title: Transient expressions of synthetic biology in plants.
abstract: Recent developments in transient expression methods have enabled the efficient delivery and expression of multiple genes within the same plant cell over a timescale of days. In some cases, the vectors deployed can be fine-tuned to allow differential expression of the various genes. This has opened the way to the deployment of transient expression for such applications as the production of macromolecular complexes and the analysis and manipulation of metabolic pathways. The ability to observe the effect of gene expression in a matter of days means that transient expression is becoming the method of choice for many plant-based synthetic biology applications.
authors.affiliation: The University of Queensland, Australian Institute for Bioengineering and Nanotechnology, Centre for Biomolecular Engineering, St Lucia, QLD 4072, Australia.
authors.forename: Frank
authors.initials: F
authors.lastname: Sainsbury
authors.affiliation: Department of Biological Chemistry, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, United Kingdom. Electronic address: george.lomonossoff@jic.ac.uk.
authors.forename: George P
authors.initials: GP
authors.lastname: Lomonossoff
mesh_terms: D017343:Genes, Plant; D018521:Plant Physiological Phenomena; D058615:Synthetic Biology