title: [Simultaneous determination of clopidol, sulfonamide and quinolone residues in chickens by ultra performance liquid chromatography-tandem mass spectrometry].
abstract: An analytical method for the simultaneous determination of clopidol, sulfadiazine, sulfamethazine, sulfametoxydiazine, sulfamethoxypyridazine, norfloxacin, ofloxacin, ciprofloxacin, enrofloxacin in chickens by ultra performance liquid chromatography coupled with tandem quadrupole mass spectrometry (UPLC-MS/MS) in positive ion mode with multiple reaction monitoring (MRM) has been developed and validated. The samples were homogenized and extracted with acetonitrile. After defatted with high speed frozen centrifugation, the supernatant solution was evaporated and the residue was dissolved with the mobile phase and defatted with n-hexane. It was then analyzed with UPLC-MS/MS. The limit of detection of this method was 0.1 microg/kg, and the limit of quantification was 0.5 microg/kg. The average recoveries (spiked at the levels of 0.5, 1.0, 2.0 microg/kg) ranged from 81.5% to 97.6%, with the relative standard deviations between 2.1% and 8.9%. The results demonstrated that the method is simple, accurate and suitable for the identification and quantification of these drug residues in chickens.
authors.forename: Wei
authors.initials: W
authors.lastname: Song
authors.forename: Yanyun
authors.initials: Y
authors.lastname: Hu
authors.forename: Fang
authors.initials: F
authors.lastname: Han
authors.forename: Yaning
authors.initials: Y
authors.lastname: Lü
authors.forename: Ping
authors.initials: P
authors.lastname: Zheng
authors.forename: Fangfang
authors.initials: F
authors.lastname: Zhou
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Chen
authors.forename: Jing
authors.initials: J
authors.lastname: Qiu
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Zhou
mesh_terms: D000818:Animals; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D002939:Ciprofloxacin; D003007:Clopidol / Q000032:analysis*; D004350:Drug Residues / Q000032:analysis*; D000077422:Enrofloxacin; D024841:Fluoroquinolones; D005506:Food Contamination / Q000032:analysis*; D006586:Hexanes; D008460:Meat / Q000032:analysis*; D009643:Norfloxacin; D015242:Ofloxacin; D015363:Quinolones / Q000032:analysis*; D000077145:Sulfanilamide; D013424:Sulfanilamides; D013449:Sulfonamides / Q000032:analysis*; D053719:Tandem Mass Spectrometry
chemical_list: D024841:Fluoroquinolones; D006586:Hexanes; D015363:Quinolones; D013424:Sulfanilamides; D013449:Sulfonamides; D000077145:Sulfanilamide; C026385:n-hexane; D000077422:Enrofloxacin; D002939:Ciprofloxacin; D003007:Clopidol; D015242:Ofloxacin; D009643:Norfloxacin