title: Protective ability of phenolics from white grape vinification by-products against structural damage of bovine serum albumin induced by glycation.
abstract: Grape skins recovered from white grape vinification processes were studied as possible anti-glycation agents. Total phenolics were characterised by the Folin Ciocalteu assay, proanthocyanidins by depolymerisation with n-butanol/HCl, flavonols by HPLC-DAD, reducing capacity by ferric ion reducing antioxidant power assay (FRAP) and anti-glycation activity by a bovine serum albumin (BSA)/fructose model system. Structural modifications of BSA were investigated by 2D isoelectric focusing sodium dodecyl sulfate polyacrylamide gel electrophoresis (IEF/SDS-PAGE) and fluorescence measurements. Both pI and Mr. of BSA were modified upon glycation reaction. These changes attributable to the involvement of free amino groups in Maillard-type reactions were inhibited by the white grape skin extracts. The anti-glycation activity ranged between 250 and 711mmol aminoguanidine Eq/kg. These results raise the interest in the potential health benefits of by-products of white grape vinification that could have a secondary use as an ingredient for new functional foods targeting wellbeing of diabetic and elderly people.
authors.affiliation: DeFENS, Department of Food, Environmental and Nutritional Sciences, Università degli Studi di Milano, via Celoria 2, 20133 Milano, Italy.
authors.forename: P S C
authors.initials: PS
authors.lastname: Sri Harsha
authors.affiliation: DeFENS, Department of Food, Environmental and Nutritional Sciences, Università degli Studi di Milano, via Celoria 2, 20133 Milano, Italy. Electronic address: vera.lavelli@unimi.it.
authors.forename: V
authors.initials: V
authors.lastname: Lavelli
authors.affiliation: DeFENS, Department of Food, Environmental and Nutritional Sciences, Università degli Studi di Milano, via Celoria 2, 20133 Milano, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Scarafoni
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D017127:Glycation End Products, Advanced / Q000737:chemistry*; D006801:Humans; D015416:Maillard Reaction; D010636:Phenols / Q000032:analysis*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D027843:Vitis / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D017127:Glycation End Products, Advanced; D010636:Phenols; D012710:Serum Albumin, Bovine
keywords: 2D-IEF/SDS–PAGE; Fluorescence; Phenolics; Protein glycation; White grape skins