title: Higher LRRFIP1 expression in glioblastoma multiforme is associated with better response to teniposide, a type II topoisomerase inhibitor.
abstract: Previous studies from this laboratory indicated that microRNA-21 (miR-21) contributes to chemoresistance of glioblastoma multiforme (GBM) cells to teniposide, a type II topoisomerase inhibitor. We also showed that LRRFIP1 is a target of miR-21. In this study, we found that higher baseline LRRFIP1 expression in human GBM tissue (n=60) is associated with better prognosis upon later treatment with teniposide. Experiments in cultured U373MG cells showed enhanced toxicity of teniposide against U373MG cells transfected with a vector that resulted in LRRFIP1 overexpression (vs. cells transfected with control vector). Experiments in nude mice demonstrated better response of LRRFIP1 overexpressing xenografts to teniposide. These findings indicate that high baseline LRRFIP1 expression in GBM is associated with better response to teniposide, and encourage exploring LRRFIP1 as a target for GBM treatment.
authors.affiliation: Department of Pathology, Changzheng Hospital, Second Military Medical University, 415 Fengyang Road, Shanghai, China.
authors.forename: Wei-Qing
authors.initials: WQ
authors.lastname: Li
authors.affiliation: Department of Pathology, Changzheng Hospital, Second Military Medical University, 415 Fengyang Road, Shanghai, China.
authors.forename: Hong-Yu
authors.initials: HY
authors.lastname: Yu
authors.affiliation: Department of Neurosurgery, Changzheng Hospital, Second Military Medical University, 415 Fengyang Road, Shanghai, China. Electronic address: yiyimig@163.com.
authors.forename: Yi-Ming
authors.initials: YM
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, Changzheng Hospital, Second Military Medical University, 415 Fengyang Road, Shanghai, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Pathology, Changzheng Hospital, Second Military Medical University, 415 Fengyang Road, Shanghai, China.
authors.forename: Jin
authors.initials: J
authors.lastname: He
authors.affiliation: Department of Pathology, Changzheng Hospital, Second Military Medical University, 415 Fengyang Road, Shanghai, China.
authors.forename: Hong-Zhu
authors.initials: HZ
authors.lastname: Yan
authors.affiliation: Department of Pathology, Changzheng Hospital, Second Military Medical University, 415 Fengyang Road, Shanghai, China.
authors.forename: Dao-Hua
authors.initials: DH
authors.lastname: Yang
authors.affiliation: Department of Neurosurgery, Changzheng Hospital, Second Military Medical University, 415 Fengyang Road, Shanghai, China.
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Wu
authors.affiliation: Department of Neurosurgery, Changzheng Hospital, Second Military Medical University, 415 Fengyang Road, Shanghai, China.
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Hou
authors.affiliation: Department of Pathology, Changzheng Hospital, Second Military Medical University, 415 Fengyang Road, Shanghai, China.
authors.forename: Hui-Min
authors.initials: HM
authors.lastname: Liu
authors.affiliation: Department of Pathology, Changzheng Hospital, Second Military Medical University, 415 Fengyang Road, Shanghai, China.
authors.forename: Chun-Yan
authors.initials: CY
authors.lastname: Xia
authors.affiliation: Department of Neurosurgery, Changzheng Hospital, Second Military Medical University, 415 Fengyang Road, Shanghai, China. Electronic address: luyicheng10@gmail.com.
authors.forename: Yi-Cheng
authors.initials: YC
authors.lastname: Lu
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D001932:Brain Neoplasms / Q000175:diagnosis / Q000188:drug therapy* / Q000235:genetics; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D005909:Glioblastoma / Q000175:diagnosis / Q000188:drug therapy* / Q000235:genetics; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics; D011379:Prognosis; D016601:RNA-Binding Proteins / Q000235:genetics*; D013713:Teniposide / Q000627:therapeutic use*; D059005:Topoisomerase II Inhibitors / Q000627:therapeutic use*; D014162:Transfection; D015854:Up-Regulation
chemical_list: C114370:LRRFIP1 protein, human; C524940:MIRN21 microRNA, human; D035683:MicroRNAs; D016601:RNA-Binding Proteins; D059005:Topoisomerase II Inhibitors; D013713:Teniposide
keywords: Glioblastoma multiforme; LRRFIP1; Teniposide; VM-26