title: Determination of melamine and cyromazine in milk by high performance liquid chromatography coupled with online solid-phase extraction using a novel cation-exchange restricted access material synthesized by surface initiated atom transfer radical polymerization.
abstract: A novel strong-cation-exchange restricted access material has been synthesized by atom transfer radical polymerization (ATRP). In the synthesis, poly(3-sulfopropyl methacrylate-co-ethylene dimethacrylate), [p(SPM/EDMA)] was grafted on the silica by surface-initiated ATRP first. The poly(glycerol mono-methacrylate) [pGMMA] was then immobilized on the external surface, which created a chemical diffusion barrier for protein exclusion. The resulting Sil-g-p(SPM/EDMA)-g-pGMMA has both functions of protein exclusion and cation exchange, exhibiting the property of cation-exchange restricted access material. The application of Sil-g-p(SPM/EDMA)-g-pGMMA has been studied by the determination of melamine and cyromazine in bovine milk using the online solid-phase extraction/HPLC method. In the process, the Sil-g-p(SPM/EDMA)-g-pGMMA was used for the sample pre-treatment and a HILIC column was employed as the analytical column. The method has shown good accuracy, precision and low limits of detections. The result demonstrated that the Sil-g-p(SPM/EDMA)-g-pGMMA can be used for the cation extraction from biological samples by direct HPLC injection.
authors.affiliation: College of Chemistry, Nankai University, Tianjin 300071, China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Chemistry, Nankai University, Tianjin 300071, China.
authors.forename: Shen
authors.initials: S
authors.lastname: Lin
authors.affiliation: College of Chemistry, Nankai University, Tianjin 300071, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Jiang
authors.affiliation: College of Chemistry, Nankai University, Tianjin 300071, China.
authors.forename: Xudong
authors.initials: X
authors.lastname: Zhu
authors.affiliation: College of Chemistry, Nankai University, Tianjin 300071, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Ling
authors.affiliation: College of Chemistry, Nankai University, Tianjin 300071, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Zhang
authors.affiliation: College of Chemistry, Nankai University, Tianjin 300071, China. Electronic address: xcdong@nankai.edu.cn.
authors.forename: Xiangchao
authors.initials: X
authors.lastname: Dong
mesh_terms: D000818:Animals; D002412:Cations; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D007475:Ion Exchange Resins / Q000138:chemical synthesis / Q000737:chemistry; D008689:Methacrylates / Q000737:chemistry; D008768:Methylmethacrylates / Q000737:chemistry; D008892:Milk / Q000737:chemistry*; D058105:Polymerization; D012822:Silicon Dioxide / Q000737:chemistry; D052616:Solid Phase Extraction / Q000379:methods; D014227:Triazines / Q000032:analysis*
chemical_list: C529206:3-sulfopropyl methacrylate; D002412:Cations; D007475:Ion Exchange Resins; D008689:Methacrylates; D008768:Methylmethacrylates; D014227:Triazines; C043583:glyceryl methyl methacrylate; D012822:Silicon Dioxide; C004919:ethylene dimethacrylate; C028704:cyromazine; C011907:melamine
keywords: Melamine and cyromazine determination; Milk sample; Online solid-phase extraction/HPLC analysis; Strong-cation-exchange restricted access material; Surface initiated atom transfer radical polymerization