title: The prevalence of Y chromosome microdeletions in Pakistani infertile men.
abstract: BACKGROUND
  Microdeletions of the azoospermia factor locus of the long arm of Y chromosome are an etiological factor of severe oligozoospermia or azoospermia.
  
  
  OBJECTIVE
  The aim of this study was to investigate the prevalence of Y-chromosome microdeletions in AZF region and their role in infertility in Pakistani population.
  
  
  METHODS
  The type of deletions in AZF locus were detected in infertile men (n=113) and the association of Y chromosome microdeletions with male infertility was assessed by including men (50) with normal karyotype and having children. Y chromosome microdeletions were detected by multiplex PCR using 10 sequence tagged sites namely sY81, sY130, sY141, sY142, sY155, sY157, sY160, sY182, sY231, and sY202 that covered all three regions of AZF.
  
  
  RESULTS
  Individuals with severe oligozoospermia showed 2.86% deletion frequency in AZFc region as compared to azoospermic males (5.5%).
  
  
  CONCLUSIONS
  The results of our study showed that deletions in Y chromosome are not playing major part in male infertility. Moreover, multiplex-PCR strategy might preferably be employed for the detection of Y chromosome microdeletions allied to male infertility.
authors.affiliation: Health Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan.
authors.forename: Rubina
authors.initials: R
authors.lastname: Tabassum Siddiqui
authors.affiliation: Institute of Biochemistry and Biotechnology, University of the Punjab, Quaid-e-Azam Campus, Lahore, Pakistan.
authors.forename: Nosheen
authors.initials: N
authors.lastname: Mujtaba
authors.affiliation: Institute of Biochemistry and Biotechnology, University of the Punjab, Quaid-e-Azam Campus, Lahore, Pakistan.
authors.forename: Mamoona
authors.initials: M
authors.lastname: Naz
keywords: Azoospermia; Male infertility; Y Chromosome deletions