title: Crystallization and preliminary X-ray analysis of a highly stable novel SGNH hydrolase (Est24) from Sinorhizobium meliloti.
abstract: The SGNH hydrolase family includes enzymes that catalyze the hydrolysis of a broad range of substrates. Here, the crystallization and preliminary X-ray crystallographic studies of a novel SGNH hydrolase (Est24) from Sinorhizobium meliloti were performed. Recombinant Est24 protein containing an N-terminal His tag was expressed in Escherichia coli and purified to homogeneity. Est24 was then crystallized using a solution consisting of 0.2 M ammonium phosphate pH 4.6, 20% polyethylene glycol 3350. X-ray diffraction data were collected to a resolution of 1.45 Å with an R(merge) of 9.4%. The Est24 crystals belonged to space group C2, with unit-cell parameters a = 129.09, b = 88.63, c = 86.15 Å, α = 90.00, β = 114.30, γ = 90.00°. A molecular-replacement solution was obtained using the crystal structure of Mycobacterium smegmatis arylesterase as a template and structure refinement of Est24 is in progress.
authors.affiliation: Department of Applied Chemistry and Biological Engineering, College of Engineering, Ajou University, Suwon 443-749, Republic of Korea.
authors.forename: Bum Han
authors.initials: BH
authors.lastname: Ryu
authors.affiliation: Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon 440-746, Republic of Korea.
authors.forename: Duy Duc
authors.initials: DD
authors.lastname: Nguyen
authors.affiliation: Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon 440-746, Republic of Korea.
authors.forename: Tri Duc
authors.initials: TD
authors.lastname: Ngo
authors.affiliation: Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon 440-746, Republic of Korea.
authors.forename: Changsuk
authors.initials: C
authors.lastname: Oh
authors.affiliation: Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon 440-746, Republic of Korea.
authors.forename: Ramesh
authors.initials: R
authors.lastname: Pandian
authors.affiliation: Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon 440-746, Republic of Korea.
authors.forename: Kyeong Kyu
authors.initials: KK
authors.lastname: Kim
authors.affiliation: Department of Applied Chemistry and Biological Engineering, College of Engineering, Ajou University, Suwon 443-749, Republic of Korea.
authors.forename: T Doohun
authors.initials: TD
authors.lastname: Kim
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods*; D004795:Enzyme Stability; D006867:Hydrolases / Q000737:chemistry*; D011487:Protein Conformation; D016962:Sinorhizobium meliloti / Q000201:enzymology*
chemical_list: D006867:Hydrolases
keywords: Est24; SGNH hydrolase; Sinorhizobium meliloti