title: A growth quantification assay for Hyaloperonospora arabidopsidis isolates in Arabidopsis thaliana.
abstract: There is a considerable interest in determining the role of individual oomycete effectors in promoting disease. Widely used strategies are based on manipulating effector-expression levels in the pathogen and by over-expressing particular effectors in the host by genetic transformation. In the case of the oomycete, Hyaloperonospora arabidopsidis (Hpa) genetic manipulation is not yet possible, so over-expression of predicted effectors in stably transformed Arabidopsis lines is used to investigate their capability for promoting virulence. Here, we describe a technique for quantifying pathogen growth based on the counting of asexual reproductive structures called sporangiophores in the compatible interaction between the Hpa isolate Noks1 and the Col-0 Arabidopsis accession.
authors.affiliation: School of Life Sciences, Warwick University, Gibbet Hill Campus, Coventry, CV4 7 AL, United Kingdom.
authors.forename: Daniel F A
authors.initials: DF
authors.lastname: Tomé
authors.forename: Jens
authors.initials: J
authors.lastname: Steinbrenner
authors.forename: Jim L
authors.initials: JL
authors.lastname: Beynon
mesh_terms: D017360:Arabidopsis / Q000382:microbiology*; D001681:Biological Assay / Q000379:methods*; D002478:Cells, Cultured; D009868:Oomycetes / Q000302:isolation & purification*; D030821:Plants, Genetically Modified; D013170:Spores / Q000502:physiology