title: Methods for studying metabolism in Drosophila.
abstract: Recent research using Drosophila melanogaster has seen a resurgence in studies of metabolism and physiology. This review focuses on major methods used to conduct this work. These include protocols for dietary interventions, measurements of triglycerides, cholesterol, glucose, trehalose, and glycogen, stains for lipid detection, and the use of gas chromatography-mass spectrometry (GC-MS) to detect major polar metabolites. It is our hope that this will provide a useful framework for both new and current researchers in the field.
authors.affiliation: Department of Human Genetics, University of Utah School of Medicine, Salt Lake City, UT 84112-5330, USA.
authors.forename: Jason M
authors.initials: JM
authors.lastname: Tennessen
authors.affiliation: Department of Human Genetics, University of Utah School of Medicine, Salt Lake City, UT 84112-5330, USA.
authors.forename: William E
authors.initials: WE
authors.lastname: Barry
authors.affiliation: Department of Biochemistry and the Metabolomics Core Research Facility, University of Utah School of Medicine, Salt Lake City, UT 84112, USA.
authors.forename: James
authors.initials: J
authors.lastname: Cox
authors.affiliation: Department of Human Genetics, University of Utah School of Medicine, Salt Lake City, UT 84112-5330, USA. Electronic address: carl.thummel@genetics.utah.edu.
authors.forename: Carl S
authors.initials: CS
authors.lastname: Thummel
mesh_terms: D000818:Animals; D004330:Drosophila / Q000235:genetics / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D055432:Metabolomics
keywords: Drosophila; Metabolism; Metabolomics; Physiology