title: IL-17 promotes Type 1 T cell response through modulating dendritic cell function in acute allograft rejection.
abstract: IL-17 is a cytokine that produced by various type of cell. Previous studies have been shown that IL-17 plays a critical role in the pathogenesis of different diseases. However, few studies have addressed the source and mechanism of IL-17 in the development of allograft rejection response. In this study, we present that the IL-17 expression reaches the strongest response at the early stage of cardiac allograft rejection, and was elevated earlier than DC maturation. The IL-17 is predominantly produced by CD3(+) T cells, whereas CD11c, CD11b, and NK1.1 positive cells rarely expressed IL-17. It is worth noting that blockade of endogenous IL-17 activity suppressed DC maturation, decreased inflammatory cytokines and impaired Th1 immune response during acute allograft rejection. Furthermore, adoptive transfer with DCs from IL-17-treated mice had a significant longer allograft survival time and decreased number of IFN-γ produced by T cells. Consistently, in an in vitro experiment, recombinant IL-17 significantly up-regulate co-stimulatory molecules of bone marrow derived dendritic cells (BMDCs), and IL-17-treated BMDCs show that an increased capacity to enhance T cell function was also observed. In conclusion, our data provide clear evidence that the early elevated level of IL-17 contributes to allograft rejection through modulating dendritic cell function.
authors.affiliation: Department of Rheumatology and Clinical Immunology, The First Hospital of Xiamen University, Xiamen, China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Duan
authors.affiliation: Basic Medical Department of Medical College, Xiamen University, Xiamen, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Immunology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Quansong
authors.initials: Q
authors.lastname: Xia
authors.affiliation: Department of Rheumatology and Clinical Immunology, The First Hospital of Xiamen University, Xiamen, China.
authors.forename: Liying
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Thoracic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Fan
authors.affiliation: Department of Rheumatology and Clinical Immunology, The First Hospital of Xiamen University, Xiamen, China.
authors.forename: K R
authors.initials: KR
authors.lastname: Sigdel
authors.affiliation: Department of Immunology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Min
authors.initials: M
authors.lastname: Fang
authors.affiliation: Department of Immunology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Zheng
authors.affiliation: Department of Rheumatology and Clinical Immunology, The First Hospital of Xiamen University, Xiamen, China. Electronic address: guixiu.shi@gmail.com.
authors.forename: Guixiu
authors.initials: G
authors.lastname: Shi
authors.affiliation: Department of Immunology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. Electronic address: flgong@163.com.
authors.forename: Feili
authors.initials: F
authors.lastname: Gong
mesh_terms: D019264:Adoptive Transfer; D064591:Allografts / Q000276:immunology* / Q000637:transplantation; D000818:Animals; D019138:Antibodies, Blocking / Q000008:administration & dosage; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology* / Q000637:transplantation; D006084:Graft Rejection / Q000188:drug therapy / Q000276:immunology*; D016027:Heart Transplantation; D007371:Interferon-gamma / Q000378:metabolism; D020381:Interleukin-17 / Q000276:immunology*; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018417:Th1 Cells / Q000276:immunology*; D015854:Up-Regulation
chemical_list: D019138:Antibodies, Blocking; D015703:Antigens, CD; D020381:Interleukin-17; D007371:Interferon-gamma
keywords: Allograft rejection; DC; IL-17