title: Quercetin improves insulin resistance and hepatic lipid accumulationin vitroin a NAFLD cell model.
abstract: Non-alcoholic fatty liver disease (NAFLD) encompasses a spectrum of liver diseases in the absence of significant alcohol consumption. The aim of this study was to investigate the effect of quercetin on insulin resistance and lipid metabolic abnormalities in free fatty acid (FFA)- and insulin-induced HepG2 cell model of NAFLD, and to determine the possible underlying mechanism. Quercetin markedly improves hepatic lipid accumulation and decreases the levels of triglyceride (TG). The lipid-lowering effect of quercetin at concentrations between 0.1 and 100 μM demonstrated a dose-dependent pattern. Quercetin was found to enhance tyrosine phosphorylation in the insulin-signaling pathway and to downregulate the expression levels of the sterol regulatory element-binding protein-1c (SREBP-1c) and fatty acid synthase (FAS) in quercetin-treated groups, compared to the control group. These results demonstrated that quercetin was able to improve insulin resistance and hepatic lipid accumulation by suppressing two lipogenesis gene expression levels of SREBP-1c and FAS. As a result, quercetin has the therapeutic potential for preventing or treating NAFLD and IR-related metabolic disorders.
authors.affiliation: Department of Pharmacology, Medical College of Chifeng University, Hongshan, Chifeng, Inner Mongolia 024000;
authors.forename: Xiuli
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Pharmacology, Peking University, Health Science Center, Beijing 100191;
authors.forename: Rong
authors.initials: R
authors.lastname: Wang
authors.affiliation: Department of Pharmacology, Peking University, Health Science Center, Beijing 100191;
authors.forename: Na
authors.initials: N
authors.lastname: Zhou
authors.affiliation: Affiliated Hospital of Chifeng University, Hongshan, Chifeng, Inner Mongolia 024000, P.R. China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Pharmacology, Medical College of Chifeng University, Hongshan, Chifeng, Inner Mongolia 024000;
authors.forename: Qingyan
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Department of Pharmacology, Medical College of Chifeng University, Hongshan, Chifeng, Inner Mongolia 024000;
authors.forename: Yuqin
authors.initials: Y
authors.lastname: Bai
authors.affiliation: Department of Pharmacology, Medical College of Chifeng University, Hongshan, Chifeng, Inner Mongolia 024000;
authors.forename: Yin
authors.initials: Y
authors.lastname: Bai
authors.affiliation: Department of Pharmacology, Medical College of Chifeng University, Hongshan, Chifeng, Inner Mongolia 024000;
authors.forename: Zhijie
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Pharmacology, Medical College of Chifeng University, Hongshan, Chifeng, Inner Mongolia 024000;
authors.forename: Huiming
authors.initials: H
authors.lastname: Yang
authors.affiliation: Department of Pharmacology, Medical College of Chifeng University, Hongshan, Chifeng, Inner Mongolia 024000;
authors.forename: Jihong
authors.initials: J
authors.lastname: Zou
authors.affiliation: Department of Pharmacology, Medical College of Chifeng University, Hongshan, Chifeng, Inner Mongolia 024000;
authors.forename: Hongxia
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Pharmacology, Medical College of Chifeng University, Hongshan, Chifeng, Inner Mongolia 024000;
authors.forename: Tiewei
authors.initials: T
authors.lastname: Shi
keywords: hepatic lipid accumulation; non-alcoholic fatty liver disease; quercetin