title: Expression of dual-specificity phosphatase 5 pseudogene 1 (DUSP5P1) in tumor cells.
abstract: Sequencing of individual clones from a newly established cDNA library from the chemoresistant Hodgkin's lymphoma cell line L-1236 led to the isolation of a cDNA clone corresponding to a short sequence from chromosome 1. Reverse transcriptase-polymerase chain reaction indicated high expression of this sequence in Hodgkin's lymphoma derived cell lines but not in normal blood cells. Further characterization of this sequence and the surrounding genomic DNA revealed that this sequence is part of a human endogenous retrovirus locus. The sequence of this endogenous retrovirus is interrupted by a pseudogene of the dual specificity phosphatase 5 (DUSP5). Reverse transcriptase-polymerase chain reaction revealed high expression of this pseudogene (DUSP5P1) in HL cell lines but not in normal blood cells or Epstein-Barr virus-immortalized B cells. Cells from other tumor types (Burkitt's lymphoma, leukemia, neuroblastoma, Ewing sarcoma) also showed a higher DUSP5P1/DUSP5 ratio than normal cells. Furthermore, we observed that higher expression of DUSP5 in relation to DUSP5P1 correlated with the expression of the pro-apoptotic factor B cell leukemia/lymphoma 2-like 11 (BCL2L11) in peripheral blood cells and HL cells. Knock-down of DUSP5 in HL cells resulted in down-regulation of BCL2L11. Thus, the DUSP5/DUSP5P1 system could be responsible for regulation of BCL2L11 leading to inhibition of apoptosis in these tumor cells.
authors.affiliation: Department of Pediatrics, Martin Luther University Halle-Wittenberg, Halle, Germany.
authors.forename: Martin S
authors.initials: MS
authors.lastname: Staege
authors.affiliation: Department of Pediatrics, Martin Luther University Halle-Wittenberg, Halle, Germany.
authors.forename: Katja
authors.initials: K
authors.lastname: Müller
authors.affiliation: Department of Pediatrics, Martin Luther University Halle-Wittenberg, Halle, Germany.
authors.forename: Stefanie
authors.initials: S
authors.lastname: Kewitz
authors.affiliation: Department of Pediatrics, Martin Luther University Halle-Wittenberg, Halle, Germany.
authors.forename: Ines
authors.initials: I
authors.lastname: Volkmer
authors.affiliation: Department of Pediatrics, Martin Luther University Halle-Wittenberg, Halle, Germany.
authors.forename: Christine
authors.initials: C
authors.lastname: Mauz-Körholz
authors.affiliation: Department of Pediatrics, Martin Luther University Halle-Wittenberg, Halle, Germany.
authors.forename: Toralf
authors.initials: T
authors.lastname: Bernig
authors.affiliation: Department of Pediatrics, Martin Luther University Halle-Wittenberg, Halle, Germany.
authors.forename: Dieter
authors.initials: D
authors.lastname: Körholz
mesh_terms: D000595:Amino Acid Sequence; D017209:Apoptosis / Q000235:genetics; D051017:Apoptosis Regulatory Proteins / Q000235:genetics; D000072224:Bcl-2-Like Protein 11; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D002878:Chromosomes, Human, Pair 1 / Q000235:genetics; D054637:Dual-Specificity Phosphatases / Q000737:chemistry / Q000235:genetics*; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006689:Hodgkin Disease / Q000235:genetics / Q000473:pathology; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009369:Neoplasms / Q000235:genetics / Q000473:pathology; D020411:Oligonucleotide Array Sequence Analysis; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011518:Proto-Oncogene Proteins / Q000235:genetics; D011544:Pseudogenes / Q000235:genetics*; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid
chemical_list: D051017:Apoptosis Regulatory Proteins; C000606775:BCL2L11 protein, human; D000072224:Bcl-2-Like Protein 11; D008565:Membrane Proteins; D011518:Proto-Oncogene Proteins; C442320:DUSP5 protein, human; D054637:Dual-Specificity Phosphatases