title: Crystal structure of DNA polymerase β with DNA containing the base lesion spiroiminodihydantoin in a templating position.
abstract: The first high-resolution crystal structure of spiroiminodihydantoin (dSp1) was obtained in the context of the DNA polymerase β active site and reveals two areas of significance. First, the structure verifies the recently determined S configuration at the spirocyclic carbon. Second, the distortion of the DNA duplex is similar to that of the single-oxidation product 8-oxoguanine. For both oxidized lesions, adaptation of the syn conformation results in similar backbone distortions in the DNA duplex. The resulting conformation positions the dSp1 A-ring as the base-pairing face whereas the B-ring of dSp1 protrudes into the major groove.
authors.affiliation: Department of Microbiology and Molecular Genetics, University of Vermont , Stafford Hall, 95 Carrigan Drive, Burlington, Vermont 05405, United States.
authors.forename: Brian E
authors.initials: BE
authors.lastname: Eckenroth
authors.forename: Aaron M
authors.initials: AM
authors.lastname: Fleming
authors.forename: Joann B
authors.initials: JB
authors.lastname: Sweasy
authors.forename: Cynthia J
authors.initials: CJ
authors.lastname: Burrows
authors.forename: Sylvie
authors.initials: S
authors.lastname: Doublié
mesh_terms: D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D019951:DNA Polymerase beta / Q000737:chemistry* / Q000378:metabolism; D006151:Guanosine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D008958:Models, Molecular; D013141:Spiro Compounds / Q000737:chemistry / Q000378:metabolism*; D013698:Templates, Genetic
chemical_list: D013141:Spiro Compounds; C408822:spiroiminodihydantoin; D006151:Guanosine; D004247:DNA; D019951:DNA Polymerase beta