title: mga genosensor for early detection of human rheumatic heart disease.
abstract: The 5' amino-labeled DNA probe complementary to mga gene of Streptococcus pyogenes was immobilized on carboxylated multiwall carbon nanotubes electrode and hybridized with 0.1-100 ng/6 μl single-stranded genomic DNA (ssG-DNA) of S. pyogenes from throat swab of suspected rheumatic heart disease (RHD) patients. Electrochemical response was measured by cyclic voltammetry (CV), differential pulse voltammetry (DPV), and electrochemical impedance (EI). The sensitivity of the sensor was 106.03 (μA/cm(2))/ng and limit of detection (LOD) was found 0.014 ng/6 μl with regression coefficient (R(2)) of 0.921 using DPV. The genosensor was characterized by FTIR and SEM, and electrode was found stable for 6 months on storage at 4 °C with 5-6 % loss in initial DPV current. mga genosensor is the first report on RHD sensor which can save life of several suspected patients by early diagnosis in 30 min.
authors.affiliation: CSIR-Institute of Genomics and Integrative Biology, Mall Road, Delhi, 110007, India.
authors.forename: Swati
authors.initials: S
authors.lastname: Singh
authors.forename: Ankur
authors.initials: A
authors.lastname: Kaushal
authors.forename: Shashi
authors.initials: S
authors.lastname: Khare
authors.forename: Ashok
authors.initials: A
authors.lastname: Kumar
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis* / Q000235:genetics; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D042241:Early Diagnosis; D006801:Humans; D010614:Pharynx / Q000382:microbiology; D012214:Rheumatic Heart Disease / Q000175:diagnosis* / Q000382:microbiology; D013297:Streptococcus pyogenes / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; C068267:mry protein, Streptococcus pyogenes