title: Effect of skin metabolism on dermal delivery of testosterone: qualitative assessment using a new short-term skin model.
abstract: The skin is a metabolically active organ expressing biotransformation enzymes able to metabolize both endogenous molecules and xenobiotics. We investigated the impact of metabolism on the delivery of testosterone through the skin with an ex vivo pig ear skin system as an alternative model for human skin. Penetration, absorption and metabolic capabilities were investigated up to 72 h after application of [(14)C]-testosterone doses of 50-800 nmol on either fresh or frozen skin, with the latter model being metabolically inactive. Testosterone absorption and metabolite production were monitored by radio-HPLC and gas chromatography-mass spectrometry. Testosterone absorption through frozen skin was much lower, irrespective of the dose of testosterone applied, compared to fresh skin. Using fresh skin samples, >95% of the radioactivity recovered in culture media, as well as the skin itself, corresponded to metabolites. These results were compared with the metabolic data obtained from other in vitro systems (liver and skin microsomes). The present work leads to the conclusion that most of the enzymatic activities expressed in liver fractions are also expressed in pig and human skin. The metabolic activity of the skin can modulate the biological activity of pharmaceuticals (and xenobiotics). Consequently, it can also greatly affect transdermal drug delivery.
authors.affiliation: INRA, Toxalim (Research Center in Food Toxicology), Université de Toulouse, Toulouse, France.
authors.forename: C
authors.initials: C
authors.lastname: Jacques
authors.forename: E
authors.initials: E
authors.lastname: Perdu
authors.forename: E L
authors.initials: EL
authors.lastname: Jamin
authors.forename: J P
authors.initials: JP
authors.lastname: Cravedi
authors.forename: A
authors.initials: A
authors.lastname: Mavon
authors.forename: H
authors.initials: H
authors.lastname: Duplan
authors.forename: D
authors.initials: D
authors.lastname: Zalko
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D008862:Microsomes, Liver / Q000378:metabolism; D012867:Skin / Q000378:metabolism*; D012869:Skin Absorption; D013552:Swine; D013739:Testosterone / Q000378:metabolism*
chemical_list: D003470:Culture Media; D013739:Testosterone