title: Quercetin-3-O-β-D-glucuronide isolated from Polygonum aviculare inhibits cellular senescence in human primary cells.
abstract: Cellular senescence is known to contribute to tissue aging, a variety of age-related diseases, tissue regeneration, and cancer. Therefore, aging intervention might be useful for prevention of aging as well as age-related disease. In this study, we investigated compounds from Polygonum aviculare to determine if they inhibited cellular senescence in human primary cells, human dermal fibroblasts (HDFs) and human umbilical vein endothelial cells (HUVECs). Ten compounds from P. aviculare were purified and their inhibitory effects on adriamycin-induced cellular senescence were measured by observing senescence-associated β-galactosidase (SA-β-gal) activity and reactive oxygen species. Among them, compound 9 (quercetin-3-O-β-D-glucuronide) showed inhibitory effects against cellular senescence in HDFs and HUVECs treated with adriamycin. Additionally, compound 9 rescued replicative senescence in HDFs and HUVECs. These data imply that compound 9 represses cellular senescence in human primary cells and might be useful for the development of dietary supplements or cosmetics that ameliorate tissue aging or aging-associated diseases.
authors.affiliation: Department of Biochemistry and Molecular Biology, College of Medicine, Yeungnam University, 170 Hyunchung-Ro, Daegu, 705-717, Republic of Korea.
authors.forename: Hyo Hyun
authors.initials: HH
authors.lastname: Yang
authors.forename: Kyoung
authors.initials: K
authors.lastname: Hwangbo
authors.forename: Ming Shan
authors.initials: MS
authors.lastname: Zheng
authors.forename: Jung Hee
authors.initials: JH
authors.lastname: Cho
authors.forename: Jong-Keun
authors.initials: JK
authors.lastname: Son
authors.forename: Hwa Young
authors.initials: HY
authors.lastname: Kim
authors.forename: Suk Hwan
authors.initials: SH
authors.lastname: Baek
authors.forename: Hyung Chul
authors.initials: HC
authors.lastname: Choi
authors.forename: So Young
authors.initials: SY
authors.lastname: Park
authors.forename: Jae-Ryong
authors.initials: JR
authors.lastname: Kim
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000187:drug effects*; D055808:Drug Discovery; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D020834:Ethnopharmacology; D020719:Glucuronides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000187:drug effects; D006801:Humans; D055670:Medicine, Korean Traditional; D015394:Molecular Structure; D009994:Osmolar Concentration; D035261:Plant Components, Aerial / Q000737:chemistry; D028502:Polygonum / Q000737:chemistry; D011794:Quercetin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors / Q000378:metabolism; D012867:Skin / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D015595:Skin Aging / Q000187:drug effects*; D001616:beta-Galactosidase / Q000037:antagonists & inhibitors / Q000378:metabolism
chemical_list: D000975:Antioxidants; D020719:Glucuronides; D017382:Reactive Oxygen Species; C443401:quercetin 3-O-glucuronide; D011794:Quercetin; C061901:GLB1 protein, human; D001616:beta-Galactosidase