title: UCP4C mediates uncoupled respiration in larvae of Drosophila melanogaster.
abstract: Larvae of Drosophila melanogaster reared at 23°C and switched to 14°C for 1 h are 0.5°C warmer than the surrounding medium. In keeping with dissipation of energy, respiration of Drosophila melanogaster larvae cannot be decreased by the F-ATPase inhibitor oligomycin or stimulated by protonophore. Silencing of Ucp4C conferred sensitivity of respiration to oligomycin and uncoupler, and prevented larva-to-adult progression at 15°C but not 23°C. Uncoupled respiration of larval mitochondria required palmitate, was dependent on Ucp4C and was inhibited by guanosine diphosphate. UCP4C is required for development through the prepupal stages at low temperatures and may be an uncoupling protein.
authors.affiliation: Department of Biology, University of Padova, Padova, Italy.
authors.forename: Caterina
authors.initials: C
authors.lastname: Da-Ré
authors.forename: Cristiano
authors.initials: C
authors.lastname: De Pittà
authors.forename: Mauro A
authors.initials: MA
authors.lastname: Zordan
authors.forename: Giordano
authors.initials: G
authors.lastname: Teza
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Nestola
authors.forename: Massimo
authors.initials: M
authors.lastname: Zeviani
authors.forename: Rodolfo
authors.initials: R
authors.lastname: Costa
authors.forename: Paolo
authors.initials: P
authors.lastname: Bernardi
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003080:Cold Temperature; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000196:embryology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D055786:Gene Knockout Techniques; D006153:Guanosine Diphosphate / Q000494:pharmacology; D007814:Larva / Q000502:physiology*; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism*; D009840:Oligomycins / Q000494:pharmacology; D010101:Oxygen Consumption; D010168:Palmitates / Q000378:metabolism; D012119:Respiration / Q000187:drug effects*; D022722:Thermogenesis; D014475:Uncoupling Agents / Q000494:pharmacology
chemical_list: D029721:Drosophila Proteins; D004791:Enzyme Inhibitors; D026901:Membrane Transport Proteins; D009840:Oligomycins; D010168:Palmitates; C000594394:UCP4C protein, Drosophila; D014475:Uncoupling Agents; D006153:Guanosine Diphosphate