title: In vitroeffect of molluscan hemocyanins on CAL-29 and T-24 bladder cancer cell lines.
abstract: The aim of this study was to investigate the anti-tumor effects of molluscan hemocyanins (Hcs) isolated from the marine snailRapana venosa(RvH) and the garden snailHelix lucorum(HlH) on human bladder cancer cell lines. The antitumor effect of the native molecules of the above-mentioned Hcs and their subunits were examined in comparison to keyhole limpet hemocyanin (KLH), which is the most thoroughly studied Hc. The experiments were conducted using 2 human bladder cancer cell lines: CAL-29 and T-24. Doxorubicin hydrochloride (DOX) and mitomycin-C (MIT-C), which are routinely used in clinical practice to treat bladder cancer, were used for comparison. The viability of the 2 bladder cancer cell lines, used at a concentration of 20,000 cells/well, was measured by WST-1 assay at 24, 48 and 72 h after treatment with the above-mentioned Hcs and their isoforms at a concentration ranging from 0.8 to 500μg/ml. A direct growth inhibitory effect on the tumor cells was observed mainly after treatment with the native molecule of HlH and the structural subunit, RvH1, at a concentration of 500μg/ml. The native molecule of RvH exhibited an efficacy similar to that of KLH. However, the observed growth inhibitory effect of HlH was superior to that observed for KLH and RvH, when used at the same concentration. These findings demonstrate the antitumor effect of other Hcs, apart from KLH. Our data suggest that the native molecule of HlH and the subunit, RvH1, are alternative candidates for the treatment of human superficial bladder cancer.
authors.affiliation: Department of Medical Genetics, Medical University of Sofia, Sofia 1431;
authors.forename: Olga
authors.initials: O
authors.lastname: Boyanova
authors.affiliation: Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Sofia 1113, Bulgaria ;
authors.forename: Pavlina
authors.initials: P
authors.lastname: Dolashka
authors.affiliation: Department of Medical Genetics, Medical University of Sofia, Sofia 1431;
authors.forename: Draga
authors.initials: D
authors.lastname: Toncheva
authors.affiliation: Institute for Cell Biology, Department of Immunology, University of Tübingen, D-72076 Tübingen, Germany.
authors.forename: Hans-Georg
authors.initials: HG
authors.lastname: Rammensee
authors.affiliation: Institute for Cell Biology, Department of Immunology, University of Tübingen, D-72076 Tübingen, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Stevanović
keywords: bladder cancer; cell line; hemocyanin; therapy