title: Quantitative analysis and pharmacokinetics study of tigecycline in human serum using a validated sensitive liquid chromatography with tandem mass spectrometry method.
abstract: Tigecycline, a novel intravenously administered glycylcycline antibiotic, currently plays a key role in the management of complicated multiorganism infections. However, current liquid chromatography with tandem mass spectrometry methods briefly describe parameters and the only reported internal standard was sometimes difficult to obtain. In our study, an updated liquid chromatography with tandem mass spectrometry method for the quantitative analysis of tigecycline in human serum was developed. Sample preparation involved precipitation with 20% trichloroacetic acid. Chromatographic separation of tigecycline and tetracycline (internal standard) was achieved on a Hypersil GOLD C18 column using gradient elution. The selected reaction monitoring transitions were performed at m/z 586.1→513.2 for tigecycline and m/z 445.1→410.2 for tetracycline. The assay was linear over the concentration range of 5-2000 ng/mL. The intra- and interday precisions at three concentration levels (10, 100, and 1600 ng/mL) were <15% and their accuracies were within the range of 95.1-106.1%. The mean recovery ranged from 94.3 to 105.6% and the matrix effect from 92.1 to 97.6%. Tigecycline was stable under all tested conditions. This validated method was successfully applied to a pharmacokinetic study in critically ill patients. The data demonstrated that our method allows quantification of tigecycline in serum in a quick and reliable manner for widespread application.
authors.affiliation: Department of Pharmacy, The First Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an, China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Xie
authors.forename: Taotao
authors.initials: T
authors.lastname: Wang
authors.forename: Xue
authors.initials: X
authors.lastname: Wang
authors.forename: Xiaoliang
authors.initials: X
authors.lastname: Cheng
authors.forename: Haiyan
authors.initials: H
authors.lastname: Dong
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Zheng
authors.forename: Liang
authors.initials: L
authors.lastname: Zhou
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Xing
authors.forename: Yalin
authors.initials: Y
authors.lastname: Dong
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D008911:Minocycline / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*; D000078304:Tigecycline
chemical_list: D000078304:Tigecycline; D008911:Minocycline
keywords: Intensive care medicine; Pharmacokinetics; Tetracycline; Tigecycline; liquid chromatography with tandem mass spectrometry