title: Indirect photometric detection of boron cluster anions electrophoretically separated in methanol.
abstract: 3,5-Dinitrobenzoate and picrate are light absorbing anions pertinent to indirect photometric detection of boron cluster anions in buffered methanolic background electrolytes (BGEs). Tris(hydroxymethyl)aminomethane and morpholine have been used as buffering bases, which eliminated baseline steps, and minimized the baseline noise. In methanolic BGEs, mobilities of boron cluster anions depend on both ionic constituents of the BGE buffer. This dependence can be explained by ion pair interaction of detected anions with BGE cations, which are not bonded into ion pairs with the BGE anions. The former ion pair interaction decreases sensitivity of the indirect photometric detection.
authors.affiliation: Institute of Biophysics, Academy of Sciences of the Czech Republic, v.v.i., Královopolská 135, 612 35 Brno, Czech Republic.
authors.forename: Lada
authors.initials: L
authors.lastname: Vítová
authors.affiliation: Institute of Biophysics, Academy of Sciences of the Czech Republic, v.v.i., Královopolská 135, 612 35 Brno, Czech Republic.
authors.forename: Lukáš
authors.initials: L
authors.lastname: Fojt
authors.affiliation: Institute of Biophysics, Academy of Sciences of the Czech Republic, v.v.i., Královopolská 135, 612 35 Brno, Czech Republic. Electronic address: vespalec@ibp.cz.
authors.forename: Radim
authors.initials: R
authors.lastname: Vespalec
mesh_terms: D000838:Anions / Q000737:chemistry; D001895:Boron / Q000737:chemistry*; D004573:Electrolytes; D019075:Electrophoresis, Capillary / Q000379:methods*; D000432:Methanol / Q000737:chemistry; D010783:Photometry / Q000379:methods*; D014325:Tromethamine / Q000737:chemistry
chemical_list: D000838:Anions; D004573:Electrolytes; D014325:Tromethamine; D001895:Boron; D000432:Methanol
keywords: Boron cluster; Capillary electrophoresis; Indirect photometry; Ion pair; Methanol; Mobility