title: The influences of parental diet and vitamin E intake on the embryonic zebrafish transcriptome.
abstract: The composition of the typical commercial diet fed to zebrafish can dramatically vary. By utilizing defined diets we sought to answer two questions: 1) How does the embryonic zebrafish transcriptome change when the parental adults are fed a commercial lab diet compared with a sufficient, defined diet (E+)? 2) Does a vitamin E-deficient parental diet (E-) further change the embryonic transcriptome? We conducted a global gene expression study using embryos from zebrafish fed a commercial (Lab), an E+ or an E- diet. To capture differentially expressed transcripts prior to onset of overt malformations observed in E- embryos at 48h post-fertilization (hpf), embryos were collected from each group at 36hpf. Lab embryos differentially expressed (p<0.01) 946 transcripts compared with the E+ embryos, and 2656 transcripts compared with the E- embryos. The differences in protein, fat and micronutrient intakes in zebrafish fed the Lab compared with the E+ diet demonstrate that despite overt morphologic consistency, significant differences in gene expression occurred. Moreover, functional analysis of the significant transcripts in the E- embryos suggested perturbed energy metabolism, leading to overt malformations and mortality. Thus, these findings demonstrate that parental zebrafish diet has a direct impact on the embryonic transcriptome.
authors.affiliation: Linus Pauling Institute, Oregon State University, Corvallis, OR 97331, USA; Molecular and Cellular Biology Program, Oregon State University, Corvallis, OR 97331, USA.
authors.forename: Galen W
authors.initials: GW
authors.lastname: Miller
authors.affiliation: Department of Environmental and Molecular Toxicology, Oregon State University, Corvallis, OR 97331, USA.
authors.forename: Lisa
authors.initials: L
authors.lastname: Truong
authors.affiliation: Environmental Health Sciences Center, Oregon State University, Corvallis, OR 97331, USA.
authors.forename: Carrie L
authors.initials: CL
authors.lastname: Barton
authors.affiliation: Linus Pauling Institute, Oregon State University, Corvallis, OR 97331, USA.
authors.forename: Edwin M
authors.initials: EM
authors.lastname: Labut
authors.affiliation: Linus Pauling Institute, Oregon State University, Corvallis, OR 97331, USA; School of Biological and Population Health Sciences, Oregon State University, Corvallis, OR 97331, USA.
authors.forename: Katie M
authors.initials: KM
authors.lastname: Lebold
authors.affiliation: Linus Pauling Institute, Oregon State University, Corvallis, OR 97331, USA; Environmental Health Sciences Center, Oregon State University, Corvallis, OR 97331, USA; School of Biological and Population Health Sciences, Oregon State University, Corvallis, OR 97331, USA.
authors.forename: Maret G
authors.initials: MG
authors.lastname: Traber
authors.affiliation: Department of Environmental and Molecular Toxicology, Oregon State University, Corvallis, OR 97331, USA; Environmental Health Sciences Center, Oregon State University, Corvallis, OR 97331, USA. Electronic address: robert.tanguay@oregonstate.edu.
authors.forename: Robert L
authors.initials: RL
authors.lastname: Tanguay
mesh_terms: D000818:Animals; D004032:Diet; D004625:Embryo, Nonmammalian; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D008297:Male; D059467:Transcriptome / Q000187:drug effects / Q000235:genetics*; D014810:Vitamin E / Q000494:pharmacology*; D015027:Zebrafish / Q000196:embryology*
chemical_list: D014810:Vitamin E
keywords: Defined diet; Microarray; Transcriptome; Vitamin E; Zebrafish