title: A reverse transcription loop-mediated isothermal amplification assay to rapidly diagnose foot-and-mouth disease virus C.
abstract: A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed to rapidly detect foot-and-mouth disease virus serotype C (FMDV C). By testing 10-fold serial dilutions of FMDV C samples, sensitivity of the FMDV C RT-LAMP was found to be 10 times higher than that of conventional reverse transcription- PCR (RT-PCR). No cross-reactivity with A, Asia 1, or O FMDV or swine vesicular disease virus (SVDV) indicated that FMDV C RT-LAMP may be an exciting novel method for detecting FMDV C.
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, OIE/National Foot and Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
authors.forename: Yao-Zhong
authors.initials: YZ
authors.lastname: Ding
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Zhou
authors.forename: Li-Na
authors.initials: LN
authors.lastname: Ma
authors.forename: Yan-Ni
authors.initials: YN
authors.lastname: Qi
authors.forename: Gang
authors.initials: G
authors.lastname: Wei
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.forename: Yong-Guang
authors.initials: YG
authors.lastname: Zhang
mesh_terms: D000818:Animals; D005536:Foot-and-Mouth Disease / Q000175:diagnosis*; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics; D021141:Nucleic Acid Amplification Techniques / Q000379:methods* / Q000662:veterinary; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D048348:Reverse Transcription / Q000235:genetics; D012680:Sensitivity and Specificity
keywords: foot-and-mouth disease; foot-and-mouth disease virus serotype C; reverse transcription loop-mediated isothermal amplification; reverse transcription-PCR; sensitivity