title: A dansyl-rhodamine chemosensor for Fe(III) based on off-on FRET.
abstract: A novel fluorescent chemosensor bearing a rhodamine and a dansyl moiety was developed for highly selective detection of Fe(3+) based on fluorescence resonance energy transfer (FRET) mechanism. Binding of Fe(3+) to the chemosensor induced spirolactam ring opening in the rhodamine moiety and subsequent off-on FRET from the dansyl energy donor to the rhodamine energy acceptor due to the spectral overlap between the emission of the dansyl moiety and the absorption of the ring opened rhodamine moiety. Job's plot analysis indicated a 1:1 binding stoichiometry between the chemosensor and Fe(3+). The association constant was estimated to be 2.72×10(3) M(-1) according to the Benesi-Hildebrand method. With the feature of easy synthesis, simple structural skeleton and excellent sensing ability, the newly synthesized chemosensor provided the potential for applying as a highly selective fluorescent probe in complex samples containing various competitive metal ions and developing other metal ion chemosensors to fulfill various needs of biological and environmental field.
authors.affiliation: Key Laboratory of Natural Resources of Changbai Mountain & Functional Molecules, Ministry of Education, Yanbian University, Yanji, Jilin 133002, China.
authors.forename: Jingyu
authors.initials: J
authors.lastname: Piao
authors.affiliation: Key Laboratory of Natural Resources of Changbai Mountain & Functional Molecules, Ministry of Education, Yanbian University, Yanji, Jilin 133002, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Lv
authors.affiliation: Key Laboratory of Natural Resources of Changbai Mountain & Functional Molecules, Ministry of Education, Yanbian University, Yanji, Jilin 133002, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Natural Resources of Changbai Mountain & Functional Molecules, Ministry of Education, Yanbian University, Yanji, Jilin 133002, China.
authors.forename: Tong
authors.initials: T
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Natural Resources of Changbai Mountain & Functional Molecules, Ministry of Education, Yanbian University, Yanji, Jilin 133002, China. Electronic address: wuxue@ybu.edu.cn.
authors.forename: Xue
authors.initials: X
authors.lastname: Wu
mesh_terms: D003619:Dansyl Compounds / Q000737:chemistry*; D005290:Ferric Compounds / Q000032:analysis* / Q000737:chemistry; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D007501:Iron / Q000032:analysis* / Q000737:chemistry; D012235:Rhodamines / Q000737:chemistry*
chemical_list: D003619:Dansyl Compounds; D005290:Ferric Compounds; D012235:Rhodamines; D007501:Iron
keywords: Chemosensor; Dansyl; FRET; Fe(3+); Rhodamine