title: Primary cultured astrocytes from old rats are capable to activate the Nrf2 response against MPP+ toxicity after tBHQ pretreatment.
abstract: Astrocytes are key players for brain physiology, protecting neurons by releasing antioxidant enzymes; however, they are also susceptible to damage by neurotoxins. Nuclear factor erythroid-derived 2-like 2 (Nrf2) is a central regulator of the antioxidant response, and therefore, pharmacologic inducers are often used to activate this transcription factor to induce cellular protection. To date, it still remains unknown if cells from aged animals are capable of developing this response. Therefore, the purpose of this work was to determine if cortical astrocytes derived from old rats are able to respond to tertbuthyl-hydroquinene (tBHQ) pretreatment and stimulate the Nrf2-antioxidant response pathway to induce an antioxidant strategy against MPP+ toxicity, one of the most used molecules to model Parkinson's disease. Our results show that, although astrocytes from adult and old rats were more susceptible to MPP+ toxicity than astrocytes from newborn rats, when pretreated with tertbuthyl-hydroquinene, they were able to transactivate Nrf2, increasing antioxidant enzymes and developing cellular protection. These results are discussed in terms of the doses used to create protective responses.
authors.affiliation: Departamento de Ciencias de la Salud, DCBS, Universidad Autónoma Metropolitana Iztapalapa, ciudad de México, México.
authors.forename: Adriana
authors.initials: A
authors.lastname: Alarcón-Aguilar
authors.affiliation: Area de Ciencia Básica, Instituto Nacional de Geriatría, SSA, ciudad de México, Mexico.
authors.forename: Armando
authors.initials: A
authors.lastname: Luna-López
authors.affiliation: Departamento de Medicina Genómica y Toxicología Ambiental, IIB, UNAM, ciudad de México, México; Departamento de Bioquímica, INCMNZS, ciudad de México, México.
authors.forename: José L
authors.initials: JL
authors.lastname: Ventura-Gallegos
authors.affiliation: Departamento de Ciencias de la Salud, DCBS, Universidad Autónoma Metropolitana Iztapalapa, ciudad de México, México.
authors.forename: Roberto
authors.initials: R
authors.lastname: Lazzarini
authors.affiliation: Departamento de Neuroquímica, Instituto Nacional de Neurología y Neurocirugía, SSA, ciudad de México, México.
authors.forename: Sonia
authors.initials: S
authors.lastname: Galván-Arzate
authors.affiliation: Departamento de Ciencias de la Salud, DCBS, Universidad Autónoma Metropolitana Iztapalapa, ciudad de México, México.
authors.forename: Viridiana Y
authors.initials: VY
authors.lastname: González-Puertos
authors.affiliation: División de Neurociencias, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, ciudad de México, México.
authors.forename: Julio
authors.initials: J
authors.lastname: Morán
authors.affiliation: Laboratorio de Aminoácidos Excitadores, Instituto Nacional de Neurología y Neurocirugía, SSA, México, México.
authors.forename: Abel
authors.initials: A
authors.lastname: Santamaría
authors.affiliation: Departamento de Ciencias de la Salud, DCBS, Universidad Autónoma Metropolitana Iztapalapa, ciudad de México, México. Electronic address: mkf@xanum.uam.mx.
authors.forename: Mina
authors.initials: M
authors.lastname: Königsberg
mesh_terms: D015655:1-Methyl-4-phenylpyridinium / Q000633:toxicity*; D000818:Animals; D001253:Astrocytes / Q000187:drug effects* / Q000378:metabolism*; D002478:Cells, Cultured; D005625:Frontal Lobe / Q000166:cytology; D006873:Hydroquinones / Q000494:pharmacology*; D051267:NF-E2-Related Factor 2 / Q000378:metabolism* / Q000502:physiology; D009498:Neurotoxins / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D006873:Hydroquinones; D051267:NF-E2-Related Factor 2; D009498:Neurotoxins; C495637:Nfe2l2 protein, rat; C018855:2-tert-butylhydroquinone; D015655:1-Methyl-4-phenylpyridinium
keywords: Aging; Antioxidant enzymes; Astrocytes; GSH; MPP+; Nrf2; Oxidative stress; Primary cultures; tBHQ