title: Humans and great apes cohabiting the forest ecosystem in central african republic harbour the same hookworms.
abstract: BACKGROUND
  Hookworms are important pathogens of humans. To date, Necator americanus is the sole, known species of the genus Necator infecting humans. In contrast, several Necator species have been described in African great apes and other primates. It has not yet been determined whether primate-originating Necator species are also parasitic in humans.
  
  
  RESULTS
  The infective larvae of Necator spp. were developed using modified Harada-Mori filter-paper cultures from faeces of humans and great apes inhabiting Dzanga-Sangha Protected Areas, Central African Republic. The first and second internal transcribed spacers (ITS-1 and ITS-2) of nuclear ribosomal DNA and partial cytochrome c oxidase subunit 1 (cox1) gene of mtDNA obtained from the hookworm larvae were sequenced and compared. Three sequence types (I-III) were recognized in the ITS region, and 34 cox1 haplotypes represented three phylogenetic groups (A-C). The combinations determined were I-A, II-B, II-C, III-B and III-C. Combination I-A, corresponding to N. americanus, was demonstrated in humans and western lowland gorillas; II-B and II-C were observed in humans, western lowland gorillas and chimpanzees; III-B and III-C were found only in humans. Pairwise nucleotide difference in the cox1 haplotypes between the groups was more than 8%, while the difference within each group was less than 2.1%.
  
  
  CONCLUSIONS
  The distinctness of ITS sequence variants and high number of pairwise nucleotide differences among cox1 variants indicate the possible presence of several species of Necator in both humans and great apes. We conclude that Necator hookworms are shared by humans and great apes co-habiting the same tropical forest ecosystems.
authors.affiliation: Department of Biology, Oita University, Yufu, Oita, Japan.
authors.forename: Hideo
authors.initials: H
authors.lastname: Hasegawa
authors.affiliation: Department of Pathology and Parasitology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic; Central European Institute of Technology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic; Biology Centre, Institute of Parasitology, Academy of Sciences of the Czech Republic, Ceske Budejovice, Czech Republic.
authors.forename: David
authors.initials: D
authors.lastname: Modrý
authors.affiliation: Department of Biology, Oita University, Yufu, Oita, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kitagawa
authors.affiliation: Department of Anthropology, Durham University, Durham, United Kingdom.
authors.forename: Kathryn A
authors.initials: KA
authors.lastname: Shutt
authors.affiliation: World Wildlife Foundation (WWF), Dzanga Sangha Protected Areas, Bangui, Central African Republic.
authors.forename: Angelique
authors.initials: A
authors.lastname: Todd
authors.affiliation: Department of Pathology and Parasitology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic.
authors.forename: Barbora
authors.initials: B
authors.lastname: Kalousová
authors.affiliation: Department of Pathology and Parasitology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic.
authors.forename: Ilona
authors.initials: I
authors.lastname: Profousová
authors.affiliation: Department of Pathology and Parasitology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic; Biology Centre, Institute of Parasitology, Academy of Sciences of the Czech Republic, Ceske Budejovice, Czech Republic; Liberec Zoo, Liberec, Czech Republic; Institute of Vertebrate Biology, Academy of Sciences of the Czech Republic, Brno, Czech Republic.
authors.forename: Klára J
authors.initials: KJ
authors.lastname: Petrželková
mesh_terms: D000818:Animals; D002488:Central African Republic / Q000453:epidemiology; D016000:Cluster Analysis; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D017753:Ecosystem; D003576:Electron Transport Complex IV / Q000235:genetics; D005838:Genotype; D006801:Humans; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D009331:Necator / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D009332:Necatoriasis / Q000453:epidemiology / Q000469:parasitology* / Q000662:veterinary*; D002679:Pan troglodytes; D010802:Phylogeny; D018419:Primate Diseases / Q000453:epidemiology / Q000469:parasitology; D011323:Primates; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014197:Trees
chemical_list: D021903:DNA, Ribosomal Spacer; D003576:Electron Transport Complex IV