title: [Quantitative detection of serum procollagen III N-terminal peptide chemiluminescence enzyme immunoassay].
abstract: OBJECTIVE
  To establish chemiluminescence enzyme immunoassay (CLEIA) for quantitative detection of procollagen III N-terminal peptide (P III NP) in serum.
  
  
  METHODS
  A sandwich reaction was preformed with horseradish peroxidase labeled monoclonal antibody of P III NP as the catalytic enzyme and the luminol as the luminescence reagent. Several reactions liquid's concentration and reaction conditions were optimized. The method was evaluated in all aspects such as linear range, sensitivity, specificity, stability and so on. The CLEIA was compared with imported ELISA kits, by detecting clinical serum.
  
  
  RESULTS
  The linear range was 0.8-85 ng/ml. The detection limit was 0.5 ng/ml. Inter-assay and intra-assay RSD were both less than 10%. The recoveries of three different spiked concentration samples were 96.2%, 91.2% and 101.1%. After stored at 4 degrees C and 37 degrees C for 3, 5, 7 days, the analysis showed correlation coefficient higher than 0.99 and RSD lower than 6%. The detected results of clinical sera with CLEIA closely corresponded to those with imported ELISA.
  
  
  CONCLUSIONS
  Established CLEIA for quantity determination of serum P III NP has high accuracy, sensitivity and repeatability.
authors.forename: Jia
authors.initials: J
authors.lastname: Liu
authors.forename: Yan-Qing
authors.initials: YQ
authors.lastname: Feng
authors.forename: Yong-Ji
authors.initials: YJ
authors.lastname: Song
authors.forename: Jun
authors.initials: J
authors.lastname: Hou
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.forename: Ai-Xia
authors.initials: AX
authors.lastname: Liu
authors.forename: Jing-Xia
authors.initials: JX
authors.lastname: Guo
authors.forename: Jun
authors.initials: J
authors.lastname: Xu
authors.forename: Hong-Shan
authors.initials: HS
authors.lastname: Wei
authors.forename: Bo-An
authors.initials: BA
authors.lastname: Li
mesh_terms: D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation / Q000379:methods*; D005260:Female; D006801:Humans; D008103:Liver Cirrhosis / Q000097:blood* / Q000175:diagnosis; D049449:Luminescence; D008163:Luminescent Measurements / Q000379:methods*; D008297:Male; D008875:Middle Aged; D010446:Peptide Fragments / Q000097:blood* / Q000737:chemistry; D011347:Procollagen / Q000097:blood* / Q000737:chemistry; D012680:Sensitivity and Specificity
chemical_list: D000911:Antibodies, Monoclonal; D010446:Peptide Fragments; D011347:Procollagen; C041876:procollagen Type III-N-terminal peptide