title: Magnetic nanoparticles based dispersive micro-solid-phase extraction as a novel technique for coextraction of acidic and basic drugs from biological fluids and waste water.
abstract: The coextraction of acidic and basic drugs from different samples is a considerable and disputable concept in sample preparation strategies. In this study, for the first time, simultaneous extraction of acidic and basic drugs with magnetic nanoparticles based dispersive micro-solid phase extraction followed by high performance liquid chromatography-ultraviolet detection was introduced. Cetyltrimethyl ammonium bromide-coated Fe3O4@decanoic acid as an efficient sorbent was successfully applied to adsorb diclofenac (DIC) as an acidic and diphenhydramine (DPH) as a basic model compound. First, appropriate amount of synthetic Fe3O4@decanoic acid nanoparticles was added to aqueous solution of drugs. After adjusting the pH of the solution, cetyltrimethyl ammonium bromide (CTAB) was added to the mixture being stirred at a constant rate. After the adsorption of drugs and decantation of supernatant with a magnetic field, the sorbent was eluted with methanol by fierce vortex. The parameters affecting the extraction efficiency were optimized and obtained as: pH of the sample=9, concentration of CTAB=0.2mmolL(-1), amount of sorbent=10mg, extraction time=5min, no salt addition to sample, type and volume of the eluent=50μL methanol, and desorption time=1min. Under the optimum conditions detection limits and linear dynamic ranges were achieved in the range of 1.8-3.0, 5-1500μgL(-1) for DPH and 1.5-3.5, 5-1500μgL(-1) for DIC, respectively. The percent of extraction recovery and relative standard deviations (n=5) were in the range of 47.3-60, 5.2-9.0 for DPH and 64-76.7, 5.1-5.8 for DIC, respectively. Ultimately, the applicability of the method was successfully confirmed by the extraction and determination of DIC and DPH in human urine, plasma and waste water samples in the range of microgram per liter and satisfactory results were obtained.
authors.affiliation: Faculty of Chemistry, Shahid Beheshti University, G.C., Evin, Tehran, Iran.
authors.forename: Ali Akbar
authors.initials: AA
authors.lastname: Asgharinezhad
authors.affiliation: Faculty of Chemistry, Shahid Beheshti University, G.C., Evin, Tehran, Iran.
authors.forename: Narges
authors.initials: N
authors.lastname: Mollazadeh
authors.affiliation: Faculty of Chemistry, Shahid Beheshti University, G.C., Evin, Tehran, Iran. Electronic address: h-ebrahim@sbu.ac.ir.
authors.forename: Homeira
authors.initials: H
authors.lastname: Ebrahimzadeh
authors.affiliation: Faculty of Chemistry, Shahid Beheshti University, G.C., Evin, Tehran, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Mirbabaei
authors.affiliation: Faculty of Chemistry, Shahid Beheshti University, G.C., Evin, Tehran, Iran.
authors.forename: Nafiseh
authors.initials: N
authors.lastname: Shekari
mesh_terms: D000327:Adsorption; D001774:Blood Chemical Analysis / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D019815:Oxalic Acid / Q000737:chemistry*; D052617:Solid Phase Microextraction / Q000379:methods*; D016482:Urinalysis / Q000379:methods*; D062065:Wastewater / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D058185:Magnetite Nanoparticles; D019815:Oxalic Acid; D062065:Wastewater; D014874:Water Pollutants, Chemical
keywords: Cetyltrimethyl ammonium bromide-coated Fe(3)O(4)@decanoic acid; Coextraction; Diclofenac; Diphenhydramine; Dispersive micro-solid phase extraction