title: Critical size defect regeneration by rhPTH-collagen membrane as a new tissue engineering tool.
abstract: Recombinant Human Parathyroid Hormone (rhPTH 1-34) administration is an effective treatment to improve bone mass in osteoporosis. The aim of this study was to develop a Tissue Engeenering Tool for bone regeneration. We evaluated the efficacy of a freeze dried rhPTH membrane in calvarial critical size defect (CSD). Forty-four Wistar female rats (body weight 150 ± 50 g) with CSD (5 mm) were divided into four groups: group 1: rhPTH membrane (rhPTHm); group 2: atelocollagen membrane (Cm); group 3: rhPTH and atelocollagen I (CrhPTHm); group 4: without any treatment (CG). All samples were evaluated on the 1st, 3rd, and 6th weeks (weeks) post-surgery by soft X-ray, histological and histometric studies. Soft X-ray results showed a radiolucent image with many irregular radiopaque areas. Histologically, rhPTHm was replaced by reticular bone (7%) since 3rd week, and lamellar bone ossicles (30%) at 6th week. Cm showed bone formation like composite bone type on week 1st, 3rd, and 6th (2%, 44%, and 41%, respectively). With CrhPTHm, bone formation was observed in all periods (2.4%, 48%, and 53%), showing statistical difference with CG in the 3rd and 6th wks (p = 0.03 and 0.01). Our results demonstrated the effectiveness of a new biomaterial called CrhPTHm because its ability to regenerate calvarial CSD. Moreover, the membrane represents a new local intermittent delivery system allowing rhPTH slow release.
authors.affiliation: Oral Pathology Department, Laboratory of Experimental Pathology and Tissue Engineering, Dental School, Tucumán University, Tucumán, Argentina; Laboratory of Experimental Pathology and Tissue Engineering, PROIMI-Biotechnology (Pilot Plant for Microbial Industrial Processes and Biotechnology), CONICET, Tucumán, Argentina.
authors.forename: Liliana R
authors.initials: LR
authors.lastname: Missana
authors.forename: María V
authors.initials: MV
authors.lastname: Jammal
mesh_terms: D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects*; D003094:Collagen / Q000737:chemistry / Q000494:pharmacology; D016503:Drug Delivery Systems / Q000379:methods*; D005260:Female; D006801:Humans; D008567:Membranes, Artificial; D010012:Osteogenesis / Q000187:drug effects; D010281:Parathyroid Hormone / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D008567:Membranes, Artificial; D010281:Parathyroid Hormone; D011994:Recombinant Proteins; C059821:atelocollagen; D003094:Collagen
keywords: bone regeneration; critical size defect; grafts; rhPTH; type I atelocollagen