title: Anti-inflammatory potential of alpha-linolenic acid mediated through selective COX inhibition: computational and experimental data.
abstract: The present work investigates the anti-inflammatory activity of alpha-linolenic acid (ALA) and linoleic acid (LA) using computational and experimental analysis. The binding affinity of ALA and LA was appraised for cyclooxygenase 1 (COX-1), cyclooxygenase 2 (COX-2), and 5-lipoxygenase (5-LOX) using AutoDock 4.2 and AutoDock Vina 1.1.2. Anti-inflammatory activity of ALA (2 and 4 ml/kg, i.p.) (55.65 % v/v) and LA (2 and 4 ml/kg, i.p.) (55 % v/v) was further assayed using the rat paw edema test against a variety of phlogistic agents including carrageenan, arachidonic acid, prostaglandin, and leukotriene, respectively. ALA (2 and 4 ml/kg, i.p.) and LA (2 and 4 ml/kg, i.p.) were further tested for their efficacy against complete Freund's adjuvant (CFA)-induced (0.05 ml) arthritis in albino rats. Following CFA-induced arthritis, ALA and LA were tested for their inhibitory proficiency against COX-1, COX-2, and 5-LOX in vitro. The present study commends that the anti-inflammatory potential of ALA could be attributed to COX inhibition, in particular, COX-2.
authors.affiliation: Division of Applied Sciences and Indo-Russian Centre for Biotechnology, Indian Institute of Information Technology, Allahabad, 211012, Uttar Pradesh, India.
authors.forename: Richa
authors.initials: R
authors.lastname: Anand
authors.forename: Gaurav
authors.initials: G
authors.lastname: Kaithwas
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry*; D001094:Arachidonate 5-Lipoxygenase / Q000378:metabolism*; D016718:Arachidonic Acid / Q000737:chemistry; D001168:Arthritis / Q000503:physiopathology; D002351:Carrageenan / Q000737:chemistry; D051545:Cyclooxygenase 1 / Q000378:metabolism*; D051546:Cyclooxygenase 2 / Q000378:metabolism*; D016861:Cyclooxygenase Inhibitors / Q000737:chemistry*; D004487:Edema; D015289:Leukotrienes / Q000737:chemistry; D016859:Lipoxygenase Inhibitors / Q000737:chemistry; D062105:Molecular Docking Simulation; D008969:Molecular Sequence Data; D011453:Prostaglandins / Q000737:chemistry; D011487:Protein Conformation; D051381:Rats; D017208:Rats, Wistar; D017962:alpha-Linolenic Acid / Q000737:chemistry*
chemical_list: D000893:Anti-Inflammatory Agents; D016861:Cyclooxygenase Inhibitors; D015289:Leukotrienes; D016859:Lipoxygenase Inhibitors; D011453:Prostaglandins; D017962:alpha-Linolenic Acid; D016718:Arachidonic Acid; D002351:Carrageenan; D001094:Arachidonate 5-Lipoxygenase; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2