title: Multi-matrix assay of the first melatonergic antidepressant agomelatine by combined liquid chromatography-fluorimetric detection and microextraction by packed sorbent.
abstract: A rapid and reliable analytical method has been developed to quantify the melatonergic antidepressant agomelatine in three matrices, and namely saliva, plasma and dried blood spots. The method is based on the use of liquid chromatography with fluorimetric detection exploiting the native fluorescence of agomelatine. For saliva and plasma samples an original microextraction by packed sorbent procedure was implemented obtaining satisfactory extraction yield of the analyte (always higher than 89%) and a good clean-up of the matrices. On the contrary, agomelatine was extracted from dried blood spots by suitable solvent microwave-assisted extraction and injected into chromatographic system. Satisfactory results in terms of sensitivity, linearity, precision, selectivity and accuracy were obtained. Thus, the developed method seems to be suitable for therapeutic drug monitoring of depressed patients under agomelatine therapy.
authors.affiliation: Department of Pharmacy and Biotechnology, Alma Mater Studiorum - University of Bologna, Via Belmeloro 6, 40126 Bologna, Italy.
authors.forename: Maria Addolorata
authors.initials: MA
authors.lastname: Saracino
authors.affiliation: Department of Pharmacy and Biotechnology, Alma Mater Studiorum - University of Bologna, Via Belmeloro 6, 40126 Bologna, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Mercolini
authors.affiliation: Department of Pharmacy and Biotechnology, Alma Mater Studiorum - University of Bologna, Via Belmeloro 6, 40126 Bologna, Italy.
authors.forename: Giorgia
authors.initials: G
authors.lastname: Carbini
authors.affiliation: Department of Pharmacy and Biotechnology, Alma Mater Studiorum - University of Bologna, Via Belmeloro 6, 40126 Bologna, Italy.
authors.forename: Vittorio
authors.initials: V
authors.lastname: Volterra
authors.affiliation: Department of Mental Health, Local Health Unit ASL, C.S.M. Parma Nord - SPOI, Via Suor Maria 3, Colorno, 43052 Parma, Italy.
authors.forename: Antonio Lucio
authors.initials: AL
authors.lastname: Quarta
authors.affiliation: Department of Neuroscience, Section of Psychiatry, University of Genova, Largo Rosanna Benzi 10, 16132 Genova, Italy.
authors.forename: Mario
authors.initials: M
authors.lastname: Amore
authors.affiliation: Department of Pharmacy and Biotechnology, Alma Mater Studiorum - University of Bologna, Via Belmeloro 6, 40126 Bologna, Italy. Electronic address: mariaaugusta.raggi@unibo.it.
authors.forename: Maria Augusta
authors.initials: MA
authors.lastname: Raggi
mesh_terms: D000081:Acetamides / Q000032:analysis* / Q000737:chemistry; D000928:Antidepressive Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D005260:Female; D005470:Fluorometry / Q000379:methods*; D006801:Humans; D008297:Male; D008550:Melatonin / Q000819:agonists*; D008875:Middle Aged; D052617:Solid Phase Microextraction; D009281:Naphthalenes
chemical_list: D000081:Acetamides; D000928:Antidepressive Agents; D008550:Melatonin; C084711:agomelatine; D009281:Naphthalenes
keywords: Agomelatine; Dried blood spots; Liquid chromatography-fluorescence detector; Plasma; Saliva