title: Pseudozyma brasiliensis sp. nov., a xylanolytic, ustilaginomycetous yeast species isolated from an insect pest of sugarcane roots.
abstract: A novel ustilaginomycetous yeast isolated from the intestinal tract of an insect pest of sugarcane roots in Ribeirão Preto, São Paulo State, Brazil, represents a novel species of the genus Pseudozyma based on molecular analyses of the D1/D2 rDNA large subunit and the internal transcribed spacer (ITS1+ITS2) regions. The name Pseudozyma brasiliensis sp. nov. is proposed for this species, with GHG001(T) ( = CBS 13268(T) = UFMG-CM-Y307(T)) as the type strain. P. brasiliensis sp. nov. is a sister species of Pseudozyma vetiver, originally isolated from leaves of vetiver grass and sugarcane in Thailand. P. brasiliensis sp. nov. is able to grow well with xylan as the sole carbon source and produces high levels of an endo-1,4-xylanase that has a higher specific activity in comparison with other eukaryotic xylanases. This enzyme has a variety of industrial applications, indicating the great biotechnological potential of P. brasiliensis.
authors.affiliation: Laboratório Nacional de Ciência e Tecnologia do Bioetanol (CTBE), Centro Nacional de Pesquisa em Energia e Materiais (CNPEM), Caixa Postal 6170, 13083-970 Campinas, São Paulo, Brazil.
authors.forename: Juliana Velasco de Castro
authors.initials: JVC
authors.lastname: Oliveira
authors.affiliation: Laboratório Nacional de Ciência e Tecnologia do Bioetanol (CTBE), Centro Nacional de Pesquisa em Energia e Materiais (CNPEM), Caixa Postal 6170, 13083-970 Campinas, São Paulo, Brazil.
authors.forename: Thuanny A
authors.initials: TA
authors.lastname: Borges
authors.affiliation: Laboratório Nacional de Ciência e Tecnologia do Bioetanol (CTBE), Centro Nacional de Pesquisa em Energia e Materiais (CNPEM), Caixa Postal 6170, 13083-970 Campinas, São Paulo, Brazil.
authors.forename: Renato Augusto
authors.initials: RA
authors.lastname: Corrêa Dos Santos
authors.affiliation: Departamento de Microbiologia, Universidade Federal de Minas Gerais, Instituto de Ciências Biológicas, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Larissa F D
authors.initials: LFD
authors.lastname: Freitas
authors.affiliation: Departamento de Microbiologia, Universidade Federal de Minas Gerais, Instituto de Ciências Biológicas, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Carlos Augusto
authors.initials: CA
authors.lastname: Rosa
authors.affiliation: Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, São Paulo, Brazil.|Laboratório Nacional de Ciência e Tecnologia do Bioetanol (CTBE), Centro Nacional de Pesquisa em Energia e Materiais (CNPEM), Caixa Postal 6170, 13083-970 Campinas, São Paulo, Brazil.
authors.forename: Gustavo Henrique
authors.initials: GH
authors.lastname: Goldman
authors.affiliation: Laboratório Nacional de Ciência e Tecnologia do Bioetanol (CTBE), Centro Nacional de Pesquisa em Energia e Materiais (CNPEM), Caixa Postal 6170, 13083-970 Campinas, São Paulo, Brazil.
authors.forename: Diego Mauricio
authors.initials: DM
authors.lastname: Riaño-Pachón
mesh_terms: D000818:Animals; D001938:Brazil; D004271:DNA, Fungal / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D043364:Endo-1,4-beta Xylanases / Q000378:metabolism; D007313:Insecta / Q000382:microbiology*; D007422:Intestines / Q000382:microbiology; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D010802:Phylogeny; D018517:Plant Roots; D031786:Saccharum; D017422:Sequence Analysis, DNA; D014587:Ustilaginales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D043364:Endo-1,4-beta Xylanases