title: Proteomic analysis of rat prefrontal cortex after chronic valproate treatment.
abstract: Valproic acid (VPA) is commonly used to treat bipolar disorder (BD), but its therapeutic role has not been clearly elucidated. To gain insights into VPA's mechanism of action, proteomic analysis was used to identify differentially expressed proteins in the rat prefrontal cortex (PFC), a region particularly affected in BD, after 6 weeks of VPA treatment. Proteins from PFCs of control and VPA-treated rats were separated by 2D-DIGE and identified by mass spectrometry. Among the 2,826 protein spots resolved, the abundance of 19 proteins was found to be significantly altered in the VPA-treated group (with the levels of three proteins increasing and 16 decreasing). Seven proteins whose levels were significantly altered after chronic VPA exposure were quantified by Western blot analysis. The 19 identified proteins represent potential new targets for VPA action and should aid in our understanding of the role of VPA in BD.
authors.affiliation: Molecular Neuroscience and Bioinformatics Laboratories; Mental Health, Behavioral Science, and Research Services, Robley Rex Veterans Affairs Medical Center, Louisville, Kentucky; Department of Psychiatry and Behavioral Sciences, University of Louisville, Louisville, Kentucky.
authors.forename: Muthusamy
authors.initials: M
authors.lastname: Thangavel
authors.forename: Ratnam S
authors.initials: RS
authors.lastname: Seelan
authors.forename: Jaganathan
authors.initials: J
authors.lastname: Lakshmanan
authors.forename: Robert E
authors.initials: RE
authors.lastname: Vadnal
authors.forename: John I
authors.initials: JI
authors.lastname: Stagner
authors.forename: Latha K
authors.initials: LK
authors.lastname: Parthasarathy
authors.forename: Manuel F
authors.initials: MF
authors.lastname: Casanova
authors.forename: Rifaat Shody
authors.initials: RS
authors.lastname: El-Mallakh
authors.forename: Ranga N
authors.initials: RN
authors.lastname: Parthasarathy
mesh_terms: D000818:Animals; D000927:Anticonvulsants / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D005786:Gene Expression Regulation / Q000187:drug effects; D008297:Male; D008954:Models, Biological; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D017397:Prefrontal Cortex / Q000187:drug effects* / Q000378:metabolism; D060066:Protein Interaction Maps / Q000187:drug effects; D040901:Proteomics / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014635:Valproic Acid / Q000494:pharmacology*
chemical_list: D000927:Anticonvulsants; D009419:Nerve Tissue Proteins; D014635:Valproic Acid
keywords: 2D-DIGE; bipolar disorder; lithium; prefrontal cortex; valproate