title: Effects of porcine circovirus type 2 and pseudorabies vaccine co-inoculation on regulatory cytokine mRNA expression in pig peripheral blood mononuclear cells.
abstract: We investigated the immune response effects of porcine circovirus type 2 (PCV2) on cells inoculated with pseudorabies attenuated vaccine (PRV). Real-time PCR was used to detect the mRNA expression levels of the regulatory cytokines IL-4, IL-10, IL-12p40, and IFN-γ in pig peripheral blood mononuclear cells, after in vitro single vaccination and co-inoculation with PCV2 and the PRV. We found that PRV causes upregulation of IL-4, IL-12p40, and IFN-γ mRNA expression, while PCV2 causes mRNA upregulation of IL-4, IL-10, and IL-12p40. Moreover, PCV2 inhibited PRV-induced upregulation of IL-4, IL-12p40, and IFN-γ mRNA expression; IFN-γ mRNA expression was significantly inhibited. We conclude that PCV2 can reduce the cellular immune response to PRV.
authors.affiliation: College of Animal Science and Technology, Beijing University of Agriculture, Changping District, Beijing, China.
authors.forename: F
authors.initials: F
authors.lastname: Gao
authors.affiliation: College of Animal Science and Technology, Beijing University of Agriculture, Changping District, Beijing, China.
authors.forename: J-L
authors.initials: JL
authors.lastname: Xie
authors.affiliation: College of Animal Science and Technology, Beijing University of Agriculture, Changping District, Beijing, China.
authors.forename: C-W
authors.initials: CW
authors.lastname: Jia
authors.affiliation: College of Animal Science and Technology, Qingdao Agricultural University, Chengyang District, Qingdao Shandong, China.
authors.forename: H-Y
authors.initials: HY
authors.lastname: Ren
authors.affiliation: College of Animal Science and Technology, Beijing University of Agriculture, Changping District, Beijing, China zhoushuanghai_b@163.com.
authors.forename: S-H
authors.initials: SH
authors.lastname: Zhou
mesh_terms: D000818:Animals; D017925:Circovirus / Q000235:genetics* / Q000276:immunology / Q000472:pathogenicity; D016207:Cytokines / Q000235:genetics / Q000276:immunology*; D005786:Gene Expression Regulation / Q000276:immunology; D006801:Humans; D007963:Leukocytes, Mononuclear; D012333:RNA, Messenger / Q000235:genetics* / Q000276:immunology; D013552:Swine / Q000821:virology; D014612:Vaccines / Q000235:genetics / Q000276:immunology*
chemical_list: D016207:Cytokines; D012333:RNA, Messenger; D014612:Vaccines