title: Multilayer mounting for long-term light sheet microscopy of zebrafish.
abstract: Light sheet microscopy is the ideal imaging technique to study zebrafish embryonic development. Due to minimal photo-toxicity and bleaching, it is particularly suited for long-term time-lapse imaging over many hours up to several days. However, an appropriate sample mounting strategy is needed that offers both confinement and normal development of the sample. Multilayer mounting, a new embedding technique using low-concentration agarose in optically clear tubes, now overcomes this limitation and unleashes the full potential of light sheet microscopy for real-time developmental biology.
authors.affiliation: Huisken Lab, Max Planck Institute of Molecular Cell Biology and Genetics.
authors.forename: Michael
authors.initials: M
authors.lastname: Weber
authors.affiliation: Huisken Lab, Max Planck Institute of Molecular Cell Biology and Genetics.
authors.forename: Michaela
authors.initials: M
authors.lastname: Mickoleit
authors.affiliation: Huisken Lab, Max Planck Institute of Molecular Cell Biology and Genetics; huisken@mpi-cbg.de.
authors.forename: Jan
authors.initials: J
authors.lastname: Huisken
mesh_terms: D000818:Animals; D005260:Female; D008297:Male; D008853:Microscopy / Q000295:instrumentation* / Q000379:methods*; D012685:Sepharose; D059008:Time-Lapse Imaging / Q000295:instrumentation* / Q000379:methods*; D015027:Zebrafish / Q000196:embryology*
chemical_list: D012685:Sepharose