title: Comparative biomechanical and microstructural analysis of native versus peracetic acid-ethanol treated cancellous bone graft.
abstract: Bone transplantation is frequently used for the treatment of large osseous defects. The availability of autologous bone grafts as the current biological gold standard is limited and there is a risk of donor site morbidity. Allogenic bone grafts are an appealing alternative, but disinfection should be considered to reduce transmission of infection disorders. Peracetic acid-ethanol (PE) treatment has been proven reliable and effective for disinfection of human bone allografts. The purpose of this study was to evaluate the effects of PE treatment on the biomechanical properties and microstructure of cancellous bone grafts (CBG). Forty-eight human CBG cylinders were either treated by PE or frozen at -20 °C and subjected to compression testing and histological and scanning electron microscopy (SEM) analysis. The levels of compressive strength, stiffness (Young's modulus), and fracture energy were significantly decreased upon PE treatment by 54%, 59%, and 36%, respectively. Furthermore, PE-treated CBG demonstrated a 42% increase in ultimate strain. SEM revealed a modified microstructure of CBG with an exposed collagen fiber network after PE treatment. We conclude that the observed reduced compressive strength and reduced stiffness may be beneficial during tissue remodeling thereby explaining the excellent clinical performance of PE-treated CBG.
authors.affiliation: University Centre for Orthopaedics and Traumatology, University Hospital Carl Gustav Carus, Technische Universität Dresden, 01307 Dresden, Germany ; Centre for Translational Bone, Joint and Soft Tissue Research, University Hospital and Medical Faculty Carl Gustav Carus, Technische Universität Dresden, 01307 Dresden, Germany.
authors.forename: Juliane
authors.initials: J
authors.lastname: Rauh
authors.affiliation: Centre for Translational Bone, Joint and Soft Tissue Research, University Hospital and Medical Faculty Carl Gustav Carus, Technische Universität Dresden, 01307 Dresden, Germany.
authors.forename: Florian
authors.initials: F
authors.lastname: Despang
authors.affiliation: Orthopaedic Research Laboratory, Aarhus University Hospital, 8000 Aarhus, Denmark.
authors.forename: Jorgen
authors.initials: J
authors.lastname: Baas
authors.affiliation: University Centre for Orthopaedics and Traumatology, University Hospital Carl Gustav Carus, Technische Universität Dresden, 01307 Dresden, Germany ; Centre for Translational Bone, Joint and Soft Tissue Research, University Hospital and Medical Faculty Carl Gustav Carus, Technische Universität Dresden, 01307 Dresden, Germany.
authors.forename: Cornelia
authors.initials: C
authors.lastname: Liebers
authors.affiliation: Institute of Transfusion Medicine, Tissue Bank, Charité-Universitätsmedizin Berlin, 10117 Berlin, Germany.
authors.forename: Axel
authors.initials: A
authors.lastname: Pruss
authors.affiliation: Centre for Translational Bone, Joint and Soft Tissue Research, University Hospital and Medical Faculty Carl Gustav Carus, Technische Universität Dresden, 01307 Dresden, Germany.
authors.forename: Michael
authors.identifier: 0000-0001-9075-5121
authors.initials: M
authors.lastname: Gelinsky
authors.affiliation: University Centre for Orthopaedics and Traumatology, University Hospital Carl Gustav Carus, Technische Universität Dresden, 01307 Dresden, Germany ; Centre for Translational Bone, Joint and Soft Tissue Research, University Hospital and Medical Faculty Carl Gustav Carus, Technische Universität Dresden, 01307 Dresden, Germany.
authors.forename: Klaus-Peter
authors.initials: KP
authors.lastname: Günther
authors.affiliation: University Centre for Orthopaedics and Traumatology, University Hospital Carl Gustav Carus, Technische Universität Dresden, 01307 Dresden, Germany ; Centre for Translational Bone, Joint and Soft Tissue Research, University Hospital and Medical Faculty Carl Gustav Carus, Technische Universität Dresden, 01307 Dresden, Germany.
authors.forename: Maik
authors.initials: M
authors.lastname: Stiehler
mesh_terms: D000328:Adult; D001696:Biomechanical Phenomena / Q000187:drug effects; D015519:Bone Density / Q000187:drug effects; D016025:Bone Transplantation; D001842:Bone and Bones / Q000033:anatomy & histology* / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D019245:Compressive Strength / Q000187:drug effects; D000431:Ethanol / Q000494:pharmacology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010463:Peracetic Acid / Q000494:pharmacology*; D013314:Stress, Mechanical; D055815:Young Adult
chemical_list: D000431:Ethanol; D010463:Peracetic Acid