title: A pilot-scale homogenization-assisted negative pressure cavitation extraction of Astragalus polysaccharides.
abstract: This paper reported a new, green and effective extraction technique for polysaccharides, namely homogenization-assisted negative pressure cavitation extraction (HNPCE), which succeeded in the extraction of Astragalus polysaccharides (APs). Central composite design and kinetic model were applied to optimize the extraction conditions, and the optimal parameters were obtained as follows: homogenization time 70s, negative pressure -0.068MPa, extraction temperature 64.8°C, ratio of water to material 13.4 and extraction time 53min. The proposed method exhibited considerable predominance in terms of higher APs yield (16.74%) with much lower temperature and shorter duration, as against the reported hot water extraction method (14.33% of APs yield with 100°C and 3h). Moreover, FT-IR results showed that HNPCE method did not alter the primary structure of polysaccharides. Furthermore, the pilot-scale application of HNPCE was successfully performed with 16.62% of APs yield. Thus, HNPCE is an excellent alternative method for the extraction of polysaccharides from Astragalus or other plant materials in industry.
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, PR China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Jiao
authors.affiliation: State Engineering Laboratory of Bio-Resource Eco-Utilization, Northeast Forestry University, Harbin 150040, PR China.
authors.forename: Fu-Yao
authors.initials: FY
authors.lastname: Wei
authors.affiliation: State Engineering Laboratory of Bio-Resource Eco-Utilization, Northeast Forestry University, Harbin 150040, PR China.
authors.forename: Qing-Yan
authors.initials: QY
authors.lastname: Gai
authors.affiliation: State Engineering Laboratory of Bio-Resource Eco-Utilization, Northeast Forestry University, Harbin 150040, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: State Engineering Laboratory of Bio-Resource Eco-Utilization, Northeast Forestry University, Harbin 150040, PR China.
authors.forename: Meng
authors.initials: M
authors.lastname: Luo
authors.affiliation: State Engineering Laboratory of Bio-Resource Eco-Utilization, Northeast Forestry University, Harbin 150040, PR China. Electronic address: yujie_fu2011@yahoo.com.
authors.forename: Yu-Jie
authors.initials: YJ
authors.lastname: Fu
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, PR China; School of Pharmaceutical, Heilongjiang University of Chinese Medicine, Harbin 150040, PR China. Electronic address: mawei@hljucm.net.
authors.forename: Wei
authors.initials: W
authors.lastname: Ma
mesh_terms: D027883:Astragalus Plant / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification; D011312:Pressure; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D014867:Water / Q000737:chemistry
chemical_list: D011134:Polysaccharides; D014867:Water
keywords: Astragalus polysaccharides; Negative pressure cavitation extraction; Pilot-scale application