title: Lipase inhibition and antiobesity effect of Atractylodes lancea.
abstract: The ethanol extract of Atractylodes lancea rhizome displayed significant lipase inhibition with an IC50 value of 9.06 µg/mL in a human pancreatic lipase assay from high-throughput screening. Bioassay-guided isolation led to the identification of one new polyacetylene, syn-(5E,11E)-3-acetoxy-4-O-(3-methylbutanoyl)-1,5,11-tridecatriene-7,9-diyne-3,4-diol (7), along with six known compounds (1-6). The structure of compound 7 was determined based on the analysis of NMR and MS data. Among these seven lipase inhibitors, the major compound atractylodin (1) showed the highest lipase inhibitory activity (IC50 = 39.12 µM). The antiobesity effect of the ethanol extract of Atractylodes lancea rhizome was evaluated in a high-fat diet-induced obesity mice model at daily dosages of 250 mg/kg and 500 mg/kg body weight for 4 weeks, and treatment with this extract demonstrated a moderate efficacy at the 500 mg/kg dose level.
authors.affiliation: Unigen USA, Seattle, WA, USA.
authors.forename: Ping
authors.initials: P
authors.lastname: Jiao
authors.affiliation: Unigen USA, Seattle, WA, USA.
authors.forename: Julie
authors.initials: J
authors.lastname: Tseng-Crank
authors.affiliation: Unigen USA, Seattle, WA, USA.
authors.forename: Brandon
authors.initials: B
authors.lastname: Corneliusen
authors.affiliation: Unigen USA, Seattle, WA, USA.
authors.forename: Mesfin
authors.initials: M
authors.lastname: Yimam
authors.affiliation: Unigen USA, Seattle, WA, USA.
authors.forename: Mandee
authors.initials: M
authors.lastname: Hodges
authors.affiliation: Unigen USA, Seattle, WA, USA.
authors.forename: Mei
authors.initials: M
authors.lastname: Hong
authors.affiliation: Unigen USA, Seattle, WA, USA.
authors.forename: Catherine
authors.initials: C
authors.lastname: Maurseth
authors.affiliation: Unigen Korea, Cheonam Si, Chungnam, South Korea.
authors.forename: Misun
authors.initials: M
authors.lastname: Oh
authors.affiliation: Unigen Korea, Cheonam Si, Chungnam, South Korea.
authors.forename: Hyunjin
authors.initials: H
authors.lastname: Kim
authors.affiliation: Unigen USA, Seattle, WA, USA.
authors.forename: Min
authors.initials: M
authors.lastname: Chu
authors.affiliation: Unigen USA, Seattle, WA, USA.
authors.forename: Qi
authors.initials: Q
authors.lastname: Jia
mesh_terms: D000818:Animals; D019440:Anti-Obesity Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D031174:Atractylodes / Q000737:chemistry*; D001835:Body Weight; D002087:Butyrates / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D059305:Diet, High-Fat / Q000009:adverse effects; D004195:Disease Models, Animal; D005663:Furans / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D057166:High-Throughput Screening Assays; D006801:Humans; D020128:Inhibitory Concentration 50; D008049:Lipase / Q000037:antagonists & inhibitors*; D051379:Mice; D008810:Mice, Inbred C57BL; D015394:Molecular Structure; D010179:Pancreas / Q000201:enzymology; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D053279:Polyynes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D027343:Rhizome / Q000737:chemistry
chemical_list: D019440:Anti-Obesity Agents; D002087:Butyrates; D005663:Furans; D010936:Plant Extracts; D053279:Polyynes; C106914:atractylodin; D008049:Lipase