title: A novel system for cloning human TCRs: Cutting short the way to TCR-based anticancer therapy.
abstract: T-cell receptor (TCR)-based gene immunotherapy has emerged as a promising approach for the treatment of multiple malignancies. We have recently reported an efficient system for the cloning and functional evaluation of TCR-coding cDNAs. This system, which we named hTEC10, allows for the determination of TCR antigen specificity in less than 10 days, and may therefore constitute a fast and powerful platform for the development of new TCR-based anticancer therapies.
authors.affiliation: Department of Immunology; Graduate School of Medicine and Pharmaceutical Sciences; University of Toyama; Toyama, Japan.
authors.forename: Eiji
authors.initials: E
authors.lastname: Kobayashi
authors.affiliation: Department of Immunology; Graduate School of Medicine and Pharmaceutical Sciences; University of Toyama; Toyama, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kishi
authors.affiliation: Department of Immunology; Graduate School of Medicine and Pharmaceutical Sciences; University of Toyama; Toyama, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Muraguchi
keywords: T-cell receptor; gene therapy; hTEC10; single-cell RT-PCR