title: Identification and characterization of microRNAs expressed in chicken skeletal muscle.
abstract: MicroRNAs (miRNAs, miRs) encompass a class of small non-coding RNAs that often negatively regulate gene expression. miRNAs play an essential role in skeletal muscle, determining the proper development and maintenance of this tissue. In comparison to other organs and tissues, the full set of muscle miRNAs and its expression patterns are still poorly understood. In this report, a chicken skeletal muscle miRNA library was constructed, and the expression of selected miRNAs was further characterized during muscle development in chicken lines with distinct muscling phenotypes. Clone library sequence analysis revealed 40 small RNAs with similarities to previously described chicken miRNAs, seven miRNAs that were never identified before in chicken, and some sequence clusters representing other possible novel miRNAs. Temporal expression profiles of three miRNAs associated with cell proliferation and differentiation (miR-125b, miR-221, and miR-206) in two chicken lines (broiler and layer) revealed the differential steady-state levels of these miRs during skeletal muscle growth and suggests that miR-206 is involved in the muscling phenotype that is observed in growth-selected chicken lines.
authors.affiliation: Departamento de Zootecnia, Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, Piracicaba, SP, Brasil.
authors.forename: A P D
authors.initials: AP
authors.lastname: Andreote
authors.affiliation: Departamento de Zootecnia, Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, Piracicaba, SP, Brasil.
authors.forename: M F
authors.initials: MF
authors.lastname: Rosario
authors.affiliation: Embrapa Suínos e Aves, Concórdia, SC, Brasil.
authors.forename: M C
authors.initials: MC
authors.lastname: Ledur
authors.affiliation: Departamento de Zootecnia, Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, Piracicaba, SP, Brasil.
authors.forename: E C
authors.initials: EC
authors.lastname: Jorge
authors.affiliation: Beltsville Area Research Center, United Center Department of Agriculture, Beltsville, MD, USA.
authors.forename: T S
authors.initials: TS
authors.lastname: Sonstegard
authors.affiliation: Beltsville Area Research Center, United Center Department of Agriculture, Beltsville, MD, USA.
authors.forename: L
authors.initials: L
authors.lastname: Matukumalli
authors.affiliation: Departamento de Zootecnia, Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, Piracicaba, SP, Brasil llcoutinho@usp.br.
authors.forename: L L
authors.initials: LL
authors.lastname: Coutinho
mesh_terms: D000818:Animals; D001483:Base Sequence; D002645:Chickens; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D018507:Gene Expression Regulation, Developmental; D035683:MicroRNAs / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D024510:Muscle Development / Q000235:genetics; D018482:Muscle, Skeletal / Q000378:metabolism*; D009690:Nucleic Acid Conformation; D016415:Sequence Alignment
chemical_list: D035683:MicroRNAs