title: Structural investigation and immunological activity of a heteropolysaccharide from Sargassum fusiforme.
abstract: A heteropolysaccharide was isolated from the brown alga, Sargassum fusiforme. The heteropolysaccharide was estimated to have a molecular weight of 11kDa and showed [α]D(20) -62.2420 (c 0.05, H2O). SFPS65-B comprised galactose, glucose, mannose, fucose, and galacturonic acid at a ratio of 3.04:1:1.15:2.82:6.51. Its structural features were investigated using composition analysis, methylation analysis, IR, NMR spectroscopy, and ESI-Q-TOF MS spectroscopy. Results showed that SFPS65-B contained the backbone of →4)-α-GalAp-(1→4)-α-Hexp-(1→4)-α-GalAp-(1→4)-α-Fucp-(1→4)-α-GalAp-(1→. The sulfated unit and terminal fucose residues were attached onto the backbone through the O-2 of some galactose residues. Results also showed that SFPS65-B had a good effect on thymus and spleen indices at a dose of 100mg/kg upon immunosuppression in cyclophosphamide-treated mice.
authors.affiliation: Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 501 Haike Rd., Zhangjiang, Pudong, Shanghai 201203, PR China; Department of Clinical Pharmacology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, 528 Zhangheng Rd., Zhangjiang, Pudong, Shanghai 201203, PR China.
authors.forename: Pei
authors.initials: P
authors.lastname: Hu
authors.affiliation: Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 501 Haike Rd., Zhangjiang, Pudong, Shanghai 201203, PR China.
authors.forename: Rui
authors.initials: R
authors.lastname: Xue
authors.affiliation: Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 501 Haike Rd., Zhangjiang, Pudong, Shanghai 201203, PR China.
authors.forename: Zhixiong
authors.initials: Z
authors.lastname: Li
authors.affiliation: Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 501 Haike Rd., Zhangjiang, Pudong, Shanghai 201203, PR China.
authors.forename: Mingcang
authors.initials: M
authors.lastname: Chen
authors.affiliation: Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 501 Haike Rd., Zhangjiang, Pudong, Shanghai 201203, PR China.
authors.forename: Zhaolin
authors.initials: Z
authors.lastname: Sun
authors.affiliation: Department of Clinical Pharmacology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, 528 Zhangheng Rd., Zhangjiang, Pudong, Shanghai 201203, PR China. Electronic address: jianjiangsg@126.com.
authors.forename: Jian
authors.initials: J
authors.lastname: Jiang
authors.affiliation: Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 501 Haike Rd., Zhangjiang, Pudong, Shanghai 201203, PR China. Electronic address: cghsimm@126.com.
authors.forename: Chenggang
authors.initials: C
authors.lastname: Huang
mesh_terms: D000276:Adjuvants, Immunologic / Q000737:chemistry* / Q000494:pharmacology; D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D003520:Cyclophosphamide / Q000494:pharmacology; D007166:Immunosuppressive Agents / Q000494:pharmacology; D008813:Mice, Inbred ICR; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology; D044647:Sargassum / Q000737:chemistry*; D013154:Spleen / Q000187:drug effects / Q000276:immunology; D013950:Thymus Gland / Q000187:drug effects / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D007166:Immunosuppressive Agents; D011134:Polysaccharides; D003520:Cyclophosphamide
keywords: Chemical structure; ESI-QTOF-MS; Heteropolysaccharide; Sargassum fusiforme