title: Immunogenicity of Escherichia coli expressed envelope 2 protein of Chikungunya virus.
abstract: Chikungunya fever, a re-emerging infection, is an arthropod-borne viral disease prevalent in different parts of the world, particularly Africa and South East Asia. Chikungunya virus envelope 2 protein is involved in binding to host receptors and it contains specific epitopes that elicit virus neutralizing antibodies. A highly immunogenic, recombinant Chikungunya virus envelope 2 protein was produced by bioreactor in Escherichia coli for development of a suitable diagnostic and vaccine candidate. This protein was refolded and further purified to achieve biologically active protein. The biological function of refolded and purified recombinant envelope 2 protein of Chikungunya virus was confirmed by its ability to generate envelope 2 specific antibodies with high titers in animal models. These findings suggest that recombinant envelope 2 protein of Chikungunya virus in combination with compatible adjuvant is highly immunogenic. Thus, recombinant envelope 2 protein can be a potential diagnostic reagent and vaccine candidate against Chikungunya virus infection.
authors.affiliation: Bioprocess Scale up Facility; Defense Research and Development Establishment; Gwalior, India.
authors.forename: Nagesh K
authors.initials: NK
authors.lastname: Tripathi
authors.affiliation: Division of Virology; Defense Research and Development Establishment; Gwalior, India.
authors.forename: Raj
authors.initials: R
authors.lastname: Priya
authors.affiliation: Division of Virology; Defense Research and Development Establishment; Gwalior, India.
authors.forename: Ambuj
authors.initials: A
authors.lastname: Shrivastava
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis* / Q000097:blood; D061249:Batch Cell Culture Techniques; D019149:Bioreactors; D065632:Chikungunya Fever / Q000276:immunology / Q000517:prevention & control*; D002646:Chikungunya virus / Q000737:chemistry* / Q000276:immunology; D003470:Culture Media; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005163:Factor Analysis, Statistical; D005285:Fermentation; D005620:Freund's Adjuvant / Q000008:administration & dosage; D015870:Gene Expression; D058849:Protein Refolding; D011817:Rabbits; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D014759:Viral Envelope Proteins / Q000008:administration & dosage / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D003470:Culture Media; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins; D014765:Viral Vaccines; D005620:Freund's Adjuvant
keywords: Chikungunya; Escherichia coli; Plackett-Burman design; fermentation; immunogenicity; vaccine; virus