title: USF-1 inhibition protects against oxygen-and-glucose-deprivation-induced apoptosis via the downregulation of miR-132 in HepG2 cells.
abstract: Upstream stimulatory factor 1 (USF-1) is an important transcription factor that participates in glucose metabolism and tumorigenesis. The aim of the current study was to explore the regulatory mechanism of USF-1 in HepG2 cells exposed to oxygen and glucose deprivation (OGD). After the establishment of the OGD model in HepG2 cells, we determined that the cells treated with OGD exhibited a high apoptotic rate and that the introduction of siRNA against USF-1 protected the cells from OGD-induced apoptosis. The miRNA microarray results demonstrated that a set of miRNAs were deregulated in the cells transfected with USF-1 siRNA, and the set of downregulated miRNAs included a novel miRNA, miR-132. Further analyses indicated that miR-132 overexpression inhibits the protective roles of USF-1 siRNA in OGD-induced apoptosis. We also identified several binding sites for USF-1 in the miR-132 promoter. The silencing of USF-1 resulted in a reduction in miR-132 expression, and USF-1 overexpression increased the expression of this miRNA. Our study indicated that the silencing of USF-1 plays protective roles in OGD-induced apoptosis through the downregulation of miR-132, which indicates that the silencing of USF-1 may be a therapeutic strategy for the promotion of cancer cell survival under OGD conditions.
authors.affiliation: Department of Clinical Laboratory, Pingjin Hospital, Logistics College of Armed Police Forces, Tianjin, China.
authors.forename: Renjie
authors.initials: R
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, Pingjin Hospital, Logistics College of Armed Police Forces, Tianjin, China.
authors.forename: Haiqian
authors.initials: H
authors.lastname: Liang
authors.affiliation: Department of Thoracic Surgery, Shandong Cancer Hospital and Institute, Jinan, Shandong, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Clinical Laboratory, Pingjin Hospital, Logistics College of Armed Police Forces, Tianjin, China.
authors.forename: Herong
authors.initials: H
authors.lastname: Dou
authors.affiliation: Department of Clinical Laboratory, Pingjin Hospital, Logistics College of Armed Police Forces, Tianjin, China.
authors.forename: Minghua
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Department of Clinical Laboratory, Pingjin Hospital, Logistics College of Armed Police Forces, Tianjin, China.
authors.lastname: Baobuhe
authors.affiliation: Department of Clinical Laboratory, Pingjin Hospital, Logistics College of Armed Police Forces, Tianjin, China.
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Du
authors.affiliation: Department of Clinical Laboratory, Pingjin Hospital, Logistics College of Armed Police Forces, Tianjin, China.
authors.forename: Mojie
authors.initials: M
authors.lastname: Gao
authors.affiliation: Department of Clinical Laboratory, Pingjin Hospital, Logistics College of Armed Police Forces, Tianjin, China. Electronic address: wrm20142233@sohu.com.
authors.forename: Ruimin
authors.initials: R
authors.lastname: Wang
mesh_terms: D017209:Apoptosis; D015536:Down-Regulation; D005947:Glucose / Q000378:metabolism*; D056945:Hep G2 Cells; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D010100:Oxygen / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D051720:Upstream Stimulatory Factors / Q000235:genetics*
chemical_list: C547077:MIRN132 microRNA, human; D035683:MicroRNAs; D034741:RNA, Small Interfering; C497181:USF1 protein, human; D051720:Upstream Stimulatory Factors; D005947:Glucose; D010100:Oxygen
keywords: Apoptosis; Hepg2 cells; Oxygen and glucose deprivation; Upstream stimulatory factor 1; miR-132