title: Caprine arthritis encephalitis virus detection in blood by loop-mediated isothermal amplification (LAMP) assay targeting the proviral gag region.
abstract: Caprine arthritis encephalitis virus (CAEV), of the genus Lentivirus of the Retroviridae family, causes persistent disease, which is characterized by polyarthritis and mastitis in adult goats and progressive paresis (leukoencephalomyelitis) in kids. A loop-mediated isothermal amplification (LAMP) assay was developed for the detection of CAEV in blood samples. Species-specific primers amplifying the gag gene region in the provirus were used for the detection of CAEV. The LAMP assay result was obtained 30 min after incubation on a constant temperature at 63 °C in a heat block. Resulting amplicons were visualized by addition of SYBR green dye after the reaction and checked by agarose gel electrophoresis. The sensitivity of LAMP assay was evaluated by comparing the result with the nested polymerase chain reaction. Based on the experiments, the result of the assay indicated a rapid and sensitive test for the detection of CAEV.
authors.affiliation: Animal Health Unit, Philippine Carabao Center National Headquarters and Gene Pool, Science City of Munoz 3120, Nueva Ecija, Philippines.
authors.forename: Michelle M
authors.initials: MM
authors.lastname: Balbin
authors.affiliation: Animal Health Unit, Philippine Carabao Center National Headquarters and Gene Pool, Science City of Munoz 3120, Nueva Ecija, Philippines.
authors.forename: Lawrence P
authors.initials: LP
authors.lastname: Belotindos
authors.affiliation: Animal Health Unit, Philippine Carabao Center National Headquarters and Gene Pool, Science City of Munoz 3120, Nueva Ecija, Philippines.
authors.forename: Nancy S
authors.initials: NS
authors.lastname: Abes
authors.affiliation: Animal Health Unit, Philippine Carabao Center National Headquarters and Gene Pool, Science City of Munoz 3120, Nueva Ecija, Philippines. Electronic address: cnmingala@hotmail.com.
authors.forename: Claro N
authors.initials: CN
authors.lastname: Mingala
mesh_terms: D000818:Animals; D016088:Arthritis-Encephalitis Virus, Caprine / Q000235:genetics* / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D015683:Gene Products, gag / Q000235:genetics*; D006041:Goats; D016180:Lentivirus Infections / Q000175:diagnosis / Q000821:virology*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*
chemical_list: D015683:Gene Products, gag
keywords: Caprine arthritis encephalitis virus; Gag gene region; Loop-mediated isothermal amplification; Nested-PCR