title: Modulation of keratin 1, 10 and involucrin expression as part of the complex response of the human keratinocyte cell line HaCaT to ultraviolet radiation.
abstract: Skin exposure to ultraviolet (UV) light evokes a complex stress response in keratinocytes. Keratin filament organization provides structural stability and mechanical integrity of keratinocytes. Involucrin is a transglutaminase substrate protein contributing to the formation of insoluble cornified envelopes. However, a more complex role for keratins and involucrin has been proposed, including the regulation of cell stress response. The aim was to evaluate modulations of keratin 1, 10 and involucrin expression in HaCaT in the light of the complex response of these cells to UV-B radiation, including effects on c-Jun and matrix metalloproteinase 1 (MMP-1) gene expression and production of interleukin (IL) 6 and 8. A UV-B (300±5 nm) dose of 10 mJ/cm(2) was selected since this dose resulted in a partial decrease in cell viability in contrast to higher UV-B doses, which induced complete cell death 48 h after treatment. The UV-B radiation induced significant expression of keratin 1 and 10 and decreased expression of involucrin. This was accompanied by increased expression of c-Jun and MMP-1 and IL-6 and IL-8 production. The data suggest that the expression of keratin 1, 10 and involucrin is modulated in HaCaT keratinocytes as a part of the complex stress response to UV radiation.
authors.affiliation: Contipro Biotech s.r.o., Dolní Dobrouč, Czech Republic ; Institute of Experimental Biology, Department of Physiology and Immunology of Animals, Faculty of Science, Masaryk University, Brno, Czech Republic.
authors.forename: Martina
authors.initials: M
authors.lastname: Moravcová
authors.affiliation: Generi Biotech, Hradec Kralove, Czech Republic.
authors.forename: Antonín
authors.initials: A
authors.lastname: Libra
authors.affiliation: Contipro Biotech s.r.o., Dolní Dobrouč, Czech Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Dvořáková
authors.affiliation: Generi Biotech, Hradec Kralove, Czech Republic.
authors.forename: Alena
authors.initials: A
authors.lastname: Víšková
authors.affiliation: Contipro Biotech s.r.o., Dolní Dobrouč, Czech Republic.
authors.forename: Tomáš
authors.initials: T
authors.lastname: Muthný
authors.affiliation: Contipro Biotech s.r.o., Dolní Dobrouč, Czech Republic.
authors.forename: Vladimír
authors.initials: V
authors.lastname: Velebný
authors.affiliation: Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno, Czech Republic ; International Clinical Research Center - Center of Biomolecular and Cell Engineering, St. Anne's University Hospital Brno, Brno, Czech Republic.
authors.forename: Lukáš
authors.initials: L
authors.lastname: Kubala
keywords: inflammation; involucrin; keratin; keratinocyte; ultraviolet light