title: Evaluation of 3M molecular detection assay (MDA) Salmonella for the detection of Salmonella in selected foods: collaborative study.
abstract: The 3M Molecular Detection Assay (MDA) Salmonella is used with the 3M Molecular Detection System for the detection of Salmonella spp. in food, food-related, and environmental samples after enrichment. The assay utilizes loop-mediated isothermal amplification to rapidly amplify Salmonella target DNA with high specificity and sensitivity, combined with bioluminescence to detect the amplification. The 3M MDA Salmonella method was compared using an unpaired study design in a multilaboratory collaborative study to the U.S. Department of Agriculture/Food Safety and Inspection Service-Microbiology Laboratory Guidebook (USDA/FSIS-MLG 4.05), Isolation and Identification of Salmonella from Meat, Poultry, Pasteurized Egg and Catfish Products for raw ground beef and the U.S. Food and Drug Administration/Bacteriological Analytical Manual (FDA/BAM) Chapter 5 Salmonella reference method for wet dog food following the current AOAC guidelines. A total of 20 laboratories participated. For the 3M MDA Salmonella method, raw ground beef was analyzed using 25 g test portions, and wet dog food was analyzed using 375 g test portions. For the reference methods, 25 g test portions of each matrix were analyzed. Each matrix was artificially contaminated with Salmonella at three inoculation levels: an uninoculated control level (0 CFU/test portion), a low inoculum level (0.2-2 CFU/test portion), and a high inoculum level (2-5 CFU/test portion). In this study, 1512 unpaired replicate samples were analyzed. Statistical analysis was conducted according to the probability of detection (POD). For the low-level raw ground beef test portions, the following dLPOD (difference between the POD of the reference and candidate method) values with 95% confidence intervals were obtained: -0.01 (-0.14, +0.12). For the low-level wet dog food test portions, the following dLPOD with 95% confidence intervals were obtained: -0.04 (-0.16, +0.09). No significant differences were observed in the number of positive samples detected by the 3M MDA Salmonella method versus either the USDA/FSIS-MLG or FDA/BAM methods.
authors.forename: Patrick
authors.initials: P
authors.lastname: Bird
authors.forename: Kiel
authors.initials: K
authors.lastname: Fisher
authors.forename: Megan
authors.initials: M
authors.lastname: Boyle
authors.forename: Travis
authors.initials: T
authors.lastname: Huffman
authors.forename: M Joseph
authors.initials: MJ
authors.lastname: Benzinger
authors.forename: Paige
authors.initials: P
authors.lastname: Bedinghaus
authors.forename: Jonathan
authors.initials: J
authors.lastname: Flannery
authors.forename: Erin
authors.initials: E
authors.lastname: Crowley
authors.forename: James
authors.initials: J
authors.lastname: Agin
authors.forename: David
authors.initials: D
authors.lastname: Goins
authors.forename: DeAnn
authors.initials: D
authors.lastname: Benesh
authors.forename: John
authors.initials: J
authors.lastname: David
mesh_terms: D000465:Algorithms; D000821:Animal Feed / Q000382:microbiology*; D000818:Animals; D002417:Cattle; D004269:DNA, Bacterial / Q000032:analysis; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology / Q000592:standards*; D059022:Food Safety; D008460:Meat / Q000032:analysis* / Q000382:microbiology; D010063:Ovum / Q000382:microbiology; D011200:Poultry / Q000382:microbiology; D012015:Reference Standards; D015203:Reproducibility of Results; D012475:Salmonella / Q000302:isolation & purification*; D013234:Stem Cells; D014481:United States; D016885:United States Department of Agriculture
chemical_list: D004269:DNA, Bacterial