title: Variant alleles of VEGF and risk of esophageal cancer and lymph node metastasis.
abstract: The variant alleles of the functional polymorphisms, VEGF -2578 C > A, -1498 T > C and +936 C > T, were not associated with risk of esophageal cancer and lymph node metastasis. Compared with the most common haplotype, C-2578T-1498C+936, the A-2578C-1498C+936 haplotype was associated with a borderline significantly increased risk of esophageal cancer. C-2578C-1498C+936 and A-2578T-1498T+936 haplotypes were associated with significantly increased risk of esophageal cancer. Vascular endothelial growth factor (VEGF) is a potent stimulator for angiogenesis. It has been implicated in the growth and metastasis of esophageal cancer. Three functional single nucleotide polymorphisms (SNPs) of VEGF (-2578 C > A, -1498 T > C and +936 C > T) are known to be related to VEGF expression. We conducted a case-control study to evaluate the effects of these three functional SNPs on the development of esophageal cancer and lymph node metastasis. A total of 497 cases and 380 controls were analyzed. Genotypes were determined by matrix assisted laser desorption/ionization time-of-flight mass spectrometry and direct sequence methods. The variant alleles of the functional polymorphisms VEGF -2578 C > A, -1498 T > C and +936 C > T SNPs were not associated with esophageal cancer risk. These VEGF genotypes were not associated with the risk of esophageal cancer after stratification. Furthermore, no association was observed between VEGF -2578 C > A, -1498 T > C and +936 C > T polymorphisms and lymph node metastasis. Compared with the most common haplotype C-2578T-1498C+936, the A-2578C-1498C+936 haplotype was associated with a borderline significantly increased risk of esophageal cancer. C-2578C-1498C+936 and A-2578T-1498T+936 haplotypes were associated with significantly increased risk of esophageal cancer. Variants in the functional polymorphisms of VEGF may not contribute to esophageal cancer and lymph node metastasis susceptibility. VEGF A-2578C-1498C+936, C-2578C-1498C+936 and A-2578T-1498T+936 haplotypes may be associated with increased risk of esophageal cancer. However, our results were obtained with a limited sample size and therefore this is a preliminary conclusion. Validation by a larger study with more diverse ethnic populations is needed.
authors.affiliation: Department of Cardiothorac Surgery, Affiliated People's Hospital of Jiangsu University , Zhenjiang , P.R. China and.
authors.forename: Haiyong
authors.initials: H
authors.lastname: Gu
authors.forename: Wanshan
authors.initials: W
authors.lastname: Qiu
authors.forename: Yijun
authors.initials: Y
authors.lastname: Shi
authors.forename: Suocheng
authors.initials: S
authors.lastname: Chen
authors.forename: Jun
authors.initials: J
authors.lastname: Yin
mesh_terms: D000483:Alleles; D016022:Case-Control Studies; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000473:pathology; D005260:Female; D006239:Haplotypes; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D012307:Risk Factors; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics*
chemical_list: D042461:Vascular Endothelial Growth Factor A