title: Cross-linked iron oxide nanoparticles for therapeutic engineering and in vivo monitoring of mesenchymal stem cells in cerebral ischemia model.
abstract: Poly(ethylene glycol)-coated cross-linked iron oxide nanoparticles (PCIONs) are developed for therapeutic engineering of mesenchymal stem cells (MSCs) and their monitoring via magnetic resonance (MR) imaging at a time. PCIONs successfully combine with plasmid DNA (pDNA) via ionic interaction. Accordingly, PCION/pDNA complexes mediate superior translocations of vascular endothelial growth factor (VEGF) pDNA into intracellular regions of MSCs under external magnetic field, which significantly elevate production of VEGF from MSCs. Genetically engineered MSCs are also clearly visualized via MR imaging after administration to rat cerebrovascular ischemia models, which enable tracking of MSCs migration from injected sites to injured ischemic area.
authors.affiliation: Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, 305-701, Republic of Korea.
authors.forename: Ji Won
authors.initials: JW
authors.lastname: Park
authors.forename: Sook Hee
authors.initials: SH
authors.lastname: Ku
authors.forename: Hyung-Ho
authors.initials: HH
authors.lastname: Moon
authors.forename: Minhyung
authors.initials: M
authors.lastname: Lee
authors.forename: Donghoon
authors.initials: D
authors.lastname: Choi
authors.forename: Jaemoon
authors.initials: J
authors.lastname: Yang
authors.forename: Yong-Min
authors.initials: YM
authors.lastname: Huh
authors.forename: Ji Hoon
authors.initials: JH
authors.lastname: Jeong
authors.forename: Tae Gwan
authors.initials: TG
authors.lastname: Park
authors.forename: Hyejung
authors.initials: H
authors.lastname: Mok
authors.forename: Sun Hwa
authors.initials: SH
authors.lastname: Kim
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D060846:Cell Engineering; D058948:Cell Tracking / Q000379:methods*; D064987:Cell- and Tissue-Based Therapy / Q000379:methods*; D004195:Disease Models, Animal; D005290:Ferric Compounds / Q000737:chemistry; D008279:Magnetic Resonance Imaging; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D053758:Nanoparticles / Q000737:chemistry*; D010957:Plasmids / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D014162:Transfection; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism
chemical_list: D005290:Ferric Compounds; D042461:Vascular Endothelial Growth Factor A; C467498:vascular endothelial growth factor A, rat; C000499:ferric oxide; D011092:Polyethylene Glycols
keywords: cell engineering; cerebral ischemia model; cross-linked iron oxide nanoparticles; magnetic resonance imaging; mesenchymal stem cells