title: [Ecoepidemiology of rickettsial infection in rodents, ectoparasites and humans in northeastern Antioquia, Colombia].
abstract: BACKGROUND
  Rickettsia spp. are tick, flea or lice-borne pathogenic bacterium, usually carried by rodents. In the last decade three outbreaks of rickettsial disease including fatalities, occurred in the provinces of Antioquia and Córdoba in northwestern Colombia.
  
  
  OBJECTIVE
  The purpose of this study was to perform an ecological and epidemiological description of the Rickettsia spp infection in the recently affected region of Colombia.
  
  
  METHODS
  Samples were obtained from 354 rodents and their parasites captured in the municipalities of Apartadó, Turbo and Necoclí. Likewise, 220 human sera were also collected, for detection of infection by Rickettsia spp.
  
  
  RESULTS
  Indirect immunofluorescence assay (IFA) revealed that 130 (43%) of the rodents and 53 (24%) of the humans produced antibodies to Rickettsia spp. Additionally, rickettsial DNA was amplified by PCR from 23 (6.8%) rodent liver samples using primers directed to the genus specific gltA gene. While gltA sequences from rodent samples exhibited a 98.7% similitude with R . prowazekii, a sequence amplified from larvae of Amblyomma sp exhibited identities of >99% similarity with R. tamurae .
  
  
  CONCLUSIONS
  These results demonstrate the presence of rickettsia in rodents, ectoparasites and humans throughout the municipalities studied.
authors.affiliation: Grupo de Investigación en Ciencias Veterinarias, Centauro, Universidad de Antioquia, Medellín, Colombia.
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Quintero
authors.affiliation: Grupo de Investigación en Ciencias Veterinarias, Centauro, Universidad de Antioquia, Medellín, Colombia.
authors.forename: Andrés Felipe
authors.initials: AF
authors.lastname: Londoño
authors.affiliation: Grupo de Inmunovirología, Universidad de Antioquia, Medellín, Colombia.
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Díaz
authors.affiliation: Instituto Colombiano de Medicina Tropical, Universidad CES, Medellín, Colombia.
authors.forename: Piedad
authors.initials: P
authors.lastname: Agudelo-Flórez
authors.affiliation: Instituto Colombiano de Medicina Tropical, Universidad CES, Medellín, Colombia.
authors.forename: Margarita
authors.initials: M
authors.lastname: Arboleda
authors.affiliation: Grupo de Investigación en Ciencias Veterinarias, Centauro, Universidad de Antioquia, Medellín, Colombia.
authors.forename: Juan David
authors.initials: JD
authors.lastname: Rodas
mesh_terms: D000328:Adult; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D001096:Arachnid Vectors / Q000382:microbiology*; D002648:Child; D003105:Colombia / Q000453:epidemiology; D004196:Disease Outbreaks; D004197:Disease Reservoirs / Q000469:parasitology*; D019353:Endemic Diseases; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D007814:Larva / Q000382:microbiology; D008099:Liver / Q000382:microbiology; D008297:Male; D008875:Middle Aged; D008925:Mites / Q000382:microbiology; D010802:Phylogeny; D012281:Rickettsia / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D012282:Rickettsia Infections / Q000097:blood / Q000453:epidemiology* / Q000635:transmission / Q000662:veterinary; D012377:Rodentia / Q000097:blood / Q000469:parasitology*; D016036:Seroepidemiologic Studies; D012959:Socioeconomic Factors; D013984:Tick Infestations / Q000453:epidemiology / Q000662:veterinary*; D013987:Ticks / Q000382:microbiology
chemical_list: D000907:Antibodies, Bacterial; D007074:Immunoglobulin G