title: Using a yeast two-hybrid system to identify FTCD as a new regulator for HIF-1α in HepG2 cells.
abstract: HIF-1α is implicated in hepatocellular carcinoma (HCC) pathologies. Here, we screened a human liver cDNA library for HIF-1α-interacting partners using a yeast two-hybrid system. We identified 53 genes, including formiminotransferase cyclodeaminase (FTCD), which was confirmed by co-immunoprecipitation. Moreover, our data indicated that HIF-1α mediated the effects of hypoxia on FTCD induction via binding to the hypoxia-responsive elements of the FTCD promoter. Knockdown of FTCD reduced the effects of HIF-1α in hypoxia and enhanced chemosensitivity in HepG2 cells. Our findings suggested crosstalk between FTCD and HIF signaling and promoted HCC progression, thus implicating FTCD as a therapeutic target for HCC.
authors.affiliation: Department of Nuclear Medicine, Ren ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China; School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, China.
authors.forename: Zhenhai
authors.initials: Z
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510006, China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Ge
authors.affiliation: Department of Nuclear Medicine, Ren ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Xie
authors.affiliation: Department of Nuclear Medicine, Ren ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China.
authors.forename: Teng
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Institute of Health Sciences, Shanghai Jiao Tong University School of Medicine (SJTUSM) & Shanghai Institutes for Biological Sciences (SIBS), Chinese Academy of Sciences (CAS), Shanghai 200025, China.
authors.forename: Liangqian
authors.initials: L
authors.lastname: Huang
authors.affiliation: Department of Nuclear Medicine, Ren ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Nuclear Medicine, Ren ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Nuclear Medicine, Ren ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China; School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, China; Institute of Health Sciences, Shanghai Jiao Tong University School of Medicine (SJTUSM) & Shanghai Institutes for Biological Sciences (SIBS), Chinese Academy of Sciences (CAS), Shanghai 200025, China. Electronic address: huang2802@163.com.
authors.forename: Gang
authors.initials: G
authors.lastname: Huang
mesh_terms: D000642:Ammonia-Lyases / Q000096:biosynthesis / Q000378:metabolism*; D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D015687:Cell Hypoxia / Q000502:physiology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D051042:Glutamate Formimidoyltransferase / Q000096:biosynthesis / Q000378:metabolism*; D056945:Hep G2 Cells; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics*; D008113:Liver Neoplasms / Q000473:pathology*; D064251:Multifunctional Enzymes; D009361:Neoplasm Invasiveness / Q000473:pathology; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D034622:RNA Interference; D034741:RNA, Small Interfering; D020218:Response Elements / Q000235:genetics; D015398:Signal Transduction; D020798:Two-Hybrid System Techniques
chemical_list: C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D064251:Multifunctional Enzymes; D034741:RNA, Small Interfering; C494848:FTCD protein, human; D051042:Glutamate Formimidoyltransferase; D000642:Ammonia-Lyases
keywords: Chemosensitivity; FTCD; HIF-1α; Hypoxia; Yeast two-hybrid