title: Fully motorized optical-resolution photoacoustic microscopy.
abstract: We have developed fully motorized optical-resolution photoacoustic microscopy (OR-PAM), which integrates five complementary scanning modes and simultaneously provides a high imaging speed and a wide field of view (FOV) with 2.6 μm lateral resolution. With one-dimensional (1D) motion-mode mechanical scanning, we measured the blood flow through a cross section of a blood vessel in vivo. With two-dimensional (2D) optical scanning at a laser repetition rate of 40 kHz, we achieved a 2 kHz B-scan rate over a range of 50 μm with 20 A-lines and 50 Hz volumetric-scan rate over a FOV of 50  μm × 50  μm with 400 A-lines, which enabled real-time tracking of cellular dynamics in vivo. With synchronized 1D optical and 2D mechanical hybrid scanning, we imaged a 10  mm × 8  mm FOV within three minutes, which is 20 times faster than the conventional mechanical scan in our second-generation OR-PAM. With three-dimensional mechanical contour scanning, we maintained the optimal signal-to-noise ratio and spatial resolution of OR-PAM while imaging objects with uneven surfaces, which is essential for quantitative studies.
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.forename: Chenghung
authors.initials: C
authors.lastname: Yeh
authors.forename: Song
authors.initials: S
authors.lastname: Hu
authors.forename: Lidai
authors.initials: L
authors.lastname: Wang
authors.forename: Brian T
authors.initials: BT
authors.lastname: Soetikno
authors.forename: Ruimin
authors.initials: R
authors.lastname: Chen
authors.forename: Qifa
authors.initials: Q
authors.lastname: Zhou
authors.forename: K Kirk
authors.initials: KK
authors.lastname: Shung
authors.forename: Konstantin I
authors.initials: KI
authors.lastname: Maslov
authors.forename: Lihong V
authors.initials: LV
authors.lastname: Wang
mesh_terms: D000818:Animals; D004423:Ear / Q000098:blood supply; D004867:Equipment Design; D004912:Erythrocytes / Q000166:cytology; D006454:Hemoglobins / Q000032:analysis; D051379:Mice; D008853:Microscopy / Q000295:instrumentation*; D055620:Optical Phenomena; D061088:Photoacoustic Techniques / Q000295:instrumentation*
chemical_list: D006454:Hemoglobins