title: Case control study of the factor V Leiden and factor II G20210A mutation frequency in women with recurrent pregnancy loss.
abstract: BACKGROUND
  Recurrent pregnancy loss (RPL) caused by various genetic and non-genetic factors. After chromosome abnormality, thrombophilia is one of the most important genetic factors that could cause RPL. Factor V Leiden and factor II G20210A mutation were the most common mutations cause thrombophilia in the world.
  
  
  OBJECTIVE
  The purpose of this study was to determine the frequency of factor V Leiden and prothrombine gene mutations in women with RPL compared with women who had uneventful pregnancies.
  
  
  METHODS
  This case control study evaluates the frequency of factor V-Leiden and factor II G20210 genotypes in 80 women with two or more pregnancy losses, compared with 80 women without adverse pregnancy outcome. The mutations were assessed by PCR-RFLP.
  
  
  RESULTS
  Frequency of the factor V Leiden among cases was 2.5%, which was higher than controls (1.25%), but the difference was not significant. No factor II G20210 mutation was found among cases and controls.
  
  
  CONCLUSIONS
  These data did not confirm that factor V Leiden and factor II G20210 mutation might play a role in recurrent pregnancy loss in Iranian women.
authors.affiliation: Department of Immunology, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
authors.forename: Majid
authors.initials: M
authors.lastname: Teremmahi Ardestani
authors.affiliation: Department of Immunology, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
authors.forename: Hossein Hadi
authors.initials: HH
authors.lastname: Nodushan
authors.affiliation: Department of Obstetrics and Gynecology, Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
authors.forename: Abbas
authors.initials: A
authors.lastname: Aflatoonian
authors.affiliation: Department of Medical Genetics, Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
authors.forename: Nasrin
authors.initials: N
authors.lastname: Ghasemi
authors.affiliation: Department of Medical Genetics, Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
authors.forename: Mohammad Hasan
authors.initials: MH
authors.lastname: Sheikhha
keywords: Factor II G20210A Mutation; Factor V Leiden; Recurrent Abortion