title: Overexpression of Arabidopsis HsfA1a enhances diverse stress tolerance by promoting stress-induced Hsp expression.
abstract: Arabidopsis (Arabidopsis thaliana) group A1 heat shock factors (Hsfs), including HsfA1a, are important regulators in the heat shock response. Previous studies have revealed that genetically engineered HsfA1 members result in constitutive Hsf activation and heat shock protein gene (Hsp) expression under normal conditions, eventually enhancing basic thermotolerance in transgenic plants. In this study, we generated transgenic Arabidopsis plants overexpressing HsfA1a. One transgenic line showed a 94-fold increase in the level of HsfA1a mRNA (OE line 1). Overexpressing HsfA1a in OE line 1 plants resulted in higher levels of the inducible expression of Hsp18.2 and Hsp70 genes in response to heat stress, low/high pH changes, and hydrogen peroxide. Analysis of in vivo HsfA1a-promoter binding suggested that the higher level of inducible Hsp expression was mediated by stress-induced activation of elevated levels of HsfA1a in the OE plants. The OE plants showed an increase in tolerance to low/ high pH changes and hydrogen peroxide, in addition to heat shock. These results revealed that overexpressing HsfA1a had positive effects on tolerance to diverse stressors by promoting inducible Hsp expression following stress-induced HsfA1a activation. This study suggests a different mechanism for the activation of genetically engineered Hsfs from that suggested in previous reports, thus providing new insight into complex mechanisms used for achieving stress tolerance by genetic engineering.
authors.affiliation: Laboratory for Conservation and Utilization of Bio-Resources & Key Laboratory for Microbial Resources of the Ministry of Education, School of Life Sciences, Yunnan University, Kunming, China.
authors.forename: J
authors.initials: J
authors.lastname: Qian
authors.affiliation: Laboratory for Conservation and Utilization of Bio-Resources & Key Laboratory for Microbial Resources of the Ministry of Education, School of Life Sciences, Yunnan University, Kunming, China.
authors.forename: J
authors.initials: J
authors.lastname: Chen
authors.affiliation: Laboratory for Conservation and Utilization of Bio-Resources & Key Laboratory for Microbial Resources of the Ministry of Education, School of Life Sciences, Yunnan University, Kunming, China.
authors.forename: Y F
authors.initials: YF
authors.lastname: Liu
authors.affiliation: Laboratory for Conservation and Utilization of Bio-Resources & Key Laboratory for Microbial Resources of the Ministry of Education, School of Life Sciences, Yunnan University, Kunming, China.
authors.forename: L L
authors.initials: LL
authors.lastname: Yang
authors.affiliation: Laboratory for Conservation and Utilization of Bio-Resources & Key Laboratory for Microbial Resources of the Ministry of Education, School of Life Sciences, Yunnan University, Kunming, China.
authors.forename: W P
authors.initials: WP
authors.lastname: Li
authors.affiliation: Laboratory for Conservation and Utilization of Bio-Resources & Key Laboratory for Microbial Resources of the Ministry of Education, School of Life Sciences, Yunnan University, Kunming, China Leminzhang8@yahoo.com.
authors.forename: L M
authors.initials: LM
authors.lastname: Zhang
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000502:physiology; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D000076249:Heat Shock Transcription Factors; D006360:Heat-Shock Proteins / Q000235:genetics*; D018869:Heat-Shock Response; D006358:Hot Temperature; D006861:Hydrogen Peroxide / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D013312:Stress, Physiological; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D029681:Arabidopsis Proteins; D004268:DNA-Binding Proteins; D000076249:Heat Shock Transcription Factors; D006360:Heat-Shock Proteins; D010940:Plant Proteins; D014157:Transcription Factors; D006861:Hydrogen Peroxide