title: Oral delivery of plant-derived HIV-1 p24 antigen in low doses shows a superior priming effect in mice compared to high doses.
abstract: During early infection with human immunodeficiency virus type 1 (HIV-1), there is a rapid depletion of CD4(+) T-cells in the gut-associated lymphoid tissue (GALT) in the gastrointestinal tract. Therefore, immediate protection at these surfaces is of high priority for the development of an HIV-1 vaccine. Thus, transgenic plants expressing HIV-1 antigens, which are exposed to immune competent cells in the GALT during oral administration, can be interesting as potential vaccine candidates. In the present study, we used two HIV-1 p24 antigen-expressing transgenic plant systems, Arabidopsis thaliana and Daucus carota, in oral immunization experiments. Both transgenic plant systems showed a priming effect in mice and induced humoral immune responses, which could be detected as anti-p24-specific IgG in sera after an intramuscular p24 protein boost. Dose-dependent antigen analyses using transgenic A. thaliana indicated that low p24 antigen doses were superior to high p24 antigen doses.
authors.affiliation: Örebro Life Science Center, Örebro University, SE-70182 Örebro, Sweden; School of Science and Technology, Örebro University, SE-70182 Örebro, Sweden.
authors.forename: Ingrid
authors.initials: I
authors.lastname: Lindh
authors.affiliation: Swedish Institute for Communicable Disease Control (SMI), SE-17182 Stockholm, Sweden.
authors.forename: Andreas
authors.initials: A
authors.lastname: Bråve
authors.affiliation: Department of Microbiology, Tumor and Cell Biology, Karolinska Institute, SE-17177 Stockholm, Sweden.
authors.forename: David
authors.initials: D
authors.lastname: Hallengärd
authors.affiliation: Örebro Life Science Center, Örebro University, SE-70182 Örebro, Sweden; School of Science and Technology, Örebro University, SE-70182 Örebro, Sweden.
authors.forename: Ronza
authors.initials: R
authors.lastname: Hadad
authors.affiliation: Örebro Life Science Center, Örebro University, SE-70182 Örebro, Sweden; School of Science and Technology, Örebro University, SE-70182 Örebro, Sweden.
authors.forename: Irina
authors.initials: I
authors.lastname: Kalbina
authors.affiliation: Örebro Life Science Center, Örebro University, SE-70182 Örebro, Sweden; School of Science and Technology, Örebro University, SE-70182 Örebro, Sweden.
authors.forename: Åke
authors.initials: Å
authors.lastname: Strid
authors.affiliation: Örebro Life Science Center, Örebro University, SE-70182 Örebro, Sweden; Department of Laboratory Medicine, Örebro University Hospital, SE-70185 Örebro, Sweden. Electronic address: soren.andersson@orebroll.se.
authors.forename: Sören
authors.initials: S
authors.lastname: Andersson
mesh_terms: D016915:AIDS Vaccines / Q000276:immunology*; D000284:Administration, Oral; D000818:Animals; D017360:Arabidopsis; D018552:Daucus carota; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D015483:HIV Antibodies / Q000097:blood; D016655:HIV Core Protein p24 / Q000276:immunology*; D056724:Immunity, Humoral; D007117:Immunization, Secondary; D007074:Immunoglobulin G / Q000097:blood; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008822:Mice, Transgenic; D030821:Plants, Genetically Modified
chemical_list: D016915:AIDS Vaccines; D015483:HIV Antibodies; D016655:HIV Core Protein p24; D007074:Immunoglobulin G
keywords: Arabidopsis thaliana; Carrot; Edible vaccine; HIV-1 p24; Oral immunization; Transgenic plants