title: Isolation and characterization of novel thermophilic lipase-secreting bacteria.
abstract: The purpose of the present study was to screen and identify the lipase-producing microorganisms from various regions of Iran. Samples collected from hot spring, Persian Gulf, desert area and oil-contaminated soil, were analyzed for thermophilic extracellular-lipase producing organisms. Six strains with high activity on rhodamine B plates were selected for chemical identification and further study. Among these isolated bacteria, four strains show higher activity in pH-Stat method at 55 °C. These strains were identified by PCR amplification of 16s rRNA genes using universal primers. Fermentation increased the activity up to 50%. The growth medium, designed for lipase production, increased the activity up to 4.55 folds. The crude supernatant of ZR-5 after fermentation and separation the cells, was lyophilized and the activity was measured. Total activity of this strain was 12 kU/g that shows its potential for industrial uses. Further study is required for purification of enzyme and calculation its specific activity. Immobilization is another approach should be considered.
authors.affiliation: Department of Pharmaceutical Biotechnology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Mohammed
authors.initials: M
authors.lastname: Rabbani
authors.affiliation: Department of Pharmaceutical Biotechnology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Bagherinejad
authors.affiliation: Department of Pharmaceutical Biotechnology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Hamid MirMohammad
authors.initials: HM
authors.lastname: Sadeghi
authors.affiliation: Department of Biochemistry, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Ziaedin Samsam
authors.initials: ZS
authors.lastname: Shariat
authors.affiliation: Department of Biology, School of Basic Sciences, University of Isfahan, Isfahan, Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Etemadifar
authors.affiliation: Department of Pharmaceutical Biotechnology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Moazen
authors.affiliation: Department of Biology, School of Basic Sciences, University of Isfahan, Isfahan, Iran.
authors.forename: Manizheh
authors.initials: M
authors.lastname: Rahbari
authors.affiliation: Department of Biology, School of Basic Sciences, University of Isfahan, Isfahan, Iran.
authors.forename: Ladan
authors.initials: L
authors.lastname: Mafakher
authors.affiliation: Department of Biology, School of Basic Sciences, University of Isfahan, Isfahan, Iran.
authors.forename: Saeideh
authors.initials: S
authors.lastname: Zaghian
mesh_terms: D001419:Bacteria / Q000145:classification / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification*; D015373:Bacterial Typing Techniques; D003470:Culture Media / Q000737:chemistry; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004783:Environmental Microbiology; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D007492:Iran; D008049:Lipase / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013696:Temperature
chemical_list: D003470:Culture Media; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D008049:Lipase
keywords: extracellular lipase; fermentation; pH-stat method; thermophilic lipase