title: Affinity tags in protein purification and peptide enrichment: an overview.
abstract: The reversible interaction between an affinity ligand and a complementary receptor has been widely explored in purification systems for several biomolecules. The development of tailored affinity ligands highly specific towards particular target biomolecules is one of the options in affinity purification systems. However, both genetic and chemical modifications on proteins and peptides widen the application of affinity ligand-tag receptor pairs towards universal capture and purification strategies. In particular, this chapter will focus on two case studies highly relevant for biotechnology and biomedical areas, namely, the affinity tags and receptors employed on the production of recombinant fusion proteins and the chemical modification of phosphate groups on proteins and peptides and the subsequent specific capture and enrichment, a mandatory step before further proteomic analysis.
authors.affiliation: REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Caparica, Portugal.
authors.forename: Ana Sofia
authors.initials: AS
authors.lastname: Pina
authors.forename: Iris L
authors.initials: IL
authors.lastname: Batalha
authors.forename: Ana Cecília A
authors.initials: AC
authors.lastname: Roque
mesh_terms: D000345:Affinity Labels; D000595:Amino Acid Sequence; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry*; D011506:Proteins / Q000302:isolation & purification*
chemical_list: D000345:Affinity Labels; D010455:Peptides; D011506:Proteins