title: An electroactive biotin-doped polypyrrole substrate that immobilizes and releases EpCAM-positive cancer cells.
abstract: The specific capture and remotely controlled release of the EpCAM-positive cancer cells from biotin-doped polypyrrole (Ppy) films in response to an electrical potential is presented. As Ppy allows the direct incorporation of biotin molecules during the electrochemical process, densely packed biotin molecules can serve as the binding sites for streptavidin-tagged biomolecular complexes. This study demonstrates not only the enhanced capture and enrichment of EpCAM-positive cancer cells but also "on-demand" release of the viable cells from conductive Ppy in an electrical-potential-dependent way. This novel approach is of great importance in a diverse range of applications, and in particular in cancer diagnostics and screening.
authors.affiliation: New Experimental Therapeutic Branch, National Cancer Center, 111 Jungbalsan-ro, Ilsandong-gu, Goyang, Gyeonggi-do 410-769 (South Korea).
authors.forename: Seunghyun
authors.initials: S
authors.lastname: Jeon
authors.forename: Jeong-Mi
authors.initials: JM
authors.lastname: Moon
authors.forename: Eun Sook
authors.initials: ES
authors.lastname: Lee
authors.forename: Yon Hui
authors.initials: YH
authors.lastname: Kim
authors.forename: Youngnam
authors.initials: Y
authors.lastname: Cho
mesh_terms: D000951:Antigens, Neoplasm / Q000737:chemistry* / Q000378:metabolism; D001710:Biotin / Q000737:chemistry* / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000737:chemistry* / Q000378:metabolism; D004553:Electric Conductivity; D000071858:Epithelial Cell Adhesion Molecule; D006801:Humans; D009369:Neoplasms / Q000378:metabolism / Q000473:pathology*; D011108:Polymers / Q000737:chemistry* / Q000378:metabolism; D011758:Pyrroles / Q000737:chemistry* / Q000378:metabolism; D019809:Streptavidin / Q000737:chemistry / Q000378:metabolism; D013499:Surface Properties; D014407:Tumor Cells, Cultured
chemical_list: D000951:Antigens, Neoplasm; D015815:Cell Adhesion Molecules; C499379:EPCAM protein, human; D000071858:Epithelial Cell Adhesion Molecule; D011108:Polymers; D011758:Pyrroles; C067635:polypyrrole; D001710:Biotin; D019809:Streptavidin
keywords: EpCAM; HeLa cells; MCF7 cells; conducting polymers; electrical stimulation