title: Do progenitor cells from different tissue have the same phenotype?
abstract: The purpose of this work was to validate isolation methods for sheep mesenchymal stem cells (MSC) from different sources and to explore the hypothesis that MSC exhibit markers of the same phenotype independent from tissue source. Cells derived from ovine bone marrow, synovial membrane and adipose tissue were characterized using the following markers: CD44, CD45, CD11b and MHC-I. The isolated MSC were cultivated, went through osteogenic, chondrogenic and adipogenic differentiation, and were characterized by flow cytometry using mouse anti-ovine CD44, CD45 and MHC-I monoclonal antibody (mAb), and mouse anti-bovine CD11b mAb. Ovine MSC from all three sources differentiated under chondorgenic, osteogenic and adipogenic conditions. Also, MSC from the three tissues were found to express CD44 and MHC-I but lack of CD11b and CD45. The results obtained revealed that our isolation methods for the different tissues tested are valid and that MSC from the three sources studied have same immunophenotic characteristics.
authors.affiliation: University College of London, Institute of Orhopaedics and Musculoskeletal Science, Royal National Orthopaedics Hospital, Brockley Hill, HA4 7LP, Stanmore, United Kingdom. Electronic address: r.godoy@ucl.ac.uk.
authors.forename: Roberta F
authors.initials: RF
authors.lastname: Godoy
authors.affiliation: Department of Veterinary Surgery and Anesthesiology, School of Veterinary Medicine and Animal Science, São Paulo State University, Distrito de Rubião Junior, S/N. Caixa Postal 560, CEP 18618-970, Botucatu, São Paulo-SP, Brazil.
authors.forename: Ana Liz G
authors.initials: AL
authors.lastname: Alves
authors.affiliation: Royal Veterinary College, Hawkshead Lane, North Mimms, AL9 7TA, Hatfield, United Kingdom.
authors.forename: Amanda J
authors.initials: AJ
authors.lastname: Gibson
authors.affiliation: Veterinary Teaching Hospital, University of Brasilia, L4 Norte, CEP 70910-900, Brasilia-DF, Brazil.
authors.forename: Eduardo M M
authors.initials: EM
authors.lastname: Lima
authors.affiliation: University College of London, Institute of Orhopaedics and Musculoskeletal Science, Royal National Orthopaedics Hospital, Brockley Hill, HA4 7LP, Stanmore, United Kingdom.
authors.forename: Allen E
authors.initials: AE
authors.lastname: Goodship
mesh_terms: D050156:Adipogenesis / Q000276:immunology; D000273:Adipose Tissue / Q000166:cytology / Q000276:immunology*; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000276:immunology*; D039481:CD11b Antigen / Q000032:analysis; D020219:Chondrogenesis / Q000276:immunology; D005260:Female; D005434:Flow Cytometry / Q000662:veterinary; D015395:Histocompatibility Antigens Class I / Q000032:analysis; D018960:Hyaluronan Receptors / Q000032:analysis; D016130:Immunophenotyping / Q000662:veterinary*; D017493:Leukocyte Common Antigens / Q000032:analysis; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000276:immunology*; D010012:Osteogenesis / Q000276:immunology; D010641:Phenotype; D012756:Sheep / Q000276:immunology*; D013583:Synovial Membrane / Q000166:cytology / Q000276:immunology*
chemical_list: D039481:CD11b Antigen; D015395:Histocompatibility Antigens Class I; D018960:Hyaluronan Receptors; D017493:Leukocyte Common Antigens
keywords: Flow cytometry; MSC; Ovine; Surface markers