title: The use of autologous fibrin as a scaffold for cultivating autologous conjunctiva in the treatment of conjunctival defect.
abstract: The purpose of this study was to compare the use of autologous fibrin to human amniotic membrane (HAM) as a scaffold in cultivating autologous conjunctiva for transplantation in treatment of conjunctival defect. An experimental study was performed using 18 adult New Zealand white strain rabbits which were divided into 3 groups. Each group consists of 6 rabbits. The conjunctiva on the temporal site was excised to create a conjunctival epithelial defect. The excised area in the Group 1 was transplanted with autologous conjunctiva cultivated on autologous fibrin; Group 2 was transplanted with autologous conjunctiva cultivated on HAM and Group 3 was left bare. The rabbits were followed up at regular intervals until 6 weeks. The mean period of complete conjunctival epithelization was 11.50 ± 8.22 days for the autologous fibrin group, 15.33 ± 11.80 days for the HAM group and 25.33 ± 5.32 days in the bare sclera group. The epithelization rate for the autologous fibrin group was faster compared to the other two groups. However all the results were not statistically significant (p value >0.05). There were no postoperative complications noted during the follow up. Autologous fibrin is comparable to HAM as a scaffold for cultivation of conjunctiva in the treatment of conjunctival defect.
authors.affiliation: Department of Ophthalmology, Faculty of Medicine, Universiti Kebangsaan Malaysia Medical Center, Jalan Yaakob Latif, Bandar Tun Razak, Cheras, 56000, Kuala Lumpur, Malaysia.
authors.forename: M K
authors.initials: MK
authors.lastname: Safinaz
authors.forename: A G
authors.initials: AG
authors.lastname: Norzana
authors.forename: M H
authors.initials: MH
authors.lastname: Hairul Nizam
authors.forename: A R
authors.initials: AR
authors.lastname: Ropilah
authors.forename: H A
authors.initials: HA
authors.lastname: Faridah
authors.forename: K H
authors.initials: KH
authors.lastname: Chua
authors.forename: B H I
authors.initials: BH
authors.lastname: Ruszymah
authors.forename: C H
authors.initials: CH
authors.lastname: Jemaima
mesh_terms: D000650:Amnion; D000818:Animals; D064592:Autografts; D049109:Cell Proliferation; D003228:Conjunctiva / Q000166:cytology / Q000637:transplantation*; D003229:Conjunctival Diseases / Q000601:surgery*; D004195:Disease Models, Animal; D004847:Epithelial Cells / Q000166:cytology; D005337:Fibrin; D006084:Graft Rejection / Q000517:prevention & control; D066298:In Vitro Techniques; D011817:Rabbits; D046509:Tissue Culture Techniques; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D005337:Fibrin