title: Chemical composition, antioxidant, anti-inflammatory and anti-proliferative activities of essential oils of plants from Burkina Faso.
abstract: This research highlights the chemical composition, antioxidant, anti-inflammatory and anti-proliferative activities of essential oils from leaves of Ocimum basilicum, Ocimum americanum, Hyptis spicigera, Lippia multiflora, Ageratum conyzoides, Eucalyptus camaldulensis and Zingiber officinale. Essential oils were analyzed by gas chromatography-mass spectrometry and gas chromatography-flame ionization detector. Major constituents were α-terpineol (59.78%) and β-caryophyllene (10.54%) for Ocimum basilicum; 1, 8-cineol (31.22%), camphor (12.730%), α-pinene (6.87%) and trans α-bergamotene (5.32%) for Ocimum americanum; β-caryophyllene (21%), α-pinene (20.11%), sabinene (10.26%), β-pinene (9.22%) and α-phellandrene (7.03%) for Hyptis spicigera; p-cymene (25.27%), β-caryophyllene (12.70%), thymol (11.88), γ-terpinene (9.17%) and thymyle acetate (7.64%) for Lippia multiflora; precocene (82.10%)for Ageratum conyzoides; eucalyptol (59.55%), α-pinene (9.17%) and limonene (8.76%) for Eucalyptus camaldulensis; arcurcumene (16.67%), camphene (12.70%), zingiberene (8.40%), β-bisabolene (7.83%) and β-sesquiphellandrène (5.34%) for Zingiber officinale. Antioxidant activities were examined using 1,1-diphenyl-2-picryl-hydrazyl (DPPH) and 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) methods. O. basilicum and L. multiflora exhibited the highest antioxidant activity in DPPH and ABTS tests, respectively. Anti-inflammatory properties were evaluated by measuring the inhibition of lipoxygenase activity and essential oil of Z. officinale was the most active. Anti-proliferative effect was assayed by the measurement of MTT on LNCaP and PC-3 prostate cancer cell lines, and SF-763 and SF-767 glioblastoma cell lines. Essential oils from A. conyzoides and L. multiflora were the most active on LNCaP and PC-3 cell lines, respectively. The SF-767 glioblastoma cell line was the most sensitive to O. basilicum and L. multiflora EOs while essential oil of A. conyzoides showed the highest activity on SF-763 cells. Altogether these results justify the use of these plants in traditional medicine in Burkina Faso and open a new field of investigation in the characterization of the molecules involved in anti-proliferative processes.
authors.affiliation: Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA), Laboratoire de Biologie et Génétique (LABIOGENE), Centre Médical Saint Camille, Ouagadougou, Université de Ouagadougou, Ouagadougou, Burkina Faso; Clermont Université, Université Blaise Pascal, Génétique Reproduction et Développement (GReD), Clermont-Ferrand, France; Centre National de la Recherche Scientifique (CNRS), Unité Mixte de Recherche (UMR) 6293, GReD, Aubière, France; Institut National de la Santé et de la Recherche Médicale (INSERM), UMR 1103, GReD, Aubière, France; Centre de Recherche en Nutrition Humaine d'Auvergne, Clermont-Ferrand, France.
authors.forename: Bagora
authors.initials: B
authors.lastname: Bayala
authors.affiliation: Laboratoire de Biochimie Alimentaire, Enzymologie, Biotechnologies industrielles et Bioinformatique (BAEBIB), Unité de Formation et de Recherche en Sciences de la Vie et de la Terre (UFR-SVT), Université de Ouagadougou, Ouagadougou, Burkina Faso.
authors.forename: Imaël Henri Nestor
authors.initials: IH
authors.lastname: Bassole
authors.affiliation: Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA), Laboratoire de Biologie et Génétique (LABIOGENE), Centre Médical Saint Camille, Ouagadougou, Université de Ouagadougou, Ouagadougou, Burkina Faso; Laboratoire de Pharmacologie, de Toxicologie et de Chimie Thérapeutique, Unité de Formation et de Recherche en Sciences de la Santé (UFR-SDS), Université de Ouagadougou, Ouagadougou, Burkina Faso.
authors.forename: Charlemagne
authors.initials: C
authors.lastname: Gnoula
authors.affiliation: Institut de Recherche en Sciences Appliquées et Techniques, Département des Substances Naturelles, Ouagadougou, Burkina Faso.
authors.forename: Roger
authors.initials: R
authors.lastname: Nebie
authors.affiliation: Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA), Laboratoire de Biologie et Génétique (LABIOGENE), Centre Médical Saint Camille, Ouagadougou, Université de Ouagadougou, Ouagadougou, Burkina Faso.
authors.forename: Albert
authors.initials: A
authors.lastname: Yonli
authors.affiliation: Clermont Université, Université Blaise Pascal, Génétique Reproduction et Développement (GReD), Clermont-Ferrand, France; Centre National de la Recherche Scientifique (CNRS), Unité Mixte de Recherche (UMR) 6293, GReD, Aubière, France; Institut National de la Santé et de la Recherche Médicale (INSERM), UMR 1103, GReD, Aubière, France; Centre de Recherche en Nutrition Humaine d'Auvergne, Clermont-Ferrand, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Morel
authors.affiliation: LEXVA Analytique, Biopole Clermont-Limagne, Saint-Beauzire, France.
authors.forename: Gilles
authors.initials: G
authors.lastname: Figueredo
authors.affiliation: Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA), Laboratoire de Biologie et Génétique (LABIOGENE), Centre Médical Saint Camille, Ouagadougou, Université de Ouagadougou, Ouagadougou, Burkina Faso; Centre Médical Saint Camille de Ouagadougou, Ouagadougou, Burkina Faso.
authors.forename: Jean-Baptiste
authors.initials: JB
authors.lastname: Nikiema
authors.affiliation: Clermont Université, Université Blaise Pascal, Génétique Reproduction et Développement (GReD), Clermont-Ferrand, France; Centre National de la Recherche Scientifique (CNRS), Unité Mixte de Recherche (UMR) 6293, GReD, Aubière, France; Institut National de la Santé et de la Recherche Médicale (INSERM), UMR 1103, GReD, Aubière, France; Centre de Recherche en Nutrition Humaine d'Auvergne, Clermont-Ferrand, France.
authors.forename: Jean-Marc A
authors.initials: JM
authors.lastname: Lobaccaro
authors.affiliation: Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA), Laboratoire de Biologie et Génétique (LABIOGENE), Centre Médical Saint Camille, Ouagadougou, Université de Ouagadougou, Ouagadougou, Burkina Faso; Centre Médical Saint Camille de Ouagadougou, Ouagadougou, Burkina Faso.
authors.forename: Jacques
authors.initials: J
authors.lastname: Simpore
mesh_terms: D000893:Anti-Inflammatory Agents / Q000737:chemistry* / Q000494:pharmacology*; D002050:Burkina Faso; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D016166:Free Radical Scavengers / Q000737:chemistry* / Q000494:pharmacology*; D006801:Humans; D009822:Oils, Volatile / Q000737:chemistry* / Q000494:pharmacology*; D010938:Plant Oils / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000893:Anti-Inflammatory Agents; D016166:Free Radical Scavengers; D009822:Oils, Volatile; D010938:Plant Oils