title: Flow cytometric analysis of the basophil cell activating impact of potential drug delivery nanoparticle-candidate.
abstract: BACKGROUND
  Carbon nanotubes – as artificial nano-size ranged materials –have increasing role in the modern biomedical, diagnostic and therapeutic applications.There is a promising option for their use as more potential drug carriers. Despite the favourable properties, their impact (accumulation, elimination, etc.) on biological systems is largely unknown. The main limiting factor of medical use of nanomaterials in most cases is the potential hypersensitive side effect. It can develop in different route, but the activation of basophil granulocytes may play a central role in this process.
  
  
  OBJECTIVE
  Our aim was to test the direct activation ability of different, surface modified nanotubes on basophil granulocytes in vitro. In parallel we tested the effectiveness of BasoTest planned to use for this study.
  
  
  METHODS
  Using the blood samples of allergic and healthy volunteers we examined the basophil degranulation in the presence of nanotubes and the expression level changes of cell-surface CD63 on FACS Calibur instrument. Our results were compared to positive(fMLP, Mite, Grass) and negative control samples.
  
  
  RESULTS
  The test we have chosen proved to be sufficiently sensitive and specific for further study. Significant basophil activation was observed in the presence of carbon nanotubes in healthy persons and allergic patients, as well. The activating effect of nanotubes was more prevailed in allergic population.
  
  
  CONCLUSIONS
  Our experiments have proven the fact that nanotubes may play a role in the development of hypersensitive allergic reactions through their basophil granulocyte activator effect.
authors.affiliation: University of Miskolc Department of Nanobiotechnology and Regenerative Medicine, Faculty of Health Miskolc Hungary.
authors.forename: Eva
authors.initials: E
authors.lastname: Kiss-Tóth Dojcsák
authors.affiliation: University of Miskolc Department of Nanobiotechnology and Regenerative Medicine, Faculty of Health Miskolc Hungary.
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Ferenczi
authors.affiliation: University of Miskolc Department of Nanobiotechnology and Regenerative Medicine, Faculty of Health Miskolc Hungary.
authors.forename: Adrienn
authors.initials: A
authors.lastname: Szalai Juhász
authors.affiliation: University of Miskolc Department of Preventive Health Sciences, Faculty of Health Miskolc Hungary.
authors.forename: Emőke
authors.initials: E
authors.lastname: Kiss-Tóth
authors.affiliation: University of Miskolc Department of Nanobiotechnology and Regenerative Medicine, Faculty of Health Miskolc Hungary Šafárik University Department of Pathological Physiology, Medical Faculty Košice Slovakia.
authors.forename: Olivér
authors.initials: O
authors.lastname: Rácz
authors.affiliation: University of Miskolc Department of Nanobiotechnology and Regenerative Medicine, Faculty of Health Miskolc Hungary.
authors.forename: Bertalan
authors.initials: B
authors.lastname: Fodor
mesh_terms: D001491:Basophils / Q000502:physiology*; D016503:Drug Delivery Systems; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D006967:Hypersensitivity / Q000209:etiology*; D037742:Nanotubes, Carbon / Q000009:adverse effects*
chemical_list: D037742:Nanotubes, Carbon
keywords: allergy; basophil granulocyte; carbon nanotubes