title: Liposome-encapsulated hemoglobin accelerates bronchial healing after pneumonectomy in the rat with or without preoperative radiotherapy.
abstract: Liposome-encapsulated hemoglobin (LEH) has been reported to accelerate wound healing in the stomach and skin in an experimental setting. LEH was tested in bronchial anastomotic healing after radiation and pneumonectomy in the rat. Sprague-Dawley rats (n = 61) received preoperative radiation (20 Gy) to the chest and underwent left pneumonectomy with bronchial stump closure using the Sweet method 4 days later, when they were randomized to receive intravenous infusion of LEH with high O2 affinity (P50 O2  = 17 mm Hg, 10 mL/kg, n = 32) or saline (n = 29). Additional rats (n = 18) were treated in the same way without preoperative radiation. Bronchial anastomotic healing was evaluated 2 days after surgery by determining the bursting pressure and infiltration of neutrophils, monocytes, and macrophages. Bronchial bursting pressure was elevated in the rats receiving LEH both in the unirradiated group (LEH 212 ± 78 vs. saline 135 ± 63 mm Hg, P < 0.05) and in rats with preoperative radiation (LEH 162 ± 48 vs. saline 116 ± 56 mm Hg, P < 0.01). Moreover, the percentage of rats with bursting pressure <100 mm Hg tended to be smaller in the unirradiated group (LEH 1/9 [11.1%] vs. saline 4/9 [44.4%], NS) and was significantly reduced in irradiated animals (LEH 3/32 [9.4%] vs. saline 11/29 [38%], P < 0.05). There were no morphological differences except for macrophage infiltration to the anastomotic area, which was significantly prominent in the LEH-treated rats (P < 0.05) regardless of the presence or absence of preoperative irradiation (IR). The results suggest that LEH with high O2 affinity may improve mechanical strength and morphological findings in bronchial anastomosis in rats regardless of the presence or absence of preoperative IR. The irradiated rats later treated with LEH had equivalent or better bronchial healing than that of saline-treated naïve animals undergoing pneumonectomy alone.
authors.affiliation: Department of Thoracic Surgery, Tokai University School of Medicine, Isehara, Kanagawa, Japan.
authors.forename: Haruka
authors.initials: H
authors.lastname: Takeichi
authors.forename: Akira T
authors.initials: AT
authors.lastname: Kawaguchi
authors.forename: Chieko
authors.initials: C
authors.lastname: Murayama
authors.forename: Junki
authors.initials: J
authors.lastname: Koike
authors.forename: Masayuki
authors.initials: M
authors.lastname: Iwazaki
mesh_terms: D000818:Animals; D001802:Blood Substitutes / Q000494:pharmacology / Q000627:therapeutic use*; D016503:Drug Delivery Systems; D006454:Hemoglobins / Q000494:pharmacology / Q000627:therapeutic use*; D008081:Liposomes / Q000494:pharmacology / Q000627:therapeutic use*; D011013:Pneumonectomy; D057234:Preoperative Period; D011878:Radiotherapy; D051381:Rats; D017207:Rats, Sprague-Dawley; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D001802:Blood Substitutes; D006454:Hemoglobins; D008081:Liposomes
keywords: Bronchopleural fistula; Liposome-encapsulated hemoglobin; Wound healing