title: A dispersive liquid--liquid microextraction methodology for copper (II) in environmental samples prior to determination using microsample injection flame atomic absorption spectrometry.
abstract: A simple, environmentally friendly, and efficient dispersive liquid-liquid microextraction method combined with microsample injection flame atomic absorption spectrometry was developed for the separation and preconcentration of Cu(II). 2-(5-Bromo-2-pyridylazo)-5-(diethylamino)phenol (5-Br-PADAP) was used to form a hydrophobic complex of Cu(II) ions in the aqueous phase before extraction. To extract the Cu(II)-5-Br-PADAP complex from the aqueous phase to the organic phase, 2.0 mL of acetone as a disperser solvent and 200 microL of chloroform as an extraction solvent were used. The influences of important analytical parameters, such as the pH, types and volumes of the extraction and disperser solvents, amount of chelating agent, sample volume, and matrix effects, on the microextraction procedure were evaluated and optimized. Using the optimal conditions, the LOD, LOQ, preconcentration factor, and RSD were determined to be 1.4 microg/L, 4.7 microg/L, 120, and 6.5%, respectively. The accuracy of the proposed method was investigated using standard addition/recovery tests. The analysis of certified reference materials produced satisfactory analytical results. The developed method was applied for the determination of Cu in real samples.
authors.forename: Zeid A
authors.initials: ZA
authors.lastname: Alothman
authors.forename: Mohamed
authors.initials: M
authors.lastname: Habila
authors.forename: Erkan
authors.initials: E
authors.lastname: Yilmaz
authors.forename: Mustafa
authors.initials: M
authors.lastname: Soylak
mesh_terms: D000042:Absorption; D001391:Azo Compounds / Q000737:chemistry; D001628:Beverages / Q000032:analysis; D002614:Chelating Agents / Q000737:chemistry; D002623:Chemistry Techniques, Analytical; D002725:Chloroform / Q000737:chemistry; D003300:Copper / Q000032:analysis* / Q000737:chemistry*; D005504:Food Analysis / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D007477:Ions; D059627:Liquid Phase Microextraction / Q000379:methods*; D015203:Reproducibility of Results; D012997:Solvents / Q000737:chemistry; D013054:Spectrophotometry, Atomic / Q000379:methods*; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D001391:Azo Compounds; D002614:Chelating Agents; D007477:Ions; D012997:Solvents; D014874:Water Pollutants, Chemical; C037882:2-(5-bromo-2-pyridylazo)-5-diethylaminophenol; D003300:Copper; D002725:Chloroform