title: Remote-controlled robotic platform ORPHEUS as a new tool for detection of bacteria in the environment.
abstract: Remote-controlled robotic systems are being used for analysis of various types of analytes in hostile environment including those called extraterrestrial. The aim of our study was to develop a remote-controlled robotic platform (ORPHEUS-HOPE) for bacterial detection. For the platform ORPHEUS-HOPE a 3D printed flow chip was designed and created with a culture chamber with volume 600 μL. The flow rate was optimized to 500 μL/min. The chip was tested primarily for detection of 1-naphthol by differential pulse voltammetry with detection limit (S/N = 3) as 20 nM. Further, the way how to capture bacteria was optimized. To capture bacterial cells (Staphylococcus aureus), maghemite nanoparticles (1 mg/mL) were prepared and modified with collagen, glucose, graphene, gold, hyaluronic acid, and graphene with gold or graphene with glucose (20 mg/mL). The most up to 50% of the bacteria were captured by graphene nanoparticles modified with glucose. The detection limit of the whole assay, which included capturing of bacteria and their detection under remote control operation, was estimated as 30 bacteria per μL.
authors.affiliation: Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Czech Republic.
authors.forename: Lukas
authors.initials: L
authors.lastname: Nejdl
authors.forename: Jiri
authors.initials: J
authors.lastname: Kudr
authors.forename: Kristyna
authors.initials: K
authors.lastname: Cihalova
authors.forename: Dagmar
authors.initials: D
authors.lastname: Chudobova
authors.forename: Michal
authors.initials: M
authors.lastname: Zurek
authors.forename: Ludek
authors.initials: L
authors.lastname: Zalud
authors.forename: Lukas
authors.initials: L
authors.lastname: Kopecny
authors.forename: Frantisek
authors.initials: F
authors.lastname: Burian
authors.forename: Branislav
authors.initials: B
authors.lastname: Ruttkay-Nedecky
authors.forename: Sona
authors.initials: S
authors.lastname: Krizkova
authors.forename: Marie
authors.initials: M
authors.lastname: Konecna
authors.forename: David
authors.initials: D
authors.lastname: Hynek
authors.forename: Pavel
authors.initials: P
authors.lastname: Kopel
authors.forename: Jan
authors.initials: J
authors.lastname: Prasek
authors.forename: Vojtech
authors.initials: V
authors.lastname: Adam
authors.forename: Rene
authors.initials: R
authors.lastname: Kizek
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D055664:Electrochemical Techniques / Q000295:instrumentation; D004783:Environmental Microbiology; D004867:Equipment Design; D004926:Escherichia coli / Q000201:enzymology / Q000302:isolation & purification; D057230:Limit of Detection; D058185:Magnetite Nanoparticles / Q000737:chemistry; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D009284:Naphthols / Q000302:isolation & purification; D058998:Remote Sensing Technology / Q000295:instrumentation*; D012371:Robotics / Q000295:instrumentation; D013211:Staphylococcus aureus / Q000201:enzymology / Q000302:isolation & purification*
chemical_list: D058185:Magnetite Nanoparticles; D009284:Naphthols; C029350:1-naphthol; D000469:Alkaline Phosphatase
keywords: 1-Naphthyl phosphate; Alkaline phosphatase; Bacteria; Electrochemical detection; Magnetic particles; Planetary science; Remote sensing