title: [Peculiarity of reassortment of current wild type influenza viruses with master donor viruses for live influenza vaccine].
abstract: The live attenuated influenza vaccine (LAIV) currently licensed in Russia consists of the reassortant viruses with hemagglutinin (HA) and neuraminidase (NA) gene segments from the circulating wild-type viruses and the six internal protein-encoding gene segments from cold-adapted master donor viruses (MDV) A/Leningrad/134/17/57 (H2N2) or B/USSR/60/69. Presently, only classical reassortment technique is approved for the generation of Russian LAIV strains. In this work, we describe the obstacles to the development of LAIV 6:2 vaccine strains depending on the phenotypic properties of the wild-type viruses used for reassortment. It was demonstrated that the highest percentage of 6:2 vaccine reassortants could be achieved when wild-type parental viruses were resistant to non-specific gamma-inhibitors. It was shown that it was impossible to generate 6:2 vaccine reassortants possessing six internal genes of the AILeningrad113417/57 (H2N2) master donor virus and avian HA and NA genes from H5N1-PR8 viruses using classical reassortment technique. It was suggested that strong constellation effects between the gene segments of the parental viruses could affect the virus gene reassortment. A strong interaction between the genome segments encoding neuraminidase of avian origin and PB2 gene of PR8 virus was observed. When the PB2 gene was inherited from cold-adapted master donor virus, the neuraminidase was also found to be of MDV origin.
authors.forename: I V
authors.initials: IV
authors.lastname: Kiseleva
authors.forename: E A
authors.initials: EA
authors.lastname: Bazhenova
authors.forename: N V
authors.initials: NV
authors.lastname: Larionova
authors.forename: E A
authors.initials: EA
authors.lastname: Fedorova
authors.forename: I A
authors.initials: IA
authors.lastname: Dubrovina
authors.forename: I N
authors.initials: IN
authors.lastname: Isakova-Sivak
authors.forename: L G
authors.initials: LG
authors.lastname: Rudenko
mesh_terms: D000220:Adaptation, Biological; D003080:Cold Temperature; D005814:Genes, Viral; D008040:Genetic Linkage; D005838:Genotype; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics* / Q000276:immunology; D006801:Humans; D053121:Influenza A Virus, H2N2 Subtype / Q000235:genetics* / Q000276:immunology; D053124:Influenza A Virus, H5N1 Subtype / Q000235:genetics* / Q000276:immunology; D007252:Influenza Vaccines / Q000235:genetics* / Q000276:immunology; D007251:Influenza, Human / Q000276:immunology / Q000517:prevention & control / Q000821:virology; D009439:Neuraminidase / Q000235:genetics* / Q000276:immunology; D016865:Reassortant Viruses / Q000235:genetics* / Q000276:immunology; D012426:Russia; D014613:Vaccines, Attenuated; D014779:Virus Replication
chemical_list: D019267:Hemagglutinin Glycoproteins, Influenza Virus; D007252:Influenza Vaccines; D014613:Vaccines, Attenuated; D009439:Neuraminidase