title: Breast cancer-associated protein--a novel binding partner of Mason-Pfizer monkey virus protease.
abstract: We identified breast cancer-associated protein (BCA3) as a novel binding partner of Mason-Pfizer monkey virus (MPMV) protease (PR). The interaction was confirmed by co-immunoprecipitation and immunocolocalization of MPMV PR and BCA3. Full-length but not C-terminally truncated BCA3 was incorporated into MPMV virions. We ruled out the potential role of the G-patch domain, a glycine-rich domain located at the C terminus of MPMV PR, in BCA3 interaction and virion incorporation. Expression of BCA3 did not affect MPMV particle release and proteolytic processing; however, it slightly increased MPMV infectivity.
authors.affiliation: Department of Biotechnology, Institute of Chemical Technology, Technická 5, 166 28 Prague, Czech Republic.|Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, v.v.i., IOCB & Gilead Research Center, Flemingovo nám. 2, 166 10 Prague, Czech Republic.
authors.forename: Michaela
authors.initials: M
authors.lastname: Rumlová
authors.affiliation: Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, v.v.i., IOCB & Gilead Research Center, Flemingovo nám. 2, 166 10 Prague, Czech Republic.
authors.forename: Ivana
authors.initials: I
authors.lastname: Křížová
authors.affiliation: Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, v.v.i., IOCB & Gilead Research Center, Flemingovo nám. 2, 166 10 Prague, Czech Republic.
authors.forename: Romana
authors.initials: R
authors.lastname: Hadravová
authors.affiliation: Department of Biochemistry and Microbiology, Institute of Chemical Technology, Technická 5, 166 28 Prague, Czech Republic.|Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, v.v.i., IOCB & Gilead Research Center, Flemingovo nám. 2, 166 10 Prague, Czech Republic.
authors.forename: Michal
authors.initials: M
authors.lastname: Doležal
authors.affiliation: Department of Biochemistry and Microbiology, Institute of Chemical Technology, Technická 5, 166 28 Prague, Czech Republic.|Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, v.v.i., IOCB & Gilead Research Center, Flemingovo nám. 2, 166 10 Prague, Czech Republic.
authors.forename: Karolína
authors.initials: K
authors.lastname: Strohalmová
authors.affiliation: Department of Biochemistry and Microbiology, Institute of Chemical Technology, Technická 5, 166 28 Prague, Czech Republic.|Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, v.v.i., IOCB & Gilead Research Center, Flemingovo nám. 2, 166 10 Prague, Czech Republic.
authors.forename: Alena
authors.initials: A
authors.lastname: Keprová
authors.affiliation: Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, v.v.i., IOCB & Gilead Research Center, Flemingovo nám. 2, 166 10 Prague, Czech Republic.
authors.forename: Iva
authors.initials: I
authors.lastname: Pichová
authors.affiliation: Department of Biochemistry and Microbiology, Institute of Chemical Technology, Technická 5, 166 28 Prague, Czech Republic.
authors.forename: Tomáš
authors.initials: T
authors.lastname: Ruml
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D010450:Endopeptidases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005260:Female; D057809:HEK293 Cells; D006801:Humans; D016093:Mason-Pfizer monkey virus / Q000201:enzymology* / Q000235:genetics; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
chemical_list: C471621:AKIP1 protein, human; D048868:Adaptor Proteins, Signal Transducing; D009687:Nuclear Proteins; D011994:Recombinant Proteins; D010450:Endopeptidases; C445563:Mason-Pfizer monkey virus protease