title: Detection and quantification of the Bcr/Abl chimeric protein on biochips using LDI-TOF MS.
abstract: The Bcr/Abl chimeric protein was captured by two antibodies, anti-Bcr on gold nanoparticles (AuNPs) and anti-Abl on a biochip, in a sandwich assay format. The presence of the Bcr/Abl in cells was then verified by amplified LDI-TOF MS signals, and relative amounts were quantified using AuNPs coated with deuterated alkanethiols as an internal standard.
authors.affiliation: Department of Bioscience and Biotechnology, Bio/Molecular Informatics Center, Konkuk University, Seoul 143-701, Korea. wsyeo@konkuk.ac.kr.
authors.forename: Seol-Hye
authors.initials: SH
authors.lastname: Hong
authors.forename: Jae Il
authors.initials: JI
authors.lastname: Kim
authors.forename: Hyunook
authors.initials: H
authors.lastname: Kang
authors.forename: Soojin
authors.initials: S
authors.lastname: Yoon
authors.forename: Dong-Eun
authors.initials: DE
authors.lastname: Kim
authors.forename: Woong
authors.initials: W
authors.lastname: Jung
authors.forename: Woon-Seok
authors.initials: WS
authors.lastname: Yeo
mesh_terms: D000818:Animals; D002460:Cell Line; D051379:Mice; D016374:Oncogene Proteins v-abl / Q000032:analysis*; D015514:Oncogene Proteins, Fusion / Q000032:analysis*; D040081:Protein Array Analysis / Q000379:methods*; D051562:Proto-Oncogene Proteins c-bcr / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D016374:Oncogene Proteins v-abl; D015514:Oncogene Proteins, Fusion; D051562:Proto-Oncogene Proteins c-bcr