title: Pharmacological evaluation and chemical standardization of an ayurvedic formulation for wound healing activity.
abstract: Wound healing is a topic of substantial prominence in Ayurveda, the Indian traditional system of medicine. Test drug Kshatantak Malam (KM), otherwise named as Baharer Nani, is described in Ayurveda since a long time for wound healing activity but necessitates scientific base. The test drug was prepared in the form of natural ointment with the plants like Achyranthes aspera, Allium cepa, and Canabis sativa under the base of butter in a specialized form of preparation. Chemical standardization was made on the basis of the physical character, rancidity test, extractive value, thin-layer chromatography, and gas chromatography. An 8-mm-diameter full-thickness punch was produced in Wistar rats. The test drug was applied topically and compared with standard comparators like framycetin ointment and povidone iodine ointment. Effects were observed on the basis of physical parameters like wound contraction size (mm(2)), wound index, healing period (days), tensile strength (g) and biochemical parameters like tissue DNA (mg/g), RNA (mg/g), total protein (mg/g), hydroxyproline (mg/g), PAGE study, and histopathological observations. Significant results (P < .05) were observed with KM in the punch wound model on the basis of various physical, biochemical, and histopathological parameters. The drug was found to be safe in acute and chronic toxicity models in animals. Chemically it is enriched with fatty substances.
authors.affiliation: 1The West Bengal University of Health Sciences, Kolkata, India.
authors.forename: Karuna Sagar
authors.initials: KS
authors.lastname: Gangopadhyay
authors.forename: Madhuchanda
authors.initials: M
authors.lastname: Khan
authors.forename: Srikanta
authors.initials: S
authors.lastname: Pandit
authors.forename: Shrabana
authors.initials: S
authors.lastname: Chakrabarti
authors.forename: Tapan Kumar
authors.initials: TK
authors.lastname: Mondal
authors.forename: Tuhin Kanti
authors.initials: TK
authors.lastname: Biswas
mesh_terms: D029044:Achyranthes; D000287:Administration, Topical; D000818:Animals; D002855:Chromatography, Thin Layer; D004195:Disease Models, Animal; D009824:Ointments; D008517:Phytotherapy / Q000379:methods*; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000187:drug effects / Q000293:injuries* / Q000473:pathology; D014945:Wound Healing / Q000187:drug effects*; D014947:Wounds and Injuries / Q000188:drug therapy* / Q000473:pathology
chemical_list: D009824:Ointments; D010936:Plant Extracts
keywords: chemical standardization; kshatantak malam; pharmacological screening; wound healing