title: Alarms and parameters generated by hematology analyzer: new tools to predict and quantify circulating Sezary cells.
abstract: BACKGROUND
  The rigorous cytological review by manual or automatic microscopic analysis is critical in the detection of circulating neoplastic cells, since their morphology as well as their count contributes to the diagnosis and prognosis of many diseases. However, the cytological analysis is not always obvious and requires trained and competent cytologist. In this context, the alarms and/or parameters generated by hematology analyzer could be particularly informative to alert the operators.
  
  
  METHODS
  Blood samples from patients with Sezary syndrome (n = 9) were studied with Sysmex XN-1000 analyzer, and compared to patients with benign or tumoral skin lesions (n = 47) and patients with chronic lymphoproliferative B-cell diseases (n = 51) used as control.
  
  
  RESULTS
  In present series, the value of structural lymphoid parameters (LyX and LyZ) and the alarm Blast/Abn Lympho were statistically higher in Sezary cases than in control cases. In addition, the value of LyX was associated to the count of circulating Sezary cells and value of LyZ to the presence of large Sezary cells, both parameters described as prognostic factors.
  
  
  CONCLUSIONS
  The combination of alarm Blast/Abn Lympho and structural parameters (Ly-X/Ly-Z/Ly-Y) may allow to define rule of blood slide review to screen circulating Sezary cells, and give promising results in B-cell diseases.
authors.affiliation: Laboratoire d'Hématologie Cellulaire, Centre Hospitalier Lyon-Sud, Hospices Civils de Lyon, Pierre-Bénite, France.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Brisou
authors.forename: Delphine
authors.initials: D
authors.lastname: Manzoni
authors.forename: Stephane
authors.initials: S
authors.lastname: Dalle
authors.forename: Pascale
authors.initials: P
authors.lastname: Felman
authors.forename: Dominique
authors.initials: D
authors.lastname: Morel
authors.forename: Marouane
authors.initials: M
authors.lastname: Boubaya
authors.forename: Jean Pierre
authors.initials: JP
authors.lastname: Magaud
authors.forename: Lucile
authors.initials: L
authors.lastname: Baseggio
mesh_terms: D001322:Autoanalysis; D001402:B-Lymphocytes / Q000473:pathology; D001772:Blood Cell Count; D002467:Cell Nucleus / Q000473:pathology; D003593:Cytoplasm / Q000473:pathology; D003937:Diagnosis, Differential; D005260:Female; D006403:Hematologic Tests / Q000295:instrumentation*; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000097:blood; D008297:Male; D008875:Middle Aged; D012751:Sezary Syndrome / Q000097:blood*; D013601:T-Lymphocytes / Q000473:pathology*
keywords: diagnosis; morphology; prognosis; sezary