title: Enhanced expression of Bacillus subtilis yaaA can restore both the growth and the sporulation defects caused by mutation of rplB, encoding ribosomal protein L2.
abstract: A temperature-sensitive mutation in rplB, designated rplB142, encodes a missense mutation at position 142 [His (CAT) to Leu (CTT)] of Bacillus subtilis ribosomal protein L2. The strain carrying the mutation grew more slowly than the wild-type, even at low temperatures, probably due to the formation of defective 70S ribosomes and the accumulation of incomplete 50S subunits (50S* subunits). Gel analysis indicated that amounts of L2 protein and also of L16 protein were reduced in ribosomes prepared from the rplB142 mutant 90 min after increasing the growth temperature to 45 °C. These results suggest that the assembly of the L16 protein into the 50S subunit requires the native L2 protein. The H142L mutation in the defective L2 protein affected sporulation as well as growth, even at the permissive temperature. A suppressor mutation that restored both growth and sporulation of the rplB142 mutant at low temperature was identified as a single base deletion located immediately upstream of the yaaA gene that resulted in an increase in its transcription. Furthermore, genetic analysis showed that enhanced synthesis of YaaA restores the functionality of L2 (H142L) by facilitating its assembly into 50S subunits.
authors.affiliation: Department of Life Science and Research Center for Life Science, College of Science, Rikkyo University, Toshima-ku, Nishi-ikebukuro 3-34-1, Tokyo 171-8501, Japan.
authors.forename: Shota
authors.initials: S
authors.lastname: Suzuki
authors.affiliation: Department of Life Science and Research Center for Life Science, College of Science, Rikkyo University, Toshima-ku, Nishi-ikebukuro 3-34-1, Tokyo 171-8501, Japan.
authors.forename: Osamu
authors.initials: O
authors.lastname: Tanigawa
authors.affiliation: Department of Life Science and Research Center for Life Science, College of Science, Rikkyo University, Toshima-ku, Nishi-ikebukuro 3-34-1, Tokyo 171-8501, Japan.
authors.forename: Genki
authors.initials: G
authors.lastname: Akanuma
authors.affiliation: Department of Life Science and Research Center for Life Science, College of Science, Rikkyo University, Toshima-ku, Nishi-ikebukuro 3-34-1, Tokyo 171-8501, Japan.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Nanamiya
authors.affiliation: Department of Life Science and Research Center for Life Science, College of Science, Rikkyo University, Toshima-ku, Nishi-ikebukuro 3-34-1, Tokyo 171-8501, Japan.
authors.forename: Fujio
authors.initials: F
authors.lastname: Kawamura
authors.affiliation: Department of Life Science and Research Center for Life Science, College of Science, Rikkyo University, Toshima-ku, Nishi-ikebukuro 3-34-1, Tokyo 171-8501, Japan.
authors.forename: Kazumi
authors.initials: K
authors.lastname: Tagami
authors.affiliation: Department of Life Science and Research Center for Life Science, College of Science, Rikkyo University, Toshima-ku, Nishi-ikebukuro 3-34-1, Tokyo 171-8501, Japan.
authors.forename: Naofumi
authors.initials: N
authors.lastname: Nomura
authors.affiliation: Department of Life Science and Research Center for Life Science, College of Science, Rikkyo University, Toshima-ku, Nishi-ikebukuro 3-34-1, Tokyo 171-8501, Japan.
authors.forename: Teppei
authors.initials: T
authors.lastname: Kawabata
authors.affiliation: Department of Life Science and Research Center for Life Science, College of Science, Rikkyo University, Toshima-ku, Nishi-ikebukuro 3-34-1, Tokyo 171-8501, Japan.
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Sekine
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics / Q000254:growth & development* / Q000528:radiation effects; D015870:Gene Expression; D050505:Mutant Proteins / Q000235:genetics / Q000378:metabolism; D020125:Mutation, Missense; D012269:Ribosomal Proteins / Q000172:deficiency* / Q000235:genetics; D013171:Spores, Bacterial / Q000235:genetics / Q000254:growth & development* / Q000528:radiation effects; D013489:Suppression, Genetic; D013696:Temperature
chemical_list: D050505:Mutant Proteins; D012269:Ribosomal Proteins; C029466:ribosomal protein L2