title: Long-term disruption of growth, reproduction, and behavior after embryonic exposure of zebrafish to PAH-spiked sediment.
abstract: A natural sediment spiked with three individual polycyclic aromatic hydrocarbons (PAHs; pyrene, phenanthrene and benzo[a]pyrene) was used to expose zebrafish embryos and larvae during 4 days. The total PAH concentration was 4.4 μg g(-1) which is in the range found in sediment from contaminated areas. Quantification of metabolites in the larvae after exposure confirmed the actual contamination of the larvae and indicated an active metabolism especially for pyrene and benzo[a]pyrene. After a transfer in a clean medium, the larvae were reared to adulthood and evaluated for survival, growth, reproduction, and behavior. Measured endpoints revealed a late disruption of growth (appearing at 5 months) and a trend toward a lower reproductive ability. Adults of embryos exposed to sediment spiked with PAHs displayed lethargic and/or anxiety-like behaviors. This latter behavior was also identified in offspring at larval stage. All together, these effects could have detrimental consequences on fish performances and contribution to recruitment.
authors.affiliation: Laboratoire d'Ecotoxicologie, Ifremer, Place Gaby Coll, BP7, 17137, L'Houmeau, France.
authors.forename: Caroline
authors.initials: C
authors.lastname: Vignet
authors.forename: Marie-Hélène
authors.initials: MH
authors.lastname: Devier
authors.forename: Karyn
authors.initials: K
authors.lastname: Le Menach
authors.forename: Laura
authors.initials: L
authors.lastname: Lyphout
authors.forename: Jérémy
authors.initials: J
authors.lastname: Potier
authors.forename: Jérôme
authors.initials: J
authors.lastname: Cachot
authors.forename: Hélène
authors.initials: H
authors.lastname: Budzinski
authors.forename: Marie-Laure
authors.initials: ML
authors.lastname: Bégout
authors.forename: Xavier
authors.initials: X
authors.lastname: Cousin
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects*; D004625:Embryo, Nonmammalian / Q000187:drug effects; D005260:Female; D019015:Geologic Sediments / Q000737:chemistry*; D008297:Male; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis / Q000633:toxicity*; D012098:Reproduction / Q000187:drug effects; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000633:toxicity*; D015027:Zebrafish / Q000254:growth & development* / Q000502:physiology
chemical_list: D011084:Polycyclic Aromatic Hydrocarbons; D014874:Water Pollutants, Chemical