title: Synergistic degradation of deca-BDE by an enrichment culture and zero-valent iron.
abstract: Debromination of decabromodiphenyl ether (deca-BDE) by microbe and by zero-valent iron (ZVI) has been reported previously. However, no study has indicated the presence of microorganisms and their effect on ZVI-mediated reduction of deca-BDE. Synergistic degradation of deca-BDE by an enrichment culture and ZVI was studied. It was found that synergistic effects enhanced the debromination of deca-BDE as well as promoting the reduction of lower brominated products. ZVI stimulated microbial debromination by serving as an electron donor. Correlation analysis also confirmed that ZVI was capable of enhancing microbial population in the debromination of deca-BDE. Conversely, the enrichment culture produced acid which maintained pH stability and stimulated the oxidation of ZVI. The enrichment culture supplied its energy requirements by the oxidation of ZVI and concomitant reduction of deca-BDE, but incapable of growth and reduction of BDE-209 without ZVI and vice versa. Compared to the initial culture, the microbial community of the enrichment culture became dominated by several bacterial genera based on the results of 16S rRNA-gene pyrosequencing.
authors.affiliation: Guangdong Institute of Microbiology, Guangzhou, 510070, Guangdong Province, China.
authors.forename: Xingjuan
authors.initials: X
authors.lastname: Chen
authors.forename: Guilan
authors.initials: G
authors.lastname: Chen
authors.forename: Mengde
authors.initials: M
authors.lastname: Qiu
authors.forename: Guoping
authors.initials: G
authors.lastname: Sun
authors.forename: Jun
authors.initials: J
authors.lastname: Guo
authors.forename: Meiying
authors.initials: M
authors.lastname: Xu
mesh_terms: D001419:Bacteria / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D001673:Biodegradation, Environmental; D016000:Cluster Analysis; D017931:DNA Primers / Q000235:genetics; D019015:Geologic Sediments / Q000737:chemistry*; D055768:Halogenated Diphenyl Ethers / Q000737:chemistry* / Q000378:metabolism*; D054879:Halogenation; D007501:Iron / Q000737:chemistry*; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000378:metabolism; D045483:Rivers / Q000737:chemistry*; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D055768:Halogenated Diphenyl Ethers; D012336:RNA, Ribosomal, 16S; D007501:Iron; C010902:decabromobiphenyl ether