title: Virus-induced gene silencing and Agrobacterium tumefaciens-mediated transient expression in Nicotiana tabacum.
abstract: Virus-induced gene silencing (VIGS) is a rapid method for transient silencing of plant genes. In this chapter, we describe the methodology for Tobacco rattle virus (TRV)-based VIGS in Nicotiana tabacum. In combination with subsequent co-expression of the tomato immune receptor Ve1 and the corresponding Verticillium effector Ave1 through Agrobacterium tumefaciens-mediated transient transformation (agroinfiltration), we established a rapid system for assessing the requirement of candidate plant genes for Ve1-mediated immune signaling.
authors.affiliation: Laboratory of Phytopathology, Wageningen University, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Zhang
authors.forename: Bart P H J
authors.initials: BP
authors.lastname: Thomma
mesh_terms: D016960:Agrobacterium tumefaciens / Q000378:metabolism*; D015870:Gene Expression; D020868:Gene Silencing; D010088:Oxidoreductases / Q000378:metabolism; D010942:Plant Viruses / Q000378:metabolism*; D014026:Nicotiana / Q000201:enzymology / Q000254:growth & development / Q000382:microbiology* / Q000821:virology*
chemical_list: D010088:Oxidoreductases; C022398:phytoene dehydrogenase