title: Anticancer activity of binary toxins from Lysinibacillus sphaericus IAB872 against human lung cancer cell line A549.
abstract: The inhibitory effect of binary toxic (Bin) protein produced by Lysinibacillus sphaericus IAB872 on cell proliferation of human lung, liver, stomach and cervical tumor cell lines was assessed using MTT assay. The effect of Bin protein on A549 cell proliferation, apoptosis, cell cycle, migration and invasion were examined by MTT assay, Western blotting, Immunocytochemical staining, flow cytometry assay and wound-healing assay. Results showed that Bin protein inhibits proliferation of a range of human cancer cells in vitro. The anti-proliferative effect of Bin is associated with cell apoptosis as a result of an increased ratio of cellular Bax/bcl-2, up-regulated CyclinB1 and down-regulated Cdc25c expression, and its anti-proliferative action was associated with cell cycle arrest in the G2/M-phase. Bin protein could promote apoptosis and inhibit motility and invasion of A549 cancer cells. The anti-proliferative effect of Bin protein was associated with the induction of apoptotic cell death and cell cycle disruption. These results show that Bin protein has the potential to be developed as a chemotherapeutic agent by induction of human tumor cell apoptosis.
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Luo
authors.forename: Cuicui
authors.initials: C
authors.lastname: Liu
authors.forename: Ruijuan
authors.initials: R
authors.lastname: Zhang
authors.forename: Jianwei
authors.initials: J
authors.lastname: He
authors.forename: Bei
authors.initials: B
authors.lastname: Han
mesh_terms: D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001407:Bacillus / Q000737:chemistry*; D001427:Bacterial Toxins / Q000302:isolation & purification / Q000494:pharmacology*; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D004354:Drug Screening Assays, Antitumor; D006367:HeLa Cells; D006801:Humans
chemical_list: D000970:Antineoplastic Agents; D001427:Bacterial Toxins; C046134:binB protein, Bacillus sphaericus