title: An orthogonal fusion tag for efficient protein purification.
abstract: Protein fusion tags are important tools in research when robust methods for protein purification and detection are required. In this chapter we present an efficient method for stringent protein purification. A small domain, denoted ABDz1, with affinity for both human serum albumin and Protein A has been developed. The purification tag is based on an albumin-binding domain from Streptococcal Protein G that was engineered to bind Protein A. The ABDz1-tag can be fused to any protein of choice and the purification can be performed using standard laboratory equipment. In this chapter a method for purification of ABDz1-tagged proteins using two successive affinity purification steps is described.
authors.affiliation: Division of Proteomics, School of Biotechnology, KTH/AlbaNova University Center, Stockholm, Sweden.
authors.forename: Johan
authors.initials: J
authors.lastname: Nilvebrant
authors.forename: Mikael
authors.initials: M
authors.lastname: Astrand
authors.forename: Sophia
authors.initials: S
authors.lastname: Hober
mesh_terms: D002846:Chromatography, Affinity / Q000379:methods; D011485:Protein Binding; D011506:Proteins / Q000302:isolation & purification*
chemical_list: D011506:Proteins