title: Histopathology of motor cortex in an experimental focal ischemic stroke in mouse model.
abstract: Experimental ischemia results in cortical brain lesion followed by ischemic stroke. In this study, focal cerebral ischemia was induced in mice by occlusion of the middle cerebral artery. We studied cortical layers I, II/III, V and VI in the caudal forelimb area (CFA) and medial agranular cortex (AGm) from control and C57BL/6 mice induced with ischemic stroke. Based on our analysis of CFA and AGm motor cortex, significant differences were observed in the numbers of neurons, astrocytes and microglia in the superficial II/III and deep V cortical layers. Cellular changes were more prominent in layer V of the CFA with nuclear pyknosis, chromatin fragmentation, necrosis and degeneration, as well as, morphological evidence of apoptosis, mainly in neurons. As result, the CFA was more severely impaired than the AGm in this focal cerebral ischemic model, as evidenced by the proliferation of astrocytes, potentially resulting in neuroinflammation by microglia-like cells.
authors.affiliation: Departamento de Ciências Morfológicas, Universidade Federal Santa Catarina, Brazil.
authors.forename: Juçara Loli
authors.initials: JL
authors.lastname: de Oliveira
authors.affiliation: Departamento de Matemática, Universidade Federal de Rondônia, Brazil.
authors.forename: Pedro di Tárique Barreto
authors.initials: Pd
authors.lastname: Crispin
authors.affiliation: Departamento de Ciências Morfológicas, Universidade Federal Santa Catarina, Brazil.
authors.forename: Elisa Cristiana Winkelmann
authors.initials: EC
authors.lastname: Duarte
authors.affiliation: Departamento de Ciências Morfológicas, Universidade Federal Santa Catarina, Brazil.
authors.forename: Gilberto Domingos
authors.initials: GD
authors.lastname: Marloch
authors.affiliation: Departamento de Biologia Celular, Embriologia e Genética, Universidade Federal de Santa Catarina, Brazil.
authors.forename: Rogério
authors.initials: R
authors.lastname: Gargioni
authors.affiliation: Departamento de Biologia Celular, Embriologia e Genética, Universidade Federal de Santa Catarina, Brazil.
authors.forename: Andréa Gonçalves
authors.initials: AG
authors.lastname: Trentin
authors.affiliation: Departamento de Biologia Celular, Embriologia e Genética, Universidade Federal de Santa Catarina, Brazil. Electronic address: marcio.alvarez@ufsc.br.
authors.forename: Marcio
authors.initials: M
authors.lastname: Alvarez-Silva
mesh_terms: D000818:Animals; D017209:Apoptosis; D002545:Brain Ischemia / Q000473:pathology*; D053148:Caspase 3 / Q000378:metabolism; D002452:Cell Count; D005552:Forelimb / Q000294:innervation; D007150:Immunohistochemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D009044:Motor Cortex / Q000473:pathology*; D009336:Necrosis; D020521:Stroke / Q000473:pathology*
chemical_list: D053148:Caspase 3
keywords: Apoptosis; Cerebral ischemia; Cortex cytoarchitecture; Motor cortex; Necrosis; Neuroinflammation