title: Morphological and glycan features of the camel oviduct epithelium.
abstract: This study describes regional differences in the oviduct of the one-humped camel (Camelus dromedarius) during the growth phase (GP) and the mature phase (MP) of the follicular wave by means of morphometry, scanning electron microscopy (SEM) and glycohistochemistry investigations. Epithelium height significantly increased in the ampulla and decreased in the isthmus passing from the GP to the MP. Under SEM, non-ciliated cells displayed apical blebs (secretory) or short microvilli. Cilia glycocalyx expressed glycans terminating with sialic acid linked α2,6 to Gal/GalNAc (SNA affinity) throughout the oviducts of GP and MP and sialic acid linked α2,3 to Galβ1,3GalNAc (MAL II and KOH-sialidase (K-s)-PNA staining) throughout the MP oviducts. Non-ciliated cells displayed lectin-binding sites from the supra-nuclear cytoplasm to the luminal surface. Ampulla non-ciliated cells showed O-linked (mucin-type) sialoglycans (MAL II and K-s-PNA) during GP and MP and N-linked sialoglycans (SNA) during the MP. Isthmus non-ciliated cells expressed SNA reactivity in GP and MP, also K-s-PNA binders in MP, and MAL II and PNA affinity (Galβ1,3GalNAc) during GP. Galβ1,3GalNAc was sialilated in the non-ciliated cells of GP UTJ. Luminal surface lacked of Galβ1,3GalNAc in GP and MP, whereas it expressed α2,6- and α2,3-linked sialic acids. In GP intraluminal substance reacted with SNA, MAL II, K-s-PNA in ampulla and only with MAL II in the isthmus and UTJ. These results demonstrate that the morphology and the glycan pattern of the camel oviductal epithelium vary during the follicular wave and that could relate to the region-specific functions.
authors.affiliation: Veterinary Clinic and Animal Productions Section, Department of Emergency and Organ Trasplantations (DETO), University of Bari "Aldo Moro", Italy.
authors.forename: Gianluca
authors.initials: G
authors.lastname: Accogli
authors.affiliation: Veterinary Clinic and Animal Productions Section, Department of Emergency and Organ Trasplantations (DETO), University of Bari "Aldo Moro", Italy.
authors.forename: Davide
authors.initials: D
authors.lastname: Monaco
authors.affiliation: Desert Research Center (DRC), Animal Production and Poultry Division, Cairo, Egypt.
authors.forename: Khalid Ahmed
authors.initials: KA
authors.lastname: El Bahrawy
authors.affiliation: Cairo University Research Park (CURP), Faculty of Agriculture, Cairo University, 12613, Giza, Egypt; Department of Animal Production, Faculty of Agriculture, Cairo University, 12613, Giza, Egypt.
authors.forename: Ashraf Abd El-Halim
authors.initials: AA
authors.lastname: El-Sayed
authors.affiliation: Veterinary Clinic and Animal Productions Section, Department of Emergency and Organ Trasplantations (DETO), University of Bari "Aldo Moro", Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Ciannarella
authors.affiliation: SupAgro, Systèmes d'Elevage Méditerranéens et Tropicaux, UMR-SELMET, Montpellier, France.
authors.forename: Benedicte
authors.initials: B
authors.lastname: Beneult
authors.affiliation: Veterinary Clinic and Animal Productions Section, Department of Emergency and Organ Trasplantations (DETO), University of Bari "Aldo Moro", Italy.
authors.forename: Giovanni Michele
authors.initials: GM
authors.lastname: Lacalandra
authors.affiliation: Veterinary Clinic and Animal Productions Section, Department of Emergency and Organ Trasplantations (DETO), University of Bari "Aldo Moro", Italy. Electronic address: salvatore.desantis@uniba.it.
authors.forename: Salvatore
authors.initials: S
authors.lastname: Desantis
mesh_terms: D000818:Animals; D002162:Camelus / Q000502:physiology*; D004848:Epithelium / Q000378:metabolism*; D005187:Fallopian Tubes / Q000033:anatomy & histology* / Q000378:metabolism*; D005260:Female; D011134:Polysaccharides / Q000378:metabolism*; D014599:Uterus / Q000378:metabolism
chemical_list: D011134:Polysaccharides
keywords: Camel; Follicular wave; Glycoproteins; Lectin histochemistry; Oviduct; SEM; Sialic acid