title: Rapid and sensitive screening of 17β-estradiol estrogenicity using Fourier transform infrared imaging spectroscopy (FT-IRIS).
abstract: It is important to develop rapid and sensitive screening assays to assess the biological effects of emerging contaminants. In this contribution, the ability to determine the molecular level effects of 17β-estradiol on single MCF-7 cells using Fourier transform infrared imaging spectroscopy (FT-IRIS) was investigated. The use of FT-IRIS enabled subcellular imaging of the cells and determination of a dose dependent response in mucin concentration at 24 and 48 h of incubation. The 48 h increase in mucin was comparable to increases in cellular proliferation (Pearson R = 0.978). The EC50 values for the E-screen and FT-IRIS assays were 2.29 and 2.56 ppt, respectively, indicating that the molecular changes, which are observed at the single cell level using FT-IRIS, are reflective of physiological changes that are observed as the cell population responds to 17ß-estradiol. The FT-IRIS method, when combined with principal component analysis, enabled differentiation and grouping of cells exposed to varying concentrations of 17ß-estradiol. The FT-IRIS method shows potential to be used as a rapid and sensitive screening technique for the detection of biological responses to different emerging contaminants in relevant cells or tissues.
authors.affiliation: NSF Water & Environmental Technology (WET) Center, Department of Civil and Environmental Engineering, Temple University , Philadelphia, Pennsylvania 19122, United States.
authors.forename: Candice M
authors.initials: CM
authors.lastname: Johnson
authors.forename: Nancy
authors.initials: N
authors.lastname: Pleshko
authors.forename: Mohan
authors.initials: M
authors.lastname: Achary
authors.forename: Rominder P S
authors.initials: RP
authors.lastname: Suri
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D004958:Estradiol / Q000032:analysis* / Q000494:pharmacology*; D006801:Humans; D061986:MCF-7 Cells; D025341:Principal Component Analysis; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D013347:Subcellular Fractions / Q000187:drug effects / Q000378:metabolism
chemical_list: D004958:Estradiol