title: Early life perfluorooctanesulphonic acid (PFOS) exposure impairs zebrafish organogenesis.
abstract: As a persistent organic contaminant, perfluorooctanesulphonic acid (PFOS) has been widely detected in the environment, wildlife, and humans. The present study revealed that zebrafish embryos exposed to 16 μM PFOS during a sensitive window of 48-96 hour post-fertilization (hpf) disrupted larval morphology at 120 hpf. Malformed zebrafish larvae were characterized by uninflated swim bladder, less developed gut, and curved spine. Histological and ultrastructural examination of PFOS-exposed larvae showed structural alterations in swim bladder and gut. Whole genome microarray was used to identify the early transcripts dysregulated following exposure to 16 μM PFOS at 96 hpf. In total, 1278 transcripts were significantly misexpressed (p<0.05) and 211 genes were changed at least two-fold upon PFOS exposure in comparison to the vehicle-exposed control group. A PFOS-induced network of perturbed transcripts relating to swim bladder and gut development revealed that misexpression of genes were involved in organogenesis. Taken together, early life stage exposure to PFOS perturbs various molecular pathways potentially resulting in observed defects in swim bladder and gut development.
authors.affiliation: Zhejiang Provincial Key Laboratory for Technology and Application of Model Organisms; Institute of Environmental Safety and Human Health, Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Jiangfei
authors.initials: J
authors.lastname: Chen
authors.affiliation: Environmental and Molecular Toxicology, The Sinnhuber Aquatic Research Laboratory and the Environmental Health Sciences Center, Oregon State University, Corvallis, OR 97333, USA.
authors.forename: Robert L
authors.initials: RL
authors.lastname: Tanguay
authors.affiliation: Environmental and Molecular Toxicology, The Sinnhuber Aquatic Research Laboratory and the Environmental Health Sciences Center, Oregon State University, Corvallis, OR 97333, USA.
authors.forename: Tamara L
authors.initials: TL
authors.lastname: Tal
authors.affiliation: Zhejiang Provincial Key Laboratory for Technology and Application of Model Organisms; Institute of Environmental Safety and Human Health, Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Zengxin
authors.initials: Z
authors.lastname: Gai
authors.affiliation: Zhejiang Provincial Key Laboratory for Technology and Application of Model Organisms; Institute of Environmental Safety and Human Health, Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Ma
authors.affiliation: Zhejiang Provincial Key Laboratory for Technology and Application of Model Organisms; Institute of Environmental Safety and Human Health, Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Chenglian
authors.initials: C
authors.lastname: Bai
authors.affiliation: Computational Biology and Bioinformatics, Pacific Northwest National Laboratory, Richland, WA 99352, USA.
authors.forename: Susan C
authors.initials: SC
authors.lastname: Tilton
authors.affiliation: Zhejiang Provincial Key Laboratory for Technology and Application of Model Organisms; Institute of Environmental Safety and Human Health, Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Daqing
authors.initials: D
authors.lastname: Jin
authors.affiliation: Zhejiang Provincial Key Laboratory for Technology and Application of Model Organisms; Institute of Environmental Safety and Human Health, Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Dongren
authors.initials: D
authors.lastname: Yang
authors.affiliation: Zhejiang Provincial Key Laboratory for Technology and Application of Model Organisms; Institute of Environmental Safety and Human Health, Wenzhou Medical University, Wenzhou 325035, China. Electronic address: cjhuang5711@163.com.
authors.forename: Changjiang
authors.initials: C
authors.lastname: Huang
authors.affiliation: Zhejiang Provincial Key Laboratory for Technology and Application of Model Organisms; Institute of Environmental Safety and Human Health, Wenzhou Medical University, Wenzhou 325035, China. Electronic address: dqxdong@163.com.
authors.forename: Qiaoxiang
authors.initials: Q
authors.lastname: Dong
mesh_terms: D000400:Air Sacs / Q000196:embryology; D017738:Alkanesulfonic Acids / Q000633:toxicity*; D000818:Animals; D004625:Embryo, Nonmammalian; D004781:Environmental Exposure; D005466:Fluorocarbons / Q000633:toxicity*; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D007422:Intestines / Q000196:embryology; D038081:Organogenesis / Q000187:drug effects*; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D015027:Zebrafish / Q000196:embryology / Q000235:genetics
chemical_list: D017738:Alkanesulfonic Acids; D005466:Fluorocarbons; D014874:Water Pollutants, Chemical; C076994:perfluorooctane sulfonic acid
keywords: Developmental toxicity; Gut; Perfluorooctanesulfonic acid; Swim bladder; Zebrafish embryo