title: Hepatoprotective bioactivity of the glycoprotein, antrodan, isolated from Antrodia cinnamomea mycelia.
abstract: Antrodan, a protein-bound polysaccharide isolated from Antrodia cinnamomea mycelia, was demonstrated to exhibit significant anti-inflammatory bioactivity in vitro. However, its role in hepatic injury in vivo still remains unclear. We hypothesized that antrodan may have beneficial hepatoprotective effects. To verify this, a lipopolysaccharide (LPS)-Sprague-Dawley rat model was used. Antrodan protected against liver damage by suppressing LPS-stimulated serum glutamine-oxaloacetic transaminase (GOT), glutamic-pyruvic transaminase (GPT), interleukin (IL)-6, hepatic thiobarbituric acid reactive substances (TBARS), nitric oxide (NO), inducible NO synthase (iNOS) and nuclear factor (NF)-κB, and by effectively alleviating the downregulated hepatic superoxide dismutase (SOD), catalase, and glutathione peroxidase (GSH-Px). Hematoxylin-eosin staining revealed that antrodan at a dosage of 40 mg/kg was able to alleviate LPS-induced liver damage to a normal status. In addition, we identified the partial main architectural backbone of antrodan to have a 1 → 3 linear β-glycosidic backbone of mannan linked by β-1 → 3 glucosidic branches. Conclusively, antrodan can potentially ameliorate liver damage in vivo by suppressing oxidative stress induced by LPS.
authors.affiliation: Department of Applied Food Technology, Hungkuang University, Taichung, Taiwan, ROC.
authors.forename: Yaw-Bee
authors.initials: YB
authors.lastname: Ker
authors.affiliation: Graduate Institute of Clinical Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan, ROC.
authors.forename: Chiung-Chi
authors.initials: CC
authors.lastname: Peng
authors.affiliation: Research Institute of Biotechnology, Hungkuang University, Taichung, Taiwan, ROC.
authors.forename: Wan-Lin
authors.initials: WL
authors.lastname: Chang
authors.affiliation: Research Institute of Biotechnology, Hungkuang University, Taichung, Taiwan, ROC.
authors.forename: Charng-Cherng
authors.initials: CC
authors.lastname: Chyau
authors.affiliation: Research Institute of Biotechnology, Hungkuang University, Taichung, Taiwan, ROC.
authors.forename: Robert Y
authors.initials: RY
authors.lastname: Peng
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D055491:Antrodia / Q000378:metabolism*; D005656:Fungal Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006023:Glycoproteins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D015850:Interleukin-6 / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000378:metabolism*; D008111:Liver Function Tests; D008297:Male; D025282:Mycelium; D016328:NF-kappa B / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D020011:Protective Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D000975:Antioxidants; D005656:Fungal Proteins; D006023:Glycoproteins; D015850:Interleukin-6; D016328:NF-kappa B; D020011:Protective Agents; D017392:Thiobarbituric Acid Reactive Substances; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II