title: OCILRP2 signaling synergizes with LPS to induce the maturation and differentiation of murine dendritic cells.
abstract: Osteoclast Inhibitory Lectin-related Protein 2 (OCILRP2) is a typical type II transmembrane protein and belongs to C-type lectin-related protein family. It is preferentially expressed in dendritic cells (DC), B lymphocytes, and activated T lymphocytes. Upon binding to its ligand, OCILRP2 can promote CD28-mediated co-stimulation and enhance T cell activation. However, the role of OCILRP2 in DC development and activation is unclear. In this report, we present evidence that recombinant protein OCILRP2-Fc inhibits the generation and LPS-induced maturation of murine bone marrow-derived dendritic cells (BMDCs) by downregulating the expression of CD11c, MHC-II, and co-stimulators CD80 and CD86. OCILRP2-Fc also reduces the capacity of BMDCs to take up antigens, activates T cells, and secret inflammatory cytokines such as IL-6, IL-12, and TNF-α. Additionally, we show that OCILRP2-Fc may cause the aforementioned effects through inhibiting NF-κB activation. Therefore, OCILRP2 is a new regulator of DC maturation and differentiation following TLR4 activation.
authors.affiliation: Department of Microbiology and Immunology, Zhengzhou University School of Medicine, 100 Kexue Road, Zhengzhou 450001, People's Republic of China; Laboratory of Cellular and Molecular Immunology, Henan University School of Medicine, The New Campus, Jinming Road, Kaifeng 475004, People's Republic of China.
authors.forename: Lihui
authors.initials: L
authors.lastname: Chai
authors.affiliation: Laboratory of Cellular and Molecular Immunology, Henan University School of Medicine, The New Campus, Jinming Road, Kaifeng 475004, People's Republic of China.
authors.forename: Suxia
authors.initials: S
authors.lastname: Wu
authors.affiliation: Laboratory of Cellular and Molecular Immunology, Henan University School of Medicine, The New Campus, Jinming Road, Kaifeng 475004, People's Republic of China.
authors.forename: Guangchao
authors.initials: G
authors.lastname: Liu
authors.affiliation: Laboratory of Cellular and Molecular Immunology, Henan University School of Medicine, The New Campus, Jinming Road, Kaifeng 475004, People's Republic of China.
authors.forename: Zhanzheng
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Laboratory of Cellular and Molecular Immunology, Henan University School of Medicine, The New Campus, Jinming Road, Kaifeng 475004, People's Republic of China. Electronic address: wenzhi_tian@yahoo.com.
authors.forename: Wenzhi
authors.initials: W
authors.lastname: Tian
authors.affiliation: Laboratory of Cellular and Molecular Immunology, Henan University School of Medicine, The New Campus, Jinming Road, Kaifeng 475004, People's Republic of China. Electronic address: mayf@henu.edu.cn.
authors.forename: Yuanfang
authors.initials: Y
authors.lastname: Ma
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D016466:CHO Cells; D002454:Cell Differentiation; D002478:Cells, Cultured; D003412:Cricetulus; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology*; D006801:Humans; D037181:Lectins, C-Type / Q000276:immunology*; D008070:Lipopolysaccharides / Q000276:immunology*; D008565:Membrane Proteins / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D016328:NF-kappa B / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology; D015398:Signal Transduction
chemical_list: C470572:Dcl1 protein, mouse; D037181:Lectins, C-Type; D008070:Lipopolysaccharides; D008565:Membrane Proteins; D016328:NF-kappa B; D011994:Recombinant Proteins
keywords: C-type lectin; Dendritic cell; NF-κB; Osteoclast Inhibitory Lectin Related Protein 2 (OCILRP2); TLR