title: Development of an unmarked gene deletion system for the filamentous fungi Aspergillus niger and Talaromyces versatilis.
abstract: In this article, we present a method to delete genes in filamentous fungi that allows recycling of the selection marker and is efficient in a nonhomologous end-joining (NHEJ)-proficient strain. We exemplify the approach by deletion of the gene encoding the transcriptional regulator XlnR in the fungus Aspergillus niger. To show the efficiency and advantages of the method, we deleted 8 other genes and constructed a double mutant in this species. Moreover, we showed that the same principle also functions in a different genus of filamentous fungus (Talaromyces versatilis, basionym Penicillium funiculosum). This technique will increase the versatility of the toolboxes for genome manipulation of model and industrially relevant fungi.
authors.affiliation: School of Life Sciences, University of Nottingham, University Park, Nottingham, United Kingdom.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Delmas
authors.forename: Agustina
authors.initials: A
authors.lastname: Llanos
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Parrou
authors.forename: Matthew
authors.initials: M
authors.lastname: Kokolski
authors.forename: Steven T
authors.initials: ST
authors.lastname: Pullan
authors.forename: Lee
authors.initials: L
authors.lastname: Shunburne
authors.forename: David B
authors.initials: DB
authors.lastname: Archer
mesh_terms: D001234:Aspergillus niger / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics; D005658:Fungi / Q000235:genetics*; D017353:Gene Deletion; D055786:Gene Knockout Techniques / Q000379:methods*; D032901:Talaromyces / Q000235:genetics*; D015534:Trans-Activators / Q000235:genetics
chemical_list: D005656:Fungal Proteins; D015534:Trans-Activators; C115443:XlnR protein, Aspergillus