title: Trigocherrierin A, a potent inhibitor of chikungunya virus replication.
abstract: Trigocherrierin A (1) and trigocherriolide E (2), two new daphnane diterpenoid orthoesters (DDOs), and six chlorinated analogues, trigocherrins A, B, F and trigocherriolides A-C, were isolated from the leaves of Trigonostemon cherrieri. Their structures were identified by mass spectrometry, extensive one- and two-dimensional NMR spectroscopy and through comparison with data reported in the literature. These compounds are potent and selective inhibitors of chikungunya virus (CHIKV) replication. Among the DDOs isolated, compound 1 exhibited the strongest anti-CHIKV activity (EC₅₀ = 0.6 ± 0.1 µM, SI = 71.7).
authors.affiliation: EA4267 Epithelial Functions and Dysfunctions, UFR of Medical and Pharmaceutical Sciences, 19 rue Ambroise Paré, 25030 Besançon, France. melanie.bourjot@univ-fcomte.fr.
authors.forename: Mélanie
authors.initials: M
authors.lastname: Bourjot
authors.affiliation: Rega Institute for Medical Research (KU Leuven), Minderbroedersstraat 10, B3000, Leuven, Belgium. Pieter.Leyssen@rega.kuleuven.be.
authors.forename: Pieter
authors.initials: P
authors.lastname: Leyssen
authors.affiliation: Rega Institute for Medical Research (KU Leuven), Minderbroedersstraat 10, B3000, Leuven, Belgium. Johan.Neyts@rega.kuleuven.be.
authors.forename: Johan
authors.initials: J
authors.lastname: Neyts
authors.affiliation: Gif Research Center, Institute of Chemistry of Natural Substances (ICSN), CNRS, Labex CEBA, 1, avenue de la Terrasse, 91198 Gif sur Yvette Cedex, France. vincent.dumontet@cnrs.fr.
authors.forename: Vincent
authors.initials: V
authors.lastname: Dumontet
authors.affiliation: Gif Research Center, Institute of Chemistry of Natural Substances (ICSN), CNRS, Labex CEBA, 1, avenue de la Terrasse, 91198 Gif sur Yvette Cedex, France. marc.litaudon@cnrs.fr.
authors.forename: Marc
authors.initials: M
authors.lastname: Litaudon
mesh_terms: D000998:Antiviral Agents / Q000737:chemistry / Q000494:pharmacology*; D002646:Chikungunya virus / Q000187:drug effects* / Q000502:physiology*; D004224:Diterpenes / Q000737:chemistry / Q000494:pharmacology*; D008958:Models, Molecular; D015394:Molecular Structure; D053147:Plant Exudates / Q000737:chemistry; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D000998:Antiviral Agents; D004224:Diterpenes; D053147:Plant Exudates; C571266:trigocherrin A