title: Upregulation of RECK gene expression by small double-stranded RNA targeting the promoter region.
abstract: Recent studies have demonstrated that small double-stranded RNAs (dsRNAs) complementary to the promoter region of target genes enhance the expression of those genes following transfection into cells. Here we show that expression of the matrix metalloproteinase (MMP) inhibitor RECK is activated in the cultured tumor cell lines by transfection with dsRNA complementary to the promoter of the RECK gene, leading to suppression of the expression of MMPs and it inhibited tumor cell invasion. These results support the suggestion that dsRNA complementary to the promoter region of tumor suppressor genes would have potential as a novel antitumor agent.
authors.affiliation: 1] Laboratory of Biochemistry and Molecular Biology, Graduate School of Pharmaceutical Sciences, Osaka Univeristy, Osaka, Japan [2] Laboratory of Regulatory Sciences for Oligonucleotide Therapeutics, Clinical Drug Development Unit, Graduate School of Pharmaceutical Sciences, Osaka University, Osaka, Japan.
authors.forename: F
authors.initials: F
authors.lastname: Sakurai
authors.affiliation: Laboratory of Biochemistry and Molecular Biology, Graduate School of Pharmaceutical Sciences, Osaka Univeristy, Osaka, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Nanjo
authors.affiliation: Laboratory of Biochemistry and Molecular Biology, Graduate School of Pharmaceutical Sciences, Osaka Univeristy, Osaka, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Okamoto
authors.affiliation: Laboratory of Biochemistry and Molecular Biology, Graduate School of Pharmaceutical Sciences, Osaka Univeristy, Osaka, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Tachibana
authors.affiliation: 1] Laboratory of Biochemistry and Molecular Biology, Graduate School of Pharmaceutical Sciences, Osaka Univeristy, Osaka, Japan [2] Laboratory of Hepatic Differentiation Research, National Institute of Biomedical Innovation, Osaka, Japan [3] Center for Advanced Medical Engineering and Informatics, Osaka University, Osaka, Japan [4] Laboratory of iPS Research, Graduate School of Pharmaceutical Sciences, Osaka University, Osaka, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Mizuguchi
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000628:therapy; D000077192:Adenocarcinoma of Lung; D001483:Base Sequence; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000628:therapy; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000628:therapy; D045744:Cell Line, Tumor; D005260:Female; D058851:GPI-Linked Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D015316:Genetic Therapy / Q000379:methods*; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000628:therapy; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000235:genetics; D014162:Transfection / Q000379:methods; D015854:Up-Regulation
chemical_list: D058851:GPI-Linked Proteins; C115460:RECK protein, human; D012330:RNA, Double-Stranded