title: MicroRNA-145 inhibits tumour growth and metastasis in colorectal cancer by targeting fascin-1.
abstract: BACKGROUND
  Recent studies have reported miR-145 dysregulated in colorectal cancer (CRC). In this study, miR-145 profiles were compared between CRC and corresponding non-tumour tissues.
  
  
  METHODS
  The expression levels of miR-145 were analysed in CRC cell lines and tumour tissues by real-time PCR. A luciferase reporter assay confirmed direct targets. The functional effects of miR-145 were examined in transfected CRC cells in vitro and in vivo using established assays.
  
  
  RESULTS
  Downregulation of miR-145 was detected in most primary CRC tumours, and was significantly correlated with a more aggressive phenotype of CRC in patients. In CRC cell lines, ectopic overexpression of miR-145 inhibited cell proliferation, motility and invasion in vitro. Stable overexpression of miR-145 suppressed tumour growth and pulmonary metastasis in vivo. Further studies indicated that miR-145 may directly interact with the 3'-untranslated region (3'-UTR) of Fascin-1 messenger RNA (mRNA), downregulating its mRNA and protein expression levels. In clinical specimens, Fascin-1 expression was negatively correlated with miR-145 expression.
  
  
  CONCLUSIONS
  MiR-145 has a critical role in the inhibition of invasive and metastatic capacities of CRC, probably through directly targeting Fascin-1. This miRNA may be involved in the development and progression of CRC.
authors.affiliation: 1] State Key Laboratory of Oncology in South China, Sun Yat-sen University Cancer Center; Collaborative Innovation Center for Cancer Medicine, 651 Dongfeng Road East, Guangzhou 510060, PR China [2] Department of Surgery, the Fourth Affiliated Hospital of Guangzhou Medical University, Guangzhou 511447, PR China.
authors.forename: Y
authors.initials: Y
authors.lastname: Feng
authors.affiliation: Department of Pathology, First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, PR China.
authors.forename: J
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Department of Cardiology, Zhujiang Hospital of Southern Medical University, Guangzhou 510280, PR China.
authors.forename: C
authors.initials: C
authors.lastname: Ou
authors.affiliation: Department of Surgery, Affiliated Xixiang People's Hospital of Guangdong Medical College, Shenzhen 518102, PR China.
authors.forename: Z
authors.initials: Z
authors.lastname: Deng
authors.affiliation: Department of Cardiology, Zhujiang Hospital of Southern Medical University, Guangzhou 510280, PR China.
authors.forename: M
authors.initials: M
authors.lastname: Chen
authors.affiliation: 1] State Key Laboratory of Oncology in South China, Sun Yat-sen University Cancer Center; Collaborative Innovation Center for Cancer Medicine, 651 Dongfeng Road East, Guangzhou 510060, PR China [2] State Key Laboratory of Targeted Drug for Tumors of Guangdong Province, Guangzhou Double Bioproduct Inc, Guangzhou 510060, PR China.
authors.forename: W
authors.initials: W
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Oncology in South China, Sun Yat-sen University Cancer Center; Collaborative Innovation Center for Cancer Medicine, 651 Dongfeng Road East, Guangzhou 510060, PR China.
authors.forename: L
authors.initials: L
authors.lastname: Li
mesh_terms: D000328:Adult; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D015179:Colorectal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008840:Microfilament Proteins / Q000235:genetics*; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis
chemical_list: D002352:Carrier Proteins; C502658:FSCN1 protein, human; C541010:MIRN145 microRNA, human; D035683:MicroRNAs; D008840:Microfilament Proteins