title: Guinea pig cytomegalovirus GP129/131/133, homologues of human cytomegalovirus UL128/130/131A, are necessary for infection of monocytes and macrophages.
abstract: The GP129, GP131 and GP133 genes of guinea pig cytomegalovirus (GPCMV) are homologues of human cytomegalovirus UL128, UL130 and UL131A, respectively, which are essential for infection of endothelial and epithelial cells, and for viral transmission to leukocytes. Our previous study demonstrated that a GPCMV strain lacking the 1.6 kb locus that contains the GP129, GP131 and GP133 genes had a growth defect in animals. Here, we demonstrated that the WT strain, but not the 1.6 kb-deleted strain, formed capsids in macrophages prepared from the peritoneal fluid. To understand the mechanism, we prepared GPCMV strains defective in each of GP129, GP131 and GP133, and found that they were all essential for the infection of peritoneal, splenic and PBMC-derived macrophages/monocytes, and for expression of immediate-early antigens in the macrophages/monocytes, although they were dispensable for infection of fibroblasts. Monocyte/macrophage tropism could be one of the important determinants for viral dissemination in vivo.
authors.affiliation: Department of Virology I, National Institute of Infectious Diseases, Tokyo, Japan.
authors.forename: Souichi
authors.initials: S
authors.lastname: Yamada
authors.affiliation: Department of Virology I, National Institute of Infectious Diseases, Tokyo, Japan.
authors.forename: Saki
authors.initials: S
authors.lastname: Fukuchi
authors.affiliation: Department of Virology I, National Institute of Infectious Diseases, Tokyo, Japan.
authors.forename: Kaede
authors.initials: K
authors.lastname: Hashimoto
authors.affiliation: Department of Virology I, National Institute of Infectious Diseases, Tokyo, Japan.
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Fukui
authors.affiliation: Department of Virology I, National Institute of Infectious Diseases, Tokyo, Japan.
authors.forename: Mihoko
authors.initials: M
authors.lastname: Tsuda
authors.affiliation: Department of Pathology, National Institute of Infectious Diseases, Tokyo, Japan.
authors.forename: Michiyo
authors.initials: M
authors.lastname: Kataoka
authors.affiliation: Department of Pathology, National Institute of Infectious Diseases, Tokyo, Japan.
authors.forename: Harutaka
authors.initials: H
authors.lastname: Katano
authors.affiliation: Department of Microbiology and Immunology, Gifu Pharmaceutical University, Gifu, Japan.|Department of Virology I, National Institute of Infectious Diseases, Tokyo, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Inoue
mesh_terms: D000818:Animals; D003587:Cytomegalovirus / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology; D017353:Gene Deletion; D017781:Genes, Immediate-Early; D005814:Genes, Viral; D006168:Guinea Pigs; D006801:Humans; D017737:Macrophages, Peritoneal / Q000821:virology*; D008562:Membrane Glycoproteins / Q000235:genetics / Q000502:physiology; D009000:Monocytes / Q000821:virology*; D019211:Roseolovirus / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology; D013045:Species Specificity; D014759:Viral Envelope Proteins / Q000235:genetics / Q000502:physiology; D014764:Viral Proteins / Q000235:genetics / Q000502:physiology*; D014774:Virulence / Q000235:genetics / Q000502:physiology
chemical_list: D008562:Membrane Glycoproteins; C532797:UL128 protein, human cytomegalovirus; C502890:UL130 protein, human cytomegalovirus; D014759:Viral Envelope Proteins; D014764:Viral Proteins