title: Omega-3 fatty acids protect renal functions by increasing docosahexaenoic acid-derived metabolite levels in SHR.Cg-Lepr(cp)/NDmcr rats, a metabolic syndrome model.
abstract: The omega-3 polyunsaturated fatty acids (ω-3 PUFAs) docosahexaenoic acid (DHA) and/or eicosapentaenoic acid (EPA) protect against diabetic nephropathy by inhibiting inflammation. The aim of this study was to assess the effects of highly purified DHA and EPA or EPA only administration on renal function and renal eicosanoid and docosanoid levels in an animal model of metabolic syndrome, SHR.Cg-Lepr(cp)/NDmcr (SHRcp) rats. Male SHRcp rats were divided into 3 groups. Control (5% arabic gum), TAK-085 (300 mg/kg/day, containing 467 mg/g EPA and 365 mg/g DHA), or EPA (300 mg/kg/day) was orally administered for 20 weeks. The urinary albumin to creatinine ratio in the TAK-085-administered group was significantly lower than that in other groups. The glomerular sclerosis score in the TAK-085-administered group was significantly lower than that in the other groups. Although DHA levels were increased in total kidney fatty acids, the levels of nonesterified DHA were not significantly different among the 3 groups, whereas the levels of protectin D1, resolvin D1, and resolvin D2 were significantly increased in the TAK-085-administered group. The results show that the use of combination therapy with DHA and EPA in SHRcp rats improved or prevented renal failure associate with metabolic syndrome with decreasing triglyceride levels and increasing ω-3 PUFA lipid mediators.
authors.affiliation: Department of Environmental Physiology, Shimane University Faculty of Medicine, Izumo, Shimane 693-8501, Japan. katakura@med.shimane-u.ac.jp.
authors.forename: Masanori
authors.initials: M
authors.lastname: Katakura
authors.affiliation: Department of Environmental Physiology, Shimane University Faculty of Medicine, Izumo, Shimane 693-8501, Japan. michio1@med.shimane-u.ac.jp.
authors.forename: Michio
authors.initials: M
authors.lastname: Hashimoto
authors.affiliation: Department of Environmental Physiology, Shimane University Faculty of Medicine, Izumo, Shimane 693-8501, Japan. tinoue@med.shimane-u.ac.jp.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Inoue
authors.affiliation: Department of Environmental Physiology, Shimane University Faculty of Medicine, Izumo, Shimane 693-8501, Japan. mamun104@gmail.com.
authors.forename: Abdullah
authors.initials: A
authors.lastname: Al Mamun
authors.affiliation: Department of Environmental Physiology, Shimane University Faculty of Medicine, Izumo, Shimane 693-8501, Japan. tanabey@med.shimane-u.ac.jp.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Tanabe
authors.affiliation: Department of Health Chemistry, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan. riwamoto@mol.f.u-tokyo.ac.jp.
authors.forename: Ryo
authors.initials: R
authors.lastname: Iwamoto
authors.affiliation: Department of Health Chemistry, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan. marita@mol.f.u-tokyo.ac.jp.
authors.forename: Makoto
authors.initials: M
authors.lastname: Arita
authors.affiliation: Disease Model Cooperative Research Association, Hamamatsu, Shizuoka, 433-8114, Japan. shrtsuchikura@h5.dion.ne.jp.
authors.forename: Satoru
authors.initials: S
authors.lastname: Tsuchikura
authors.affiliation: Department of Environmental Physiology, Shimane University Faculty of Medicine, Izumo, Shimane 693-8501, Japan. o-shido@med.shimane-u.ac.jp.
authors.forename: Osamu
authors.initials: O
authors.lastname: Shido
mesh_terms: D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D004195:Disease Models, Animal; D004281:Docosahexaenoic Acids / Q000031:analogs & derivatives / Q000378:metabolism*; D015777:Eicosanoids / Q000378:metabolism; D015525:Fatty Acids, Omega-3 / Q000737:chemistry / Q000494:pharmacology*; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism* / Q000473:pathology; D007677:Kidney Function Tests; D050356:Lipid Metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D024821:Metabolic Syndrome / Q000378:metabolism*; D051381:Rats; D011918:Rats, Inbred SHR
chemical_list: D015777:Eicosanoids; D015525:Fatty Acids, Omega-3; D004281:Docosahexaenoic Acids