title: Mitochondrial proteomic analysis of ecdysterone protection against oxidative damage in human lens epithelial cells.
abstract: OBJECTIVE
  To investigate the protective effects of the natural medicinal monomer ecdysterone (ECR) with estrogenic activity against oxidative damage in human lens epithelial cells B3 (HLE-B3) caused by hydrogen peroxide 21(H2O2) and to pursue the possible mitochondrial proteomic regularity of the protective effects.
  
  
  METHODS
  HLE-B3 cells were treated with H2O2 (300µmol/L), β-estuarial (E2; 10(-8)mol/L) and H2O2, ECR (10(-6)mol/L) and H2O2, or left untreated. Altered expression of all mitochondrial proteins was analyzed by protein array and surface-enhanced laser desorption ionization time of flight mass spectrometry (SELDI-TOF-MS). The mass/charge (M/Z) ratios of each peak were tested by the Kruskal-Wallis rank sum test, and the protein peak value of the M/Z ratio for each treatment by pair comparison was analyzed with the Nemenyi test.
  
  
  RESULTS
  H2O2 up-regulated expression of two protein spots (with M/Z of 6 532 and 6 809). When E2 mitigated the oxidative damage, the expression of one protein spot (M/Z 6 532) was down-regulated. In contrast, ECR down-regulated both of protein spots (M/Z 6 532 and 6 809).
  
  
  CONCLUSIONS
  ECR could effectively inhibite H2O2 induced oxidative damage in HLE-B3 cells. The protein spot at M/Z of 6 532 might be the target spot of ECR against oxidative damage induced by H2O2.
authors.affiliation: Department of Ophthalmology, Second Affiliated Peoples Hospital of Fujian University of Traditional Chinese Medicine, Fuzhou 350003, Fujian Province, China.
authors.forename: Chun-Yan
authors.initials: CY
authors.lastname: Feng
authors.affiliation: Research Center of Pathophysiology, Fujian University of Traditional Chinese Medicine, Fuzhou 350003, Fujian Province, China.
authors.forename: Xiu-Rong
authors.initials: XR
authors.lastname: Huang
authors.affiliation: Department of Ophthalmology, Second Affiliated Peoples Hospital of Fujian University of Traditional Chinese Medicine, Fuzhou 350003, Fujian Province, China.
authors.forename: Ming-Xin
authors.initials: MX
authors.lastname: Qi
authors.affiliation: Research Center of Pathophysiology, Fujian University of Traditional Chinese Medicine, Fuzhou 350003, Fujian Province, China.
authors.forename: Song-Wen
authors.initials: SW
authors.lastname: Tang
authors.affiliation: Department of Ophthalmology, Second Affiliated Peoples Hospital of Fujian University of Traditional Chinese Medicine, Fuzhou 350003, Fujian Province, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Chen
authors.affiliation: Department of Ophthalmology, Second Affiliated Peoples Hospital of Fujian University of Traditional Chinese Medicine, Fuzhou 350003, Fujian Province, China.
authors.forename: Yan-Hong
authors.initials: YH
authors.lastname: Hu
authors.affiliation: Department of Ophthalmology, Second Affiliated Peoples Hospital of Fujian University of Traditional Chinese Medicine, Fuzhou 350003, Fujian Province, China.
authors.forename: Fa-Jie
authors.initials: FJ
authors.lastname: Ke
authors.affiliation: Department of Laboratory, Fujian University of Traditional Chinese Medicine subsidiary Rehabilitation Hospital, Fuzhou 350003, Fujian Province, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
keywords: ecdysterone; lens epithelial cell; mitochondrial proteomics; senile cataract