title: Magnetic-composite-modified polycrystalline silicon nanowire field-effect transistor for vascular endothelial growth factor detection and cancer diagnosis.
abstract: This study proposes a vascular endothelial growth factor (VEGF) biosensor for diagnosing various stages of cervical carcinoma. In addition, VEGF concentrations at various stages of cancer therapy are determined and compared to data obtained by computed tomography (CT) and cancer antigen 125 (CA-125). The increase in VEGF concentrations during operations offers useful insight into dosage timing during cancer therapy. This biosensor uses Avastin as the biorecognition element for the potential cancer biomarker VEGF and is based on a n-type polycrystalline silicon nanowire field-effect transistor (poly-SiNW-FET). Magnetic nanoparticles with poly[aniline-co-N-(1-one-butyric acid) aniline]-Fe3O4 (SPAnH-Fe3O4) shell-core structures are used as carriers for Avastin loading and provide rapid purification due to their magnetic properties, which prevent the loss of bioactivity; furthermore, the high surface area of these structures increases the quantity of Avastin immobilized. Average concentrations in human blood for species that interfere with detection specificity are also evaluated. The detection range of the biosensor for serum samples covers the results expected from both healthy individuals and cancer patients.
authors.affiliation: Department of Chemical and Materials Engineering and Biosensor Group, Biomedical Engineering Research Center, Chang Gung University , Taoyuan 33302, Taiwan, Republic of China.
authors.forename: Hsiao-Chien
authors.initials: HC
authors.lastname: Chen
authors.forename: Jian-Tai
authors.initials: JT
authors.lastname: Qiu
authors.forename: Fu-Liang
authors.initials: FL
authors.lastname: Yang
authors.forename: Yin-Chih
authors.initials: YC
authors.lastname: Liu
authors.forename: Min-Cheng
authors.initials: MC
authors.lastname: Chen
authors.forename: Rung-Ywan
authors.initials: RY
authors.lastname: Tsai
authors.forename: Hung-Wei
authors.initials: HW
authors.lastname: Yang
authors.forename: Chia-Yi
authors.initials: CY
authors.lastname: Lin
authors.forename: Chu-Chi
authors.initials: CC
authors.lastname: Lin
authors.forename: Tzong-Shoon
authors.initials: TS
authors.lastname: Wu
authors.forename: Yi-Ming
authors.initials: YM
authors.lastname: Tu
authors.forename: Min-Cong
authors.initials: MC
authors.lastname: Xiao
authors.forename: Chia-Hua
authors.initials: CH
authors.lastname: Ho
authors.forename: Chien-Chao
authors.initials: CC
authors.lastname: Huang
authors.forename: Chao-Sung
authors.initials: CS
authors.lastname: Lai
authors.forename: Mu-Yi
authors.initials: MY
authors.lastname: Hua
mesh_terms: D061067:Antibodies, Monoclonal, Humanized / Q000737:chemistry* / Q000276:immunology; D000068258:Bevacizumab; D015374:Biosensing Techniques; D018394:CA-125 Antigen / Q000032:analysis / Q000097:blood*; D002277:Carcinoma / Q000097:blood / Q000175:diagnosis* / Q000276:immunology / Q000473:pathology; D005260:Female; D052203:Ferrosoferric Oxide / Q000737:chemistry; D006801:Humans; D059346:Magnets; D008565:Membrane Proteins / Q000032:analysis / Q000097:blood*; D053770:Nanowires / Q000737:chemistry; D009367:Neoplasm Staging; D012825:Silicon / Q000737:chemistry; D014057:Tomography, X-Ray Computed; D014173:Transistors, Electronic; D002583:Uterine Cervical Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000276:immunology / Q000473:pathology; D042461:Vascular Endothelial Growth Factor A / Q000097:blood*
chemical_list: D061067:Antibodies, Monoclonal, Humanized; D018394:CA-125 Antigen; C451759:MUC16 protein, human; D008565:Membrane Proteins; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D000068258:Bevacizumab; D052203:Ferrosoferric Oxide; D012825:Silicon