title: Choline in infant formula and adult/pediatric nutritional Formula by ultra high-performance liquid chromatography/ Tandem mass spectrometry: AOAC First Action 2012.18.
abstract: The method described below is for the determination of choline in infant formula and adult/pediatric nutritional formula by ultra high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS). The single-laboratory validation data were submitted to the Stakeholder Panel on Infant Formula and Adult Nutritionals (SPIFAN) Expert Review Panel (ERP) for review at the AOAC INTERNATIONAL Annual Meeting held September 30 to October 3, 2012 in Las Vegas, NV. The ERP determined that the data reviewed met the standard method performance requirements set by SPIFAN, and the method was approved as AOAC Official First Action. The analytical range was found to be between 0.16 and 3.2 microg/mL. The recovery rates were within 80-120% at 50 and 100% of native levels for all samples. Repeatability precision (RSDr) was < 3%, with intermediate reproducibility (RSDir) no higher than 4%.
authors.forename: Frederic
authors.initials: F
authors.lastname: Martin
authors.forename: Catherine
authors.initials: C
authors.lastname: Gimenez
authors.forename: Patric
authors.initials: P
authors.lastname: Fontannaz
authors.forename: Tamara
authors.initials: T
authors.lastname: Kilinc
authors.forename: Esther
authors.initials: E
authors.lastname: Campos-Giménez
authors.forename: Dawn
authors.initials: D
authors.lastname: Dowell
mesh_terms: D000328:Adult; D000818:Animals; D002138:Calibration; D002623:Chemistry Techniques, Analytical / Q000592:standards*; D002794:Choline / Q000032:analysis*; D002845:Chromatography / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005526:Food, Formulated / Q000032:analysis*; D006801:Humans; D006868:Hydrolysis; D007223:Infant; D041943:Infant Formula / Q000737:chemistry*; D008892:Milk; D012015:Reference Standards; D015203:Reproducibility of Results; D013025:Glycine max; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D002794:Choline