title: Expression ofsetis downregulated by rapamycin in human colorectal cancer cells.
abstract: The purpose of this study was to determine the mechanism through which rapamycin treatment affects the expression of thesetgene in human colorectal adenocarcinoma cells. The effect of rapamycin treatment onsetexpression was evaluated by assessing the mRNA and protein expression ofsetin the SW480 and LoVo human colon carcinoma cell lines following treatment with rapamycin by quantitative polymerase chain reaction (qPCR) and western blot analysis, respectively. Our results demonstrated that the mRNA and protein levels ofsetwere significantly decreased subsequent to rapamycin treatment in the two cell lines, indicating thatsetexpression may be downregulated by rapamycin in human colorectal adenocarcinoma cells. Our findings suggested that the mammalian target of rapamycin signaling pathway may play a role in tumorigenesis through the regulation of thesetgene.
authors.affiliation: Department of Anatomy, College of Basic and Forensic Medicine, Sichuan University, Chengdu, Sichuan 610041, P.R. China.
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Wen
authors.affiliation: Department of Anatomy, College of Basic and Forensic Medicine, Sichuan University, Chengdu, Sichuan 610041, P.R. China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Chen
keywords: human colorectal adenocarcinoma; mammalian target of rapamycin; rapamycin; set