title: Cashew apple juice as microbial cultivation medium for non-immunogenic hyaluronic acid production.
abstract: In this work, natural cashew apple juice was used as cultivation medium as an alternative to substitute brain heart infusion medium. The effect of aeration and juice supplementation with yeast extract on the production of hyaluronic acid in batch fermentation was also investigated. Similar levels of cell mass were obtained in inoculum using cashew apple juice supplemented with yeast extract or the conventional brain heart infusion medium. Fermentation in Erlenmeyer flasks produced low biomass and hyaluronic acid concentrations. The hyaluronic acid concentration and viscosity increased from 0.15 g/L and 3.87 cP (no aeration or medium supplementation) to 1.76 g/L and 107 cP, when aeration (2 vvm) and 60 g/L of yeast extract were used. The results suggest the production of low-molecular weight hyaluronic acid oligomers instead of the high molecular weight polymer.
authors.affiliation: Departamento de Engenharia Química, Universidade Federal do Ceará, Fortaleza, CE, Brazil.
authors.forename: Adriano H
authors.initials: AH
authors.lastname: Oliveira
authors.affiliation: Faculdade de Engenharia Química, Universidade Estadual de Campinas, Campinas, SP, Brazil.
authors.forename: Cristiane C
authors.initials: CC
authors.lastname: Ogrodowski
authors.affiliation: Departamento de Engenharia Química, Universidade Federal do Ceará, Fortaleza, CE, Brazil.
authors.forename: André C
authors.initials: AC
authors.lastname: de Macedo
authors.affiliation: Faculdade de Engenharia Química, Universidade Estadual de Campinas, Campinas, SP, Brazil.
authors.forename: Maria Helena A
authors.initials: MH
authors.lastname: Santana
authors.affiliation: Departamento de Engenharia Química, Universidade Federal do Ceará, Fortaleza, CE, Brazil.
authors.forename: Luciana R B
authors.initials: LR
authors.lastname: Gonçalves
mesh_terms: D031021:Anacardium / Q000737:chemistry*; D018533:Biomass; D003470:Culture Media / Q000737:chemistry*; D005285:Fermentation; D006820:Hyaluronic Acid / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000254:growth & development* / Q000378:metabolism*
chemical_list: D003470:Culture Media; D006820:Hyaluronic Acid
keywords: cashew apple juice; hyaluronic acid; submerged fermentation