title: Evaluation of Institut Georges Lopez-1 preservation solution in pig pancreas transplantation: a pilot study.
abstract: BACKGROUND
  Institut Georges Lopez-1 preservation solution (IGL-1) is an emerging extracellular-type electrolyte solution, low in viscosity, containing polyethylene glycol 35 as a colloid. Although IGL-1 has shown beneficial outcomes in kidney and liver preservation, this pilot study is the first to evaluate the efficacy of IGL-1 in pancreas transplantation (PT) compared with the University of Wisconsin solution (UW).
  
  
  METHODS
  Sixteen Landrace pigs underwent allogeneic PT with 16 hr of cold ischemia. Grafts were preserved with IGL-1 (n=8) or UW (n=8). No immunosuppression was administered. We analyzed graft function, the acute-phase response, and oxidative stress in the pancreatic graft monitoring membrane fluidity and lipid peroxidation.
  
  
  RESULTS
  All eight grafts with IGL-1, but only six with UW, were functioning. Graft failures with UW resulted from graft thrombosis. There were no differences between the two solutions in the number of normoglycemic days (IGL-1: 11.5 ± 6.2 versus UW: 8.5 ± 4.4 days, P=0.1357), nor in lipid peroxidation during 16-hr cold ischemia (P=0.672), or reperfusion (P=0.185), but IGL-1 prevented changes in membrane fluidity after reperfusion when compared with UW (P=0.026).
  
  
  CONCLUSIONS
  IGL-1 offered the same degree of safety and effectiveness as UW in our model of pig PT with 16 hr of cold ischemia.
authors.affiliation: 1 Department of Surgery, University of Zaragoza, Zaragoza, Spain. 2 Department of Pharmacology and Physiology, University of Zaragoza, Zaragoza, Spain. 3 Gastroenterology and Hepatology Department, HCU Lozano Blesa, Zaragoza, Spain. 4 Experimental Hepatic Ischemia-Reperfusion Unit, Institute of Biomedical Research, Spanish National Research Council, Barcelona, Spain. 5 Department of Biochemistry and Molecular Cell Biology, University of Zaragoza, Zaragoza, Spain. 6 Department of Human Anatomy and Histology, University of Zaragoza, Zaragoza, Spain. 7 Department of Pathology, HCU Lozano Blesa, Zaragoza, Spain. 8 Department of Surgery, ICMDM, Hospital Clinic, University of Barcelona, Barcelona, Spain. 9 Address correspondence to: Prof. Francisco A. García-Gil, M.D., Ph.D., Department of Surgery, University of Zaragoza, Domingo Miral, s/n, 50009, Zaragoza, Spain.
authors.forename: Francisco A
authors.initials: FA
authors.lastname: García-Gil
authors.forename: Lorena
authors.initials: L
authors.lastname: Fuentes-Broto
authors.forename: Carlos D
authors.initials: CD
authors.lastname: Albendea
authors.forename: María Trinidad
authors.initials: MT
authors.lastname: Serrano
authors.forename: Joan
authors.initials: J
authors.lastname: Roselló-Catafau
authors.forename: Fermín
authors.initials: F
authors.lastname: Lampreave
authors.forename: Laura
authors.initials: L
authors.lastname: López-Pingarrón
authors.forename: Jorge
authors.initials: J
authors.lastname: Escartín
authors.forename: Joaquín
authors.initials: J
authors.lastname: Soria
authors.forename: Joaquín J
authors.initials: JJ
authors.lastname: Garcia
authors.forename: Laureano
authors.initials: L
authors.lastname: Fernández-Cruz
mesh_terms: D000241:Adenosine / Q000737:chemistry; D000493:Allopurinol / Q000737:chemistry; D000818:Animals; D003102:Colloids / Q000737:chemistry; D004573:Electrolytes; D005260:Female; D005978:Glutathione / Q000737:chemistry; D007165:Immunosuppression Therapy; D007328:Insulin / Q000737:chemistry; D007511:Ischemia; D007668:Kidney / Q000473:pathology; D015227:Lipid Peroxidation; D008099:Liver / Q000473:pathology; D009926:Organ Preservation / Q000379:methods*; D019188:Organ Preservation Solutions / Q000737:chemistry*; D018384:Oxidative Stress; D010179:Pancreas / Q000473:pathology*; D016035:Pancreas Transplantation / Q000379:methods*; D010865:Pilot Projects; D011092:Polyethylene Glycols / Q000737:chemistry; D011887:Raffinose / Q000737:chemistry; D013552:Swine; D013997:Time Factors; D014783:Viscosity
chemical_list: D003102:Colloids; D004573:Electrolytes; C504757:IGL-1 solution; D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D011092:Polyethylene Glycols; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose