title: ADAMTS-7 expression increases in the early stage of angiotensin II-induced renal injury in elderly mice.
abstract: OBJECTIVE
  We investigated the recently described family of proteinases, a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTs), and matrix metalloproteinases (MMPs) as inflammatory mediators in inflammatory kidney damage by studying ADAMTS-1, -4, and -7 and MMP-9 expression in elderly mouse kidneys after angiotensin II (Ang II) administration.
  
  
  METHODS
  Ang II (2.5 µg/kg/min) or norepinephrine (8.3 µg/kg/min) was subcutaneously infused in old mice. Renal injury was assessed by hematoxylin-eosin staining, 24-h albuminuria, and immunohistochemistry to evaluate inflammatory cell markers. The mRNA and protein expression of ADAMTS-1, -4, and -7 and MMP-9 were determined using real-time PCR, Western blot, and immunohistochemistry 3 days after Ang II or norepinephrine administration.
  
  
  RESULTS
  Elderly mice in the Ang II group developed hypertension and pathological kidney damage. The mRNA and protein levels of ADAMTS-7 in the Ang II group were 3.3 ± 1.1 (P = 0.019) and 1.6 ± 0.1 (P = 0.047) vs. 1.0 ± 0.1 and 1.0 ± 0.1 in the control group on day 3. In contrast, treatment with the hypertensive agent norepinephrine did not lead to obvious renal damage or an increase in renal ADAMTS-7 expression.
  
  
  CONCLUSIONS
  Renal ADAMTS-7 expression was induced by Ang II in elderly mice. The overexpression of ADATMTS-7 might contribute to early inflammatory kidney damage associated with aging.
authors.affiliation: Department of Cardiology, China-Japan Friendship Hospital, Beijing, P. R. China.
authors.forename: Yan-Xiang
authors.initials: YX
authors.lastname: Gao
authors.forename: Chang-An
authors.initials: CA
authors.lastname: Yu
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Lu
authors.forename: Hong-Mei
authors.initials: HM
authors.lastname: Gao
authors.forename: Geng
authors.initials: G
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Kong
authors.forename: Jingang
authors.initials: J
authors.lastname: Zheng
mesh_terms: D051722:ADAM Proteins / Q000096:biosynthesis* / Q000235:genetics; D000071118:ADAMTS7 Protein; D000375:Aging / Q000502:physiology*; D000804:Angiotensin II; D000818:Animals; D001794:Blood Pressure / Q000502:physiology; D057809:HEK293 Cells; D006801:Humans; D007249:Inflammation / Q000139:chemically induced / Q000378:metabolism; D007674:Kidney Diseases / Q000139:chemically induced* / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009638:Norepinephrine / Q000494:pharmacology; D014662:Vasoconstrictor Agents
chemical_list: D014662:Vasoconstrictor Agents; D000804:Angiotensin II; D051722:ADAM Proteins; D000071118:ADAMTS7 Protein; C496264:Adamts7 protein, mouse; D009638:Norepinephrine