title: Techniques for the production, isolation, and analysis of iron-sulfur proteins.
abstract: Iron-sulfur clusters constitute a group of cofactors found in many proteins that play key roles in an exceptionally wide range of metabolic processes. The chemical reactivity of iron-sulfur clusters means that they can be particularly prone to damage when removed from the protective environment of the cell. In general, the key to obtaining an intact, biologically active iron-sulfur cluster-containing protein is to maintain a strictly anaerobic environment throughout the entire process of protein purification and analysis. For many proteins, particularly those with more labile clusters, it is essential.
authors.affiliation: School of Chemistry, University of East Anglia, Norwich Research Park, Norwich, NR4 7TJ, UK, j.crack@uea.ac.uk.
authors.forename: Jason C
authors.initials: JC
authors.lastname: Crack
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Green
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Thomson
authors.forename: Nick E
authors.initials: NE
authors.lastname: Le Brun
mesh_terms: D000693:Anaerobiosis / Q000187:drug effects; D001671:Biochemistry / Q000379:methods*; D007501:Iron / Q000032:analysis; D007506:Iron-Sulfur Proteins / Q000096:biosynthesis* / Q000302:isolation & purification*; D010100:Oxygen / Q000494:pharmacology; D012015:Reference Standards; D013440:Sulfides / Q000378:metabolism; D017186:Titrimetry
chemical_list: D007506:Iron-Sulfur Proteins; D013440:Sulfides; D007501:Iron; D010100:Oxygen