title: Expression analysis of Dact1 in mice using a LacZ reporter.
abstract: The Wnt signaling pathway is essential for cell fate decisions during embryonic development as well as for homeostasis after birth. Dapper antagonist of catenin-1 (Dact1) plays an important role during embryogenesis by regulating Wnt signaling pathways. Consequently, targeted disruption of the Dact1 gene in mice leads to perinatal lethality due to severe developmental defects involving the central nervous system, genitourinary system and distal digestive tract. However, the expression and potential function of Dact1 in other tissues during development and postnatal life have not been well studied. Here, we have generated reporter mice in which LacZ expression is driven by the Dact1 gene promoter and characterized Dact1-LacZ expression in embryos and adult tissues. Our data show that while Dact1-LacZ is expressed in multiple mesoderm- and neuroectoderm-derived tissues during development, high expression of Dact1-LacZ is restricted to a small subset of adult tissues, including the brain, eye, heart, and some reproductive organs. These results will serve as a basis for future investigation of Dact1 function in Wnt-mediated organogenesis and tissue homeostasis.
authors.affiliation: Department of Animal Biology, University of Pennsylvania School of Veterinary Medicine, Philadelphia, PA 19104, USA; Institute for Regenerative Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Suzuki
authors.affiliation: Department of Animal Biology, University of Pennsylvania School of Veterinary Medicine, Philadelphia, PA 19104, USA.
authors.forename: N Adrian
authors.initials: NA
authors.lastname: Leu
authors.affiliation: University Laboratory Animal Resources, University of Pennsylvania School of Veterinary Medicine, Philadelphia, PA 19104, USA.
authors.forename: Angela K
authors.initials: AK
authors.lastname: Brice
authors.affiliation: Department of Animal Biology, University of Pennsylvania School of Veterinary Medicine, Philadelphia, PA 19104, USA; Institute for Regenerative Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA. Electronic address: msenoo@vet.upenn.edu.
authors.forename: Makoto
authors.initials: M
authors.lastname: Senoo
mesh_terms: D000818:Animals; D004622:Embryo, Mammalian / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics* / Q000378:metabolism*; D007763:Lac Operon; D008297:Male; D051379:Mice; D009928:Organ Specificity; D011401:Promoter Regions, Genetic; D016601:RNA-Binding Proteins
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; D016601:RNA-Binding Proteins; C508545:frodo protein, mouse
keywords: Dact1; Embryogenesis; Homeostasis; LacZ reporter; Wnt signal