title: Macrophages are required for dendritic cell uptake of respiratory syncytial virus from an infected epithelium.
abstract: We have previously shown that the respiratory syncytial virus [RSV] can productively infect monocyte derived dendritic cells [MoDC] and remain dormant within the same cells for prolonged periods. It is therefore possible that infected dendritic cells act as a reservoir within the airways of individuals between annual epidemics. In the present study we explored the possibility that sub-epithelial DCs can be infected with RSV from differentiated bronchial epithelium and that in turn RSV from DCs can infect the epithelium. A dual co-culture model was established in which a differentiated primary airway epithelium on an Air Liquid Interface (ALI) was cultured on a transwell insert and MoDCs were subsequently added to the basolateral membrane of the insert. Further experiments were undertaken using a triple co-culture model in which in which macrophages were added to the apical surface of the differentiated epithelium. A modified RSV [rr-RSV] expressing a red fluorescent protein marker of replication was used to infect either the MoDCs or the differentiated epithelium and infection of the reciprocal cell type was assessed using confocal microscopy. Our data shows that primary epithelium became infected when rr-RSV infected MoDCs were introduced onto the basal surface of the transwell insert. MoDCs located beneath the epithelium did not become infected with virus from infected epithelial cells in the dual co-culture model. However when macrophages were present on the apical surface of the primary epithelium infection of the basal MoDCs occurred. Our data suggests that RSV infected dendritic cells readily transmit infection to epithelial cells even when they are located beneath the basal layer. However macrophages appear to be necessary for the transmission of infection from epithelial cells to basal dendritic cells.
authors.affiliation: Department of Respiratory Medicine, Sheffield Children's Hospital, Sheffield, United Kingdom.
authors.forename: Kelechi
authors.initials: K
authors.lastname: Ugonna
authors.affiliation: Academic Unit of Respiratory Medicine, Dept. of Infection and Immunity University of Sheffield, Sheffield, United Kingdom.
authors.forename: Colin D
authors.initials: CD
authors.lastname: Bingle
authors.affiliation: Academic Unit of Respiratory Medicine, Dept. of Infection and Immunity University of Sheffield, Sheffield, United Kingdom.
authors.forename: Karen
authors.initials: K
authors.lastname: Plant
authors.affiliation: Academic Unit of Respiratory Medicine, Dept. of Infection and Immunity University of Sheffield, Sheffield, United Kingdom.
authors.forename: Kirsty
authors.initials: K
authors.lastname: Wilson
authors.affiliation: School Of Paediatrics and Child Health, University of Western Australia, Princess Margaret Hospital, Subiaco, Western Australia.
authors.forename: Mark L
authors.initials: ML
authors.lastname: Everard
mesh_terms: D000328:Adult; D002454:Cell Differentiation; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000473:pathology / Q000821:virology*; D004847:Epithelial Cells / Q000473:pathology / Q000821:virology; D004848:Epithelium / Q000473:pathology / Q000821:virology*; D005453:Fluorescence; D006801:Humans; D008264:Macrophages / Q000473:pathology / Q000821:virology*; D009000:Monocytes / Q000473:pathology; D015203:Reproducibility of Results; D018357:Respiratory Syncytial Virus Infections / Q000473:pathology / Q000821:virology*; D012136:Respiratory Syncytial Viruses / Q000502:physiology*; D014779:Virus Replication