title: Tumor-suppressing effects of miR-429 on human osteosarcoma.
abstract: Osteosarcoma is the most common primary bone tumor. Recent data indicated miRNAs may be involved in the pathogenesis of osteosarcoma, suggesting some novel targets for therapy. It is known that miR-429 is down-regulated and functions as a tumor suppressor by targeting c-myc and PLGG1 in gastric and breast cancer. However, the exact role of miR-429 in osteosarcoma remained unknown. In our study, we found MiR-429 was down-regulated in primary osteosarcoma lesion and osteosarcoma cell lines. Moreover, MiR-429 can inhibit the proliferation of osteosarcoma cell lines and induce more cell apoptosis. Also, we discovered MiR-429 plays a role in osteosarcoma by binding the 3'UTR of zinc finger E-box-binding homeobox 1 (ZEB1) mRNA, and that overexpression of ZEB1 could reverse the proliferation, subsequently blocking effect of miR-429. In conclusion, miR-429 serves as a tumor suppressor via interaction with ZEB1. Our finding may provide a new target for osteosarcoma therapy.
authors.affiliation: Department of Orthopedics of Jinling Hospital (Nanjing), School of Medicine, Southern Medical University, Guangzhou, 510515, People's Republic of China.
authors.forename: Xiaozhou
authors.initials: X
authors.lastname: Liu
authors.forename: Yunlai
authors.initials: Y
authors.lastname: Liu
authors.forename: Sujia
authors.initials: S
authors.lastname: Wu
authors.forename: Xin
authors.initials: X
authors.lastname: Shi
authors.forename: Lihong
authors.initials: L
authors.lastname: Li
authors.forename: Jianning
authors.initials: J
authors.lastname: Zhao
authors.forename: Haidong
authors.initials: H
authors.lastname: Xu
mesh_terms: D017209:Apoptosis / Q000235:genetics; D001483:Base Sequence; D001859:Bone Neoplasms / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D015536:Down-Regulation; D018398:Homeodomain Proteins / Q000235:genetics; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D012516:Osteosarcoma / Q000235:genetics* / Q000473:pathology; D014157:Transcription Factors / Q000235:genetics; D000071799:Zinc Finger E-box-Binding Homeobox 1
chemical_list: D018398:Homeodomain Proteins; C546363:MIRN429 microRNA, human; D035683:MicroRNAs; D014157:Transcription Factors; C486307:ZEB1 protein, human; D000071799:Zinc Finger E-box-Binding Homeobox 1