title: Molecular detection of genogroup I and II picobirnaviruses in pigs in China.
abstract: In this study, a total of 187 stool specimens were collected from a pig farm in Hunan province of China, from November 2011 to June 2012. 39 (20.9 %) stool specimens were positive for picobirnaviruses using reverse transcription-polymerase chain reaction. Among 39 stool specimens, 84.6 % (33/39) were identified to be genogroup I (prototype 1-CHN-97), 38.5 % (15/39) belonged to genogroup II (prototype 4-GA-91), and 23.1 % (9/39) of which showed the evidence of genogroup I picobirnavirus were also positive for genogroup II picobirnaviruses. Picobirnaviruses exist in pigs which were divided into five groups according to the age and physiological status. Nineteen representative strains of genogroup I picobirnaviruses and eleven strains of genogroup II picobirnaviruses detected in this study were selected to analyze their phylogenetic relationships with other picobirnaviruses reference strains. The phylogenetic tree analysis suggested the prevalence of multiple picobirnaviruses in pigs in China.
authors.affiliation: Shanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China.
authors.forename: Molin
authors.initials: M
authors.lastname: Chen
authors.forename: Huan
authors.initials: H
authors.lastname: Sun
authors.forename: Daoliang
authors.initials: D
authors.lastname: Lan
authors.forename: Xiuguo
authors.initials: X
authors.lastname: Hua
authors.forename: Li
authors.initials: L
authors.lastname: Cui
authors.forename: Congli
authors.initials: C
authors.lastname: Yuan
authors.forename: Zhibiao
authors.initials: Z
authors.lastname: Yang
mesh_terms: D000818:Animals; D002681:China; D005243:Feces / Q000821:virology; D008969:Molecular Sequence Data; D010802:Phylogeny; D018708:Picobirnavirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D012327:RNA Virus Infections / Q000662:veterinary* / Q000821:virology; D013552:Swine; D013553:Swine Diseases / Q000821:virology*