title: Importance of promoter methylation of GATA4 and TP53 genes in endometrioid carcinoma of endometrium.
abstract: BACKGROUND
  Epigenetic changes are considered to be a frequent event during tumor development. Various methylation changes have been identified and show promise as potential cancer biomarkers. The aim of this study was to investigate promoter methylation of GATA4 and TP53 genes in endometrioid carcinoma of endometrium.
  
  
  METHODS
  To search for promoter methylation of GATA4 and TP53 genes we used methylation-specific PCR (MSP) to compare the methylation status of 54 patients with endometrioid carcinoma of endometrium and 18 patients with normal endometrial tissue.
  
  
  RESULTS
  In our study MSP revealed GATA4 promoter methylation in 44 of 54 in the carcinoma group (81.5%), and in none of the control group. No methylation was observed in TP53 gene.
  
  
  CONCLUSIONS
  In conclusion, our study showed that there is significantly higher methylation in GATA4 gene in the endometrial cancer group compared with samples of non-neoplastic endometrium. The finding suggests the importance of hypermethylation of this gene in endometrial carcinogenesis and could have implications for future diagnostic and therapeutic strategies for endometrial cancer based on epigenetic changes.
authors.forename: Marcela
authors.initials: M
authors.lastname: Chmelarova
authors.forename: Spela
authors.initials: S
authors.lastname: Kos
authors.forename: Eva
authors.initials: E
authors.lastname: Dvorakova
authors.forename: Jiri
authors.initials: J
authors.lastname: Spacek
authors.forename: Jan
authors.initials: J
authors.lastname: Laco
authors.forename: Ema
authors.initials: E
authors.lastname: Ruszova
authors.forename: Katerina
authors.initials: K
authors.lastname: Hrochova
authors.forename: Vladimir
authors.initials: V
authors.lastname: Palicka
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018269:Carcinoma, Endometrioid / Q000235:genetics*; D016022:Case-Control Studies; D019175:DNA Methylation; D016889:Endometrial Neoplasms / Q000235:genetics*; D044127:Epigenesis, Genetic; D005260:Female; D050991:GATA4 Transcription Factor / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D016158:Genes, p53; D006801:Humans; D008875:Middle Aged; D011401:Promoter Regions, Genetic
chemical_list: D050991:GATA4 Transcription Factor; C484170:GATA4 protein, human