title: Analysis of microRNAs in patients with systemic lupus erythematosus, using Solexa deep sequencing.
abstract: OBJECTIVE
  Our aim in this study was to identify and examine the differential expression of microRNAs in patients with systemic lupus erythematosus (SLE).
  
  
  METHODS
  We employed high-quality, high-throughput Solexa sequencing to clone and identify microRNAs in SLE patients and a control group.
  
  
  RESULTS
  From the sequencing data, we identified numerous microRNAs displaying significantly different levels of expression in patients with SLE and in healthy controls. The 212 and 199 microRNAs were upregulated and downregulated, respectively. Only 61 novel microRNAs exhibited significantly different levels of expression in the two groups. The target genes of the novel microRNAs identified in the SLE group were found to have cell metabolism functions. We also analyzed the chromosomal locations of the microRNAs with high level of expression between the two groups. A profile comparison revealed that the majority of transcripts were expressed at a similar level. The functional classes of the most abundant microRNAs were equally represented on each chromosome.
  
  
  CONCLUSIONS
  We identified novel and known microRNAs significantly enhancing our understanding of the microRNA expression profiles of SLE patients. These data also provide insight into the function of microRNAs in SLE and provide new strategies for future therapies.
authors.affiliation: Second Clinical Medical College, Jinan University, Shenzhen People's Hospital , Shenzhen, Guangdong Province , China .
authors.forename: Wenbiao
authors.initials: W
authors.lastname: Chen
authors.forename: Kuibi
authors.initials: K
authors.lastname: Tan
authors.forename: Jianrong
authors.initials: J
authors.lastname: Huang
authors.forename: Xiangqi
authors.initials: X
authors.lastname: Yu
authors.forename: Wujian
authors.initials: W
authors.lastname: Peng
authors.forename: Yuyu
authors.initials: Y
authors.lastname: Chen
authors.forename: Xiaocong
authors.initials: X
authors.lastname: Lin
authors.forename: Deheng
authors.initials: D
authors.lastname: Chen
authors.forename: Yong
authors.initials: Y
authors.lastname: Dai
mesh_terms: D015536:Down-Regulation / Q000235:genetics; D020869:Gene Expression Profiling; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D011237:Predictive Value of Tests; D015854:Up-Regulation / Q000235:genetics
chemical_list: D035683:MicroRNAs