title: Tannin analysis of chestnut bark samples (Castanea sativa Mill.) by HPLC-DAD-MS.
abstract: In the present investigation, an HPLC-DAD/ESI-MS method for the complete analysis of tannins and other phenolic compounds of different commercial chestnut bark samples was developed. A total of seven compounds (vescalin, castalin, gallic acid, vescalagin, 1-O-galloyl castalagin, castalagin and ellagic acid) were separated and quantified, being 1-O-galloyl castalagin tentatively identified and found for the first time in chestnut bark samples. Thus, this method provided information regarding the composition and quality of chestnut bark samples, which is required since these samples are commercialised due to their biochemical properties as ingredients of food supplements.
authors.affiliation: Department of Food Science, University of Bologna, Piazza Goidanich 60, I-47521 Cesena, FC, Italy. Electronic address: patrizia.comandini2@unibo.it.
authors.forename: Patrizia
authors.initials: P
authors.lastname: Comandini
authors.affiliation: Department of Analytical Chemistry, University of Valencia, Doctor Moliner 50, E-46100 Burjassot, Valencia, Spain.
authors.forename: María Jesús
authors.initials: MJ
authors.lastname: Lerma-García
authors.affiliation: Department of Analytical Chemistry, University of Valencia, Doctor Moliner 50, E-46100 Burjassot, Valencia, Spain.
authors.forename: Ernesto Francisco
authors.initials: EF
authors.lastname: Simó-Alfonso
authors.affiliation: Department of Food Science, University of Bologna, Piazza Goidanich 60, I-47521 Cesena, FC, Italy.
authors.forename: Tullia Gallina
authors.initials: TG
authors.lastname: Toschi
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D029962:Fagaceae / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D009754:Nuts / Q000737:chemistry*; D010636:Phenols / Q000032:analysis / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D013634:Tannins / Q000737:chemistry*
chemical_list: D010636:Phenols; D010936:Plant Extracts; D013634:Tannins
keywords: Chestnut bark; Ellagitannins; HPLC-DAD–MS