title: Synergistic antitumor activity of resveratrol and miR-200c in human lung cancer.
abstract: MicroRNAs have emerged as promising molecular factors with potential for clinical applications in cancer diagnosis and therapy. In the present study, we demonstrated that the level of miR-200c in lung cancer tissues was lower than that in normal tissues using real-time PCR. To further investigate the effects of miR-200c expression in lung cancer cells, we upregulated miR-200c levels in H460 cells using transfection. We found that the percentage of apoptotic cells was higher in the cells expressing miR-200c than that in the untransfected cells. Furthermore, the antitumor activities of miR-200c were demonstrated in vivo. Notably, we confirmed that reservatol (RESV) showed stronger antitumor activities in miR-200c-positive cells than in miR-200c-negative cells. Finally, we demonstrated that expression of miR-200c in H460 cells suppressed cell growth by targeting RECK, followed by activation of the JNK signaling pathway and ER stress. Collectively, these data show that miR-200c expression sensitizes H460 cells to RESV and this is likely due to RECK expression.
authors.affiliation: Department of Anaesthesiology, The First Hospital of China Medical University, Heping, Shenyang, Liaoning 110001, P.R. China.
authors.forename: Tao
authors.initials: T
authors.lastname: Bai
authors.affiliation: Department of Anaesthesiology, The First Hospital of China Medical University, Heping, Shenyang, Liaoning 110001, P.R. China.
authors.forename: Dao-Song
authors.initials: DS
authors.lastname: Dong
authors.affiliation: Department of Anaesthesiology, The First Hospital of China Medical University, Heping, Shenyang, Liaoning 110001, P.R. China.
authors.forename: Ling
authors.initials: L
authors.lastname: Pei
mesh_terms: D020533:Angiogenesis Inhibitors / Q000494:pharmacology; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D002289:Carcinoma, Non-Small-Cell Lung / Q000188:drug therapy* / Q000235:genetics / Q000401:mortality; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D059865:Endoplasmic Reticulum Stress; D058851:GPI-Linked Proteins / Q000096:biosynthesis*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D048031:JNK Mitogen-Activated Protein Kinases / Q000096:biosynthesis; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000401:mortality; D020935:MAP Kinase Signaling System; D051379:Mice; D008819:Mice, Nude; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics*; D009368:Neoplasm Transplantation; D000077185:Resveratrol; D012264:Ribonucleotide Reductases / Q000037:antagonists & inhibitors; D013267:Stilbenes / Q000494:pharmacology*; D014162:Transfection; D014183:Transplantation, Heterologous
chemical_list: D020533:Angiogenesis Inhibitors; D000972:Antineoplastic Agents, Phytogenic; D058851:GPI-Linked Proteins; C533896:MIRN200 microRNA, human; D035683:MicroRNAs; C115460:RECK protein, human; D013267:Stilbenes; D012264:Ribonucleotide Reductases; D048031:JNK Mitogen-Activated Protein Kinases; D000077185:Resveratrol