title: [Preparation and performance assessment of Gamma-peptide nucleic acid gene chip detection system based on surface plasmon resonance].
abstract: The aim of this study was to build a gene chip system with surface plasmon resonance (SPR) technique, for which Gamma-peptide nucleic acid (Gamma-PNA) functioned as a probe, in order to improve sensitivity and its specificity. With the use of self-assembled monolayer (SAM) technology, surface chemistry of two-dimensional structure was used. Gamma-PNA was designed according to the bioinformatics, and was plated on the SPR chip modified by SAM. Subsequently, relevant parameters of the experiment were ensured and optimized. The results showed that the performances of Gamma-PNA probe was little affected by the ion concentration of buffer, and it had a strong light signal in a stable state. As the ion concentration was 0, there were still good hybrid reactions; pH value had less influence upon Gamma-PNA probe, and acid environment of buffer could be better. Gamma-PNA probe combined with sensor technologies achieved made the probe with dispensable labels and real-time detection. It also improved the efficiency of the hybridization and the stability, providing the foundation for clinical application.
authors.forename: Qingye
authors.initials: Q
authors.lastname: Ou
authors.forename: Dayong
authors.initials: D
authors.lastname: Gu
authors.forename: Niqi
authors.initials: N
authors.lastname: Zhang
authors.forename: Jian'an
authors.initials: J
authors.lastname: He
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Shao
authors.forename: Lei
authors.initials: L
authors.lastname: Shi
authors.forename: Chunxiao
authors.initials: C
authors.lastname: Liu
authors.forename: Chunzhong
authors.initials: C
authors.lastname: Zhao
authors.forename: Yunqing
authors.initials: Y
authors.lastname: Xu
mesh_terms: D009693:Nucleic Acid Hybridization; D015341:Nucleic Acid Probes; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D020135:Peptide Nucleic Acids / Q000235:genetics*; D012680:Sensitivity and Specificity; D020349:Surface Plasmon Resonance
chemical_list: D015341:Nucleic Acid Probes; D020135:Peptide Nucleic Acids