title: Detection of the dengue virus NS1 antigen using an enzyme immunoassay.
abstract: Current diagnostic methods for dengue virus (DV) rely primarily on detection of anti-DV antibodies and/or DV RNA by reverse transcriptase (RT) PCR. Several limitations exist however: seroconversion is delayed following infection, and DV RT-PCR assays are not yet readily available. The DV nonstructural protein 1 (NS1) antigen is an alternative acute phase DV biomarker, and here, we evaluated the new InBios (InBios International, Inc., Seattle, WA, USA) DENV Detect(TM) NS1 enzyme-linked immunoassay (ELISA) compared to DV RT-PCR and serology for detection of recent DV infection. We report a positive, negative, and overall percent agreement of 96% (24/25), 86.0% (43/50), and 89.3% (67/75) for the InBios NS1 ELISA compared to DV RT-PCR. Performance of the NS1 ELISA compared to serology for anti-DV IgM antibodies showed a positive, negative, and overall percent agreement of 78.0% (85/109), 90.7% (333/367), and 87.8% (418/476), respectively. Collectively, the InBios NS1 ELISA can be used as an alternative to DV RT-PCR for identification of acute DV infection.
authors.affiliation: Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905, USA.
authors.forename: Neil W
authors.initials: NW
authors.lastname: Anderson
authors.affiliation: Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905, USA.
authors.forename: Deborah J
authors.initials: DJ
authors.lastname: Jespersen
authors.affiliation: Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905, USA.
authors.forename: Leonard
authors.initials: L
authors.lastname: Rollins
authors.affiliation: Focus Diagnostics, Cypress, CA 90630, USA.
authors.forename: Brent
authors.initials: B
authors.lastname: Seaton
authors.affiliation: Focus Diagnostics, Cypress, CA 90630, USA.
authors.forename: Harry E
authors.initials: HE
authors.lastname: Prince
authors.affiliation: Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905, USA. Electronic address: theel.elitza@mayo.edu.
authors.forename: Elitza S
authors.initials: ES
authors.lastname: Theel
mesh_terms: D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000097:blood*; D003715:Dengue / Q000175:diagnosis*; D003716:Dengue Virus / Q000276:immunology / Q000302:isolation & purification*; D003955:Diagnostic Tests, Routine / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012698:Serologic Tests / Q000379:methods; D017361:Viral Nonstructural Proteins / Q000097:blood*
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D017361:Viral Nonstructural Proteins
keywords: Dengue virus; ELISA; NS1 antigen; Serum