title: Immature articular cartilage and subchondral bone covered by menisci are potentially susceptive to mechanical load.
abstract: BACKGROUND
  The differences of mechanical and histological properties between cartilage covered by menisci and uncovered by menisci may contribute to the osteoarthritis after meniscectomy and these differences are not fully understood. The purpose of this study is to investigate potential differences in the mechanical and histological properties, and in particular the collagen architecture, of the superficial cartilage layer and subchondral bone between regions covered and uncovered by menisci using immature knee.
  
  
  METHODS
  Osteochondral plugs were obtained from porcine tibial cartilage that was either covered or uncovered by menisci. Investigation of the thickness, mechanical properties, histology, and water content of the cartilage as well as micro-computed tomography analysis of the subchondral bone was performed to compare these regions. Collagen architecture was also assessed by using scanning electron microscopy.
  
  
  RESULTS
  Compared to the cartilage uncovered by menisci, that covered by menisci was thinner and showed a higher deformity to compression loading and higher water content. In the superficial layer of cartilage in the uncovered regions, collagen fibers showed high density, whereas they showed low density in covered regions. Furthermore, subchondral bone architecture varied between the 2 regions, and showed low bone density in covered regions.
  
  
  CONCLUSIONS
  Cartilage covered by menisci differed from that uncovered in both its mechanical and histological properties, especially with regards to the density of the superficial collagen layer. These regional differences may be related to local mechanical environment in normal condition and indicate that cartilage covered by menisci is tightly guarded by menisci from extreme mechanical loading. Our results indicate that immature cartilage degeneration and subchondral microfracture may occur easily to extreme direct mechanical loading in covered region after meniscectomy.
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Iijima
authors.forename: Tomoki
authors.initials: T
authors.lastname: Aoyama
authors.forename: Akira
authors.initials: A
authors.lastname: Ito
authors.forename: Junichi
authors.initials: J
authors.lastname: Tajino
authors.forename: Momoko
authors.initials: M
authors.lastname: Nagai
authors.forename: Xiangkai
authors.initials: X
authors.lastname: Zhang
authors.forename: Shoki
authors.initials: S
authors.lastname: Yamaguchi
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Akiyama
authors.affiliation: Department of Motor Function Analysis, Human Health Sciences, Graduate School of Medicine, Kyoto University, 53 Shogoin, Sakyo-ku, Kyoto 606-8507, Japan. kuroki.hiroshi.6s@kyoto-u.ac.jp.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kuroki
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D001834:Body Water; D002358:Cartilage, Articular / Q000737:chemistry / Q000254:growth & development / Q000502:physiology*; D003094:Collagen / Q000648:ultrastructure; D004198:Disease Susceptibility; D008592:Menisci, Tibial / Q000254:growth & development / Q000502:physiology* / Q000601:surgery; D008855:Microscopy, Electron, Scanning; D020370:Osteoarthritis, Knee / Q000209:etiology; D011509:Proteoglycans / Q000032:analysis; D013552:Swine; D013977:Tibia / Q000033:anatomy & histology* / Q000737:chemistry / Q000254:growth & development / Q000648:ultrastructure; D016474:Weight-Bearing; D055114:X-Ray Microtomography
chemical_list: D011509:Proteoglycans; D003094:Collagen