title: High expression of MICA in human kidney cancer tissue and renal cell carcinoma lines.
abstract: The overall incidence and mortality of renal cell carcinoma (RCC), the most common kidney cancer, are steadily increasing for reasons that are not fully explained. Our aim was to explore the expression of membrane MHC class I chain-related gene A (mMICA) in human RCC cell lines and tissue specimens, and to determine expression of soluble MICA (sMICA) in serum of patients with renal cell carcinoma, we used flow cytometry (FCM) and immunohistochemistry as well as an enzyme linked immunosorbent assay (ELISA) . The results showed that percentage of mMICA expression was significantly increased in human kidney cancer tissues and RCC cell lines (786-O and Ketr-3) than that in healthy adults and human embryonic kidney 293 (HEK293) cell line individuality (P<0.05). sMICA content in healthy adults was negative, but in renal cancer patients was significantly elevated (P<0.05). Our research showed that high expression of MICA in human kidney cancer, this results show that MICA might serve as potential tumor-associated antigen (TAA) in RCC.
authors.affiliation: Department of Evidence-Based Medicine, The Second Hospital, Shandong University, Jinan, China E-mail : jingjiezhao@aliyun.com.
authors.forename: Hong-Ying
authors.initials: HY
authors.lastname: Jia
authors.forename: Jun-Li
authors.initials: JL
authors.lastname: Liu
authors.forename: Cheng-Jun
authors.initials: CJ
authors.lastname: Zhou
authors.forename: Feng
authors.initials: F
authors.lastname: Kong
authors.forename: Ming-Zhen
authors.initials: MZ
authors.lastname: Yuan
authors.forename: Wen-Dong
authors.initials: WD
authors.lastname: Sun
authors.forename: Jue
authors.initials: J
authors.lastname: Wang
authors.forename: Ling
authors.initials: L
authors.lastname: Liu
authors.forename: Jing-Jie
authors.initials: JJ
authors.lastname: Zhao
authors.forename: Yun
authors.initials: Y
authors.lastname: Luan
mesh_terms: D000951:Antigens, Neoplasm; D002292:Carcinoma, Renal Cell / Q000097:blood* / Q000453:epidemiology / Q000401:mortality; D045744:Cell Line, Tumor; D004797:Enzyme-Linked Immunosorbent Assay; D057809:HEK293 Cells; D015395:Histocompatibility Antigens Class I / Q000096:biosynthesis* / Q000097:blood* / Q000235:genetics; D006801:Humans; D007680:Kidney Neoplasms / Q000097:blood* / Q000453:epidemiology / Q000401:mortality; D008565:Membrane Proteins / Q000096:biosynthesis / Q000097:blood*; D012333:RNA, Messenger / Q000096:biosynthesis
chemical_list: D000951:Antigens, Neoplasm; D015395:Histocompatibility Antigens Class I; C099187:MHC class I-related chain A; D008565:Membrane Proteins; D012333:RNA, Messenger