title: The effect of gamma keratose on cell viability in vitro after thermal stress and the regulation of cell death pathway-specific gene expression.
abstract: When skin is thermally burned, transfer of heat energy into the skin results in the destruction of cells. Some of these cells are damaged but may be capable of self-repair and survival, thereby contributing to spontaneous healing of the wound. Keratin protein-based biomaterials have been suggested as potential treatments for burn injury. Isolation of cortical proteins from hair fibers results in an acid soluble fraction of keratin proteins referred to as "gamma" keratose. In the present study, treatment with this fraction dissolved in media was able to maintain cell viability after thermal stress in an in vitro model using primary mouse dermal fibroblasts. PCR array analysis demonstrated that gamma keratose treatment may assist in the survival and salvage of thermally stressed cells by maintaining their viability through regulation of cell death pathway-related genes. Gamma keratose may be a promising biomaterial for burn treatment that aids in spontaneous wound healing from viable tissue surrounding the burn.
authors.affiliation: Wake Forest Institute for Regenerative Medicine, Wake Forest School of Medicine, Medical Center Boulevard, Winston Salem, NC 27157, USA.
authors.forename: Deepika R
authors.initials: DR
authors.lastname: Poranki
authors.affiliation: Virginia Tech - Wake Forest School of Biomedical Engineering and Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA. Electronic address: mvandyke2013@gmail.com.
authors.forename: Mark E
authors.initials: ME
authors.lastname: Van Dyke
mesh_terms: D000818:Animals; D002056:Burns / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D002470:Cell Survival / Q000187:drug effects*; D002478:Cells, Cultured; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D005786:Gene Expression Regulation / Q000187:drug effects; D007633:Keratins / Q000302:isolation & purification / Q000627:therapeutic use*; D051379:Mice; D015398:Signal Transduction / Q000187:drug effects; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D007633:Keratins
keywords: Apoptosis; Autophagy; Burn; Keratin; Skin; Thermal