title: Applicability of preparative overpressured layer chromatography and direct bioautography in search of antibacterial chamomile compounds.
abstract: In situ sample preparation and preparative overpressured layer chromatography (OPLC) fractionation on a 0.5 mm thick adsorbent layer of chamomile flower methanol extract prepurified by conventional gravitation accelerated column chromatography were applied in searching for bioactive components. Sample cleanup in situ on the adsorbent layer subsequent to sample application was performed using mobile phase flow in the opposite direction (the input and output of the eluent was exchanged). The antibacterial effect of the fractions obtained from the stepwise gradient OPLC separation with the flow in the normal direction was evaluated by direct bioautography against two Gram-negative bacteria: the luminescence gene tagged plant pathogenic Pseudomonas syringae pv. maculicola, and the naturally luminescent marine bacterium Vibrio fischeri. The fractions having strong activity were analyzed by SPME-GC/MS and HPLC/MS/MS. Mainly essential oil components, coumarins, flavonoids, phenolic acids, and fatty acids were tentatively identified in the fractions.
authors.forename: Agnes M
authors.initials: AM
authors.lastname: Móricz
authors.forename: Péter G
authors.initials: PG
authors.lastname: Ott
authors.forename: Agnes
authors.initials: A
authors.lastname: Alberti
authors.forename: Andrea
authors.initials: A
authors.lastname: Böszörményi
authors.forename: Eva
authors.initials: E
authors.lastname: Lemberkovics
authors.forename: Eva
authors.initials: E
authors.lastname: Szoke
authors.forename: Agnes
authors.initials: A
authors.lastname: Kéry
authors.forename: Emil
authors.initials: E
authors.lastname: Mincsovics
mesh_terms: D000327:Adsorption; D048908:Aliivibrio fischeri / Q000187:drug effects; D000900:Anti-Bacterial Agents / Q000737:chemistry*; D020843:Chamomile / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D002855:Chromatography, Thin Layer / Q000379:methods*; D035264:Flowers / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D006090:Gram-Negative Bacteria / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D009822:Oils, Volatile / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry*; D044224:Pseudomonas syringae / Q000187:drug effects; D053719:Tandem Mass Spectrometry
chemical_list: D000900:Anti-Bacterial Agents; D009822:Oils, Volatile; D010936:Plant Extracts