title: Assessment of the anti-diarrhea function of compound Chinese herbal medicine Cangpo Oral Liquid.
abstract: BACKGROUND
  Diarrhea is a big problem in piglets. Cangpo Oral Liquid (COL) is a compound of Chinese herbal medicine. The preparation was fed to piglets had diarrheal disease in order to determine its anti-diarrhea activity and potential applications in vivo.
  
  
  METHODS
  The contents of Berberine hydrochloride, Magnolol and Honokiol in COL were performed on HPLC analysis. Organ bath was used to investigate the effect of COL on peristaltic reflexes and peristaltic waves in vitro. And anti-diarrhea activity of COL was evaluated in clinical.
  
  
  RESULTS
  Thin layer chromatography (TLC) and HPLC analyses showed that the contents of Berberine hydrochloride, Magnolol and Honokiol in COL were 970µg/mL, 130µg/mL and 300µg/mL, respectively. Administration of the COL to the organ bath caused a concentration-dependent inhibition of intestinal peristalsis. When the COL concentration in the bath was cumulatively increased, the amplitude and frequency of the peristaltic waves was lowered. The result of clinical efficacy of COL was very effective to diarrheic piglets. COL can possibly inhibit the curve of peristaltic waves in vitro; and clinical trial showed a statistically significant therapeutic effect in vivo.
  
  
  CONCLUSIONS
  In conclusion, COL can be used as an effective therapeutic agent. However, the ingredients, pharmacokinetics and specific signaling pathways of COL need to be further studied.
authors.affiliation: Key Laboratory of Veterinary Pharmaceutical Development of Ministry of Agriculture, Engineering and Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province, Key Laboratory of New Animal Drug Project of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutics Sciences of Chinese Academy of Agricultural Sciences, Lanzhou 730050, Gansu, China.
authors.forename: Xinchao
authors.initials: X
authors.lastname: Xia
authors.affiliation: Key Laboratory of Veterinary Pharmaceutical Development of Ministry of Agriculture, Engineering and Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province, Key Laboratory of New Animal Drug Project of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutics Sciences of Chinese Academy of Agricultural Sciences, Lanzhou 730050, Gansu, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.affiliation: Faculté de Médecine, Université de Bretagne Occidentale, 29238 Brest, France 4 Lanzhou Productivity Promoting Center, Lanzhou 730030. China.
authors.forename: Xu
authors.initials: X
authors.lastname: Niu
authors.affiliation: Key Laboratory of Veterinary Pharmaceutical Development of Ministry of Agriculture, Engineering and Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province, Key Laboratory of New Animal Drug Project of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutics Sciences of Chinese Academy of Agricultural Sciences, Lanzhou 730050, Gansu, China.
authors.forename: Haijun
authors.initials: H
authors.lastname: Wang
authors.affiliation: Institute of Agro-Products Processing Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
authors.forename: Zhiqi
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Key Laboratory of Veterinary Pharmaceutical Development of Ministry of Agriculture, Engineering and Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province, Key Laboratory of New Animal Drug Project of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutics Sciences of Chinese Academy of Agricultural Sciences, Lanzhou 730050, Gansu, China.
authors.forename: Yongming
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Key Laboratory of Veterinary Pharmaceutical Development of Ministry of Agriculture, Engineering and Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province, Key Laboratory of New Animal Drug Project of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutics Sciences of Chinese Academy of Agricultural Sciences, Lanzhou 730050, Gansu, China.
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Qi
authors.affiliation: Key Laboratory of Veterinary Pharmaceutical Development of Ministry of Agriculture, Engineering and Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province, Key Laboratory of New Animal Drug Project of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutics Sciences of Chinese Academy of Agricultural Sciences, Lanzhou 730050, Gansu, China.
authors.forename: Shengyi
authors.initials: S
authors.lastname: Wang
authors.affiliation: Key Laboratory of Veterinary Pharmaceutical Development of Ministry of Agriculture, Engineering and Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province, Key Laboratory of New Animal Drug Project of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutics Sciences of Chinese Academy of Agricultural Sciences, Lanzhou 730050, Gansu, China.
authors.forename: Shixiang
authors.initials: S
authors.lastname: Liu
authors.affiliation: Faculté de Médecine, Université de Bretagne Occidentale, 29238 Brest, France 4 Lanzhou Productivity Promoting Center, Lanzhou 730030. China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Liu
mesh_terms: D000818:Animals; D000930:Antidiarrheals / Q000494:pharmacology / Q000627:therapeutic use*; D001599:Berberine / Q000032:analysis / Q000494:pharmacology / Q000627:therapeutic use; D001713:Biphenyl Compounds / Q000032:analysis / Q000494:pharmacology / Q000627:therapeutic use; D039323:Coptis / Q000737:chemistry*; D003967:Diarrhea / Q000188:drug therapy*; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D005260:Female; D007422:Intestines / Q000187:drug effects; D017705:Lignans / Q000032:analysis / Q000494:pharmacology / Q000627:therapeutic use; D031566:Magnolia / Q000737:chemistry*; D008297:Male; D010528:Peristalsis / Q000187:drug effects*; D008517:Phytotherapy; D013552:Swine; D000498:Allyl Compounds; D010636:Phenols
chemical_list: D000930:Antidiarrheals; D001599:Berberine; D001713:Biphenyl Compounds; D004365:Drugs, Chinese Herbal; D017705:Lignans; C005498:magnolol; C005499:honokiol; D000498:Allyl Compounds; D010636:Phenols
keywords: Cangpo Oral Liquid; herbal; peristalsis; therapeutic effect