title: Determination of moxifloxacin and its oxidation products with kinetic evaluation under potassium permanganate treatment in acidic solution by ultra-performance liquid chromatography/tandem mass spectrometry.
abstract: A simple, sensitive, and reproducible ultra-performance LC method for the determination of moxifloxacin (MOXI) oxidation stability under permanganate treatment in acidic conditions (pH 3.0-6.0) was developed. Besides the MOXI peak [retention time (RT) = 2.58], four additional products (RT = 0.86, 0.91, 1.42, and 1.89) were observed in all conditions tested. The oxidation process followed second-order reaction kinetics and depended upon solution acidity. The highest reaction rate constant was observed at pH 3.0, and this value decreased as the pH was increased to 6.0. The oxidation products were characterized, and their fragmentation pathways, derived from MS/MS data, were proposed. Two of these products were identified as hydroxyl derivatives of MOXI and two others as their oxidation product analogs with molecular ions of 418.4 and 416.4 m/z, respectively.
authors.forename: Urszula
authors.initials: U
authors.lastname: Hubicka
authors.forename: Paweł
authors.initials: P
authors.lastname: Zmudzki
authors.forename: Paweł
authors.initials: P
authors.lastname: Zajdel
authors.forename: Jan
authors.initials: J
authors.lastname: Krzek
mesh_terms: D000042:Absorption; D001372:Aza Compounds / Q000032:analysis* / Q000737:chemistry; D002626:Chemistry, Pharmaceutical / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002853:Chromatography, Liquid / Q000379:methods*; D024841:Fluoroquinolones; D006863:Hydrogen-Ion Concentration; D007477:Ions; D007700:Kinetics; D000077266:Moxifloxacin; D010084:Oxidation-Reduction; D010100:Oxygen / Q000737:chemistry*; D011196:Potassium Permanganate / Q000737:chemistry*; D011804:Quinolines / Q000032:analysis* / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013997:Time Factors
chemical_list: D001372:Aza Compounds; D024841:Fluoroquinolones; D007477:Ions; D011804:Quinolines; D011196:Potassium Permanganate; D010100:Oxygen; D000077266:Moxifloxacin