title: Thin-layer polymer wrapped enzymes encapsulated in hierarchically mesoporous silica with high activity and enhanced stability.
abstract: A novel soft-hard cooperative approach was developed to synthesize bioactive mesoporous composite by pre-wrapping Penicillin G amidase with poly(acrylaimde) nanogel skin and subsequently incorporating such Penicillin G amidase nanocapsules into hierarchically mesoporous silica. The as-received bioactive mesoporous composite exhibited comparable activity and extraordinarily high stability in comparison with native Penicillin G amidase and could be used repetitively in the water-medium hydrolysis of penicillin G potassium salt. Furthermore, this strategy could be extended to the synthesis of multifunctional bioactive mesoporous composite by simultaneously introducing glucose oxidase nanocapsules and horseradish peroxidase nanocapsules into hierarchically mesoporous silica, which demonstrated a synergic effect in one-pot tandem oxidation reaction. Improvements in the catalytic performances were attributed to the combinational unique structure from soft polymer skin and hard inorganic mesoporous silica shell, which cooperatively helped enzyme molecules to retain their appropriate geometry and simultaneously decreased the enzyme-support negative interaction and mass transfer limitation under heterogeneous conditions.
authors.affiliation: The Education Ministry Key Lab of Resource Chemistry and Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Normal University, Shanghai 200234, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Zhang
authors.affiliation: The Education Ministry Key Lab of Resource Chemistry and Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Normal University, Shanghai 200234, China.
authors.forename: Meitao
authors.initials: M
authors.lastname: Wang
authors.affiliation: The Education Ministry Key Lab of Resource Chemistry and Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Normal University, Shanghai 200234, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Liang
authors.affiliation: The Education Ministry Key Lab of Resource Chemistry and Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Normal University, Shanghai 200234, China.
authors.forename: Huangyong
authors.initials: H
authors.lastname: Jiang
authors.affiliation: The Education Ministry Key Lab of Resource Chemistry and Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Normal University, Shanghai 200234, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Shen
authors.affiliation: The Education Ministry Key Lab of Resource Chemistry and Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Normal University, Shanghai 200234, China.
authors.forename: Hexing
authors.initials: H
authors.lastname: Li
mesh_terms: D000180:Acrylic Resins / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D055162:Biocatalysis; D002470:Cell Survival / Q000187:drug effects; D004339:Drug Compounding; D004795:Enzyme Stability; D005949:Glucose Oxidase / Q000737:chemistry*; D006367:HeLa Cells; D006735:Horseradish Peroxidase / Q000737:chemistry*; D006801:Humans; D007700:Kinetics; D053769:Nanocapsules / Q000737:chemistry / Q000648:ultrastructure; D010399:Penicillin Amidase / Q000737:chemistry*; D010400:Penicillin G / Q000737:chemistry; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*; D014867:Water; D000099166:Polyacrylamides
chemical_list: D000180:Acrylic Resins; D005949:Glucose Oxidase; D006735:Horseradish Peroxidase; D053769:Nanocapsules; D010399:Penicillin Amidase; D010400:Penicillin G; D012822:Silicon Dioxide; D014867:Water; D000099166:Polyacrylamides