title: Selecting low-cost carbon sources for carotenoid and lipid production by the pink yeast Rhodosporidium toruloides NCYC 921 using flow cytometry.
abstract: The present work studied low-cost carbon sources for carotenoid and lipid production using the yeast Rhodosporidum toruloides NCYC 921. Carob pulp syrup and sugarcane molasses at different concentrations were used as low-cost carbon sources in R. toruloides batch cultivations. Carob pulp syrup containing a total sugar concentration of 75 g L(-1) induced the highest total fatty acid productivity (1.90 g L(-1)h(-1)) and the highest carotenoid productivity (9.79 μg L(-1)h(-1)). Flow cytometric analysis revealed that most of the yeast cells (>60%) grown on carob pulp syrup displayed intact polarised membranes, conversely to the cells grown on sugarcane molasses, wherein a large proportion (>45%) displayed permeabilised cytoplasmic membranes.
authors.affiliation: Laboratório Nacional de Energia e Geologia, I.P., Unidade de Bioenergia, Estrada do Paço do Lumiar 22, 1649-038 Lisboa, Portugal.
authors.forename: Cláudia
authors.initials: C
authors.lastname: Freitas
authors.affiliation: Laboratório Nacional de Energia e Geologia, I.P., Unidade de Bioenergia, Estrada do Paço do Lumiar 22, 1649-038 Lisboa, Portugal.
authors.forename: Teresa Margarida
authors.initials: TM
authors.lastname: Parreira
authors.affiliation: Laboratório Nacional de Energia e Geologia, I.P., Unidade de Bioenergia, Estrada do Paço do Lumiar 22, 1649-038 Lisboa, Portugal.
authors.forename: José
authors.initials: J
authors.lastname: Roseiro
authors.affiliation: Laboratório Nacional de Energia e Geologia, I.P., Unidade de Bioenergia, Estrada do Paço do Lumiar 22, 1649-038 Lisboa, Portugal.
authors.forename: Alberto
authors.initials: A
authors.lastname: Reis
authors.affiliation: Laboratório Nacional de Energia e Geologia, I.P., Unidade de Bioenergia, Estrada do Paço do Lumiar 22, 1649-038 Lisboa, Portugal. Electronic address: teresa.lopessilva@lneg.pt.
authors.forename: Teresa Lopes
authors.initials: TL
authors.lastname: da Silva
mesh_terms: D001487:Basidiomycota / Q000378:metabolism*; D002244:Carbon / Q000378:metabolism*; D002338:Carotenoids / Q000096:biosynthesis*; D005434:Flow Cytometry; D008055:Lipids / Q000096:biosynthesis*
chemical_list: D008055:Lipids; D002338:Carotenoids; D002244:Carbon
keywords: Carob pulp syrup; Carotenoids; Flow cytometry; Lipids; Sugarcane molasses; Yeast