title: The effect of combined regulation of the expression of peroxisome proliferator-activated receptor-γ and calcitonin gene-related peptide on alcohol-induced adipogenic differentiation of bone marrow mesenchymal stem cells.
abstract: Studies have shown that alcohol can upregulate the expression of peroxisome proliferator-activated receptor-γ (PPARγ) gene in bone marrow mesenchymal stem cells (BMSCs). High expression of PPARγ can promote adipogenic differentiation of BMSCs, and reduce their osteogenic differentiation. Abnormal proliferation of adipocytes and fatty accumulation in osteocytes can result in high intraosseous pressure and disturbance of blood circulation in the femoral head, which induces osteonecrosis of the femoral head (ONFH). Downregulation of PPARγ is efficient in inhibiting adipogenesis and maintaining osteogenesis of BMSCs, which might potentially reduce the incidence of ONFH. Calcitonin gene-related peptide (CGRP) is a neuropeptide gene which has been closely associated with bone regeneration. In this study, we aimed to observe the effect of combined regulation of the expression of PPARγ and CGRP genes on alcohol-induced adipogenic differentiation of BMSCs. Our results demonstrated that simultaneous downregulation of PPARγ and upregulation of CGRP was efficient in suppressing adipogenic differentiation of BMSCs and promoting their osteogenic differentiation. These findings might enlighten a novel approach for the prevention of ONFH.
authors.affiliation: Department of Orthopaedic Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, Henan, China.
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Li
authors.forename: Yisheng
authors.initials: Y
authors.lastname: Wang
authors.forename: Yuebai
authors.initials: Y
authors.lastname: Li
authors.forename: Junkui
authors.initials: J
authors.lastname: Sun
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Zhao
mesh_terms: D017667:Adipocytes / Q000166:cytology*; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000235:genetics*; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D024042:Collagen Type I / Q000378:metabolism; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics; D003470:Culture Media; D000431:Ethanol / Q000494:pharmacology*; D007797:Laminin / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D015675:Osteocalcin / Q000235:genetics; D047495:PPAR gamma / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D011817:Rabbits; D014280:Triglycerides / Q000378:metabolism
chemical_list: D024042:Collagen Type I; D050659:Core Binding Factor Alpha 1 Subunit; D003470:Culture Media; D007797:Laminin; D047495:PPAR gamma; D012333:RNA, Messenger; C493716:Runx2 protein, mouse; D014280:Triglycerides; D015675:Osteocalcin; D000431:Ethanol; D000469:Alkaline Phosphatase; D015740:Calcitonin Gene-Related Peptide