title: Detection of Coconut cadang-cadang viroid (CCCVd) in oil palm by reverse transcription loop-mediated isothermal amplification (RT-LAMP).
abstract: A reverse transcription loop-mediated isothermal amplification (RT-LAMP) detected Coconut cadang-cadang viroid (CCCVd) within 60 min at 60 °C in total nucleic acid extracted from oil palm leaves infected with CCCVd. Positive reactions showed colour change from orange to green in the reaction mix after the addition of fluorescent reagent, and a laddering pattern band on 2% agarose gel electrophoresis. Conventional RT-PCR with LAMP primers produced amplicons with a sequence identical to the 297-nt CCCVd oil palm variant with the primers being specific for CCCVd and not for other viroids such as PSTVd and CEVd. RT-LAMP was found to be rapid and specific for detecting oil palm CCCVd.
authors.affiliation: Laboratory of Molecular Biology, Institute of Tropical Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors.forename: Sathis Sri
authors.initials: SS
authors.lastname: Thanarajoo
authors.affiliation: Laboratory of Molecular Biology, Institute of Tropical Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors.forename: Lih Ling
authors.initials: LL
authors.lastname: Kong
authors.affiliation: Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors.forename: Jugah
authors.initials: J
authors.lastname: Kadir
authors.affiliation: Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors.forename: Wei Hongi
authors.initials: WH
authors.lastname: Lau
authors.affiliation: Laboratory of Molecular Biology, Institute of Tropical Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia; Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia. Electronic address: ganesanv@upm.edu.my.
authors.forename: Ganesan
authors.initials: G
authors.lastname: Vadamalai
mesh_terms: D000074263:Coconut Oil; D003059:Cocos / Q000821:virology*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000821:virology; D010938:Plant Oils / Q000302:isolation & purification; D048348:Reverse Transcription; D012680:Sensitivity and Specificity; D013696:Temperature; D013997:Time Factors; D014772:Viroids / Q000302:isolation & purification*
chemical_list: D010938:Plant Oils; D000074263:Coconut Oil
keywords: Coconut cadang-cadang viroid; Oil palm; RT-LAMP