title: Role of vitamin D derivatives in intestinal tissue of patients with inflammatory bowel diseases.
abstract: OBJECTIVE
  The adhesion molecule expression and matrix metalloproteinases (MMPs) are proposed to be major factors for intestinal injury mediated by T cells in (IBD) and are up-regulated in intestinal mucosa of IBD patients. To investigate the effect of vitamin D derivatives on adhesion molecules and MMPs in colonic biopsies of IBD patients.
  
  
  METHODS
  Biopsies from inflamed and non-inflamed tract of terminal ileum and colon and PBMC from the same IBD patients were cultured with or without vitamin D derivatives. MMP activity and adhesion molecule levels were determined.
  
  
  RESULTS
  1,25(OH)2D3 and ZK 191784 significantly decrease ICAM-1 protein levels in the biopsies obtained only from the inflamed region of intestine of UC patients, while MAdCAM-1 levels decrease in the presence of 1,25(OH)2D3 in the non-inflamed region, and, in the presence of ZK, in the inflamed one. In CD patients 1,25(OH)2D3 and ZK decrease ICAM-1 and MAdCAM-1 in the biopsies obtained from the non-inflamed and inflamed regions, with the exception of ICAM-1 in the inflamed region in the presence of 1,25(OH)2D3. The expression of MMP-9, MMP-2, and MMP-3 decreases in the presence of vitamin D derivatives in UC and CD with the exception of 1,25(OH)2D3 that does not affect the levels of MMP-9 and MMP-2 in CD. Vitamin D derivatives always affect MMP-9, MMP-2 and ICAM-1 in PBMC of UC and CD patients.
  
  
  CONCLUSIONS
  Based on the increased expression of ICAM-1, MAdCAM-1 and MMP-2,-9,-3 in IBD, our study suggests that vitamin D derivatives may be effective in the management of these diseases.
authors.affiliation: Department of Biomedical, Experimental and Clinical Sciences, University of Florence, Viale Morgagni 50, 50134 Florence, Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Martinesi
authors.affiliation: Department of Biomedical, Experimental and Clinical Sciences, University of Florence, Viale Morgagni 50, 50134 Florence, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Ambrosini
authors.affiliation: Department of Biomedical, Experimental and Clinical Sciences, University of Florence, Viale Morgagni 50, 50134 Florence, Italy.
authors.forename: Cristina
authors.initials: C
authors.lastname: Treves
authors.affiliation: Clinical Sciences, Global Biomarker, Global Discovery, Bayer Healthcare, Bayer, 10178 Berlin, Germany.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Zuegel
authors.affiliation: Medicinal Chemistry, Global Drug Discovery, Bayer Healthcare, Bayer, 10178 Berlin, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Steinmeyer
authors.affiliation: Division of Gastroenterology 2, Careggi Hospital, 50134 Florence, Italy.
authors.forename: Annese
authors.initials: A
authors.lastname: Vito
authors.affiliation: Regional Referral Center for IBD, Careggi Hospital, 50134 Florence, Italy.
authors.forename: Monica
authors.initials: M
authors.lastname: Milla
authors.affiliation: Division of Gastroenterology 2, Careggi Hospital, 50134 Florence, Italy.
authors.forename: Andrea G
authors.initials: AG
authors.lastname: Bonanomi
authors.affiliation: Department of Biomedical, Experimental and Clinical Sciences, University of Florence, Viale Morgagni 50, 50134 Florence, Italy. Electronic address: maria.stio@unifi.it.
authors.forename: Maria
authors.initials: M
authors.lastname: Stio
mesh_terms: D001706:Biopsy; D015153:Blotting, Western; D002117:Calcitriol / Q000031:analogs & derivatives* / Q000627:therapeutic use; D015815:Cell Adhesion Molecules; D002478:Cells, Cultured; D006801:Humans; D006887:Hydroxycholecalciferols / Q000627:therapeutic use*; D007136:Immunoglobulins / Q000187:drug effects / Q000378:metabolism*; D015212:Inflammatory Bowel Diseases / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D018799:Intercellular Adhesion Molecule-1 / Q000187:drug effects / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D020782:Matrix Metalloproteinases / Q000187:drug effects / Q000378:metabolism*; D009088:Mucoproteins / Q000187:drug effects / Q000378:metabolism*; D014807:Vitamin D / Q000031:analogs & derivatives; D014815:Vitamins
chemical_list: D015815:Cell Adhesion Molecules; D006887:Hydroxycholecalciferols; D007136:Immunoglobulins; C504265:MADCAM1 protein, human; D009088:Mucoproteins; D014815:Vitamins; C470666:ZK 191784; D018799:Intercellular Adhesion Molecule-1; D014807:Vitamin D; C030049:1,25,26-trihydroxyvitamin D3; D020782:Matrix Metalloproteinases; D002117:Calcitriol
keywords: Adhesion molecules; Inflammatory bowel disease; Matrix metalloproteinase; Vitamin D derivatives