title: Enhanced enzymatic hydrolysis of bamboo (Dendrocalamus giganteus Munro) culm by hydrothermal pretreatment.
abstract: Bamboo was non-isothermally pretreated with hot water at 140-200°C for different times (10-120 min). The effects of pretreatment conditions on the degradation of carbohydrates, cellulose crystallinity, partial removal/relocation of lignin, morphologic change of the feedstock, and glucose yield during the enzymatic hydrolysis were investigated. The effective removal of amorphous cellulose and hemicelluloses led to the increase of crystalline index of the residues. In comparison with the raw material, the surface of the pretreated samples was irregular and numerous lignin droplets appeared on the cellulose bundle surface under the intense pretreatment conditions. The glucose conversion increased with the raise of pretreatment temperature and the prolongation of time, and the maximum conversion of 75.7% was achieved for the sample pretreated at 200°C for 120 min, whereas the untreated sample was only 15.7%. The result illustrated that hydrothermal pretreatment affected the composition of bamboo, and remarkably enhanced the enzymatic hydrolysis efficiency.
authors.affiliation: Northwest A&F University, Yangling 712100, China; Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing 100083, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Xiao
authors.affiliation: Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing 100083, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Bian
authors.affiliation: Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing 100083, China.
authors.forename: Ming-Fei
authors.initials: MF
authors.lastname: Li
authors.affiliation: Northwest A&F University, Yangling 712100, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Xu
authors.affiliation: Northwest A&F University, Yangling 712100, China. Electronic address: xiaobin2093@sohu.com.
authors.forename: Bin
authors.initials: B
authors.lastname: Xiao
authors.affiliation: Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing 100083, China; State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510640, China. Electronic address: rcsun3@bjfu.edu.cn.
authors.forename: Run-Cang
authors.initials: RC
authors.lastname: Sun
mesh_terms: D031723:Bambusa / Q000187:drug effects* / Q000648:ultrastructure; D001709:Biotechnology / Q000379:methods*; D050260:Carbohydrate Metabolism / Q000187:drug effects; D002480:Cellulase / Q000378:metabolism*; D003460:Crystallization; D005936:Glucans / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006868:Hydrolysis / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D018547:Plant Stems / Q000187:drug effects*; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D014867:Water / Q000494:pharmacology*; D014961:X-Ray Diffraction
chemical_list: D005936:Glucans; D014867:Water; D002480:Cellulase; D005947:Glucose
keywords: Bamboo; Bioethanol; Enzyme saccharification; Hydrothermal pretreatment; Lignocellulose