title: Partial purification and properties of adenosine triphosphatase (ATPase) from liver fluke Fasciola hepatica.
abstract: OBJECTIVE
  The adenosine triphosphatase (ATP phosphohydrolase, EC 3.6.1.3.;ATPase) is a membrane -bound enzyme which transport protons across the plasma membrane using ATP as an energy source.
  
  
  METHODS
  The adenosine triphosphatase (ATPase ; EC: 3.6.1.3) was extracted from membrane preparations of adult Fasciola hepatica by chloroform treatment and purified by means of ammonium sulphate fractionation, gel filtration on sephadex G-200 and DEAE- Cellulose chromatography.
  
  
  RESULTS
  The molecular weight was calculated to be 305.000 dalton by gel filtration. Kinetic experiments demonstrated a biphasic linear lineweaver - burk relationship (km=0.142 and 1.66 mM) thus revealing the existence of two substrate binding enzyme sites.
  
  
  CONCLUSIONS
  In our study revealed that partial inhibition of Mg²⁺ dependent purified enzyme by oligomycin suggest the absence of mitochondrial ATPase in F. hepatica.
authors.affiliation: Department of Biology, Kirkuk College of Science, Iraq.
authors.forename: Husain
authors.initials: H
authors.lastname: Hassan
authors.forename: Ali
authors.initials: A
authors.lastname: Abeer
mesh_terms: D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000302:isolation & purification*; D000818:Animals; D001652:Bile Ducts / Q000469:parasitology; D020134:Catalytic Domain; D002417:Cattle; D002462:Cell Membrane / Q000737:chemistry / Q000201:enzymology; D005210:Fasciola hepatica / Q000737:chemistry* / Q000201:enzymology / Q000302:isolation & purification; D007700:Kinetics; D008970:Molecular Weight; D009840:Oligomycins / Q000737:chemistry; D013379:Substrate Specificity
chemical_list: D009840:Oligomycins; D000251:Adenosine Triphosphatases