title: Bioactivity-guided isolation of anti-inflammation flavonoids from the stems of Millettia dielsiana Harms.
abstract: Bioactivity-guided isolation of the EtOAc extract of the stems of Millettia dielsiana Harms yielded two new isoflavones together with nine known ones. Their structures were elucidated by analysis of the spectroscopic data including 2D NMR. All of the isolates were evaluated for their potential to inhibit the LPS-induced production of nitric oxide and TNF-α in murine macrophage RAW 264.7 cells. Among the tested compounds, Millesianin C (1) had the most potent anti-inflammatory effect decreasing NO production similar to that of dexamethasone and decreasing TNF-α secretion better than that of dexamethasone. Their structure-activity relationship was also analyzed.
authors.affiliation: State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, China; Guangdong Zhongsheng Pharmaceutical Co Ltd, Dongguan 440100, Guangdong, China.
authors.forename: Haoyu
authors.initials: H
authors.lastname: Ye
authors.affiliation: State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, China.
authors.forename: Wenshuang
authors.initials: W
authors.lastname: Wu
authors.affiliation: Guangdong Zhongsheng Pharmaceutical Co Ltd, Dongguan 440100, Guangdong, China.
authors.forename: Zhuowei
authors.initials: Z
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, China.
authors.forename: Caifeng
authors.initials: C
authors.lastname: Xie
authors.affiliation: State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, China.
authors.forename: Minghai
authors.initials: M
authors.lastname: Tang
authors.affiliation: State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, China.
authors.forename: Shucai
authors.initials: S
authors.lastname: Li
authors.affiliation: State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, China.
authors.forename: Jianhong
authors.initials: J
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Tang
authors.affiliation: State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Chen
authors.affiliation: Guangdong Zhongsheng Pharmaceutical Co Ltd, Dongguan 440100, Guangdong, China.
authors.forename: Chaofeng
authors.initials: C
authors.lastname: Long
authors.affiliation: State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, China. Electronic address: peng_aihua@163.com.
authors.forename: Aihua
authors.initials: A
authors.lastname: Peng
authors.affiliation: State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, China. Electronic address: chenlijuan013@sohu.com.
authors.forename: Yuquam
authors.initials: Y
authors.lastname: Wei
authors.affiliation: State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, China.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Chen
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D003907:Dexamethasone / Q000494:pharmacology; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D007529:Isoflavones / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008516:Medicine, Chinese Traditional; D051379:Mice; D033641:Millettia / Q000737:chemistry*; D015394:Molecular Structure; D009569:Nitric Oxide / Q000032:analysis / Q000378:metabolism*; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal; D013329:Structure-Activity Relationship; D014409:Tumor Necrosis Factor-alpha / Q000187:drug effects / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D005419:Flavonoids; D007529:Isoflavones; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; C000594195:millesianin H; C000594196:millesianin I; D009569:Nitric Oxide; D003907:Dexamethasone
keywords: Anti-inflammation; Millettia dielsiana Harms; NO; SAR; TNF-α