title: Effects of fatty acids on benzo[a]pyrene uptake and metabolism in human lung adenocarcinoma A549 cells.
abstract: Dietary supplementation with natural chemoprotective agents is receiving considerable attention because of health benefits and lack of toxicity. In recent in vivo and in vitro experimental studies, diets rich in n-3 polyunsaturated fatty acids have been shown to provide significant anti-tumor action. In this investigation, the effects of control fatty acids (oleic acid (OA), linoleic acid (LA)) and n-3 PUFA, e.g., docosahexaenoic acid (DHA) on the uptake and metabolism of the carcinogenic polycyclic aromatic hydrocarbon, benzo[a]pyrene (BaP) was investigated in A549 cells, a human adenocarcinoma alveolar basal epithelial cell line. A549 cells activate BaP through the cytochrome P450 enzyme system to form reactive metabolites, a few of which covalently bind to DNA and proteins. Therefore, multiphoton microscopy spectral analysis combined with linear unmixing was used to identify the parent compound and BaP metabolites formed in cells, in the presence and absence of fatty acids. The relative abundance of select metabolites was associated with altered P450 activity as determined using ethoxyresorufin-O-deethylase activity in cells cultured in the presence of BSA-conjugated fatty acids. In addition, the parent compound within cellular membranes increases significantly in the presence of each of the fatty acids, with the greatest accumulation observed following DHA treatment. DHA treated cells exhibit significantly lower pyrene-like metabolites indicative of lower adducts including DNA adducts compared to control BSA, OA or LA treated cells. Further, DHA reduced the abundance of the proximate carcinogen BaP 7,8-dihydrodiol and the 3-hydroxybenzo[a]pyrene metabolites compared to other treatments. The significant changes in BaP metabolites in DHA treated cells may be mediated by the effects on the physicochemical properties of the membrane known to affect enzyme activity related to phase I and phase II metabolism. In summary, DHA is a highly bioactive chemo-protective agent capable of modulating BaP-induced DNA adducts.
authors.affiliation: Department of Veterinary Integrative Biosciences, Texas A&M University, College Station, Texas, United States of America.
authors.forename: Rola
authors.initials: R
authors.lastname: Barhoumi
authors.affiliation: KAS CRSL, American University of Beirut, Beirut, Lebanon.
authors.forename: Youssef
authors.initials: Y
authors.lastname: Mouneimne
authors.affiliation: Department of Veterinary Integrative Biosciences, Texas A&M University, College Station, Texas, United States of America; Department of Nutrition and Food Science, Texas A&M University, College Station, Texas, United States of America.
authors.forename: Robert S
authors.initials: RS
authors.lastname: Chapkin
authors.affiliation: Department of Veterinary Integrative Biosciences, Texas A&M University, College Station, Texas, United States of America.
authors.forename: Robert C
authors.initials: RC
authors.lastname: Burghardt
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism*; D000077192:Adenocarcinoma of Lung; D000818:Animals; D001564:Benzo(a)pyrene / Q000378:metabolism*; D019328:Buthionine Sulfoximine / Q000494:pharmacology; D002417:Cattle; D045744:Cell Line, Tumor; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism; D018736:DNA Adducts / Q000378:metabolism; D004281:Docosahexaenoic Acids / Q000494:pharmacology; D005227:Fatty Acids / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism*; D017785:Photons; D012710:Serum Albumin, Bovine / Q000378:metabolism
chemical_list: D018736:DNA Adducts; D005227:Fatty Acids; C056612:benzo(a)pyrene-DNA adduct; D004281:Docosahexaenoic Acids; D012710:Serum Albumin, Bovine; D001564:Benzo(a)pyrene; D019328:Buthionine Sulfoximine; D019363:Cytochrome P-450 CYP1A1; D005978:Glutathione