title: Expression, purification and antibody preparation using different constructs of PCV2 capsid protein.
abstract: Capsid protein (Cap) of porcine circovirus 2 (PCV2) contained critical epitopes for inducing a protective immune response. Here, different fragments of PCV2 Cap protein were cloned, expressed, purified and used to raise polyclonal antibodies. The result showed the recombinant plasmids expressed efficiently in the prokaryotic system. Western blot and ELISA showed the recombinant protein had antigenicity and immunogenicity. Furthermore, efficiency of different constructs to produce antibody against PCV2 was compared. Reactivity and specificity of the polyclonal antibody were characterized by Western blot and indirect immunofluorescent assays. The results indicated that polyclonal antiserum prepared from protein ΔCap17-233 had better reactivity and specificity against PCV2 in comparison to that of protein ΔCap51-233 and the inactivated vaccine. These results will contribute to further studies focusing on the gene and vaccine development against PCV2.
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, 5333 Xi'an Road, Changchun, Jilin 130062, China.
authors.forename: Fuwang
authors.initials: F
authors.lastname: Chen
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, 5333 Xi'an Road, Changchun, Jilin 130062, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Yang
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, 5333 Xi'an Road, Changchun, Jilin 130062, China.
authors.forename: Daxing
authors.initials: D
authors.lastname: Pang
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, 5333 Xi'an Road, Changchun, Jilin 130062, China.
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Peng
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, 5333 Xi'an Road, Changchun, Jilin 130062, China.
authors.forename: Meichen
authors.initials: M
authors.lastname: Dong
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, 5333 Xi'an Road, Changchun, Jilin 130062, China.
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Liu
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, 5333 Xi'an Road, Changchun, Jilin 130062, China.
authors.forename: Hongsheng
authors.initials: H
authors.lastname: Ouyang
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, 5333 Xi'an Road, Changchun, Jilin 130062, China. Electronic address: renlz@jlu.edu.cn.
authors.forename: Linzhu
authors.initials: L
authors.lastname: Ren
mesh_terms: D000818:Animals; D036022:Capsid Proteins / Q000096:biosynthesis* / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D018173:Circoviridae Infections / Q000235:genetics / Q000821:virology; D017925:Circovirus / Q000235:genetics* / Q000276:immunology; D000939:Epitopes / Q000235:genetics; D015967:Gene Expression Regulation, Viral / Q000235:genetics; D013552:Swine / Q000821:virology
chemical_list: D036022:Capsid Proteins; D000939:Epitopes
keywords: Antibody; Capsid protein (Cap protein); Porcine circovirus 2