title: Coimmunoprecipitation of proteins from yeast.
abstract: This protocol outlines a procedure for testing whether two proteins interact. A target protein will be immunoprecipitated using an antibody that recognizes it (or a tagged version of the protein). The immunoprecipitated material will be separated by SDS-PAGE and analyzed by Western blotting to assess the presence of a candidate interacting protein(s).
authors.affiliation: Department of Cell Biology, Howard Hughes Medical Institute, Harvard Medical School, Boston, MA, USA.
authors.forename: Erica
authors.initials: E
authors.lastname: Gerace
authors.affiliation: Department of Cell Biology, Howard Hughes Medical Institute, Harvard Medical School, Boston, MA, USA. Electronic address: danesh@hms.harvard.edu.
authors.forename: Danesh
authors.initials: D
authors.lastname: Moazed
mesh_terms: D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000737:chemistry* / Q000378:metabolism; D047468:Immunoprecipitation / Q000295:instrumentation / Q000379:methods*; D025941:Protein Interaction Mapping; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins / Q000737:chemistry / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins
keywords: Coimmunoprecipitation; Immunoprecipitations; Normalization of cell lysates; Protein–protein interactions; Schizosaccharomyces pombe; Sodium dodecyl sulfate polyacrylamide gel electrophoresis; Western blotting; Whole cell lysates