title: Detection of anti-cyclic citrullinated peptide using a time-resolved fluoroimmunoassay.
abstract: In an effort to enhance the linear range of anti-CCP we developed a new immunoassay based on time-resolved fluoroimmunoassay. The precision, sensitivity, specificity, and stability of the assay were evaluated ELISA set as control. The anti-CCP IgG TRFIA kit we established had a wider detectable range than commercial ELISA ones. With regard to intra- and inter-assay precision, the TRFIA kit was better than threee commercial ELISA ones. The mean recovery rate was 101.0%. The TRFIA we developed for anti-CCP IgG detection yielded a more sensitive and reliable method for RA diagnosis and large-scale screening programs as well.
authors.affiliation: a The Third People's Hospital of Wuxi , Wuxi , China.
authors.forename: Jinhua
authors.initials: J
authors.lastname: Xiao
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Hu
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.forename: Mei
authors.initials: M
authors.lastname: Li
authors.forename: Yaohong
authors.initials: Y
authors.lastname: Zou
mesh_terms: D000818:Animals; D001172:Arthritis, Rheumatoid / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D001323:Autoantibodies / Q000097:blood*; D002138:Calibration; D004797:Enzyme-Linked Immunosorbent Assay; D005063:Europium / Q000737:chemistry; D015200:Fluoroimmunoassay / Q000379:methods* / Q000592:standards; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification; D051379:Mice; D010456:Peptides, Cyclic / Q000097:blood / Q000276:immunology*; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013194:Staining and Labeling / Q000379:methods
chemical_list: D001323:Autoantibodies; D007074:Immunoglobulin G; D010456:Peptides, Cyclic; D011933:Reagent Kits, Diagnostic; C487763:cyclic citrullinated peptide; D005063:Europium