title: Suppression of MicroRNA-203 improves survival of rat bone marrow mesenchymal stem cells through enhancing PI3K-induced cellular activation.
abstract: As a group of heterogeneous multipotent cells, mesenchymal stem cells (MSCs) have potential in treatment of a variety of clinical diseases. However, the low survival of the transplanted MSCs reduced their therapeutic effects. In this study, we revealed that rno-miR-203 suppressed activity and colony formation and enhanced apoptosis of the rat bone marrow-derived MSCs (BM-MSCs). Using bioinformatics analysis, we found a potential miR-203 binding site within rat phosphatidylinositol 3-kinase (PI3K) 3'UTR, and fluorescent reporter experiments validated the direct and negative regulation of PI3K expression by miR-203 through this site. Ectopic expression of PI3K rescued BM-MSCs from depressed activity induced by miR-203, and suppression of PI3K attenuated the increased BM-MSCs activity by miR-203 inhibitor treatment. Moreover, miR-203 blocking partly protected BM-MSCs from impairment caused by low nutrition. We conclude that inhibition of endogenous miR-203 elevated PI3K expression, which may strengthen PI3K/Akt pathway and promote BM-MSCs activity and survival. © 2014 IUBMB Life, 66(3):220-227, 2014.
authors.affiliation: Key Laboratory for Critical Care Medicine of the Ministry of Health, Tianjin, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Liu
authors.affiliation: First Center Clinical College, Tianjin Medical University, Tianjin, China.
authors.forename: Nan-Nan
authors.initials: NN
authors.lastname: Fu
authors.affiliation: The Organ Transplant Center, Tianjin First Center Hospital, Tianjin, China.
authors.forename: Hong-Li
authors.initials: HL
authors.lastname: Song
authors.affiliation: Key Laboratory for Critical Care Medicine of the Ministry of Health, Tianjin, China.
authors.forename: Yu-Liang
authors.initials: YL
authors.lastname: Wang
authors.affiliation: First Center Clinical College, Tianjin Medical University, Tianjin, China.
authors.forename: Ben-Juan
authors.initials: BJ
authors.lastname: Wu
authors.affiliation: The Organ Transplant Center, Tianjin First Center Hospital, Tianjin, China.
authors.forename: Zhong-Yang
authors.initials: ZY
authors.lastname: Shen
keywords: PI3K; mesenchymal stem cells; miR-203; microRNA; target gene