title: Physicochemical studies of the reaction of (99m)Tc with 5,5'-diethyl barbituric acid, adenine, d-glucose and thiobarbituric acid at different temperatures.
abstract: The reaction of (99m)Tc pertechnetate with 5,5'-diethyl barbituric acid, adenine, d-glucose and thiobarbituric acid at different temperatures was studied. The solvent effect on the electronic absorption spectra of the reactions was recorded. The reaction mixtures have been analyzed at different times using TLC and a radiodetctor to show the peaks at the plates. (99m)Tc pertechnetate is obtained from the Mo generators. It is difficult to separate the complexes in the solid state. The percentage of (99m)Tc involved in the complexes can be determined. Characterization of the (99m)Tc complexes as well as the determination of the extent of radiolabeling was done by thin layer chromatography using 0.9% NaCl solution as a solvent. The Rf value of (99m)TcO4(-) is (≈1). The solvatochromism for the reaction of (99m)Tc with d-glucose was mainly affected by solute permanent dipole-solvent permanent dipole interaction, the dipolar interaction for the reaction of (99m)Tc with of 5,5'-diethyl barbituric acid and for the reaction of (99m)Tc with adenine and thiobarbituric was solute-solvent hydrogen bonding.
authors.affiliation: Faculty of Science, Chemistry Department, Alexandria University, Alexandria, Egypt.
authors.forename: M S
authors.initials: MS
authors.lastname: Masoud
authors.affiliation: Faculty of Science, Chemistry Department, Ain Shams University, Cairo, Egypt.
authors.forename: M F
authors.initials: MF
authors.lastname: El-Shahat
authors.affiliation: Nuclear Medicine Department, The International Medical Center, Cairo, Egypt. Electronic address: elkholany73@yahoo.com.
authors.forename: A S
authors.initials: AS
authors.lastname: Elkholany
mesh_terms: D000225:Adenine / Q000737:chemistry*; D005947:Glucose / Q000737:chemistry*; D006358:Hot Temperature; D013667:Technetium / Q000737:chemistry*; D013858:Thiobarbiturates / Q000737:chemistry*
chemical_list: D013858:Thiobarbiturates; D013667:Technetium; D005947:Glucose; D000225:Adenine
keywords: (99m)Tc; Radiolabeling; TLC; UV Spectra