title: Effects of intrauterine growth retardation and postnatal high-fat diet on hepatic inflammatory response in pigs.
abstract: The objective of this study was to investigate the glucose and insulin response of pigs with intrauterine growth retardation (IUGR) to a high-fat (HF) feeding regimen and to observe the underlying toll-like receptor-4 (TLR4) signalling pathway. Weaned piglets with IUGR or normal birthweight (NBW) (n = 20 each) received during the whole fattening period control diets (0% lard) or HF diets (HF, 10% lard). At about 110 kg body weight, pigs were euthanised to collect hepatic samples. Compared with NBW pigs, IUGR pigs had lower daily gain and feed intake. Growth rate of pigs was increased by HF feeding. Pigs fed HF diets had lower peak concentrations of glucose and insulin, which decreased more slowly than in pigs that received the control diets. The mRNA expression abundances of peroxisome proliferator-activated receptor coactivator-1α (PGC-1α), TLR4, myeloid differentiation factor 88 (MyD88), tumour necrosis factor-α (TNF-α), interleukin 6 (IL-6) and IL-1 receptor antagonist (IL-1Rn) were significantly affected by HF feeding, and IL-6 and IL-1Rn mRNA expressions were up-regulated in IUGR pigs compared with NBW pigs. Western blot analysis indicated that HF feeding elevated the protein expressions of TLR4 signalling pathway, as TLR4, MyD88, IκB kinase β and nuclear factor-κB, and insulin signalling-related proteins, as phosphorylated insulin receptor substrate-1 and phosphorylated protein kinase B. In summary, hepatic TLR4 signalling pathway and inflammatory response induced by HF feeding played an important role in the aggravated development of insulin resistance in pigs.
authors.affiliation: a School of Life Science and Engineering , Southwest University of Science and Technology , Mianyang , P. R. China.
authors.forename: Jingbo
authors.initials: J
authors.lastname: Liu
authors.forename: Jian
authors.initials: J
authors.lastname: He
authors.forename: Yuekui
authors.initials: Y
authors.lastname: Yang
authors.forename: Jie
authors.initials: J
authors.lastname: Yu
authors.forename: Xiangbing
authors.initials: X
authors.lastname: Mao
authors.forename: Bing
authors.initials: B
authors.lastname: Yu
authors.forename: Daiwen
authors.initials: D
authors.lastname: Chen
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000818:Animals; D001724:Birth Weight; D059305:Diet, High-Fat / Q000662:veterinary*; D004041:Dietary Fats / Q000008:administration & dosage / Q000378:metabolism*; D019587:Dietary Supplements / Q000032:analysis; D005260:Female; D005317:Fetal Growth Retardation / Q000473:pathology / Q000662:veterinary*; D005786:Gene Expression Regulation; D005947:Glucose / Q000378:metabolism*; D006520:Hepatitis, Animal / Q000473:pathology*; D007328:Insulin / Q000378:metabolism*; D008297:Male; D015398:Signal Transduction; D034421:Sus scrofa / Q000235:genetics / Q000254:growth & development / Q000276:immunology*; D051197:Toll-Like Receptor 4 / Q000235:genetics / Q000378:metabolism
chemical_list: D004041:Dietary Fats; D007328:Insulin; D051197:Toll-Like Receptor 4; D005947:Glucose; C029310:lard