title: An explanation for the difference in the percutaneous penetration behavior of tamsulosin induced by two different O-acylmenthol derivatives.
abstract: Using tamsulosin (TAL) as a model drug, the aim of this study was to investigate and compare the percutaneous permeation behavior of two menthol derivatives, 2-isopropyl-5-methylcyclohexyl heptanoate (M-HEP) and 2-isopropyl-5-methylcyclohexyl decanoate (M-DEC). In vitro transdermal permeation study was carried out using porcine skin. The residual amount of enhancers in the skin after permeation experiment was determined by gas chromatographic (GC) method. The penetration depths of fluorescein were visualized by two-photon confocal laser scanning microscopy (2P-LSM) after the skin being treated with different enhancers. Furthermore, changes in the stretching frequency of functional group of ceramide were investigated by using attenuated total reflectance Fourier transform infrared (ATR-FTIR) technique. After M-HEP addition, the cumulative amount of TAL permeated in 8 h (Q8) reached 20.57±0.54 μg/cm2 and the depth of fluorescein was 40 μm; the CH2 of ceramide symmetric stretching frequency was 4 cm−1 blue shifted. However, M-DEC has an opposite effect on TAL permeation compared with that of M-HEP. TAL is a crucial factor affecting permeation procedure, and microenvironment of lipid region determines promotion capability of the enhancers.
authors.affiliation: Department of Pharmaceutical Sciences, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, 110016Liaoning, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Shang
authors.forename: Dongmei
authors.initials: D
authors.lastname: Cun
authors.forename: Honglei
authors.initials: H
authors.lastname: Xi
authors.forename: Liang
authors.initials: L
authors.lastname: Fang
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D002518:Ceramides / Q000737:chemistry; D006537:Heptanoates / Q000737:chemistry*; D008610:Menthol / Q000737:chemistry*; D039821:Monoterpenes / Q000737:chemistry*; D010539:Permeability / Q000187:drug effects*; D012867:Skin / Q000378:metabolism*; D012869:Skin Absorption / Q000187:drug effects*; D013449:Sulfonamides / Q000737:chemistry* / Q000378:metabolism*; D013552:Swine; D000077409:Tamsulosin
chemical_list: C000591317:2-isopropyl-5-methylcyclohexyl heptanoate; D002518:Ceramides; D006537:Heptanoates; D039821:Monoterpenes; D013449:Sulfonamides; D008610:Menthol; D000077409:Tamsulosin