title: Preparation of colloidal gold immunochromatographic strip for detection of Paragonimiasis skrjabini.
abstract: BACKGROUND
  Paragonimiasis is a food-borne trematodiasis, a serious public health issue and a neglected tropical disease. Paragonimus skrjabini is a unique species found in China. Unlike paragonimiasis westermani, it is nearly impossible to make a definitive diagnosis for paragonimiasis skrjabini by finding eggs in sputum or feces. Immunodiagnosis is the best choice to detect paragonimiasis skrjabini. There is an urgent need to develop a novel, rapid and simple immunoassay for large-scale screening patients in endemic areas.
  
  
  RESULTS
  To develop a rapid, simple immunodiagnostic assay for paragonimiasis, rabbit anti-human IgG was conjugated to colloidal gold particles and used to detect antibodies in the sera of paragonimiasis patients. The synthesis and identification of colloidal gold particles and antibody-colloidal gold conjugates were performed. The size of colloidal gold particles was examined using a transmission electron microscope (TEM). The average diameter of colloidal gold particles was 17.46 nm with a range of 14.32-21.80 nm according to the TEM images. The formation of antibody-colloidal gold conjugates was monitored by UV/Vis spectroscopy. Excretory-secretory (ES) antigen of Paragonimus skrjabini was coated on nitrocellulose membrane as the capture line. Recombinant Staphylococcus protein A was used to prepare the control line. This rapid gold immunochromatographic strip was assembled in regular sequence through different accessories sticked on PVC board. The relative sensitivity and specificity of the strip was 94.4% (51/54) and 94.1% (32/34) respectively using ELISA as the standard method. Its stability and reproducibility were quite excellent after storage of the strip at 4°C for 6 months.
  
  
  CONCLUSIONS
  Immunochromatographic strip prepared in this study can be used in a rapid one-step immunochromatographic assay, which is instantaneous and convenient.
authors.affiliation: Institute of Tropical Medicine, Third Military Medical University, Chongqing, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Pathogenic Biology, Luzhou Medical College, Sichuan, China.
authors.forename: Lifang
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Physics, Chengdu Medical College, Sichuan, China.
authors.forename: Jianwei
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Pathogenic Biology, Luzhou Medical College, Sichuan, China.
authors.forename: Guangxi
authors.initials: G
authors.lastname: Wang
authors.affiliation: Department of Pathogenic Biology, Luzhou Medical College, Sichuan, China.
authors.forename: Wenbi
authors.initials: W
authors.lastname: Chen
authors.affiliation: Department of Pathogenic Biology, Third Military Medical University, Chongqing, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
authors.affiliation: Institute of Tropical Medicine, Third Military Medical University, Chongqing, China; Department of Pathogenic Biology, Third Military Medical University, Chongqing, China.
authors.forename: Xilin
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000737:chemistry* / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000276:immunology; D002846:Chromatography, Affinity / Q000379:methods* / Q000592:standards; D003102:Colloids; D005260:Female; D006046:Gold / Q000737:chemistry; D006801:Humans; D010237:Paragonimiasis / Q000175:diagnosis* / Q000276:immunology / Q000469:parasitology; D010238:Paragonimus / Q000276:immunology / Q000302:isolation & purification*; D010316:Particle Size; D011817:Rabbits; D011934:Reagent Strips; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000909:Antibodies, Helminth; D000911:Antibodies, Monoclonal; D003102:Colloids; D011934:Reagent Strips; D006046:Gold