title: Purification of O2-sensitive metalloproteins.
abstract: The most dependable factor to perform successful biochemical experiments in an O2-free environment is the experience required to set up an efficient laboratory, to properly manipulate samples, to anticipate potential O2-related problems, and to maintain the complex laboratory setup operative. There is a long list of O2-related issues that may ruin your experiments. We provide here a guide to minimize these risks.
authors.affiliation: Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid, Campus de Montegancedo, Pozuelo de, Alarcón, Madrid, 28223, Spain.
authors.forename: Carlos
authors.initials: C
authors.lastname: Echavarri-Erasun
authors.forename: Simon
authors.initials: S
authors.lastname: Arragain
authors.forename: Luis M
authors.initials: LM
authors.lastname: Rubio
mesh_terms: D000693:Anaerobiosis / Q000187:drug effects; D001671:Biochemistry / Q000379:methods*; D002021:Buffers; D002851:Chromatography, High Pressure Liquid; D004227:Dithionite / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000494:pharmacology; D008667:Metalloproteins / Q000302:isolation & purification* / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism*; D019163:Reducing Agents / Q000494:pharmacology
chemical_list: D002021:Buffers; D016166:Free Radical Scavengers; D008667:Metalloproteins; D019163:Reducing Agents; D004227:Dithionite; D010100:Oxygen