title: Elevated expression of CD147 in patients with endometriosis and its role in regulating apoptosis and migration of human endometrial cells.
abstract: OBJECTIVE
  To examine the expression of CD147 in 60 human endometriosis lesions and how CD147 regulates migration and apoptosis in human uterine epithelial (HESs) cells.
  
  
  METHODS
  Experimental clinical study and laboratory-based investigation.
  
  
  METHODS
  Hospital and academic research center.
  
  
  METHODS
  Sixty women with chocolate cysts and 16 control women without endometriosis.
  
  
  METHODS
  Human uterine epithelial cells were treated with anti-CD147 antibody.
  
  
  METHODS
  Real-time polymerase chain reaction for detecting CD147 expression in 60 human endometriosis lesions; migration assay and CellTiter 96 AQueous One Solution Cell Proliferation Assay (MTS) assay for cell functional investigation; Western blot for detecting protein levels; gelatin zymography for evaluating the activity of matrix metalloproteinase-2 (MMP-2) in cultured cells.
  
  
  RESULTS
  Expression of CD147 was significantly higher in ectopic endometrial tissues from patients with endometriosis than in normal endometrial tissues. Interference with CD147 function led to decreased migration and cell viability in HESs cells. Surprisingly, MMP-2 expression and activity were not changed after treating HESs cells with anti-CD147 antibody. Further examination revealed that immunodepletion of CD147 induced apoptosis in HESs cells, leading to the activation of caspase 3 and poly(ADP-ribose) polymerase.
  
  
  CONCLUSIONS
  The results of the present study suggest that abnormally high expression of CD147 in ovarian endometriosis lesions with enhanced cell survival (reduced apoptosis) and migration, in an MMP-2-independent manner, may underlie the progression of endometriosis in humans.
authors.affiliation: Department of Gynecology, The Second People's Hospital of Shenzhen, Shenzhen, People's Republic of China.
authors.forename: Aihong
authors.initials: A
authors.lastname: Jin
authors.affiliation: Department of Gynecology, The Second People's Hospital of Shenzhen, Shenzhen, People's Republic of China; Epithelial Cell Biology Research Center, School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong.
authors.forename: Hao
authors.initials: H
authors.lastname: Chen
authors.affiliation: Epithelial Cell Biology Research Center, School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong.
authors.forename: Chaoqun
authors.initials: C
authors.lastname: Wang
authors.affiliation: Epithelial Cell Biology Research Center, School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong.
authors.forename: Lai Ling
authors.initials: LL
authors.lastname: Tsang
authors.affiliation: Epithelial Cell Biology Research Center, School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Jiang
authors.affiliation: Department of Gynecology, The Second People's Hospital of Shenzhen, Shenzhen, People's Republic of China.
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Cai
authors.affiliation: Epithelial Cell Biology Research Center, School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong. Electronic address: hsiaocchan@cuhk.edu.hk.
authors.forename: Hsiao Chang
authors.initials: HC
authors.lastname: Chan
authors.affiliation: Department of Gynecology, The Second People's Hospital of Shenzhen, Shenzhen, People's Republic of China. Electronic address: doczhouxiaping@163.com.
authors.forename: Xiaping
authors.initials: X
authors.lastname: Zhou
mesh_terms: D000328:Adult; D000906:Antibodies / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D051926:Basigin / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D016022:Case-Control Studies; D053148:Caspase 3 / Q000378:metabolism; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D004715:Endometriosis / Q000235:genetics / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D004717:Endometrium / Q000187:drug effects / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D004848:Epithelium / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D005260:Female; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D010049:Ovarian Diseases / Q000235:genetics / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D013997:Time Factors; D015854:Up-Regulation
chemical_list: D000906:Antibodies; C497886:BSG protein, human; D051926:Basigin; D011065:Poly(ADP-ribose) Polymerases; C505407:CASP3 protein, human; D053148:Caspase 3; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2
keywords: CD147; Endometriosis; apoptosis; human endometrium epithelial cell; migration