title: Screening of plant growth-promoting traits in arsenic-resistant bacteria isolated from agricultural soil and their potential implication for arsenic bioremediation.
abstract: Twelve arsenic (As)-resistant bacteria (minimum inhibitory concentration ranging from 10 to 30mM and 150 to 320mM for As(III) and As(V), respectively) were isolated from the agricultural soil of the Chianan Plain in southwestern Taiwan using enrichment techniques. Eight isolates capable of oxidizing As(III) (rate of oxidation from 0.029 to 0.059μMh(-1) 10(-9) cell) and exhibiting As(III)-oxidase enzyme activity belong to Pseudomonas, Acinetobacter, Klebsiella and Comamonas genera, whereas four isolates that did not show As(III)-oxidizing activity belong to Geobacillus, Bacillus, Paenibacillus, and Enterobacter genera. Assessment of the parameters of plant growth promotion revealed that Pseudomonas sp. ASR1, ASR2 and ASR3, Geobacillus sp. ASR4, Bacillus sp. ASR5, Paenibacillus sp. ASR6, Enterobacter sp. ASR10 and Comamonas sp. ASR11, and ASR12 possessed some or all of the studied plant growth-promoting traits, including phosphate-solubilization, siderophore, IAA-like molecules and ACC deaminase production. In addition, the ability of As-resistant isolates to grow over wide ranges of pH and temperatures signify their potential application for sustainable bioremediation of As in the environment.
authors.affiliation: Department of Earth Sciences, National Cheng Kung University, Tainan, Taiwan.
authors.forename: Suvendu
authors.initials: S
authors.lastname: Das
authors.affiliation: Department of Earth Sciences, National Cheng Kung University, Tainan, Taiwan. Electronic address: jiinshuh@mail.ncku.edu.tw.
authors.forename: Jiin-Shuh
authors.initials: JS
authors.lastname: Jean
authors.affiliation: Department of Earth Sciences, National Cheng Kung University, Tainan, Taiwan.
authors.forename: Sandeep
authors.initials: S
authors.lastname: Kar
authors.affiliation: Department of Earth Sciences, National Cheng Kung University, Tainan, Taiwan.
authors.forename: Mon-Lin
authors.initials: ML
authors.lastname: Chou
authors.affiliation: Department of Earth and Environmental Sciences, National Chung Cheng University, Chiayi County, Taiwan.
authors.forename: Chien-Yen
authors.initials: CY
authors.lastname: Chen
mesh_terms: D001151:Arsenic / Q000737:chemistry*; D001419:Bacteria / Q000302:isolation & purification*; D001673:Biodegradation, Environmental; D006863:Hydrogen-Ion Concentration; D008826:Microbial Sensitivity Tests; D010100:Oxygen / Q000737:chemistry; D010710:Phosphates / Q000737:chemistry; D010802:Phylogeny; D010944:Plants / Q000187:drug effects / Q000382:microbiology*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000737:chemistry*; D013696:Temperature
chemical_list: D010710:Phosphates; D012989:Soil Pollutants; D001151:Arsenic; D010100:Oxygen
keywords: Agricultural soil; Arsenite oxidation; Bioremediation; Chemolithotrophs; Plant growth promotion