title: Adipose tissue dendritic cells enhances inflammation by prompting the generation of Th17 cells.
abstract: BACKGROUND
  Obesity has become a global challenge for public health. It has been reported that obesity is associated with chronic inflammation. However, the mechanism for the chronic inflammation contributes to obesity remains elusive.
  
  
  RESULTS
  In our study, we found a novel CD11c+ dendritic cell subset existed in murine adipose tissues which was immature phenotype. Moreover, as compared to the lean controls, the number of CD11c+ DCs and CD4+IL-17+T cells were higher in adipose tissue of high fat diet (HFD) mice. Adipose tissues derived dendritic cells (ATDCs) displayed lower levels of CD40, CD80, CD86, MHCI and MHCII expression than splenic DCs (SPDCs). However, ATDCs showed higher levels of IL-6, TGF-β and IL-23 secretion. Moreover, our in vitro experiments demonstrated that ATDCs were capable of promoting Th17 cell generation.
  
  
  CONCLUSIONS
  Our results indicate the existence of CD11c+ DCs in adipose tissues, which displays an immature phenotype but possessing pro-inflammatory function.
authors.affiliation: Department of Laboratory Medicine, The Affiliated People's Hospital, Jiangsu University, Zhenjiang, China.
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Laboratory Medicine, The Affiliated People's Hospital, Jiangsu University, Zhenjiang, China; Institute of Laboratory Medicine, Jiangsu University School of Medical Science and Laboratory Medicine, Zhenjiang, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Tian
authors.affiliation: Department of Laboratory Medicine, The Affiliated People's Hospital, Jiangsu University, Zhenjiang, China.
authors.forename: Xinyu
authors.initials: X
authors.lastname: Tian
authors.affiliation: Department of Laboratory Medicine, The Affiliated People's Hospital, Jiangsu University, Zhenjiang, China.
authors.forename: Xinyi
authors.initials: X
authors.lastname: Tang
authors.affiliation: Department of Laboratory Medicine, The Affiliated People's Hospital, Jiangsu University, Zhenjiang, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Rui
authors.affiliation: Department of Laboratory Medicine, The Affiliated People's Hospital, Jiangsu University, Zhenjiang, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Tong
authors.affiliation: Department of Pathology and Centre of Infection and Immunology, The University of Hong Kong, Hong Kong, China.
authors.forename: Liwei
authors.initials: L
authors.lastname: Lu
authors.affiliation: Department of Laboratory Medicine, The Affiliated People's Hospital, Jiangsu University, Zhenjiang, China; Institute of Laboratory Medicine, Jiangsu University School of Medical Science and Laboratory Medicine, Zhenjiang, China.
authors.forename: Huaxi
authors.initials: H
authors.lastname: Xu
authors.affiliation: Department of Laboratory Medicine, The Affiliated People's Hospital, Jiangsu University, Zhenjiang, China; Institute of Laboratory Medicine, Jiangsu University School of Medical Science and Laboratory Medicine, Zhenjiang, China.
authors.forename: Shengjun
authors.initials: S
authors.lastname: Wang
mesh_terms: D000273:Adipose Tissue / Q000276:immunology* / Q000473:pathology; D000818:Animals; D018920:Coculture Techniques; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000502:physiology*; D059305:Diet, High-Fat / Q000009:adverse effects; D018836:Inflammation Mediators / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000209:etiology / Q000276:immunology*; D058504:Th17 Cells / Q000502:physiology*
chemical_list: D016207:Cytokines; D018836:Inflammation Mediators