title: Nocardioides soli sp. nov., a carbendazim-degrading bacterium isolated from soil under the long-term application of carbendazim.
abstract: The taxonomic status of a carbendazim-degrading strain, mbc-2(T), isolated from soil under the long-term application of carbendazim in China was determined by means of a polyphasic study. The cells were Gram-stain-positive, motile and rod-shaped. Strain mbc-2(T) grew optimally at pH 7.0, 30-35 °C and in the presence of 1% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain mbc-2(T) fell within the genus Nocardioides, forming a coherent cluster with the type strain of Nocardioides hankookensis, with which it exhibited 16S rRNA gene sequence similarity values of 97.9%. The chemotaxonomic properties of strain mbc-2(T) were consistent with those of the genus Nocardioides: the cell-wall peptidoglycan type was based on ll-2,6-diaminopimelic acid, the predominant menaquinone was MK-8 (H4) and the major fatty acid was iso-C(16 : 0). The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, unknown phospholipids and an unknown aminolipid. The DNA G+C content was 72 mol%. Strain mbc-2(T) exhibited DNA-DNA relatedness values of 12.5±1.5%, 23.7±2.7% and 26.3±3.2% with respect to Nocardioides hankookensis DS-30(T), Nocardioides aquiterrae GW-9(T) and Nocardioides pyridinolyticus OS4(T). On the basis of the data obtained, strain mbc-2(T) represents a novel species of the genus Nocardioides, for which the name Nocardioides soli sp. nov. is proposed. The type strain is mbc-2(T) ( = KACC 17152(T) = CCTCC AB 2012934(T)).
authors.affiliation: Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Li-Na
authors.initials: LN
authors.lastname: Sun
authors.affiliation: College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Fen-Fen
authors.initials: FF
authors.lastname: Gong
authors.affiliation: Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Wang
authors.affiliation: Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Gang
authors.initials: G
authors.lastname: Hu
authors.affiliation: Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Shun-Peng
authors.initials: SP
authors.lastname: Li
authors.affiliation: Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Hong
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001562:Benzimidazoles / Q000378:metabolism*; D002219:Carbamates / Q000378:metabolism*; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D005659:Fungicides, Industrial / Q000378:metabolism; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D001562:Benzimidazoles; D002219:Carbamates; D004269:DNA, Bacterial; D005227:Fatty Acids; D005659:Fungicides, Industrial; D010457:Peptidoglycan; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; D024482:Vitamin K 2; C024223:vitamin MK 8; D003960:Diaminopimelic Acid; C006698:carbendazim