title: Transcriptome expression profiling in response to drought stress in Paulownia australis.
abstract: The response and adaptation to drought remains poorly understood for Paulownia australis. To investigate this issue, transcriptome profiling of four P. australis accessions (two diploid and the other two autotetraploid) under water stress condition were studied using Illumina Genome Analyzer IIx analysis. The current study aimed to identify genes of P. australis metabolism pathways that might be involved in this plant's response to water deficit. Potted seedlings were subjected to well-watered conditions and drought stress, respectively. More than 290 million raw transcript reads were assembled into 111,660 unigenes, with a mean length of 1013 bp. Clusters of orthologous groups, gene ontology and the Kyoto Encyclopedia of Genes and Genomes annotations analyses were performed on the unigenes. Many differentially expressed genes and several metabolic pathways were identified. Quantitative real-time polymerase chain reaction was used to verify the expression patterns of 14 genes. Our study identified altered gene expression in P. australis induced by drought stress and provided a comprehensive map of drought-responsive genes and pathways in this species. To our knowledge, this is the first publicly available global transcriptome study of P. australis. This study provides a valuable genetic resource for this species.
authors.affiliation: Institute of Paulownia, Henan Agricultural University, 95 Wenhua Road, Jinshui Area, Zhengzhou 450002, Henan, China. dongdyp@163.com.
authors.forename: Yanpeng
authors.initials: Y
authors.lastname: Dong
authors.affiliation: Institute of Paulownia, Henan Agricultural University, 95 Wenhua Road, Jinshui Area, Zhengzhou 450002, Henan, China. zlxx64@126.com.
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Fan
authors.affiliation: Institute of Paulownia, Henan Agricultural University, 95 Wenhua Road, Jinshui Area, Zhengzhou 450002, Henan, China. zhaozl@126.com.
authors.forename: Zhenli
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: College of Forestry, Henan Agricultural University, 95 Wenhua Road, Jinshui Area, Zhengzhou 450002, Henan, China. dengmj1980@126.com.
authors.forename: Minjie
authors.initials: M
authors.lastname: Deng
mesh_terms: D000465:Algorithms; D004171:Diploidy; D055864:Droughts; D018506:Gene Expression Regulation, Plant; D063990:Gene Ontology; D019684:Magnoliopsida / Q000145:classification / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013045:Species Specificity; D013312:Stress, Physiological / Q000235:genetics; D057891:Tetraploidy; D059467:Transcriptome / Q000235:genetics*