title: Study of the effect of F17A mutation on characteristics of Bacillus thermocatenulatus lipase expressed in Pichia pastoris using in silico and experimental methods.
abstract: Bacillus thermocatenulatus lipase 2 (BTL2), a thermoalkalophilic lipase, is the best studied enzyme for its particular properties, which make it useful in different industries. Displacement of conserved phenylalanine 17 (Phe-17) residue in the active site of BTL2 has a critical role in oxyanion hole formation, which is important for enzyme activity. In this study, to facilitate oxyanion hole formation, Phe-17 was substituted with Alanine residue (F17A). The best structures of the opened form of the native and mutated lipases were garnered based on the crystal structures of 2W22. To evaluate catalytic activity, both lipases were docked to a set of ligands using Hex 6.3 software. Following in silico study, both native and mutant btl2 genes were cloned and expressed in Pichia pastoris. Based on the results obtained, the mutation increased lipase lipolytic activity against most of the applied substrates, especially for tributyrin and tricaprylin, by 1.9 and 2.15 fold, respectively. However, optimum temperature and pH were the same for both lipases (60 °C and pH 8.0). As previously reported, it is believed that F17A mutation simplifies oxyanion hole formation and declines steric hindrance in the enzyme active site, which might ultimately lead to more efficient accessibility of substrates.
authors.affiliation: Department of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
authors.forename: Esmat
authors.initials: E
authors.lastname: Karimi
authors.forename: Ali Asghar
authors.initials: AA
authors.lastname: Karkhane
authors.forename: Bagher
authors.initials: B
authors.lastname: Yakhchali
authors.forename: Mehdi
authors.initials: M
authors.lastname: Shamsara
authors.forename: Saeed
authors.initials: S
authors.lastname: Aminzadeh
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Torktaz
authors.forename: Mostafa
authors.initials: M
authors.lastname: Hosseini
authors.forename: Zahra
authors.initials: Z
authors.lastname: Safari
mesh_terms: D001407:Bacillus / Q000201:enzymology* / Q000235:genetics; D015870:Gene Expression / Q000235:genetics; D008049:Lipase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D062105:Molecular Docking Simulation; D009154:Mutation / Q000235:genetics*; D010649:Phenylalanine / Q000235:genetics*; D010843:Pichia / Q000235:genetics*
chemical_list: D010649:Phenylalanine; D008049:Lipase
keywords: Bacillus thermocatenulatus; docking; in silico; oxyanion hole