title: Intestinal parasites and genotyping of Giardia duodenalis in children: first report of genotype B in isolates from human clinical samples in Mexico.
abstract: Giardia duodenalis is one of the most prevalent enteroparasites in children. This parasite produces several clinical manifestations. The aim of this study was to determine the prevalence of genotypes of G. duodenalis causing infection in a region of southeastern Mexico. G. duodenalis cysts were isolated (33/429) from stool samples of children and molecular genotyping was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis, targeting the triosephosphate isomerase ( tpi ) and glutamate dehydrogenase ( gdh ) genes. The tpi gene was amplified in all of the cyst samples, either for assemblage A (27 samples) or assemblage B (6 samples). RFLP analysis classified the 27 tpi -A amplicons in assemblage A, subgenotype I. Samples classified as assemblage B were further analysed using PCR-RFLP of the gdh gene and identified as assemblage B, subgenotype III. To our knowledge, this is the first report of assemblage B of G. duodenalis in human clinical samples from Mexico.
authors.affiliation: Laboratorio de Análisis Clínicos de Servicio a la Comunidad, Universidad Autónoma de Yucatán, Mérida, Yucatán, México.
authors.forename: Julio César
authors.initials: JC
authors.lastname: Torres-Romero
authors.affiliation: Laboratorio de Análisis Clínicos de Servicio a la Comunidad, Universidad Autónoma de Yucatán, Mérida, Yucatán, México.
authors.forename: Antonio de Jesus
authors.initials: Ade J
authors.lastname: Euan-Canto
authors.affiliation: Laboratorio de Análisis Clínicos de Servicio a la Comunidad, Universidad Autónoma de Yucatán, Mérida, Yucatán, México.
authors.forename: Namibya
authors.initials: N
authors.lastname: Benito-González
authors.affiliation: Laboratorio de Farmacología, Facultad de Química, Universidad Autónoma de Yucatán, Mérida, Yucatán, México.
authors.forename: Nayely
authors.initials: N
authors.lastname: Padilla-Montaño
authors.affiliation: Laboratorio de Análisis Clínicos de Servicio a la Comunidad, Universidad Autónoma de Yucatán, Mérida, Yucatán, México.
authors.forename: Claribel
authors.initials: C
authors.lastname: Huchin-Chan
authors.affiliation: Laboratorio de Análisis Clínicos de Servicio a la Comunidad, Universidad Autónoma de Yucatán, Mérida, Yucatán, México.
authors.forename: Julio
authors.initials: J
authors.lastname: Lara-Riegos
authors.affiliation: Unidad de Investigación Médica Yucatán, Unidad Médica de Alta Especialidad, Instituto Mexicano del Seguro Social, Mérida, Yucatán, México.
authors.forename: Roberto
authors.initials: R
authors.lastname: Cedillo-Rivera
mesh_terms: D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D016054:DNA, Protozoan / Q000032:analysis; D005243:Feces / Q000469:parasitology; D005260:Female; D005838:Genotype; D016829:Giardia lamblia / Q000235:genetics* / Q000302:isolation & purification; D005873:Giardiasis / Q000469:parasitology*; D006801:Humans; D007223:Infant; D008297:Male; D008800:Mexico; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA
chemical_list: D016054:DNA, Protozoan