title: [Universal diagnostic oligonucleotide microarray for subtyping of human and animal influenza A viruses].
abstract: The diagnostic oligonucleotide microarray for subtyping of human and animal influenza A viruses (IAVs) was developed. We proposed a simple method of the fluorescent labeling of genomic segments of all known IAVs subtypes, the composition of the hybridization buffer, as well as the software of the data processing. 48 IAVs strains of different subtypes were analyzed using our microarray. All of them were identified, while 45 of 48 strains were unambiguously subtyped.
authors.forename: A V
authors.initials: AV
authors.lastname: Vasin
authors.forename: N T
authors.initials: NT
authors.lastname: Sandybaev
authors.forename: M A
authors.initials: MA
authors.lastname: Plotnikova
authors.forename: S A
authors.initials: SA
authors.lastname: Klotchenko
authors.forename: O V
authors.initials: OV
authors.lastname: Cherviakova
authors.forename: V M
authors.initials: VM
authors.lastname: Strochkov
authors.forename: É T
authors.initials: ÉT
authors.lastname: Taĭlakova
authors.forename: O A
authors.initials: OA
authors.lastname: Temkina
authors.forename: A V
authors.initials: AV
authors.lastname: Brodskaia
authors.forename: Ia A
authors.initials: IaA
authors.lastname: Zabrodskaia
authors.forename: K P
authors.initials: KP
authors.lastname: Nikulenkov
authors.forename: V V
authors.initials: VV
authors.lastname: Egorov
authors.forename: Zh K
authors.initials: ZhK
authors.lastname: Koshemetov
authors.forename: A R
authors.initials: AR
authors.lastname: Sancyzbaĭ
authors.forename: O I
authors.initials: OI
authors.lastname: Kiselev
mesh_terms: D000818:Animals; D016679:Genome, Viral; D006801:Humans; D009980:Influenza A virus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D007251:Influenza, Human / Q000175:diagnosis / Q000821:virology; D056656:Lab-On-A-Chip Devices; D058889:Molecular Typing / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation*; D009976:Orthomyxoviridae Infections / Q000175:diagnosis / Q000821:virology*; D012367:RNA, Viral / Q000145:classification* / Q000235:genetics; D012984:Software
chemical_list: D012367:RNA, Viral