title: Risk factors that affect reproductive target achievement in fertile dairy cows.
abstract: The aims of the present study were to investigate (1) the risk factors that influence the achievement of reproductive targets postpartum (pp) and (2) the key factors that influence pregnancy rate following first artificial insemination (AI) in dairy cows. Ninety-eight Holstein-Friesian pp cows were blood sampled from wk 1 to 4 pp for hematology and biochemistry. Reproductive tract health was assessed weekly by ultrasonography and vaginal mucus scoring. Body condition score (BCS), lameness score, and milk yield were assessed every 2 wk. Milk samples for progesterone assay were collected twice weekly and on d 4, 5, and 7 after AI. Risk factors associated with achieving reproductive targets depended on (1) increased metabolic activity of the liver (increased glutamate dehydrogenase at calving and increased γ-glutamyl transpeptidase in wk 4), (2) a competent immune system (increased neutrophils in wk 1; decreased α1-acid glycoprotein in wk 1, 2, and 3), (3) an endocrine system that was capable of responding by producing sufficient triiodothyronine in wk 2 and increased insulin-like growth factor I in wk 3 and 4, (4) a lower negative energy balance status (decreased nonesterified fatty acid concentration in wk 1; decreased β-hydroxybutyrate concentration in wk 2; BCS loss between calving and d 28 pp <0.5), (5) good reproductive tract health [normal uterine scan at d 45 pp; clear vaginal mucus discharge at first ovulation and at d 45 pp; resumed ovarian cyclicity by the end of the voluntary waiting period (≥ d 35 pp)], and (6) adequate diet (to ensure increased glutathione peroxidase in wk 2 and 3 and increased magnesium in wk 4). Risk factors that increased the odds of a successful first AI were previous ovulation(s) (odds ratio=3.17 per ovulation), BCS >2.5 at AI (odds ratio=3.01), and clear vaginal mucus (score=0) compared with purulent mucus (score >0) 4 d after first AI (odds ratio=2.99). In conclusion, this study identified key risk factors in the early pp period that give a higher probability of cows achieving their reproductive targets and of having a first-AI pregnancy.
authors.affiliation: School of Veterinary Medicine, University College Dublin, Belfield, Dublin 4, Ireland.
authors.forename: S P M
authors.initials: SP
authors.lastname: Aungier
authors.affiliation: School of Veterinary Medicine, University College Dublin, Belfield, Dublin 4, Ireland.
authors.forename: J F
authors.initials: JF
authors.lastname: Roche
authors.affiliation: Animal and Grassland Research and Innovation Centre, Teagasc, Mellows Campus, Athenry, Co. Galway, Ireland.
authors.forename: M G
authors.initials: MG
authors.lastname: Diskin
authors.affiliation: School of Veterinary Medicine, University College Dublin, Belfield, Dublin 4, Ireland; Conway Institute for Biomolecular and Biomedical Research, University College Dublin, Belfield, Dublin 4, Ireland. Electronic address: mark.crowe@ucd.ie.
authors.forename: M A
authors.initials: MA
authors.lastname: Crowe
mesh_terms: D020155:3-Hydroxybutyric Acid / Q000378:metabolism; D000818:Animals; D002417:Cattle; D005230:Fatty Acids, Nonesterified / Q000378:metabolism; D005260:Female; D005298:Fertility; D007315:Insemination, Artificial / Q000662:veterinary; D007328:Insulin / Q000097:blood; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D007774:Lactation; D057230:Limit of Detection; D008892:Milk / Q000378:metabolism; D010060:Ovulation; D049590:Postpartum Period / Q000378:metabolism; D011247:Pregnancy; D011374:Progesterone / Q000032:analysis; D012098:Reproduction; D012307:Risk Factors; D013974:Thyroxine / Q000097:blood; D014284:Triiodothyronine / Q000097:blood; D005723:gamma-Glutamyltransferase / Q000378:metabolism
chemical_list: D005230:Fatty Acids, Nonesterified; D007328:Insulin; D014284:Triiodothyronine; D011374:Progesterone; D007334:Insulin-Like Growth Factor I; D005723:gamma-Glutamyltransferase; D013974:Thyroxine; D020155:3-Hydroxybutyric Acid
keywords: fertility; insulin-like growth factor I; negative energy balance; thyroid function