title: Preparation of monolithic affinity media for nano-liquid chromatography applications.
abstract: In this protocol, a strategy is described for preparing affinity media with monolithic materials as stationary phase, which is exemplified for the biotin-avidin interaction pair. The capillary columns prepared in this manner are compatible with nano-liquid chromatographic conditions. Our protocol is easily adapted to the preparation of specific affinity media with different functionalities and as such provides a platform for a multitude of applications.
authors.affiliation: Department of Pharmaceutical Chemistry & Bioanalytics, Institute of Pharmacy, Martin-Luther University Halle-Wittenberg, Wolfgang-Langenbeck-Str. 4, 06120, Halle (Saale), Germany, j.spross@uni-bielefeld.de.
authors.forename: Jens
authors.initials: J
authors.lastname: Sproß
authors.forename: Andrea
authors.initials: A
authors.lastname: Sinz
mesh_terms: D002846:Chromatography, Affinity / Q000379:methods*; D036103:Nanotechnology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization