title: In vitro evaluation of adherence of Candida albicans, Candida glabrata, and Streptococcus mutans to an acrylic resin modified by experimental coatings.
abstract: This study evaluated the effect of experimental coatings, containing zwitterion or hydrophilic monomers, on the adherence of Candida albicans, Candida glabrata, and Streptococcus mutans to an acrylic resin. Acrylic samples (smooth or rough surfaces) were left untreated (control) or coated with one of the following experimental coatings: 3-hydroxypropylmethacrylate (HP) or sulfobetaine methacrylate (S), at concentrations of 25, 30, or 35%. Half of the specimens were coated with saliva. The adhesion test was performed by incubating specimens in C. albicans, C. glabrata, and S. mutans suspensions at 37°C for 90 min. The number of adhered microorganisms was determined by metabolic activity (XTT) and by cell viability (CFU). All coated specimens exhibited lower absorbance and CFU values compared to control specimens. Saliva and roughness did not promote microorganism adherence. An XPS analysis confirmed the modification in the chemical composition of the coatings in the experimental samples. These experimental coatings significantly reduced the adherence of C. albicans, C. glabrata and S. mutans to acrylic resin.
authors.affiliation: a Department of Dental Materials and Prosthodontics, Araraquara Dental School , Universidade Estadual Paulista, UNESP , São Paulo , Brazil.
authors.forename: Fernanda Emiko
authors.initials: FE
authors.lastname: Izumida
authors.forename: Eduardo Buozi
authors.initials: EB
authors.lastname: Moffa
authors.forename: Carlos Eduardo
authors.initials: CE
authors.lastname: Vergani
authors.forename: Ana Lúcia
authors.initials: AL
authors.lastname: Machado
authors.forename: Janaína Habib
authors.initials: JH
authors.lastname: Jorge
authors.forename: Eunice Teresinha
authors.initials: ET
authors.lastname: Giampaolo
mesh_terms: D000180:Acrylic Resins; D001422:Bacterial Adhesion; D057219:Biofouling / Q000517:prevention & control*; D002176:Candida albicans / Q000502:physiology*; D041221:Candida glabrata / Q000502:physiology*; D002448:Cell Adhesion; D013295:Streptococcus mutans / Q000502:physiology*; D013499:Surface Properties
chemical_list: D000180:Acrylic Resins
keywords: Candida albicans; Candida glabrata; Streptococcus mutans; acrylic resin; saliva