title: Comparison of miR-124-3p and miR-16 for early diagnosis of hemorrhagic and ischemic stroke.
abstract: BACKGROUND
  The present study aimed to investigate and compare plasma concentrations of miR-124-3p and miR-16 as diagnostic markers in acute stroke.
  
  
  METHODS
  miR-124-3p and miR-16 concentrations in 93 stroke patients were analyzed using real-time polymerase chain reaction. The primary outcome was the differentiation of hemorrhagic and ischemic stroke.
  
  
  RESULTS
  Of 93 patients, 74 (79.6%) were diagnosed as ischemic stroke (IS) and 19 (20.4%) were diagnosed as hemorrhagic stroke (HS). Median plasma 124-3p concentrations taken within 24h of symptom onset were higher in HS patients than that in IS patients (3.8×10(5) vs 2.0×10(5) copies/ml plasma, p=0.0109), while median miR-16 concentration in IS patients were higher than that in HS patients (1.6×10(9) vs 1.3×10(9) copies/ml plasma, p=0.0399). The odds ratio (OR) for discriminating HS and IS with miR-124-3p and miR-16 was 5.37 and 9.75 respectively.
  
  
  CONCLUSIONS
  Both miR-124-3p and miR-16 are diagnostic markers to discriminate HS and IS.
authors.affiliation: Accident and Emergency Medicine Academic Unit, The Chinese University of Hong Kong, Hong Kong.
authors.forename: Ling Yan
authors.initials: LY
authors.lastname: Leung
authors.affiliation: Accident and Emergency Medicine Academic Unit, The Chinese University of Hong Kong, Hong Kong.
authors.forename: Cangel Pui Yee
authors.initials: CP
authors.lastname: Chan
authors.affiliation: Accident and Emergency Medicine Academic Unit, The Chinese University of Hong Kong, Hong Kong.
authors.forename: Yuk Ki
authors.initials: YK
authors.lastname: Leung
authors.affiliation: Department of Emergency, The Second Affiliated Hospital of Guangzhou Medical University, Guangdong, China.
authors.forename: Hui Lin
authors.initials: HL
authors.lastname: Jiang
authors.affiliation: Department of Imaging and Interventional Radiology, The Chinese University of Hong Kong, Hong Kong.
authors.forename: Jill Morales
authors.initials: JM
authors.lastname: Abrigo
authors.affiliation: Department of Imaging and Interventional Radiology, The Chinese University of Hong Kong, Hong Kong.
authors.forename: De Feng
authors.initials: de F
authors.lastname: Wang
authors.affiliation: Accident and Emergency Department, Prince of Wales Hospital, Hong Kong.
authors.forename: Joseph Shun Hang
authors.initials: JS
authors.lastname: Chung
authors.affiliation: Accident and Emergency Medicine Academic Unit, The Chinese University of Hong Kong, Hong Kong. Electronic address: thrainer@cuhk.edu.hk.
authors.forename: Timothy Hudson
authors.initials: TH
authors.lastname: Rainer
authors.affiliation: Accident and Emergency Medicine Academic Unit, The Chinese University of Hong Kong, Hong Kong.
authors.forename: Colin Alexander
authors.initials: CA
authors.lastname: Graham
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017209:Apoptosis; D015415:Biomarkers / Q000097:blood; D001921:Brain / Q000378:metabolism / Q000473:pathology; D002545:Brain Ischemia / Q000150:complications*; D042241:Early Diagnosis; D005260:Female; D006801:Humans; D020300:Intracranial Hemorrhages / Q000150:complications*; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D009928:Organ Specificity; D020521:Stroke / Q000097:blood* / Q000150:complications / Q000175:diagnosis* / Q000473:pathology
chemical_list: D015415:Biomarkers; C509207:MIRN124 microRNA, human; C505362:MIRN16 microRNA, human; D035683:MicroRNAs
keywords: Diagnosis; Hemorrhagic stroke; Ischemic stroke; MicroRNA; Plasma