title: Chitosan nanoparticles as a potential nonviral gene delivery for HPV-16 E7 into mammalian cells.
abstract: Chitosan nanoparticles (CS NPs) were prepared as a carrier for Human papillomavirus type 16 HPV-16) E7 gene and their gene transfection ability were evaluated in vitro. The plasmid expressing green fluorescent protein (pEGFP) was used as a reporter gene. Gel electrophoresis demonstrated full binding of CS NPs with the pDNA. The transfection of CS-pEGFP NPs was efficient in CHO cells and the expression of green fluorescent proteins was well observed. The expression of E7 proteins was confirmed under SDS-PAGE and western blot analysis. As a conclusion CS NPs may serve as an effective nonviral carrier for delivery of nucleotides into eukaryotic cells.
authors.affiliation: a Department of Microbiology , Infectious Diseases Research Center, Golestan University of Medical Sciences , Gorgan , Iran.|b Department of Virology , School of Public Health, Tehran University of Medical Sciences , Tehran , Iran.
authors.forename: Alireza
authors.initials: A
authors.lastname: Tahamtan
authors.affiliation: a Department of Microbiology , Infectious Diseases Research Center, Golestan University of Medical Sciences , Gorgan , Iran.
authors.forename: Alijan
authors.initials: A
authors.lastname: Tabarraei
authors.affiliation: a Department of Microbiology , Infectious Diseases Research Center, Golestan University of Medical Sciences , Gorgan , Iran.
authors.forename: Abdolvahab
authors.initials: A
authors.lastname: Moradi
authors.affiliation: c Department of Pharmaceutical Nanotechnology , Faculty of Pharmacy, Tehran University of Medical Sciences , Tehran , Iran.
authors.forename: Meshkat
authors.initials: M
authors.lastname: Dinarvand
authors.affiliation: a Department of Microbiology , Infectious Diseases Research Center, Golestan University of Medical Sciences , Gorgan , Iran.
authors.forename: Mishar
authors.initials: M
authors.lastname: Kelishadi
authors.affiliation: a Department of Microbiology , Infectious Diseases Research Center, Golestan University of Medical Sciences , Gorgan , Iran.
authors.forename: Amir
authors.initials: A
authors.lastname: Ghaemi
authors.affiliation: d Nanotechnology Research Centre, Faculty of Pharmacy, Tehran University of Medical Sciences , Tehran , Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Atyabi
mesh_terms: D000818:Animals; D015153:Blotting, Western; D016466:CHO Cells; D048271:Chitosan / Q000737:chemistry / Q000378:metabolism*; D003412:Cricetulus; D004591:Electrophoresis, Polyacrylamide Gel; D015870:Gene Expression; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D052162:Human papillomavirus 16 / Q000737:chemistry / Q000235:genetics; D053758:Nanoparticles / Q000737:chemistry / Q000378:metabolism*; D050725:Papillomavirus E7 Proteins / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism
chemical_list: D050725:Papillomavirus E7 Proteins; C485184:enhanced green fluorescent protein; C059731:oncogene protein E7, Human papillomavirus type 16; D049452:Green Fluorescent Proteins; D048271:Chitosan
keywords: CHO cell line; HPV-16 E7; chitosan NPs; gene delivery