title: The real-time quantification of autofluorescence spectrum shape for the monitoring of mitochondrial metabolism.
abstract: The cellular proportion of free and protein-bound NADH complexes is increasingly recognized as a metabolic indicator and biomarker. Because free and bound forms exhibit different fluorescence spectra, we consider whether autofluorescence shape sufficiently correlates with mitochondrial metabolism to be useful for monitoring in cellular suspensions. Several computational approaches for rapidly quantifying spectrum shape are used to detect Saccharomyces cereviseae response to oxygenation, and to the addition of mitochondrial functional modifiers and metabolic substrates. Observed changes appear consistent with previous studies probing free/protein-bound proportions, making this a potentially useful approach for the real-time monitoring of metabolism. (© 2015 WILEY-VCH Verlag GmbH &Co. KGaA, Weinheim).
authors.affiliation: Department of Physics, Miami University, Oxford, OH, USA.
authors.forename: Zac
authors.initials: Z
authors.lastname: Long
authors.forename: Jeff
authors.initials: J
authors.lastname: Maltas
authors.forename: Michael C
authors.initials: MC
authors.lastname: Zatt
authors.forename: Jun
authors.initials: J
authors.lastname: Cheng
authors.forename: Erik J
authors.initials: EJ
authors.lastname: Alquist
authors.forename: Alex
authors.initials: A
authors.lastname: Brest
authors.forename: Paul
authors.initials: P
authors.lastname: Urayama
mesh_terms: D000431:Ethanol / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology; D006358:Hot Temperature; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013997:Time Factors
chemical_list: D000431:Ethanol; D005947:Glucose; D010100:Oxygen
keywords: NADH; autofluorescence; fluorescence spectroscopy; mitochondrial metabolism