title: Direct potentiometric quantification of histamine using solid-phase imprinted nanoparticles as recognition elements.
abstract: A new potentiometric sensor based on molecularly imprinted nanoparticles produced via the solid-phase imprinting method was developed. For histamine quantification, the nanoparticles were incorporated within a membrane, which was then used to fabricate an ion-selective electrode. The use of nanoparticles with high affinity and specificity allowed for label-free detection/quantification of histamine in real samples with short response times. The sensor could selectively quantify histamine in presence of other biogenic amines in real wine and fish matrices. The limit of detection achieved was 1.12×10(-6)molL(-1), with a linear range between 10(-6) and 10(-2)molL(-1) and a response time below 20s, making the sensor as developed a promising tool for direct quantification of histamine in the food industry.
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmacy, University of the Basque Country, 01006 Vitoria-Gasteiz, Spain.
authors.forename: Itsaso
authors.initials: I
authors.lastname: Basozabal
authors.affiliation: Department of Chemistry, University of Leicester, LE1 7RH, United Kingdom.
authors.forename: Antonio
authors.initials: A
authors.lastname: Guerreiro
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmacy, University of the Basque Country, 01006 Vitoria-Gasteiz, Spain.
authors.forename: Alberto
authors.initials: A
authors.lastname: Gomez-Caballero
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmacy, University of the Basque Country, 01006 Vitoria-Gasteiz, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Aranzazu Goicolea
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmacy, University of the Basque Country, 01006 Vitoria-Gasteiz, Spain. Electronic address: r.barrio@ehu.es.
authors.forename: Ramón J
authors.initials: RJ
authors.lastname: Barrio
mesh_terms: D003217:Conductometry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005504:Food Analysis / Q000295:instrumentation*; D005506:Food Contamination / Q000032:analysis*; D006632:Histamine / Q000032:analysis* / Q000737:chemistry; D054802:Molecular Imprinting / Q000295:instrumentation* / Q000379:methods; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D044367:Phase Transition
chemical_list: D006632:Histamine
keywords: Histamine; Molecularly imprinted polymers; Nanoparticles; Potentiometric sensor; Solid-phase imprinting