title: MiR-129-5p is down-regulated and involved in the growth, apoptosis and migration of medullary thyroid carcinoma cells through targeting RET.
abstract: Dysregulation of the REarranged during Transfection proto-oncogene (RET) pathway and microRNA (miRNAs) are crucial for the development of medullary thyroid carcinomas (MTC). Here we demonstrate that miR-129-5p is down-regulated in MTC tissues and cell lines and inhibits RET expression by directly binding its 3' untranslated regions. Ectopic expression of miR-129-5p significantly decreases cell growth, induces apoptosis and suppresses migration ability in MTC cells through decreasing the phosphorylated AKT, thus functioning as a tumor suppressor. These findings give new clues for understanding MTC carcinogenesis and may help in developing a therapeutic approach for the treatment of RET-activated MTC.
authors.affiliation: Department of Endocrinology, Tianjin First Central Hospital, No. 24 Fu Kang Road, Tianjin 300192, China. Electronic address: duanlijun1102@163.com.
authors.forename: Lijun
authors.initials: L
authors.lastname: Duan
authors.affiliation: School of Basic Medical Sciences, Hebei United University, Tangshan City, Hebei Province 063000, China.
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Hao
authors.affiliation: Hebei United University Affiliated Hospital, Tangshan City, Hebei Province 063000, China.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Endocrinology, Tianjin First Central Hospital, No. 24 Fu Kang Road, Tianjin 300192, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: School of Basic Medical Sciences, Hebei United University, Tangshan City, Hebei Province 063000, China. Electronic address: zhguangling@126.com.
authors.forename: Guangling
authors.initials: G
authors.lastname: Zhang
mesh_terms: D020413:3' Untranslated Regions; D000818:Animals; D017209:Apoptosis; D001483:Base Sequence; D001665:Binding Sites; D018278:Carcinoma, Neuroendocrine; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D002470:Cell Survival; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D009368:Neoplasm Transplantation; D000090063:Proto-Oncogene Mas; D051096:Proto-Oncogene Proteins c-ret / Q000235:genetics* / Q000378:metabolism; D034622:RNA Interference; D013964:Thyroid Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D047368:Tumor Burden
chemical_list: D020413:3' Untranslated Regions; C000715470:MAS1 protein, human; D035683:MicroRNAs; C541915:Mirn129 microRNA, human; D000090063:Proto-Oncogene Mas; D051096:Proto-Oncogene Proteins c-ret; C099282:RET protein, human
keywords: Cell apoptosis; Cell growth; Cell migration; Medullary thyroid carcinoma; MiR-129-5p; REarranged during Transfection proto-oncogene