title: Antiadhesive properties of arabinogalactan protein from ribes nigrum seeds against bacterial adhesion of Helicobacter pylori.
abstract: Fruit extracts from black currants (Ribes nigrum L.) are traditionally used for treatment of gastritis based on seed polysaccharides that inhibit the adhesion of Helicobacter pylori to stomach cells. For detailed investigations an arabinogalactan protein (F2) was isolated from seeds and characterized concerning molecular weight, carbohydrate, amino acid composition, linkage, configuration and reaction with β-glucosyl Yariv. Functional testing of F2 was performed by semiquantitative in situ adhesion assay on sections of human gastric mucosa and by quantitative in vitro adhesion assay with FITC-labled H. pylori strain J99 and human stomach AGS cells. Bacterial adhesins affected were identified by overlay assay with immobilized ligands. ¹²⁵I-radiolabeled F2 served for binding studies to H. pylori and interaction experiments with BabA and SabA. F2 had no cytotoxic effects against H. pylori and AGS cells; but inhibited bacterial binding to human gastric cells. F2 inhibited the binding of BabA and fibronectin-binding adhesin to its specific ligands. Radiolabeled F2 bound non-specifically to different strains of H. pylori; and to BabA deficient mutant. F2 did not lead to subsequent feedback regulation or increased expression of adhesins or virulence factors. From these data the non-specific interactions between F2 and the H. pylori lead to moderate antiadhesive effects.
authors.affiliation: Institute of Pharmaceutical Biology and Phytochemistry, University of Münster, D-48149 Münster, Germany. jutta.messing@uni-muenster.de.
authors.forename: Jutta
authors.initials: J
authors.lastname: Messing
authors.affiliation: Institute of Pharmaceutical Biology and Phytochemistry, University of Münster, D-48149 Münster, Germany. m_niehues@hotmail.com.
authors.forename: Michael
authors.initials: M
authors.lastname: Niehues
authors.affiliation: Department of Medical Biochemistry and Biophysics, Umeå University, Umeå, SE-901 87, Sweden. anna.shevtsova@medchem.umu.se.
authors.forename: Anna
authors.initials: A
authors.lastname: Shevtsova
authors.affiliation: Department of Medical Biochemistry and Biophysics, Umeå University, Umeå, SE-901 87, Sweden. thomas.boren@medchem.umu.se.
authors.forename: Thomas
authors.initials: T
authors.lastname: Borén
authors.affiliation: Institute of Pharmaceutical Biology and Phytochemistry, University of Münster, D-48149 Münster, Germany. ahensel@uni-muenster.de.
authors.forename: Andreas
authors.initials: A
authors.lastname: Hensel
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics; D001422:Bacterial Adhesion / Q000187:drug effects*; D002241:Carbohydrates / Q000737:chemistry; D002460:Cell Line; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D016480:Helicobacter pylori / Q000187:drug effects* / Q000235:genetics; D006801:Humans; D015394:Molecular Structure; D008970:Molecular Weight; D009088:Mucoproteins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D031965:Ribes / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry*
chemical_list: D018829:Adhesins, Bacterial; D002241:Carbohydrates; D009088:Mucoproteins; D010940:Plant Proteins; C006619:arabinogalactan proteins