title: Magnolia extract (BL153) protection of heart from lipid accumulation caused cardiac oxidative damage, inflammation, and cell death in high-fat diet fed mice.
abstract: Magnolia as an herbal material obtained from Magnolia officinalis has been found to play an important role in anti-inflammation, antioxidative stress, and antiapoptosis. This study was designed to investigate the effect of Magnolia extract (BL153) on obesity-associated lipid accumulation, inflammation, oxidative stress, and apoptosis in the heart. C57BL/6 mice were fed a low- (10 kcal% fat) or high-fat (60 kcal% fat) diet for 24 weeks to induce obesity. These mice fed with high-fat diet (HFD) were given a gavage of vehicle, 2.5, 5, or 10 mg/kg body weight BL153 daily. The three doses of BL153 treatment slightly ameliorated insulin resistance without decrease of body weight gain induced by HFD feeding. BL153 at 10 mg/kg slightly attenuated a mild cardiac hypertrophy and dysfunction induced by HFD feeding. Both 5 mg/kg and 10 mg/kg of BL153 treatment significantly inhibited cardiac lipid accumulation measured by Oil Red O staining and improved cardiac inflammation and oxidative stress by downregulating ICAM-1, TNF-α, PAI-1, 3-NT, and 4-HNE. TUNEL staining showed that BL153 treatment also ameliorated apoptosis induced by mitochondrial caspase-3 independent cell death pathway. This study demonstrates that BL153 attenuates HFD-associated cardiac damage through prevention of HFD-induced cardiac lipid accumulation, inflammation, oxidative stress, and apoptosis.
authors.affiliation: Departments of Nephrology and Cardiology, The First Hospital of Jilin University, Changchun 130021, China ; Kosair Children's Hospital Research Institute, Department of Pediatrics, University of Louisville, Louisville, KY 40202, USA.
authors.forename: Weixia
authors.initials: W
authors.lastname: Sun
authors.affiliation: Departments of Nephrology and Cardiology, The First Hospital of Jilin University, Changchun 130021, China ; Kosair Children's Hospital Research Institute, Department of Pediatrics, University of Louisville, Louisville, KY 40202, USA.
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Kosair Children's Hospital Research Institute, Department of Pediatrics, University of Louisville, Louisville, KY 40202, USA ; Preventive Medicine School, Jilin University, Changchun 130021, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Departments of Nephrology and Cardiology, The First Hospital of Jilin University, Changchun 130021, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Yin
authors.affiliation: Departments of Nephrology and Cardiology, The First Hospital of Jilin University, Changchun 130021, China.
authors.forename: Yaowen
authors.initials: Y
authors.lastname: Fu
authors.affiliation: Departments of Nephrology and Cardiology, The First Hospital of Jilin University, Changchun 130021, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Kosair Children's Hospital Research Institute, Department of Pediatrics, University of Louisville, Louisville, KY 40202, USA ; The Chinese-American Research Institute for Diabetic Complications, The Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Cai
authors.affiliation: Bioland Biotec Co., Ltd., Zhangjiang Modern Medical Device Park, Pudong, Shanghai 201201, China.
authors.forename: Ki-Soo
authors.initials: KS
authors.lastname: Kim
authors.affiliation: Bioland R&D Center, 59 Songjeongni 2-gil, Byeongcheon, Dongnam, Cheonan, Chungnam 330-863, Republic of Korea.
authors.forename: Ki Ho
authors.initials: KH
authors.lastname: Kim
authors.affiliation: Kosair Children's Hospital Research Institute, Department of Pediatrics, University of Louisville, Louisville, KY 40202, USA ; The Chinese-American Research Institute for Diabetic Complications, The Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Tan
authors.affiliation: Bioland R&D Center, 59 Songjeongni 2-gil, Byeongcheon, Dongnam, Cheonan, Chungnam 330-863, Republic of Korea.
authors.forename: Young Heui
authors.initials: YH
authors.lastname: Kim
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D002316:Cardiotonic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D016923:Cell Death / Q000187:drug effects; D059305:Diet, High-Fat / Q000009:adverse effects*; D059865:Endoplasmic Reticulum Stress / Q000187:drug effects; D002149:Energy Intake / Q000187:drug effects; D005247:Feeding Behavior / Q000187:drug effects; D006334:Heart Function Tests; D007249:Inflammation / Q000188:drug therapy / Q000473:pathology*; D007333:Insulin Resistance; D050356:Lipid Metabolism / Q000187:drug effects*; D031566:Magnolia / Q000737:chemistry; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism / Q000473:pathology*; D009929:Organ Size / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D015398:Signal Transduction / Q000187:drug effects; D014280:Triglycerides / Q000097:blood
chemical_list: D015415:Biomarkers; D002316:Cardiotonic Agents; D010936:Plant Extracts; D014280:Triglycerides