title: TiO2-graphene nanocomposites for enhanced osteocalcin induction.
abstract: Bone defects and damages are common these days, which increases the usage of biomaterial for humans. To prepare a potential biomaterial, we synthesised a series of titania-graphene nanocomposites (TGS) (2:x (0.25, 0.5, 1.0, 2.0, and 4.0 g)) using in situ sol-gel method. The obtained structural results show that the prepared TGS nanocomposites are an irregular sheet with spherical TiO2 intercalated morphology. The SSA of the nanocomposites ranging from 167.98 to 234.56 m(2) g(-1) with mesoporosity and swelling tendency ranging from 11.55 to 26.13% leads to an enhancement in human cell attachment as well as avoids the migration and agglomeration of the nanoparticles in the body. Further, the biological analysis in simulated body fluid and human cell lines (AGS and MG-63) collectively reveals that the TG2 (2:2) and TG4 (2:4) samples are found to be more favourable materials for biomimic bone action among the prepared TGS nanocomposites.
authors.affiliation: Centre for Nano Science and Technology, K.S. Rangasamy College of Technology, Tiruchengode 637 215, Tamil Nadu, India.
authors.forename: Kavitha
authors.initials: K
authors.lastname: Kandiah
authors.affiliation: Centre for Nano Science and Technology, K.S. Rangasamy College of Technology, Tiruchengode 637 215, Tamil Nadu, India.
authors.forename: Prabhu
authors.initials: P
authors.lastname: Muthusamy
authors.affiliation: Centre for Nano Science and Technology, K.S. Rangasamy College of Technology, Tiruchengode 637 215, Tamil Nadu, India.
authors.forename: Selvam
authors.initials: S
authors.lastname: Mohan
authors.affiliation: Centre for Nano Science and Technology, K.S. Rangasamy College of Technology, Tiruchengode 637 215, Tamil Nadu, India. Electronic address: veerajendran@gmail.com.
authors.forename: Rajendran
authors.initials: R
authors.lastname: Venkatachalam
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology; D001672:Biocompatible Materials / Q000494:pharmacology; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D006108:Graphite / Q000494:pharmacology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007477:Ions; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D015675:Osteocalcin / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared; D013059:Spectrum Analysis, Raman; D013696:Temperature; D014025:Titanium / Q000494:pharmacology*; D014961:X-Ray Diffraction
chemical_list: D000890:Anti-Infective Agents; D001672:Biocompatible Materials; D007477:Ions; D015675:Osteocalcin; C009495:titanium dioxide; D006108:Graphite; D014025:Titanium
keywords: Biocompatibility; Cytotoxicity; MG-63 cell line; Osteocalcin; Surface area; Titania–graphene