title: Unraveling the variability and genetic structure of barker frog Physalaemus cuvieri (Leiuperinae) populations from different regions of Brazil.
abstract: The barker frog Physalaemus cuvieri is widely distributed in South America and is found in all regions of Brazil. Significant intraspecific morphological variation in this species has been reported. To determine the genetic structure of the natural Brazilian populations of P. cuvieri, 10 different populations geographically separated by 99.41 to 2936.75 km were evaluated using 10 polymorphic microsatellite loci. In addition, mitochondrial DNA data were analyzed to determine genetic distance between the populations. The genetic variation was found to be significant in most of the populations (HE ranged from 0.40 to 0.59, and allelic richness ranged from 2.07 to 3.54). An FST value of 0.27 indicated that high genetic structure was present among the P. cuvieri populations. STRUCTURE analyses grouped the 10 populations into nine clusters and indicated that only two of the populations were not genetically differentiated. The genetic distance calculated from the mitochondrial DNA data showed values <0.03 for seven of the populations.
authors.affiliation: Departamento de Biologia Estrutural e Funcional, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, SP, Brasil.
authors.forename: M
authors.initials: M
authors.lastname: Conte
authors.affiliation: Departamento de Biologia Estrutural e Funcional, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, SP, Brasil.
authors.forename: C P
authors.initials: CP
authors.lastname: Targueta
authors.affiliation: Polo Regional Centro Sul, Agência Paulista de Tecnologia dos Agronegócios, Piracicaba, SP, Brasil.
authors.forename: M I
authors.initials: MI
authors.lastname: Zucchi
authors.affiliation: Departamento de Biologia Vegetal, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, SP, Brasil.
authors.forename: A P
authors.initials: AP
authors.lastname: Souza
authors.affiliation: Departamento de Biologia Estrutural e Funcional, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, SP, Brasil.
authors.forename: S M
authors.initials: SM
authors.lastname: Recco-Pimentel
mesh_terms: D000818:Animals; D001001:Anura / Q000145:classification / Q000235:genetics*; D001938:Brazil; D004276:DNA, Satellite / Q000235:genetics; D014644:Genetic Variation; D058974:Phylogeography
chemical_list: D004276:DNA, Satellite