title: A crucial role of L-selectin in C protein-induced experimental polymyositis in mice.
abstract: OBJECTIVE
  To investigate the role of adhesion molecules in C protein-induced myositis (CIM), a murine model of polymyositis (PM).
  
  
  METHODS
  CIM was induced in wild-type mice, L-selectin-deficient (L-selectin(-/-) ) mice, intercellular adhesion molecule 1 (ICAM-1)-deficient (ICAM-1(-/-) ) mice, and mice deficient in both L-selectin and ICAM-1 (L-selectin(-/-) ICAM-1(-/-) mice). Myositis severity, inflammatory cell infiltration, and messenger RNA expression in the inflamed muscles were analyzed. The effect of dendritic polyglycerol sulfate, a synthetic inhibitor that suppresses the function of L-selectin and endothelial P-selectin, was also examined.
  
  
  RESULTS
  L-selectin(-/-) mice and L-selectin(-/-) ICAM-1(-/-) mice developed significantly less severe myositis compared to wild-type mice, while ICAM-1 deficiency did not inhibit the development of myositis. L-selectin(-/-) mice that received wild-type T cells developed myositis. Treatment with dendritic polyglycerol sulfate significantly diminished the severity of myositis in wild-type mice compared to treatment with control.
  
  
  CONCLUSIONS
  These data indicate that L-selectin plays a major role in the development of CIM, whereas ICAM-1 plays a lesser role, if any, in the development of CIM. L-selectin-targeted therapy may be a candidate for the treatment of PM.
authors.affiliation: Kanazawa University, Kanazawa, Japan.
authors.forename: Kyosuke
authors.initials: K
authors.lastname: Oishi
authors.forename: Yasuhito
authors.initials: Y
authors.lastname: Hamaguchi
authors.forename: Takashi
authors.initials: T
authors.lastname: Matsushita
authors.forename: Minoru
authors.initials: M
authors.lastname: Hasegawa
authors.forename: Naoko
authors.initials: N
authors.lastname: Okiyama
authors.forename: Jens
authors.initials: J
authors.lastname: Dernedde
authors.forename: Marie
authors.initials: M
authors.lastname: Weinhart
authors.forename: Rainer
authors.initials: R
authors.lastname: Haag
authors.forename: Thomas F
authors.initials: TF
authors.lastname: Tedder
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Takehara
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Kohsaka
authors.forename: Manabu
authors.initials: M
authors.lastname: Fujimoto
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology; D019264:Adoptive Transfer; D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002352:Carrier Proteins / Q000494:pharmacology*; D002465:Cell Movement / Q000276:immunology; D004195:Disease Models, Animal; D005260:Female; D005620:Freund's Adjuvant / Q000494:pharmacology; D005990:Glycerol / Q000494:pharmacology; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000276:immunology*; D019041:L-Selectin / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D011108:Polymers / Q000494:pharmacology; D017285:Polymyositis / Q000139:chemically induced / Q000235:genetics / Q000276:immunology*; D012720:Severity of Illness Index; D000099163:Myosin Binding Protein C
chemical_list: D000276:Adjuvants, Immunologic; D002352:Carrier Proteins; D005620:Freund's Adjuvant; D005990:Glycerol; D018799:Intercellular Adhesion Molecule-1; D019041:L-Selectin; D011108:Polymers; D000099163:Myosin Binding Protein C; C090509:Icam1 protein, mouse; C043941:polyglycerol