title: Purification and characterization of bacteriocin produced by oral Lactobacillus paracasei SD1.
abstract: The present study aimed to purify and characterize the antimicrobial protein from Lactobacillus paracasei SD1, which is a strain from the human oral cavity. Antimicrobial activity was obtained from purifying the culture supernatant of L. paracasei SD1. Purification of the active compound was achieved with ammonium sulfate precipitation followed by chloroform and gel filtration chromatography. As revealed by SDS-PAGE, the active fraction was homogeneous, showing a protein with an approximate molecular weight of 25,000 Da. It was confirmed as having a molecular mass of 24,028.2 Da by mass spectrometry. The antimicrobial compound, named "paracasin SD1", exhibited a broad spectrum against oral pathogens. Paracasin SD1 was stable in a pH range between 3.0 and 8.0 at 100 °C for 5 min, and showed resistance to α-amylase, catalase, lysozyme and whole saliva. However, its activity was lost after proteinase K and trypsin treatment. The results obtained suggest the possibility of using paracasin SD1 for application in prevention/treatment of oral diseases.
authors.affiliation: Common Oral Diseases and Epidemiology Research Center and the Department of Stomatology, Faculty of Dentistry, Prince of Songkla University, Hat-Yai 90112, Thailand.
authors.forename: P
authors.initials: P
authors.lastname: Wannun
authors.affiliation: Common Oral Diseases and Epidemiology Research Center and the Department of Preventive Dentistry, Faculty of Dentistry, Prince of Songkla University Hat-Yai 90112, Thailand.
authors.forename: S
authors.initials: S
authors.lastname: Piwat
authors.affiliation: Common Oral Diseases and Epidemiology Research Center and the Department of Stomatology, Faculty of Dentistry, Prince of Songkla University, Hat-Yai 90112, Thailand. Electronic address: rawee.t@psu.ac.th.
authors.forename: R
authors.initials: R
authors.lastname: Teanpaisan
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D001430:Bacteriocins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D011232:Chemical Precipitation; D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D019286:Endopeptidase K / Q000378:metabolism; D006090:Gram-Negative Bacteria / Q000187:drug effects* / Q000254:growth & development; D006094:Gram-Positive Bacteria / Q000187:drug effects* / Q000254:growth & development; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007778:Lactobacillus / Q000737:chemistry* / Q000302:isolation & purification; D013058:Mass Spectrometry; D008826:Microbial Sensitivity Tests; D008970:Molecular Weight; D009055:Mouth; D055550:Protein Stability; D059748:Proteolysis; D013696:Temperature
chemical_list: D001426:Bacterial Proteins; D001430:Bacteriocins; D019286:Endopeptidase K
keywords: Bacteriocin; Characterization; Lactobacillus paracasei; Oral diseases; Paracasin SD1; Purification