title: Impact of inhaled nitric oxide on the sulfatide profile of neonatal rat brain studied by TOF-SIMS imaging.
abstract: Despite advances in neonatal intensive care leading to an increased survival rate in preterm infants, brain lesions and subsequent neurological handicaps following preterm birth remain a critical issue. To prevent brain injury and/or enhance repair, one of the most promising therapies investigated in preclinical models is inhaled nitric oxide (iNO). We have assessed the effect of this therapy on brain lipid content in air- and iNO-exposed rat pups by mass spectrometry imaging using a time-of-flight secondary ion mass spectrometry (TOF-SIMS) method. This technique was used to map the variations in lipid composition of the rat brain and, particularly, of the white matter. Triplicate analysis showed a significant increase of sulfatides (25%-50%) in the white matter on Day 10 of life in iNO-exposed animals from Day 0-7 of life. These robust, repeatable and semi-quantitative data demonstrate a potent effect of iNO at the molecular level.
authors.affiliation: Centre de Recherche de Gif, Institut de Chimie des Substances Naturelles, CNRS, Avenue de la Terrasse, Gif-sur-Yvette Cedex 91198, France. hananekadar@yahoo.fr.
authors.forename: Hanane
authors.initials: H
authors.lastname: Kadar
authors.affiliation: Institut National de la Santé Et de la Recherche Médicale (INSERM) U1141, Université Paris Diderot, PRES Sorbonne Paris-cité, Hôpital Robert Debré, 48 Boulevard Sérurier, Paris 75019, France. hoahp83@yahoo.com.
authors.forename: Hoa
authors.initials: H
authors.lastname: Pham
authors.affiliation: Centre de Recherche de Gif, Institut de Chimie des Substances Naturelles, CNRS, Avenue de la Terrasse, Gif-sur-Yvette Cedex 91198, France. David.Touboul@cnrs.fr.
authors.forename: David
authors.initials: D
authors.lastname: Touboul
authors.affiliation: Centre de Recherche de Gif, Institut de Chimie des Substances Naturelles, CNRS, Avenue de la Terrasse, Gif-sur-Yvette Cedex 91198, France. Alain.Brunelle@cnrs.fr.
authors.forename: Alain
authors.initials: A
authors.lastname: Brunelle
authors.affiliation: Institut National de la Santé Et de la Recherche Médicale (INSERM) U1141, Université Paris Diderot, PRES Sorbonne Paris-cité, Hôpital Robert Debré, 48 Boulevard Sérurier, Paris 75019, France. olivier.baud@rdb.aphp.fr.
authors.forename: Olivier
authors.initials: O
authors.lastname: Baud
mesh_terms: D000280:Administration, Inhalation; D000818:Animals; D001930:Brain Injuries / Q000188:drug therapy / Q000517:prevention & control*; D001931:Brain Mapping; D013058:Mass Spectrometry; D018696:Neuroprotective Agents / Q000494:pharmacology*; D009569:Nitric Oxide / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013433:Sulfoglycosphingolipids / Q000032:analysis*; D066127:White Matter / Q000187:drug effects / Q000196:embryology* / Q000473:pathology
chemical_list: D018696:Neuroprotective Agents; D013433:Sulfoglycosphingolipids; D009569:Nitric Oxide