title: Synthesis of multifunctional Fe₃O₄-CdSe/ZnS nanoclusters coated with lipid A toward dendritic cell-based immunotherapy.
abstract: We demonstrate a novel route to synthesize Fe3O4-CdSe/ZnS multifunctional nanoclusters (MNCs) with excellent optical and magnetic properties and biocompatibility. The successful fabrication of highly fluorescent and magnetic MNCs is achieved via a coupling process based on a partial ligand exchange reaction at the aqueous-organic solution interface. In addition, we show that dendritic cells (DCs), the sentinel of the immune system, can uptake the MNCs without significant change in cell viability. The MNCs uptaken by the DCs can be used for imaging, tracking, and separating the DCs. Furthermore, the MNCs can be loaded with a pathogen-associated molecular pattern, lipid A, via a hydrophobic-hydrophobic interaction. Ex vivo labeling of DCs with the MNC-lipid A complex enhances the DC migration to draining lymph nodes and tumor antigen-specific T cell responses in vivo. Our work may contribute to the development of synthetic routes to various multifunctional nanoclusters and DC-based cancer immunotherapies.
authors.affiliation: Department of Materials Science and Engineering, Korea University , Seoul 136-713, Republic of Korea.
authors.forename: Jinhoo
authors.initials: J
authors.lastname: Jeong
authors.forename: Eun-Kyung
authors.initials: EK
authors.lastname: Kwon
authors.forename: Taek-Chin
authors.initials: TC
authors.lastname: Cheong
authors.forename: Heeyeon
authors.initials: H
authors.lastname: Park
authors.forename: Nam-Hyuk
authors.initials: NH
authors.lastname: Cho
authors.forename: Woong
authors.initials: W
authors.lastname: Kim
mesh_terms: D002465:Cell Movement; D003713:Dendritic Cells / Q000737:chemistry* / Q000166:cytology / Q000276:immunology; D006801:Humans; D007167:Immunotherapy / Q000295:instrumentation* / Q000379:methods; D008050:Lipid A / Q000737:chemistry*; D008198:Lymph Nodes / Q000276:immunology; D008280:Magnetics; D049329:Nanostructures / Q000737:chemistry*; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D008050:Lipid A; D011108:Polymers