title: The expression of MCP-1 and CCR2 in induced rats periapical lesions.
abstract: OBJECTIVE
  Monocyte chemotatic protein-1 (MCP-1) has been found to promote chemotaxis, differentiation, and activation of osteoclasts. The aim of this study is to detect and localize the expressions of MCP-1 and its receptor CCR2 in a rat model of apical periodontitis.
  
  
  METHODS
  After exposing the pulp of the first mandibular molars, 25 Wistar rats were killed on days 0, 7, 14, 21, 28, and 35. Rat jaws containing the first molar were obtained and routinely prepared for histological analysis, immunohistochemistry, enzyme histochemistry and immunofluorescence staining.
  
  
  RESULTS
  Lesions expanded from day 0 to day 28, and stabilized thereafter. Most of the MCP-1-positive cells were inflammatory cells, and their amount increased from day 7 to day 28. Aside from inflammatory cells, CCR2 was also detected in osteoclasts in the lesions. From day 7 to day 14, the number of osteoclasts increased. From day 21 to day 35, fewer osteoclasts could be observed, and the number of osteoclast was negatively correlated with positive MCP-1 expression.
  
  
  CONCLUSIONS
  These findings showed that MCP-1/CCR2 could be observed and might possibly be involved in the development of periapical lesions.
authors.affiliation: School and Hospital of Stomatology, Wuhan University, Wuhan, China.
authors.forename: Lingshuang
authors.initials: L
authors.lastname: Liu
authors.affiliation: School and Hospital of Stomatology, Wuhan University, Wuhan, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.affiliation: School and Hospital of Stomatology, Wuhan University, Wuhan, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wu
authors.affiliation: School and Hospital of Stomatology, Wuhan University, Wuhan, China; School and Hospital of Stomatology, Department of Conservative Dentistry and Endodontics, Wuhan University, Wuhan, China. Electronic address: phs301@vip.163.com.
authors.forename: Bin
authors.initials: B
authors.lastname: Peng
mesh_terms: D000818:Animals; D002452:Cell Count; D018932:Chemokine CCL2 / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D007150:Immunohistochemistry; D008297:Male; D010485:Periapical Periodontitis / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D054390:Receptors, CCR2 / Q000378:metabolism*
chemical_list: C491766:Ccl2 protein, rat; D018932:Chemokine CCL2; D054390:Receptors, CCR2
keywords: CCR2; MCP-1; Osteoclast; Periapical lesion