title: Biosensor based on lectin and lipid membranes for detection of serum glycoproteins in infected patients with dengue.
abstract: In this work, we developed a biosystem based on Concanavalin A (ConA) and lipid membranes to recognize glycoproteins from the serum of patients contaminated with dengue serotypes 1, 2 and 3 (DENV1, DENV2 and DENV3). The modified gold electrode was characterized using cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and atomic force microscopy. Morphological analyses of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), DPPC-ConA, DPPC-ConA-DENV1, DPPC-ConA-DENV2 and DPPC-ConA-DENV3 revealed the existence of a non-uniform covering and large globules. EIS and CV measurements have shown that redox probe reactions on the modified gold electrodes were partially blocked due to the adsorption of lipid-ConA system and reveal the interaction response of the immobilized ConA to the presence of glycoproteins of dengue serum. The biosystem exhibited a wide linear response to different concentrations of sera of dengue serotypes 1, 2 and 3. A higher impedimetric response to glycoproteins present in dengue serotype 3 was observed. Our results demonstrate the applicability of lectin and lipid membranes to the development of biosensors for dengue infections.
authors.affiliation: Programa de Pós-Graduação em Inovação Terapêutica, Universidade Federal de Pernambuco, 50670-901 Recife, PE, Brazil.
authors.forename: Débora M N
authors.initials: DM
authors.lastname: Luna
authors.affiliation: Departamento de Bioquímica, Universidade Federal de Pernambuco, 50670-901 Recife, PE, Brazil.
authors.forename: Maria D L
authors.initials: MD
authors.lastname: Oliveira
authors.affiliation: Departamento de Doenças Infecciosas e Parasitárias, FAMERP, 15090-000 São José do Rio Preto, SP, Brazil.
authors.forename: Maurício L
authors.initials: ML
authors.lastname: Nogueira
authors.affiliation: Programa de Pós-Graduação em Inovação Terapêutica, Universidade Federal de Pernambuco, 50670-901 Recife, PE, Brazil; Departamento de Bioquímica, Universidade Federal de Pernambuco, 50670-901 Recife, PE, Brazil. Electronic address: csrandrade@gmail.com.
authors.forename: Cesar A S
authors.initials: CA
authors.lastname: Andrade
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine / Q000378:metabolism*; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002462:Cell Membrane; D003208:Concanavalin A / Q000378:metabolism*; D003715:Dengue / Q000097:blood* / Q000175:diagnosis; D004563:Electrochemistry; D004566:Electrodes; D006023:Glycoproteins / Q000097:blood*; D006046:Gold / Q000737:chemistry; D006801:Humans; D008081:Liposomes / Q000378:metabolism; D019095:Point-of-Care Systems
chemical_list: D006023:Glycoproteins; D008081:Liposomes; D003208:Concanavalin A; D015060:1,2-Dipalmitoylphosphatidylcholine; D006046:Gold
keywords: Biosensor; Dengue; Glycoproteins; Lectin; Phospholipid