title: Protein kinase Cθ gene expression is oppositely regulated by GCN5 and EBF1 in immature B cells.
abstract: In this study, we revealed that GCN5 and early B cell factor 1 (EBF1) participate in regulation of protein kinase Cθ (PKCθ) gene expression in an opposite manner in immature B cells. GCN5-deficiency in DT40 caused drastic down-regulation of transcription of PKCθ. In contrast, EBF1-deficiency brought about remarkable up-regulation of that of PKCθ, and re-expression of EBF1 dramatically suppressed transcription of PKCθ. Chromatin immunoprecipitation assay revealed that GCN5 binds to the 5'-flanking region of the chicken PKCθ gene and acetylates histone H3, and EBF1 binds to the 5'-flanking region of the gene surrounding putative EBF1 binding motifs.
authors.affiliation: Section of Biochemistry and Molecular Biology, Department of Medical Sciences, Faculty of Medicine, University of Miyazaki, 5200, Kihara, Kiyotake, Miyazaki 889-1692, Japan; Laboratory Center for Proteomics Research, Graduate School of Frontier Sciences, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Tokyo 108-8639, Japan. Electronic address: masakari@med.miyazaki-u.ac.jp.
authors.forename: Hidehiko
authors.initials: H
authors.lastname: Kikuchi
authors.affiliation: Section of Biochemistry and Molecular Biology, Department of Medical Sciences, Faculty of Medicine, University of Miyazaki, 5200, Kihara, Kiyotake, Miyazaki 889-1692, Japan.
authors.forename: Masami
authors.initials: M
authors.lastname: Nakayama
authors.affiliation: Laboratory Center for Proteomics Research, Graduate School of Frontier Sciences, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Tokyo 108-8639, Japan; Department of Biochemistry, Kawasaki Medical School, 577, Matsushima, Kurashiki, Okayama 701-0192, Japan.
authors.forename: Futoshi
authors.initials: F
authors.lastname: Kuribayashi
authors.affiliation: Laboratory Center for Proteomics Research, Graduate School of Frontier Sciences, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Tokyo 108-8639, Japan.
authors.forename: Shinobu
authors.initials: S
authors.lastname: Imajoh-Ohmi
authors.affiliation: Section of Biochemistry and Molecular Biology, Department of Medical Sciences, Faculty of Medicine, University of Miyazaki, 5200, Kihara, Kiyotake, Miyazaki 889-1692, Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Nishitoh
authors.affiliation: Section of Biochemistry and Molecular Biology, Department of Medical Sciences, Faculty of Medicine, University of Miyazaki, 5200, Kihara, Kiyotake, Miyazaki 889-1692, Japan.
authors.forename: Yasunari
authors.initials: Y
authors.lastname: Takami
authors.affiliation: Section of Biochemistry and Molecular Biology, Department of Medical Sciences, Faculty of Medicine, University of Miyazaki, 5200, Kihara, Kiyotake, Miyazaki 889-1692, Japan.
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Nakayama
mesh_terms: D024506:5' Flanking Region; D000107:Acetylation; D000818:Animals; D030161:Avian Proteins / Q000235:genetics* / Q000378:metabolism; D001402:B-Lymphocytes / Q000201:enzymology*; D001483:Base Sequence; D001665:Binding Sites; D002478:Cells, Cultured; D002645:Chickens; D015536:Down-Regulation; D004794:Enzyme Repression; D051548:Histone Acetyltransferases / Q000502:physiology*; D006657:Histones / Q000378:metabolism; D008969:Molecular Sequence Data; D011485:Protein Binding; D011493:Protein Kinase C / Q000235:genetics* / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015534:Trans-Activators / Q000502:physiology*
chemical_list: D030161:Avian Proteins; D006657:Histones; D012333:RNA, Messenger; D015534:Trans-Activators; D051548:Histone Acetyltransferases; D011493:Protein Kinase C
keywords: DT40; Early B cell factor 1; GCN5; Gene targeting; PKCθ