title: Embolus-carried vascular endothelial cell growth factor 165 improves angiogenesis in thromboangiitis obliterans.
abstract: We investigated neovasculization effects of embolus-carried human vascular endothelial cell growth factor 165 (VEGF165)-encoded adenovirus (Ad) vector in the hindlimbs of rats with thromboangiitis obliterans (TAO). Rats were equally divided into blank control (I), TAO model (II), embolus (III), Ad-VEGF165 intravascular treatment (IV), Ad-VEGF165 intramuscular treatment (V), and embolus-carried Ad-VEGF165 (VI) groups. After interventional treatment, the neovasculization effect of the test gene was observed using immunohistochemistry. At 1 week after administration, compared with group II, groups V and VI had significantly increased microvessel densities, but no significant difference was observed between groups V and VI. At 2 weeks, groups V and VI exhibited significantly increased microvessel densities. At 1 week after administration, compared with group II, both groups V and VI showed a significant difference in the ratio between the α-smooth muscle actin count and the muscle fiber count, whereas no significant difference was observed between them. At 2 weeks, groups V and VI also exhibited significant differences in these ratios compared with the other groups. We conclude that Ad-VEGF165 promotes neovasculization in ischemic limbs. Embolus-carried Ad- VEGF165 had the most pronounced effect.
authors.affiliation: The First Affiliated Hospital of Nanchang University, Nanchang, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Yingtan People's Hospital, Yintan, China.
authors.forename: H C
authors.initials: HC
authors.lastname: Zhou
authors.affiliation: The First Affiliated Hospital of Nanchang University, Nanchang, China lqdocchina@126.com.
authors.forename: X L
authors.initials: XL
authors.lastname: Yang
authors.affiliation: The First Affiliated Hospital of Nanchang University, Nanchang, China.
authors.forename: Y P
authors.initials: YP
authors.lastname: Zhao
authors.affiliation: The First Affiliated Hospital of Nanchang University, Nanchang, China.
authors.forename: H P
authors.initials: HP
authors.lastname: Luo
authors.affiliation: The First Affiliated Hospital of Nanchang University, Nanchang, China.
authors.forename: C W
authors.initials: CW
authors.lastname: He
authors.affiliation: The First Affiliated Hospital of Nanchang University, Nanchang, China.
authors.forename: X B
authors.initials: XB
authors.lastname: Wan
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D004617:Embolism / Q000235:genetics / Q000628:therapy; D042783:Endothelial Cells / Q000473:pathology; D005121:Extremities / Q000473:pathology; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D006801:Humans; D007511:Ischemia / Q000235:genetics / Q000628:therapy; D009389:Neovascularization, Pathologic / Q000235:genetics*; D051381:Rats; D013919:Thromboangiitis Obliterans / Q000235:genetics* / Q000473:pathology / Q000628:therapy; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis / Q000235:genetics*
chemical_list: C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A