title: The sensitivity of Daphnia magna and Daphnia curvirostris to 10 veterinary antibacterials and to some of their binary mixtures.
abstract: Aim of this study was to evaluate the suitability of Daphnia curvirostris for the acute toxicity test usually performed on Daphnia magna, and to compare the sensitivity of the two species toward 10 antibacterials [enrofloxacin (EFX), ciprofloxacin(CPX), sulfaguanidine (SGD), sulfadiazine (SDZ), sulfamethazine (SMZ), sulfaquinoxaline (SQO), sulfaclozine (SCZ), sulfamerazine (SMA), sulfadimethoxine (SDM) and trimethoprim (TMP)] and some of their binary mixtures. Furthermore, a tentative prolonged-toxicity test (lasting 13d) was settled up in order to evidence toxic responses with drug concentrations that were uneffective in the classic 48h immobilization test. Results showed that D. curvirostris was more sensitive than D. magna to the majority of compounds (6 out of 10). Lowest 48h EC50s were obtained with EFX (4.3mgL(-1) in D. curvirostris) and SGD (6.2mgL(-1) in D. magna). The toxicity of paired compounds was always concentration-additive or less than concentration-additive. In the prolonged-toxicity test mortality and/or reproduction inhibition were constantly observed. It was concluded that: (1) D. curvirostris could be a suitable model for the evaluation of acute toxicity of antibacterials since its sensitivity was generally greater than that of D. magna; (2) the toxicity of EFX and SGD should be given special attention as the two compounds, in the prolonged test, showed to be active at concentrations of 0.9mgL(-1) and 2.5mgL(-1), respectively; (3) the concentration addition is usually a reasonable worst case estimation of the environmental impact of antibacterial mixtures.
authors.affiliation: Dep. of Comparative Biomedicine and Food Safety, University of Padua, Italy. Electronic address: mirco.dallabona@studenti.unipd.it.
authors.forename: Mirco
authors.initials: M
authors.lastname: Dalla Bona
authors.affiliation: Veterinary Practitioner, University of Padua, Italy.
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Di Leva
authors.affiliation: Dep. of Comparative Biomedicine and Food Safety, University of Padua, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: De Liguoro
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000633:toxicity*; D003621:Daphnia / Q000187:drug effects* / Q000502:physiology; D012098:Reproduction / Q000187:drug effects; D018675:Toxicity Tests; D019155:Veterinary Drugs / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D000900:Anti-Bacterial Agents; D019155:Veterinary Drugs; D014874:Water Pollutants, Chemical
keywords: Comparison; Daphnia curvirostris; Daphnia magna; Fluoroquinolones; Mixtures; Sulfonamides