title: Enhanced antibody recognition with a magneto-optic surface plasmon resonance (MO-SPR) sensor.
abstract: A comparison between sensing performance of traditional SPR (Surface Plasmon Resonance) and magneto-optic SPR (MOSPR) transducing techniques is presented in this work. MOSPR comes from an evolution of traditional SPR platform aiming at modulating Surface Plasmon wave by the application of an external magnetic field in transverse configuration. Previous work demonstrated that, when the Plasmon resonance is excited in these structures, the external magnetic field induces a modification of the coupling of the incident light with the Surface Plasmon Polaritons (SPP). Besides, these structures can lead to an enhancement in the magneto-optical (MO) activity when the SPP is excited. This phenomenon is exploited in this work to demonstrate the possibility to use the enhanced MO signal as proper transducer signal for investigating biomolecular interactions in liquid phase. To this purpose, the transducer surface was functionalized by thiol chemistry and used for recording the binding between Bovine Serum Albumin molecules immobilized onto the surface and its complementary target. Higher sensing performance in terms of sensitivity and lower limit of detection of the MOSPR biosensor with respect to traditional SPR sensors is demonstrated.
authors.affiliation: CNR-IMM-Institute for Microelectronic and Microsystems, Unit of Lecce, Via per Monteroni, 73100 Lecce, Italy. Electronic address: mariagrazia.manera@le.imm.cnr.it.
authors.forename: Maria Grazia
authors.initials: MG
authors.lastname: Manera
authors.affiliation: IMM-Instituto de Microelectrónica de Madrid (CNM-CSIC), Isaac Newton 8, 28760 Tres Cantos, Madrid, Spain.
authors.forename: Elías
authors.initials: E
authors.lastname: Ferreiro-Vila
authors.affiliation: IMM-Instituto de Microelectrónica de Madrid (CNM-CSIC), Isaac Newton 8, 28760 Tres Cantos, Madrid, Spain.
authors.forename: José Miguel
authors.initials: JM
authors.lastname: Garcia-Martin
authors.affiliation: IMM-Instituto de Microelectrónica de Madrid (CNM-CSIC), Isaac Newton 8, 28760 Tres Cantos, Madrid, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Garcia-Martin
authors.affiliation: CNR-IMM-Institute for Microelectronic and Microsystems, Unit of Lecce, Via per Monteroni, 73100 Lecce, Italy.
authors.forename: Roberto
authors.initials: R
authors.lastname: Rella
mesh_terms: D000818:Animals; D000906:Antibodies / Q000032:analysis* / Q000276:immunology*; D015374:Biosensing Techniques / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation*; D018189:Immunomagnetic Separation / Q000295:instrumentation*; D055096:Optical Devices; D020349:Surface Plasmon Resonance / Q000295:instrumentation*; D014159:Transducers
chemical_list: D000906:Antibodies
keywords: Active plasmonics; Magneto-optic; Surface Plasmon Resonance (SPR); Transducing layer