title: Antibacterial effects of afzelin isolated from Cornus macrophylla on Pseudomonas aeruginosa, a leading cause of illness in immunocompromised individuals.
abstract: The crude ethyl acetate extract of the leaves of Cornus macrophylla showed antibacterial activity against Pseudomonas aeruginosa, a leading cause of illness in immunocompromised individuals. Bioactivity-guided separation led to the isolation of kaempferol 3-O-α-L-rhamnopyranoside (afzelin). The structure was determined based on evaluation of its spectroscopic (UV, MS, and NMR) data. The minimum inhibitory concentration (MIC) of afzelin against Pseudomonas aeruginosa was found to be 31 µg/mL. In addition, the results indicated that a hydroxyl group at C3 of the C-ring of the flavone skeleton and the rhamnose group may act as a negative factor and an enhancing factor, respectively, in the antibacterial activities of afzelin.
authors.affiliation: Department of Life Science, Gachon University, San 65, Bokjeong-Dong, Sujeong-Gu, Seongnam-Si, 461-701 Gyeonggi-Do, Korea. leesaye@gachon.ac.kr.
authors.forename: Sang Yeol
authors.initials: SY
authors.lastname: Lee
authors.affiliation: Department of Dermatological Health Management, College of Health Science, Eulji University, Seongna-Si, 461-713 Gyeonggi-Do, Korea. yjso@eulji.ac.kr.
authors.forename: Young-Jin
authors.initials: YJ
authors.lastname: So
authors.affiliation: Department of Dermatological Health Management, College of Health Science, Eulji University, Seongna-Si, 461-713 Gyeonggi-Do, Korea. msshin@eulji.ac.kr.
authors.forename: Moon Sam
authors.initials: MS
authors.lastname: Shin
authors.affiliation: Department of Genetic Engineering, Sungkyunkwan University, Suwon-Si, 440-746 Gyeonggi-Do, Korea. jaecho@skku.edu.
authors.forename: Jae Youl
authors.initials: JY
authors.lastname: Cho
authors.affiliation: Department of Dermatological Health Management, College of Health Science, Eulji University, Seongna-Si, 461-713 Gyeonggi-Do, Korea. jslee@eulji.ac.kr.
authors.forename: Jongsung
authors.initials: J
authors.lastname: Lee
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D029768:Cornus / Q000737:chemistry*; D006801:Humans; D016867:Immunocompromised Host; D008362:Mannosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000737:chemistry*; D044945:Proanthocyanidins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D011552:Pseudomonas Infections / Q000276:immunology / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D008362:Mannosides; D010936:Plant Extracts; D044945:Proanthocyanidins; C477954:afzelin