title: Joint detection of multiple immunohistochemical indices and clinical significance in breast cancer.
abstract: Breast cancer is one of the most common malignancies in women. This study was conducted to analyze the association between the expressions of eight immunohistochemical (IHC) indices and clinicopathological characteristics in breast cancers (BCs) and investigate the clinical significance. IHC Envision ldpe-g-nvp was used to detect the expression of estrogen receptor (ER), progesterone receptor (PR), human epidermal growth factor receptor-2 (HER2), vascular endothelial growth factor (VEGF), epidermal growth factor receptor (EGFR), p53, type II topoisomerase (TOPO II) and Ki-67 in postoperative paraffin blocks of 286 cases of invasive BC and statistically analyzed their correlations with clinicopathological characteristics. The positive rates of ER, PR, HER2, VEGF, p53, EGFR, TOPO II and Ki-67 expression were 62.24, 41.96, 57.34, 53.85, 81.82, 46.85, 54.55 and 69.93%, respectively. ER expression was negatively correlated with age, tumor size and histological grade (P<0.05) and PR expression was negatively correlated with age and histological grade (P<0.05). Among the ER, PR and c-erbB-2 statuses, a significant correlation was observed between ER expression and PR status (P=0.0000), whereas the expression of ER and PR exhibited a negative correlation with HER2 status (P<0.05). We also demonstrated a significant correlation between EGFR expression and lymph node metastasis (P=0.0240), p53 expression and tumor size (P=0.0300), p53 and Ki-67 expression and histological grade (P<0.05) and the expressions of VEGF, EGFR, p53, TOPO II, Ki-67 and HER2 status (P<0.05). In addition, the Luminal B and HER2/neu subtypes exhibited a close correlation with age (P<0.01), while the HER2/neu and triple-negative subtypes were positively correlated with poor histological grade (P<0.05). In conclusion, there is a definite correlation between IHC indices and clinicopathological characteristics in BCs. Combined detection of these indices may be significant in the evaluation of biological behavior and prognosis of BC and thus in the diagnosis and comprehensive treatment of this disease.
authors.affiliation: Departments of General Surgery, Affiliated to Nanjing Medical University, Nanjing, Jiangsu 210009;
authors.forename: En-Qi
authors.initials: EQ
authors.lastname: Qiao
authors.affiliation: Radiotherapy, Affiliated to Nanjing Medical University, Nanjing, Jiangsu 210009;
authors.forename: Minghua
authors.initials: M
authors.lastname: Ji
authors.affiliation: Research Center for Clinical Oncology, Affiliated to Nanjing Medical University, Nanjing, Jiangsu 210009;
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Wu
authors.affiliation: Departments of General Surgery, Affiliated to Nanjing Medical University, Nanjing, Jiangsu 210009;
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Pathology, Jiangsu Cancer Hospital, Affiliated to Nanjing Medical University, Nanjing, Jiangsu 210009;
authors.forename: Xinyu
authors.initials: X
authors.lastname: Xu
authors.affiliation: Research Center for Clinical Oncology, Affiliated to Nanjing Medical University, Nanjing, Jiangsu 210009;
authors.forename: Rong
authors.initials: R
authors.lastname: Ma
authors.affiliation: Nanjing Medical University, Nanjing, Jiangsu 210029;
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Xuzhou Medical College, Xuzhou, Jiangsu 221000, P.R. China.
authors.forename: Yuejun
authors.initials: Y
authors.lastname: He
authors.affiliation: Xuzhou Medical College, Xuzhou, Jiangsu 221000, P.R. China.
authors.forename: Quanbin
authors.initials: Q
authors.lastname: Zha
authors.affiliation: Departments of General Surgery, Affiliated to Nanjing Medical University, Nanjing, Jiangsu 210009;
authors.forename: Xue
authors.initials: X
authors.lastname: Song
authors.affiliation: Departments of General Surgery, Affiliated to Nanjing Medical University, Nanjing, Jiangsu 210009;
authors.forename: Liwei
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Departments of General Surgery, Affiliated to Nanjing Medical University, Nanjing, Jiangsu 210009;
authors.forename: Ji-Hai
authors.initials: JH
authors.lastname: Tang
keywords: breast cancer subtype; breast carcinoma; clinicopathological characteristics; immunohistochemical index