title: The miR-29b-Sirt1 axis regulates self-renewal of mouse embryonic stem cells in response to reactive oxygen species.
abstract: Endogenous reactive oxygen species (ROS) control is important for the maintenance of self-renewal of embryonic stem (ES) cells. Although miRNAs have been found to be critically involved in the regulation of the self-renewal, whether miRNAs can regulate the signaling axis to control ROS in ES cells is unclear. Here we show that miR-29b specifically regulates the self-renewal of mouse ES cells in response to ROS generated by antioxidant-free culture. Sirt1 is the direct target of miR-29b and can also make mES cells sensitive to ROS and regulate the self-renewal of mES cells during the response of ROS. We further found that Sirt1 could attenuate the miR-29b function in regulating mES cells' self-renewal in response to ROS. Our results determined that miR-29b-Sirt1 axis regulates self-renewal of mES cells in response to ROS.
authors.affiliation: Research Center for Translational Medicine, East Hospital, Tongji University School of Medicine, Shanghai 200120, People's Republic of China; Department of Preventive Medicine, Tongji University School of Medicine, East Hospital, Shanghai 200120, People's Republic of China.
authors.forename: Zengguang
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Research Center for Translational Medicine, East Hospital, Tongji University School of Medicine, Shanghai 200120, People's Republic of China; Department of Anesthesiology, East Hospital, Tongji University School of Medicine, 150 Jimo Road, Shanghai 200120, People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Hospital Infections, Shanghai Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, People's Republic of China.
authors.forename: Xuejie
authors.initials: X
authors.lastname: Fei
authors.affiliation: Department of Anesthesiology, The Eye, Ear, Nose, and Throat Hospital, Fudan University, Shanghai 200031, People's Republic of China.
authors.forename: Xiuwen
authors.initials: X
authors.lastname: Yi
authors.affiliation: Department of Anesthesiology, The Eye, Ear, Nose, and Throat Hospital, Fudan University, Shanghai 200031, People's Republic of China. Electronic address: wenxianli66@gmail.com.
authors.forename: Wenxian
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Anesthesiology, East Hospital, Tongji University School of Medicine, 150 Jimo Road, Shanghai 200120, People's Republic of China. Electronic address: xuzg1998@126.com.
authors.forename: Qingxiu
authors.initials: Q
authors.lastname: Wang
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D049109:Cell Proliferation; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000166:cytology*; D018398:Homeodomain Proteins / Q000096:biosynthesis; D008623:Mercaptoethanol / Q000494:pharmacology; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D000071317:Nanog Homeobox Protein; D050814:Octamer Transcription Factor-3 / Q000096:biosynthesis; D034622:RNA Interference; D034741:RNA, Small Interfering; D017382:Reactive Oxygen Species / Q000378:metabolism*; D055748:SOXB1 Transcription Factors / Q000096:biosynthesis; D015398:Signal Transduction / Q000235:genetics; D056564:Sirtuin 1 / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D020413:3' Untranslated Regions; D018398:Homeodomain Proteins; C547014:MIRN29 microRNA, mouse; D035683:MicroRNAs; D000071317:Nanog Homeobox Protein; C475540:Nanog protein, mouse; D050814:Octamer Transcription Factor-3; C494174:Pou5f1 protein, mouse; D034741:RNA, Small Interfering; D017382:Reactive Oxygen Species; D055748:SOXB1 Transcription Factors; C527783:Sox2 protein, mouse; D008623:Mercaptoethanol; C470545:Sirt1 protein, mouse; D056564:Sirtuin 1
keywords: Mouse embryonic stem cell; Reactive oxygen species; Self-renewal; Sirt1; miR-29b