title: Loading of nuclear autoantigens prototypically recognized by systemic lupus erythematosus sera into late apoptotic vesicles requires intact microtubules and myosin light chain kinase activity.
abstract: Most cases of systemic lupus erythematosus (SLE) are characterized by an impaired clearance of apoptotic cells in various tissues. Non-cleared apoptotic waste is considered an immunogen driving the autoimmune response in patients with SLE. During the execution of apoptosis, membrane blebs are formed and filled with cellular components. Here, we evaluate the cytoskeletal pathway(s) responsible for the loading of SLE prototypic nuclear autoantigens into the apoptotic cell-derived membranous vesicles (ACMV) generated during late phases of apoptosis. HeLa cells expressing a fusion protein of histone H2B with green fluorescent protein (GFP) were irradiated with ultraviolet (UV)-B to induce apoptosis. The appearance and trafficking of chromatin-derived material was monitored by fluorescence microscopy. Specific inhibitors of cytoskeletal pathways were employed to identify the motile elements involved in translocation and trafficking of the nuclear components. We observed that immediately after their appearance the ACMV did not contain histone H2B(GFP) ; in this phase the fluorescence was contained in the nuclear remnants and the cytoplasm. Within consecutive minutes the ACMV were loaded with chromatin-derived material, whereas the loading of simultaneously created ACMV with histone H2B(GFP) was not uniform. Some ACMV were preferentially filled and, consequently, showed a remarkably higher histone H2B(GFP) accumulation. Inhibitors of the cytoskeleton revealed that functional microtubules and myosin light chain kinase are required for nuclear shrinkage and loading of nuclear material into the ACMV, respectively.
authors.affiliation: Department of Internal Medicine 3, University of Erlangen-Nürnberg, Erlangen, Germany; Department for General Pediatrics, Haematology and Oncology, University Children's Hospital Tübingen, Tübingen, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Zirngibl
authors.forename: B G
authors.initials: BG
authors.lastname: Fürnrohr
authors.forename: C
authors.initials: C
authors.lastname: Janko
authors.forename: L E
authors.initials: LE
authors.lastname: Munoz
authors.forename: R E
authors.initials: RE
authors.lastname: Voll
authors.forename: C D
authors.initials: CD
authors.lastname: Gregory
authors.forename: G
authors.initials: G
authors.lastname: Schett
authors.forename: M
authors.initials: M
authors.lastname: Herrmann
mesh_terms: D017209:Apoptosis / Q000276:immunology* / Q000528:radiation effects; D001324:Autoantigens / Q000276:immunology*; D002460:Cell Line; D053145:Cell Nucleus Size; D055252:Cell-Derived Microparticles / Q000276:immunology* / Q000378:metabolism*; D003599:Cytoskeleton / Q000378:metabolism; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000276:immunology* / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism*; D008954:Models, Biological; D009219:Myosin-Light-Chain Kinase / Q000378:metabolism*
chemical_list: D001324:Autoantigens; D009219:Myosin-Light-Chain Kinase
keywords: apoptosis; blebbing; nuclear autoantigens; systemic lupus erythematosus