title: Immunological evaluation of mannosylated chitosan nanoparticles based foot and mouth disease virus DNA vaccine, pVAC FMDV VP1-OmpA in guinea pigs.
abstract: A DNA vaccine for foot and mouth disease (FMD) based on mannosylated chitosan nanoparticles was evaluated in guinea pigs. The DNA construct was comprised of FMD virus full length-VP1 gene and outer membrane protein A (Omp A) gene of Salmonella typhimurium as a Toll-like receptor (TLR)-ligand in pVAC vector. Groups of guinea pigs immunized either intramuscularly or intra-nasally were evaluated for induction of virus neutralizing antibodies, Th1(IgG2) and Th2 (IgG1) responses, lymphocyte proliferation, reactive nitrogen intermediate production, secretory IgA for naso-mucosal immune response and protection upon homotypic type O virulent FMD virus challenge. The results indicate the synergistic effect of OmpA on the immunogenic potential of FMD DNA vaccine construct delivered using mannosylated chitosan nano-particles by different routes of administration. These observations suggest the substantial improvement in all the immunological parameters with enhanced protection in guinea pigs.
authors.affiliation: FMD Quality Control and Quality Assurance Laboratory, Indian Veterinary Research Institute, H A Farm, Hebbal, Bangalore 560 024, Karnataka, India.
authors.forename: Raj Kishore
authors.initials: RK
authors.lastname: Nanda
authors.affiliation: FMD Quality Control and Quality Assurance Laboratory, Indian Veterinary Research Institute, H A Farm, Hebbal, Bangalore 560 024, Karnataka, India.
authors.forename: Irshad Ahmed
authors.initials: IA
authors.lastname: Hajam
authors.affiliation: FMD Quality Control and Quality Assurance Laboratory, Indian Veterinary Research Institute, H A Farm, Hebbal, Bangalore 560 024, Karnataka, India.
authors.forename: Bedaso Mammo
authors.initials: BM
authors.lastname: Edao
authors.affiliation: FMD Quality Control and Quality Assurance Laboratory, Indian Veterinary Research Institute, H A Farm, Hebbal, Bangalore 560 024, Karnataka, India.
authors.forename: Kalaivanan
authors.initials: K
authors.lastname: Ramya
authors.affiliation: FMD Quality Control and Quality Assurance Laboratory, Indian Veterinary Research Institute, H A Farm, Hebbal, Bangalore 560 024, Karnataka, India.
authors.forename: Mageswary
authors.initials: M
authors.lastname: Rajangam
authors.affiliation: FMD Quality Control and Quality Assurance Laboratory, Indian Veterinary Research Institute, H A Farm, Hebbal, Bangalore 560 024, Karnataka, India.
authors.forename: Shanmugam
authors.initials: S
authors.lastname: Chandra Sekar
authors.affiliation: FMD Quality Control and Quality Assurance Laboratory, Indian Veterinary Research Institute, H A Farm, Hebbal, Bangalore 560 024, Karnataka, India.
authors.forename: Kondabattula
authors.initials: K
authors.lastname: Ganesh
authors.affiliation: FMD Quality Control and Quality Assurance Laboratory, Indian Veterinary Research Institute, H A Farm, Hebbal, Bangalore 560 024, Karnataka, India.
authors.forename: Veerakyathappa
authors.initials: V
authors.lastname: Bhanuprakash
authors.affiliation: FMD Quality Control and Quality Assurance Laboratory, Indian Veterinary Research Institute, H A Farm, Hebbal, Bangalore 560 024, Karnataka, India. Electronic address: subodhkishore@gmail.com.
authors.forename: Subodh
authors.initials: S
authors.lastname: Kishore
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D002460:Cell Line; D048271:Chitosan / Q000737:chemistry*; D006224:Cricetinae; D004797:Enzyme-Linked Immunosorbent Assay; D005536:Foot-and-Mouth Disease / Q000276:immunology / Q000517:prevention & control*; D006168:Guinea Pigs; D007111:Immunity, Cellular; D008358:Mannose / Q000737:chemistry*; D053758:Nanoparticles; D019444:Vaccines, DNA / Q000737:chemistry / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D019444:Vaccines, DNA; D048271:Chitosan; D008358:Mannose
keywords: ELISA; FMD DNA vaccine; Mannosylated chitosan nanoparticles delivery; OmpA; Protection; SNT; Salmonella typhimurium