title: Potent antioxidative and UVB protective effect of water extract of Eclipta prostrata L.
abstract: Oxidative stress, including Ultraviolet (UV) irradiation-induced skin damage, is involved in numerous diseases. This study demonstrates that water extract of Eclipta prostrata L. (WEP) has a potent effect in scavenging 1,1-diphenyl-2-picrylhydrazyl (DPPH), superoxide radicals, and chelating ferrous ion, exhibiting IC50 values of 0.23 mg/mL, 0.48 mg/mL, and 1.25 mg/mL, respectively. The WEP total phenol content was 176.45 mg gallic acid equivalents (GAE)/g sample. Chlorogenic acid, a component of the plant's active ingredients, was determined by HPLC and antioxidative assay. However, no caffeic acid, stigmasterol, or wedelolactone was present in WEP. WEP absorbs both UVA and UVB irradiation, and furthermore, the extract shows a dose-dependent response in the protection of HaCaT human keratinocytes and mouse fibroblasts 3T3 cells against UVB-induced cytotoxicity, which may result from a synergistic effect between chlorogenic acid and other active components present in WEP.
authors.affiliation: Department of Applied Cosmetology, Hungkuang University, Taichung 43302, Taiwan.
authors.forename: Chin-Feng
authors.identifier: 0000-0003-1681-8651
authors.initials: CF
authors.lastname: Chan
authors.affiliation: Department of Applied Cosmetology, Hungkuang University, Taichung 43302, Taiwan.
authors.forename: Wen-Ying
authors.initials: WY
authors.lastname: Huang
authors.affiliation: Department of Applied Cosmetology, Hungkuang University, Taichung 43302, Taiwan.
authors.forename: Hong-Yi
authors.identifier: 0000-0001-7149-6021
authors.initials: HY
authors.lastname: Guo
authors.affiliation: Department of Applied Cosmetology, Hungkuang University, Taichung 43302, Taiwan.
authors.forename: Bo Rong
authors.initials: BR
authors.lastname: Wang
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D036601:Eclipta / Q000737:chemistry*; D006801:Humans; D020128:Inhibitory Concentration 50; D010936:Plant Extracts / Q000494:pharmacology*; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D013056:Spectrophotometry, Ultraviolet; D014466:Ultraviolet Rays; D014867:Water
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D011837:Radiation-Protective Agents; D014867:Water