title: In vitro study of manganese-doped bioactive glasses for bone regeneration.
abstract: A glass belonging to the system SiO2-P2O5-CaO-MgO-Na2O-K2O was modified by introducing two different amounts of manganese oxide (MnO). Mn-doped glasses were prepared by melt and quenching technique and characterized by means of X-ray diffraction (XRD), scanning electron microscopy (SEM) observation and energy dispersion spectrometry (EDS) analysis. In vitro bioactivity test in simulated body fluid (SBF) showed a slight decrease in the reactivity kinetics of Mn-doped glasses compared to the glass used as control; however the glasses maintained a good degree of bioactivity. Mn-leaching test in SBF and minimum essential medium (MEM) revealed fluctuating trends probably due to a re-precipitation of Mn compounds during the bioactivity process. Cellular tests showed that all the Mn-doped glasses, up to a concentration of 50 μg/cm(2) (μg of glass powders/cm(2) of cell monolayer), did not produce cytotoxic effects on human MG-63 osteoblasts cultured for up to 5 days. Finally, biocompatibility tests demonstrated a good osteoblast proliferation and spreading on Mn-doped glasses and most of all that the Mn-doping can promote the expression of alkaline phosphatase (ALP) and some bone morphogenetic proteins (BMPs).
authors.affiliation: Applied Science and Technology Department, Politecnico di Torino, C.so Duca degli Abruzzi 24, 10129 Turin, Italy. Electronic address: marta.miola@polito.it.
authors.forename: Marta
authors.initials: M
authors.lastname: Miola
authors.affiliation: Applied Science and Technology Department, Politecnico di Torino, C.so Duca degli Abruzzi 24, 10129 Turin, Italy.
authors.forename: Chiara Vitale
authors.initials: CV
authors.lastname: Brovarone
authors.affiliation: Department of Clinical and Biological Sciences, University of Turin, Via Zuretti 29, 10126 Turin, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Maina
authors.affiliation: Department of Public Health and Pediatric Sciences, Piazza Polonia, 94, 10126 Torino, Italy.
authors.forename: Federica
authors.initials: F
authors.lastname: Rossi
authors.affiliation: Department of Oncology, University of Turin, Via Santena 5/bis, 10126 Turin, Italy.
authors.forename: Loredana
authors.initials: L
authors.lastname: Bergandi
authors.affiliation: Department of Oncology, University of Turin, Via Santena 5/bis, 10126 Turin, Italy.
authors.forename: Dario
authors.initials: D
authors.lastname: Ghigo
authors.affiliation: Department of Clinical and Biological Sciences, University of Turin, Corso Raffaello 30, 10125 Turin, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Saracino
authors.affiliation: Department of Clinical and Biological Sciences, University of Turin, Corso Raffaello 30, 10125 Turin, Italy.
authors.forename: Marina
authors.initials: M
authors.lastname: Maggiora
authors.affiliation: Department of Clinical and Biological Sciences, University of Turin, Corso Raffaello 30, 10125 Turin, Italy.
authors.forename: Rosa Angela
authors.initials: RA
authors.lastname: Canuto
authors.affiliation: Department of Clinical and Biological Sciences, University of Turin, Corso Raffaello 30, 10125 Turin, Italy.
authors.forename: Giuliana
authors.initials: G
authors.lastname: Muzio
authors.affiliation: Applied Science and Technology Department, Politecnico di Torino, C.so Duca degli Abruzzi 24, 10129 Turin, Italy.
authors.forename: Enrica
authors.initials: E
authors.lastname: Vernè
mesh_terms: D001861:Bone Regeneration / Q000187:drug effects*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D003460:Crystallization; D004055:Differential Thermal Analysis; D005786:Gene Expression Regulation / Q000187:drug effects; D005898:Glass / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D008345:Manganese / Q000494:pharmacology*; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D011208:Powders; D013052:Spectrometry, X-Ray Emission; D044366:Transition Temperature; D014961:X-Ray Diffraction
chemical_list: D011208:Powders; D008345:Manganese; D008315:Malondialdehyde; D007770:L-Lactate Dehydrogenase
keywords: Bioactivity; Cytotoxicity; Gene expression; Genetic design; Mn-doped glasses