title: Allele-mining of rice blast resistance genes at AC134922 locus.
abstract: The AC134922 locus is one of the most rapidly evolving nucleotide binding site-leucine-rich repeat (NBS-LRR) gene family in rice genome. Six rice blast resistance (R) genes have been cloned from this locus and other two resistance candidate genes, Pi34 and Pi47, are also mapped to this complex locus. Therefore, it seems that more functional R genes could be identified from this locus. In this study, we cloned 22 genes from 12 cultivars based on allele-mining strategy at this locus and identified 6 rice blast R genes with 4 of them recognizing more than one isolates. Our result suggests that gene stacking might be the evolutionary strategy for complex gene locus to interact with rapidly evolving pathogens, which might provide a potential way for the cloning of durable resistance genes. Moreover, the mosaic structure and ambiguous ortholog/paralog relationships of these homologous genes, caused by frequent recombination and gene conversion, indicate that multiple alleles of this complex locus may serve as a reservoir for the evolutionary novelty of these R genes.
authors.affiliation: State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, 210093 Nanjing, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, 210093 Nanjing, China.
authors.forename: Changjiang
authors.initials: C
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, 210093 Nanjing, China.
authors.forename: Ju
authors.initials: J
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, 210093 Nanjing, China.
authors.forename: Sihai
authors.initials: S
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, 210093 Nanjing, China. Electronic address: dtian@nju.edu.cn.
authors.forename: Dacheng
authors.initials: D
authors.lastname: Tian
authors.affiliation: State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, 210093 Nanjing, China. Electronic address: xiaohuizhang@nju.edu.cn.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D017343:Genes, Plant; D056426:Genetic Loci; D008969:Molecular Sequence Data; D012275:Oryza / Q000737:chemistry / Q000235:genetics*; D010802:Phylogeny; D010935:Plant Diseases / Q000235:genetics*; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*
chemical_list: D010940:Plant Proteins
keywords: AC134922; Allele-mining; Gene conversion; Resistance