title: Monitoring the efficacy of dendritic cell vaccination by early detection of (99m) Tc-HMPAO-labelled CD4(+) T cells.
abstract: DC vaccines have been used to induce tumour-specific cytotoxic T cells . However, this approach to cancer immunotherapy has had limited success. To be successful, injected DCs need to migrate to the LNs where they can stimulate effector T cells . We and others have previously demonstrated by MRI that tumour antigen-pulsed-DCs labelled ex vivo with superparamagnetic iron oxide nanoparticles migrated to the draining LNs and are capable of activating antigen-specific T cells . The results from our study demonstrated that ex vivo superparamagnetic iron oxide nanoparticles-labelled and OVA-pulsed DCs prime cytotoxic CD8(+) T-cell responses to protect against a B16-OVA tumour challenge. In the clinic, a possible noninvasive surrogate marker for efficacy of DC vaccination is to image the specific migration and accumulation of T cells following DC vaccination.
authors.affiliation: Medical Research Council (MRC) Centre for Transplantation, King's College London, King's Health Partners, Guy's Hospital, London, United Kingdom; Division of Imaging Sciences and Biomedical Engineering, King's College London, St. Thomas Hospital, London, United Kingdom.
authors.forename: Ehsan
authors.initials: E
authors.lastname: Sharif-Paghaleh
authors.forename: John
authors.initials: J
authors.lastname: Leech
authors.forename: Kavitha
authors.initials: K
authors.lastname: Sunassee
authors.forename: Niwa
authors.initials: N
authors.lastname: Ali
authors.forename: Pervinder
authors.initials: P
authors.lastname: Sagoo
authors.forename: Robert I
authors.initials: RI
authors.lastname: Lechler
authors.forename: Lesley A
authors.initials: LA
authors.lastname: Smyth
authors.forename: Giovanna
authors.initials: G
authors.lastname: Lombardi
authors.forename: Gregory E
authors.initials: GE
authors.lastname: Mullen
mesh_terms: D000818:Animals; D003713:Dendritic Cells / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D019690:Technetium Tc 99m Exametazime; D015899:Tomography, Emission-Computed, Single-Photon; D014057:Tomography, X-Ray Computed; D014611:Vaccination
chemical_list: D019690:Technetium Tc 99m Exametazime
keywords: 99mTc-HMPAO; Adoptive transfer therapy; Non-invasive imaging; SPECT/CT; T-cell imaging