title: The effect of CYP1A1 and GSTP1 isozymes on the occurrence of aortic aneurysms.
abstract: BACKGROUND
  Aortic aneurysms are vascular diseases that are associated with high mortality and morbidity. Cytochrome P450 CYP1A1 and glutathione S-transferase (GSTP1) isozymes were searched and compared with the patients who had experienced aortic surgery due to aortic aneurysm and atherosclerotic patients without aneurysm to find the relation of the oxidative stress with the aneurysms.
  
  
  METHODS
  Study group consisted of the patients with the diagnosis of aortic aneurysm (group I, n: 12) and control group who were operated for coronary bypass surgery: preoperatively drug users (group II, n: 21) and nonusers (group III, n: 15). Paraffin sections (4 μm thick) of aortic biopsy materials were stained with hematoxylin and eosine, CYP1A1 and GSTP1 immunohistochemical markers. The specimens were evaluated using light microscopy at 40- to 400-fold magnification.
  
  
  RESULTS
  The expressions of CYP1A1 and GSTP1 isozymes were found statistically significantly higher in the patients who have an aortic aneurysm than both the control groups (p < 0.05). There was no significant association between protein expressions, drugs and duration of usage, patient's demographic variables, and smoking (p > 0.05).
  
  
  CONCLUSIONS
  In this pioneering study, CYP1A1 and GSTP1 isozymes are related with the aneurysms. The strategy that prevents the oxidative stress for the patients who had aortic aneurysms could be a valuable choice of searching to effect the aneurysmal progression.
authors.affiliation: Department of Cardiovascular Surgery, Turkiye Yuksek Ihtisas Training and Research Hospital, Ankara, Turkey.
authors.forename: Erdal
authors.initials: E
authors.lastname: Simsek
authors.affiliation: Department of Milk and Dairy Products Technology, Pamukkale University Acipayam Vocational School, Denizli, Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Kilic
authors.affiliation: Department of Pathology, Kecioren Training and Research Hospital, Ankara, Turkey.
authors.forename: Gulcin
authors.initials: G
authors.lastname: Simsek
authors.affiliation: Department of Biology, Kirikkale University, Kirikkale, Turkey.
authors.forename: Serpil
authors.initials: S
authors.lastname: Oguztuzun
authors.affiliation: Department of Biology, Kirikkale University, Kirikkale, Turkey.
authors.forename: Busra
authors.initials: B
authors.lastname: Moran
authors.affiliation: Department of Cardiovascular Surgery, Turkiye Yuksek Ihtisas Training and Research Hospital, Ankara, Turkey.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Saritas
authors.affiliation: Department of Cardiovascular Surgery, Turkiye Yuksek Ihtisas Training and Research Hospital, Ankara, Turkey.
authors.forename: A Tulga
authors.initials: AT
authors.lastname: Ulus
mesh_terms: D000368:Aged; D001011:Aorta / Q000201:enzymology* / Q000473:pathology / Q000601:surgery; D001014:Aortic Aneurysm / Q000175:diagnosis / Q000201:enzymology* / Q000601:surgery; D001706:Biopsy; D016022:Case-Control Studies; D019363:Cytochrome P-450 CYP1A1 / Q000032:analysis*; D005260:Female; D051549:Glutathione S-Transferase pi / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D015854:Up-Regulation
chemical_list: C569463:CYP1A1 protein, human; D019363:Cytochrome P-450 CYP1A1; C496556:GSTP1 protein, human; D051549:Glutathione S-Transferase pi