title: Advanced nitrogen removal from landfill leachate using real-time controlled three-stage sequence batch reactor (SBR) system.
abstract: A three-stage sequencing batch reactor (SBR), comprising pretreating SBR (SBRpre), nitritation SBR (SBRni), and anaerobic ammonium oxidation (Anammox) SBR (SBRana), was developed for the nitrogen removal from mature landfill leachate. The concentrations of ammonia and chemical oxygen demand (COD) in the leachate were 2000 ± 100 and 2200 ± 200 mg/L, respectively. About 100mg/L of organic substance was removed from SBRpre to reduce the negative effect on the Anammox process under real-time control. After acclimation for 40 days, the nitrite to nitrogen oxide ratio (NO2(-)/NOx) in SBRni was above 0.95. The nitrogen removal efficiency reached 90% in SBRana, and nitrogen load rate and nitrogen removal rate were 0.81 and 0.76 kg N/(m(3)d), respectively. The continuous filling process was used to avoid the nitrite inhibition on the Anammox activity. The quantitative PCR analysis of Anammox indicated the average Anammox gene ratio increased from 0.23% to 4.77% after 220 days operation.
authors.affiliation: Engineering Research Center of Beijing, Beijing University of Technology, Beijing, China. Electronic address: miaolei2819@emails.bjut.edu.cn.
authors.forename: Lei
authors.initials: L
authors.lastname: Miao
authors.affiliation: Engineering Research Center of Beijing, Beijing University of Technology, Beijing, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Wang
authors.affiliation: Engineering Research Center of Beijing, Beijing University of Technology, Beijing, China. Electronic address: wsy@bjut.edu.cn.
authors.forename: Shuying
authors.initials: S
authors.lastname: Wang
authors.affiliation: Engineering Research Center of Beijing, Beijing University of Technology, Beijing, China.
authors.forename: Rulong
authors.initials: R
authors.lastname: Zhu
authors.affiliation: Engineering Research Center of Beijing, Beijing University of Technology, Beijing, China.
authors.forename: Baikun
authors.initials: B
authors.lastname: Li
authors.affiliation: Engineering Research Center of Beijing, Beijing University of Technology, Beijing, China.
authors.forename: Yongzhen
authors.initials: Y
authors.lastname: Peng
authors.affiliation: Engineering Research Center of Beijing, Beijing University of Technology, Beijing, China.
authors.forename: Dongchen
authors.initials: D
authors.lastname: Weng
mesh_terms: D000064:Acclimatization; D000641:Ammonia / Q000378:metabolism; D000693:Anaerobiosis; D001419:Bacteria / Q000378:metabolism; D061249:Batch Cell Culture Techniques / Q000295:instrumentation*; D001673:Biodegradation, Environmental; D057919:Biological Oxygen Demand Analysis; D019149:Bioreactors / Q000382:microbiology; D003199:Computer Systems; D058440:Denitrification; D006863:Hydrogen-Ion Concentration; D009584:Nitrogen / Q000302:isolation & purification*; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000737:chemistry*; D018508:Water Purification / Q000295:instrumentation*
chemical_list: D014874:Water Pollutants, Chemical; D000641:Ammonia; D009584:Nitrogen; D010100:Oxygen
keywords: Anammox; Landfill leachate; Nitrogen removal; Real-time control; SBR