title: Overexpression of B7-H3 in CD133+ colorectal cancer cells is associated with cancer progression and survival in human patients.
abstract: Cancer stem-like cells are enriched in CD133-positive (CD133(+)) colorectal cancer (CRC) cells. To date, the biological significance of CD133 expression in cancer stem-like cells is still unknown. B7-H3, a costimulatory molecule, plays a pivotal role in tumor immune escape by inhibiting the functions of T cells. To identify a new marker to predict the tumor grade of CRC, we analyzed the expression of B7-H3 and CD133 in colorectal tumor samples, and their clinical significance was determined. By using a series of techniques including pathologic tissue microarray technology, immunohistochemistry, and immunofluorescent staining, we found B7-H3 was expressed in 56.73% of the CRC cases (59/104) sampled; CD133 was detected in 26.92% of the CRC cases (28/104) sampled. Further analysis indicated that 22 of these CD133(+) samples expressed B7-H3. We also found coexpression of CD133 and B7-H3 in tumor tissue samples (r = 0.321, P < 0.01). Moreover, in contrast to individual CD133 or B7-H3 expression, the coexpression of B7-H3 and CD133 was evidently associated with the depth of tumor invasion, lymphatic metastasis, distant metastasis, and Dukes' stage, suggesting it is a valuable biomarker for the progression of CRC. Indeed, the patients with coexpression of B7-H3 and CD133 had a poorer survival than the other patients (P < 0.05). In summary, our results reveal that B7-H3 was aberrantly expressed in CD133(+) CRC cells, and the expression level was closely associated with tumor progression.
authors.affiliation: Department of Immunology, School of Basic Medical Sciences, Soochow University, Suzhou, People's Republic of China; Institute of Clinical Immunology of Jiangsu Province, The First Affiliated of Soochow University, Suzhou, People's Republic of China; Stem cell Research Laboratory of Jiangsu Province, Soochow University, Suzhou, People's Republic of China.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Bin
authors.affiliation: Institute of Clinical Immunology of Jiangsu Province, The First Affiliated of Soochow University, Suzhou, People's Republic of China.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Guangbo
authors.affiliation: Department of Immunology, School of Basic Medical Sciences, Soochow University, Suzhou, People's Republic of China; Stem cell Research Laboratory of Jiangsu Province, Soochow University, Suzhou, People's Republic of China.
authors.forename: Ge
authors.initials: G
authors.lastname: Yan
authors.affiliation: Department of Immunology, School of Basic Medical Sciences, Soochow University, Suzhou, People's Republic of China; Stem cell Research Laboratory of Jiangsu Province, Soochow University, Suzhou, People's Republic of China.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Huan
authors.affiliation: Department of Immunology, School of Basic Medical Sciences, Soochow University, Suzhou, People's Republic of China; Stem cell Research Laboratory of Jiangsu Province, Soochow University, Suzhou, People's Republic of China. Electronic address: xueguangzh@126.com.
authors.forename: Lv
authors.initials: L
authors.lastname: Desheng
authors.affiliation: Department of Immunology, School of Basic Medical Sciences, Soochow University, Suzhou, People's Republic of China; Institute of Clinical Immunology of Jiangsu Province, The First Affiliated of Soochow University, Suzhou, People's Republic of China; Stem cell Research Laboratory of Jiangsu Province, Soochow University, Suzhou, People's Republic of China. Electronic address: xueguangzh@126.com.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Xueguang
mesh_terms: D000071916:AC133 Antigen; D000230:Adenocarcinoma / Q000235:genetics* / Q000401:mortality / Q000473:pathology*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000096:biosynthesis / Q000235:genetics*; D060887:B7 Antigens / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D003106:Colon / Q000473:pathology; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology*; D018450:Disease Progression; D005260:Female; D006023:Glycoproteins / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D008207:Lymphatic Metastasis / Q000235:genetics; D008297:Male; D008875:Middle Aged; D010455:Peptides / Q000235:genetics*; D012007:Rectum / Q000473:pathology
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D060887:B7 Antigens; C423237:CD276 protein, human; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides
keywords: B7-H3; CD133; Clinicopathology; Colorectal cancer