title: Effect of ampicillin, streptomycin, penicillin and tetracycline on metal resistant and non-resistant Staphylococcus aureus.
abstract: There is an arising and concerning issue in the field of bacterial resistance, which is confirmed by the number of deaths associated with drug-resistant bacterial infections. The aim of this study was to compare the effects of antibiotics on Staphylococcus aureus non-resistant strain and strains resistant to cadmium or lead ions. Metal resistant strains were created by the gradual addition of 2 mM solution of metal ions (cadmium or lead) to the S. aureus culture. An increasing antimicrobial effect of ampicillin, streptomycin, penicillin and tetracycline (0, 10, 25, 50, 75, 150, 225 and 300 µM) on the resistant strains was observed using a method of growth curves. A significant growth inhibition (compared to control) of cadmium resistant cells was observed in the presence of all the four different antibiotics. On the other hand, the addition of streptomycin and ampicillin did not inhibit the growth of lead resistant strain. Other antibiotics were still toxic to the bacterial cells. Significant differences in the morphology of cell walls were indicated by changes in the cell shape. Our data show that the presence of metal ions in the urban environment may contribute to the development of bacterial strain resistance to other substances including antibiotics, which would have an impact on public health.
authors.affiliation: Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, Brno CZ-613 00, Czech Republic. dagmar.chudobova@centrum.cz.
authors.forename: Dagmar
authors.initials: D
authors.lastname: Chudobova
authors.affiliation: Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, Brno CZ-613 00, Czech Republic. esedinka@seznam.cz.
authors.forename: Simona
authors.initials: S
authors.lastname: Dostalova
authors.affiliation: Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, Brno CZ-613 00, Czech Republic. iva.blazkova@seznam.cz.
authors.forename: Iva
authors.initials: I
authors.lastname: Blazkova
authors.affiliation: Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, Brno CZ-613 00, Czech Republic. petrmichalek85@gmail.com.
authors.forename: Petr
authors.initials: P
authors.lastname: Michalek
authors.affiliation: Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, Brno CZ-613 00, Czech Republic. brano.ruttkay@seznam.cz.
authors.forename: Branislav
authors.initials: B
authors.lastname: Ruttkay-Nedecky
authors.affiliation: Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, Brno CZ-613 00, Czech Republic. msmatejsklenar@gmail.com.
authors.forename: Matej
authors.initials: M
authors.lastname: Sklenar
authors.affiliation: Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, Brno CZ-613 00, Czech Republic. lukasnejdl@gmail.com.
authors.forename: Lukas
authors.initials: L
authors.lastname: Nejdl
authors.affiliation: Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, Brno CZ-613 00, Czech Republic. george.kudr@centrum.cz.
authors.forename: Jiri
authors.initials: J
authors.lastname: Kudr
authors.affiliation: Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, Brno CZ-616 00, Czech Republic. j.gumulec@gmail.com.
authors.forename: Jaromir
authors.initials: J
authors.lastname: Gumulec
authors.affiliation: Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, Brno CZ-613 00, Czech Republic. severova@centrum.cz.
authors.forename: Katerina
authors.initials: K
authors.lastname: Tmejova
authors.affiliation: Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, Brno CZ-613 00, Czech Republic. mariekon@centrum.cz.
authors.forename: Marie
authors.initials: M
authors.lastname: Konecna
authors.affiliation: Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, Brno CZ-613 00, Czech Republic. Marketa.Ryvolova@seznam.cz.
authors.forename: Marketa
authors.initials: M
authors.lastname: Vaculovicova
authors.affiliation: Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, Brno CZ-616 00, Czech Republic. d.hynek@email.cz.
authors.forename: David
authors.initials: D
authors.lastname: Hynek
authors.affiliation: Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, Brno CZ-616 00, Czech Republic. masarik@med.muni.cz.
authors.forename: Michal
authors.initials: M
authors.lastname: Masarik
authors.affiliation: Karel Englis College, Sujanovo nam. 356/1, Brno CZ-602 00, Czech Republic. jindrak@email.cz.
authors.forename: Jindrich
authors.initials: J
authors.lastname: Kynicky
authors.affiliation: Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, Brno CZ-613 00, Czech Republic. kizek@sci.muni.cz.
authors.forename: Rene
authors.initials: R
authors.lastname: Kizek
authors.affiliation: Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, Brno CZ-613 00, Czech Republic. vojtech.adam@mendelu.cz.
authors.forename: Vojtech
authors.initials: V
authors.lastname: Adam
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002104:Cadmium / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D007854:Lead / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D013211:Staphylococcus aureus / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D002104:Cadmium; D007854:Lead