title: An electrochemical sensing platform based on local repression of electrolyte diffusion for single-step, reagentless, sensitive detection of a sequence-specific DNA-binding protein.
abstract: In this paper, we report for the first time an electrochemical biosensor for single-step, reagentless, and picomolar detection of a sequence-specific DNA-binding protein using a double-stranded, electrode-bound DNA probe terminally modified with a redox active label close to the electrode surface. This new methodology is based upon local repression of electrolyte diffusion associated with protein-DNA binding that leads to reduction of the electrochemical response of the label. In the proof-of-concept study, the resulting electrochemical biosensor was quantitatively sensitive to the concentrations of the TATA binding protein (TBP, a model analyte) ranging from 40 pM to 25.4 nM with an estimated detection limit of ∼10.6 pM (∼80 to 400-fold improvement on the detection limit over previous electrochemical analytical systems).
authors.affiliation: College of Chemistry and Bioengineering, Guilin University of Technology, Guilin 541004, China. zy_hnu@163.com Niejinfang@glut.edu.cn.
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhang
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.forename: Jinfang
authors.initials: J
authors.lastname: Nie
authors.forename: Fuyang
authors.initials: F
authors.lastname: Jiang
authors.forename: Caibin
authors.initials: C
authors.lastname: Zhou
authors.forename: Jiani
authors.initials: J
authors.lastname: Yang
authors.forename: Jinlong
authors.initials: J
authors.lastname: Fan
authors.forename: Jianping
authors.initials: J
authors.lastname: Li
mesh_terms: D004268:DNA-Binding Proteins / Q000737:chemistry*; D004058:Diffusion; D055664:Electrochemical Techniques / Q000295:instrumentation*; D004573:Electrolytes / Q000737:chemistry*; D007202:Indicators and Reagents / Q000737:chemistry*; D057230:Limit of Detection; D015203:Reproducibility of Results
chemical_list: D004268:DNA-Binding Proteins; D004573:Electrolytes; D007202:Indicators and Reagents