title: Performance of a rapid multi-analyte 2-photon excitation assay in children with acute respiratory infection.
abstract: The purpose of this study is to evaluate the diagnostic performance of the novel 2-photon excitation-based mariPOC© Assay (ArcDia Laboratories, Turku, Finland) for antigen detection of respiratory viruses versus real-time polymerase chain reaction (PCR). The mariPOC Assay and 2 multiplex real-time PCR techniques were performed on nasopharyngeal samples from pediatric patients with suspicion of acute respiratory infection admitted to a children's hospital in Spain during October 2011 to January 2013. A total of 233 samples were studied. Sensitivities and specificities (95% confidence interval) of the mariPOC Assay were for respiratory syncytial virus (RSV), 78.4% (69.7-85.6) and 99.2% (96.3-100.0); influenza virus (IFV) A, 66.7% (26.2-94.0) and 99.6% (97.9-100.0); IFV-B, 63.6% (33.6-87.2) and 100.0% (98.7-100.0); human metapneumovirus (hMPV), 60.0% (34.5-81.9) and 100.0% (98.6-100.0); adenovirus (ADV), 12.5% (0.6-48.0) and 100.0% (98.7-100.0), respectively. The mariPOC Assay is a highly specific method for simultaneous detection of 8 respiratory viruses but has sensitivities that range from moderately high for RSV to moderate for IFV and hMPV and low for ADV.
authors.affiliation: Department of Microbiology, Hospital Sant Joan de Deu and University of Barcelona, Passeig Sant Joan de Deu, 08950 Esplugues, Barcelona, Spain.
authors.forename: Pedro
authors.initials: P
authors.lastname: Brotons
authors.affiliation: Department of Paediatrics, Hospital Sant Joan de Deu and University of Barcelona, Passeig Sant Joan de Deu, 08950 Esplugues, Barcelona, Spain.
authors.forename: Cristian
authors.initials: C
authors.lastname: Launes
authors.affiliation: Department of Microbiology, Hospital Sant Joan de Deu and University of Barcelona, Passeig Sant Joan de Deu, 08950 Esplugues, Barcelona, Spain.
authors.forename: Melania
authors.initials: M
authors.lastname: Iñigo
authors.affiliation: Department of Microbiology, Hospital Sant Joan de Deu and University of Barcelona, Passeig Sant Joan de Deu, 08950 Esplugues, Barcelona, Spain.
authors.forename: Natalia
authors.initials: N
authors.lastname: Peris
authors.affiliation: Department of Microbiology, Hospital Sant Joan de Deu and University of Barcelona, Passeig Sant Joan de Deu, 08950 Esplugues, Barcelona, Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: Selva
authors.affiliation: Department of Microbiology, Hospital Sant Joan de Deu and University of Barcelona, Passeig Sant Joan de Deu, 08950 Esplugues, Barcelona, Spain. Electronic address: cma@hsjdbcn.org.
authors.forename: Carmen
authors.initials: C
authors.lastname: Muñoz-Almagro
mesh_terms: D000293:Adolescent; D000956:Antigens, Viral / Q000032:analysis*; D002648:Child; D002675:Child, Preschool; D019411:Clinical Laboratory Techniques / Q000379:methods*; D006776:Hospitals, Pediatric; D006801:Humans; D007118:Immunoassay / Q000379:methods; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods; D009305:Nasopharynx / Q000821:virology; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D012141:Respiratory Tract Infections / Q000175:diagnosis*; D012680:Sensitivity and Specificity; D013030:Spain; D014777:Virus Diseases / Q000175:diagnosis*
chemical_list: D000956:Antigens, Viral
keywords: Antigen detection; Children; Point-of-care; Rapid testing; Respiratory infection