title: Development of a sequential multicolor-FISH approach with 13 chromosome-specific painting probes for the rapid identification of river buffalo (Bubalus bubalis, 2n = 50) chromosomes.
abstract: The development of new molecular techniques (array CGH, M-FISH, SKY-FISH, etc.) has led to great advancements in the entire field of molecular cytogenetics. However, the application of these methods is still very limited in farm animals. In the present study, we report, for the first time, the production of 13 river buffalo (Bubalus bubalis, 2n = 50) chromosome-specific painting probes, generated via chromosome microdissection and the DOP-PCR procedure. A sequential multicolor-FISH approach is also proposed on the same slide for the rapid identification of river buffalo chromosome/arms, namely, 1p-1q, 2p-2q, 3p-3q, 4p-4q, 5p-5q, 18, X, and Y, using both conventional and late-replicating banded chromosome preparations counterstained by DAPI. The provided 'bank' of chromosome-specific painting probes is useful for any further cytogenetic investigation not only for the buffalo breeds, but also for other species of the family Bovidae, such as cattle, sheep, and goats, for chromosome abnormality diagnosis, and, more generally, for evolutionary studies.
authors.affiliation: ISPAAM, Laboratory of Animal Cytogenetics and Gene Mapping, National Research Council (CNR), via Argine 1085, 80147, Naples, Italy, alfredo.pauciullo@cnr.it.
authors.forename: Alfredo
authors.initials: A
authors.lastname: Pauciullo
authors.forename: Angela
authors.initials: A
authors.lastname: Perucatti
authors.forename: Alessandra
authors.initials: A
authors.lastname: Iannuzzi
authors.forename: Domenico
authors.initials: D
authors.lastname: Incarnato
authors.forename: Viviana
authors.initials: V
authors.lastname: Genualdo
authors.forename: Dino
authors.initials: D
authors.lastname: Di Berardino
authors.forename: Leopoldo
authors.initials: L
authors.lastname: Iannuzzi
mesh_terms: D000818:Animals; D002020:Buffaloes / Q000145:classification* / Q000235:genetics*; D002417:Cattle; D002871:Chromosome Banding; D002875:Chromosomes / Q000145:classification / Q000235:genetics*; D015342:DNA Probes; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007621:Karyotyping; D013045:Species Specificity
chemical_list: D015342:DNA Probes