title: AROS has a context-dependent effect on SIRT1.
abstract: The modulation of protein deacetylase SIRT1 has a vast therapeutic potential in treatment of several aging-associated diseases. Active regulator of SIRT1 (AROS) is a small endogenous protein which was originally reported to activate SIRT1 through a direct interaction in cancer cells. We show that the interaction between the two proteins is weak and does not alter the activity of SIRT1 in non-cancerous human cells. The results of different in vitro SIRT1 activity assays disclosed AROS as an inhibitor of SIRT1. The functional relationship between AROS and SIRT1 proved to be dependent on the biological context and experimental setting.
authors.affiliation: School of Pharmacy, University of Eastern Finland, Kuopio, Finland. Electronic address: tarja.kokkola@uef.fi.
authors.forename: Tarja
authors.initials: T
authors.lastname: Kokkola
authors.affiliation: School of Pharmacy, University of Eastern Finland, Kuopio, Finland.
authors.forename: Tiina
authors.initials: T
authors.lastname: Suuronen
authors.affiliation: School of Pharmacy, University of Eastern Finland, Kuopio, Finland.
authors.forename: Ferdinand
authors.initials: F
authors.lastname: Molnár
authors.affiliation: Institute of Biomedical Technology, University of Tampere, Tampere, Finland.
authors.forename: Juha
authors.initials: J
authors.lastname: Määttä
authors.affiliation: Department of Neurology, University of Eastern Finland, Kuopio, Finland.
authors.forename: Antero
authors.initials: A
authors.lastname: Salminen
authors.affiliation: School of Pharmacy, University of Eastern Finland, Kuopio, Finland.
authors.forename: Elina M
authors.initials: EM
authors.lastname: Jarho
authors.affiliation: School of Pharmacy, University of Eastern Finland, Kuopio, Finland.
authors.forename: Maija
authors.initials: M
authors.lastname: Lahtela-Kakkonen
mesh_terms: D000107:Acetylation; D004847:Epithelial Cells / Q000201:enzymology; D057809:HEK293 Cells; D006657:Histones / Q000737:chemistry; D006801:Humans; D009243:NAD / Q000737:chemistry; D009687:Nuclear Proteins / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D011499:Protein Processing, Post-Translational; D055213:Retinal Pigment Epithelium / Q000166:cytology; D056564:Sirtuin 1 / Q000737:chemistry / Q000378:metabolism*; D014157:Transcription Factors / Q000737:chemistry / Q000378:metabolism*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
chemical_list: D006657:Histones; D009687:Nuclear Proteins; C524448:RPS19BP1 protein, human; C495901:TP53 protein, human; D014157:Transcription Factors; D016159:Tumor Suppressor Protein p53; D009243:NAD; C447939:SIRT1 protein, human; D056564:Sirtuin 1
keywords: AROS; Protein–protein interaction; SIRT1; Sirtuin; p53