title: Molecular and functional consequences of mutations in the central helix of cardiac troponin C.
abstract: The objective of this work was to investigate the role of acidic residues within the exposed middle segment of the central helix of cTnC in (1) cTnC-cTnI interactions, (2) Ca(2+) binding and exchange with the regulatory N-domain of cTnC in increasingly complex biochemical systems, and (3) ability of the cTn complex to regulate actomyosin ATPase. In order to achieve this objective, we introduced the D87A/D88A and E94A/E95A/E96A mutations into the central helix of cTnC. The D87A/D88A and E94A/E95A/E96A mutations decreased affinity of cTnC for the regulatory region of cTnI. The Ca(2+) sensitivity of the regulatory N-domain of isolated cTnC was decreased by the D87A/D88A, but not E94A/E95A/E96A mutation. However, both the D87A/D88A and E94A/E95A/E96A mutations desensitized the cTn complex and reconstituted thin filaments to Ca(2+). Decreases in the Ca(2+) sensitivity of the cTn complex and reconstituted thin filaments were, at least in part, due to faster rates of Ca(2+) dissociation. In addition, the D87A/D88A and E94A/E95A/E96A mutations desensitized actomyosin ATPase to Ca(2+), and decreased maximal actomyosin ATPase activity. Thus, our results indicate that conserved acidic residues within the exposed middle segment of the central helix of cTnC are important for the proper regulatory function of the cTn complex.
authors.affiliation: Department of Pharmacological and Pharmaceutical Sciences, University of Houston, Houston, TX 77004, United States.
authors.forename: Nicholas
authors.initials: N
authors.lastname: Swindle
authors.affiliation: Department of Biology, Wingate University, Wingate, NC 28174, United States.
authors.forename: Acchia N J
authors.initials: AN
authors.lastname: Albury
authors.affiliation: Department of Pharmacological and Pharmaceutical Sciences, University of Houston, Houston, TX 77004, United States.
authors.forename: Belal
authors.initials: B
authors.lastname: Baroud
authors.affiliation: Department of Pharmacological and Pharmaceutical Sciences, University of Houston, Houston, TX 77004, United States.
authors.forename: Maryam
authors.initials: M
authors.lastname: Burney
authors.affiliation: Department of Pharmacological and Pharmaceutical Sciences, University of Houston, Houston, TX 77004, United States. Electronic address: sbtikunova@uh.edu.
authors.forename: Svetlana B
authors.initials: SB
authors.lastname: Tikunova
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D006801:Humans; D008958:Models, Molecular; D009206:Myocardium / Q000378:metabolism*; D009218:Myosins / Q000378:metabolism; D017354:Point Mutation; D017433:Protein Structure, Secondary; D019209:Troponin C / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D019210:Troponin I / Q000378:metabolism*
chemical_list: D019209:Troponin C; D019210:Troponin I; D009218:Myosins; D002118:Calcium
keywords: Calcium binding; Central helix; Fluorescence; Troponin C; Troponin I