title: Effect of EGb761 on light-damaged retinal pigment epithelial cells.
abstract: OBJECTIVE
  To investigate the protective mechanism of Gingko Biloba extract (EGb761) on the ability of retinal pigment epithelial (RPE) cells to resist light-induced damage in a comparative proteomics study.
  
  
  METHODS
  Human RPE cells (ARPE-19) were randomly distributed to one of three groups: normal control (NC group) and light-damaged model without or with EGb761 group (M and ME groups, respectively). The light-damaged model was formed by exposing to white light (2 200±300)lx for 6h. The RPE cells in ME group were conducted with EGb 761 (100µg/mL) before light exposure. The soluble cellular proteins extracting from each groups were separated by two-dimensional electrophoresis and stained by silver staining. Different proteins in the profiles of the gels were analyzed by Image Master Software. Two-fold expressing protein spots were identified by Matrix-assisted laser desorption/ionization tandem time-of-flight (MALDI-TOF/TOF) mass spectrometry.
  
  
  RESULTS
  NC, M and ME groups displayed 1 892±71, 2 145±23 and 2 216±85 protein spots, respectively. We identified 33 proteins with different expression levels between the NC and M groups, 25 proteins between the M and ME groups, and 11 proteins between the NC and ME groups. MALDI-TOF/TOF mass spectrometry successfully identified 16 proteins, including metabolic enzymes, cytoskeletal proteins, anti-oxidation proteins, and others.
  
  
  CONCLUSIONS
  Differences in some important proteins, such as cathepsin B, heat shock protein, and cytochrome c reductase, indicated that multiple pathways may be induced in light-damaged RPE cells and the protective effect of EGb761.
authors.affiliation: Department of Ophthalmology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China.
authors.forename: Yun-Yun
authors.initials: YY
authors.lastname: Zhou
authors.affiliation: Department of Ophthalmology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China.
authors.forename: Chang-Zheng
authors.initials: CZ
authors.lastname: Chen
authors.affiliation: Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, Guangdong Province, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Su
authors.affiliation: Department of Ophthalmology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Ophthalmology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China.
authors.forename: Zuo-Hui-Zi
authors.initials: ZH
authors.lastname: Yi
authors.affiliation: Department of Ophthalmology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China.
authors.forename: Hang
authors.initials: H
authors.lastname: Qi
authors.affiliation: Department of Ophthalmology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Weng
authors.affiliation: Department of Ophthalmology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China.
authors.forename: Yi-Qiao
authors.initials: YQ
authors.lastname: Xing
keywords: EGb761; light damage; mass spectrographic analysis; protein; retinal pigment epithelial cells