title: Antibiotic susceptibility and genotype patterns ofPseudomonas aeruginosafrom mechanical ventilation-associated pneumonia in intensive care units.
abstract: Pseudomonas aeruginosa(P. aeruginosa)is a leading cause of morbidity and mortality in patients with ventilation-associated pneumonia (VAP). It is difficult to treat this infection due to acquired resistance to various antibiotics. In order to understand the potential route of transmission, it is important to have detailed knowledge of the genotypes and antibiotic susceptibility ofP. aeruginosa. The aim of this study was to determine antibiotic susceptibility using the broth microdilution minimum inhibitory concentration (MIC) method and to apply the Randomly Amplified Polymorphic DNA (RAPD) typing method for VAP caused byP. aeruginosain 16 patients (8 men and 8 women; average age at inclusion, 67.8 years; range, 53-76 years). To determine antibiotic susceptibility, imipenem (IPM), cefepime (FEP) and meropenem (MEM) were administered for the treatment ofP. aeruginosa, yielding an effectiveness of 75, 62.5, and 62.5%, respectively. According to the National Committee for Clinical Laboratory Standards (NCCLS) breakpoints, 8 (50%) of the 16 mechanical ventilation (MV) isolates were resistant to ceftazidime (CAZ) and pipenacillin (PIP). Amikacin (AK) and aztreonam (AZT) were not as effective againstP. aeruginosa(75%). In addition,P. aeruginosawas completely resistant to ciprofloxacin (CIP). The MV isolates were susceptible to polymyxin B (PB). RAPD analysis revealed 12 genotypes for all the isolatedP. aeruginosa, separated into 4 patterns. The results demonstrated a high incidence ofP. aeruginosaisolated from VAP, with endogenous and cross infections being potential reasons forP. aeruginosaisolated from VAP in the intensive care units.
authors.affiliation: Department of Laboratory Medicine, Shanghai First People's Hospital, Medical College, Shanghai Jiao Tong University, Shanghai 200080, P.R. China.
authors.forename: Hui
authors.initials: H
authors.lastname: Xiao
authors.affiliation: Department of Respiratory Medicine, PuDong Hospital, Shanghai 200319, P.R. China.
authors.forename: Xiong
authors.initials: X
authors.lastname: Ye
authors.affiliation: Department of Laboratory Medicine, Shanghai First People's Hospital, Medical College, Shanghai Jiao Tong University, Shanghai 200080, P.R. China.
authors.forename: Qingzhong
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Department of Laboratory Medicine, Shanghai First People's Hospital, Medical College, Shanghai Jiao Tong University, Shanghai 200080, P.R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
keywords: Pseudomonas aeruginosa; antibiotic susceptibility; genotyping; ventilation associated pneumonia