title: In search of ligands and receptors of the pollen tube: the missing link in pollen tube perception.
abstract: The journey undertaken by the pollen tube in angiosperms to reach the deeply embedded female gametophyte for fertilization involves persistent guidance by the female gametophyte and accurate perception of the signals by the pollen tube. Several ovule-secreted peptides have been identified. Nevertheless, there are no exact findings on how these signals are perceived by the pollen tube. As a novel approach, we have improvised a modified SIV (semi-in vivo) technique, SIV-PS (SIV pollen tube secretome), to perform gel-free LC-MS/MS for high-throughput analysis of pollen-tube-secreted proteins. Our approach has led to the identification of over 1400 protein groups. Among them are pollen-tube-secreted ligands and receptor proteins representing potential male components in perceiving ovule-emitted cues for guidance. The present article reviews the missing link in pollen tube perception and showcases the improvised SIV-PS as a tool for high-throughput and targeted study of the pollen tube secretome.
authors.affiliation: *Laboratory of Pollen Biology, Institute of Experimental Botany ASCR, Rozvojová 263, 165 02 Praha 6, Czech Republic.
authors.forename: Said
authors.initials: S
authors.lastname: Hafidh
authors.forename: David
authors.initials: D
authors.lastname: Potěšil
authors.affiliation: *Laboratory of Pollen Biology, Institute of Experimental Botany ASCR, Rozvojová 263, 165 02 Praha 6, Czech Republic.
authors.forename: Jan
authors.initials: J
authors.lastname: Fíla
authors.affiliation: *Laboratory of Pollen Biology, Institute of Experimental Botany ASCR, Rozvojová 263, 165 02 Praha 6, Czech Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Feciková
authors.affiliation: *Laboratory of Pollen Biology, Institute of Experimental Botany ASCR, Rozvojová 263, 165 02 Praha 6, Czech Republic.
authors.forename: Věra
authors.initials: V
authors.lastname: Čapková
authors.forename: Zbyněk
authors.initials: Z
authors.lastname: Zdráhal
authors.affiliation: *Laboratory of Pollen Biology, Institute of Experimental Botany ASCR, Rozvojová 263, 165 02 Praha 6, Czech Republic.
authors.forename: David
authors.initials: D
authors.lastname: Honys
mesh_terms: D002633:Chemotaxis / Q000502:physiology; D008024:Ligands; D010944:Plants / Q000378:metabolism; D053205:Pollen Tube / Q000378:metabolism* / Q000502:physiology*; D015398:Signal Transduction / Q000502:physiology
chemical_list: D008024:Ligands