title: Rapid and sensitive headspace gas chromatography-mass spectrometry method for the analysis of ethanol in the whole blood.
abstract: BACKGROUND
  For the forensic aim, a sensitive and specific method using headspace gas chromatography coupled with mass spectrometry (GC/MS) has been developed for the quantitative determination of ethanol in blood using n-propanol as internal standard. GC was performed in isothermal mode with a GC run-time of 5.0 min.
  
  
  METHODS
  The quantification was performed using selected ions monitoring mode adopting a quantitative ion and qualifier ion for ethanol and the internal standard.
  
  
  RESULTS
  The method was linear (r(2) = 0.999, in the concentration range of 39.5-1,262.9 μg/ml), specific, sensitive (limit of quantification and limit of detection of 39.5 and 0.4 μg/ml, respectively), and robust. A slightly modified method was also developed for the quantification of 50 commonly abused drunken in blood. The method used an isothermal GC program with a run-time of 5.0 min. The quantification was performed using selected ions monitoring mode and integrating the area under the peak using n-propanol as an internal standard. The method was linear 40-1,263 μg/ml and sensitive.
  
  
  CONCLUSIONS
  The method was proved superior in speed and selectivity to previously reported methods and was successfully applied to the pharmacokinetic study of ethanol.
authors.affiliation: Department of Pharmacy, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, Chengdu, China.
authors.forename: Hong-tao
authors.initials: HT
authors.lastname: Xiao
authors.forename: Lin
authors.initials: L
authors.lastname: He
authors.forename: Rong-sheng
authors.initials: RS
authors.lastname: Tong
authors.forename: Ji-ying
authors.initials: JY
authors.lastname: Yu
authors.forename: Lu
authors.initials: L
authors.lastname: Chen
authors.forename: Jing
authors.initials: J
authors.lastname: Zou
authors.forename: Jin-qi
authors.initials: JQ
authors.lastname: Li
authors.forename: Yuan
authors.initials: Y
authors.lastname: Bian
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000433:1-Propanol / Q000097:blood; D000431:Ethanol / Q000097:blood*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D057230:Limit of Detection; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D000431:Ethanol; D000433:1-Propanol
keywords: GC-MS; ethanol; headspace; n-propanol; whole blood