title: The transcriptomic response to copper exposure by the gill tissue of Japanese scallops (Mizuhopecten yessoensis) using deep-sequencing technology.
abstract: The bivalve Mizuhopecten yessoensis has been greatly impacted by marine pollutants in northern China. To elucidate the toxicological mechanism of copper exposure on the immune system, we investigated differentially expressed genes (DEGs) and transcript abundance in M. yessoensis gill tissue using the deep-sequencing platform Illumina HiSeq™ 2000. In total, 1312 and 2237 genes were identified as significantly up- or down-regulated, respectively. In addition, significant enrichment analysis identified 9 GO terms and 38 pathways involved in the response to copper exposure. The analysis of immune-related transcripts revealed a complex repertoire of innate recognition receptors, including toll-like receptors, NOD-like receptors and RIG-like receptors. Downstream pathway effectors, such as apoptotic, lysosomal and C-type lectin transcripts, were also analyzed. These results will provide a resource for subsequent gene expression studies regarding heavy metal exposure and the identification of copper-sensitive biomarkers to monitor the aquaculture of M. yessoensis.
authors.affiliation: College of Fisheries, Henan Normal University, Xinxiang 453007, PR China.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Meng
authors.affiliation: College of Fisheries, Henan Normal University, Xinxiang 453007, PR China.
authors.forename: Xue
authors.initials: X
authors.lastname: Tian
authors.affiliation: R&D Center of Marine, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, PR China. Electronic address: liumei@ms.qdio.ac.cn.
authors.forename: Mei
authors.initials: M
authors.lastname: Liu
authors.affiliation: College of Fisheries, Henan Normal University, Xinxiang 453007, PR China.
authors.forename: Guoxing
authors.initials: G
authors.lastname: Nie
authors.affiliation: R&D Center of Marine, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, PR China.
authors.forename: Keyong
authors.initials: K
authors.lastname: Jiang
authors.affiliation: R&D Center of Marine, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, PR China.
authors.forename: Baojie
authors.initials: B
authors.lastname: Wang
authors.affiliation: R&D Center of Marine, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, PR China. Electronic address: wanglei@ms.qdio.ac.cn.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
mesh_terms: D000818:Animals; D003300:Copper / Q000633:toxicity*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D005880:Gills / Q000187:drug effects / Q000378:metabolism; D059014:High-Throughput Nucleotide Sequencing; D008297:Male; D008969:Molecular Sequence Data; D049897:Pectinidae / Q000187:drug effects* / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D017423:Sequence Analysis, RNA; D059467:Transcriptome
chemical_list: D003300:Copper
keywords: Copper; Deep-sequencing; Gill; Mizuhopecten yessoensis