title: Sour cherry (Prunus cerasus) seed extract increases heme oxygenase-1 expression and decreases proinflammatory signaling in peripheral blood human leukocytes from rheumatoid arthritis patients.
abstract: Sour cherry seed extract (SCE) was evaluated for its capacity to inhibit lipopolysaccharide-treated human peripheral blood T cells expressing tumor necrosis factor-alpha, and the chemokine interleukin-8. Both proteins are diagnostic biomarkers for inflammatory pathologies. Peripheral blood leukocytes from 11 rheumatoid arthritis (RA) patients and 8 healthy control subjects were co-cultured for 24h in lipopolysaccharide and the extract, then evaluated by flow cytometry for T cell activation and by enzyme-linked immunoassay for lymphocyte-associated heme oxygenase-1 (HO-1) expression. There was a dose-dependent decrease in expression of the immunophenotypes: CD3+TNF-α+, and CD3+IL8+ in cultures from RA patients to a greater extent than in cells from healthy participants. These results suggest that the extract may have a modulatory roll in RA and other inflammatory disorders via the induction of HO-1, thus abating oxidative stress and strengthening regulation of pro-inflammatory signaling pathways.
authors.affiliation: Department of Medical Laboratory Sciences, Faculty of Allied Health Sciences, Kuwait University, Kuwait. Electronic address: fadia@hsc.edu.kw.
authors.forename: Fadia
authors.initials: F
authors.lastname: Mahmoud
authors.affiliation: Debrecen University, Faculty of Pharmacy, Hungary.
authors.forename: David
authors.initials: D
authors.lastname: Haines
authors.affiliation: Department of Medical Laboratory Sciences, Faculty of Allied Health Sciences, Kuwait University, Kuwait.
authors.forename: Rana
authors.initials: R
authors.lastname: Al-Awadhi
authors.affiliation: Department of Medical Laboratory Sciences, Faculty of Allied Health Sciences, Kuwait University, Kuwait.
authors.forename: Ali A
authors.initials: AA
authors.lastname: Dashti
authors.affiliation: Department of Medicine, Faculty of Medicine, Kuwait University, Kuwait.
authors.forename: Adel
authors.initials: A
authors.lastname: Al-Awadhi
authors.affiliation: Department of Medical Laboratory Sciences, Faculty of Allied Health Sciences, Kuwait University, Kuwait.
authors.forename: Basel
authors.initials: B
authors.lastname: Ibrahim
authors.affiliation: Department of Medical Laboratory Sciences, Faculty of Allied Health Sciences, Kuwait University, Kuwait.
authors.forename: Bashayer
authors.initials: B
authors.lastname: Al-Zayer
authors.affiliation: Debrecen University, Faculty of Pharmacy, Hungary.
authors.forename: Bela
authors.initials: B
authors.lastname: Juhasz
authors.affiliation: Debrecen University, Faculty of Pharmacy, Hungary.
authors.forename: Arpad
authors.initials: A
authors.lastname: Tosaki
mesh_terms: D000328:Adult; D001172:Arthritis, Rheumatoid / Q000276:immunology*; D017252:CD3 Complex / Q000276:immunology; D002478:Cells, Cultured; D005260:Female; D051547:Heme Oxygenase-1 / Q000276:immunology*; D006801:Humans; D016209:Interleukin-8 / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000276:immunology; D008070:Lipopolysaccharides; D008875:Middle Aged; D010936:Plant Extracts / Q000494:pharmacology*; D027861:Prunus; D012639:Seeds; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology
chemical_list: D017252:CD3 Complex; C508606:CXCL8 protein, human; D016209:Interleukin-8; D008070:Lipopolysaccharides; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; C496541:HMOX1 protein, human; D051547:Heme Oxygenase-1
keywords: HO-1; IL-8; Peripheral blood; Rheumatoid arthritis; Sour cherry kernel; TNF-α