title: Convergent methods assessing bone growth in an experimental model at dental implants in the minipig.
abstract: Implant dentistry demonstrated its reliability in treating successfully an increased amount of patients with dental implants exhibiting hydrophilic (modSLA) or non-hydrophilic (SLA) surfaces. Objectives of this minipig study consisted in assessing bone regeneration at both types of dental implants in a new experimental model (lateral bone defects) by implementing a convergent analysis approach combining histology and contact radiography. In six adult female minipigs standardized acute alveolar defects were created, then receiving two implants in the mandibles bilaterally (modSLA and SLA, one of each combined with Guided Bone Regeneration). Animals were sacrificed after 28 days of healing. Mid-crestal specimen were analyzed assessing missing BIC to implant shoulder (fBIC); vertical bone growth upon implant surface, bone:tissue ratio and grafting material area occupied by material remnants for GBR sites. Values obtained from both analyses were compared and statistical correlations scrutinized. Although dimensions of mean differences and adjusted means given by radiographic method were lower than histological ones, comparison of different implants yielded similar results. Statistical analyses of correlation and concordance coefficients used to evaluate radiological method of measurement showed high level of concordance (concordance coefficient=0.912 and correlation coefficient=0.939) for fBIC. Similar results were observed for vertical new bone and for remnants of graft. Discrepancies for new bone and for mineralized tissue resulted in concordance coefficient of 0.182 and 0.054. The results indicate that contact X-rays can be used for morphometric assessments regarding defect fill; however, histological staining remains beneficial if greater resolution for distinguishing qualitative differences in the tissues is required.
authors.affiliation: Department of Periodontology, School of Dentistry, Faculty of Health, University of Witten, Alfred-Herrhausen-Str. 44, 58455 Witten, Germany. Electronic address: anton.friedmann@uni-wh.de.
authors.forename: Anton
authors.initials: A
authors.lastname: Friedmann
authors.affiliation: Department of Periodontology, School of Dentistry, Faculty of Health, University of Witten, Alfred-Herrhausen-Str. 44, 58455 Witten, Germany.
authors.forename: Asisa
authors.initials: A
authors.lastname: Friedmann
authors.affiliation: Department of Epidemiology and Public Health, Swiss Tropical and Public Health Institute, Basel, Switzerland; University of Basel, Basel, Switzerland.
authors.forename: Leticia
authors.initials: L
authors.lastname: Grize
authors.affiliation: Institute Staumann AG, Basel, Switzerland.
authors.forename: Marcel
authors.initials: M
authors.lastname: Obrecht
authors.affiliation: Institute Staumann AG, Basel, Switzerland; New York University, College of Dentistry, NY, USA.
authors.forename: Michel
authors.initials: M
authors.lastname: Dard
mesh_terms: D016301:Alveolar Bone Loss / Q000000981:diagnostic imaging / Q000473:pathology; D000539:Alveolar Process / Q000000981:diagnostic imaging / Q000473:pathology; D000818:Animals; D001706:Biopsy; D001846:Bone Development / Q000502:physiology*; D016025:Bone Transplantation; D003758:Dental Implantation, Endosseous; D015921:Dental Implants; D005260:Female; D008954:Models, Biological; D011859:Radiography; D013552:Swine; D013556:Swine, Miniature / Q000033:anatomy & histology*
chemical_list: D015921:Dental Implants
keywords: Animal trial; Concordance coefficient; Contact radiography; GBR; Histomorphometry; SLA and modSLA implants