title: Non-intercalative, deoxyribose binding of boric acid to calf thymus DNA.
abstract: The present study characterizes the effects of the boric acid binding on calf thymus DNA (ct-DNA) by spectroscopic and calorimetric methods. UV-Vis absorbance spectroscopy, circular dichroism (CD) spectroscopy, transmission electron microscopy (TEM), isothermal titration calorimetry (ITC), and Fourier transform infrared (FT-IR) spectroscopy were employed to characterize binding properties. Changes in the secondary structure of ct-DNA were determined by CD spectroscopy. Sizes and morphologies of boric acid-DNA complexes were determined by transmission electron microscopy (TEM). The kinetics of boric acid binding to calf thymus DNA (ct-DNA) was investigated by isothermal titration calorimetry (ITC). ITC results revealed that boric acid exhibits a moderate affinity to ct-DNA with a binding constant (K a) of 9.54 × 10(4) M(-1). FT-IR results revealed that boric acid binds to the deoxyribose sugar of DNA without disrupting the B-conformation at tested concentrations.
authors.affiliation: UNAM-National Nanotechnology Research Center, Bilkent University, Ankara, Bilkent, 06800, Turkey.
authors.forename: Ayse
authors.initials: A
authors.lastname: Ozdemir
authors.forename: Refiye Tekiner
authors.initials: RT
authors.lastname: Gursaclı
authors.forename: Turgay
authors.initials: T
authors.lastname: Tekinay
mesh_terms: D000818:Animals; D001665:Binding Sites; D001888:Boric Acids / Q000737:chemistry*; D002151:Calorimetry; D002417:Cattle; D004247:DNA / Q000737:chemistry*; D003855:Deoxyribose / Q000737:chemistry*
chemical_list: D001888:Boric Acids; D003855:Deoxyribose; D004247:DNA; C427203:calf thymus DNA; C032688:boric acid