title: Serological screening of the Schistosoma mansoni adult worm proteome.
abstract: BACKGROUND
  New interventions tools are a priority for schistosomiasis control and elimination, as the disease is still highly prevalent. The identification of proteins associated with active infection and protective immune response may constitute the basis for the development of a successful vaccine and could also indicate new diagnostic candidates. In this context, post-genomic technologies have been progressing, resulting in a more rational discovery of new biomarkers of resistance and antigens for diagnosis.
  
  
  RESULTS
  Two-dimensional electrophoresed Schistosoma mansoni adult worm protein extracts were probed with pooled sera of infected and non-infected (naturally resistant) individuals from a S. mansoni endemic area. A total of 47 different immunoreactive proteins were identified by mass spectrometry. Although the different pooled sera shared most of the immunoreactive protein spots, nine protein spots reacted exclusively with the serum pool of infected individuals, which correspond to annexin, major egg antigen, troponin T, filamin, disulphide-isomerase ER-60 precursor, actin and reticulocalbin. One protein spot, corresponding to eukaryotic translation elongation factor, reacted exclusively with the pooled sera of non-infected individuals living in the endemic area. Western blotting of two selected recombinant proteins, major egg antigen and hemoglobinase, showed a similar recognition pattern of that of the native protein.
  Using a serological proteome analysis, a group of antigens related to the different infection status of the endemic area residents was identified and may be related to susceptibility or resistance to infection.
authors.affiliation: Centro de Pesquisas René Rachou-Fiocruz/MG, Genomics and Computational Biology Group, Belo Horizonte, Minas Gerais, Brazil; National Institute of Science and Technology in Tropical Diseases - INCT-DT, Brazil.
authors.forename: Fernanda
authors.initials: F
authors.lastname: Ludolf
authors.affiliation: Centro de Pesquisas René Rachou-Fiocruz/MG, Genomics and Computational Biology Group, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Paola R
authors.initials: PR
authors.lastname: Patrocínio
authors.affiliation: National Institute of Science and Technology in Tropical Diseases - INCT-DT, Brazil; Centro de Pesquisas René Rachou-Fiocruz/MG, Cellular and Molecular Immunology Laboratory, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Corrêa-Oliveira
authors.affiliation: National Institute of Science and Technology in Tropical Diseases - INCT-DT, Brazil; Universidade Federal de Minas Gerais, Nursing School, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Andréa
authors.initials: A
authors.lastname: Gazzinelli
authors.affiliation: The University of Nottingham, School of Pharmacy, Division of Molecular and Cellular Science, Nottingham, East Midlands, United Kingdom.
authors.forename: Franco H
authors.initials: FH
authors.lastname: Falcone
authors.affiliation: Fiocruz/RJ, Department of Physiology and Pharmacodynamics, Toxicology Laboratory, Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: André
authors.initials: A
authors.lastname: Teixeira-Ferreira
authors.affiliation: Fiocruz/RJ, Department of Physiology and Pharmacodynamics, Toxicology Laboratory, Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Jonas
authors.initials: J
authors.lastname: Perales
authors.affiliation: Centro de Pesquisas René Rachou-Fiocruz/MG, Genomics and Computational Biology Group, Belo Horizonte, Minas Gerais, Brazil; National Institute of Science and Technology in Tropical Diseases - INCT-DT, Brazil.
authors.forename: Guilherme C
authors.initials: GC
authors.lastname: Oliveira
authors.affiliation: Centro de Pesquisas René Rachou-Fiocruz/MG, Genomics and Computational Biology Group, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Rosiane A
authors.initials: RA
authors.lastname: Silva-Pereira
mesh_terms: D000328:Adult; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood* / Q000276:immunology*; D000947:Antigens, Helminth / Q000032:analysis* / Q000276:immunology*; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D015151:Immunoblotting; D008297:Male; D013058:Mass Spectrometry; D020543:Proteome / Q000032:analysis*; D012550:Schistosoma mansoni / Q000276:immunology*; D055815:Young Adult
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D020543:Proteome