title: Mechanical properties, biological activity and protein controlled release by poly(vinyl alcohol)-bioglass/chitosan-collagen composite scaffolds: a bone tissue engineering applications.
abstract: In the present study, composite scaffolds made with different weight ratios (0.5:1, 1:1 and 2:1) of bioactive glass (15Ca:80Si:5P) (BG)/polyvinyl alcohol (PVA) (PVABG) and chitosan (Chi)/collagen (Col) (ChiCol) were prepared by three mechanical freeze-thaw followed by freeze-drying to obtain the porous scaffolds. The mechanical properties and the in vitro biocompatibility of the composite scaffolds to simulated body fluid (SBF) and to rat osteoblast-like UMR-106 cells were investigated. The results from the studies indicated that the porosity and compressive strength were controlled by the weight ratio of PVABG:ChiCol. The highest compressive modulus of the composites made was 214.64 MPa which was for the 1:1 weight ratio PVABG:ChiCol. Mineralization study in SBF showed the formation of apatite crystals on the PVABG:ChiCol surface after 7 days of incubation. In vitro cell availability and proliferation tests confirmed the osteoblast attachment and growth on the PVABG:ChiCol surface. MTT and ALP tests on the 1:1 weight ratio PVABG:ChiCol composite indicated that the UMR-106 cells were viable. Alkaline phosphatase activity was found to increase with increasing culturing time. In addition, we showed the potential of PVABG:ChiCol drug delivery through PBS solution studies. 81.14% of BSA loading had been achieved and controlled release for over four weeks was observed. Our results indicated that the PVABG:ChiCol composites, especially the 1:1 weight ratio composite exhibited significantly improved mechanical, mineral deposition, biological properties and controlled release. This made them potential candidates for bone tissue engineering applications.
authors.affiliation: Department of Physics, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand. Electronic address: fsciwpp@ku.ac.th.
authors.forename: Weeraphat
authors.initials: W
authors.lastname: Pon-On
authors.affiliation: Center of Calcium and Bone Research (COCAB), Faculty of Science, Mahidol University, Thailand; Department of Physiology, Faculty of Science, Mahidol University, Thailand.
authors.forename: Narattaphol
authors.initials: N
authors.lastname: Charoenphandhu
authors.affiliation: Center of Calcium and Bone Research (COCAB), Faculty of Science, Mahidol University, Thailand; Department of Physiology, Faculty of Science, Mahidol University, Thailand.
authors.forename: Jarinthorn
authors.initials: J
authors.lastname: Teerapornpuntakit
authors.affiliation: Center of Calcium and Bone Research (COCAB), Faculty of Science, Mahidol University, Thailand; Department of Physiology, Faculty of Science, Mahidol University, Thailand.
authors.forename: Jirawan
authors.initials: J
authors.lastname: Thongbunchoo
authors.affiliation: Center of Calcium and Bone Research (COCAB), Faculty of Science, Mahidol University, Thailand; Department of Physiology, Faculty of Science, Mahidol University, Thailand.
authors.forename: Nateetip
authors.initials: N
authors.lastname: Krishnamra
authors.affiliation: ThEP Center, Commission of Higher Education, 328 Si Ayutthaya Rd., Thailand; Department of Materials Science, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
authors.forename: I-Ming
authors.initials: IM
authors.lastname: Tang
mesh_terms: D000327:Adsorption; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001842:Bone and Bones / Q000187:drug effects*; D002113:Calcification, Physiologic / Q000187:drug effects; D002417:Cattle; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002516:Ceramics / Q000494:pharmacology*; D048271:Chitosan / Q000494:pharmacology*; D003094:Collagen / Q000494:pharmacology*; D019245:Compressive Strength / Q000187:drug effects; D055595:Mechanical Phenomena / Q000187:drug effects*; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects; D011142:Polyvinyl Alcohol / Q000494:pharmacology*; D051381:Rats; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry; D014961:X-Ray Diffraction
chemical_list: C022107:Bioglass; D012710:Serum Albumin, Bovine; D011142:Polyvinyl Alcohol; D003094:Collagen; D048271:Chitosan; D000469:Alkaline Phosphatase
keywords: Bioactive glass; Chitosan; Collagen; Mechanical properties; Poly(vinyl alcohol)