title: Overexpression of allene oxide cyclase improves the biosynthesis of artemisinin in Artemisia annua L.
abstract: Jasmonates (JAs) are important signaling molecules in plants and play crucial roles in stress responses, secondary metabolites' regulation, plant growth and development. In this study, the promoter of AaAOC, which was the key gene of jasmonate biosynthetic pathway, had been cloned. GUS staining showed that AaAOC was expressed ubiquitiously in A. annua. AaAOC gene was overexpressed under control of 35S promoter. RT-Q-PCR showed that the expression levels of AaAOC were increased from 1.6- to 5.2-fold in AaAOC-overexpression transgenic A. annua. The results of GC-MS showed that the content of endogenous jasmonic acid (JA) was 2- to 4.7-fold of the control level in AaAOC-overexpression plants. HPLC showed that the contents of artemisinin, dihydroartemisinic acid and artemisinic acid were increased significantly in AaAOC-overexpression plants. RT-Q-PCR showed that the expression levels of FPS (farnesyl diphosphate synthase), CYP71AV1 (cytochrome P450 dependent hydroxylase) and DBR2 (double bond reductase 2) were increased significantly in AaAOC-overexpression plants. All data demonstrated that increased endogenous JA could significantly promote the biosynthesis of artemisinin in AaAOC-overexpression transgenic A. annua.
authors.affiliation: Plant Biotechnology Research Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China; State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Lu
authors.affiliation: Plant Biotechnology Research Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Fangyuan
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Plant Biotechnology Research Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Shen
authors.affiliation: Plant Biotechnology Research Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Weimin
authors.initials: W
authors.lastname: Jiang
authors.affiliation: Plant Biotechnology Research Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Qifang
authors.initials: Q
authors.lastname: Pan
authors.affiliation: Plant Biotechnology Research Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Zongyou
authors.initials: Z
authors.lastname: Lv
authors.affiliation: Plant Biotechnology Research Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Tingxiang
authors.initials: T
authors.lastname: Yan
authors.affiliation: Plant Biotechnology Research Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Xueqing
authors.initials: X
authors.lastname: Fu
authors.affiliation: Plant Biotechnology Research Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Yuliang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Plant Biotechnology Research Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Qian
authors.affiliation: Plant Biotechnology Research Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Kexuan
authors.initials: K
authors.lastname: Tang
mesh_terms: D031172:Artemisia annua / Q000235:genetics* / Q000378:metabolism; D037621:Artemisinins / Q000378:metabolism*; D001483:Base Sequence; D003517:Cyclopentanes / Q000378:metabolism*; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D005818:Genetic Engineering; D051228:Geranyltranstransferase / Q000235:genetics / Q000378:metabolism; D019746:Intramolecular Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism; D054883:Oxylipins / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified
chemical_list: D037621:Artemisinins; D003517:Cyclopentanes; D054883:Oxylipins; D010940:Plant Proteins; C520462:dihydroartemisinic acid; C047721:artemisic acid; C011006:jasmonic acid; D003577:Cytochrome P-450 Enzyme System; C031327:artemisinin; D010088:Oxidoreductases; C531287:artemisinic aldehyde delta11(13) reductase, Artemisia annua; D051228:Geranyltranstransferase; D019746:Intramolecular Oxidoreductases; C021377:hydroperoxide isomerase