title: Mitochondrial protein synthesis is increased in oxidative skeletal muscles of rats with cardiac cachexia.
abstract: Since cardiac cachexia could be associated with alterations in muscular mitochondrial metabolism, we hypothesized that the expected alterations in the activities of mitochondrial oxidative enzymes could be associated with changes in mitochondrial protein synthesis in oxidative skeletal muscles. Cardiac cachexia was provoked in male rats by the ligation of the left coronary artery. Six cachectic and 6 control rats were age-paired, and their food intake was observed. The synthesis of mitochondrial proteins was measured by [1-13C]-valine infusion in soleus, tibilais, myocardium, and liver. Muscles (soleus, gastrocnemius, and tibialis anterior), heart, kidneys, liver, and visceral adipose tissue were weighed. Mitochondrial cytochrome c oxydase IV as well as citrate synthase and myosin ATPase activities were measured. As expected, decreased food intake was observed in the cachectic group. Heart, kidney, and liver weights were higher in the cachectic group, while the visceral adipose tissue weight was lower (P < .01). No changes in muscle weights were observed. Soleus mitochondrial proteins fractional synthesis rate was higher in the cachectic group (P = .054). Cytochrome c oxydase IV activity was reduced (P = .009) and increased (P = .038) in the soleus and liver of the cachectic rats, respectively. No change in citrate synthase activity was observed. Myosin ATPase activity was reduced in the gastrocnemius of the cachectic group (P < .01). Mitochondrial protein synthesis is increased in the soleus of rats with cardiac cachexia, suggesting a compensatory mechanism of the impaired oxidative mitochondrial function. Further work should assess whether the mitochondrial protein synthesis is altered in chronic heart failure patients with cardiac cachexia, and whether this is the cause or the consequence of cachexia.
authors.affiliation: Clermont Université, Université d'Auvergne, Unité de Nutrition Humaine, BP 10448, F-63000 CLERMONT-FERRAND, Cedex, France; INRA, UMR 1019, UNH, CRNH Auvergne, F-63000 CLERMONT-FERRAND, Cedex, France; CHU Clermont-Ferrand, Service de Nutrition Clinique, F-63003 CLERMONT-FERRAND, Cedex, France; Nutrition Unit, Geneva University Hospital, Geneva, Switzerland.
authors.forename: Ronan
authors.initials: R
authors.lastname: Thibault
authors.affiliation: CHU Clermont-Ferrand, Service de Cardiologie, F-63003 CLERMONT-FERRAND, Cedex, France.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Chanséaume
authors.affiliation: CHU Clermont-Ferrand, Service de Chirurgie Vasculaire, F-63003 CLERMONT-FERRAND, Cedex, France.
authors.forename: Kasra
authors.initials: K
authors.lastname: Azarnoush
authors.affiliation: Clermont Université, Université d'Auvergne, Unité de Nutrition Humaine, BP 10448, F-63000 CLERMONT-FERRAND, Cedex, France; INRA, UMR 1019, UNH, CRNH Auvergne, F-63000 CLERMONT-FERRAND, Cedex, France.
authors.forename: Christelle
authors.initials: C
authors.lastname: Guillet
authors.affiliation: Clermont Université, Université d'Auvergne, Unité de Nutrition Humaine, BP 10448, F-63000 CLERMONT-FERRAND, Cedex, France; INRA, UMR 1019, UNH, CRNH Auvergne, F-63000 CLERMONT-FERRAND, Cedex, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Giraudet
authors.affiliation: Clermont Université, Université d'Auvergne, Unité de Nutrition Humaine, BP 10448, F-63000 CLERMONT-FERRAND, Cedex, France; INRA, UMR 1019, UNH, CRNH Auvergne, F-63000 CLERMONT-FERRAND, Cedex, France.
authors.forename: Véronique
authors.initials: V
authors.lastname: Patrac
authors.affiliation: Nutrition Unit, Geneva University Hospital, Geneva, Switzerland.
authors.forename: Jean-René
authors.initials: JR
authors.lastname: Lusson
authors.affiliation: Clermont Université, Université d'Auvergne, Unité de Nutrition Humaine, BP 10448, F-63000 CLERMONT-FERRAND, Cedex, France; INRA, UMR 1019, UNH, CRNH Auvergne, F-63000 CLERMONT-FERRAND, Cedex, France; CHU Clermont-Ferrand, Service de Nutrition Clinique, F-63003 CLERMONT-FERRAND, Cedex, France.
authors.forename: Noël
authors.initials: N
authors.lastname: Cano
authors.affiliation: Clermont Université, Université d'Auvergne, Unité de Nutrition Humaine, BP 10448, F-63000 CLERMONT-FERRAND, Cedex, France; INRA, UMR 1019, UNH, CRNH Auvergne, F-63000 CLERMONT-FERRAND, Cedex, France; CHU Clermont-Ferrand, Service de Nutrition Clinique, F-63003 CLERMONT-FERRAND, Cedex, France.
authors.forename: Yves
authors.initials: Y
authors.lastname: Boirie
authors.affiliation: Clermont Université, Université d'Auvergne, Unité de Nutrition Humaine, BP 10448, F-63000 CLERMONT-FERRAND, Cedex, France; INRA, UMR 1019, UNH, CRNH Auvergne, F-63000 CLERMONT-FERRAND, Cedex, France. Electronic address: stephane.walrand@clermont.inra.fr.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Walrand
mesh_terms: D000596:Amino Acids / Q000097:blood; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight; D002100:Cachexia / Q000473:pathology*; D002950:Citrate (si)-Synthase / Q000378:metabolism; D004452:Echocardiography; D003576:Electron Transport Complex IV / Q000378:metabolism; D006321:Heart / Q000503:physiopathology; D007328:Insulin / Q000097:blood; D007668:Kidney / Q000503:physiopathology; D019344:Lactic Acid / Q000097:blood; D050356:Lipid Metabolism; D008099:Liver / Q000503:physiopathology; D008297:Male; D008931:Mitochondria, Muscle / Q000201:enzymology; D024101:Mitochondrial Proteins / Q000096:biosynthesis*; D018482:Muscle, Skeletal / Q000503:physiopathology*; D009218:Myosins / Q000378:metabolism; D009929:Organ Size; D018384:Oxidative Stress; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D011917:Rats, Inbred Lew; D014280:Triglycerides / Q000097:blood; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism
chemical_list: D000596:Amino Acids; D001786:Blood Glucose; D007328:Insulin; D024101:Mitochondrial Proteins; D012333:RNA, Messenger; D014280:Triglycerides; D014409:Tumor Necrosis Factor-alpha; D019344:Lactic Acid; D003576:Electron Transport Complex IV; D002950:Citrate (si)-Synthase; D009218:Myosins
keywords: ATP; Cardiac cachexia; Coronary ligation; Enzymes; Heart failure; Myocardial infarction; Oxidation; Rat model