title: Disulfide bonds regulate binding of exogenous ligand to human cytoglobin.
abstract: Cytoglobin (Cgb) was discovered a decade ago and is a fourth member of the group of hexacoordinated globin-folded proteins. Although some crystal structures have been reported and several functions have been proposed for Cgb, its physiological role remains uncertain. In this study, we measured cyanide binding to the ferric state of the wild-type (WT) Cgb, and found that the binding consisted of multiple steps. These results indicated that Cgb may be comprised of several forms, and the presence of monomers, dimers, and tetramers was subsequently confirmed by SDS-PAGE. Remarkably, each species contained two distinguishable forms, and, in the monomer, analyses of alternative cysteine states suggested the presence of an intramolecular disulfide bond (monomer SS form) and a structure with unpaired thiol groups (monomer SH form). These confirmed that forms were separated by gel-exclusion chromatography, and that the cyanide binding of the separated fractions was again measured; they showed different affinities for cyanide, with the monomer fraction showing the highest affinity. In addition, the ferrous state in each fraction showed distinct carbon monoxide (CO)-binding properties, and the affinities for cyanide and CO suggested a linear correlation. Furthermore, we also prepared several variants involving the two cysteine residues. The C38S and C83S variants showed a binding affinity for cyanide similar to the value for the monomer SH form, and hence the fraction with the highest affinity for exogenous ligands was designated as a monomer SS form. We concluded that polymerization could be a mechanism that triggers the exertion of various physiological functions of this protein and that an appropriate disulfide bond between the two cysteine residues was critical for regulating the binding affinity of Cgb, which can act as a ROS scavenger, for exogenous ligands.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Osaka University, Osaka, Japan.
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Tsujino
authors.affiliation: Graduate School of Pharmaceutical Sciences, Osaka University, Osaka, Japan. Electronic address: taku@phs.osaka-u.ac.jp.
authors.forename: Taku
authors.initials: T
authors.lastname: Yamashita
authors.affiliation: Graduate School of Pharmaceutical Sciences, Osaka University, Osaka, Japan.
authors.forename: Azusa
authors.initials: A
authors.lastname: Nose
authors.affiliation: Graduate School of Pharmaceutical Sciences, Osaka University, Osaka, Japan.
authors.forename: Kaori
authors.initials: K
authors.lastname: Kukino
authors.affiliation: Graduate School of Life Science, University of Hyogo, Hyogo, Japan.
authors.forename: Hitomi
authors.initials: H
authors.lastname: Sawai
authors.affiliation: RIKEN SPring-8 Center, Hyogo, Japan.
authors.forename: Yoshitsugu
authors.initials: Y
authors.lastname: Shiro
authors.affiliation: Graduate School of Pharmaceutical Sciences, Osaka University, Osaka, Japan. Electronic address: unot@phs.osaka-u.ac.jp.
authors.forename: Tadayuki
authors.initials: T
authors.lastname: Uno
mesh_terms: D019943:Amino Acid Substitution; D002248:Carbon Monoxide / Q000737:chemistry; D003553:Cystine / Q000737:chemistry*; D000078842:Cytoglobin; D005914:Globins / Q000737:chemistry* / Q000235:genetics; D006418:Heme / Q000737:chemistry; D006801:Humans; D007700:Kinetics; D008024:Ligands; D016297:Mutagenesis, Site-Directed; D010084:Oxidation-Reduction; D011190:Potassium Cyanide / Q000737:chemistry; D011485:Protein Binding; D055503:Protein Multimerization; D013059:Spectrum Analysis, Raman
chemical_list: C000627265:CYGB protein, human; D000078842:Cytoglobin; D008024:Ligands; D006418:Heme; D003553:Cystine; D002248:Carbon Monoxide; D005914:Globins; D011190:Potassium Cyanide
keywords: Binding affinity; Cytoglobin; Disulfide bond; Resonance Raman spectroscopy