title: [Accumulation of the bvg- Bordetella pertussis a virulent mutants in the process of experimental whooping cough in mice].
abstract: The duration of the persistence and dynamics of accumulation of insertion bvg- Bordetella pertussis mutants were studied in lungs of laboratory mice after intranasal and intravenous challenge by virulent bacteria of the causative agent of whooping cough. The capability of the virulent B. pertussis bacteria to long-term persistence in the body of mice was tested. Using the real-time PCR approximately hundred genome equivalents of the B. pertussis DNA were detected in lungs of mice in two months after infection regardless of the way of challenge. Using the bacterial test bacteria were identified during only four weeks after challenge. Bvg- B. pertussis avirulent mutants were accumulated for the infection time. The percentage of the avirulent bacteria in the B. pertussis population reached 50% in 7-9 weeks after challenge. The obtained results show that the laboratory mice can be used for study of the B. pertussis insertion mutant formation dynamics in vivo and confirm the hypothesis about insertional bvg- B. pertussis virulent mutants accumulation during development of pertussis infection in human.
authors.forename: A Iu
authors.initials: AIu
authors.lastname: Medkova
authors.forename: L N
authors.initials: LN
authors.lastname: Siniashina
authors.forename: Iu P
authors.initials: IuP
authors.lastname: Rumiantseva
authors.forename: O L
authors.initials: OL
authors.lastname: Voronina
authors.forename: M S
authors.initials: MS
authors.lastname: Kunda
authors.forename: G I
authors.initials: GI
authors.lastname: Karataev
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D001886:Bordetella pertussis / Q000235:genetics / Q000472:pathogenicity*; D004195:Disease Models, Animal; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*; D014774:Virulence / Q000235:genetics; D014917:Whooping Cough / Q000235:genetics* / Q000382:microbiology / Q000473:pathology
chemical_list: D001426:Bacterial Proteins; C065601:BvgA protein, Bacteria; D014157:Transcription Factors; C080662:bvgS protein, Bordetella pertussis