title: Prediction of absorption coefficients by pulsed laser induced photoacoustic measurements.
abstract: In the current study, a pulsed laser induced photoacoustic spectroscopy setup was designed and developed, aiming its application in clinical diagnostics. The setup was optimized with carbon black samples in water and with various tryptophan concentrations at 281nm excitations. The sensitivity of the setup was estimated by determining minimum detectable concentration of tryptophan in water at the same excitation, and was found to be 0.035mM. The photoacoustic experiments were also performed with various tryptophan concentrations at 281nm excitation for predicting optical absorption coefficients in them and for comparing the outcomes with the spectrophotometrically-determined absorption coefficients for the same samples. Absorption coefficients for a few serum samples, obtained from some healthy female volunteers, were also determined through photoacoustic and spectrophotometric measurements at the same excitations, which showed good agreement between them, indicating its clinical implications.
authors.affiliation: Biophysics Unit, School of Life Sciences (SOLS), Manipal University, Manipal 576104, India.
authors.forename: Mallika
authors.initials: M
authors.lastname: Priya
authors.affiliation: Division of Radiobiology and Toxicology, SOLS, Manipal University, Manipal 576104, India.
authors.forename: B S
authors.initials: BS
authors.lastname: Satish Rao
authors.affiliation: Department of Surgical Oncology, Kasturba Medical College, Manipal, Manipal University, Manipal 576104, India.
authors.forename: Satadru
authors.initials: S
authors.lastname: Ray
authors.affiliation: Biophysics Unit, School of Life Sciences (SOLS), Manipal University, Manipal 576104, India. Electronic address: kkmahato@gmail.com.
authors.forename: K K
authors.initials: KK
authors.lastname: Mahato
mesh_terms: D005260:Female; D006801:Humans; D061088:Photoacoustic Techniques / Q000295:instrumentation* / Q000379:methods*; D013057:Spectrum Analysis / Q000295:instrumentation* / Q000379:methods*; D014364:Tryptophan / Q000032:analysis* / Q000097:blood*
chemical_list: D014364:Tryptophan
keywords: Absorption coefficient; PAS setup; Photoacoustic spectroscopy; Serum; Spectrophotometry; Tryptophan