title: Quality and antitumour activity evaluation of extract of Hypericum ascyron.
abstract: Similarity assessment of complex chromatographic profiles is a potential tool for the identification and quality control of herbal medicinal products to guarantee the expected biological activity. In this paper, a high-performance liquid chromatography method was established for controlling the quality of extract of Hypericum ascyron for the first time. With this method, the correlation coefficients of similarity of 10 batches extract of H. ascyron were >0.97. The extract of H. ascyron displayed steadily inhibitorty activities on the growth of human cervical cancer Hela cell lines. Therefore, the present study successfully set up a sensitive efficient method which might confirm stable biological activity of the extract of H. ascyron.
authors.affiliation: Key Laboratory of Industrial Fermentation Microbiology of Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, People's Republic of China.
authors.forename: Xiu-Mei
authors.initials: XM
authors.lastname: Li
authors.forename: Xue-Gang
authors.initials: XG
authors.lastname: Luo
authors.forename: Ning
authors.initials: N
authors.lastname: Ma
authors.forename: Kun
authors.initials: K
authors.lastname: Li
authors.forename: Wen
authors.initials: W
authors.lastname: Li
authors.forename: De-Yun
authors.initials: DY
authors.lastname: Ma
authors.forename: Tong-Cun
authors.initials: TC
authors.lastname: Zhang
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D004354:Drug Screening Assays, Antitumor; D004355:Drug Stability; D006367:HeLa Cells; D006801:Humans; D020902:Hypericum / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry*; D015203:Reproducibility of Results
chemical_list: D000970:Antineoplastic Agents; D010936:Plant Extracts
keywords: Hypericum ascyron; antitumor; extract; high-performance liquid chromatography; similarity analysis