title: Structural characterization and bioactivity of released exopolysaccharides from Lactobacillus plantarum 70810.
abstract: Two released exopolysaccharides (r-EPS1 and r-EPS2) were isolated from the culture supernatant of Lactobacillus plantarum 70810 by ethanol precipitation, anion exchange and gel filtration chromatography and characterization of the structures were conducted. The r-EPS1 and r-EPS2 were homogenous polysaccharides with average molecular weights of, respectively, 204.6 and 202.8kDa, and composed of glucose, mannose and galactose with molar ratios of 18.21:78.76:3.03 and 12.92:30.89:56.19, respectively. Methylation and NMR analysis revealed r-EPS1 was consisted of alpha configuration sugar residues, whereas r-EPS2 contained both alpha and beta configuration sugar residues. Antioxidant and antitumor activities of the two exopolysaccharides were evaluated in vitro. The results showed that r-EPS1 and r-EPS2 exhibited potent antioxidant activities regarding hydroxyl and DPPH radicals scavenging and reducing power assays, and possessed antitumor activities against Caco-2, BGC-823 and HT-29 cells, which suggested that they could be potential sources of antioxidant and antitumor drug.
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Kun
authors.initials: K
authors.lastname: Wang
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Rui
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Chen
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Mei
authors.initials: M
authors.lastname: Jiang
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China. Electronic address: dongms@njau.edu.cn.
authors.forename: Mingsheng
authors.initials: M
authors.lastname: Dong
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology; D018938:Caco-2 Cells; D019073:HT29 Cells; D006801:Humans; D006878:Hydroxides / Q000737:chemistry; D048191:Lactiplantibacillus plantarum / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D010084:Oxidation-Reduction; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D006878:Hydroxides; D011134:Polysaccharides; C031356:hydroxide ion
keywords: Antitumor and antioxidant; Lactobacillus plantarum; Released exopolysaccharides (r-EPS)