title: The combination of intralymphatic chemotherapy with ultrasound and nano-/microbubbles is efficient in the treatment of experimental tumors in mouse lymph nodes.
abstract: Intravenous chemotherapy is a therapeutic option for the treatment of lymph node metastasis, but the drugs often have difficulty accessing the lymphatic system. The aim of this study was to determine whether the combination of intralymphatic chemotherapy with ultrasound and nano-/microbubbles is active against tumors in mouse lymph nodes. Intralymphatic chemotherapy in mice with lymph nodes containing tumors was found to have a marked anti-tumor effect, compared with intravenous administration, and the addition of ultrasound combined with nano-/microbubbles enhanced the effect of the anti-cancer drug, but only when the drug was administered intralymphatically. Furthermore, decreases in the volumes and blood vessel densities of tumor-bearing lymph nodes are reliable measures of therapeutic effect, confirmed by histopathological evaluation. The main conclusion is that combining ultrasound with nano-/microbubbles and intralymphatic chemotherapy improves drug delivery to the lymphatic system and has a more potent anti-tumor effect.
authors.affiliation: Graduate School of Biomedical Engineering, Tohoku University, Aoba, Sendai, Miyagi, Japan; Department of Urology, Tohoku University Graduate School of Medicine, Aoba, Sendai, Miyagi, Japan.
authors.forename: Takuma
authors.initials: T
authors.lastname: Sato
authors.affiliation: Department of Oral and Maxillofacial Surgery, Tohoku University Hospital, Aoba, Sendai, Miyagi, Japan.
authors.forename: Shiro
authors.initials: S
authors.lastname: Mori
authors.affiliation: Department of Urology, Tohoku University Graduate School of Medicine, Aoba, Sendai, Miyagi, Japan.
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Arai
authors.affiliation: Graduate School of Biomedical Engineering, Tohoku University, Aoba, Sendai, Miyagi, Japan. Electronic address: kodama@bme.tohoku.ac.jp.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Kodama
mesh_terms: D000818:Animals; D002945:Cisplatin / Q000008:administration & dosage / Q000494:pharmacology*; D003131:Combined Modality Therapy; D005290:Ferric Compounds / Q000627:therapeutic use*; D007091:Image Processing, Computer-Assisted; D015552:Injections, Intralesional; D007501:Iron / Q000627:therapeutic use*; D008081:Liposomes / Q000627:therapeutic use; D008207:Lymphatic Metastasis; D008223:Lymphoma / Q000188:drug therapy / Q000628:therapy*; D051379:Mice; D045423:Microbubbles / Q000627:therapeutic use*; D010087:Oxides / Q000627:therapeutic use*; D014464:Ultrasonic Therapy / Q000379:methods*
chemical_list: D005290:Ferric Compounds; D008081:Liposomes; D010087:Oxides; C069727:Sonazoid; D007501:Iron; D002945:Cisplatin
keywords: Intralymphatic chemotherapy; Lymph node metastasis; Sonoporation; Ultrasound