title: Proteomic analysis of the follicular fluid of Tianzhu white yak during diestrus.
abstract: The aim of this study was to identify differentially expressed proteins in the follicular fluid of Tianzhu white yak during diestrus. Follicles obtained from female yak were divided into four groups according to their diameter: 0-2, 2-4, 4-6 mm, and greater than 6 mm. The follicular fluid was directly aspirated from the follicles and mixed according to follicular size, and two-dimensional gel electrophoresis was carried out on the crude follicular fluid samples. Thirty-four differentially expressed spots were generated from these four sizes of follicles. Fourteen of these spots were analyzed by MALDI-TOF/TOF-MS and identified as: AS3MT, VDP, ANKRD6, C10orf107 protein, MRP4, MAPKAP1, AGO3, profilin-β-actin, SPT2 homolog, AGP, AR, RNF20, obscurin-like-1, and one unnamed protein. These proteins were first reported in follicular fluid, in addition to VDP and AGP. Based on existing knowledge of their function and patterns of expression, we hypothesize that most of these differentially expressed proteins play a role in ovarian follicular growth and development, dominant follicle selection, or follicular atresia and development of oocytes; however, the function of the other differentially expressed proteins in reproduction remains ambiguous.
authors.affiliation: Agricultural College, Ningxia University, Yinchuan 750021, China. tao_jz@nxu.edu.cn.
authors.forename: Jinzhong
authors.initials: J
authors.lastname: Tao
authors.affiliation: College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China. zhaogs111@163.com.
authors.forename: Guoshun
authors.initials: G
authors.lastname: Zhao
authors.affiliation: College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China. zhaoxx@gsau.edu.cn.
authors.forename: Xingxu
authors.initials: X
authors.lastname: Zhao
authors.affiliation: College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China. lifd@gsau.edu.cn.
authors.forename: Fadi
authors.initials: F
authors.lastname: Li
authors.affiliation: College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China. wx.258.h@st.gsau.edu.cn.
authors.forename: Xiaohu
authors.initials: X
authors.lastname: Wu
authors.affiliation: College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China. hujj@gsau.edu.cn.
authors.forename: Junjie
authors.initials: J
authors.lastname: Hu
authors.affiliation: College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China. zhychy@163.com.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002417:Cattle; D004031:Diestrus / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D015571:Follicular Fluid / Q000378:metabolism*; D006080:Ovarian Follicle / Q000378:metabolism; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D020543:Proteome