title: Study on the inhibitory effect of total alkaliods of Sophora alopecuroides on osteosarcoma cell growth.
abstract: BACKGROUND
  Ku Dou Zi is the dried whole plant, roots and seeds of Sophora alopecuroides L. in the genus Sophora of family Leguminosae. The entire plant is bitter in taste, cold in nature, and has the heat clearing, detoxifying, pathogenic wind dispelling dampness, analgesic, and insecticidal effects. Modern pharmacological studies have proved that TASA has pharmacological activities of anti-cancer. The objective of this paper was to investigate the inhibitory effect of total alkaloids of Sophora alopecuroides (TASA), on osteosarcoma cell growth and its mechanism.
  
  
  METHODS
  MTT assay and flow cytometry were used to study the inhibitory effect of TASA on human osteosarcoma cell line OS732.
  
  
  RESULTS
  The results showed that the inhibition rates of different concentrations of TASA (1.5, 3, and 4.5g/kg), against human osteosarcoma cell line OS732, were: 18.4%, 27.4% and 52.8%, respectively.
  
  
  CONCLUSIONS
  TASA has an inhibitory effect on osteosarcoma cell growth.
authors.affiliation: Institute of Orthopedics, The First Affiliated Hospital of the General Hospital of PLA, Beijing 100048, People's Republic of China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Lu
authors.affiliation: Institute of Orthopedics, The First Affiliated Hospital of the General Hospital of PLA, Beijing 100048, People's Republic of China.
authors.forename: Bin
authors.initials: B
authors.lastname: Lin
authors.affiliation: Institute of Orthopedics, The First Affiliated Hospital of the General Hospital of PLA, Beijing 100048, People's Republic of China.
authors.forename: Jia-guang
authors.initials: JG
authors.lastname: Tang
authors.affiliation: Institute of Orthopedics, The First Affiliated Hospital of the General Hospital of PLA, Beijing 100048, People's Republic of China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Cao
authors.affiliation: Institute of Orthopedics, The First Affiliated Hospital of the General Hospital of PLA, Beijing 100048, People's Republic of China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Hu
mesh_terms: D000470:Alkaloids / Q000494:pharmacology / Q000627:therapeutic use*; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology / Q000627:therapeutic use*; D001859:Bone Neoplasms / Q000188:drug therapy*; D049109:Cell Proliferation; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use*; D006801:Humans; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D012516:Osteosarcoma / Q000188:drug therapy*; D008517:Phytotherapy; D029909:Sophora / Q000737:chemistry*
chemical_list: D000470:Alkaloids; D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal
keywords: flow cytometry; human osteosarcoma cell line OS732; total alkaloids of Sophora alopecuroides