title: Fabricating three-dimensional carbohydrate hydrogel microarray for lectin-mediated bacterium capturing.
abstract: Herein, a three-dimensional carbohydrate modified polyacrylamide hydrogel microarray (3D carbohydrate hydrogel microarray) has been fabricated and employed as micro-reactor for capturing Escherichia coli (E. coli) by multivalent binding of concanavalin A (Con A) with O-antigen on the cellular surface of E. coli and immobilized monosaccharides on hydrogel spot, and the interactions of type 1 fimbriae of E. coli with immobilized monosaccharides. Because of the transparent performance of polyacrylamide hydrogel, the captured E. coli can be directly observed by a conventional microscope under a bright-field mode. The experimental result demonstrates that α-D-mannopyranoside (Man-α) modified hydrogel surface shows high efficiency of E. coli capturing. The 3D Man-α hydrogel microarray-based assay shows reasonable low detection limit (1.0×10(4) cells/mL) and large dynamic range (1.0×10(5) to 1.0×10(9)cells/mL) for detecting E. coli. In addition, bacterial adhesion inhibition assay has been demonstrated by the interactions of E. coli with ten saccharides, and satisfactory results have been obtained.
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun 130022, PR China; University of Chinese Academy of Sciences, Beijing 100039, PR China.
authors.forename: Xia
authors.initials: X
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun 130022, PR China; University of Chinese Academy of Sciences, Beijing 100039, PR China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Lei
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun 130022, PR China; University of Chinese Academy of Sciences, Beijing 100039, PR China.
authors.forename: Fuyao
authors.initials: F
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun 130022, PR China.
authors.forename: Dianjun
authors.initials: D
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun 130022, PR China. Electronic address: wangzx@ciac.ac.cn.
authors.forename: Zhenxin
authors.initials: Z
authors.lastname: Wang
mesh_terms: D001422:Bacterial Adhesion / Q000502:physiology; D019149:Bioreactors / Q000382:microbiology*; D002469:Cell Separation / Q000295:instrumentation; D004867:Equipment Design; D019544:Equipment Failure Analysis; D004926:Escherichia coli / Q000302:isolation & purification* / Q000502:physiology*; D020100:Hydrogels / Q000737:chemistry*; D037102:Lectins / Q000737:chemistry / Q000378:metabolism*; D046228:Microarray Analysis / Q000295:instrumentation*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation; D009005:Monosaccharides / Q000737:chemistry*; D066330:Printing, Three-Dimensional / Q000295:instrumentation
chemical_list: D020100:Hydrogels; D037102:Lectins; D009005:Monosaccharides
keywords: Bacterium capturing; Lectin; Polyacrylamide hydrogel; Saccharides; Three-dimensional microarray