title: Immunoreagents and competitive assays to fludioxonil.
abstract: Fludioxonil is a new-generation fungicide widely used for postharvest fruit protection. The aim of this study was to produce hitherto unreported immunoreagents for Fludioxonil analysis by immunoassay. Derivatives of this agrochemical were synthesized with different linker tethering sites. Those functionalized haptens were activated, and the purified active esters were efficiently conjugated to different carrier proteins for immunogen and assay antigen preparation. Antibodies to Fludioxonil were raised in rabbits, and their selectivity and affinity were characterized, revealing the significance of the linker. Those antibodies were evaluated using homologous and heterologous conjugates by direct and indirect competitive ELISA formats. Finally, a pair of immunoreagents was identified showing an IC50 value for Fludioxonil of 5.7 μg/L.
authors.affiliation: Department of Biotechnology, Institute of Agrochemistry and Food Technology, Consejo Superior de Investigaciones Científicas (IATA-CSIC) , Agustı́ Escardino 7, 46980 Paterna, València, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Abad-Fuentes
authors.forename: Consuelo
authors.initials: C
authors.lastname: Agulló
authors.forename: Francesc A
authors.initials: FA
authors.lastname: Esteve-Turrillas
authors.forename: Antonio
authors.initials: A
authors.lastname: Abad-Somovilla
authors.forename: Josep V
authors.initials: JV
authors.lastname: Mercader
mesh_terms: D000818:Animals; D000906:Antibodies / Q000032:analysis; D004149:Dioxoles / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation / Q000379:methods*; D005659:Fungicides, Industrial / Q000032:analysis*; D011758:Pyrroles / Q000032:analysis*; D011817:Rabbits
chemical_list: D000906:Antibodies; D004149:Dioxoles; D005659:Fungicides, Industrial; D011758:Pyrroles; C108339:fludioxonil