title: Mangromicins, six new anti-oxidative agents isolated from a culture broth of the actinomycete, Lechevalieria aerocolonigenes K10-0216.
abstract: We have been continually searching for novel chemical compounds from culture broths of various actinomycetes using a physicochemical screening system. During the course of this program, we have previously reported the discovery of two new natural products, designated mangromicins A and B, discovered in a broth of a rare actinomycete strain, Lechevalieria aerocolonigenes K10-0216. Mangromicins have a unique and rare structure, a cyclopentadecane skeleton with a tetrahydrofuran unit and a 5,6-dihydro-4-hydroxy-2-pyrone moiety. New mangromicin analogs were isolated by using an improved production medium. As a consequence, six analogs, together with mangromicins A and B, were isolated from a cultured broth of L. aerocolonigenes K10-0216. We named them mangromicins D, E, F, G, H and I. All mangromicins showed radical scavenging activities against 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radicals and nitric oxide generated from LPS-stimulated RAW264.7 cells, a murine macrophage cell line. Among the analogs, mangromicins A and I showed the most potent DPPH radical scavenging activity and nitric oxide scavenging activity, respectively.
authors.affiliation: Research Organization for Infection Control Sciences, Kitasato University, Tokyo, Japan.
authors.forename: Takuji
authors.initials: T
authors.lastname: Nakashima
authors.affiliation: Graduate School of Infection Control Sciences, Kitasato University, Tokyo, Japan.
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Kamiya
authors.affiliation: 1] Graduate School of Infection Control Sciences, Kitasato University, Tokyo, Japan [2] Kitasato Institute for Life Sciences, Kitasato University, Tokyo, Japan.
authors.forename: Masato
authors.initials: M
authors.lastname: Iwatsuki
authors.affiliation: 1] Graduate School of Infection Control Sciences, Kitasato University, Tokyo, Japan [2] Kitasato Institute for Life Sciences, Kitasato University, Tokyo, Japan.
authors.forename: Yōko
authors.initials: Y
authors.lastname: Takahashi
authors.affiliation: Kitasato Institute for Life Sciences, Kitasato University, Tokyo, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Ōmura
mesh_terms: D039903:Actinobacteria / Q000737:chemistry*; D000818:Animals; D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology*; D002460:Cell Line; D016166:Free Radical Scavengers / Q000737:chemistry* / Q000494:pharmacology; D005663:Furans / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects; D051379:Mice; D009569:Nitric Oxide / Q000737:chemistry; D011753:Pyrones / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D005663:Furans; D011753:Pyrones; D009569:Nitric Oxide; C018674:tetrahydrofuran