title: Long-term stability in biomass and production of terpene   indole alkaloids by hairy root culture of Rauvolfia serpentina   and cost approximation to endorse commercial realism.
abstract: The effect of 6 years of cultivation and use of table-sugar (TS) on the biomass/terpene alkaloid productivities and rol gene expression were studied in a hairy root (HR) clone of Rauvolfia serpentina. The media cost could be reduced >94 % by replacing sucrose (SUC) with TS—an unexplored avenue for HR cultivation. The overall productivities increased over long-term cultivation with sugar proving superior to SUC for biomass (24.4 ± 2.11 g/l DW after 40 days to 17.31 % higher) and reserpine (0.094 ± 0.008 % DW after 60 days to 193.8 % more) production. The latter however revealed comparatively better yields concerning ajmaline (0.507 ± 0.048 % DW after 60 days to 61.98 % higher) and yohimbine (0.628 ± 0.062 % DW after 60 days to 38.32 % higher), respectively. PCR amplification of rol genes confirmed long-term expression stability.
authors.forename: Pallavi
authors.initials: P
authors.lastname: Pandey
authors.forename: Ranjeet
authors.initials: R
authors.lastname: Kaur
authors.forename: Sailendra
authors.initials: S
authors.lastname: Singh
authors.forename: Sunil Kumar
authors.initials: SK
authors.lastname: Chattopadhyay
authors.forename: Santosh Kumar
authors.initials: SK
authors.lastname: Srivastava
authors.forename: Suchitra
authors.initials: S
authors.lastname: Banerjee
mesh_terms: D018533:Biomass; D001709:Biotechnology / Q000191:economics* / Q000379:methods; D003365:Costs and Cost Analysis; D026121:Indole Alkaloids / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism*; D011926:Rauwolfia / Q000378:metabolism*; D046948:Secologanin Tryptamine Alkaloids / Q000378:metabolism*; D013729:Terpenes / Q000378:metabolism*
chemical_list: D026121:Indole Alkaloids; D046948:Secologanin Tryptamine Alkaloids; D013729:Terpenes