title: Rapid measurement of mitotic spindle orientation in cultured mammalian cells.
abstract: Factors that influence the orientation of the mitotic spindle are important for the maintenance of stem cell populations and in cancer development. However, screening for these factors requires rapid quantification of alterations of the angle of the mitotic spindle in cultured cell lines. Here we describe a method to image mitotic cells and rapidly score the angle of the mitotic spindle using a simple MATLAB application to analyze a stack of Z-images.
authors.affiliation: Department of Physiology and Biophysics, University of Washington, Seattle, WA, USA.
authors.forename: Justin
authors.initials: J
authors.lastname: Decarreau
authors.forename: Jonathan
authors.initials: J
authors.lastname: Driver
authors.forename: Charles
authors.initials: C
authors.lastname: Asbury
authors.forename: Linda
authors.initials: L
authors.lastname: Wordeman
mesh_terms: D002460:Cell Line; D002478:Cells, Cultured; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008856:Microscopy, Fluorescence; D008938:Mitosis / Q000502:physiology*; D057054:Molecular Imaging / Q000379:methods*; D012984:Software; D008941:Spindle Apparatus / Q000378:metabolism*