title: Human neutrophils produce IL-12, IL-10, PGE2 and LTB4 in response to Paracoccidioides brasiliensis. Involvement of TLR2, mannose receptor and dectin-1.
abstract: The functions of phagocytic cells against pathogens are initiated by the interaction between membrane receptors and molecular structures which compose the cell wall of these microorganisms. Thus our study aimed to identify the neutrophil receptors involved in the recognition of different strains of Paracoccidioides brasiliensis and the consequent modulation of immune response through the production of cytokines and inflammatory mediators. Neutrophils did not produce TNF-alfa in response to both strains. However, these cells produce IL-12, mainly in response to Pb 265, with participation of TLR2 and dectin-1. These cells also produce L-10, whose levels were higher for Pb 18 with involvement of TLR2 and MR and only TLR2 for Pb 265. The production of PGE2 and LTB4 was detected similarly for the two strains. For PGE2, MR and dectin-1 were involved, while in relation to LTB4, none of them. In summary, we demonstrated that neutrophils have a dynamic role during host immune response to P. brasiliensis, since in addition to their role as effector cells of innate immunity; they have the capacity to modulate innate and adaptative immune response against this fungus by producing cytokines and lipidic mediators. This modulation may be toward a pró- or anti-inflammatory pattern in a dependence of P. brasiliensis strains and PRR involved in fungus recognition by these cells.
authors.affiliation: Botucatu School of Medicine, Department of Tropical Diseases, Universidade Estadual Paulista - UNESP, Bairro: Distrito de Rubião Junior S/N, Botucatu, SP 18618-000, Brazil; Department of Microbiology and Immunology, Institute of Biosciences, Universidade Estadual Paulista - UNESP, Bairro: Distrito de Rubião Junior S/N, Botucatu, SP 18618-000, Brazil. Electronic address: helbalderramas@yahoo.com.br.
authors.forename: Helanderson A
authors.initials: HA
authors.lastname: Balderramas
authors.affiliation: Amaral Carvalho Foundation - Flow Cytometry Laboratory, Jaú, SP, Brazil.
authors.forename: Marcimara
authors.initials: M
authors.lastname: Penitenti
authors.affiliation: Botucatu School of Medicine, Department of Tropical Diseases, Universidade Estadual Paulista - UNESP, Bairro: Distrito de Rubião Junior S/N, Botucatu, SP 18618-000, Brazil; Department of Microbiology and Immunology, Institute of Biosciences, Universidade Estadual Paulista - UNESP, Bairro: Distrito de Rubião Junior S/N, Botucatu, SP 18618-000, Brazil.
authors.forename: Daniela R
authors.initials: DR
authors.lastname: Rodrigues
authors.affiliation: Botucatu School of Medicine, Department of Tropical Diseases, Universidade Estadual Paulista - UNESP, Bairro: Distrito de Rubião Junior S/N, Botucatu, SP 18618-000, Brazil; Department of Pathology, Botucatu Medical School, UNESP - Universidade Estadual Paulista, Brazil.
authors.forename: Tatiana F
authors.initials: TF
authors.lastname: Bachiega
authors.affiliation: Botucatu School of Medicine, Department of Tropical Diseases, Universidade Estadual Paulista - UNESP, Bairro: Distrito de Rubião Junior S/N, Botucatu, SP 18618-000, Brazil; Department of Pathology, Botucatu Medical School, UNESP - Universidade Estadual Paulista, Brazil.
authors.forename: Reginaldo K
authors.initials: RK
authors.lastname: Fernandes
authors.affiliation: Amaral Carvalho Foundation - Flow Cytometry Laboratory, Jaú, SP, Brazil.
authors.forename: Maura Rosane Valério
authors.initials: MR
authors.lastname: Ikoma
authors.affiliation: Department of Pathology, Botucatu Medical School, UNESP - Universidade Estadual Paulista, Brazil.
authors.forename: Luciane Alarcão
authors.initials: LA
authors.lastname: Dias-Melicio
authors.affiliation: Botucatu School of Medicine, Department of Tropical Diseases, Universidade Estadual Paulista - UNESP, Bairro: Distrito de Rubião Junior S/N, Botucatu, SP 18618-000, Brazil; Department of Microbiology and Immunology, Institute of Biosciences, Universidade Estadual Paulista - UNESP, Bairro: Distrito de Rubião Junior S/N, Botucatu, SP 18618-000, Brazil.
authors.forename: Silvio L
authors.initials: SL
authors.lastname: Oliveira
authors.affiliation: Botucatu School of Medicine, Department of Tropical Diseases, Universidade Estadual Paulista - UNESP, Bairro: Distrito de Rubião Junior S/N, Botucatu, SP 18618-000, Brazil; Department of Microbiology and Immunology, Institute of Biosciences, Universidade Estadual Paulista - UNESP, Bairro: Distrito de Rubião Junior S/N, Botucatu, SP 18618-000, Brazil.
authors.forename: Ângela M V C
authors.initials: ÂM
authors.lastname: Soares
mesh_terms: D002478:Cells, Cultured; D015232:Dinoprostone / Q000096:biosynthesis / Q000276:immunology; D006801:Humans; D016753:Interleukin-10 / Q000096:biosynthesis / Q000276:immunology; D018664:Interleukin-12 / Q000096:biosynthesis / Q000276:immunology; D053765:Interleukin-12 Subunit p35; D037181:Lectins, C-Type / Q000276:immunology*; D007975:Leukotriene B4 / Q000096:biosynthesis / Q000276:immunology; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins / Q000276:immunology*; D009504:Neutrophils / Q000276:immunology*; D010228:Paracoccidioides / Q000145:classification / Q000276:immunology*; D010229:Paracoccidioidomycosis / Q000276:immunology; D011956:Receptors, Cell Surface / Q000276:immunology*; D051195:Toll-Like Receptor 2 / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis; D000099181:Dectin-1
chemical_list: D015232:Dinoprostone; D016753:Interleukin-10; D018664:Interleukin-12; D053765:Interleukin-12 Subunit p35; D037181:Lectins, C-Type; D007975:Leukotriene B4; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D011956:Receptors, Cell Surface; D051195:Toll-Like Receptor 2; D014409:Tumor Necrosis Factor-alpha; D000099181:Dectin-1; C508609:IL10 protein, human; C508858:IL12A protein, human; C495342:TLR2 protein, human
keywords: Cell receptors; Cytokines; Eicosanoids; Neutrophils; Paracoccidioides brasiliensis