title: Cytotoxic efficacy of a novel dinuclear platinum(II) complex used with anti-MUC1 in human breast cancer cells.
abstract: Mucin 1 (MUC1) is overexpressed in various cancer cells especially in breast cancer cells. There are known research works on the use of anti-MUC1 antibody with docetaxel in ovarian cancer, but there are no data about combined therapy platinum compounds with anti-MUC1 in breast cancer. The aim of the study was to evaluate the antiproliferative properties of a new dinuclear platinum(II) complex (Pt12) used with anti-MUC1 in human breast cancer cells. The dinuclear platinum(II) complex (Pt12) has been synthesized, and its cytotoxicity with anti-MUC1 has been tested in both MCF-7 and MDA-MB-231 breast cancer cells. In this study, the effects of Pt12 with anti-MUC1 on collagen and DNA biosynthesis in human breast cancer cells were compared to those evoked by cisplatin and cisplatin with anti-MUC1. The mechanism of action of Pt12 with anti-MUC1 was studied employing flow cytometry assessment of annexin V binding assay. It was found that Pt12 with anti-MUC1 was more active inhibitor of DNA and collagen synthesis as well more cytotoxic agent than Pt12 alone and cisplatin with anti-MUC1. Cytotoxicity of Pt12 with anti-MUC1 against breast cancer cells is due to apoptotic cell death as well as necrotic cell death. These results indicate that the use of Pt12 with anti-MUC1 may constitute a novel strategy in the chemotherapy of breast cancer tumors.
authors.affiliation: Department of Biotechnology, Medical University of Bialystok, Kilińskiego 1, 15-222, Białystok, Poland, agnieszka.gornowicz@umb.edu.pl.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Gornowicz
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Kałuża
authors.forename: Anna
authors.initials: A
authors.lastname: Bielawska
authors.forename: Halina
authors.initials: H
authors.lastname: Gabryel-Porowska
authors.forename: Robert
authors.initials: R
authors.lastname: Czarnomysy
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Bielawski
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D005260:Female; D006801:Humans; D018396:Mucin-1 / Q000276:immunology*; D009944:Organoplatinum Compounds / Q000494:pharmacology*
chemical_list: D000911:Antibodies, Monoclonal; D000970:Antineoplastic Agents; C060192:MUC1 protein, human; D018396:Mucin-1; D009944:Organoplatinum Compounds