title: The use of cultured Drosophila cells for studying the microtubule cytoskeleton.
abstract: Cultured Drosophila cell lines have been developed into a powerful tool for studying a wide variety of cellular processes. Their ability to be easily and cheaply cultured as well as their susceptibility to protein knockdown via double-stranded RNA-mediated interference (RNAi) has made them the model system of choice for many researchers in the fields of cell biology and functional genomics. Here we describe basic techniques for gene knockdown, transgene expression, preparation for fluorescence microscopy, and centrosome enrichment using cultured Drosophila cells with an emphasis on studying the microtubule cytoskeleton.
authors.affiliation: Department of Cellular & Molecular Medicine, Arizona Cancer Center, Room 3951, University of Arizona, 1515 N. Campbell Avenue, Tucson, AZ, 85724, USA.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Nye
authors.forename: Daniel W
authors.initials: DW
authors.lastname: Buster
authors.forename: Gregory C
authors.initials: GC
authors.lastname: Rogers
mesh_terms: D000818:Animals; D002460:Cell Line; D018385:Centrosome / Q000378:metabolism; D003599:Cytoskeleton / Q000378:metabolism; D004330:Drosophila / Q000235:genetics / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D008870:Microtubules / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D059008:Time-Lapse Imaging