title: Free radical scavenging activity and reducing power of Acacia nilotica wood lignin.
abstract: Nine different fractions of lignin extracted by alkali, hot water and organosolv methods from Acacia wood powder were assessed for antioxidants activity. Results indicated that methanolic lignin fraction had highest polyphenol content of 393.30±9.2μg/ml (GAE). The oraganosolv lignin with total phenols and phenolic hydroxyl group content exhibited significant antioxidant activity as compared to other lignin fractions. Antioxidant properties of acetone fractions revealed a high antiradical scavenging activity (<90%) with a simultaneous high ferric and molybdate ion reducing capacity. The influence of extraction methods on functional groups of lignin fractions was confirmed by analytical methods and Fourier transform infrared spectroscopy (FTIR) analysis. Whereas the phenolic content showed strong correlation with reducing capacity, the antiradical activity was moderately correlated with phenolic content. A high phenolic hydroxyl groups content of organosolv lignin fractions provides evidence for the presence of active therapeutic antioxidant compounds for their testing as potential value added products for cosmetics and pharmaceutical industries.
authors.affiliation: Department of Biotechnology, Guru Ghasidas Vishwavidyalaya, (Central University) Bilaspur 495009, Chhattisgarh, India.
authors.forename: Keshaw Ram
authors.initials: KR
authors.lastname: Aadil
authors.affiliation: Department of Biotechnology, Guru Ghasidas Vishwavidyalaya, (Central University) Bilaspur 495009, Chhattisgarh, India.
authors.forename: Anand
authors.initials: A
authors.lastname: Barapatre
authors.affiliation: Department of Biotechnology, Guru Ghasidas Vishwavidyalaya, (Central University) Bilaspur 495009, Chhattisgarh, India.
authors.forename: Sudha
authors.initials: S
authors.lastname: Sahu
authors.affiliation: Department of Biotechnology, Guru Ghasidas Vishwavidyalaya, (Central University) Bilaspur 495009, Chhattisgarh, India. Electronic address: harit74@yahoo.co.in.
authors.forename: Harit
authors.initials: H
authors.lastname: Jha
authors.affiliation: Department of Biotechnology, Guru Ghasidas Vishwavidyalaya, (Central University) Bilaspur 495009, Chhattisgarh, India.
authors.forename: Bhupendra Nath
authors.initials: BN
authors.lastname: Tiwary
mesh_terms: D000045:Acacia / Q000737:chemistry*; D000975:Antioxidants / Q000737:chemistry; D016166:Free Radical Scavengers / Q000737:chemistry*; D006801:Humans; D008031:Lignin / Q000737:chemistry* / Q000302:isolation & purification; D010084:Oxidation-Reduction; D010636:Phenols / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry*; D014934:Wood / Q000737:chemistry
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D010636:Phenols; D010936:Plant Extracts; D008031:Lignin
keywords: Acacia nilotica; Antioxidant activity; Lignin