title: Diagnosis of liver cancer from blood sera using FTIR microspectroscopy: a preliminary study.
abstract: FTIR microspectroscopy was applied for studying macromolecular changes in human serum samples from patients with healthy livers, and those diagnosed with liver cirrhosis or hepatocellular carcinoma (HCC). Our study demonstrated that the serum samples from HCC and cirrhotic patients could readily be discriminated from those from healthy controls based on macromolecular differences related to their lipid and protein structure. Spectral changes appeared to indicate that the secondary structure of protein from HCC sample groups contained a more distinctive β -sheet structure and a lower lipid content compared to samples from the healthy and cirrhosis group. This was correlated with measurements of large decreases in albumin levels in serum from diseased patients. We argue that this technique shows potential as a simple, rapid, inexpensive, and non-subjective methodology for the screening patients suspected of liver disease.
authors.affiliation: Synchrotron Light Research Institute public organization, Muang, Nakhonratchasima, 30000, Thailand.
authors.forename: Kanjana
authors.initials: K
authors.lastname: Thumanu
authors.forename: Suleeporn
authors.initials: S
authors.lastname: Sangrajrang
authors.forename: Thiravud
authors.initials: T
authors.lastname: Khuhaprema
authors.forename: Anant
authors.initials: A
authors.lastname: Kalalak
authors.forename: Waraporn
authors.initials: W
authors.lastname: Tanthanuch
authors.forename: Siwatt
authors.initials: S
authors.lastname: Pongpiachan
authors.forename: Philip
authors.initials: P
authors.lastname: Heraud
mesh_terms: D014408:Biomarkers, Tumor / Q000097:blood*; D001706:Biopsy; D006528:Carcinoma, Hepatocellular / Q000097:blood* / Q000175:diagnosis*; D016000:Cluster Analysis; D006801:Humans; D008055:Lipids / Q000097:blood; D008099:Liver / Q000201:enzymology; D008103:Liver Cirrhosis / Q000097:blood* / Q000175:diagnosis*; D008113:Liver Neoplasms / Q000097:blood* / Q000175:diagnosis*; D015999:Multivariate Analysis; D025341:Principal Component Analysis; D017433:Protein Structure, Secondary; D012709:Serum Albumin / Q000032:analysis; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
chemical_list: D014408:Biomarkers, Tumor; D008055:Lipids; D012709:Serum Albumin
keywords: FTIR microspectroscopy; blood serum; hepatocellular carcinoma (HCC); liver cirrhosis