title: The role of interleukin-1β in the pentylenetetrazole-induced kindling of seizures, in the rat hippocampus.
abstract: Because the contribution of inflammatory mediators to seizure disorders is unclear, we investigated the changes in the expression of interleukin-1β (IL-β) and its receptor - IL-1 receptor type 1 (IL-1R1), tumour necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in the rat hippocampus at different stages of pentylenetetrazole (PTZ)-induced kindling. The occurrence and progressive development of seizures were induced by repeated systemic administration of PTZ, a non-competitive antagonist of the γ-aminobutyric acid type A (GABAA) receptor at a subconvulsive dose of 30 mg/kg. We also examined the effects of continuous intracerebroventricular administration of IL-1β and lipopolysaccharide (LPS) in this model of epilepsy using subcutaneously implanted osmotic mini-pumps. We observed enhanced IL-1R1 expression in the dentate gyrus (DG) at different stages of kindling, whereas the elevated IL-1β level was distinctive to fully kindled seizures. We did not detect significant changes in the concentration of IL-6 or TNF-α throughout the kindling process. LPS accelerated transiently the process of kindling, while IL-1β showed a predisposition to delay kindling acquisition. Our study supports the concept of seizure-related modifications in brain cytokine production during epileptogenesis. Although some evidence indicates a proconvulsant property of IL-1β activity, it cannot be ruled out that the alterations in IL-1 system reflect the activation of endogenous protective mechanisms with respect to the kindling of seizures.
authors.affiliation: Department of Experimental and Clinical Pharmacology, Medical University of Warsaw, 26/28 Krakowskie Przedmieście Street, 00-927 Warsaw, Poland. Electronic address: kkolosow@tlen.pl.
authors.forename: Karolina
authors.initials: K
authors.lastname: Kołosowska
authors.affiliation: Department of Experimental and Clinical Pharmacology, Medical University of Warsaw, 26/28 Krakowskie Przedmieście Street, 00-927 Warsaw, Poland; Department of Neurochemistry, Institute of Psychiatry and Neurology, 9 Sobieskiego Street, 02-957 Warsaw, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Maciejak
authors.affiliation: Department of Experimental and Clinical Pharmacology, Medical University of Warsaw, 26/28 Krakowskie Przedmieście Street, 00-927 Warsaw, Poland.
authors.forename: Janusz
authors.initials: J
authors.lastname: Szyndler
authors.affiliation: Department of Neurochemistry, Institute of Psychiatry and Neurology, 9 Sobieskiego Street, 02-957 Warsaw, Poland.
authors.forename: Danuta
authors.initials: D
authors.lastname: Turzyńska
authors.affiliation: Department of Neurochemistry, Institute of Psychiatry and Neurology, 9 Sobieskiego Street, 02-957 Warsaw, Poland.
authors.forename: Alicja
authors.initials: A
authors.lastname: Sobolewska
authors.affiliation: Department of Experimental and Clinical Pharmacology, Medical University of Warsaw, 26/28 Krakowskie Przedmieście Street, 00-927 Warsaw, Poland; Department of Neurochemistry, Institute of Psychiatry and Neurology, 9 Sobieskiego Street, 02-957 Warsaw, Poland.
authors.forename: Adam
authors.initials: A
authors.lastname: Płaźnik
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism / Q000503:physiopathology*; D053583:Interleukin-1beta / Q000378:metabolism* / Q000494:pharmacology; D015850:Interleukin-6 / Q000378:metabolism; D007696:Kindling, Neurologic / Q000187:drug effects*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D010433:Pentylenetetrazole / Q000009:adverse effects*; D051381:Rats; D053573:Receptors, Interleukin-1 Type I / Q000378:metabolism; D012640:Seizures / Q000139:chemically induced* / Q000378:metabolism* / Q000503:physiopathology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D053583:Interleukin-1beta; D015850:Interleukin-6; D008070:Lipopolysaccharides; D053573:Receptors, Interleukin-1 Type I; D014409:Tumor Necrosis Factor-alpha; D010433:Pentylenetetrazole
keywords: Cytokines; Hippocampus; IL-1β; Kindling of seizures; PTZ; Rats