title: Designed synthesis of titania nanoparticles coated hierarchially ordered macro/mesoporous silica for selective enrichment of phosphopeptides.
abstract: Metal oxide affinity chromatography (MOAC) is a powerful technique in phosphoproteome research. However, the achievement of highly specific enrichment and sensitive detection of phosphopeptide by MOAC remains a big challenge since the lack of high specificity and large binding capacity of conventional MOAC materials. In this work, a new MOAC material, TiO2-coated hierarchically ordered macro/mesoporous silica (denoted as HOMMS@TiO2) composites, was prepared via a facile process. The HOMMS@TiO2 composites were demonstrated to have low limit of detection (8 fmol) and great specificity with a very rapid enrichment speed (within 1 min). These experimental results have demonstrated that the HOMMS@TiO2 exhibit great potential in phosphoproteome research.
authors.affiliation: Department of Chemistry, Fudan University , Shanghai 200433, China.
authors.forename: Yinghua
authors.initials: Y
authors.lastname: Yan
authors.forename: Xiangmin
authors.initials: X
authors.lastname: Zhang
authors.forename: Chunhui
authors.initials: C
authors.lastname: Deng
mesh_terms: D000327:Adsorption; D002846:Chromatography, Affinity / Q000295:instrumentation / Q000379:methods*; D013058:Mass Spectrometry; D053758:Nanoparticles / Q000737:chemistry*; D010748:Phosphopeptides / Q000737:chemistry / Q000302:isolation & purification*; D012822:Silicon Dioxide / Q000737:chemistry*; D014025:Titanium / Q000737:chemistry*
chemical_list: D010748:Phosphopeptides; C009495:titanium dioxide; D012822:Silicon Dioxide; D014025:Titanium