title: Identification of perfluorooctane sulfonate binding protein in the plasma of tiger pufferfish Takifugu rubripes.
abstract: It is well known that perfluorooctane sulfonate (PFOS) preferentially accumulates in the plasma of wildlife and humans. Although earlier studies have suggested that this was due to binding of PFOS to a plasma protein, definite characterization of the protein in in vivo exposure studies was not conducted thus far. In this study, we conducted both in vitro and in vivo experiments to identify PFOS binding protein in the plasma of fish. For the in vivo studies, PFOS was administered intraperitoneally to tiger pufferfish, Takifugu rubripes, and the plasma was separated by ammonium sulfate fractionation. High concentrations of PFOS were found in the 65-70 percent ammonium sulfate fraction (190ng/mL). After SDS-PAGE and N-terminal amino acid sequence analysis, the PFOS-binding protein was identified as an apolipoprotein A-I, which was confirmed on the basis of a significant correlation to the PFOS concentration in each fraction. The plasma samples fractionated by ammonium sulfate from untreated pufferfish were subjected to PFOS binding assay by the equilibrium dialysis method. The results further confirmed that the 60-65 percent ammonium sulfate fraction showed a high PFOS-binding ratio, similar to that found from in vivo studies. We demonstrated that PFOS is likely bound to an apolipoprotein A-I in the plasma of tiger pufferfish in in vivo and in vitro studies.
authors.affiliation: Laboratory of Marine Environmental Science, Faculty of Agriculture, Kyushu University, Hakozaki 6-10-1, Higashi-ku, Fukuoka 812-8581, Japan.
authors.forename: Masato
authors.initials: M
authors.lastname: Honda
authors.affiliation: Laboratory of Marine Environmental Science, Faculty of Agriculture, Kyushu University, Hakozaki 6-10-1, Higashi-ku, Fukuoka 812-8581, Japan.
authors.forename: Akemi
authors.initials: A
authors.lastname: Muta
authors.affiliation: Center for Advanced Instrumental and Educational Supports, Faculty of Agriculture, Kyushu University, Hakozaki 6-10-1, Higashi-ku, Fukuoka 812-8581, Japan.
authors.forename: Taiki
authors.initials: T
authors.lastname: Akasaka
authors.affiliation: Chemical Biotesting Center, Chemicals Evaluation and Research Institute, Bunkyo-ku, Tokyo 112-0004, Japan.
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Inoue
authors.affiliation: Laboratory of Marine Environmental Science, Faculty of Agriculture, Kyushu University, Hakozaki 6-10-1, Higashi-ku, Fukuoka 812-8581, Japan.
authors.forename: Yohei
authors.initials: Y
authors.lastname: Shimasaki
authors.affiliation: Wadsworth Center, New York State Department of Health, and School of Public Health, State University of New York at Albany, Albany, NY 12201-0509, USA.
authors.forename: Kurunthachalam
authors.initials: K
authors.lastname: Kannan
authors.affiliation: Laboratory of Marine Resource Chemistry, Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Hakozaki 6-10-1, Higashi-ku, Fukuoka 812-8581, Japan.
authors.forename: Nozomu
authors.initials: N
authors.lastname: Okino
authors.affiliation: Laboratory of Marine Environmental Science, Faculty of Agriculture, Kyushu University, Hakozaki 6-10-1, Higashi-ku, Fukuoka 812-8581, Japan. Electronic address: yoshima@agr.kyushu-u.ac.jp.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Oshima
mesh_terms: D017738:Alkanesulfonic Acids / Q000378:metabolism*; D000818:Animals; D016632:Apolipoprotein A-I / Q000097:blood / Q000378:metabolism; D001798:Blood Proteins / Q000737:chemistry / Q000378:metabolism; D002352:Carrier Proteins / Q000097:blood / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005466:Fluorocarbons / Q000378:metabolism*; D023741:Takifugu / Q000097:blood / Q000378:metabolism*
chemical_list: D017738:Alkanesulfonic Acids; D016632:Apolipoprotein A-I; D001798:Blood Proteins; D002352:Carrier Proteins; D005466:Fluorocarbons; C076994:perfluorooctane sulfonic acid
keywords: Apolipoprotein A–I; Binding protein; Perfluorooctane sulfonate; Plasma; Pufferfish