title: Inflammatory responses and side effects generated by several adjuvant-containing vaccines in turbot.
abstract: Several of the adjuvants used in fish vaccines cause adhesions in internal organs when they are injected intraperitoneally. We describe the damage caused by vaccines containing different adjuvants in the turbot Scophthalmus maximus and show that internal adhesions can be greatly reduced by injecting the fish in a specific way. Injection of fish with the needle directed towards the anterior part of the peritoneal cavity induced formation of a single cell-vaccine mass (CVM) that became attached to the parietal peritoneum. However, injection of the fish with the needle pointing in the opposite direction generated many small CVM that became attached to the visceral and parietal peritoneum and in some cases caused internal adhesions. We describe the structural and cellular changes in the adjuvant-induced CVMs. The CVMs mainly comprised neutrophils and macrophages, although most of the former underwent apoptosis, which was particularly evident from day 3 post-injection. The apoptotic cells were phagocytosed by macrophages, which were the dominant cell type from the first days onwards. All of the vaccines induced angiogenesis in the area of contact between the CVM and the mesothelium. Vaccines containing oil-based adjuvants or microspheres induced the formation of granulomas in the CVM; however, no granulomas were observed in the CVM induced by vaccines containing aluminium hydroxide or Matrix-Q(®) as adjuvants. All of the vaccines induced strong migration of cells to the peritoneal cavity. Although some of these cells remained unattached in the peritoneal cavity, most of them formed part of the CVM. We also observed migration of the cells from the peritoneal cavity to lymphoid organs, indicating bidirectional traffic of cells between the inflamed areas and these organs.
authors.affiliation: Departamento de Biología Celular y Ecología, Universidad de Santiago de Compostela, 15782 Santiago de Compostela, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Noia
authors.affiliation: Departamento de Biología Celular y Ecología, Universidad de Santiago de Compostela, 15782 Santiago de Compostela, Spain.
authors.forename: B
authors.initials: B
authors.lastname: Domínguez
authors.affiliation: Laboratorio de Parasitología, Instituto de Investigación y Análisis Alimentarios, Universidad de Santiago de Compostela, 15782 Santiago de Compostela, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Leiro
authors.affiliation: Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Ciencias, Universidad de Santiago de Compostela, 27002 Lugo, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Blanco-Méndez
authors.affiliation: Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Ciencias, Universidad de Santiago de Compostela, 27002 Lugo, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Luzardo-Álvarez
authors.affiliation: Departamento de Biología Celular y Ecología, Universidad de Santiago de Compostela, 15782 Santiago de Compostela, Spain. Electronic address: jesus.lamas@usc.es.
authors.forename: J
authors.initials: J
authors.lastname: Lamas
mesh_terms: D000276:Adjuvants, Immunologic / Q000009:adverse effects*; D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D005393:Fish Diseases / Q000139:chemically induced* / Q000473:pathology; D005412:Flatfishes; D007249:Inflammation / Q000139:chemically induced / Q000662:veterinary*; D000267:Tissue Adhesions / Q000139:chemically induced / Q000662:veterinary; D014612:Vaccines / Q000008:administration & dosage / Q000009:adverse effects*
chemical_list: D000276:Adjuvants, Immunologic; D014612:Vaccines
keywords: Adjuvant; Fish; Inflammatory response; Side effects; Vaccine