title: Curcumin loaded poly (lactic-co-glycolic) acid nanofiber for the treatment of carcinoma.
abstract: Poly (DL-lactic-co-glycolic) acid [PLGA] copolymers with different ratios (78/22, 68/32 and 61/39) and molecular weight (15,400, 11,000 and 10,000 Da) were synthesized and characterized by (1)H NMR, FTIR, GPC and TGA-DTA studies. Curcumin loaded PLGA with the size of 100-300 nm were obtained by electrospinning in which no visible aggregation observed on the surface. The diameter of CPNF (61/39) nanofiber obtained from the topographical imaging by AFM is 160±10 nm. The water contact angle measurements indicate that an increase in GA content results in increase in the hydrophilicity of the PLGA copolymer. The in vitro release profile and release kinetics from the CPNF demonstrated a sustained release of curcumin from CPNF. The release profile follows Korsmeyer-Peppas model suggesting a combination of surface drug dissolution and non-Fickian diffusion as a major drug release mechanism. The effect of CPNF on cell viability was assessed by the MTT (3-[4,5-dimethylthiazol-2-yl] 2,5-diphenyltetrazolium bromide) assay to examine the cytotoxic effect of released curcumin on A431 cells in vitro.
authors.affiliation: Department of Inorganic Chemistry, University of Madras, Guindy Campus, Chennai 600 025, India.
authors.forename: Malathi
authors.initials: M
authors.lastname: Sampath
authors.affiliation: Biomaterials division, CSIR-CLRI, TICEL Biopark, Chennai 600 113, India.
authors.forename: Rachita
authors.initials: R
authors.lastname: Lakra
authors.affiliation: Biomaterials division, CSIR-CLRI, TICEL Biopark, Chennai 600 113, India.
authors.forename: Purnasai
authors.initials: P
authors.lastname: Korrapati
authors.affiliation: Department of Inorganic Chemistry, University of Madras, Guindy Campus, Chennai 600 025, India. Electronic address: bala2010@yahoo.com.
authors.forename: Balasubramanian
authors.initials: B
authors.lastname: Sengottuvelan
mesh_terms: D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D003474:Curcumin / Q000494:pharmacology / Q000627:therapeutic use*; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D008970:Molecular Weight; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D009369:Neoplasms / Q000188:drug therapy* / Q000473:pathology; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D017550:Spectroscopy, Fourier Transform Infrared; D014783:Viscosity; D014867:Water / Q000737:chemistry
chemical_list: D014867:Water; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D003474:Curcumin
keywords: Carcinoma; Curcumin; Electrospinning; Melt polycondensation; Nanofiber; Poly (dl-lactic-co-glycolic) acid