title: Evaluation of the automated urine particle analyzer UF-1000i screening for urinary tract infection in nonpregnant women.
abstract: BACKGROUND
  Urinary tract infection (UTI) is a widespread disease in women. Urine culture is still the "gold standard" diagnostic test for UTI, but most of them are negative. To reduce unnecessary culture, we evaluated the automated urine particle analyzer UF-1000i screening for UTI in nonpregnant women.
  
  
  METHODS
  The urine specimens submitted to our laboratory were submitted for culture and tested by the Sysmex UF-1000i. Bacteria and white blood cell (WBC) counts were compared to standard urine culture results to assess the best cutoff values.
  
  
  RESULTS
  In this study, 272 urine samples were included, of which 98 (36.0%) were culture positive with a bacterial cutoff value of 10 x 10(5) CFU/mL. A combination of bacterial (> 95/microL) and/or WBC count (> 24/microL) provided the best screening for UTI, with a sensitivity of 0.99 and a specificity of 0.82 compared with the urine culture.
  
  
  CONCLUSIONS
  Sysmex UF-1000i could be used as a screening test for UTI in nonpregnant women. According to the distribution and range of the bacterial scattergram, we could primarily identify and differentiate between Gram-negative and Gram-positive bacteria.
authors.forename: Qingkai
authors.initials: Q
authors.lastname: Dai
authors.forename: Yongmei
authors.initials: Y
authors.lastname: Jiang
authors.forename: Hua
authors.initials: H
authors.lastname: Shi
authors.forename: Wei
authors.initials: W
authors.lastname: Zhou
authors.forename: Shengjie
authors.initials: S
authors.lastname: Zhou
authors.forename: Hui
authors.initials: H
authors.lastname: Yang
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001331:Automation; D001419:Bacteria / Q000254:growth & development; D015169:Colony Count, Microbial; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007958:Leukocyte Count; D008403:Mass Screening; D008875:Middle Aged; D011247:Pregnancy; D016482:Urinalysis / Q000295:instrumentation*; D014552:Urinary Tract Infections / Q000175:diagnosis* / Q000382:microbiology / Q000652:urine*; D055815:Young Adult