title: Bone marrow plasma macrophage inflammatory protein protein-1 alpha(MIP-1 alpha) and sclerostin in multiple myeloma: relationship with bone disease and clinical characteristics.
abstract: The aim of the study was to investigate the expression of MIP-1 alpha and sclerostin in bone marrow of patients with multiple myeloma (MM), the possible association of the sclerostin and MIP-1 alpha with MBD and the clinical characteristics. 53 patients (29 M, 24 F), median age 64 years was studied. MIP-1 alpha, sclerostin and bone-specific alkaline phosphatase (bALP) levels were quantified using an enzyme-linked immunosorbent assay (ELISA). Sclerostin and MIP-1 alpha mRNA expression was determined by RT-PCR. PTH and 1,25(OH) 2D3 levels were measured with an electrochemiluminescence immunoassay. The sclerostin and MIP-1 alpha concentrations in patients with MM were higher than those in the controls. RT-PCR analysis verified that the bone marrow mononuclear cells (BMMNCs) of most patients showed sclerostin and MIP-1 alpha mRNA expression. The sclerostin and MIP-1 alpha levels in patients with ISS stage III disease were significantly higher than those in patients with ISS stage II disease (p=0.01 and 0.06). The sclerostin and MIP-1 alpha levels in patients with BMD in group C were significantly higher than those in group A+B. There was positive correlation between sclerostin levels and MIP-1 alpha, beta2-microglobulin and aCa levels. A negative association was seen between sclerostin levels and bALP, HB and ALB levels. The MM patients with high sclerostin levels (>0.72 ng/ml) had significantly shorter median survival than those with low sclerostin levels (≤0.72 ng/ml) (χ(2)=7.574, p=0.006). Our findings support the positive relationship between sclerostin levels and MIP-1alpha levels deserve further detailed research.
authors.affiliation: Guangxi Medical University, Nanning, China; Department of Hematology, Affiliated Hospital of Guilin Medical College, Guilin, China.
authors.forename: Xiao-Tao
authors.initials: XT
authors.lastname: Wang
authors.affiliation: Department of Hematology, Affiliated Hospital of Guilin Medical College, Guilin, China.
authors.forename: Yu-Chan
authors.initials: YC
authors.lastname: He
authors.affiliation: Department of Hematology, Affiliated Hospital of Guilin Medical College, Guilin, China.
authors.forename: Si-Yao
authors.initials: SY
authors.lastname: Zhou
authors.affiliation: Department of Hematology, Affiliated Hospital of Guilin Medical College, Guilin, China.
authors.forename: Jing-zi
authors.initials: JZ
authors.lastname: Jiang
authors.affiliation: Guangxi Medical University, Nanning, China.
authors.forename: Yu-Mei
authors.initials: YM
authors.lastname: Huang
authors.affiliation: Guangxi Medical University, Nanning, China.
authors.forename: Yu-Zhen
authors.initials: YZ
authors.lastname: Liang
authors.affiliation: Guangxi Medical University, Nanning, China. Electronic address: wxttjl@126.com.
authors.forename: Yong-Rong
authors.initials: YR
authors.lastname: Lai
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000293:Adolescent; D000328:Adult; D000368:Aged; D015519:Bone Density; D001847:Bone Diseases / Q000209:etiology / Q000378:metabolism*; D001853:Bone Marrow / Q000737:chemistry*; D019485:Bone Morphogenetic Proteins / Q000032:analysis*; D054405:Chemokine CCL3 / Q000032:analysis*; D005260:Female; D005819:Genetic Markers; D006801:Humans; D008297:Male; D008875:Middle Aged; D009101:Multiple Myeloma / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D009367:Neoplasm Staging; D012709:Serum Albumin / Q000032:analysis; D051176:beta Catenin / Q000502:physiology
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D019485:Bone Morphogenetic Proteins; D054405:Chemokine CCL3; D005819:Genetic Markers; C422903:SOST protein, human; D012709:Serum Albumin; D051176:beta Catenin
keywords: MM-related bone disease; Macrophage inflammatory protein-1 alpha; Multiple Myeloma; Sclerostin