict prognosis for patients with renal cell carcinoma (RCC). Expression levels ofmiR-486were measured by quantitative reverse transcriptase-polymerase chain reaction in 150 RCC cases. Expression ofmiR-486in RCC samples was ∼2.7-fold higher than in corresponding non-neoplastic kidney samples (P<0.0001). In stage III and IV RCC cases (n=46), a highmiR-486expression in tumors was associated with worse cancer-specific mortality, independent of clinical covariates, including TNM staging (P=0.0064). In addition,miR-486expression tended to be associated with cancer-specific mortality in stage III and IV RCC patients who were not treated with interferon-α (Kaplan-Meier analysis, n=14, P=0.0574). These results suggest thatmiR-486is a promising biomarker to identify poor prognosis in RCC patients. As expression ofmiR-486was measured from formalin-fixed paraffin-embedded (FFPE) samples, this study demonstrated that measurement ofmiR-486may be readily translated into clinical applications.
authors.affiliation: Departments of Molecular Pathology, Hiroshima University Institute of Biomedical and Health Sciences, Hiroshima 734-8551, Japan ; Urology, Hiroshima University Institute of Biomedical and Health Sciences, Hiroshima 734-8551, Japan.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Goto
authors.affiliation: Departments of Molecular Pathology, Hiroshima University Institute of Biomedical and Health Sciences, Hiroshima 734-8551, Japan.
authors.forename: Naohide
authors.initials: N
authors.lastname: Oue
authors.affiliation: Departments of Molecular Pathology, Hiroshima University Institute of Biomedical and Health Sciences, Hiroshima 734-8551, Japan ; Urology, Hiroshima University Institute of Biomedical and Health Sciences, Hiroshima 734-8551, Japan.
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Shinmei
authors.affiliation: Departments of Molecular Pathology, Hiroshima University Institute of Biomedical and Health Sciences, Hiroshima 734-8551, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Sentani
authors.affiliation: Departments of Molecular Pathology, Hiroshima University Institute of Biomedical and Health Sciences, Hiroshima 734-8551, Japan.
authors.forename: Naoya
authors.initials: N
authors.lastname: Sakamoto
authors.affiliation: Departments of Molecular Pathology, Hiroshima University Institute of Biomedical and Health Sciences, Hiroshima 734-8551, Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Naito
authors.affiliation: Urology, Hiroshima University Institute of Biomedical and Health Sciences, Hiroshima 734-8551, Japan.
authors.forename: Tetsutaro
authors.initials: T
authors.lastname: Hayashi
authors.affiliation: Urology, Hiroshima University Institute of Biomedical and Health Sciences, Hiroshima 734-8551, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Teishima
authors.affiliation: Urology, Hiroshima University Institute of Biomedical and Health Sciences, Hiroshima 734-8551, Japan.
authors.forename: Akio
authors.initials: A
authors.lastname: Matsubara
authors.affiliation: Departments of Molecular Pathology, Hiroshima University Institute of Biomedical and Health Sciences, Hiroshima 734-8551, Japan.
authors.forename: Wataru
authors.initials: W
authors.lastname: Yasui
keywords: miR-486; miRNA; prognosis; renal cell carcinoma