title: Determination of lysosomal exoglycosidases in human saliva.
abstract: BACKGROUND
  Currently we observe a growing interest in human saliva as a non-invasive material for diagnosis and monitoring of general and oral diseases.
  
  
  METHODS
  The aim of our study was adaptation of the Marciniak et al. (Marciniak J, Zalewska A, Popko J, Zwierz K, 2006, Clin Chem Lab Med 44: 933-937) method for determination of HEX and GLU activity in synovial fluid, and for determination of: HEX and GLU, as well as MAN, GAL, and FUC activity in human saliva.
  
  
  RESULTS
  Under optimal conditions, 10 μl of saliva for HEX, and 30 μl for GLU, MAN, GAL and FUC, were sufficient for determination of human salivary exoglycosidases activity with variation coefficient ranging from 0.89 for GLU to 0.99 for GAL.
  
  
  CONCLUSIONS
  The adapted method for exoglycosidases activity determination in human saliva is sufficiently sensitive and precise to use in clinical diagnosis.
authors.affiliation: Medical Institute, College of Computer Science and Business Administration, Lomza, Poland.
authors.forename: Sylwia
authors.initials: S
authors.lastname: Chojnowska
authors.affiliation: Department of Conservative Dentistry, Medical University in Bialystok, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Zalewska
authors.affiliation: The Institute of Health Care, The Higher Vocational School, Suwalki, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Knaś
authors.affiliation: Department of Psychiatry, Medical University in Bialystok, Poland.
authors.forename: Napoleon
authors.initials: N
authors.lastname: Waszkiewicz
authors.affiliation: Department of Conservative Dentistry, Medical University in Bialystok, Poland.
authors.forename: Danuta
authors.initials: D
authors.lastname: Waszkiel
authors.affiliation: Medical College of the Universal Education Society, Lomza, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Kossakowska
authors.affiliation: Medical College of the Universal Education Society, Lomza, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Zwierz
mesh_terms: D000328:Adult; D001172:Arthritis, Rheumatoid / Q000473:pathology; D005260:Female; D056726:Genetic Association Studies; D006801:Humans; D008247:Lysosomes / Q000201:enzymology; D010510:Periodontal Diseases / Q000175:diagnosis* / Q000201:enzymology / Q000473:pathology; D012463:Saliva / Q000201:enzymology; D005644:alpha-L-Fucosidase / Q000096:biosynthesis; D043323:alpha-Mannosidase / Q000096:biosynthesis; D001616:beta-Galactosidase / Q000096:biosynthesis; D001619:beta-N-Acetylhexosaminidases / Q000096:biosynthesis
chemical_list: D001616:beta-Galactosidase; D043323:alpha-Mannosidase; D005644:alpha-L-Fucosidase; D001619:beta-N-Acetylhexosaminidases