title: Anti-inflammatory effects of extract from Haliotis discus hannai fermented with Cordyceps militaris mycelia in RAW264.7 macrophages through TRIF-dependent signaling pathway.
abstract: In this study, Haliotis discus hannai (H. discus hannai) fermentation was attempted with Cordyceps militaris (C. militaris) mycelia using a solid culture. We tried to ferment H. discus hannai to determine the optimal conditions fermentation with regards to its anti-inflammatory effects. The extracts of H. discus hannai fermented with C. militaris mycelia (HFCM-5) showed higher nitric oxide inhibitory effects than H. discus hannai and C. militaris alone. HFCM-5 also decreased pro-inflammatory cytokines, TNF-α and IL-6 in a dose-dependent manner. HFCM-5 did not affect the MyD88-dependent pathway, but decreased phosphorylation of IRF3 and STAT1 which are involved in TRIF-dependent pathway. Taken together, our results suggest that HFCM-5 exerts its anti-inflammatory effects via TRIF signaling pathway and could potentially be used as a functional food in the regulation of inflammation.
authors.affiliation: Department of Biotechnology, Konkuk University, Chungju 380-701, Republic of Korea.
authors.forename: Hong-Joo
authors.initials: HJ
authors.lastname: Joung
authors.affiliation: Department of Biotechnology, Konkuk University, Chungju 380-701, Republic of Korea.
authors.forename: Yon-Suk
authors.initials: YS
authors.lastname: Kim
authors.affiliation: Department of Biotechnology, Konkuk University, Chungju 380-701, Republic of Korea.
authors.forename: Jin-Woo
authors.initials: JW
authors.lastname: Hwang
authors.affiliation: Department of Biotechnology, Konkuk University, Chungju 380-701, Republic of Korea.
authors.forename: Young-Ki
authors.initials: YK
authors.lastname: Han
authors.affiliation: Department of Food & Biotechnology, Korea National University of Transportation, Chungju 380-702, Republic of Korea.
authors.forename: Jae-Hyun
authors.initials: JH
authors.lastname: Jeong
authors.affiliation: Industry-Academic Cooperation Foundation, Jeju National University, Jeju 690-756, Republic of Korea.
authors.forename: Jung-Suck
authors.initials: JS
authors.lastname: Lee
authors.affiliation: Nokyong Research Center, Konkuk University, Chungju 380-701, Republic of Korea.
authors.forename: Sang-Ho
authors.initials: SH
authors.lastname: Moon
authors.affiliation: Nokyong Research Center, Konkuk University, Chungju 380-701, Republic of Korea.
authors.forename: Byong-Tae
authors.initials: BT
authors.lastname: Jeon
authors.affiliation: Department of Biotechnology, Konkuk University, Chungju 380-701, Republic of Korea; Nokyong Research Center, Konkuk University, Chungju 380-701, Republic of Korea. Electronic address: parkpj@kku.ac.kr.
authors.forename: Pyo-Jam
authors.initials: PJ
authors.lastname: Park
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport / Q000502:physiology*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002457:Cell Extracts / Q000494:pharmacology*; D002470:Cell Survival; D032661:Cordyceps; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D005285:Fermentation; D005786:Gene Expression Regulation / Q000187:drug effects; D008264:Macrophages / Q000502:physiology*; D051379:Mice; D008974:Mollusca / Q000737:chemistry*; D025282:Mycelium / Q000502:physiology; D015398:Signal Transduction
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; D000893:Anti-Inflammatory Agents; D002457:Cell Extracts; D016207:Cytokines
keywords: Anti-inflammatory; Cordyceps militaris; Fermentation; Haliotis discus hannai