title: Interferon-gamma gene polymorphism +874 (a/t) in Chinese children with Henoch-Schonlein purpura.
abstract: The aim of this study was to investigate the possible influence of Interferon-gamma (IFN-γ) gene polymorphism +874 (A/T) (rs2430561) in the susceptibility and renal complications of patients with Henoch-Schonlein purpura (HSP). We also studied the effects of IFN-γ allelic variation on serum levels of pro-and anti-inflammatory cytokines in HSP patients. The study population comprised 97 patients suffering from HSP and 97 control participants. Patients and controls were genotyped for a single nucleotide polymorphism +874 (A/T) in the first intron of the IFN-γ gene by the TaqMan PCR method. Frequencies of individuals with IFN-γ +874 AA, AT and TT genotypes were 77.3%, 21.6% and 1% in HSP patients and 79.4%, 17.5% and 3.1% in controls, respectively. The frequency of the AA genotype in HSP patients with nephritis was slightly higher (83.3%) than in HSP patients without nephritis (73.8%). The allele A occurred more commonly in HSP patients with nephritis (92%) than in HSP patients without nephritis (86%), but these differences were not statistically significant (p= 0.469 and p= 0.244, respectively). In addition, significant difference in serum IL-10 levels between IFN-γ +874 different genotype groups was found. Our results do not support a role for IFN-γ gene polymorphism +874 (A/T) in the susceptibility to HSP and allelic variation at IFN-γ +874 locus had no effect on serum levels of cytokines in patients with HSP except for IL-10.
authors.affiliation: Department of Clinical Laboratory, Children's Hospital of Zhejiang University School of Medicine, Hangzhou, P.R. China. xuh32323@163.com.
authors.forename: Hui
authors.initials: H
authors.lastname: Xu
authors.affiliation: Department of Clinical Laboratory, Children's Hospital of Zhejiang University School of Medicine, Hangzhou, P.R. China. liwei19860607@163.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Nephrology, Children's Hospital of Zhejiang University School of Medicine, Hangzhou, P.R. China. haidong@126.com.
authors.forename: Haidong
authors.initials: H
authors.lastname: Fu
authors.affiliation: Department of Clinical Laboratory, Children's Hospital of Zhejiang University School of Medicine, Hangzhou, P.R. China. guizhengjiang@126.com.
authors.forename: Guizheng
authors.initials: G
authors.lastname: Jiang
mesh_terms: D000293:Adolescent; D044466:Asian People / Q000235:genetics; D002648:Child; D002675:Child, Preschool; D005260:Female; D005838:Genotype; D006801:Humans; D011695:IgA Vasculitis / Q000235:genetics* / Q000276:immunology; D007371:Interferon-gamma / Q000235:genetics*; D016753:Interleukin-10 / Q000097:blood; D008297:Male; D020641:Polymorphism, Single Nucleotide
chemical_list: D016753:Interleukin-10; D007371:Interferon-gamma