title: Calmodulin modulates insect odorant receptor function.
abstract: Insect odorant receptors (ORs) are heteromeric complexes of an odor-specific receptor protein (OrX) and a ubiquitous co-receptor protein (Orco). The ORs operate as non-selective cation channels, also conducting Ca(2+) ions. The Orco protein contains a conserved putative calmodulin (CaM)-binding motif indicating a role of CaM in its function. Using Ca(2+) imaging to monitor OR activity we investigated the effect of CaM inhibition on the function of OR proteins. Ca(2+) responses elicited in Drosophila olfactory sensory neurons by stimulation with the synthetic OR agonist VUAA1 were reduced and prolonged by CaM inhibition with the potent antagonist W7 but not with the weak antagonist W5. A similar effect was observed for Orco proteins heterologously expressed in CHO cells when CaM was inhibited with W7, trifluoperazine or chlorpromazine, or upon overexpression of CaM-EF-hand mutants. With the Orco CaM mutant bearing a point mutation in the putative CaM site (K339N) the Ca(2+) responses were akin to those obtained for wild type Orco in the presence of W7. There was no uniform effect of W7 on Ca(2+) responses in CHO cells expressing complete ORs (Or22a/Orco, Or47a/Orco, Or33a/Orco, Or56a/Orco). For Or33a and Or47a we observed no significant effect of W7, while it caused a reduced response in cells expressing Or22a and a shortened response for Or56a.
authors.affiliation: Max Planck Institute for Chemical Ecology, Department Evolutionary Neuroethology, Hans-Knöll-St. 8, D-07745 Jena, Germany.
authors.forename: Latha
authors.initials: L
authors.lastname: Mukunda
authors.affiliation: Max Planck Institute for Chemical Ecology, Department Evolutionary Neuroethology, Hans-Knöll-St. 8, D-07745 Jena, Germany.
authors.forename: Fabio
authors.initials: F
authors.lastname: Miazzi
authors.affiliation: Max Planck Institute for Chemical Ecology, Department Evolutionary Neuroethology, Hans-Knöll-St. 8, D-07745 Jena, Germany.
authors.forename: Sabine
authors.initials: S
authors.lastname: Kaltofen
authors.affiliation: Max Planck Institute for Chemical Ecology, Department Evolutionary Neuroethology, Hans-Knöll-St. 8, D-07745 Jena, Germany.
authors.forename: Bill S
authors.initials: BS
authors.lastname: Hansson
authors.affiliation: Max Planck Institute for Chemical Ecology, Department Evolutionary Neuroethology, Hans-Knöll-St. 8, D-07745 Jena, Germany. Electronic address: dwicher@ice.mpg.de.
authors.forename: Dieter
authors.initials: D
authors.lastname: Wicher
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D016466:CHO Cells; D002118:Calcium / Q000378:metabolism*; D002147:Calmodulin / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D006224:Cricetinae; D003412:Cricetulus; D004330:Drosophila; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D018034:Olfactory Receptor Neurons / Q000187:drug effects / Q000378:metabolism; D018035:Receptors, Odorant / Q000819:agonists / Q000378:metabolism*; D016415:Sequence Alignment; D013449:Sulfonamides / Q000494:pharmacology; D013864:Thioglycolates / Q000494:pharmacology; D014230:Triazoles / Q000494:pharmacology
chemical_list: D002147:Calmodulin; C572612:N-(4-ethylphenyl)-2-((4-ethyl-5-(3-pyridinyl)-4H-1,2,4-triazol-3-yl)thio)acetamide; D018035:Receptors, Odorant; D013449:Sulfonamides; D013864:Thioglycolates; D014230:Triazoles; C017967:W 7; D002118:Calcium
keywords: Calcium imaging; Drosophila; Odorant receptor; Olfactory sensory neuron; Orco