title: Increased DNase I activity in diabetes might be associated with injury of pancreas.
abstract: DNase I is an endonuclease responsible to destruction of chromatin during apoptosis. However, its role in diabetes is still unclear. With blood samples from our previous study related to type 2 diabetes, we examined the DNase I activity in the serum of these patients and the role of DNase I in the injury of pancreas was further investigated in rats and INS-1 cells. Serum and pancreatic tissues from human and rats were used for the study. Insulin resistance and diabetes were induced by high fat diet and STZ injection, respectively. DNase I activity was determined by radial enzyme-diffusion method. Expressions of DNase I and caspase-3 in pancreas were determined in rat pancreatic tissues and INS-1 cells. Apoptosis of INS-1 cells was determined by both TUNEL assay and Flow Cytometry. There was a significant elevation of DNase I activity in serum of patients with type 2 diabetes and rats with STZ injection. Moreover, increase in DNase I expression was observed in the pancreas of diabetic person and rats. Furthermore, high glucose induced both DNase I and caspase-3 expression and at the same time increased apoptosis rate of INS-1 cells. In conclusion, elevated DNase I in diabetes may be related to pancreatic injury and could be one of the causes that induce diabetes.
authors.affiliation: Graduate School of Peking Union Medical College, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhu
authors.forename: Yuewen
authors.initials: Y
authors.lastname: Gong
authors.forename: Pengmin
authors.initials: P
authors.lastname: Chen
authors.forename: Haojun
authors.initials: H
authors.lastname: Zhang
authors.forename: Tingting
authors.initials: T
authors.lastname: Zhao
authors.forename: Ping
authors.initials: P
authors.lastname: Li
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics; D003850:Deoxyribonuclease I / Q000097:blood*; D003921:Diabetes Mellitus, Experimental / Q000097:blood* / Q000139:chemically induced / Q000201:enzymology / Q000473:pathology; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000201:enzymology / Q000473:pathology; D059305:Diet, High-Fat; D006801:Humans; D007333:Insulin Resistance / Q000235:genetics; D050417:Insulin-Secreting Cells; D010179:Pancreas / Q000201:enzymology* / Q000293:injuries / Q000473:pathology; D051381:Rats; D013311:Streptozocin / Q000633:toxicity
chemical_list: D013311:Streptozocin; D003850:Deoxyribonuclease I