title: Impact of immunization with Protein F on pulmonary clearance of nontypeable Haemophilus influenzae.
abstract: Nontypeable Haemophilus influenzae (NTHi) is one of the main aetiologies of childhood bacterial infections as well as exacerbations in COPD patients. Currently, no licensed NTHi vaccine exists. In the present study, we evaluated the potential of the conserved and ubiquitous surface protein Haemophilus Protein F (PF) as a vaccine candidate. Our results show that incubation of NTHi with anti-PF antibodies significantly increased the opsonophygocytosis of human promyelocytic leukemia cell line-derived granulocytes, leading to efficient killing of the bacteria (P≤0.05). The presence of anti-PF IgG titers in healthy adults (n=60) was investigated, and we found that 26% of healthy blood donors carried antibodies with the main antigenic epitope being PF(23-48). Finally, mice immunized with PF(23-48) attained a significantly increased capacity to clear NTHi as compared to a control group immunized with a peptide derived from Moraxella catarrhalis β-lactamase (P≤0.05). Taken together, our results indicate that PF is a potential NTHi-vaccine candidate.
authors.affiliation: Medical Microbiology, Department of Laboratory Medicine Malmö, Lund University, Malmö, Sweden.
authors.forename: Farshid
authors.initials: F
authors.lastname: Jalalvand
authors.affiliation: Medical Microbiology, Department of Laboratory Medicine Malmö, Lund University, Malmö, Sweden.
authors.forename: Nils
authors.initials: N
authors.lastname: Littorin
authors.affiliation: Medical Microbiology, Department of Laboratory Medicine Malmö, Lund University, Malmö, Sweden.
authors.forename: Yu-Ching
authors.initials: YC
authors.lastname: Su
authors.affiliation: Medical Microbiology, Department of Laboratory Medicine Malmö, Lund University, Malmö, Sweden. Electronic address: kristian.riesbeck@med.lu.se.
authors.forename: Kristian
authors.initials: K
authors.lastname: Riesbeck
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D006098:Granulocytes / Q000276:immunology; D018922:HL-60 Cells; D006192:Haemophilus Infections / Q000628:therapy*; D018073:Haemophilus Vaccines / Q000627:therapeutic use*; D006193:Haemophilus influenzae; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D010587:Phagocytosis; D011817:Rabbits
chemical_list: D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D000939:Epitopes; D018073:Haemophilus Vaccines; D007074:Immunoglobulin G
keywords: ABC-transporter; Epitope mapping; Haemophilus influenzae; Immunization; Mouse; Opsonophagocytosis; Protein F; Pulmonary clearance