title: Expression and purification of soluble monomeric streptavidin in Escherichia coli.
abstract: We recently reported the engineering of monomeric streptavidin (mSA) for use in monomeric detection of biotinylated ligands. Although mSA can be expressed functionally on the surface of mammalian cells and yeast, the molecule does not fold correctly when expressed in Escherichia coli. Refolding from inclusion bodies is cumbersome and yields a limited amount of purified protein. Improving the final yield should facilitate its use in biotechnology. We tested the expression and purification of mSA fused to GST, MBP, thioredoxin, and sumo tags to simplify its purification and improve the yield. The fusion proteins can be expressed solubly in E. coli and increase the yield by more than 20-fold. Unmodified mSA can be obtained by proteolytically removing the fusion tag. Purified mSA can be immobilized on a solid matrix to purify biotinylated ligands. Together, expressing mSA as a fusion with a solubilization tag vastly simplifies its preparation and increases its usability in biotechnology.
authors.affiliation: Department of Chemical and Biological Engineering, University at Buffalo, Buffalo, NY, 14260, USA.
authors.forename: Daniel
authors.initials: D
authors.lastname: Demonte
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Dundas
authors.forename: Sheldon
authors.initials: S
authors.lastname: Park
mesh_terms: D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D012995:Solubility; D019809:Streptavidin / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics
chemical_list: D011993:Recombinant Fusion Proteins; D019809:Streptavidin