title: Differentiation of Klebsiella pneumoniae carbapenemase (KPC) variants by pyrosequencing.
abstract: A fast and reliable protocol using the pyrosequencing technique was developed to identify 11 different types of the KPC enzyme. A total of 65 blaKPC positive bacterial isolates were tested and characterized. In the end, the pyrosequencing proved to be a powerful tool for epidemiological studies of KPC producer isolates.
authors.affiliation: Esoteric Testing Laboratory, Pathology Department, Tampa General Hospital, Tampa, United States; Laboratório Especial de Microbiologia Clínica, Universidade Federal de São Paulo, São Paulo, Brazil.
authors.forename: Jussimara
authors.initials: J
authors.lastname: Monteiro
authors.affiliation: Esoteric Testing Laboratory, Pathology Department, Tampa General Hospital, Tampa, United States.
authors.forename: Raymond H
authors.initials: RH
authors.lastname: Widen
authors.affiliation: Laboratório Especial de Microbiologia Clínica, Universidade Federal de São Paulo, São Paulo, Brazil.
authors.forename: Antonio C C
authors.initials: AC
authors.lastname: Pignatari
authors.affiliation: Esoteric Testing Laboratory, Pathology Department, Tampa General Hospital, Tampa, United States.
authors.forename: Carly
authors.initials: C
authors.lastname: Kubasek
authors.affiliation: Esoteric Testing Laboratory, Pathology Department, Tampa General Hospital, Tampa, United States. Electronic address: ssilbert@tgh.org.
authors.forename: Suzane
authors.initials: S
authors.lastname: Silbert
mesh_terms: D001426:Bacterial Proteins / Q000145:classification* / Q000235:genetics*; D014644:Genetic Variation; D006801:Humans; D007710:Klebsiella Infections / Q000453:epidemiology / Q000382:microbiology; D007711:Klebsiella pneumoniae / Q000145:classification / Q000201:enzymology* / Q000235:genetics; D017720:Molecular Epidemiology / Q000379:methods; D017422:Sequence Analysis, DNA / Q000379:methods*; D001618:beta-Lactamases / Q000145:classification* / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D001618:beta-Lactamases; C063912:carbapenemase
keywords: KPC; Klebsiella pneumoniae carbapenemase; Pyrosequencing; Real-time PCR