title: Detection of anthocyanins/anthocyanidins in animal tissues.
abstract: Dietary polyphenols may contribute to the prevention of several degenerative diseases, including cancer. Anthocyanins have been shown to possess potential anticancer activity. The aim of this study was to determine anthocyanin bioavailability in lung tissue of mice fed a blueberry diet (5% w/w) for 10 days or a bolus dose (10 mg/mouse; po) of a native mixture of bilberry anthocyanidins. All five anthocyanidins present in the blueberry were detected in the lung tissue using improved methods. The effect of various solvents on the stability of anthocyanins and their recovery from the biomatrix was analyzed. Detection of anthocyanins and their metabolites was performed by UPLC and LC-MS. Although anthocyanins were not detected, cyanidin was detected by UPLC-PDA and other anthocyanidins were detected by LC-MS, following conversion to anthocyanidins and selective extraction in isoamyl alcohol. The results show that anthocyanins can be detected in lung tissue of blueberry-fed mice and thus are bioavailable beyond the gastrointestinal tract.
authors.affiliation: James Graham Brown Cancer Center, ‡Department of Medicine, and #Department of Pharmacology and Toxicology, University of Louisville , Louisville, Kentucky 40202, United States.
authors.forename: Farrukh
authors.initials: F
authors.lastname: Aqil
authors.forename: Manicka V
authors.initials: MV
authors.lastname: Vadhanam
authors.forename: Jeyaprakash
authors.initials: J
authors.lastname: Jeyabalan
authors.forename: Jian
authors.initials: J
authors.lastname: Cai
authors.forename: Inder P
authors.initials: IP
authors.lastname: Singh
authors.forename: Ramesh C
authors.initials: RC
authors.lastname: Gupta
mesh_terms: D000818:Animals; D000872:Anthocyanins / Q000032:analysis* / Q000378:metabolism; D029798:Blueberry Plants / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D005260:Female; D008168:Lung / Q000737:chemistry* / Q000378:metabolism; D013058:Mass Spectrometry; D051379:Mice; D008819:Mice, Nude; D010936:Plant Extracts / Q000032:analysis* / Q000378:metabolism
chemical_list: D000872:Anthocyanins; D010936:Plant Extracts
keywords: anthocyanidins; anthocyanins; bioavailability; blueberry diet; lung tissue