title: Suppression of allatotropin simulates reproductive diapause in the mosquito Culex pipiens.
abstract: The cessation of juvenile hormone (JH) production is a key endocrine event that halts ovarian development and hence initiates diapause in females of the mosquito, Culex pipiens. The shutdown in endocrine activity of the corpora allata (CA), the source of JH, was manifested in the smaller size of CA in females reared under short daylengths (diapause) compared to those reared under long daylengths (nondiapause), as well as in low expression of the mRNA encoding allatotropin, the neuropeptide that promotes JH biosynthesis in the CA. Genes encoding both allatotropin and allatostatin were identified in C. pipiens, but only expression levels of allatotropin differed in the two types of females. Knockdown of allatotropin mRNA using RNA interference in females programmed for nondiapause resulted in a cessation of ovarian development akin to diapause. This arrest in development could be reversed with an application of JH. Our results thus suggest that suppression of allatotropin is a critical link in regulating the shutdown of the CA during diapause.
authors.affiliation: Department of Biology, Baylor University, Waco, TX 76798, USA.
authors.forename: David S
authors.initials: DS
authors.lastname: Kang
authors.affiliation: Department of Evolution, Ecology, and Organismal Biology, Ohio State University, 318 West 12th Avenue, Columbus, OH 43210, USA; Department of Entomology, Ohio State University, 318 West 12th Avenue, Columbus, OH 43210, USA. Electronic address: denlinger.1@osu.edu.
authors.forename: David L
authors.initials: DL
authors.lastname: Denlinger
authors.affiliation: Department of Biology, Baylor University, Waco, TX 76798, USA. Electronic address: cheolho_sim@baylor.edu.
authors.forename: Cheolho
authors.initials: C
authors.lastname: Sim
mesh_terms: D000818:Animals; D001483:Base Sequence; D003335:Corpora Allata / Q000502:physiology; D003465:Culex / Q000502:physiology*; D064666:Diapause, Insect; D005260:Female; D007301:Insect Hormones / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D009032:Mosquito Control / Q000379:methods; D009479:Neuropeptides / Q000096:biosynthesis*; D010053:Ovary / Q000254:growth & development*; D017440:Photoperiod; D034622:RNA Interference; D012717:Sesquiterpenes
chemical_list: D007301:Insect Hormones; D009479:Neuropeptides; D012717:Sesquiterpenes; C076523:allatostatin; C050669:allatotropin; C036585:juvenile hormone III
keywords: Allatostatin; Allatotropin; Culex pipiens; Diapause; Juvenile hormone