title: Postnatal regulation of 15-hydroxyprostaglandin dehydrogenase in the rat kidney.
abstract: Cyclooxygenase 2 (COX-2) has an established role in postnatal kidney development. 15-Hydroxyprostaglandin dehydrogenase (15-PGDH) is recently identified as an endogenous inhibitor of COX-2, limiting the production of COX-2-derived prostanoids in several pathological conditions. The present study was undertaken to examine the regulation of renal 15-PGDH expression during postnatal kidney development in rats compared with COX-2. qRT-PCR and immunoblotting demonstrated that 15-PGDH mRNA and protein in the kidney were present in neonates, peaked in the second postnatal week, and then declined sharply to very low level in adulthood. Immunostaining demonstrated that at the second postnatal week, renal 15-PGDH protein was predominantly found in the proximal tubules stained positive for Na/H exchanger 3 and brush borders (periodic acid-Schiff), whereas COX-2 protein was restricted to macular densa and adjacent thick ascending limbs. Interestingly, in the fourth postnatal week, 15-PGDH protein was redistributed to thick ascending limbs stained positive for the Na-K-2Cl cotransporter. After 6 wk of age, 15-PGDH protein was found in the granules in subsets of the proximal tubules. Overall, these results support a possibility that 15-PGDH may regulate postnatal kidney development through interaction with COX-2.
authors.affiliation: Department of Internal Medicine, University of Utah and Veterans Affairs Medical Center, Salt Lke City, Utah;
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Internal Medicine, University of Utah and Veterans Affairs Medical Center, Salt Lke City, Utah;
authors.forename: Zhanjun
authors.initials: Z
authors.lastname: Jia
authors.affiliation: Department of Internal Medicine, University of Utah and Veterans Affairs Medical Center, Salt Lke City, Utah;
authors.forename: Ying
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Institute of Hypertension, Sun Yat-sen University School of Medicine, Guangzhou, China;
authors.forename: Li
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Department of Internal Medicine, University of Utah and Veterans Affairs Medical Center, Salt Lke City, Utah;
authors.forename: Maicy
authors.initials: M
authors.lastname: Downton
authors.affiliation: Guangdong Provincial People's Hospital and Guangdong Academy of Medical Sciences, Guangzhou, China; and.
authors.forename: Ren
authors.initials: R
authors.lastname: Chen
authors.affiliation: Department of Nephrology, Nanjing Children's Hospital, Affiliated to Nanjing Medical University, Nanjing, China.
authors.forename: Aihua
authors.initials: A
authors.lastname: Zhang
authors.affiliation: Department of Internal Medicine, University of Utah and Veterans Affairs Medical Center, Salt Lke City, Utah; Institute of Hypertension, Sun Yat-sen University School of Medicine, Guangzhou, China; Tianxin.Yang@hsc.utah.edu.
authors.forename: Tianxin
authors.initials: T
authors.lastname: Yang
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D051546:Cyclooxygenase 2 / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D006911:Hydroxyprostaglandin Dehydrogenases / Q000096:biosynthesis*; D007668:Kidney / Q000201:enzymology / Q000254:growth & development*; D007687:Kidney Tubules, Proximal / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar
chemical_list: D006911:Hydroxyprostaglandin Dehydrogenases; C035859:15-hydroxyprostaglandin dehydrogenase; D051546:Cyclooxygenase 2
keywords: 15-PDGH; COX-2; postnatal kidney development; prostaglandin; proximal tubule