title: Presence of heat shock protein 70 in secondary lymphoid tissue correlates with stroke prognosis.
abstract: Heat shock protein 70 (Hsp-70) can act as a danger signal and activate immune responses. We studied the presence of Hsp-70 in lymphoid tissue and plasma of acute stroke patients and asymptomatic controls free of neurological disease. Immunofluorescence, Western blotting, qRT-PCR and flow cytometry studies were performed. Plasma Hsp-70 concentration at day 7 was similar in patients and controls, whereas patients disclosed stronger immunoreactivity to Hsp-70 in lymphoid tissue than controls. Most Hsp-70+ cells were antigen presenting cells located in T cell zones. Stronger immunoreactivity to Hsp-70 was associated with smaller infarctions and better functional outcome.
authors.affiliation: Functional Unit of Cerebrovascular Diseases, Hospital Clínic, Barcelona, Spain.
authors.forename: Manuel
authors.initials: M
authors.lastname: Gómez-Choco
authors.affiliation: Department of Brain Ischemia and Neurodegeneration, Institute for Biomedical Research of Barcelona (IIBB), Spanish Research Council (CSIC), Barcelona, Spain; August Pi i Sunyer Biomedical Research Institute (IDIBAPS), Barcelona, Spain.
authors.forename: Cedric
authors.initials: C
authors.lastname: Doucerain
authors.affiliation: Functional Unit of Cerebrovascular Diseases, Hospital Clínic, Barcelona, Spain; August Pi i Sunyer Biomedical Research Institute (IDIBAPS), Barcelona, Spain.
authors.forename: Xabier
authors.initials: X
authors.lastname: Urra
authors.affiliation: Department of Brain Ischemia and Neurodegeneration, Institute for Biomedical Research of Barcelona (IIBB), Spanish Research Council (CSIC), Barcelona, Spain; August Pi i Sunyer Biomedical Research Institute (IDIBAPS), Barcelona, Spain.
authors.forename: Anna M
authors.initials: AM
authors.lastname: Planas
authors.affiliation: Functional Unit of Cerebrovascular Diseases, Hospital Clínic, Barcelona, Spain; August Pi i Sunyer Biomedical Research Institute (IDIBAPS), Barcelona, Spain; School of Medicine, University of Barcelona, Spain. Electronic address: achamorro@ub.edu.
authors.forename: Angel
authors.initials: A
authors.lastname: Chamorro
mesh_terms: D000368:Aged; D015415:Biomarkers / Q000032:analysis* / Q000378:metabolism; D015153:Blotting, Western; D005260:Female; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D018840:HSP70 Heat-Shock Proteins / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D008221:Lymphoid Tissue / Q000276:immunology / Q000378:metabolism*; D008297:Male; D011379:Prognosis; D060888:Real-Time Polymerase Chain Reaction; D020127:Recovery of Function / Q000276:immunology; D020521:Stroke / Q000276:immunology / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D018840:HSP70 Heat-Shock Proteins
keywords: Acute stroke; Antigen presentation; Heat shock protein; Lymphoid tissue; Neuroimmunology