title: Analysis of mouse RPE sheet morphology gives discriminatory categories.
abstract: We are interested in developing quantitative tools to study retinal pigmented epithelium (RPE) morphology. We want to detect changes in the RPE by strain, disease, genotype, and age. Ultimately these tools should be useful in predicting retinal disease progression. The morphometric data will also help us to understand RPE sheet formation and barrier functions. A clear disruption of the regular cell size and shape appeared in mouse mutants. Aspect ratio and cell area together gave rise to principal components that predicted age and genotype accurately and well before visually obvious damage could be seen.
authors.affiliation: Department of Mathematics and Statistics, Georgia State University, Atlanta, GA, USA, yjiang12@gsu.edu.
authors.forename: Yi
authors.initials: Y
authors.lastname: Jiang
authors.forename: X
authors.initials: X
authors.lastname: Qi
authors.forename: Micah A
authors.initials: MA
authors.lastname: Chrenek
authors.forename: Christopher
authors.initials: C
authors.lastname: Gardner
authors.forename: Nupur
authors.initials: N
authors.lastname: Dalal
authors.forename: Jeffrey H
authors.initials: JH
authors.lastname: Boatright
authors.forename: Hans E
authors.initials: HE
authors.lastname: Grossniklaus
authors.forename: John M
authors.initials: JM
authors.lastname: Nickerson
mesh_terms: D000818:Animals; D054707:Cyclic Nucleotide Phosphodiesterases, Type 6 / Q000235:genetics; D018450:Disease Progression; D005838:Genotype; D008268:Macular Degeneration / Q000235:genetics / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D012160:Retina / Q000473:pathology*; D055213:Retinal Pigment Epithelium / Q000473:pathology*; D019745:cis-trans-Isomerases / Q000235:genetics; D000099178:Retinoid Isomerohydrolase
chemical_list: D054707:Cyclic Nucleotide Phosphodiesterases, Type 6; D019745:cis-trans-Isomerases; D000099178:Retinoid Isomerohydrolase; C517438:Pde6b protein, mouse