title: Resveratrol metabolism in a non-human primate, the grey mouse lemur (Microcebus murinus), using ultra-high-performance liquid chromatography-quadrupole time of flight.
abstract: The grey mouse lemur (Microcebus murinus) is a non-human primate used to study the ageing process. Resveratrol is a polyphenol that may increase lifespan by delaying age-associated pathologies. However, no information about resveratrol absorption and metabolism is available for this primate. Resveratrol and its metabolites were qualitatively and quantitatively analyzed in male mouse-lemur plasma (after 200 mg.kg-1 of oral resveratrol) by ultra-high performance liquid chromatography (UHPLC), coupled to a quadrupole-time-of-flight (Q-TOF) mass spectrometer used in full-scan mode. Data analyses showed, in MSE mode, an ion common to resveratrol and all its metabolites: m/z 227.072, and an ion common to dihydro-resveratrol metabolites: m/z 229.08. A semi-targeted study enabled us to identify six hydrophilic resveratrol metabolites (one diglucurono-conjugated, two monoglucurono-conjugated, one monosulfo-conjugated and two both sulfo- and glucurono-conjugated derivatives) and three hydrophilic metabolites of dihydro-resveratrol (one monoglucurono-conjugated, one monosulfo-conjugated, and one both sulfo- and glucurono-conjugated derivatives). The presence of such metabolites has been already detected in the mouse, rat, pig, and humans. Free resveratrol was measurable for several hours in mouse-lemur plasma, and its two main metabolites were trans-resveratrol-3-O-glucuronide and trans-resveratrol-3-sulfate. Free dihydro-resveratrol was not measurable whatever the time of plasma collection, while its hydrophilic metabolites were present at 24 h after intake. These data will help us interpret the effect of resveratrol in mouse lemurs and provide further information on the inter-species characteristics of resveratrol metabolism.
authors.affiliation: EA 4463, Faculté des Sciences Pharmaceutiques et Biologiques, Université Paris Descartes, Sorbonne Paris Cité, Paris, France.
authors.forename: Marie-Claude
authors.initials: MC
authors.lastname: Menet
authors.affiliation: Mécanismes Adaptatifs et Evolution, UMR 7179, Centre National de la Recherche Scientifique, Muséum National d'Histoire Naturelle, Brunoy, France.
authors.forename: Julia
authors.initials: J
authors.lastname: Marchal
authors.affiliation: Mécanismes Adaptatifs et Evolution, UMR 7179, Centre National de la Recherche Scientifique, Muséum National d'Histoire Naturelle, Brunoy, France.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Dal-Pan
authors.affiliation: EA 4463, Faculté des Sciences Pharmaceutiques et Biologiques, Université Paris Descartes, Sorbonne Paris Cité, Paris, France.
authors.forename: Méryam
authors.initials: M
authors.lastname: Taghi
authors.affiliation: EA 4466, Faculté des Sciences Pharmaceutiques et Biologiques, Université Paris Descartes, Sorbonne Paris Cité, Paris, France; Service de Biochimie, AP-HP, Hôpital Européen Georges Pompidou, Paris, France.
authors.forename: Valérie
authors.initials: V
authors.lastname: Nivet-Antoine
authors.affiliation: EA 4463, Faculté des Sciences Pharmaceutiques et Biologiques, Université Paris Descartes, Sorbonne Paris Cité, Paris, France.
authors.forename: Delphine
authors.initials: D
authors.lastname: Dargère
authors.affiliation: EA 4463, Faculté des Sciences Pharmaceutiques et Biologiques, Université Paris Descartes, Sorbonne Paris Cité, Paris, France; Service de Toxicologie biologique, AP-HP, G.H. Lariboisière - Saint Louis - Fernand Widal, Paris, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Laprévote
authors.affiliation: EA 4466, Faculté des Sciences Pharmaceutiques et Biologiques, Université Paris Descartes, Sorbonne Paris Cité, Paris, France; Service de Biochimie A, G.H. Necker - Enfants malades, AP-HP, Paris, France.
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Beaudeux
authors.affiliation: Mécanismes Adaptatifs et Evolution, UMR 7179, Centre National de la Recherche Scientifique, Muséum National d'Histoire Naturelle, Brunoy, France.
authors.forename: Fabienne
authors.initials: F
authors.lastname: Aujard
authors.affiliation: Centre de Psychiatrie et Neuroscience, UMR 894 Inserm, Faculté de Médecine, Université Paris Descartes, Paris, France.
authors.forename: Jacques
authors.initials: J
authors.lastname: Epelbaum
authors.affiliation: EA 4466, Faculté des Sciences Pharmaceutiques et Biologiques, Université Paris Descartes, Sorbonne Paris Cité, Paris, France; Service de Biochimie A, G.H. Necker - Enfants malades, AP-HP, Paris, France.
authors.forename: Charles-Henry
authors.initials: CH
authors.lastname: Cottart
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001774:Blood Chemical Analysis / Q000379:methods*; D016565:Cheirogaleidae; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D051379:Mice; D051381:Rats; D000077185:Resveratrol; D013267:Stilbenes / Q000008:administration & dosage / Q000097:blood* / Q000378:metabolism
chemical_list: D013267:Stilbenes; D000077185:Resveratrol