title: Delivery of green tea catechin and epigallocatechin gallate in liposomes incorporated into low-fat hard cheese.
abstract: The encapsulation of green tea catechin and epigallocatechin gallate (EGCG) in soy lecithin liposomes was examined at four concentrations (0%, 0.125%, 0.25% and 0.5% w/v), and inclusion in cheese at 0% and 0.25% w/v. The empty capsules had a mean diameter of 133nm and significantly (p<0.05) increased with the addition of catechin or EGCG. Electron microscopy revealed the lamellae and central core of the liposomes. Addition of antioxidants gave a significant (p<0.05) increase in the size of liposomes. Liposomes had surface potentials of -42.4 to -46.1mV with no significant difference between treatments, suggesting stable liposome systems. High efficiency (>70%) and yield (∼80%) were achieved from the incorporation of catechin or EGCG inside the liposome structure. Addition of either antioxidant increased the liposome phase transition temperature (>50°C). Nanocapsules containing these antioxidants were effectively retained within a low-fat hard cheese, presenting a simple and effective delivery vesicle for antioxidants.
authors.affiliation: Department of Food Science, University of Otago, PO Box 56, Dunedin 9054, New Zealand; Riddet Institute, Private Bag 11 222, Palmerston North 4442, New Zealand. Electronic address: ali.rashidinejad@otago.ac.nz.
authors.forename: Ali
authors.initials: A
authors.lastname: Rashidinejad
authors.affiliation: Department of Food Science, University of Otago, PO Box 56, Dunedin 9054, New Zealand.
authors.forename: E John
authors.initials: EJ
authors.lastname: Birch
authors.affiliation: Plant and Food Research, Auckland Mail Centre, Private Bag 92169, Auckland 1142, New Zealand.
authors.forename: Dongxiao
authors.initials: D
authors.lastname: Sun-Waterhouse
authors.affiliation: Department of Food Science, University of Otago, PO Box 56, Dunedin 9054, New Zealand; Riddet Institute, Private Bag 11 222, Palmerston North 4442, New Zealand.
authors.forename: David W
authors.initials: DW
authors.lastname: Everett
mesh_terms: D000975:Antioxidants; D002392:Catechin / Q000031:analogs & derivatives* / Q000737:chemistry*; D002611:Cheese / Q000032:analysis*; D008081:Liposomes / Q000737:chemistry*; D053769:Nanocapsules; D013662:Tea / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D008081:Liposomes; D053769:Nanocapsules; D013662:Tea; D002392:Catechin; C045651:epigallocatechin gallate
keywords: Antioxidants; Catechin; Cheese; Epigallocatechin gallate; Green tea; Liposome encapsulation