title: WOX11 and 12 are involved in the first-step cell fate transition during de novo root organogenesis in Arabidopsis.
abstract: De novo organogenesis is a process through which wounded or detached plant tissues or organs regenerate adventitious roots and shoots. Plant hormones play key roles in de novo organogenesis, whereas the mechanism by which hormonal actions result in the first-step cell fate transition in the whole process is unknown. Using leaf explants of Arabidopsis thaliana, we show that the homeobox genes WUSCHEL RELATED HOMEOBOX11 (WOX11) and WOX12 are involved in de novo root organogenesis. WOX11 directly responds to a wounding-induced auxin maximum in and surrounding the procambium and acts redundantly with its homolog WOX12 to upregulate LATERAL ORGAN BOUNDARIES DOMAIN16 (LBD16) and LBD29, resulting in the first-step cell fate transition from a leaf procambium or its nearby parenchyma cell to a root founder cell. In addition, our results suggest that de novo root organogenesis and callus formation share a similar mechanism at initiation.
authors.affiliation: National Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China.
authors.forename: Jingchun
authors.initials: J
authors.lastname: Liu
authors.forename: Lihong
authors.initials: L
authors.lastname: Sheng
authors.forename: Yingqiang
authors.initials: Y
authors.lastname: Xu
authors.forename: Jiqin
authors.initials: J
authors.lastname: Li
authors.forename: Zhongnan
authors.initials: Z
authors.lastname: Yang
authors.forename: Hai
authors.initials: H
authors.lastname: Huang
authors.forename: Lin
authors.initials: L
authors.lastname: Xu
mesh_terms: D017360:Arabidopsis / Q000166:cytology* / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000235:genetics / Q000502:physiology*; D019070:Cell Lineage; D005801:Genes, Homeobox; D017343:Genes, Plant; D063246:Organogenesis, Plant / Q000502:physiology*; D018517:Plant Roots / Q000166:cytology* / Q000254:growth & development
chemical_list: D029681:Arabidopsis Proteins