title: Wnt/β-catenin signaling pathway and lipolysis enzymes participate in methylprednisolone induced fat differential distribution between subcutaneous and visceral adipose tissue.
abstract: Glucocorticoids (GCs) are well known to induce fat distribution, which is consistent with the central adiposity phenotype seen in Cushing's syndrome. GCs have been proposed to be both adipogenic and lipolytic in action within adipose tissues. Different adipogenic and lipolytic effects between subcutaneous adipose tissue (SAT) and visceral adipose tissue (VAT) are likely to play a role in GCs induced fat differential distribution. Wnt/β-catenin signaling pathway is one of the most important regulators in adipogenesis. Adipose triglyceride lipase (ATGL) and hormone sensitive lipase (HSL) are the major lipases contributing to lipolysis. In the present study, we measured fat depot masses and the expression of Wnt/β-catenin signaling pathway and lipolytic enzymes of female Sprague-Dawley rats treated with or without methylprednisolone. We assessed the roles of Wnt/β-catenin signaling pathway and lipolytic enzymes in fat differential distribution between SAT and VAT. Our data suggested that methylprednisolone could inhibit Wnt/β-catenin signaling pathway in SAT and VAT, increase the expression of ATGL and HSL in SAT, and decrease the expression of ATGL and HSL in VAT. The differential expression of lipolysis enzymes induced by methylprednisolone between SAT and VAT might play a crucial role in fat distribution. Those findings would offer novel insights into the mechanisms of GCs induced fat distribution.
authors.affiliation: Department of Metabolism and Endocrinology, The First Affiliated Hospital of University of South China, Hengyang, Hunan 421000, PR China.
authors.forename: Xinhua
authors.initials: X
authors.lastname: Xiao
authors.affiliation: Department of Metabolism and Endocrinology, The First Affiliated Hospital of University of South China, Hengyang, Hunan 421000, PR China.
authors.forename: Han
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Metabolism and Endocrinology, The First Affiliated Hospital of University of South China, Hengyang, Hunan 421000, PR China.
authors.forename: Jiaojiao
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Metabolism and Endocrinology, The First Affiliated Hospital of University of South China, Hengyang, Hunan 421000, PR China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Qi
authors.affiliation: Institute of Clinical Medicine, The First Affiliated Hospital of University of South China, Hengyang, Hunan 421000, PR China.
authors.forename: Xuyu
authors.initials: X
authors.lastname: Zu
authors.affiliation: Department of Metabolism and Endocrinology, The First Affiliated Hospital of University of South China, Hengyang, Hunan 421000, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.affiliation: Institute of Clinical Medicine, The First Affiliated Hospital of University of South China, Hengyang, Hunan 421000, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhong
authors.affiliation: Department of Metabolism and Endocrinology, The First Affiliated Hospital of University of South China, Hengyang, Hunan 421000, PR China.
authors.forename: Renxian
authors.initials: R
authors.lastname: Cao
authors.affiliation: Department of Metabolism and Endocrinology, The First Affiliated Hospital of University of South China, Hengyang, Hunan 421000, PR China.
authors.forename: Jianghua
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Metabolism and Endocrinology, The First Affiliated Hospital of University of South China, Hengyang, Hunan 421000, PR China. Electronic address: wen_gb@hotmail.com.
authors.forename: Gebo
authors.initials: G
authors.lastname: Wen
mesh_terms: D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D005260:Female; D050152:Intra-Abdominal Fat / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D008049:Lipase / Q000378:metabolism*; D008066:Lipolysis; D008775:Methylprednisolone / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D050151:Subcutaneous Fat / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D051153:Wnt Proteins / Q000378:metabolism*; D051176:beta Catenin / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D051153:Wnt Proteins; D051176:beta Catenin; D008049:Lipase; D008775:Methylprednisolone
keywords: Adipogenesis; Lipolysis; Methylprednisolone; Subcutaneous adipose tissue; Visceral adipose tissue