title: Comparison of physicochemical properties and immunomodulatory activity of polysaccharides from fresh and dried litchi pulp.
abstract: Drying is commonly used for preservation and processing of litchi. However, its polysaccharide structure may be altered by the drying process, resulting in biological activity changes. Polysaccharides from fresh and dried litchi pulp (denoted as LPF and LPD, respectively) were isolated, investigated by GC-MS, GPC and UV/IR spectrum analysis and their antitumor and immunomodulatory activities were evaluated in vitro. LPD, the molecular weight of which was lower than that of LPF, contained more protein, uronic acid, arabinose, galactose and xylose. Compared with LPF, LPD exhibited a higher inhibitory effect on the proliferation of HepG2, Hela and A549 cells from 50-750 μg/mL. LPD was also a better stimulator of spleen lymphocyte proliferation, NK cells cytotoxicity and macrophage phagocytosis from 50-400 μg/mL. In summary, drying could change the physicochemical properties and enhance the bioactivity of polysaccharides from litchi pulp. This finding is supported by the fact that dried litchi pulps are used in Traditional Chinese Medicine.
authors.affiliation: Department of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Huang
authors.affiliation: Sericultural and Agri-food Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510610, China.
authors.forename: Ruifen
authors.initials: R
authors.lastname: Zhang
authors.affiliation: College of Food Science & Engineering, Wuhan Polytechnic University, Wuhan 430023, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yi
authors.affiliation: Sericultural and Agri-food Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510610, China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Tang
authors.affiliation: Sericultural and Agri-food Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510610, China. mwzhh@vip.tom.com.
authors.forename: Mingwei
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Department of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Dongxiao
authors.initials: D
authors.lastname: Su
authors.affiliation: Sericultural and Agri-food Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510610, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Deng
authors.affiliation: Sericultural and Agri-food Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510610, China.
authors.forename: Zhencheng
authors.initials: Z
authors.lastname: Wei
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D001089:Arabinose / Q000302:isolation & purification; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003890:Desiccation; D005638:Fruit / Q000737:chemistry*; D005690:Galactose / Q000302:isolation & purification; D056945:Hep G2 Cells; D006801:Humans; D007155:Immunologic Factors / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D007694:Killer Cells, Natural / Q000166:cytology / Q000187:drug effects; D032125:Litchi / Q000737:chemistry*; D008264:Macrophages / Q000166:cytology / Q000187:drug effects; D051379:Mice; D010587:Phagocytosis / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry; D010940:Plant Proteins / Q000302:isolation & purification; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D013154:Spleen / Q000166:cytology / Q000187:drug effects; D014574:Uronic Acids / Q000302:isolation & purification; D014994:Xylose / Q000302:isolation & purification
chemical_list: D000970:Antineoplastic Agents; D007155:Immunologic Factors; D010936:Plant Extracts; D010940:Plant Proteins; D011134:Polysaccharides; D014574:Uronic Acids; D014994:Xylose; D001089:Arabinose; D005690:Galactose