title: Intestinal proteomics in pig models of necrotising enterocolitis, short bowel syndrome and intrauterine growth restriction.
abstract: Necrotising enterocolitis (NEC), short bowel syndrome (SBS) and intrauterine growth restriction (IUGR) are three conditions associated with intestinal dysfunction in newborn infants, particularly those born preterm. Piglet (Sus scrofa) models have recently been developed for NEC, SBS and IUGR, and tissue proteomic analyses have identified unknown pathways and new prognostic disease markers. Intestinal HSPs, iron metabolism proteins and proteins related to amino acid (e.g. arginine) and glucose metabolism are consistently affected by NEC progression and some of these proteins are also affected by SBS and IUGR. Parallel changes in some plasma and urinary proteins (e.g. haptoglobin, globulins, complement proteins, fatty acid binding proteins) may mirror the intestinal responses and pave the way to biomarker discovery. Explorative non-targeted proteomics provides ideas about the cellular pathways involved in intestinal adaptation during the critical neonatal period. Proteomics, combined with other -omic techniques, helps to get a more holistic picture of intestinal adaptation during NEC, SBS and IUGR. Explorative -omic research methods also have limitations and cannot replace, but only supplement, classical hypothesis-driven research that investigate disease mechanisms using a single or few endpoints.
authors.affiliation: Department of Nutrition, Exercise and Sports, University of Copenhagen, Frederiksberg, Denmark.
authors.forename: Pingping
authors.initials: P
authors.lastname: Jiang
authors.forename: Per Torp
authors.initials: PT
authors.lastname: Sangild
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D020345:Enterocolitis, Necrotizing / Q000378:metabolism*; D005317:Fetal Growth Retardation / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism*; D040901:Proteomics; D012778:Short Bowel Syndrome / Q000378:metabolism*; D013552:Swine
keywords: Birth; IUGR; Intestine; Milk; NEC; Newborn; Pig model; SBS