title: Nematode Sgt1-homologue D1054.3 binds open and closed conformations of Hsp90 via distinct binding sites.
abstract: Heat shock protein 90 (Hsp90) is a highly conserved ATP-driven machine involved in client protein maturation, folding, and activation. The chaperone is supported by a set of cochaperones that confer client specificities. One of those proteins is the suppressor of G2 allele of skp1 (Sgt1), which participates together with Hsp90 in the immune responses of plants. Sgt1 consists of three domains: a TPR-, CS-, and SGS-domain, conserved in plants, yeast, and humans. The TPR-domain though is lacking in nematodes and insects. We observe that the Caenorhabditis elegans Sgt1 homologue D1054.3 binds to Hsp90 in the absence of nucleotides but much stronger in the presence of ATP and ATPγS. The latter binding mode is similar to p23, another CS-domain containing Hsp90 cofactor, even though binding is not observable for p23 in the absence of nucleotides. We use point mutations in Hsp90, which accumulate different conformations in the ATPase cycle, to differentiate between binding to open and closed Hsp90 conformations. These data support a strong contribution of the Hsp90 conformation to Sgt1 binding and highlight the ability of this cofactor to interact with all known Hsp90 conformations albeit with different affinities.
authors.affiliation: Department of Chemistry, Technische Universität München , 85748 Garching, Germany.
authors.forename: Julia M
authors.initials: JM
authors.lastname: Eckl
authors.forename: Adrian
authors.initials: A
authors.lastname: Drazic
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Rutz
authors.forename: Klaus
authors.initials: K
authors.lastname: Richter
mesh_terms: D000818:Animals; D001665:Binding Sites; D017173:Caenorhabditis elegans / Q000378:metabolism; D029742:Caenorhabditis elegans Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005456:Fluorescent Dyes; D018841:HSP90 Heat-Shock Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017354:Point Mutation; D011487:Protein Conformation; D055550:Protein Stability
chemical_list: D029742:Caenorhabditis elegans Proteins; D005456:Fluorescent Dyes; D018841:HSP90 Heat-Shock Proteins