title: Inhibitory effect of collagen-derived tripeptides on dipeptidylpeptidase-IV activity.
abstract: The collagen tripeptide fragments Gly-Ala-Hyp, Gly-Pro-Ala and Gly-Pro-Hyp were generated by hydrolyzing collagen from pig-skin, cattle-skin, fish-scales and chicken-feet, respectively, with Streptomyces collagenase. Collagenase treatment increased the concentration of tripeptides in the hydrolysates by 13-15% (w/w). Of the three peptides, Gly-Pro-Hyp was a true peptidic inhibitor of dipeptidylpeptidase-IV (DPP-IV), because DPP-IV could not hydrolyze the bond between Pro-Hyp. This tripeptide was a moderately competitive inhibitor (Ki=4.5 mM) of DPP-IV, and its level in the collagen hydrolysates could be greatly increased (4-9% [w/w]) using Streptomyces collagenase.
authors.affiliation: Research Institute for Biological Sciences (RIBS), Okayama Prefectural Technology Center for Agriculture, Forestry, and Fisheries, Kaga-Gun , Okayama , Japan.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Hatanaka
authors.forename: Kayoko
authors.initials: K
authors.lastname: Kawakami
authors.forename: Misugi
authors.initials: M
authors.lastname: Uraji
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification; D002417:Cattle; D002645:Chickens; D003094:Collagen / Q000737:chemistry* / Q000302:isolation & purification; D017364:Collagenases / Q000737:chemistry / Q000302:isolation & purification; D018819:Dipeptidyl Peptidase 4 / Q000737:chemistry*; D057075:Enzyme Assays; D005399:Fishes; D006868:Hydrolysis; D007700:Kinetics; D009842:Oligopeptides / Q000737:chemistry* / Q000302:isolation & purification; D011480:Protease Inhibitors / Q000737:chemistry* / Q000302:isolation & purification; D013302:Streptomyces / Q000737:chemistry / Q000201:enzymology; D013552:Swine
chemical_list: D001426:Bacterial Proteins; D009842:Oligopeptides; D011480:Protease Inhibitors; D003094:Collagen; D018819:Dipeptidyl Peptidase 4; D017364:Collagenases
keywords: Collagenase; DPP-IV; collagen hydrolysate; diabetes