title: Microbial communities from different types of natural wastewater treatment systems: vertical and horizontal flow constructed wetlands and biofilters.
abstract: The prokaryotic microbial communities (Bacteria and Archaea) of three different systems operating in Denmark for the treatment of domestic wastewater (horizontal flow constructed wetlands (HFCW), vertical flow constructed wetlands (VFCW) and biofilters (BF)) was analysed using endpoint PCR followed by Denaturing Gradient Gel Electrophoresis (DGGE). Further sequencing of the most representative bacterial bands revealed that diverse and distinct bacterial communities were found in each system unit, being γ-Proteobacteria and Bacteroidetes present mainly in all of them, while Firmicutes was observed in HFCW and BF. Members of the Actinobacteria group, although found in HFCW and VFCW, seemed to be more abundant in BF units. Finally, some representatives of α, β and δ-Proteobacteria, Acidobacteria and Chloroflexi were also retrieved from some samples. On the other hand, a lower archaeal diversity was found in comparison with the bacterial population. Cluster analysis of the DGGE bacterial band patterns showed that community structure was related to the design of the treatment system and the organic matter load, while no clear relation was established between the microbial assemblage and the wastewater influent.
authors.affiliation: Health and Environmental Microbiology Laboratory, Optics and Optometry Department & AQUASOST - UNESCO Chair on Sustainability, Universitat Politècnica de Catalunya, Edifici Gaia, Pg. Ernest Lluch/Rambla Sant Nebridi, Terrassa 08222, Spain. Electronic address: jordi.morato@upc.edu.
authors.forename: B
authors.initials: B
authors.lastname: Adrados
authors.affiliation: Departament de Genètica i Microbiologia, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain.
authors.forename: O
authors.initials: O
authors.lastname: Sánchez
authors.affiliation: Aarhus University, Department of Bioscience, Ole Worms Allé 1, Building 1135, 8000 Århus C, Denmark.
authors.forename: C A
authors.initials: CA
authors.lastname: Arias
authors.affiliation: Facultad de Ciencias Biológicas y Ambientales, Universidad de León, Campus de Vegazana s/n, 24071 León, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Becares
authors.affiliation: Departament de Genètica i Microbiologia, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain.
authors.forename: L
authors.initials: L
authors.lastname: Garrido
authors.affiliation: Departament de Genètica i Microbiologia, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Mas
authors.affiliation: Aarhus University, Department of Bioscience, Ole Worms Allé 1, Building 1135, 8000 Århus C, Denmark.
authors.forename: H
authors.initials: H
authors.lastname: Brix
authors.affiliation: Health and Environmental Microbiology Laboratory, Optics and Optometry Department & AQUASOST - UNESCO Chair on Sustainability, Universitat Politècnica de Catalunya, Edifici Gaia, Pg. Ernest Lluch/Rambla Sant Nebridi, Terrassa 08222, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Morató
mesh_terms: D019149:Bioreactors; D058645:Denaturing Gradient Gel Electrophoresis; D005374:Filtration; D016133:Polymerase Chain Reaction; D014865:Waste Disposal, Fluid / Q000379:methods*; D014871:Water Microbiology; D053833:Wetlands
keywords: Biofilters; Constructed wetlands; Domestic wastewater; Microbial community; PCR-DGGE