title: Metabolomic analysis of diet-induced type 2 diabetes using UPLC/MS integrated with pattern recognition approach.
abstract: Metabolomics represents an emerging discipline concerned with comprehensive assessment of small molecule endogenous metabolites in biological systems and provides a powerful approach insight into the mechanisms of diseases. Type 2 diabetes (T2D), called the burden of the 21st century, is growing with an epidemic rate. However, its precise molecular mechanism has not been comprehensively explored. In this study, we applied urinary metabolomics based on the UPLC/MS integrated with pattern recognition approaches to discover differentiating metabolites, to characterize and explore metabolic pathway disruption in an experimental model for high-fat-diet induced T2D. Six differentiating urinary metabolites were found in the negative mode, and two (2-(4-hydroxy-3-methoxy-phenyl) acetaldehyde sulfate, 2-phenylethanol glucuronide) of which were identified involving the key metabolic pathways linked to pentose and glucuronate interconversions, starch, sucrose metabolism and tyrosine metabolism. Our study provides new insight into pathophysiologic mechanisms and may enhance the understanding of T2D pathogenesis.
authors.affiliation: Department of Pharmaceutical Analysis, Key Lab of Metabolomics and Chinmedomics, National TCM Key Laboratory of Serum Pharmacochemistry, Heilongjiang University of Chinese Medicine, Harbin, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Sun
authors.affiliation: Department of Pharmaceutical Analysis, Key Lab of Metabolomics and Chinmedomics, National TCM Key Laboratory of Serum Pharmacochemistry, Heilongjiang University of Chinese Medicine, Harbin, China.
authors.forename: Shuxiang
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Pharmaceutical Analysis, Key Lab of Metabolomics and Chinmedomics, National TCM Key Laboratory of Serum Pharmacochemistry, Heilongjiang University of Chinese Medicine, Harbin, China.
authors.forename: Aihua
authors.initials: A
authors.lastname: Zhang
authors.affiliation: Department of Pharmaceutical Analysis, Key Lab of Metabolomics and Chinmedomics, National TCM Key Laboratory of Serum Pharmacochemistry, Heilongjiang University of Chinese Medicine, Harbin, China.
authors.forename: Guangli
authors.initials: G
authors.lastname: Yan
authors.affiliation: Department of Pharmaceutical Analysis, Key Lab of Metabolomics and Chinmedomics, National TCM Key Laboratory of Serum Pharmacochemistry, Heilongjiang University of Chinese Medicine, Harbin, China.
authors.forename: Xiuhong
authors.initials: X
authors.lastname: Wu
authors.affiliation: Department of Pharmaceutical Analysis, Key Lab of Metabolomics and Chinmedomics, National TCM Key Laboratory of Serum Pharmacochemistry, Heilongjiang University of Chinese Medicine, Harbin, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Han
authors.affiliation: Department of Pharmaceutical Analysis, Key Lab of Metabolomics and Chinmedomics, National TCM Key Laboratory of Serum Pharmacochemistry, Heilongjiang University of Chinese Medicine, Harbin, China.
authors.forename: Xijun
authors.initials: X
authors.lastname: Wang
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000652:urine; D003924:Diabetes Mellitus, Type 2 / Q000209:etiology / Q000652:urine*; D059305:Diet, High-Fat / Q000009:adverse effects*; D008297:Male; D055442:Metabolome; D010363:Pattern Recognition, Automated; D017208:Rats, Wistar; D053719:Tandem Mass Spectrometry
chemical_list: D015415:Biomarkers