title: Crystallization and preliminary X-ray analysis of the C-type lectin domain of the spicule matrix protein SM50 from Strongylocentrotus purpuratus.
abstract: Sea urchin spicules have a calcitic mesocrystalline architecture that is closely associated with a matrix of proteins and amorphous minerals. The mechanism underlying spicule formation involves complex processes encompassing spatio-temporally regulated organic-inorganic interactions. C-type lectin domains are present in several spicule matrix proteins in Strongylocentrotus purpuratus, implying their role in spiculogenesis. In this study, the C-type lectin domain of SM50 was overexpressed, purified and crystallized using a vapour-diffusion method. The crystal diffracted to a resolution of 2.85 Å and belonged to space group P212121, with unit-cell parameters a = 100.6, b = 115.4, c = 130.6 Å, α = β = γ = 90°. Assuming 50% solvent content, six chains are expected to be present in the asymmetric unit.
authors.affiliation: Department of Biology, University of Konstanz, Universitätsstrasse 10, 78457 Konstanz, Germany.
authors.forename: Puneet
authors.initials: P
authors.lastname: Juneja
authors.affiliation: Department of Chemistry, University of Konstanz, Universitätsstrasse 10, 78457 Konstanz, Germany.
authors.forename: Ashit
authors.initials: A
authors.lastname: Rao
authors.affiliation: Department of Chemistry, University of Konstanz, Universitätsstrasse 10, 78457 Konstanz, Germany.
authors.forename: Helmut
authors.initials: H
authors.lastname: Cölfen
authors.affiliation: Department of Biology, University of Konstanz, Universitätsstrasse 10, 78457 Konstanz, Germany.
authors.forename: Kay
authors.initials: K
authors.lastname: Diederichs
authors.affiliation: Department of Biology, University of Konstanz, Universitätsstrasse 10, 78457 Konstanz, Germany.
authors.forename: Wolfram
authors.initials: W
authors.lastname: Welte
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D016326:Extracellular Matrix Proteins / Q000737:chemistry* / Q000302:isolation & purification; D037181:Lectins, C-Type / Q000737:chemistry*; D008969:Molecular Sequence Data; D047270:Strongylocentrotus purpuratus / Q000737:chemistry*
chemical_list: D016326:Extracellular Matrix Proteins; D037181:Lectins, C-Type; C051691:SM50 protein, sea urchin
keywords: C-type lectins; SM50; Strongylocentrotus purpuratus; spiculogenesis