title: Cysteine oxidation reactions catalyzed by a mononuclear non-heme iron enzyme (OvoA) in ovothiol biosynthesis.
abstract: OvoA in ovothiol biosynthesis is a mononuclear non-heme iron enzyme catalyzing the oxidative coupling between histidine and cysteine. It can also catalyze the oxidative coupling between hercynine and cysteine, yet with a different regio-selectivity. Due to the potential application of this reaction for industrial ergothioneine production, in this study, we systematically characterized OvoA by a combination of three different assays. Our studies revealed that OvoA can also catalyze the oxidation of cysteine to either cysteine sulfinic acid or cystine. Remarkably, these OvoA-catalyzed reactions can be systematically modulated by a slight modification of one of its substrates, histidine.
authors.affiliation: Department of Chemistry, Boston University , 590 Commonwealth Avenue, Boston, Massachussetts 02215, United States.
authors.forename: Heng
authors.initials: H
authors.lastname: Song
authors.forename: Ampon Sae
authors.initials: AS
authors.lastname: Her
authors.forename: Fiona
authors.initials: F
authors.lastname: Raso
authors.forename: Zhibin
authors.initials: Z
authors.lastname: Zhen
authors.forename: Yuda
authors.initials: Y
authors.lastname: Huo
authors.forename: Pinghua
authors.initials: P
authors.lastname: Liu
mesh_terms: D001622:Betaine / Q000031:analogs & derivatives / Q000378:metabolism; D002384:Catalysis; D003545:Cysteine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D050558:Cysteine Dioxygenase / Q000378:metabolism; D006418:Heme / Q000378:metabolism; D006639:Histidine / Q000031:analogs & derivatives / Q000378:metabolism; D006801:Humans; D008762:Methylhistidines / Q000378:metabolism*; D008954:Models, Biological; D015394:Molecular Structure; D020102:Mycobacterium smegmatis / Q000201:enzymology / Q000378:metabolism*; D019906:Nuclear Magnetic Resonance, Biomolecular; D010084:Oxidation-Reduction
chemical_list: D008762:Methylhistidines; C050085:ovothiol C; D001622:Betaine; D006418:Heme; D006639:Histidine; D050558:Cysteine Dioxygenase; D003545:Cysteine; C007928:hercynine; C013461:cysteine sulfinic acid