title: Effect of Brahman genetic influence on collagen enzymatic crosslinking gene expression and meat tenderness.
abstract: The objective of the study was to examine the effect of Brahman genetics on collagen enzymatic crosslinking gene expression and meat tenderness. Steers were randomly selected to represent a high percentage Brahman genetics (n = 13), Half-Blood genetics (n = 13), Brangus genetics (n = 13), and a high percentage Angus genetics (n = 13). Muscle samples from the Longissimus lumborum muscle were collected at weaning and harvest and reverse transcription quantitative PCR (qPCR) analysis was conducted to measure the mRNA expression of lysyl oxidase (LOX), bone morphogenetic protein 1 (BMP1), and cystatin C (CYS). Steaks from subject animals were collected at harvest, aged for 14 d and subjected to collagen analysis, Warner-Bratzler Shear Force (WBS) and trained sensory panel analysis (tenderness, juiciness, and connective tissue). Data indicated that Half-Blood and Brahman steers had greater (P<0.05) WBS values and tended to receive decreased (P < 0.06) panel tenderness scores than Angus and Brangus steers. Panelists tended to detect more connective tissue in Brahman and Half-Blood steaks when compared to Angus and Brangus steaks (P < 0.07). Crosslinking gene expression data revealed that at weaning Half-Blood steers had more (P < 0.05) mRNA expression of CYS and LOX than Angus and Brangus steers. At weaning and harvest, all genetic groups had similar mRNA expression of BMP1 (P > 0.10). At harvest, Brangus and Angus steers had greater LOX mRNA expression than Brahman cattle (P < 0.05). Pearson's correlation coefficients indicated that only weaning CYS mRNA expression was correlated to WBS, panel tenderness and connective tissue scores (P < 0.05). Expression of LOX was only correlated to these measures at harvest, and BMP1 was correlated to these traits at both time periods (P < 0.05). These results indicate that collagen crosslinking enzyme activity, as indicated by mRNA levels, early in an animal's life may account for some of the variation seen in steak tenderness due to Brahman genetic influence.
authors.affiliation: a Department of Animal Sciences and Industry , Kansas State University , Manhattan , Kansas , USA.
authors.forename: J M
authors.initials: JM
authors.lastname: Gonzalez
authors.forename: D D
authors.initials: DD
authors.lastname: Johnson
authors.forename: M A
authors.initials: MA
authors.lastname: Elzo
authors.forename: M C
authors.initials: MC
authors.lastname: White
authors.forename: A M
authors.initials: AM
authors.lastname: Stelzleni
authors.forename: S E
authors.initials: SE
authors.lastname: Johnson
mesh_terms: D000818:Animals; D055395:Bone Morphogenetic Protein 1 / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D002417:Cattle / Q000235:genetics*; D003094:Collagen / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D055316:Cystatin C / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D008297:Male; D008460:Meat / Q000032:analysis*; D016133:Polymerase Chain Reaction; D008249:Protein-Lysine 6-Oxidase / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D014886:Weaning
chemical_list: D055316:Cystatin C; D012333:RNA, Messenger; D003094:Collagen; D008249:Protein-Lysine 6-Oxidase; D055395:Bone Morphogenetic Protein 1