title: [Establishment of localization ultrathin section for cytopathic cells].
abstract: OBJECTIVE
  To establish a localization ultrathin section method through which target cytopathic cells could be sectioned in situ.
  
  
  METHODS
  Lab-Tek Chamber slide system (177402) was selected as resin embedding mould. Cells infected with Human adenovirus type 5 (Ad5) or A/HN/SWL3/ 2009 (H1N1) influenza virus were embedded in situ as models. Target cytopathic cells were exposed by trimming, sectioned and observed under transmission electron microscope (TEM).
  
  
  RESULTS
  Target cells could be sectioned in situ and virus particles could be found easily on sections.
  
  
  CONCLUSIONS
  A localization ultrathin sectioning method was established and this technique could be applied in virus detection in cytopathic cells to improve TEM detection efficiency.
authors.forename: Jing-Dong
authors.initials: JD
authors.lastname: Song
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Qu
authors.forename: Tao
authors.initials: T
authors.lastname: Hong
mesh_terms: D000258:Adenovirus Infections, Human / Q000473:pathology* / Q000821:virology*; D000260:Adenoviruses, Human / Q000502:physiology / Q000648:ultrastructure*; D002460:Cell Line; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000502:physiology / Q000648:ultrastructure*; D007251:Influenza, Human / Q000473:pathology / Q000821:virology*; D046529:Microscopy, Electron, Transmission; D008867:Microtomy / Q000379:methods*