title: Differentiation of endothelial progenitor cells into endothelial cells by heparin-modified supramolecular pluronic nanogels encapsulating bFGF and complexed with VEGF165 genes.
abstract: Specific genes and growth factors are involved in stem cell differentiation. In this study, we fabricated a delivery carrier for both protein and gene delivery that was introduced into human endothelial progenitor cells (EPCs). The highly negative charge carried by the heparin-modified pluronic nanogels allowed for binding to growth factors and localization in the core of nanogels. The residues of negatively charged heparin can complex with positively charged cationic materials, making it suitable for gene delivery. Supramolecular nanogels can be easily encapsulated the hydrophilic drugs and highly positive surfaces can be complexed with negative charge carrying plasmid DNA (pDNA). The size distribution, gel retardation, and denaturation of encapsulated growth factors and supramolecular nanogels modified with heparin were evaluated. The supramolecular nanogels containing basic fibroblast growth factors and complexing VEGF165 pDNA internalized into EPCs have been well formed vascular formation in matrigel gels. Proteins and genes introduced into EPCs using nanogels promoted neovascularization in an animal model of limb ischemia. EPCs that differentiated into endothelial cells both in vitro and in vivo were tested.
authors.affiliation: Department of Biomedical Science, College of Life Science, CHA University, 606-16 Yeoksam 1-dong, Kangnam-gu, Seoul 135-081, Republic of Korea.
authors.forename: Han Na
authors.initials: HN
authors.lastname: Yang
authors.affiliation: Department of Molecular Science and Technology, Ajou University, San 5, Woncheon, Yeongtong, Suwon 443-749, Republic of Korea.
authors.forename: Jong Hoon
authors.initials: JH
authors.lastname: Choi
authors.affiliation: Department of Biomedical Science, College of Life Science, CHA University, 606-16 Yeoksam 1-dong, Kangnam-gu, Seoul 135-081, Republic of Korea.
authors.forename: Ji Sun
authors.initials: JS
authors.lastname: Park
authors.affiliation: Department of Biomedical Science, College of Life Science, CHA University, 606-16 Yeoksam 1-dong, Kangnam-gu, Seoul 135-081, Republic of Korea.
authors.forename: Su Yeon
authors.initials: SY
authors.lastname: Jeon
authors.affiliation: Department of Molecular Science and Technology, Ajou University, San 5, Woncheon, Yeongtong, Suwon 443-749, Republic of Korea. Electronic address: kdp@ajou.ac.kr.
authors.forename: Ki Dong
authors.initials: KD
authors.lastname: Park
authors.affiliation: Department of Biomedical Science, College of Life Science, CHA University, 606-16 Yeoksam 1-dong, Kangnam-gu, Seoul 135-081, Republic of Korea. Electronic address: pkh0410@cha.ac.kr.
authors.forename: Keun-Hong
authors.initials: KH
authors.lastname: Park
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D004247:DNA / Q000008:administration & dosage* / Q000235:genetics; D004337:Drug Carriers / Q000737:chemistry; D042783:Endothelial Cells / Q000166:cytology* / Q000378:metabolism; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000008:administration & dosage*; D006493:Heparin / Q000737:chemistry*; D006801:Humans; D008807:Mice, Inbred BALB C; D000080385:Nanogels; D018919:Neovascularization, Physiologic; D020442:Poloxamer / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry; D011094:Polyethyleneimine / Q000737:chemistry; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D014162:Transfection; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics*
chemical_list: D004337:Drug Carriers; D000080385:Nanogels; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; C413692:polyethylene glycol polyethyleneimine nanogel; D016222:Fibroblast Growth Factor 2; D020442:Poloxamer; D011092:Polyethylene Glycols; D011094:Polyethyleneimine; D006493:Heparin; D004247:DNA
keywords: Differentiation; EPCs; Genes; Growth factor; Nanogel