title: Primary culture of trigeminal satellite glial cells: a cell-based platform to study morphology and function of peripheral glia.
abstract: Primary cell culture provides an experimental platform in which morphology, physiology, and cell-cell communication pathways can be studied under a well-controlled environment. Primary cell cultures of peripheral and central glia offer unique possibilities to clarify responses and pathways to different stimuli. Peripheral glia, satellite glial cells (SGCs), which surround neuronal cell bodies within sensory ganglia, have recently been known as key players in inflammation and neuronal sensitization. The objectives of this study were 1) to establish a cell-based platform of cultured trigeminal SGCs to study glial marker expression and functions under control conditions; 2) to validate the cell-based platform by prostaglandin E2 (PGE2) release response following administration of Cisplatin; and 3) to investigate inhibition of PGE2 release by glial modulators, Ibudilast and SKF86002. Primary cell cultures of SGCs from rat trigeminal ganglia were established following enzymatically and mechanically dissociation of the ganglia. Cultures were characterized in vitro for up to 21 days post isolation for morphological and immunocytochemical characteristics. PGE2 release, determined by ELISA, was used as a pro-inflammatory marker to characterize SGCs response to chemotherapeutic agent, Cisplatin, known to contribute in chemotherapy-induced peripheral neuropathy. Our results indicate that 1) isolated SGCs maintained their characteristics in vitro for up to 21 days; 2) Cisplatin enhanced PGE2 release from the SGCs, which was attenuated by Ibudilast and SKF86002. These findings confirm the utility and validity of the cultured trigeminal SGCs platform for glial activation and modulation; and suggest further investigation on Ibudilast and SKF86002 in prevention of chemotherapy-induced pain.
authors.affiliation: Center for Sensory-Motor Interaction, Department of Health Science and Technology, Faculty of Medicine, Aalborg University Fredrik Bajers Vej 7D3, DK-9220 Aalborg East, Denmark.
authors.forename: Jeppe N
authors.initials: JN
authors.lastname: Poulsen
authors.affiliation: Laboratory for Cancer Biology, Biomedicine,Department of Health Science and Technology, Faculty of Medicine, Aalborg University Fredrik Bajers Vej 3B, DK-9220 Aalborg East, Denmark.
authors.forename: Frederik
authors.initials: F
authors.lastname: Larsen
authors.affiliation: Laboratory for Cancer Biology, Biomedicine,Department of Health Science and Technology, Faculty of Medicine, Aalborg University Fredrik Bajers Vej 3B, DK-9220 Aalborg East, Denmark.
authors.forename: Meg
authors.initials: M
authors.lastname: Duroux
authors.affiliation: Center for Sensory-Motor Interaction, Department of Health Science and Technology, Faculty of Medicine, Aalborg University Fredrik Bajers Vej 7D3, DK-9220 Aalborg East, Denmark ; Laboratory for Cancer Biology, Biomedicine,Department of Health Science and Technology, Faculty of Medicine, Aalborg University Fredrik Bajers Vej 3B, DK-9220 Aalborg East, Denmark.
authors.forename: Parisa
authors.initials: P
authors.lastname: Gazerani
keywords: Cisplatin; Ibudilast; SKF86002; Satellite glial cells; primary culture; trigeminal ganglia