title: Structure of the O-polysaccharide of Photorhabdus temperata subsp. temperata XlNach(T) containing a novel branched monosaccharide, 3,6-dideoxy-4-C-[(S)-1,2-dihydroxyethyl]-d-xylo-hexose.
abstract: O-Polysaccharide was isolated from the lipopolysaccharide of an entomopathogenic bacterium Photorhabdus temperata subsp. temperata XlNach(T). Sugar analysis after full acid hydrolysis of the polysaccharide revealed D-glucose, D-mannose, D-galactose, D-GalNAc, and a branched monosaccharide, 3,6-dideoxy-4-C-[(S)-1',2'-dihydroxyethyl]-D-xylo-hexose (Sug), which was isolated as a 1,2'-anhydro furanose derivative. The following structure of the polysaccharide was established by 1D and 2D 1H and 13C NMR spectroscopy:
authors.affiliation: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Nikolay P
authors.initials: NP
authors.lastname: Arbatsky
authors.affiliation: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Shashkov
authors.affiliation: State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, Russia.
authors.forename: Nadezhda A
authors.initials: NA
authors.lastname: Kirsheva
authors.affiliation: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Anna N
authors.initials: AN
authors.lastname: Kondakova
authors.affiliation: State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, Russia.
authors.forename: Rima Z
authors.initials: RZ
authors.lastname: Shaikhutdinova
authors.affiliation: State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, Russia.
authors.forename: Sergey A
authors.initials: SA
authors.lastname: Ivanov
authors.affiliation: State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, Russia.
authors.forename: Andrey P
authors.initials: AP
authors.lastname: Anisimov
authors.affiliation: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia. Electronic address: yknirel@gmail.com.
authors.forename: Yuriy A
authors.initials: YA
authors.lastname: Knirel
mesh_terms: D002240:Carbohydrate Sequence; D006601:Hexoses / Q000737:chemistry*; D008969:Molecular Sequence Data; D020637:Photorhabdus / Q000737:chemistry*; D011135:Polysaccharides, Bacterial / Q000737:chemistry*
chemical_list: D006601:Hexoses; D011135:Polysaccharides, Bacterial
keywords: Bacterial polysaccharide structure; Branched monosaccharide; Lipopolysaccharide; O-Antigen; Photorhabdus temperata