title: Exposure to inhomogeneous static magnetic field beneficially affects allergic inflammation in a murine model.
abstract: Previous observations suggest that static magnetic field (SMF)-exposure acts on living organisms partly through reactive oxygen species (ROS) reactions. In this study, we aimed to define the impact of SMF-exposure on ragweed pollen extract (RWPE)-induced allergic inflammation closely associated with oxidative stress. Inhomogeneous SMF was generated with an apparatus validated previously providing a peak-to-peak magnetic induction of the dominant SMF component 389 mT by 39 T m(-1) lateral gradient in the in vivo and in vitro experiments, and 192 mT by 19 T m(-1) in the human study at the 3 mm target distance. Effects of SMF-exposure were studied in a murine model of allergic inflammation and also in human provoked skin allergy. We found that even a single 30-min exposure of mice to SMF immediately following intranasal RWPE challenge significantly lowered the increase in the total antioxidant capacity of the airways and decreased allergic inflammation. Repeated (on 3 consecutive days) or prolonged (60 min) exposure to SMF after RWPE challenge decreased the severity of allergic responses more efficiently than a single 30-min treatment. SMF-exposure did not alter ROS production by RWPE under cell-free conditions, while diminished RWPE-induced increase in the ROS levels in A549 epithelial cells. Results of the human skin prick tests indicated that SMF-exposure had no significant direct effect on provoked mast cell degranulation. The observed beneficial effects of SMF are likely owing to the mobilization of cellular ROS-eliminating mechanisms rather than direct modulation of ROS production by pollen NAD(P)H oxidases.
authors.affiliation: Department of Immunology, Faculty of Medicine, University of Debrecen, , 98 Nagyerdei Boulevard, Debrecen 4012, Hungary.
authors.forename: Anikó
authors.initials: A
authors.lastname: Csillag
authors.forename: Brahma V
authors.initials: BV
authors.lastname: Kumar
authors.forename: Krisztina
authors.initials: K
authors.lastname: Szabó
authors.forename: Mária
authors.initials: M
authors.lastname: Szilasi
authors.forename: Zsuzsa
authors.initials: Z
authors.lastname: Papp
authors.forename: Magdolna E
authors.initials: ME
authors.lastname: Szilasi
authors.forename: Kitti
authors.initials: K
authors.lastname: Pázmándi
authors.forename: István
authors.initials: I
authors.lastname: Boldogh
authors.forename: Éva
authors.initials: É
authors.lastname: Rajnavölgyi
authors.forename: Attila
authors.initials: A
authors.lastname: Bácsi
authors.forename: János F
authors.initials: JF
authors.lastname: László
mesh_terms: D000328:Adult; D000818:Animals; D001249:Asthma / Q000378:metabolism / Q000473:pathology / Q000628:therapy; D002460:Cell Line; D003876:Dermatitis, Atopic / Q000378:metabolism / Q000473:pathology / Q000628:therapy; D004195:Disease Models, Animal; D004311:Double-Blind Method; D005260:Female; D006801:Humans; D007249:Inflammation / Q000378:metabolism / Q000473:pathology / Q000628:therapy; D055909:Magnetic Field Therapy / Q000295:instrumentation / Q000379:methods*; D060526:Magnetic Fields; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008875:Middle Aged; D017382:Reactive Oxygen Species; D006255:Rhinitis, Allergic, Seasonal / Q000378:metabolism / Q000473:pathology / Q000628:therapy
chemical_list: D017382:Reactive Oxygen Species
keywords: allergic inflammation; oxidative stress; pollen; static magnetic field