title: Serotonin induces the migration of PC12 cells via the serotonin receptor 6/cAMP/ERK pathway.
abstract: Serotonin (5-HT) functions as a chemoattractant that modulates neural migration during prenatal and early postnatal development. However, its molecular mechanism remains to be elucidated. The effect of 5-HT on neural cell migration was examined using PC12 neuron-like cell line. Transwell migration assay was used to determine the effect of 5-HT on PC12 cell migration. The results demonstrated that 5-HT and nerve growth factor (NGF) induced PC12 cell migration in a dose-dependent manner. Additionally, 5-HT receptor antagonists suggest that 5-HT-induced migration was mediated by serotonin receptor 6 (5-HT6), a Gs-protein coupled receptor that elevates the intercellular cAMP level. By contrast, antagonists of serotonin receptor 3 (5-HT3) did not show any effects on PC12 cell migration. Clozapine, an inhibitor of cAMP accumulation mediated by 5-HT6, significantly reduced the effect of 5-HT on the PC12 cell migration. An inhibitor of extracellular signal-regulated kinase (ERK) also suppressed migration. These results suggest that 5-HT induces PC12 cell migration by activating cAMP/ERK signaling pathways, which is mediated by 5-HT6receptor.
authors.affiliation: Center for Child Mental Development, Kanazawa University, Kanazawa, Ishikawa, Japan.
authors.forename: Keita
authors.initials: K
authors.lastname: Koizumi
authors.affiliation: Department of Oncology, Ageo Central General Hospital, Ageo, Saitama, Japan ; Center for AIDS Research, Kumamoto University, Kumamoto, Japan.
authors.forename: Hideo
authors.initials: H
authors.lastname: Nakajima
keywords: neural migration; serotonin; serotonin receptor 6