title: Expression, purification and crystallization of a dye-decolourizing peroxidase from Dictyostelium discoideum.
abstract: Dye-decolourizing peroxidases are haem-containing peroxidases with broad substrate specificity. Using H2O2 as an electron acceptor, they efficiently decolourize various dyes that are of industrial and environmental relevance, such as anthraquninone- and azo-based dyes. In this study, the dye-decolourizing peroxidase DdDyP from Dictyostelium discoideum was overexpressed in Escherichia coli strain Rosetta(DE3)pLysS, purified and crystallized using the vapour-diffusion method. A native crystal diffracted to 1.65 Å resolution and belonged to space group P4(1)2(1)2, with unit-cell parameters a = b = 141.03, c = 95.56 Å, α = β = γ = 90°. The asymmetric unit contains two molecules.
authors.affiliation: Institute for Biophysical Chemistry, Medizinische Hochschule Hannover, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany.
authors.forename: Amrita
authors.initials: A
authors.lastname: Rai
authors.affiliation: Institute for Biophysical Chemistry, Medizinische Hochschule Hannover, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany.
authors.forename: Roman
authors.initials: R
authors.lastname: Fedorov
authors.affiliation: Institute for Biophysical Chemistry, Medizinische Hochschule Hannover, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany.
authors.forename: Dietmar J
authors.initials: DJ
authors.lastname: Manstein
mesh_terms: D001483:Base Sequence; D003116:Color; D004396:Coloring Agents / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods*; D017931:DNA Primers; D004023:Dictyostelium / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D010544:Peroxidases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D011487:Protein Conformation
chemical_list: D004396:Coloring Agents; D017931:DNA Primers; D010544:Peroxidases
keywords: Dictoystelium discoideum; dye-decolourizing peroxidases; haem-binding protein