title: Involvement of amygdaloid neuropeptide Y in the anxiolytic effects of acupuncture during ethanol withdrawal in rats.
abstract: The role of neuropeptide Y (NPY) in the central nucleus of amygdala (CeA) in the preventive effects of acupuncture against ethanol withdrawal-induced anxiety was investigated. Rats were treated with 3g/kg/day of ethanol for 28 days, followed by 3 days of withdrawal. Bilateral acupuncture treatment at HT7 (Shen-Men), PC6 (Nei-Guan) or a non-acupoint was respectively added to the rats during the withdrawal once a day for three days. Enzyme-linked immunosorbent assays and real-time polymerase chain reaction analyses showed there was a significant decrease in NPY protein and mRNA expression in the CeA during ethanol withdrawal, which was reversed by acupuncture at HT7 but neither at PC6 nor at a non-acupoint. Acupuncture at HT7 also greatly inhibited the decrease in cAMP response element-binding protein (CREB) phosphorylation in the CeA. In elevated plus maze tests, a selective NPY Y1 receptor antagonist BIBP 3226 into the CeA before the acupuncture abolished almost completely the anxiolytic effect of acupuncture at HT7. These results suggest that acupuncture at HT7 rescues the depletion of amygdaloid NPY and reverses the decrease in CREB phosphorylation to produce anxiolytic effects during ethanol withdrawal.
authors.affiliation: Department of Pharmacology, Mudanjiang Medical University, Mudanjiang, China.
authors.forename: Zhenglin
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: College of Oriental Medicine, Daegu Haany University, Daegu, Republic of Korea.
authors.forename: Sang Chan
authors.initials: SC
authors.lastname: Kim
authors.affiliation: Department of Pharmacology, Mudanjiang Medical University, Mudanjiang, China.
authors.forename: Yiyan
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Pharmacology, Mudanjiang Medical University, Mudanjiang, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Preventive Medicine, Hygiene, Yanbian University, Yanji, China.
authors.forename: Yanji
authors.initials: Y
authors.lastname: Xu
authors.affiliation: College of Oriental Medicine, Daegu Haany University, Daegu, Republic of Korea.
authors.forename: Il Je
authors.initials: IJ
authors.lastname: Cho
authors.affiliation: College of Oriental Medicine, Daegu Haany University, Daegu, Republic of Korea.
authors.forename: Chae Ha
authors.initials: CH
authors.lastname: Yang
authors.affiliation: College of Oriental Medicine, Daegu Haany University, Daegu, Republic of Korea. Electronic address: dlqhdgy@dhu.ac.kr.
authors.forename: Bong Hyo
authors.initials: BH
authors.lastname: Lee
authors.affiliation: Department of Pharmacology, Mudanjiang Medical University, Mudanjiang, China; College of Oriental Medicine, Daegu Haany University, Daegu, Republic of Korea. Electronic address: zhao_rongjie@yahoo.com.
authors.forename: Rongjie
authors.initials: R
authors.lastname: Zhao
mesh_terms: D015670:Acupuncture Therapy; D000679:Amygdala / Q000378:metabolism*; D000818:Animals; D001007:Anxiety / Q000523:psychology / Q000628:therapy*; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D001552:Benzazepines / Q000494:pharmacology; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism; D000431:Ethanol / Q000009:adverse effects*; D008297:Male; D018782:Maze Learning; D009478:Neuropeptide Y / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D012333:RNA, Messenger / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D017476:Receptors, Neuropeptide Y / Q000037:antagonists & inhibitors; D013375:Substance Withdrawal Syndrome / Q000523:psychology / Q000628:therapy*
chemical_list: C092926:BIBP 3226; D001552:Benzazepines; D017362:Cyclic AMP Response Element-Binding Protein; D009478:Neuropeptide Y; D012333:RNA, Messenger; D017476:Receptors, Neuropeptide Y; C097212:neuropeptide Y-Y1 receptor; D000431:Ethanol; D001120:Arginine; C403256:BIIE 0246
keywords: Acupuncture; Amygdala; Ethanol withdrawal; Neuropeptide Y; cAMP response element-binding protein