title: Optimization of preparation of antioxidative peptides from pumpkin seeds using response surface method.
abstract: Protein isolates of pumpkin (Cucurbita pepo L) seeds were hydrolyzed by acid protease to prepare antioxidative peptides. The hydrolysis conditions were optimized through Box-Behnken experimental design combined with response surface method (RSM). The second-order model, developed for the DPPH radical scavenging activity of pumpkin seed hydrolysates, showed good fit with the experiment data with a high value of coefficient of determination (0.9918). The optimal hydrolysis conditions were determined as follows: hydrolyzing temperature 50°C, pH 2.5, enzyme amount 6000 U/g, substrate concentration 0.05 g/ml and hydrolyzing time 5 h. Under the above conditions, the scavenging activity of DPPH radical was as high as 92.82%.
authors.affiliation: College of Life Science, Shanxi University, Taiyuan, China.
authors.forename: Sanhong
authors.initials: S
authors.lastname: Fan
authors.affiliation: College of Life Science, Shanxi University, Taiyuan, China.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Hu
authors.affiliation: College of Life Science, Shanxi University, Taiyuan, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Li
authors.affiliation: College of Life Science, Shanxi University, Taiyuan, China.
authors.forename: Yanrong
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000704:Analysis of Variance; D000975:Antioxidants / Q000378:metabolism*; D001709:Biotechnology / Q000379:methods*; D001713:Biphenyl Compounds / Q000378:metabolism; D028464:Cucurbita / Q000201:enzymology / Q000378:metabolism*; D016166:Free Radical Scavengers / Q000494:pharmacology; D006868:Hydrolysis / Q000187:drug effects; D008954:Models, Biological; D010455:Peptides / Q000302:isolation & purification*; D010851:Picrates / Q000378:metabolism; D012044:Regression Analysis; D015203:Reproducibility of Results; D012639:Seeds / Q000201:enzymology / Q000378:metabolism*; D013379:Substrate Specificity / Q000187:drug effects; D013997:Time Factors
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D010455:Peptides; D010851:Picrates; C004931:1,1-diphenyl-2-picrylhydrazyl