title: Simultaneous determination of calycosin-7-O-β-d-glucoside, calycosin, formononetin, astragaloside IV and schisandrin in rat plasma by LC-MS/MS: application to a pharmacokinetic study after oral administration of Shenqi Wuwei chewable tablets.
abstract: A rapid, sensitive and reliable high-performance liquid chromatography-mass spectrometry (LC-MS/MS) method was developed and validated for simultaneous quantification of the five main bioactive components, calycosin, calycosin-7-O-β-d-glucoside, formononetin, astragaloside IV and schisandrin in rat plasma after oral administration of Shenqi Wuwei chewable tablets. Plasma samples were extracted using solid-phase extraction separated on a CEC18 column and detected by MS with an electrospray ionization interface in multiple-reaction monitoring mode. Calibration curves offered linear ranges of two orders of magnitude with r > 0.995. The method had a lower limit of quantitation of 0.1, 0.02, 0.1, 1 and 0.1 ng/mL for calycosin, calycosin-7-O-β-d-glucoside, formononetin, astragaloside IV and schisandrin, respectively. Intra- and inter-day precisions (relative standard deviation) for all analytes ranged from 0.97 to 7.63% and from 3.45 to 10.89%, respectively. This method was successfully applied to the pharmacokinetic study of the five compounds in rats after oral administration of Shenqi Wuwei chewable tablets.
authors.affiliation: Department of Pharmacy, General Hospital of Shenyang Military Area Command, No. 83, Wenhua Road, Shenhe District, Shenyang, 110840, People's Republic of China.
authors.forename: Xuehui
authors.initials: X
authors.lastname: Sun
authors.forename: Pingping
authors.initials: P
authors.lastname: Zhang
authors.forename: Xiujun
authors.initials: X
authors.lastname: Wu
authors.forename: Qiong
authors.initials: Q
authors.lastname: Wu
authors.forename: Mengmeng
authors.initials: M
authors.lastname: Zhang
authors.forename: Ye
authors.initials: Y
authors.lastname: An
authors.forename: Guobing
authors.initials: G
authors.lastname: Shi
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D034242:Cyclooctanes / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D004355:Drug Stability; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D005960:Glucosides / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D007529:Isoflavones / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D017705:Lignans / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D016014:Linear Models; D008297:Male; D011083:Polycyclic Compounds / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012503:Saponins / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D012680:Sensitivity and Specificity; D013607:Tablets; D053719:Tandem Mass Spectrometry / Q000379:methods; D014315:Triterpenes / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics
chemical_list: D034242:Cyclooctanes; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D007529:Isoflavones; D017705:Lignans; D011083:Polycyclic Compounds; D012503:Saponins; D013607:Tablets; D014315:Triterpenes; C526426:calycosin-7-O-beta-D-glucoside; C121707:7,3'-dihydroxy-4'-methoxyisoflavone; C007768:formononetin; C052064:astragaloside A; C011105:schizandrin
keywords: LC-MS/MS; Shenqi Wuwei chewable tablets; pharmacokinetic; rat plasma