title: In vitro inhibitory effect of miltefosine against strains of Histoplasma capsulatum var. capsulatum and Sporothrix spp.
abstract: Miltefosine (MIL), originally developed for use in cancer chemotherapy, has been shown to have important antifungal activity against several pathogenic fungi. Our aim in this study was to determine the in vitro activity of MIL against the dimorphic fungi Histoplasma capsulatum and Sporothrix spp. This was done using the broth microdilution method. MIL had an in vitro inhibitory effect against all strains of H. capsulatum var. capsulatum and Sporothrix spp. analyzed. The minimal inhibitory concentrations (MIC) varied from 0.25 μg/ml to 2 μg/ml for H. capsulatum var. capsulatum in the filamentous phase and from 0.125 μg/ml to 1 μg/ml in the yeast phase. The MIC interval for Sporothrix spp. in the filamentous phase was 0.25-2 μg/ml. The minimal fungicidal concentrations (MFCs) were ≤4 μg/ml for isolates of both analyzed species. This study demonstrates that MIL has an antifungal effect in vitro against two potentially pathogenic fungi and that more studies should be performed in order to evaluate its applicability in vivo.
authors.affiliation: Specialized Medical Mycology Center, Federal University of Ceará
authors.forename: Raimunda Sâmia Nogueira
authors.initials: RS
authors.lastname: Brilhante
authors.forename: Angela Donato Maia
authors.initials: AD
authors.lastname: Malaquias
authors.forename: Erica Pacheco
authors.initials: EP
authors.lastname: Caetano
authors.forename: Débora de Souza Collares Maia
authors.initials: Dde S
authors.lastname: Castelo-Branco
authors.forename: Rita Amanda Chaves de
authors.initials: RA
authors.lastname: Lima
authors.forename: Francisca Jakelyne de Farias
authors.initials: FJ
authors.lastname: Marques
authors.forename: Natalya Fechine
authors.initials: NF
authors.lastname: Silva
authors.forename: Lucas Pereira de
authors.initials: LP
authors.lastname: Alencar
authors.forename: André Jalles
authors.initials: AJ
authors.lastname: Monteiro
authors.forename: Zoilo Pires de
authors.initials: ZP
authors.lastname: Camargo
authors.forename: Tereza de Jesus Pinheiro Gomes
authors.initials: Tde J
authors.lastname: Bandeira
authors.forename: Anderson Messias
authors.initials: AM
authors.lastname: Rodrigues
authors.forename: Rossana de Aguiar
authors.initials: Rde A
authors.lastname: Cordeiro
authors.forename: José Luciano Bezerra
authors.initials: JL
authors.lastname: Moreira
authors.forename: José Júlio Costa
authors.initials: JJ
authors.lastname: Sidrim
authors.forename: Marcos Fábio Gadelha
authors.initials: MF
authors.lastname: Rocha
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D006658:Histoplasma / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability / Q000187:drug effects; D010767:Phosphorylcholine / Q000031:analogs & derivatives* / Q000494:pharmacology; D013175:Sporothrix / Q000187:drug effects*
chemical_list: D000935:Antifungal Agents; D010767:Phosphorylcholine; C039128:miltefosine
keywords: Histoplasma capsulatum; Sporothrix spp.; inhibitory effect; miltefosine