title: Determination of organothiophosphorus pesticides in water by liquid chromatography and post-column chemiluminescence with cerium(IV).
abstract: A new, fast, selective and sensitive method has been developed for the simultaneous determination of nine organothiophosphorus (OTP) pesticides, namely omethoate, dimethoate, disulfoton-sulfoxide, methidathion, phosmet, malathion, diazinon, pirimiphos-methyl and chlorpyrifos. The pesticides were separated on a Kinetex C18 column by gradient elution with acetonitrile:water. A post-column basic hydrolysis of the pesticides and later a chemiluminescence (CL) reaction with cerium (IV) in acid medium was carried out. Hexadecylpyridinium chloride highly enhanced the CL emission. Under optimized conditions, linearity, precision, limits of detection and quantification, and accuracy were determined. Both selectivity and sensitivity were compared with those obtained with UV detection. In combination with SPE, limits of detection in the range 15-80ng/L and 5-30ng/L were obtained when 250mL and 1000mL of solution were treated, respectively. When applied to 250mL of sample the inter-day precision of the method was between 3.5% and 7.3% and the intra-day precision between 2.9% and 6.0%. The method was applied to determine OTP pesticides in spiked water samples from different origins: irrigation, river, sea, ground, spring, mineral and tap waters, being the percentage of recovery of added amounts near 100% form most of the pesticides.
authors.affiliation: Instituto de Investigación para la Gestión Integrada de Zonas Costeras, Universidad Politécnica de Valencia, C/Paranimf no.1, Grao de Gandía, 46730 Valencia, Spain. Electronic address: mocaic@qim.upv.es.
authors.forename: Mónica
authors.initials: M
authors.lastname: Catalá-Icardo
authors.affiliation: Departamento de Farmacia, Universidad CEU-Cardenal Herrera, Avenida Seminario s/n, Moncada, 46113 Valencia, Spain.
authors.forename: Luis
authors.initials: L
authors.lastname: Lahuerta-Zamora
authors.affiliation: Instituto de Investigación para la Gestión Integrada de Zonas Costeras, Universidad Politécnica de Valencia, C/Paranimf no.1, Grao de Gandía, 46730 Valencia, Spain; Departamento de Farmacia, Universidad CEU-Cardenal Herrera, Avenida Seminario s/n, Moncada, 46113 Valencia, Spain.
authors.forename: Sagrario
authors.initials: S
authors.lastname: Torres-Cartas
authors.affiliation: Instituto de Investigación para la Gestión Integrada de Zonas Costeras, Universidad Politécnica de Valencia, C/Paranimf no.1, Grao de Gandía, 46730 Valencia, Spain; Departamento de Farmacia, Universidad CEU-Cardenal Herrera, Avenida Seminario s/n, Moncada, 46113 Valencia, Spain.
authors.forename: Susana
authors.initials: S
authors.lastname: Meseguer-Lloret
mesh_terms: D002563:Cerium / Q000737:chemistry*; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002853:Chromatography, Liquid; D049449:Luminescence; D009946:Organothiophosphorus Compounds / Q000032:analysis* / Q000737:chemistry; D010575:Pesticides / Q000032:analysis* / Q000737:chemistry; D014867:Water / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000737:chemistry
chemical_list: D009946:Organothiophosphorus Compounds; D010575:Pesticides; D014874:Water Pollutants, Chemical; D014867:Water; D002563:Cerium
keywords: Chemiluminescence detection; Liquid chromatography; Organothiophosphorus pesticides; Water