title: Five novel variants of GPR103 and their expression in different tissues of goose (Anser cygnoides).
abstract: GPR103 plays an important role in various tissues, while little information is available about the alternative splicing (AS) of its mRNA. In the present study, we used genomic PCR to identify the partial genomic locus of goose (Anser cygnoides) GPR103 and rapid amplification of cDNA ends (RACE)-PCR to identify five GPR103 variants, including the full-length variant (aGPR103-n) and four alternatively spliced variants (aGPR103-va, -vb, -vc and -vd). Sequence analysis showed that aGPR103-va and -vd are less likely to undergo nonsense-mediated mRNA decay, suggesting that they may be translated into truncated proteins. Quantitative real-time PCR (qRT-PCR) analysis revealed that the five variants are widely distributed in the brain and peripheral tissues of geese and show specific expression patterns. Thus, we here provide the first account of the GPR103 genomic locus and illustrate its transcriptional diversity and widespread distribution in geese.
authors.affiliation: Institute of Animal Breeding & Genetics, Sichuan Agricultural University, Ya'an, Sichuan 625014, PR China.
authors.forename: Qihai
authors.initials: Q
authors.lastname: Xiao
authors.affiliation: Institute of Animal Breeding & Genetics, Sichuan Agricultural University, Ya'an, Sichuan 625014, PR China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Liu
authors.affiliation: Institute of Animal Breeding & Genetics, Sichuan Agricultural University, Ya'an, Sichuan 625014, PR China.
authors.forename: Liang
authors.initials: L
authors.lastname: Li
authors.affiliation: Institute of Animal Breeding & Genetics, Sichuan Agricultural University, Ya'an, Sichuan 625014, PR China. Electronic address: wjw2886166@163.com.
authors.forename: Jiwen
authors.initials: J
authors.lastname: Wang
authors.affiliation: Institute of Animal Breeding & Genetics, Sichuan Agricultural University, Ya'an, Sichuan 625014, PR China.
authors.forename: Kailiang
authors.initials: K
authors.lastname: Huang
authors.affiliation: Institute of Animal Breeding & Genetics, Sichuan Agricultural University, Ya'an, Sichuan 625014, PR China.
authors.forename: Hehe
authors.initials: H
authors.lastname: Liu
authors.affiliation: Institute of Animal Breeding & Genetics, Sichuan Agricultural University, Ya'an, Sichuan 625014, PR China.
authors.forename: Chunchun
authors.initials: C
authors.lastname: Han
authors.affiliation: Institute of Animal Breeding & Genetics, Sichuan Agricultural University, Ya'an, Sichuan 625014, PR China.
authors.forename: Zhixiong
authors.initials: Z
authors.lastname: Pan
authors.affiliation: Institute of Animal Breeding & Genetics, Sichuan Agricultural University, Ya'an, Sichuan 625014, PR China.
authors.forename: Xia
authors.initials: X
authors.lastname: Dong
authors.affiliation: Institute of Animal Breeding & Genetics, Sichuan Agricultural University, Ya'an, Sichuan 625014, PR China.
authors.forename: Kai
authors.initials: K
authors.lastname: Lu
authors.affiliation: Institute of Animal Breeding & Genetics, Sichuan Agricultural University, Ya'an, Sichuan 625014, PR China.
authors.forename: Le
authors.initials: L
authors.lastname: Li
authors.affiliation: Institute of Animal Breeding & Genetics, Sichuan Agricultural University, Ya'an, Sichuan 625014, PR China.
authors.forename: Rongping
authors.initials: R
authors.lastname: Zhang
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D005777:Geese / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D014644:Genetic Variation; D009928:Organ Specificity; D010802:Phylogeny; D012333:RNA, Messenger / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000235:genetics* / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D043562:Receptors, G-Protein-Coupled
keywords: Alternative splicing; Expression pattern; GPR103; Goose