title: The administration of milk fermented by the probiotic Lactobacillus casei CRL 431 exerts an immunomodulatory effect against a breast tumour in a mouse model.
abstract: Antitumour activity is one of the health-promoting effects attributed to probiotics specially analysed from preclinical models, mostly murine. Here, the effect of milk fermented by the probiotic bacterium Lactobacillus casei CRL 431, on a murine breast cancer model was analysed. Mice were fed with milk fermented by Lactobacillus casei or unfermented milk before and after tumour injection. Rate of tumour development, cytokines in serum, IgA, CD4, CD8, F4/80 and cytokines positive cells in mammary glands were determined. Microvasculature in the tumour tissues was monitored. The effect of fermented milk administration after tumour injection was also evaluated. It was observed that probiotic administration delayed or blocked tumour development. This effect was associated to modulation of the immune response triggered by the tumour. The area occupied by blood vessels decreased in the tumours from mice given fermented milk which agrees with their small tumours, and fewer side effects. Finally, it was observed that probiotic administration after tumour detection was also beneficial to delay the tumour growth. In conclusion, we showed in this study the potential of milk fermented by the probiotic Lactobacillus casei CRL431 to stimulate the immune response against this breast tumour, avoiding or delaying its growth when it was preventively administrated and also when the administration started after tumour cells injection.
authors.affiliation: Centro de Referencia para Lactobacilos (CERELA-CONICET), Chacabuco 145, San Miguel de Tucumán, T4000ILC Tucumán, Argentina.
authors.forename: Félix
authors.initials: F
authors.lastname: Aragón
authors.affiliation: Laboratorio de Anatomía patológica, Facultad de Odontología, Universidad Nacional de Tucumán, San Miguel de Tucumán, Tucumán, Argentina.
authors.forename: Silvia
authors.initials: S
authors.lastname: Carino
authors.affiliation: Centro de Referencia para Lactobacilos (CERELA-CONICET), Chacabuco 145, San Miguel de Tucumán, T4000ILC Tucumán, Argentina; Cátedra de Inmunología, Facultad de Bioquimíca, Química y Farmacia, Universidad Nacional de Tucumán, San Miguel de Tucumán, Tucumán, Argentina.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Perdigón
authors.affiliation: Centro de Referencia para Lactobacilos (CERELA-CONICET), Chacabuco 145, San Miguel de Tucumán, T4000ILC Tucumán, Argentina. Electronic address: demoreno@cerela.org.ar.
authors.forename: Alejandra
authors.initials: A
authors.lastname: de Moreno de LeBlanc
mesh_terms: D000818:Animals; D000943:Antigens, Differentiation / Q000097:blood; D015704:CD4 Antigens / Q000097:blood; D016827:CD8 Antigens / Q000097:blood; D049109:Cell Proliferation; D004195:Disease Models, Animal; D005260:Female; D005285:Fermentation; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood; D016753:Interleukin-10 / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D007780:Lacticaseibacillus casei / Q000276:immunology / Q000378:metabolism*; D008321:Mammary Glands, Animal / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D042361:Mammary Glands, Human / Q000473:pathology; D015674:Mammary Neoplasms, Animal / Q000188:drug therapy / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008892:Milk / Q000382:microbiology*; D019936:Probiotics / Q000627:therapeutic use
chemical_list: D000943:Antigens, Differentiation; D015704:CD4 Antigens; D016827:CD8 Antigens; C556062:IL10 protein, mouse; D007070:Immunoglobulin A; D015850:Interleukin-6; C036763:monocyte-macrophage differentiation antigen; D016753:Interleukin-10
keywords: Angiogenesis; Breast cancer; Immune response; Mouse model; Probiotic