title: Pharmacological evaluation of the efficacy of Dysoxylum binectariferum stem bark and its active constituent rohitukine in regulation of dyslipidemia in rats.
abstract: The antidyslipidemic effect of the ethanolic extract of Dysoxylum binectariferum stem bark and its major active constituent rohitukine was evaluated in a high fat diet (HFD)-fed dyslipidemic rat model. Chronic feeding of ethanolic extract (200 mg/kg) in HFD-fed rats showed significant lipid lowering activity. The bioassay guided fractionation of ethanolic extract resulted in the identification of known alkaloid rohitukine as major active constituent. Rohitukine (50 mg/kg) significantly decreased the plasma levels of total cholesterol (24 %), phospholipids (25 %), triglycerides (27 %), very low density lipoprotein (27 %) and low density lipoprotein (32 %) accompanied with an increase in high density lipoprotein (21 %). The present study demonstrated that ethanolic extract of Dysoxylum binectariferum stem bark and its major constituent rohitukine both have antidyslipidemic as well as antioxidant potentials. The antidyslipidemic activity of rohitukine can be correlated to its effect on enzymes involved in lipid metabolism.
authors.affiliation: Medicinal and Process Chemistry Division, CSIR-Central Drug Research Institute, Lucknow, 226001, India.
authors.forename: Sunil K
authors.initials: SK
authors.lastname: Mishra
authors.affiliation: Department of Pesticide Toxicology, CSIR-Indian Institute of Toxicology Research, Lucknow, 226001, India.
authors.forename: Shashi
authors.initials: S
authors.lastname: Tiwari
authors.affiliation: Medicinal and Process Chemistry Division, CSIR-Central Drug Research Institute, Lucknow, 226001, India.
authors.forename: Shishir
authors.initials: S
authors.lastname: Shrivastava
authors.affiliation: Department of Biochemistry, CSIR-Central Drug Research Institute, Lucknow, 226001, India.
authors.forename: Ravi
authors.initials: R
authors.lastname: Sonkar
authors.affiliation: Department of Pharmacology, CSIR-Central Drug Research Institute, Lucknow, 226001, India.
authors.forename: Vaibhav
authors.initials: V
authors.lastname: Mishra
authors.affiliation: Department of Chemistry, Govt. Science College Rewa, Rewa, 486001, India.
authors.forename: Sunil K
authors.initials: SK
authors.lastname: Nigam
authors.affiliation: Medicinal and Process Chemistry Division, CSIR-Central Drug Research Institute, Lucknow, 226001, India.
authors.forename: Anil K
authors.initials: AK
authors.lastname: Saxena
authors.affiliation: Department of Biochemistry, CSIR-Central Drug Research Institute, Lucknow, 226001, India.
authors.forename: Gitika
authors.initials: G
authors.lastname: Bhatia
authors.affiliation: Department of Biotechnology, Integral University, Lucknow, 226026, India. mir_51@yahoo.in.
authors.forename: Snober S
authors.initials: SS
authors.lastname: Mir
mesh_terms: D000818:Animals; D000975:Antioxidants; D002867:Chromones / Q000494:pharmacology / Q000627:therapeutic use*; D050171:Dyslipidemias / Q000188:drug therapy*; D050356:Lipid Metabolism / Q000187:drug effects*; D008297:Male; D010880:Piperidines / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats
chemical_list: C573233:5,7-dihydroxy-2-methyl-8-(4-(3-hydroxy-1-methyl)-piperidinyl)-4H-1-benzopyran-4-one; D000975:Antioxidants; D002867:Chromones; D010880:Piperidines
keywords: Antidyslipidemic; Antioxidant; Dyslipidemia; Dysoxylum binectariferum; HMG–CoA reductase; Lecithin-cholesterol acyl transferase activity