title: Topical anti-inflammatory activity of a monofloral honey of Mimosa scabrella provided by Melipona marginata during winter in southern Brazil.
abstract: Melipona marginata is an endangered species of stingless bee from Brazil that produces honey with particular physicochemical features and a remarkable exotic flavor. To the best of our knowledge, this is the first report devoted to exploring the medicinal potential of this honey. Thus, the aim of this paper was to investigate the potential anti-inflammatory activity of honey extract from M. marginata on skin inflammation. The honey sample was classified as a monofloral honey of Mimosa scabrella. The presence of 11 phenolic compounds as kaempferol and caffeic acid was detected using the high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (HPLC-UV-ESI-MS) method. The anti-inflammatory activity was measured using a 12-O-tetradecanoylphorbol-13-acetate-induced ear edema model of inflammation in mice. The topical application of the M. marginata honey extract (1.0 mg/ear) was able to reduce ear edema with an inhibitory effect of 54 ± 5%. This extract decreased the myeloperoxidase activity in 75 ± 3%, which suggests a lower leucocyte infiltration that was confirmed by histological analysis. This extract also provided a reduction of 55 ± 14% in the production of reactive oxygen species. This anti-inflammatory activity could be due to a synergic effect of the phenolic compounds identified in the honey sample. Taken together, these results open up new possibilities for the use of M. marginata honey extract in skin disorders.
authors.affiliation: 1 Department of Pharmacy, Federal University of Paraná , Curitiba, Brazil .
authors.forename: Débora M
authors.initials: DM
authors.lastname: Borsato
authors.forename: Arthur S
authors.initials: AS
authors.lastname: Prudente
authors.forename: Patrícia M
authors.initials: PM
authors.lastname: Döll-Boscardin
authors.forename: Aurélio V
authors.initials: AV
authors.lastname: Borsato
authors.forename: Cynthia F P
authors.initials: CF
authors.lastname: Luz
authors.forename: Beatriz H L N S
authors.initials: BH
authors.lastname: Maia
authors.forename: Daniela A
authors.initials: DA
authors.lastname: Cabrini
authors.forename: Michel F
authors.initials: MF
authors.lastname: Otuki
authors.forename: Marilis D
authors.initials: MD
authors.lastname: Miguel
authors.forename: Paulo V
authors.initials: PV
authors.lastname: Farago
authors.forename: Obdulio G
authors.initials: OG
authors.lastname: Miguel
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001516:Bees; D001938:Brazil; D002851:Chromatography, High Pressure Liquid; D003879:Dermatologic Agents / Q000494:pharmacology*; D004195:Disease Models, Animal; D004487:Edema / Q000188:drug therapy; D006722:Honey / Q000032:analysis*; D008297:Male; D051379:Mice; D031298:Mimosa / Q000737:chemistry*; D018384:Oxidative Stress / Q000187:drug effects; D009195:Peroxidase / Q000378:metabolism; D010636:Phenols / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism; D012871:Skin Diseases / Q000188:drug therapy; D053719:Tandem Mass Spectrometry; D013755:Tetradecanoylphorbol Acetate / Q000633:toxicity
chemical_list: D000893:Anti-Inflammatory Agents; D003879:Dermatologic Agents; D010636:Phenols; D017382:Reactive Oxygen Species; D009195:Peroxidase; D013755:Tetradecanoylphorbol Acetate
keywords: HPLC-UV-ESI-MS analysis; Mimosa scabrella; ear edema; monofloral honey; phenolic compounds; pollen analysis; stingless bee