title: Olive oil and omega-3 polyunsaturated fatty acids suppress intestinal polyp growth by modulating the apoptotic process in ApcMin/+ mice.
abstract: The promotion and progression of carcinogenesis are susceptible to nutritional interventions aimed at counteracting cancer development. Lipid metabolism is essential in the onset and progression of tumors and for cancer cell survival. In this study, we tested the effects of diets enriched with natural compounds, such as olive oil and salmon oil, in mice that spontaneously develop intestinal polyps (Apc(Min/+) mice). For this purpose, we evaluated polyp number and volume, intestinal mucosa proliferation/apoptosis, estrogen receptors (ERs) expression, fatty acid synthase and 3-hydroxy-3-methylglutaryl coenzyme A (HMGCoA) reductase gene expression and enzymatic activity. Compared with the standard diet, the salmon oil-enriched diet, containing a high percentage of omega-3 polyunsaturated fatty acids, and, to a lesser extent, olive oil-enriched diet reduced polyp number and volume through a reduction of proliferation and a marked proapoptotic effect. These biological effects were mediated by an inhibition of fatty acid synthase and HMGCoA reductase gene expression and activity and an increase of ERβ/ERα ratio. Our findings suggest that a proper dietary lifestyle could contribute to primary cancer prevention.
authors.affiliation: Gastroenterology Unit, Department of Medical and Surgical Sciences, University of Foggia, Foggia 71122, Italy, Laboratory of Biochemistry, National Institute for Digestive Diseases 'S. de Bellis', Castellana Grotte, Bari 70013, Italy and Gastroenterology Unit, Department of Emergency and Organ Transplantation (D.E.T.O.), University of Bari, Bari 70124, Italy.
authors.forename: Michele
authors.initials: M
authors.lastname: Barone
authors.affiliation: Laboratory of Biochemistry, National Institute for Digestive Diseases 'S. de Bellis', Castellana Grotte, Bari 70013, Italy and.
authors.forename: Maria
authors.initials: M
authors.lastname: Notarnicola
authors.affiliation: Laboratory of Biochemistry, National Institute for Digestive Diseases 'S. de Bellis', Castellana Grotte, Bari 70013, Italy and.
authors.forename: Maria G
authors.initials: MG
authors.lastname: Caruso
authors.affiliation: Gastroenterology Unit, Department of Emergency and Organ Transplantation (D.E.T.O.), University of Bari, Bari 70124, Italy.
authors.forename: Maria P
authors.initials: MP
authors.lastname: Scavo
authors.affiliation: Gastroenterology Unit, Department of Emergency and Organ Transplantation (D.E.T.O.), University of Bari, Bari 70124, Italy.
authors.forename: Maria T
authors.initials: MT
authors.lastname: Viggiani
authors.affiliation: Laboratory of Biochemistry, National Institute for Digestive Diseases 'S. de Bellis', Castellana Grotte, Bari 70013, Italy and.
authors.forename: Valeria
authors.initials: V
authors.lastname: Tutino
authors.affiliation: Laboratory of Biochemistry, National Institute for Digestive Diseases 'S. de Bellis', Castellana Grotte, Bari 70013, Italy and Gastroenterology Unit, Department of Emergency and Organ Transplantation (D.E.T.O.), University of Bari, Bari 70124, Italy.
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Polimeno
authors.affiliation: Laboratory of Biochemistry, National Institute for Digestive Diseases 'S. de Bellis', Castellana Grotte, Bari 70013, Italy and.
authors.forename: Barbara
authors.initials: B
authors.lastname: Pesetti
authors.affiliation: Gastroenterology Unit, Department of Emergency and Organ Transplantation (D.E.T.O.), University of Bari, Bari 70124, Italy.
authors.forename: Alfredo
authors.initials: A
authors.lastname: Di Leo
authors.affiliation: Laboratory of Biochemistry, National Institute for Digestive Diseases 'S. de Bellis', Castellana Grotte, Bari 70013, Italy and direzionescientifica@irccsdebellis.it.
authors.forename: Antonio
authors.initials: A
authors.lastname: Francavilla
mesh_terms: D025601:Adenomatous Polyposis Coli Protein / Q000502:physiology*; D000818:Animals; D017209:Apoptosis; D015153:Blotting, Western; D049109:Cell Proliferation; D003110:Colonic Neoplasms / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D015525:Fatty Acids, Omega-3 / Q000627:therapeutic use*; D006903:Hydroxymethylglutaryl CoA Reductases / Q000235:genetics / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism; D007417:Intestinal Polyps / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D050356:Lipid Metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008861:Microsomes / Q000201:enzymology; D000069463:Olive Oil; D010938:Plant Oils / Q000008:administration & dosage*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019014:fas Receptor / Q000235:genetics / Q000378:metabolism
chemical_list: D025601:Adenomatous Polyposis Coli Protein; C491899:Fas protein, mouse; D015525:Fatty Acids, Omega-3; D000069463:Olive Oil; D010938:Plant Oils; D012333:RNA, Messenger; D019014:fas Receptor; D006903:Hydroxymethylglutaryl CoA Reductases