title: Syntaxin of plant proteins SYP123 and SYP132 mediate root hair tip growth in Arabidopsis thaliana.
abstract: Root hairs are fast-growing tubular protrusions on root epidermal cells that play important roles in water and nutrient uptake in plants. The tip-focused polarized growth of root hairs is accomplished by the secretion of newly synthesized materials to the tip via the polarized membrane trafficking mechanism. Here, we report the function of two different types of plasma membrane (PM) Qa-SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors), SYP123 and SYP132, in the growth of root hair in Arabidopsis. We found that SYP123, but not SYP132, localizes in the tip region of root hairs by recycling between the brefeldin A (BFA)-sensitive endosomes and the PM of the expanding tip in an F-actin-dependent manner. The vesicle-associated membrane proteins VAMP721/722/724 also exhibited tip-focused localization in root hairs and formed ternary SNARE complexes with both SYP123 and SYP132. These results demonstrate that SYP123 and SYP132 act in a coordinated fashion to mediate tip-focused membrane trafficking for root hair tip growth.
authors.affiliation: Department of Life and Environmental Sciences, Kyoto Prefectural University, Shimogamonakaragi-cho 1-5, Sakyo-ku, Kyoto, 606-8522 Japan.
authors.forename: Mie
authors.initials: M
authors.lastname: Ichikawa
authors.forename: Tomoko
authors.initials: T
authors.lastname: Hirano
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Enami
authors.forename: Taylor
authors.initials: T
authors.lastname: Fuselier
authors.forename: Naohiro
authors.initials: N
authors.lastname: Kato
authors.forename: Chian
authors.initials: C
authors.lastname: Kwon
authors.forename: Boris
authors.initials: B
authors.lastname: Voigt
authors.forename: Paul
authors.initials: P
authors.lastname: Schulze-Lefert
authors.forename: František
authors.initials: F
authors.lastname: Baluška
authors.forename: Masa H
authors.initials: MH
authors.lastname: Sato
mesh_terms: D000199:Actins / Q000378:metabolism; D017360:Arabidopsis / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D020126:Brefeldin A / Q000494:pharmacology; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000494:pharmacology; D036681:Fluorescence Recovery After Photobleaching; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008954:Models, Biological; D046912:Multiprotein Complexes / Q000378:metabolism; D009154:Mutation / Q000235:genetics; D010641:Phenotype; D018517:Plant Roots / Q000166:cytology / Q000187:drug effects / Q000254:growth & development*; D011485:Protein Binding / Q000187:drug effects; D021381:Protein Transport / Q000187:drug effects; D050765:Qa-SNARE Proteins / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D050600:SNARE Proteins / Q000378:metabolism; D053778:Thiazolidines / Q000494:pharmacology
chemical_list: D000199:Actins; D029681:Arabidopsis Proteins; D019086:Bridged Bicyclo Compounds, Heterocyclic; D046912:Multiprotein Complexes; D050765:Qa-SNARE Proteins; D011993:Recombinant Fusion Proteins; D050600:SNARE Proteins; C000593206:SYP123 protein, Arabidopsis; C568818:SYP132 protein, Arabidopsis; D053778:Thiazolidines; D049452:Green Fluorescent Proteins; D020126:Brefeldin A; C037068:latrunculin B
keywords: Arabidopsis; Cell polarity; Membrane traffic; Root hair cells; SNARE