title: Sulforaphane inhibits the proliferation of the BIU87 bladder cancer cell line via IGFBP-3 elevation.
abstract: OBJECTIVE
  To investigate effects of sulforaphane on the BIU87 cell line and underlying mechanisms involving IGFBP-3.
  
  
  METHODS
  Both BIU87 and IGFBP-3-silenced BIU87 cells were treated with sulforaphane. Cell proliferation was detected by MTT assay. Cell cycle and apoptosis were determined via flow cytometry. Quantitative polymerase chain reaction and Western blotting were applied to analyze the expression of IGFBP-3 and NF-κB at both mRNA and protein levels.
  
  
  RESULTS
  Sulforaphane (80 μM) treatment could inhibit cell proliferation, inducing apoptosis and cell cycle arrest at G2/M phase. All these effects could be antagonized by IGFBP-3 silencing. Furthermore, sulforaphane (80 μM) could down-regulate NF-κB expression while elevating that of IGFBP-3.
  
  
  CONCLUSIONS
  Sulforaphane could suppress the proliferation of BIU87 cells via enhancing IGFBP-3 expression, which negatively regulating the NF-κB signaling pathway.
authors.affiliation: First Clinical Medical College of Lanzhou University, Lanzhou University, Lanzhou, China E-mail : chenyr_lzu@163.com.
authors.forename: Ya-Mei
authors.initials: YM
authors.lastname: Dang
authors.forename: Gang
authors.initials: G
authors.lastname: Huang
authors.forename: Yi-Rong
authors.initials: YR
authors.lastname: Chen
authors.forename: Zhong-Feng
authors.initials: ZF
authors.lastname: Dang
authors.forename: Cheng
authors.initials: C
authors.lastname: Chen
authors.forename: Feng-Lei
authors.initials: FL
authors.lastname: Liu
authors.forename: Ying-Fang
authors.initials: YF
authors.lastname: Guo
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Xie
mesh_terms: D016588:Anticarcinogenic Agents / Q000494:pharmacology; D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D006801:Humans; D018972:Insulin-Like Growth Factor Binding Protein 3 / Q000096:biosynthesis* / Q000235:genetics; D017879:Isothiocyanates / Q000494:pharmacology*; D059566:M Phase Cell Cycle Checkpoints / Q000187:drug effects; D016328:NF-kappa B / Q000096:biosynthesis / Q000235:genetics; D034622:RNA Interference; D012333:RNA, Messenger / Q000096:biosynthesis; D034741:RNA, Small Interfering; D015398:Signal Transduction / Q000187:drug effects; D013454:Sulfoxides; D001749:Urinary Bladder Neoplasms / Q000188:drug therapy*
chemical_list: D016588:Anticarcinogenic Agents; C515497:IGFBP3 protein, human; D018972:Insulin-Like Growth Factor Binding Protein 3; D017879:Isothiocyanates; D016328:NF-kappa B; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D013454:Sulfoxides; C016766:sulforaphane