title: MiRNA expression profile for the human gastric antrum region using ultra-deep sequencing.
abstract: BACKGROUND
  MicroRNAs are small non-coding nucleotide sequences that regulate gene expression. These structures are fundamental to several biological processes, including cell proliferation, development, differentiation and apoptosis. Identifying the expression profile of microRNAs in healthy human gastric antrum mucosa may help elucidate the miRNA regulatory mechanisms of the human stomach.
  
  
  RESULTS
  A small RNA library of stomach antrum tissue was sequenced using high-throughput SOLiD sequencing technology. The total read count for the gastric mucosa antrum region was greater than 618,000. After filtering and aligning using with MirBase, 148 mature miRNAs were identified in the gastric antrum tissue, totaling 3,181 quality reads; 63.5% (2,021) of the reads were concentrated in the eight most highly expressed miRNAs (hsa-mir-145, hsa-mir-29a, hsa-mir-29c, hsa-mir-21, hsa-mir-451a, hsa-mir-192, hsa-mir-191 and hsa-mir-148a). RT-PCR validated the expression profiles of seven of these highly expressed miRNAs and confirmed the sequencing results obtained using the SOLiD platform.
  
  
  CONCLUSIONS
  In comparison with other tissues, the antrum's expression profile was unique with respect to the most highly expressed miRNAs, suggesting that this expression profile is specific to stomach antrum tissue. The current study provides a starting point for a more comprehensive understanding of the role of miRNAs in the regulation of the molecular processes of the human stomach.
authors.affiliation: Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brasil.
authors.forename: Fabiano Cordeiro
authors.initials: FC
authors.lastname: Moreira
authors.affiliation: Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brasil; Hospital Universitário João de Barros Barreto, Universidade Federal do Pará, Belém, PA, Brasil.
authors.forename: Monica
authors.initials: M
authors.lastname: Assumpção
authors.affiliation: Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brasil; Universidade Federal Rural da Amazônia, Capanema, PA, Brasil.
authors.forename: Igor G
authors.initials: IG
authors.lastname: Hamoy
authors.affiliation: Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brasil.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Darnet
authors.affiliation: Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brasil; Núcleo de Pesquisa em Oncologia, Universidade Federal do Pará, Belém, PA, Brasil.
authors.forename: Rommel
authors.initials: R
authors.lastname: Burbano
authors.affiliation: Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brasil; Núcleo de Pesquisa em Oncologia, Universidade Federal do Pará, Belém, PA, Brasil.
authors.forename: André
authors.initials: A
authors.lastname: Khayat
authors.affiliation: Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brasil.
authors.forename: André Nicolau
authors.initials: AN
authors.lastname: Gonçalves
authors.affiliation: Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brasil.
authors.forename: Dayse O
authors.initials: DO
authors.lastname: Alencar
authors.affiliation: Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brasil; Secretaria Estadual de Saúde do Estado do Pará, Belém, PA, Brasil.
authors.forename: Aline
authors.initials: A
authors.lastname: Cruz
authors.affiliation: Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brasil.
authors.forename: Leandro
authors.initials: L
authors.lastname: Magalhães
authors.affiliation: Centro Regional de Hemoterapia, Faculdade Medicina Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil.
authors.forename: Wilson
authors.initials: W
authors.lastname: Araújo
authors.affiliation: Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brasil.
authors.forename: Artur
authors.initials: A
authors.lastname: Silva
authors.affiliation: Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brasil; Núcleo de Pesquisa em Oncologia, Universidade Federal do Pará, Belém, PA, Brasil.
authors.forename: Sidney
authors.initials: S
authors.lastname: Santos
authors.affiliation: Núcleo de Pesquisa em Oncologia, Universidade Federal do Pará, Belém, PA, Brasil; Instituto de Ciências da Saúde, Universidade Federal do Pará, Belém, PA, Brasil.
authors.forename: Samia
authors.initials: S
authors.lastname: Demachki
authors.affiliation: Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brasil; Hospital Universitário João de Barros Barreto, Universidade Federal do Pará, Belém, PA, Brasil.
authors.forename: Paulo
authors.initials: P
authors.lastname: Assumpção
authors.affiliation: Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brasil; Núcleo de Pesquisa em Oncologia, Universidade Federal do Pará, Belém, PA, Brasil.
authors.forename: Andrea
authors.initials: A
authors.lastname: Ribeiro-dos-Santos
mesh_terms: D020869:Gene Expression Profiling; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D011706:Pyloric Antrum / Q000378:metabolism*
chemical_list: C546177:MIRN191 microRNA, human; C546783:MIRN192 microRNA, human; C532045:MIRN29a microRNA, human; D035683:MicroRNAs