title: α-Synuclein is prone to interaction with the GC-box-like sequence in vitro.
abstract: α-Synuclein (α-syn) is a presynaptic protein that is widely implicated in the pathophysiology of Parkinson's disease, a neurodegenerative disorder characterized by a progressive loss of dopaminergic neurons and the formation of Lewy bodies. Evidence suggests that α-syn could be imported into the nucleus and subsequently disrupt normal neuronal function. The existence of α-syn in the nucleus provides the possibility of interaction with DNA leading to gene transcript regulation. Thus, CD spectra were used to determine the specific DNA sequence with which α-syn is most likely to interact. Our results indicated that α-syn was prone to preferentially interact with the GC-box-like sequence in vitro at a ratio of 2:1 or less (α-syn: the GC-box-like sequence).
authors.forename: Kai-Li
authors.initials: KL
authors.lastname: Ma
authors.forename: Lian-Kun
authors.initials: LK
authors.lastname: Song
authors.forename: Yu-He
authors.initials: YH
authors.lastname: Yuan
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jin-Ling
authors.initials: JL
authors.lastname: Yang
authors.forename: Ping
authors.initials: P
authors.lastname: Zhu
authors.forename: Nai-Hong
authors.initials: NH
authors.lastname: Chen
mesh_terms: D004247:DNA / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism; D015870:Gene Expression / Q000502:physiology*; D005786:Gene Expression Regulation / Q000502:physiology*; D006801:Humans; D016631:Lewy Bodies / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D010843:Pichia; D051844:alpha-Synuclein / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; D009419:Nerve Tissue Proteins; D051844:alpha-Synuclein; D004247:DNA