title: Lymphoid metastasis of rat My2/De leukemia.
abstract: By grafting spontaneous leukemia tumor cells, the myeloid My2/De leukemia rat model was established. Death was caused by impaired functions of heavily infiltrated organs. In vitro culturing of tumor cells, blood and bone marrow counts and cytochemic reactions indicated the leukemic the origin resembling human myeoloblastic leukemia. Metastatic spread was followed after i.v. and i.p. injection, and by implantation of leukemia cells under the renal capsule of rats. Primary tumor and metastasis formation was visualized by (18)FDG or (11)C-methionine administration and MiniPET. The accumulation of radiotracers was measured in different organs and expressed as Differential Absorption Ratios (DARs). Subrenal implantation of My2/De cells resulted in their appearance in other abdominal organs and in parathymic lymph nodes. The release of tumor cells from the primary kidney to the peritoneum was mimicked by the i.p. administration of ink particles. Ink particles deposited in the abdominal organs and in the thoracal lymph nodes, preferentially in parathymic lymph nodes, confirming the notion of lymphatic spread of metastasis.
authors.affiliation: Department of Nuclear Medicine, University of Debrecen, Hungary.
authors.forename: G
authors.initials: G
authors.lastname: Trencsenyi
authors.affiliation: Department of Microbial Biotechnology and Cell Biology, University of Debrecen, Hungary.
authors.forename: G
authors.initials: G
authors.lastname: Nagy
authors.affiliation: Department of Microbial Biotechnology and Cell Biology, University of Debrecen, Hungary.
authors.forename: B
authors.initials: B
authors.lastname: Kahlik
authors.affiliation: Department of Nuclear Medicine, University of Debrecen, Hungary.
authors.forename: E
authors.initials: E
authors.lastname: Nemeth
authors.affiliation: Institute of Preventive Medicine, University of Debrecen, Hungary.
authors.forename: P
authors.initials: P
authors.lastname: Kertai
authors.affiliation: Department of Internal Medicine, University of Debrecen, Hungary.
authors.forename: A
authors.initials: A
authors.lastname: Kiss
authors.affiliation: Department of Microbial Biotechnology and Cell Biology, University of Debrecen, Hungary. Electronic address: bgaspar@delfin.klte.hu.
authors.forename: G
authors.initials: G
authors.lastname: Banfalvi
mesh_terms: D000818:Animals; D019788:Fluorodeoxyglucose F18; D007951:Leukemia, Myeloid / Q000473:pathology*; D008207:Lymphatic Metastasis; D008297:Male; D009195:Peroxidase / Q000032:analysis; D049268:Positron-Emission Tomography; D051381:Rats; D020318:Rats, Long-Evans
chemical_list: D019788:Fluorodeoxyglucose F18; D009195:Peroxidase
keywords: Cell culture; Cytochemical reactions; MiniPET; Monocytic myeloid leukemia; Parathymic lymph nodes; Spontaneous leukemia