title: Expression of EMSY, a novel BRCA2-link protein, is associated with lymph node metastasis and increased tumor size in breast carcinomas.
abstract: BACKGROUND
  The EMSY gene encodes a BRCA2-binding partner protein that represses the DNA repair function of BRCA2 in non-hereditary breast cancer. Although amplification of EMSY gene has been proposed to have prognostic value in breast cancer, no data have been available concerning EMSY tissue expression patterns and its associations with clinicopathological features.
  
  
  METHODS
  In the current study, we examined the expression and localization pattern of EMSY protein by immunohistochemistry and assessed its prognostic value in a well-characterized series of 116 unselected breast carcinomas with a mean follow up of 47 months using tissue microarray technique.
  
  
  RESULTS
  Immunohistochemical expression of EMSY protein was detected in 76% of primary breast tumors, localized in nuclear (18%), cytoplasmic (35%) or both cytoplasmic and nuclear sites (23%). Univariate analysis revealed a significant positive association between EMSY expression and lymph node metastasis (p value=0.045) and larger tumor size (p value=0.027), as well as a non-significant relation with increased risk of recurrence (p value=0.088), whereas no association with patients' survival (log rank test, p value=0.482), tumor grade or type was observed.
  
  
  CONCLUSIONS
  Herein, we demonstrated for the first time the immunostaining pattern of EMSY protein in breast tumors. Our data imply that EMSY protein may have impact on clinicipathological parameters and could be considered as a potential target for breast cancer treatment.
authors.affiliation: Oncopathology Research Center and Dep pathology, Faculty of medicine, Iran University of Medical Sciences, Tehran, Iran E-mail : zahra.madjd@yahoo.com, mojtabavi.n@iums.ac.ir.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Madjd
authors.forename: Mohammad Esmaeil
authors.initials: ME
authors.lastname: Akbari
authors.forename: Amir Hassan
authors.initials: AH
authors.lastname: Zarnani
authors.forename: Maryam
authors.initials: M
authors.lastname: Khayamzadeh
authors.forename: Elham
authors.initials: E
authors.lastname: Kalantari
authors.forename: Nazanin
authors.initials: N
authors.lastname: Mojtabavi
mesh_terms: D000328:Adult; D000368:Aged; D024682:BRCA2 Protein / Q000378:metabolism; D001943:Breast Neoplasms / Q000401:mortality / Q000473:pathology*; D005260:Female; D005784:Gene Amplification; D006801:Humans; D008198:Lymph Nodes / Q000473:pathology; D008207:Lymphatic Metastasis / Q000473:pathology*; D008875:Middle Aged; D060787:Neoplasm Grading; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000378:metabolism; D009364:Neoplasm Recurrence, Local / Q000473:pathology*; D009687:Nuclear Proteins / Q000096:biosynthesis* / Q000378:metabolism; D012097:Repressor Proteins / Q000096:biosynthesis* / Q000378:metabolism; D046888:Tissue Array Analysis
chemical_list: D024682:BRCA2 Protein; C551750:BRCA2 protein, human; C480344:EMSY protein, human; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D012097:Repressor Proteins