title: Effect of probiotics on antioxidant and antimutagenic activities of crude peptide extract from yogurt.
abstract: Search for bioactive peptides is intensifying because of the risks associated with the use of synthetic therapeutics, thus peptide liberation by lactic acid bacteria and probiotics has received a great focus. However, proteolytic capacity of these bacteria is strain specific. The study was conducted to establish proteolytic activity of Lactobacillus acidophilus (ATCC® 4356™), Lactobacillus casei (ATCC® 393™) and Lactobacillus paracasei subsp. paracasei (ATCC® BAA52™) in yogurt. Crude peptides were separated by high-speed centrifugation and tested for antioxidant and antimutagenic activities. The degree of proteolysis highly correlated with these bioactivities, and its value (11.91%) for samples containing all the cultures was double that of the control. Liberated peptides showed high radical scavenging activities with 1,1-diphenyl-2-picrylhydrazyl and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid), IC50 1.51 and 1.63mg/ml, respectively and strong antimutagenicity (26.35%). These probiotics enhanced the generation of bioactive peptides and could possibly be commercially applied in new products, or production of novel anticancer peptides.
authors.affiliation: College of Health and Biomedicine, Victoria University, Werribee Campus, PO Box 14428, Melbourne, Victoria 8001, Australia.
authors.forename: B N P
authors.initials: BN
authors.lastname: Sah
authors.affiliation: College of Health and Biomedicine, Victoria University, Werribee Campus, PO Box 14428, Melbourne, Victoria 8001, Australia.
authors.forename: T
authors.initials: T
authors.lastname: Vasiljevic
authors.affiliation: College of Engineering and Science, Victoria University, Werribee Campus, PO Box 14428, Melbourne, Victoria 8001, Australia.
authors.forename: S
authors.initials: S
authors.lastname: McKechnie
authors.affiliation: College of Health and Biomedicine, Victoria University, Werribee Campus, PO Box 14428, Melbourne, Victoria 8001, Australia. Electronic address: Osaana.Donkor@vu.edu.au.
authors.forename: O N
authors.initials: ON
authors.lastname: Donkor
mesh_terms: D016587:Antimutagenic Agents / Q000737:chemistry*; D000975:Antioxidants / Q000737:chemistry*; D007779:Lactobacillus acidophilus / Q000737:chemistry*; D007780:Lacticaseibacillus casei / Q000737:chemistry*; D019936:Probiotics / Q000737:chemistry*; D015014:Yogurt / Q000382:microbiology*
chemical_list: D016587:Antimutagenic Agents; D000975:Antioxidants
keywords: Antimutagenic activity; Antioxidant activity; Degree of hydrolysis; Peptides; Probiotics