title: Induction of HT-29 cells apoptosis by lactobacilli isolated from fermented products.
abstract: A total of 138 lactobacillus strains were obtained from the traditional fermented foods of minority nationalities or infant faeces, respectively. The lactobacillus strains were screened for anticancer effects and probiotic potential. The results showed that 10 strains exerted anti-proliferative activity and higher adhering capability on HT-29 cells. They were then screened for resistance to biological barriers (acid and bile salts), and the four most promising strains were selected. Further analysis revealed that the 4 strains (cell walls and cytoplasm extracts) displayed the high anti-proliferative activity and the large extent of DNA strand breakage in individual cells. Through the selected procedure, cell walls that were extracted from X12, M5 and K14 strains induced apoptosis in HT-29 cells. Further investigation confirmed that apoptosis-inducing ability of cell wall extracts was attributed to the breakdown of mitochondrial membrane potential, which is a known initiation of apoptotic mitochondrial pathway. Cell walls from X12, M5 and K14 strains, were determined to be less harmful to noncancerous Vero cells than to human colon cancer HT-29 cells. These findings suggested that X12, M5 and K14 strains opposing the ability to induce HT-29 cells apoptosis, and cell wall extracts were involved in this apoptosis induction.
authors.affiliation: School of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, China; Harbin University, Harbin 150080, Heilongjiang, China. Electronic address: wangshumei24@126.com.
authors.forename: Shu-Mei
authors.initials: SM
authors.lastname: Wang
authors.affiliation: School of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, China. Electronic address: zhanglw@hit.edu.cn.
authors.forename: Lan-Wei
authors.initials: LW
authors.lastname: Zhang
authors.affiliation: School of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, China; College of Food Science & Engineering, Qingdao Agricultural University, Qingdao 266109, China. Electronic address: 15590011@qq.com.
authors.forename: Rong-Bo
authors.initials: RB
authors.lastname: Fan
authors.affiliation: School of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, China. Electronic address: 282964299@qq.com.
authors.forename: Xue
authors.initials: X
authors.lastname: Han
authors.affiliation: School of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, China. Electronic address: 46511375@qq.com.
authors.forename: Hua-Xi
authors.initials: HX
authors.lastname: Yi
authors.affiliation: School of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, China. Electronic address: 38521738@qq.com.
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Zhang
authors.affiliation: School of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, China. Electronic address: 873790844@qq.com.
authors.forename: Chao-Hui
authors.initials: CH
authors.lastname: Xue
authors.affiliation: School of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, China. Electronic address: 76717064@qq.com.
authors.forename: Hong-Bo
authors.initials: HB
authors.lastname: Li
authors.affiliation: School of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, China. Electronic address: 171849807@qq.com.
authors.forename: Yan-He
authors.initials: YH
authors.lastname: Zhang
authors.affiliation: School of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, China; University of Namibia, Department of Food Science and Technology, Windhoek, Namibia. Electronic address: 112161590@qq.com.
authors.forename: Nditange
authors.initials: N
authors.lastname: Shigwedha
mesh_terms: D000898:Antibiosis; D017209:Apoptosis; D001422:Bacterial Adhesion; D049109:Cell Proliferation; D002473:Cell Wall / Q000378:metabolism*; D004847:Epithelial Cells / Q000382:microbiology* / Q000502:physiology*; D005516:Food Microbiology; D019073:HT29 Cells; D006801:Humans; D007778:Lactobacillus / Q000302:isolation & purification / Q000502:physiology*
keywords: Anti-proliferative activity; Cell wall; Cytoplasm; HT-29 cells apoptosis-inducing; Lactobacillus