title: Circular permutation of chicken interleukin-1 beta enhances its thermostability.
abstract: Interleukin-1β is a cytokine critically involved in immune and inflammatory responses. To extend its use as a component of avian vaccines, a circularly permuted chicken interleukin-1β was synthesized that maintains its activity after pre-incubation at high temperatures, unlike wild-type chicken interleukin-1β, which is irreversibly inactivated at high temperatures.
authors.affiliation: Institute of Bioinformatics and Structural Biology, National Tsing Hua University, Hsinchu, Taiwan. hstin@mx.nthu.edu.tw.
authors.forename: Wen-Ting
authors.initials: WT
authors.lastname: Chen
authors.forename: Ting
authors.initials: T
authors.lastname: Chen
authors.forename: Chao-Sheng
authors.initials: CS
authors.lastname: Cheng
authors.forename: Wen-Yang
authors.initials: WY
authors.lastname: Huang
authors.forename: Xinquan
authors.initials: X
authors.lastname: Wang
authors.forename: Hsien-Sheng
authors.initials: HS
authors.lastname: Yin
mesh_terms: D000818:Animals; D002645:Chickens / Q000276:immunology / Q000378:metabolism*; D002942:Circular Dichroism; D005453:Fluorescence; D006358:Hot Temperature; D053583:Interleukin-1beta / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D009154:Mutation / Q000235:genetics; D055550:Protein Stability; D017472:Receptors, Interleukin-1 / Q000378:metabolism; D020349:Surface Plasmon Resonance
chemical_list: D053583:Interleukin-1beta; D017472:Receptors, Interleukin-1