title: Determination of sulfadiazine in Jiaozhou Bay using molecularly imprinted solid-phase extraction followed by high-performance liquid chromatography with a diode-array detector.
abstract: A highly selective sample cleanup procedure featuring molecularly imprinted solid-phase extraction (MISPE) was developed for the isolation and determination of sulfadiazine (SDZ) in seawater samples from Jiaozhou Bay, China. The molecularly imprinted polymer (MIP) was prepared using SDZ as the template molecule and methacrylic acid as the functional monomer. The MIP was used as a selective sorbent for the solid-phase extraction of SDZ. An off-line MISPE method followed by high-performance liquid chromatography (HPLC) with diode-array detection was established for the analysis of SDZ. Good linearity of the MISPE column for SDZ standard solutions was obtained within 0-200μgL(-1) (R(2)>0.99). The recoveries of spiked seawater samples were satisfactory as high as 88%. Finally, seven samples in Jiaozhou Bay were determined and there was no sulfadiazine found except #2 and #5 sample. The concentrations were respectively 0.33μgL(-1) and 0.28μgL(-1), and the relative standard deviations were 1.35% and 4.13% (n=3).
authors.affiliation: Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao 266100, PR China; Marine College, Shandong University at Weihai, Weihai 264209, PR China.
authors.forename: Ziru
authors.initials: Z
authors.lastname: Lian
authors.affiliation: Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao 266100, PR China.
authors.forename: Xingliang
authors.initials: X
authors.lastname: He
authors.affiliation: Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao 266100, PR China. Electronic address: jtwang@ouc.edu.cn.
authors.forename: Jiangtao
authors.initials: J
authors.lastname: Wang
mesh_terms: D002681:China; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D057230:Limit of Detection; D016014:Linear Models; D054802:Molecular Imprinting / Q000379:methods*; D015203:Reproducibility of Results; D012623:Seawater / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000379:methods*; D013411:Sulfadiazine / Q000032:analysis* / Q000302:isolation & purification; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000302:isolation & purification
chemical_list: D014874:Water Pollutants, Chemical; D013411:Sulfadiazine
keywords: Jiaozhou Bay; Molecularly imprinted polymer; Seawater; Solid-phase extraction; Sulfadiazine