title: Distribution and quantitative detection of GABAA receptor in Carassius auratus gibelio.
abstract: Gamma-aminobutyric acid (GABA), a major inhibitory neurotransmitter in brain, is synthesized from glutamate and metabolized to succinic semialdehyde by glutamic acid decarboxylase (GAD) and GABA transaminase (GABA-T), respectively. The fast inhibitory effect of GABA is mediated by GABA type A (GABAA) receptors that are associated with several neurological disorders, and GABAA receptors are targets of several therapeutic agents. To date, information on the distribution and quantity of GABAA receptors in Carassius auratus gibelio is still limited. We investigated for the first time, the tissue-specific distribution of GABAARβ2a and GABAARβ2b, the two subunits of the predominant GABAA receptor subtype (α1β2γ2), and then, the expression of GABAARβ2a, GABAARβ2b, GAD, and quantified GABA-T genes in different tissues by quantitative real-time PCR method and compared different expressions between two developmental stages of C. auratus gibelio. Results showed that GABAARβ2a and GABAARβ2b genes expressed in both brain and peripheral organs using reverse transcription-polymerase chain reaction. In addition, the majority of GABAARβ2a, GABAARβ2b, GAD, and GABA-T were mainly synthesized in brain; however, a considerable amount of GABA-T was secreted from the peripheral tissues, especially in the liver. Moreover, the expression of GABAARβ2a and GABAARβ2b genes in different tissues varied with body weight change. This study provides a reference for further studies on GABA and GABAA receptors subunits and an insight on the possible pharmacological properties of the GABAA receptor in C. auratus gibelio.
authors.affiliation: National Center for Aquatic Pathogen Collection, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, People's Republic of China.
authors.forename: Jiming
authors.initials: J
authors.lastname: Ruan
authors.forename: Kun
authors.initials: K
authors.lastname: Hu
authors.forename: Haixin
authors.initials: H
authors.lastname: Zhang
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.forename: Ailing
authors.initials: A
authors.lastname: Zhou
authors.forename: Yini
authors.initials: Y
authors.lastname: Zhao
authors.forename: Xianle
authors.initials: X
authors.lastname: Yang
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001835:Body Weight; D001921:Brain / Q000378:metabolism*; D017931:DNA Primers / Q000235:genetics; D006054:Goldfish / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D009928:Organ Specificity; D020033:Protein Isoforms / Q000378:metabolism; D011963:Receptors, GABA-A / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D020033:Protein Isoforms; D011963:Receptors, GABA-A