title: Detection of haplotype mutations of the MD-2 gene promoter associated with Der p2-induced allergy using a nanostructured biosensor.
abstract: Group 2 allergens (Der p2) have been reported to be a major cause of the human immune response to dust mite allergens. In this study, we have demonstrated for the first time the effective differentiation between haplotype mutation and normal genes in the MD-2 gene promoter using a nanostructured biosensor. A 70-mer gene fragment containing the haplotype of two single nucleotide polymorphisms in the MD-2 gene promoter region was used as a probe to detect haplotype mutations associated with Der p2-induced allergy. Discrimination was achieved using electrochemical impedance spectroscopy. The discrimination experiments employed 30 haplotype mutation samples and 30 normal target samples. The haplotype mutation samples and normal target samples could be clearly discriminated, even using samples produced by a five-cycle polymerase chain reaction process. The time and cost of sample preparation for the polymerase chain reaction process in the clinical setting can thus be reduced.
authors.affiliation: Department of Mechanical Engineering, National Chung-Hsing University, Taichung, Taiwan.
authors.forename: Yu-Ting
authors.initials: YT
authors.lastname: Chin
authors.affiliation: Department of Medical Education and Research, Taichung Veterans General Hospital, Taichung, Taiwan.
authors.forename: En-Chih
authors.initials: EC
authors.lastname: Liao
authors.affiliation: Department of Mechanical Engineering, National Chung-Hsing University, Taichung, Taiwan.
authors.forename: Chia-Che
authors.initials: CC
authors.lastname: Wu
authors.affiliation: Department of Mechanical Engineering, National Chung-Hsing University, Taichung, Taiwan ; Graduate Institute of Biomedical Engineering, Taichung, Taiwan ; PhD Program in Tissue Engineering and Regenerative Medicine, National Chung-Hsing University, Taichung, Taiwan.
authors.forename: Gou-Jen
authors.initials: GJ
authors.lastname: Wang
authors.affiliation: Department of Medical Education and Research, Taichung Veterans General Hospital, Taichung, Taiwan.
authors.forename: Jaw-Ji
authors.initials: JJ
authors.lastname: Tsai
mesh_terms: D000485:Allergens; D039741:Antigens, Dermatophagoides; D060829:Arthropod Proteins; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D004252:DNA Mutational Analysis; D015342:DNA Probes / Q000235:genetics; D058266:Dielectric Spectroscopy; D006046:Gold; D006239:Haplotypes; D006801:Humans; D006967:Hypersensitivity / Q000209:etiology / Q000235:genetics*; D051218:Lymphocyte Antigen 96 / Q000235:genetics*; D053768:Metal Nanoparticles / Q000648:ultrastructure; D009154:Mutation; D050997:Nanomedicine; D036103:Nanotechnology; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic
chemical_list: D000485:Allergens; D039741:Antigens, Dermatophagoides; D060829:Arthropod Proteins; D015342:DNA Probes; C451163:Dermatophagoides pteronyssinus antigen p 2; C495377:LY96 protein, human; D051218:Lymphocyte Antigen 96; D006046:Gold
keywords: MD-2 gene promoter; electrochemical impedance spectroscopy; haplotype mutation detection; nanostructured biosensor