title: A single intake of capsiate improves mechanical performance and bioenergetics efficiency in contracting mouse skeletal muscle.
abstract: Capsiate is known to increase whole body oxygen consumption possibly via the activation of uncoupling processes, but its effect at the skeletal muscle level remains poorly documented and conflicting. To clarify this issue, gastrocnemius muscle function and energetics were investigated in mice 2 h after a single intake of either vehicle (control) or purified capsiate (at 10 or 100 mg/kg body wt) through a multidisciplinary approach combining in vivo and in vitro measurements. Mechanical performance and energy pathway fluxes were assessed strictly noninvasively during a standardized electrostimulation-induced exercise, using an original device implementing 31-phosphorus magnetic resonance spectroscopy, and mitochondrial respiration was evaluated in isolated saponin-permeabilized fibers. Compared with control, both capsiate doses produced quantitatively similar effects at the energy metabolism level, including an about twofold decrease of the mitochondrial respiration sensitivity for ADP. Interestingly, they did not alter either oxidative phosphorylation or uncoupling protein 3 gene expression at rest. During 6 min of maximal repeated isometric contractions, both doses reduced the amount of ATP produced from glycolysis and oxidative phosphorylation but increased the relative contribution of oxidative phosphorylation to total energy turnover (+28 and +21% in the 10- and 100-mg groups, respectively). ATP cost of twitch force generation was further reduced in the 10- (-35%) and 100-mg (-45%) groups. Besides, the highest capsiate dose also increased the twitch force-generating capacity. These data present capsiate as a helpful candidate to enhance both muscle performance and oxidative phosphorylation during exercise, which could constitute a nutritional approach for improving health and preventing obesity and associated metabolic disorders.
authors.affiliation: Aix-Marseille Université, Centre National de la Recherche Scientifique, Centre de Resonance Magnetique Biologique et Medicale UMR 7339, 13385, Marseille, France.
authors.forename: Yashiro
authors.initials: Y
authors.lastname: Kazuya
authors.affiliation: Aix-Marseille Université, Centre National de la Recherche Scientifique, Centre de Resonance Magnetique Biologique et Medicale UMR 7339, 13385, Marseille, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Tonson
authors.affiliation: Aix-Marseille Université, Centre National de la Recherche Scientifique, Centre de Resonance Magnetique Biologique et Medicale UMR 7339, 13385, Marseille, France.
authors.forename: Emilie
authors.initials: E
authors.lastname: Pecchi
authors.affiliation: Aix-Marseille Université, Centre National de la Recherche Scientifique, Centre de Resonance Magnetique Biologique et Medicale UMR 7339, 13385, Marseille, France.
authors.forename: Christiane
authors.initials: C
authors.lastname: Dalmasso
authors.affiliation: Aix-Marseille Université, Centre National de la Recherche Scientifique, Centre de Resonance Magnetique Biologique et Medicale UMR 7339, 13385, Marseille, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Vilmen
authors.affiliation: Aix-Marseille Université, Centre National de la Recherche Scientifique, Centre de Resonance Magnetique Biologique et Medicale UMR 7339, 13385, Marseille, France.
authors.forename: Yann Le
authors.initials: YL
authors.lastname: Fur
authors.affiliation: Aix-Marseille Université, Centre National de la Recherche Scientifique, Centre de Resonance Magnetique Biologique et Medicale UMR 7339, 13385, Marseille, France.
authors.forename: Monique
authors.initials: M
authors.lastname: Bernard
authors.affiliation: Aix-Marseille Université, Centre National de la Recherche Scientifique, Centre de Resonance Magnetique Biologique et Medicale UMR 7339, 13385, Marseille, France.
authors.forename: David
authors.initials: D
authors.lastname: Bendahan
authors.affiliation: Aix-Marseille Université, Centre National de la Recherche Scientifique, Centre de Resonance Magnetique Biologique et Medicale UMR 7339, 13385, Marseille, France benoit.giannesini@univ-amu.fr.
authors.forename: Benoît
authors.initials: B
authors.lastname: Giannesini
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena / Q000187:drug effects*; D002211:Capsaicin / Q000008:administration & dosage / Q000031:analogs & derivatives*; D002478:Cells, Cultured; D004558:Electric Stimulation; D004734:Energy Metabolism / Q000187:drug effects*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009119:Muscle Contraction / Q000187:drug effects* / Q000502:physiology; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000378:metabolism; D010805:Physical Conditioning, Animal / Q000502:physiology
chemical_list: C470213:capsiate; D002211:Capsaicin
keywords: capsinoids; ergogenic effect; muscle fatigue; uncoupling protein; vanilloid receptors