title: Bacterial metal resistance genes and metal bioavailability in contaminated sediments.
abstract: In bacteria a metal may be defined as bioavailable if it crosses the cytoplasmic membrane to reach the cytoplasm. Once inside the cell, specific metal resistance systems may be triggered. In this research, specific metal resistance genes were used to estimate metal bioavailability in sediment microbial communities. Gene levels were measured by quantitative PCR and correlated to metals in sediments using five different protocols to estimate dissolved, particle-adsorbed and occluded metals. The best correlations were obtained with czcA (a Cd/Zn/Co efflux pump) and Cd/Zn adsorbed or occluded in particles. Only adsorbed Co was correlated to czcA levels. We concluded that the measurement of czcA gene levels by quantitative PCR is a promising tool which may complement the classical approaches used to estimate Cd/Zn/Co bioavailability in sediment compartments.
authors.affiliation: Proteomics and Microbiology Lab, Research Institute for Biosciences, Université de Mons, 20 place du Parc, Avenue du Champ de Mars 6, B-7000 Mons, Belgium.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Roosa
authors.affiliation: Proteomics and Microbiology Lab, Research Institute for Biosciences, Université de Mons, 20 place du Parc, Avenue du Champ de Mars 6, B-7000 Mons, Belgium.
authors.forename: Ruddy
authors.initials: R
authors.lastname: Wattiez
authors.affiliation: Géosystèmes Lab, UFR de Chimie, Lille-1 University, Sciences and Technologies, 59655 Villeneuve d'Ascq, France.
authors.forename: Emilie
authors.initials: E
authors.lastname: Prygiel
authors.affiliation: Géosystèmes Lab, UFR de Chimie, Lille-1 University, Sciences and Technologies, 59655 Villeneuve d'Ascq, France.
authors.forename: Ludovic
authors.initials: L
authors.lastname: Lesven
authors.affiliation: Géosystèmes Lab, UFR de Chimie, Lille-1 University, Sciences and Technologies, 59655 Villeneuve d'Ascq, France.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Billon
authors.affiliation: Proteomics and Microbiology Lab, Research Institute for Biosciences, Université de Mons, 20 place du Parc, Avenue du Champ de Mars 6, B-7000 Mons, Belgium. Electronic address: david.gillan@umons.ac.be.
authors.forename: David C
authors.initials: DC
authors.lastname: Gillan
mesh_terms: D000327:Adsorption; D001419:Bacteria / Q000235:genetics*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D005798:Genes, Bacterial; D019015:Geologic Sediments / Q000737:chemistry*; D019216:Metals, Heavy / Q000032:analysis* / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000378:metabolism
chemical_list: C503087:CzcD protein, Bacillus subtilis; D019216:Metals, Heavy; D012097:Repressor Proteins; D014874:Water Pollutants, Chemical
keywords: Bacterial; Bioavailability; Metals; Resistance; Sediments