title: Degradation of atrazine by Frankia alni ACN14a: gene regulation, dealkylation, and dechlorination.
abstract: Atrazine is transformed to N-isopropylammelide through hydroxyatrazine as an intermediate as indicated by high-performance liquid chromatography/mass spectroscopy in culture filtrates of Frankia alni ACN14a and Frankia sp. EuI1c. Both Frankia strains have the ability to degrade atrazine via dechlorination and dealkylation and, subsequently, may be using it as a nitrogen and carbon source as detected here by increasing their growth patterns. Bioinformatic analysis of the Frankia genomes revealed that a potential gene cluster involved in atrazine decomposition contains three genes, namely, trzN (FRAAL1474 and FraEuI1c_5874), atzB (FRAAL1473 and FraEuI1c_5875), and atzR (FRAAL1471). The relative messenger RNA gene expression of the former genes was examined by qRT-PCR. The LysR-type transcriptional regulator atzR (FRAAL1471), which is expected to control the cluster expression, showed a 13-fold increase in the expression level under atrazine stress. Moreover, the putative adenosine aminohydrolase 3 atzB (FRAAL1473), which is expected to dealkylate the N-ethyl group of atrazine, showed also an increased expression by factor 16 with increased exposure. Eventually, the trzN (FRAAL1474) gene, which is predicted to encode a putative amidohydrolase catalyzing atrazine dechlorination, exhibited 31-fold increased expression. To our best knowledge, this is the first report about adenosine aminohydrolase 3 function in the dealkylation of the N-ethyl group from atrazine.
authors.affiliation: Department of Bio- and Environmental Sciences, International Institute Zittau, Markt 23, 02763, Zittau, Germany, medhat.rehan@agr.kfs.edu.eg.
authors.forename: Medhat
authors.initials: M
authors.lastname: Rehan
authors.forename: Martin
authors.initials: M
authors.lastname: Kluge
authors.forename: Stefan
authors.initials: S
authors.lastname: Fränzle
authors.forename: Harald
authors.initials: H
authors.lastname: Kellner
authors.forename: René
authors.initials: R
authors.lastname: Ullrich
authors.forename: Martin
authors.initials: M
authors.lastname: Hofrichter
mesh_terms: D001280:Atrazine / Q000378:metabolism*; D001711:Biotransformation; D002244:Carbon / Q000378:metabolism; D002713:Chlorine / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D019295:Computational Biology; D003640:Dealkylation; D040161:Frankia / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D013058:Mass Spectrometry; D005810:Multigene Family; D009584:Nitrogen / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D002713:Chlorine; D002244:Carbon; D009584:Nitrogen; D001280:Atrazine