title: Oxidored-nitro domain containing protein 1 (NOR1) expression suppresses slug/vimentin but not snail in nasopharyngeal carcinoma: Inhibition of EMT in vitro and in vivo in mice.
abstract: Oxidored-nitro domain containing protein 1 (NOR1) is a putative tumor suppressor gene. In this study, NOR1 expression was detected in NPC tissues and non-cancerous nasopharyngeal epithelium. The data showed that NOR1 protein was decreased in NPC tissues. Lost expression NOR1 protein was associated with poor overall and event-free survival of NPC patients. Overexpression of NOR1 in NPC cells resulted in a significant morphological change and decreased expression of epithelial-to-mesenchymal transition (EMT) mediators (e.g., slug and vimentin), but induced cytokeratin 13 expression. A nude mouse metastasis assay revealed that overexpression of NOR1 decreased NPC tumor cells metastasis capacity in vivo. Knockdown of NOR1 expression in HeLa cells was sufficient to abrogate epithelial traits and to enhance cell migration and invasion. Concomitant inhibition of slug or vimentin alleviated induction of EMT, migration or invasion by NOR1 siRNA in HeLa cells in vitro. In conclusion, the data from the current study suggest, for the first time, that NOR1 plays an important role in NPC in ex vivo, in vitro, and in vivo.
authors.affiliation: Hunan Provincial Tumor Hospital and The Tumor Hospital Affiliated to Xiangya School of Medicine, The Central South University, Changsha, Hunan 410013, China; Cancer Research Institute, Xiangya School of Medicine, The Central South University, Changsha 410078, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Cancer Research Institute, Xiangya School of Medicine, The Central South University, Changsha 410078, China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Li
authors.affiliation: Cancer Research Institute, Xiangya School of Medicine, The Central South University, Changsha 410078, China.
authors.forename: Wenling
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Cancer Research Institute, Xiangya School of Medicine, The Central South University, Changsha 410078, China.
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Li
authors.affiliation: Hunan Key Laboratory of Nonresolving Inflammation and Cancer, Changsha 410013, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Yi
authors.affiliation: Department of Laboratory Medicine and Pathology, Masonic Cancer Center, University of Minnesota, Minneapolis, MN 55455, USA.
authors.forename: Jianbo
authors.initials: J
authors.lastname: Yang
authors.affiliation: Hunan Provincial Tumor Hospital and The Tumor Hospital Affiliated to Xiangya School of Medicine, The Central South University, Changsha, Hunan 410013, China; Cancer Research Institute, Xiangya School of Medicine, The Central South University, Changsha 410078, China.
authors.forename: Zhaoyang
authors.initials: Z
authors.lastname: Zeng
authors.affiliation: Department of Laboratory Medicine and Pathology, Masonic Cancer Center, University of Minnesota, Minneapolis, MN 55455, USA; Minnesota Craniofacial Research Training Program, University of Minnesota School of Dentistry, Minneapolis, MN 55455, USA.
authors.forename: Leah E
authors.initials: LE
authors.lastname: Colvin Wanshura
authors.affiliation: Department of Laboratory Medicine and Pathology, Masonic Cancer Center, University of Minnesota, Minneapolis, MN 55455, USA.
authors.forename: James B
authors.initials: JB
authors.lastname: McCarthy
authors.affiliation: Department of Pathology, The Second Xiangya Hospital of Central South University, Changsha 410011, Hunan, China.
authors.forename: Songqing
authors.initials: S
authors.lastname: Fan
authors.affiliation: Hunan Provincial Tumor Hospital and The Tumor Hospital Affiliated to Xiangya School of Medicine, The Central South University, Changsha, Hunan 410013, China; Cancer Research Institute, Xiangya School of Medicine, The Central South University, Changsha 410078, China.
authors.forename: Pan
authors.initials: P
authors.lastname: Zheng
authors.affiliation: Hunan Provincial Tumor Hospital and The Tumor Hospital Affiliated to Xiangya School of Medicine, The Central South University, Changsha, Hunan 410013, China; Cancer Research Institute, Xiangya School of Medicine, The Central South University, Changsha 410078, China.
authors.forename: Shengnan
authors.initials: S
authors.lastname: Chen
authors.affiliation: Hunan Provincial Tumor Hospital and The Tumor Hospital Affiliated to Xiangya School of Medicine, The Central South University, Changsha, Hunan 410013, China; Cancer Research Institute, Xiangya School of Medicine, The Central South University, Changsha 410078, China. Electronic address: xiangbolin@csu.edu.cn.
authors.forename: Bo
authors.initials: B
authors.lastname: Xiang
authors.affiliation: Cancer Research Institute, Xiangya School of Medicine, The Central South University, Changsha 410078, China. Electronic address: ligy@xysm.net.
authors.forename: Guiyuan
authors.initials: G
authors.lastname: Li
mesh_terms: D000818:Animals; D002277:Carcinoma; D016022:Case-Control Studies; D002465:Cell Movement; D018572:Disease-Free Survival; D058750:Epithelial-Mesenchymal Transition; D006367:HeLa Cells; D064593:Heterografts; D006801:Humans; D053208:Kaplan-Meier Estimate; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008819:Mice, Nude; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D009361:Neoplasm Invasiveness; D011554:Pseudopodia / Q000378:metabolism / Q000473:pathology; D034622:RNA Interference; D015398:Signal Transduction; D000071250:Snail Family Transcription Factors; D022502:Stress Fibers / Q000378:metabolism / Q000473:pathology; D013997:Time Factors; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014162:Transfection; D014746:Vimentin / Q000235:genetics / Q000378:metabolism*
chemical_list: D026901:Membrane Transport Proteins; C479458:OSCP1 protein, human; C000604407:SNAI1 protein, human; C000604449:Snai2 protein, mouse; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors; D014746:Vimentin
keywords: Epithelial-to-mesenchymal transition; Metastasis; NOR1; Nasopharyngeal carcinoma; Tumor suppressor gene