title: Plaque fluoride concentrations associated to the use of conventional and low-fluoride dentifrices.
abstract: OBJECTIVE
  This double-blind, crossover study evaluated whole plaque fluoride concentration (F), as well as whole plaque calcium concentrations (Ca) after brushing with a placebo (PD - fluoride free), low-fluoride (LFD, 513 microg F/g) and conventional (CD, 1,072 microg F/g) dentifrices.
  
  
  METHODS
  Children (n=20) were randomly assigned to brush twice daily with one of the dentifrices, during 7 days. On the 7th day, samples were collected at 1 and 12 hours after brushing. F and Ca were analyzed with an ion-selective electrode and with the Arsenazo III method, respectively. Data were analyzed by ANOVA, Tukey's test and by Pearson correlation coefficient (P< 0.05).
  
  
  RESULTS
  The use of the fluoridated dentifrices significantly increased plaque [F]s 1 hour after brushing when compared to PD, returning to baseline levels 12 hours after. Positive and significant correlations were found between plaque [F] and (Ca) under most of the conditions evaluated. The mean increase in plaque [F] observed 1 hour after brushing with the CD were only about 47% higher than those obtained for the LFD. The use of a LFD promotes proportionally higher increases in plaque [F] when compared to a CD. Plaque F concentrations were also shown to be dependent on plaque Ca concentrations.
authors.forename: Juliano Pelim
authors.initials: JP
authors.lastname: Pessan
authors.forename: Michele Mauricio
authors.initials: MM
authors.lastname: Manarelli
authors.forename: Karina Yuri
authors.initials: KY
authors.lastname: Kondo
authors.forename: Flávia Moraes
authors.initials: FM
authors.lastname: de Italiani
authors.forename: Cláudia Ayumi Nakai
authors.initials: CA
authors.lastname: Kobayashi
authors.forename: Gary Milton
authors.initials: GM
authors.lastname: Whitford
authors.forename: Marília Afonso Rabelo
authors.initials: MA
authors.lastname: Buzalaf
mesh_terms: D001150:Arsenazo III; D002118:Calcium / Q000032:analysis; D002327:Cariostatic Agents / Q000008:administration & dosage / Q000032:analysis* / Q000627:therapeutic use; D002648:Child; D004396:Coloring Agents; D018592:Cross-Over Studies; D003773:Dental Plaque / Q000737:chemistry*; D003802:Dentifrices / Q000008:administration & dosage / Q000627:therapeutic use*; D004305:Dose-Response Relationship, Drug; D004311:Double-Blind Method; D005459:Fluorides / Q000008:administration & dosage / Q000032:analysis* / Q000627:therapeutic use; D006801:Humans; D017736:Ion-Selective Electrodes; D010919:Placebos; D013997:Time Factors; D014099:Toothbrushing / Q000379:methods
chemical_list: D002327:Cariostatic Agents; D004396:Coloring Agents; D003802:Dentifrices; D010919:Placebos; D001150:Arsenazo III; D005459:Fluorides; D002118:Calcium