title: The do's and don'ts of effectoromics.
abstract: Effectoromics, a high-throughput functional genomics approach that uses effectors to probe plant germplasm to detect R genes, has proven a potent contribution to modern resistance breeding. Advantages of effectoromics are summarized in four aspects: (1) accelerating R gene identification; (2) distinguishing functional redundancy; (3) detecting recognition specificity and (4) assisting in R gene deployment. In this manuscript, we provide suggestions as well as some reminders for applying effectoromics in the breeding process. The two routine functional assays that are widely used, agroinfiltration and agroinfection, are presented. We briefly explain their advantages and disadvantages and provide protocols for applying them in the model system Nicotiana benthamiana as well as in potato (Solanum tuberosum).
authors.affiliation: Wageningen UR Plant Breeding, Wageningen University & Research Centre, 386, 6700 AJ, Wageningen, The Netherlands.
authors.forename: Juan
authors.initials: J
authors.lastname: Du
authors.forename: Vivianne G A A
authors.initials: VG
authors.lastname: Vleeshouwers
mesh_terms: D060054:Agrobacterium / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism; D003001:Cloning, Molecular; D060467:Disease Resistance / Q000276:immunology; D017343:Genes, Plant; D023281:Genomics / Q000379:methods*; D010935:Plant Diseases / Q000235:genetics / Q000276:immunology; D010940:Plant Proteins / Q000378:metabolism; D011198:Solanum tuberosum / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D014026:Nicotiana / Q000235:genetics* / Q000276:immunology / Q000382:microbiology
chemical_list: D001426:Bacterial Proteins; D010940:Plant Proteins