title: IL2/IL-4, OX40L and FDC-like cell line support the in vitro tumor cell growth of adult T-cell leukemia/lymphoma.
abstract: Adult T-cell leukemia/lymphoma (ATLL) is a peripheral T-cell neoplasm with an extremely poor prognosis. Maintaining ATLL cells in vitro is difficult and little is known about how they maintain themselves or grow in patients. Elucidating the interaction between ATLL cells and surrounding host factors might therefore provide important insights into pathophysiology. We cultured primary ATLL cells in various culture conditions using IL-2, IL-4 and feeder cells, and established two cell lines dependent on IL-2, IL-4 and a follicular dendritic cell-derived cell line, HK, in which OX40-ligand was induced. Our study indicates the importance of microenvironment in the homeostasis of ATLL.
authors.affiliation: Division of Epidemiology and Prevention, Aichi Cancer Center Research Institute, Nagoya, Aichi, Japan.
authors.forename: Dai
authors.initials: D
authors.lastname: Chihara
authors.affiliation: Department of Hematology, Toyota Kosei Hospital, Toyota, Japan. Electronic address: y-kagami@toyota.jaaikosei.or.jp.
authors.forename: Yoshitoyo
authors.initials: Y
authors.lastname: Kagami
authors.affiliation: Department of Haematology and Cell Therapy, Aichi Cancer Center Hospital, Nagoya, Aichi, Japan.
authors.forename: Harumi
authors.initials: H
authors.lastname: Kato
authors.affiliation: Division of Molecular Medicine, Aichi Cancer Center Research Institute, Nagoya, Aichi, Japan.
authors.forename: Noriaki
authors.initials: N
authors.lastname: Yoshida
authors.affiliation: Division of Virology, National Cancer Center Research Institute, Tokyo, Japan.
authors.forename: Tohru
authors.initials: T
authors.lastname: Kiyono
authors.affiliation: Department of Clinical Laboratories, Aichi Cancer Center Hospital, Nagoya, Aichi, Japan.
authors.forename: Yasutaka
authors.initials: Y
authors.lastname: Okada
authors.affiliation: Department of Haematology and Cell Therapy, Aichi Cancer Center Hospital, Nagoya, Aichi, Japan.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Kinoshita
authors.affiliation: Division of Molecular Medicine, Aichi Cancer Center Research Institute, Nagoya, Aichi, Japan.
authors.forename: Masao
authors.initials: M
authors.lastname: Seto
mesh_terms: D000328:Adult; D000368:Aged; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D020566:Dendritic Cells, Follicular / Q000502:physiology*; D005260:Female; D005838:Genotype; D006801:Humans; D007376:Interleukin-2 / Q000502:physiology*; D015847:Interleukin-4 / Q000502:physiology*; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000473:pathology*; D008297:Male; D053278:OX40 Ligand / Q000502:physiology*; D059016:Tumor Microenvironment
chemical_list: C508598:IL4 protein, human; D007376:Interleukin-2; D053278:OX40 Ligand; C089251:TNFSF4 protein, human; D015847:Interleukin-4
keywords: Adult T-cell leukemia/lymphoma; Cell line; Microenvironment