title: P-selectin-mediated platelet adhesion promotes the metastasis of murine melanoma cells.
abstract: Studies have indicated that the aggregation of activated platelets with cancer cells facilitates tumor metastasis; the adhesion molecule P-selectin may be an important mediator of this process, but the detailed mechanism is unclear. In the current study, we established a B16F10 (B16) cell metastatic model in P-selectin knockout (P-sel-/-) mice to determine the effect of P-selectin-mediated platelet adhesion on metastasis. Compared with C57 mice, P-sel-/- mice developed fewer metastatic foci, and cell proliferation within the metastatic tumors was inhibited by P-selectin deficiency. The platelet refusion assay demonstrated that mice with P-sel-/- platelets developed fewer lung metastatic foci (P<0.01) with a lower microvascular density (MVD) than mice with wild-type platelets. A co-culture model of platelets and B16 cells was utilized to determine the difference in VEGF concentration in the supernatants. The results demonstrated that the supernatant from the P-sel-/- platelet/B16 co-culture had a lower concentration of VEGF. Therefore, our findings indicated that P-selectin deficiency inhibited the metastasis of B16 cells and that wild-type platelet refusion reversed this inhibition. The P-selectin-mediated interaction between platelets and B16 cells promoted angiogenesis by up-regulating VEGF.
authors.affiliation: Vascular Biology Research Institute, Guangdong Pharmaceutical University, Guangzhou, China.
authors.forename: Cui-Ling
authors.initials: CL
authors.lastname: Qi
authors.affiliation: Department of Gastrointestinal Surgery, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Wei
authors.affiliation: Vascular Biology Research Institute, Guangdong Pharmaceutical University, Guangzhou, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Ye
authors.affiliation: Vascular Biology Research Institute, Guangdong Pharmaceutical University, Guangzhou, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Vascular Biology Research Institute, Guangdong Pharmaceutical University, Guangzhou, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.affiliation: Vascular Biology Research Institute, Guangdong Pharmaceutical University, Guangzhou, China.
authors.forename: Qian-Qian
authors.initials: QQ
authors.lastname: Zhang
authors.affiliation: Vascular Biology Research Institute, Guangdong Pharmaceutical University, Guangzhou, China.
authors.forename: Jiang-Chao
authors.initials: JC
authors.lastname: Li
authors.affiliation: Vascular Biology Research Institute, Guangdong Pharmaceutical University, Guangzhou, China.
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: He
authors.affiliation: Vascular Biology Research Institute, Guangdong Pharmaceutical University, Guangzhou, China.
authors.forename: Tian
authors.initials: T
authors.lastname: Lan
authors.affiliation: Vascular Biology Research Institute, Guangdong Pharmaceutical University, Guangzhou, China.
authors.forename: Li-Jing
authors.initials: LJ
authors.lastname: Wang
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D004195:Disease Models, Animal; D008546:Melanoma, Experimental / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D051379:Mice; D018345:Mice, Knockout; D009362:Neoplasm Metastasis; D009389:Neovascularization, Pathologic / Q000235:genetics / Q000378:metabolism; D019007:P-Selectin / Q000235:genetics / Q000378:metabolism*; D010973:Platelet Adhesiveness / Q000235:genetics; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism
chemical_list: D019007:P-Selectin; D042461:Vascular Endothelial Growth Factor A