title: Lentivirus-mediated RNAi knockdown of LMP2A inhibits the growth of nasopharyngeal carcinoma cell line C666-1 in vitro.
abstract: Latent membrane protein 2A (LMP2A) is found to play a key role in the development of nasopharyngeal carcinoma (NPC). However, the role of LMP2A silencing in the inhibition of cell growth of NPC has not been clarified. In this study, we inhibited LMP2A gene expression by lentivirus-mediated RNAi, to explore the effects of LMP2A silencing on the growth of NPC cell line in vitro. A lentivirus-mediated RNAi technology was employed to specifically knock down the LMP2A gene in NPC cell line C666-1. Quantitative real-time polymerase chain reaction, Western blot, flow cytometry and colony formation assays were performed to evaluate the expression of LMP2A and biological behavior of cell line C666-1 in vitro. We successfully construct a highly efficient and stable lentivirus vector, which efficiently downregulate the expression of LMP2A gene in infected cell line C666-1. Down-regulation of the expression of LMP2A significantly inhibits the proliferation and colony formation of C666-1 cells. In addition, the specific down-regulation of LMP2A arrests cells in G0/G1 phase of cell cycle and increases apoptosis rate. Our findings suggest that lentivirus-mediated RNAi knockdown of LMP2A inhibits the growth of NPC cell line C666-1 in vitro, and LMP2A may be a potential target for gene therapy in treatment of NPC.
authors.affiliation: Department of Otolaryngology, Huadong Hospital, Fudan University, 221 Yan An Xi Road, Shanghai 200040, China.
authors.forename: Xinjiang
authors.initials: X
authors.lastname: Ying
authors.affiliation: Department of Otolaryngology, Huadong Hospital, Fudan University, 221 Yan An Xi Road, Shanghai 200040, China. Electronic address: zhangruxin@hotmail.com.
authors.forename: Ruxin
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Department of Otolaryngology, Huadong Hospital, Fudan University, 221 Yan An Xi Road, Shanghai 200040, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Otolaryngology, Huadong Hospital, Fudan University, 221 Yan An Xi Road, Shanghai 200040, China.
authors.forename: Yaoshu
authors.initials: Y
authors.lastname: Teng
mesh_terms: D017209:Apoptosis / Q000235:genetics*; D002277:Carcinoma; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D059585:G1 Phase Cell Cycle Checkpoints / Q000235:genetics; D015870:Gene Expression; D015316:Genetic Therapy / Q000379:methods; D006801:Humans; D016086:Lentivirus; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000235:genetics* / Q000473:pathology* / Q000628:therapy; D034622:RNA Interference; D034741:RNA, Small Interfering; D014763:Viral Matrix Proteins / Q000096:biosynthesis / Q000235:genetics*
chemical_list: C037662:EBV-associated membrane antigen, Epstein-Barr virus; D034741:RNA, Small Interfering; D014763:Viral Matrix Proteins
keywords: Latent membrane protein 2A; Lentivirus; Nasopharyngeal carcinoma; Proliferation; RNA interference