title: Improving Method Reliability in Carotenoid Analysis through Selective Removal of Glycerolipid Interferences by Lipase Treatment.
abstract: Saponification is most often used to hydrolyze glycerolipid interferences during carotenoid analysis. Ester bonds of other plant metabolites such as carotenoids are, however, also hydrolyzed during saponification, thus altering the natural carotenoid composition. A straightforward and selective cleanup procedure was therefore developed involving the enzymatic hydrolysis of matrix glycerolipids. The optimized procedure (100 μL of extracted vegetable or algal oil in 20 mL of 50:50 phosphate buffer/methanol with 25 μL of sodium n-octyl sulfate, 30 mg of bile salts, and 250 μL of NaCl solution (5 mM), magnetic stirring for 2 h at 40 °C with 1 mL of Lipozyme TL 100 L and 1 mL of Lipozyme CALB L) removed the greater part of triglycerides (94.8-100.0%) and diglycerides (88.2-99.8%) while preserving the natural carotenoid composition.
authors.affiliation: Business Unit Separation and Conversion Technology (SCT), Flemish Institute for Technological Research (VITO) , Boeretang 200, 2400 Mol, Belgium.|NatuRA, University of Antwerp , Universiteitsplein 1, 2610 Antwerp, Belgium.
authors.forename: Sebastiaan
authors.initials: S
authors.lastname: Bijttebier
authors.affiliation: Business Unit Separation and Conversion Technology (SCT), Flemish Institute for Technological Research (VITO) , Boeretang 200, 2400 Mol, Belgium.
authors.forename: Els
authors.initials: E
authors.lastname: D'Hondt
authors.affiliation: Business Unit Separation and Conversion Technology (SCT), Flemish Institute for Technological Research (VITO) , Boeretang 200, 2400 Mol, Belgium.
authors.forename: Bart
authors.initials: B
authors.lastname: Noten
authors.affiliation: NatuRA, University of Antwerp , Universiteitsplein 1, 2610 Antwerp, Belgium.
authors.forename: Nina
authors.initials: N
authors.lastname: Hermans
authors.affiliation: NatuRA, University of Antwerp , Universiteitsplein 1, 2610 Antwerp, Belgium.
authors.forename: Sandra
authors.initials: S
authors.lastname: Apers
authors.affiliation: Business Unit Separation and Conversion Technology (SCT), Flemish Institute for Technological Research (VITO) , Boeretang 200, 2400 Mol, Belgium.
authors.forename: Stefan
authors.initials: S
authors.lastname: Voorspoels
keywords: carotenoid LC-MS analysis; esterified carotenoids; ionization suppression; lipase treatment; selective glycerolipid hydrolysis