title: Neutralized nanoparticle composed of SS-cleavable and pH-activated lipid-like material as a long-lasting and liver-specific gene delivery system.
abstract: Charge-neutralized lipid envelope-type nanoparticles formed with SS-cleavable and pH-activated lipid-like materials (ssPalm) accumulate rapidly in the liver without forming aggregates in the blood circulation, and result in a liver-specific gene expression for a long duration (>2 weeks) with neither immunological responses nor hepatotoxicity after intraveneous administration, when it carries pDNA free from CpG-motifs.
authors.affiliation: Faculty of Pharmaceutical Sciences, Hokkaido University, Kita12 Nishi6, Kita-ku, Sapporo City, Hokkaido, 060-0812, Japan.
authors.forename: Masami
authors.initials: M
authors.lastname: Ukawa
authors.forename: Hidetaka
authors.initials: H
authors.lastname: Akita
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Hayashi
authors.forename: Ryohei
authors.initials: R
authors.lastname: Ishiba
authors.forename: Kota
authors.initials: K
authors.lastname: Tange
authors.forename: Masaya
authors.initials: M
authors.lastname: Arai
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Kubo
authors.forename: Yuriko
authors.initials: Y
authors.lastname: Higuchi
authors.forename: Kazunori
authors.initials: K
authors.lastname: Shimizu
authors.forename: Satoshi
authors.initials: S
authors.lastname: Konishi
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Hashida
authors.forename: Hideyoshi
authors.initials: H
authors.lastname: Harashima
mesh_terms: D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D001057:Apolipoproteins E / Q000235:genetics; D001219:Aspartate Aminotransferases / Q000378:metabolism; D004220:Disulfides / Q000737:chemistry*; D018014:Gene Transfer Techniques; D006863:Hydrogen-Ion Concentration; D008055:Lipids / Q000737:chemistry; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D053758:Nanoparticles / Q000737:chemistry / Q000378:metabolism* / Q000633:toxicity; D010316:Particle Size; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism; D011479:Protamines / Q000737:chemistry; D034741:RNA, Small Interfering / Q000378:metabolism
chemical_list: D001057:Apolipoproteins E; D004220:Disulfides; D008055:Lipids; D010714:Phosphatidylethanolamines; D011479:Protamines; D034741:RNA, Small Interfering; C097189:1-stearoyl-2-oleoylphosphatidylethanolamine; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase
keywords: CpG-free plasmid DNA; hepatic gene delivery; in vivo imaging; lipid-like material; neutralized particle