title: Alt a 1 from Alternaria interacts with PR5 thaumatin-like proteins.
abstract: Alt a 1 is a protein found in Alternaria alternata spores related to virulence and pathogenicity and considered to be responsible for chronic asthma in children. We found that spores of Alternaria inoculated on the outer surface of kiwifruits did not develop hyphae. Nevertheless, the expression of Alt a 1 gene was upregulated, and the protein was detected in the pulp where it co-localized with kiwi PR5. Pull-down assays demonstrated experimentally that the two proteins interact in such a way that Alt a 1 inhibits the enzymatic activity of PR5. These results are relevant not only for plant defense, but also for human health as patients with chronic asthma could suffer from an allergic reaction when they eat fruit contaminated with Alternaria.
authors.affiliation: Centre for Plant Biotechnology and Genomics (UPM-INIA), Pozuelo de Alarcon, Madrid, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Gómez-Casado
authors.affiliation: Centre for Plant Biotechnology and Genomics (UPM-INIA), Pozuelo de Alarcon, Madrid, Spain.
authors.forename: Amaya
authors.initials: A
authors.lastname: Murua-García
authors.affiliation: Centre for Plant Biotechnology and Genomics (UPM-INIA), Pozuelo de Alarcon, Madrid, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Garrido-Arandia
authors.affiliation: Centre for Plant Biotechnology and Genomics (UPM-INIA), Pozuelo de Alarcon, Madrid, Spain.
authors.forename: Pablo
authors.initials: P
authors.lastname: González-Melendi
authors.affiliation: Centre for Plant Biotechnology and Genomics (UPM-INIA), Pozuelo de Alarcon, Madrid, Spain.
authors.forename: Rosa
authors.initials: R
authors.lastname: Sánchez-Monge
authors.affiliation: Institute of Applied Molecular Medicine (IMMA), CEU San Pablo University, Spain.
authors.forename: Domingo
authors.initials: D
authors.lastname: Barber
authors.affiliation: Centre for Plant Biotechnology and Genomics (UPM-INIA), Pozuelo de Alarcon, Madrid, Spain; Department of Biotechnology, ETSI Montes, Technical University Madrid, Spain.
authors.forename: Luis F
authors.initials: LF
authors.lastname: Pacios
authors.affiliation: Centre for Plant Biotechnology and Genomics (UPM-INIA), Pozuelo de Alarcon, Madrid, Spain. Electronic address: araceli.diaz@upm.es.
authors.forename: Araceli
authors.initials: A
authors.lastname: Díaz-Perales
mesh_terms: D029042:Actinidia / Q000201:enzymology / Q000382:microbiology*; D000485:Allergens / Q000378:metabolism; D000528:Alternaria / Q000235:genetics / Q000378:metabolism*; D005638:Fruit / Q000201:enzymology / Q000382:microbiology*; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D015966:Gene Expression Regulation, Fungal; D006801:Humans; D062105:Molecular Docking Simulation; D010935:Plant Diseases / Q000382:microbiology; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs; D025941:Protein Interaction Mapping; D015854:Up-Regulation
chemical_list: D000485:Allergens; D005656:Fungal Proteins; D010940:Plant Proteins; C003427:thaumatin protein, plant
keywords: Alt a 1; Alternaria; Competitive inhibitor; Kiwi; Kiwi-PR5; PR5-thaumatin-like protein