title: The scaffold protein MUPP1 regulates odorant-mediated signaling in olfactory sensory neurons.
abstract: The olfactory signal transduction cascade transforms odor information into electrical signals by a cAMP-based amplification mechanism. The mechanisms underlying the very precise temporal and spatial organization of the relevant signaling components remains poorly understood. Here, we identify, using co-immunoprecipitation experiments, a macromolecular assembly of signal transduction components in mouse olfactory neurons, organized through MUPP1. Disruption of the PDZ signaling complex, through use of an inhibitory peptide, strongly impaired odor responses and changed the activation kinetics of olfactory sensory neurons. In addition, our experiments demonstrate that termination of the response is dependent on PDZ-based scaffolding. These findings provide new insights into the functional organization, and regulation, of olfactory signal transduction.
authors.affiliation: Cell Physiology, Faculty for Biology and Biotechnology, Ruhr-Universität Bochum, Universitätsstrasse 150, 44780 Bochum, Germany.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Baumgart
authors.affiliation: Cell Physiology, Faculty for Biology and Biotechnology, Ruhr-Universität Bochum, Universitätsstrasse 150, 44780 Bochum, Germany.
authors.forename: Fabian
authors.initials: F
authors.lastname: Jansen
authors.affiliation: Cluster of Excellence NeuroCure, Charité-Universitätsmedizin Berlin, Charitéplatz 1, 10117 Berlin, Germany.
authors.forename: Willem
authors.initials: W
authors.lastname: Bintig
authors.affiliation: Cell Physiology, Faculty for Biology and Biotechnology, Ruhr-Universität Bochum, Universitätsstrasse 150, 44780 Bochum, Germany.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Kalbe
authors.affiliation: Physical Chemistry I, Ruhr-Universität Bochum, Universitätsstrasse 150, 44780 Bochum, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Herrmann
authors.affiliation: Physical Chemistry I, Ruhr-Universität Bochum, Universitätsstrasse 150, 44780 Bochum, Germany.
authors.forename: Fabian
authors.initials: F
authors.lastname: Klumpers
authors.affiliation: Inorganic Chemistry I - Bioinorganic Chemistry, Ruhr-Universität Bochum, Universitätsstrasse 150, 44780 Bochum, Germany.
authors.forename: S David
authors.initials: SD
authors.lastname: Köster
authors.affiliation: Cell Physiology, Faculty for Biology and Biotechnology, Ruhr-Universität Bochum, Universitätsstrasse 150, 44780 Bochum, Germany.
authors.forename: Paul
authors.initials: P
authors.lastname: Scholz
authors.affiliation: Cell Physiology, Faculty for Biology and Biotechnology, Ruhr-Universität Bochum, Universitätsstrasse 150, 44780 Bochum, Germany.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Rasche
authors.affiliation: Department of Molecular Medicine, Royal College of Surgeons in Ireland, Beaumont Hospital, Dublin 9, Ireland.
authors.forename: Ruth
authors.initials: R
authors.lastname: Dooley
authors.affiliation: Chair of Inorganic Chemistry I - Bioinorganic Chemistry, Ruhr-Universität Bochum, Universitätsstrasse 150, 44780 Bochum, Germany.
authors.forename: Nils
authors.initials: N
authors.lastname: Metzler-Nolte
authors.affiliation: Department of Chemosensation, Institute for Biology II, RWTH-Aachen University, Worringer Weg 1, 52074 Aachen, Germany.
authors.forename: Marc
authors.initials: M
authors.lastname: Spehr
authors.affiliation: Cell Physiology, Faculty for Biology and Biotechnology, Ruhr-Universität Bochum, Universitätsstrasse 150, 44780 Bochum, Germany.
authors.forename: Hanns
authors.initials: H
authors.lastname: Hatt
authors.affiliation: Cluster of Excellence NeuroCure, Charité-Universitätsmedizin Berlin, Charitéplatz 1, 10117 Berlin, Germany Pharmacology and Toxicology, University Hospital Jena, Drakendorfer Weg 1, 07743 Jena, Germany eva.neuhaus@med.uni-jena.de.
authors.forename: Eva M
authors.initials: EM
authors.lastname: Neuhaus
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D000242:Cyclic AMP / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D008565:Membrane Proteins; D051379:Mice; D008810:Mice, Inbred C57BL; D008840:Microfilament Proteins / Q000378:metabolism; D046912:Multiprotein Complexes / Q000378:metabolism*; D009831:Olfactory Mucosa / Q000502:physiology*; D018034:Olfactory Receptor Neurons / Q000378:metabolism; D054731:PDZ Domains / Q000235:genetics; D010446:Peptide Fragments / Q000378:metabolism; D011485:Protein Binding; D018035:Receptors, Odorant / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D002352:Carrier Proteins; D000242:Cyclic AMP; D008565:Membrane Proteins; D008840:Microfilament Proteins; D046912:Multiprotein Complexes; D010446:Peptide Fragments; D018035:Receptors, Odorant; C491750:Mpdz protein, mouse; C544141:Olfr73 protein, mouse
keywords: MUPP1; Olfactory neuron; PDZ; Scaffolding protein