title: A strategy for generation and balancing of autosome: Y chromosome translocations.
abstract: We describe a method for generation and maintenance of translocations that move large autosomal segments onto the Y chromosome. Using this strategy we produced ( 2;Y) translocations that relocate between 1.5 and 4.8 Mb of the 2nd chromosome.. All translocations were easily balanced over a male-specific lethal 1 (msl-1) mutant chromosome. Both halves of the translocation carry visible markers, as well as P-element ends that enable molecular confirmation. Halves of these translocations can be separated to produce offspring with duplications and with lethal second chromosome deficiencies . Such large deficiencies are otherwise tedious to generate and maintain.
authors.affiliation: Department of Biological Sciences; Wayne State University; Detroit, MI USA.
authors.forename: Sonal S
authors.initials: SS
authors.lastname: Joshi
authors.affiliation: Department of Biological Sciences; Wayne State University; Detroit, MI USA.
authors.forename: Han
authors.initials: H
authors.lastname: Cheong
authors.affiliation: Department of Biological Sciences; Wayne State University; Detroit, MI USA.
authors.forename: Victoria H
authors.initials: VH
authors.lastname: Meller
mesh_terms: D000818:Animals; D059006:Chromosomes, Insect; D004330:Drosophila / Q000235:genetics*; D005260:Female; D005821:Genetic Techniques; D008297:Male; D014178:Translocation, Genetic; D014998:Y Chromosome
keywords: Drosophila melanogaster; Y chromosome; chromosome translocations; deficiencies