title: Choosing the right type of serum for different applications of human adipose tissue-derived stem cells: influence on proliferation and differentiation abilities.
abstract: OBJECTIVE
  Adipose tissue-derived stem cells (ADSCs) are thought to have great potential in regenerative medicine. A xenoprotein-free culture and handling system is desirable. To date, there is only little and contradictory information about the influence of the different types of human serum on ADSC proliferation and differentiation.
  
  
  METHODS
  First, ADSCs were cultured in media containing regular human serum (HS plus) or fetal calf serum (FCS plus) with supplementation of growth factors for three passages. During passage 4, ADSC proliferative activity and adipogenic, osteogenic and chondrogenic differentiation ability was quantified. Second, ADSCs were cultured with three different human sera (regular human serum [HS], human serum from platelet-poor plasma [SPPP] or human serum from platelet-rich plasma [SPRP]) without supplementation of platelet-derived growth factor and assessed accordingly. The growth factor content of the different types of human sera was determined by means of multiplex protein assay and enzyme-linked immunosorbent assay.
  
  
  RESULTS
  The different sera did not affect ADSC doubling time significantly (P < 0.05). Specific glycerol-3-phosphat-dehydrogenase activity was significantly lower in cultures with SPRP (P < 0.01) compared with the other media compositions. Extracellular calcium deposition was significantly higher in cells differentiated in cultures with HS or SPPP compared with those with SPRP, HS plus or FCS (P < 0.01). Glycosaminoglycan content and collagen 2 were highest in cells cultured with SPRP (P < 0.001).
  
  
  CONCLUSIONS
  Culturing ADSCs in human serum appears to be a reasonable and efficient alternative compared with FCS. With respect to the outcome of a sighted clinical application, it appears to be feasible to handle the cells in a serum suitable for the intended later use.
authors.affiliation: Clinic for Plastic and Reconstructive Surgery, Aesthetic and Preventive Medicine at Heidelberg University Hospital-ETHIANUM, Heidelberg, Germany. Electronic address: eva.koellensperger@ethianum.de.
authors.forename: Eva
authors.initials: E
authors.lastname: Koellensperger
authors.affiliation: Clinic for Plastic and Reconstructive Surgery, Aesthetic and Preventive Medicine at Heidelberg University Hospital-ETHIANUM, Heidelberg, Germany.
authors.forename: Nils
authors.initials: N
authors.lastname: Bollinger
authors.affiliation: Clinic for Plastic and Reconstructive Surgery, Aesthetic and Preventive Medicine at Heidelberg University Hospital-ETHIANUM, Heidelberg, Germany.
authors.forename: Verena
authors.initials: V
authors.lastname: Dexheimer
authors.affiliation: Department of Cardiology, University of Frankfurt, Frankfurt, Germany.
authors.forename: Felix
authors.initials: F
authors.lastname: Gramley
authors.affiliation: Clinic for Plastic and Reconstructive Surgery, Aesthetic and Preventive Medicine at Heidelberg University Hospital-ETHIANUM, Heidelberg, Germany.
authors.forename: Guenter
authors.initials: G
authors.lastname: Germann
authors.affiliation: Clinic for Plastic and Reconstructive Surgery, Aesthetic and Preventive Medicine at Heidelberg University Hospital-ETHIANUM, Heidelberg, Germany.
authors.forename: Uwe
authors.initials: U
authors.lastname: Leimer
mesh_terms: D017667:Adipocytes / Q000166:cytology; D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002417:Cattle; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D006801:Humans; D044968:Regenerative Medicine; D044967:Serum / Q000378:metabolism*; D013234:Stem Cells / Q000166:cytology*; D023822:Tissue Engineering
keywords: adipose tissue; differentiation; human serum; platelet-derived growth factor; proliferation; stem cells