title: Neurogenesis and vascularization of the damaged brain using a lactate-releasing biomimetic scaffold.
abstract: Regenerative medicine strategies to promote recovery following traumatic brain injuries are currently focused on the use of biomaterials as delivery systems for cells or bioactive molecules. This study shows that cell-free biomimetic scaffolds consisting of radially aligned electrospun poly-l/dl lactic acid (PLA70/30) nanofibers release L-lactate and reproduce the 3D organization and supportive function of radial glia embryonic neural stem cells. The topology of PLA nanofibers supports neuronal migration while L-lactate released during PLA degradation acts as an alternative fuel for neurons and is required for progenitor maintenance. Radial scaffolds implanted into cavities made in the postnatal mouse brain fostered complete implant vascularization, sustained neurogenesis, and allowed the long-term survival and integration of the newly generated neurons. Our results suggest that the endogenous central nervous system is capable of regeneration through the in vivo dedifferentiation induced by biophysical and metabolic cues, with no need for exogenous cells, growth factors, or genetic manipulation.
authors.affiliation: Institute for Bioengineering of Catalonia-IBEC, Barcelona, Spain; Dpt. of Pathology and Experimental Therapeutics, Medical School (Bellvitge Campus), University of Barcelona-UB, Barcelona, Spain; CIBER en Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Barcelona, Spain.
authors.forename: Zaida
authors.initials: Z
authors.lastname: Álvarez
authors.affiliation: Institute for Bioengineering of Catalonia-IBEC, Barcelona, Spain; Dpt. Material Science and Metallurgical Engineering, Technical University of Catalonia-UPC, Barcelona, Spain; CIBER en Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Barcelona, Spain.
authors.forename: Oscar
authors.initials: O
authors.lastname: Castaño
authors.affiliation: Dpt. of Pathology and Experimental Therapeutics, Medical School (Bellvitge Campus), University of Barcelona-UB, Barcelona, Spain.
authors.forename: Alba A
authors.initials: AA
authors.lastname: Castells
authors.affiliation: Institute for Bioengineering of Catalonia-IBEC, Barcelona, Spain; CIBER en Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Barcelona, Spain.
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Mateos-Timoneda
authors.affiliation: Institute for Bioengineering of Catalonia-IBEC, Barcelona, Spain.
authors.forename: Josep A
authors.initials: JA
authors.lastname: Planell
authors.affiliation: Institute for Bioengineering of Catalonia-IBEC, Barcelona, Spain; Dpt. Material Science and Metallurgical Engineering, Technical University of Catalonia-UPC, Barcelona, Spain; CIBER en Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Barcelona, Spain.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Engel
authors.affiliation: Dpt. of Pathology and Experimental Therapeutics, Medical School (Bellvitge Campus), University of Barcelona-UB, Barcelona, Spain. Electronic address: salcantara@ub.edu.
authors.forename: Soledad
authors.initials: S
authors.lastname: Alcántara
mesh_terms: D000818:Animals; D040761:Biomimetic Materials / Q000737:chemistry; D001921:Brain / Q000098:blood supply* / Q000473:pathology / Q000502:physiology*; D002478:Cells, Cultured; D016503:Drug Delivery Systems; D019344:Lactic Acid / Q000008:administration & dosage* / Q000737:chemistry; D051379:Mice; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D018919:Neovascularization, Physiologic; D058953:Neural Stem Cells / Q000166:cytology / Q000637:transplantation*; D055495:Neurogenesis; D011091:Polyesters; D011108:Polymers / Q000737:chemistry; D012038:Regeneration; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide)
keywords: Lactate; Nanofibers; Neural stem cells; Neurogenesis; Regeneration; Vascularization