title: Two novel Saccharomycopsis species isolated from black olive brines and a tropical plant. Description of Saccharomycopsis olivae f. a., sp. nov. and Saccharomycopsis guyanensis f. a., sp. nov. Reassignment of Candida amapae to Saccharomycopsis amapae f. a., comb. nov., Candida lassenensis to Saccharomycopsis lassenensis f. a., comb. nov. and Arthroascus babjevae to Saccharomycopsis babjevae f. a., comb. nov.
abstract: Three yeast strains related to members of the genus Saccharomycopsis were isolated. One strain (CLIB 1310) was isolated from olive brines of fermented black olives in France and two strains (CLIB 1454 and CLIB 1455) were isolated from a plant in French Guiana. Sequence analyses based on the D1/D2 domains of the nuclear large subunit rRNA gene, small-subunit rRNA gene and partial EF-1α gene revealed that the strains represented two novel taxa exhibiting extensive sequence divergence from the previously described species of the genus Saccharomycopsis. Two novel species are described to accommodate these newly isolated strains: Saccharomycopsis olivae sp. nov. (type strain CLIB 1310(T) = CBS 12701(T)) and Saccharomycopsis guyanensis sp. nov. (type strain CLIB 1455(T) = CBS 12914(T) and strain CLIB 1454). Both strains CLIB 1454 and CLIB 1455(T) displayed identical sequences but differed in their ability to metabolize sorbitol and in their morphology on agar medium. Candida amapae, Candida lassensensis and Arthroascus babjevae belonging to the Saccharomycopsis clade, are reassigned to Saccharomycopsis as novel combinations.
authors.affiliation: AgroParisTech UMR1319, Micalis Institute, 78850 F-Thiverval-Grignon, France.|INRA UMR1319, Micalis Institute, CIRM-Levures, 78850 F-Thiverval-Grignon, France.
authors.forename: Noémie
authors.initials: N
authors.lastname: Jacques
authors.affiliation: AgroParisTech UMR1319, Micalis Institute, 78850 F-Thiverval-Grignon, France.|INRA UMR1319, Micalis Institute, CIRM-Levures, 78850 F-Thiverval-Grignon, France.
authors.forename: Christelle
authors.initials: C
authors.lastname: Louis-Mondesir
authors.affiliation: Université de Brest, EA3882, Laboratoire Universitaire de Biodiversité et d'Ecologie Microbienne, ESIAB, Technopôle Brest Iroise, 29280 - Plouzané, France.
authors.forename: Monika
authors.initials: M
authors.lastname: Coton
authors.affiliation: Université de Brest, EA3882, Laboratoire Universitaire de Biodiversité et d'Ecologie Microbienne, ESIAB, Technopôle Brest Iroise, 29280 - Plouzané, France.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Coton
authors.affiliation: AgroParisTech UMR1319, Micalis Institute, 78850 F-Thiverval-Grignon, France.|INRA UMR1319, Micalis Institute, CIRM-Levures, 78850 F-Thiverval-Grignon, France.
authors.forename: Serge
authors.initials: S
authors.lastname: Casaregola
mesh_terms: D000818:Animals; D004271:DNA, Fungal / Q000235:genetics; D005285:Fermentation; D005602:France; D005616:French Guiana; D049851:Gastropoda / Q000235:genetics; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D031658:Olea / Q000382:microbiology*; D020648:Peptide Elongation Factor 1 / Q000235:genetics; D010802:Phylogeny; D012335:RNA, Ribosomal / Q000235:genetics; D012442:Saccharomycopsis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012492:Salts; D017422:Sequence Analysis, DNA
chemical_list: D004271:DNA, Fungal; D020648:Peptide Elongation Factor 1; D012335:RNA, Ribosomal; D012492:Salts; C017082:brine