title: The novel miR-9500 regulates the proliferation and migration of human lung cancer cells by targeting Akt1.
abstract: MicroRNAs have crucial roles in lung cancer cell development. They regulate cell growth, proliferation and migration by mediating the expression of tumor suppressor genes and oncogenes. We identified and characterized the novel miR-9500 in human lung cancer cells. The miR-9500 forms a stem-loop structure and is conserved in other mammals. The expression levels of miR-9500 were reduced in lung cancer cells and lung cancer tissues compared with normal tissues, as verified by TaqMan miRNA assays. It was confirmed that the putative target gene, Akt1, was directly suppressed by miR-9500, as demonstrated by a luciferase reporter assay. The miR-9500 significantly repressed the protein expression levels of Akt1, as demonstrated via western blot, but did not affect the corresponding mRNA levels. Akt1 has an important role in lung carcinogenesis, and depletion of Akt1 has been shown to have antiproliferative and anti-migratory effects in previous studies. In the current study, the overexpression of miR-9500 inhibited cell proliferation and the expression of cell cycle-related proteins. Likewise, the overexpression of miR-9500 impeded cell migration in human lung cancer cells. In an in vivo assay, miR-9500 significantly suppressed Fluc expression compared with NC and ASO-miR-9500, suggesting that cell proliferation was inhibited in nude mice. Likewise, miR-9500 repressed tumorigenesis and metastasis by targeting Akt1. These data indicate that miR-9500 might be applicable for lung cancer therapy.
authors.affiliation: Department of Pharmacy, College of Pharmacy, CHA University, Yatap-dong, Bundang-gu, Seongnam-si, Gyeonggi-do, Republic of Korea.
authors.forename: J K
authors.initials: JK
authors.lastname: Yoo
authors.affiliation: Department of Pharmacy, College of Pharmacy, CHA University, Yatap-dong, Bundang-gu, Seongnam-si, Gyeonggi-do, Republic of Korea.
authors.forename: H Y
authors.initials: HY
authors.lastname: Jung
authors.affiliation: Department of Pharmacy, College of Pharmacy, CHA University, Yatap-dong, Bundang-gu, Seongnam-si, Gyeonggi-do, Republic of Korea.
authors.forename: J M
authors.initials: JM
authors.lastname: Lee
authors.affiliation: Department of Pharmacy, College of Pharmacy, CHA University, Yatap-dong, Bundang-gu, Seongnam-si, Gyeonggi-do, Republic of Korea.
authors.forename: H
authors.initials: H
authors.lastname: Yi
authors.affiliation: Department of Neurology, CHA Bundang Medical Center, CHA University, Seongnam-si, Gyeonggi-do, Republic of Korea.
authors.forename: S-H
authors.initials: SH
authors.lastname: Oh
authors.affiliation: Department of Biomedical Science, College of Life Science, CHA University, 605-21, Yeoksam1-dong, Gangnam-gu, Seoul, Republic of Korea.
authors.forename: H Y
authors.initials: HY
authors.lastname: Ko
authors.affiliation: Department of Pharmacy, College of Pharmacy, CHA University, Yatap-dong, Bundang-gu, Seongnam-si, Gyeonggi-do, Republic of Korea.
authors.forename: H
authors.initials: H
authors.lastname: Yoo
authors.affiliation: Department of Biomedical Science, College of Life Science, CHA University, 605-21, Yeoksam1-dong, Gangnam-gu, Seoul, Republic of Korea.
authors.forename: H-R
authors.initials: HR
authors.lastname: Kim
authors.affiliation: Department of Biomedical Science, College of Life Science, CHA University, 605-21, Yeoksam1-dong, Gangnam-gu, Seoul, Republic of Korea.
authors.forename: H
authors.initials: H
authors.lastname: Song
authors.affiliation: Department of Biomedical Science, College of Life Science, CHA University, 605-21, Yeoksam1-dong, Gangnam-gu, Seoul, Republic of Korea.
authors.forename: S
authors.initials: S
authors.lastname: Kim
authors.affiliation: Department of Pharmacy, College of Pharmacy, CHA University, Yatap-dong, Bundang-gu, Seongnam-si, Gyeonggi-do, Republic of Korea.
authors.forename: J K
authors.initials: JK
authors.lastname: Kim
mesh_terms: D020413:3' Untranslated Regions; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000201:enzymology / Q000235:genetics* / Q000473:pathology; D008297:Male; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000502:physiology*; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics* / Q000378:metabolism; D034622:RNA Interference
chemical_list: D020413:3' Untranslated Regions; C000600830:MIRN-9500 microRNA, human; D035683:MicroRNAs; C494918:AKT1 protein, human; D051057:Proto-Oncogene Proteins c-akt