title: [Effect of co-expression of nicotinic acid phosphoribosyl transferase and pyruvate carboxylase on succinic acid production in Escherichia coli BA002].
abstract: Escherichia coli BA002, in which the ldhA and pflB genes are deleted, cannot utilize glucose anaerobically due to the inability to regenerate NAD+. To restore glucose utilization, overexpression of nicotinic acid phosphoribosyltransferase (NAPRTase) encoded by the pncB gene, a rate-limiting enzyme of NAD(H) synthesis pathway, resulted in a significant increase in cell mass and succinate production under anaerobic conditions. However, a high concentration of pyruvate was accumulated. Thus, co-expression of NAPRTase and the heterologous pyruvate carboxylase (PYC) of Lactococcus lactis subsp. cremoris NZ9000 in recombinant E. coli BA016 was investigated. Results in 3 L fermentor showed that OD600 is 4.64 and BA016 consumed 35.00 g/L glucose and produced 25.09 g/L succinate after 112 h under anaerobic conditions. Overexpression of pncB and pyc in BA016, the accumulation of pyruvic acid was further decreased, and the formation of succinic acid was further increased.
authors.forename: Weijia
authors.initials: W
authors.lastname: Cao
authors.forename: Dongmei
authors.initials: D
authors.lastname: Gou
authors.forename: Liya
authors.initials: L
authors.lastname: Liang
authors.forename: Rongming
authors.initials: R
authors.lastname: Liu
authors.forename: Kequan
authors.initials: K
authors.lastname: Chen
authors.forename: Jiangfeng
authors.initials: J
authors.lastname: Ma
authors.forename: Min
authors.initials: M
authors.lastname: Jiang
mesh_terms: D000693:Anaerobiosis; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism*; D005285:Fermentation; D005818:Genetic Engineering; D005947:Glucose / Q000378:metabolism; D007218:Industrial Microbiology; D013294:Lactococcus lactis / Q000201:enzymology; D009243:NAD / Q000378:metabolism; D010430:Pentosyltransferases / Q000096:biosynthesis* / Q000235:genetics; D011766:Pyruvate Carboxylase / Q000096:biosynthesis* / Q000235:genetics; D019802:Succinic Acid / Q000378:metabolism*
chemical_list: D009243:NAD; D019802:Succinic Acid; D010430:Pentosyltransferases; C021864:nicotinate phosphoribosyltransferase; D011766:Pyruvate Carboxylase; D005947:Glucose