title: Chemical functionalization of hyaluronic acid for drug delivery applications.
abstract: Functionalized hyaluronic acid (HA) derivatives were obtained by ring opening mechanism of maleic anhydride (MA). FTIR and H(1) NMR spectroscopy were used to confirm the chemical linkage of MA on the hyaluronic acid chains. Thermal analysis (TG-DTG and DSC) and GPC data for the new products revealed the formation of new functional groups, without significant changes in molecular weight and thermal stability. New gels based on hyaluronic acid modified derivatives were obtained by acrylic acid copolymerization in the presence of a redox initiation system. The resulted circular and interconnected pores of the gels were visualized by SEM. The release profiles of an ophthalmic model drug, pilocarpine from tested gels were studied in simulated media. Evaluation of the cytotoxicity and cell proliferation properties indicates the potential of the new systems to be used in contact with biological media in drug delivery applications.
authors.affiliation: "Gheorghe Asachi" Technical University of Iasi, Faculty of Chemical Engineering and Environmental Protection, 73 Prof. dr. docent Dimitrie Mangeron Street, 700050 Iasi, Romania.
authors.forename: Ana-Maria
authors.initials: AM
authors.lastname: Vasi
authors.affiliation: "Gheorghe Asachi" Technical University of Iasi, Faculty of Chemical Engineering and Environmental Protection, 73 Prof. dr. docent Dimitrie Mangeron Street, 700050 Iasi, Romania. Electronic address: mipopa@ch.tuiasi.ro.
authors.forename: Marcel Ionel
authors.initials: MI
authors.lastname: Popa
authors.affiliation: "Grigore T. Popa" University of Medicine Pharmacy, Faculty of Medical Bioengineering, 9-13 Kogalniceanu Street, 700454 Iasi, Romania.
authors.forename: Maria
authors.initials: M
authors.lastname: Butnaru
authors.affiliation: "Gheorghe Asachi" Technical University of Iasi, Faculty of Chemical Engineering and Environmental Protection, 73 Prof. dr. docent Dimitrie Mangeron Street, 700050 Iasi, Romania; SCIENT - Research Center for Instrumental Analysis, S.C. CROMATEC PLUS, 18 Sos. Cotroceni, 060114 Bucharest, Romania.
authors.forename: Gianina
authors.initials: G
authors.lastname: Dodi
authors.affiliation: "Grigore T. Popa" University of Medicine Pharmacy, Faculty of Medical Bioengineering, 9-13 Kogalniceanu Street, 700454 Iasi, Romania.
authors.forename: Liliana
authors.initials: L
authors.lastname: Verestiuc
mesh_terms: D002138:Calibration; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D002850:Chromatography, Gel; D016503:Drug Delivery Systems / Q000379:methods*; D005782:Gels / Q000737:chemistry; D005936:Glucans / Q000737:chemistry; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry*; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D008855:Microscopy, Electron, Scanning; D010862:Pilocarpine / Q000494:pharmacology; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D013818:Thermogravimetry
chemical_list: D005782:Gels; D005936:Glucans; D010862:Pilocarpine; C009109:pullulan; D006820:Hyaluronic Acid
keywords: Acrylic acid; Functionalized hyaluronic acid; Gels; Pilocarpine; Ring opening mechanism