title: Prognostic Role of 14F7 Mab Immunoreactivity against N-Glycolyl GM3 Ganglioside in Colon Cancer.
abstract: Purpose. To assess the prognostic role of 14F7 Mab immunoreactivity, against N-Glycolyl GM3 ganglioside, in patients with colon cancer (CC) and to evaluate the relationship between its expression and clinicopathological features. Methods. Paraffin-embedded specimens were retrospectively collected from 50 patients with CC operated between 2004 and 2008. 14F7 Mab staining was determined by immunohistochemistry technique and its relation with survival and clinicopathologic features was evaluated. Results. The reactivity of 14F7 Mab was detected in all cases. Most cases had high level of immunostaining (70%) that showed statistical correlation with TNM stage (P = 0.025). In univariate survival analysis, level of 14F7 Mab immunoreactivity (P = 0.0078), TNM Stage (P = 0.0007) and lymphovascular invasion (0.027) were significant prognostic factors for overall survival. Among these variables, level of 14F7 Mab immunoreactivity (HR = 0.268; 95% CI  0.078-0.920; P = 0.036) and TNM stage (HR = 0.249; 95% CI 0.066-0.932; P = 0.039) were independent prognostic factors on multivariate analysis. Conclusions. This study is the first approach on the prognostic significance of 14F7 Mab immunoreactivity in patients with colon adenocarcinoma and this assessment might be used in the prognostic estimate of CC, although further studies will be required to validate these findings.
authors.affiliation: Department of Basic Research and Immunology, National Institute of Oncology and Radiobiology, 29 and F Street Vedado, 10400 Havana, Cuba.
authors.forename: Tania
authors.identifier: 0000-0002-9644-0048
authors.initials: T
authors.lastname: Lahera
authors.affiliation: Department of Cell Biology and Tissues Banking, National Institute of Oncology and Radiobiology, 29 and F Street Vedado, 10400 Havana, Cuba.
authors.forename: Adanays
authors.initials: A
authors.lastname: Calvo
authors.affiliation: Department of Basic Research and Immunology, National Institute of Oncology and Radiobiology, 29 and F Street Vedado, 10400 Havana, Cuba.
authors.forename: Griselda
authors.initials: G
authors.lastname: Torres
authors.affiliation: Department of Pathology, Manuel Fajardo Hospital, Zapata and D Street Vedado, 10400 Havana, Cuba.
authors.forename: Charles E
authors.initials: CE
authors.lastname: Rengifo
authors.affiliation: Department of Pathology, National Institute of Oncology and Radiobiology, 29 and F Street Vedado, 10400 Havana, Cuba.
authors.forename: Santiago
authors.initials: S
authors.lastname: Quintero
authors.affiliation: Department of Basic Research and Immunology, National Institute of Oncology and Radiobiology, 29 and F Street Vedado, 10400 Havana, Cuba.
authors.forename: María Del Carmen
authors.initials: Mdel C
authors.lastname: Arango
authors.affiliation: Department of Surgery, National Institute of Oncology and Radiobiology, 29 and F Street Vedado, 10400 Havana, Cuba.
authors.forename: Debora
authors.initials: D
authors.lastname: Danta
authors.affiliation: Department of Surgery, National Institute of Oncology and Radiobiology, 29 and F Street Vedado, 10400 Havana, Cuba.
authors.forename: José M
authors.initials: JM
authors.lastname: Vázquez
authors.affiliation: Department of Cell Biology and Tissues Banking, National Institute of Oncology and Radiobiology, 29 and F Street Vedado, 10400 Havana, Cuba.
authors.forename: Xiomara
authors.initials: X
authors.lastname: Escobar
authors.affiliation: Research and Development Direction, Center of Molecular Immunology, 216 Street and 15 Avenue Atabey, Playa, P.O. Box 16040, 11600 Havana, Cuba.
authors.forename: Adriana
authors.initials: A
authors.lastname: Carr