title: Verification of the anatomy and newly discovered histology of the G-spot complex.
abstract: OBJECTIVE
  To expand the anatomical investigations of the G-spot and to assess the G-spot's characteristic histological and immunohistochemical features.
  
  
  METHODS
  An observational study.
  
  
  METHODS
  International multicentre.
  
  
  METHODS
  Eight consecutive fresh human female cadavers.
  
  
  METHODS
  Anterior vaginal wall dissections were executed and G-spot microdissections were performed. All specimens were stained with haematoxylin and eosin (H&E). The tissues of two women were selected at random for immunohistochemical staining.
  
  
  METHODS
  The primary outcome measure was to document the anatomy of the G-spot. The secondary outcome measures were to identify the histology of the G-spot and to determine whether histological samples stained with H&E are sufficient to identify the G-spot.
  
  
  RESULTS
  The anatomical existence of the G-spot was identified in all women and was in a diagonal plane. In seven (87.5%) and one (12.5%) of the women the G-spot complex was found on the left or right side, respectively. The G-spot was intimately fused with vessels, creating a complex. A large tangled vein-like vascular structure resembled an arteriovenous malformation and there were a few smaller feeding arteries. A band-like structure protruded from the tail of the G-spot. The size of the G-spot varied. Histologically, the G-spot was determined as a neurovascular complex structure. The neural component contained abundant peripheral nerve bundles and a nerve ganglion. The vascular component comprised large vein-like vessels and smaller feeding arteries. Circular and longitudinal muscles covered the G-complex.
  
  
  CONCLUSIONS
  The anatomy of the G-spot complex was confirmed. The histology of the G-spot presents as neurovascular tissues with a nerve ganglion. H&E staining is sufficient for the identification of the G-spot complex.
authors.affiliation: Institute of Gynecology Inc., St Petersburg, FL, USA.
authors.forename: A
authors.initials: A
authors.lastname: Ostrzenski
authors.forename: P
authors.initials: P
authors.lastname: Krajewski
authors.forename: P
authors.initials: P
authors.lastname: Ganjei-Azar
authors.forename: A J
authors.initials: AJ
authors.lastname: Wasiutynski
authors.forename: M N
authors.initials: MN
authors.lastname: Scheinberg
authors.forename: S
authors.initials: S
authors.lastname: Tarka
authors.forename: M
authors.initials: M
authors.lastname: Fudalej
mesh_terms: D000328:Adult; D002102:Cadaver; D002987:Clitoris / Q000033:anatomy & histology* / Q000098:blood supply / Q000294:innervation; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D009412:Nerve Fibers / Q000502:physiology*; D009948:Orgasm / Q000502:physiology*; D017933:Peripheral Nervous System / Q000502:physiology; D014621:Vagina / Q000033:anatomy & histology* / Q000098:blood supply / Q000294:innervation
keywords: G-spot; G-spot anatomy; G-spot histology; G-spot immunohistochemistry; Gräfenberg's zone; female erectile body; female prostate; female sexual function; vaginal anatomy