title: Extracellular polysaccharide production by a strain of Pleurotus djamor isolated in the south of Brazil and antitumor activity on Sarcoma 180.
abstract: Polysaccharides with medicinal properties can be obtained from fruiting bodies, mycelium and culture broth of several fungus species. This work was carried out in batch culture using a stirred tank reactor with two different initial glucose concentrations (40-50 g/L) and pH values (3.0-4.0) to enhance extracellular polysaccharides production by Pleurotus djamor UNIVILLE 001 and evaluate antitumor effect of intraperitonial administration of Pleurotus djamor extract on sarcoma 180 animal model. According to factorial design, the low pH value (pH 3.0) led to a gain of 1.6 g/L on the extracellular polysaccharide concentration, while glucose concentration in the tested range had no significant effect on the concentration of polysaccharide. With 40 g/L initial glucose concentration and pH 3.0, it was observed that yield factor of extracellular polysaccharide on substrate (YP/S = 0.072) and maximum extracellular polysaccharide productivity (Q(Pmax) = 11.26 mg/L.h) were about 188% and 321% respectively higher than those obtained in the experiment performed at pH 4.0. Under these conditions, the highest values of the yield factor of biomass on substrate (YX/S = 0.24) and maximal biomass productivity (Q(Xmax) = 32.2 mg/L.h) were also reached. In tumor response study, mean tumor volume on the 21th day was 35.3 cm(3) in untreated group and 1.6 cm(3) in treated group (p = 0.05) with a tumor inhibition rate of 94%. These impressive results suggests an inhibitory effect of P.djamor extract on cancer cells.
authors.affiliation: Mestrado em Saúde e Meio Ambiente, Universidade da Região de Joinville, Univille, Joinville, SC, Brazil.
authors.forename: Gisele Martini
authors.initials: GM
authors.lastname: Borges
authors.affiliation: Mestrado em Saúde e Meio Ambiente, Universidade da Região de Joinville, Univille, Joinville, SC, Brazil.
authors.forename: Fabiana Figueredo Molin
authors.initials: FF
authors.lastname: De Barba
authors.affiliation: Departamento de Engenharia Ambiental, Universidade da Região de Joinville, Univille, Joinville, SC, Brazil.
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: Schiebelbein
authors.affiliation: Departamento de Farmácia, Universidade da Região de Joinville, Univille, Joinville, SC, Brazil.
authors.forename: Bruna Parmezzani
authors.initials: BP
authors.lastname: Pereira
authors.affiliation: Departamento de Engenharia Química, Universidade da Região de Joinville, Univille, Joinville, SC, Brazil.
authors.forename: Mariane Bonatti
authors.initials: MB
authors.lastname: Chaves
authors.affiliation: Departamento de Farmácia, Universidade da Região de Joinville, Univille, Joinville, SC, Brazil.
authors.forename: Marcia Luciane Lange
authors.initials: ML
authors.lastname: Silveira
authors.affiliation: Mestrado em Saúde e Meio Ambiente, Universidade da Região de Joinville, Univille, Joinville, SC, Brazil.
authors.forename: Mauro Souza Leite
authors.initials: MS
authors.lastname: Pinho
authors.affiliation: Mestrado em Saúde e Meio Ambiente, Universidade da Região de Joinville, Univille, Joinville, SC, Brazil. ; Departamento de Engenharia Ambiental, Universidade da Região de Joinville, Univille, Joinville, SC, Brazil. ; Departamento de Engenharia Química, Universidade da Região de Joinville, Univille, Joinville, SC, Brazil. ; Mestrado em Engenharia de Processos, Universidade da Região de Joinville, Univille, Joinville, SC, Brazil.
authors.forename: Sandra Aparecida
authors.initials: SA
authors.lastname: Furlan
authors.affiliation: Departamento de Engenharia Ambiental, Universidade da Região de Joinville, Univille, Joinville, SC, Brazil. ; Departamento de Engenharia Química, Universidade da Região de Joinville, Univille, Joinville, SC, Brazil. ; Mestrado em Engenharia de Processos, Universidade da Região de Joinville, Univille, Joinville, SC, Brazil.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Wisbeck
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000302:isolation & purification* / Q000378:metabolism / Q000627:therapeutic use*; D001938:Brazil; D003470:Culture Media / Q000737:chemistry; D004195:Disease Models, Animal; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007274:Injections, Intraperitoneal; D008297:Male; D051379:Mice; D020076:Pleurotus / Q000302:isolation & purification / Q000378:metabolism*; D011134:Polysaccharides / Q000302:isolation & purification* / Q000378:metabolism / Q000627:therapeutic use*; D012509:Sarcoma / Q000188:drug therapy*; D016896:Treatment Outcome
chemical_list: D000970:Antineoplastic Agents; D003470:Culture Media; D011134:Polysaccharides; D005947:Glucose
keywords: Pleurotus djamor; antitumor activity; extracellular polysaccharides production