title: In vitro reconstitution of dynamic microtubules interacting with actin filament networks.
abstract: Interactions between microtubules and actin filaments (F-actin) are essential for eukaryotic cell migration, polarization, growth, and division. Although the importance of these interactions has been long recognized, the inherent complexity of the cell interior hampers a detailed mechanistic study of how these two cytoskeletal systems influence each other. In this chapter, we show how in vitro reconstitution can be employed to study how actin filaments and dynamic microtubules affect each other's organization. While we focus here on the effect of steric interactions, these assays provide an ideal starting point to develop more complex studies through the addition of known F-actin-microtubule cross-linkers, or myosin II motors.
authors.affiliation: FOM Institute AMOLF, Science Park, Amsterdam, The Netherlands.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Preciado López
authors.affiliation: FOM Institute AMOLF, Science Park, Amsterdam, The Netherlands.
authors.forename: Florian
authors.initials: F
authors.lastname: Huber
authors.affiliation: Division of Cell Biology, Faculty of Science, Utrecht University, Padualaan, Utrecht, The Netherlands.
authors.forename: Ilya
authors.initials: I
authors.lastname: Grigoriev
authors.affiliation: Laboratory of Biomolecular Research, Paul Scherrer Institut, Villigen, Switzerland.
authors.forename: Michel O
authors.initials: MO
authors.lastname: Steinmetz
authors.affiliation: Division of Cell Biology, Faculty of Science, Utrecht University, Padualaan, Utrecht, The Netherlands.
authors.forename: Anna
authors.initials: A
authors.lastname: Akhmanova
authors.affiliation: FOM Institute AMOLF, Science Park, Amsterdam, The Netherlands. Electronic address: dogterom@amolf.nl.
authors.forename: Marileen
authors.initials: M
authors.lastname: Dogterom
authors.affiliation: FOM Institute AMOLF, Science Park, Amsterdam, The Netherlands. Electronic address: g.koenderink@amolf.nl.
authors.forename: Gijsje H
authors.initials: GH
authors.lastname: Koenderink
mesh_terms: D016475:3T3 Cells; D008841:Actin Cytoskeleton / Q000378:metabolism* / Q000648:ultrastructure*; D000199:Actins / Q000378:metabolism / Q000648:ultrastructure; D000818:Animals; D003599:Cytoskeleton / Q000378:metabolism / Q000648:ultrastructure; D051379:Mice; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods; D008870:Microtubules / Q000378:metabolism* / Q000648:ultrastructure*; D011817:Rabbits
chemical_list: D000199:Actins
keywords: +TIPs; Actin; Cytoskeletal organization; In vitro; Microtubules