title: Paenibacillus humi sp. nov., isolated from peat-soil.
abstract: A Gram-positive, facultative-anaerobic, rod-shaped and endospore-forming bacterium designated as strain J30-4(T) was isolated from peat-soil collected in Russia. 16S rRNA gene sequence analysis revealed that strain belongs to the genus Paenibacillus, closely related to Paenibacillus terrigena A35(T) (98.1%) and Paenibacillus harenae B519(T) (95.5%). Chemotaxonomic analysis revealed that strain J30-4(T) possessed menaquinone MK-7 as predominant quinone and diphosphatidylglycerol, phosphatidylglycerol, phosphatidyl-ethanolamine, phosphatidyl-N-methylethanolamine and several unknown lipids were detected in the polar lipid profile. The cell wall peptidoglycan was of the A1γ type (meso-diaminopimelic acid). The major fatty acids were anteiso-C₁₅:₀ and iso-C₁₆:₀. The DNA G+C content of J30-4(T) was 55.5 mol%. Based on phenotypic, chemotaxonomic and phylogenetic data presented in this study, strain J30-4(T) (= KEMC 7302-014(T) = JCM 18166(T)) is indicative of a new Paenibacillus species, for which the name Paenibacillus humi sp. nov., is proposed.
authors.affiliation: Department of Bio-engineering, Division of Natural Science, Kyonggi University.
authors.forename: Hyun-Sook
authors.initials: HS
authors.lastname: Kim
authors.forename: Sang-Seob
authors.initials: SS
authors.lastname: Lee
mesh_terms: D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D020459:Genes, rRNA; D050356:Lipid Metabolism; D008969:Molecular Sequence Data; D056507:Paenibacillus / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism; D010641:Phenotype; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012426:Russia; D012988:Soil Microbiology; D013045:Species Specificity; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C062629:menaquinone 7