title: Purification, characterization and functional role of lectin from green tiger shrimp Penaeus semisulcatus.
abstract: The present study reports the purification and characterization of immune molecule lectin from the green tiger shrimp Penaeus semiculcatus, which involves the non-self-recognition mechanism to destroy the infectious pathogens that continuously threaten their survival. P. semisulcatus lectin (Ps-Lec) was purified by affinity chromatography with mannose coupled sepharose CL-4B column and showed the 37 and 118kDa subunits in SDS-PAGE. The surface morphology of purified Ps-Lec exhibits the crystalline and aggregated nature in Transmission electron microscopy (TEM) analysis. Functional analysis of purified Ps-Lec showed that the broad spectrum of bacterial agglutination activity against Gram-positive and Gram-negative bacteria. In addition, Ps-Lec had the ability to involve in the haemoagglutination activity, which agglutinates the several vertebrate erythrocytes tested, and the haemagglutination titres were observed under light microscopy. Interestingly, Ps-Lec also exhibits the fungal aggregation activity against Candida albicans, this is the first report regarding the β-glucan recognition ability of fungal aggregation of Ps-Lec. Moreover, phenoloxidase activity was triggered by the Ps-Lec when compared to control. The present study reveals that Ps-Lec is an important multifunctional humoral defence protein plays a significant role against the bacterial and fungal cells.
authors.affiliation: Crustacean Molecular Biology and Genomics Lab, Department of Animal Health and Management, Alagappa University, Karaikudi 630 004, Tamil Nadu, India.
authors.forename: Jeyachandran
authors.initials: J
authors.lastname: Sivakamavalli
authors.affiliation: Crustacean Molecular Biology and Genomics Lab, Department of Animal Health and Management, Alagappa University, Karaikudi 630 004, Tamil Nadu, India. Electronic address: vaseeharanb@gmail.com.
authors.forename: Baskaralingam
authors.initials: B
authors.lastname: Vaseeharan
mesh_terms: D000818:Animals; D002176:Candida albicans / Q000187:drug effects; D002846:Chromatography, Affinity; D006384:Hemagglutination; D037102:Lectins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D033561:Penaeidae / Q000737:chemistry
chemical_list: D037102:Lectins
keywords: Agglutination activity; Bacterial agglutination; Candida albicans; Lectin; P. semisulcatus; PO activity