title: Enhanced stability of alcohol dehydrogenase by non-covalent interaction with polysaccharides.
abstract: Non-covalent interaction of alcohol dehydrogenase with polysaccharides was studied using three neutral and three anionic polysaccharides. The process of interaction of alcohol dehydrogenase with gum Arabic was optimized with respect to the ratio of enzyme to gum Arabic, pH, and molarity of buffer. Alcohol dehydrogenase-gum Arabic complex formed under optimized conditions showed 93% retention of original activity with enhanced thermal and pH stability. Lower inactivation rate constant of alcohol dehydrogenase-gum Arabic complex within the temperature range of 45 to 60 °C implied its better stability. Half-life of alcohol dehydrogenase-gum Arabic complex was higher than that of free alcohol dehydrogenase. A slight increment was observed in kinetic constants (K(m) and V(max)) of gum Arabic-complexed alcohol dehydrogenase which may be due to interference by gum Arabic for the binding of substrate to the enzyme. Helix to turn conversion was observed in complexed alcohol dehydrogenase as compared to free alcohol dehydrogenase which may be responsible for observed stability enhancement.
authors.affiliation: Department of Biotechnology and Chemical Technology, School of Chemical Technology, Aalto University, POB 16100, 00076, Aalto, Finland.
authors.forename: Swati B
authors.initials: SB
authors.lastname: Jadhav
authors.forename: Sandip B
authors.initials: SB
authors.lastname: Bankar
authors.forename: Tom
authors.initials: T
authors.lastname: Granström
authors.forename: Heikki
authors.initials: H
authors.lastname: Ojamo
authors.forename: Rekha S
authors.initials: RS
authors.lastname: Singhal
authors.forename: Shrikant A
authors.initials: SA
authors.lastname: Survase
mesh_terms: D000426:Alcohol Dehydrogenase / Q000737:chemistry* / Q000378:metabolism*; D004795:Enzyme Stability; D006170:Gum Arabic / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D011485:Protein Binding; D013696:Temperature
chemical_list: D006170:Gum Arabic; D000426:Alcohol Dehydrogenase