title: Integrating metabolomics profiling measurements across multiple biobanks.
abstract: To optimize the quality of large scale mass-spectrometry based metabolomics data obtained from semiquantitative profiling measurements, it is important to use a strategy in which dedicated measurement designs are combined with a strict statistical quality control regime. This assures consistently high-quality results across measurements from individual studies, but semiquantitative data have been so far only comparable for samples measured within the same study. To enable comparability and integration of semiquantitative profiling data from different large scale studies over the time course of years, the measurement and quality control strategy has to be extended. We introduce a strategy to allow the integration of semiquantitative profiling data from different studies. We demonstrate that lipidomics data generated in samples from three different large biobanks acquired in the time course of 3 years can be effectively combined when using an appropriate measurement design and transfer model. This strategy paves the way toward an integrative usage of semiquantitative metabolomics data sets of multiple studies to validate biological findings in another study and/or to increase the statistical power for discovery of biomarkers or pathways by combining studies.
authors.affiliation: Division Analytical Biosciences, Leiden Academic Center for Drug Research, Einsteinweg 55, 2333CC Leiden, The Netherlands.
authors.forename: A D
authors.initials: AD
authors.lastname: Dane
authors.forename: M M W B
authors.initials: MM
authors.lastname: Hendriks
authors.forename: T H
authors.initials: TH
authors.lastname: Reijmers
authors.forename: A C
authors.initials: AC
authors.lastname: Harms
authors.forename: J
authors.initials: J
authors.lastname: Troost
authors.forename: R J
authors.initials: RJ
authors.lastname: Vreeken
authors.forename: D I
authors.initials: DI
authors.lastname: Boomsma
authors.forename: C M
authors.initials: CM
authors.lastname: van Duijn
authors.forename: E P
authors.initials: EP
authors.lastname: Slagboom
authors.forename: T
authors.initials: T
authors.lastname: Hankemeier
mesh_terms: D002853:Chromatography, Liquid; D013058:Mass Spectrometry; D055432:Metabolomics; D011786:Quality Control; D014015:Tissue Banks