title: Use of mesenchymal stem cells for cutaneous repair and skin substitute elaboration.
abstract: Human mesenchymal stem cells (MSCs) are a heterogeneous population of fibroblast-like cells, which are present in different locations, including bone marrow, adipose tissue, extra-foetal tissues, gingiva and dermis. MSCs, which present multipotency capacities, important expansive potential and immunotolerance properties, remain an attractive tool for tissue repair and regenerative medicine. Currently, several studies and clinical trials highlight the use of MSCs in cutaneous repair underlining that their effects are essentially due to the numerous factors that they release. MSCs are also used in skin substitute development. In this study, we will first discuss the different sources of MSCs actually available. We will then present results showing that bone marrow-derived MSCs prepared according to Good Manufacturing Practices and included in a dermal equivalent are able to promote appropriate epidermis growth and differentiation. These data demonstrate that bone marrow-derived MSCs represent a satisfactory alternative to dermal fibroblasts in order to develop skin substitute. In addition, MSCs could provide a useful alternative to sustain epidermis development and to promote wound healing.
authors.affiliation: Département de physiologie et EA6309, FR 3503, faculté de médecine et de pharmacie, université de Limoges, 2, rue du Docteur-Marcland, 87025 Limoges cedex, France.
authors.forename: B
authors.initials: B
authors.lastname: Laverdet
authors.affiliation: Département de physiologie et EA6309, FR 3503, faculté de médecine et de pharmacie, université de Limoges, 2, rue du Docteur-Marcland, 87025 Limoges cedex, France.
authors.forename: L
authors.initials: L
authors.lastname: Micallef
authors.affiliation: Inserm U989, faculté de médecine, université Paris-Descartes, 15, rue de l'École-de-Médecine, 75006 Paris, France.
authors.forename: C
authors.initials: C
authors.lastname: Lebreton
authors.affiliation: Hôpital de la Mère et de l'Enfant, CHU Dupuytren, 8, avenue Dominique-Larrey, 87042 Limoges cedex, France.
authors.forename: J
authors.initials: J
authors.lastname: Mollard
authors.affiliation: Département recherche et thérapies cellulaires, centre de transfusion sanguine des Armées, hôpital d'instruction des Armées-Percy, 101, avenue Henri-Barbusse, 92141 Clamart, France.
authors.forename: J-J
authors.initials: JJ
authors.lastname: Lataillade
authors.affiliation: Inserm U970, Équipe réparation artérielle, PARCC-HEGP, université Paris-Descartes, 56, rue Leblanc, 75015 Paris, France.
authors.forename: B
authors.initials: B
authors.lastname: Coulomb
authors.affiliation: Département de physiologie et EA6309, FR 3503, faculté de médecine et de pharmacie, université de Limoges, 2, rue du Docteur-Marcland, 87025 Limoges cedex, France. Electronic address: alexis.desmouliere@unilim.fr.
authors.forename: A
authors.initials: A
authors.lastname: Desmoulière
mesh_terms: D002454:Cell Differentiation; D002478:Cells, Cultured; D020405:Dermis / Q000166:cytology; D000078404:Epidermal Cells; D005347:Fibroblasts / Q000637:transplantation; D006801:Humans; D020782:Matrix Metalloproteinases / Q000378:metabolism; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000201:enzymology; D009928:Organ Specificity; D012867:Skin / Q000293:injuries; D017768:Skin, Artificial; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D058106:Wound Closure Techniques; D014945:Wound Healing
chemical_list: D020782:Matrix Metalloproteinases
keywords: (Myo)fibroblastes; (Myo)fibroblasts; Cellules souches mésenchymateuses; Mesenchymal stem cells; Peau reconstruite; Reconstituted skin; Réparation tissulaire; Tissue repair