title: A surface-modified poly(ɛ-caprolactone) scaffold comprising variable nanosized surface-roughness using a plasma treatment.
abstract: Melt-plotted poly (ɛ-caprolactone) (PCL) has been widely applied in various tissue regenerations. However, its hydrophobic nature has hindered its usage in wider tissue engineering applications. In this study, we present the development of a porous and multilayered PCL scaffold, which shows outstanding hydrophilic properties and has a roughened surface consisting of homogeneously distributed nanosized pits. The scaffold was obtained using an innovative oxygen plasma treatment. This technology can induce variable nanoscale surface roughness, which is difficult from traditional plasma treatment. Osteoblast-like cells were cultured on the scaffolds and several cellular responses (cell viability, fluorescence images [live/dead cells, nucleus, and actin cytoskeleton], ALP activity, and calcium mineralization) were assessed for untreated PCL and conventionally plasma-treated PCL scaffolds. The data indicated that an appropriate roughness (654 ± 91 nm) of the PCL scaffold processed with the new plasma treatment induced more advantageous responses for the cells, compared with untreated scaffolds and traditional plasma-treated scaffolds.
authors.affiliation: Department of Bio-Mechatronic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University , Suwon, South Korea .
authors.forename: HoJun
authors.initials: H
authors.lastname: Jeon
authors.forename: Hyeongjin
authors.initials: H
authors.lastname: Lee
authors.forename: GeunHyung
authors.initials: G
authors.lastname: Kim
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D021621:Imaging, Three-Dimensional; D007211:Indoles / Q000378:metabolism; D018625:Microscopy, Atomic Force; D008903:Minerals / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D010590:Phalloidine / Q000378:metabolism; D056951:Photoelectron Spectroscopy; D058626:Plasma Gases / Q000494:pharmacology*; D011091:Polyesters / Q000494:pharmacology*; D016062:Porosity; D013314:Stress, Mechanical; D013499:Surface Properties; D013718:Tensile Strength / Q000187:drug effects; D054457:Tissue Scaffolds / Q000737:chemistry*; D014867:Water; D017155:Wettability
chemical_list: D007211:Indoles; D008903:Minerals; D058626:Plasma Gases; D011091:Polyesters; D014867:Water; D010590:Phalloidine; C016240:polycaprolactone; C007293:DAPI; D000469:Alkaline Phosphatase; D002118:Calcium