title: Jumonji/Arid1b (Jarid1b) protein modulates human esophageal cancer cell growth.
abstract: Although esophageal cancer is highly heterogeneous and the involvement of epigenetic regulation of cancer stem cells is highly suspected, the biological significance of epigenetically modified molecules that regulate different subpopulations remains to be firmly established. Using esophageal cancer cells, we investigated the functional roles of the H3K4 demethylase Jumonji/Arid1b (Jarid1b) (Kdm5b/Plu-1/Rbp2-h1), an epigenetic factor that is required for continuous cell growth in melanoma.JARID1Bknockdown resulted in the suppression of esophageal cancer cell growth, sphere formation and invasion ability and was associated with loss of epithelial marker expression. However, these inhibitory effects observed on tumor formation were reverted subsequent to subcutaneous inoculation of these cells into immune-deficient mice. These results indicated thatJARID1Bplays a role in maintaining cancer stem cells in the esophagus and justifies the rationale for studying the effects of continuous inhibition of this epigenetic factor in esophageal cancer.
authors.affiliation: Departments of Frontier Science for Cancer and Chemotherapy, Osaka University, Graduate School of Medicine, Suita, Osaka 565-0871; ; Gastroenterological Surgery, Osaka University, Graduate School of Medicine, Suita, Osaka 565-0871;
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Kano
authors.affiliation: Departments of Frontier Science for Cancer and Chemotherapy, Osaka University, Graduate School of Medicine, Suita, Osaka 565-0871;
authors.forename: Masamitsu
authors.initials: M
authors.lastname: Konno
authors.affiliation: Departments of Frontier Science for Cancer and Chemotherapy, Osaka University, Graduate School of Medicine, Suita, Osaka 565-0871; ; Gastroenterological Surgery, Osaka University, Graduate School of Medicine, Suita, Osaka 565-0871;
authors.forename: Katsuya
authors.initials: K
authors.lastname: Ohta
authors.affiliation: Gastroenterological Surgery, Osaka University, Graduate School of Medicine, Suita, Osaka 565-0871;
authors.forename: Naotsugu
authors.initials: N
authors.lastname: Haraguchi
authors.affiliation: Departments of Frontier Science for Cancer and Chemotherapy, Osaka University, Graduate School of Medicine, Suita, Osaka 565-0871; ; Gastroenterological Surgery, Osaka University, Graduate School of Medicine, Suita, Osaka 565-0871;
authors.forename: Shimpei
authors.initials: S
authors.lastname: Nishikawa
authors.affiliation: Gastroenterological Surgery, Osaka University, Graduate School of Medicine, Suita, Osaka 565-0871;
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Kagawa
authors.affiliation: Departments of Frontier Science for Cancer and Chemotherapy, Osaka University, Graduate School of Medicine, Suita, Osaka 565-0871; ; Gastroenterological Surgery, Osaka University, Graduate School of Medicine, Suita, Osaka 565-0871;
authors.forename: Atsushi
authors.initials: A
authors.lastname: Hamabe
authors.affiliation: Departments of Frontier Science for Cancer and Chemotherapy, Osaka University, Graduate School of Medicine, Suita, Osaka 565-0871; ; Gastroenterological Surgery, Osaka University, Graduate School of Medicine, Suita, Osaka 565-0871;
authors.forename: Shinichiro
authors.initials: S
authors.lastname: Hasegawa
authors.affiliation: Departments of Frontier Science for Cancer and Chemotherapy, Osaka University, Graduate School of Medicine, Suita, Osaka 565-0871; ; Gastroenterological Surgery, Osaka University, Graduate School of Medicine, Suita, Osaka 565-0871;
authors.forename: Hisataka
authors.initials: H
authors.lastname: Ogawa
authors.affiliation: Departments of Frontier Science for Cancer and Chemotherapy, Osaka University, Graduate School of Medicine, Suita, Osaka 565-0871; ; Otorhinolaryngology-Head and Neck Surgery, Osaka University, Graduate School of Medicine, Suita, Osaka 565-0871;
authors.forename: Takahito
authors.initials: T
authors.lastname: Fukusumi
authors.affiliation: Departments of Frontier Science for Cancer and Chemotherapy, Osaka University, Graduate School of Medicine, Suita, Osaka 565-0871;
authors.forename: Yuko
authors.initials: Y
authors.lastname: Noguchi
authors.affiliation: Departments of Frontier Science for Cancer and Chemotherapy, Osaka University, Graduate School of Medicine, Suita, Osaka 565-0871; ; Gastroenterological Surgery, Osaka University, Graduate School of Medicine, Suita, Osaka 565-0871;
authors.forename: Miyuki
authors.initials: M
authors.lastname: Ozaki
authors.affiliation: Departments of Frontier Science for Cancer and Chemotherapy, Osaka University, Graduate School of Medicine, Suita, Osaka 565-0871;
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Kudo
authors.affiliation: Departments of Frontier Science for Cancer and Chemotherapy, Osaka University, Graduate School of Medicine, Suita, Osaka 565-0871;
authors.forename: Daisuke
authors.initials: D
authors.lastname: Sakai
authors.affiliation: Departments of Frontier Science for Cancer and Chemotherapy, Osaka University, Graduate School of Medicine, Suita, Osaka 565-0871;
authors.forename: Taroh
authors.initials: T
authors.lastname: Satoh
authors.affiliation: Laboratory of Cellular Dynamics, WPI-Immunology Frontier Research Center, Osaka University, Suita, Osaka 565-0871; ; Japan Science and Technology Agency, Core Research for Evolutional Science and Technology, Chiyoda, Tokyo 102-0075;
authors.forename: Masaru
authors.initials: M
authors.lastname: Ishii
authors.affiliation: Osaka University, Graduate School of Technology, Suita, Osaka 565-0871, Japan.
authors.forename: Eiichi
authors.initials: E
authors.lastname: Mizohata
authors.affiliation: Osaka University, Graduate School of Technology, Suita, Osaka 565-0871, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Inoue
authors.affiliation: Gastroenterological Surgery, Osaka University, Graduate School of Medicine, Suita, Osaka 565-0871;
authors.forename: Masaki
authors.initials: M
authors.lastname: Mori
authors.affiliation: Gastroenterological Surgery, Osaka University, Graduate School of Medicine, Suita, Osaka 565-0871;
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Doki
authors.affiliation: Departments of Frontier Science for Cancer and Chemotherapy, Osaka University, Graduate School of Medicine, Suita, Osaka 565-0871;
authors.forename: Hideshi
authors.initials: H
authors.lastname: Ishii
keywords: Jumonji/Arid1B; esophageal cancer; gastrointestinal organs