title: Complete genome sequence of the Pectobacterium carotovorum subsp. carotovorum virulent bacteriophage PM1.
abstract: PM1, a novel virulent bacteriophage that infects Pectobacterium carotovorum subsp. carotovorum, was isolated. Its morphological features were examined by electron microscopy, which indicated that this phage belongs to the family Myoviridae. It has a 55,098-bp genome, including a 2,665-bp terminal repeat. A total of 63 open reading frames (ORFs) were predicted, but only 20 ORFs possessed homology with functional proteins. There is one tRNA coding region, and the GC-content of the genome is 44.9 %. Most ORFs in bacteriophage PM1 showed high homology to enterobacteria phage ΦEcoM-GJ1 and Erwinia phage νB EamM-Y2. Like these bacteriophages, PM1 encodes an RNA polymerase, which is a hallmark of T7-like phages. There is no integrase or repressor, suggesting that PM1 is a virulent bacteriophage.
authors.affiliation: Microbial Safety Division, National Academy of Agricultural Science, Rural Development Administration, Suwon, 441-707, Korea.
authors.forename: Jeong-A
authors.initials: JA
authors.lastname: Lim
authors.forename: Hakdong
authors.initials: H
authors.lastname: Shin
authors.forename: Dong Hwan
authors.initials: DH
authors.lastname: Lee
authors.forename: Sang-Wook
authors.initials: SW
authors.lastname: Han
authors.forename: Ju-Hoon
authors.initials: JH
authors.lastname: Lee
authors.forename: Sangryeol
authors.initials: S
authors.lastname: Ryu
authors.forename: Sunggi
authors.initials: S
authors.lastname: Heu
mesh_terms: D001435:Bacteriophages / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D012321:DNA-Directed RNA Polymerases / Q000235:genetics / Q000378:metabolism; D016679:Genome, Viral; D008969:Molecular Sequence Data; D017900:Myoviridae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016366:Open Reading Frames; D016973:Pectobacterium carotovorum / Q000821:virology*; D010802:Phylogeny; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D014764:Viral Proteins; D012321:DNA-Directed RNA Polymerases