title: Anti-diabetic effects of CTB-APSL fusion protein in type 2 diabetic mice.
abstract: To determine whether cholera toxin B subunit and active peptide from shark liver (CTB-APSL) fusion protein plays a role in treatment of type 2 diabetic mice, the CTB-APSL gene was cloned and expressed in silkworm (Bombyx mori) baculovirus expression vector system (BEVS), then the fusion protein was orally administrated at a dose of 100 mg/kg for five weeks in diabetic mice. The results demonstrated that the oral administration of CTB-APSL fusion protein can effectively reduce the levels of both fasting blood glucose (FBG) and glycosylated hemoglobin (GHb), promote insulin secretion and improve insulin resistance, significantly improve lipid metabolism, reduce triglycerides (TG), total cholesterol (TC) and low density lipoprotein (LDL) levels and increase high density lipoprotein (HDL) levels, as well as effectively improve the inflammatory response of type 2 diabetic mice through the reduction of the levels of inflammatory cytokines tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6). Histopathology shows that the fusion protein can significantly repair damaged pancreatic tissue in type 2 diabetic mice, significantly improve hepatic steatosis and hepatic cell cloudy swelling, reduce the content of lipid droplets in type 2 diabetic mice, effectively inhibit renal interstitial inflammatory cells invasion and improve renal tubular epithelial cell nucleus pyknosis, thus providing an experimental basis for the development of a new type of oral therapy for type 2 diabetes.
authors.affiliation: College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China. liuyunlong5566@163.com.
authors.forename: Yunlong
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China. lion8907@163.com.
authors.forename: Zhangzhao
authors.initials: Z
authors.lastname: Gao
authors.affiliation: College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China. guoqingtuo@163.com.
authors.forename: Qingtuo
authors.initials: Q
authors.lastname: Guo
authors.affiliation: College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China. wangtao900121@163.com.
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.affiliation: College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China. luconger0302@163.com.
authors.forename: Conger
authors.initials: C
authors.lastname: Lu
authors.affiliation: College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China. carolynchency@163.com.
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.affiliation: College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China. csheng@zstu.edu.cn.
authors.forename: Qing
authors.initials: Q
authors.lastname: Sheng
authors.affiliation: College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China. chj1999@126.com.
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
authors.affiliation: College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China. wuxinzm@zstu.edu.cn.
authors.forename: Zuoming
authors.initials: Z
authors.lastname: Nie
authors.affiliation: College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China. yaozhou@zstu.edu.cn.
authors.forename: Yaozhou
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China. wuwutong@gmail.com.
authors.forename: Wutong
authors.initials: W
authors.lastname: Wu
authors.affiliation: College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China. zhengbingl@zstu.edu.cn.
authors.forename: Zhengbing
authors.initials: Z
authors.lastname: Lv
authors.affiliation: College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China. shujianhong@zstu.edu.cn.
authors.forename: Jianhong
authors.initials: J
authors.lastname: Shu
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics / Q000378:metabolism; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight; D012831:Bombyx / Q000821:virology; D002772:Cholera Toxin / Q000737:chemistry / Q000494:pharmacology*; D004279:DNA, Viral / Q000235:genetics; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy; D003924:Diabetes Mellitus, Type 2 / Q000188:drug therapy*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005677:G(M1) Ganglioside / Q000378:metabolism; D005822:Genetic Vectors; D006442:Glycated Hemoglobin / Q000032:analysis; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D000960:Hypolipidemic Agents / Q000494:pharmacology; D007328:Insulin / Q000378:metabolism; D007333:Insulin Resistance; D007668:Kidney / Q000473:pathology; D008055:Lipids / Q000097:blood; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D012754:Sharks; D013154:Spleen / Q000473:pathology; D014760:Viral Fusion Proteins / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D001786:Blood Glucose; D002772:Cholera Toxin; D004279:DNA, Viral; D005677:G(M1) Ganglioside; D006442:Glycated Hemoglobin; D007004:Hypoglycemic Agents; D000960:Hypolipidemic Agents; D007328:Insulin; D008055:Lipids; D014760:Viral Fusion Proteins