title: Specific 12α-hydroxylation of grandiflorenic acid by permeabilised fungus Fusarium graminearum.
abstract: Biotransformation of grandiflorenic acid by permeabilised fungus Fusarium graminearum to yield its hydroxylation derivative, 12α-hydroxygrandiflorenic acid, was studied. The biotransformed product was isolated by column chromatography and its structure was determined by mass spectrum and nuclear magnetic resonance analysis. Grandiflorenic acid was efficiently metabolised by the fungus. After 72 h, the substrate was almost completely converted into the product.
authors.affiliation: a Department of Traditional Chinese Medicine , College of Agronomy, Henan Agricultural University , Zhengzhou 450002 , P.R. China.
authors.forename: Y
authors.initials: Y
authors.lastname: Ruan
authors.forename: B J
authors.initials: BJ
authors.lastname: Ma
authors.forename: L Z
authors.initials: LZ
authors.lastname: Li
authors.forename: X L
authors.initials: XL
authors.lastname: Wang
mesh_terms: D001711:Biotransformation; D004224:Diterpenes / Q000737:chemistry*; D005670:Fusarium / Q000378:metabolism*; D006900:Hydroxylation; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular
chemical_list: C000591776:12alpha-hydroxygrandiflorenic acid; D004224:Diterpenes; C014576:grandiflorenic acid
keywords: 12α-hydroxygrandiflorenic acid; Fusarium graminearum; biotransformation; grandiflorenic acid; hydroxylation