title: A histone methylation network regulates transgenerational epigenetic memory in C. elegans.
abstract: How epigenetic information is transmitted from generation to generation remains largely unknown. Deletion of the C. elegans histone H3 lysine 4 dimethyl (H3K4me2) demethylase spr-5 leads to inherited accumulation of the euchromatic H3K4me2 mark and progressive decline in fertility. Here, we identified multiple chromatin-modifying factors, including H3K4me1/me2 and H3K9me3 methyltransferases, an H3K9me3 demethylase, and an H3K9me reader, which either suppress or accelerate the progressive transgenerational phenotypes of spr-5 mutant worms. Our findings uncover a network of chromatin regulators that control the transgenerational flow of epigenetic information and suggest that the balance between euchromatic H3K4 and heterochromatic H3K9 methylation regulates transgenerational effects on fertility.
authors.affiliation: Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA; Division of Newborn Medicine, Children's Hospital Boston, 300 Longwood Avenue, Boston, MA 02115, USA.
authors.forename: Eric L
authors.initials: EL
authors.lastname: Greer
authors.affiliation: Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: Sara E
authors.initials: SE
authors.lastname: Beese-Sims
authors.affiliation: Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA; Division of Newborn Medicine, Children's Hospital Boston, 300 Longwood Avenue, Boston, MA 02115, USA.
authors.forename: Emily
authors.initials: E
authors.lastname: Brookes
authors.affiliation: Division of Newborn Medicine, Children's Hospital Boston, 300 Longwood Avenue, Boston, MA 02115, USA.
authors.forename: Ruggero
authors.initials: R
authors.lastname: Spadafora
authors.affiliation: Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA 92093, USA.
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, The University of North Carolina at Chapel Hill School of Medicine, Chapel Hill, NC 27599, USA.
authors.forename: Scott B
authors.initials: SB
authors.lastname: Rothbart
authors.affiliation: Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA; Division of Newborn Medicine, Children's Hospital Boston, 300 Longwood Avenue, Boston, MA 02115, USA.
authors.forename: David
authors.initials: D
authors.lastname: Aristizábal-Corrales
authors.affiliation: Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA; Division of Newborn Medicine, Children's Hospital Boston, 300 Longwood Avenue, Boston, MA 02115, USA.
authors.forename: Shuzhen
authors.initials: S
authors.lastname: Chen
authors.affiliation: Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA; Division of Newborn Medicine, Children's Hospital Boston, 300 Longwood Avenue, Boston, MA 02115, USA.
authors.forename: Aimee I
authors.initials: AI
authors.lastname: Badeaux
authors.affiliation: Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA; Division of Newborn Medicine, Children's Hospital Boston, 300 Longwood Avenue, Boston, MA 02115, USA.
authors.forename: Qiuye
authors.initials: Q
authors.lastname: Jin
authors.affiliation: Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA 92093, USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, The University of North Carolina at Chapel Hill School of Medicine, Chapel Hill, NC 27599, USA.
authors.forename: Brian D
authors.initials: BD
authors.lastname: Strahl
authors.affiliation: Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: Monica P
authors.initials: MP
authors.lastname: Colaiácovo
authors.affiliation: Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA; Division of Newborn Medicine, Children's Hospital Boston, 300 Longwood Avenue, Boston, MA 02115, USA. Electronic address: yshi@hms.harvard.edu.
authors.forename: Yang
authors.initials: Y
authors.lastname: Shi
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics* / Q000378:metabolism; D029742:Caenorhabditis elegans Proteins / Q000235:genetics; D002843:Chromatin / Q000235:genetics / Q000378:metabolism; D044127:Epigenesis, Genetic; D057890:Epigenomics; D006657:Histones / Q000235:genetics* / Q000378:metabolism*; D008745:Methylation; D008780:Methyltransferases / Q000378:metabolism; D010089:Oxidoreductases, N-Demethylating / Q000235:genetics
chemical_list: D029742:Caenorhabditis elegans Proteins; D002843:Chromatin; D006657:Histones; D010089:Oxidoreductases, N-Demethylating; C468848:SPR-5 protein, C elegans; D008780:Methyltransferases