title: From target discovery to clinical trials with armed antibody products.
abstract: Conventional chemotherapy of serious conditions (e.g., cancer and chronic inflammatory diseases) relies on the use of potent bioactive agents, which do not preferentially localize at the site of disease and which may harm healthy tissues. Intense pharmaceutical research efforts are being devoted to the development of targeted therapeutic agents, capable of selectively homing to diseased tissues, while sparing normal body structures. Biological mass spectrometry and chemical proteomics have revolutionized the way targets for ligand-based pharmacodelivery applications are discovered. In this article, we present a personal account on research activities in the field for the last decade, outlining our experience in the discovery of accessible biomarkers and in the development of potent targeted therapeutic agents.
  
  
  CONCLUSIONS
  The present review discusses evolution of proteomic methodologies applied to the discovery of new targets for therapeutic intervention in cancer and inflammatory diseases. Chemical proteomics-driven target discovery allowed the development of new classes of antibody-based targeting biologics, which are having an impact in the oncological and chronic inflammation clinical research. This article is part of a Special Issue entitled: 20years of Proteomics in memory of Viatliano Pallini. Guest Editors: Luca Bini, Juan J. Calvete, Natacha Turck, Denis Hochstrasser and Jean-Charles Sanchez.
authors.affiliation: Philochem AG, Libernstrasse 3, CH-8112 Otelfingen, Switzerland.
authors.forename: Giuliano
authors.initials: G
authors.lastname: Elia
authors.affiliation: Philochem AG, Libernstrasse 3, CH-8112 Otelfingen, Switzerland.
authors.forename: Tim
authors.initials: T
authors.lastname: Fugmann
authors.affiliation: Department of Chemistry and Applied BioSciences, Swiss Federal Institute of Technology (ETH Zürich), Wolfgang-Pauli-Strasse 10, CH-8093 Zurich, Switzerland. Electronic address: dario.neri@pharma.ethz.ch.
authors.forename: Dario
authors.initials: D
authors.lastname: Neri
mesh_terms: D000818:Animals; D000912:Antibodies, Neoplasm / Q000276:immunology / Q000627:therapeutic use; D000917:Antibody Formation; D014408:Biomarkers, Tumor / Q000276:immunology*; D002986:Clinical Trials as Topic; D016503:Drug Delivery Systems / Q000266:history / Q000379:methods; D049673:History, 20th Century; D049674:History, 21st Century; D006801:Humans; D040901:Proteomics / Q000266:history / Q000379:methods
chemical_list: D000912:Antibodies, Neoplasm; D014408:Biomarkers, Tumor
keywords: Antibody-phage libraries; Chemical proteomics; Inflammation; Mass spectrometry; Oncology