title: The potential anticancer activity of extracts derived from the roots ofScutellaria baicalensison human oral squamous cell carcinoma cells.
abstract: Various herb products derived from plants have potent biological effects including anticancer activity. In the present study, the antitumor activity of a herbal product derived from theScutellaria baicalensis (S. baicalensis)was examined, usingin vitroassays in a human oral squamous cell carcinoma (OSCC) cell line. Results showed thatS. baicalensisroot extract at the concentration of 100μg/ml inhibited monolayer- and anchorage-independent growth in human OSCC cell lines, while not affecting the adhering abilities of cells. This suggested that it did not alter the expression of any of the adhesion receptors that mediate cell-extracellular matrix (ECM) interactions. TheS. baicalensisroot extract demonstrated potent cytostatic and apoptotic effects due to the downregulation of the cyclin-dependent kinase 4 expression and its partner cyclin D1, resulting in G1 arrest and poly (ADP-ribose) polymerase (PARP) cleavage. Additionally, theS. baicalensisroot extract was found to have blocked vascular endothelial growth factor (VEGF)-induced migration and tube formation in human endothelial cells. Taken together, these results demonstrate that as a herbal product, theS. baicalensisroot extract is a potential inhibitor of tumori- and angiogenesis and may be valuable in the development of pharmaceutical medications for the treatment of oral squamous cell carcinoma.
authors.affiliation: Department of Oral and Maxillofacial Surgery, School of Dentistry, Showa University, Ota-ku, Tokyo 145-8515;
authors.forename: Daisuke
authors.initials: D
authors.lastname: Sato
authors.affiliation: Department of Oral and Maxillofacial Surgery, School of Dentistry, Showa University, Ota-ku, Tokyo 145-8515;
authors.forename: Seiji
authors.initials: S
authors.lastname: Kondo
authors.affiliation: Laboratory of Nutraceuticals and Functional Foods Science, Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, Minato-ku, Tokyo 108-8477, Japan.
authors.forename: Kazunaga
authors.initials: K
authors.lastname: Yazawa
authors.affiliation: Department of Oral and Maxillofacial Surgery, School of Dentistry, Showa University, Ota-ku, Tokyo 145-8515;
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Mukudai
authors.affiliation: Department of Oral and Maxillofacial Surgery, School of Dentistry, Showa University, Ota-ku, Tokyo 145-8515;
authors.forename: Chunnan
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Oral and Maxillofacial Surgery, School of Dentistry, Showa University, Ota-ku, Tokyo 145-8515;
authors.forename: Takaaki
authors.initials: T
authors.lastname: Kamatani
authors.affiliation: Department of Oral and Maxillofacial Surgery, School of Dentistry, Showa University, Ota-ku, Tokyo 145-8515;
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Katsuta
authors.affiliation: Department of Oral and Maxillofacial Surgery, School of Dentistry, Showa University, Ota-ku, Tokyo 145-8515;
authors.forename: Yasuto
authors.initials: Y
authors.lastname: Yoshihama
authors.affiliation: Department of Oral and Maxillofacial Surgery, School of Dentistry, Showa University, Ota-ku, Tokyo 145-8515;
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Shirota
authors.affiliation: Department of Oral and Maxillofacial Surgery, School of Dentistry, Showa University, Ota-ku, Tokyo 145-8515;
authors.forename: Satoru
authors.initials: S
authors.lastname: Shintani
keywords: angiogenesis; anticancer therapeutic agents; herbal product; human oral squamous cell carcinoma