title: Apoptosis mediated anti-proliferative effect of compound isolated from Cassia auriculata leaves against human colon cancer cell line.
abstract: A compound was isolated from Cassia auriculata leaves and characterized by high-performance liquid chromatography (HPLC), liquid chromatography mass spectrometry (LC-MS), UV-vis spectroscopy (UV-vis), Fourier transform infrared spectroscopy (FT-IR) and nuclear magnetic resonance spectroscopy (NMR). The in vitro anticancer effect of the compound isolated from C. auriculata was evaluated in human colon cancer cells HCT 15 by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, cytotoxicity, nuclear morphology analysis and measurement of lactate dehydrogenase. The isolated compound 4-(2,5 dichlorobenzyl)-2,3,4,5,6,7 hexahydro7(4 methoxyphenyl)benzo[h][1,4,7] triazecin8(1H)-one showed 50% inhibition of HCT 15 cells when tested at 20μg/ml after 24h incubation. Cytotoxicity, nuclear morphology and lactate dehydrogenase assays clearly show potent anticancer activity of the isolated compound against colon cancer. Thus, the in vitro findings suggest that the compound isolated from C. auriculata leaves have potent anti-cancer properties with possible clinical applications.
authors.affiliation: Department of Animal Health and Management, Alagappa University, Karaikudi 630 003, Tamil Nadu, India.
authors.forename: M
authors.initials: M
authors.lastname: Esakkirajan
authors.affiliation: Department of Animal Health and Management, Alagappa University, Karaikudi 630 003, Tamil Nadu, India. Electronic address: prabhunm71@gmail.com.
authors.forename: N M
authors.initials: NM
authors.lastname: Prabhu
authors.affiliation: Department of Zoology, University of Madras, Guindy Campus, Chennai 600 025, Tamil Nadu, India. Electronic address: manikandanramar@yahoo.co.in.
authors.forename: R
authors.initials: R
authors.lastname: Manikandan
authors.affiliation: Department of Zoology, University of Madras, Guindy Campus, Chennai 600 025, Tamil Nadu, India.
authors.forename: M
authors.initials: M
authors.lastname: Beulaja
authors.affiliation: Department of Zoology, University of Madras, Guindy Campus, Chennai 600 025, Tamil Nadu, India.
authors.forename: D
authors.initials: D
authors.lastname: Prabhu
authors.affiliation: Centre for Ocean Research, Sathyabama University, Chennai 600 119, Tamil Nadu, India.
authors.forename: K
authors.initials: K
authors.lastname: Govindaraju
authors.affiliation: Department of Biotechnology, School of Chemical and Biotechnology, SASTRA University, Thanjavur 613 401, Tamil Nadu, India.
authors.forename: R
authors.initials: R
authors.lastname: Thiagarajan
authors.affiliation: Department of Zoology, University of Madras, Guindy Campus, Chennai 600 025, Tamil Nadu, India.
authors.forename: C
authors.initials: C
authors.lastname: Arulvasu
authors.affiliation: Department of Zoology, University of Madras, Guindy Campus, Chennai 600 025, Tamil Nadu, India.
authors.forename: G
authors.initials: G
authors.lastname: Dhanasekaran
authors.affiliation: Department of Zoology, University of Madras, Guindy Campus, Chennai 600 025, Tamil Nadu, India.
authors.forename: D
authors.initials: D
authors.lastname: Dinesh
authors.affiliation: Department of Zoology, University of Madras, Guindy Campus, Chennai 600 025, Tamil Nadu, India.
authors.forename: G
authors.initials: G
authors.lastname: Babu
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D002366:Cassia / Q000737:chemistry*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D003110:Colonic Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D018515:Plant Leaves / Q000737:chemistry*
chemical_list: D000972:Antineoplastic Agents, Phytogenic
keywords: Anticancer; Apoptosis; Cancer; Cassia auriculata; HCT 15; Natural product