title: Synthesis of PNA oligoether conjugates.
abstract: Several different approaches have been explored for conjugation of oligoethers to PNA with internally or N-terminal placed diaminopropionic acid residues. Single and double conjugation of 2-(2-(2-aminoethoxy)ethoxy)ethanol was obtained using carbonyldimidazole. Using a post PNA-assembly coupling procedure the building block 2-(2-(2-(benzoyloxy)ethoxy)ethoxy)acetic acid multiple attachment of 2-(2-(2-hydroxyethoxy)ethoxy)acetyl groups to both N-terminal and β-amino groups of inserted diaminopropionic acids residues was achieved. Use of a new oligoether functionalized amino acid allows inclusion of oligoether conjugates during on-line machine assisted synthesis which also allowed combination of methods for attachment of different oligoethers and co-conjugation of neocuproine as well as conjugation of an aminosugar.
authors.affiliation: Department of Biosciences and Nutrition, Karolinska Institutet, Novum, Hälsovägen Huddinge 7, 14183, Sweden. alice.ghidini@ki.se.
authors.forename: Alice
authors.initials: A
authors.lastname: Ghidini
authors.affiliation: ICL-IP Terneuzen, Frankrijkweg BJ, Terneuzen 6 4538, The Netherlands. SteunenbergP@icl-ip.com.
authors.forename: Peter
authors.initials: P
authors.lastname: Steunenberg
authors.affiliation: Department of Biosciences and Nutrition, Karolinska Institutet, Novum, Hälsovägen Huddinge 7, 14183, Sweden. merita.murtola@ki.se.
authors.forename: Merita
authors.initials: M
authors.lastname: Murtola
authors.affiliation: Department of Biosciences and Nutrition, Karolinska Institutet, Novum, Hälsovägen Huddinge 7, 14183, Sweden. roger.stromberg@ki.se.
authors.forename: Roger
authors.initials: R
authors.lastname: Strömberg
mesh_terms: D060326:Chemistry Techniques, Synthetic; D004986:Ether / Q000737:chemistry*; D020135:Peptide Nucleic Acids / Q000138:chemical synthesis*
chemical_list: D020135:Peptide Nucleic Acids; D004986:Ether