title: The cohesin acetyltransferase Eco1 coordinates rDNA replication and transcription.
abstract: Eco1 is the acetyltransferase that establishes sister-chromatid cohesion during DNA replication. A budding yeast strain with an eco1 mutation that genocopies Roberts syndrome has reduced ribosomal DNA (rDNA) transcription and a transcriptional signature of starvation. We show that deleting FOB1--a gene that encodes a replication fork-blocking protein specific for the rDNA region--rescues rRNA production and partially rescues transcription genome-wide. Further studies show that deletion of FOB1 corrects the genome-wide replication defects, nucleolar structure, and rDNA segregation that occur in the eco1 mutant. Our study highlights that the presence of cohesin at the rDNA locus has a central role in controlling global DNA replication and gene expression.
authors.affiliation: Stowers Institute for Medical Research, Kansas City, MO, USA.
authors.forename: Shuai
authors.initials: S
authors.lastname: Lu
authors.forename: Kenneth K
authors.initials: KK
authors.lastname: Lee
authors.forename: Bethany
authors.initials: B
authors.lastname: Harris
authors.forename: Bo
authors.initials: B
authors.lastname: Xiong
authors.forename: Tania
authors.initials: T
authors.lastname: Bose
authors.forename: Anita
authors.initials: A
authors.lastname: Saraf
authors.forename: Gaye
authors.initials: G
authors.lastname: Hattem
authors.forename: Laurence
authors.initials: L
authors.lastname: Florens
authors.forename: Chris
authors.initials: C
authors.lastname: Seidel
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Gerton
mesh_terms: D000123:Acetyltransferases / Q000235:genetics*; D018797:Cell Cycle Proteins / Q000378:metabolism; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism; D004261:DNA Replication / Q000235:genetics*; D004271:DNA, Fungal / Q000235:genetics; D004275:DNA, Ribosomal / Q000096:biosynthesis* / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D017353:Gene Deletion; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics*; D012335:RNA, Ribosomal / Q000235:genetics; D012270:Ribosomes / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*; D014158:Transcription, Genetic / Q000235:genetics; D000097722:Cohesins
chemical_list: D000123:Acetyltransferases; D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D004271:DNA, Fungal; D004275:DNA, Ribosomal; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D012335:RNA, Ribosomal; D029701:Saccharomyces cerevisiae Proteins; D000097722:Cohesins; C105579:FOB1 protein, S cerevisiae; C118076:ECO1 protein, S cerevisiae