title: The influences of pH control strategies on the distribution of 1,3-propanediols and 2,3-butanediols production by an isolated indigenous Klebsiella sp. Ana-WS5.
abstract: In the simultaneous biological production of 1,3-propanediols (PDO) and 2,3-butanediols (BDO), glycerol was suggested to be a suitable carbon source. Extra addition of 10 g/L of lactic acid can create about a 30% increase in total diols production compared to the control batch. Several different pH control strategies were investigated. The results indicated that the batch with the uncontrolled pH had the highest total diols production among all pH control strategies, although it had the lowest productivity. Even the strategy of pH fluctuation did not enhance total diols production, it significantly enhanced the productivity. The soluble metabolite products (SMPs) analysis also indicated that the pH fluctuation will only affect BDO production, but had no impacts on the induction of more metabolites produced. Conclusively, both adding lactic acid and the pH fluctuation strategy are simple and efficient methods of simultaneously enhancing BDO and PDO production.
authors.affiliation: Department of Chemical and Materials Engineering, Tunghai University, Taichung 407, Taiwan. Electronic address: hwyen@thu.edu.tw.
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Yen
authors.affiliation: Department of Chemical and Materials Engineering, Tunghai University, Taichung 407, Taiwan.
authors.forename: Fang-Tzu
authors.initials: FT
authors.lastname: Li
authors.affiliation: Department of Chemical Engineering, National Cheng Kung University, Tainan 701, Taiwan; Research Center for Energy Technology and Strategy, National Cheng Kung University, Tainan 701, Taiwan; University Center for Bioscience and Biotechnology, National Cheng Kung University, Tainan 701, Taiwan.
authors.forename: Jo-Shu
authors.initials: JS
authors.lastname: Chang
mesh_terms: D019149:Bioreactors / Q000382:microbiology; D001709:Biotechnology / Q000379:methods*; D002072:Butylene Glycols / Q000378:metabolism*; D002244:Carbon / Q000494:pharmacology; D005285:Fermentation / Q000187:drug effects; D005947:Glucose / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007709:Klebsiella / Q000187:drug effects / Q000302:isolation & purification* / Q000378:metabolism*; D019344:Lactic Acid / Q000494:pharmacology; D011409:Propylene Glycols / Q000378:metabolism*; D012995:Solubility
chemical_list: D002072:Butylene Glycols; D011409:Propylene Glycols; D019344:Lactic Acid; C026978:2,3-butylene glycol; C041787:1,3-propanediol; D002244:Carbon; D005947:Glucose; D005990:Glycerol
keywords: Butanediols; Klebsiella; Lactic acid; Propanediols; pH adjustment