title: Overexpression of EphB4, EphrinB2, and epidermal growth factor receptor in papillary thyroid carcinoma: A pilot study.
abstract: BACKGROUND
  The purpose of this study was to examine the differential expression of EphB4, EphrinB2, and epidermal growth factor receptor (EGFR) genes in papillary thyroid carcinoma (PTC) and evaluate their association with lymph node metastasis.
  
  
  METHODS
  EphB4, EphrinB2, and EGFR expression in 21 matched tumors and surrounding normal thyroid tissues were evaluated by complementary DNA (cDNA) microarray, Western blot, and immunohistochemistry (IHC).
  
  
  RESULTS
  We noted a statistically significant overexpression of EphB4, EphrinB2, and EGFR in tumor versus normal tissue based on cDNA microarray, Western blot, and IHC analysis. EphB4 and EphrinB2 overexpression were significantly associated with the presence of lymph node disease.
  
  
  CONCLUSIONS
  Overexpression of EphB4, EphrinB2, and EGFR are associated with PTC, whereas EphB4 and EphrinB2 overexpression are associated with lymph node metastases. These genes may be potential biomarkers for identification of subclinical lymph node involvement in PTC and potential small-molecule targets for pharmacotherapy research.
authors.affiliation: Department of Otolaryngology - Head and Neck Surgery, Keck School of Medicine of University of Southern California, Los Angeles, California.
authors.forename: Giriraj K
authors.initials: GK
authors.lastname: Sharma
authors.affiliation: Department of Otolaryngology - Head and Neck Surgery, Keck School of Medicine of University of Southern California, Los Angeles, California.
authors.forename: Vaninder K
authors.initials: VK
authors.lastname: Dhillon
authors.affiliation: Department of Otolaryngology - Head and Neck Surgery, Keck School of Medicine of University of Southern California, Los Angeles, California.
authors.forename: Rizwan
authors.initials: R
authors.lastname: Masood
authors.affiliation: Department of Otolaryngology - Head and Neck Surgery, Keck School of Medicine of University of Southern California, Los Angeles, California.
authors.forename: Dennis R
authors.initials: DR
authors.lastname: Maceri
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015153:Blotting, Western; D002277:Carcinoma / Q000378:metabolism*; D002291:Carcinoma, Papillary; D036388:Ephrin-B2 / Q000378:metabolism*; D066246:ErbB Receptors / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D010865:Pilot Projects; D036224:Receptor, EphB4 / Q000378:metabolism*; D000077273:Thyroid Cancer, Papillary; D013961:Thyroid Gland / Q000378:metabolism* / Q000473:pathology; D013964:Thyroid Neoplasms / Q000378:metabolism*; D055815:Young Adult
chemical_list: D036388:Ephrin-B2; C512478:EGFR protein, human; D066246:ErbB Receptors; D036224:Receptor, EphB4
keywords: Ephb4; Ephrinb2; epidermal growth factor receptor (EGFR); gene; papillary; thyroid