title: The FTO gene polymorphism (rs9939609) is associated with metabolic syndrome in morbidly obese subjects from southern Italy.
abstract: Gene variants in MC4R, SIRT1 and FTO are associated with severe obesity and metabolic impairment in Caucasians. We investigated whether common variants in these genes are associated with metabolic syndrome (MetS) in a large group of morbidly obese young adults from southern Italy. One thousand morbidly obese subjects (62% women, mean body mass index 46.5 kg/m(2), mean age 32.6 years) whose families had lived in southern Italy for at least 2 generations were recruited. Single-nucleotide polymorphisms (SNPs) rs12970134, rs477181, rs502933 (MC4R locus), rs3818292, rs7069102, rs730821, rs2273773, rs12413112 (SIRT1 locus) and rs1421085, rs9939609, 9930506, 1121980 (FTO locus) were genotyped by Taqman assay; blood parameters were assayed by routine methods; the Fat Mass, Fat Free Mass, Respiratory Quotient, Basal Metabolic Rate (BMR) and waist circumference were also determined. Binomial logistic regression showed that the TA heterozygous genotype of SNP rs9939609 in the FTO gene was associated with the presence of MetS in our population [OR (95% CI): 2.53 (1.16-5.55)]. Furthermore, the FTO rs9939609 genotype accounted for 21.3% of the MetS phenotype together with total cholesterol, BMR and age. Our results extend the knowledge on genotype susceptibility for MetS in relation to a specific geographical area of residence.
authors.affiliation: CEINGE Biotecnologie Avanzate S.C. a. r.l., Naples, Italy; Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Università degli Studi di Napoli Federico II, Naples, Italy.
authors.forename: Rosario
authors.initials: R
authors.lastname: Liguori
authors.affiliation: IRCCS Fondazione SDN, Istituto di Ricerca Diagnostica e Nucleare, Naples, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Labruna
authors.affiliation: CEINGE Biotecnologie Avanzate S.C. a. r.l., Naples, Italy; Dipartimento di Scienze Motorie e del Benessere, Università degli Studi di Napoli "Parthenope", Naples, Italy.
authors.forename: Andreina
authors.initials: A
authors.lastname: Alfieri
authors.affiliation: CEINGE Biotecnologie Avanzate S.C. a. r.l., Naples, Italy; Dipartimento di Scienze Motorie e del Benessere, Università degli Studi di Napoli "Parthenope", Naples, Italy.
authors.forename: Domenico
authors.initials: D
authors.lastname: Martone
authors.affiliation: Dipartimento di Sanità Pubblica, Università degli Studi di Napoli Federico II, Naples, Italy.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Farinaro
authors.affiliation: Centro Interuniversitario di Studi e Ricerche sull'Obesità (CISRO) e Dipartimento di Medicina Clinica e Chirurgia, Università degli Studi di Napoli Federico II, Naples, Italy.
authors.forename: Franco
authors.initials: F
authors.lastname: Contaldo
authors.affiliation: CEINGE Biotecnologie Avanzate S.C. a. r.l., Naples, Italy; Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Università degli Studi di Napoli Federico II, Naples, Italy. Electronic address: sacchett@unina.it.
authors.forename: Lucia
authors.initials: L
authors.lastname: Sacchetti
authors.affiliation: Centro Interuniversitario di Studi e Ricerche sull'Obesità (CISRO) e Dipartimento di Medicina Clinica e Chirurgia, Università degli Studi di Napoli Federico II, Naples, Italy.
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Pasanisi
authors.affiliation: CEINGE Biotecnologie Avanzate S.C. a. r.l., Naples, Italy; Dipartimento di Scienze Motorie e del Benessere, Università degli Studi di Napoli "Parthenope", Naples, Italy. Electronic address: buono@uniparthenope.it.
authors.forename: Pasqualina
authors.initials: P
authors.lastname: Buono
mesh_terms: D000328:Adult; D000071516:Alpha-Ketoglutarate-Dependent Dioxygenase FTO; D005260:Female; D056726:Genetic Association Studies; D014644:Genetic Variation; D006801:Humans; D007558:Italy; D016015:Logistic Models; D008297:Male; D024821:Metabolic Syndrome / Q000150:complications* / Q000235:genetics* / Q000473:pathology; D009767:Obesity, Morbid / Q000235:genetics* / Q000473:pathology; D020641:Polymorphism, Single Nucleotide; D011506:Proteins / Q000235:genetics*; D044105:Receptor, Melanocortin, Type 4 / Q000235:genetics; D056564:Sirtuin 1 / Q000235:genetics
chemical_list: C470072:MC4R protein, human; D011506:Proteins; D044105:Receptor, Melanocortin, Type 4; D000071516:Alpha-Ketoglutarate-Dependent Dioxygenase FTO; C526492:FTO protein, human; C447939:SIRT1 protein, human; D056564:Sirtuin 1
keywords: FTO; MC4R; Morbid obesity; SIRT1; SNPs; metabolic syndrome