title: Low expression of long noncoding XLOC_010588 indicates a poor prognosis and promotes proliferation through upregulation of c-Myc in cervical cancer.
abstract: OBJECTIVE
  The identification and investigation of cancer-associated long non-coding RNAs are important for understanding the molecular biology of cancer. The aim of the present study was to examine the expression pattern of lncRNA XLOC_010588 in cervical cancer and to evaluate its biological role and clinical significance in tumor progression.
  
  
  METHODS
  We examined the expression of XLOC_010588 in 218 cervical cancer tissues and matched 218 adjacent normal tissues using real-time qRT-PCR. Over-expression and RNA interference approaches were used to investigate the biological functions of XLOC_010588. The effect of XLOC_010588 on proliferation was evaluated by MTT and BrdU assays. Western blot assays were used to investigate the molecular mechanism by which XLOC_010588 increases cervical cancer cell proliferation.
  
  
  RESULTS
  The results showed that XLOC_010588 expression in cervical cancer was significantly downregulated. Decreased XLOC_010588 expression was correlated with FIGO stage, tumor size and SCC-Ag. Moreover, cervical cancer patients with XLOC_010588 lower expression have shown poorer prognosis. Multivariate Cox regression analyses showed that XLOC_010588 expression served as an independent predictor for overall survival. Ectopic expression of XLOC_010588 inhibited the proliferation of HeLa and SiHa cells. By contrast, knockdown of XLOC_010588 promoted the growth of HCC94 cells. Western blot assays confirmed that XLOC_010588 physically associates with c-Myc, consequently decreasing the expression of c-Myc. The expression of XLOC_010588 and c-Myc is strongly correlated in cervical cancer tissues.
  
  
  CONCLUSIONS
  These results suggested that XLOC_010588 plays a pivotal role in cervical cancer cell proliferation via decreasing c-Myc expression and implicated the potential application of XLOC_010588 in cervical cancer therapy.
authors.affiliation: Department of Ultrasound, The Second Affiliated Hospital of Nanchang University, Nanchang, China.
authors.forename: Ling-Min
authors.initials: LM
authors.lastname: Liao
authors.affiliation: Department of Gynecology and Obstetrics, Central Hospital Attached to Shenyang Medical College, Shenyang, China.
authors.forename: Xiao-Ying
authors.initials: XY
authors.lastname: Sun
authors.affiliation: Department of Oncology, The Second Affiliated Hospital of Nanchang University, Nanchang, China.
authors.forename: An-Wen
authors.initials: AW
authors.lastname: Liu
authors.affiliation: Department of Oncology, The Second Affiliated Hospital of Nanchang University, Nanchang, China.
authors.forename: Jian-Bing
authors.initials: JB
authors.lastname: Wu
authors.affiliation: Department of Medical Imaging, Women And Children Health Institute Futian, Shenzhen, China.
authors.forename: Xiao-Ling
authors.initials: XL
authors.lastname: Cheng
authors.affiliation: Department of Gynecology, Cancer Center, Sun Yat-sen University, Guangzhou, China.
authors.forename: Jia-Xin
authors.initials: JX
authors.lastname: Lin
authors.affiliation: Department of Gynecology, Cancer Center, Sun Yat-sen University, Guangzhou, China.
authors.forename: Min
authors.initials: M
authors.lastname: Zheng
authors.affiliation: Department of Oncology, The Second Affiliated Hospital of Nanchang University, Nanchang, China. Electronic address: huanglong914@gmail.com.
authors.forename: Long
authors.initials: L
authors.lastname: Huang
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D000328:Adult; D014408:Biomarkers, Tumor / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D016022:Case-Control Studies; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006367:HeLa Cells; D006801:Humans; D011379:Prognosis; D016271:Proto-Oncogene Proteins c-myc / Q000378:metabolism*; D062085:RNA, Long Noncoding / Q000235:genetics* / Q000502:physiology; D015854:Up-Regulation; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; C489427:MYC protein, human; D016271:Proto-Oncogene Proteins c-myc; D062085:RNA, Long Noncoding; C000589576:TUSC8 long non-coding RNA, human
keywords: Cervical cancer; Long noncoding RNA; XLOC_010588; c-Myc