title: The inhibitory effect of salvianolic acid B on TGF-β1-induced proliferation and differentiation in lung fibroblasts.
abstract: Salvianolic acid B (Sal B), one of the major water-soluble compounds of Danshen (a popular Chinese herb), possesses many of the biological activities, such as antifibrogenic effect in liver and renal diseases. Transforming growth factor-β1 (TGF-β1) plays a central role in the development of pulmonary fibrosis by stimulating extracellular matrix (ECM) accumulation and activating fibroblasts. Here, we investigated the effects of Sal B on cell proliferation, collagen synthesis, endogenous TGF-β1 production, and α-smooth muscle actin (α-SMA, a marker of myofibroblasts) expression in human lung fibroblasts stimulated by TGF-β1 in vitro. The cell proliferation rates were analyzed by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide) assay. The expression of TGF-β1 and type I collagen at both the mRNA and protein levels was detected by reverse transcription polymerase chain reaction (RT-PCR), enzyme-linked immunosorbent assay (ELISA), and radioimmunoassay, respectively. The α-SMA expression was detected by Western blot. TGF-β1 treatment of lung fibroblasts increased cell proliferation rates, and enhanced the expression level of type I collagen, endogenous TGF-β1 production, and α-SMA expression (P < .05). The treatment with only Sal B did not affect the proliferation and differentiation of lung fibroblasts. Interestingly, Sal B was found to inhibit TGF-β1-induced cell proliferation, expression of type I collagen, endogenous TGF-β1 production, and α-SMA expression in lung fibroblasts. Moreover, the inhibitory effect of Sal B on TGF-β1-induced proliferation and differentiation in lung fibroblasts was more significant when treated with high-dose Sal B (1 μmol/L versus 10 μmol/L, P < .05). These data demonstrate that Sal B inhibits TGF-β1-induced cell proliferation and differentiation in vitro experiment.
authors.affiliation: Department of Respiratory and Intensive Care Medicine (ICU), Guangzhou Red Cross Hospital, Jinan University, Guangzhou, China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Shu-Ren
authors.initials: SR
authors.lastname: Cao
authors.forename: Rui
authors.initials: R
authors.lastname: Zhang
authors.forename: Jin-Lan
authors.initials: JL
authors.lastname: Jin
authors.forename: You-Feng
authors.initials: YF
authors.lastname: Zhu
mesh_terms: D000199:Actins / Q000378:metabolism; D001572:Benzofurans / Q000494:pharmacology / Q000627:therapeutic use*; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D024042:Collagen Type I / Q000096:biosynthesis; D015536:Down-Regulation; D004353:Drug Evaluation, Preclinical; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D008517:Phytotherapy; D011658:Pulmonary Fibrosis / Q000517:prevention & control*; D027561:Salvia miltiorrhiza; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D053630:Depsides
chemical_list: D000199:Actins; D001572:Benzofurans; D024042:Collagen Type I; D004365:Drugs, Chinese Herbal; D053773:Transforming Growth Factor beta1; C541116:ACTA2 protein, human; C508960:TGFB1 protein, human; C076944:salvianolic acid B; D053630:Depsides