title: Caffeine and diphenyl diselenide improve long-term memory impaired in middle-aged rats.
abstract: The aim of the present study was to evaluate the effects of diphenyl diselenide (PhSe)2 supplemented diet (10ppm) associated to the administration of caffeine (15mg/kg; i.g.) for 30days on the novel object recognition memory in middle-aged rats. The present findings showed that (PhSe)2-supplemented diet enhanced short-term memory, but not long-term memory, of middle-aged rats in the novel object recognition task. The (PhSe)2 supplemented diet associated with caffeine administration improved long-term memory, but did not alter short-term memory, impaired in middle-aged rats. Daily caffeine administration to middle-aged rats had no effect on the memory tasks. Diet supplemented with (PhSe)2 plus caffeine administration increased the number of crossings and rearings reduced in middle-aged rats. Caffeine administration plus (PhSe)2 diets were effective in increasing the number of rearings and crossings, respectively, in middle-aged rats, [(3)H] glutamate uptake was reduced in hippocampal slices of rats from (PhSe)2 and caffeine plus (PhSe)2 groups. In addition, animals supplemented with (PhSe)2 showed an increase in the pCREB/CREB ratio whereas pAkt/Akt ratio was not modified. These results suggest that the effects of (PhSe)2 on the short-term memory may be related to its ability to decrease the uptake of glutamate, influencing the increase of CREB phosphorylation. (PhSe)2-supplemented diet associated to the administration of caffeine improved long-term memory impaired in middle-aged rats, an effect independent of CREB and Akt phosphorylation.
authors.affiliation: Laboratório de Síntese, Reatividade e Avaliação Farmacológica e Toxicológica de Organocalcogênios, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
authors.forename: Marlon R
authors.initials: MR
authors.lastname: Leite
authors.affiliation: Laboratório de Síntese, Reatividade e Avaliação Farmacológica e Toxicológica de Organocalcogênios, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
authors.forename: Marcel Henrique
authors.initials: MH
authors.lastname: Marcondes Sari
authors.affiliation: Departamento de Análises Clínicas e Toxicológicas, Centro de Ciências da Saúde, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
authors.forename: Mayara L
authors.initials: ML
authors.lastname: de Freitas
authors.affiliation: Departamento de Análises Clínicas e Toxicológicas, Centro de Ciências da Saúde, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
authors.forename: Lia P
authors.initials: LP
authors.lastname: Oliveira
authors.affiliation: Departamento de Análises Clínicas e Toxicológicas, Centro de Ciências da Saúde, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
authors.forename: Laíza
authors.initials: L
authors.lastname: Dalmolin
authors.affiliation: Departamento de Análises Clínicas e Toxicológicas, Centro de Ciências da Saúde, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Brandão
authors.affiliation: Laboratório de Síntese, Reatividade e Avaliação Farmacológica e Toxicológica de Organocalcogênios, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil. Electronic address: gzeni@quimica.ufsm.br.
authors.forename: Gilson
authors.initials: G
authors.lastname: Zeni
mesh_terms: D000375:Aging / Q000523:psychology; D000818:Animals; D001555:Benzene Derivatives / Q000494:pharmacology / Q000627:therapeutic use*; D050882:CREB-Binding Protein / Q000378:metabolism; D002110:Caffeine / Q000494:pharmacology / Q000627:therapeutic use*; D019587:Dietary Supplements; D004353:Drug Evaluation, Preclinical / Q000379:methods; D018698:Glutamic Acid / Q000378:metabolism; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008569:Memory Disorders / Q000188:drug therapy* / Q000378:metabolism / Q000523:psychology; D057567:Memory, Long-Term / Q000187:drug effects; D008570:Memory, Short-Term / Q000187:drug effects; D016566:Organoselenium Compounds / Q000494:pharmacology / Q000627:therapeutic use*; D010766:Phosphorylation / Q000187:drug effects; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D021641:Recognition, Psychology / Q000187:drug effects; D046509:Tissue Culture Techniques
chemical_list: D001555:Benzene Derivatives; D016566:Organoselenium Compounds; C061132:diphenyldiselenide; D002110:Caffeine; D018698:Glutamic Acid; D050882:CREB-Binding Protein; C494365:Crebbp protein, rat; D051057:Proto-Oncogene Proteins c-akt
keywords: Caffeine; Memory; Middle-aged; Object recognition; Organoselenium