title: A preliminary study for identification of candidate AFLP markers under artificial selection for shell color in pearl oyster Pinctada fucata.
abstract: Pearl oyster Pinctada fucata is widely cultured to produce seawater pearl in South China, and the quality of pearl is significantly affected by its shell color. Thus the Pearl Oyster Selective Breeding Program (POSBP) was carried out for the shell color and growth traits. The black (B), gold (G), red (R) and white (W) shell strains with fast growth trait were achieved after five successive generation selection. In this study, AFLP technique was used to scan genome of four strains with different shell colors to identify the candidate markers under artificial selection. Eight AFLP primer combinations were screened and yielded 688 loci, 676 (98.26%) of which were polymorphic. In black, gold, red and white strains, the percentage of polymorphic loci was 90.41%, 87.79%, 93.60% and 93.31%, respectively, Nei's gene diversity was 0.3225, 0.2829, 0.3221 and 0.3292, Shannon's information index was 0.4801, 0.4271, 0.4825 and 0.4923, and the value of FST was 0.1805. These results suggested that the four different shell color strains had high genetic diversity and great genetic differentiation among strains, which had been subjected to the continuous selective pressures during the artificial selective breeding. Furthermore, six outlier loci were considered as the candidate markers under artificial selection for shell color. This study provides a molecular evidence for the inheritance of shell color of P. fucata.
authors.affiliation: Division of Aquaculture and Biotechnology, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou 510300, China; School of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China.
authors.forename: Keshu
authors.initials: K
authors.lastname: Zou
authors.affiliation: Division of Aquaculture and Biotechnology, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou 510300, China; Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture, Guangzhou 510300, China. Electronic address: zhangdch@163.com.
authors.forename: Dianchang
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Division of Aquaculture and Biotechnology, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou 510300, China.
authors.forename: Huayang
authors.initials: H
authors.lastname: Guo
authors.affiliation: Division of Aquaculture and Biotechnology, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou 510300, China.
authors.forename: Caiyan
authors.initials: C
authors.lastname: Zhu
authors.affiliation: Division of Aquaculture and Biotechnology, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou 510300, China; Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture, Guangzhou 510300, China.
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.affiliation: Division of Aquaculture and Biotechnology, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou 510300, China; Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture, Guangzhou 510300, China. Electronic address: jiangsg@21cn.com.
authors.forename: Shigui
authors.initials: S
authors.lastname: Jiang
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis; D000818:Animals; D001947:Breeding; D005819:Genetic Markers / Q000235:genetics; D014644:Genetic Variation; D010858:Pigmentation / Q000235:genetics*; D049911:Pinctada / Q000235:genetics*; D012641:Selection, Genetic
chemical_list: D005819:Genetic Markers
keywords: AFLP; Artificial selection; Outlier loci; Pinctada fucata; Shell color