title: Sequential acquisition of multi-dimensional heteronuclear chemical shift correlation spectra with ¹H detection.
abstract: RF pulse schemes for the simultaneous acquisition of heteronuclear multi-dimensional chemical shift correlation spectra, such as {HA(CA)NH & HA(CACO)NH}, {HA(CA)NH & H(N)CAHA} and {H(N)CAHA & H(CC)NH}, that are commonly employed in the study of moderately-sized protein molecules, have been implemented using dual sequential (1)H acquisitions in the direct dimension. Such an approach is not only beneficial in terms of the reduction of experimental time as compared to data collection via two separate experiments but also facilitates the unambiguous sequential linking of the backbone amino acid residues. The potential of sequential (1)H data acquisition procedure in the study of RNA is also demonstrated here.
authors.affiliation: Leibniz Institute for Age Research - Fritz Lipmann Institute, Department Biomolecular NMR Spectroscopy, Beutenbergstraße 11, 07745 Jena, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Bellstedt
authors.affiliation: Leibniz Institute for Age Research - Fritz Lipmann Institute, Department Biomolecular NMR Spectroscopy, Beutenbergstraße 11, 07745 Jena, Germany.
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Ihle
authors.affiliation: Leibniz Institute for Age Research - Fritz Lipmann Institute, Department Biomolecular NMR Spectroscopy, Beutenbergstraße 11, 07745 Jena, Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Wiedemann
authors.affiliation: 1] Leibniz Institute for Age Research - Fritz Lipmann Institute, Department Biomolecular NMR Spectroscopy, Beutenbergstraße 11, 07745 Jena, Germany [2].
authors.forename: Anika
authors.initials: A
authors.lastname: Kirschstein
authors.affiliation: Ubon Ratchathani University, Department of Physics, 34190 Ubon Ratchathani, Thailand.
authors.forename: Christian
authors.initials: C
authors.lastname: Herbst
authors.affiliation: Leibniz Institute for Age Research - Fritz Lipmann Institute, Department Biomolecular NMR Spectroscopy, Beutenbergstraße 11, 07745 Jena, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Görlach
authors.affiliation: Leibniz Institute for Age Research - Fritz Lipmann Institute, Department Biomolecular NMR Spectroscopy, Beutenbergstraße 11, 07745 Jena, Germany.
authors.forename: Ramadurai
authors.initials: R
authors.lastname: Ramachandran