title: A new monoclonal antibody, 4-1a, that binds to the amino terminus of human lipoprotein lipase.
abstract: Lipoprotein lipase (LPL) has been highly conserved through vertebrate evolution, making it challenging to generate useful antibodies. Some polyclonal antibodies against LPL have turned out to be nonspecific, and the available monoclonal antibodies (Mabs) against LPL, all of which bind to LPL's carboxyl terminus, have drawbacks for some purposes. We report a new LPL-specific monoclonal antibody, Mab 4-1a, which binds to the amino terminus of LPL (residues 5-25). Mab 4-1a binds human and bovine LPL avidly; it does not inhibit LPL catalytic activity nor does it interfere with the binding of LPL to heparin. Mab 4-1a does not bind to human hepatic lipase. Mab 4-1a binds to GPIHBP1-bound LPL and does not interfere with the ability of the LPL-GPIHBP1 complex to bind triglyceride-rich lipoproteins. Mab 4-1a will be a useful reagent for both biochemists and clinical laboratories.
authors.affiliation: Division of Nutritional Science, Cornell University, Ithaca, NY 14853, USA. Electronic address: ab55@cornell.edu.
authors.forename: André
authors.initials: A
authors.lastname: Bensadoun
authors.affiliation: Division of Nutritional Science, Cornell University, Ithaca, NY 14853, USA.
authors.forename: Charlene D
authors.initials: CD
authors.lastname: Mottler
authors.affiliation: Division of Nutritional Science, Cornell University, Ithaca, NY 14853, USA.
authors.forename: Chris
authors.initials: C
authors.lastname: Pelletier
authors.affiliation: Department of Medicine, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA 90095, USA.
authors.forename: Daniel
authors.initials: D
authors.lastname: Wu
authors.affiliation: Department of Medicine, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA 90095, USA.
authors.forename: Jane J
authors.initials: JJ
authors.lastname: Seo
authors.affiliation: Department of Medicine, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA 90095, USA.
authors.forename: Calvin S
authors.initials: CS
authors.lastname: Leung
authors.affiliation: Department of Medicine, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA 90095, USA.
authors.forename: Oludotun
authors.initials: O
authors.lastname: Adeyo
authors.affiliation: Department of Medicine, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA 90095, USA.
authors.forename: Chris N
authors.initials: CN
authors.lastname: Goulbourne
authors.affiliation: Department of Medicine, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA 90095, USA.
authors.forename: Peter
authors.initials: P
authors.lastname: Gin
authors.affiliation: Department of Medicine, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA 90095, USA.
authors.forename: Loren G
authors.initials: LG
authors.lastname: Fong
authors.affiliation: Department of Medicine, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA 90095, USA; Department of Human Genetics, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA 90095, USA.
authors.forename: Stephen G
authors.initials: SG
authors.lastname: Young
authors.affiliation: Department of Medicine, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA 90095, USA. Electronic address: abeigneux@mednet.ucla.edu.
authors.forename: Anne P
authors.initials: AP
authors.lastname: Beigneux
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D000915:Antibody Affinity; D000918:Antibody Specificity; D016466:CHO Cells; D002417:Cattle; D003412:Cricetulus; D015870:Gene Expression; D006493:Heparin / Q000378:metabolism; D006801:Humans; D008049:Lipase / Q000378:metabolism; D008071:Lipoprotein Lipase / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D018110:Receptors, Lipoprotein / Q000235:genetics / Q000378:metabolism*; D014162:Transfection; D014280:Triglycerides / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; C523701:GPIHBP1 protein, human; C504173:LIPC protein, human; D018110:Receptors, Lipoprotein; D014280:Triglycerides; D006493:Heparin; D008049:Lipase; C548261:LPL protein, human; D008071:Lipoprotein Lipase
keywords: GPIHBP1; Hyperlipidemia; Lipoprotein lipase; Monoclonal antibody; Triglyceride