title: Proteomic analyses of genes regulated by heterogeneous nuclear ribonucleoproteins A/B in Jurkat cells.
abstract: Several lines of evidence suggest that hnRNPs A/B (hnRNPs A1, A2/B1, and A3) play an important role in proliferation, although the functional overlap among members of hnRNPs A/B remains largely unknown. In this study, we have employed RNAi knockdown and proteomic approaches to investigate the biological functions of hnRNPs A/B. Depletion of hnRNP A2, but not A1 or A3, produced a significant inhibition of cellular proliferation in Jurkat cells. Analysis of the proteomes in the cells depleted for hnRNP A1, A2, or A3 has identified a total of 167 differentially expressed proteins in the depleted cells. Network analysis of the proteins altered in the cells depleted for hnRNP A2 revealed that the biological processes likely affected by these proteins are related to cell cycle, cytoskeleton rearrangement, and transcription regulation. Indeed, we have confirmed that the level of RhoA and CrkL was selectively reduced in the cells depleted of hnRNP A2, but not in the cells depleted for hnRNP A1 or A3. Therefore, we suggest that the reduced proliferation observed in the cells depleted of hnRNP A2 may result from its effects on cell adhesion processes in the Jurkat cells.
authors.affiliation: Department of Molecular and Cellular Biology, College of Medicine, Chang Gung University, Tao-Yuan, Taiwan; Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Tao-Yuan, Taiwan.
authors.forename: Yuan-Ming
authors.initials: YM
authors.lastname: Yeh
authors.forename: Chi-Yuan
authors.initials: CY
authors.lastname: Chen
authors.forename: Pei-Rong
authors.initials: PR
authors.lastname: Huang
authors.forename: Chia-Wei
authors.initials: CW
authors.lastname: Hsu
authors.forename: Chih-Ching
authors.initials: CC
authors.lastname: Wu
authors.forename: Tzu-Chien V
authors.initials: TC
authors.lastname: Wang
mesh_terms: D049109:Cell Proliferation; D004867:Equipment Design; D005786:Gene Expression Regulation; D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D019169:Jurkat Cells / Q000166:cytology / Q000378:metabolism*; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods; D020543:Proteome / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics / Q000295:instrumentation / Q000379:methods; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics
chemical_list: D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B; D020543:Proteome; D034741:RNA, Small Interfering
keywords: Cell proliferation; Jurkat cells; Quantitative proteomics; hnRNP A1, A2/B1, A3