title: Bioactive metabolites from the mycelia of the basidiomycete Hericium erinaceum.
abstract: Seven known compounds, three diketopiperazine alkaloids, 12β-hydroxyverruculogen TR-2 (1), fumitremorgin C (2) and methylthiogliotoxin (5), two hetero-spirocyclic γ-lactam alkaloids, pseurotin A (3) and FD-838 (4), and cerevisterol (6) and herierin IV (7), were isolated from the mycelia of the basidiomycete Hericium erinaceum and identified by spectroscopic analyses. The antioxidant and antifungal activities of compounds 1-6 were evaluated. The results indicated that compounds 1, 3 and 6 exhibited potential antioxidant activity against DPPH (2, 2-diphenyl-1-picrylhydrazyl) radical with their IC50 data of ca. 12 μM, compared with positive control tertiary butylhydroquinone. In addition, compound 4 significantly inhibited the growth of two plant fungal pathogens Botrytis cinerea and Glomerella cingulata with an minimum inhibitory concentration of 6.25 μM for each, similar to that of the positive fungicide, carbendazim. Compounds 1-5 were isolated from the genus Hericium for the first time.
authors.affiliation: a Department of Applied Chemistry, College of Science, Shaanxi Engineering Center of Bioresource Chemistry & Sustainable Utilization, Northwest A&F University , Yangling 712100 , Shaanxi , P.R. China.
authors.forename: Qiang-Qiang
authors.initials: QQ
authors.lastname: Lu
authors.forename: Jun-Mian
authors.initials: JM
authors.lastname: Tian
authors.forename: Jing
authors.initials: J
authors.lastname: Wei
authors.forename: Jin-Ming
authors.initials: JM
authors.lastname: Gao
mesh_terms: D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D001487:Basidiomycota / Q000737:chemistry*; D001713:Biphenyl Compounds / Q000494:pharmacology; D002681:China; D054659:Diketopiperazines / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D005663:Furans / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D007093:Imidazoles / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D020128:Inhibitory Concentration 50; D015394:Molecular Structure; D025282:Mycelium / Q000737:chemistry; D019906:Nuclear Magnetic Resonance, Biomolecular; D010851:Picrates / Q000494:pharmacology
chemical_list: D000935:Antifungal Agents; D000975:Antioxidants; D001713:Biphenyl Compounds; D054659:Diketopiperazines; C529961:FD 838; D005663:Furans; D007093:Imidazoles; D010851:Picrates; C004931:1,1-diphenyl-2-picrylhydrazyl
keywords: Hericium erinaceum; antifungal activity; antioxidant activity; diketopiperazine alkaloids; mushroom