title: Mycobacterium tuberculosis belonging to family LAM and sublineage RD(Rio): common strains in Southern Brazil for over 10 years.
abstract: A sublineage of Mycobacterium tuberculosis called RD(Rio) was described in 2007. Although only recently described, this strain may have been present previously in the population, and its identification in clinical isolates will elucidate bacterial transmission dynamics and host-pathogen interactions. This study evaluated the clonal diversity of the RD(Rio) sublineage in clinical isolates from Rio Grande-RS obtained between 1998 and 2001. Among the 45 samples analyzed by the MIRU-VNTR method, there were six clusters with two samples each and 33 orphan strains with unique pattern. The strains were distributed across several different lineages including LAM (34.04%), × (14.89%), Haarlem (12.77%), UgandaI (10.64%), S (4.26%), NEW-1 (2.13%) and Cameroon (2.13%); 14.89% of the strains matched to multiple lineages. RD(Rio) strains were present in 28.9% of the samples and 81.25% of the identified strains belonged to the LAM family. The high clonal diversity observed in this study is a constant feature in this region. The RD(Rio) sublineage has been in Rio Grande-RS since 1998. The continued monitoring of RD(Rio) in clinical isolates will enhance the understanding of its epidemiological significance.
authors.affiliation: Universidade Federal do Rio Grande, Rio Grande, RS, Brazil.
authors.forename: Renata Oliveira
authors.initials: RO
authors.lastname: Soares
authors.affiliation: Universidade Federal do Rio Grande, Rio Grande, RS, Brazil.
authors.forename: Maíra Bidart
authors.initials: MB
authors.lastname: de Macedo
authors.affiliation: Universidade Federal do Rio Grande, Rio Grande, RS, Brazil.
authors.forename: Andrea
authors.initials: A
authors.lastname: von Groll
authors.affiliation: Universidade Federal do Rio Grande, Rio Grande, RS, Brazil.
authors.forename: Pedro Eduardo Almeida
authors.initials: PE
authors.lastname: da Silva
mesh_terms: D001938:Brazil / Q000453:epidemiology; D016000:Cluster Analysis; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D018598:Minisatellite Repeats; D017720:Molecular Epidemiology; D058889:Molecular Typing; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D014376:Tuberculosis / Q000453:epidemiology* / Q000382:microbiology*
keywords: MIRU-VNTR; Mycobacterium tuberculosis; RDRio