title: StRemorin1.3 hampers Potato virus X TGBp1 ability to increase plasmodesmata permeability, but does not interfere with its silencing suppressor activity.
abstract: The Triple Gene Block 1 (TGBp1) protein encoded by the Potato virus X is a multifunctional protein that acts as a suppressor of RNA silencing or facilitates the passage of virus from cell to cell by promoting the plasmodesmata opening. We previously showed that the membrane raft protein StRemorin1.3 is able to impair PVX infection. Here, we show that overexpressed StRemorin1.3 does not impair the silencing suppressor activity of TGBp1, but affects its ability to increase plasmodesmata permeability. A similar effect on plasmodesmata permeability was observed with other movement proteins, suggesting that REM is a general regulator of plasmodesmal size exclusion limit. These results add to our knowledge of the mechanisms underlying the StREM1.3 role in virus infection.
authors.affiliation: CNRS, Laboratoire de Biogenèse Membranaire (LBM), UMR 5200, F-33000 Villenave d'Ornon, France; Univ. Bordeaux, Laboratoire de Biogenèse Membranaire (LBM), UMR 5200, F-33000 Villenave d'Ornon, France.
authors.forename: Artemis
authors.initials: A
authors.lastname: Perraki
authors.affiliation: Laboratorio de Agrobiotecnología, DFBMC - Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, IBBEA, CONICET-UBA, Buenos Aires, Argentina.
authors.forename: Maria
authors.initials: M
authors.lastname: Binaghi
authors.affiliation: Laboratorio de Agrobiotecnología, DFBMC - Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, IBBEA, CONICET-UBA, Buenos Aires, Argentina.
authors.forename: Martin A
authors.initials: MA
authors.lastname: Mecchia
authors.affiliation: CNRS, Laboratoire de Biogenèse Membranaire (LBM), UMR 5200, F-33000 Villenave d'Ornon, France; Univ. Bordeaux, Laboratoire de Biogenèse Membranaire (LBM), UMR 5200, F-33000 Villenave d'Ornon, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Gronnier
authors.affiliation: Equipe de Virologie, UMR BFP 1332 INRA, CS 20032, 33882 Villenave d'Ornon, France.
authors.forename: Sylvie
authors.initials: S
authors.lastname: German-Retana
authors.affiliation: CNRS, Laboratoire de Biogenèse Membranaire (LBM), UMR 5200, F-33000 Villenave d'Ornon, France; Univ. Bordeaux, Laboratoire de Biogenèse Membranaire (LBM), UMR 5200, F-33000 Villenave d'Ornon, France. Electronic address: sebastien.mongrand@u-bordeaux2.fr.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Mongrand
authors.affiliation: CNRS, Laboratoire de Biogenèse Membranaire (LBM), UMR 5200, F-33000 Villenave d'Ornon, France; Univ. Bordeaux, Laboratoire de Biogenèse Membranaire (LBM), UMR 5200, F-33000 Villenave d'Ornon, France.
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Bayer
authors.affiliation: Laboratorio de Agrobiotecnología, DFBMC - Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, IBBEA, CONICET-UBA, Buenos Aires, Argentina.
authors.forename: Alicia M
authors.initials: AM
authors.lastname: Zelada
authors.affiliation: CNRS, Laboratoire de Biogenèse Membranaire (LBM), UMR 5200, F-33000 Villenave d'Ornon, France; Univ. Bordeaux, Laboratoire de Biogenèse Membranaire (LBM), UMR 5200, F-33000 Villenave d'Ornon, France.
authors.forename: Véronique
authors.initials: V
authors.lastname: Germain
mesh_terms: D060054:Agrobacterium / Q000235:genetics; D002352:Carrier Proteins / Q000502:physiology*; D015967:Gene Expression Regulation, Viral; D054884:Host-Pathogen Interactions; D008856:Microscopy, Fluorescence; D010539:Permeability; D010750:Phosphoproteins / Q000502:physiology*; D010940:Plant Proteins / Q000502:physiology*; D031425:Plasmodesmata / Q000378:metabolism* / Q000821:virology; D017863:Potexvirus / Q000502:physiology*; D020033:Protein Isoforms / Q000502:physiology; D021381:Protein Transport; D034622:RNA Interference; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D011198:Solanum tuberosum / Q000378:metabolism / Q000821:virology*; D014026:Nicotiana / Q000378:metabolism; D014764:Viral Proteins / Q000502:physiology*
chemical_list: D002352:Carrier Proteins; D010750:Phosphoproteins; D010940:Plant Proteins; D020033:Protein Isoforms; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; C103794:remorin
keywords: Plant membrane rafts; Plasmodesmata; Potato virus X; Potato virus Y; Remorin; Tobacco mosaic virus; Triple Gene Block protein 1; Viral suppressor of RNA gene silencing