title: Integrative analysis reveals novel pathways mediating the interaction between adipose tissue and pancreatic islets in obesity in rats.
abstract: OBJECTIVE
  Comprehensive characterisation of the interrelation between the peripancreatic adipose tissue and the pancreatic islets promises novel insights into the mechanisms that regulate beta cell adaptation to obesity. Here, we sought to determine the main pathways and key molecules mediating the crosstalk between these two tissues during adaptation to obesity by the way of an integrated inter-tissue, multi-platform analysis.
  
  
  METHODS
  Wistar rats were fed a standard or cafeteria diet for 30 days. Transcriptomic variations by diet in islets and peripancreatic adipose tissue were examined through microarray analysis. The secretome from peripancreatic adipose tissue was subjected to a non-targeted metabolomic and proteomic analysis. Gene expression variations in islets were integrated with changes in peripancreatic adipose tissue gene expression and protein and metabolite secretion using an integrated inter-tissue pathway and network analysis.
  
  
  RESULTS
  The highest level of data integration, linking genes differentially expressed in both tissues with secretome variations, allowed the identification of significantly enriched canonical pathways, such as the activation of liver/retinoid X receptors, triacylglycerol degradation, and regulation of inflammatory and immune responses, and underscored interaction network hubs, such as cholesterol and the fatty acid binding protein 4, which were unpredicted through single-tissue analysis and have not been previously implicated in the peripancreatic adipose tissue crosstalk with beta cells.
  
  
  CONCLUSIONS
  The integrated analysis reported here allowed the identification of novel mechanisms and key molecules involved in peripancreatic adipose tissue interrelation with beta cells during the development of obesity; this might help the development of novel strategies to prevent type 2 diabetes.
authors.affiliation: Diabetes and Obesity Laboratory, Institut d'Investigations Biomediques August Pi I Sunyer (IDIBAPS), Carrer Rosselló 153, 08036, Barcelona, Spain.
authors.forename: Rita
authors.initials: R
authors.lastname: Malpique
authors.forename: Hugo
authors.initials: H
authors.lastname: Figueiredo
authors.forename: Yaiza
authors.initials: Y
authors.lastname: Esteban
authors.forename: Sandra A
authors.initials: SA
authors.lastname: Rebuffat
authors.forename: Felicia A
authors.initials: FA
authors.lastname: Hanzu
authors.forename: Maria
authors.initials: M
authors.lastname: Vinaixa
authors.forename: Oscar
authors.initials: O
authors.lastname: Yanes
authors.forename: Xavier
authors.initials: X
authors.lastname: Correig
authors.forename: Sílvia
authors.initials: S
authors.lastname: Barceló-Batllori
authors.forename: Rosa
authors.initials: R
authors.lastname: Gasa
authors.forename: Susana G
authors.initials: SG
authors.lastname: Kalko
authors.forename: Ramon
authors.initials: R
authors.lastname: Gomis
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D007515:Islets of Langerhans / Q000378:metabolism*; D008297:Male; D009765:Obesity / Q000378:metabolism*; D040901:Proteomics; D051381:Rats; D017208:Rats, Wistar; D014280:Triglycerides / Q000378:metabolism
chemical_list: D014280:Triglycerides