title: Characterization of extracellular HSV-1 virions by proteomics.
abstract: The analysis of herpes simplex virus type 1 mature extracellular virions by proteomics requires highly enriched samples to limit false positives and favor the detection of true components. The protocol described below involves the removal of highly contaminating serum proteins and purification of the virions by a series of differential and density centrifugation steps. In addition, L-particles, which are viral particles devoid of genome and capsid but present in the extracellular milieu, are depleted on Ficoll 400 gradients. As previously reported, the resulting viral particles are free of most contaminants and suitable for mass spectrometry.
authors.affiliation: Department of Pathology and Cell Biology, University of Montreal, V-541 Pavillon Roger Gaudry, 2900 boul. Édouard-Montpetit, Montreal, QC, Canada, H3C 3J7, roger.lippe@umontreal.ca.
authors.forename: Roger
authors.initials: R
authors.lastname: Lippé
mesh_terms: D018259:Herpesvirus 1, Human / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D013058:Mass Spectrometry; D008967:Molecular Biology / Q000379:methods*; D040901:Proteomics; D014764:Viral Proteins / Q000096:biosynthesis*; D014771:Virion / Q000235:genetics*
chemical_list: D014764:Viral Proteins