title: Description of a new Mycobacterium intracellulare pattern of PCR restriction enzyme analysis of hsp65 gene.
abstract: This work comprises 9 pulmonary nontuberculous mycobateria isolates obtained from sputum of 4 different patients from Brazil. The sequencing and phylogenetic analysis allowed their accurate identification as Mycobacterium intracellulare. We report a mutation at position 453 creating a new HaeIII cutting site and, therefore, a new PRA-hsp65 M. intracellulare profile.
authors.affiliation: National Reference Laboratory for Tuberculosis, Centro de Referência Professor Hélio Fraga, Escola Nacional de Saúde Pública, Fiocruz, RJ, Brazil.
authors.forename: Paulo Cesar de Souza
authors.initials: PC
authors.lastname: Caldas
authors.affiliation: National Reference Laboratory for Tuberculosis, Centro de Referência Professor Hélio Fraga, Escola Nacional de Saúde Pública, Fiocruz, RJ, Brazil.
authors.forename: Carlos Eduardo Dias
authors.initials: CE
authors.lastname: Campos
authors.affiliation: National Reference Laboratory for Tuberculosis, Centro de Referência Professor Hélio Fraga, Escola Nacional de Saúde Pública, Fiocruz, RJ, Brazil.
authors.forename: Lusiano Motta
authors.initials: LM
authors.lastname: Dos Reis
authors.affiliation: National Reference Laboratory for Tuberculosis, Centro de Referência Professor Hélio Fraga, Escola Nacional de Saúde Pública, Fiocruz, RJ, Brazil.
authors.forename: Nicole Victor
authors.initials: NV
authors.lastname: Ferreira
authors.affiliation: National Reference Laboratory for Tuberculosis, Centro de Referência Professor Hélio Fraga, Escola Nacional de Saúde Pública, Fiocruz, RJ, Brazil.
authors.forename: Luciana Distásio
authors.initials: LD
authors.lastname: de Carvalho
authors.affiliation: National Reference Laboratory for Tuberculosis, Centro de Referência Professor Hélio Fraga, Escola Nacional de Saúde Pública, Fiocruz, RJ, Brazil.
authors.forename: Mariza Villas Boas
authors.initials: MV
authors.lastname: da Silva
authors.affiliation: National Reference Laboratory for Tuberculosis, Centro de Referência Professor Hélio Fraga, Escola Nacional de Saúde Pública, Fiocruz, RJ, Brazil.
authors.forename: Reginalda Ferreira de Melo
authors.initials: RF
authors.lastname: Medeiros
authors.affiliation: National Reference Laboratory for Tuberculosis, Centro de Referência Professor Hélio Fraga, Escola Nacional de Saúde Pública, Fiocruz, RJ, Brazil.
authors.forename: Fátima Cristina Onofre Fandinho
authors.initials: FC
authors.lastname: Montes
authors.affiliation: National Reference Laboratory for Tuberculosis, Centro de Referência Professor Hélio Fraga, Escola Nacional de Saúde Pública, Fiocruz, RJ, Brazil. Electronic address: jepramos@ensp.fiocruz.br.
authors.forename: Jesus Pais
authors.initials: JP
authors.lastname: Ramos
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D001938:Brazil; D018834:Chaperonin 60 / Q000235:genetics*; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D058889:Molecular Typing; D015269:Mycobacterium avium Complex / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D015270:Mycobacterium avium-intracellulare Infection / Q000382:microbiology; D010802:Phylogeny; D011014:Pneumonia / Q000382:microbiology; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D013183:Sputum / Q000382:microbiology
chemical_list: D001426:Bacterial Proteins; D018834:Chaperonin 60; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; C074119:heat-shock protein 65, Mycobacterium
keywords: Mycobacterium intracellulare; Nontuberculous mycobacteria; PRA-hsp65