title: Cytokines as potential biomarkers of liver toxicity induced by Dioscorea bulbifera L.
abstract: The present study is designed to search for the serum cytokine biomarker for liver injury induced by Dioscorea bulbifera L., which is a traditionally used herbal medicine in China. Mice were orally given various doses of ethyl acetate extract (EF) isolated from D. bulbifera for 12 days. The activity of serum alanine/aspartate transaminases (ALT/AST) was increased in EF (400 mg/kg)-treated mice. Histological assessment further confirmed EF (400 mg/kg)-induced liver injury. Results of a cytokine-antibody array demonstrated that there were 10 cytokines up-regulated and 1 cytokine down-regulated in EF (400 mg/kg)-treated mice. Enzyme-linked immunosorbent assay (ELISA) further confirmed the increased level of CD30 ligand (CD30L) and decreased level of interlukin-3 (IL-3) in EF-treated mice. In conclusion, our results demonstrate that the altered expression of CD30L and IL-3 may be potential biomarkers for hepatotoxicity induced by D. bulbifera.
authors.affiliation: Center for Drug Safety Evaluation and Research, Shanghai University of Traditional Chinese Medicine.
authors.forename: Yuchen
authors.initials: Y
authors.lastname: Sheng
authors.forename: Yibo
authors.initials: Y
authors.lastname: Ma
authors.forename: Zhongping
authors.initials: Z
authors.lastname: Deng
authors.forename: Zhengtao
authors.initials: Z
authors.lastname: Wang
authors.forename: Lili
authors.initials: L
authors.lastname: Ji
mesh_terms: D000085:Acetates; D000410:Alanine Transaminase / Q000097:blood; D000704:Analysis of Variance; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D015415:Biomarkers / Q000097:blood*; D053285:CD30 Ligand / Q000097:blood; D056486:Chemical and Drug Induced Liver Injury / Q000097:blood / Q000175:diagnosis*; D016207:Cytokines / Q000097:blood*; D027742:Dioscorea / Q000737:chemistry* / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000633:toxicity; D004797:Enzyme-Linked Immunosorbent Assay; D051379:Mice; D010936:Plant Extracts / Q000633:toxicity*; D016185:Receptors, Interleukin-3 / Q000097:blood
chemical_list: D000085:Acetates; D015415:Biomarkers; D053285:CD30 Ligand; D016207:Cytokines; D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; D016185:Receptors, Interleukin-3; C505775:Tnfsf8 protein, mouse; C007650:ethyl acetate; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase