title: Effects of dietary selenium on the pathological changes and oxidative stress in loach (Paramisgurnus dabryanus).
abstract: In this study, loach (Paramisgurnus dabryanus) were fed artificial diets containing 0.31 (control), 0.39, 0.48, 0.50 and 0.62 mg kg(-1) of selenium (Se) for 60 days, respectively. Liver histopathology, hepatocyte ultrastructure, blood indices, biochemical parameters of liver functions and oxidative stress in the Se-treated loach were then assayed. The results showed the following: histopathological and ultrastructural lesions in liver were only observed in loach fed the 0.62 mg Se kg(-1) diet; Haemoglobin and total protein were significantly increased in the 0.50 mg Se kg(-1) group; albumin and high-density lipoprotein were increased significantly in the 0.48-0.50 mg Se kg(-1) groups. However, white blood cell count was significantly decreased in the 0.48 mg Se kg(-1) group; alanine aminotransferase, aspartate aminotransferase and lactate dehydrogenase were decreased in the 0.39-0.50 mg Se kg(-1) groups. In liver tissue, the content of hydrogen peroxide was lower than that of controls in the 0.48-0.50 mg Se kg(-1) groups, and the malondialdehyde level was lowest in the 0.48 mg Se kg(-1) group. The activities of superoxide dismutase and glutathione peroxidase were significantly increased in the 0.50 mg Se kg(-1) group; catalase and total antioxidant capacity were markedly increased in the 0.48-0.50 mg Se kg(-1) group. These present results indicated that the dietary Se requirement for loach is 0.48-0.50 mg Se kg(-1) diet.
authors.affiliation: School of Preclinical Medicine and Life Science, Soochow University, 199, Renai Road, Suzhou, 215123, Jiangsu, People's Republic of China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Hao
authors.forename: Qufei
authors.initials: Q
authors.lastname: Ling
authors.forename: Fashui
authors.initials: F
authors.lastname: Hong
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000704:Analysis of Variance; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D003531:Cypriniformes / Q000502:physiology*; D019587:Dietary Supplements; D004305:Dose-Response Relationship, Drug; D006454:Hemoglobins / Q000378:metabolism; D022781:Hepatocytes / Q000648:ultrastructure; D007770:L-Lactate Dehydrogenase / Q000097:blood; D007958:Leukocyte Count / Q000662:veterinary; D008099:Liver / Q000187:drug effects / Q000473:pathology; D009751:Nutritional Requirements; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D011506:Proteins / Q000032:analysis; D012643:Selenium / Q000009:adverse effects / Q000494:pharmacology*
chemical_list: D006454:Hemoglobins; D011506:Proteins; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D012643:Selenium