title: Enhanced cellular responses to titanium coating with hierarchical hybrid structure.
abstract: In this work, nano/micro hierarchical hybrid structured surface was prepared by fabricating a titania nanotube layer in plasma sprayed porous titanium coating (TC). In vitro human marrow stem cells (hMSCs) were employed for the evaluation of the biological properties of the anodized titanium coating with a hierarchical structure (HSTC). Significantly higher cell adhesion quantity (about 30% more) was found on the HSTC than that on the as-sprayed TC. The expressions of osteocalcin (OC) and osteopontin (OPN) for the HSTC were also detected to be about twice as high as those on the as-sprayed TC. The enhanced cell responses on the HSTC were explained by the improved protein adhesion resulted from the increased surface area and surface energy. Combining the advantages in the mechanical fixation and long-term stability of the plasma sprayed porous TC, the HSTC with a hierarchical structure may be a good candidate for hard tissue replacements, especially for load-bearing implants.
authors.affiliation: Shanghai Institute of Ceramics, Key Laboratory of Inorganic Coating Materials, Chinese Academy of Sciences, Shanghai 200050, PR China. Electronic address: xieyoutao@mail.sic.ac.cn.
authors.forename: Youtao
authors.initials: Y
authors.lastname: Xie
authors.affiliation: Shanghai Institute of Ceramics, Key Laboratory of Inorganic Coating Materials, Chinese Academy of Sciences, Shanghai 200050, PR China.
authors.forename: Haiyong
authors.initials: H
authors.lastname: Ao
authors.affiliation: Shanghai Institute of Ceramics, Key Laboratory of Inorganic Coating Materials, Chinese Academy of Sciences, Shanghai 200050, PR China.
authors.forename: Shigang
authors.initials: S
authors.lastname: Xin
authors.affiliation: Shanghai Institute of Ceramics, Key Laboratory of Inorganic Coating Materials, Chinese Academy of Sciences, Shanghai 200050, PR China. Electronic address: xbzheng@mail.sic.ac.cn.
authors.forename: Xuebin
authors.initials: X
authors.lastname: Zheng
authors.affiliation: Shanghai Institute of Ceramics, Key Laboratory of Inorganic Coating Materials, Chinese Academy of Sciences, Shanghai 200050, PR China.
authors.forename: Chuanxian
authors.initials: C
authors.lastname: Ding
mesh_terms: D000042:Absorption; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D014025:Titanium / Q000494:pharmacology*
chemical_list: D020099:Coated Materials, Biocompatible; C009495:titanium dioxide; D014025:Titanium
keywords: Cellular responses; Hierarchical structure; Nanotube; Titanium coating