title: Association of adiponectin +276G/T polymorphism with knee osteoarthritis.
abstract: Osteoarthritis (OA) is a prevalent, degenerative joint disorder leading to the destruction of articular cartilage, osteophyte formation and subchondral bone sclerosis. Genetic and environmental factors are involved in the development of OA. The role of adiponectin gene polymorphisms in OA has not yet been established. The aim of this study was to investigate the association of adiponectin +276G/T (rs1501299) gene polymorphism with knee OA. Genotype distributions and allelic frequencies of adiponectin gene, +276G/T polymorphism were determined in a total of 200 subjects (100 knee OA patients and 100 healthy controls). Single-nucleotide polymorphism (SNP) of the adiponectin +276G/T gene was genotyped by polymerase chain reaction (PCR)-restriction fragment length polymorphism analysis. The genotype distribution of +276G/T SNP was observed in the Hardy-Weinberg equilibrium for OA patients and controls. No statistically significant difference was identified between the two groups with respect to genotype distributions and allelic frequencies (P>0.05). The T- and G-allele frequencies were indicated as 24.5 and 75.5%, respectively, in OA patients, whereas the frequency was 23-70% in the control group. Findings of this study therefore suggest that the +276G/T SNP was not associated with susceptibility to knee OA.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: Dong
authors.initials: D
authors.lastname: Zhan
authors.affiliation: Department of Orthopaedics, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: Pongsak
authors.initials: P
authors.lastname: Yuktanandana
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: Wilai
authors.initials: W
authors.lastname: Anomasiri
authors.affiliation: Department of Orthopaedics, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: Aree
authors.initials: A
authors.lastname: Tanavalee
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand ; Department of Orthopaedics, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: Sittisak
authors.initials: S
authors.lastname: Honsawek
keywords: Thai population; adiponectin; case-control study; knee osteoarthritis; single-nucleotide polymorphism