title: Effect of Pseudomonas sp. HF-1 inoculum on construction of a bioaugmented system for tobacco wastewater treatment: analysis from quorum sensing.
abstract: To better construct a bioaugmented system for tobacco wastewater treatment, activated sludge was inoculated with different concentrations of the nicotine-degrading bacterium Pseudomonas sp. HF-1. The results showed that inoculum concentrations of 0.55 ± 0.01 and 1.10 ± 0.03 mg/g (dry weight of strain HF-1/dry weight of activated sludge) were best to ensure strain HF-1 survival and successful bioaugmentation. The release pattern of autoinducer (AI) for quorum sensing in the bioaugmented system was also investigated. During the period of HF-1 inoculation, compared with failed bioaugmented systems, AI-2 was significantly increased in the successful systems, suggesting that AI-2-mediated bacterial communication played an important role in the colonization of HF-1. When inoculation of strain HF-1 was stopped, the amount of AI-2 decreased and leveled out in all systems. Notably, there was a greater than threefold increase of short-chain AHLs in failed bioaugmented systems, but no increase in successful ones, implying that the fluctuation of short-chain AHLs could be an indicator of the failure of bioaugmentation. Thus, AI-2-mediated quorum sensing could be implemented to facilitate HF-1 colonization.
authors.forename: Mei-Zhen
authors.initials: MZ
authors.lastname: Wang
authors.forename: Hong-Zhen
authors.initials: HZ
authors.lastname: He
authors.forename: Xin
authors.initials: X
authors.lastname: Zheng
authors.forename: Hua-Jun
authors.initials: HJ
authors.lastname: Feng
authors.forename: Zhen-Mei
authors.initials: ZM
authors.lastname: Lv
authors.forename: Dong-Sheng
authors.initials: DS
authors.lastname: Shen
mesh_terms: D001673:Biodegradation, Environmental; D007220:Industrial Waste / Q000032:analysis*; D011549:Pseudomonas / Q000378:metabolism*; D053038:Quorum Sensing; D014026:Nicotiana / Q000737:chemistry*; D062065:Wastewater / Q000737:chemistry*
chemical_list: D007220:Industrial Waste; D062065:Wastewater