title: A versatile method for encapsulating large-sized DNA into small-sized bioreducible nanocapsules.
abstract: Encapsulation of negatively charged plasmid DNA into a small-sized nanocapsule without using any condensing agent is very challenging up to now. Here we report a versatile method for encapsulating large-sized plasmid DNAs into small-sized bioreducible nanocapsules in which shearing force and surfactant can fold large-sized plasmid DNAs into small-sized emulsion droplets containing bioreducible branched polymers. Subsequently, temperature triggers the bioreducible branched polymers to aggregate and cross-link at the water/oil interface of the emulsion nanodroplet, forming a bioreducible shell around the nanodroplet. Thus, a small-sized nanocapsule (∼110 nm) containing large-sized plasmid DNA (∼1900 nm long) forms by removal of the surfactant.
authors.affiliation: CAS Key Lab of Soft Matter Chemistry, Department of Polymer Science and Engineering, University of Science and Technology of China , Hefei 230026, Anhui, People's Republic of China.
authors.forename: Long-Hai
authors.initials: LH
authors.lastname: Wang
authors.forename: Sheng-Gang
authors.initials: SG
authors.lastname: Ding
authors.forename: Jun-Jie
authors.initials: JJ
authors.lastname: Yan
authors.forename: Ye-Zi
authors.initials: YZ
authors.lastname: You
mesh_terms: D004247:DNA / Q000737:chemistry / Q000378:metabolism; D004655:Emulsions / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D053769:Nanocapsules / Q000737:chemistry*; D009821:Oils / Q000737:chemistry; D010316:Particle Size; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism*; D011108:Polymers / Q000737:chemistry; D013696:Temperature; D014162:Transfection; D014867:Water / Q000737:chemistry
chemical_list: D004655:Emulsions; D053769:Nanocapsules; D009821:Oils; D011108:Polymers; D014867:Water; D004247:DNA