title: Comparative transcriptome analysis of sex pheromone glands of two sympatric lepidopteran congener species.
abstract: Two internal apple feeders of Grapholita molesta and Grapholita dimorpha share two major sex pheromone components (stereoisomers) and exhibit a similar circadian rhythm of mating behavior. This study aimed to determine the genetic factors diversifying these two congeners with respect to sex pheromone biosynthetic machinery. Transcriptomes of sex pheromone glands in both species were analyzed with a deep sequencing technology. To find out the gene(s) responsible for the stereoisomer ratios of G. molesta and G. dimorpha, a hypothetic sex pheromone biosynthetic pathway was predicted based on the transcriptomes of their sex pheromone glands. Some orthologs of Δ10 desaturase and FARs in the synthetic pathway were specifically expressed in sex pheromone glands. The relatively high variation in DNA sequence and expression levels between G. molesta and G. dimorpha suggest their crucial roles in generating differential ratios of the sex pheromone stereoisomers in these two sympatric congeners.
authors.affiliation: Department of Bioresource Sciences, Andong National University, Andong 760-749, Republic of Korea.
authors.forename: Chung Ryul
authors.initials: CR
authors.lastname: Jung
authors.affiliation: Department of Bioresource Sciences, Andong National University, Andong 760-749, Republic of Korea. Electronic address: hosanna@andong.ac.kr.
authors.forename: Yonggyun
authors.initials: Y
authors.lastname: Kim
mesh_terms: D000445:Aldehyde Oxidoreductases / Q000235:genetics; D000818:Animals; D044943:Fatty Acid Desaturases / Q000235:genetics; D014644:Genetic Variation; D007915:Lepidoptera / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D012543:Scent Glands / Q000502:physiology*; D012724:Sex Attractants / Q000096:biosynthesis*; D059467:Transcriptome
chemical_list: D012724:Sex Attractants; D044943:Fatty Acid Desaturases; C467901:acyl-CoA delta10-desaturase; D000445:Aldehyde Oxidoreductases
keywords: Desaturase; Grapholita; Sex pheromone; Speciation; Transcriptome