title: Protein structure validation and analysis with X-ray crystallography.
abstract: X-ray crystallography is the main technique for the determination of protein structures. About 85 % of all protein structures known to date have been elucidated using X-ray crystallography. Knowledge of the three-dimensional structure of proteins can be used in various applications in biotechnology, biomedicine, drug design, and basic research and as a validation tool for protein modifications, ligand binding, and structural authenticity. Moreover, the requirement for pure, homogeneous, and stable protein solutions in crystallizations makes X-ray crystallography beneficial in other fields of protein research as well. Here, we describe the technique of X-ray protein crystallography and the steps involved for a successful three-dimensional crystal structure determination.
authors.affiliation: Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, BioCity, Tykistökatu 6, Turku, 20521, Finland, tassos.papageorgiou@btk.fi.
authors.forename: Anastassios C
authors.initials: AC
authors.lastname: Papageorgiou
authors.forename: Jesse
authors.initials: J
authors.lastname: Mattsson
mesh_terms: D018360:Crystallography, X-Ray / Q000379:methods*; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry*
chemical_list: D011506:Proteins