title: A display of pH-sensitive fusogenic GALA peptide facilitates endosomal escape from a Bio-nanocapsule via an endocytic uptake pathway.
abstract: BACKGROUND
  An affibody-displaying bio-nanocapsule (ZHER2-BNC) with a hepatocyte specificity derived from hepatitis B virus (HBV) was converted into an affibody, ZHER2, that recognizes HER2 receptors. This affibody was previously reported to be the result of the endocytosis-dependent specific uptake of proteins and siRNA into target cancer cells. To assist the endosomal escape of inclusions, a helper lipid with pH-sensitive fusogenic ability (1,2-dioleoyl-sn-glycero-3-phos phoethanolamine; DOPE) was conjugated with a ZHER2-BNC.
  
  
  RESULTS
  In this study, we displayed a pH-sensitive fusogenic GALA peptide on the surface of a particle in order to confer the ability of endosomal escape to a ZHER2-BNC. A GALA-displaying ZHER2-BNC purified from yeast uneventfully formed a particle structure. Furthermore, endosomal escape of the particle was facilitated after endocytic uptake and release of the inclusions to the cytoplasm without the cell toxicity.
  
  
  CONCLUSIONS
  The genetic fusion of a GALA peptide to the virus-like particle confers the ability of endosomal escape.
authors.forename: Yuya
authors.initials: Y
authors.lastname: Nishimura
authors.forename: Koichi
authors.initials: K
authors.lastname: Takeda
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Ezawa
authors.forename: Jun
authors.initials: J
authors.lastname: Ishii
authors.forename: Chiaki
authors.initials: C
authors.lastname: Ogino
authors.affiliation: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan. akondo@kobe-u.ac.jp.
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kondo
mesh_terms: D000595:Amino Acid Sequence; D002460:Cell Line; D004705:Endocytosis; D006514:Hepatitis B Surface Antigens / Q000032:analysis / Q000378:metabolism* / Q000648:ultrastructure; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D053769:Nanocapsules / Q000032:analysis* / Q000648:ultrastructure; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000008:administration & dosage* / Q000378:metabolism
chemical_list: D006514:Hepatitis B Surface Antigens; D053769:Nanocapsules; D010455:Peptides; D011993:Recombinant Fusion Proteins; C515865:Z(HER2.4)2 affibody; C066951:GALA peptide