title: Construction and functional identification of a hepatitis B virus S protein small hairpin RNA recombinant adenovirus.
abstract: Hepatitis B virus S protein (HBs) plays an important role in hepatocellular carcinoma progression. However, to date, no direct and effective methods exist to research the function of HBs. Here, we combined the technology of RNA interference with recombinant adenovirus, constructed a recombinant adenovirus-expressing small hairpin RNA of HBs, and infected HepG2.2.15 cells. Then, reverse transcription-polymerase chain reaction (RT-PCR), quantitative real-time PCR, enzyme-linked immunosorbent assay, and Western blot analysis were performed to verify the interference effects. As a result, a recombinant adenovirus was successfully constructed and effectively packaged in AD293 cells, and it significantly inhibited HBs mRNA and protein expression in vitro. Our study may provide a novel tool to study HBs function.
authors.affiliation: Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Second Affiliated Hospital, Chongqing Medical University, Chongqing, China.
authors.forename: S
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Forensic Medicine, Chongqing Medical University, Chongqing, China.
authors.forename: Y Z
authors.initials: YZ
authors.lastname: Liu
authors.affiliation: Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Second Affiliated Hospital, Chongqing Medical University, Chongqing, China.
authors.forename: D D
authors.initials: DD
authors.lastname: Qin
authors.affiliation: Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Second Affiliated Hospital, Chongqing Medical University, Chongqing, China.
authors.forename: C C
authors.initials: CC
authors.lastname: Zou
authors.affiliation: Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Second Affiliated Hospital, Chongqing Medical University, Chongqing, China.
authors.forename: Y R
authors.initials: YR
authors.lastname: Sheng
authors.affiliation: Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Second Affiliated Hospital, Chongqing Medical University, Chongqing, China.
authors.forename: A L
authors.initials: AL
authors.lastname: Huang
authors.affiliation: Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Second Affiliated Hospital, Chongqing Medical University, Chongqing, China tanghua86162003@aliyun.com.
authors.forename: H
authors.initials: H
authors.lastname: Tang
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D015967:Gene Expression Regulation, Viral; D056945:Hep G2 Cells; D006515:Hepatitis B virus / Q000235:genetics* / Q000472:pathogenicity; D006801:Humans; D017293:Protein S / Q000235:genetics* / Q000302:isolation & purification; D012333:RNA, Messenger / Q000096:biosynthesis; D034741:RNA, Small Interfering / Q000235:genetics*
chemical_list: D017293:Protein S; D012333:RNA, Messenger; D034741:RNA, Small Interfering