title: Pathogenicity and molecular characteristics of infectious bronchitis virus (IBV) strains isolated from broilers showing diarrhoea and respiratory disease.
abstract: Abstract 1. The possibility that infectious bronchitis virus (IBV) variants isolated from broilers with enteric and respiratory problems have a different tropism and pathological outcome from those IBV strains causing classical respiratory disease was investigated. 2. IBV variants were isolated from broiler flocks with enteric and respiratory problems in two regions of Brazil. The USP-10 isolate, of enteric origin, was inoculated via the oral oroculonasal routes into IBV-antibody-free broilers and specific pathogen-free (SPF) chickens to determine tissue tropism and pathogenicity and compared with an IBV variant (USP-50) isolated from chickens showing signs of respiratory disease only. 3. Both USP-10 and USP-50 strains caused similar pathological patterns by either route of inoculation. Both variants were detected in respiratory and non-respiratory tissues, including the kidney, intestine and testis. 4. Broilers were more susceptible to infection than SPF chickens, and seroconversion was detected in all of the chicks.
authors.affiliation: a Department of Pathology , School of Veterinary Medicine - University of São Paulo , Av. Prof. Dr. Orlando M. Paiva, 87, CEP 05508-900 , São Paulo , SP , Brazil.
authors.forename: J L
authors.initials: JL
authors.lastname: Chacón
authors.forename: M S
authors.initials: MS
authors.lastname: Assayag
authors.forename: L
authors.initials: L
authors.lastname: Revolledo
authors.forename: C S
authors.initials: CS
authors.lastname: Astolfi-Ferreira
authors.forename: M P
authors.initials: MP
authors.lastname: Vejarano
authors.forename: R C
authors.initials: RC
authors.lastname: Jones
authors.forename: A J
authors.initials: AJ
authors.lastname: Piantino Ferreira
mesh_terms: D000818:Animals; D002645:Chickens / Q000276:immunology / Q000821:virology*; D018352:Coronavirus Infections / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D004196:Disease Outbreaks / Q000662:veterinary; D001351:Infectious bronchitis virus / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity