title: Biosynthesis of a thiamin antivitamin in Clostridium botulinum.
abstract: Bacimethrin-derived 2'-methoxythiamin pyrophosphate inhibits microbial growth by disrupting metabolic pathways dependent on thiamin-utilizing enzymes. This study describes the discovery of the bacimethrin biosynthetic gene cluster of Clostridium botulinum A ATCC 19397 and in vitro reconstitution of bacimethrin biosynthesis from cytidine 5'-monophosphate.
authors.affiliation: Department of Chemistry, Texas A&M University , College Station, Texas 77842, United States.
authors.forename: Lisa E
authors.initials: LE
authors.lastname: Cooper
authors.forename: Seán E
authors.initials: SE
authors.lastname: O'Leary
authors.forename: Tadhg P
authors.initials: TP
authors.lastname: Begley
mesh_terms: D003014:Clostridium botulinum / Q000235:genetics / Q000378:metabolism*; D005810:Multigene Family; D013831:Thiamine / Q000037:antagonists & inhibitors*
chemical_list: D013831:Thiamine