title: Cytotoxicity and anti-inflammatory effects of root bark extracts of Acanthopanax henryi.
abstract: OBJECTIVE
  To investigate the cytotoxicity, anti-inflammatory activity, and action mechanism of root bark extracts of Acanthopanax henryi.
  
  
  METHODS
  The hot methanol extract of the root bark of A. henryi was subjected to XAD-4 column chromatography eluting with a gradient of methanol in water. The cytotoxicity and anti-inflammatory effects of the MeOH fractions were evaluated on the inhibition on lipopolysaccharide (LPS)-induced nitric oxide, prostaglandin E2, interleukin-1β, and interleukin-6 production in RAW 264.7 macrophages.
  
  
  RESULTS
  The 80% MeOH fraction was a better inhibitor of LPS-induced NO, PGE2, IL-1β, and IL-6 production, and expression of inducible nitric oxide synthase (iNOS) at the protein levels in a concentration-dependent manner.
  
  
  CONCLUSIONS
  The 80% MeOH fraction of A. henryi root bark has significant anti-inflammatory activity. This provides a pharmacological basis for clinical application for the treatment of inflammation.
authors.affiliation: Herbal Medicine Research Division, Korea Food & Drug Administration, Seoul 363-700, Korea.
authors.forename: Jong-Hwan
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Hunan Key Laboratory of Traditional Chinese Medicine modernization, School of Pharmacy, Hunan University of Chinese Medicine, Hunan Changsha 410208, China. Electronic address: lxq0001cn@163.com.
authors.forename: Xiang-Qian
authors.initials: XQ
authors.lastname: Liu
authors.affiliation: Hunan Key Laboratory of Traditional Chinese Medicine modernization, School of Pharmacy, Hunan University of Chinese Medicine, Hunan Changsha 410208, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Dai
authors.affiliation: College of Pharmacy, Kyung-Hee University, Seoul 130-701, Korea.
authors.forename: Chang-Soo
authors.initials: CS
authors.lastname: Yook
authors.affiliation: College of Pharmacy, Kyung-Hee University, Seoul 130-701, Korea.
authors.forename: Kyung-Tae
authors.initials: KT
authors.lastname: Lee
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology / Q000627:therapeutic use*; D015232:Dinoprostone / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D028001:Eleutherococcus; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D053583:Interleukin-1beta / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008070:Lipopolysaccharides; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D008517:Phytotherapy; D024301:Plant Bark; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D018517:Plant Roots
chemical_list: D000893:Anti-Inflammatory Agents; D053583:Interleukin-1beta; D015850:Interleukin-6; D008070:Lipopolysaccharides; D010936:Plant Extracts; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; D015232:Dinoprostone
keywords: Acanthopanax henryi (Oliv.) Harms; Anti-inflammatory activity; Cytotoxic activity; Root bark extract