title: Role of CDK5/cyclin complexes in ischemia-induced death and survival of renal tubular cells.
abstract: Ischemia reperfusion processes induce damage in renal tubules and compromise the viability of kidney transplants. Understanding the molecular events responsible for tubule damage and recovery would help to develop new strategies for organ preservation. CDK5 has been traditionally considered a neuronal kinase with dual roles in cell death and survival. Here, we demonstrate that CDK5 and their regulators p35/p25 and cyclin I are also expressed in renal tubular cells. We show that treatment with CDK inhibitors promotes the formation of pro-survival CDK5/cyclin I complexes and enhances cell survival upon an ischemia reperfusion pro-apoptotic insult. These findings support the benefit of treating with CDK inhibitors for renal preservation, assisting renal tubule protection.
authors.affiliation: Laboratory of Peptide and Protein Chemistry; Centro de Investigación Príncipe Felipe; Valencia, Spain; Instituto de Biomedicina de Valencia; IBV-CSIC; Valencia, Spain.
authors.forename: Tatiana
authors.initials: T
authors.lastname: Guevara
authors.affiliation: Laboratory of Peptide and Protein Chemistry; Centro de Investigación Príncipe Felipe; Valencia, Spain.
authors.forename: Mónica
authors.initials: M
authors.lastname: Sancho
authors.affiliation: Laboratory of Peptide and Protein Chemistry; Centro de Investigación Príncipe Felipe; Valencia, Spain; Instituto de Biomedicina de Valencia; IBV-CSIC; Valencia, Spain.
authors.forename: Enrique
authors.initials: E
authors.lastname: Pérez-Payá
authors.affiliation: Laboratory of Peptide and Protein Chemistry; Centro de Investigación Príncipe Felipe; Valencia, Spain.
authors.forename: Mar
authors.initials: M
authors.lastname: Orzáez
mesh_terms: D000818:Animals; D016923:Cell Death; D015687:Cell Hypoxia; D002460:Cell Line; D002470:Cell Survival; D019925:Cyclin A / Q000378:metabolism*; D051360:Cyclin-Dependent Kinase 5 / Q000378:metabolism*; D007684:Kidney Tubules / Q000166:cytology* / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics; D013552:Swine
chemical_list: D019925:Cyclin A; D009419:Nerve Tissue Proteins; C089495:neuronal Cdk5 activator (p25-p35); D051360:Cyclin-Dependent Kinase 5
keywords: CDK5; apoptosis; ischemia reperfusion; kidney injury; renal preservation