title: Antimicrobial prenylated benzoylphloroglucinol derivatives and xanthones from the leaves of Garcinia goudotiana.
abstract: Bioassay-guided fractionation using antimicrobial assay of the crude acetonic extract of Garcinia goudotiana leaves and of its five partitions led to the isolation of two new prenylated benzoylphloroglucinol derivatives, goudotianone 1 (1) and goudotianone 2 (2), in addition to two known compounds including one xanthone, 1,3,7-trihydroxy-2-isoprenylxanthone (3), and one triterpenoid, friedelin (4). Their structures were elucidated on the basis of different spectroscopic methods, including extensive 1D- and 2D-NMR spectroscopy and mass spectrometry. The crude acetonic extract, the methylene chloride and ethyl acetate partitions, and some tested compounds isolated from this species (1-3) demonstrated selective significant antimicrobial activities against Gram-positive bacteria, in particular Staphylococcus lugdunensis, Enterococcus faecalis and Mycobacterium smegmatis. The potential cytotoxic activities of these extracts and compounds were evaluated against human colon carcinoma HT29 and human fetal lung fibroblast MRC5 cells.
authors.affiliation: Laboratoire de Chimie Appliquée aux Substances Naturelles, Faculté des Sciences, Université d'Antananarivo, BP 566, Antananarivo 101, Madagascar; Institut Régional de Recherche en Agroalimentaire et Biotechnologie: Charles Viollette, EA1026, Université Lille 2, F-59000 Lille, France; EA GRIIOT, EA4481, Laboratoire de pharmacognosie, UFR Pharmacie, Université Lille 2, F-59000 Lille, France.
authors.forename: Sania
authors.initials: S
authors.lastname: Mahamodo
authors.affiliation: Institut Régional de Recherche en Agroalimentaire et Biotechnologie: Charles Viollette, EA1026, Université Lille 2, F-59000 Lille, France; EA GRIIOT, EA4481, Laboratoire de pharmacognosie, UFR Pharmacie, Université Lille 2, F-59000 Lille, France. Electronic address: celine.riviere-3@univ-lille2.fr.
authors.forename: Céline
authors.initials: C
authors.lastname: Rivière
authors.affiliation: INSERM U995, UFR Pharmacie, Université Lille 2, F-59000 Lille, France.
authors.forename: Christel
authors.initials: C
authors.lastname: Neut
authors.affiliation: Institut Régional de Recherche en Agroalimentaire et Biotechnologie: Charles Viollette, EA1026, Université Lille 2, F-59000 Lille, France; EA GRIIOT, EA4481, Laboratoire de pharmacognosie, UFR Pharmacie, Université Lille 2, F-59000 Lille, France.
authors.forename: Amin
authors.initials: A
authors.lastname: Abedini
authors.affiliation: University of Missouri, 1 University Boulevard, St. Louis, MO 63121, USA.
authors.forename: Heritiana
authors.initials: H
authors.lastname: Ranarivelo
authors.affiliation: CUMA, UFR Pharmacie, Université Lille 2, F-59000 Lille, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Duhal
authors.affiliation: Institut Régional de Recherche en Agroalimentaire et Biotechnologie: Charles Viollette, EA1026, Université Lille 2, F-59000 Lille, France; EA GRIIOT, EA4481, Laboratoire de pharmacognosie, UFR Pharmacie, Université Lille 2, F-59000 Lille, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Roumy
authors.affiliation: Institut Régional de Recherche en Agroalimentaire et Biotechnologie: Charles Viollette, EA1026, Université Lille 2, F-59000 Lille, France; EA GRIIOT, EA4481, Laboratoire de pharmacognosie, UFR Pharmacie, Université Lille 2, F-59000 Lille, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Hennebelle
authors.affiliation: Institut Régional de Recherche en Agroalimentaire et Biotechnologie: Charles Viollette, EA1026, Université Lille 2, F-59000 Lille, France; EA GRIIOT, EA4481, Laboratoire de pharmacognosie, UFR Pharmacie, Université Lille 2, F-59000 Lille, France.
authors.forename: Sevser
authors.initials: S
authors.lastname: Sahpaz
authors.affiliation: EA GRIIOT, EA4481, Laboratoire de pharmacognosie, UFR Pharmacie, Université Lille 2, F-59000 Lille, France.
authors.forename: Amélie
authors.initials: A
authors.lastname: Lemoine
authors.affiliation: Laboratoire de Chimie Appliquée aux Substances Naturelles, Faculté des Sciences, Université d'Antananarivo, BP 566, Antananarivo 101, Madagascar.
authors.forename: Dorothée
authors.initials: D
authors.lastname: Razafimahefa
authors.affiliation: Laboratoire de Chimie Appliquée aux Substances Naturelles, Faculté des Sciences, Université d'Antananarivo, BP 566, Antananarivo 101, Madagascar.
authors.forename: Bakonirina
authors.initials: B
authors.lastname: Razanamahefa
authors.affiliation: Institut Régional de Recherche en Agroalimentaire et Biotechnologie: Charles Viollette, EA1026, Université Lille 2, F-59000 Lille, France; EA GRIIOT, EA4481, Laboratoire de pharmacognosie, UFR Pharmacie, Université Lille 2, F-59000 Lille, France.
authors.forename: François
authors.initials: F
authors.lastname: Bailleul
authors.affiliation: Laboratoire de Chimie Appliquée aux Substances Naturelles, Faculté des Sciences, Université d'Antananarivo, BP 566, Antananarivo 101, Madagascar.
authors.forename: Bakolinirina
authors.initials: B
authors.lastname: Andriamihaja
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D028084:Clusiaceae / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D006094:Gram-Positive Bacteria / Q000187:drug effects*; D019073:HT29 Cells; D006801:Humans; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D010696:Phloroglucinol / Q000031:analogs & derivatives / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D013329:Structure-Activity Relationship; D044004:Xanthones / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D044004:Xanthones; D010696:Phloroglucinol
keywords: Antimicrobial activities; Benzoylphloroglucinol derivatives; Clusiaceae Madagascar; Cytotoxic activities; Garcinia goudotiana; Xanthones