title: Bioinformatics analysis of the structure and function of NADPH-cytochrome p450 reductase ofPlasmodium vivax.
abstract: The structure of NADPH-cytochrome p450 reductase (CPR) ofPlasmodium falciparum(P. falciparumor Pf) has been determined using bioinformatics analysis. However, that ofPlasmodium vivax(P. vivaxor Pv) has not yet been determined. This study aimed to analyze the structure and function of PvCPR using bioinformatics analysis. The results demonstrated that PvCPR was an unstable and alkaline enzyme located in the cytoplasm of parasites with a signal peptide. It possessed seven types of signal sites and eight protein-protein binding sites, and had a tertiary structure resembling a forceps with a single wing, which differed from that of PfCPR. It also had nine linear B-cell epitopes and 10 antigenicity sites, which were not homologous with the amino acid sequence ofHomo sapiens(H. sapiensor Hs) CPR and six fragments that were similar to fragments of immune-related protein sequences fromH. sapiens. Therefore, the function of PvCPR may be different from that of PfCPR, and PvCPR may participate in the immune escape ofP. vivax.
authors.affiliation: School of Tropical and Laboratory Medicine, Hainan Medical College, Haikou 571199; ; Hainan Provincial Key Laboratory of Tropical Medicine, Hainan Medical College, Haikou 571199;
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Fan
authors.affiliation: Hubei Provincial Center for Disease Control and Prevention, Wuhan 430079;
authors.forename: Kaijie
authors.initials: K
authors.lastname: Li
authors.affiliation: Department of Parasitology, Medical College, Jinan University, Guangzhou 510623;
authors.forename: Lingmin
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Biochemistry and Molecular Biology, Medical School, Wuhan University, Wuhan 430072;
authors.forename: Fan
authors.initials: F
authors.lastname: Chen
authors.affiliation: School of Tropical and Laboratory Medicine, Hainan Medical College, Haikou 571199;
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wu
authors.affiliation: School of Tropical and Laboratory Medicine, Hainan Medical College, Haikou 571199;
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.affiliation: School of Tropical and Laboratory Medicine, Hainan Medical College, Haikou 571199;
authors.forename: Saifeng
authors.initials: S
authors.lastname: Zhong
authors.affiliation: School of Tropical and Laboratory Medicine, Hainan Medical College, Haikou 571199;
authors.forename: Guifen
authors.initials: G
authors.lastname: Lin
authors.affiliation: School of Nursing, Hainan Medical College, Haikou 571199, P.R. China.
authors.forename: Guogang
authors.initials: G
authors.lastname: Yan
keywords: NADPH-cytochrome p450 reductase; Plasmodium falciparum; Plasmodium vivax; antigenicity sites; bioinformatics; immune escape; linear B-cell epitope