title: Drosophila melanogaster as a model system to study long-chain fatty acid amide metabolism.
abstract: Long-chain fatty acid amides are cell-signaling lipids identified in mammals and, recently, in invertebrates, as well. Many details regarding fatty acid amide metabolism remain unclear. Herein, we demonstrate that Drosophila melanogaster is an excellent model system for the study long-chain fatty acid amide metabolism as we have quantified the endogenous levels of N-acylglycines, N-acyldopamines, N-acylethanolamines, and primary fatty acid amides by LC/QTOF-MS. Growth of D. melanogaster on media supplemented with [1-(13)C]-palmitate lead to a family of (13)C-palmitate-labeled fatty acid amides in the fly heads. The [1-(13)C]-palmitate feeding studies provide insight into the biosynthesis of the fatty acid amides.
authors.affiliation: Department of Chemistry, University of South Florida, Tampa, FL 33620, USA.
authors.forename: Kristen A
authors.initials: KA
authors.lastname: Jeffries
authors.affiliation: Department of Chemistry, University of South Florida, Tampa, FL 33620, USA.
authors.forename: Daniel R
authors.initials: DR
authors.lastname: Dempsey
authors.affiliation: Department of Chemistry, University of South Florida, Tampa, FL 33620, USA.
authors.forename: Anita L
authors.initials: AL
authors.lastname: Behari
authors.affiliation: Department of Chemistry, University of South Florida, Tampa, FL 33620, USA.
authors.forename: Ryan L
authors.initials: RL
authors.lastname: Anderson
authors.affiliation: Department of Chemistry, University of South Florida, Tampa, FL 33620, USA. Electronic address: merkler@usf.edu.
authors.forename: David J
authors.initials: DJ
authors.lastname: Merkler
mesh_terms: D000818:Animals; D004331:Drosophila melanogaster / Q000378:metabolism*; D004983:Ethanolamines / Q000378:metabolism; D050356:Lipid Metabolism; D010169:Palmitic Acids / Q000378:metabolism
chemical_list: D004983:Ethanolamines; D010169:Palmitic Acids
keywords: Biosynthetic pathway; Drosophila melanogaster; Endogenous quantification; Heavy-labeled precursor; Long-chain fatty acid amide; Model system