title: Effects of hydroformylation treatment on the storage time and blood group antigen expressions of reagent red blood cells.
abstract: OBJECTIVE
  To evaluate the effects of hydroformylation treatment on the storage time and blood group antigen expressions of reagent red blood cells (RBCs).
  
  
  METHODS
  RBCs from healthy donors were treated by using various final concentrations of paraformaldehyde (0.01%, 0.02%, 0.05%, 0.1%, 0.2%, 0.5% and 1.0%) and glutaraldehyde (0.01%, 0.025%, 0.05%, 0.1%, 0.2%, 0.5% and 1.0%), and one aliquot was used as control (untreated with aldehydes). Supernatant free hemoglobin (FHb) levels in all groups stored at 4 °C were detected every week, and the optimal procedure was selected. Expression of blood group antigens on RBCs treated by the optimal procedure was determined, and the total scores of blood group antigens were calculated.
  
  
  RESULTS
  0.2%, 0.5% and 1.0% Glutaraldehyde groups were ruled out directly due to serious crosslinking and aggregation of RBCs. As the extension of time, FHb levels in other 11 groups gradually increased (p<0.01 or p<0.05). FHb level in 0.025% glutaraldehyde group was significantly lower than that in other groups after 13 weeks (p<0.01), and the antigen strength of Fy(b), Jk(b), and Le(b) decreased slightly compared with those before treatment and storage (p<0.05), and there was no significant change for antigen strength of A, B, D, C, E, c, e, M, N, S, s, k, P1, Fy(a), Jk(a), and Le(a) (p>0.05).
  
  
  CONCLUSIONS
  0.025% Glutaraldehyde treatment can provide optimal protection for the membrane of RBCs and keep hemolysis at a low level after 13 weeks storage, and the majority of blood group antigen systems are not significantly affected, and the slight decline of Fy(b), Jk(b), and Le(b) antigen strength was acceptable for classical serological tests.
authors.affiliation: Department of Blood Transfusion, The Center for Clinical Transfusion Medicine, Chinese PLA General Hospital, Beijing, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Department of Blood Transfusion, The Center for Clinical Transfusion Medicine, Chinese PLA General Hospital, Beijing, China.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Sun
authors.affiliation: Department of Blood Transfusion, The Center for Clinical Transfusion Medicine, Chinese PLA General Hospital, Beijing, China.
authors.forename: Xiaozhen
authors.initials: X
authors.lastname: Guan
authors.affiliation: Department of Blood Transfusion, The Center for Clinical Transfusion Medicine, Chinese PLA General Hospital, Beijing, China.
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Blood Transfusion, The Center for Clinical Transfusion Medicine, Chinese PLA General Hospital, Beijing, China.
authors.forename: Chunya
authors.initials: C
authors.lastname: Ma
authors.affiliation: Department of Blood Transfusion, The Center for Clinical Transfusion Medicine, Chinese PLA General Hospital, Beijing, China.
authors.forename: Linfeng
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Blood Transfusion, The Center for Clinical Transfusion Medicine, Chinese PLA General Hospital, Beijing, China. Electronic address: deqingw@vip.sina.com.
authors.forename: Deqing
authors.initials: D
authors.lastname: Wang
mesh_terms: D001789:Blood Group Antigens / Q000096:biosynthesis*; D001793:Blood Preservation; D004912:Erythrocytes / Q000166:cytology / Q000378:metabolism*; D005260:Female; D005404:Fixatives / Q000494:pharmacology*; D005557:Formaldehyde / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D005976:Glutaral / Q000494:pharmacology*; D006801:Humans; D008297:Male; D011108:Polymers / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D001789:Blood Group Antigens; D005404:Fixatives; D011108:Polymers; D005557:Formaldehyde; D005976:Glutaral; C003043:paraform
keywords: Agglutination strength; Blood group antigen; Hemolysis; Hydroformylation; Reagent red blood cells