title: Preliminary X-ray crystallographic analysis of glutathione transferase zeta 1 (GSTZ1a-1a).
abstract: Glutathione transferase zeta 1 (GSTZ1-1) is a homodimeric enzyme found in the cytosol and mitochondrial matrix of the liver and other tissues. It catalyzes the glutathione-dependent isomerization of maleylacetoacetate to fumarylacetoacetate in the tyrosine catabolic pathway and can metabolize small halogenated carboxylic acids. GSTZ1a-1a crystals diffracted to a resolution of 3.1 Å and belonged to space group P1, with unit-cell parameters a = 42.0, b = 49.6, c = 54.6 Å, α = 82.9, β = 69.9, γ = 73.4°, with a calculated Matthews coefficient of 2.1 Å(3) Da(-1) assuming a dimer in the crystallographic asymmetric unit. Refinement of the structure is currently in progress.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Florida, PO Box 100245, Gainesville, FL 32610, USA.
authors.forename: Christopher D
authors.initials: CD
authors.lastname: Boone
authors.affiliation: Department of Medicinal Chemistry, University of Florida, PO Box 100485, Gainesville, FL 32610, USA.
authors.forename: Guo
authors.initials: G
authors.lastname: Zhong
authors.affiliation: Department of Medicinal Chemistry, University of Florida, PO Box 100485, Gainesville, FL 32610, USA.
authors.forename: Marci
authors.initials: M
authors.lastname: Smeltz
authors.affiliation: Department of Medicinal Chemistry, University of Florida, PO Box 100485, Gainesville, FL 32610, USA.
authors.forename: Margaret O
authors.initials: MO
authors.lastname: James
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Florida, PO Box 100245, Gainesville, FL 32610, USA.
authors.forename: Robert
authors.initials: R
authors.lastname: McKenna
mesh_terms: D018360:Crystallography, X-Ray / Q000379:methods*; D005982:Glutathione Transferase / Q000737:chemistry*; D006801:Humans; D007527:Isoenzymes / Q000737:chemistry*; D011487:Protein Conformation
chemical_list: D007527:Isoenzymes; C407705:GSTZ1 protein, human; D005982:Glutathione Transferase
keywords: GSTZ1a-1a; glutathione transferase