title: The PDZ-binding domain of syndecan-2 inhibits LFA-1 high-affinity conformation.
abstract: Syndecans are cell membrane proteoglycans that can modulate the activity and dynamics of some growth factor receptors and integrins. Here, we show the down-regulation of integrin lymphocyte function-associated antigen-1 (LFA-1) and inhibition of adhesion of Jurkat T cells transfected with syndecan-2. The PDZ-binding domain in the cytoplasmic region of syndecan-2 was necessary to block the LFA-1 high-affinity conformation, and to reduce cellular adhesion. A second cytoplasmic motif comprising tyrosines 179 and 191, and serines 187 and 188 contributed also to reduce LFA-1 function and cellular adhesion. Inhibition of the LFA-1 high-affinity conformation by syndecan-2 was independent of the expression of the talin head domain and RhoA, Rac1 and Cdc42 GTPases. These results demonstrate the importance of PDZ-binding domain of syndecan-2 for controlling LFA-1 affinity and cell adhesion.
authors.affiliation: Celltec-UB (Department of Cell Biology), Facultat de Biologia, Universitat de Barcelona, Av. Diagonal 643, 08028 Barcelona, Spain. Electronic address: x.rovira.c@gmail.com.
authors.forename: Xavier
authors.initials: X
authors.lastname: Rovira-Clavé
authors.affiliation: Celltec-UB (Department of Cell Biology), Facultat de Biologia, Universitat de Barcelona, Av. Diagonal 643, 08028 Barcelona, Spain. Electronic address: maria.angulib@gmail.com.
authors.forename: Maria
authors.initials: M
authors.lastname: Angulo-Ibáñez
authors.affiliation: Celltec-UB (Department of Cell Biology), Facultat de Biologia, Universitat de Barcelona, Av. Diagonal 643, 08028 Barcelona, Spain. Electronic address: mreina@ub.edu.
authors.forename: Manuel
authors.initials: M
authors.lastname: Reina
authors.affiliation: Celltec-UB (Department of Cell Biology), Facultat de Biologia, Universitat de Barcelona, Av. Diagonal 643, 08028 Barcelona, Spain; Department of Physiology and Immunology, Facultat de Biologia, Universitat de Barcelona, Av. Diagonal 643, 08028 Barcelona, Spain. Electronic address: eespel@ub.edu.
authors.forename: Enric
authors.initials: E
authors.lastname: Espel
mesh_terms: D001402:B-Lymphocytes / Q000378:metabolism; D002448:Cell Adhesion / Q000502:physiology*; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism; D002465:Cell Movement; D015536:Down-Regulation; D004727:Endothelium / Q000166:cytology / Q000378:metabolism; D020690:GTPase-Activating Proteins / Q000096:biosynthesis; D006801:Humans; D019169:Jurkat Cells; D016169:Lymphocyte Function-Associated Antigen-1 / Q000096:biosynthesis* / Q000378:metabolism; D054731:PDZ Domains / Q000235:genetics*; D010750:Phosphoproteins / Q000096:biosynthesis; D011485:Protein Binding; D053669:Syndecan-2 / Q000235:genetics / Q000378:metabolism*; D013601:T-Lymphocytes / Q000378:metabolism; D016608:Talin / Q000096:biosynthesis; D014162:Transfection; D020830:rac1 GTP-Binding Protein / Q000096:biosynthesis; D020742:rhoA GTP-Binding Protein / Q000096:biosynthesis
chemical_list: C514543:ARHGAP31 protein, human; D020690:GTPase-Activating Proteins; D016169:Lymphocyte Function-Associated Antigen-1; D010750:Phosphoproteins; C107953:RAC1 protein, human; C508245:SDC2 protein, human; D016608:Talin; D053669:Syndecan-2; D020830:rac1 GTP-Binding Protein; D020742:rhoA GTP-Binding Protein
keywords: Cell adhesion; Integrin; Proteoglycan; Talin