title: Effects of THAP11 on erythroid differentiation and megakaryocytic differentiation of K562 cells.
abstract: Hematopoiesis is a complex process regulated by sets of transcription factors in a stage-specific and context-dependent manner. THAP11 is a transcription factor involved in cell growth, ES cell pluripotency, and embryogenesis. Here we showed that THAP11 was down-regulated during erythroid differentiation but up-regulated during megakaryocytic differentiation of cord blood CD34+ cells. Overexpression of THAP11 in K562 cells inhibited the erythroid differentiation induced by hemin with decreased numbers of benzidine-positive cells and decreased mRNA levels of α-globin (HBA) and glycophorin A (GPA), and knockdown of THAP11 enhanced the erythroid differentiation. Conversely, THAP11 overexpression accelerated the megakaryocytic differentiation induced by phorbol myristate acetate (PMA) with increased percentage of CD41+ cells, increased numbers of 4N cells, and elevated CD61 mRNA levels, and THAP11 knockdown attenuated the megakaryocytic differentiation. The expression levels of transcription factors such as c-Myc, c-Myb, GATA-2, and Fli1 were changed by THAP11 overexpression. In this way, our results suggested that THAP11 reversibly regulated erythroid and megakaryocytic differentiation.
authors.affiliation: Department of Pharmaceutical Engineering, Tianjin University, Tianjin, China; Department of Biochemistry and Molecular Biology, Beijing Institute of Radiation Medicine, Beijing, China.
authors.forename: Xiang-Zhen
authors.initials: XZ
authors.lastname: Kong
authors.affiliation: Department of Biochemistry and Molecular Biology, Beijing Institute of Radiation Medicine, Beijing, China; State Key Laboratory of Proteomics, Beijing, China.
authors.forename: Rong-Hua
authors.initials: RH
authors.lastname: Yin
authors.affiliation: Department of Hematopoietic Stem Cell Transplantation, Affiliated Hospital to Academy of Military Medical Sciences, Beijing, China.
authors.forename: Hong-Mei
authors.initials: HM
authors.lastname: Ning
authors.affiliation: Department of Biochemistry and Molecular Biology, Beijing Institute of Radiation Medicine, Beijing, China.
authors.forename: Wei-Wei
authors.initials: WW
authors.lastname: Zheng
authors.affiliation: Department of Pharmaceutical Engineering, Tianjin University, Tianjin, China.
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Dong
authors.affiliation: Department of Chemistry, Purdue University, West Lafayette, Indiana, United States of America.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Biochemistry and Molecular Biology, Beijing Institute of Radiation Medicine, Beijing, China.
authors.forename: Fei-Fei
authors.initials: FF
authors.lastname: Xu
authors.affiliation: Department of Pulmonary Neoplasms Internal Medicine, Affiliated Hospital to Academy of Military Medicine Sciences, Beijing, China.
authors.forename: Jian-Jie
authors.initials: JJ
authors.lastname: Li
authors.affiliation: Department of Biochemistry and Molecular Biology, Beijing Institute of Radiation Medicine, Beijing, China.
authors.forename: Yi-Qun
authors.initials: YQ
authors.lastname: Zhan
authors.affiliation: Department of Biochemistry and Molecular Biology, Beijing Institute of Radiation Medicine, Beijing, China.
authors.forename: Miao
authors.initials: M
authors.lastname: Yu
authors.affiliation: Department of Biochemistry and Molecular Biology, Beijing Institute of Radiation Medicine, Beijing, China.
authors.forename: Chang-Hui
authors.initials: CH
authors.lastname: Ge
authors.affiliation: Department of Biochemistry and Molecular Biology, Beijing Institute of Radiation Medicine, Beijing, China.
authors.forename: Jian-Hong
authors.initials: JH
authors.lastname: Zhang
authors.affiliation: Department of Biochemistry and Molecular Biology, Beijing Institute of Radiation Medicine, Beijing, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Biochemistry and Molecular Biology, Beijing Institute of Radiation Medicine, Beijing, China; State Key Laboratory of Proteomics, Beijing, China.
authors.forename: Chang-Yan
authors.initials: CY
authors.lastname: Li
authors.affiliation: Department of Pharmaceutical Engineering, Tianjin University, Tianjin, China; Department of Biochemistry and Molecular Biology, Beijing Institute of Radiation Medicine, Beijing, China; State Key Laboratory of Proteomics, Beijing, China.
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Yang
mesh_terms: D018952:Antigens, CD34 / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics*; D041905:Erythroid Cells / Q000166:cytology* / Q000378:metabolism*; D005312:Fetal Blood / Q000166:cytology; D005786:Gene Expression Regulation; D006410:Hematopoiesis / Q000235:genetics*; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000378:metabolism; D006427:Hemin / Q000494:pharmacology; D006801:Humans; D020014:K562 Cells; D008533:Megakaryocytes / Q000166:cytology* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D018952:Antigens, CD34; D012333:RNA, Messenger; D012097:Repressor Proteins; C498470:THAP11 protein, human; D014157:Transcription Factors; D006427:Hemin