title: New ceramide from the aerial part of Tinospora oblongifolia with cytotoxic activities.
abstract: A new ceramide, 2,3-dihydroxy-N-[(2S,3S,4R)-1,3,4-trihydroxyicosan-2-yl[tetracosanamide (1) along with four known compounds: 2-(4'-hydroxyphenyl)-ethyl lignocerate (2), docosyl-3,4-dihydroxy-trans-cinnamate (3), β-sitosterol (4) and β-sitosterol glycoside (5) were isolated from Tinospora oblongifolia (Menispermaceae). Their structures were determined on the basis of spectroscopic methods, mass spectrometry analysis as well as chemical transformation and by comparing their physical and spectral data with those reported in the literature. Compound 1 exhibited strong cytotoxic activity against KB cells with IC50 = 3.4 μM although less than that of camptothecin IC50 = 0.3 μM (positive control).
authors.affiliation: a Department of Chemistry , School of Biological and Physical Sciences, Maseno University , Maseno , Kenya.
authors.forename: Fidelis
authors.initials: F
authors.lastname: Samita
authors.forename: Charles O
authors.initials: CO
authors.lastname: Ochieng
authors.forename: Philip O
authors.initials: PO
authors.lastname: Owuor
authors.forename: Lawrence A O
authors.initials: LA
authors.lastname: Manguro
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D002166:Camptothecin / Q000494:pharmacology; D002518:Ceramides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D020128:Inhibitory Concentration 50; D007624:KB Cells; D007630:Kenya; D015394:Molecular Structure; D018547:Plant Stems / Q000737:chemistry; D012855:Sitosterols / Q000737:chemistry / Q000302:isolation & purification; D031610:Tinospora / Q000737:chemistry*
chemical_list: C000591772:2,3-dihydroxy-N-((2S,3S,4R)-1,3,4-trihydroxyicosan-2-yl)tetracosanamide; D000972:Antineoplastic Agents, Phytogenic; D002518:Ceramides; D012855:Sitosterols; C025473:gamma-sitosterol; D002166:Camptothecin
keywords: Menispermaceae; Tinospora oblongifolia; ceramide; cytotoxicity