title: MALAT1 promotes the proliferation and metastasis of gallbladder cancer cells by activating the ERK/MAPK pathway.
abstract: Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1), a long non-coding RNA (lncRNA), is associated with metastasis and is an independent prognostic factor for lung cancer. Recent studies have demonstrated that MALAT1 plays an important role in other malignancies. However, little is known about the role of MALAT1 in gallbladder carcinoma (GBC), which is the most common cancer of the biliary tract and has an extremely poor prognosis. In this study, we focused on the expression, biological functions and mechanism of MALAT1 in GBC and found that MALAT1 was significantly upregulated in GBC tissues compared with corresponding non-cancerous tissues. Knockdown of MALAT1 in GBC cell lines using lentivirus-mediated RNA interference significantly inhibited the proliferation and metastasis of the GBC cells both in vitro and in vivo. Furthermore, ERK/MAPK pathway was found to be inactivated in the GBC cell lines after MALAT1 knockdown. These results indicated that MALAT1 might serve as an oncogenic lncRNA that promotes proliferation and metastasis of GBC and activates the ERK/MAPK pathway.
authors.affiliation: Department of General Surgery; Xinhua Hospital; Shanghai Jiao Tong University School of Medicine; Shanghai, PR China; Laboratory of General Surgery, Xinhua Hospital; Shanghai Jiao Tong University School of Medicine; Shanghai, PR China; Institute of Biliary Tract Disease; Shanghai Jiao Tong University School of Medicine; Shanghai, PR China.
authors.forename: Xiang-Song
authors.initials: XS
authors.lastname: Wu
authors.affiliation: Department of General Surgery; Xinhua Hospital; Shanghai Jiao Tong University School of Medicine; Shanghai, PR China; Laboratory of General Surgery, Xinhua Hospital; Shanghai Jiao Tong University School of Medicine; Shanghai, PR China; Institute of Biliary Tract Disease; Shanghai Jiao Tong University School of Medicine; Shanghai, PR China.
authors.forename: Xu-An
authors.initials: XA
authors.lastname: Wang
authors.affiliation: Department of General Surgery; Xinhua Hospital; Shanghai Jiao Tong University School of Medicine; Shanghai, PR China; Laboratory of General Surgery, Xinhua Hospital; Shanghai Jiao Tong University School of Medicine; Shanghai, PR China; Institute of Biliary Tract Disease; Shanghai Jiao Tong University School of Medicine; Shanghai, PR China.
authors.forename: Wen-Guang
authors.initials: WG
authors.lastname: Wu
authors.affiliation: Department of General Surgery; Xinhua Hospital; Shanghai Jiao Tong University School of Medicine; Shanghai, PR China; Laboratory of General Surgery, Xinhua Hospital; Shanghai Jiao Tong University School of Medicine; Shanghai, PR China; Institute of Biliary Tract Disease; Shanghai Jiao Tong University School of Medicine; Shanghai, PR China.
authors.forename: Yun-Ping
authors.initials: YP
authors.lastname: Hu
authors.affiliation: Department of General Surgery; Xinhua Hospital; Shanghai Jiao Tong University School of Medicine; Shanghai, PR China; Laboratory of General Surgery, Xinhua Hospital; Shanghai Jiao Tong University School of Medicine; Shanghai, PR China; Institute of Biliary Tract Disease; Shanghai Jiao Tong University School of Medicine; Shanghai, PR China.
authors.forename: Mao-Lan
authors.initials: ML
authors.lastname: Li
authors.affiliation: Department of General Surgery; Xinhua Hospital; Shanghai Jiao Tong University School of Medicine; Shanghai, PR China; Laboratory of General Surgery, Xinhua Hospital; Shanghai Jiao Tong University School of Medicine; Shanghai, PR China; Institute of Biliary Tract Disease; Shanghai Jiao Tong University School of Medicine; Shanghai, PR China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Ding
authors.affiliation: Department of General Surgery; Xinhua Hospital; Shanghai Jiao Tong University School of Medicine; Shanghai, PR China; Laboratory of General Surgery, Xinhua Hospital; Shanghai Jiao Tong University School of Medicine; Shanghai, PR China; Institute of Biliary Tract Disease; Shanghai Jiao Tong University School of Medicine; Shanghai, PR China.
authors.forename: Hao
authors.initials: H
authors.lastname: Weng
authors.affiliation: Department of General Surgery; Xinhua Hospital; Shanghai Jiao Tong University School of Medicine; Shanghai, PR China; Laboratory of General Surgery, Xinhua Hospital; Shanghai Jiao Tong University School of Medicine; Shanghai, PR China; Institute of Biliary Tract Disease; Shanghai Jiao Tong University School of Medicine; Shanghai, PR China.
authors.forename: Yi-Jun
authors.initials: YJ
authors.lastname: Shu
authors.affiliation: Department of General Surgery; Xinhua Hospital; Shanghai Jiao Tong University School of Medicine; Shanghai, PR China; Laboratory of General Surgery, Xinhua Hospital; Shanghai Jiao Tong University School of Medicine; Shanghai, PR China; Institute of Biliary Tract Disease; Shanghai Jiao Tong University School of Medicine; Shanghai, PR China.
authors.forename: Tian-Yu
authors.initials: TY
authors.lastname: Liu
authors.affiliation: Department of General Surgery; Xinhua Hospital; Shanghai Jiao Tong University School of Medicine; Shanghai, PR China; Laboratory of General Surgery, Xinhua Hospital; Shanghai Jiao Tong University School of Medicine; Shanghai, PR China; Institute of Biliary Tract Disease; Shanghai Jiao Tong University School of Medicine; Shanghai, PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Jiang
authors.affiliation: Department of General Surgery; Xinhua Hospital; Shanghai Jiao Tong University School of Medicine; Shanghai, PR China; Laboratory of General Surgery, Xinhua Hospital; Shanghai Jiao Tong University School of Medicine; Shanghai, PR China; Institute of Biliary Tract Disease; Shanghai Jiao Tong University School of Medicine; Shanghai, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Department of General Surgery; Xinhua Hospital; Shanghai Jiao Tong University School of Medicine; Shanghai, PR China; Laboratory of General Surgery, Xinhua Hospital; Shanghai Jiao Tong University School of Medicine; Shanghai, PR China; Institute of Biliary Tract Disease; Shanghai Jiao Tong University School of Medicine; Shanghai, PR China.
authors.forename: Run-Fa
authors.initials: RF
authors.lastname: Bao
authors.affiliation: Department of General Surgery; Xinhua Hospital; Shanghai Jiao Tong University School of Medicine; Shanghai, PR China; Laboratory of General Surgery, Xinhua Hospital; Shanghai Jiao Tong University School of Medicine; Shanghai, PR China; Institute of Biliary Tract Disease; Shanghai Jiao Tong University School of Medicine; Shanghai, PR China.
authors.forename: Jia-Sheng
authors.initials: JS
authors.lastname: Mu
authors.affiliation: Department of General Surgery; Xinhua Hospital; Shanghai Jiao Tong University School of Medicine; Shanghai, PR China; Laboratory of General Surgery, Xinhua Hospital; Shanghai Jiao Tong University School of Medicine; Shanghai, PR China; Institute of Biliary Tract Disease; Shanghai Jiao Tong University School of Medicine; Shanghai, PR China.
authors.forename: Zhu-Jun
authors.initials: ZJ
authors.lastname: Tan
authors.affiliation: Department of Gastrointestinal Surgery; Shaoxing People's Hospital; Shaoxing, PR China.
authors.forename: Feng
authors.initials: F
authors.lastname: Tao
authors.affiliation: Department of General Surgery; Xinhua Hospital; Shanghai Jiao Tong University School of Medicine; Shanghai, PR China; Laboratory of General Surgery, Xinhua Hospital; Shanghai Jiao Tong University School of Medicine; Shanghai, PR China; Institute of Biliary Tract Disease; Shanghai Jiao Tong University School of Medicine; Shanghai, PR China.
authors.forename: Ying-Bin
authors.initials: YB
authors.lastname: Liu
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism / Q000556:secondary*; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D005260:Female; D005706:Gallbladder Neoplasms / Q000378:metabolism / Q000473:pathology*; D055785:Gene Knockdown Techniques; D006801:Humans; D020935:MAP Kinase Signaling System; D008297:Male; D008875:Middle Aged; D062085:RNA, Long Noncoding / Q000502:physiology*
chemical_list: C557041:MALAT1 long non-coding RNA, human; D062085:RNA, Long Noncoding
keywords: ERK; MALAT1; MAPK; gallbladder carcinoma; lncRNA; metastasis; proliferation