title: Noninvasive peroneal sensory and motor nerve conduction recordings in the rabbit distal hindlimb: feasibility, variability and neuropathy measure.
abstract: The peroneal nerve anatomy of the rabbit distal hindlimb is similar to humans, but reports of distal peroneal nerve conduction studies were not identified with a literature search. Distal sensorimotor recordings may be useful for studying rabbit models of length-dependent peripheral neuropathy. Surface electrodes were adhered to the dorsal rabbit foot overlying the extensor digitorum brevis muscle and the superficial peroneal nerve. The deep and superficial peroneal nerves were stimulated above the ankle and the common peroneal nerve was stimulated at the knee. The nerve conduction studies were repeated twice with a one-week intertest interval to determine measurement variability. Intravenous vincristine was used to produce a peripheral neuropathy. Repeat recordings measured the response to vincristine. A compound muscle action potential and a sensory nerve action potential were evoked in all rabbits. The compound muscle action potential mean amplitude was 0.29 mV (SD ± 0.12) and the fibula head to ankle mean motor conduction velocity was 46.5 m/s (SD ± 2.9). The sensory nerve action potential mean amplitude was 22.8 μV (SD ± 2.8) and the distal sensory conduction velocity was 38.8 m/s (SD ± 2.2). Sensorimotor latencies and velocities were least variable between two test sessions (coefficient of variation  =  2.6-5.9%), sensory potential amplitudes were intermediate (coefficient of variation  =  11.1%) and compound potential amplitudes were the most variable (coefficient of variation  = 19.3%). Vincristine abolished compound muscle action potentials and reduced sensory nerve action potential amplitudes by 42-57% while having little effect on velocity. Rabbit distal hindlimb nerve conduction studies are feasible with surface recordings and stimulation. The evoked distal sensory potentials have amplitudes, configurations and recording techniques that are similar to humans and may be valuable for measuring large sensory fiber function in chronic models of peripheral neuropathies.
authors.affiliation: Department of Neurology and Neurological Sciences, Stanford University, Stanford, California, United States of America; California Institute for Medical Research, San Jose, California, United States of America.
authors.forename: John R
authors.initials: JR
authors.lastname: Hotson
mesh_terms: D000200:Action Potentials; D000818:Animals; D004195:Disease Models, Animal; D005071:Evoked Potentials; D005260:Female; D006614:Hindlimb / Q000294:innervation*; D009431:Neural Conduction; D010523:Peripheral Nervous System Diseases / Q000503:physiopathology*; D010543:Peroneal Nerve / Q000503:physiopathology*; D011817:Rabbits