title: MicroRNA-21 regulates biological behaviors in papillary thyroid carcinoma by targeting programmed cell death 4.
abstract: BACKGROUND
  Our recent study has found that microRNA-21 (miRNA-21) was significantly upregulated in papillary thyroid carcinoma (PTC) tissues compared with nontumor tissues by using miRNA microarray chip. However, the function of miRNA-21 is unknown in PTC. The aim of this study was to investigate the roles of miRNA-21 in PTC and the mechanism of gene regulation by it.
  
  
  METHODS
  We transfected PTC cell line (TPC-1) with pEZX-eGFP-miRNA-21 plasmid to determine the biological functions of miRNA-21. Western blot assay was applied to investigate the correlation between miRNA-21 and programmed cell death 4 (PDCD4) expression in TPC-1 cell line.
  
  
  RESULTS
  Overexpression of miRNA-21 could significantly enhance proliferation and invasion and inhibit the apoptosis of TPC-1 cells. In addition, miRNA-21 and PDCD4 expression showed a significantly negative correlation in TPC-1 cells.
  
  
  CONCLUSIONS
  These data suggest that miRNA-21 may play an oncogenic role by directly targeting PDCD4 in the cellular processes of PTC. In addition, the findings in our present study also may represent new clues for the diagnostic and therapeutic strategies in the treatment of PTC.
authors.affiliation: Department of Thyroid Surgery, the First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan, China; Department of Pediatric Endocrinology, the First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Thoracic surgery, the First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Thyroid Surgery, the First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Thyroid Surgery, the First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan, China.
authors.forename: Yuxia
authors.initials: Y
authors.lastname: Fan
authors.affiliation: Department of Thyroid Surgery, the First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Thyroid Surgery, the First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan, China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Thyroid Surgery, the First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan, China.
authors.forename: Qingling
authors.initials: Q
authors.lastname: Yuan
authors.affiliation: Department of Thyroid Surgery, the First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan, China.
authors.forename: Yixing
authors.initials: Y
authors.lastname: Yin
authors.affiliation: Department of Thyroid Surgery, the First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Yu
authors.affiliation: Department of Thyroid Surgery, the First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan, China.
authors.forename: Meiju
authors.initials: M
authors.lastname: Zhu
authors.affiliation: Department of Thyroid Surgery, the First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zheng
authors.affiliation: Department of Thyroid Surgery, the First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan, China. Electronic address: luxiubo68877@126.com.
authors.forename: Xiubo
authors.initials: X
authors.lastname: Lu
mesh_terms: D017209:Apoptosis / Q000235:genetics; D051017:Apoptosis Regulatory Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000235:genetics; D002277:Carcinoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D002291:Carcinoma, Papillary; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation / Q000235:genetics; D006801:Humans; D035683:MicroRNAs / Q000502:physiology*; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000473:pathology; D016601:RNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D000077273:Thyroid Cancer, Papillary; D013964:Thyroid Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology
chemical_list: D051017:Apoptosis Regulatory Proteins; D014408:Biomarkers, Tumor; C524940:MIRN21 microRNA, human; D035683:MicroRNAs; C114487:PDCD4 protein, human; D016601:RNA-Binding Proteins
keywords: MicroRNA-21; Papillary thyroid carcinoma; Programmed cell death 4; TPC-1