title: Accuracy of capillary blood 3-β-hydroxybutyrate determination for the detection and treatment of canine diabetic ketoacidosis.
abstract: In human medicine, diagnosis of diabetic ketoacidosis (DKA) is usually based on measurement of capillary 3-β-hydroxybutyrate (3-HB) with a hand held ketone sensor. This study was conducted to determine if measurement of capillary 3-HB could be useful for the diagnosis and monitoring of canine DKA. Fifteen dogs with diabetic ketosis and 10 with DKA were evaluated. Paired measurements of 3-HB of capillary and venous blood samples were analysed by the electrochemical sensor and reference method. Use of capillary 3-HB measurement during DKA management was then evaluated through simultaneous measurements of capillary 3-HB, urinary AcAc and venous blood gas analysis. Good agreement between capillary and venous 3-HB measurement was detected by the electrochemical sensor and reference method. Monitoring treatment of DKA revealed a significant correlation between capillary 3-HB and acidosis markers, while no significant correlation was observed between AcAc and acidosis markers. A cut-off value of capillary blood 3-HB > 3.8 mmol/L for diagnosis of DKA resulted in 70% and 92% sensitivity and specificity. The electrochemical sensor accurately measures 3-HB concentration in both capillary and venous blood samples, is accurate in diagnosing canine DKA, and appears to reflect the patient's metabolic status during DKA treatment.
authors.affiliation: Department of Veterinary Medical Sciences, University of Bologna, 40126 Bologna, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Bresciani
authors.forename: Marco
authors.initials: M
authors.lastname: Pietra
authors.forename: Sara
authors.initials: S
authors.lastname: Corradini
authors.forename: Massimo
authors.initials: M
authors.lastname: Giunti
authors.forename: Federico
authors.initials: F
authors.lastname: Fracassi
mesh_terms: D020155:3-Hydroxybutyric Acid / Q000097:blood; D000818:Animals; D001774:Blood Chemical Analysis / Q000592:standards / Q000662:veterinary*; D001800:Blood Specimen Collection / Q000295:instrumentation / Q000662:veterinary*; D002196:Capillaries / Q000737:chemistry; D016883:Diabetic Ketoacidosis / Q000175:diagnosis / Q000628:therapy / Q000662:veterinary*; D004283:Dog Diseases / Q000175:diagnosis* / Q000628:therapy; D004285:Dogs; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000662:veterinary*
chemical_list: D020155:3-Hydroxybutyric Acid
keywords: acetoacetate; diabetes mellitus; dog; ketone bodies