title: Analysis of proline metabolic enzymes in Oryza sativa under NaCl stress.
abstract: The regulation of proline accumulation in seedlings of rice (Oryza sativa L. cv. Badami) was investigated. The increasing concentration of NaCl from 0.5 to 2.5% progressively increased the proline content in rice. Proline accumulation caused by NaCl was related to protein proteolysis, an increase in OAT, P5CS, PSCR activity, a decrease in PDH activity. The maximum increase in proline content was recorded at 2.5% NaCl concentration as compared to control and other concentrations of NaCl. The highest significant activity of proline synthesizing enzymes, Delta1-Pyrrolline-5-carboxylate synthetase, Delta1-Pyrrolline-5-carboxylate reductase and Ornithine-delta-aminotransferase with a lowest activity of proline hydrolysis enzymes; Proline dehydrogenase were also recorded at 2.5% salinity over control and other concentrations of NaCl with a in-significant increase in the activity of Delta1-Pyrrolline-5-carboxylate synthetase and Ornithine-delta-aminotransferase at 0.5 % concentration of NaCl over control. Externally the addition of 300mg MnCl2, 220 ml(-1) 1/2 strength Hoagland solution, having 1% NaCl, was seen to increase a 893.9% in proline content of this variety as compared to control.
authors.forename: D L
authors.initials: DL
authors.lastname: Bagdi
authors.forename: B P
authors.initials: BP
authors.lastname: Shaw
mesh_terms: D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D012275:Oryza / Q000187:drug effects* / Q000201:enzymology*; D010940:Plant Proteins / Q000378:metabolism*; D011392:Proline / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology*
chemical_list: D010940:Plant Proteins; D012965:Sodium Chloride; D011392:Proline