title: Expression of PDZ-binding kinase/T-LAK cell-originated protein kinase (PBK/TOPK) in human urinary bladder transitional cell carcinoma.
abstract: The objective of this study was to evaluate the expression pattern of PDZ-binding kinase/T-LAK cell-originated protein kinase (PBK/TOPK) and its clinical significance in human bladder cancer (BC). We detected PBK/TOPK mRNA overexpression in BC and human normal testis tissues using RT-PCR. Using qRT-PCR revealed a higher expression of PBK/TOPK in BC tissues than their adjacent noncancerous tissues (ANCTs) (p<0.0001). Cytoplasmic expression of PBK/TOPK protein was found to be positive in 64.6% (42 of 65) BC patients. Expression of PBK/TOPK protein was found to be significantly higher in muscle-invasive bladder cancer (MIBC) than in non-muscle-invasive bladder cancer (NMIBC) (86.1% vs. 37.9%, p<0.001). The immunohistochemical (IHC) expression of PBK/TOPK was found to be significantly (p<0.001) associated with the stage of disease. Study findings suggest that the PBK/TOPK mRNA/protein expression is specific to human BC and might be used as a novel target for development of cancer immunotherapy and diagnostic biomarker.
authors.affiliation: Department of Radiotherapy, King George's Medical University, Lucknow 226003, Uttar Pradesh, India.
authors.forename: P K
authors.initials: PK
authors.lastname: Singh
authors.affiliation: Department of Radiotherapy, King George's Medical University, Lucknow 226003, Uttar Pradesh, India.
authors.forename: Anupam K
authors.initials: AK
authors.lastname: Srivastava
authors.affiliation: Department of Urology, King George's Medical University, Lucknow 226003, Uttar Pradesh, India.
authors.forename: D
authors.initials: D
authors.lastname: Dalela
authors.affiliation: Genotoxicity Laboratory, Division of Toxicology, Central Drug Research Institute (CSIR), Lucknow 226001, Uttar Pradesh , India.
authors.forename: S K
authors.initials: SK
authors.lastname: Rath
authors.affiliation: Department of Pathology, King George's Medical University, Lucknow 226003, Uttar Pradesh, India.
authors.forename: M M
authors.initials: MM
authors.lastname: Goel
authors.affiliation: Department of Radiation Oncology, Dr. Ram Manohar Lohia Institute of Medical Sciences, Lucknow 226010, Uttar Pradesh, India. Electronic address: mlbbhatt@yahoo.co.in.
authors.forename: M L B
authors.initials: ML
authors.lastname: Bhatt
mesh_terms: D014408:Biomarkers, Tumor / Q000096:biosynthesis / Q000235:genetics; D002295:Carcinoma, Transitional Cell / Q000235:genetics* / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D020929:Mitogen-Activated Protein Kinase Kinases / Q000096:biosynthesis* / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis; D013737:Testis / Q000378:metabolism; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; D012333:RNA, Messenger; D020929:Mitogen-Activated Protein Kinase Kinases; C409022:PDZ-binding kinase
keywords: BCG; Cancer-testis antigen; Immunotherapy; PBK/TOPK; TCC; Vaccine