title: Analysis of autofluorescence in polymorphonuclear neutrophils: a new tool for early infection diagnosis.
abstract: Diagnosing bacterial infection (BI) remains a challenge for the attending physician. An ex vivo infection model based on human fixed polymorphonuclear neutrophils (PMNs) gives an autofluorescence signal that differs significantly between stimulated and unstimulated cells. We took advantage of this property for use in an in vivo pneumonia mouse model and in patients hospitalized with bacterial pneumonia. A 2-fold decrease was observed in autofluorescence intensity for cytospined PMNs from broncho-alveolar lavage (BAL) in the pneumonia mouse model and a 2.7-fold decrease was observed in patients with pneumonia when compared with control mice or patients without pneumonia, respectively. This optical method provided an autofluorescence mean intensity cut-off, allowing for easy diagnosis of BI. Originally set up on a confocal microscope, the assay was also effective using a standard epifluorescence microscope. Assessing the autofluorescence of PMNs provides a fast, simple, cheap and reliable method optimizing the efficiency and the time needed for early diagnosis of severe infections. Rationalized therapeutic decisions supported by the results from this method can improve the outcome of patients suspected of having an infection.
authors.affiliation: Multidisciplinary Intensive Care Unit, Department of Anesthesiology and Critical Care, La Pitié-Salpêtrière Hospital, Assistance Publique-Hôpitaux de Paris, Paris, France; UPMC Université Paris, Paris, France.
authors.forename: Antoine
authors.initials: A
authors.lastname: Monsel
authors.affiliation: CPBM/CLUPS, université Paris-Sud, Orsay Cedex, France.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Lécart
authors.affiliation: Laboratoire UPRES EA 3826, Thérapeutiques cliniques et expérimentales des infections, Faculté de Médecine, Faculté de Nantes, Nantes, France; CHU Nantes, Pôle Anesthésie Réanimation, Service d'Anesthésie Réanimation Chirurgicale, Hôtel Dieu, Nantes, France.
authors.forename: Antoine
authors.initials: A
authors.lastname: Roquilly
authors.affiliation: Laboratoire UPRES EA 3826, Thérapeutiques cliniques et expérimentales des infections, Faculté de Médecine, Faculté de Nantes, Nantes, France.
authors.forename: Alexis
authors.initials: A
authors.lastname: Broquet
authors.affiliation: Laboratoire UPRES EA 3826, Thérapeutiques cliniques et expérimentales des infections, Faculté de Médecine, Faculté de Nantes, Nantes, France.
authors.forename: Cédric
authors.initials: C
authors.lastname: Jacqueline
authors.affiliation: Vascular Medicine Unit, European Hospital Georges-Pompidou, Assistance Publique-Hôpitaux de Paris, Paris Descartes University, PRES Paris Sorbonne Cité, Paris, France.
authors.forename: Tristan
authors.initials: T
authors.lastname: Mirault
authors.affiliation: ISMO, CNRS- Université Paris-Sud, Orsay, France.
authors.forename: Thibaut
authors.initials: T
authors.lastname: Troude
authors.affiliation: CPBM/CLUPS, université Paris-Sud, Orsay Cedex, France; ISMO, CNRS- Université Paris-Sud, Orsay, France.
authors.forename: Marie-Pierre
authors.initials: MP
authors.lastname: Fontaine-Aupart
authors.affiliation: Laboratoire UPRES EA 3826, Thérapeutiques cliniques et expérimentales des infections, Faculté de Médecine, Faculté de Nantes, Nantes, France; CHU Nantes, Pôle Anesthésie Réanimation, Service d'Anesthésie Réanimation Chirurgicale, Hôtel Dieu, Nantes, France.
authors.forename: Karim
authors.initials: K
authors.lastname: Asehnoune
mesh_terms: D055371:Acute Lung Injury / Q000139:chemically induced / Q000175:diagnosis; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology; D006801:Humans; D008070:Lipopolysaccharides; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008856:Microscopy, Fluorescence / Q000379:methods*; D023421:Models, Animal; D009504:Neutrophils / Q000382:microbiology*; D018410:Pneumonia, Bacterial / Q000175:diagnosis* / Q000382:microbiology; D053717:Pneumonia, Ventilator-Associated / Q000175:diagnosis
chemical_list: D008070:Lipopolysaccharides