title: Using immunomic approach to enhance tumor-associated autoantibody detection in diagnosis of hepatocellular carcinoma.
abstract: To explore the possibility of using a mini-array of multiple tumor-associated antigens (TAAs) as an approach to the diagnosis of hepatocellular carcinoma (HCC), 14 TAAs were selected to examine autoantibodies in sera from patients with chronic hepatitis, liver cirrhosis and HCC by immunoassays. Antibody frequency to any individual TAA in HCC varied from 6.6% to 21.1%. With the successive addition of TAAs to the panel of TAAs, there was a stepwise increase of positive antibody reactions. The sensitivity and specificity of 14 TAAs for immunodiagnosis of HCC was 69.7% and 83.0%, respectively. This TAA mini-array also identified 43.8% of HCC patients who had normal alpha-fetoprotein (AFP) levels in serum. In summary, this study further supports the hypothesis that a customized TAA array used for detecting anti-TAA autoantibodies can constitute a promising and powerful tool for immunodiagnosis of HCC and may be especially useful in patients with normal AFP levels.
authors.affiliation: Department of Biological Sciences, The University of Texas at El Paso, El Paso, TX 79968, USA.
authors.forename: Liping
authors.initials: L
authors.lastname: Dai
authors.affiliation: Department of Biological Sciences, The University of Texas at El Paso, El Paso, TX 79968, USA.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Ren
authors.affiliation: Department of Biological Sciences, The University of Texas at El Paso, El Paso, TX 79968, USA.
authors.forename: Mei
authors.initials: M
authors.lastname: Liu
authors.affiliation: The Scripps Research Institute, La Jolla, CA 92037, USA.
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Imai
authors.affiliation: The Scripps Research Institute, La Jolla, CA 92037, USA.
authors.forename: Eng M
authors.initials: EM
authors.lastname: Tan
authors.affiliation: Department of Biological Sciences, The University of Texas at El Paso, El Paso, TX 79968, USA. Electronic address: jzhang@utep.edu.
authors.forename: Jian-Ying
authors.initials: JY
authors.lastname: Zhang
mesh_terms: D000912:Antibodies, Neoplasm / Q000097:blood* / Q000276:immunology; D000951:Antigens, Neoplasm / Q000276:immunology*; D001323:Autoantibodies / Q000097:blood* / Q000276:immunology; D014408:Biomarkers, Tumor / Q000097:blood* / Q000276:immunology; D006528:Carcinoma, Hepatocellular / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D005260:Female; D006521:Hepatitis, Chronic / Q000097:blood / Q000276:immunology; D006801:Humans; D007159:Immunologic Tests / Q000379:methods; D008103:Liver Cirrhosis / Q000097:blood / Q000276:immunology; D008113:Liver Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D011994:Recombinant Proteins / Q000096:biosynthesis; D000509:alpha-Fetoproteins / Q000378:metabolism
chemical_list: C097353:AFP protein, human; D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D001323:Autoantibodies; D014408:Biomarkers, Tumor; D011994:Recombinant Proteins; D000509:alpha-Fetoproteins
keywords: Autoantibodies;; Cancer immunodiagnosis;; Hepatocellular carcinoma; Tumor-associated antigens (TAAs);