title: Substrate independent silver nanoparticle based antibacterial coatings.
abstract: Infections arising from bacterial adhesion and colonization on medical device surfaces are a significant healthcare problem. Silver based antibacterial coatings have attracted a great deal of attention as a potential solution. This paper reports on the development of a silver nanoparticles based antibacterial surface that can be applied to any type of material surface. The silver nanoparticles were surface engineered with a monolayer of 2-mercaptosuccinic acid, which facilitates the immobilization of the nanoparticles to the solid surface, and also reduces the rate of oxidation of the nanoparticles, extending the lifetime of the coatings. The coatings had excellent antibacterial efficacy against three clinically significant pathogenic bacteria i.e. Staphylococcus epidermidis, Staphylococcus aureus and Pseudomonas aeruginosa. Studies with primary human fibroblast cells showed that the coatings had no cytotoxicity in vitro. Innate immune studies in cultures of primary macrophages demonstrated that the coatings do not significantly alter the level of expression of pro-inflammatory cytokines or the adhesion and viability of these cells. Collectively, these coatings have an optimal combination of properties that make them attractive for deposition on medical device surfaces such as wound dressings, catheters and implants.
authors.affiliation: School of Engineering, University of South Australia, Mawson Lakes, SA 5095, Australia.
authors.forename: Shima
authors.initials: S
authors.lastname: Taheri
authors.affiliation: School of Engineering, University of South Australia, Mawson Lakes, SA 5095, Australia.
authors.forename: Alex
authors.initials: A
authors.lastname: Cavallaro
authors.affiliation: Sansom Institute, University of South Australia, Adelaide, SA 5000, Australia.
authors.forename: Susan N
authors.initials: SN
authors.lastname: Christo
authors.affiliation: Mawson Institute, University of South Australia, Mawson Lakes, SA 5095, Australia.
authors.forename: Louise E
authors.initials: LE
authors.lastname: Smith
authors.affiliation: School of Engineering, University of South Australia, Mawson Lakes, SA 5095, Australia.
authors.forename: Peter
authors.initials: P
authors.lastname: Majewski
authors.affiliation: School of Pharmacy and Medical Sciences, University of South Australia, SA 5000, Australia.
authors.forename: Mary
authors.initials: M
authors.lastname: Barton
authors.affiliation: School of Pharmacy and Medical Sciences, University of South Australia, SA 5000, Australia; Sansom Institute, University of South Australia, Adelaide, SA 5000, Australia.
authors.forename: John D
authors.initials: JD
authors.lastname: Hayball
authors.affiliation: School of Engineering, University of South Australia, Mawson Lakes, SA 5095, Australia; Mawson Institute, University of South Australia, Mawson Lakes, SA 5095, Australia. Electronic address: krasimir.vasilev@unisa.edu.au.
authors.forename: Krasimir
authors.initials: K
authors.lastname: Vasilev
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology*; D001424:Bacterial Infections / Q000188:drug therapy; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry* / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D051379:Mice; D008810:Mice, Inbred C57BL; D008826:Microbial Sensitivity Tests; D019736:Prostheses and Implants; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D012834:Silver / Q000737:chemistry* / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects; D013212:Staphylococcus epidermidis / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D020099:Coated Materials, Biocompatible; D012834:Silver
keywords: Antibacterial surfaces; Cytotoxicity; Infections; Innate inflammatory response; Medical devices; Silver nanoparticles