title: A case-control study evaluating RT-PCR/ESI-MS technology compared to direct fluorescent antibody and xTAG RVP PCR.
abstract: Waste nasopharyngeal swabs (N = 244) were evaluated by the reverse-transcriptase polymerase chain reaction/electrospray ionization mass spectrometry PLEX-ID Broad Respiratory Virus Surveillance Kit version 2.5 compared to direct fluorescent antibody and xTAG Respiratory Virus Panel for percent agreement, sensitivity, and specificity. Sensitivity and specificity were 91% (111/122) and 95.1% (116/122), respectively. Sensitivity by virus, except parainfluenza, was 92.9-100%, and specificity was 99-100%.
authors.affiliation: Johns Hopkins University School of Medicine, Division of Infectious Diseases, Baltimore, MD, USA. Electronic address: Jhardic1@jhmi.edu.
authors.forename: Justin
authors.initials: J
authors.lastname: Hardick
authors.affiliation: Johns Hopkins University, Department of Emergency Medicine, Baltimore, MD, USA.
authors.forename: Sufyan
authors.initials: S
authors.lastname: Sadiq
authors.affiliation: Johns Hopkins University, Department of Emergency Medicine, Baltimore, MD, USA.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Perelstein
authors.affiliation: Johns Hopkins University, Department of Emergency Medicine, Baltimore, MD, USA.
authors.forename: Stephen
authors.initials: S
authors.lastname: Peterson
authors.affiliation: Johns Hopkins University School of Medicine, Division of Infectious Diseases, Baltimore, MD, USA; Johns Hopkins University, Department of Emergency Medicine, Baltimore, MD, USA.
authors.forename: Richard
authors.initials: R
authors.lastname: Rothman
authors.affiliation: Johns Hopkins University School of Medicine, Division of Infectious Diseases, Baltimore, MD, USA; Johns Hopkins University, Department of Emergency Medicine, Baltimore, MD, USA.
authors.forename: Charlotte A
authors.initials: CA
authors.lastname: Gaydos
mesh_terms: D016022:Case-Control Studies; D019085:Fluorescent Antibody Technique, Direct / Q000379:methods; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012141:Respiratory Tract Infections / Q000175:diagnosis* / Q000821:virology; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D014777:Virus Diseases / Q000175:diagnosis* / Q000821:virology
keywords: DFA; RT-PCR/ESI-MS; Respiratory virus diagnostics; xTAG RVP