title: Rad GTPase inhibits the NFκB pathway through interacting with RelA/p65 to impede its DNA binding and target gene transactivation.
abstract: Rad is a Ras-related small GTPase shown to inhibit cancer cell migration, and its expression is frequently lost in lung cancer cells. Here we provide evidence that Rad can negatively regulate the NFκB pathway. Overexpressing Rad in cells lowered both the basal and TNFα-stimulated transcriptional activity of NFκB. Compared with control cells, Rad-overexpressing cells displayed more cytoplasmic distribution of the NFκB subunit RelA/p65, while Rad-knockdown cells had higher levels of nuclear RelA/p65. Depleting Rad did not affect the kinetics of TNFα-induced IκB degradation, suggesting that Rad-mediated regulation of NFκB was through an IκB-independent mechanism. Expression of a nucleus-localized mutant Rad was sufficient to inhibit the NFκB transcriptional activity, whereas expressing the scaffolding protein 14-3-3γ to retain Rad in the cytoplasm alleviated the suppressive effect of Rad on NFκB. GST pull-down assays showed that Rad could directly bind to RelA/p65, and co-immunoprecipitation demonstrated that the Rad-p65 interaction primarily occurred in the nucleus. Adding Rad-containing nuclear extracts or purified GST-Rad in the electrophoretic mobility shift assays dose-dependently decreased the binding of RelA/p65 to an oligonucleotide probe containing the NFκB response element, suggesting that Rad may directly impede the interaction between RelA/p65 and DNA. Rad depletion altered the expression of an array of NFκB target genes, including upregulating MMP9. Knockdown of Rad expression in cells increased both basal and TNFα-stimulated MMP9 activities and cell invasion. Collectively, our results disclose a novel role of nuclear Rad in inhibiting the NFκB pathway function.
authors.affiliation: Institute of Biochemistry and Molecular Biology, National Yang-Ming University, No. 155, Section 2, Li-Nong Street, Taipei 11221, Taiwan. Electronic address: BYHsiao0916@gmail.com.
authors.forename: Bo-Yuan
authors.initials: BY
authors.lastname: Hsiao
authors.affiliation: Institute of Biochemistry and Molecular Biology, National Yang-Ming University, No. 155, Section 2, Li-Nong Street, Taipei 11221, Taiwan.
authors.forename: Tsun-Kai
authors.initials: TK
authors.lastname: Chang
authors.affiliation: Institute of Biochemistry and Molecular Biology, National Yang-Ming University, No. 155, Section 2, Li-Nong Street, Taipei 11221, Taiwan.
authors.forename: I-Ting
authors.initials: IT
authors.lastname: Wu
authors.affiliation: Institute of Biochemistry and Molecular Biology, National Yang-Ming University, No. 155, Section 2, Li-Nong Street, Taipei 11221, Taiwan. Electronic address: meychen@ym.edu.tw.
authors.forename: Mei-Yu
authors.initials: MY
authors.lastname: Chen
mesh_terms: D048948:14-3-3 Proteins / Q000096:biosynthesis; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D024202:Electrophoretic Mobility Shift Assay; D006801:Humans; D051550:I-kappa B Kinase / Q000378:metabolism; D008175:Lung Neoplasms / Q000235:genetics / Q000473:pathology*; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis; D009361:Neoplasm Invasiveness / Q000235:genetics; D011485:Protein Binding; D034622:RNA Interference; D034741:RNA, Small Interfering; D020218:Response Elements / Q000235:genetics; D051996:Transcription Factor RelA / Q000096:biosynthesis / Q000378:metabolism*; D015533:Transcriptional Activation; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*; D018631:ras Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*
chemical_list: D048948:14-3-3 Proteins; D004268:DNA-Binding Proteins; C498143:RELA protein, human; D034741:RNA, Small Interfering; C084355:RRAD protein, human; D051996:Transcription Factor RelA; D014409:Tumor Necrosis Factor-alpha; D051550:I-kappa B Kinase; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9; D018631:ras Proteins
keywords: GTPase; NFκB; Rad; RelA/p65; TNFα