title: Anti-hepatitis B virus activity ofBoehmeria nivealeaf extracts in human HepG2.2.15 cells.
abstract: Boehmeria nivea(Linn.) Gaudich of the Urticaceae family is a perennial ratoon herbal plant, the root of which is used in traditional Chinese medicine and possesses a variety of pharmacological properties. The 20% ethanolBoehmeria nivearoot extract was shown to exert an anti-hepatitis B virus (HBV) effectin vitroandin vivo; however, whether theBoehmeria nivealeaf (BNL) extract possesses similar properties has not been determined. In this study, we aimed to investigate the anti-HBV effects of the BNL extract in HepG2.2.15 cells transfected with human HBV DNA. Our results demonstrated that the secretion of HBsAg and HBeAg was reduced in HepG2.2.15 cells treated with the BNL extract, without any recorded cytotoxic effects. In addition, the chloroform fraction (CF) and ethyl acetate fraction (EAF) of BNL were shown to be more potent compared to the other fractions: CF (100 mg/l) inhibited the secretion of HBsAg by 94.00±1.78% [inhibitory concentration 50 (IC50) = 20.92 mg/l] and that of HBeAg by 100.19±0.35% (IC50=19.67 mg/l) after 9 days of treatment. Similarly, EAF (200 mg/l) inhibited the secretion of HBsAg by 89.95±2.26% (IC50=39.90 mg/l) and that of HBeAg by 98.90±1.42% (IC50=36.45 mg/l). Furthermore, we observed that the content of HBV DNA in the medium secreted by the HepG2.2.15 cells was significantly decreased under CF (100 mg/l) or EAF (200 mg/l) treatment. Thus, we concluded that the BNL extracts exhibited anti-HBV activity, with CF and EAF being the most potent among the fractions.
authors.affiliation: Department of Pharmacology, Guilin Medical University, Guilin, Guangxi 541004, P.R. China.
authors.forename: Jingchen
authors.initials: J
authors.lastname: Wei
authors.affiliation: Laboratory of Hepatobiliary and Pancreatic Surgery, Affiliated Hospital of Guilin Medical University, Guilin, Guangxi 541001, P.R. China.
authors.forename: Lianku
authors.initials: L
authors.lastname: Lin
authors.affiliation: College of Resources and Environment, Guangxi Normal University, Guilin, Guangxi 541004, P.R. China.
authors.forename: Xiaojian
authors.initials: X
authors.lastname: Su
authors.affiliation: College of Resources and Environment, Guangxi Normal University, Guilin, Guangxi 541004, P.R. China.
authors.forename: Shaoyan
authors.initials: S
authors.lastname: Qin
authors.affiliation: Department of Pharmacology, Guilin Medical University, Guilin, Guangxi 541004, P.R. China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Xu
authors.affiliation: Department of Pharmacology, Guilin Medical University, Guilin, Guangxi 541004, P.R. China.
authors.forename: Zunian
authors.initials: Z
authors.lastname: Tang
authors.affiliation: Department of Pharmacology, Guilin Medical University, Guilin, Guangxi 541004, P.R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Deng
authors.affiliation: Department of Pharmacology, Guilin Medical University, Guilin, Guangxi 541004, P.R. China.
authors.forename: Yuehan
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Laboratory of Hepatobiliary and Pancreatic Surgery, Affiliated Hospital of Guilin Medical University, Guilin, Guangxi 541001, P.R. China ; Guangxi Key Laboratory of Molecular Medicine in Liver Injury and Repair, Affiliated Guilin Medical University, Guilin, Guangxi 541001, P.R. China.
authors.forename: Songqing
authors.initials: S
authors.lastname: He
keywords: Boehmeria nivea leaf; HBeAg; HBsAg; HepG2.2.15 cell; hepatitis B virus; hepatitis B virus DNA