title: Discordance between changes in the gut microbiota and pathogenicity in a mouse model of spontaneous colitis.
abstract: Under conventional conditions, mice deficient in core 1-derived O-glycans (TM-IEC C1galt1(-/-)), which have a defective mucus layer, experienced spontaneous inflammation of the colon. Analysis of fecal bacterial populations by pyrosequencing of 16S rRNA gene showed that disease in conventional TM-IEC C1galt1(-/-) was associated with shifts in the microbiota manifested by increases in Lactobacillus and Clostridium species, and decreases in unclassified Ruminococcaceae and Lachnospiraceae. Under germ-free (GF) conditions, TM-IEC C1galt1(-/-) presented decreased goblet cells, but did not develop inflammation. Monoassociation of GF TM-IEC C1galt1(-/-) revealed that bacterial species differ significantly in their ability to induce inflammatory changes. Bacteroides thetaiotaomicron caused inflammation, while Lactobacillus johnsonii (enriched during colitis) did not. These observations demonstrate that not all microbiota shifts that correlate with disease contribute to pathogenesis.
authors.affiliation: Department of Food Science and Technology; University of Nebraska; Lincoln, NE USA; Department of Pathology; Johns Hopkins University School of Medicine; Baltimore, MD USA.
authors.forename: Maria Elisa
authors.initials: ME
authors.lastname: Perez-Muñoz
authors.affiliation: Oklahoma Medical Research Foundation; Oklahoma City, OK USA.
authors.forename: Kirk
authors.initials: K
authors.lastname: Bergstrom
authors.affiliation: Oklahoma Medical Research Foundation; Oklahoma City, OK USA.
authors.forename: Vincent
authors.initials: V
authors.lastname: Peng
authors.affiliation: Department of Food Science and Technology; University of Nebraska; Lincoln, NE USA.
authors.forename: Robert
authors.initials: R
authors.lastname: Schmaltz
authors.affiliation: Department of Food Science and Technology; University of Nebraska; Lincoln, NE USA.
authors.forename: Roberto
authors.initials: R
authors.lastname: Jimenez-Cardona
authors.affiliation: Department of Food Science and Technology; University of Nebraska; Lincoln, NE USA.
authors.forename: Nathan
authors.initials: N
authors.lastname: Marsteller
authors.affiliation: Oklahoma Medical Research Foundation; Oklahoma City, OK USA.
authors.forename: Sam
authors.initials: S
authors.lastname: McGee
authors.affiliation: Junior Research Group Intestinal Microbiome; ZIEL-Research Center for Nutrition and Food Sciences; Technische Universität München; Munich, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Clavel
authors.affiliation: Department of Microbiology; Cornell University; Ithaca, NY USA.
authors.forename: Ruth
authors.initials: R
authors.lastname: Ley
authors.affiliation: Oklahoma Medical Research Foundation; Oklahoma City, OK USA.
authors.forename: Jianxin
authors.initials: J
authors.lastname: Fu
authors.affiliation: Oklahoma Medical Research Foundation; Oklahoma City, OK USA.
authors.forename: Lijun
authors.initials: L
authors.lastname: Xia
authors.affiliation: Department of Food Science and Technology; University of Nebraska; Lincoln, NE USA; Department of Pathology; Johns Hopkins University School of Medicine; Baltimore, MD USA.
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Peterson
mesh_terms: D000818:Animals; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000254:growth & development*; D058448:Biota; D003092:Colitis / Q000382:microbiology* / Q000473:pathology; D003106:Colon / Q000382:microbiology* / Q000473:pathology; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004195:Disease Models, Animal; D064806:Dysbiosis / Q000150:complications*; D005700:Galactosyltransferases / Q000172:deficiency*; D006801:Humans; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; C515597:C1galt1 protein, mouse; D005700:Galactosyltransferases
keywords: Akkermasia muciniphila; Bacteroides sartorii; Bacteroides thetaiotaomicron; Clostridium; Lactobacillus johnsonii; core 1 O-glycans deficient mice; dysbiosis; gut microbiota; pyrosequencing; spontaneous colitis