title: Clinical predictive value ofin vitroanticancer drug sensitivity test for the therapeutic effect of adjuvant chemotherapy in patients with stage II-III colorectal cancer.
abstract: Clinically useful predictors of the efficacy of adjuvant chemotherapy following curative colorectal surgery remain to be determined. In the present study, we investigated the clinical utility of the collagen gel droplet-embedded culture drug sensitivity test (CD-DST) as a predictor of the therapeutic response to 5-fluorouracil (5-FU)-based adjuvant chemo-therapy in patients with stage II-III colorectal cancer. CD-DST was conducted using tumor samples surgically obtained from 189 patients. The therapeutic effect of 5-FU-based regimens between high (high-group) and low (low-group) sensitivity groups and a group that did not receive chemotherapy [CTx(-) group] was compared. CD-DST was successfully performed in 151 out of the 189 patients (79.9%), 87 of whom received 5-FU-based adjuvant chemotherapy after surgery. Twenty-seven of these 87 patients (31.0%) were classified as the high-group and the remaining 60 (69.0%) as the low-group. The 5-year recurrence-free survival (RFS) in the high-group was significantly higher compared to that in the low- and the CTx(-) groups. No differences in the 5-year RFS were observed between the low- and CTx(-) groups. In conclusion, CD-DST appears to be useful for predicting the therapeutic response to 5-FU-based adjuvant chemotherapy in patients with stage II-III colorectal cancer.
authors.affiliation: Department of Surgery, Shiga University of Medical Science, Otsu, Shiga 520-2192, Japan.
authors.forename: Eiji
authors.initials: E
authors.lastname: Mekata
authors.affiliation: Department of Surgery, Shiga University of Medical Science, Otsu, Shiga 520-2192, Japan.
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Sonoda
authors.affiliation: Department of Surgery, Shiga University of Medical Science, Otsu, Shiga 520-2192, Japan.
authors.forename: Tomoharu
authors.initials: T
authors.lastname: Shimizu
authors.affiliation: Department of Surgery, Shiga University of Medical Science, Otsu, Shiga 520-2192, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Tatsuta
authors.affiliation: Department of Surgery, Shiga University of Medical Science, Otsu, Shiga 520-2192, Japan.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Yamaguchi
authors.affiliation: Department of Surgery, Shiga University of Medical Science, Otsu, Shiga 520-2192, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Endo
authors.affiliation: Department of Surgery, Shiga University of Medical Science, Otsu, Shiga 520-2192, Japan.
authors.forename: Tohru
authors.initials: T
authors.lastname: Tani
keywords: adjuvant chemotherapy; collagen gel droplet-embedded culture drug sensitivity test; colorectal cancer