title: Selective homocysteine turn-on fluorescent probes and their bioimaging applications.
abstract: The design and development of new pyrene-based fluorescent probes, P-Hcy-1 and P-Hcy-2, which display selective fluorescence enhancements in response to homocysteine (Hcy), are described. The distinctly different fluorescence responses of P-Hcy-1 and P-Hcy-2 to Hcy vs. Cys are explained by theoretical calculations. Finally, the results of cell experiments show that these probes can be used to selectively detect Hcy in mammalian cells.
authors.affiliation: Department of Chemistry and Nano Science, Ewha Womans University, Seoul 120-750, Korea. jyoon@ewha.ac.kr.
authors.forename: Hye Yeon
authors.initials: HY
authors.lastname: Lee
authors.forename: Yoon Pyo
authors.initials: YP
authors.lastname: Choi
authors.forename: Sunkyung
authors.initials: S
authors.lastname: Kim
authors.forename: Taejin
authors.initials: T
authors.lastname: Yoon
authors.forename: Zhiqian
authors.initials: Z
authors.lastname: Guo
authors.forename: Songyi
authors.initials: S
authors.lastname: Lee
authors.forename: K M K
authors.initials: KM
authors.lastname: Swamy
authors.forename: Gyoungmi
authors.initials: G
authors.lastname: Kim
authors.forename: Jin Yong
authors.initials: JY
authors.lastname: Lee
authors.forename: Injae
authors.initials: I
authors.lastname: Shin
authors.forename: Juyoung
authors.initials: J
authors.lastname: Yoon
mesh_terms: D000818:Animals; D002460:Cell Line; D003952:Diagnostic Imaging / Q000379:methods*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006710:Homocysteine / Q000737:chemistry*; D006801:Humans
chemical_list: D005456:Fluorescent Dyes; D006710:Homocysteine