title: Lycopene cyclase and phytoene synthase activities in the marine yeast Rhodosporidium diobovatum are encoded by a single gene crtYB.
abstract: crtYB, encoding lycopene cyclase and phytoene synthase was cloned from Rhodosporidium diobovatum ATCC 2527 by rapid amplification of cDNA ends method. The full-length cDNA of crtYB is 2, 330 bp and contains eight introns. The gene products is a 594 amino acids, with a predicted molecular mass of 65.63 kDa and a pI of 6.73. The N-terminus of the protein contains six transmembrane regions, which has been characterized as a lycopene beta-cyclase. The C-terminal half has squalene and phytoene synthase signatures that identified as phytoene synthetase. By heterologous complementary detection of this gene in E. coli and HPLC analysis, the regions responsible for phytoene synthesis and lycopene cyclization were localized within the protein.
authors.affiliation: Key Discipline of Biological Engineering of Hebei Province, College of Life Sciences, Hebei University, Baoding, China; Key Laboratory of Microbial Diversity Research and Application of Hebei Province, College of Life Sciences, Hebei University, Baoding, China.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Guo
authors.forename: Hui
authors.initials: H
authors.lastname: Tang
authors.forename: Liping
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001487:Basidiomycota / Q000201:enzymology* / Q000235:genetics; D003001:Cloning, Molecular / Q000379:methods; D005800:Genes, Fungal; D051232:Geranylgeranyl-Diphosphate Geranylgeranyltransferase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D019753:Intramolecular Lyases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data
chemical_list: D051232:Geranylgeranyl-Diphosphate Geranylgeranyltransferase; D019753:Intramolecular Lyases; C070925:lycopene cyclase-isomerase
keywords: Bifunctional gene; Carotenoids; Genetic complementation; Lycopene cyclase; Phytoene synthase; Rhodosporidium diobovatum