title: RUNX2 is overexpressed in melanoma cells and mediates their migration and invasion.
abstract: In the present study, we investigated the role of the transcription factor RUNX2 in melanomagenesis. We demonstrated that the expression of transcriptionally active RUNX2 was increased in melanoma cell lines as compared with human melanocytes. Using a melanoma tissue microarray, we showed that RUNX2 levels were higher in melanoma cells as compared with nevic melanocytes. RUNX2 knockdown in melanoma cell lines significantly decreased Focal Adhesion Kinase expression, and inhibited their cell growth, migration and invasion ability. Finally, the pro-hormone cholecalciferol reduced RUNX2 transcriptional activity and decreased migration of melanoma cells, further suggesting a role of RUNX2 in melanoma cell migration.
authors.affiliation: Rutgers Cancer Institute of New Jersey, Department of Medicine, Division of Medical Oncology - Rutgers, The State University of New Jersey, Robert Wood Johnson Medical School, 195 Little Albany Street, New Brunswick, NJ 08903, USA.
authors.forename: Rajeev K
authors.initials: RK
authors.lastname: Boregowda
authors.affiliation: Rutgers Cancer Institute of New Jersey, Department of Medicine, Division of Medical Oncology - Rutgers, The State University of New Jersey, Robert Wood Johnson Medical School, 195 Little Albany Street, New Brunswick, NJ 08903, USA.
authors.forename: Oyenike O
authors.initials: OO
authors.lastname: Olabisi
authors.affiliation: Rutgers Cancer Institute of New Jersey, Department of Medicine, Division of Medical Oncology - Rutgers, The State University of New Jersey, Robert Wood Johnson Medical School, 195 Little Albany Street, New Brunswick, NJ 08903, USA.
authors.forename: Walid
authors.initials: W
authors.lastname: Abushahba
authors.affiliation: Rutgers Cancer Institute of New Jersey, Department of Surgery, Division of Surgical Oncology, Rutgers, The State University of New Jersey, Robert Wood Johnson Medical School, 195 Little Albany Street, New Brunswick, NJ 08903, USA.
authors.forename: Byeong-Seon
authors.initials: BS
authors.lastname: Jeong
authors.affiliation: Rutgers Cancer Institute of New Jersey, Department of Surgery, Division of Surgical Oncology, Rutgers, The State University of New Jersey, Robert Wood Johnson Medical School, 195 Little Albany Street, New Brunswick, NJ 08903, USA.
authors.forename: Keneshia K
authors.initials: KK
authors.lastname: Haenssen
authors.affiliation: Center for Biomedical Imaging & Informatics - Rutgers Cancer Institute of New Jersey, 195 Little Albany Street, New Brunswick, NJ 08903, USA.
authors.forename: Wenjin
authors.initials: W
authors.lastname: Chen
authors.affiliation: Rutgers Cancer Institute of New Jersey - Department of Pathology and Laboratory Medicine, Robert Wood Johnson University Hospital, 1 RWJ Place, New Brunswick, NJ 08901, USA.
authors.forename: Marina
authors.initials: M
authors.lastname: Chekmareva
authors.affiliation: Department of Pharmacology and Toxicology, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, 160 Frelinghuysen Road, Piscataway, NJ 08854, USA.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Lasfar
authors.affiliation: Center for Biomedical Imaging & Informatics - Rutgers Cancer Institute of New Jersey, 195 Little Albany Street, New Brunswick, NJ 08903, USA.
authors.forename: David J
authors.initials: DJ
authors.lastname: Foran
authors.affiliation: Rutgers Cancer Institute of New Jersey, Department of Surgery, Division of Surgical Oncology, Rutgers, The State University of New Jersey, Robert Wood Johnson Medical School, 195 Little Albany Street, New Brunswick, NJ 08903, USA.
authors.forename: James S
authors.initials: JS
authors.lastname: Goydos
authors.affiliation: Rutgers Cancer Institute of New Jersey, Department of Medicine, Division of Medical Oncology - Rutgers, The State University of New Jersey, Robert Wood Johnson Medical School, 195 Little Albany Street, New Brunswick, NJ 08903, USA. Electronic address: cohenska@cinj.rutgers.edu.
authors.forename: Karine A
authors.initials: KA
authors.lastname: Cohen-Solal
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation; D002762:Cholecalciferol / Q000494:pharmacology; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics / Q000378:metabolism*; D051417:Focal Adhesion Kinase 1 / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D053509:Matrix Metalloproteinase 13 / Q000235:genetics; D008545:Melanoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D009361:Neoplasm Invasiveness; D011401:Promoter Regions, Genetic; D034622:RNA Interference; D015398:Signal Transduction; D012878:Skin Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D046888:Tissue Array Analysis; D014158:Transcription, Genetic; D014162:Transfection; D015854:Up-Regulation
chemical_list: D050659:Core Binding Factor Alpha 1 Subunit; C493709:RUNX2 protein, human; D002762:Cholecalciferol; D051417:Focal Adhesion Kinase 1; C496050:PTK2 protein, human; D053509:Matrix Metalloproteinase 13
keywords: Cholecalciferol (Vitamin D3); FAK; Melanoma; Migration; RUNX2; Transcription factor