title: Synthetic ligand affinity chromatography purification of human serum albumin and related fusion proteins.
abstract: Synthetic ligand affinity adsorbents offer an efficient means for purification of biopharmaceuticals. Single isomer textile dye C.I. Reactive Blue and newer ligands developed by rational design and screening of chemical combinatorial libraries based on a triazine scaffold are routinely used for the capture and purification of these proteins from engineered recombinant expression systems. Here we describe methods for the purification of recombinant human serum albumin and related fusion proteins using synthetic ligand affinity adsorbents.
authors.affiliation: ProMetic BioSciences Ltd, Cambridge, UK.
authors.forename: Sharon
authors.initials: S
authors.lastname: Williams
authors.forename: Phil
authors.initials: P
authors.lastname: Morton
authors.forename: Dev
authors.initials: D
authors.lastname: Baines
mesh_terms: D002846:Chromatography, Affinity / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008024:Ligands; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification*; D012709:Serum Albumin / Q000302:isolation & purification*
chemical_list: D008024:Ligands; D011993:Recombinant Fusion Proteins; D012709:Serum Albumin