title: New insights on plant cell elongation: a role for acetylcholine.
abstract: We investigated the effect of auxin and acetylcholine on the expression of the tomato expansin gene LeEXPA2, a specific expansin gene expressed in elongating tomato hypocotyl segments. Since auxin interferes with clathrin-mediated endocytosis, in order to regulate cellular and developmental responses we produced protoplasts from tomato elongating hypocotyls and followed the endocytotic marker, FM4-64, internalization in response to treatments. Tomato protoplasts were observed during auxin and acetylcholine treatments after transient expression of chimerical markers of volume-control related compartments such as vacuoles. Here we describe the contribution of auxin and acetylcholine to LeEXPA2 expression regulation and we support the hypothesis that a possible subcellular target of acetylcholine signal is the vesicular transport, shedding some light on the characterization of this small molecule as local mediator in the plant physiological response.
authors.affiliation: DiSTeBA (Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali), University of Salento, Campus ECOTEKNE, Lecce 73100, Italy. gp.disansebastiano@unisalento.it.
authors.forename: Gian-Pietro
authors.initials: GP
authors.lastname: Di Sansebastiano
authors.affiliation: Interdepartmental Research Centre Biogest-Siteia, University of Modena and Reggio Emilia, Reggio Emilia 42122, Italy. silvia.fornaciari@unimore.it.
authors.forename: Silvia
authors.initials: S
authors.lastname: Fornaciari
authors.affiliation: Department of Life Science, University of Modena and Reggio Emilia, Reggio Emilia 42122, Italy. barozzi.fabrizio@virgilio.it.
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Barozzi
authors.affiliation: DiSTeBA (Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali), University of Salento, Campus ECOTEKNE, Lecce 73100, Italy. gabriella.piro@unisalento.it.
authors.forename: Gabriella
authors.initials: G
authors.lastname: Piro
authors.affiliation: Interdepartmental Research Centre Biogest-Siteia, University of Modena and Reggio Emilia, Reggio Emilia 42122, Italy. laura.arru@unimore.it.
authors.forename: Laura
authors.initials: L
authors.lastname: Arru
mesh_terms: D015084:2,4-Dichlorophenoxyacetic Acid / Q000494:pharmacology; D000109:Acetylcholine / Q000494:pharmacology*; D048429:Cell Size / Q000187:drug effects*; D004705:Endocytosis / Q000187:drug effects; D004721:Endoplasmic Reticulum / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D018546:Hypocotyl / Q000166:cytology / Q000187:drug effects / Q000235:genetics; D007210:Indoleacetic Acids / Q000494:pharmacology; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D018551:Solanum lycopersicum / Q000166:cytology / Q000187:drug effects* / Q000235:genetics; D018613:Microscopy, Confocal; D059828:Plant Cells / Q000187:drug effects* / Q000378:metabolism; D010937:Plant Growth Regulators / Q000494:pharmacology; D010940:Plant Proteins / Q000235:genetics; D021381:Protein Transport / Q000187:drug effects; D011523:Protoplasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D011726:Pyridinium Compounds / Q000378:metabolism; D000644:Quaternary Ammonium Compounds / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014617:Vacuoles / Q000378:metabolism
chemical_list: C092350:FM 4-64; D007210:Indoleacetic Acids; D008164:Luminescent Proteins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D011726:Pyridinium Compounds; D000644:Quaternary Ammonium Compounds; C095825:expansin protein, plant; D015084:2,4-Dichlorophenoxyacetic Acid; D000109:Acetylcholine