title: Anti-inflammatory diterpenoids from the roots of Euphorbia ebracteolata.
abstract: Thirteen diterpenoids (1-13), including two new norditerpene lactones (1-2) and eight new rosane diterpenoids (3-10), were isolated from the roots of Euphorbia ebracteolata. The structures were determined by 1D and 2D NMR, HRESIMS, and electronic circular dichroism (ECD). The ECD-based empirical rule for α,β-unsaturated-γ-lactones was applied to determine the absolute configurations of 1 and 2. Compounds 7, 10, and 13 exhibited significant inhibition of nitric oxide production in RAW 264.7 lipopolysaccharide-induced macrophages, with IC50 values of 2.44, 2.76, and 1.02 μM, respectively.
authors.affiliation: Key Laboratory of Structure-Based Drug Design and Discovery, Ministry of Education, Shenyang Pharmaceutical University , Shenyang 110016, People's Republic of China.
authors.forename: Zhi-Guo
authors.initials: ZG
authors.lastname: Liu
authors.forename: Zhan-Lin
authors.initials: ZL
authors.lastname: Li
authors.forename: Jiao
authors.initials: J
authors.lastname: Bai
authors.forename: Da-Li
authors.initials: DL
authors.lastname: Meng
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.forename: Yue-Hu
authors.initials: YH
authors.lastname: Pei
authors.forename: Feng
authors.initials: F
authors.lastname: Zhao
authors.forename: Hui-Ming
authors.initials: HM
authors.lastname: Hua
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D028481:Euphorbia / Q000737:chemistry*; D020128:Inhibitory Concentration 50; D007783:Lactones / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects; D051379:Mice; D015394:Molecular Structure; D009569:Nitric Oxide / Q000096:biosynthesis; D019906:Nuclear Magnetic Resonance, Biomolecular; D018517:Plant Roots / Q000737:chemistry; D013237:Stereoisomerism
chemical_list: D000893:Anti-Inflammatory Agents; D004224:Diterpenes; D004365:Drugs, Chinese Herbal; D007783:Lactones; D008070:Lipopolysaccharides; D009569:Nitric Oxide