title: Comparision of efficacy of two experimental bovine leptospira vaccines under laboratory and field.
abstract: Two different inactivated vaccines for bovine leptospirosis were prepared with five different Leptospira serovars namely australis, ballum, hardjo, hebdomadis and pomona and adjuvanted with Montanide ISA 206 (Vaccine-I) or adjuvanted with Aluminium hydroxide gel (Vaccine-II) to evaluate the efficacy of the vaccines. The immunogenicity of the vaccines was studied in rabbits under experimental condition and in cattle under field condition for a period of 180 days. Antibody response against five different leptospira serovars ranged from 6.84 log2 to 9.64 log2 (GM-MAT titres) in rabbits at 180 days after vaccination with vaccine I, whereas in vaccine II, the titre value ranged from 5.64 log2 to 7.44 log2. In the case of cattle under field condition, vaccine I showed GM-MAT titres of 6.84 log2 to 7.69 log2 against five different serovars at 150 days following vaccination. Such titres were maintained following vaccination with vaccine II for 120 days only. It is concluded that vaccine I is a better vaccine than vaccine II. However both the vaccines showed high immune response of 5.64 log2 at six months of immunization. Vaccination did not cause any adverse reaction in the vaccinated cattle.
authors.affiliation: Department of Veterinary Microbiology, Madras Veterinary College, Tamilnadu Veterinary and Animal Sciences University, Chennai 600007, Tamil Nadu, India.
authors.forename: G
authors.initials: G
authors.lastname: Balakrishnan
authors.affiliation: Department of Veterinary Microbiology, Madras Veterinary College, Tamilnadu Veterinary and Animal Sciences University, Chennai 600007, Tamil Nadu, India. Electronic address: roy@tanuvas.org.in.
authors.forename: Parimal
authors.initials: P
authors.lastname: Roy
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000372:Agglutination Tests / Q000662:veterinary; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001428:Bacterial Vaccines / Q000276:immunology / Q000494:pharmacology*; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D005260:Female; D007919:Leptospira / Q000276:immunology*; D007922:Leptospirosis / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D011817:Rabbits; D014611:Vaccination / Q000662:veterinary*; D015164:Vaccines, Inactivated / Q000276:immunology / Q000494:pharmacology; D015047:Zoonoses / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*
chemical_list: D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D015164:Vaccines, Inactivated
keywords: Adjuvants; Bovines; Leptospira; Vaccines