title: Use and application of hydrophobic interaction chromatography for protein purification.
abstract: The objective of this section is to provide the reader with guidelines and background on the use and experimental application of Hydrophobic Interaction chromatography (HIC) for the purification of proteins. The section will give step by step instructions on how to use HIC in the laboratory to purify proteins. General guidelines and relevant background information is also provided.
authors.affiliation: Biogen Idec Corporation, 14 Cambridge Center, Cambridge, MA, USA. Electronic address: justin.mccue@biogenidec.com.
authors.forename: Justin T
authors.initials: JT
authors.lastname: McCue
mesh_terms: D002845:Chromatography / Q000295:instrumentation / Q000379:methods*; D057927:Hydrophobic and Hydrophilic Interactions; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification*
chemical_list: D011506:Proteins
keywords: Adsorbent regeneration and sanitization; Chaotropes; Column equilibration and loading; Hofmeister series; Hydrophobic interaction chromatography (HIC); Hydrophobic proteins; Lyotropes; Protein purification; Technology in HIC adsorbents