title: From zebrafish heart jogging genes to mouse and human orthologs: using Gene Ontology to investigate mammalian heart development.
abstract: For the majority of organs in developing vertebrate embryos, left-right asymmetry is controlled by a ciliated region; the left-right organizer node in the mouse and human, and the Kuppfer's vesicle in the zebrafish. In the zebrafish, laterality cues from the Kuppfer's vesicle determine asymmetry in the developing heart, the direction of 'heart jogging' and the direction of 'heart looping'.  'Heart jogging' is the term given to the process by which the symmetrical zebrafish heart tube is displaced relative to the dorsal midline, with a leftward 'jog'. Heart jogging is not considered to occur in mammals, although a leftward shift of the developing mouse caudal heart does occur prior to looping, which may be analogous to zebrafish heart jogging. Previous studies have characterized 30 genes involved in zebrafish heart jogging, the majority of which have well defined orthologs in mouse and human and many of these orthologs have been associated with early mammalian heart development.    We undertook manual curation of a specific set of genes associated with heart development and we describe the use of Gene Ontology term enrichment analyses to examine the cellular processes associated with heart jogging.  We found that the human, mouse and zebrafish 'heart jogging orthologs' are involved in similar organ developmental processes across the three species, such as heart, kidney and nervous system development, as well as more specific cellular processes such as cilium development and function. The results of these analyses are consistent with a role for cilia in the determination of left-right asymmetry of many internal organs, in addition to their known role in zebrafish heart jogging.    This study highlights the importance of model organisms in the study of human heart development, and emphasises both the conservation and divergence of developmental processes across vertebrates, as well as the limitations of this approach.
authors.affiliation: Cardiovascular GO Annotation Initiative, Centre for Cardiovascular Genetics, Institute of Cardiovascular Science, University College London, London, WC1E 6JF, UK.
authors.forename: Varsha K
authors.initials: VK
authors.lastname: Khodiyar
authors.affiliation: The Zebrafish Model Organism Database, University of Oregon, Eugene, OR, 97403-5291, USA.
authors.forename: Doug
authors.initials: D
authors.lastname: Howe
authors.affiliation: Cardiovascular GO Annotation Initiative, Centre for Cardiovascular Genetics, Institute of Cardiovascular Science, University College London, London, WC1E 6JF, UK.
authors.forename: Philippa J
authors.initials: PJ
authors.lastname: Talmud
authors.affiliation: Centre for Metabolism and Experimental Therapeutics, University College London, London, WC1E 6JF, UK.
authors.forename: Ross
authors.initials: R
authors.lastname: Breckenridge
authors.affiliation: Cardiovascular GO Annotation Initiative, Centre for Cardiovascular Genetics, Institute of Cardiovascular Science, University College London, London, WC1E 6JF, UK.
authors.forename: Ruth C
authors.initials: RC
authors.lastname: Lovering