title: Potential biomarker of metformin action.
abstract: Metformin is a first-line, anti-diabetic agent prescribed to over 150 million people worldwide. The main effect of metformin is to suppress glucose production in the liver; however, there is no reliable biomarker to assess the effectiveness of metformin administration. Our previous studies have shown that phosphorylation of CBP at S436 is important for the regulation of hepatic glucose production by metformin. In current study, we found that CBP could be phosphorylated in white blood cells (WBCs), and CBP phosphorylation in the liver and in WBCs of mice had a similar pattern of change during a fasting time course experiment. These data suggests that CBP phosphorylation in WBCs may be used as a biomarker of metformin action in the liver.
authors.affiliation: Division of MetabolismDivision of EndocrinologyDepartments of Pediatrics, Physiology and Medicine, Johns Hopkins University School of Medicine, 600 North Wolfe Street, Baltimore, Maryland 21287, USAKennedy Krieger InstituteBaltimore, Maryland 21287, USA heling@jhmi.edu fwondis1@jhmi.edu.
authors.forename: Ling
authors.initials: L
authors.lastname: He
authors.affiliation: Division of MetabolismDivision of EndocrinologyDepartments of Pediatrics, Physiology and Medicine, Johns Hopkins University School of Medicine, 600 North Wolfe Street, Baltimore, Maryland 21287, USAKennedy Krieger InstituteBaltimore, Maryland 21287, USA.
authors.forename: Shumei
authors.initials: S
authors.lastname: Meng
authors.affiliation: Division of MetabolismDivision of EndocrinologyDepartments of Pediatrics, Physiology and Medicine, Johns Hopkins University School of Medicine, 600 North Wolfe Street, Baltimore, Maryland 21287, USAKennedy Krieger InstituteBaltimore, Maryland 21287, USADivision of MetabolismDivision of EndocrinologyDepartments of Pediatrics, Physiology and Medicine, Johns Hopkins University School of Medicine, 600 North Wolfe Street, Baltimore, Maryland 21287, USAKennedy Krieger InstituteBaltimore, Maryland 21287, USA.
authors.forename: Emily L
authors.initials: EL
authors.lastname: Germain-Lee
authors.affiliation: Division of MetabolismDivision of EndocrinologyDepartments of Pediatrics, Physiology and Medicine, Johns Hopkins University School of Medicine, 600 North Wolfe Street, Baltimore, Maryland 21287, USAKennedy Krieger InstituteBaltimore, Maryland 21287, USA.
authors.forename: Sally
authors.initials: S
authors.lastname: Radovick
authors.affiliation: Division of MetabolismDivision of EndocrinologyDepartments of Pediatrics, Physiology and Medicine, Johns Hopkins University School of Medicine, 600 North Wolfe Street, Baltimore, Maryland 21287, USAKennedy Krieger InstituteBaltimore, Maryland 21287, USA heling@jhmi.edu fwondis1@jhmi.edu.
authors.forename: Fredric E
authors.initials: FE
authors.lastname: Wondisford
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D015415:Biomarkers / Q000378:metabolism*; D050882:CREB-Binding Protein / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D005215:Fasting / Q000097:blood; D005260:Female; D005943:Gluconeogenesis / Q000187:drug effects; D005947:Glucose / Q000378:metabolism; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D007004:Hypoglycemic Agents / Q000494:pharmacology; D015151:Immunoblotting; D007962:Leukocytes / Q000187:drug effects* / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008687:Metformin / Q000494:pharmacology*; D051379:Mice; D009154:Mutation; D009765:Obesity / Q000097:blood; D010766:Phosphorylation / Q000187:drug effects; D012694:Serine / Q000378:metabolism
chemical_list: D015415:Biomarkers; D007004:Hypoglycemic Agents; D012694:Serine; D008687:Metformin; D050882:CREB-Binding Protein; D005947:Glucose
keywords: diabetes; glucose metabolism; liver; metabolism