title: Study of metalloproteins using continuous wave electron paramagnetic resonance (EPR).
abstract: Electron paramagnetic resonance (EPR) is an invaluable tool when studying systems with paramagnetic centers. It is a sensitive spectroscopic method, which can be used with dilute samples in aqueous buffer solutions. Here, we describe the basic procedure for recording an X-band EPR spectrum of a metalloprotein sample at low temperature. We also discuss basic optimization techniques to provide spectra with a high signal to noise ratio and minimum distortion.
authors.affiliation: Laboratoire de Chimie Inorganique et Biologique, Institut de Nanosciences et Cryogénie, CEA-Grenoble, 17 avenue des Martyrs, 38054, Grenoble, France, serge.gambarelli@cea.fr.
authors.forename: Serge
authors.initials: S
authors.lastname: Gambarelli
authors.forename: Vincent
authors.initials: V
authors.lastname: Maurel
mesh_terms: D004578:Electron Spin Resonance Spectroscopy / Q000379:methods*; D008667:Metalloproteins / Q000032:analysis*; D012815:Signal Processing, Computer-Assisted; D013696:Temperature
chemical_list: D008667:Metalloproteins