title: Enhanced production of fumigaclavine C by ultrasound stimulation in a two-stage culture of Aspergillus fumigatus CY018.
abstract: Stimulation by physical means including ultrasound is important to cell morphology and the product yield. In this work, the effect of ultrasound on the production of fumigaclavine C (FC), a conidiation-associated alkaloid with strong anti-inflammatory activity, was investigated in a newly developed two-stage culture of Aspergillus fumigatus CY018. The optimum ultrasonication conditions consisted of exposing cultures (at 12h of growth phase) to 10-min repeated irradiation (4 times) with a 24-h interval at the fixed power (500 W). Under this condition, FC production reached 118.09 mg/L, which was 89% higher than the control and much higher than previous reported values. Morphological analysis demonstrated that mycelia morphology from ultrasonication was in the form smaller and looser pellets as compared to that of the control. In addition, conidia that is closely related to FC biosynthesis were significantly increased after ultrasound stimulation, with 3 folds of that from the control.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, PR China.
authors.forename: Ling-Yun
authors.initials: LY
authors.lastname: Yao
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, PR China.
authors.forename: Yi-Xiang
authors.initials: YX
authors.lastname: Zhu
authors.affiliation: Institute of Functional Biomolecules, State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, PR China.
authors.forename: Rui-Hua
authors.initials: RH
authors.lastname: Jiao
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, PR China. Electronic address: luyanhua@ecust.edu.cn.
authors.forename: Yan-Hua
authors.initials: YH
authors.lastname: Lu
authors.affiliation: Institute of Functional Biomolecules, State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, PR China. Electronic address: rxtan@nju.edu.cn.
authors.forename: Ren-Xiang
authors.initials: RX
authors.lastname: Tan
mesh_terms: D001232:Aspergillus fumigatus / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D018533:Biomass; D018929:Cell Culture Techniques / Q000379:methods*; D004876:Ergot Alkaloids / Q000096:biosynthesis*; D026121:Indole Alkaloids; D007700:Kinetics; D013172:Spores, Fungal / Q000378:metabolism; D014465:Ultrasonics / Q000379:methods*
chemical_list: D004876:Ergot Alkaloids; D026121:Indole Alkaloids; C492995:fumigaclavine C
keywords: Conidiation; Fumigaclavine C; Low-intensity ultrasound; Morphology; Two-stage culture