title: Screening of lignocellulose-degrading superior mushroom strains and determination of their CMCase and laccase activity.
abstract: In order to screen lignocellulose-degrading superior mushroom strains ten strains of mushrooms (Lentinus edodes939, Pholiota nameko, Lentinus edodes868, Coprinus comatus, Macrolepiota procera, Auricularia auricula, Hericium erinaceus, Grifola frondosa, Pleurotus nebrodensis, and Shiraia bambusicola) were inoculated onto carboxymethylcellulose agar-Congo red plates to evaluate their ability to produce carbomethyl cellulase (CMCase). The results showed that the ratio of transparent circle to mycelium circle of Hericium erinaceus was 8.16 (P < 0.01) higher than other strains. The filter paper culture screening test showed that Hericium erinaceus and Macrolepiota procera grew well and showed extreme decomposition of the filter paper. When cultivated in guaiacol culture medium to detect their abilities to secrete laccase, Hericium erinaceus showed the highest ability with the largest reddish brown circles of 4.330 cm. CMCase activity determination indicated that Coprinus comatus and Hericium erinaceus had the ability to produce CMCase with 33.92 U/L on the 9th day and 22.58 U/L on the 10th day, respectively, while Coprinus comatus and Pleurotus nebrodensis had the ability to produce laccase with 496.67 U/L and 489.17 U/L on the 16th day and 18th day. Based on the results, Coprinus comatus might be the most promising lignocellulose-degrading strain to produce both CMCase and laccase at high levels.
authors.affiliation: The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Provincial Engineering Laboratory of Biopesticide Preparation, Zhejiang A&F University, Lin'an 311300, China.
authors.forename: Li
authors.initials: L
authors.lastname: Fen
authors.affiliation: The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Provincial Engineering Laboratory of Biopesticide Preparation, Zhejiang A&F University, Lin'an 311300, China.
authors.forename: Zhu
authors.initials: Z
authors.lastname: Xuwei
authors.affiliation: School of Agricultural and Food Science, Zhejiang A&F University, Lin'an 311300, China.
authors.forename: Li
authors.initials: L
authors.lastname: Nanyi
authors.affiliation: The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Provincial Engineering Laboratory of Biopesticide Preparation, Zhejiang A&F University, Lin'an 311300, China.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Puyu
authors.affiliation: The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Provincial Engineering Laboratory of Biopesticide Preparation, Zhejiang A&F University, Lin'an 311300, China.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Shuang
authors.affiliation: The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Provincial Engineering Laboratory of Biopesticide Preparation, Zhejiang A&F University, Lin'an 311300, China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Xue
authors.affiliation: The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Provincial Engineering Laboratory of Biopesticide Preparation, Zhejiang A&F University, Lin'an 311300, China.
authors.forename: Li
authors.initials: L
authors.lastname: Pengju
authors.affiliation: Qingyuan Forestry Bureau of Zhejiang, Qingyuan 323800, China.
authors.forename: Zhu
authors.initials: Z
authors.lastname: Qichao
authors.affiliation: The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Provincial Engineering Laboratory of Biopesticide Preparation, Zhejiang A&F University, Lin'an 311300, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Haiping
mesh_terms: D000363:Agaricales / Q000201:enzymology* / Q000254:growth & development / Q000302:isolation & purification; D002266:Carboxymethylcellulose Sodium / Q000378:metabolism; D002480:Cellulase / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism*; D042845:Laccase / Q000378:metabolism*; D008031:Lignin / Q000378:metabolism*; D013045:Species Specificity
chemical_list: D005656:Fungal Proteins; C036909:lignocellulose; D008031:Lignin; D042845:Laccase; D002480:Cellulase; C019826:carboxymethylcellulase; D002266:Carboxymethylcellulose Sodium