title: Arachidonate 5-lipoxygenase-activating protein (ALOX5AP) gene rs4073259 polymorphism not associated with ischemic stroke in the northeastern Chinese Han population.
abstract: OBJECTIVE
  Although recent evidence has implicated that 5-lipoxygenase activating protein (ALOX5AP) gene is associated with ischemic stroke (IS) risk, the underlying molecular mechanism remains to be defined. This study aimed to investigate the role of ALOX5AP rs4073259 in ischemic stroke in a Northeastern Chinese Han population.
  
  
  METHODS
  A total of 501 IS patients and 497 healthy controls were enrolled for polymerase chain reaction (PCR) and ligase detection reaction (LDR) analysis of ALOX5AP rs4073259 single nucleotide polymorphism (SNP).
  
  
  RESULTS
  There were no statistically significant differences in ALOX5AP rs4073259 allele and genotype frequencies between IS or subtypes of IS and controls. There was no significant difference in genotype and allele frequencies of atherosclerosis degree between ischemic subjects with carotid artery plaque or absence. However, total cholesterol (TC), low-density lipoprotein (LDL), and high-density lipoprotein (HDL) in women patients were significantly higher than those in men (p=0.015, 0.000, and 0.008, respectively). Total homocysteine (tHcy) was higher in men patients than that in women (p=0.021).
  
  
  CONCLUSIONS
  There was no statistically significant association of ALOX5AP rs4073259 SNP with ischemic stroke in this northeastern Chinese Han population living in Heilongjiang province, China.
authors.affiliation: Department of Neurosurgery, The 2nd Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: RuYou
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Department of Ultrasound, The Fourth Hospital of Shijiazhuang, ShiJiaZhuang, China.
authors.forename: XiJuan
authors.initials: X
authors.lastname: Guo
authors.affiliation: Department of Ultrasound, The 2nd Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: XiaoYing
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Ultrasound, The 2nd Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Ultrasound, The 2nd Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: YanQing
authors.initials: Y
authors.lastname: Peng
authors.affiliation: Department of Ultrasound, The 2nd Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: XueSong
authors.initials: X
authors.lastname: Han
authors.affiliation: Department of Ultrasound, The 2nd Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: JiaWei
authors.initials: J
authors.lastname: Tian
authors.affiliation: Department of Ultrasound, The 2nd Affiliated Hospital of Harbin Medical University, Harbin, China. Electronic address: Litaosun1971@sina.com.
authors.forename: LiTao
authors.initials: L
authors.lastname: Sun
authors.affiliation: Department of Biostatistics, School of Public Health, Harbin Medical University, Harbin, China. Electronic address: liuyan@ems.hrbmu.edu.cn.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
mesh_terms: D058927:5-Lipoxygenase-Activating Proteins / Q000235:genetics*; D000328:Adult; D000368:Aged; D044466:Asian People / Q000235:genetics*; D002545:Brain Ischemia / Q000097:blood / Q000150:complications / Q000235:genetics*; D002340:Carotid Artery Diseases / Q000097:blood / Q000150:complications / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006710:Homocysteine / Q000097:blood; D006801:Humans; D008075:Lipoproteins, HDL / Q000097:blood; D008077:Lipoproteins, LDL / Q000097:blood; D008297:Male; D008875:Middle Aged; D058226:Plaque, Atherosclerotic / Q000097:blood / Q000150:complications / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D012720:Severity of Illness Index; D012737:Sex Factors; D020521:Stroke / Q000209:etiology / Q000235:genetics*
chemical_list: D058927:5-Lipoxygenase-Activating Proteins; C548592:ALOX5AP protein, human; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D006710:Homocysteine
keywords: Case and control study; Ischemic stroke; SNP