title: Investigation of characteristics of feather follicle stem cells and their regeneration potential.
abstract: Feather follicles have the extraordinary ability to regenerate and undergo molting cycles. Being tissue-specific stem cells, feather follicle stem cells (FFSCs) have a strong capacity for proliferation and are presumed to be progenitor cells for various epidermal organs. In order to characterize FFSCs and to understand how the feather epidermis and FFSCs produce such a reliable differentiation program resulting in the formation of complex feathers, We developed a culture scheme to select and expand FFSCs from chick feather follicles. FFSCs were examined with cell profiles, mutilpotential differentiation and immunocytochemical staining. FFSCs from a single clone were capable of self-renewal and proliferation. These cells expressed integrin β1, CD49c, cytokeratin 15 (K15), cytokeratin 19 (K19) and a neural-genic cell marker, nestin, but not a teminal differentiation-related keratinocyte marker, cytokeratin 10 (K10). FFSCs could trans-differentiate into adipocytes, neurocytes and keratinocytes. The formation of micro-feather like structures ex-vivo also revealed the potential of regeneration. These results demonstrate that FFSCs possess the properties of stem/progenitor cells and may therefore serve as a useful model for studying mechanisms of stem cell differentiation and their involvement in organ regeneration.
authors.affiliation: Institute of Cell Biology, College of Medicine, Zhejiang University , China ; Institute of Genetics and Cell Biology, College of Life Sciences, Zhejiang University , China.
authors.forename: Y L
authors.initials: YL
authors.lastname: Xu
authors.affiliation: Institute of Genetics and Cell Biology, College of Life Sciences, Zhejiang University , China.
authors.forename: J G
authors.initials: JG
authors.lastname: Sun
authors.affiliation: Institute of Genetics and Cell Biology, College of Life Sciences, Zhejiang University , China.
authors.forename: F B
authors.initials: FB
authors.lastname: Wu
authors.affiliation: Institute of Genetics and Cell Biology, College of Life Sciences, Zhejiang University , China.
authors.forename: Y M
authors.initials: YM
authors.lastname: Xi
keywords: Feather follicle stem cells; regeneration; trans-differentiation