title: Reaction mechanism of the bicopper enzyme peptidylglycine α-hydroxylating monooxygenase.
abstract: Peptidylglycine α-hydroxylating monooxygenase is a noninteracting bicopper enzyme that stereospecifically hydroxylates the terminal glycine of small peptides for its later amidation. Neuroendocrine messengers, such as oxytocin, rely on the biological activity of this enzyme. Each catalytic turnover requires one oxygen molecule, two protons from the solvent, and two electrons. Despite this enzyme having been widely studied, a consensus on the reaction mechanism has not yet been found. Experiments and theoretical studies favor a pro-S abstraction of a hydrogen atom followed by the rebinding of an OH group. However, several hydrogen-abstracting species have been postulated; because two protons are consumed during the reaction, several protonation states are available. An electron transfer between the copper atoms could play a crucial role for the catalysis as well. This leads to six possible abstracting species. In this study, we compare them on equal footing. We perform quantum mechanics/molecular mechanics calculations, considering the glycine hydrogen abstraction. Our results suggest that the most likely mechanism is a protonation of the abstracting species before the hydrogen abstraction and another protonation as well as a reduction before OH rebinding.
authors.affiliation: From the Computational Biochemistry Group, Institute of Theoretical Chemistry, University of Stuttgart, Pfaffenwaldring 55, 70569 Stuttgart, Germany.
authors.forename: Enrique
authors.initials: E
authors.lastname: Abad
authors.affiliation: From the Computational Biochemistry Group, Institute of Theoretical Chemistry, University of Stuttgart, Pfaffenwaldring 55, 70569 Stuttgart, Germany.
authors.forename: Judith B
authors.initials: JB
authors.lastname: Rommel
authors.affiliation: From the Computational Biochemistry Group, Institute of Theoretical Chemistry, University of Stuttgart, Pfaffenwaldring 55, 70569 Stuttgart, Germany kaestner@theochem.uni-stuttgart.de.
authors.forename: Johannes
authors.initials: J
authors.lastname: Kästner
mesh_terms: D020134:Catalytic Domain; D003300:Copper / Q000378:metabolism*; D004579:Electron Transport; D006860:Hydrogen Bonding; D006900:Hydroxylation; D006899:Mixed Function Oxygenases / Q000737:chemistry / Q000378:metabolism*; D056004:Molecular Dynamics Simulation; D009097:Multienzyme Complexes / Q000737:chemistry / Q000378:metabolism*
chemical_list: D009097:Multienzyme Complexes; D003300:Copper; D006899:Mixed Function Oxygenases; C035189:peptidylglycine monooxygenase
keywords: Computer Modeling; Copper; Enzyme Catalysis; Kinetic Isotope Effects; Metalloproteins; PHM; Post-translational Modification; QM/MM Simulation; Quantum Tunneling; Reaction Mechanism