title: Comparison of Markers and Functional Attributes of Human Adipose-Derived Stem Cells and Dedifferentiated Adipocyte Cells from Subcutaneous Fat of an Obese Diabetic Donor.
abstract: Objective:Adipose tissue is a robust source of adipose-derived stem cells (ADSCs) that may be able to provide secreted factors that promote the ability of wounded tissue to heal. However, adipocytes also have the potential to dedifferentiate in culture to cells with stem cell-like properties that may improve their behavior and functionality for certain applications.Approach:ADSCs are adult mesenchymal stem cells that are cultured from the stromal vascular fraction of adipose tissue. However, adipocytes are capable of dedifferentiating into cells with stem cell properties. In this case study, we compare ADSC and dedifferentiated fat (DFAT) cells from the same patient and fat depot for mesenchymal cell markers, embryonic stem cell markers, ability to differentiate to adipocytes and osteoblasts, senescence and telomerase levels, and ability of conditioned media (CM) to stimulate migration of human dermal fibroblasts (HDFs).Innovation and Conclusions:ADSCs and DFAT cells displayed identical levels of CD90, CD44, CD105, and were CD34- and CD45-negative. They also expressed similar levels of Oct4, BMI1, KLF4, and SALL4. DFAT cells, however, showed higher efficiency in adipogenic and osteogenic capacity. Telomerase levels of DFAT cells were double those of ADSCs, and senescence declined in DFAT cells. CM from both cell types altered the migration of fibroblasts. Despite reports of ADSCs from a number of human depots, there have been no comparisons of the ability of dedifferentiated DFAT cells from the same donor and depot to differentiate or modulate migration of HDFs. Since ADSCs were from an obese diabetic donor, reprogramming of DFAT cells may help improve a patient's cells for regenerative medicine applications.
authors.affiliation: Research Service, James A. Haley Veterans Hospital , Tampa, Florida.
authors.forename: James E
authors.initials: JE
authors.lastname: Watson
authors.affiliation: Research Service, James A. Haley Veterans Hospital , Tampa, Florida. ; Department of Molecular Medicine, Morsani College of Medicine, University of South Florida , Tampa, Florida.
authors.forename: Niketa A
authors.initials: NA
authors.lastname: Patel
authors.affiliation: Research Service, James A. Haley Veterans Hospital , Tampa, Florida.
authors.forename: Gay
authors.initials: G
authors.lastname: Carter
authors.affiliation: Department of Surgery, Morsani College of Medicine, University of South Florida , Tampa, Florida.
authors.forename: Andrea
authors.initials: A
authors.lastname: Moor
authors.affiliation: Department of Molecular Medicine, Morsani College of Medicine, University of South Florida , Tampa, Florida.
authors.forename: Rekha
authors.initials: R
authors.lastname: Patel
authors.affiliation: Department of Molecular Medicine, Morsani College of Medicine, University of South Florida , Tampa, Florida.
authors.forename: Tomar
authors.initials: T
authors.lastname: Ghansah
authors.affiliation: Department of Surgery, Morsani College of Medicine, University of South Florida , Tampa, Florida.
authors.forename: Abhishek
authors.initials: A
authors.lastname: Mathur
authors.affiliation: Department of Surgery, Morsani College of Medicine, University of South Florida , Tampa, Florida.
authors.forename: Michel M
authors.initials: MM
authors.lastname: Murr
authors.affiliation: Research Service, James A. Haley Veterans Hospital , Tampa, Florida. ; Department of Neurosurgery, Morsani College of Medicine, University of South Florida , Tampa, Florida.
authors.forename: Paula
authors.initials: P
authors.lastname: Bickford
authors.affiliation: Research Service, James A. Haley Veterans Hospital , Tampa, Florida. ; Department of Surgery, Morsani College of Medicine, University of South Florida , Tampa, Florida.
authors.forename: Lisa J
authors.initials: LJ
authors.lastname: Gould
authors.affiliation: Research Service, James A. Haley Veterans Hospital , Tampa, Florida. ; Department of Molecular Medicine, Morsani College of Medicine, University of South Florida , Tampa, Florida.
authors.forename: Denise R
authors.initials: DR
authors.lastname: Cooper