title: Polyamidoamine dendrimers as off-column binding agent and in-column pseudostationary phase for efficient and sensitive capillary electrophoretic analysis of fluoroquinolones in chicken muscles.
abstract: A capillary electrophoresis method based on polyamidoamine dendrimers has been developed for quick and sensitive detection of moxifloxacin, gatifloxacin, lomefloxacin, enoxacin, ofloxacin and pazufloxacin. Increasing buffer alkalinity led to enhanced sensitivity, whereas the polyamidoamine-fluoroquinolone interactions, i.e., in-cavity hydrogen bonding/hydrophobic interaction and electrostatic interaction, contributed to the improvement in both separation efficiency and detection sensitivity. By using 1 mM polyamidoamine generation 0.5 as binding agent in sample solution and 0.1 μM polyamidoamine generation zero as pseudostationary phase to the running buffer of 5 mM sodium tetraborate at pH 9.3, the six fluoroquinolones were baseline separated in <10 min with detection limits at ng/mL level. Coupled with liquid-liquid extraction, the proposed method was successfully applied in the determination of fluoroquinolones in chicken muscle samples.
authors.affiliation: College of Chemistry, Beijing Normal University, No. 19, Xinjiekou Wai Street, Beijing 100875, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Tian
authors.affiliation: College of Chemistry, Beijing Normal University, No. 19, Xinjiekou Wai Street, Beijing 100875, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Hu
authors.affiliation: College of Chemistry, Beijing Normal University, No. 19, Xinjiekou Wai Street, Beijing 100875, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Yang
authors.affiliation: College of Chemistry, Beijing Normal University, No. 19, Xinjiekou Wai Street, Beijing 100875, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Tang
authors.affiliation: College of Chemistry, Beijing Normal University, No. 19, Xinjiekou Wai Street, Beijing 100875, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Guo
authors.affiliation: College of Chemistry, Beijing Normal University, No. 19, Xinjiekou Wai Street, Beijing 100875, China. Electronic address: qinwd@bnu.edu.cn.
authors.forename: Weidong
authors.initials: W
authors.lastname: Qin
mesh_terms: D000818:Animals; D002645:Chickens / Q000254:growth & development*; D050091:Dendrimers / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D009132:Muscles; D011073:Polyamines / Q000737:chemistry*
chemical_list: D050091:Dendrimers; C531249:Poly(amidoamine); D011073:Polyamines
keywords: Binding agent; Capillary electrophoresis; Chicken muscle; Fluoroquinolones; Polyamidoamine; Pseudostationary phase