title: Acute exposure to the vinyl chalcogenide 3-methyl-1-phenyl-2-(phenylseleno)oct-2-en-1-one induces oxidative stress in different brain area of rats.
abstract: The mechanisms that lead to the onset of organoselenium intoxication are still poorly understood. Therefore, in the present study, we investigated the effect of acute administration of 3-methyl-1-phenyl-2-(phenylseleno)oct-2-en-1-one on some parameters of oxidative stress and on the activity of creatine kinase (CK) in different brain areas and on the behaviour in the open field test of 90-day-old male rats. Animals (n = 10/group) were treated intraperitoneally with a single dose of the organoselenium (125, 250 or 500 µg kg(-1) ), and after 1 h of the drug administration, they were exposed to the open field test, and behaviour parameters were recorded. Immediately after they were euthanized, cerebral cortex, hippocampus and cerebellum were dissected for measurement of thiobarbituric acid reactive substances (TBARS), carbonyl, sulfhydryl, catalase (CAT), superoxide dismutase (SOD) and CK activity. Our results showed that the dose of 500 µg kg(-1) of the organoselenium increased the locomotion and rearing behaviours in the open field test. Moreover, the organochalcogen enhanced TBARS in the cerebral cortex and cerebellum and increased the oxidation of proteins (carbonyl) only in the cerebral cortex. Sulfhydryl content was reduced in all brain areas, CAT activity enhanced in the hippocampus and reduced in the cerebellum and SOD activity increased in all brain structures. The organoselenium also inhibited CK activity in the cerebral cortex. Therefore, changes in motor behaviour, redox state and energy homeostasis in rats treated acutely with organoselenium support the hypotheses that the brain is a potential target for the organochalcogen action. Ltd.
authors.affiliation: Departamento de Bioquímica, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Rodrigo Binkowski
authors.initials: RB
authors.lastname: de Andrade
authors.forename: Tanise
authors.initials: T
authors.lastname: Gemelli
authors.forename: Robson B
authors.initials: RB
authors.lastname: Guerra
authors.forename: Caroline
authors.initials: C
authors.lastname: Dani
authors.forename: Clovis Milton Duval
authors.initials: CM
authors.lastname: Wannmacher
authors.forename: Rosane
authors.initials: R
authors.lastname: Gomez
authors.forename: Cláudia
authors.initials: C
authors.lastname: Funchal
mesh_terms: D000818:Animals; D001921:Brain / Q000201:enzymology / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D003402:Creatine Kinase / Q000378:metabolism; D008297:Male; D016566:Organoselenium Compounds / Q000737:chemistry / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: C568342:3-methyl-1-phenyl-2-(phenylseleno)oct-2-en-1-one; D016566:Organoselenium Compounds; D002374:Catalase; D013482:Superoxide Dismutase; D003402:Creatine Kinase
keywords: brain; creatine kinase; open field; organochalcogens; oxidative stress; selenium