title: Meniscus regeneration by syngeneic, minor mismatched, and major mismatched transplantation of synovial mesenchymal stem cells in a rat model.
abstract: We compared the effect of syngeneic and allogeneic transplantation of synovial mesenchymal stem cells (MSCs) for meniscus regeneration in a rat model. Synovium was harvested from the knee joints of three strains of rats. The anterior half of the medial meniscus in both knees of F344 rats was removed and 5 million synovial MSCs derived from F344 (syngeneic transplantation), Lewis (minor mismatched transplantation), and ACI (major mismatched transplantation) were injected into the knee of the F344 rats. At 4 weeks, the area of the regenerated meniscus in the F344 group was significantly larger than that in the ACI group. Histological score was significantly better in the F344 and Lewis groups than in the ACI group at 8 weeks. DiI labeled cells could be observed in the knee joint in the F344 group, but were hardly detected in the ACI group at 1 week. The number of macrophages and CD8 T cells at synovium around the meniscus defect was significantly lower in the F344 group than in the ACI group at 1 week. Syngeneic and minor mismatched transplantation of synovial MSCs promoted meniscus regeneration better than major mismatched transplantation in a rat meniscectmized model.
authors.affiliation: Department of Joint Surgery and Sports Medicine, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan; Department of Orthopaedic Surgery, Hyogo College of Medicine, Tokyo, Japan.
authors.forename: Makiko
authors.initials: M
authors.lastname: Okuno
authors.forename: Takeshi
authors.initials: T
authors.lastname: Muneta
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Koga
authors.forename: Nobutake
authors.initials: N
authors.lastname: Ozeki
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Nakagawa
authors.forename: Kunikazu
authors.initials: K
authors.lastname: Tsuji
authors.forename: Shinichi
authors.initials: S
authors.lastname: Yoshiya
authors.forename: Ichiro
authors.initials: I
authors.lastname: Sekiya
mesh_terms: D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology; D002454:Cell Differentiation; D007719:Knee Joint / Q000502:physiology* / Q000601:surgery; D008297:Male; D008592:Menisci, Tibial / Q000502:physiology* / Q000601:surgery; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051381:Rats; D011912:Rats, Inbred ACI; D011916:Rats, Inbred F344; D011917:Rats, Inbred Lew; D012038:Regeneration; D013045:Species Specificity; D013583:Synovial Membrane / Q000166:cytology*; D014184:Transplantation, Homologous; D014185:Transplantation, Isogeneic; D014945:Wound Healing
keywords: allogeneic transplantation; meniscus regeneration; mesenchymal stem cells; syngeneic transplantation; synovium