title: [A method to determine the structure of the complex of enzyme and its substrate using 6-phosphate-beta-glucosidase at low temperature].
abstract: To capture a state of the enzyme in complex with an intact substrate, we developed and adopted a novel freezing method in crystal preparation procedure. Neither the elimination of the catalytically indispensable ligands, nor mutation or modification of the active site is required. At -20 degrees C, we soaked the crystal of 6-phosphate-beta-glucosidase (Bg1A) in the liquor containing p-nitrophenyl-beta-D-glucopyranoside-6-phosphate (pNPbetaG6P). The diffraction data at 2 A resolution was collected and an intact and unambiguous electron density map of pNPbetaG6P was obtained. These results provide an effective method for the research of cryoenzymology and the intermediate state of enzyme-substrate complex in the future.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Yiwei
authors.initials: Y
authors.lastname: Liu
authors.forename: Hongbin
authors.initials: H
authors.lastname: Li
mesh_terms: D003080:Cold Temperature; D003460:Crystallization; D005959:Glucosidases / Q000737:chemistry* / Q000378:metabolism*; D013379:Substrate Specificity; D014961:X-Ray Diffraction
chemical_list: D005959:Glucosidases; C066586:6-phospho-beta-glucosidase