title: Extraction of extracellular lipids from chemoautotrophic bacteria Serratia sp. ISTD04 for production of biodiesel.
abstract: A CO2 sequestering bacterial strain, Serratia sp. ISTD04, that produces a significant amount of extracellular lipids was isolated from marble mine rocks. (14)C labeling analysis revealed that the rate of assimilation of CO2 by the strain is 0.756×10(-9)μmolCO2fixedcell(-1)h(-1). It was found to produce 466mg/l of extracellular lipid which was characterized using (1)H NMR. After transesterification of lipids, the total saturated and unsaturated FAME was found to be 51% and 49% respectively. The major FAME contained in the biodiesel were palmitic acid methyl ester (C16:0), oleic acid methyl ester (C18:1) and 10-nonadecenoic acid methyl ester (C19:1). Biodiesel produced by Serratia sp. ISTD04 is balanced in terms of FAME composition of good quality. It also contained higher proportion of oleic acid (35%) which makes it suitable for utilization in existing engines. Thus, the strain can be harnessed commercially to sequester CO2 into biodiesel.
authors.affiliation: School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110 067, India.
authors.forename: Randhir K
authors.initials: RK
authors.lastname: Bharti
authors.affiliation: School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110 067, India.
authors.forename: Shaili
authors.initials: S
authors.lastname: Srivastava
authors.affiliation: School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110 067, India. Electronic address: isthakur@hotmail.com.
authors.forename: Indu Shekhar
authors.initials: IS
authors.lastname: Thakur
mesh_terms: D056804:Biofuels; D001709:Biotechnology / Q000379:methods*; D002245:Carbon Dioxide / Q000378:metabolism; D052837:Chemoautotrophic Growth; D004952:Esters / Q000378:metabolism; D005110:Extracellular Space / Q000737:chemistry; D008055:Lipids / Q000302:isolation & purification*; D012705:Serratia / Q000378:metabolism*
chemical_list: D056804:Biofuels; D004952:Esters; D008055:Lipids; D002245:Carbon Dioxide
keywords: Biodiesel; Extracellular lipids; Transesterification processes