title: Packed-fiber solid-phase extraction coupled with high performance liquid chromatography-tandem mass spectrometry for determination of diethylstilbestrol, hexestrol, and dienestrol residues in milk products.
abstract: A sensitive analytical method based on packed-fiber solid-phase extraction and high performance liquid chromatography-tandem mass spectrometry (PF SPE-HPLC-MS/MS) has been developed for determination of three synthetic stilbenes in milk. The stilbenes are extracted with acetonitrile, using sodium chloride, and purified with PF SPE using a cartridge containing electrospun polystyrene nanofibers. Parameters affecting the efficiency of PF SPE, such as pH and amount of salt, were optimized. Under optimal conditions, the limits of detection and quantification were 5-13pg/g and 15-37pg/g, respectively. Absolute recoveries varied between 60% and 85% at three different levels. The method was successfully applied for the determination of estrogenic stilbenes in a total of 69 milk samples. The method is sensitive and cost-effective in stilbene detection, and has potential in quality control of dairy products.
authors.affiliation: State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, PR China; Nanjing Bureau of Quality and Technical Supervision, Nanjing, PR China.
authors.forename: Wen-Yan
authors.initials: WY
authors.lastname: Hu
authors.affiliation: Key Laboratory of Child Development and Learning Science of Ministry of Education, Research Center for Learning Science, Southeast University, Nanjing, PR China.
authors.forename: Xue-Jun
authors.initials: XJ
authors.lastname: Kang
authors.affiliation: Nanjing Bureau of Quality and Technical Supervision, Nanjing, PR China.
authors.forename: Chi
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Nanjing Bureau of Quality and Technical Supervision, Nanjing, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.affiliation: Nanjing Bureau of Quality and Technical Supervision, Nanjing, PR China.
authors.forename: Rui
authors.initials: R
authors.lastname: Ling
authors.affiliation: State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, PR China. Electronic address: liuehu2011@163.com.
authors.forename: E-Hu
authors.initials: EH
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, PR China. Electronic address: liping2004@126.com.
authors.forename: Ping
authors.initials: P
authors.lastname: Li
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004028:Dienestrol / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D004054:Diethylstilbestrol / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D006589:Hexestrol / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D057230:Limit of Detection; D016014:Linear Models; D008892:Milk / Q000737:chemistry*; D015203:Reproducibility of Results; D052616:Solid Phase Extraction / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D006589:Hexestrol; D004054:Diethylstilbestrol; D004028:Dienestrol
keywords: Liquid dairy products; Packed-fiber solid-phase extraction and high performance liquid chromatography–tandem mass spectrometry; Stilbenes