title: Incorporation of adenine nucleotide transporter, Ant1p, into proteoliposomes facilitates ATP translocation and activation of encapsulated luciferase.
abstract: We prepared functional luciferase and membrane-integrated form of adenine nucleotide transporter (Ant1p) with a wheat germ cell-free system. The reconstituted Ant1p showed transport activity of ATP/AMP exchange across the membrane. Here we demonstrate that activity of the luciferase entrapped in the Ant1p-proteoliposomes is controllable by the external supply of ATP.
authors.affiliation: Proteo-Science Center, Ehime University, 3 Bunkyo-cho, Matsuyama, Ehime 790-8577, Japan. Electronic address: nozawa.akira.my@ehime-u.ac.jp.
authors.forename: Akira
authors.initials: A
authors.lastname: Nozawa
authors.affiliation: Proteo-Science Center, Ehime University, 3 Bunkyo-cho, Matsuyama, Ehime 790-8577, Japan. Electronic address: tozawa.yuzuru.mx.@ehime-u.ac.jp.
authors.forename: Yuzuru
authors.initials: Y
authors.lastname: Tozawa
mesh_terms: D000255:Adenosine Triphosphate / Q000737:chemistry*; D000818:Animals; D002474:Cell-Free System; D001517:Coleoptera; D004789:Enzyme Activation; D019476:Insect Proteins / Q000737:chemistry*; D008081:Liposomes; D008156:Luciferases / Q000737:chemistry*; D033704:Nucleotide Transport Proteins / Q000737:chemistry*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry*; D014908:Triticum / Q000737:chemistry
chemical_list: C430871:ANT1 protein, S cerevisiae; D019476:Insect Proteins; D008081:Liposomes; D033704:Nucleotide Transport Proteins; D029701:Saccharomyces cerevisiae Proteins; D000255:Adenosine Triphosphate; D008156:Luciferases
keywords: Artificial cell; Liposomes; Luciferase; Mitochondrial carrier; Peroxisome; Wheat germ cell-free system