title: β-Amyloid and neprilysin computational studies identify critical residues implicated in binding specificity.
abstract: The zinc metalloprotease neprilysin (NEP) promiscuously degrades small bioactive peptides. NEP is among a select group of metalloenzymes that degrade the amyloid beta-peptide (Aβ) in vivo and in situ. Since accumulation of neurotoxic Aβ aggregates in the brain appears to be a causative agent in the pathophysiology of Alzheimer's disease (AD), increased clearance of Aβ resulting from overexpression of NEP exhibits therapeutic potential for AD. However, higher NEP peptidase activity may be harmful without an increased specificity for Aβ over other competing substrates. Crystal structures of NEP-inhibitor complexes and their characterization have highlighted potential amino acid interactions involved in substrate binding and are used as templates to guide our methodology in docking Aβ in NEP. Results from protein-ligand docking calculations predict S2' subsite residues Arg 102 and Arg 110 of NEP participate in specific interactions with Aβ. These interactions provide insight into developing NEP specificity for Aβ.
authors.affiliation: Department of Chemistry and Biochemistry and Center for Computational Sciences, Duquesne University , 600 Forbes Avenue, 331 Mellon Hall, Pittsburgh, Pennsylvania 15282, United States.
authors.forename: Darrick
authors.initials: D
authors.lastname: Pope
authors.forename: Jeffry D
authors.initials: JD
authors.lastname: Madura
authors.forename: Michael
authors.initials: M
authors.lastname: Cascio
mesh_terms: D000595:Amino Acid Sequence; D016229:Amyloid beta-Peptides / Q000737:chemistry* / Q000378:metabolism; D001665:Binding Sites; D018360:Crystallography, X-Ray; D008958:Models, Molecular; D008969:Molecular Sequence Data; D015260:Neprilysin / Q000737:chemistry* / Q000378:metabolism
chemical_list: D016229:Amyloid beta-Peptides; D015260:Neprilysin