title: The relationships of phytosterols and oxyphytosterols in plasma and aortic valve cusps in patients with severe aortic stenosis.
abstract: Phytosterols such as campesterol and sitosterol are susceptible to oxidation by reactive oxygen species. We hypothesize that the plant sterols (PS) campesterol and sitosterol and their 7-oxygenated metabolites (POPs) correlate within and between human plasma and aortic valve cusps tissues. Plasma and tissue concentrations of PS and POPs were analyzed by gas chromatography-mass spectrometry-selected ion monitoring. Prior to analysis valve cusps tissue was mechanically separated from the calcified parts. PS and POP levels per dry cusps tissue weight were significantly higher compared with the concentrations in the calcified part. Against our hypothesis we found that despite the fact that there is a high correlation between plant sterols in and between plasma and valves cusps tissue, as well as a high correlation between plant sterols and oxyphytosterols and oxyphytosterols themselves within the valve cusps tissue, there was hardly any correlation in the amount of oxyphytosterols in plasma and between plasma and valves. Because plasma samples are easily accessible for large scale population based studies, we have to understand in more detail what the analysis of POPs implies in terms of CVD risk for the future.
authors.affiliation: Institute for Clinical Chemistry and Clinical Pharmacology, University Clinics Bonn, Sigmund-Freud-Str. 25, D-53105 Bonn, Germany.
authors.forename: Hans-Frieder
authors.initials: HF
authors.lastname: Schött
authors.affiliation: Abteilung für Kardiologie, Angiologie und internistische Intensivmedizin, Klinik für Innere Medizin III, Universitätsklinikum des Saarlandes, Homburg/Saar, Germany.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Luister
authors.affiliation: Institute for Clinical Chemistry and Clinical Pharmacology, University Clinics Bonn, Sigmund-Freud-Str. 25, D-53105 Bonn, Germany.
authors.forename: Constanze
authors.initials: C
authors.lastname: Husche
authors.affiliation: Abteilung für Thorax-und Herz-und Gefäßchirurgie, Universitätsklinikum des Saarlandes, Germany.
authors.forename: Hans-Joachim
authors.initials: HJ
authors.lastname: Schäfers
authors.affiliation: Abteilung für Kardiologie, Angiologie und internistische Intensivmedizin, Klinik für Innere Medizin III, Universitätsklinikum des Saarlandes, Homburg/Saar, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Böhm
authors.affiliation: Department of Human Biology, NUTRIM, School for Nutrition, Toxicology and Metabolism, Maastricht University Medical Center, PO Box 616, NL-6200 MD Maastricht, The Netherlands.
authors.forename: Jogchum
authors.initials: J
authors.lastname: Plat
authors.affiliation: Institute for Clinical Chemistry and Clinical Pharmacology, University Clinics Bonn, Sigmund-Freud-Str. 25, D-53105 Bonn, Germany. Electronic address: dieter.luetjohann@ukb.uni-bonn.de.
authors.forename: Dieter
authors.initials: D
authors.lastname: Lütjohann
authors.affiliation: Abteilung für Kardiologie, Angiologie und internistische Intensivmedizin, Klinik für Innere Medizin III, Universitätsklinikum des Saarlandes, Homburg/Saar, Germany.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Laufs
authors.affiliation: Abteilung für Kardiologie, Angiologie und internistische Intensivmedizin, Klinik für Innere Medizin III, Universitätsklinikum des Saarlandes, Homburg/Saar, Germany; Abteilung für Kardiologie, Klinikum Oldenburg, European-Medical School Oldenburg-Groningen, Oldenburg, Germany.
authors.forename: Oliver
authors.initials: O
authors.lastname: Weingärtner
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001021:Aortic Valve / Q000378:metabolism* / Q000601:surgery; D001024:Aortic Valve Stenosis / Q000097:blood*; D002784:Cholesterol / Q000031:analogs & derivatives / Q000097:blood / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010840:Phytosterols / Q000097:blood* / Q000378:metabolism*; D012855:Sitosterols / Q000097:blood / Q000378:metabolism
chemical_list: C116880:7-ketositosterol; D010840:Phytosterols; D012855:Sitosterols; C021273:campesterol; C025473:gamma-sitosterol; D002784:Cholesterol
keywords: Atherosclerosis; Atherosclerotic plaque; Oxyphytosterols; Oxysterols; Plant sterols