title: Development of a DNA-based vaccine strategy against bovine papillomavirus infection, involving the E5 or L2 gene.
abstract: Papillomaviruses are known to cause tumor lesions, generally benign, in epithelial tissues of diverse organisms; these lesions may progress to cancer under suitable conditions. Bovine papillomavirus (BPV) can cause urinary bladder cancer and cancer of the upper gastrointestinal tract. Furthermore, BPV1 and BPV2 are implicated in the development of tumors in equids. Many studies with animal models clearly demonstrate that DNA vaccines are very effective tools in controlling viral infections, providing strong humoral and cellular immune responses. In this study, we have described the development of two vaccine constructs for the control of diseases caused by BPV. The 1st strategy is prophylactic and is based on the L2 gene; the 2nd is therapeutic and is based on the E5 gene. Vaccine constructs were obtained and evaluated in vitro in mammalian cells. The results show the occurrence of E5 and L2 transcription and viral protein production. These results confirm the functionality of the vaccine constructs in mammalian cells. This is the 1st step in the development of a DNA-based vaccine strategy for the control and/or treatment of diseases caused by BPV.
authors.affiliation: Laboratório de Estudos Moleculares e Terapia Experimental, Departamento de Genética, Universidade Federal de Pernambuco, Recife, PE, Brasil.
authors.forename: E G
authors.initials: EG
authors.lastname: Lima
authors.affiliation: Laboratório de Estudos Moleculares e Terapia Experimental, Departamento de Genética, Universidade Federal de Pernambuco, Recife, PE, Brasil.
authors.forename: R C
authors.initials: RC
authors.lastname: Lira
authors.affiliation: Laboratório de Estudos Moleculares e Terapia Experimental, Departamento de Genética, Universidade Federal de Pernambuco, Recife, PE, Brasil.
authors.forename: A L S
authors.initials: AL
authors.lastname: Jesus
authors.affiliation: Laboratório de Virologia e Terapia Experimental, Centro de Pesquisa Aggeu Magalhães, Recife, PE, Brasil.
authors.forename: R
authors.initials: R
authors.lastname: Dhalia
authors.affiliation: Laboratório de Estudos Moleculares e Terapia Experimental, Departamento de Genética, Universidade Federal de Pernambuco, Recife, PE, Brasil antonio.freitas@pq.cnpq.br.
authors.forename: A C
authors.initials: AC
authors.lastname: Freitas
mesh_terms: D000818:Animals; D052688:Bovine papillomavirus 1 / Q000502:physiology; D036022:Capsid Proteins / Q000235:genetics* / Q000378:metabolism*; D002417:Cattle / Q000821:virology; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D009856:Oncogene Proteins, Viral / Q000235:genetics* / Q000378:metabolism*; D030361:Papillomavirus Infections / Q000517:prevention & control / Q000628:therapy / Q000662:veterinary; D019444:Vaccines, DNA / Q000378:metabolism / Q000627:therapeutic use
chemical_list: D036022:Capsid Proteins; C085218:L2 protein, Bovine papillomavirus; D009856:Oncogene Proteins, Viral; D019444:Vaccines, DNA; C054304:oncogene protein E5, Bovine papillomavirus type 1