title: Interferon lambda-2 levels in sputum of patients with pulmonary Mycobacterium tuberculosis infection.
abstract: In humans, there are three members of type III interferon family, designated as IFN-λ1 (IL-29), IFN-λ2 (IL-28A) and IFN-λ3 (IL-28B). Bacterial infections, including Mycobacterium tuberculosis infection, trigger the expression of interferon I and II genes, but little is known about their effect on IFN-λ genes. It has been found that M. tuberculosis infection in A549 lung epithelial cells stimulate upregulation of IFN-λ2 genes in vitro. The aim of this study was to determine the IFN-λ2 levels in sputum of patients with active pulmonary tuberculosis comparing with levels in healthy persons sputum and sputum of persons with latent tuberculosis. IFN-λ2 level in the samples was quantified with sandwich enzyme-linked immunoassay kit for quantization of human IFN-λ2 (IL-28A). The IFN-λ2 level in sputum of patients with pulmonary tuberculosis was significantly higher comparing with IFN-λ2 levels in healthy group (P = 0.001) and latent tuberculosis group (P = 0.005). Logistic regression analysis has shown that IFN-λ2 level in sputum is related to culture result (OR = 4.14; 95% CI = 1.28-17.5; P = 0.019). There was the correlation between Bartlett score and IFN-λ2 levels in group with pulmonary tuberculosis (r = 0.641; P < 0.01). In conclusion, we have found that the inflammatory cells in sputum can be source of IFN-λ after receptor stimulation by M. tuberculosis components.
authors.affiliation: Department of Microbiology and Immunology, Faculty of Medicine, Banja Luka University, Banja Luka, Republic of Srpska, Bosnia and Herzegovina; Department of Microbiology, Clinic Centre Banja Luka, Banja Luka, Republic of Srpska, Bosnia and Herzegovina.
authors.forename: M
authors.initials: M
authors.lastname: Travar
authors.forename: M
authors.initials: M
authors.lastname: Vucic
authors.forename: M
authors.initials: M
authors.lastname: Petkovic
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D007378:Interleukins / Q000032:analysis*; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D013183:Sputum / Q000276:immunology*; D014397:Tuberculosis, Pulmonary / Q000276:immunology*; D000095022:Interferon Lambda
chemical_list: D007378:Interleukins; C000624353:interferon-lambda, human; D000095022:Interferon Lambda