title: [Effect of a hypocaloric diet in the oxidative stress in obese subjects without prescription of exercise and antioxidants].
abstract: OBJECTIVE
  Obesity is characterized by a generalized increase of adipose tissue, high production of adipocytokines and presence of oxidative systemic stress. The objective of this study was to evaluate the changes generated in the oxidative stress and anthropometric parameters in obese subjects by the prescription of a hypocaloric diet in combination with moderate aerobic exercise and supplementation with antioxidants.
  
  
  METHODS
  Oxidative damage was determined in the plasma from 30 normal weight and 30 obese subjects. Three groups of treatment were established: Hypocaloric diet (HD), HD plus moderate aerobic exercise (HDE) and HDE plus antioxidants (DHEA). Biomarkers of oxidative stress (thiobarbituric acid reactive substances [TBARS], carbonyl groups, dityrosine) and anthropometric parameters were determined.
  
  
  RESULTS
  Higher values of biomarkers of oxidative damage were observed in obese (TBARS 13.74 ± 1.2 μM; carbonyl groups 0.89 ± 0.04 nmol of osazone/mg of protein; dityrosine 478.9 ± 27.4 RFU/mg of protein) in comparison to normal weight subjects (TBARS 7.08 ± 0.8 μM; carbonyl groups 0.65 ± 0.04 nmol of osazone/mg of protein; dityrosine 126.3 ± 12.6 RFU/mg of protein), thus showing the presence of an oxidative damage. The prescription of HD decreased the oxidative damage and anthropometric parameters in the obese subjects. We did not observe additional benefit effects on these determinations with HDE or HDEA treatments.
  
  
  CONCLUSIONS
  We demonstrated that an HD decreases the oxidative damage in obese subjects. Oxidative stress is an important factor in the development of comorbidity in obesity. Therefore, the prescription of a HD could be a key issue in the treatment of the disease.
authors.affiliation: Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional, Casco de Santo Tomás, México D. F., México.
authors.forename: Liliana
authors.initials: L
authors.lastname: Gutiérrez
authors.affiliation: Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional, Casco de Santo Tomás, México D. F., México. Electronic address: rubeng26@excite.com.
authors.forename: José R
authors.initials: JR
authors.lastname: García
authors.affiliation: Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional, Casco de Santo Tomás, México D. F., México.
authors.forename: María de Jesús
authors.initials: Mde J
authors.lastname: Rincón
authors.affiliation: Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional, Casco de Santo Tomás, México D. F., México.
authors.forename: Guillermo M
authors.initials: GM
authors.lastname: Ceballos
authors.affiliation: Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional, Casco de Santo Tomás, México D. F., México.
authors.forename: Ivonne M
authors.initials: IM
authors.lastname: Olivares
mesh_terms: D000328:Adult; D000886:Anthropometry; D000975:Antioxidants / Q000627:therapeutic use*; D015415:Biomarkers; D031204:Caloric Restriction; D003131:Combined Modality Therapy; D004038:Diet, Reducing; D005081:Exercise Therapy; D005260:Female; D006801:Humans; D008297:Male; D008903:Minerals / Q000627:therapeutic use; D009765:Obesity / Q000097:blood / Q000178:diet therapy* / Q000378:metabolism / Q000628:therapy; D018384:Oxidative Stress / Q000187:drug effects; D050050:Protein Carbonylation; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D016896:Treatment Outcome; D014443:Tyrosine / Q000031:analogs & derivatives / Q000097:blood; D014815:Vitamins / Q000627:therapeutic use; D055815:Young Adult
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D008903:Minerals; D017392:Thiobarbituric Acid Reactive Substances; D014815:Vitamins; D014443:Tyrosine; C007543:dityrosine
keywords: Antioxidantes; Antioxidants; Body mass index; Daño oxidativo; Dieta hipocalórica; Estrés oxidativo; Hypocaloric diet; Obesidad; Obesity; Oxidative damage; Oxidative stress; Índice de masa corporal