title: Prognostic relevance of HSP70 antigen and antibody measurement in patients with acute myeloid leukemia of intermediate and unfavorable cytogenetic risk.
abstract: BACKGROUND
  Heat shock proteins (HSPs) are overexpressed in many types of cancers and are implicated in tumor cell proliferation, differentiation, invasion, metastasis, death, and recognition by the immune system. It has been postulated that the HSP70 protein can be used as a prognostic indicator of overall patient survival in many types of cancer including leukemia.
  
  
  OBJECTIVE
  The aim of the study was to evaluate the concentrations of anti‑HSP70 antibody and its antigen in the peripheral blood of patients with acute myeloid leukemia (AML), as well as to assess the usefulness of this measurement.
  
  
  METHODS
  The study included 80 patients with AML of intermediate and high cytogenetic risk, scheduled for allogenic stem cell transplantation after initial intensive chemotherapy. Plasma concentrations of anti‑HSP70 antibodies and HSP70 antigen were measured by enzyme‑linked immunosorbent assay. The antigen was additionally measured by Western blot analysis. The control group consisted of healthy subjects.
  
  
  RESULTS
  Patients with AML had significantly higher anti‑HSP70 antibody concentrations compared with the control group. The concentration of HSP70 antigen as well as anti‑HSP70 antibody showed no associations with the type of response after induction chemotherapy. However, patients with higher antigen levels and lower anti‑HSP70 antibody levels had significantly shorter overall survival.
  
  
  CONCLUSIONS
  The study suggests that anti‑HSP70 antibodies and HSP70 antigen may be valuable indicators of a poor prognosis in patients with AML.
authors.forename: Jarosław
authors.initials: J
authors.lastname: Piszcz
authors.forename: Łukasz
authors.initials: Ł
authors.lastname: Bolkun
authors.forename: Edyta
authors.initials: E
authors.lastname: Cichocka
authors.forename: Marzenna
authors.initials: M
authors.lastname: Galar
authors.forename: Adam
authors.initials: A
authors.lastname: Hołownia
authors.forename: Janusz
authors.initials: J
authors.lastname: Kłoczko
mesh_terms: D000906:Antibodies / Q000097:blood*; D000941:Antigens / Q000097:blood*; D014408:Biomarkers, Tumor / Q000097:blood*; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000276:immunology*; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000276:immunology* / Q000401:mortality* / Q000628:therapy; D008297:Male; D011379:Prognosis; D015996:Survival Rate
chemical_list: D000906:Antibodies; D000941:Antigens; D014408:Biomarkers, Tumor; D018840:HSP70 Heat-Shock Proteins