title: Protective effect of hydrogen sulfide on gentamicin-induced renal injury.
abstract: OBJECTIVE
  We evaluated the potential protective effect of hydrogen sulfide (H2S) against GEN-induced nephrotoxicity in rats.
  
  
  METHODS
  Twenty-four rats were randomly divided into four groups, each consisting of six animals as follows: (1) the rats were control, (2) intraperitoneally injected with GEN 14 consecutive days (100 mg/kg/day), (3) treated with GEN plus %0.9 saline intraperitoneally for 14 days and (4) treated with GEN plus sodium hydrogen sulfide (NaHS)-exogenous H2S donor (56 µmol/kg/day) for 14 days. After 15 days, rats were sacrificed and their kidneys were taken and blood analysis was performed. Twenty-four hours urine collections were obtained in standard metabolic cages a day before the rats were sacrificed. Tubular necrosis and interstitial fibrosis scoring were determined histopathologically in a part of kidneys; nitric oxide (NO), malondialdehyde (MDA) and reduced glutathione (GSH) levels were determined in the other part of kidneys. Statistical analyses were made by the chi-squared test and one-way analysis of variance.
  
  
  RESULTS
  Serum urea and creatinine levels were significantly higher in rats treated with GEN alone, than the rats in control and GEN + NaHS groups. The GSH levels in renal tissue of only GEN-treated rats were significantly lower than those in control group, and administration of NaHS to GEN-treated rats significantly increased the level of GSH. The group that was given GEN and NaHS had significantly lower MDA and NO levels in kidney cortex tissue than those that was given GEN alone. In rats treated with GEN + NaHS, despite the presence of mild tubular degeneration and tubular necrosis are less severe, and glomeruli maintained a better morphology when compared with GEN group.
  
  
  CONCLUSIONS
  We can say that H2S prevent kidney damage with antioxidant and anti-inflammatory effect.
authors.affiliation: Department Urology, Okmeydani Training and Research Hospital , Istanbul , Turkey .
authors.forename: Alper
authors.initials: A
authors.lastname: Otunctemur
authors.forename: Emin
authors.initials: E
authors.lastname: Ozbek
authors.forename: Murat
authors.initials: M
authors.lastname: Dursun
authors.forename: Suleyman
authors.initials: S
authors.lastname: Sahin
authors.forename: Huseyin
authors.initials: H
authors.lastname: Besiroglu
authors.forename: Ozgur Doga
authors.initials: OD
authors.lastname: Ozsoy
authors.forename: Mustafa
authors.initials: M
authors.lastname: Cekmen
authors.forename: Adnan
authors.initials: A
authors.lastname: Somay
authors.forename: Nurver
authors.initials: N
authors.lastname: Ozbay
mesh_terms: D058186:Acute Kidney Injury / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control* / Q000652:urine; D000818:Animals; D004353:Drug Evaluation, Preclinical; D005839:Gentamicins; D006862:Hydrogen Sulfide / Q000627:therapeutic use*; D007668:Kidney / Q000473:pathology; D008297:Male; D011897:Random Allocation; D017208:Rats, Wistar
chemical_list: D005839:Gentamicins; D006862:Hydrogen Sulfide
keywords: Gentamicin; hydrogen sulfide; kidney; nephrotoxicity; oxidative stress