title: Identification and characterization of the expression profile of microRNAs in Anopheles anthropophagus.
abstract: BACKGROUND
  Anopheles anthropophagus, one of the most important mosquito-borne disease vectors in Asia, mainly takes blood meals from humans and transmits both malaria and filariae. MicroRNAs (miRNAs) are small non-coding RNAs, and play a critical role in many cellular processes, including development, differentiation, apoptosis and innate immunity.
  
  
  METHODS
  We investigated the global miRNA expression profile of male and female adults of A. anthropophagus using illumina Hiseq2000 sequencing combined with Northern blot.
  
  
  RESULTS
  By using the miRNAs of the closely-related species Anopheles gambiae and Aedes aegypti as reference, we obtained 102 miRNAs candidates out of 12.43 million raw sequencing reads for male and 16.51 million reads for female, with 81 of them found as known miRNAs in An. gambiae and/or Ae. aegypti, and the remaining 21 miRNAs were considered as novel. By analyzing the revised read count of miRNAs in male and female, 29 known miRNAs show sexual difference expression: >2-fold in the read count of the same miRNAs in male and female. Especially for miR-989, which is highly expressed in the female mosquitoes, but shows almost no detected expression in male mosquitoes, indicating that miR-989 may be involved in the physiological activity of female mosquito adults. The expression of four miRNAs in different growth stages of mosquito were further identified by Northern blot. Several miRNAs show the stage-specific expression, of which miR-2943 only expressed in the egg stage, suggesting that miR-2943 may be associated with the development of mosquito eggs.
  
  
  CONCLUSIONS
  The present study represents the first global characterization of An. anthropophagus miRNAs in sexual differences and stage-specific functions. A better understanding of the functions of these miRNAs will offer new insights in mosquito biology and has implications for the effective control of mosquito-borne infectious diseases.
authors.forename: Wenquan
authors.initials: W
authors.lastname: Liu
authors.forename: Huicong
authors.initials: H
authors.lastname: Huang
authors.forename: Cuicui
authors.initials: C
authors.lastname: Xing
authors.forename: Chunxiang
authors.initials: C
authors.lastname: Li
authors.forename: Feng
authors.initials: F
authors.lastname: Tan
authors.affiliation: Department of Parasitology, Wenzhou Medical University, Wenzhou, Zhejiang Province 325035, PR China. lsh@wmu.edu.cn.
authors.forename: Shaohui
authors.initials: S
authors.lastname: Liang
mesh_terms: D000818:Animals; D000852:Anopheles / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D059467:Transcriptome
chemical_list: D035683:MicroRNAs