title: Effect of caffeine on motility and vitality of sperm and in vitro fertilization of outbreed mouse in T6 and M16 media.
abstract: BACKGROUND
  Caffeine increases the CAMP production that stimulates spermatozoa movement. Caffeine is also used for induction of in vitro acrosome reaction in mammalian spermatozoa, an important step in achieving fertilization.
  
  
  OBJECTIVE
  The aim of this study was to assess the effect of caffeine on sperm's motility, vitality and laboratory fertilization rates in mouse in two T6 and M16 media.
  
  
  METHODS
  Epididymal mouse sperms were collected and treated by caffine in T6 and M16 media and their motility and vitality rates were evaluated. The pretreated sperms were added to oocytes in T6 and M16 media with and without caffeine and fertilization rates were recorded after 24 hours incubation.
  
  
  RESULTS
  Sperm's motility (81.7±1.67%) and vitality (88.7±1.33%) rates and percentage of fertilized oocytes (67.52±8.16%) in T6 medium plus caffeine compare to control group have increased and shown significant differences at p≤0.01. While the percentages of these parameters in M16 medium supplemented with caffeine were 68.3±6.01%, 78±6.11%, and 42.6±12.96 respectively and in comparison to control group (M16 without caffeine) have not shown significant differences.
  
  
  CONCLUSIONS
  Addition of caffeine to T6 medium promotes the sperm's motility and vitality and enhances fertilization and early in vitro development of mouse embryos. This article extracted from M.Sc. thesis. (Narges Navabi).
authors.affiliation: Department of Animal Science, Islamic Azad University, Damghan Branch, Damghan, Iran.
authors.forename: Narges
authors.initials: N
authors.lastname: Nabavi
authors.affiliation: Department of Venomous Animal and Antivenin Production, Razi Vaccine and Serum Research Institute, Karaj, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Todehdehghan
authors.affiliation: Department of Animal Science, Islamic Azad University, Damghan Branch, Damghan, Iran.
authors.forename: Abdollhossein
authors.initials: A
authors.lastname: Shiravi
keywords: Caffeine; Early embryo.; In-vitro fertilization; Mice; Spermatozoa