title: Changes of skin collagen orientation associated with chronological aging as probed by polarized-FTIR micro-imaging.
abstract: During chronological skin aging, alterations in dermal structural proteins cause morphological modifications. Modifications are probably due to collagen fiber (type I collagen) rearrangement and reorientation with aging that have not been researched until now. FTIR microspectroscopy appears as an interesting method to study protein structure under normal and pathological conditions. Associated with a polarizer, this vibrational technique permits us to probe collagen orientation within skin tissue sections, by computing the ratio of integrated intensities of amide I and amide II bands. In this study, we used the polarized-FTIR imaging to evaluate molecular modifications of dermal collagen during chronological aging. The data processing of polarized infrared data revealed that type I collagen fibers become parallel to the skin surface in aged skin dermis. Our approach could find innovative applications in dermatology as well as in cosmetics.
authors.affiliation: MéDIAN Biophotonique et Technologies pour la Santé, CNRS FRE 3481 MEDyC, SFR Cap-Santé, UFR Pharmacie, Université de Reims Champagne-Ardenne, 51 rue Cognacq-Jay, 51096 Reims Cedex, France. olivier.piot@univ-reims.fr.
authors.forename: The Thuong
authors.initials: TT
authors.lastname: Nguyen
authors.forename: Christophe
authors.initials: C
authors.lastname: Eklouh-Molinier
authors.forename: David
authors.initials: D
authors.lastname: Sebiskveradze
authors.forename: Jezabel
authors.initials: J
authors.lastname: Feru
authors.forename: Christine
authors.initials: C
authors.lastname: Terryn
authors.forename: Michel
authors.initials: M
authors.lastname: Manfait
authors.forename: Sylvie
authors.initials: S
authors.lastname: Brassart-Pasco
authors.forename: Olivier
authors.initials: O
authors.lastname: Piot
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000378:metabolism*; D000818:Animals; D016000:Cluster Analysis; D003094:Collagen / Q000378:metabolism*; D006801:Humans; D008875:Middle Aged; D051381:Rats; D012867:Skin / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
chemical_list: D003094:Collagen