title: Therapeutic interaction of systemically-administered mesenchymal stem cells with peri-implant mucosa.
abstract: OBJECTIVE
  The objective of this study was to investigate the effect of systemically transplanted mesenchymal stem cells (MSCs) on the peri-implant epithelial sealing around dental implants.
  
  
  METHODS
  MSCs were isolated from bone marrow of donor rats and expanded in culture. After recipient rats received experimental titanium dental implants in the bone sockets after extraction of maxillary right first molars, donor rat MSCs were intravenously transplanted into the recipient rats.
  
  
  RESULTS
  The injected MSCs were found in the oral mucosa surrounding the dental implants at 24 hours post-transplantation. MSC transplantation accelerated the formation of the peri-implant epithelium (PIE)-mediated mucosa sealing around the implants at an early stage after implantation. Subsequently, enhanced deposition of laminin-332 was found along the PIE-implant interface at 4 weeks after the replacement. We also observed enhanced attachment and proliferation of oral mucous epithelial cells.
  
  
  CONCLUSIONS
  Systemically transplanted MSCs might play a critical role in reinforcing the epithelial sealing around dental implants.
authors.affiliation: Section of Implant and Rehabilitative Dentistry, Division of Oral Rehabilitation, Faculty of Dental Science Kyushu University, Fukuoka, Japan.
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Kondo
authors.affiliation: Section of Implant and Rehabilitative Dentistry, Division of Oral Rehabilitation, Faculty of Dental Science Kyushu University, Fukuoka, Japan.
authors.forename: Ikiru
authors.initials: I
authors.lastname: Atsuta
authors.affiliation: Section of Implant and Rehabilitative Dentistry, Division of Oral Rehabilitation, Faculty of Dental Science Kyushu University, Fukuoka, Japan.
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Ayukawa
authors.affiliation: Department of Molecular Cell Biology and Oral Anatomy, Graduate School of Dental Science Kyushu University, Fukuoka, Japan.
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Yamaza
authors.affiliation: Section of Implant and Rehabilitative Dentistry, Division of Oral Rehabilitation, Faculty of Dental Science Kyushu University, Fukuoka, Japan.
authors.forename: Yuri
authors.initials: Y
authors.lastname: Matsuura
authors.affiliation: Section of Implant and Rehabilitative Dentistry, Division of Oral Rehabilitation, Faculty of Dental Science Kyushu University, Fukuoka, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Furuhashi
authors.affiliation: Section of Implant and Rehabilitative Dentistry, Division of Oral Rehabilitation, Faculty of Dental Science Kyushu University, Fukuoka, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Tsukiyama
authors.affiliation: Section of Implant and Rehabilitative Dentistry, Division of Oral Rehabilitation, Faculty of Dental Science Kyushu University, Fukuoka, Japan.
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Koyano
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D015815:Cell Adhesion Molecules; D002454:Cell Differentiation; D002469:Cell Separation; D018920:Coculture Techniques; D015921:Dental Implants; D004847:Epithelial Cells / Q000166:cytology; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D009061:Mouth Mucosa / Q000166:cytology*; D039902:Multipotent Stem Cells / Q000166:cytology; D055647:Rats, Transgenic; D017208:Rats, Wistar; D014081:Tooth Extraction; D014945:Wound Healing; D000097794:Kalinin
chemical_list: D015815:Cell Adhesion Molecules; D015921:Dental Implants; D049452:Green Fluorescent Proteins; D000097794:Kalinin