title: Human lutropin (hLH) and choriogonadotropin (CG) are assembled by different pathways: a model of hLH assembly.
abstract: The glycoprotein hormones are all structurally related heterodimers consisting of an α-subunit and a ligand-specific β-subunit that confers their unique biological activity. Crystal structures showed how the β-subunit surrounds a part of the α-subunit, and we showed the existence of the two mechanisms responsible for that assembly. In human choriogonadotropin, the β-subunit is folded before the subunits dock, and the α-subunit becomes incorporated into the dimer by a mechanism we termed "threading," passing between parts of the preassembled β-subunit. Here, we show that the human lutropin β-subunit is not folded completely prior to its interaction with the α-subunit and show that docking of the subunits enables the α-subunit to serve as a chaperone to the β-subunit. Based on data described here, we propose that the α-subunit facilitates formation of the human lutropin β-subunit by two mechanisms. First, the cystine knot of the α-subunit potentiates formation of the β-subunit cystine knot, and second, contacts between α-subunit loop 2 and a hydrophobic tail in the β-subunit facilitate formation of the seatbelt latch disulfide, which stabilizes the heterodimer. The primary influence of the α-subunit was seen when the hydrophobic tail was present or absent, but the secondary mechanism was required only when the hydrophobic tail of the β-subunit was present. During the evolution of human choriogonadotropin, neither of these α-subunit roles was necessary for folding of the β-subunit. The complex mechanism for lutropin assembly may be required to provide an additional control on its positive feedback function in vertebrate reproduction.
authors.affiliation: From the Department of Obstetrics, Gynecology, and Reproductive Sciences.
authors.forename: Michael P
authors.initials: MP
authors.lastname: Bernard
authors.affiliation: From the Department of Obstetrics, Gynecology, and Reproductive Sciences.
authors.forename: Win
authors.initials: W
authors.lastname: Lin
authors.affiliation: the Office of Information Technology (OIT)/High Performance and Research Computing, and the Department of Pharmacology, Robert Wood Johnson Medical School, Rutgers Biomedical and Health Sciences, Piscataway, New Jersey 08854.
authors.forename: Vladyslav
authors.initials: V
authors.lastname: Kholodovych
authors.affiliation: From the Department of Obstetrics, Gynecology, and Reproductive Sciences, moyle@rwjms.rutgers.edu.
authors.forename: William R
authors.initials: WR
authors.lastname: Moyle
mesh_terms: D006063:Chorionic Gonadotropin / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D007986:Luteinizing Hormone / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D055503:Protein Multimerization; D020836:Protein Structure, Quaternary; D021122:Protein Subunits / Q000737:chemistry / Q000378:metabolism
chemical_list: D006063:Chorionic Gonadotropin; D021122:Protein Subunits; D007986:Luteinizing Hormone
keywords: Glycoprotein Hormones; Human Choriogonadotropin; Human Follitropin; Human Lutropin; Human Thyrotropin; Mutagenesis; Protein Chimeras; Protein Cross-linking; Protein Folding