title: A spatial patternable macroporous hydrogel with cell-affinity domains to enhance cell spreading and differentiation.
abstract: Cell adhesion and spreading are two essential factors for anchorage-dependent cells such as osteocytes. An adhesive macroporous hydrogel system, in which cell-affinitive domains and sufficient cytoskeleton reorganization space were simultaneously constructed, was proposed in this report to support cell adhesion and spreading, respectively, and facilitate cell differentiation and function establishment eventually. The adhesive macroporous alginate hydrogel was developed by RGD peptide graft and gelatin microspheres hybridization to generate cellular adhesion sites and highly interconnected macropores. The successful stretched morphology and enhanced osteogenic differentiation of MG-63 cells in this modified alginate hydrogel showed clearly the feasibility that cell function may be effectively facilitated. Besides, this hydrogel model can be further applied to construct complex micropatterned structure, such as individual microgels in shapes of circle, square, cross and ring, and osteon-like structure containing both osteogenic and vascularized area generated by a double-ring assembly. These results should provide this adhesive macroporous photocrosslinkable hydrogel system as potential three-dimensional scaffolds for guiding tissue formation, especially for the bioengineering of tissues that have multiple cell types and require precisely defined cell-cell and cell-substrate interactions.
authors.affiliation: National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, Sichuan, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Sun
authors.affiliation: National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, Sichuan, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Wei
authors.affiliation: National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, Sichuan, China.
authors.forename: Yuda
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, Sichuan, China.
authors.forename: Meiling
authors.initials: M
authors.lastname: Zhong
authors.affiliation: National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, Sichuan, China.
authors.forename: Yicong
authors.initials: Y
authors.lastname: Zuo
authors.affiliation: National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, Sichuan, China. Electronic address: hsfan@scu.edu.cn.
authors.forename: Hongsong
authors.initials: H
authors.lastname: Fan
authors.affiliation: National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, Sichuan, China.
authors.forename: Xingdong
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000464:Alginates / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion; D002454:Cell Differentiation; D002460:Cell Line; D002465:Cell Movement; D005780:Gelatin / Q000737:chemistry*; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D009842:Oligopeptides / Q000737:chemistry*; D010006:Osteoblasts / Q000166:cytology*; D010012:Osteogenesis; D016062:Porosity
chemical_list: D000464:Alginates; D001672:Biocompatible Materials; D006603:Hexuronic Acids; D009842:Oligopeptides; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; C047981:arginyl-glycyl-aspartic acid; D020723:Glucuronic Acid; D005780:Gelatin
keywords: Cell adhesion; Cell spreading; Hydrogel; Osteon-like; Photocrosslinking; RGD