title: Expression of GAD67 and Dlx5 in the taste buds of mice genetically lacking Mash1.
abstract: It has been reported that a subset of type III taste cells express glutamate decarboxylase (GAD)67, which is a molecule that synthesizes gamma-aminobutyric acid (GABA), and that Mash1 could be a potential regulator of the development of GABAnergic neurons via Dlx transcription factors in the central nervous system. In this study, we investigated the expression of GAD67 and Dlx in the embryonic taste buds of the soft palate and circumvallate papilla using Mash1 knockout (KO)/GAD67-GFP knock-in mice. In the wild-type animal, a subset of type III taste cells contained GAD67 in the taste buds of the soft palate and the developing circumvallate papilla, whereas GAD67-expressing taste bud cells were missing from Mash1 KO mice. A subset of type III cells expressed mRNA for Dlx5 in the wild-type animals, whereas Dlx5-expressing cells were not evident in the apical part of the circumvallate papilla and taste buds in the soft palate of Mash1 KO mice. Our results suggest that Mash1 is required for the expression of GAD67 and Dlx5 in taste bud cells.
authors.affiliation: Division of Oral Histology and Neurobiology, Kyushu Dental University, 2-6-1 Manaduru, Kokurakita-ku, Kitakyushu 803-8580, Japan, Division of Special Needs and Geriatric Dentistry, Kyushu Dental University, 2-6-1 Manaduru, Kokurakita-ku, Kitakyushu 803-8580, Japan.
authors.forename: Ayae
authors.initials: A
authors.lastname: Kito-Shingaki
authors.affiliation: Division of Oral Histology and Neurobiology, Kyushu Dental University, 2-6-1 Manaduru, Kokurakita-ku, Kitakyushu 803-8580, Japan, yujiseta@kyu-dent.ac.jp.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Seta
authors.affiliation: Division of Oral Histology and Neurobiology, Kyushu Dental University, 2-6-1 Manaduru, Kokurakita-ku, Kitakyushu 803-8580, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Toyono
authors.affiliation: Division of Oral Anatomy, Kyushu Dental University, 2-6-1 Manaduru, Kokurakita-ku, Kitakyushu 803-8580, Japan and.
authors.forename: Shinji
authors.initials: S
authors.lastname: Kataoka
authors.affiliation: Division of Special Needs and Geriatric Dentistry, Kyushu Dental University, 2-6-1 Manaduru, Kokurakita-ku, Kitakyushu 803-8580, Japan.
authors.forename: Yasuaki
authors.initials: Y
authors.lastname: Kakinoki
authors.affiliation: Department of Genetic and Behavioral Neuroscience, Gunma University Graduate School of Medicine, 3-39-22 Showa-machi, Maebashi 371-8511, Japan.
authors.forename: Yuchio
authors.initials: Y
authors.lastname: Yanagawa
authors.affiliation: Division of Oral Histology and Neurobiology, Kyushu Dental University, 2-6-1 Manaduru, Kokurakita-ku, Kitakyushu 803-8580, Japan.
authors.forename: Kuniaki
authors.initials: K
authors.lastname: Toyoshima
mesh_terms: D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D005968:Glutamate Decarboxylase / Q000235:genetics / Q000378:metabolism*; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D018345:Mice, Knockout; D010160:Palate, Soft / Q000196:embryology / Q000378:metabolism; D013650:Taste Buds / Q000378:metabolism*; D014059:Tongue / Q000196:embryology / Q000378:metabolism
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D005968:Glutamate Decarboxylase; D018398:Homeodomain Proteins; C499317:Ascl1 protein, mouse; C501120:Dlx5 protein, mouse; C402565:glutamate decarboxylase 1
keywords: Dlx; Mash1; glutamate decarboxylase 67; taste bud; type III cell