title: The identification, typing, and antimicrobial susceptibility of Pseudomonas aeruginosa isolated from mink with hemorrhagic pneumonia.
abstract: The biological characteristics and molecular epidemiology of Pseudomonas aeruginosa associated with mink hemorrhagic pneumonia from Shandong province of eastern China were determined in this study. From 2010 to 2011, 30 mink P. aeruginosa isolates were identified from lung, fecal and feed samples of clinical cases and subjected to serotyping, antimicrobial susceptibility testing and pulsed-field gel electrophoresis (PFGE) using SpeI. The P. aeruginosa isolates belonged to four serotypes-21 of type G, four of type I, three of type M, one of type B, and one non-typable strain. The strains were divided into four large groups as determined by PFGE. Isolates from the group 2 were highly homologous and were obtained from the same region as an epidemic. All of the isolates were sensitive to piperacillin, piperacillin/tazobactam, ceftazidime, cefepime, imipenem, amikacin, gentamicin and tobramycin and resistant to ampicillin, cefuroxime and cefuroxime axetil. A high frequency of resistance was found to ampicillin/sulbactam, cefazolin, cefotetan, ceftriaxone, nitrofurantoin, and trimethoprim/sulfamethoxazole (96.7%). Resistance to ticarcillin/clavulanic acid, ciprofloxacin and levofloxacin was less common (13.3%). There was no relationship between antibiotic resistance and serotype distribution of the isolates. The epidemic serotype of P. aeruginosa from the mink hemorrhagic pneumonia in Shandong province was type G, which was a clone of commonly found in this province. These findings reveal the genetic similarities and antimicrobial susceptibility profiles of P. aeruginosa from clinical cases of mink hemorrhagic pneumonia and will facilitate the prevention and control of the disease in Shandong province of China.
authors.affiliation: Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan 250100, China; College of Life Sciences, Shandong University, Jinan 250100, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Qi
authors.affiliation: Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan 250100, China.
authors.forename: Lulu
authors.initials: L
authors.lastname: Li
authors.affiliation: Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan 250100, China; College of Life Sciences, Shandong University, Jinan 250100, China.
authors.forename: Yijun
authors.initials: Y
authors.lastname: Du
authors.affiliation: College of Agricultural Sciences, Liaocheng University, Liaocheng 252059, China.
authors.forename: Shourong
authors.initials: S
authors.lastname: Wang
authors.affiliation: Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan 250100, China; College of Life Sciences, Shandong University, Jinan 250100, China.
authors.forename: Jinwen
authors.initials: J
authors.lastname: Wang
authors.affiliation: Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan 250100, China.
authors.forename: Yanbo
authors.initials: Y
authors.lastname: Luo
authors.affiliation: National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Che
authors.affiliation: National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors.forename: Jinxing
authors.initials: J
authors.lastname: Lu
authors.affiliation: Jinan Municipal Center for Disease Control & Prevention, Jinan 250021, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
authors.affiliation: Jinan Municipal Center for Disease Control & Prevention, Jinan 250021, China.
authors.forename: Guangchun
authors.initials: G
authors.lastname: Hu
authors.affiliation: Jinan Military General Hospital, Jinan 250031, China.
authors.forename: Jixia
authors.initials: J
authors.lastname: Li
authors.affiliation: Jinan Military General Hospital, Jinan 250031, China.
authors.forename: Yanwen
authors.initials: Y
authors.lastname: Gong
authors.affiliation: College of Life Sciences, Shandong University, Jinan 250100, China; Shandong Provincial Center for Animal Disease Control and Prevention, Jinan 250022, China.
authors.forename: Guisheng
authors.initials: G
authors.lastname: Wang
authors.affiliation: College of Life Sciences, Shandong University, Jinan 250100, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Hu
authors.affiliation: College of Life Sciences, Shandong University, Jinan 250100, China.
authors.lastname: Shiganyan
authors.affiliation: Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan 250100, China; College of Life Sciences, Shandong University, Jinan 250100, China. Electronic address: 13853183199@163.com.
authors.forename: Yuqing
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000821:Animal Feed / Q000382:microbiology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002681:China; D004352:Drug Resistance, Microbial; D016521:Electrophoresis, Gel, Pulsed-Field; D005243:Feces / Q000382:microbiology; D008168:Lung / Q000382:microbiology; D008826:Microbial Sensitivity Tests; D008907:Mink / Q000382:microbiology*; D010802:Phylogeny; D011014:Pneumonia / Q000382:microbiology / Q000662:veterinary*; D011552:Pseudomonas Infections / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000145:classification / Q000187:drug effects / Q000235:genetics; D012703:Serotyping
chemical_list: D000900:Anti-Bacterial Agents
keywords: Antimicrobial susceptibility; Identification; Mink; Pseudomonas aeruginosa; Pulsed-field gel electrophoresis; Serotyping