title: Structural characterization of the polysaccharide moiety of an aqueous glycopeptide from mannatide.
abstract: A homogeneous glycopeptide with a molecular weight of 1.61×10(5)Da, termed as MT2-A, was isolated from crude mannatide by DEAE-52 cellulose column and Sephacryl S-300 gel column. The polysaccharide moiety of MT2-A was mainly composed of mannose and trace amount of glucose. Besides, MT2-A contained 16 kinds of amino acids and the total amino acid content was 4.41%. The detailed chemical structure of MT2-A was elucidated using methylation analysis, Fourier transform infrared spectroscopy (FT-IR), partial acid hydrolysis, selective acetolysis and 1D/2D nuclear magnetic resonance (NMR) spectroscopy. Based on the experimental results, it was concluded that the polysaccharide moiety of MT2-A had a backbone of (1→6)-α-D-mannopyranose residues, which highly branched at O-2 position of (1→2,6)-α-D-mannopyranose residues. The side chains were mainly composed of (1→)-α-D-mannopyranose, (1→2)-α-D-mannopyranose and (1→3)-α-D-mannopyranose residues. The length of the side chains could not be longer than four mannose residues.
authors.affiliation: Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, PR China.
authors.forename: Si
authors.initials: S
authors.lastname: Li
authors.affiliation: Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, PR China.
authors.forename: Chen
authors.initials: C
authors.lastname: Pan
authors.affiliation: Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, PR China. Electronic address: xiawei1999@ecust.edu.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Xia
authors.affiliation: Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, PR China. Electronic address: zhwqing@ecust.edu.cn.
authors.forename: Wenqing
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, PR China.
authors.forename: Shengyu
authors.initials: S
authors.lastname: Wu
mesh_terms: D005947:Glucose / Q000737:chemistry; D006020:Glycopeptides / Q000737:chemistry*; D006868:Hydrolysis; D008358:Mannose / Q000737:chemistry*; D008745:Methylation; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D006020:Glycopeptides; D011134:Polysaccharides; D005947:Glucose; D008358:Mannose
keywords: Mannatide; Polysaccharide; Structure