title: Enhanced cholesterol efflux to HDL through the ABCA1 transporter in hypertriglyceridemia of type 2 diabetes.
abstract: OBJECTIVE
  Our objective was to examine the role of hypertriglyceridemia on the capacity of HDL to facilitate ABCA-1 mediated cholesterol efflux in type 2 diabetes (T2DM).
  
  
  METHODS
  HDL mediated cholesterol efflux through the ABCA-1 transporter was measured using BHK cell lines in samples of 71 participants with T2DM in the presence or absence of high triglyceride levels (TG). Additionally, HDL mediated efflux was measured in 13 diabetic and non-diabetic participants fasting and four hours after a high-fat test challenge.
  
  
  RESULTS
  HDL mediated cholesterol efflux function was increased in participants with T2DM with hypertriglyceridemia when compared to participants with T2DM without hypertriglyceridemia (efflux ratio mean±standard deviation (SD), T2DM+TG: 1.17±0.25 vs. T2DM - TG: 1.03±0.19, p=0.0098). In the fat challenge study, we observed a significant increase in ABCA-1 mediated cholesterol efflux capacity following an ingestion of high-fat test meal by participants in both groups of T2DM (n=6, efflux ratio, mean±SD, pre: 0.86±0.4 vs. post: 1.34±0.6, p=0.01) and non-diabetic participants (n=7, efflux ratio mean±SD pre: 1.24±0.31 vs. post: 1.39±0.42, p=0.04) that was partly explained by the difference in CETP activity (r=0.6, p=0.03).
  
  
  CONCLUSIONS
  Our study suggests that high triglyceride levels facilitate ABCA-1 mediated efflux function of HDL in part by activating CETP.
authors.affiliation: University of Southern California, Los Angeles, CA. Electronic address: hyassine@usc.edu.
authors.forename: Hussein N
authors.initials: HN
authors.lastname: Yassine
authors.affiliation: University of Southern California, Los Angeles, CA.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Belopolskaya
authors.affiliation: University of Arizona, Tucson, AZ.
authors.forename: Christina
authors.initials: C
authors.lastname: Schall
authors.affiliation: University of Arizona, Tucson, AZ; Southern Arizona VA Health Care Systems, Tucson, AZ.
authors.forename: Craig S
authors.initials: CS
authors.lastname: Stump
authors.affiliation: University of Arizona, Tucson, AZ; Southwest Environmental Health Sciences Center, Tucson, AZ.
authors.forename: Serrine S
authors.initials: SS
authors.lastname: Lau
authors.affiliation: Phoenix VA Health Care System, Phoenix, AZ.
authors.forename: Peter D
authors.initials: PD
authors.lastname: Reaven
mesh_terms: D064286:ATP Binding Cassette Transporter 1 / Q000378:metabolism / Q000502:physiology*; D000368:Aged; D000818:Animals; D001692:Biological Transport; D002478:Cells, Cultured; D002784:Cholesterol / Q000378:metabolism*; D053480:Cholesterol Ester Transfer Proteins / Q000378:metabolism; D006224:Cricetinae; D003430:Cross-Sectional Studies; D003924:Diabetes Mellitus, Type 2 / Q000150:complications / Q000378:metabolism*; D005260:Female; D006801:Humans; D015228:Hypertriglyceridemia / Q000209:etiology / Q000378:metabolism*; D008075:Lipoproteins, HDL / Q000378:metabolism*; D008297:Male; D008875:Middle Aged
chemical_list: C577836:ABCA1 protein, human; D064286:ATP Binding Cassette Transporter 1; C506642:CETP protein, human; D053480:Cholesterol Ester Transfer Proteins; D008075:Lipoproteins, HDL; D002784:Cholesterol
keywords: ABCA-1; CETP; Diabetes; Efflux; HDL; Triglycerides