title: Development and validation of a 4-plex antibody assay for simultaneous detection of IgG antibodies against Torque teno sus virus 1 (TTSuV1), TTSuV2, and porcine reproductive and respiratory syndrome virus types 1 and 2.
abstract: A fluorescent microbead-based immunoassay (FMIA) for simultaneous detection of IgG antibodies against Torque teno sus virus 1 (TTSuV1), TTSuV2, porcine reproductive and respiratory syndrome virus type 1 (PRRSV-1) and PRRSV-2 was developed. Serum samples were obtained over time from 20 pigs. Twelve of 20 were exposed to TTSuV2 on day 0, 20/20 were vaccinated with a PRRSV-2 vaccine on day 35, and 20/20 were exposed to PRRSV-2 on day 63. Anti-TTSuV antibodies were detected in 30% of the pigs on day 0, and 90% by day 35. All PRRSV-2 vaccinated pigs had detectable anti-PRRSV-2 IgG 21 days after vaccination. Field samples from 17 farms were also tested. The seroprevalence of both PRRSV and TTSuV increased with age. Comparison of the PRRSV-2 FMIA to an ELISA revealed good correlation in young pigs but a high rate of false positives in older pigs. Cross-reaction between PRRSV types was a problem.
authors.affiliation: Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, 1600 S. 16th Street, Ames, IA 50011, USA.
authors.forename: Luis G
authors.initials: LG
authors.lastname: Giménez-Lirola
authors.affiliation: Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, 1600 S. 16th Street, Ames, IA 50011, USA; Laboratório de Pesquisa em Virologia Animal, Departamento de Medicina Veterinária Preventiva, Universidade Federal de Minas Gerais, Belo Horizonte, MG 31270-901, Brazil.
authors.forename: Priscilla F
authors.initials: PF
authors.lastname: Gerber
authors.affiliation: Department of Diagnostic Medicine and Pathobiology, Kansas State University, Manhattan, KS 66506, USA.
authors.forename: Raymond R
authors.initials: RR
authors.lastname: Rowland
authors.affiliation: Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, 1600 S. 16th Street, Ames, IA 50011, USA.
authors.forename: Patrick G
authors.initials: PG
authors.lastname: Halbur
authors.affiliation: Department of Biomedical Sciences and Pathobiology, Center for Molecular Medicine and Infectious Diseases, College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA; Institute of Preventive Veterinary Medicine & Key Laboratory of Animal Virology of Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Yao-Wei
authors.initials: YW
authors.lastname: Huang
authors.affiliation: Department of Biomedical Sciences and Pathobiology, Center for Molecular Medicine and Infectious Diseases, College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA.
authors.forename: Xiang-Jin
authors.initials: XJ
authors.lastname: Meng
authors.affiliation: Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, 1600 S. 16th Street, Ames, IA 50011, USA; The Roslin Institute and The Royal (Dick) School of Veterinary Studies, University of Edinburgh, Midlothian EH25 9RG, UK. Electronic address: tanjaopr@iastate.edu.
authors.forename: Tanja
authors.initials: T
authors.lastname: Opriessnig
mesh_terms: D000818:Animals; D000956:Antigens, Viral; D036022:Capsid Proteins; D004266:DNA Virus Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D007118:Immunoassay / Q000379:methods / Q000592:standards / Q000662:veterinary*; D007074:Immunoglobulin G / Q000097:blood*; D008863:Microspheres; D019590:Nucleocapsid Proteins; D019318:Porcine Reproductive and Respiratory Syndrome / Q000175:diagnosis* / Q000821:virology; D019316:Porcine respiratory and reproductive syndrome virus / Q000302:isolation & purification*; D011897:Random Allocation; D015203:Reproducibility of Results; D018709:Statistics, Nonparametric; D013552:Swine; D022783:Torque teno virus / Q000302:isolation & purification*
chemical_list: D000956:Antigens, Viral; D036022:Capsid Proteins; D007074:Immunoglobulin G; D019590:Nucleocapsid Proteins
keywords: 4-plex; Antibody detection; Fluorescent microbead-based immunoassay; PRRSV; Porcine reproductive and respiratory syndrome virus; TTSuV; Torque teno sus virus