title: Behavior-selective apoptotic capacity of 4-(3,4,5-Trimethoxyphenoxy) benzoic acid and its methyl derivatives on two breast cancer cell lines.
abstract: Breast cancer is one of the most common tumors in females. The therapeutic resistance of breast cancer has motivated the development of new agents for prevention and treatment. For the present study, several compounds were designed and analyzed for their antitumor activity in many cancer cell lines. 4-(3,4,5-Trimethoxyphenoxy) benzoic acid (compound 1) and its derivatives were selected for studying the anti-proliferative and cytotoxic effects on five human cancer cell lines. Results indicated that compounds 1 and 2 significantly suppressed the cell viability of MCF-7 and MDA-MB-468 cancer cells. However, compounds 1 and 2 had only minor effects on HepG2, Huh-7, and Hela cells. Moreover, compounds 1 and 2 exhibited a novel anti-tumor activity through the induction of cell-cycle arrest at G2/M and apoptosis in MCF-7 and MDA-MB-486 breast cancer cells. Both compounds reduced colony-forming ability in MCF-7 cells. Flow cytometric analysis indicated that caspase-3 activity was increased in response to treatment with compounds 1 and 2. Taken together, these findings suggest that the novel compounds 1 and 2 are potential anticancer agents with clinical promise for breast cancer therapy.
authors.affiliation: Department of Biotechnology, Chia-Nan University of Pharmacy and Science, Tainan, Taiwan, R.O.C. hung86@mail.chna.edu.tw.
authors.forename: Kuan-Han
authors.initials: KH
authors.lastname: Lee
authors.forename: Wen-Yueh
authors.initials: WY
authors.lastname: Ho
authors.forename: Shu-Jing
authors.initials: SJ
authors.lastname: Wu
authors.forename: Tsung-Lin
authors.initials: TL
authors.lastname: Cheng
authors.forename: Po-Jui
authors.initials: PJ
authors.lastname: Huang
authors.forename: Clay C C
authors.initials: CC
authors.lastname: Wang
authors.forename: Jui-Hsiang
authors.initials: JH
authors.lastname: Hung
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D019817:Benzoic Acid / Q000737:chemistry / Q000494:pharmacology*; D001943:Breast Neoplasms; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D006801:Humans; D020128:Inhibitory Concentration 50; D061986:MCF-7 Cells; D014410:Tumor Stem Cell Assay
chemical_list: D000970:Antineoplastic Agents; D019817:Benzoic Acid
keywords: MCF-7; MDA-468; apoptosis; breast cancer; caspase-3; colony formation