title: Preparation of truncated orf virus entry fusion complex proteins by chemical synthesis.
abstract: Members of the Chordopoxvirinae subfamily possess an unusual 11 protein entry-fusion complex (EFC) that is highly conserved and present in all species. The mode of action of this EFC is unknown, and the interactions of the constituent proteins are uncharacterised. Here, we present the chemical synthesis of membrane domain truncated linear constructs of two EFC proteins in orf virus, ORFV036 and 049. By using Boc solid phase peptide synthesis and native chemical ligation methods, these truncated proteins have been readily prepared in milligram quantities. These robust synthetic protocols allow ready access to these polypeptides to facilitate biological studies.
authors.affiliation: School of Biological Sciences, University of Auckland, 3A Symonds Street, Auckland, New Zealand.
authors.forename: Ho
authors.initials: H
authors.lastname: Yeung
authors.forename: Paul W R
authors.initials: PW
authors.lastname: Harris
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Squire
authors.forename: Edward N
authors.initials: EN
authors.lastname: Baker
authors.forename: Margaret A
authors.initials: MA
authors.lastname: Brimble
mesh_terms: D015394:Molecular Structure; D009923:Orf virus / Q000737:chemistry*; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry; D014760:Viral Fusion Proteins / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D010455:Peptides; D014760:Viral Fusion Proteins
keywords: chordopoxvirus; entry fusion complex; native chemical ligation; orf virus