title: In situ effects of urban river pollution on the mudsnail Potamopyrgus antipodarum as part of an integrated assessment.
abstract: The freshwater mudsnail (Potamopyrgus antipodarum) is sensitive to toxicity of both sediment and water and also to the endocrine disrupting compounds (EDC) at environmentally relevant concentrations. This study determined effects of in situ exposure of P. antipodarum as a part of a complex assessment of the impact of a city metropolitan area with large waste water treatment plant (WWTP) for 0.5 million population equivalents on two urban rivers. The study combined the in situ biotest with detailed chemical analyses and a battery of in vitro bioassays of both sediment and water. Passive sampling of river water was conducted during the course of exposure of the mudsnail. P. antipodarum was exposed for 8 weeks in cages permeable to sediment and water at localities up- and down-stream of the city of Brno, Czech Republic and downstream of the WWTP in two rivers. Greater mortality and significantly decreased embryo production of P. antipodarum were observed immediately downstream of the city of Brno. P. antipodarum exposed at locations downstream of the metropolitan area and WWTP exhibited greater mortality, while numbers of embryos produced by surviving individuals were comparable or slightly greater than for individuals held at the least polluted location. Comparisons with results of chemical analysis and in vitro assays indicate occurrence of groups of compounds contributing to observed effects. Differences in mortalities of mudsnails among sites corresponded well with in vitro cytotoxicity and concentrations of metals. The results of this study confirm the applicability of this novel field biotest with P. antipodarum for the evaluation of the effects of river pollution on metazoans, especially as suitable in situ part of integrative contamination assessment.
authors.affiliation: Masaryk University, Faculty of Science, RECETOX, Kamenice 753/5, 62500 Brno, Czech Republic.
authors.forename: Radka
authors.initials: R
authors.lastname: Zounkova
authors.affiliation: Masaryk University, Faculty of Science, RECETOX, Kamenice 753/5, 62500 Brno, Czech Republic.
authors.forename: Veronika
authors.initials: V
authors.lastname: Jalova
authors.affiliation: Masaryk University, Faculty of Science, RECETOX, Kamenice 753/5, 62500 Brno, Czech Republic.
authors.forename: Martina
authors.initials: M
authors.lastname: Janisova
authors.affiliation: Institute of Public Health, Partyzánské nám. 7, 70200 Ostrava, Czech Republic.
authors.forename: Tomas
authors.initials: T
authors.lastname: Ocelka
authors.affiliation: Institute of Public Health, Partyzánské nám. 7, 70200 Ostrava, Czech Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Jurcikova
authors.affiliation: Czech Hydrometeorological Institute, Kroftova 2578/43, 61600 Brno, Czech Republic.
authors.forename: Jarmila
authors.initials: J
authors.lastname: Halirova
authors.affiliation: Department Biomedical Veterinary Sciences and Toxicology Centre, University of Saskatchewan, 52 Campus Drive, Saskatoon, SK S7N 5B4 Saskatchewan, Canada; Department of Zoology, and Center for Integrative Toxicology, Michigan State University, East Lansing, MI, USA; Department of Biology and Chemistry and State Key Laboratory in Marine Pollution, City University of Hong Kong, Kowloon, Hong Kong Special Administrative Region; School of Biological Sciences, University of Hong Kong, Hong Kong Special Administrative Region; State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, People's Republic of China.
authors.forename: John P
authors.initials: JP
authors.lastname: Giesy
authors.affiliation: Masaryk University, Faculty of Science, RECETOX, Kamenice 753/5, 62500 Brno, Czech Republic. Electronic address: hilscherova@recetox.muni.cz.
authors.forename: Klara
authors.initials: K
authors.lastname: Hilscherova
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D004784:Environmental Monitoring; D019015:Geologic Sediments / Q000737:chemistry; D006801:Humans; D020128:Inhibitory Concentration 50; D009026:Mortality; D012098:Reproduction / Q000187:drug effects; D045483:Rivers / Q000737:chemistry; D012908:Snails / Q000187:drug effects*; D016019:Survival Analysis; D014505:Urban Population; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000633:toxicity*
chemical_list: D014874:Water Pollutants, Chemical
keywords: Gastropoda; Mortality; Passive sampling; Reproduction; Sediment, In vitro