title: Isolation, characterization and antihyperlipidemic activity of secoisolariciresinol diglucoside in poloxamer-407-induced experimental hyperlipidemia.
abstract: BACKGROUND
  Linum usitatissimum L. (Linaceae), commonly known as flaxseed, is a good source of dietary fiber and lignans. Earlier we reported cardioprotective, antihyperlipidemic, and in vitro antioxidant activity of flax lignan concentrate (FLC) obtained from flaxseed.
  
  
  OBJECTIVE
  To isolate secoisolariciresinol diglucoside (SDG) from FLC and to evaluate the antihyperlipidemic activity of SDG in poloxamer-407 (P-407)-induced hyperlipidaemic mice.
  
  
  METHODS
  FLC was subjected to column chromatography and further subjected to preparative HPTLC to isolate SDG. The chemical structure of the isolated compound was elucidated by UV, IR, (1)H NMR, (13)C NMR, DEPT, COSY, HSQC, HMBC, ROESY, MS, and specific optical rotation was recorded. Further, we have investigated the antihyperlipidaemic effect of SDG (20 mg/kg) in P-407-induced hyperlipidaemic rats. Hyperlipidaemia was induced by intraperitoneal administration of P-407 (30% w/v). Serum lipid parameters such as total cholesterol (TC), triglycerides (TG), and high-density lipoprotein cholesterol (HDL-C) levels were measured.
  
  
  CONCLUSIONS
  The structure and stereochemistry of the isolated compound were confirmed on the basis of 1D and 2D spectral data and characterized as SDG. Finally, isolated pure SDG was screened using a P-407-induced mice model for its antihyperlipidemic action using serum lipid parameters. The isolated SDG (20 mg/kg) significantly reduced serum cholesterol, triglyceride (p < 0.001), very low-density lipoprotein (p < 0.05), and non-significantly increased HDL-C.
  
  
  CONCLUSIONS
  Finally, it was concluded unequivocally that SDG showed antihyperlipidaemic effects in P-407-induced hyperlipidaemic mice. Isolated pure SDG confirms that SDG is beneficial in the prevention of experimental hyperlipidemia in laboratory animals.
authors.affiliation: D epartment of Pharmacology, Poona College of Pharmacy, Bharati Vidyapeeth Deemed University , Pune, Maharashtra , India .
authors.forename: Anand A
authors.initials: AA
authors.lastname: Zanwar
authors.forename: Mahabaleshwar V
authors.initials: MV
authors.lastname: Hegde
authors.forename: Supada R
authors.initials: SR
authors.lastname: Rojatkar
authors.forename: Kiran B
authors.initials: KB
authors.lastname: Sonawane
authors.forename: P R
authors.initials: PR
authors.lastname: Rajamohanan
authors.forename: Subhash L
authors.initials: SL
authors.lastname: Bodhankar
mesh_terms: D000818:Animals; D002072:Butylene Glycols / Q000302:isolation & purification / Q000494:pharmacology*; D002855:Chromatography, Thin Layer / Q000379:methods; D004195:Disease Models, Animal; D019597:Flax / Q000737:chemistry*; D005960:Glucosides / Q000302:isolation & purification / Q000494:pharmacology*; D006949:Hyperlipidemias / Q000517:prevention & control*; D000960:Hypolipidemic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D008055:Lipids / Q000097:blood; D051379:Mice; D020442:Poloxamer / Q000633:toxicity; D051381:Rats; D013057:Spectrum Analysis / Q000379:methods
chemical_list: D002072:Butylene Glycols; D005960:Glucosides; D000960:Hypolipidemic Agents; D008055:Lipids; D020442:Poloxamer; C090142:secoisolariciresinol diglucoside
keywords: Column chromatography; Linum usitatissimum; flaxseed