title: Canine T-zone lymphoma: unique immunophenotypic features, outcome, and population characteristics.
abstract: BACKGROUND
  Canine T-cell lymphoma (TCL) is clinically and histologically heterogeneous with some forms, such as T-zone lymphoma (TZL), having an indolent course. Immunophenotyping is an important tool in the classification of TCL in people, and can be equally useful in dogs.
  
  
  OBJECTIVE
  We hypothesized that loss of expression of the CD45 antigen is a specific diagnostic feature of TZL.
  
  
  METHODS
  Twenty dogs with concurrent histology and immunophenotyping by flow cytometry were studied in depth. An additional 494 dogs diagnosed by immunophenotyping were used to characterize the population of dogs with this disease.
  
  
  METHODS
  Lymph node biopsies from 35 dogs with TCL were classified by 2 pathologists using WHO criteria. Twenty lymph nodes were from dogs with CD45- TCL and 15 were from CD45+ TCL. The pathologists were blinded to the flow cytometry findings. Outcome information was sought for the 20 dogs with CD45- lymphoma, and population characteristics of the additional 494 dogs were described.
  
  
  RESULTS
  All 20 CD45- cases were classified as TZL. The 15 CD45+ cases were classified as aggressive TCL and are described in an accompanying paper. TZL cases had a median survival of 637 days. Examination of 494 additional dogs diagnosed with TZL by immunophenotyping demonstrated that 40% of cases are in Golden Retrievers, are diagnosed at a median age of 10 years, and the majority have lymphadenopathy and lymphocytosis.
  
  
  CONCLUSIONS
  TZL has unique immunophenotypic features that can be used for diagnosis.
authors.affiliation: Veterinary Clinical Sciences, University of Minnesota, St. Paul, MN.
authors.forename: D M
authors.initials: DM
authors.lastname: Seelig
authors.forename: P
authors.initials: P
authors.lastname: Avery
authors.forename: T
authors.initials: T
authors.lastname: Webb
authors.forename: J
authors.initials: J
authors.lastname: Yoshimoto
authors.forename: J
authors.initials: J
authors.lastname: Bromberek
authors.forename: E J
authors.initials: EJ
authors.lastname: Ehrhart
authors.forename: A C
authors.initials: AC
authors.lastname: Avery
mesh_terms: D000818:Animals; D004283:Dog Diseases / Q000175:diagnosis / Q000276:immunology* / Q000473:pathology; D004285:Dogs; D005260:Female; D005434:Flow Cytometry / Q000662:veterinary; D016130:Immunophenotyping; D017493:Leukocyte Common Antigens / Q000276:immunology; D008198:Lymph Nodes / Q000473:pathology; D016399:Lymphoma, T-Cell / Q000175:diagnosis / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D008297:Male
chemical_list: D017493:Leukocyte Common Antigens
keywords: Flow cytometry; Golden retriever; Leukemia