title: Involvement of orexin-A neurons but not melanin-concentrating hormone neurons in the short-term regulation of food intake in rats.
abstract: In order to elucidate the involvement of melanin-concentrating hormone (MCH) and orexin-A (ORX-A) neurons of the perifornical/lateral hypothalamic areas (PF/LH) in the regulation of food intake induced by acutely reduced glucose availability, we examined the food intake response and c-Fos expression in the MCH and ORX-A neurons in the PF/LH during 2-deoxy-D-glucose (2DG)-induced glucoprivation (400 mg/kg; i.v.) and systemic insulin-induced hypoglycemia (5 U/kg; s.c.) in male Wistar rats. The administration of both 2DG and insulin stimulated food intake and induced c-Fos expression in the ORX-A neurons corresponding to food intake, but not in the MCH neurons. These data indicate that ORX-A neurons, but not MCH neurons, play a role in the short-term regulation of food intake, and that the input signals for the neurons containing MCH and ORX-A are different, and these neurons play different roles in the regulation of feeding behavior.
authors.affiliation: Department of Environmental Health, Nara Women's University, Kitauoya Nishimachi, Nara, 630-8506, Japan.
authors.forename: Yuri
authors.initials: Y
authors.lastname: Nishimura
authors.forename: Kaori
authors.initials: K
authors.lastname: Mabuchi
authors.forename: Sayumi
authors.initials: S
authors.lastname: Taguchi
authors.forename: Saori
authors.initials: S
authors.lastname: Ikeda
authors.forename: Eri
authors.initials: E
authors.lastname: Aida
authors.forename: Hiroko
authors.initials: H
authors.lastname: Negishi
authors.forename: Akira
authors.initials: A
authors.lastname: Takamata
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001833:Body Temperature Regulation; D003847:Deoxyglucose / Q000008:administration & dosage; D004195:Disease Models, Animal; D004435:Eating / Q000187:drug effects; D004734:Energy Metabolism; D005247:Feeding Behavior / Q000187:drug effects; D006943:Hyperglycemia / Q000139:chemically induced / Q000378:metabolism* / Q000523:psychology; D007028:Hypothalamic Hormones / Q000378:metabolism*; D007031:Hypothalamus / Q000187:drug effects / Q000378:metabolism*; D007328:Insulin; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D008297:Male; D008543:Melanins / Q000378:metabolism*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D009479:Neuropeptides / Q000378:metabolism*; D000068797:Orexins; D010907:Pituitary Hormones / Q000378:metabolism*; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D017208:Rats, Wistar; D013997:Time Factors
chemical_list: D001786:Blood Glucose; D007028:Hypothalamic Hormones; D007328:Insulin; D047908:Intracellular Signaling Peptides and Proteins; D008543:Melanins; D009479:Neuropeptides; D000068797:Orexins; D010907:Pituitary Hormones; D016760:Proto-Oncogene Proteins c-fos; C019463:melanin-concentrating hormone; D003847:Deoxyglucose