title: Disk-shaped amperometric enzymatic biosensor for in vivo detection of D-serine.
abstract: At the synapse, D-serine is an endogenous co-agonist for the N-methyl-D-aspartate receptor (NMDAR). It plays an important role in synaptic transmission and plasticity and has also been linked to several pathological diseases such as schizophrenia and Huntington's. The quantification of local changes in D-serine concentration is essential to further understanding these processes. We report herein the development of a disk-shaped amperometric enzymatic biosensor for detection of D-serine based on a 25 μm diameter platinum disk microelectrode with an electrodeposited poly-m-phenylenediamine (PPD) layer and an R. gracilis D-amino acid oxidase (RgDAAO) layer. The disk-shaped D-serine biosensor is 1-5 orders of magnitude smaller than previously reported probes and exhibits a sensitivity of 276 μA cm(-2) mM(-1) with an in vitro detection limit of 0.6 μM. We demonstrate its usefulness for in vivo applications by measuring the release of endogenous D-serine in the brain of Xenopus laevis tadpoles.
authors.affiliation: Department of Chemistry, McGill University , 801 Sherbrooke Street West, Montreal, Quebec, Canada H3A 0B8.
authors.forename: David
authors.initials: D
authors.lastname: Polcari
authors.forename: Annie
authors.initials: A
authors.lastname: Kwan
authors.forename: Marion R
authors.initials: MR
authors.lastname: Van Horn
authors.forename: Laurence
authors.initials: L
authors.lastname: Danis
authors.forename: Loredano
authors.initials: L
authors.lastname: Pollegioni
authors.forename: Edward S
authors.initials: ES
authors.lastname: Ruthazer
authors.forename: Janine
authors.initials: J
authors.lastname: Mauzeroll
mesh_terms: D000818:Animals; D015374:Biosensing Techniques; D003605:D-Amino-Acid Oxidase / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000295:instrumentation*; D057230:Limit of Detection; D008839:Microelectrodes; D012694:Serine / Q000032:analysis*; D014982:Xenopus laevis
chemical_list: D012694:Serine; D003605:D-Amino-Acid Oxidase