title: A maize spermine synthase 1 PEST sequence fused to the GUS reporter protein facilitates proteolytic degradation.
abstract: Polyamines are low molecular weight aliphatic compounds involved in various biochemical, cellular and physiological processes in all organisms. In plants, genes involved in polyamine biosynthesis and catabolism are regulated at transcriptional, translational, and posttranslational level. In this research, we focused on the characterization of a PEST sequence (rich in proline, glutamic acid, serine, and threonine) of the maize spermine synthase 1 (ZmSPMS1). To this aim, 123 bp encoding 40 amino acids of the C-terminal region of the ZmSPMS1 enzyme containing the PEST sequence were fused to the GUS reporter gene. This fusion was evaluated in Arabidopsis thaliana transgenic lines and onion monolayers transient expression system. The ZmSPMS1 PEST sequence leads to specific degradation of the GUS reporter protein. It is suggested that the 26S proteasome may be involved in GUS::PEST fusion degradation in both onion and Arabidopsis. The PEST sequences appear to be present in plant spermine synthases, mainly in monocots.
authors.affiliation: División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica A. C., 78216 San Luis Potosí, SLP, Mexico.
authors.forename: Israel
authors.initials: I
authors.lastname: Maruri-López
authors.affiliation: Facultad de Ciencias, Universidad Autónoma de San Luis Potosí, Av. Salvador Nava s/n, Zona Universitaria, 78290 San Luis Potosí, SLP, Mexico.
authors.forename: Margarita
authors.initials: M
authors.lastname: Rodríguez-Kessler
authors.affiliation: División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica A. C., 78216 San Luis Potosí, SLP, Mexico.
authors.forename: Aída Araceli
authors.initials: AA
authors.lastname: Rodríguez-Hernández
authors.affiliation: División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica A. C., 78216 San Luis Potosí, SLP, Mexico.
authors.forename: Alicia
authors.initials: A
authors.lastname: Becerra-Flora
authors.affiliation: Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Av. Universidad 2001, Colonia Chamilpa, 62210 Cuernavaca, Morelos, Mexico.
authors.forename: Juan Elías
authors.initials: JE
authors.lastname: Olivares-Grajales
authors.affiliation: División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica A. C., 78216 San Luis Potosí, SLP, Mexico. Electronic address: jbremont@ipicyt.edu.mx.
authors.forename: Juan Francisco
authors.initials: JF
authors.lastname: Jiménez-Bremont
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D015153:Blotting, Western; D015853:Cysteine Proteinase Inhibitors / Q000494:pharmacology; D017343:Genes, Plant / Q000235:genetics; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism*; D007976:Leupeptins / Q000494:pharmacology; D008969:Molecular Sequence Data; D019697:Onions / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism; D059748:Proteolysis; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013097:Spermine Synthase / Q000235:genetics / Q000378:metabolism*; D003313:Zea mays / Q000235:genetics / Q000378:metabolism*
chemical_list: D015853:Cysteine Proteinase Inhibitors; D007976:Leupeptins; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D013097:Spermine Synthase; D005966:Glucuronidase; D046988:Proteasome Endopeptidase Complex; C061553:ATP dependent 26S protease; C072553:benzyloxycarbonylleucyl-leucyl-leucine aldehyde
keywords: 26S proteasome; Aminopropyl transferases; PEST; Polyamines; Spermine synthase