title: Identification of the proteins related to SET-mediated hepatic cytotoxicity of trichloroethylene by proteomic analysis.
abstract: Trichloroethylene (TCE) is an effective solvent for a variety of organic materials. Since the wide use of TCE as industrial degreasing of metals, adhesive paint and polyvinyl chloride production, TCE has turned into an environmental and occupational toxicant. Exposure to TCE could cause severe hepatotoxicity; however, the toxic mechanisms of TCE remain poorly understood. Recently, we reported that SET protein mediated TCE-induced cytotoxicity in L-02 cells. Here, we further identified the proteins related to SET-mediated hepatic cytotoxicity of TCE using the techniques of DIGE (differential gel electrophoresis) and MALDI-TOF-MS/MS. Among the 20 differential proteins identified, 8 were found to be modulated by SET in TCE-induced cytotoxicity and three of them (cofilin-1, peroxiredoxin-2 and S100-A11) were validated by Western-blot analysis. The functional analysis revealed that most of the identified SET-modulated proteins are apoptosis-associated proteins. These data indicated that these proteins may be involved in SET-mediated hepatic cytotoxicity of TCE in L-02 cells.
authors.affiliation: Key Laboratory of Modern Toxicology of Shenzhen, Medical Key Laboratory of Guangdong Province, Medical Key Laboratory of Health Toxicology of Shenzhen, Shenzhen Center for Disease Control and Prevention, No 8 Longyuan Road, Nanshan District, Shenzhen 518055, China.
authors.forename: Xiaohu
authors.initials: X
authors.lastname: Ren
authors.affiliation: Key Laboratory of Modern Toxicology of Shenzhen, Medical Key Laboratory of Guangdong Province, Medical Key Laboratory of Health Toxicology of Shenzhen, Shenzhen Center for Disease Control and Prevention, No 8 Longyuan Road, Nanshan District, Shenzhen 518055, China.
authors.forename: Xifei
authors.initials: X
authors.lastname: Yang
authors.affiliation: Key Laboratory of Modern Toxicology of Shenzhen, Medical Key Laboratory of Guangdong Province, Medical Key Laboratory of Health Toxicology of Shenzhen, Shenzhen Center for Disease Control and Prevention, No 8 Longyuan Road, Nanshan District, Shenzhen 518055, China.
authors.forename: Wen-Xu
authors.initials: WX
authors.lastname: Hong
authors.affiliation: Key Laboratory of Modern Toxicology of Shenzhen, Medical Key Laboratory of Guangdong Province, Medical Key Laboratory of Health Toxicology of Shenzhen, Shenzhen Center for Disease Control and Prevention, No 8 Longyuan Road, Nanshan District, Shenzhen 518055, China.
authors.forename: Peiwu
authors.initials: P
authors.lastname: Huang
authors.affiliation: School of Life Sciences, Shenzhen University, Nanhai Avenue 3688, Shenzhen 518060, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of Modern Toxicology of Shenzhen, Medical Key Laboratory of Guangdong Province, Medical Key Laboratory of Health Toxicology of Shenzhen, Shenzhen Center for Disease Control and Prevention, No 8 Longyuan Road, Nanshan District, Shenzhen 518055, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: Key Laboratory of Modern Toxicology of Shenzhen, Medical Key Laboratory of Guangdong Province, Medical Key Laboratory of Health Toxicology of Shenzhen, Shenzhen Center for Disease Control and Prevention, No 8 Longyuan Road, Nanshan District, Shenzhen 518055, China.
authors.forename: Jinbo
authors.initials: J
authors.lastname: Ye
authors.affiliation: Key Laboratory of Modern Toxicology of Shenzhen, Medical Key Laboratory of Guangdong Province, Medical Key Laboratory of Health Toxicology of Shenzhen, Shenzhen Center for Disease Control and Prevention, No 8 Longyuan Road, Nanshan District, Shenzhen 518055, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Huang
authors.affiliation: Key Laboratory of Modern Toxicology of Shenzhen, Medical Key Laboratory of Guangdong Province, Medical Key Laboratory of Health Toxicology of Shenzhen, Shenzhen Center for Disease Control and Prevention, No 8 Longyuan Road, Nanshan District, Shenzhen 518055, China.
authors.forename: Xinfeng
authors.initials: X
authors.lastname: Huang
authors.affiliation: School of Life Sciences, Shenzhen University, Nanhai Avenue 3688, Shenzhen 518060, China.
authors.forename: Liming
authors.initials: L
authors.lastname: Shen
authors.affiliation: Key Laboratory of Modern Toxicology of Shenzhen, Medical Key Laboratory of Guangdong Province, Medical Key Laboratory of Health Toxicology of Shenzhen, Shenzhen Center for Disease Control and Prevention, No 8 Longyuan Road, Nanshan District, Shenzhen 518055, China.
authors.forename: Linqing
authors.initials: L
authors.lastname: Yang
authors.affiliation: Key Laboratory of Modern Toxicology of Shenzhen, Medical Key Laboratory of Guangdong Province, Medical Key Laboratory of Health Toxicology of Shenzhen, Shenzhen Center for Disease Control and Prevention, No 8 Longyuan Road, Nanshan District, Shenzhen 518055, China.
authors.forename: Zhixiong
authors.initials: Z
authors.lastname: Zhuang
authors.affiliation: Key Laboratory of Modern Toxicology of Shenzhen, Medical Key Laboratory of Guangdong Province, Medical Key Laboratory of Health Toxicology of Shenzhen, Shenzhen Center for Disease Control and Prevention, No 8 Longyuan Road, Nanshan District, Shenzhen 518055, China. Electronic address: junii8@126.com.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Liu
mesh_terms: D018685:Anesthetics, Inhalation / Q000009:adverse effects; D017209:Apoptosis / Q000187:drug effects; D015153:Blotting, Western; D002460:Cell Line; D056486:Chemical and Drug Induced Liver Injury / Q000378:metabolism; D051338:Cofilin 1 / Q000378:metabolism*; D004268:DNA-Binding Proteins; D004785:Environmental Pollutants / Q000633:toxicity; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism; D056488:Histone Chaperones / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D054464:Peroxiredoxins / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D034622:RNA Interference; D034741:RNA, Small Interfering; D009418:S100 Proteins / Q000378:metabolism*; D012997:Solvents / Q000633:toxicity*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry; D014157:Transcription Factors / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D014241:Trichloroethylene / Q000633:toxicity*; D057825:Two-Dimensional Difference Gel Electrophoresis
chemical_list: D018685:Anesthetics, Inhalation; C495810:CFL1 protein, human; D051338:Cofilin 1; D004268:DNA-Binding Proteins; D004785:Environmental Pollutants; D056488:Histone Chaperones; D034741:RNA, Small Interfering; D009418:S100 Proteins; C513473:SET protein, human; D012997:Solvents; D014157:Transcription Factors; C072337:S100A11 protein, human; D014241:Trichloroethylene; C086065:PRDX2 protein, human; D054464:Peroxiredoxins
keywords: Difference gel electrophoresis (DIGE); SET protein; Trichloroethylene (TCE); siRNA