title: Label-free cell-based dynamic mass redistribution assays.
abstract: Label-free cell-based assays offer a powerful approach to drug discovery and compound profiling for endogenously expressed receptors in a variety of cell types, including primary and stem cells. Dynamic mass redistribution (DMR) responses in whole cells following receptor stimulation provide phenotypic activity profiles that are readily amenable to evaluation of compound pharmacology. Protocols are provided in this unit to obtain DMR response profiles in adherent and suspension cells, and then to use known tool compounds to delineate the biology of the underlying signaling pathways from the information-rich kinetic traces that are recorded.
authors.affiliation: Corning Life Sciences, Kennebunk, Maine.
authors.forename: Hannah J
authors.initials: HJ
authors.lastname: Gitschier
authors.affiliation: Corning Life Sciences, Kennebunk, Maine.
authors.forename: Audrey B
authors.initials: AB
authors.lastname: Bergeron
authors.affiliation: Corning Life Sciences, Kennebunk, Maine.
authors.forename: David H
authors.initials: DH
authors.lastname: Randle
mesh_terms: D000818:Animals; D001681:Biological Assay / Q000379:methods*; D016466:CHO Cells; D045744:Cell Line, Tumor; D003412:Cricetulus; D003627:Data Interpretation, Statistical; D055808:Drug Discovery / Q000379:methods*; D006801:Humans; D061251:Primary Cell Culture; D011955:Receptors, Drug / Q000819:agonists / Q000037:antagonists & inhibitors / Q000187:drug effects; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D011955:Receptors, Drug
keywords: cell surface receptors; drug discovery; dynamic mass redistribution; label-free; optical biosensor; phenotypic assays; primary cells; signal transduction pathways