title: Microencapsulation of purified amylase enzyme from pitaya (Hylocereus polyrhizus) peel in Arabic gum-chitosan using freeze drying.
abstract: Amylase is one of the most important enzymes in the world due to its wide application in various industries and biotechnological processes. In this study, amylase enzyme from Hylocereus polyrhizus was encapsulated for the first time in an Arabic gum-chitosan matrix using freeze drying. The encapsulated amylase retained complete biocatalytic activity and exhibited a shift in the optimum temperature and considerable increase in the pH and temperature stabilities compared to the free enzyme. Encapsulation of the enzyme protected the activity in the presence of ionic and non-ionic surfactants and oxidizing agents (H₂O₂) and enhanced the shelf life. The storage stability of amylase is found to markedly increase after immobilization and the freeze dried amylase exhibited maximum encapsulation efficiency value (96.2%) after the encapsulation process. Therefore, the present study demonstrated that the encapsulation of the enzyme in a coating agent using freeze drying is an efficient method to keep the enzyme active and stable until required in industry.
authors.affiliation: Department of Food Technology, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor 43400, Malaysia. mehrnoush_amid@yahoo.com.
authors.forename: Mehrnoush
authors.initials: M
authors.lastname: Amid
authors.affiliation: Department of Food Technology, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor 43400, Malaysia. myazid@upm.edu.my.
authors.forename: Yazid
authors.initials: Y
authors.lastname: Manap
authors.affiliation: Department of Food Technology, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor 43400, Malaysia. norkhanani@gmail.com.
authors.forename: Nor Khanani
authors.initials: NK
authors.lastname: Zohdi
mesh_terms: D000681:Amylases / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism / Q000648:ultrastructure; D029421:Cactaceae / Q000737:chemistry* / Q000201:enzymology; D048271:Chitosan / Q000737:chemistry*; D004789:Enzyme Activation / Q000187:drug effects; D004795:Enzyme Stability / Q000187:drug effects; D005612:Freeze Drying / Q000379:methods; D006170:Gum Arabic / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D016877:Oxidants / Q000494:pharmacology; D010316:Particle Size; D013501:Surface-Active Agents / Q000494:pharmacology; D013696:Temperature; D013816:Thermodynamics
chemical_list: D016877:Oxidants; D013501:Surface-Active Agents; D006170:Gum Arabic; D048271:Chitosan; D000681:Amylases