title: Bone marrow-derived mononuclear cell transplantation in spinal cord injury patients by lumbar puncture.
abstract: OBJECTIVE
  This study was conducted to assess the safety and feasibility of intrathecal transplantation of autologous bone marrow-derived mononuclear cells for the treatment of patients with spinal cord injury.
  
  
  METHODS
  Ten patients were included in the study. Approximately 120 ml of bone marrow aspirate was obtained from bilateral iliac bone of patients with spinal cord injury. Isolation of mononuclear cells was performed using Ficoll density-gradient centrifugation. Bone marrow mononuclear cells were transplanted into cerebrospinal fluid by lumbar puncture. Functional tests were performed prior to the cell transplantation and six months after cell transplantation. The patients were carefully observed for up to six months.
  
  
  RESULTS
  In 5 patients with AIS A prior to cell transplantation, 1 patient converted to AIS B six months after cell transplantation. In 5 patients with AIS B, 1 patient converted to AIS D and 2 patients to AIS C. MRI did not show any complication. Two patients showed slight anemia after aspiration of bone-marrow cells, which returned to normal level within a several weeks.
  
  
  CONCLUSIONS
  The results of this study suggest that this method may be safe and feasible.
authors.affiliation: Department of Plastic and Reconstructive Surgery, Kitano Hospital, Tazuke Kofukai Medical Research Institute, Osaka, Japan.
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Suzuki
authors.affiliation: Department of Plastic and Reconstructive Surgery, Kitano Hospital, Tazuke Kofukai Medical Research Institute, Osaka, Japan.
authors.forename: Namiko
authors.initials: N
authors.lastname: Ishikawa
authors.affiliation: Translational Research Informatics Center, Kobe, Japan.
authors.forename: Kaoru
authors.initials: K
authors.lastname: Omae
authors.affiliation: Department of Plastic and Reconstructive Surgery, Kitano Hospital, Tazuke Kofukai Medical Research Institute, Osaka, Japan.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Hirai
authors.affiliation: Ikesucho-clinic, Kyoto, Japan.
authors.forename: Katsunori
authors.initials: K
authors.lastname: Ohnishi
authors.affiliation: Institute of Regeneration and Rehabilitation, Faculty of Nursing and Rehabilitation, Aino University, Osaka, Japan.
authors.forename: Norihiko
authors.initials: N
authors.lastname: Nakano
authors.affiliation: Nakayama Veterinary Hospital, Nara, Japan.
authors.forename: Hidetaka
authors.initials: H
authors.lastname: Nishida
authors.affiliation: Emergency and Critical Care Center, Kansai Medical University, Moriguchi, Osaka, Japan.
authors.forename: Toshio
authors.initials: T
authors.lastname: Nakatani
authors.affiliation: Translational Research Informatics Center, Kobe, Japan.
authors.forename: Masanori
authors.initials: M
authors.lastname: Fukushima
authors.affiliation: Institute of Regeneration and Rehabilitation, Faculty of Nursing and Rehabilitation, Aino University, Osaka, Japan.
authors.forename: Chizuka
authors.initials: C
authors.lastname: Ide
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000378:metabolism; D016026:Bone Marrow Transplantation / Q000009:adverse effects; D005500:Follow-Up Studies; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000637:transplantation; D008297:Male; D008875:Middle Aged; D020127:Recovery of Function; D013119:Spinal Cord Injuries / Q000601:surgery*; D013129:Spinal Puncture / Q000009:adverse effects; D014182:Transplantation, Autologous; D016896:Treatment Outcome; D055815:Young Adult
chemical_list: D015703:Antigens, CD
keywords: Central nervous system regeneration; bone marrow mononuclear cell; cell transplantation; clinical study; spinal cord injury treatment