title: The role of HuR in the post-transcriptional regulation of interleukin-3 in T cells.
abstract: Human Interleukin-3 (IL-3) is a lymphokine member of a class of transiently expressed mRNAs harboring Adenosine/Uridine-Rich Elements (ARE) in their 3' untranslated regions (3'-UTRs). The regulatory effects of AREs are often mediated by specific ARE-binding proteins (ARE-BPs). In this report, we show that the human IL-3 3'-UTR plays a post-transcriptional regulation role in two human transformed cell lines. More specifically, we demonstrate that the hIL-3 3'-UTR represses the translation of a luciferase reporter both in HeLa and Jurkat T-cells. These results also revealed that the hIL-3 3'-UTR-mediated translational repression is exerted by an 83 nt region comprised mainly by AREs and some non-ARE sequences. Moreover, electrophoretic mobility shift assays (EMSAs) and UV-crosslinking analysis show that this hIL-3 ARE-rich region recruits five specific protein complexes, including the ARE-BPs HuR and TIA-1. HuR binding to this ARE-rich region appears to be spatially modulated during T-cell activation. Together, these results suggest that HuR recognizes the ARE-rich region and plays a role in the IL-3 3'-UTR-mediated post-transcriptional control in T-cells.
authors.affiliation: University of Puerto Rico-Río Piedras, Department of Biology, College of Natural Sciences, San Juan, Puerto Rico.
authors.forename: José A
authors.initials: JA
authors.lastname: González-Feliciano
authors.affiliation: Department of Biochemistry, University of Puerto Rico-Medical Sciences, San Juan, Puerto Rico.
authors.forename: Marimar
authors.initials: M
authors.lastname: Hernández-Pérez
authors.affiliation: University of Puerto Rico-Río Piedras, Department of Biology, College of Natural Sciences, San Juan, Puerto Rico.
authors.forename: Luis A
authors.initials: LA
authors.lastname: Estrella
authors.affiliation: University of Puerto Rico-Río Piedras, Department of Biology, College of Natural Sciences, San Juan, Puerto Rico.
authors.forename: Daisy D
authors.initials: DD
authors.lastname: Colón-López
authors.affiliation: University of Puerto Rico-Río Piedras, Department of Biology, College of Natural Sciences, San Juan, Puerto Rico.
authors.forename: Armando
authors.initials: A
authors.lastname: López
authors.affiliation: Department of Biochemistry, University of Puerto Rico-Medical Sciences, San Juan, Puerto Rico.
authors.forename: Marina
authors.initials: M
authors.lastname: Martínez
authors.affiliation: University of Puerto Rico-Río Piedras, Department of Biology, College of Natural Sciences, San Juan, Puerto Rico.
authors.forename: Kirla R
authors.initials: KR
authors.lastname: Maurás-Rivera
authors.affiliation: University of Puerto Rico-Río Piedras, Department of Biology, College of Natural Sciences, San Juan, Puerto Rico.
authors.forename: Clarivel
authors.initials: C
authors.lastname: Lasalde
authors.affiliation: University of Puerto Rico-Río Piedras, Department of Biology, College of Natural Sciences, San Juan, Puerto Rico.
authors.forename: Daviana
authors.initials: D
authors.lastname: Martínez
authors.affiliation: University of Puerto Rico-Río Piedras, Department of Biology, College of Natural Sciences, San Juan, Puerto Rico.
authors.forename: Félix
authors.initials: F
authors.lastname: Araujo-Pérez
authors.affiliation: University of Puerto Rico-Río Piedras, Department of Biology, College of Natural Sciences, San Juan, Puerto Rico; Department of Biochemistry, University of Puerto Rico-Medical Sciences, San Juan, Puerto Rico; Molecular Sciences Research Building, San Juan, Puerto Rico.
authors.forename: Carlos I
authors.initials: CI
authors.lastname: González
mesh_terms: D020413:3' Untranslated Regions; D051959:ELAV Proteins / Q000502:physiology*; D000067780:ELAV-Like Protein 1; D024202:Electrophoretic Mobility Shift Assay; D006801:Humans; D007377:Interleukin-3 / Q000235:genetics* / Q000378:metabolism*; D019169:Jurkat Cells; D008213:Lymphocyte Activation; D039102:Poly(A)-Binding Proteins / Q000502:physiology; D016601:RNA-Binding Proteins / Q000502:physiology*; D000074019:T-Cell Intracellular Antigen-1; D014170:Transformation, Genetic
chemical_list: D020413:3' Untranslated Regions; D051959:ELAV Proteins; D000067780:ELAV-Like Protein 1; C098770:ELAVL1 protein, human; D007377:Interleukin-3; D039102:Poly(A)-Binding Proteins; D016601:RNA-Binding Proteins; D000074019:T-Cell Intracellular Antigen-1; C071558:TIA1 protein, human