title: Structure determination of BA0150, a putative polysaccharide deacetylase from Bacillus anthracis.
abstract: Polysaccharide deacetylases are bacterial enzymes that catalyze the deacetylation of acetylated sugars on the membranes of Gram-positive bacteria, allowing them to be unrecognized by host immune systems. Inhibition of these enzymes would disrupt such pathogenic defensive mechanisms and therefore offers a promising route for the development of novel antibiotic therapeutics. Here, the first X-ray crystal structure of BA0150, a putative polysaccharide deacetylase from Bacillus anthracis, is reported to 2.0 Å resolution. The overall structure maintains the conserved (α/β)8 fold that is characteristic of this family of enzymes. The lack of a catalytic metal ion and a distinctive metal-binding site, however, suggest that this enzyme is not a functional polysaccharide deacetylase.
authors.affiliation: Department of Chemistry, Ithaca College, 953 Danby Road, Ithaca, NY 14850, USA.
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Strunk
authors.affiliation: Department of Chemistry, Ithaca College, 953 Danby Road, Ithaca, NY 14850, USA.
authors.forename: Katrina M
authors.initials: KM
authors.lastname: Piemonte
authors.affiliation: Department of Chemistry, Ithaca College, 953 Danby Road, Ithaca, NY 14850, USA.
authors.forename: Natasha M
authors.initials: NM
authors.lastname: Petersen
authors.affiliation: Department of Biology, Enzyme Biotechnology Group, University of Crete, PO Box 2209, Vassilika Vouton, 71409 Heraklion, Crete, Greece.
authors.forename: Dimitris
authors.initials: D
authors.lastname: Koutsioulis
authors.affiliation: Department of Biology, Enzyme Biotechnology Group, University of Crete, PO Box 2209, Vassilika Vouton, 71409 Heraklion, Crete, Greece.
authors.forename: Vassilis
authors.initials: V
authors.lastname: Bouriotis
authors.affiliation: Northeastern Collaborative Access Team (NE-CAT) and Department of Chemistry and Chemical Biology, Cornell University, Building 436E, Argonne National Laboratory, 9700 South Cass Avenue, Argonne, IL 60439, USA.
authors.forename: Kay
authors.initials: K
authors.lastname: Perry
authors.affiliation: Department of Chemistry, Ithaca College, 953 Danby Road, Ithaca, NY 14850, USA.
authors.forename: Kathryn E
authors.initials: KE
authors.lastname: Cole
mesh_terms: D000581:Amidohydrolases / Q000737:chemistry*; D000595:Amino Acid Sequence; D001408:Bacillus anthracis / Q000201:enzymology*; D018360:Crystallography, X-Ray; D008969:Molecular Sequence Data; D011487:Protein Conformation; D017386:Sequence Homology, Amino Acid; D013052:Spectrometry, X-Ray Emission
chemical_list: D000581:Amidohydrolases; C022427:polysaccharide deacetylase
keywords: Bacillus anthracis; carbohydrate esterases; polysaccharide deacetylases