title: Vertebrate cone opsins enable sustained and highly sensitive rapid control of Gi/o signaling in anxiety circuitry.
abstract: G protein-coupled receptors (GPCRs) coupling to Gi/o signaling pathways are involved in the control of important physiological functions, which are difficult to investigate because of the limitation of tools to control the signaling pathway with precise kinetics and specificity. We established two vertebrate cone opsins, short- and long-wavelength opsin, for long-lasting and repetitive activation of Gi/o signaling pathways in vitro and in vivo. We demonstrate for both opsins the repetitive fast, membrane-delimited, ultra light-sensitive, and wavelength-dependent activation of the Gi/o pathway in HEK cells. We also show repetitive control of Gi/o pathway activation in 5-HT1A receptor domains in the dorsal raphe nucleus (DRN) in brain slices and in vivo, which is sufficient to modulate anxiety behavior in mice. Thus, vertebrate cone opsins represent a class of tools for understanding the role of Gi/o-coupled GPCRs in health and disease.
authors.affiliation: Department of General Zoology and Neurobiology, ND7/31, Ruhr-University Bochum, Universitätsstrasse 150, 44780 Bochum, Germany.
authors.forename: Olivia A
authors.initials: OA
authors.lastname: Masseck
authors.affiliation: Department of General Zoology and Neurobiology, ND7/31, Ruhr-University Bochum, Universitätsstrasse 150, 44780 Bochum, Germany.
authors.forename: Katharina
authors.initials: K
authors.lastname: Spoida
authors.affiliation: INSERM, U968, Sorbonne Universités, UPMC Univ Paris 06, UMR_S 968, CNRS, UMR_7210 Institut de la Vision, Paris, F-75012, France.
authors.forename: Deniz
authors.initials: D
authors.lastname: Dalkara
authors.affiliation: Department of General Zoology and Neurobiology, ND7/31, Ruhr-University Bochum, Universitätsstrasse 150, 44780 Bochum, Germany.
authors.forename: Takashi
authors.initials: T
authors.lastname: Maejima
authors.affiliation: Department of General Zoology and Neurobiology, ND7/31, Ruhr-University Bochum, Universitätsstrasse 150, 44780 Bochum, Germany.
authors.forename: Johanna M
authors.initials: JM
authors.lastname: Rubelowski
authors.affiliation: Department of General Zoology and Neurobiology, ND7/31, Ruhr-University Bochum, Universitätsstrasse 150, 44780 Bochum, Germany.
authors.forename: Lutz
authors.initials: L
authors.lastname: Wallhorn
authors.affiliation: Department of Neurosciences, School of Medicine, Case Western Reserve University, Cleveland, OH 44106, USA.
authors.forename: Evan S
authors.initials: ES
authors.lastname: Deneris
authors.affiliation: Department of General Zoology and Neurobiology, ND7/31, Ruhr-University Bochum, Universitätsstrasse 150, 44780 Bochum, Germany. Electronic address: sxh106@gmail.com.
authors.forename: Stefan
authors.initials: S
authors.lastname: Herlitze
mesh_terms: D000818:Animals; D001007:Anxiety / Q000378:metabolism / Q000503:physiopathology*; D001522:Behavior, Animal / Q000502:physiology; D055358:Cone Opsins / Q000378:metabolism*; D019206:GTP-Binding Protein alpha Subunits, Gi-Go / Q000378:metabolism; D006801:Humans; D008027:Light; D051379:Mice; D009474:Neurons / Q000378:metabolism; D044282:Receptor, Serotonin, 5-HT1A / Q000378:metabolism; D017949:Retinal Cone Photoreceptor Cells / Q000378:metabolism*; D017299:Rod Opsins / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology*
chemical_list: D055358:Cone Opsins; D017299:Rod Opsins; C095855:long-wavelength opsin; D044282:Receptor, Serotonin, 5-HT1A; D019206:GTP-Binding Protein alpha Subunits, Gi-Go