title: Interleukin-1 receptors are differentially expressed in normal and psoriatic T cells.
abstract: This study was carried out to examine the possible role of interleukin-1 (IL-1) in the functional insufficiency of regulatory T cells in psoriasis, by comparing the expression of IL-1 receptors on healthy control and psoriatic T cells. Patients with moderate-to-severe chronic plaque psoriasis and healthy volunteers, matched in age and sex, were selected for all experiments. CD4(+)CD25(-) effector and CD4(+)CD25(+)CD127(low) regulatory T cells were separated and used for the experiments. Expression of the mRNA of IL-1 receptors (IL-1R1, IL-1R2, and sIL-1R2) was determined by quantitative real-time RT-PCR. Cell surface IL-1 receptor expression was assessed by flow cytometry. Relative expression of the signal transmitting IL-1 receptor type 1 (IL-1R1) mRNA is higher in resting psoriatic effector and regulatory T cells, and activation induces higher IL-1R1 protein expression in psoriatic T cells than in healthy cells. Psoriatic regulatory and effector T cells express increased mRNA levels of the decoy IL-1 receptors (IL-1R2 and sIL-1R2) upon activation compared to healthy counterparts. Psoriatic T cells release slightly more sIL-1R2 into their surrounding than healthy T cells. In conclusion, changes in the expression of IL-1 receptors in psoriatic regulatory and effector T cells could contribute to the pathogenesis of psoriasis.
authors.affiliation: Department of Dermatology and Allergology, University of Szeged, Korányi fasor 6, Szeged 6720, Hungary.
authors.forename: Attila
authors.identifier: 0000-0002-8316-5622
authors.initials: A
authors.lastname: Bebes
authors.affiliation: Department of Dermatology and Allergology, University of Szeged, Korányi fasor 6, Szeged 6720, Hungary.
authors.forename: Ferenc
authors.initials: F
authors.lastname: Kovács-Sólyom
authors.affiliation: Department of Dermatology and Allergology, University of Szeged, Korányi fasor 6, Szeged 6720, Hungary.
authors.forename: Judit
authors.initials: J
authors.lastname: Prihoda
authors.affiliation: Department of Dermatology and Allergology, University of Szeged, Korányi fasor 6, Szeged 6720, Hungary.
authors.forename: Róbert
authors.initials: R
authors.lastname: Kui
authors.affiliation: Department of Dermatology and Allergology, University of Szeged, Korányi fasor 6, Szeged 6720, Hungary ; Dermatological Research Group of the Hungarian Academy of Sciences, University of Szeged, Korányi fasor 6, Szeged 6720, Hungary.
authors.forename: Lajos
authors.initials: L
authors.lastname: Kemény
authors.affiliation: Department of Dermatology and Allergology, University of Szeged, Korányi fasor 6, Szeged 6720, Hungary ; Department of Dermatology, Venereology and Oncodermatology, University of Pécs, Kodály Z. u. 20, Pécs 7624, Hungary.
authors.forename: Rolland
authors.initials: R
authors.lastname: Gyulai
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000166:cytology*; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005786:Gene Expression Regulation; D006801:Humans; D007249:Inflammation; D053645:Interleukin-2 Receptor alpha Subunit / Q000378:metabolism; D053699:Interleukin-7 Receptor alpha Subunit / Q000378:metabolism; D011565:Psoriasis / Q000276:immunology / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D017472:Receptors, Interleukin-1 / Q000235:genetics* / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D053645:Interleukin-2 Receptor alpha Subunit; D053699:Interleukin-7 Receptor alpha Subunit; D012333:RNA, Messenger; D017472:Receptors, Interleukin-1