title: Microsomal protein per gram of liver (MPPGL) in paediatric biliary atresia patients.
abstract: The microsomal protein per gram of liver (MPPGL) is an important scaling factor in the in vitro-in vivo extrapolation of metabolic data obtained in liver microsomes. This study aimed to determine the MPPGL in four biliary atresia patients (0.6-1.6 years old) undergoing liver transplantation, as it is known that the MPPGL is affected by age and possibly by liver disease. Due to the presence of bilirubin in the homogenates and microsomes, the NADPH-cytochrome reductase activity was used to determine the recovery factor, rather than methods using the dithionite difference spectrum. A mean value of 18.73 (± 2.82) mg/g (geometric mean ± SD, n = 4) was observed, which is lower than the expected MPPGL based on the age of the patients (26.60 ± 0.40 mg/g). This suggests a decreased amount of microsomal protein in the livers of biliary atresia patients. Moreover, no differences in MPPGL between different zones of the liver could be detected.
authors.affiliation: Laboratory of Medical Biochemistry and Clinical Analysis, Faculty of Pharmaceutical Sciences, Ghent University, Harelbekestraat 72, 9000, Ghent, Belgium.
authors.forename: Lies
authors.initials: L
authors.lastname: De Bock
authors.forename: Koen
authors.initials: K
authors.lastname: Boussery
authors.forename: Ruth
authors.initials: R
authors.lastname: De Bruyne
authors.forename: Myriam
authors.initials: M
authors.lastname: Van Winckel
authors.forename: Xavier
authors.initials: X
authors.lastname: Stephenne
authors.forename: Etienne
authors.initials: E
authors.lastname: Sokal
authors.forename: Jan
authors.initials: J
authors.lastname: Van Bocxlaer
mesh_terms: D000367:Age Factors; D001656:Biliary Atresia / Q000503:physiopathology*; D001663:Bilirubin / Q000378:metabolism; D006801:Humans; D007223:Infant; D008099:Liver / Q000378:metabolism* / Q000601:surgery; D016031:Liver Transplantation; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism*; D009251:NADPH-Ferrihemoprotein Reductase / Q000378:metabolism; D011506:Proteins / Q000378:metabolism
chemical_list: D011506:Proteins; D009251:NADPH-Ferrihemoprotein Reductase; D001663:Bilirubin
keywords: biliary atresia; cytochrome P450; in vitro-in vivo extrapolation (IVIVE); microsomal protein per gram liver (MPPGL)