title: Augmentation of intramembranous bone in rabbit calvaria using an occlusive barrier in combination with demineralized bone matrix (DBM): a pilot study.
abstract: The aim of this study was to histologically evaluate the performance of demineralized bone matrix (DBM) when compared with a blood clot in addition to an occlusive barrier in the bone regeneration process for bone defects in a rabbit model. Prefabricated metallic capsules with 4.5 mm and 3.5 mm dimensions were placed in five adult rabbit skulls. At the right side, the capsule was filled with DBM, and the clot was located on the left side. The barriers were supplied with a 0.5 mm horizontal peripheral flap and a vertical edge, fitting tightly into a circular slit prepared by a trephine in the skull. After a healing period of three months, the animals were sacrificed, and the samples were prepared for histological and histomorphometric analyses after capsule removal. Trabecular and medullar bone percentages were calculated from the different areas of the newly formed bone inside the metallic barriers, and non-parametric statistical analysis was used to describe the findings. The results showed a complete filling of newly formed bone inside the capsules of both groups. Less mature bone tissue was observed in the upper third of all samples, and a higher trabecular area was observed in the samples with DBM. The use of barriers resulted in the augmentation of newly formed bone in a three-month period. However, a higher trabecular area was observed in the barriers filled with DBM.
authors.affiliation: Department of Maxillofacial Surgery, University of Göttingen, Germany; Departamento Integral del Adulto, Facultad de Odontología, Universidad de La Frontera, Chile. Electronic address: victor.beltran@ufrontera.cl.
authors.forename: Víctor
authors.initials: V
authors.lastname: Beltrán
authors.affiliation: Department of Maxillofacial Surgery, University of Göttingen, Germany.
authors.forename: Wilfried
authors.initials: W
authors.lastname: Engelke
authors.affiliation: Facultad de Medicina, Universidad de La Frontera, Chile.
authors.forename: Ruth
authors.initials: R
authors.lastname: Prieto
authors.affiliation: Unidad de Anatomía Humana, Departamento Biomédico, Universidad de Antofagasta, Chile.
authors.forename: Iván
authors.initials: I
authors.lastname: Valdivia-Gandur
authors.affiliation: Departamento de Matemáticas y Estadística, Facultad de Ingeniería, Universidad de La Frontera, Chile.
authors.forename: Pablo
authors.initials: P
authors.lastname: Navarro
authors.affiliation: Unidad de Anatomía y Embriología Humana, Health University of Barcelona campus-Bellvitge, Universidad de Barcelona, Spain.
authors.forename: María Cristina
authors.initials: MC
authors.lastname: Manzanares
authors.affiliation: Departamento Integral del Adulto, Facultad de Odontología, Universidad de La Frontera, Chile; Ribeirão Preto Dental School, University of São Paulo, Ribeirão Preto, Brazil.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Borie
authors.affiliation: Departamento Integral del Adulto, Facultad de Odontología, Universidad de La Frontera, Chile.
authors.forename: Ramón
authors.initials: R
authors.lastname: Fuentes
mesh_terms: D000818:Animals; D001777:Blood Coagulation; D001861:Bone Regeneration / Q000187:drug effects / Q000502:physiology*; D018786:Bone Substitutes / Q000737:chemistry* / Q000494:pharmacology; D008422:Materials Testing; D010865:Pilot Projects; D019919:Prosthesis Implantation; D011817:Rabbits; D012886:Skull / Q000293:injuries / Q000502:physiology / Q000601:surgery*; D023822:Tissue Engineering
chemical_list: D018786:Bone Substitutes
keywords: Barriers; Biomaterials; Bone; Bone regeneration; DBM