title: Viral replication and lung lesions in BALB/c mice experimentally inoculated with avian metapneumovirus subgroup C isolated from chickens.
abstract: Avian metapneumovirus (aMPV) emerged as an important respiratory pathogen causing acute respiratory tract infection in avian species. Here we used a chicken aMPV subgroup C (aMPV/C) isolate to inoculate experimentally BALB/c mice and found that the aMPV/C can efficiently replicate and persist in the lungs of mice for at least 21 days with a peak viral load at day 6 postinoculation. Lung pathological changes were characterized by increased inflammatory cells. Immunochemical assay showed the presence of viral antigens in the lungs and significant upregulation of pulmonary inflammatory cytokines and chemokines including MCP-1, MIP-1α, RANTES, IL-1β, IFN-γ, and TNF-α were detected following inoculation. These results indicate for the first time that chicken aMPV/C may replicate in the lung of mice. Whether aMPV/C has potential as zoonotic pathogen, further investigation will be required.
authors.affiliation: Beijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Wei
authors.affiliation: Beijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing, People's Republic of China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Zhu
authors.affiliation: College of Veterinary Medicine, China Agricultural University, Beijing, People's Republic of China.
authors.forename: Ruiping
authors.initials: R
authors.lastname: She
authors.affiliation: College of Veterinary Medicine, China Agricultural University, Beijing, People's Republic of China.
authors.forename: Fengjiao
authors.initials: F
authors.lastname: Hu
authors.affiliation: Beijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Beijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing, People's Republic of China.
authors.forename: Xu
authors.initials: X
authors.lastname: Yan
authors.affiliation: Beijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing, People's Republic of China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Beijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing, People's Republic of China.
authors.forename: Shuhang
authors.initials: S
authors.lastname: Liu
authors.affiliation: Beijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing, People's Republic of China.
authors.forename: Rong
authors.initials: R
authors.lastname: Quan
authors.affiliation: Beijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing, People's Republic of China.
authors.forename: Zixuan
authors.initials: Z
authors.lastname: Li
authors.affiliation: College of Veterinary Medicine, China Agricultural University, Beijing, People's Republic of China.
authors.forename: Fang
authors.initials: F
authors.lastname: Du
authors.affiliation: Molecular Virology Laboratory, QIMR Berghofer Medical Research Institute, Queensland, Australia.
authors.forename: Ting
authors.initials: T
authors.lastname: Wei
authors.affiliation: Beijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing, People's Republic of China.
authors.forename: Jue
authors.initials: J
authors.lastname: Liu
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology; D018925:Chemokines / Q000378:metabolism; D002645:Chickens / Q000821:virology*; D005260:Female; D007150:Immunohistochemistry; D007700:Kinetics; D008168:Lung / Q000473:pathology* / Q000821:virology*; D029121:Metapneumovirus / Q000276:immunology / Q000302:isolation & purification* / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D018184:Paramyxoviridae Infections / Q000276:immunology / Q000473:pathology / Q000821:virology*; D013696:Temperature; D014779:Virus Replication / Q000502:physiology*
chemical_list: D057134:Antibodies, Neutralizing; D000956:Antigens, Viral; D018925:Chemokines