title: Detection of changes in the structure and distribution map of triacylglycerol in fatty liver model by MALDI-SpiralTOF.
abstract: Matrix-assisted laser desorption/ionisation spiral orbit-type time-of-flight mass spectrometry (MALDI-SpiralTOF) can analyse lipid profiles and characterise lipid structure. Imaging mass spectrometry (IMS) also provides distribution maps of selected m/z values. Here, we investigated triacylglycerol (TG) structure and distribution using these technologies to estimate mouse fatty liver. The distribution and intensity of the most intense mass spectrum ion was indicated by IMS at m/z 881.7 (52:2). Analysis using MS/MS showed a structural change between liver TG and dietary TG. These findings suggest that MALDI-SpiralTOF is a powerful tool for clinical screening and estimating fatty liver.
authors.affiliation: Department of Traumatology and Critical Care Medicine, National Defense Medical College, Saitama 359-8513, Japan.
authors.forename: Kahoko
authors.initials: K
authors.lastname: Nishikawa
authors.affiliation: JEOL Ltd., Akishima, Tokyo 196-8553, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Hashimoto
authors.affiliation: JEOL Ltd., Akishima, Tokyo 196-8553, Japan.
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Itoh
authors.affiliation: Department of Laboratory Medicine, National Defense Medical College, Saitama 359-8513, Japan.
authors.forename: Sadayuki
authors.initials: S
authors.lastname: Hiroi
authors.affiliation: JEOL Ltd., Akishima, Tokyo 196-8553, Japan.
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kusai
authors.affiliation: Japan Self Defense Forces Hospital Yokosuka, Kanagawa 237-0071, Japan.
authors.forename: Fumihiko
authors.initials: F
authors.lastname: Hirata
authors.affiliation: Department of Traumatology and Critical Care Medicine, National Defense Medical College, Saitama 359-8513, Japan.
authors.forename: Toshihisa
authors.initials: T
authors.lastname: Sakamoto
authors.affiliation: Department of Pathology, National Defense Medical College, Saitama 359-8513, Japan.
authors.forename: Keiichi
authors.initials: K
authors.lastname: Iwaya
keywords: CID, collision-induced dissociation; Fatty liver; HE, haematoxlylin and eosin; HF, high-fat diet; HF + Res, high-fat diet supplemented with 0.2% resveratrol; IMS, imaging mass spectrometry; Imaging mass spectrometry; MALDI-SpiralTOF, matrix-assisted laser desorption/ionisation spiral orbit-type time-of-flight mass spectrometry; Matrix-assisted laser desorption/ionisation; Spiral orbit-type time-of-flight mass spectrometer; TG, triacylglycerol; TOF/TOF, tandem time-of-flight; Triacylglycerol