title: A practical guide for nuclear resonance vibrational spectroscopy (NRVS) of biochemical samples and model compounds.
abstract: Nuclear resonance vibrational spectroscopy (NRVS) has been used by physicists for many years. However, it is still a relatively new technique for bioinorganic users. This technique yields a vibrational spectrum for a specific element, which can be easily interpreted. Furthermore, isotopic labeling allows for site-specific experiments. In this chapter, we discuss how to access specific beamlines, what kind of equipment is used in NRVS, and how the sample should be prepared and the data collected and analyzed.
authors.affiliation: Department of Chemistry, University of California, 1 Shields Avenue, Davis, CA, 95616, USA.
authors.forename: Hongxin
authors.initials: H
authors.lastname: Wang
authors.forename: Esen Ercan
authors.initials: EE
authors.lastname: Alp
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Yoda
authors.forename: Stephen P
authors.initials: SP
authors.lastname: Cramer
mesh_terms: D002138:Calibration; D007503:Iron Isotopes; D009682:Magnetic Resonance Spectroscopy; D011506:Proteins / Q000032:analysis*; D013057:Spectrum Analysis / Q000379:methods*; D017356:Synchrotrons; D014732:Vibration
chemical_list: D007503:Iron Isotopes; D011506:Proteins