title: Reappraisal of quantitative gel zymography for matrix metalloproteinases.
abstract: BACKGROUND
  The determination of matrix metalloproteases (MMPs) is relevant in many pathophysiological conditions, especially if associated with extracellular matrix remodeling; however, the results obtained are closely linked to the method used and are not directly comparable. The aim of this study was to perform a reappraisal of quantitative gel zymography technique for MMPs in human plasma, to use for comparison with commercially available ELISA and in those experimental conditions where the MMP active form needs to be revealed.
  
  
  METHODS
  The critical methodological parameters of zymography were checked and a comparison with a routinely used ELISA was performed.
  
  
  RESULTS
  Sensitivity and reproducibility levels of zymography are suitable for detection of MMP-9 in human plasma, providing results closely related to those obtained by ELISA.
  
  
  CONCLUSIONS
  Analytical parameters of zymography were suitable for detection of MMPs in human plasma. Quantitative zymography for MMPs is an alternative method for comparing the results of ELISA widely employed for MMP determination, thus reducing the discrepancies between laboratories regarding gelatinase assay.
authors.affiliation: Consiglio Nazionale delle Ricerche (CNR), Institute of Clinical Physiology, Laboratory of Cardiovascular Biochemistry, Pisa, Italy.
authors.forename: Tommaso
authors.initials: T
authors.lastname: Prescimone
authors.forename: Danika
authors.initials: D
authors.lastname: Tognotti
authors.forename: Chiara
authors.initials: C
authors.lastname: Caselli
authors.forename: Manuela
authors.initials: M
authors.lastname: Cabiati
authors.forename: Andrea
authors.initials: A
authors.lastname: D'Amico
authors.forename: Silvia
authors.initials: S
authors.lastname: Del Ry
authors.forename: Daniela
authors.initials: D
authors.lastname: Giannessi
mesh_terms: D001774:Blood Chemical Analysis / Q000592:standards; D004591:Electrophoresis, Polyacrylamide Gel / Q000592:standards*; D057075:Enzyme Assays / Q000592:standards*; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards; D005260:Female; D006801:Humans; D016015:Logistic Models; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000097:blood*; D020782:Matrix Metalloproteinases / Q000097:blood; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D020782:Matrix Metalloproteinases; D020780:Matrix Metalloproteinase 9
keywords: ELISA; analytical performance; gel zymography; metalloproteases