title: Studies on voltammetric determination of cadmium in samples containing native and digested proteins.
abstract: This work focuses on determination of cadmium ions using anodic stripping voltammetry (ASV) on thin film mercury electrode in conditions corresponding to those obtained after digestion of cadmium-based quantum dots and their conjugates. It presents the impact of selected proteins, including potential receptors and surface blocking agents on the voltammetric determination of cadmium. Experiments regarding elimination of interferences related to proteins presence using sodium dodecyl sulfate (SDS) are also shown. Effect of SDS on selected analytical parameters and simplicity of analyses carried out was investigated in the framework of current studies. The significant differences of influence among tested proteins on ASV cadmium determination, as well as the variability in SDS effectiveness as the antifouling agent were observed and explained. This work is especially important for those, who design new bioassays and biosensors with a use of quantum dots as electrochemical labels, as it shows what problems may arise from presence of native and digested proteins in tested samples.
authors.affiliation: Department of Microbioanalytics, Institute of Biotechnology, Faculty of Chemistry, Warsaw University of Technology, Noakowskiego 3, 00-664 Warsaw, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Drozd
authors.affiliation: Department of Microbioanalytics, Institute of Biotechnology, Faculty of Chemistry, Warsaw University of Technology, Noakowskiego 3, 00-664 Warsaw, Poland. Electronic address: mariusz@ch.pw.edu.pl.
authors.forename: Mariusz
authors.initials: M
authors.lastname: Pietrzak
authors.affiliation: Department of Microbioanalytics, Institute of Biotechnology, Faculty of Chemistry, Warsaw University of Technology, Noakowskiego 3, 00-664 Warsaw, Poland.
authors.forename: Elżbieta
authors.initials: E
authors.lastname: Malinowska
mesh_terms: D002104:Cadmium / Q000032:analysis*; D055664:Electrochemical Techniques; D004566:Electrodes; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism*; D045663:Quantum Dots; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry
chemical_list: D011506:Proteins; D002104:Cadmium; D012967:Sodium Dodecyl Sulfate
keywords: Bioassays; Electrochemical biosensors; Labels; Quantum dots; Voltammetry