title: Evaluation of stability of live attenuated camelpox vaccine stabilized with different stabilizers and reconstituted with various diluents.
abstract: In this study, thermostability of a Vero cell attenuated live camelpox vaccine under conventional lyophilization conditions has been evaluated. Three stabilizers were used separately for freeze-drying the vaccine and the stability of the vaccine, both in freeze-dried and reconstituted forms at different temperatures was assessed. The study revealed that the camelpox vaccine lyophilized with TAA stabilizer found superior with a shelf life of 44 months, 227 days, 22 days and 20 days at 4, 25, 37 and 45 °C, respectively followed by LS stabilizer. In terms of half-life, TAA stabilizer proved better followed by LS and BUGS stabilizers at all temperatures except at 25 °C in which LS found relatively superior. Among the four diluents viz. 1x PBS (phosphate buffered saline, pH 7.4), 0.85% NaCl, distilled water and 1 M MgSO4, PBS was a better diluent followed by 0.85% NaCl. Both the diluents maintained the infectivity titer more than the minimum effective dose (3 log10TCID50 with a maximum titre of 6.53 log10TCID50 in both the diluents) for 60 h at 37 and 45 °C. However, 1 M MgSO4 found less suitable for camelpox vaccine dilution. The study indicates that the TAA and 1× PBS are the choice of stabilizer and diluent, respectively for camelpox vaccine.
authors.affiliation: Division of Virology, Indian Veterinary Research Institute, Mukteswar 263138, Nainital (Distt) Uttarakhand, India; Sheep Breeding Research Station, Tamil Nadu Veterinary and Animal Sciences University, Sandynallah 643 237, Tamil Nadu, India.
authors.forename: M
authors.initials: M
authors.lastname: Prabhu
authors.affiliation: Division of Virology, Indian Veterinary Research Institute, Mukteswar 263138, Nainital (Distt) Uttarakhand, India; FMD Laboratory, Indian Veterinary Research Institute, H A Farm, Hebbal, Bangalore 560 024, Karnataka, India. Electronic address: bhanu6467@gmail.com.
authors.forename: V
authors.initials: V
authors.lastname: Bhanuprakash
authors.affiliation: Division of Virology, Indian Veterinary Research Institute, Mukteswar 263138, Nainital (Distt) Uttarakhand, India.
authors.forename: G
authors.initials: G
authors.lastname: Venkatesan
authors.affiliation: Division of Virology, Indian Veterinary Research Institute, Mukteswar 263138, Nainital (Distt) Uttarakhand, India.
authors.forename: R
authors.initials: R
authors.lastname: Yogisharadhya
authors.affiliation: Division of Virology, Indian Veterinary Research Institute, Mukteswar 263138, Nainital (Distt) Uttarakhand, India; Department of Microbiology, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati 781 022, Assam, India.
authors.forename: D P
authors.initials: DP
authors.lastname: Bora
authors.affiliation: Division of Virology, Indian Veterinary Research Institute, Mukteswar 263138, Nainital (Distt) Uttarakhand, India; Project Directorate on Animal Disease Monitoring and Surveillance, H A Farm, Hebbal, Bangalore 560 024, Karnataka, India.
authors.forename: V
authors.initials: V
authors.lastname: Balamurugan
mesh_terms: D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D005612:Freeze Drying; D006207:Half-Life; D018155:Orthopoxvirus / Q000276:immunology*; D014709:Vero Cells; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D014765:Viral Vaccines
keywords: Camelpox virus; Thermostability; Vaccine