title: Patch-clamp recording of voltage-sensitive Ca2+ channels.
abstract: In this article, we focus on a refinement of the traditional voltage-clamp methods that are used to measure current from whole cells, or relatively large areas of membrane, called the patch-clamp technique. Although this technique has extended the application of voltage-clamp methods to the recording of ionic currents flowing through single channels, in its whole-cell configuration it has become the most widely used method for recording ionic currents. We give particular attention to the study of voltage-gated (CaV) Ca(2+) channels using the patch-clamp technique and discuss some aspects of the molecular physiology of these proteins.
authors.affiliation: Department of Cell Biology, Center for Research and Advanced Studies of the National Polytechnic Institute (Cinvestav-IPN), Mexico City, Mexico;
authors.forename: María A
authors.initials: MA
authors.lastname: Gandini
authors.forename: Alejandro
authors.initials: A
authors.lastname: Sandoval
authors.forename: Ricardo
authors.initials: R
authors.lastname: Felix
mesh_terms: D000818:Animals; D015220:Calcium Channels / Q000737:chemistry / Q000378:metabolism*; D020013:Calcium Signaling; D002462:Cell Membrane / Q000737:chemistry; D018408:Patch-Clamp Techniques / Q000379:methods*
chemical_list: D015220:Calcium Channels