title: Isolation and immunosuppressive effects of 6″-O-acetylginsenoside Rb1 extracted from North American ginseng.
abstract: Extraction of medicinally active components from natural health products has become an emerging source for drug discovery. Of particular interest for this work was the finding and testing of a new ginsenoside from North American ginseng (Panax quinquefolius). In the present study, a large amount of 6″-O-acetylginsenoside Rb1, compound 7, was found using ultrasonic extraction of North American ginseng with DMSO aqueous solution. This new ginsenoside was well identified with MS, FTIR, and 1D (1H and 13C) and 2D (gCOSY, gHSQC, and gHMBC) NMR. Subsequent bioassay experiments confirmed that compound 7 demonstrated an additional immunosuppressive activity towards inhibiting the production of nitric oxide and tumor necrosis factor alpha in lipopolysaccharide-induced macrophage cells in a dose-dependent manner using murine macrophages. This new ginsenoside is encouraging for the further exploration and development of novel drugs.
authors.affiliation: Department of Chemical and Biochemical Engineering, Western University, London, Canada.
authors.forename: Raziye
authors.initials: R
authors.lastname: Samimi
authors.affiliation: Department of Chemical and Biochemical Engineering, Western University, London, Canada.
authors.forename: William Z
authors.initials: WZ
authors.lastname: Xu
authors.affiliation: Department of Physiology and Pharmacology, Western University, London, Canada.
authors.forename: Edmund M K
authors.initials: EM
authors.lastname: Lui
authors.affiliation: Department of Chemical and Biochemical Engineering, Western University, London, Canada.
authors.forename: Paul A
authors.initials: PA
authors.lastname: Charpentier
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use; D004305:Dose-Response Relationship, Drug; D036145:Ginsenosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D007166:Immunosuppressive Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D008070:Lipopolysaccharides; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D017942:Nitric Acid / Q000378:metabolism; D005894:Panax / Q000737:chemistry*; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: C000593456:6''-O-acetylginsenoside Rb1; D000893:Anti-Inflammatory Agents; D036145:Ginsenosides; D007166:Immunosuppressive Agents; D008070:Lipopolysaccharides; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D017942:Nitric Acid