title: Elucidation of the structure of the oligosaccharide from wild type Moraxella bovis Epp63 lipooligosaccharide.
abstract: Moraxella bovis is a Gram-negative microorganism that causes Infectious Bovine Keratoconjunctivitis (IBK), colloquially known as 'Pink eye' in cattle worldwide. Lipopolysaccharides/lipooligosaccharides are the predominant glycans on the surface of Gram-negative microorganisms. Structural elucidation of the oligosaccharide structure of the rough phenotype of Moraxella bovis strain Epp63 was determined using GC-MS, methylation analysis, and NMR spectroscopy. The oligosaccharide is a branched structure that comprises 10 sugars in addition to KDO. The unusual features of this oligosaccharide include the fact that the oligosaccharide is devoid of heptose. The KDO residue is directly attached to a (→4,6)-branched glucose and additionally contains a terminal open chain acetal-linked N-acetylgalactosamine, (1S)-GalaNAc residue →4,6-linked to a sub-terminal galactose residue.
authors.affiliation: Dipartimento di Scienze Chimiche, Università di Napoli Federico II, Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126 Napoli, Italy. Electronic address: decastro@unina.it.
authors.forename: Cristina
authors.initials: C
authors.lastname: De Castro
authors.affiliation: Institute for Glycomics and School of Medical Science, Griffith University Gold Coast Campus, Queensland 4222, Australia. Electronic address: d.grice@griffith.edu.au.
authors.forename: I Darren
authors.initials: ID
authors.lastname: Grice
authors.affiliation: Institute for Glycomics and School of Medical Science, Griffith University Gold Coast Campus, Queensland 4222, Australia. Electronic address: t.daal@griffith.edu.au.
authors.forename: Terese-Marie
authors.initials: TM
authors.lastname: Daal
authors.affiliation: Institute for Glycomics and School of Medical Science, Griffith University Gold Coast Campus, Queensland 4222, Australia. Electronic address: i.peak@griffith.edu.au.
authors.forename: Ian R
authors.initials: IR
authors.lastname: Peak
authors.affiliation: Dipartimento di Scienze Chimiche, Università di Napoli Federico II, Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126 Napoli, Italy. Electronic address: molinaro@unina.it.
authors.forename: Antonio
authors.initials: A
authors.lastname: Molinaro
authors.affiliation: Institute for Glycomics and School of Medical Science, Griffith University Gold Coast Campus, Queensland 4222, Australia. Electronic address: jennifer.wilson@griffith.edu.au.
authors.forename: Jennifer C
authors.initials: JC
authors.lastname: Wilson
mesh_terms: D000116:Acetylgalactosamine / Q000032:analysis; D002240:Carbohydrate Sequence; D005690:Galactose / Q000032:analysis; D005947:Glucose / Q000032:analysis; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D016955:Moraxella bovis / Q000737:chemistry*; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial; C023023:lipid-linked oligosaccharides; D005947:Glucose; D000116:Acetylgalactosamine; D005690:Galactose
keywords: Core oligosaccharide; Lipooligosaccharide; Moraxella bovis surface antigen; Structural elucidation