title: Applications of recombinant Pichia pastoris in the healthcare industry.
abstract: Since the 1970s, the establishment and development of the biotech industry has improved exponentially, allowing the commercial production of biopharmaceutical proteins. Nowadays, new recombinant protein production is considered a multibillion-dollar market, in which about 25% of commercial pharmaceuticals are biopharmaceuticals. But to achieve a competitive production process is not an easy task. Any production process has to be highly productive, efficient and economic. Despite that the perfect host is still not discovered, several research groups have chosen Pichia pastoris as expression system for the production of their protein because of its many features. The attempt of this review is to embrace several research lines that have adopted Pichia pastoris as their expression system to produce a protein on an industrial scale in the health care industry.
authors.affiliation: Departamento de Ingeniería Química, Facultad de Ingeniería, Ciencias y Administración, Universidad de La Frontera, Temuco, Chile.
authors.forename: Daniel
authors.initials: D
authors.lastname: Weinacker
authors.affiliation: Departamento de Producción Agropecuaria, Facultad de Ciencias Agropecuarias y Forestales, Universidad de La Frontera, Temuco, Chile.
authors.forename: Claudia
authors.initials: C
authors.lastname: Rabert
authors.affiliation: Departamento de Ingeniería Química, Facultad de Ingeniería, Ciencias y Administración, Universidad de La Frontera, Temuco, Chile.
authors.forename: Andrea B
authors.initials: AB
authors.lastname: Zepeda
authors.affiliation: Departamento de Ingeniería Química, Facultad de Ingeniería, Ciencias y Administración, Universidad de La Frontera, Temuco, Chile.
authors.forename: Carolina A
authors.initials: CA
authors.lastname: Figueroa
authors.affiliation: Departamento de Tecnologia Bioquímico-Farmacêutica, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, São Paulo, SP, Brazil.
authors.forename: Adalberto
authors.initials: A
authors.lastname: Pessoa
authors.affiliation: Departamento de Ingeniería Química, Facultad de Ingeniería, Ciencias y Administración, Universidad de La Frontera, Temuco, Chile.
authors.forename: Jorge G
authors.initials: JG
authors.lastname: Farías
mesh_terms: D001709:Biotechnology / Q000379:methods*; D019981:Health Care Sector; D006801:Humans; D007218:Industrial Microbiology / Q000379:methods*; D030781:Organisms, Genetically Modified; D010843:Pichia / Q000235:genetics* / Q000378:metabolism*; D013678:Technology, Pharmaceutical / Q000379:methods*
keywords: heterologous expression system; production; recombinant protein; scale up; yeast