title: Characteristics and biotechnology applications of aliphatic amino acid hydroxylases belonging to the Fe(II)/α-ketoglutarate-dependent dioxygenase superfamily.
abstract: The asymmetric hydroxylation of inactive carbon atoms is still an important reaction in the industrial synthesis of valuable chiral compounds such as pharmaceuticals and fine chemicals. Applications of monooxygenation enzymes, like cytochrome P450 monooxygenases, flavin-containing monooxygenases, and Fe(II)/α-ketoglutarate-dependent dioxygenases (Fe/αKG-DOs), are strongly desired as hydroxylation biocatalysts because they have great advantages in regio- and stereoselectivity of the reactions. Recently, several novel Fe/αKG-DOs have been found to catalyze the asymmetric hydroxylation of aliphatic amino acids. Depending on their amino acid sequences, these Fe/αKG-DOs catalyze different types of regioselective hydroxylations, or C3-, C4-, and C5-hydroxylation. Additionally, most also have stereoselective sulfoxidation activities. Here, we have reviewed the characterization and process development of this novel functioning group of Fe/αKG-DOs.
authors.affiliation: Industrial Microbiology, Graduate School of Agriculture, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto, 606-8502, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Hibi
authors.forename: Jun
authors.initials: J
authors.lastname: Ogawa
mesh_terms: D000596:Amino Acids / Q000378:metabolism*; D001709:Biotechnology / Q000379:methods*; D049308:Dioxygenases / Q000378:metabolism*; D007501:Iron / Q000378:metabolism; D007656:Ketoglutaric Acids / Q000378:metabolism; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D013678:Technology, Pharmaceutical / Q000379:methods
chemical_list: D000596:Amino Acids; D007656:Ketoglutaric Acids; D007501:Iron; D006899:Mixed Function Oxygenases; D049308:Dioxygenases