title: Genomic DNA library preparation for resistance gene enrichment and sequencing (RenSeq) in plants.
abstract: Enrichment of genomic DNA for genes of interest prior to high-throughput sequencing offers an efficient and cost-effective approach to reduce genome complexity. Target enrichment typically yields higher read-depth for selected genes and is therefore suitable for determination of sequence polymorphisms and enables multiplexing of samples. Target enrichment also provides a means to annotate specific gene families within the sequenced organisms without the requirements for gene models. Here we describe enrichment procedures for NB-LRR-type plant resistance genes that can, for example, be used to establish the NB-LRR gene complements of individual plants and to map resistances more rapidly using a bulked segregant analysis.
authors.affiliation: The Sainsbury Laboratory, Norwich Research Park, Norwich, NR4 7UH, UK.
authors.forename: Florian
authors.initials: F
authors.lastname: Jupe
authors.forename: Xinwei
authors.initials: X
authors.lastname: Chen
authors.forename: Walter
authors.initials: W
authors.lastname: Verweij
authors.forename: Kamel
authors.initials: K
authors.lastname: Witek
authors.forename: Jonathan D G
authors.initials: JD
authors.lastname: Jones
authors.forename: Ingo
authors.initials: I
authors.lastname: Hein
mesh_terms: D053938:DNA Fragmentation; D018744:DNA, Plant / Q000302:isolation & purification / Q000378:metabolism*; D060467:Disease Resistance / Q000235:genetics*; D017343:Genes, Plant; D015698:Genomic Library; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D018744:DNA, Plant