title: Simple and rapid assay method for simultaneous quantification of urinary nicotine and cotinine using micro-extraction by packed sorbent and gas chromatography-mass spectrometry.
abstract: A simple and rapid method for determination of nicotine and cotinine levels in urine was developed using samples prepared by micro-extraction by packed sorbent (MEPS) and subjected to gas chromatography-mass spectrometry (GC-MS) analysis. This method provided good reproducibility, as well as good linearity of calibration curves in the range of 1-100 and 50-1000 ng/mL for quality control samples spiked with nicotine and cotinine, respectively. The detection limit of nicotine and cotinine was as low as 0.25 and 20 ng/mL, respectively. An evaporation procedure is not suitable for nicotine determination, thus an advantage of the present MEPS assay method is direct testing with GC-MS without the need for evaporation to a dry solvent. Our findings show that it may be useful for determining nicotine levels in various types of research studies.
authors.forename: Masae
authors.initials: M
authors.lastname: Iwai
authors.forename: Tadashi
authors.initials: T
authors.lastname: Ogawa
authors.forename: Hideki
authors.initials: H
authors.lastname: Hattori
authors.forename: Kei
authors.initials: K
authors.lastname: Zaitsu
authors.forename: Akira
authors.initials: A
authors.lastname: Ishii
authors.forename: Osamu
authors.initials: O
authors.lastname: Suzuki
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Seno
mesh_terms: D002138:Calibration; D003367:Cotinine / Q000652:urine*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D005740:Gases; D006801:Humans; D007477:Ions; D008297:Male; D008875:Middle Aged; D009538:Nicotine / Q000737:chemistry / Q000652:urine*; D015203:Reproducibility of Results; D012907:Smoking / Q000652:urine*; D012997:Solvents / Q000737:chemistry
chemical_list: D005740:Gases; D007477:Ions; D012997:Solvents; D009538:Nicotine; D003367:Cotinine