title: Pilot-scale continuous recycling of growth medium for the mass culture of a halotolerant Tetraselmis sp. in raceway ponds under increasing salinity: a novel protocol for commercial microalgal biomass production.
abstract: The opportunity to recycle microalgal culture medium for further cultivation is often hampered by salinity increases from evaporation and fouling by dissolved and particulate matter. In this study, the impact of culture re-use after electro-flocculation of seawater-based medium on growth and biomass productivity of the halotolerant green algal strain Tetraselmis sp., MUR 233, was investigated in pilot-scale open raceway ponds over 5months. Despite a salinity increase from 5.5% to 12% (w/v) NaCl, Tetraselmis MUR 233 grown on naturally DOC-enriched recycled medium produced 48-160% more ash free dry weight (AFDW) biomass daily per unit pond area than when grown on non-recycled medium. A peak productivity of 37.5±3.1gAFDWm(-2)d(-1) was reached in the recycled medium upon transition from ∼14% to ∼7% NaCl. The combination of high biomass-yielding mixotrophic growth under high salinity has been proven to be a successful sustainable cultivation strategy.
authors.affiliation: School of Chemical Engineering, University of Adelaide, South Australia 5005, Australia; Muradel Pty Ltd, 55/239 Magill Rd, Maylands, South Australia 5069, Australia. Electronic address: sophie.fonsing@adelaide.edu.au.
authors.forename: S
authors.initials: S
authors.lastname: Fon Sing
authors.affiliation: Algae R&D Centre, School of Veterinary and Life Sciences, Murdoch University, Murdoch, Western Australia 6150, Australia; Muradel Pty Ltd, 55/239 Magill Rd, Maylands, South Australia 5069, Australia.
authors.forename: A
authors.initials: A
authors.lastname: Isdepsky
authors.affiliation: Algae R&D Centre, School of Veterinary and Life Sciences, Murdoch University, Murdoch, Western Australia 6150, Australia.
authors.forename: M A
authors.initials: MA
authors.lastname: Borowitzka
authors.affiliation: School of Chemical Engineering, University of Adelaide, South Australia 5005, Australia; Muradel Pty Ltd, 55/239 Magill Rd, Maylands, South Australia 5069, Australia.
authors.forename: D M
authors.initials: DM
authors.lastname: Lewis
mesh_terms: D018533:Biomass; D000460:Chlorophyta / Q000254:growth & development*; D003470:Culture Media; D046508:Culture Techniques; D005427:Flocculation; D058086:Microalgae / Q000254:growth & development*; D009930:Organic Chemicals / Q000032:analysis; D010865:Pilot Projects; D054712:Salinity; D055049:Salt Tolerance; D014867:Water / Q000032:analysis
chemical_list: D003470:Culture Media; D009930:Organic Chemicals; D014867:Water
keywords: Electro-flocculation; Marine microalgae; Mixotrophy; Recycling