title: Corneal stroma demarcation line after standard and high-intensity collagen crosslinking determined with anterior segment optical coherence tomography.
abstract: OBJECTIVE
  To use anterior segment optical coherence tomography (AS-OCT) to compare corneal stroma demarcation line depth after corneal collagen crosslinking (CXL) with 2 treatment protocols.
  
  
  METHODS
  Vardinoyiannion Eye Institute of Crete, Faculty of Medicine, University of Crete, Heraklion, Crete, Greece.
  
  
  METHODS
  Prospective comparative interventional case series.
  
  
  METHODS
  Corneal collagen crosslinking was performed in all eyes using the same ultraviolet-A (UVA) irradiation device (CCL-365). Eyes were treated for 30 minutes with 3 mW/cm(2) according to the standard Dresden protocol (Group 1) or for 10 minutes with 9 mW/cm(2) of UVA irradiation intensity (Group 2). One month postoperatively, 2 independent observers measured the corneal stroma demarcation line using AS-OCT.
  
  
  RESULTS
  Sixteen patients (21 eyes) were enrolled. Group 1 comprised 7 patients (9 eyes) and Group 2, 9 patients (12 eyes). The mean corneal stroma demarcation line depth was 350.78 μm ± 49.34 (SD) (range 256.5 to 410 μm) in Group 1 and 288.46 ± 42.37 μm (range 238.5 to 353.5 μm) in Group 2; the corneal stroma demarcation line was statistically significantly deeper in Group 1 than in Group 2 (P=.0058, t test for unpaired data).
  
  
  CONCLUSIONS
  The corneal stroma demarcation line was significantly deeper after a 30-minute CXL treatment than after a 10-minute CXL procedure with high-intensity UVA irradiation.
authors.affiliation: From Vardinoyiannion Eye Institute of Crete (Kymionis, Tsoulnaras, Grentzelos, Plaka, Liakopoulos, Tsakalis, Pallikaris), Faculty of Medicine, University of Crete, Heraklion, Crete, and OKEBE (Mikropoulos), Thessaloniki, Greece; Department of Ophthalmology (Kymionis), Bascom Palmer Eye Institute, University of Miami, Miami, Florida, USA. Electronic address: kymionis@med.uoc.gr.
authors.forename: George D
authors.initials: GD
authors.lastname: Kymionis
authors.affiliation: From Vardinoyiannion Eye Institute of Crete (Kymionis, Tsoulnaras, Grentzelos, Plaka, Liakopoulos, Tsakalis, Pallikaris), Faculty of Medicine, University of Crete, Heraklion, Crete, and OKEBE (Mikropoulos), Thessaloniki, Greece; Department of Ophthalmology (Kymionis), Bascom Palmer Eye Institute, University of Miami, Miami, Florida, USA.
authors.forename: Konstantinos I
authors.initials: KI
authors.lastname: Tsoulnaras
authors.affiliation: From Vardinoyiannion Eye Institute of Crete (Kymionis, Tsoulnaras, Grentzelos, Plaka, Liakopoulos, Tsakalis, Pallikaris), Faculty of Medicine, University of Crete, Heraklion, Crete, and OKEBE (Mikropoulos), Thessaloniki, Greece; Department of Ophthalmology (Kymionis), Bascom Palmer Eye Institute, University of Miami, Miami, Florida, USA.
authors.forename: Michael A
authors.initials: MA
authors.lastname: Grentzelos
authors.affiliation: From Vardinoyiannion Eye Institute of Crete (Kymionis, Tsoulnaras, Grentzelos, Plaka, Liakopoulos, Tsakalis, Pallikaris), Faculty of Medicine, University of Crete, Heraklion, Crete, and OKEBE (Mikropoulos), Thessaloniki, Greece; Department of Ophthalmology (Kymionis), Bascom Palmer Eye Institute, University of Miami, Miami, Florida, USA.
authors.forename: Argyro D
authors.initials: AD
authors.lastname: Plaka
authors.affiliation: From Vardinoyiannion Eye Institute of Crete (Kymionis, Tsoulnaras, Grentzelos, Plaka, Liakopoulos, Tsakalis, Pallikaris), Faculty of Medicine, University of Crete, Heraklion, Crete, and OKEBE (Mikropoulos), Thessaloniki, Greece; Department of Ophthalmology (Kymionis), Bascom Palmer Eye Institute, University of Miami, Miami, Florida, USA.
authors.forename: Dimitrios G
authors.initials: DG
authors.lastname: Mikropoulos
authors.affiliation: From Vardinoyiannion Eye Institute of Crete (Kymionis, Tsoulnaras, Grentzelos, Plaka, Liakopoulos, Tsakalis, Pallikaris), Faculty of Medicine, University of Crete, Heraklion, Crete, and OKEBE (Mikropoulos), Thessaloniki, Greece; Department of Ophthalmology (Kymionis), Bascom Palmer Eye Institute, University of Miami, Miami, Florida, USA.
authors.forename: Dimitrios A
authors.initials: DA
authors.lastname: Liakopoulos
authors.affiliation: From Vardinoyiannion Eye Institute of Crete (Kymionis, Tsoulnaras, Grentzelos, Plaka, Liakopoulos, Tsakalis, Pallikaris), Faculty of Medicine, University of Crete, Heraklion, Crete, and OKEBE (Mikropoulos), Thessaloniki, Greece; Department of Ophthalmology (Kymionis), Bascom Palmer Eye Institute, University of Miami, Miami, Florida, USA.
authors.forename: Nikolaos G
authors.initials: NG
authors.lastname: Tsakalis
authors.affiliation: From Vardinoyiannion Eye Institute of Crete (Kymionis, Tsoulnaras, Grentzelos, Plaka, Liakopoulos, Tsakalis, Pallikaris), Faculty of Medicine, University of Crete, Heraklion, Crete, and OKEBE (Mikropoulos), Thessaloniki, Greece; Department of Ophthalmology (Kymionis), Bascom Palmer Eye Institute, University of Miami, Miami, Florida, USA.
authors.forename: Ioannis G
authors.initials: IG
authors.lastname: Pallikaris
mesh_terms: D000293:Adolescent; D003094:Collagen / Q000378:metabolism*; D063171:Corneal Pachymetry; D003319:Corneal Stroma / Q000378:metabolism / Q000473:pathology*; D003432:Cross-Linking Reagents / Q000627:therapeutic use*; D005260:Female; D006801:Humans; D007640:Keratoconus / Q000188:drug therapy* / Q000378:metabolism; D008297:Male; D017319:Photosensitizing Agents / Q000627:therapeutic use; D011446:Prospective Studies; D012256:Riboflavin / Q000627:therapeutic use; D041623:Tomography, Optical Coherence; D014466:Ultraviolet Rays; D055815:Young Adult
chemical_list: D003432:Cross-Linking Reagents; D017319:Photosensitizing Agents; D003094:Collagen; D012256:Riboflavin