title: Identification and survival studies of Mycobacterium tuberculosis within Laboratory-Fermented bovine milk.
abstract: BACKGROUND
  Mycobacterium tuberculosis and Mycobacterium bovis are the classic agents causing tuberculosis (TB) in humans and animals respectively. Transmission of tuberculous bacteria to humans usually occurs by inhalation of aerosols containing droplets of tubercle bacilli or via consumption of contaminated foods and drinks, primarily milk. The practice of milk pooling, including from cows with TB of the udder, further exacerbates the situation by rendering the whole milk supply infective. The simultaneous presence of indigenous lactic acid bacteria (LAB) in Mycobacterium-contaminated milk is believed to confer protective effect when the milk is adequately fermented. This study assessed the effect of LAB on the viability of mycobacteria in inherently contaminated pool of raw milk during fermentation as a function of time.
  
  
  RESULTS
  Growth was obtained in the pooled raw milk culture, and identified to be M. tuberculosis. This M. tuberculosis growth was undetectable in the milk culture by day 7 as assessed by plating serial dilutions of the milk culture for up to 14 days.
  
  
  CONCLUSIONS
  Some LAB species appear to show inhibitory effect on tubercle bacilli. If proven by more rigorous, controlled experimental results regarding such effect, selected LAB (with proven safety and efficacy) may have potential applications as anti-mycobacterial agents.
authors.affiliation: Armauer Hansen Research Institute (AHRI), P,O, Box 1005, Addis Ababa, Ethiopia. solomon.habtemariam@aau.edu.et.
authors.forename: Solomon H
authors.initials: SH
authors.lastname: Mariam
mesh_terms: D000818:Animals; D000898:Antibiosis; D058491:Bacterial Load; D061046:Biological Control Agents; D005285:Fermentation; D006801:Humans; D007777:Lactobacillaceae / Q000254:growth & development*; D050296:Microbial Viability; D008892:Milk / Q000382:microbiology*; D009169:Mycobacterium tuberculosis / Q000254:growth & development
chemical_list: D061046:Biological Control Agents