title: MicroRNA expression profiles of peripheral blood mononuclear cells in patients with systemic lupus erythematosus.
abstract: Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by the presence of autoantibodies against numerous self-antigens. Evidence underlines the importance of microRNAs (miRNAs) in the pathogenesis of SLE, but the exact etiology of this disease is unknown. Therefore, this study was conducted to explore regulation of abnormal miRNAs in SLE using microarray analysis. Peripheral blood mononuclear cells (PBMCs) from SLE patients and their matched controls were isolated appropriately. Total RNAs from each cell sample were extracted and used for microarray analysis. A total of 29 miRNAs were identified to be down-regulated in PBMCs of SLE patients as compared with healthy controls (P<0.05, fold change>2). No significant up-regulated miRNAs were found in SLE patients. Results of gene ontology analysis indicated that the potential target genes of these miRNAs mainly enriched in the development process, transcription regulator activity, ligand binding, etc. Meanwhile, these putative genes were also found to be involved in diverse signaling transduction pathways, including multiple cancer pathways, Wnt and mitogen-activated protein kinase signaling pathways, etc. This study revealed possible dysregulated biological processes and pathways in SLE that were targeted by the differentially expressed miRNAs, indicating the involvement of these miRNAs in the pathogenesis of SLE.
authors.affiliation: Department of Rheumatology and Immunology, Shengjing Hospital, China Medical University, Shenyang 110004, People's Republic of China.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Liu
authors.affiliation: Department of Rheumatology and Immunology, Shengjing Hospital, China Medical University, Shenyang 110004, People's Republic of China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Department of Rheumatology and Immunology, Shengjing Hospital, China Medical University, Shenyang 110004, People's Republic of China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Department of Rheumatology and Immunology, Shengjing Hospital, China Medical University, Shenyang 110004, People's Republic of China. Electronic address: xiaofei.wang@hotmail.com.
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Wang
mesh_terms: D000328:Adult; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008180:Lupus Erythematosus, Systemic / Q000503:physiopathology*; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D046228:Microarray Analysis; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction; D059467:Transcriptome
chemical_list: D035683:MicroRNAs
keywords: Gene ontology analysis; Microarray; Pathway analysis; SLE; miRNA