title: Investigation of the prevalence of antibody immunodeficiency in a polio endemic area in India.
abstract: BACKGROUND
  Protection against paralytic poliomyelitis is provided mainly by antibody mediated host defense. Despite intensive oral polio vaccine (OPV) immunization campaigns wild poliovirus transmission could not be stopped in Uttar Pradesh (UP) and Bihar states of India by the end of 2010. The objective of our study was to quantitate serum IgG and IgA in children of western UP, India, to determine the prevalence of antibody immunodeficiency.
  
  
  METHODS
  A cross-sectional survey for IgG and IgA concentrations in serum samples from healthy children and children with acute flaccid paralysis (AFP), up to the age of 5 years, was performed using sandwich ELISA.
  
  
  RESULTS
  The overall mean IgG concentration for 1882 children of western UP, India, was 10.57 ± 4.53 (SD) g/L and mean IgA concentration for 979 children was 1.2 ± 0.818 g/L. Two 7-month-old female children had IgG levels below 2 g/L and there was an absence of neutralizing polio antibodies. The mean serum IgG level of children with AFP (n=979) was lower than levels observed in healthy children (n=903). The proportion of children with IgG levels below 2 g/L and IgA levels below 0.07 g/L was 0.7% in both healthy children and AFP cases.
  
  
  CONCLUSIONS
  There was no abnormal prevalence of immunodeficiency in children in western UP which could have delayed achieving the eradication of polio in the state. The immunoglobulin levels reported here may be used as age-specific normal values for Indian children.
authors.affiliation: Enterovirus Research Centre, Indian Council of Medical Research, Haffkine Institute Campus, Acharya Donde Marg, Parel, Mumbai 400012, India.
authors.forename: Madhu C
authors.initials: MC
authors.lastname: Mohanty
authors.forename: Jagadish M
authors.initials: JM
authors.lastname: Deshpande
mesh_terms: D002675:Child, Preschool; D003140:Communicable Disease Control / Q000458:organization & administration*; D003430:Cross-Sectional Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D017589:Immunization Programs / Q000458:organization & administration*; D007070:Immunoglobulin A / Q000097:blood*; D007074:Immunoglobulin G / Q000097:blood*; D007194:India / Q000453:epidemiology; D007223:Infant; D008297:Male; D011051:Poliomyelitis / Q000453:epidemiology / Q000276:immunology* / Q000517:prevention & control; D017955:Poliovirus / Q000276:immunology*; D011055:Poliovirus Vaccine, Oral; D011159:Population Surveillance; D015995:Prevalence
chemical_list: D007070:Immunoglobulin A; D007074:Immunoglobulin G; D011055:Poliovirus Vaccine, Oral
keywords: Acute flaccid paralysis (AFP); Children; IgA; IgG; Poliomyelitis; Serum immunoglobulin