title: Health effects and arsenic species in urine of copper smelter workers.
abstract: The aim of this study was to compare indices of exposure in workers employed at different work posts in a copper smelter plant using neurophysiological tests and to evaluate the relationship between urinary arsenic species with the aid of sensitive respiratory and renal biomarkers. We have attempted to elucidate the impact of different arsenic speciation forms on the observed health effects. We focused on the workers (n = 45) exposed to atmospheres containing specific diverse mixtures of metals (such as those occurring in Departments of Furnaces, Lead and Electrolysis) compared to controls (n = 16). Subjective symptoms from the central (CNS) and the peripheral (PNS) nervous system were recorded and visual evoked potential (VEP), electroneurography (ENeG) and electroencephalography (EEG) curves were analysed. Levels of airborne lead (PbA), zinc (ZnA) and copper (CuA) and Pb levels in blood (PbB) and the relationships between airborne As concentrations (AsA) and the urinary levels of the inorganic (iAs); As(+3), As(+5) and the organic; methylarsonate (MMA(V)), dimethylarsinate (DMA(V)) and arsenobetaine (AsB) arsenic species were determined by Inductively Coupled Plasma Mass Spectrometry (ICP-MS). Effects of exposure were expressed in terms of biomarker levels: Clara cell protein (CC16) in serum as early pulmonary biomarker and β2-microglobulin (β2M) in urine and serum, retinol binding protein (RBP) as renal markers, measured by sensitive latex-immunoassay (LIA). Abnormal results of neurophysiological tests, VEP, EEG and ENeG showed dominant subclinical effects in CNS and PNS of workers from Departments of Lead and Furnace. In group of smelters from Departments of Furnace exposed to arsenic above current TLV, excreted arsenic species As(+3) and As(+5) seemed to reduce the level of Clara cell protein (CC16), thereby reducing anti-inflammatory potential of the lungs and increasing the levels of renal biomarker (β2M) and copper in urine (CuU). The study confirmed deleterious arsenic effects to the kidney by increased levels of low-molecular weight protein in urine and the extent of the renal copper accumulation/excretion. The results of our work also support the usefulness of application of the sensitive neurophysiologic tests, such as VEP, EEG and ENeG, for the detection of early subclinical effects of the exposure of the nervous system in copper smelters.
authors.affiliation: a Department of Toxicology and Carcinogenesis , Nofer Institute of Occupational Medicine , Lodz , Poland.
authors.forename: Tadeusz
authors.initials: T
authors.lastname: Halatek
authors.forename: Halina
authors.initials: H
authors.lastname: Sinczuk-Walczak
authors.forename: Beata
authors.initials: B
authors.lastname: Janasik
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Trzcinka-Ochocka
authors.forename: Renata
authors.initials: R
authors.lastname: Winnicka
authors.forename: Wojciech
authors.initials: W
authors.lastname: Wasowicz
mesh_terms: D000328:Adult; D016902:Air Pollution, Indoor / Q000032:analysis*; D000704:Analysis of Variance; D001151:Arsenic / Q000652:urine*; D001152:Arsenicals / Q000652:urine; D015415:Biomarkers / Q000097:blood; D002101:Cacodylic Acid / Q000652:urine; D003300:Copper; D004569:Electroencephalography; D005074:Evoked Potentials, Visual / Q000502:physiology; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D008669:Metallurgy; D019216:Metals, Heavy / Q000032:analysis; D008875:Middle Aged; D009420:Nervous System / Q000187:drug effects* / Q000503:physiopathology; D064926:Neurophysiological Monitoring; D016273:Occupational Exposure / Q000009:adverse effects*; D013147:Spirometry
chemical_list: D001152:Arsenicals; D015415:Biomarkers; D019216:Metals, Heavy; D003300:Copper; D002101:Cacodylic Acid; C020300:monomethylarsonic acid; D001151:Arsenic; C038992:arsenobetaine