title: Water soluble sulfated-fucans with immune-enhancing properties from Ecklonia cava.
abstract: Water-soluble sulfated fucans isolated from Ecklonia cava were fractionated using an anion-exchange chromatography to investigate their molecular characteristics and immunomodulating activities. The crude fucoidan extract and purified fractions (EF1, EF2, and EF3) consisted mostly of different ratios of neutral sugars, proteins, sulfates, uronic acids, and their monosaccharide compositions were also significantly different. The backbone of the most immunoenhancing fraction, EF2, was mainly linked by (1→3)-linked fucopyranosyl and (1→4)-linked mannopyranosyl residues with sulfates at C-4 of fucopyranosyl units. The molecular weights of the crude fucoidan extract and purified fractions ranged from 8.3×10(3) to 442.6×10(3)g/mol. The crude extract, EF1 and EF2 stimulated RAW264.7 cells to produce considerable amounts of nitric oxide and cytokines. The treatment of cells with the sulfated fucans induced the degradation of Iκ-B and the phosphorylation of MAPK in RAW264.7 cells, implying that they might stimulate RAW264.7 cells through the activation of NF-κB and MAPK pathways.
authors.affiliation: College of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, China; Department of Marine Food Science and Technology, Gangneung-Wonju National University, 120 Gangneung Daehangno, Gangneung, Gangwon 210-702, South Korea.
authors.forename: Rong-An
authors.initials: RA
authors.lastname: Cao
authors.affiliation: Chuncheon Bioindustry Foundation, Chuncheon, Gangwon 200-957, South Korea. Electronic address: yjlee67123@hanmail.net.
authors.forename: YongJin
authors.initials: Y
authors.lastname: Lee
authors.affiliation: Department of Marine Food Science and Technology, Gangneung-Wonju National University, 120 Gangneung Daehangno, Gangneung, Gangwon 210-702, South Korea. Electronic address: umyousg@gwnu.ac.kr.
authors.forename: SangGuan
authors.initials: S
authors.lastname: You
mesh_terms: D000818:Animals; D002460:Cell Line / Q000187:drug effects; D007155:Immunologic Factors / Q000737:chemistry / Q000302:isolation & purification*; D051379:Mice; D016328:NF-kappa B / Q000096:biosynthesis; D009569:Nitric Oxide / Q000096:biosynthesis; D000459:Phaeophyceae / Q000737:chemistry / Q000276:immunology*; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification; D012995:Solubility; D014867:Water / Q000737:chemistry
chemical_list: D007155:Immunologic Factors; D016328:NF-kappa B; D010936:Plant Extracts; D011134:Polysaccharides; D014867:Water; D009569:Nitric Oxide; C007789:fucoidan
keywords: Ecklonia cava; Immune-enhancing; MAPK; NF-κB; Sulfate-fucans