title: [Expression of plasma miR-106a in colorectal cancer and its clinical significance].
abstract: OBJECTIVE
  To detect plasma miR-106a level in patients with colorectal cancer (CRC) and analyze its correlation to the clinicopathological features and disease diagnosis.
  
  
  METHODS
  miRNA expression profiling was performed using miRNA microarray chip for 3 colorectal adenocarcinoma samples and matched normal tissues. Plasma samples was collected from 50 colorectal cancer patients for quantitative analysis of miR-106a using real-time RT-PCR using 47 plasma samples from healthy volunteer as the control. Forty plasma samples were collected from these patients 7 days after operation to examine the changes in miR-106a expression.
  
  
  RESULTS
  miR-106a was differentially expressed in colorectal adenocarcinoma compared to normal tissues. The plasma levels of miR-106a expression were significantly higher in the cancer patients than in the healthy control group (P=0.012). miR-106a expression significantly decreased after the operation compared with its preoperative level (P<0.01), and no correlation was found between preoperative plasma miR-106a and the clinicopathological features including lymph node metastasis and TNM stage (P>0.05). miR-106a showed a receiver operating characteristic (ROC) curve area of 66.1%, a sensitivity of 62.3%, and a specificity of 68.2% in discriminating colorectal cancer patients from the control subjects.
  
  
  CONCLUSIONS
  plasma miR-106a is up-regulated in CRC patients, suggesting its potential value for the diagnosis of CRC.
authors.affiliation: Department of General Surgery, First Hospital of Hebei Medical University, Shijiazhuang 050031, China. E-mail: lijing_4871@163.com.
authors.forename: Lijing
authors.initials: L
authors.lastname: Zhang
authors.forename: Limin
authors.initials: L
authors.lastname: Meng
authors.forename: Zhibin
authors.initials: Z
authors.lastname: Fan
authors.forename: Bo
authors.initials: B
authors.lastname: Liu
authors.forename: Yongbin
authors.initials: Y
authors.lastname: Pei
authors.forename: Zengren
authors.initials: Z
authors.lastname: Zhao
mesh_terms: D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D015179:Colorectal Neoplasms / Q000097:blood* / Q000175:diagnosis*; D005260:Female; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D012680:Sensitivity and Specificity; D015854:Up-Regulation
chemical_list: C542541:MIRN106 microRNA, human; D035683:MicroRNAs