title: Differential induction of Pisum sativum defense signaling molecules in response to pea aphid infestation.
abstract: This study demonstrates the sequence of enhanced generation of signal molecules such as phytohormones, i.e. jasmonic acid (JA), ethylene (ET), salicylic acid (SA), and a relatively stable free radical, nitric oxide (NO), in response of Pisum sativum L. cv. Cysterski seedling leaves to the infestation of pea aphid Acyrthosiphon pisum (Harris) at a varied population size. In time from 0 to 96h after A. pisum infestation these signal molecules accumulated transiently. Moreover, the convergence of these signaling pathways occurred. JA and its methyl derivative MeJA reached the first maximum of generation at 24th hour of infestation. An increase in ET and NO generation was observed at 48th hour of infestation. The increase in SA, JA/MeJA and ET concentrations in aphid-infested leaves occurred from the 72nd to 96th hour. In parallel, an increase was demonstrated for the activities of enzymes engaged in the biosynthesis of SA, such as phenylalanine ammonia-lyase (PAL) and benzoic acid 2-hydroxylase (BA2H). Additionally, a considerable post-infestation accumulation of transcripts for PAL was observed. An increase in the activity of lipoxygenase (LOX), an important enzyme in the biosynthesis of JA was noted. This complex signaling network may contribute to the coordinated regulation of gene expression leading to specific defence responses.
authors.affiliation: Department of Plant Physiology, Poznań University of Life Sciences, Wołyńska 35, 60-637 Poznań, Poland; Department of Plant Physiology, Vinh University, Le Duan 182, Vinh City, Viet Nam.
authors.forename: Van Chung
authors.initials: VC
authors.lastname: Mai
authors.affiliation: Department of Chemistry, Poznań University of Life Sciences, Wojska Polskiego 75, 60-625 Poznań, Poland.
authors.forename: Kinga
authors.initials: K
authors.lastname: Drzewiecka
authors.affiliation: Institute of Plant Products Technology, Poznań University of Life Sciences, Wojska Polskiego 31, 60-624 Poznań, Poland.
authors.forename: Henryk
authors.initials: H
authors.lastname: Jeleń
authors.affiliation: Department of Biochemistry and Biotechnology, Poznań University of Life Sciences, Dojazd 11, 60-632 Poznań, Poland.
authors.forename: Dorota
authors.initials: D
authors.lastname: Narożna
authors.affiliation: Department of Ecophysiology, Faculty of Biology, Adam Mickiewicz University, Umultowska 89, 60-614 Poznań, Poland.
authors.forename: Renata
authors.initials: R
authors.lastname: Rucińska-Sobkowiak
authors.affiliation: Chair of Plant Physiology and Biotechnology, Nicolaus Copernicus University, Gagarina 9, 87-100 Toruń, Poland.
authors.forename: Jacek
authors.initials: J
authors.lastname: Kęsy
authors.affiliation: Department of Plant Physiology, Poznań University of Life Sciences, Wołyńska 35, 60-637 Poznań, Poland.
authors.forename: Jolanta
authors.initials: J
authors.lastname: Floryszak-Wieczorek
authors.affiliation: Department of Botany and Ecology, University of Zielona Góra, Prof. Szafrana 1, 65-516 Zielona Góra, Poland.
authors.forename: Beata
authors.initials: B
authors.lastname: Gabryś
authors.affiliation: Department of Plant Physiology, Poznań University of Life Sciences, Wołyńska 35, 60-637 Poznań, Poland. Electronic address: morkunas@jay.up.poznan.pl.
authors.forename: Iwona
authors.initials: I
authors.lastname: Morkunas
mesh_terms: D000818:Animals; D001042:Aphids / Q000502:physiology*; D003517:Cyclopentanes / Q000378:metabolism; D005030:Ethylenes / Q000378:metabolism; D005260:Female; D016166:Free Radical Scavengers / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D009569:Nitric Oxide / Q000235:genetics / Q000378:metabolism; D054883:Oxylipins / Q000378:metabolism; D018532:Pisum sativum / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism*; D010937:Plant Growth Regulators / Q000235:genetics* / Q000378:metabolism; D018515:Plant Leaves / Q000201:enzymology / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020156:Salicylic Acid / Q000378:metabolism; D036226:Seedlings / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D013997:Time Factors
chemical_list: D003517:Cyclopentanes; D005030:Ethylenes; D016166:Free Radical Scavengers; D054883:Oxylipins; D010937:Plant Growth Regulators; D009569:Nitric Oxide; C011006:jasmonic acid; C036216:ethylene; D020156:Salicylic Acid
keywords: Acyrthosiphon pisum; Ethylene; Jasmonic acid; Nitric oxide; Pisum sativum; Salicylic acid