title: Correlations of programmed death 1 expression and serum IL-6 level with exhaustion of cytomegalovirus-specific T cells after allogeneic hematopoietic stem cell transplantation.
abstract: The effect of programmed death 1 (PD-1) on cytomegalovirus (CMV)-specific T cells has not been thoroughly examined. We evaluated the involvement of exhausted CMV-specific T cells in persistent CMV infection after allogeneic hematopoietic stem cell transplantation (HSCT). CMV-specific CD8+ T cells obtained from an HLA-A∗24:02-positive patient, who failed to eliminate CMV for more than 1 year after HSCT, responded poorly to CMV pp65 peptide and showed high PD-1 expression. Sera from patients with persistent CMV infection showed a significantly higher IL-6 level than those from patients with temporary CMV infection after allogeneic HSCT and healthy donors. CD33+ adherent cells produced IL-6, and regulated PD-1 expression and growth of CMV-specific CD8+ T cells through cell-to-cell contact. Although further investigation is required to clarify the association between IL-6 level and CMV infection in more patients, IL-6 might be a useful biomarker of persistent CMV infection after allogeneic HSCT.
authors.affiliation: Department of Hematology and Oncology, Nagoya University Graduate School of Medicine, Japan.
authors.forename: Tomonori
authors.initials: T
authors.lastname: Kato
authors.affiliation: Department of Hematology and Oncology, Nagoya University Graduate School of Medicine, Japan. Electronic address: tnishida@med.nagoya-u.ac.jp.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Nishida
authors.affiliation: Department of Pediatrics, Nagoya University Graduate School of Medicine, Japan.
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Ito
authors.affiliation: Department of Hematology and Oncology, Nagoya University Graduate School of Medicine, Japan.
authors.forename: Miho
authors.initials: M
authors.lastname: Murase
authors.affiliation: Department of Hematology and Oncology, Nagoya University Graduate School of Medicine, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Murata
authors.affiliation: Department of Hematology and Oncology, Nagoya University Graduate School of Medicine, Japan.
authors.forename: Tomoki
authors.initials: T
authors.lastname: Naoe
mesh_terms: D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000473:pathology; D002448:Cell Adhesion; D002450:Cell Communication; D003587:Cytomegalovirus / Q000276:immunology*; D003586:Cytomegalovirus Infections / Q000235:genetics* / Q000276:immunology / Q000473:pathology / Q000821:virology; D015870:Gene Expression; D059871:HLA-A24 Antigen / Q000235:genetics / Q000276:immunology; D018380:Hematopoietic Stem Cell Transplantation; D054884:Host-Pathogen Interactions; D006801:Humans; D007108:Immune Tolerance; D015850:Interleukin-6 / Q000235:genetics* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D061026:Programmed Cell Death 1 Receptor / Q000235:genetics* / Q000276:immunology; D063268:Sialic Acid Binding Ig-like Lectin 3 / Q000235:genetics / Q000276:immunology; D015398:Signal Transduction; D014184:Transplantation, Homologous; D019562:Viral Load
chemical_list: C571163:CD33 protein, human; C438506:HLA-A*24:02 antigen; D059871:HLA-A24 Antigen; C508600:IL6 protein, human; D015850:Interleukin-6; C105992:PDCD1 protein, human; D061026:Programmed Cell Death 1 Receptor; D063268:Sialic Acid Binding Ig-like Lectin 3
keywords: Allogeneic hematopoietic stem cell transplantation; Cytomegalovirus-specific T cell; Exhaustion; IL-6; Programmed death 1