title: Core promoter regulates the expression of cathepsin B gene in the fat body of Bombyx mori.
abstract: Bombyx mori cathepsin B (BmCatB) is involved in the programmed cell death of the fat body during B. mori metamorphosis. For a better understanding of the functional regulatory mechanism, the promoter region of BmCatB in the transcriptional regulation has been identified and analyzed in the present study. BmCatB promoter region performed by the 5' truncation or mutagenesis of EcREs was inserted in the pFA3Luc-A3RL double fluorescence expression vector to activate the fireflies luciferase (FLuc) gene. The results indicated that the dual-luciferase activity of BmCatB gene in the silkworm larval fat body is regulated by the length of promoter. Site-directed mutagenesis of EcRE experiment has shown that the EcREs are up-regulated significantly in the regulation of the BmCatB promoter. A 142bp region (-1165 to -1023) and EcREs are the mainly fat-body tissue-specificity related region and could function as a core promoter element.
authors.affiliation: Jiangsu University of Science and Technology, Zhenjiang 212018, China.
authors.forename: Xiang-Yun
authors.initials: XY
authors.lastname: Cai
authors.affiliation: Jiangsu University of Science and Technology, Zhenjiang 212018, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Yu
authors.affiliation: Jiangsu University of Science and Technology, Zhenjiang 212018, China.
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Yu
authors.affiliation: Jiangsu University of Science and Technology, Zhenjiang 212018, China.
authors.forename: Yan-Wei
authors.initials: YW
authors.lastname: Liu
authors.affiliation: Jiangsu University of Science and Technology, Zhenjiang 212018, China.
authors.forename: Yin
authors.initials: Y
authors.lastname: Fang
authors.affiliation: Jiangsu University of Science and Technology, Zhenjiang 212018, China.
authors.forename: Zi-Xu
authors.initials: ZX
authors.lastname: Ren
authors.affiliation: Jiangsu University of Science and Technology, Zhenjiang 212018, China; Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, China.
authors.forename: Jun-Qiang
authors.initials: JQ
authors.lastname: Jia
authors.affiliation: Jiangsu University of Science and Technology, Zhenjiang 212018, China; Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, China.
authors.forename: Guo-Zheng
authors.initials: GZ
authors.lastname: Zhang
authors.affiliation: Jiangsu University of Science and Technology, Zhenjiang 212018, China; Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, China.
authors.forename: Xi-Jie
authors.initials: XJ
authors.lastname: Guo
authors.affiliation: College of Natural Resources and Life Science, Dong-A University, Busan 604-714, Republic of Korea.
authors.forename: Byung-Rae
authors.initials: BR
authors.lastname: Jin
authors.affiliation: Jiangsu University of Science and Technology, Zhenjiang 212018, China; Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, China. Electronic address: srizzgui@hotmail.com.
authors.forename: Zhong-Zheng
authors.initials: ZZ
authors.lastname: Gui
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D012831:Bombyx / Q000235:genetics* / Q000502:physiology; D002401:Cathepsin B / Q000235:genetics* / Q000378:metabolism; D005216:Fat Body / Q000502:physiology*; D005786:Gene Expression Regulation; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D008156:Luciferases / Q000235:genetics; D008675:Metamorphosis, Biological / Q000235:genetics; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009928:Organ Specificity; D011401:Promoter Regions, Genetic
chemical_list: D019476:Insect Proteins; D008156:Luciferases; D002401:Cathepsin B
keywords: Bombyx mori; Cathepsin B promoter; Dual-luciferase expression system; Regulatory elements