title: Oroxylin A inhibits hypoxia-induced invasion and migration of MCF-7 cells by suppressing the Notch pathway.
abstract: Tumor invasion and migration obstructs the treatment and prognosis of cancer. In this research, we investigated the effect of oroxylin A, a natural compound extracted from Scutellaria radix, the root of Scutellaria baicalensis, on inhibition of the invasion and migration of three different tumor cell lines: MCF-7, DU145, and HepG2. The results suggested that oroxylin A could inhibit hypoxia-induced migration and invasion of the three cell lines mentioned above. To study the detailed mechanisms, studies were carried out on MCF-7 cells and it was found that oroxylin A could regulate the expression of related markers in MCF-7 cells including E-cadherin, N-cadherin, and Vimentin. It was also found that oroxylin A inhibited the hypoxia-induced invasion and migration of MCF-7 cells by suppressing the Notch pathway. Oroxylin A inhibited N1ICD translocating to the nucleus and binding to epithelial-mesenchymal transition-related transcription factor Snail, thus suppressing the invasion and migration of MCF-7 cells. Therefore, oroxylin A is expected to be a promising candidate for antimetastasis treatment through suppression of the hypoxia-induced Notch pathway.
authors.affiliation: aState Key Laboratory of Natural Medicines, Jiangsu Key Laboratory of Carcinogenesis and Intervention bDepartment of Medicinal Chemistry, China Pharmaceutical University, Nanjing, People's Republic of China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Cheng
authors.forename: Kai
authors.initials: K
authors.lastname: Zhao
authors.forename: Guojun
authors.initials: G
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Yao
authors.forename: Qinsheng
authors.initials: Q
authors.lastname: Dai
authors.forename: Hui
authors.initials: H
authors.lastname: Hui
authors.forename: Zhiyu
authors.initials: Z
authors.lastname: Li
authors.forename: Qinglong
authors.initials: Q
authors.lastname: Guo
authors.forename: Na
authors.initials: N
authors.lastname: Lu
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D014408:Biomarkers, Tumor / Q000235:genetics; D015687:Cell Hypoxia; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D058750:Epithelial-Mesenchymal Transition; D005419:Flavonoids / Q000494:pharmacology*; D006801:Humans; D061986:MCF-7 Cells; D009361:Neoplasm Invasiveness; D051881:Receptor, Notch1 / Q000378:metabolism*; D015398:Signal Transduction; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D014408:Biomarkers, Tumor; D005419:Flavonoids; D051881:Receptor, Notch1; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors; C080669:5,7-dihydroxy-6-methoxy-2-phenylchromen-4-one