title: Comparison of 16S rRNA sequencing with biochemical testing for species-level identification of clinical isolates of Neisseria spp.
abstract: We aimed to compare accuracy of genus and species level identification of Neisseria spp. using biochemical testing and 16S rRNA sequence analysis. These methods were evaluated using 85 Neisseria spp. clinical isolates initially identified to the genus level by conventional biochemical tests and API NH system (Bio-Mérieux(®)). In 34 % (29/85), more than one possibility was given by 16S rRNA sequence analysis. In 6 % (5/85), one of the possibilities offered by 16S rRNA gene sequencing, agreed with the result given by biochemical testing. In 4 % (3/85), the same species was given by both methods. 16S rRNA gene sequencing results did not correlate well with biochemical tests.
authors.affiliation: Service des Laboratoires (UR12ES02), Centre National de Greffe de Moelle Osseuse, Rue Djebel-Lakdhar, Bab Saadoun, 1006, Tunis, Tunisia, arij.mechergui@gmail.com.
authors.forename: Arij
authors.initials: A
authors.lastname: Mechergui
authors.forename: Wafa
authors.initials: W
authors.lastname: Achour
authors.forename: Assia
authors.initials: A
authors.lastname: Ben Hassen
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods*; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology*; D009343:Neisseria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D012329:RNA, Bacterial / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D017423:Sequence Analysis, RNA / Q000379:methods
chemical_list: D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S