title: Deletion mutant library for investigation of functional outputs of cyclic diguanylate metabolism in Pseudomonas aeruginosa PA14.
abstract: We constructed a library of in-frame deletion mutants targeting each gene in Pseudomonas aeruginosa PA14 predicted to participate in cyclic di-GMP (c-di-GMP) metabolism (biosynthesis or degradation) to provide a toolkit to assist investigators studying c-di-GMP-mediated regulation by this microbe. We present phenotypic assessments of each mutant, including biofilm formation, exopolysaccharide (EPS) production, swimming motility, swarming motility, and twitch motility, as a means to initially characterize these mutants and to demonstrate the potential utility of this library.
authors.affiliation: Geisel School of Medicine at Dartmouth, Department of Microbiology and Immunology, Hanover, New Hampshire, USA.
authors.forename: Dae-Gon
authors.initials: DG
authors.lastname: Ha
authors.forename: Megan E
authors.initials: ME
authors.lastname: Richman
authors.forename: George A
authors.initials: GA
authors.lastname: O'Toole
mesh_terms: D018441:Biofilms / Q000254:growth & development; D001711:Biotransformation; D006152:Cyclic GMP / Q000031:analogs & derivatives* / Q000378:metabolism; D017353:Gene Deletion; D015723:Gene Library; D008124:Locomotion; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D011135:Polysaccharides, Bacterial / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000378:metabolism* / Q000502:physiology
chemical_list: D011135:Polysaccharides, Bacterial; C062025:bis(3',5')-cyclic diguanylic acid; D006152:Cyclic GMP