title: Biodistribution of locally or systemically transplantedosteoblast-like cells.
abstract: OBJECTIVE
  In order to ensure safety of the cell-based therapy for bone regeneration, we examined in vivo biodistribution of locally or systemically transplanted osteoblast-like cells generated from bone marrow (BM) derived mononuclear cells.
  
  
  METHODS
  BM cells obtained from a total of 13 Sprague-Dawley (SD) green fluorescent protein transgenic (GFP-Tg) rats were culture-expanded in an osteogenic differentiation medium for three weeks. Osteoblast-like cells were then locally transplanted with collagen scaffolds to the rat model of segmental bone defect. Donor cells were also intravenously infused to the normal Sprague-Dawley (SD) rats for systemic biodistribution. The flow cytometric and histological analyses were performed for cellular tracking after transplantation.
  
  
  RESULTS
  Locally transplanted donor cells remained within the vicinity of the transplantation site without migrating to other organs. Systemically administered large amounts of osteoblast-like cells were cleared from various organ tissues within three days of transplantation and did not show any adverse effects in the transplanted rats.
  
  
  CONCLUSIONS
  We demonstrated a precise assessment of donor cell biodistribution that further augments prospective utility of regenerative cell therapy. Cite this article: Bone Joint Res 2014;3:76-81.
authors.affiliation: Nagoya University Hospital, Centerfor Advanced Medicine and Clinical Research, 65 Tsurumai, Showa-ku, Nagoya, Aichi466-8550, Japan.
authors.forename: Y T
authors.initials: YT
authors.lastname: Okabe
authors.forename: T
authors.initials: T
authors.lastname: Kondo
authors.forename: K
authors.initials: K
authors.lastname: Mishima
authors.forename: Y
authors.initials: Y
authors.lastname: Hayase
authors.forename: K
authors.initials: K
authors.lastname: Kato
authors.forename: M
authors.initials: M
authors.lastname: Mizuno
authors.forename: N
authors.initials: N
authors.lastname: Ishiguro
authors.forename: H
authors.initials: H
authors.lastname: Kitoh
keywords: Biodistribution; Bone regenerative medicine; Cellular tracking; GFP rats; Osteoblast-like cells; Transplantation