title: Influence of enzyme loading on enzymatic hydrolysis of cardboard waste and size distribution of the resulting fiber residue.
abstract: Enzymatic hydrolysis of lignocellulosic biomass to sugars alters the properties of the cellulosic fibers. Several process variables, including enzyme loading, play an important role in these changes. Many physical properties of fibers are affected: their length and width, porosity, specific surface area, and degree of fibrillation, for instance, may undergo dramatic changes when subjected to enzymatic degradation. In this study, the influence of enzyme loading on the fiber size was investigated using milled cardboard waste as the raw material. The effect of cellulases and hemicellulases on the monosaccharide production and the resulting fiber size was studied using commercial enzyme products. It was shown that the cellulase loading largely determined the amount of sugars produced. The fiber length was reduced during the course of hydrolysis, although the size reduction was not especially dramatic. Based on the SEM images, no significant damage to the fiber surfaces occurred during the process.
authors.affiliation: Laboratory of Separation Technology, LUT Chemistry, Lappeenranta University of Technology, P.O. Box 20, FI-53851 Lappeenranta, Finland. Electronic address: teemu.kinnarinen@lut.fi.
authors.forename: Teemu
authors.initials: T
authors.lastname: Kinnarinen
authors.affiliation: Laboratory of Separation Technology, LUT Chemistry, Lappeenranta University of Technology, P.O. Box 20, FI-53851 Lappeenranta, Finland.
authors.forename: Antti
authors.initials: A
authors.lastname: Häkkinen
mesh_terms: D002480:Cellulase / Q000378:metabolism*; D002482:Cellulose / Q000737:chemistry* / Q000378:metabolism* / Q000648:ultrastructure; D005947:Glucose / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006868:Hydrolysis; D008970:Molecular Weight; D014866:Waste Products
chemical_list: D014866:Waste Products; D002482:Cellulose; D006026:Glycoside Hydrolases; C023305:hemicellulase; D002480:Cellulase; D005947:Glucose
keywords: Cardboard; Cellulase; Enzyme loading; Fiber size; Hydrolysis