title: Biorefinery development through utilization of biodiesel industry by-products as sole fermentation feedstock for 1,3-propanediol production.
abstract: Rapeseed meal (RSM) hydrolysate was evaluated as substitute for commercial nutrient supplements in 1,3-propanediol (PDO) fermentation using the strain Clostridium butyricum VPI 1718. RSM was enzymatically converted into a generic fermentation feedstock, enriched in amino acids, peptides and various micro-nutrients, using crude enzyme consortia produced via solid state fermentation by a fungal strain of Aspergillus oryzae. Initial free amino nitrogen concentration influenced PDO production in batch cultures. RSM hydrolysates were compared with commercial nutrient supplements regarding PDO production in fed-batch cultures carried out in a bench-scale bioreactor. The utilization of RSM hydrolysates in repeated batch cultivation resulted in a PDO concentration of 65.5 g/L with an overall productivity of 1.15 g/L/h that was almost 2 times higher than the productivity achieved when yeast extract was used as nutrient supplement.
authors.affiliation: Department of Food Science and Human Nutrition, Agricultural University of Athens, 75 Iera Odos, 11855 Athens, Greece.
authors.forename: Afroditi
authors.initials: A
authors.lastname: Chatzifragkou
authors.affiliation: Department of Food Science and Human Nutrition, Agricultural University of Athens, 75 Iera Odos, 11855 Athens, Greece.
authors.forename: Seraphim
authors.initials: S
authors.lastname: Papanikolaou
authors.affiliation: Department of Food Science and Human Nutrition, Agricultural University of Athens, 75 Iera Odos, 11855 Athens, Greece.
authors.forename: Nikolaos
authors.initials: N
authors.lastname: Kopsahelis
authors.affiliation: Department of Food Science and Human Nutrition, Agricultural University of Athens, 75 Iera Odos, 11855 Athens, Greece.
authors.forename: Vasiliki
authors.initials: V
authors.lastname: Kachrimanidou
authors.affiliation: Department of Physical Chemistry and Applied Thermodynamics, Escuela Politecnica Superior, University of Cordoba, Campus de Excelencia Agroalimentario, ceiA3, 23071 Cordoba, Spain.
authors.forename: Maria Pilar
authors.initials: MP
authors.lastname: Dorado
authors.affiliation: Department of Food Science and Human Nutrition, Agricultural University of Athens, 75 Iera Odos, 11855 Athens, Greece. Electronic address: akoutinas@aua.gr.
authors.forename: Apostolis A
authors.initials: AA
authors.lastname: Koutinas
mesh_terms: D061249:Batch Cell Culture Techniques; D056804:Biofuels / Q000382:microbiology; D019149:Bioreactors / Q000382:microbiology; D001709:Biotechnology / Q000379:methods*; D029741:Brassica rapa / Q000378:metabolism; D050260:Carbohydrate Metabolism; D046972:Clostridium butyricum / Q000254:growth & development / Q000378:metabolism; D005285:Fermentation; D005990:Glycerol / Q000378:metabolism; D006868:Hydrolysis; D011409:Propylene Glycols / Q000378:metabolism*; D059748:Proteolysis; D013696:Temperature
chemical_list: D056804:Biofuels; D011409:Propylene Glycols; C041787:1,3-propanediol; D005990:Glycerol
keywords: 1,3-Propanediol; Biorefineries; Clostridium butyricum; Crude glycerol; Rapeseed meal hydrolysate