title: Rhizobium flavum sp. nov., a triazophos-degrading bacterium isolated from soil under the long-term application of triazophos.
abstract: A Gram-stain-negative, non-motile, pale yellow, rod-shaped bacterial strain, YW14(T), was isolated from soil and its taxonomic position was investigated by a polyphasic study. Strain YW14(T) did not form nodules on three different legumes, and the nodD and nifH genes were not detected by PCR. Strain YW14(T) contained Q-10 as the predominant ubiquinone. The major cellular fatty acid was C(18 : 1)ω7c. Phylogenetic analyses based on 16S rRNA gene sequences and seven housekeeping gene sequences (recA, atpD, glnII, gyrB, rpoB, dnaK and thrC) showed that strain YW14(T) belonged to the genus Rhizobium. Strain YW14(T) showed 16S rRNA gene sequence similarity of 93.4-97.3% to the type strains of recognized species of the genus Rhizobium. DNA-DNA relatedness between strain YW14(T) and the type strains of Rhizobium sullae IS123(T) and Rhizobium yanglingense CCBAU 71623(T) was 19.6-25.7%, indicating that strain YW14(T) was distinct from them genetically. Strain YW14(T) could also be differentiated from these phylogenetically related species of the genus Rhizobium by various phenotypic properties. On the basis of phenotypic properties, phylogenetic distinctiveness and genetic data, strain YW14(T) is considered to represent a novel species of the genus Rhizobium, for which the name Rhizobium flavum sp. nov. is proposed. The type strain is YW14(T) ( = KACC 17222(T) = CCTCC AB2013042(T)).
authors.affiliation: College of life science, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Tao
authors.initials: T
authors.lastname: Gu
authors.affiliation: College of life science, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Li Na
authors.initials: LN
authors.lastname: Sun
authors.affiliation: Jiangsu Provincial Coordinated Research Center for Organic Solid Waste Utilization, Nanjing Agricultural University, Nanjing 210095, PR China.|Key Laboratory of Plant Nutrition and Fertilization in Low-Middle Reaches of the Yangtze River, Ministry of Agriculture, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Agro-Microbial Resource and Application, Ministry of Agriculture/College of Biological Sciences, China Agricultural University, Beijing 100094, PR China.
authors.forename: Xin Hua
authors.initials: XH
authors.lastname: Sui
authors.affiliation: College of life science, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Shun Peng
authors.initials: SP
authors.lastname: Li
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D007887:Fabaceae / Q000382:microbiology; D005227:Fatty Acids / Q000737:chemistry; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D063086:Organothiophosphates / Q000378:metabolism*; D010802:Phylogeny; D010858:Pigmentation; D055170:Plant Root Nodulation; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012231:Rhizobium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D012989:Soil Pollutants / Q000378:metabolism; D014230:Triazoles / Q000378:metabolism*; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D063086:Organothiophosphates; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; D014230:Triazoles; D014451:Ubiquinone; C012584:triazophos; C025204:Ubiquinone Q2