title: Non-enzymatic DNA cleavage reaction induced by 5-ethynyluracil in methylamine aqueous solution and application to DNA concatenation.
abstract: DNA can be concatenated by hybridization of DNA fragments with protruding single-stranded termini. DNA cleavage occurring at a nucleotide containing a DNA base analogue is a useful method to obtain DNA with designed protruding termini. Here, we report a novel non-enzymatic DNA cleavage reaction for DNA concatenation. We found that DNA is cleaved at a nucleotide containing 5-ethynyluracil in a methylamine aqueous solution to generate 5'-phosphorylated DNA fragment as a cleavage product. We demonstrated that the reaction can be applied to DNA concatenation of PCR-amplified DNA fragments. This novel non-enzymatic DNA cleavage reaction is a simple practical approach for DNA concatenation.
authors.affiliation: Laboratory for Synthetic Biology, Quantitative Biology Center, RIKEN, Chuo-ku, Kobe, Japan.
authors.forename: Shuji
authors.initials: S
authors.lastname: Ikeda
authors.affiliation: Department of Systems Pharmacology, Graduate School of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.
authors.forename: Kazuki
authors.initials: K
authors.lastname: Tainaka
authors.affiliation: Laboratory for Synthetic Biology, Quantitative Biology Center, RIKEN, Chuo-ku, Kobe, Japan.
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Matsumoto
authors.affiliation: Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka, Japan.
authors.forename: Yuta
authors.initials: Y
authors.lastname: Shinohara
authors.affiliation: Department of Systems Pharmacology, Graduate School of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.
authors.forename: Koji L
authors.initials: KL
authors.lastname: Ode
authors.affiliation: Department of Systems Pharmacology, Graduate School of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.
authors.forename: Etsuo A
authors.initials: EA
authors.lastname: Susaki
authors.affiliation: Laboratory for Synthetic Biology, Quantitative Biology Center, RIKEN, Chuo-ku, Kobe, Japan; Laboratory for Systems Biology, Center for Developmental Biology, RIKEN, Chuo-ku, Kobe, Japan; Department of Systems Pharmacology, Graduate School of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo, Japan; Department of Biological Sciences, Graduate School of Science, Osaka University, Toyonaka, Osaka, Japan; Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka, Japan.
authors.forename: Hiroki R
authors.initials: HR
authors.lastname: Ueda
mesh_terms: D053837:DNA Cleavage; D044708:DNA, Concatenated / Q000737:chemistry*; D014498:Uracil / Q000031:analogs & derivatives* / Q000737:chemistry
chemical_list: D044708:DNA, Concatenated; C073482:eniluracil; D014498:Uracil