title: Dielectrophoresis-enhanced plasmonic sensing with gold nanohole arrays.
abstract: We experimentally demonstrate dielectrophoretic concentration of biological analytes on the surface of a gold nanohole array, which concurrently acts as a nanoplasmonic sensor and gradient force generator. The combination of nanohole-enhanced dielectrophoresis, electroosmosis, and extraordinary optical transmission through the periodic gold nanohole array enables real-time label-free detection of analyte molecules in a 5 μL droplet using concentrations as low as 1 pM within a few minutes, which is more than 1000 times faster than purely diffusion-based binding. The nanohole-based optofluidic platform demonstrated here is straightforward to construct, applicable to both charged and neutral molecules, and performs a novel function that cannot be accomplished using conventional surface plasmon resonance sensors.
authors.affiliation: Department of Electrical and Computer Engineering and ‡Department of Biomedical Engineering, University of Minnesota , Minneapolis, Minnesota 55455, United States.
authors.forename: Avijit
authors.initials: A
authors.lastname: Barik
authors.forename: Lauren M
authors.initials: LM
authors.lastname: Otto
authors.forename: Daehan
authors.initials: D
authors.lastname: Yoo
authors.forename: Jincy
authors.initials: J
authors.lastname: Jose
authors.forename: Timothy W
authors.initials: TW
authors.lastname: Johnson
authors.forename: Sang-Hyun
authors.initials: SH
authors.lastname: Oh
mesh_terms: D000818:Animals; D002417:Cattle; D053841:Electroosmosis / Q000295:instrumentation; D004586:Electrophoresis / Q000295:instrumentation; D004867:Equipment Design; D006046:Gold / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D012710:Serum Albumin, Bovine / Q000032:analysis* / Q000302:isolation & purification; D020349:Surface Plasmon Resonance / Q000295:instrumentation*
chemical_list: D012710:Serum Albumin, Bovine; D006046:Gold