title: One-step reduction and PEGylation of graphene oxide for photothermally controlled drug delivery.
abstract: Here, we developed one-step green reduction and PEGylation of nanosized graphene oxide (NGO) to obtain NrGO/PEG as a photothermally controllable drug delivery system. NrGO/PEG was synthesized by bathing methoxypolyethylene glycol amine (mPEG-NH2) and NGO at 90 °C for 24 h. The NrGO/PEG kept water stability for at least two months, and had ~14-fold increment in near-infrared (NIR) absorbance and ~2-fold increment in resveratrol (RV) loading over the unreduced NGO/PEG via π-π and hydrophobic interactions. Exposure of 4T1 cells to NrGO/PEG for 2 h showed 53.6% uptake ratio, and localization of NrGO/PEG in lysosomes instead of mitochondria. NIR irradiation (808 nm laser at 0.6 W/cm(2)) for 3 min potently enhanced RV release from NrGO/PEG-RV and the cytotoxicity of NrGO/PEG-RV against 4T1 cells, including decrease of cell viability, loss of mitochondrial membrane potential (ΔΨm) and cell apoptosis. Finally, NIR irradiation dramatically enhanced the efficacy of NrGO/PEG-RV in suppressing tumor growth in animal tumor models, further proving the remarkable synergistic action between photothermal effect of NrGO/PEG and RV loaded on NrGO/PEG.
authors.affiliation: MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China.
authors.forename: Jingqin
authors.initials: J
authors.lastname: Chen
authors.affiliation: MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Liu
authors.affiliation: MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China.
authors.forename: Chubiao
authors.initials: C
authors.lastname: Zhao
authors.affiliation: MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China.
authors.forename: Guiqi
authors.initials: G
authors.lastname: Qin
authors.affiliation: MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China.
authors.forename: Gaina
authors.initials: G
authors.lastname: Xi
authors.affiliation: MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China.
authors.forename: Tan
authors.initials: T
authors.lastname: Li
authors.affiliation: Department of Pain Management, The First Affiliated Hospital of Jinan University, Guangzhou 510632, China. Electronic address: txp2938@jnu.edu.cn.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Wang
authors.affiliation: MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China. Electronic address: chentsh@scnu.edu.cn.
authors.forename: Tongsheng
authors.initials: T
authors.lastname: Chen
mesh_terms: D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D016503:Drug Delivery Systems / Q000379:methods*; D006108:Graphite / Q000737:chemistry*; D006358:Hot Temperature; D057927:Hydrophobic and Hydrophilic Interactions; D007259:Infrared Rays; D008247:Lysosomes / Q000187:drug effects / Q000528:radiation effects; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects / Q000528:radiation effects; D051379:Mice; D049329:Nanostructures / Q000737:chemistry; D010087:Oxides / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology
chemical_list: D016588:Anticarcinogenic Agents; D010087:Oxides; D013267:Stilbenes; D011092:Polyethylene Glycols; D006108:Graphite; C028210:monomethoxypolyethylene glycol; D000077185:Resveratrol
keywords: Green reduction; Nanosized graphene oxide; Near-infrared irradiation; PEGylation; Photothermal effect; Resveratrol