title: A method for the determination of total and reduced methimazole in various biological samples.
abstract: A simple, rapid, reproducible and sensitive method based on HPLC with ultraviolet detection was developed for the determination of methimazole (MMI) in animal tissues and plasma samples. Under the optimum experimental conditions, the calibration curves for MMI were linear in the tested range 0.5-20 mg kg(-1) tissue sample (mg l(-1) plasma) with correlation coefficients better than 0.99. The performance of the proposed method was tested for the determination of MMI levels in brain, liver, thyroid gland and plasma of MMI-treated hens, as well as in their eggs and embryos. The proposed method reduces time and simplifies the sample preparation procedure.
authors.affiliation: a Department of Environmental Chemistry, Faculty of Chemistry , University of Lodz , Lodz , Poland.
authors.forename: Grażyna
authors.initials: G
authors.lastname: Chwatko
authors.forename: Veerle M
authors.initials: VM
authors.lastname: Darras
authors.forename: Edward
authors.initials: E
authors.lastname: Bald
mesh_terms: D000818:Animals; D013956:Antithyroid Agents / Q000032:analysis* / Q000097:blood / Q000633:toxicity; D001923:Brain Chemistry; D002642:Chick Embryo; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004350:Drug Residues / Q000032:analysis* / Q000633:toxicity; D004531:Eggs / Q000032:analysis / Q000633:toxicity; D005506:Food Contamination / Q000032:analysis*; D006801:Humans; D008099:Liver / Q000737:chemistry; D008713:Methimazole / Q000032:analysis* / Q000097:blood / Q000633:toxicity; D010084:Oxidation-Reduction; D015203:Reproducibility of Results; D013961:Thyroid Gland / Q000737:chemistry
chemical_list: D013956:Antithyroid Agents; D008713:Methimazole
keywords: chicken tissue; methimazole; method development; tapazole; thiamazole; tissue homogenate