title: Anti-phosphorylated histone H2AThr120: a universal microscopic marker for centromeric chromatin of mono- and holocentric plant species.
abstract: Based on the analysis of 20 different monocot and eudicot species, we propose that the centromeric distribution of the phosphorylated histone H2AThr120 is evolutionary highly conserved across species with mono- and holocentric chromosomes. Therefore, antibodies recognizing the phosphorylated threonine 120 of the histone H2A can serve as a universal marker for the cytological detection of centromeres of mono- and holokinetic plant species. In addition, super resolution microscopy of signals specific to the centromere-specific histone H3 variant CENH3 and to H2AThr120ph revealed that these histone variants are incorporated into different nucleosomes, which form distinct, partly intermingled chromatin domains. This specific arrangement of both histone variants suggests different centromeric functions during the cell cycle.
authors.affiliation: Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany.
authors.forename: D
authors.initials: D
authors.lastname: Demidov
authors.forename: V
authors.initials: V
authors.lastname: Schubert
authors.forename: K
authors.initials: K
authors.lastname: Kumke
authors.forename: O
authors.initials: O
authors.lastname: Weiss
authors.forename: R
authors.initials: R
authors.lastname: Karimi-Ashtiyani
authors.forename: J
authors.initials: J
authors.lastname: Buttlar
authors.forename: S
authors.initials: S
authors.lastname: Heckmann
authors.forename: G
authors.initials: G
authors.lastname: Wanner
authors.forename: Q
authors.initials: Q
authors.lastname: Dong
authors.forename: F
authors.initials: F
authors.lastname: Han
authors.forename: A
authors.initials: A
authors.lastname: Houben
mesh_terms: D002503:Centromere / Q000235:genetics*; D002843:Chromatin / Q000235:genetics*; D032461:Chromosomes, Plant / Q000235:genetics*; D006657:Histones / Q000235:genetics* / Q000378:metabolism*; D009707:Nucleosomes / Q000235:genetics; D010766:Phosphorylation / Q000235:genetics*
chemical_list: D002843:Chromatin; D006657:Histones; D009707:Nucleosomes