title: Effects of green tea compound epigallocatechin-3-gallate against Stenotrophomonas maltophilia infection and biofilm.
abstract: We investigated the in vitro and in vivo activities of epigallocatechin-3-gallate (EGCg), a green tea component, against Stenotrophomonas maltophilia (Sm) isolates from cystic fibrosis (CF) patients. In vitro effects of EGCg and the antibiotic colistin (COL) on growth inhibition, survival, and also against young and mature biofilms of S. maltophilia were determined. Qualitative and quantitative changes on the biofilms were assessed by confocal laser scanning microscopy (CLSM). Further, in vivo effects of nebulized EGCg in C57BL/6 and Cftr mutant mice during acute Sm lung infection were evaluated. Subinhibitory concentrations of EGCg significantly reduced not only biofilm formation, but also the quantity of viable cells in young and mature biofilms. CLSM showed that EGCg-exposed biofilms exhibited either a change in total biofilm biovolume or an increase of the fraction of dead cells contained within the biofilm in a dose depended manner. Sm infected wild-type and Cftr mutant mice treated with 1,024 mg/L EGCg by inhalation exhibited significantly lower bacterial counts than those undergoing no treatment or treated with COL. EGCg displayed promising inhibitory and anti-biofilm properties against CF Sm isolates in vitro and significantly reduced Sm bacterial counts in an acute infection model with wild type and CF mice. This natural compound may represent a novel therapeutic agent against Sm infection in CF.
authors.affiliation: Institute of Medical Microbiology, University Hospital Essen, University of Duisburg-Essen, Essen, Germany.
authors.forename: Pedrina G
authors.initials: PG
authors.lastname: Vidigal
authors.affiliation: Institute for Molecular Bacteriology, TWINCORE, Center for Experimental and Clinical Infection Research, Hannover, Germany; Department of Molecular Bacteriology, Helmholtz Center for Infection Research, Braunschweig, Germany.
authors.forename: Mathias
authors.initials: M
authors.lastname: Müsken
authors.affiliation: Institute of Molecular Biology, University of Duisburg-Essen, Essen, Germany.
authors.forename: Katrin A
authors.initials: KA
authors.lastname: Becker
authors.affiliation: Institute for Molecular Bacteriology, TWINCORE, Center for Experimental and Clinical Infection Research, Hannover, Germany; Department of Molecular Bacteriology, Helmholtz Center for Infection Research, Braunschweig, Germany.
authors.forename: Susanne
authors.initials: S
authors.lastname: Häussler
authors.affiliation: Biofilm Center, Department of Aquatic Microbiology, Faculty of Chemistry, University of Duisburg-Essen, Essen, Germany.
authors.forename: Jost
authors.initials: J
authors.lastname: Wingender
authors.affiliation: Division of Experimental Virology, TWINCORE, Center for Experimental and Clinical Infection Research, Hannover, Germany.
authors.forename: Eike
authors.initials: E
authors.lastname: Steinmann
authors.affiliation: Institute of Medical Microbiology, University Hospital Essen, University of Duisburg-Essen, Essen, Germany.
authors.forename: Jan
authors.initials: J
authors.lastname: Kehrmann
authors.affiliation: Institute of Molecular Biology, University of Duisburg-Essen, Essen, Germany.
authors.forename: Erich
authors.initials: E
authors.lastname: Gulbins
authors.affiliation: Institute of Medical Microbiology, University Hospital Essen, University of Duisburg-Essen, Essen, Germany.
authors.forename: Jan
authors.initials: J
authors.lastname: Buer
authors.affiliation: Institute of Medical Microbiology, University Hospital Essen, University of Duisburg-Essen, Essen, Germany.
authors.forename: Peter Michael
authors.initials: PM
authors.lastname: Rath
authors.affiliation: Institute of Medical Microbiology, University Hospital Essen, University of Duisburg-Essen, Essen, Germany.
authors.forename: Jörg
authors.initials: J
authors.lastname: Steinmann
mesh_terms: D000818:Animals; D058491:Bacterial Load / Q000187:drug effects; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development*; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D003091:Colistin / Q000494:pharmacology; D005260:Female; D007322:Instillation, Drug; D007700:Kinetics; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D008826:Microbial Sensitivity Tests; D020615:Stenotrophomonas maltophilia / Q000187:drug effects / Q000302:isolation & purification / Q000502:physiology*; D013662:Tea / Q000737:chemistry*
chemical_list: D013662:Tea; D002392:Catechin; C045651:epigallocatechin gallate; D003091:Colistin