title: Puerarin alleviates aggravated sympathoexcitatory response induced by myocardial ischemia via regulating P2X3 receptor in rat superior cervical ganglia.
abstract: Myocardial ischemia elicits a sympathoexcitatory response characterized by increase in blood pressure and sympathetic nerve activity. Puerarin, a major active ingredient extracted from the traditional Chinese plant medicine Ge-gen, has been widely used in treatment of myocardial and cerebral ischemia. However, little is known about the mechanism. Our study was aimed to explore the effect of puerarin on sympathoexcitatory response induced by myocardial ischemic injury and possible relationship with P2X3 receptor. Our results showed that puerarin alleviated systolic blood pressure and heart rate, and decreased the up-regulated of P2X3 mRNA and protein in SCG of myocardial ischemic rats. The amplitude of ATP-activated currents of SCG neurons was much larger in myocardial ischemic group than that in control group. Puerarin reduced ATP-activated currents in myocardial ischemic group and control group, and the inhibiting effects of puerarin in myocardial ischemic group were stronger than those in control group. Puerarin also significantly inhibited ATP-activated currents in HEK293 cells transfected with P2X3 receptor. These results suggest that puerarin can depress up-sympathoexcitatory response induced by myocardial ischemia via acting on P2X3 receptor in rat SCG to protect myocardium.
authors.affiliation: Department of Physiology, Medical School of Nanchang University, Nanchang 330006, PR China.
authors.forename: Shuangmei
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Physiology, Medical School of Nanchang University, Nanchang 330006, PR China.
authors.forename: Shicheng
authors.initials: S
authors.lastname: Yu
authors.affiliation: Department of Physiology, Medical School of Nanchang University, Nanchang 330006, PR China.
authors.forename: Changshui
authors.initials: C
authors.lastname: Xu
authors.affiliation: Department of Physiology, Medical School of Nanchang University, Nanchang 330006, PR China.
authors.forename: Lichao
authors.initials: L
authors.lastname: Peng
authors.affiliation: Department of Physiology, Medical School of Nanchang University, Nanchang 330006, PR China.
authors.forename: Hong
authors.initials: H
authors.lastname: Xu
authors.affiliation: Department of Physiology, Medical School of Nanchang University, Nanchang 330006, PR China.
authors.forename: Chunping
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Physiology, Medical School of Nanchang University, Nanchang 330006, PR China.
authors.forename: Guilin
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Physiology, Medical School of Nanchang University, Nanchang 330006, PR China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Department of Physiology, Medical School of Nanchang University, Nanchang 330006, PR China.
authors.forename: Bo
authors.initials: B
authors.lastname: Fan
authors.affiliation: Department of Physiology, Medical School of Nanchang University, Nanchang 330006, PR China.
authors.forename: Qicheng
authors.initials: Q
authors.lastname: Zhu
authors.affiliation: Department of Physiology, Medical School of Nanchang University, Nanchang 330006, PR China.
authors.forename: Chaoran
authors.initials: C
authors.lastname: Zheng
authors.affiliation: Department of Physiology, Medical School of Nanchang University, Nanchang 330006, PR China.
authors.forename: Bing
authors.initials: B
authors.lastname: Wu
authors.affiliation: Department of Physiology, Medical School of Nanchang University, Nanchang 330006, PR China.
authors.forename: Miaomiao
authors.initials: M
authors.lastname: Song
authors.affiliation: Department of Physiology, Medical School of Nanchang University, Nanchang 330006, PR China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Wu
authors.affiliation: Department of Physiology, Medical School of Nanchang University, Nanchang 330006, PR China. Electronic address: liangsd@hotmail.com.
authors.forename: Shangdong
authors.initials: S
authors.lastname: Liang
mesh_terms: D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D002478:Cells, Cultured; D006801:Humans; D007529:Isoflavones / Q000494:pharmacology*; D017202:Myocardial Ischemia / Q000188:drug therapy* / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D058477:Receptors, Purinergic P2X3 / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects*; D017783:Superior Cervical Ganglion / Q000187:drug effects* / Q000378:metabolism; D013564:Sympathetic Nervous System / Q000378:metabolism; D014665:Vasodilator Agents / Q000494:pharmacology*
chemical_list: D007529:Isoflavones; D058477:Receptors, Purinergic P2X3; D014665:Vasodilator Agents; C033607:puerarin
keywords: Myocardial ischemia; P2X(3) receptor; Puerarin; Superior cervical ganglia; Sympathoexcitatory response