title: Characterization of a full-length infectious clone of bovine foamy virus 3026.
abstract: The biological features of most foamy viruses (FVs) are poorly understood, including bovine foamy virus (BFV). BFV strain 3026 (BFV3026) was isolated from the peripheral blood mononuclear cells of an infected cow in Zhangjiakou, China. A full-length genomic clone of BFV3026 was obtained from BFV3026-infected cells, and it exhibited more than 99% amino acid (AA) homology to another BFV strain isolated in the USA. Upon transfection into fetal canine thymus cells, the full-length BFV3026 clone produced viral structural and auxiliary proteins, typical cytopathic effects, and virus particles. These results demonstrate that the full-length BFV3026 clone is fully infectious and can be used in further BFV3026 research.
authors.affiliation: Key Laboratory of Molecular Microbiology and Biotechnology (Ministry of Education) and Key Laboratory of Microbial Functional Genomics (Tianjin), College of Life Sciences, Nankai University, Tianjin, 300071, China.
authors.forename: Tiejun
authors.initials: T
authors.lastname: Bing
authors.forename: Hong
authors.initials: H
authors.lastname: Yu
authors.forename: Yue
authors.initials: Y
authors.lastname: Li
authors.forename: Lei
authors.initials: L
authors.lastname: Sun
authors.forename: Juan
authors.initials: J
authors.lastname: Tan
authors.forename: Yunqi
authors.initials: Y
authors.lastname: Geng
authors.forename: Wentao
authors.initials: W
authors.lastname: Qiao
mesh_terms: D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D002681:China; D003001:Cloning, Molecular; D003588:Cytopathogenic Effect, Viral; D007963:Leukocytes, Mononuclear / Q000821:virology; D012689:Sequence Homology, Nucleic Acid; D016092:Spumavirus / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification / Q000502:physiology*; D014764:Viral Proteins / Q000096:biosynthesis; D014771:Virion / Q000648:ultrastructure; D014779:Virus Replication
chemical_list: D014764:Viral Proteins