title: Titania nanotube-modified screen printed carbon electrodes enhance the sensitivity in the electrochemical detection of proteins.
abstract: The use of titania nanotubes (TiO2-NT) as the working electrode provides a substantial improvement in the electrochemical detection of proteins. A biosensor designed using this strategy provided a robust method to detect protein samples at very low concentrations (Cprotein ca 1ng/μl). Reproducible measurements on protein samples at this concentration (Ip,a of 80+1.2μA) could be achieved using a sample volume of ca 30μl. We demonstrate the feasibility of this strategy for the accurate detection of penicillin binding protein, PBP2a, a marker for methicillin resistant Staphylococcus aureus (MRSA). The selectivity and efficiency of this sensor were also validated using other diverse protein preparations such as a recombinant protein tyrosine phosphatase (PTP10D) and bovine serum albumin (BSA). This electrochemical method also presents a substantial improvement in the time taken (few minutes) when compared to conventional enzyme-linked immunosorbent assay (ELISA) protocols. It is envisaged that this sensor could substantially aid in the rapid diagnosis of bacterial infections in resource strapped environments.
authors.affiliation: Solid State and Structural Chemistry Unit, Indian Institute of Science, Bangalore 560012, India.
authors.forename: Soumit S
authors.initials: SS
authors.lastname: Mandal
authors.affiliation: Molecular Biophysics Unit, Indian Institute of Science, Bangalore 560012, India.
authors.forename: Vikas
authors.initials: V
authors.lastname: Navratna
authors.affiliation: Molecular Biophysics Unit, Indian Institute of Science, Bangalore 560012, India.
authors.forename: Pratyush
authors.initials: P
authors.lastname: Sharma
authors.affiliation: Molecular Biophysics Unit, Indian Institute of Science, Bangalore 560012, India.
authors.forename: B
authors.initials: B
authors.lastname: Gopal
authors.affiliation: Solid State and Structural Chemistry Unit, Indian Institute of Science, Bangalore 560012, India.
authors.forename: Aninda J
authors.initials: AJ
authors.lastname: Bhattacharyya
mesh_terms: D015415:Biomarkers / Q000032:analysis; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D002244:Carbon / Q000737:chemistry*; D055664:Electrochemical Techniques; D004566:Electrodes; D005240:Feasibility Studies; D055624:Methicillin-Resistant Staphylococcus aureus / Q000302:isolation & purification; D043942:Nanotubes / Q000737:chemistry*; D046915:Penicillin-Binding Proteins / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013997:Time Factors; D014025:Titanium / Q000737:chemistry*
chemical_list: D015415:Biomarkers; D046915:Penicillin-Binding Proteins; D011506:Proteins; C009495:titanium dioxide; D002244:Carbon; D014025:Titanium
keywords: Electrochemical biosensor; Methicillin resistant Staphylococcus aureus; Penicillin binding protein 2a; Titania nanotubes