title: Efficient glycosylation of puerarin by an organic solvent-tolerant strain of Lysinibacillus fusiformis.
abstract: A bacterial strain able to glycosylate the plant natural product puerarin was isolated from local soil in Nanjing, China. It was identified as Lysinibacillus fusiformis, and deposited in China General Microbiological Culture Collection (CGMCC) under accession number 4913. Incubation of this strain with puerarin led to efficient production (91.6% conversation rate) of puerarin-7-O-fructoside, a derivative that possesses improved water solubility and antioxidant activity. A minor product puerarin-7-O-isomaltoside was also produced in small amounts, with a conversion rate of less than 1% after 48-h reaction. Both products were characterized based on the spectral data. Among the four tested sugars, sucrose (92.6% conversion rate of puerarin) is the best glycosyl donor for L. fusiformis CGMCC 4913, followed by maltose (39.8% conversion rate of puerarin), while glucose and fructose are not appropriate donors for this biotransformation process. L. fusiformis CGMCC 4913 can survive in the presence of 10% (v/v) organic solvents such as methanol, ethanol, toluene, cyclohexane, and dimethyl sulfoxide. The biotransformation efficiency of puerarin was increased 2-fold in the presence of 10% ethanol at 12 h compared to the transformation solution without ethanol. The optimum pH and substrate concentration are 8.0 and 4 g/L, respectively. Under the optimal conditions, the final conversion rate of puerarin reached 97.6±2.3% at 48 h in the presence of 10% ethanol. Therefore, L. fusiformis CGMCC 4913 represents a new and efficient biocatalyst for the biotransformation of puerarin.
authors.affiliation: Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, College of Life Science, Nanjing Normal University, 1 Wenyuan Road, Nanjing, Jiangsu 210046, China; Department of Biological Engineering, Utah State University, 4105 Old Main Hill, Logan, UT 84322-4105, USA.
authors.forename: Siyuan
authors.initials: S
authors.lastname: Wang
authors.affiliation: Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, College of Life Science, Nanjing Normal University, 1 Wenyuan Road, Nanjing, Jiangsu 210046, China. Electronic address: lgy@jsie.edu.cn.
authors.forename: Guiyou
authors.initials: G
authors.lastname: Liu
authors.affiliation: Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, College of Life Science, Nanjing Normal University, 1 Wenyuan Road, Nanjing, Jiangsu 210046, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, College of Life Science, Nanjing Normal University, 1 Wenyuan Road, Nanjing, Jiangsu 210046, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Cai
authors.affiliation: Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, College of Life Science, Nanjing Normal University, 1 Wenyuan Road, Nanjing, Jiangsu 210046, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Cheng
authors.affiliation: Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, College of Life Science, Nanjing Normal University, 1 Wenyuan Road, Nanjing, Jiangsu 210046, China.
authors.forename: Yin
authors.initials: Y
authors.lastname: Ji
authors.affiliation: Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, College of Life Science, Nanjing Normal University, 1 Wenyuan Road, Nanjing, Jiangsu 210046, China; Department of Biological Engineering, Utah State University, 4105 Old Main Hill, Logan, UT 84322-4105, USA.
authors.forename: Lei
authors.initials: L
authors.lastname: Sun
authors.affiliation: Department of Biological Engineering, Utah State University, 4105 Old Main Hill, Logan, UT 84322-4105, USA. Electronic address: jixun.zhan@usu.edu.
authors.forename: Jixun
authors.initials: J
authors.lastname: Zhan
authors.affiliation: Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, College of Life Science, Nanjing Normal University, 1 Wenyuan Road, Nanjing, Jiangsu 210046, China. Electronic address: yuansheng@njnu.edu.cn.
authors.forename: Sheng
authors.initials: S
authors.lastname: Yuan
mesh_terms: D001406:Bacillaceae / Q000302:isolation & purification / Q000378:metabolism* / Q000648:ultrastructure; D001711:Biotransformation; D002681:China; D000431:Ethanol; D005632:Fructose / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism; D006031:Glycosylation; D007529:Isoflavones / Q000737:chemistry / Q000378:metabolism*; D046529:Microscopy, Electron, Transmission; D009930:Organic Chemicals; D012988:Soil Microbiology; D012995:Solubility; D012997:Solvents; D014665:Vasodilator Agents / Q000737:chemistry / Q000378:metabolism
chemical_list: D007529:Isoflavones; D009930:Organic Chemicals; D012997:Solvents; D014665:Vasodilator Agents; C568770:puerarin-7-O-fructoside; D005632:Fructose; D000431:Ethanol; C033607:puerarin
keywords: Biotransformation; Lysinibacillus fusiformis; Organic solvent-tolerant; Puerarin; Puerarin-7-O-fructoside