title: Multifluorescence live analysis of herpes simplex virus type-1 replication.
abstract: The possibility to label specific viral and cellular structures with live cell markers such as autofluorescent proteins has greatly contributed to our understanding of diverse steps of the virus life cycle, as it allows monitoring virus replication in a spatial and temporal fashion. Here, we describe the multi-fluorescent analysis of the multi-compartment herpes simplex virus type-1 by live-cell confocal laser scanning microscopy.
authors.affiliation: Institute of Virology, University of Zurich, Winterthurerstr. 266a, 8057, Zurich, Switzerland, mseyffert@vetvir.uzh.ch.
authors.forename: Michael
authors.initials: M
authors.lastname: Seyffert
authors.forename: Anna Paula
authors.initials: AP
authors.lastname: de Oliveira
authors.forename: Cornel
authors.initials: C
authors.lastname: Fraefel
authors.forename: Rebecca
authors.initials: R
authors.lastname: Vogel
mesh_terms: D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D018259:Herpesvirus 1, Human / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D006801:Humans; D018613:Microscopy, Confocal; D008967:Molecular Biology / Q000379:methods*; D014709:Vero Cells; D014779:Virus Replication / Q000235:genetics*