title: Sulfated modification of longan polysaccharide and its immunomodulatory and antitumor activity in vitro.
abstract: A water-soluble polysaccharide fraction (LP1) was prepared from Dimocarpus longan Lour. by hot water extraction, DEAE-cellulose and Sephadex G-100 chromatography. Its sulfated derivative (LP1-S) was prepared by the sulfuric acid method. Preliminary tests in vitro showed LP1 and LP1-S could stimulate murine lymphocytes proliferation, increase pinocytic activity of murine macrophages and production of nitric oxide (NO), interleukin 6 (IL-6), IL-1β and tumor necrosis factor-alpha (TNF-α) in macrophages. Furthermore, LP1-S exhibited higher antiproliferative activity against human nasopharyngeal carcinoma HONE1 cells in vitro than LP1, which might be caused by the sulfate group in its structures. These results indicated that the LP1-S might be useful for developing safe antitumor drugs or health food.
authors.affiliation: College of Pharmaceutical Sciences, Guangxi Medical University, Nanning 530021, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Jiang
authors.affiliation: College of Pharmaceutical Sciences, Guangxi Medical University, Nanning 530021, PR China.
authors.forename: Fa-Yan
authors.initials: FY
authors.lastname: Meng
authors.affiliation: Guang Xi Zhuang Autonomous Region People's Hospital, Nanning 530021, PR China.
authors.forename: Zhou
authors.initials: Z
authors.lastname: He
authors.affiliation: College of Pharmaceutical Sciences, Guangxi Medical University, Nanning 530021, PR China.
authors.forename: Yuan-Ling
authors.initials: YL
authors.lastname: Ning
authors.affiliation: College of Pharmaceutical Sciences, Guangxi Medical University, Nanning 530021, PR China. Electronic address: onlythankforyou@163.com.
authors.forename: Xue-Hua
authors.initials: XH
authors.lastname: Li
authors.affiliation: College of Pharmaceutical Sciences, Guangxi Medical University, Nanning 530021, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Song
authors.affiliation: College of Pharmaceutical Sciences, Guangxi Medical University, Nanning 530021, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Pharmaceutical Sciences, Guangxi Medical University, Nanning 530021, PR China.
authors.forename: Rui
authors.initials: R
authors.lastname: Zhou
mesh_terms: D000818:Animals; D002277:Carcinoma; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D051379:Mice; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000188:drug therapy*; D009569:Nitric Oxide / Q000096:biosynthesis; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry*; D011134:Polysaccharides / Q000008:administration & dosage / Q000737:chemistry*; D029631:Sapindaceae / Q000737:chemistry; D013431:Sulfates / Q000737:chemistry; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D010936:Plant Extracts; D011134:Polysaccharides; D013431:Sulfates; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide
keywords: Antitumor activity; Dimocarpus longan; Immunomodulation; Polysaccharide; Sulfated modification