title: Detection of Schistosoma mansoni antibodies in a low-endemicity area using indirect immunofluorescence and circumoval precipitin test.
abstract: Parasitological diagnostic methods for schistosomiasis lack sensitivity, especially in regions of low endemicity. The objective of this study was to determine the prevalence of Schistosoma mansoni infections by antibody detection using the indirect immunofluorescence assay (IFA-IgM) and circumoval precipitin test (COPT). Serum samples of 572 individuals were randomly selected. The IFA-IgM and COPT were used to detect anti-S. mansoni antibodies. Of the patients studied, 15.9% (N = 91) were IFA-IgM positive and 5.1% (N = 29) had COPT reactions (P < 0.001 by McNemar's test). Immunodiagnostic techniques showed higher infection prevalence than had been previously estimated. This study suggests that combined use of these diagnostic tools could be useful for the diagnosis of schistosomiasis in epidemiological studies in areas of low endemicity.
authors.affiliation: Department of Infectious and Parasitic Diseases, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil; Department of Enteroparasites at the Parasitology and Mycology, Instituto Adolfo Lutz, São Paulo, Brazil; Laboratory of Tropical Gastroenterology and Hepatology, Department of Gastroenterology, Faculdade de Medicina da Universiddae de São Paulo, Brazil; Laboratory of Clinical Parasitology of the Central Laboratory, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil; Laboratory of Tropical Gastroenterology and Hepatology, Department of Gastroenterology, Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, São Paulo, Brazil cristinasanto@usp.br.
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Carvalho do Espírito-Santo
authors.affiliation: Department of Infectious and Parasitic Diseases, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil; Department of Enteroparasites at the Parasitology and Mycology, Instituto Adolfo Lutz, São Paulo, Brazil; Laboratory of Tropical Gastroenterology and Hepatology, Department of Gastroenterology, Faculdade de Medicina da Universiddae de São Paulo, Brazil; Laboratory of Clinical Parasitology of the Central Laboratory, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil; Laboratory of Tropical Gastroenterology and Hepatology, Department of Gastroenterology, Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, São Paulo, Brazil.
authors.forename: Pedro Luiz
authors.initials: PL
authors.lastname: Pinto
authors.affiliation: Department of Infectious and Parasitic Diseases, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil; Department of Enteroparasites at the Parasitology and Mycology, Instituto Adolfo Lutz, São Paulo, Brazil; Laboratory of Tropical Gastroenterology and Hepatology, Department of Gastroenterology, Faculdade de Medicina da Universiddae de São Paulo, Brazil; Laboratory of Clinical Parasitology of the Central Laboratory, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil; Laboratory of Tropical Gastroenterology and Hepatology, Department of Gastroenterology, Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, São Paulo, Brazil.
authors.forename: Cybele
authors.initials: C
authors.lastname: Gargioni
authors.affiliation: Department of Infectious and Parasitic Diseases, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil; Department of Enteroparasites at the Parasitology and Mycology, Instituto Adolfo Lutz, São Paulo, Brazil; Laboratory of Tropical Gastroenterology and Hepatology, Department of Gastroenterology, Faculdade de Medicina da Universiddae de São Paulo, Brazil; Laboratory of Clinical Parasitology of the Central Laboratory, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil; Laboratory of Tropical Gastroenterology and Hepatology, Department of Gastroenterology, Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, São Paulo, Brazil.
authors.forename: Monica Viviana
authors.initials: MV
authors.lastname: Alvarado-Mora
authors.affiliation: Department of Infectious and Parasitic Diseases, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil; Department of Enteroparasites at the Parasitology and Mycology, Instituto Adolfo Lutz, São Paulo, Brazil; Laboratory of Tropical Gastroenterology and Hepatology, Department of Gastroenterology, Faculdade de Medicina da Universiddae de São Paulo, Brazil; Laboratory of Clinical Parasitology of the Central Laboratory, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil; Laboratory of Tropical Gastroenterology and Hepatology, Department of Gastroenterology, Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, São Paulo, Brazil.
authors.forename: Vera Lúcia
authors.initials: VL
authors.lastname: Pagliusi Castilho
authors.affiliation: Department of Infectious and Parasitic Diseases, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil; Department of Enteroparasites at the Parasitology and Mycology, Instituto Adolfo Lutz, São Paulo, Brazil; Laboratory of Tropical Gastroenterology and Hepatology, Department of Gastroenterology, Faculdade de Medicina da Universiddae de São Paulo, Brazil; Laboratory of Clinical Parasitology of the Central Laboratory, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil; Laboratory of Tropical Gastroenterology and Hepatology, Department of Gastroenterology, Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, São Paulo, Brazil.
authors.forename: João Ranato Rebello
authors.initials: JR
authors.lastname: Pinho
authors.affiliation: Department of Infectious and Parasitic Diseases, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil; Department of Enteroparasites at the Parasitology and Mycology, Instituto Adolfo Lutz, São Paulo, Brazil; Laboratory of Tropical Gastroenterology and Hepatology, Department of Gastroenterology, Faculdade de Medicina da Universiddae de São Paulo, Brazil; Laboratory of Clinical Parasitology of the Central Laboratory, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil; Laboratory of Tropical Gastroenterology and Hepatology, Department of Gastroenterology, Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, São Paulo, Brazil.
authors.forename: Expedito José
authors.initials: EJ
authors.lastname: de Albuquerque Luna
authors.affiliation: Department of Infectious and Parasitic Diseases, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil; Department of Enteroparasites at the Parasitology and Mycology, Instituto Adolfo Lutz, São Paulo, Brazil; Laboratory of Tropical Gastroenterology and Hepatology, Department of Gastroenterology, Faculdade de Medicina da Universiddae de São Paulo, Brazil; Laboratory of Clinical Parasitology of the Central Laboratory, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil; Laboratory of Tropical Gastroenterology and Hepatology, Department of Gastroenterology, Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, São Paulo, Brazil.
authors.forename: Ronaldo Cesar
authors.initials: RC
authors.lastname: Borges Gryschek
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D001938:Brazil; D002648:Child; D002675:Child, Preschool; D003430:Cross-Sectional Studies; D005243:Feces / Q000469:parasitology; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods*; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood; D007223:Infant; D008297:Male; D008875:Middle Aged; D011233:Precipitin Tests / Q000379:methods*; D012550:Schistosoma mansoni / Q000276:immunology* / Q000302:isolation & purification; D012555:Schistosomiasis mansoni / Q000175:diagnosis* / Q000469:parasitology; D016036:Seroepidemiologic Studies; D055815:Young Adult
chemical_list: D000909:Antibodies, Helminth; D007075:Immunoglobulin M