title: Contribution of human oocyte architecture to success of in vitro maturation technology.
abstract: The use of ovarian stimulation for infertility treatment is associated with side effects of ovarian hyperstimulation syndrome (OHSS) and potential cancer risk. This is also true in high risk women such as those polycystic with ovary (PCO) and polycystic ovarian syndrome (PCOS). In vitro maturation (IVM) of oocytes was primarily developed to make IVF safe for women with PCO and at high risk of OHSS. The application of IVM of oocytes to assist clinical infertility treatment remains poor because of the reduced developmental competence of oocytes after IVM, despite several decades of research. Reduced meiotic maturation and fertilization rates, as well as low blastocyst production reveal short-term developmental insufficiency of oocytes when compared with in vivo-matured counterparts. In this review, the structural role of human oocytes, revealed by different technical approaches, to the success of IVM technology is highlighted.
authors.affiliation: Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
authors.forename: Mohammad Ali
authors.initials: MA
authors.lastname: Khalili
authors.affiliation: Department of Anatomy, Histology, Forensic Medicine and Orthopaedics, La Sapienza University, Rome, Italy.
authors.forename: Stefania
authors.initials: S
authors.lastname: A Nottola
authors.affiliation: Department of Anatomy, Yazd Branch, Islamic Azad University, Yazd, Iran.
authors.forename: Abbas
authors.initials: A
authors.lastname: Shahedi
authors.affiliation: Department of Life, Health and Environmental Sciences, University of L'Aquila, L'Aquila, Italy.
authors.forename: Guido
authors.initials: G
authors.lastname: Macchiarelli
keywords: Cell Morphology; Human; In Vitro Oocyte Maturation Technique; Oocyte; Ultrastructure