title: Trypanocidal action of tea tree oil (Melaleuca alternifolia) against Trypanosoma evansi in vitro and in vivo used mice as experimental model.
abstract: This study aimed to evaluate the Trypanosoma evansi susceptibility to tea tree oil (TTO - Melaleuca alternifolia) and tea tree oil nanocapsules (TTO nanocapsules) in vitro and in vivo tests. In vitro, we observed a mortality curve of trypomastigotes proportional to dose, i.e., the TTO and TTO nanocapsules have trypanocidal effect. Treatment with TTO in vivo was assessed in experiments (I and II). For Experiment I, T. evansi infected mice were treated with TTO and/or combinations of essential oil with chemotherapy (diminazene aceturate - D.A.). Treatment with TTO at a dose of 1mLkg(-1) was able to extend animal longevity, but had no curative efficacy. However, when TTO was combined with D.A. a disease curative efficacy of 100% for disease was observed, a much better result than the D.A. treatment (33.3%). In Experiment II, T. evansi infected mice were treated with TTO nanocapsules with doses of 0.3, 0.6 and 0.9mLkg(-1). Animals treated with 0.9mLkg(-1) showed higher longevity however without curative effect. Active compounds present in natural products, such as M. alternifolia, may potentiate the treatment of trypanosomosis when associated with other trypanocidal drugs.
authors.affiliation: Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil; Laboratory of Microbiology, Centro Universitário Franciscano, Santa Maria, RS, Brazil.
authors.forename: Matheus D
authors.initials: MD
authors.lastname: Baldissera
authors.affiliation: Department of Animal Science, Universidade do Estado de Santa Catarina (UDESC), Chapecó, SC, Brazil. Electronic address: aleksandro_ss@yahoo.com.br.
authors.forename: Aleksandro S
authors.initials: AS
authors.lastname: Da Silva
authors.affiliation: Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil.
authors.forename: Camila B
authors.initials: CB
authors.lastname: Oliveira
authors.affiliation: Laboratory of Microbiology, Centro Universitário Franciscano, Santa Maria, RS, Brazil; Laboratory of Nanotechnology, Centro Universitário Franciscano, Santa Maria, RS, Brazil.
authors.forename: Roberto C V
authors.initials: RC
authors.lastname: Santos
authors.affiliation: Laboratory of Microbiology, Centro Universitário Franciscano, Santa Maria, RS, Brazil.
authors.forename: Rodrigo A
authors.initials: RA
authors.lastname: Vaucher
authors.affiliation: Laboratory of Nanotechnology, Centro Universitário Franciscano, Santa Maria, RS, Brazil.
authors.forename: Renata P
authors.initials: RP
authors.lastname: Raffin
authors.affiliation: Laboratory of Nanotechnology, Centro Universitário Franciscano, Santa Maria, RS, Brazil.
authors.forename: Patrícia
authors.initials: P
authors.lastname: Gomes
authors.affiliation: Department of Animal Production, Universidade do Estado de Santa Catarina (UDESC), Lages, SC, Brazil.
authors.forename: Maria G C
authors.initials: MG
authors.lastname: Dambros
authors.affiliation: Department of Animal Production, Universidade do Estado de Santa Catarina (UDESC), Lages, SC, Brazil.
authors.forename: Luiz C
authors.initials: LC
authors.lastname: Miletti
authors.affiliation: Laboratory of Phytochemistry, UFSM, Santa Maria, RS, Brazil.
authors.forename: Aline A
authors.initials: AA
authors.lastname: Boligon
authors.affiliation: Laboratory of Phytochemistry, UFSM, Santa Maria, RS, Brazil.
authors.forename: Margareth L
authors.initials: ML
authors.lastname: Athayde
authors.affiliation: Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil. Electronic address: sgmonteiro@uol.com.br.
authors.forename: Silvia G
authors.initials: SG
authors.lastname: Monteiro
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D008297:Male; D051379:Mice; D053769:Nanocapsules; D051381:Rats; D020947:Tea Tree Oil / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D014344:Trypanocidal Agents / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D014345:Trypanosoma / Q000187:drug effects*; D014352:Trypanosomiasis / Q000188:drug therapy*
chemical_list: D053769:Nanocapsules; D014344:Trypanocidal Agents; D020947:Tea Tree Oil
keywords: TTO; Trypanocidal effect; Trypanosoma evansi