title: Development of hydrogel biochip for in vitro allergy diagnostics.
abstract: A hydrogel biochip was developed for the simultaneous quantitative determination of sIgE for 21 allergens and total IgE in human serum. The biochips are manufactured by photoinduced copolymerization of different molecules (allergens and antibodies) with gel-forming monomers resulting in the formation of three-dimensional hydrogel elements (1nl gel drops). After incubation of the biochip with the serum, the results are visualized using fluorescently labeled anti-IgE antibodies. Using biochips, serum samples from allergic patients and healthy donors were analyzed and good correlation with the results obtained using commercial EIA test systems of generally recognized quality (Dr. Fooke Laboratorien GmbH, Germany) was observed.
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences (EIMB RAS), Moscow, Russia; Moscow Institute of Physics and Technology (State University), Moscow, Russia.
authors.forename: G U
authors.initials: GU
authors.lastname: Feyzkhanova
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences (EIMB RAS), Moscow, Russia.
authors.forename: M A
authors.initials: MA
authors.lastname: Filippova
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences (EIMB RAS), Moscow, Russia.
authors.forename: V O
authors.initials: VO
authors.lastname: Talibov
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences (EIMB RAS), Moscow, Russia.
authors.forename: E I
authors.initials: EI
authors.lastname: Dementieva
authors.affiliation: Federal State Budgetary Institution Polyclinic №1 of the Business Administration for the President of the Russian Federation, Moscow, Russia.
authors.forename: V V
authors.initials: VV
authors.lastname: Maslennikov
authors.affiliation: Federal State Budgetary Institution Polyclinic №1 of the Business Administration for the President of the Russian Federation, Moscow, Russia.
authors.forename: Yu P
authors.initials: YP
authors.lastname: Reznikov
authors.affiliation: Dr. Fooke Laboratorien GmbH, Neuss, Germany.
authors.forename: N
authors.initials: N
authors.lastname: Offermann
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences (EIMB RAS), Moscow, Russia; Moscow Institute of Physics and Technology (State University), Moscow, Russia.
authors.forename: A S
authors.initials: AS
authors.lastname: Zasedatelev
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences (EIMB RAS), Moscow, Russia. Electronic address: arubina@biochip.ru.
authors.forename: A Yu
authors.initials: AY
authors.lastname: Rubina
authors.affiliation: Dr. Fooke Laboratorien GmbH, Neuss, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Fooke-Achterrath
mesh_terms: D000485:Allergens / Q000276:immunology; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D000941:Antigens / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006801:Humans; D020100:Hydrogels / Q000138:chemical synthesis*; D006967:Hypersensitivity / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D007073:Immunoglobulin E / Q000097:blood* / Q000276:immunology; D040081:Protein Array Analysis / Q000379:methods*; D012882:Skin Tests
chemical_list: D000485:Allergens; D000888:Antibodies, Anti-Idiotypic; D000941:Antigens; D020100:Hydrogels; C026408:anti-IgE antibodies; D007073:Immunoglobulin E
keywords: Allergy diagnostics; Biochip; IgE in vitro test; Protein microarray