title: A cucumber DELLA homolog CsGAIP may inhibit staminate development through transcriptional repression of B class floral homeotic genes.
abstract: In hermaphroditic Arabidopsis, the phytohormone gibberellin (GA) stimulates stamen development by opposing the DELLA repression of B and C classes of floral homeotic genes. GA can promote male flower formation in cucumber (Cucumis sativus L.), a typical monoecious vegetable with unisexual flowers, and the molecular mechanism remains unknown. Here we characterized a DELLA homolog CsGAIP in cucumber, and we found that CsGAIP is highly expressed in stem and male flower buds. In situ hybridization showed that CsGAIP is greatly enriched in the stamen primordia, especially during the hermaphrodite stage of flower development. Further, CsGAIP protein is located in nucleus. CsGAIP can partially rescue the plant height, stamen development and fertility phenotypes of Arabidopsis rga-24/gai-t6 mutant, and ectopic expression of CsGAIP in wide-type Arabidopsis results in reduced number of stamens and decreased transcription of B class floral homeotic genes APETALA3 (AP3) and PISTILLATA (PI). Our data suggest that monoecious CsGAIP may inhibit staminate development through transcriptional repression of B class floral homeotic genes in Arabidopsis.
authors.affiliation: Department of Vegetable Science, College of Agronomy and Bio-technology, China Agricultural University, Beijing, P.R. China; Department of Vegetable Science, Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, China Agricultural University, Beijing, P.R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Vegetable Science, College of Agronomy and Bio-technology, China Agricultural University, Beijing, P.R. China; Department of Vegetable Science, Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, China Agricultural University, Beijing, P.R. China.
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.affiliation: Department of Vegetable Science, College of Agronomy and Bio-technology, China Agricultural University, Beijing, P.R. China; Department of Vegetable Science, Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, China Agricultural University, Beijing, P.R. China.
authors.forename: Sen
authors.initials: S
authors.lastname: Yang
authors.affiliation: Department of Vegetable Science, College of Agronomy and Bio-technology, China Agricultural University, Beijing, P.R. China; Department of Vegetable Science, Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, China Agricultural University, Beijing, P.R. China.
authors.forename: Jingbo
authors.initials: J
authors.lastname: An
authors.affiliation: Department of Vegetable Science, College of Agronomy and Bio-technology, China Agricultural University, Beijing, P.R. China; Department of Vegetable Science, Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, China Agricultural University, Beijing, P.R. China.
authors.forename: Chunhua
authors.initials: C
authors.lastname: Chen
authors.affiliation: Department of Vegetable Science, College of Agronomy and Bio-technology, China Agricultural University, Beijing, P.R. China; Department of Vegetable Science, Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, China Agricultural University, Beijing, P.R. China.
authors.forename: Xiaolan
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Vegetable Science, College of Agronomy and Bio-technology, China Agricultural University, Beijing, P.R. China; Department of Vegetable Science, Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, China Agricultural University, Beijing, P.R. China.
authors.forename: Huazhong
authors.initials: H
authors.lastname: Ren
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics; D018553:Cucumis sativus / Q000235:genetics* / Q000378:metabolism*; D035264:Flowers / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D005816:Genetic Complementation Test; D005875:Gibberellins / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D058974:Phylogeography; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism*; D021381:Protein Transport
chemical_list: D029681:Arabidopsis Proteins; D005875:Gibberellins; D010940:Plant Proteins