title: Reduction in DNA methyltransferases and alteration of DNA methylation pattern associate with mouse skin ageing.
abstract: Understanding molecular mechanisms of skin ageing is critical for developing effective anti-ageing strategies. Recently, it has been suggested that epigenetics maybe be involved in tissue ageing and age-related diseases; however, the evidence regarding skin ageing has been very limited. We ran a pilot study in mouse skin to test whether DNA methyltransferases (Dnmts), DNA demethylases such as ten-eleven translocation enzymes (Tets) and DNA methylation of gene promoters change with age by quantitative RT-PCR and methylated DNA immunoprecipitation (MeDIP)-chip. We discovered that the expression of Dnmt3a, Dnmt3b and Tet2 declines significantly with skin ageing. The genome-wide DNA methylation analysis indicates that both hypermethylation and hypomethylation in promoters of genes are taken place. Functional category of those genes suggests that inhibition of cell proliferation and activation of immune response are important adaptations likely induced by skin ageing. These findings shed new light on epigenetic regulation of skin ageing.
authors.affiliation: Department of Anesthesiology, Yale University School of Medicine, New Haven, CT, USA.
authors.forename: Hong
authors.initials: H
authors.lastname: Qian
authors.forename: Xiangru
authors.initials: X
authors.lastname: Xu
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D002875:Chromosomes / Q000648:ultrastructure; D004247:DNA / Q000032:analysis; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000235:genetics / Q000378:metabolism*; D019175:DNA Methylation; D000090205:DNA Methyltransferase 3A; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D049308:Dioxygenases; D044127:Epigenesis, Genetic; D005260:Female; D005786:Gene Expression Regulation; D007633:Keratins / Q000378:metabolism; D051379:Mice; D008811:Mice, Inbred DBA; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism*; D012867:Skin / Q000378:metabolism; D015595:Skin Aging; D000097584:DNA Methyltransferase 3B
chemical_list: D004247:DNA; D004248:DNA (Cytosine-5-)-Methyltransferases; D000090205:DNA Methyltransferase 3A; D004268:DNA-Binding Proteins; D049308:Dioxygenases; D007633:Keratins; D011518:Proto-Oncogene Proteins; D000097584:DNA Methyltransferase 3B; C000715535:Dnmt3a protein, mouse; C551914:Tet2 protein, mouse
keywords: DNA methylation; DNA methyltransferase; chromosome loci; gene expression; keratin; skin ageing; ten-eleven translocation enzyme