title: Immortalized porcine intestinal epithelial cell cultures susceptible to porcine rotavirus infection.
abstract: In vitro studies related to various viral pathogenesis in swine have been hampered by the lack of relevant porcine cell lines. The susceptibility to porcine rotavirus infection was evaluated by using a newly established porcine intestinal epithelial cell line. Immunohistochemical staining for cytokeratin confirmed that the cultured cells were epithelial cells. Measurement of cell viability and detection of infected cells confirmed that these epithelial cells were susceptible to porcine rotavirus infection. This study describes the cytopathic changes in cultured porcine intestinal epithelial cells during virus invasion. Following infection with porcine rotavirus, the cell cultures contained viral protein at 16 h post-infection as detected by direct immunofluorescence. The epithelial cell cultures provided competent target cells for studying host cell responses to porcine rotavirus and a homologous system for investigating the response of intestinal epithelial cells during viral infection.
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shaanxi, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shaanxi, China; Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Northwest A&F University, Yangling 712100, Shaanxi, China.
authors.forename: Guangdong
authors.initials: G
authors.lastname: Hu
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shaanxi, China.
authors.forename: Wanjun
authors.initials: W
authors.lastname: Gao
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shaanxi, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Xu
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shaanxi, China.
authors.forename: Pengbo
authors.initials: P
authors.lastname: Ning
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shaanxi, China. Electronic address: yanmingzhang1956@163.com.
authors.forename: Yanming
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002460:Cell Line; D002470:Cell Survival; D004847:Epithelial Cells / Q000737:chemistry / Q000821:virology*; D007150:Immunohistochemistry; D007633:Keratins / Q000032:analysis; D012401:Rotavirus / Q000254:growth & development*; D013552:Swine; D014776:Virus Cultivation / Q000379:methods
chemical_list: D007633:Keratins
keywords: Cultures; Porcine intestinal epithelial cell; Porcine rotavirus infection; Susceptivity