title: A novel perfused rotary bioreactor for cardiomyogenesis of embryonic stem cells.
abstract: Developments in bioprocessing technology play an important role for overcoming challenges in cardiac tissue engineering. To this end, our laboratory has developed a novel rotary perfused bioreactor for supporting three-dimensional cardiac tissue engineering. The dynamic culture environments provided by our novel perfused rotary bioreactor and/or the high-aspect rotating vessel produced constructs with higher viability and significantly higher cell numbers (up to 4 × 10(5) cells/bead) than static tissue culture flasks. Furthermore, cells in the perfused rotary bioreactor showed earlier gene expressions of cardiac troponin-T, α- and β-myosin heavy chains with higher percentages of cardiac troponin-I-positive cells and better uniformity of sacromeric α-actinin expression. A dynamic and perfused environment, as provided by this bioreactor, provides a superior culture performance in cardiac differentiation for embryonic stem cells particularly for larger 3D constructs.
authors.affiliation: School of Chemical & Biomedical Engineering, Nanyang Technological University, Singapore, Singapore, teoa0003@e.ntu.edu.sg.
authors.forename: Ailing
authors.initials: A
authors.lastname: Teo
authors.forename: Athanasios
authors.initials: A
authors.lastname: Mantalaris
authors.forename: Kedong
authors.initials: K
authors.lastname: Song
authors.forename: Mayasari
authors.initials: M
authors.lastname: Lim
mesh_terms: D000818:Animals; D019149:Bioreactors; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D002454:Cell Differentiation / Q000502:physiology*; D053595:Embryonic Stem Cells / Q000166:cytology*; D051379:Mice; D032383:Myocytes, Cardiac / Q000166:cytology*