title: Lens concentration of ofloxacin and lomefloxacin in an experimental endophthalmitis model.
abstract: Abstract.
  
  
  BACKGROUND
  Bacterial endophthalmitis is a serious complication of ocular surgery and penetrating trauma. The primary causative organisms are strains of Staphylococcus aureus and Staphylococcus epidermidis. Fluoroquinolones are widely used to treat endophthalmitis. There are a few studies on the penetration of fluoroquinolones into the lens in inflamed eyes. A literature search did not identify any data regarding penetration of topical ofloxacin into the lens in normal and inflamed eyes.
  
  
  OBJECTIVE
  The aim of this study was to determine the penetration of topical ofloxacin and lomefloxacin into the lens in a rabbit endophthalmitis model.
  
  
  METHODS
  New Zealand white rabbits were randomly divided into 2 groups. The left eyes were infected with an intravitreal inoculation of S aureus. The right eyes were used as a noninoculated control. Groups 1 and 2 received topical ofloxacin and lomefloxacin treatment, respectively, 24 hours after the inoculation. Two drops of the study drugs were instilled in the eyes every 30 minutes for 3 hours and then every 60 minutes for 3 hours. Lens samples were obtained 30 minutes after the last ofloxacin or lomefloxacin drops were administered. High-performance liquid chromatography was used to determine the fluoroquinolone concentration.
  
  
  RESULTS
  Ten rabbits were equally divided into the 2 treatment groups. There was no significant difference in mean (SD) lens concentrations between the control and inoculated eyes in either treatment group-ofloxacin (0.26 [0.32] μg/mL vs 0.11 [0.05] μg/mL, respectively) and lomefloxacin (0.50 [0.87] μg/mL vs 0.12 [0.08] μg/mL, respectively).
  
  
  CONCLUSIONS
  The results of this small experimental study found that topical ofloxacin and lomefloxacin can accumulate in the crystalline lens after installation. Inflammation did not affect the penetration of ofloxacin or lomefloxacin into the lens.
authors.affiliation: Ankara Training and Research Hospital, Department of Ophthalmology, Ankara, Turkey.
authors.forename: Ramazan
authors.initials: R
authors.lastname: Yağci
authors.affiliation: Ankara Training and Research Hospital, Department of Ophthalmology, Ankara, Turkey.
authors.forename: Yusuf
authors.initials: Y
authors.lastname: Oflu
authors.affiliation: Hacettepe University, School of Medicine, Department of Microbiology and Clinical Microbiology, Ankara, Turkey.
authors.forename: Server
authors.initials: S
authors.lastname: Yağci
authors.affiliation: Hacettepe University, School of Medicine, Department of Pharmacology, Ankara, Turkey.
authors.forename: Aysun
authors.initials: A
authors.lastname: Dinçel
authors.affiliation: Ankara Training and Research Hospital, Department of Microbiology, Ankara, Turkey.
authors.forename: Esma
authors.initials: E
authors.lastname: Kaya
authors.affiliation: Ankara Training and Research Hospital, Department of Ophthalmology, Ankara, Turkey.
authors.forename: Sunay
authors.initials: S
authors.lastname: Duman
authors.affiliation: Hacettepe University, School of Medicine, Department of Pharmacology, Ankara, Turkey.
authors.forename: Atilla
authors.initials: A
authors.lastname: Bozkurt
keywords: animal study; experimental endophthalmitis; fluoroquinolone; lens; lomefloxacin; ofloxacin; rabbit