title: Testicular membrane lipid damage by complex mixture of leachate from municipal battery recycling site as indication of idiopathic male infertility in rat.
abstract: Leachate from a municipal battery recycling site is a potent source of mixed-metal released into the environment. The present study investigated the degree at which mixed-metal exposure to the municipal auto-battery leachate (MABL) and to the Elewi Odo municipal auto-battery recycling site leachate (EOMABRL) affected the lipid membrane of the testes in in vitro experiment. The results showed elevated level of mixed-metals over the permissible levels in drinking water, as recommended by regulatory authorities. In the leachate samples, the levels of malondialdehyde (MDA), a biomarker of lipid damage, was significantly (p<0.05) increased in rat testes in a dose-dependent manner. MDA induced by the municipal auto-battery leachate (MABL) was significantly (p<0.05) higher than the leachate from Elewi Odo municipal auto-battery recycling site (EOMABRL). The testicular lipid membrane capacity was compromised following treatment with leachate from the municipal battery recycling site, implicating mixed-metal exposure as the causative agent of testicular damage and male infertility.
authors.affiliation: Functional Foods, Nutraceuticals and Phytomedicine Research Laboratory, Department of Biochemistry, Federal University of Technology, Akure, Nigeria ; Department of Biosciences and Biotechnology, Biochemistry unit, College of Pure and Applied Sciences, Kwara State University, Malete, Nigeria.
authors.forename: Jacob K
authors.initials: JK
authors.lastname: Akintunde
authors.affiliation: Functional Foods, Nutraceuticals and Phytomedicine Research Laboratory, Department of Biochemistry, Federal University of Technology, Akure, Nigeria.
authors.forename: Ganiyu
authors.initials: G
authors.lastname: Oboh
authors.affiliation: Functional Foods, Nutraceuticals and Phytomedicine Research Laboratory, Department of Biochemistry, Federal University of Technology, Akure, Nigeria.
authors.forename: Akintunde A
authors.initials: AA
authors.lastname: Akindahunsi
keywords: EOMABRL; infertility; lipid membrane; mixed-metal exposure; rat