title: In vitro selection of RNA aptamers directed against protein E: a Haemophilus influenzae adhesin.
abstract: Protein E (PE) of Haemophilus influenzae is a highly conserved ubiquitous surface protein involved in adhesion to and activation of epithelial cells. The host proteins-vitronectin, laminin, and plasminogen are major targets for PE-dependent interactions with the host. To identify novel inhibitory molecules of PE, we used an in vitro selection method based on systematic evolution of ligands by exponential enrichment known as SELEX in order to select 2'F-modified RNA aptamers that specifically bind to PE. Fourteen selection cycles were performed with decreasing concentrations of PE. Sequencing of clones from the 14th selection round revealed the presence of semiconserved sequence motifs in loop regions of the RNA aptamers. Among these, three aptamers showed the highest affinity to PE in electrophoretic mobility shift assays and in dot blots. These three aptamers also inhibited the interaction of PE with vitronectin as revealed by ELISA. Moreover, pre-treatment of H. influenzae with the aptamers significantly inhibited binding of vitronectin to the bacterial surface. Biacore experiments indicated that one of the aptamers had a higher binding affinity for PE as compared to the other aptamers. Our results show that it is possible to select RNA inhibitors against bacterial adhesins using SELEX in order to inhibit interactions with target proteins.
authors.affiliation: Department of Biochemistry and Structural Biology, CMPS, Lund University, Box 124, 221 00, Lund, Sweden, anders.barfod@biochemistry.lu.se.
authors.forename: Anders
authors.initials: A
authors.lastname: Barfod
authors.forename: Birendra
authors.initials: B
authors.lastname: Singh
authors.forename: Urban
authors.initials: U
authors.lastname: Johanson
authors.forename: Kristian
authors.initials: K
authors.lastname: Riesbeck
authors.forename: Per
authors.initials: P
authors.lastname: Kjellbom
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics* / Q000378:metabolism; D052157:Aptamers, Nucleotide / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D001709:Biotechnology; D004269:DNA, Bacterial / Q000235:genetics; D006193:Haemophilus influenzae / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity; D006801:Humans; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D011485:Protein Binding; D052156:SELEX Aptamer Technique; D020349:Surface Plasmon Resonance; D019096:Vitronectin / Q000378:metabolism
chemical_list: D018829:Adhesins, Bacterial; D052157:Aptamers, Nucleotide; D004269:DNA, Bacterial; D019096:Vitronectin