title: Proteome analysis of Escherichia coli periplasmic proteins in response to over-expression of recombinant human interferon α2b.
abstract: The periplasmic proteome of recombinant E. coli cells expressing human interferon-α2b (INF-α2b) was analysed by 2D-gel electrophoresis to find the most altered proteins. Of some unique up- and down-regulated proteins in the proteome, ten were identified by MS. The majority of the proteins belonged to the ABC transporter protein family. Other affected proteins were ones involved in the regulation of transcription such as DNA-binding response regulator, stress-related proteins and ecotin. Thus, the production of INF-α2b acts as a stress on the cells and results in the induction of various transporters and stress related proteins.
authors.affiliation: Department of Cell and Molecular Biology, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.
authors.forename: Somayyeh
authors.initials: S
authors.lastname: Heidary
authors.forename: Raha Abdul
authors.initials: RA
authors.lastname: Rahim
authors.forename: Samira
authors.initials: S
authors.lastname: Eissazadeh
authors.forename: Hassan
authors.initials: H
authors.lastname: Moeini
authors.forename: Adam Leow Thean
authors.initials: AL
authors.lastname: Chor
authors.forename: Mohd Puad
authors.initials: MP
authors.lastname: Abdullah
mesh_terms: D015180:Electrophoresis, Gel, Two-Dimensional; D004926:Escherichia coli / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000032:analysis*; D006801:Humans; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000235:genetics / Q000378:metabolism*; D013058:Mass Spectrometry; D033903:Periplasmic Proteins / Q000032:analysis*; D020543:Proteome / Q000032:analysis*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D029968:Escherichia coli Proteins; D000077190:Interferon alpha-2; D016898:Interferon-alpha; D033903:Periplasmic Proteins; D020543:Proteome; D011994:Recombinant Proteins