title: The effect of variants in the promoter of BMPER on the intramuscular fat deposition in longissimus dorsi muscle of pigs.
abstract: The aim of the study was to evaluate the contribution of BMPER promoter SNPs to the gene expression and intramuscular fat content in longissimus dorsi muscle. Firstly the promoter region of BMPER was comparatively scanned by direct sequencing with pool DNA of two groups (n=15 for each group) with high (H) or low (L) IMF content. Two SNPs, c.-1423A>G and c.-1344A>C, were found to have reverse allele distribution in the two groups. Genotyping by PCR-SSCP in a larger population revealed that the two SNPs interlock completely to form only A-A or G-C haplotype. The IMF content and BMPER expression level of A-A/A-A genotype were higher than G-C/G-C genotype, and luciferase assay revealed that A-A haplotype promoter activity was also higher than G-C haplotype. Putative transcription factor prediction suggested that c.-1344 A>C mutation might shift the promoter binding affinity with GATAs. We concluded that BMPER promoter polymorphisms have an effect on IMF content, and A-A haplotype could be used as a candidate genetic marker for preferable IMF deposition.
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Liu
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Wenxing
authors.initials: W
authors.lastname: Sun
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Yongyan
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Chunying
authors.initials: C
authors.lastname: Xu
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Fu
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China. Electronic address: jiechen@njau.edu.cn.
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D050980:GATA Transcription Factors / Q000235:genetics / Q000378:metabolism; D006239:Haplotypes; D018482:Muscle, Skeletal / Q000033:anatomy & histology / Q000502:physiology; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D034421:Sus scrofa / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D050980:GATA Transcription Factors; D014157:Transcription Factors
keywords: BMPER; IMF; Promoter; SNP; Transcriptional activity