title: The use of amphipols for solution NMR studies of membrane proteins: advantages and constraints as compared to other solubilizing media.
abstract: Solution-state nuclear magnetic resonance studies of membrane proteins are facilitated by the increased stability that trapping with amphipols confers to most of them as compared to detergent solutions. They have yielded information on the state of folding of the proteins, their areas of contact with the polymer, their dynamics, water accessibility, and the structure of protein-bound ligands. They benefit from the diversification of amphipol chemical structures and the availability of deuterated amphipols. The advantages and constraints of working with amphipols are discussed and compared to those associated with other non-conventional environments, such as bicelles and nanodiscs.
authors.affiliation: Laboratoire de Biologie Physico-Chimique des Protéines Membranaires, Institut de Biologie Physico-Chimique (FRC 550), UMR 7099, CNRS, Université Paris 7, 13 rue Pierre et Marie Curie, 75005, Paris, France.
authors.forename: Noelya
authors.initials: N
authors.lastname: Planchard
authors.forename: Élodie
authors.initials: É
authors.lastname: Point
authors.forename: Tassadite
authors.initials: T
authors.lastname: Dahmane
authors.forename: Fabrice
authors.initials: F
authors.lastname: Giusti
authors.forename: Marie
authors.initials: M
authors.lastname: Renault
authors.forename: Christel
authors.initials: C
authors.lastname: Le Bon
authors.forename: Grégory
authors.initials: G
authors.lastname: Durand
authors.forename: Alain
authors.initials: A
authors.lastname: Milon
authors.forename: Éric
authors.initials: É
authors.lastname: Guittet
authors.forename: Manuela
authors.initials: M
authors.lastname: Zoonens
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Popot
authors.forename: Laurent J
authors.initials: LJ
authors.lastname: Catoire
mesh_terms: D000818:Animals; D016477:Artifacts; D002462:Cell Membrane / Q000737:chemistry*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008051:Lipid Bilayers / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008565:Membrane Proteins / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D012995:Solubility; D012996:Solutions; D013501:Surface-Active Agents / Q000737:chemistry*; D013673:Technology Assessment, Biomedical; D014867:Water / Q000737:chemistry
chemical_list: D008051:Lipid Bilayers; D008565:Membrane Proteins; D011108:Polymers; D012996:Solutions; D013501:Surface-Active Agents; D014867:Water