title: The short term effects of resveratrol on ischemia-reperfusion injury in rat testis.
abstract: OBJECTIVE
  The purpose of this study was to identify changes taking place in the rat testis at the 24th hour of reperfusion following testicular torsion and to evaluate the effects of resveratrol (RSV), a powerful antioxidant, in preventing these changes using novel biochemical parameters and histopathology.
  
  
  METHODS
  Eighteen adult male rats were divided into three groups: Sham-operated (S), torsion/detorsion (T/D), and T/D+RSV groups. In the T/D group, testicular ischemia was achieved by rotating the left testis 720° clockwise for 4h. In the T/D+RSV group, 20mg/kg RSV was administered intraperitoneally 30 min before detorsion. All rats were sacrificed 24h after detorsion. Serum and tissue malondialdehyde (MDA) concentrations, ischemia modified albumin (IMA), total oxidative status (TOS), total antioxidant status (TAS), oxidative stress index (OSI), and histopathological damage score were analyzed.
  
  
  RESULTS
  Serum MDA, IMA, TOS, and OSI levels rose significantly in the T/D group. Serum MDA and IMA values were lower in the T/D+RES groups, but not significantly. OSI and TOS values were lower in the T/D+RES group, and the difference was significant. TAS values decreased significantly in the T/D group and rose in the T/D+RSV group, but not significantly. Ipsilateral tissue MDA values were significantly elevated in the T/D group and decreased in the T/D+RSV group, but not significantly. Apoptosis and histopathological damage increased significantly in the T/D group and decreased significantly in the T/D+RSV group. In the contralateral testis, apoptosis increased significantly in the T/D group. It decreased significantly in the T/D+RSV group.
  
  
  CONCLUSIONS
  Our findings show that RSV had a protective effect against oxidative damage induced with a testicular T/D model, especially at the antiapoptotic and histopathological level. OSI may be a good guide to the clinical status of testicular T/D.
authors.affiliation: Department of Histology and Embryology, Karadeniz Technical University, Faculty of Medicine, 61080, Trabzon, Turkey. Electronic address: esinyulug@gmail.com.
authors.forename: Esin
authors.initials: E
authors.lastname: Yuluğ
authors.affiliation: Department of Histology and Embryology, Karadeniz Technical University, Faculty of Medicine, 61080, Trabzon, Turkey.
authors.forename: Sibel
authors.initials: S
authors.lastname: Türedi
authors.affiliation: Department of Urology, Karadeniz Technical University, Faculty of Medicine, 61080, Trabzon, Turkey.
authors.forename: Ersagun
authors.initials: E
authors.lastname: Karagüzel
authors.affiliation: Department of Urology, Karadeniz Technical University, Faculty of Medicine, 61080, Trabzon, Turkey.
authors.forename: Omer
authors.initials: O
authors.lastname: Kutlu
authors.affiliation: Program of Medical Laboratory Techniques, Vocational School of Health Sciences, Karadeniz Technical University, 61080, Trabzon, Turkey; Department of Medical Biochemistry, Karadeniz Technical University, Faculty of Medicine, 61080, Trabzon, Turkey.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Menteşe
authors.affiliation: Department of Medical Biochemistry, Karadeniz Technical University, Faculty of Medicine, 61080, Trabzon, Turkey.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Alver
mesh_terms: D000418:Albumins / Q000032:analysis; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000627:therapeutic use*; D017209:Apoptosis / Q000187:drug effects; D004353:Drug Evaluation, Preclinical; D007274:Injections, Intraperitoneal; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000517:prevention & control*; D000077185:Resveratrol; D013086:Spermatic Cord Torsion / Q000150:complications / Q000188:drug therapy* / Q000601:surgery; D013094:Spermatozoa / Q000473:pathology; D013267:Stilbenes / Q000008:administration & dosage / Q000627:therapeutic use*; D013737:Testis / Q000098:blood supply* / Q000737:chemistry / Q000473:pathology
chemical_list: D000418:Albumins; D000975:Antioxidants; D013267:Stilbenes; D008315:Malondialdehyde; D000077185:Resveratrol
keywords: Ischemia-modified albumin; Rat; Resveratrol; Testicular torsion–detorsion