title: Micromechanical PDGF recognition via lab-on-a-disc aptasensor arrays.
abstract: A plug-and-play CD-like platform is used to perform a statistical detection of platelet derived growth factor (PDGF) proteins through aptamer-based surface functionalization of multiple microcantilever arrays. When PDGF proteins bind to aptamer coatings, the cantilevers deflect. The deflection response is monitored by optical read-out units from a commercial DVD-ROM device. We report on the use of an improved sensing platform which facilitates measurements under continuous liquid flow and with temperature control. Also, the mechanical wobbling of the DVD-ROM platform has been minimized and the scanning system has been optimized in order to detect cantilever deflections in liquid with nanometer scale resolution. The capability of the sensing platform is demonstrated by detection of clinically relevant concentrations of PDGF proteins. We present statistical measurements on 100 microcantilevers at different concentrations of PDGF, ranging from 10 nM to 400 nM. Hereby it is possible to reliably characterize the averaged mechanical response of cantilevers as a function of protein concentration.
authors.affiliation: Department of Micro- and Nanotechnology, Technical University of Denmark, Lyngby 2800, Denmark.
authors.forename: F G
authors.initials: FG
authors.lastname: Bosco
authors.affiliation: Department of Micro- and Nanotechnology, Technical University of Denmark, Lyngby 2800, Denmark.
authors.forename: M
authors.initials: M
authors.lastname: Bache
authors.affiliation: Department of Mechanical Engineering, Columbia University, New York 10027, NY, United States.
authors.forename: J
authors.initials: J
authors.lastname: Yang
authors.affiliation: Institute of Physics, Academia Sinica, Nankang, Taipei 11529, Taiwan.
authors.forename: C H
authors.initials: CH
authors.lastname: Chen
authors.affiliation: Institute of Physics, Academia Sinica, Nankang, Taipei 11529, Taiwan.
authors.forename: E-T
authors.initials: ET
authors.lastname: Hwu
authors.affiliation: Department of Mechanical Engineering, Columbia University, New York 10027, NY, United States.
authors.forename: Q
authors.initials: Q
authors.lastname: Lin
authors.affiliation: Department of Micro- and Nanotechnology, Technical University of Denmark, Lyngby 2800, Denmark.
authors.forename: A
authors.initials: A
authors.lastname: Boisen
keywords: Biosensing; Cantilevers; DVD-ROM; Lab-on-a-disc; Platelet derived growth factor; Protein detection