title: Tanay virus, a new species of virus isolated from mosquitoes in the Philippines.
abstract: In 2005, we isolated a new species of virus from mosquitoes in the Philippines. The virion was elliptical in shape and had a short single projection. The virus was named Tanay virus (TANAV) after the locality in which it was found. TANAV genomic RNA was a 9562 nt+poly-A positive strand, and polycistronic. The longest ORF contained putative RNA-dependent RNA polymerase (RdRP); however, conserved short motifs in the RdRP were permuted. TANAV was phylogenetically close to Negevirus, a recently proposed taxon of viruses isolated from haemophagic insects, and to some plant viruses, such as citrus leprosis virus C, hibiscus green spot virus and blueberry necrotic ring blotch virus. In this paper, we describe TANAV and the permuted structure of its RdRP, and discuss its phylogeny together with those of plant viruses and negevirus.
authors.affiliation: Department of Virology, Institute of Tropical Medicine, Nagasaki University, Sakamoto-machi 1-12-4, Nagasaki City 852-8523, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Nabeshima
authors.affiliation: Department of Virology, Institute of Tropical Medicine, Nagasaki University, Sakamoto-machi 1-12-4, Nagasaki City 852-8523, Japan.
authors.forename: Shingo
authors.initials: S
authors.lastname: Inoue
authors.affiliation: Department of Virology, Institute of Tropical Medicine, Nagasaki University, Sakamoto-machi 1-12-4, Nagasaki City 852-8523, Japan.
authors.forename: Kenta
authors.initials: K
authors.lastname: Okamoto
authors.affiliation: Department of Virology, Institute of Tropical Medicine, Nagasaki University, Sakamoto-machi 1-12-4, Nagasaki City 852-8523, Japan.
authors.forename: Guillermo
authors.initials: G
authors.lastname: Posadas-Herrera
authors.affiliation: Department of Virology, Institute of Tropical Medicine, Nagasaki University, Sakamoto-machi 1-12-4, Nagasaki City 852-8523, Japan.
authors.forename: Fuxun
authors.initials: F
authors.lastname: Yu
authors.affiliation: Department of Virology, Institute of Tropical Medicine, Nagasaki University, Sakamoto-machi 1-12-4, Nagasaki City 852-8523, Japan.
authors.forename: Leo
authors.initials: L
authors.lastname: Uchida
authors.affiliation: Central Laboratory, Institute of Tropical Medicine, Nagasaki University, Nagasaki University, Sakamoto-machi 1-12-4, Nagasaki City 852-8523, Japan.
authors.forename: Akitoyo
authors.initials: A
authors.lastname: Ichinose
authors.affiliation: Central Laboratory, Institute of Tropical Medicine, Nagasaki University, Nagasaki University, Sakamoto-machi 1-12-4, Nagasaki City 852-8523, Japan.
authors.forename: Miako
authors.initials: M
authors.lastname: Sakaguchi
authors.affiliation: Department of Vector Ecology and Environment, Institute of Tropical Medicine, Nagasaki University, Sakamoto-machi 1-12-4, Nagasaki City 852-8523, Japan.
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Sunahara
authors.affiliation: Research and Biotechnology, St. Luke's Medical Center, 279 E. Rodriguez Sr. Blvd, Quezon City 1102, Philippines.|Department of Virology, Institute of Tropical Medicine, Nagasaki University, Sakamoto-machi 1-12-4, Nagasaki City 852-8523, Japan.
authors.forename: Corazon C
authors.initials: CC
authors.lastname: Buerano
authors.affiliation: Research and Biotechnology, St. Luke's Medical Center, 279 E. Rodriguez Sr. Blvd, Quezon City 1102, Philippines.
authors.forename: Florencio P
authors.initials: FP
authors.lastname: Tadena
authors.affiliation: Research and Biotechnology, St. Luke's Medical Center, 279 E. Rodriguez Sr. Blvd, Quezon City 1102, Philippines.
authors.forename: Ildefonso B
authors.initials: IB
authors.lastname: Orbita
authors.affiliation: Research and Biotechnology, St. Luke's Medical Center, 279 E. Rodriguez Sr. Blvd, Quezon City 1102, Philippines.
authors.forename: Filipinas F
authors.initials: FF
authors.lastname: Natividad
authors.affiliation: Department of Virology, Institute of Tropical Medicine, Nagasaki University, Sakamoto-machi 1-12-4, Nagasaki City 852-8523, Japan.
authors.forename: Kouichi
authors.initials: K
authors.lastname: Morita
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003465:Culex / Q000821:virology; D009033:Culicidae / Q000821:virology*; D016679:Genome, Viral; D007304:Insect Viruses / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010679:Philippines; D010802:Phylogeny; D010942:Plant Viruses / Q000235:genetics; D012328:RNA Viruses / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000235:genetics; D014771:Virion / Q000648:ultrastructure; D014713:Viruses, Unclassified / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D014764:Viral Proteins; D012324:RNA-Dependent RNA Polymerase