title: Sensitivity enhancement in solution NMR: emerging ideas and new frontiers.
abstract: Modern NMR spectroscopy has reached an unprecedented level of sophistication in the determination of biomolecular structure and dynamics at atomic resolution in liquids. However, the sensitivity of this technique is still too low to solve a variety of cutting-edge biological problems in solution, especially those that involve viscous samples, very large biomolecules or aggregation-prone systems that need to be kept at low concentration. Despite the challenges, a variety of efforts have been carried out over the years to increase sensitivity of NMR spectroscopy in liquids. This review discusses basic concepts, recent developments and future opportunities in this exciting area of research.
authors.affiliation: Department of Chemistry and Biophysics Program, University of Wisconsin-Madison, 1101 University Avenue, Madison, WI 53706-1322, USA.
authors.forename: Jung Ho
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Chemistry and Biophysics Program, University of Wisconsin-Madison, 1101 University Avenue, Madison, WI 53706-1322, USA.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Okuno
authors.affiliation: Department of Chemistry and Biophysics Program, University of Wisconsin-Madison, 1101 University Avenue, Madison, WI 53706-1322, USA. Electronic address: cavagnero@chem.wisc.edu.
authors.forename: Silvia
authors.initials: S
authors.lastname: Cavagnero
mesh_terms: D000465:Algorithms; D000818:Animals; D006801:Humans; D006859:Hydrogen / Q000737:chemistry; D007202:Indicators and Reagents; D008958:Models, Molecular; D008968:Molecular Conformation; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D010777:Photochemistry; D013696:Temperature
chemical_list: D007202:Indicators and Reagents; D006859:Hydrogen
keywords: Dissolution DNP; Fast NMR; Hyperpolarization; NMR sensitivity; Overhauser DNP; PHIP; Photo-CIDNP; SEOP