title: [Distribution of genotypes in ESBLs producing E. coli strains isolated from posthepatitic cirrhosis' patients with bloodstream infection].
abstract: OBJECTIVE
  To study the genotype distribution of extended-spectrum beta-lactamases (ESBLs) in ESBLs-producing Escherichia coli (E. coli) isolates from posthepatitic cirrhosis' patients with bloodstream infection.
  
  
  METHODS
  E. coli were isolated in bloodstream from patients with posthepatitic cirrhosis between January and December in 2011. The strains were identified by VITEK-II. The antibiol susceptibility tests were performed with K-B method. beta-lactamases genes were detected multi-PCR, PCR, sequence and blast.
  
  
  RESULTS
  A total of 79 non-duplicate clinical isolates of E coli were consecutively collected from liver cirrhosis' patients with bloodstream infection. There were 20 isolates produced TEM-1 type beta-lactamases and 1 isolate produced SHV-1 typebeta-lactamases. 40 clinical isolates were detected to produce CTX-M type ESBLs, there were 20 CTX-M-1 group and 26 CTX-M-9 group, including 6 stains habouring both CTX-M-1 and CTX-M-9 group. Eight CTX-M genotypes were confirmed by sequencing of the PCR products, including CTX-M-3, CTX-M-14, CTX-M-15, CTX-M-24, CTX-M-28, CTX-M-31, CTX-M-65 and CTX-M-79.
  
  
  CONCLUSIONS
  CTX-M genotype ESBLs was the most popular extended-spectrum beta-lactamases in E. coli isolated from liver cirrhosis' patients with bloodstream infection. The CTX-M-14 is the dominant epidemic type.
authors.forename: Tong-Sheng
authors.initials: TS
authors.lastname: Guo
authors.forename: En-Bo
authors.initials: EB
authors.lastname: Cui
authors.forename: Chun-Mei
authors.initials: CM
authors.lastname: Bao
authors.forename: Ju-Ling
authors.initials: JL
authors.lastname: Zhang
authors.forename: Fen
authors.initials: F
authors.lastname: Qu
authors.forename: Yuan-Li
authors.initials: YL
authors.lastname: Mao
authors.forename: Yu-Long
authors.initials: YL
authors.lastname: Cong
mesh_terms: D016470:Bacteremia / Q000382:microbiology*; D003428:Cross Infection / Q000382:microbiology*; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000382:microbiology*; D029968:Escherichia coli Proteins / Q000235:genetics*; D005838:Genotype; D006760:Hospitalization / Q000706:statistics & numerical data; D006801:Humans; D008103:Liver Cirrhosis / Q000628:therapy*; D008826:Microbial Sensitivity Tests; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism
chemical_list: D029968:Escherichia coli Proteins; D001618:beta-Lactamases