title: The development and amino acid binding ability of nano-materials based on azo derivatives: theory and experiment.
abstract: Two nano-material-containing azo groups have been designed and developed, and the binding ability of nano-materials with various amino acids has been characterized by UV-vis and fluorescence titrations. Results indicated that two nano-materials showed the strongest binding ability for homocysteine among twenty normal kinds of amino acids (alanine, valine, leucine, isoleucine, methionine, aspartic acid, glutamic acid, arginine, glycine, serine, threonine, asparagine, phenylalanine, histidine, tryptophan, proline, lysine, glutamine, tyrosine and homocysteine). The reason for the high sensitivity for homocysteine was that two nano-materials containing an aldehyde group reacted with SH in homocysteine and afforded very stable thiazolidine derivatives. Theoretical investigation further illustrated the possible binding mode in host-guest interaction and the roles of molecular frontier orbitals in molecular interplay. Thus, the two nano-materials can be used as optical sensors for the detection of homocysteine.
authors.affiliation: Department of Chemistry, Xinxiang Medical University, Jinsui Road 601, Xinxiang, Henan 453003, China. Electronic address: xuefangshang@126.com.
authors.forename: Xuefang
authors.initials: X
authors.lastname: Shang
authors.affiliation: Sanquan Medical College, Xinxiang Medical University, East of Xiangyang Road, Xinxiang, Henan 453003, China.
authors.forename: Jinge
authors.initials: J
authors.lastname: Du
authors.affiliation: Department of Pathology, Xinxiang Medical University, Jinsui Road 601, Xinxiang, Henan 453003, China.
authors.forename: Wancai
authors.initials: W
authors.lastname: Yang
authors.affiliation: School of Pharmacy, Xinxiang Medical University, Jinsui Road 601, Xinxiang, Henan 453003, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Liu
authors.affiliation: School of Pharmacy, Xinxiang Medical University, Jinsui Road 601, Xinxiang, Henan 453003, China.
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Fu
authors.affiliation: School of Pharmacy, Xinxiang Medical University, Jinsui Road 601, Xinxiang, Henan 453003, China.
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Wei
authors.affiliation: Analysis and Testing Laboratory, Xinxiang Medical University, Jinsui Road 601, Xinxiang, Henan 453003, China.
authors.forename: Ruifang
authors.initials: R
authors.lastname: Yan
authors.affiliation: Analysis and Testing Laboratory, Xinxiang Medical University, Jinsui Road 601, Xinxiang, Henan 453003, China.
authors.forename: Ningcong
authors.initials: N
authors.lastname: Yao
authors.affiliation: Sanquan Medical College, Xinxiang Medical University, East of Xiangyang Road, Xinxiang, Henan 453003, China.
authors.forename: Yaping
authors.initials: Y
authors.lastname: Guo
authors.affiliation: School of Pharmacy, Xinxiang Medical University, Jinsui Road 601, Xinxiang, Henan 453003, China.
authors.forename: Jinlian
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Chemistry, Nankai University, Tianjin 300071, China.
authors.forename: Xiufang
authors.initials: X
authors.lastname: Xu
mesh_terms: D000596:Amino Acids / Q000737:chemistry / Q000378:metabolism*; D001391:Azo Compounds / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D008958:Models, Molecular; D008968:Molecular Conformation; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000596:Amino Acids; D001391:Azo Compounds
keywords: Azo derivative; Homocysteine detection; Nano-material; Theoretical investigation