title: Reversal of senescence by N resupply to N-starved Arabidopsis thaliana: transcriptomic and metabolomic consequences.
abstract: Leaf senescence is a developmentally controlled process, which is additionally modulated by a number of adverse environmental conditions. Nitrogen shortage is a well-known trigger of precocious senescence in many plant species including crops, generally limiting biomass and seed yield. However, leaf senescence induced by nitrogen starvation may be reversed when nitrogen is resupplied at the onset of senescence. Here, the transcriptomic, hormonal, and global metabolic rearrangements occurring during nitrogen resupply-induced reversal of senescence in Arabidopsis thaliana were analysed. The changes induced by senescence were essentially in keeping with those previously described; however, these could, by and large, be reversed. The data thus indicate that plants undergoing senescence retain the capacity to sense and respond to the availability of nitrogen nutrition. The combined data are discussed in the context of the reversibility of the senescence programme and the evolutionary benefit afforded thereby. Future prospects for understanding and manipulating this process in both Arabidopsis and crop plants are postulated.
authors.affiliation: University of Potsdam, Institute of Biochemistry and Biology, Karl-Liebknecht-Straße 24-25, Haus 20, D-14476 Potsdam-Golm, Germany Max-Planck Institute of Molecular Plant Physiology, Plant Signalling Group, Am Muehlenberg 1, D-14476 Potsdam-Golm, Germany.
authors.forename: Salma
authors.initials: S
authors.lastname: Balazadeh
authors.affiliation: Martin-Luther-University Halle-Wittenberg, Institute of Biology, Weinbergweg 10, D-06120 Halle, Germany.
authors.forename: Jörg
authors.initials: J
authors.lastname: Schildhauer
authors.affiliation: Max-Planck Institute of Molecular Plant Physiology, Central Metabolism Group, Am Muehlenberg 1, D-14476 Potsdam-Golm, Germany Max-Planck Partner Group at the Departamento de Biologia Vegetal, Universidade Federal de Viçosa, 36570-000 Viçosa, MG, Brasil.
authors.forename: Wagner L
authors.initials: WL
authors.lastname: Araújo
authors.affiliation: Departament de Biologia Vegetal, Universitat de Barcelona, Facultat de Biologia, 08028 Barcelona, Spain.
authors.forename: Sergi
authors.initials: S
authors.lastname: Munné-Bosch
authors.affiliation: Max-Planck Institute of Molecular Plant Physiology, Central Metabolism Group, Am Muehlenberg 1, D-14476 Potsdam-Golm, Germany.
authors.forename: Alisdair R
authors.initials: AR
authors.lastname: Fernie
authors.affiliation: University of Potsdam, Institute of Biochemistry and Biology, Karl-Liebknecht-Straße 24-25, Haus 20, D-14476 Potsdam-Golm, Germany Max-Planck Institute of Molecular Plant Physiology, Plant Signalling Group, Am Muehlenberg 1, D-14476 Potsdam-Golm, Germany.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Proost
authors.affiliation: Martin-Luther-University Halle-Wittenberg, Institute of Biology, Weinbergweg 10, D-06120 Halle, Germany bmr@uni-potsdam.de klaus.humbeck@pflanzenphys.uni-halle.de.
authors.forename: Klaus
authors.initials: K
authors.lastname: Humbeck
authors.affiliation: University of Potsdam, Institute of Biochemistry and Biology, Karl-Liebknecht-Straße 24-25, Haus 20, D-14476 Potsdam-Golm, Germany Max-Planck Institute of Molecular Plant Physiology, Plant Signalling Group, Am Muehlenberg 1, D-14476 Potsdam-Golm, Germany bmr@uni-potsdam.de klaus.humbeck@pflanzenphys.uni-halle.de.
authors.forename: Bernd
authors.initials: B
authors.lastname: Mueller-Roeber
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D001483:Base Sequence; D020171:Botrytis / Q000187:drug effects / Q000502:physiology; D016000:Cluster Analysis; D003624:Darkness; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D063990:Gene Ontology; D017343:Genes, Plant; D055442:Metabolome / Q000187:drug effects / Q000235:genetics*; D008969:Molecular Sequence Data; D009584:Nitrogen / Q000172:deficiency* / Q000494:pharmacology*; D059372:Nucleotide Motifs / Q000235:genetics; D010937:Plant Growth Regulators / Q000378:metabolism; D018515:Plant Leaves / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D059467:Transcriptome / Q000235:genetics*
chemical_list: D010937:Plant Growth Regulators; D012333:RNA, Messenger; D009584:Nitrogen
keywords: Arabidopsis; gene expression; metabolomics; nitrogen limitation; senescence; transcriptome.