title: Alleviation of experimental allergic encephalomyelitis in C57BL/6 mice by soy daidzein.
abstract: Experimental allergic encephalomyelitis (EAE) is considered as the murine model of multiple sclerosis. Daidzein a phytostrogenic compound of soy is known to impose immunomodulatory and antioxidative effects. We conducted this study to assess the potential protective and therapeutic effects of daidzein on allergic encephalomyelitis.C57BL/6 mice were induced with allergic encephalomyelitis using myelin oligodendrocyte glycoprotein (35-55) and received daidzein or dimethyl sulfoxide as the vehicle control. To assess the protective effect of daidzein, the mice were administered with 20 mg/kg of daidzein from 21 days prior to 21 days post EAE induction on a daily basis. To evaluate the therapeutic effect of daidzein, mice were fed with 300 mg/kg daidzein after the appearance of the first clinical signs for 10 days. One day after the last gavage, the mice were sacrificed. Spleen and brain were removed for further histological and immunological analysis.Feeding mice with low dose of daidzein prior to disease induction did not affect disease severity. However, treating with high dose of daidzein after the onset of the disease reduced interferon-γ and interleukin-12 secretion, enhanced interleukin-10 production, suppressed lymphocyte proliferation, and decreased cytotoxicity as judged by lactate dehydrogenase release.In conclusion, daidzein reduced the extent of demyelination and disease severity. Chronic oral therapy with low dose of daidzein did not prevent experimental autoimmune encephalomyelitis. However, high doses of daidzein could prohibit disease exacerbation.
authors.affiliation: Sina Hospital, Multiple Sclerosis Research Center- Neuroscience Institute, Tehran University of Medical Sciences, Tehran, Iran and Shefa Neuroscience Research Center, Tehran, Iran. Razeghi@razi.tums.ac.ir.
authors.forename: Soodeh
authors.initials: S
authors.lastname: Razeghi Jahromi
authors.affiliation: Department of Nutrition and Health, Tabriz University of Medical Sciences, Tabriz, Iran. arrefhosseini@mail.com.
authors.forename: Seyed Rafi
authors.initials: SR
authors.lastname: Arrefhosseini
authors.affiliation: Shefa Neuroscience Research Center, Tehran, Iran and Infectious Diseases Research Center, Department of Microbiology, Golestan University of Medical Sciences, Gorgan, Iran. ghaem_amir@yahoo.com.
authors.forename: Amir
authors.initials: A
authors.lastname: Ghaemi
authors.affiliation: Tissue Engineering Department, Advanced Technology in Medicine,Tehran University of Medical Sciences, Tehran, Iran. Alizadeh.akram@mail.com.
authors.forename: Akram
authors.initials: A
authors.lastname: Alizadeh
authors.affiliation: Department of Pathology, Sina Hospital, Tehran University of Medical Sciences, Tehran, Iran. hmoradi@razi.tums.ac.ir.
authors.forename: Hedieh
authors.initials: H
authors.lastname: Moradi Tabriz
authors.affiliation: Department of Neurology, Sina Hospital, Tehran University of Medical Sciences, Tehran, Iran and Iranian Center of Neurological Research-Neuroscience Institute,Tehran University of Medical Sciences, Tehran, Iran. toghae@sina.tums.ac.ir.
authors.forename: Mansoureh
authors.initials: M
authors.lastname: Togha
mesh_terms: D000818:Animals; D016207:Cytokines / Q000378:metabolism; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000188:drug therapy* / Q000276:immunology; D005260:Female; D007529:Isoflavones / Q000627:therapeutic use*; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D016207:Cytokines; D007529:Isoflavones; C004742:daidzein