title: Expression pattern of protease activated receptors in lymphoid cells.
abstract: Protease-activated receptors (PARs) are a subfamily of four G-protein-coupled receptors mediating multiple functions. PARs expression was studied in subpopulations of human lymphocytes. Our results indicate that natural killer cells expressed mRNA for PAR₁, PAR₂ and PAR₃, CD4+ T cells expressed PAR₁ and PAR₂, while γδ and CD8+ T cells only expressed PAR₁. PAR₄ was absent at mRNA level and B cells did not express any PAR. Analyses of the cell surface PARs expression by flow cytometry were consistent with the mRNA data and also between different donors. PAR₁ is the most abundant member of the PAR family present in lymphocytes.
authors.affiliation: Instituto Venezolano de Investigaciones Científicas, Caracas 1020A, Venezuela; Institute of Biochemistry, Medical Faculty, Justus-Liebig-University Giessen, D-35392 Giessen, Germany. Electronic address: melopez73@hotmail.com.
authors.forename: Mercedes L
authors.initials: ML
authors.lastname: López
authors.affiliation: Institute for Clinical Immunology and Transfusion Medicine, Justus-Liebig University Giessen, D-35390 Giessen, Germany. Electronic address: natssbu@yahoo.com.
authors.forename: Natalia
authors.initials: N
authors.lastname: Soriano-Sarabia
authors.affiliation: Instituto Venezolano de Investigaciones Científicas, Caracas 1020A, Venezuela. Electronic address: gbruges@gmail.com.
authors.forename: Gustavo
authors.initials: G
authors.lastname: Bruges
authors.affiliation: Instituto Venezolano de Investigaciones Científicas, Caracas 1020A, Venezuela. Electronic address: memct@hotmail.com.
authors.forename: María Elena
authors.initials: ME
authors.lastname: Marquez
authors.affiliation: Institute of Biochemistry, Medical Faculty, Justus-Liebig-University Giessen, D-35392 Giessen, Germany. Electronic address: Klaus.T.Preissner@biochemie.med.uni-giessen.de.
authors.forename: Klaus T
authors.initials: KT
authors.lastname: Preissner
authors.affiliation: Institute of Biochemistry, Medical Faculty, Justus-Liebig-University Giessen, D-35392 Giessen, Germany. Electronic address: Lienhard.Schmitz@biochemie.med.uni-giessen.de.
authors.forename: M Lienhard
authors.initials: ML
authors.lastname: Schmitz
authors.affiliation: Institute for Clinical Immunology and Transfusion Medicine, Justus-Liebig University Giessen, D-35390 Giessen, Germany. Electronic address: holger.hackstein@immunologie.med.uni-giessen.de.
authors.forename: Holger
authors.initials: H
authors.lastname: Hackstein
mesh_terms: D001402:B-Lymphocytes / Q000166:cytology / Q000378:metabolism*; D015704:CD4 Antigens / Q000235:genetics / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000378:metabolism*; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000378:metabolism*; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D007694:Killer Cells, Natural / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000276:immunology; D044463:Receptor, PAR-1 / Q000235:genetics* / Q000276:immunology; D044464:Receptor, PAR-2 / Q000235:genetics* / Q000276:immunology; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000235:genetics / Q000276:immunology; D018179:Receptors, Thrombin / Q000235:genetics* / Q000276:immunology
chemical_list: D015704:CD4 Antigens; D012333:RNA, Messenger; D044463:Receptor, PAR-1; D044464:Receptor, PAR-2; D016692:Receptors, Antigen, T-Cell, gamma-delta; D018179:Receptors, Thrombin; C112908:protease-activated receptor 4
keywords: B cells; CD8+ T cells; FACS; Lymphocytes; Natural killer cells, γδ cells, CD4+ T cells; Protease-activated receptors