title: Application of TALEs, CRISPR/Cas and sRNAs as trans-acting regulators in prokaryotes.
abstract: The last several years have witnessed an explosion in the understanding and use of novel, versatile trans-acting elements. TALEs, CRISPR/Cas, and sRNAs can be easily fashioned to bind any specific sequence of DNA (TALEs, CRISPR/Cas) or RNA (sRNAs) because of the simple rules governing their interactions with nucleic acids. This unique property enables these tools to repress the expression of genes at the transcriptional or post-transcriptional levels, respectively, without prior manipulation of cis-acting and/or chromosomal target DNA sequences. These tools are now being harnessed by synthetic biologists, particularly those in the eukaryotic community, for genome-wide regulation, editing, or epigenetic studies. Here we discuss the exciting opportunities for using TALEs, CRISPR/Cas, and sRNAs as synthetic trans-acting regulators in prokaryotes.
authors.affiliation: University of Wisconsin-Madison, Department of Chemical and Biological Engineering, 3629 Engineering Hall, 1415 Engineering Drive, Madison, WI 53706, USA.
authors.forename: Matthew F
authors.initials: MF
authors.lastname: Copeland
authors.affiliation: University of Wisconsin-Madison, Department of Chemical and Biological Engineering, 3629 Engineering Hall, 1415 Engineering Drive, Madison, WI 53706, USA.
authors.forename: Mark C
authors.initials: MC
authors.lastname: Politz
authors.affiliation: University of Wisconsin-Madison, Department of Chemical and Biological Engineering, 3629 Engineering Hall, 1415 Engineering Drive, Madison, WI 53706, USA. Electronic address: pfleger@wisc.edu.
authors.forename: Brian F
authors.initials: BF
authors.lastname: Pfleger
mesh_terms: D000818:Animals; D064112:Clustered Regularly Interspaced Short Palindromic Repeats; D004247:DNA / Q000235:genetics; D005786:Gene Expression Regulation; D006801:Humans; D011387:Prokaryotic Cells; D012313:RNA / Q000235:genetics*; D015533:Transcriptional Activation
chemical_list: D012313:RNA; D004247:DNA