title: Efficacy of halofuginone lactate against experimental cryptosporidiosis in goat neonates.
abstract: Preliminary results obtained in calves, lambs and goat kids infected by Cryptosporidium sp. have indicated a partial prophylactic efficacy of halofuginone lactate when administered at 100 μg/kg body weight (BW). In this study, the efficacy of halofuginone lactate was evaluated in goat neonates experimentally inoculated with Cryptosporidium parvum oocysts per oral route. The trial consisted in 2 replicated experiments carried out successively at 2 months of interval. Twenty-two 2- to 4-day-old kids were experimentally inoculated once, 2-3 days after the arrival in premises, with 10(6)C. parvum oocysts per oral route and were allocated into 2 groups. Animals of group 1 acted as untreated control whereas animals of group 2 received halofuginone lactate for 10 days from the infection day to day 9 post-infection (DPI) at a daily oral dose rate of 100 μg/kg BW. Individual oocyst shedding was monitored by daily examination of faecal smears stained by carbol fuchsin and scored semi-quantitatively (0-5) until 19 DPI. Daily diarrhoea scores, weight gain and mortality were recorded. In the first experiment, oocyst excretion started 1 DPI in the control group, was highest on 4 DPI (mean score 3.6) and became undetectable from 16-19 DPI. In the treated group, oocyst shedding started 1 day later, showed lower scores compared to control on 4, 5, 6, 7 and 10 DPI and vanished from 16 to 19 DPI. No significant difference was seen for weight gains between groups. Five kids died in the control group compared to 1 kid in the treated group. In the second (replicated) experiment, oocyst excretion started 2 DPI in the control group, was highest on 4 DPI (mean score 4.5) and became undetectable 18 and 19 DPI. In the treated group, oocyst shedding started 2 days later, peaked on 13 DPI (mean score 2.3) and persisted until the end of the experiment. No significant difference was seen for weight gains between groups. Ten kids died in the control group compared to 3 kids in the treated group. The results demonstrated the efficacy of halofuginone lactate when given as a prophylactic treatment at 100 μg/kg BW during 10 days in reducing oocyst shedding, diarrhoea and mortality in goat kid cryptosporidiosis.
authors.affiliation: LUNAM Université, Oniris, Nantes-Atlantic College of Veterinary Medicine and Food Sciences and Engineering, F-44307 Nantes, France; Anses Niort Laboratory, French Agency for Food, Environmental and Occupational Health & Safety, F-79024 Niort, France.
authors.forename: Julie
authors.initials: J
authors.lastname: Petermann
authors.affiliation: Anses Niort Laboratory, French Agency for Food, Environmental and Occupational Health & Safety, F-79024 Niort, France.
authors.forename: Carine
authors.initials: C
authors.lastname: Paraud
authors.affiliation: Anses Niort Laboratory, French Agency for Food, Environmental and Occupational Health & Safety, F-79024 Niort, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Pors
authors.affiliation: LUNAM Université, Oniris, Nantes-Atlantic College of Veterinary Medicine and Food Sciences and Engineering, F-44307 Nantes, France; INRA, UMR1300 Biology, Epidemiology and Risk Analysis in Animal Health, F-44307 Nantes, France. Electronic address: christophe.chartier@oniris-nantes.fr.
authors.forename: Christophe
authors.initials: C
authors.lastname: Chartier
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000469:parasitology*; D003457:Cryptosporidiosis / Q000188:drug therapy / Q000401:mortality / Q000662:veterinary; D016785:Cryptosporidium parvum; D005243:Feces / Q000469:parasitology; D015511:Goat Diseases / Q000188:drug therapy* / Q000401:mortality; D006041:Goats; D010270:Parasite Egg Count / Q000662:veterinary; D052999:Quinazolinones / Q000627:therapeutic use*; D016896:Treatment Outcome; D010880:Piperidines
chemical_list: D052999:Quinazolinones; C010176:halofuginone; D010880:Piperidines
keywords: Control methods-protozoa; Cryptosporidium parvum; Goat-protozoa; Halofuginone lactate