title: Spectrum-effect relationships between UPLC fingerprints and bioactivities of crude secondary roots of Aconitum carmichaelii Debeaux (Fuzi) and its three processed products on mitochondrial growth coupled with canonical correlation analysis.
abstract: BACKGROUND
  The crude secondary roots of Aconitum carmichaelii Debeaux (Fuzi), together with its processed products, including Yanfuzi, Heishunpian and Paofupian, are commonly applied in clinic using for thousands of years, such as collapse, syncope, rheumatic fever, painful joints and various tumors.
  
  
  OBJECTIVE
  To explore the different effects of Fuzi and its processed products on energy metabolism, with mitochondria as the model with the aim of guiding the clinical use of Fuzi and its products. fingerprints of Fuzi, Yanfuzi, Heishunpian and Paofupian were established by Ultra-high Performance Liquid Chromatography (UPLC) and effects of Fuzi and its processed products on rat's liver׳s mitochondrial metabolism were studied by microcalorimetry. Spectrum-effect relationships between UPLC fingerprints and energy metabolism of mitochondria were investigated using canonical correlation analysis (CCA).
  
  
  RESULTS
  Because of their inherent differences in chemical compositions, the main activities of energy metabolism of mitochondria were different among Fuzi and its processed products. The potential bioactivity sequence of the tested products was Fuzi>Heishunpian>Paofupian>Yanfuzi. RESULTS of CCA showed that compounds mesaconitine, benzoylaconitine, and benzoylhypacoitine might be the principal active components.
  
  
  CONCLUSIONS
  Altogether, this work provides a general model of combination of UPLC and microcalorimetry to study the spectrum-effect relationships of Fuzi and its processed products which can offer some references for detecting principal components of traditional Chinese medicine on bioactivity to mitochondrial growth.
authors.affiliation: Pharmacy College, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China; China Military Institute of Chinese Medicine, 302 Hospital of People׳s Liberation Army, 100, the 4th Ring Road, Beijing 100039, China.
authors.forename: Quanfu
authors.initials: Q
authors.lastname: Zheng
authors.affiliation: China Military Institute of Chinese Medicine, 302 Hospital of People׳s Liberation Army, 100, the 4th Ring Road, Beijing 100039, China. Electronic address: zhaoyanling2855@126.com.
authors.forename: Yanling
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: China Military Institute of Chinese Medicine, 302 Hospital of People׳s Liberation Army, 100, the 4th Ring Road, Beijing 100039, China.
authors.forename: Jiabo
authors.initials: J
authors.lastname: Wang
authors.affiliation: Pharmacy College, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China; China Military Institute of Chinese Medicine, 302 Hospital of People׳s Liberation Army, 100, the 4th Ring Road, Beijing 100039, China.
authors.forename: Tiantian
authors.initials: T
authors.lastname: Liu
authors.affiliation: The Second Hospital Affiliated to Harbin Medical University, No 86, Baojian Road, Nangang District, Harbin, Heilongjiang Province, 150086, China. Electronic address: zhangbo_6679@126.com.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Department of Integrative Medical Center, 302 Hospital of People׳s Liberation Army, 100, the 4th Ring Road, Beijing 100039, China.
authors.forename: Man
authors.initials: M
authors.lastname: Gong
authors.affiliation: Department of Integrative Medical Center, 302 Hospital of People׳s Liberation Army, 100, the 4th Ring Road, Beijing 100039, China.
authors.forename: Jianyu
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Integrative Medical Center, 302 Hospital of People׳s Liberation Army, 100, the 4th Ring Road, Beijing 100039, China.
authors.forename: Honghong
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Pharmacy, Guang Dong Pharmacy College, 68, Nanhua District, Guangzhou 51006, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Han
authors.affiliation: China Military Institute of Chinese Medicine, 302 Hospital of People׳s Liberation Army, 100, the 4th Ring Road, Beijing 100039, China.
authors.forename: Yaming
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Integrative Medical Center, 302 Hospital of People׳s Liberation Army, 100, the 4th Ring Road, Beijing 100039, China.
authors.forename: Xueai
authors.initials: X
authors.lastname: Song
authors.affiliation: Department of Integrative Medical Center, 302 Hospital of People׳s Liberation Army, 100, the 4th Ring Road, Beijing 100039, China.
authors.forename: Yonggang
authors.initials: Y
authors.lastname: Li
authors.affiliation: China Military Institute of Chinese Medicine, 302 Hospital of People׳s Liberation Army, 100, the 4th Ring Road, Beijing 100039, China.
authors.forename: Xiaohe
authors.initials: X
authors.lastname: Xiao
mesh_terms: D000157:Aconitine / Q000031:analogs & derivatives / Q000494:pharmacology; D031841:Aconitum; D000818:Animals; D002151:Calorimetry; D002851:Chromatography, High Pressure Liquid; D008297:Male; D008516:Medicine, Chinese Traditional; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000378:metabolism; D028321:Plant Preparations / Q000494:pharmacology*; D018517:Plant Roots; D017207:Rats, Sprague-Dawley
chemical_list: D028321:Plant Preparations; D000157:Aconitine
keywords: Crude secondary roots of Aconitum carmichaelii Debeaux (Fuzi); Microcalorimetry; Mitochondria; Processed products; Spectrum-effect relationships; UPLC fingerprints