title: An electrochemical and surface plasmon resonance study of adsorption actions of DNA by Escherichia coli.
abstract: In this work, we proposed an immobilization scheme targeting Escherichia coli bacterial cells onto gold surface utilizing polypeptide RGD's binding specificity to gold particles. Adsorption kinetics of extracellular Herring Sperm DNA on E. coli was then studied using electrochemical methods along with surface plasmon resonance spectroscopy through this immobilization scheme. The adsorption equilibrium constants of DNA adsorbing to E. coli from electrochemical method and surface plasmon resonance spectroscopy, were (5.596±0.462)×105 L mol(-1) and (1.24±0.361)×105 L mol(-1), accordingly. Importantly, this is the first study that used an electrochemical method to express the adsorptive action of DNA by E. coli.
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan 430072, PR China.
authors.forename: Juan
authors.initials: J
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Virology & Key Laboratory of Analytical Chemistry for Biology and Medicine, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, PR China; Center of Technology, China Tobacco Hubei Industrial LLC, Wuhan 430040, PR China.
authors.forename: Ran
authors.initials: R
authors.lastname: Li
authors.affiliation: State Key Laboratory of Virology & Key Laboratory of Analytical Chemistry for Biology and Medicine, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, PR China.
authors.forename: Feng-Lei
authors.initials: FL
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of Virology & Key Laboratory of Analytical Chemistry for Biology and Medicine, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, PR China; Center of Technology, Hongyunhonghe Tobacco (Group) Corporation, Kunming 650231, PR China.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Virology & Key Laboratory of Analytical Chemistry for Biology and Medicine, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, PR China.
authors.forename: Qing-Ying
authors.initials: QY
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Virology & Key Laboratory of Analytical Chemistry for Biology and Medicine, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, PR China.
authors.forename: Qiu-Li-Yang
authors.initials: QL
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Virology & Key Laboratory of Analytical Chemistry for Biology and Medicine, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, PR China; College of Chemistry and Material Sciences, South-Central University for Nationalities, Wuhan 430072, PR China.
authors.forename: Xiao-Le
authors.initials: XL
authors.lastname: Han
authors.affiliation: State Key Laboratory of Virology & Key Laboratory of Analytical Chemistry for Biology and Medicine, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, PR China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Lin
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan 430072, PR China. Electronic address: wang200631550010@126.com.
authors.forename: Hong
authors.initials: H
authors.lastname: He
authors.affiliation: State Key Laboratory of Virology & Key Laboratory of Analytical Chemistry for Biology and Medicine, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, PR China; Department of Chemistry and Life Sciences, Xianning University, Xianning 437005, PR China. Electronic address: yiliuchem@whu.edu.cn.
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan 430072, PR China.
authors.forename: Yun-Long
authors.initials: YL
authors.lastname: Wang
mesh_terms: D000327:Adsorption; D000818:Animals; D004247:DNA / Q000378:metabolism*; D058266:Dielectric Spectroscopy; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D004926:Escherichia coli / Q000378:metabolism*; D005399:Fishes; D006046:Gold / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008297:Male; D018625:Microscopy, Atomic Force; D013094:Spermatozoa / Q000378:metabolism; D020349:Surface Plasmon Resonance; D013499:Surface Properties
chemical_list: D006046:Gold; D004247:DNA
keywords: Adsorption; DNA; Electrochemistry; Escherichia coli; Surface Plasmon Resonance