title: Structural and dynamical properties of reconstituted myelin sheaths in the presence of myelin proteins MBP and P2 studied by neutron scattering.
abstract: The myelin sheath is a tightly packed, multilayered membrane structure wrapped around selected nerve axons in the central and the peripheral nervous system. Because of its electrical insulation of the axons, which allows fast, saltatory nerve impulse conduction, myelin is crucial for the proper functioning of the vertebrate nervous system. A subset of myelin-specific proteins is well-defined, but their influence on membrane dynamics, i.e. myelin stability, has not yet been explored in detail. We investigated the structure and the dynamics of reconstituted myelin membranes on a pico- to nanosecond timescale, influenced by myelin basic protein (MBP) and myelin protein 2 (P2), using neutron diffraction and quasi-elastic neutron scattering. A model for the scattering function describing molecular lipid motions is suggested. Although dynamical properties are not affected significantly by MBP and P2 proteins, they act in a highly synergistic manner influencing the membrane structure.
authors.affiliation: University Joseph Fourier UFR PhITEM, Grenoble, France.
authors.forename: Wiebke
authors.initials: W
authors.lastname: Knoll
authors.forename: Judith
authors.initials: J
authors.lastname: Peters
authors.forename: Petri
authors.initials: P
authors.lastname: Kursula
authors.forename: Yuri
authors.initials: Y
authors.lastname: Gerelli
authors.forename: Jacques
authors.initials: J
authors.lastname: Ollivier
authors.forename: Bruno
authors.initials: B
authors.lastname: Demé
authors.forename: Mark
authors.initials: M
authors.lastname: Telling
authors.forename: Ewout
authors.initials: E
authors.lastname: Kemner
authors.forename: Francesca
authors.initials: F
authors.lastname: Natali
mesh_terms: D006801:Humans; D008051:Lipid Bilayers / Q000737:chemistry / Q000378:metabolism; D004676:Myelin Basic Protein / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D009186:Myelin Sheath / Q000737:chemistry*; D033363:Neutron Diffraction; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D053838:Scattering, Small Angle; D055672:Static Electricity; D013696:Temperature
chemical_list: D008051:Lipid Bilayers; D004676:Myelin Basic Protein; D011994:Recombinant Proteins