title: Contribution of CDKAL1 rs7756992 and IGF2BP2 rs4402960 polymorphisms in type 2 diabetes, diabetic complications, obesity risk and hypertension in the Tunisian population.
abstract: BACKGROUND
  The insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2) and the cyclin-dependent kinase 5 regulatory subunit-associated protein 1-like 1 (CDKAL1) identified through genome-wide association (GWA) studies have been shown to be associated with Type 2 diabetes in various ethnic groups. In this study, we investigated the association of the rs7756992 of CDKAL1 and the rs4402960 of IGF2BP2 with Type 2 diabetes, diabetic complications (nephropathy, retinopathy and cardiovascular disease), obesity and hypertension in a Tunisian population.
  
  
  METHODS
  A case-control association study including 200 Type 2 diabetes Tunisian patients (World Health Organization criteria) and 208 controls (age ≥40; fasting plasma glucose <6.1 mmol/L; without first degree family history of diabetes) has been performed. Other parameters such as diabetic nephropathy, diabetic retinopathy, cardiovascular disease, overweight/obesity and hypertension have been also collected. Genotyping was performed using TaqMan technology.
  
  
  RESULTS
  A significant association between the rs4402960 and Type 2 diabetes (OR = 1.86, 95% CI = 1.34-2.58, P < 10(-4) ) has been found. Overweight/obese subjects bearing the T-allele have an increased risk to develop Type 2 diabetes (OR = 2.06, 95% CI = 1.40-3.03, P < 10(-4) ). Furthermore, the rs7756992 was found to be associated with the reduced risk of diabetic nephropathy in patients with diabetes (OR = 0.44, 95% CI = 0.27-0.73, P = 0.001).
  
  
  CONCLUSIONS
  The present study confirms that the rs4402960 of IGF2BP2 gene is a strong candidate for Type 2 diabetes susceptibility and overweight/obesity risk in the Tunisian population. Interestingly, our data suggest that the rs7756992 of CDKAL1 gene have a protective effect against diabetic nephropathy.
authors.affiliation: Biomedical Genomics and Oncogenetics Laboratory (LR 11 IPT 05), Institut Pasteur de Tunis, Université El Manar de Tunis, Tunis, Tunisia.
authors.forename: Khaled
authors.initials: K
authors.lastname: Lasram
authors.forename: Nizar
authors.initials: N
authors.lastname: Ben Halim
authors.forename: Houda
authors.initials: H
authors.lastname: Benrahma
authors.forename: Sounnia
authors.initials: S
authors.lastname: Mediene-Benchekor
authors.forename: Imen
authors.initials: I
authors.lastname: Arfa
authors.forename: Sana
authors.initials: S
authors.lastname: Hsouna
authors.forename: Rym
authors.initials: R
authors.lastname: Kefi
authors.forename: Henda
authors.initials: H
authors.lastname: Jamoussi
authors.forename: Slim
authors.initials: S
authors.lastname: Ben Ammar
authors.forename: Sonia
authors.initials: S
authors.lastname: Bahri
authors.forename: Abdelmajid
authors.initials: A
authors.lastname: Abid
authors.forename: Soraya
authors.initials: S
authors.lastname: Benhamamouch
authors.forename: Abdelhamid
authors.initials: A
authors.lastname: Barakat
authors.forename: Sonia
authors.initials: S
authors.lastname: Abdelhak
mesh_terms: D002318:Cardiovascular Diseases / Q000209:etiology; D016022:Case-Control Studies; D051360:Cyclin-Dependent Kinase 5 / Q000235:genetics*; D048909:Diabetes Complications / Q000453:epidemiology / Q000209:etiology*; D003924:Diabetes Mellitus, Type 2 / Q000150:complications* / Q000453:epidemiology / Q000503:physiopathology; D003928:Diabetic Nephropathies / Q000453:epidemiology / Q000209:etiology; D003930:Diabetic Retinopathy / Q000453:epidemiology / Q000209:etiology; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D006973:Hypertension / Q000453:epidemiology / Q000209:etiology*; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000453:epidemiology / Q000209:etiology*; D011110:Polymorphism, Genetic / Q000235:genetics*; D011379:Prognosis; D016601:RNA-Binding Proteins / Q000235:genetics*; D014416:Tunisia / Q000453:epidemiology; D012359:tRNA Methyltransferases
chemical_list: C498779:IGF2BP2 protein, human; D016601:RNA-Binding Proteins; D012359:tRNA Methyltransferases; D051360:Cyclin-Dependent Kinase 5; C525110:CDKAL1 protein, human
keywords: CDKAL1; IGF2BP2; Tunisian population; Type 2 diabetes; genetic association; 关键词：CDKAL1
  ，遗传相关性，IGF2BP2
  ，突尼斯人群，2型糖尿病