title: Cross talk between angiotensin-(1-7)/Mas axis and sirtuins in adipose tissue and metabolism of high-fat feed mice.
abstract: Angiotensin-(1-7) and resveratrol have been described as new potential therapeutic tools on treating and preventing metabolic disorders. In the present study we aimed to evaluate the effect of an oral formulation of angiotensin-(1-7) [Ang-(1-7)] included in HPB-cyclodextrin and resveratrol (RSV), in modulation of sirtuin and renin-angiotensin system (RAS) in adipose tissue of mice treated with a high-fat diet (HFD). We observed that HFD+Ang-(1-7) and HFD+RSV groups presented marked decrease in the adipose tissue mass. Furthermore, these animals showed improved insulin-sensitivity and glucose tolerance as well as lower plasma levels of fasting glucose and lipids. The RT-PCR analysis revealed decreased expression of ACE and an increase of ACE2 [Ang-(1-7) marker] in group treated with resveratrol and also an increased expression of SIRT1 in groups that received Ang-(1-7). We showed for the first time that improved metabolic profile is associated with increased expression of GLUT4 and high expression of AMPK/FOXO1/PPAR-γ pathway in adipose-tissue. Finally, adipocyte primary cell-culture incubated with and without sirtuin and Ang-(1-7)/Mas antagonists pointed out for a cross-talking between RAS and sirtuins. We conclude that oral administration of Ang-(1-7) and RSV improved metabolic profile through a cross-modulation between RAS and Sirtuins.
authors.affiliation: Laboratory of Health Science, Postgraduate Program in Health Sciences, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais, Brazil.
authors.forename: João Marcus
authors.initials: JM
authors.lastname: Oliveira Andrade
authors.affiliation: Laboratory of Health Science, Postgraduate Program in Health Sciences, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais, Brazil.
authors.forename: Alanna Fernandes
authors.initials: AF
authors.lastname: Paraíso
authors.affiliation: Laboratory of Microbiology, Postgraduate Program in Microbiology, Universidade Federal de Minas Gerais, Minas Gerais, Brazil.
authors.forename: Zélia Menezes
authors.initials: ZM
authors.lastname: Garcia
authors.affiliation: Department of Basic Nursing, Nutrition School, Universidade Federal de Minas Gerais, Minas Gerais, Brazil.
authors.forename: Adaliene Versiani Matos
authors.initials: AV
authors.lastname: Ferreira
authors.affiliation: Department of Chemistry, Universidade Federal de Minas Gerais, Minas Gerais, Brazil.
authors.forename: Ruben D M
authors.initials: RD
authors.lastname: Sinisterra
authors.affiliation: Department of Chemistry, Universidade Federal de Minas Gerais, Minas Gerais, Brazil.
authors.forename: Frederico B
authors.initials: FB
authors.lastname: Sousa
authors.affiliation: Laboratory of Health Science, Postgraduate Program in Health Sciences, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais, Brazil; Department of Dentistry, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais, Brazil.
authors.forename: André Luiz Sena
authors.initials: AL
authors.lastname: Guimarães
authors.affiliation: Laboratory of Health Science, Postgraduate Program in Health Sciences, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais, Brazil; Department of Dentistry, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais, Brazil.
authors.forename: Alfredo Maurício Batista
authors.initials: AM
authors.lastname: de Paula
authors.affiliation: Institute of Biological Sciences, Department of Pharmacology, Universidade Federal de Minas Gerais, Minas Gerais, Brazil.
authors.forename: Maria José
authors.initials: MJ
authors.lastname: Campagnole-Santos
authors.affiliation: Institute of Biological Sciences, Department of Pharmacology, Universidade Federal de Minas Gerais, Minas Gerais, Brazil.
authors.forename: Robson Augusto
authors.initials: RA
authors.lastname: dos Santos
authors.affiliation: Laboratory of Health Science, Postgraduate Program in Health Sciences, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais, Brazil; Institute of Biological Sciences, Department of Pharmacology, Universidade Federal de Minas Gerais, Minas Gerais, Brazil; Department of Physiology and Biophysics, Universidade Federal de Minas Gerais, Minas Gerais, Brazil. Electronic address: sergiosousas@hotmail.com.
authors.forename: Sérgio Henrique Sousa
authors.initials: SH
authors.lastname: Santos
mesh_terms: D000284:Administration, Oral; D000803:Angiotensin I / Q000008:administration & dosage*; D000818:Animals; D000963:Antimetabolites / Q000008:administration & dosage; D002478:Cells, Cultured; D059305:Diet, High-Fat / Q000009:adverse effects*; D004353:Drug Evaluation, Preclinical; D015870:Gene Expression; D018149:Glucose Intolerance / Q000188:drug therapy / Q000209:etiology / Q000378:metabolism; D006946:Hyperinsulinism / Q000188:drug therapy / Q000209:etiology / Q000378:metabolism; D007333:Insulin Resistance; D050152:Intra-Abdominal Fat / Q000378:metabolism* / Q000473:pathology; D008066:Lipolysis; D008297:Male; D051379:Mice; D009765:Obesity / Q000188:drug therapy / Q000209:etiology / Q000378:metabolism*; D010446:Peptide Fragments / Q000008:administration & dosage*; D061251:Primary Cell Culture; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism*; D012084:Renin-Angiotensin System / Q000187:drug effects; D052243:Resistin / Q000097:blood; D000077185:Resveratrol; D037761:Sirtuins / Q000235:genetics / Q000378:metabolism*; D013267:Stilbenes / Q000008:administration & dosage
chemical_list: D000963:Antimetabolites; D010446:Peptide Fragments; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D043562:Receptors, G-Protein-Coupled; D052243:Resistin; C499623:Retn protein, mouse; D013267:Stilbenes; D000803:Angiotensin I; D037761:Sirtuins; C118790:angiotensin I (1-7); D000077185:Resveratrol
keywords: Ang-(1–7); Metabolic syndrome; Obesity; Rennin-angiotensin system; Resveratrol