title: Regulation of zinc transporter 3 and carbonic anhydrases 2 and 14 mRNA expression in the retina of rats affected by low dietary zinc.
abstract: We looked at how zinc transporter 3 ZnT-3) mRNA expression in the rat retina is affected by low dietary zinc. Groups of 12 four-week-old male Sprague Dawley rats were fed on a low-zinc diet for 2, 4 or 6 weeks. Half of each group was then fed with a normal-zinc content diet and the other half was given a low-zinc content diet. The expression of ZnT-3, carbonic anhydrase 2 (CA2) and 14 (CA14) were detected by RT-PCR. After the rats were fed a low-zinc content diet for 2 weeks, their retina CA2 and CA14 mRNA levels were decreased, and the ZnT-3 mRNA was increased compared with the control rats. After they were fed a low-zinc diet for 4 weeks, ZnT-3, CA2 and CA14 mRNA levels decreased significantly. Then, after being changed back to a normal diet for 2 weeks, the rats had ZnT-3, CA2 and CA14 mRNA levels recovery in the retina.
authors.affiliation: First Affiliated Hospital of Nanjing Medical University, Maternal and Child Health Hospital of Jiangsu Province, Nanjing, Jiangsu Province, China peibinzhangcn@163.com.
authors.forename: P B
authors.initials: PB
authors.lastname: Zhang
authors.affiliation: Maternal and Child Health Hospital of Changzhou, Nanjing, Jiangsu Province, China.
authors.forename: H Y
authors.initials: HY
authors.lastname: Xiao
authors.affiliation: First Affiliated Hospital of Nanjing Medical University, Maternal and Child Health Hospital of Jiangsu Province, Nanjing, Jiangsu Province, China.
authors.forename: H
authors.initials: H
authors.lastname: Liu
authors.affiliation: First Affiliated Hospital of Nanjing Medical University, Maternal and Child Health Hospital of Jiangsu Province, Nanjing, Jiangsu Province, China.
authors.forename: R
authors.initials: R
authors.lastname: Qin
mesh_terms: D000818:Animals; D002256:Carbonic Anhydrases / Q000235:genetics*; D027682:Cation Transport Proteins / Q000235:genetics*; D004032:Diet; D005786:Gene Expression Regulation / Q000187:drug effects; D008297:Male; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D012160:Retina / Q000378:metabolism*; D014801:Vitamin A / Q000097:blood; D015032:Zinc / Q000008:administration & dosage* / Q000097:blood / Q000494:pharmacology
chemical_list: D027682:Cation Transport Proteins; D012333:RNA, Messenger; C503697:zinc transporter 3, rat; D014801:Vitamin A; D002256:Carbonic Anhydrases; D015032:Zinc