title: Low paraoxonase 1 activity predicts mortality in surgical patients with sepsis.
abstract: BACKGROUND
  State of severe oxidative stress is encountered in sepsis. Paraoxonase 1 (PON1) protects against oxidative stress but also undergoes inactivation upon that condition. We investigated PON1 activity in surgical patients with sepsis in relation to oxidative stress status, inflammation, disease severity, and survival.
  
  
  METHODS
  Prospective observational study. Sixty-nine surgical patients with sepsis were compared to 69 age/sex matched healthy controls. PON1 paraoxonase and diazoxonase activities, selected biochemical, hematological and oxidative stress parameters were measured on admission to ICU and 24, 48, 72, and 96 hours later. Disease severity scores were calculated daily.
  
  
  RESULTS
  Septic patients had significantly lower PON1 activities compared to control group at all time points. PON1 activities had good capacity to differentiate septic patients from healthy controls. Low PON1 activities were associated with higher disease severity scores and higher risk of death. Correlation between PON1 activity and markers of inflammation failed to reach significance. Decrease in PON1 activity was correlated with an increase in reducing components in plasma.
  
  
  CONCLUSIONS
  Our study demonstrated lower PON1 activity in surgical patients with sepsis compared to healthy controls. PON1 activity also reflected severity of the disease. Low PON1 activity was associated with higher mortality of surgical patients with sepsis.
authors.affiliation: Department of Anaesthesiology, Resuscitation and Intensive Care, Clinical Hospital Center Bezanijska Kosa, Bezanijska kosa bb, 11000 Belgrade, Serbia.
authors.forename: Suzana
authors.identifier: 0000-0003-2938-9585
authors.initials: S
authors.lastname: Bojic
authors.affiliation: Faculty of Pharmacy, Vojvode Stepe 450, 11000 Belgrade, Serbia.
authors.forename: Jelena
authors.identifier: 0000-0002-6980-3069
authors.initials: J
authors.lastname: Kotur-Stevuljevic
authors.affiliation: Clinical Center of Serbia, Pasterova 2, 11000 Belgrade, Serbia ; School of Medicine, University of Belgrade, Dr. Subotica 8, 11000 Belgrade, Serbia.
authors.forename: Nevena
authors.identifier: 0000-0002-1188-4249
authors.initials: N
authors.lastname: Kalezic
authors.affiliation: Faculty of Pharmacy, Vojvode Stepe 450, 11000 Belgrade, Serbia.
authors.forename: Zorana
authors.initials: Z
authors.lastname: Jelic-Ivanovic
authors.affiliation: Faculty of Pharmacy, Vojvode Stepe 450, 11000 Belgrade, Serbia.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Stefanovic
authors.affiliation: Clinical Center of Serbia, Pasterova 2, 11000 Belgrade, Serbia ; School of Medicine, University of Belgrade, Dr. Subotica 8, 11000 Belgrade, Serbia.
authors.forename: Ivan
authors.initials: I
authors.lastname: Palibrk
authors.affiliation: Clinical Chemistry Laboratory, Clinical Hospital Center Bezanijska Kosa, Bezanijska kosa bb, 11000 Belgrade, Serbia.
authors.forename: Lidija
authors.identifier: 0000-0003-3551-1428
authors.initials: L
authors.lastname: Memon
authors.affiliation: Department of Anaesthesiology, Resuscitation and Intensive Care, Clinical Hospital Center Bezanijska Kosa, Bezanijska kosa bb, 11000 Belgrade, Serbia.
authors.forename: Zdravko
authors.initials: Z
authors.lastname: Kalaba
authors.affiliation: Clinical Center of Serbia, Pasterova 2, 11000 Belgrade, Serbia.
authors.forename: Marina
authors.initials: M
authors.lastname: Stojanovic
authors.affiliation: Clinical Center of Serbia, Pasterova 2, 11000 Belgrade, Serbia ; School of Medicine, University of Belgrade, Dr. Subotica 8, 11000 Belgrade, Serbia.
authors.forename: Sanja
authors.initials: S
authors.lastname: Simic-Ogrizovic
mesh_terms: D000368:Aged; D019540:Area Under Curve; D043303:Aryldialkylphosphatase / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D011183:Postoperative Complications / Q000097:blood / Q000201:enzymology* / Q000401:mortality; D011446:Prospective Studies; D012372:ROC Curve; D018805:Sepsis / Q000097:blood / Q000201:enzymology* / Q000401:mortality
chemical_list: D015415:Biomarkers; D043303:Aryldialkylphosphatase; C468406:PON1 protein, human