title: Lentiviral-mediated expression of SATB2 promotes osteogenic differentiation of bone marrow stromal cells in vitro and in vivo.
abstract: Special AT-rich sequence-binding protein 2 (SATB2 ) acts as a potent transcription factor to promote osteoblast differentiation and bone regeneration. In this study, we first used lentiviral-mediated gene transfer of Satb2 into mouse bone marrow stromal cells (BMSCs) and investigated the capacity of SATB2 overexpression to promote osteogenic differentiation in vitro and in vivo. We found that LV-Satb2 -transduced BMSCs produced SATB2 protein and underwent rapid and marked osteogenic differentiation, as demonstrated by increased expression of osteoblastic genes, including runt-related transcription factor 2 (Runx2), transcription factor Sp7 (Sp7), activating transcription factor 4 (Atf4), and bone sialoprotein (Bsp), and increased alkaline phosphatase activity and Alizarin Red S staining. To analyze the induction of bone formation in vivo, LV-Satb2-transduced BMSCs were implanted into the hindlimbs of syngeneic mice, with β-tricalcium phosphate as the scaffolding material. Four weeks after implantation, transduction with LV-Satb2 had greatly enhanced the formation of new bone. These data demonstrated the capacity of lentiviral-mediated SATB2 to promote the osteogenic differentiation of BMSCs in vitro and to enhance bone formation through a tissue-engineering technique that may be useful in bone-regenerative medicine.
authors.affiliation: Department of Stomatology, Zhongshan Hospital, Fudan University, Shanghai, China.
authors.forename: Yiming
authors.initials: Y
authors.lastname: Gong
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Qian
authors.forename: Fei
authors.initials: F
authors.lastname: Yang
authors.forename: Huijun
authors.initials: H
authors.lastname: Wang
authors.forename: Youcheng
authors.initials: Y
authors.lastname: Yu
mesh_terms: D051701:Activating Transcription Factor 4 / Q000032:analysis; D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D001861:Bone Regeneration / Q000502:physiology; D002130:Calcium Phosphates / Q000737:chemistry; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000235:genetics; D050659:Core Binding Factor Alpha 1 Subunit / Q000032:analysis; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000235:genetics*; D058574:Integrin-Binding Sialoprotein / Q000032:analysis; D016086:Lentivirus / Q000235:genetics*; D008297:Male; D036961:Matrix Attachment Region Binding Proteins / Q000235:genetics*; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D010006:Osteoblasts / Q000502:physiology; D010012:Osteogenesis / Q000235:genetics*; D000076182:Sp7 Transcription Factor; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry; D014157:Transcription Factors / Q000032:analysis / Q000235:genetics*; D014185:Transplantation, Isogeneic; D016335:Zinc Fingers / Q000235:genetics
chemical_list: C081452:Atf4 protein, mouse; D002130:Calcium Phosphates; D050659:Core Binding Factor Alpha 1 Subunit; C546872:Ibsp protein, mouse; D058574:Integrin-Binding Sialoprotein; D036961:Matrix Attachment Region Binding Proteins; C493716:Runx2 protein, mouse; C501473:SATB2 protein, mouse; D000076182:Sp7 Transcription Factor; C479466:Sp7 protein, mouse; D014157:Transcription Factors; C485817:beta-tricalcium phosphate; D051701:Activating Transcription Factor 4; D000469:Alkaline Phosphatase
keywords: SATB2; bone marrow stromal cell; lentivirus; osteogenic differentiation; β-tricalcium phosphate