title: Simultaneous utilization of glucose and mannose from spent yeast cell mass for lipid production by Lipomyces starkeyi.
abstract: With ever-increasing culture of yeasts for the production of biofuels and other metabolites, spent yeast cell mass exceeds its traditional market demands. Yeast cell mass contains glucose, mannose and other sugars that may be utilized for microbial culture. Here we demonstrated that the oleaginous yeast Lipomyces starkeyi could utilize glucose and mannose simultaneously for lipid production. Overall substrate consumption rates and lipid coefficients were 0.58 g/L/h and 0.18 g lipid/g sugar, respectively, in flask cultures regardless of glucose, mannose or a mixture of both as the carbon source. L. starkeyi grew well on the hydrolysates of spent cell mass of Rhodosporidium toruloides, consumed both glucose and mannose therein, and produced lipid at a yield of 0.12 g lipid/g total reducing sugars. This co-utilization strategy expands carbon sources for lipid production. It should provide an opportunity for recycling spent cell mass and be of significant interests to biorefinery and biofuel production.
authors.affiliation: Dalian Institute of Chemical Physics, CAS, Dalian 116023, PR China; Dalian University of Technology, Dalian 116024, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Yang
authors.affiliation: Dalian Institute of Chemical Physics, CAS, Dalian 116023, PR China.
authors.forename: Guojie
authors.initials: G
authors.lastname: Jin
authors.affiliation: Dalian Institute of Chemical Physics, CAS, Dalian 116023, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Gong
authors.affiliation: Dalian Institute of Chemical Physics, CAS, Dalian 116023, PR China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Shen
authors.affiliation: Dalian Institute of Chemical Physics, CAS, Dalian 116023, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
authors.forename: Yehua
authors.initials: Y
authors.lastname: Song
authors.affiliation: Dalian University of Technology, Dalian 116024, PR China.
authors.forename: Fengwu
authors.initials: F
authors.lastname: Bai
authors.affiliation: Dalian Institute of Chemical Physics, CAS, Dalian 116023, PR China. Electronic address: zhaozb@dicp.ac.cn.
authors.forename: Zongbao K
authors.initials: ZK
authors.lastname: Zhao
mesh_terms: D005227:Fatty Acids / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D008055:Lipids / Q000096:biosynthesis*; D055160:Lipomyces / Q000378:metabolism*; D008358:Mannose / Q000378:metabolism*
chemical_list: D005227:Fatty Acids; D008055:Lipids; D005947:Glucose; D008358:Mannose
keywords: Lipomyces starkeyi; Mannose; Microbial lipids; Oleaginous yeast; Spent yeast cell mass