title: Bone marrow mesenchymal stromal cells and olfactory ensheathing cells transplantation after spinal cord injury--a morphological and functional comparison in rats.
abstract: Cell therapy for spinal cord injury (SCI) is a promising strategy for clinical application. Both bone marrow mesenchymal stromal cells (MSCs; also known as bone marrow-derived 'mesenchymal stem cells') and olfactory ensheathing cells (OECs) have demonstrated beneficial effects following transplantation in animal models of SCI. However, due to the large number of affecting parameters that determine the therapy success and the lack of methodological consensus, the comparison of different works is difficult. Therefore, we compared the effects of MSC and OEC transplants at early or delayed time after a spinal cord contusion injury in the rat. Functional outcomes for locomotion, sensory perception and electrophysiological responses were assessed. Moreover, the grafted cells survival and the amount of cavity and spared tissue were studied. The findings indicate that grafted cells survived until 7 days post-injection, but markedly disappeared in the following 2 weeks. Despite the low survival of the cells, MSC and OEC grafts provided tissue protection after early and delayed transplantation. Nevertheless, only acute MSC grafts improved locomotion recovery in treadmill condition and electrophysiological outcomes with respect to the other injured groups. These results, together with previous works, indicate that the MSC seem a better option than OEC for treatment of contusion injuries.
authors.affiliation: Department of Cell Biology, Physiology and Immunology, Group of Neuroplasticity and Regeneration, Institute of Neurosciences, Edif. M, Universitat Autònoma de Barcelona, Bellaterra, E-08193, Spain; Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), Spain.
authors.forename: Abel
authors.initials: A
authors.lastname: Torres-Espín
authors.forename: Elena
authors.initials: E
authors.lastname: Redondo-Castro
authors.forename: Joaquim
authors.initials: J
authors.lastname: Hernández
authors.forename: Xavier
authors.initials: X
authors.lastname: Navarro
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D004195:Disease Models, Animal; D063928:Ependymoglial Cells / Q000502:physiology / Q000637:transplantation*; D019054:Evoked Potentials, Motor / Q000502:physiology; D005260:Female; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D009043:Motor Activity / Q000502:physiology; D018482:Muscle, Skeletal / Q000503:physiopathology; D009431:Neural Conduction / Q000502:physiology; D009830:Olfactory Bulb / Q000166:cytology; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function / Q000502:physiology; D012018:Reflex / Q000502:physiology; D066191:Sensorimotor Cortex / Q000503:physiopathology; D013116:Spinal Cord / Q000473:pathology* / Q000503:physiopathology*; D013119:Spinal Cord Injuries / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D013697:Thermosensing / Q000502:physiology; D014110:Touch / Q000502:physiology
keywords: cell therapy; mesenchymal stromal cells; olfactory ensheathing cells; spinal cord injury; transplantation