title: Detection of enterovirus genome sequence from diarrheal feces of goat.
abstract: Goat diarrheal feces were subjected to metagenome analysis by the next-generation sequencing. Nucleotide sequences with homology to enteroviruses were obtained. Primers for RT-PCR were designed based on the nucleotide sequence of these sequences at the 5'-untranslated region, and we determined 563 bp nucleotide sequences that showed homology to bovine-like and ovine enteroviruses (77-87 %). We named the virus detected in this study goat enterovirus G1 (GEV-G1). In the phylogenetic analysis, GEV-G1 belonged to a cluster containing ovine enteroviruses. To our knowledge, this is the first report on nucleotide sequences of an enterovirus infecting Japanese goats.
authors.affiliation: Research and Education Center for Prevention of Global Infectious Diseases of Animal, Tokyo University of Agriculture and Technology, Saiwai, Fuchu, Tokyo, 183-8509, Japan.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Omatsu
authors.forename: Shinobu
authors.initials: S
authors.lastname: Tsuchiaka
authors.forename: Teppei
authors.initials: T
authors.lastname: Hirata
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Shiroma
authors.forename: Sachiko
authors.initials: S
authors.lastname: Okazaki
authors.forename: Yukie
authors.initials: Y
authors.lastname: Katayama
authors.forename: Mami
authors.initials: M
authors.lastname: Oba
authors.forename: Naomi
authors.initials: N
authors.lastname: Nishiura
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Sassa
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Furuya
authors.forename: Makoto
authors.initials: M
authors.lastname: Nagai
authors.forename: Hideharu
authors.initials: H
authors.lastname: Ochiai
authors.forename: Shirou
authors.initials: S
authors.lastname: Tamaki
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Mizutani
mesh_terms: D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003967:Diarrhea / Q000662:veterinary* / Q000821:virology; D004770:Enterovirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D005243:Feces / Q000821:virology*; D016679:Genome, Viral; D015511:Goat Diseases / Q000821:virology*; D006041:Goats; D008969:Molecular Sequence Data; D010802:Phylogeny; D012756:Sheep