title: Biofilms formed by Mycobacterium tuberculosis on cement, ceramic, and stainless steel surfaces and their controls.
abstract: This study assessed the biofilms formed by selected strains of Mycobacterium tuberculosis and investigated the efficacy of three different treatments to control the biofilms. Two M. tuberculosis strains were inoculated separately in 150 ml of Middlebrook 7H9-Tween 80 (0.1%) broth with 5% liver extract and 10% oleic albumin dextrose catalase (OADC) supplement, 5% liver extract alone, or 10% OADC alone in sterile jars, each containing a 2-cm2 coupon of cement, ceramic, or stainless steel for biofilm development at 37 °C, with agitation for 2, 3, or 4 weeks. Biofilms on the coupons were exposed to 10 ml of 2% sanitizer A or 0.5% sanitizer B at 28 and 45 °C and to hot water at 85 °C for 5 min. Residual biofilms on treated and untreated coupons were assessed. Both strains of M. tuberculosis formed biofilms on the three surfaces; however, one strain formed more biofilms. More biofilms were formed when media containing 5% liver extract was used. Biofilm mass increased as incubation time increased until the third week. More biofilms were formed on cement than on ceramic and stainless steel coupons. Sanitizing treatments at 45 °C removed more biofilms than those at 28 °C. However, neither treatment completely eliminated the biofilms.
authors.affiliation: Department of Veterinary Public Health and Preventive Medicine, College of Medicine, University College Hospital, University of Ibadan, Ibadan, 200284 Nigeria.
authors.forename: Victoria
authors.initials: V
authors.lastname: Adetunji
authors.affiliation: Department of Medical Microbiology and Parasitology, College of Medicine, University College Hospital, University of Ibadan, Ibadan, 200284 Nigeria.
authors.forename: Aderemi
authors.initials: A
authors.lastname: Kehinde
authors.affiliation: Department of Veterinary Public Health and Preventive Medicine, College of Medicine, University College Hospital, University of Ibadan, Ibadan, 200284 Nigeria.
authors.forename: Olayemi
authors.initials: O
authors.lastname: Bolatito
authors.affiliation: Department of Food Science and Technology, The University of Georgia, Griffin, Georgia 30223-1797, USA. jchen@uga.edu.
authors.forename: Jinru
authors.initials: J
authors.lastname: Chen
mesh_terms: D018441:Biofilms / Q000254:growth & development*; D002516:Ceramics; D015169:Colony Count, Microbial; D004866:Equipment Contamination; D005506:Food Contamination / Q000032:analysis / Q000517:prevention & control; D005516:Food Microbiology; D009169:Mycobacterium tuberculosis / Q000502:physiology*; D013193:Stainless Steel; D013696:Temperature; D013997:Time Factors
chemical_list: D013193:Stainless Steel