title: An evaluation of antigen capture assays for detecting active filarial antigens.
abstract: Lymphatic filariasis is a parasitic disease of tropical countries. This is a disfiguring and painful disease contracted in childhood, but the symptoms become apparent only in later years. Diagnosis of filarial infection is very crucial for the management of the disease. The main objective of this study was to develop a filarial antigen-based immunological assay for the diagnosis and surveillance of the disease. Monoclonal and polyclonal antibodies were raised to the recombinant protein Brugia malayi vespid allergen homologue (VAH). Capture enzyme-linked immunosorbent assay (ELISA) was standardized utilizing various combinations of antibodies and evaluated with serum samples of endemic normal (EN, n= 110), microfilaraemic (MF, n= 65), chronic pathology (CP, n= 45) and non-endemic normal (NEN, n= 10) individuals. Of the 230 samples tested, VAH capture assay detected circulating antigen in 97.91% of bancroftian and 100% of brugian microfilaraemic individuals, and 5% of endemic normal individuals, comparable to the earlier reported SXP-1 antigen detection assay. However, the combination of VAH and SXP-1 (VS) capture ELISA was found to be more robust, detecting 100% of microfilaraemic individuals and with higher binding values. Thus an antigen capture immunoassay has been developed, which can differentiate active infection from chronic infection by detecting circulating filarial antigens in clinical groups of endemic areas.
authors.affiliation: Center for Biotechnology, Anna University,Chennai,Tamil Nadu,India.
authors.forename: R
authors.initials: R
authors.lastname: Ravishankaran
authors.affiliation: Department of Biochemistry,Indian Institute of Science,Bangalore,Karnataka,India.
authors.forename: N S
authors.initials: NS
authors.lastname: Radhika
authors.affiliation: Center for Biotechnology, Anna University,Chennai,Tamil Nadu,India.
authors.forename: L
authors.initials: L
authors.lastname: Ansel Vishal
authors.affiliation: Center for Biotechnology, Anna University,Chennai,Tamil Nadu,India.
authors.forename: S
authors.initials: S
authors.lastname: Meenakshisundaram
authors.affiliation: Department of Biochemistry,Indian Institute of Science,Bangalore,Karnataka,India.
authors.forename: A A
authors.initials: AA
authors.lastname: Karande
authors.affiliation: Center for Biotechnology, Anna University,Chennai,Tamil Nadu,India.
authors.forename: P
authors.initials: P
authors.lastname: Kaliraj
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis*; D017178:Brugia malayi / Q000302:isolation & purification*; D004605:Elephantiasis, Filarial / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D000947:Antigens, Helminth