title: Organic loading rate impact on biohydrogen production and microbial communities at anaerobic fluidized thermophilic bed reactors treating sugarcane stillage.
abstract: This study aimed to evaluate the effect of high organic loading rates (OLR) (60.0-480.00 kg COD m(-3)d(-1)) on biohydrogen production at 55°C, from sugarcane stillage for 15,000 and 20,000 mg CODL(-1), in two anaerobic fluidized bed reactors (AFBR1 and AFBR2). It was obtained, for H2 yield and content, a decreasing trend by increasing the OLR. The maximum H2 yield was observed in AFBR1 (2.23 mmol g COD added(-1)). The volumetric H2 production was proportionally related to the applied hydraulic retention time (HRT) of 6, 4, 2 and 1h and verified in AFBR1 the highest value (1.49 L H2 h(-1)L(-1)). Among the organic acids obtained, there was a predominance of lactic acid (7.5-22.5%) and butyric acid (9.4-23.8%). The microbial population was set with hydrogen-producing fermenters (Megasphaera sp.) and other organisms (Lactobacillus sp.).
authors.affiliation: Department of Hydraulic and Sanitation, University of São Paulo, Av. Trabalhador Sãocarlense, 400, Centro, CEP 13566-590, São Carlos, SP, Brazil.
authors.forename: Samantha Christine
authors.initials: SC
authors.lastname: Santos
authors.affiliation: Department of Chemical Engineering, Federal University of São Carlos, Rod. Washington Luis, km 235, CEP 13565-905, São Carlos, SP, Brazil.
authors.forename: Paula Rúbia Ferreira
authors.initials: PR
authors.lastname: Rosa
authors.affiliation: Department of Hydraulic and Sanitation, University of São Paulo, Av. Trabalhador Sãocarlense, 400, Centro, CEP 13566-590, São Carlos, SP, Brazil.
authors.forename: Isabel Kimiko
authors.initials: IK
authors.lastname: Sakamoto
authors.affiliation: Department of Hydraulic and Sanitation, University of São Paulo, Av. Trabalhador Sãocarlense, 400, Centro, CEP 13566-590, São Carlos, SP, Brazil.
authors.forename: Maria Bernadete Amâncio
authors.initials: MB
authors.lastname: Varesche
authors.affiliation: Department of Chemical Engineering, Federal University of São Carlos, Rod. Washington Luis, km 235, CEP 13565-905, São Carlos, SP, Brazil. Electronic address: edsilva@ufscar.br.
authors.forename: Edson Luiz
authors.initials: EL
authors.lastname: Silva
mesh_terms: D000693:Anaerobiosis / Q000187:drug effects; D001419:Bacteria / Q000187:drug effects / Q000378:metabolism*; D044822:Biodiversity; D018441:Biofilms / Q000187:drug effects; D056804:Biofuels / Q000382:microbiology*; D057919:Biological Oxygen Demand Analysis; D019149:Bioreactors / Q000382:microbiology*; D001709:Biotechnology / Q000295:instrumentation / Q000379:methods*; D002241:Carbohydrates / Q000302:isolation & purification; D006859:Hydrogen / Q000378:metabolism*; D009584:Nitrogen / Q000032:analysis; D009930:Organic Chemicals / Q000494:pharmacology*; D010758:Phosphorus / Q000032:analysis; D010802:Phylogeny; D031786:Saccharum / Q000187:drug effects*; D013431:Sulfates / Q000032:analysis; D013997:Time Factors; D014865:Waste Disposal, Fluid
chemical_list: D056804:Biofuels; D002241:Carbohydrates; D009930:Organic Chemicals; D013431:Sulfates; D010758:Phosphorus; D006859:Hydrogen; D009584:Nitrogen
keywords: Lactic acid; Lactobacillus sp.; Megasphaera elsdenii; Organic overload