title: Circulating MicroRNAs in blood of patients with prostate cancer.
abstract: BACKGROUND
  MicroRNAs (miRNAs) are small regulatory RNAs that do not code for proteins. Detection of circulating tumor cells (CTC) would provide diagnostic and prognostic information in prostate tumors (PT). Thus, miRNAs could constitute a promising new class of biomarkers for CTC detection.
  
  
  OBJECTIVE
  To analyze circulating microRNAs in whole blood as non-invasive markers in patients with localized prostate cancer and healthy individuals.
  
  
  METHODS
  A preliminary study including a population of 40 patients with mean age of 71 years and mean PSA of 18, 9 ng/ml (range). Regarding the risk group (RG): 33.3% had low risk, 30% intermediate risk and 36.7% high risk. A previous in silico study identified 92 candidates and was followed by another in vivo to verify the findings of the former using array technology by real-time PCR.
  
  
  RESULTS
  Statistical analysis of the results revealed 10 microRNAs candidates with statistically significant differential expression between the different risk groups and healthy controls: hsa-miR-337-3p, hsa-miR-330-3p, hsa-miR-339-3p, hsa-miR-124, hsa-miR-218, hsa-miR-128, hsa-miR-10a, hsa-miR-199b-5p, hsa-miR-200b and hsa-miR-15b.
  
  
  CONCLUSIONS
  Our data suggest that circulating microRNAs can act as biomarkers to identify risk groups in CaP.
authors.affiliation: Instituto de Investigación Biomédica, A Coruña, INIBIC, CHU A Coruña-XXIAC, A Coruña, España. Electronic address: Vanessa.medina.villaamil@sergas.es.
authors.forename: V
authors.initials: V
authors.lastname: Medina-Villaamil
authors.affiliation: Servicio de Urología Médica, CHU A Coruña-XXIAC, A Coruña, España.
authors.forename: S
authors.initials: S
authors.lastname: Martínez-Breijo
authors.affiliation: Servicio de Urología Médica, CHU A Coruña-XXIAC, A Coruña, España.
authors.forename: P
authors.initials: P
authors.lastname: Portela-Pereira
authors.affiliation: Servicio de Oncología Médica, CHU A Coruña-XXIAC, A Coruña, España.
authors.forename: M
authors.initials: M
authors.lastname: Quindós-Varela
authors.affiliation: Instituto de Investigación Biomédica, A Coruña, INIBIC, CHU A Coruña-XXIAC, A Coruña, España.
authors.forename: I
authors.initials: I
authors.lastname: Santamarina-Caínzos
authors.affiliation: Servicio de Oncología Médica, CHU A Coruña-XXIAC, A Coruña, España; Departamento de Medicina, Universidade da Coruña, UDC, A Coruña, España.
authors.forename: L M
authors.initials: LM
authors.lastname: Antón-Aparicio
authors.affiliation: Servicio de Urología Médica, CHU A Coruña-XXIAC, A Coruña, España.
authors.forename: F
authors.initials: F
authors.lastname: Gómez-Veiga
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics; D008875:Middle Aged; D011471:Prostatic Neoplasms / Q000097:blood* / Q000235:genetics
chemical_list: D035683:MicroRNAs
keywords: Cáncer de próstata; MicroARN; MicroRNAs; Micrometastasis; Micrometástasis; Polymerase chain reaction; Prostate cancer; Reacción en cadena de la polimerasa