title: Biocomposite scaffolds based on electrospun poly(3-hydroxybutyrate) nanofibers and electrosprayed hydroxyapatite nanoparticles for bone tissue engineering applications.
abstract: The electrospinning technique combined with the electrospraying process provides a straightforward and versatile approach for the fabrication of novel nanofibrous biocomposite scaffolds with structural, mechanical, and biological properties potentially suitable for bone tissue regeneration. In this comparative investigation, three types of poly(3-hydroxybutyrate) (PHB)-based scaffolds were engineered: (i) PHB mats by electrospinning of a PHB solution, (ii) mats of PHB/hydroxyapatite nanoparticle (nHA) blends by electrospinning of a mixed solution containing PHB and nHAs, and (iii) mats constituted of PHB nanofibers and nHAs by simultaneous electrospinning of a PHB solution and electrospraying of a nHA dispersion. Scaffolds based on PHB/nHA blends displayed improved mechanical properties compared to those of neat PHB mats, due to the incorporation of nHAs within the fibers. The electrospinning/electrospraying approach afforded biocomposite scaffolds with lower mechanical properties, due to their higher porosity, but they displayed slightly better biological properties. In the latter case, the bioceramic, i.e. nHAs, largely covered the fiber surface, thus allowing for a direct exposure to cells. The 21 day-monitoring through the use of MTS assays and SEM analyses demonstrated that human mesenchymal stromal cells (hMSCs) remained viable on PHB/nHA biocomposite scaffolds and proliferated continuously until reaching confluence.
authors.affiliation: Institut de Chimie et des Matériaux Paris-Est, UMR 7182 CNRS, Université Paris-Est Créteil, 2, rue Henri Dunant, 94320 Thiais, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Ramier
authors.affiliation: Laboratoire "Croissance, Réparation et Régénération Tissulaires", EAC 7149 CNRS, Université Paris-Est Créteil, 61, avenue du Général de Gaulle, 94010 Créteil, France.
authors.forename: Thibault
authors.initials: T
authors.lastname: Bouderlique
authors.affiliation: Laboratory of Bioactive Polymers, Institute of Polymers, Bulgarian Academy of Sciences, Acad. G. Bonchev St., bl. 103A, BG-1113 Sofia, Bulgaria.
authors.forename: Olya
authors.initials: O
authors.lastname: Stoilova
authors.affiliation: Laboratory of Bioactive Polymers, Institute of Polymers, Bulgarian Academy of Sciences, Acad. G. Bonchev St., bl. 103A, BG-1113 Sofia, Bulgaria.
authors.forename: Nevena
authors.initials: N
authors.lastname: Manolova
authors.affiliation: Laboratory of Bioactive Polymers, Institute of Polymers, Bulgarian Academy of Sciences, Acad. G. Bonchev St., bl. 103A, BG-1113 Sofia, Bulgaria.
authors.forename: Iliya
authors.initials: I
authors.lastname: Rashkov
authors.affiliation: Institut de Chimie et des Matériaux Paris-Est, UMR 7182 CNRS, Université Paris-Est Créteil, 2, rue Henri Dunant, 94320 Thiais, France.
authors.forename: Valérie
authors.initials: V
authors.lastname: Langlois
authors.affiliation: Institut de Chimie et des Matériaux Paris-Est, UMR 7182 CNRS, Université Paris-Est Créteil, 2, rue Henri Dunant, 94320 Thiais, France.
authors.forename: Estelle
authors.initials: E
authors.lastname: Renard
authors.affiliation: Laboratory of Bioactive Polymers, Institute of Polymers, Bulgarian Academy of Sciences, Acad. G. Bonchev St., bl. 103A, BG-1113 Sofia, Bulgaria.
authors.forename: Patricia
authors.initials: P
authors.lastname: Albanese
authors.affiliation: Institut de Chimie et des Matériaux Paris-Est, UMR 7182 CNRS, Université Paris-Est Créteil, 2, rue Henri Dunant, 94320 Thiais, France. Electronic address: grande@icmpe.cnrs.fr.
authors.forename: Daniel
authors.initials: D
authors.lastname: Grande
mesh_terms: D001672:Biocompatible Materials / Q000494:pharmacology*; D001842:Bone and Bones / Q000187:drug effects*; D002152:Calorimetry, Differential Scanning; D017886:Durapatite / Q000494:pharmacology*; D006801:Humans; D006885:Hydroxybutyrates / Q000494:pharmacology*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D053758:Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D011091:Polyesters / Q000494:pharmacology*; D016062:Porosity; D000091402:Prohibitins; D013314:Stress, Mechanical; D013718:Tensile Strength / Q000187:drug effects; D013818:Thermogravimetry; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D000094122:Polyhydroxybutyrates
chemical_list: D001672:Biocompatible Materials; D017886:Durapatite; D006885:Hydroxybutyrates; D011091:Polyesters; D000091402:Prohibitins; C000717328:PHB protein, human; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates
keywords: Biofunctionalized nanofibers; Biopolymers; Electrospinning; Electrospraying; In-vitro investigation