title: Disseminated tumor cells in bone marrow of gastric cancer patients: correlation with tumor hypoxia and clinical relevance.
abstract: Aim. The evaluation of the clinical relevance of disseminated tumor cells (DTCs) in bone marrow (BM) of patients with gastric cancer (GC) and their association with primary tumor hypoxia. Patients and Methods. 89 resected specimens were used. DTCs were detected using immunocytochemistry, the level of tumor hypoxia using NMR spectroscopy, CD68, CD34, VEGF, and VEGFR-1 (Flt-1) expression using immunohistochemistry, and MMP-2 and MMP-9 activity using zymography. Results. DTCs were detected in 51.4% of GC patients with M0. There was significant correlation between frequency of DTCs in BM and level of tumor hypoxia (P < 0.024). DTCs presence was accompanied with Flt-1 positivity of BM. The correlation between DTCs and tumor VEGF expression in patients with M0 was shown (P < 0.0248). Activity of MMP-2 and MMP-9 in BM was linked with DTCs in patients with M0 (P < 0.05). Overall survival (OS) of patients with M0 and DTCs was shorter than that of patients without DTCs (patients in both groups were operated only) (P = 0.0497). Conclusion. Appearance of DTCs correlates with hypoxia level in primary tumors. Detection of DTCs in GC patients may be relevant indicator for adjuvant chemotherapy using.
authors.affiliation: R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, National Academy of Sciences of Ukraine, Vasilkovskaya Street 45, Kiev 03022, Ukraine.
authors.forename: Larissa
authors.initials: L
authors.lastname: Bubnovskaya
authors.affiliation: R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, National Academy of Sciences of Ukraine, Vasilkovskaya Street 45, Kiev 03022, Ukraine.
authors.forename: Antonina
authors.initials: A
authors.lastname: Kovelskaya
authors.affiliation: R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, National Academy of Sciences of Ukraine, Vasilkovskaya Street 45, Kiev 03022, Ukraine.
authors.forename: Lilya
authors.initials: L
authors.lastname: Gumenyuk
authors.affiliation: R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, National Academy of Sciences of Ukraine, Vasilkovskaya Street 45, Kiev 03022, Ukraine.
authors.forename: Irina
authors.initials: I
authors.lastname: Ganusevich
authors.affiliation: R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, National Academy of Sciences of Ukraine, Vasilkovskaya Street 45, Kiev 03022, Ukraine.
authors.forename: Lesya
authors.initials: L
authors.lastname: Mamontova
authors.affiliation: R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, National Academy of Sciences of Ukraine, Vasilkovskaya Street 45, Kiev 03022, Ukraine.
authors.forename: Victor
authors.initials: V
authors.lastname: Mikhailenko
authors.affiliation: City Clinical Oncological Center, Verchovynna Street 69, Kiev 03115, Ukraine.
authors.forename: Dmitry
authors.initials: D
authors.lastname: Osinsky
authors.affiliation: City Clinical Oncological Center, Verchovynna Street 69, Kiev 03115, Ukraine.
authors.forename: Sergej
authors.initials: S
authors.lastname: Merentsev
authors.affiliation: R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, National Academy of Sciences of Ukraine, Vasilkovskaya Street 45, Kiev 03022, Ukraine.
authors.forename: Sergej
authors.initials: S
authors.lastname: Osinsky