title: Get your high-quality low-cost genome sequence.
abstract: The study of whole-genome sequences has become essential for almost all branches of biological research. Next-generation sequencing (NGS) has revolutionized the scalability, speed, and resolution of sequencing and brought genomic science within reach of academic laboratories that study non-model organisms. Here, we show that a high-quality draft genome of a eukaryote can be obtained at relatively low cost by exploiting a hybrid combination of sequencing strategies.
authors.affiliation: Laboratory of Phytopathology, Wageningen University, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands.
authors.forename: Luigi
authors.initials: L
authors.lastname: Faino
authors.affiliation: Laboratory of Phytopathology, Wageningen University, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands. Electronic address: bart.thomma@wur.nl.
authors.forename: Bart P H J
authors.initials: BP
authors.lastname: Thomma
mesh_terms: D015723:Gene Library; D018745:Genome, Plant / Q000235:genetics*; D059014:High-Throughput Nucleotide Sequencing / Q000191:economics / Q000379:methods*; D010944:Plants / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000191:economics / Q000379:methods / Q000592:standards; D049490:Systems Biology
keywords: eukaryote; fungal genome; genome structure; hybrid assembly; next-generation sequencing (NGS); repetitive element