title: Elucidating proline dynamics in spider dragline silk fibre using 2H-13C HETCOR MAS NMR.
abstract: (2)H-(13)C HETCOR MAS NMR is performed on (2)H/(13)C/(15)N-Pro enriched A. aurantia dragline silk. Proline dynamics are extracted from (2)H NMR line shapes and T1 in a site-specific manner to elucidate the backbone and side chain molecular dynamics for the MaSp2 GPGXX β-turn regions for spider dragline silk in the dry and wet, supercontracted states.
authors.affiliation: Department of Chemistry and Biochemistry, Magnetic Resonance Research Center, Arizona State University, Tempe, AZ 85287-1604, USA. greg.holland@asu.edu jyarger@gmail.com.
authors.forename: Xiangyan
authors.initials: X
authors.lastname: Shi
authors.forename: Jeffery L
authors.initials: JL
authors.lastname: Yarger
authors.forename: Gregory P
authors.initials: GP
authors.lastname: Holland
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002247:Carbon Isotopes / Q000737:chemistry; D066241:Carbon-13 Magnetic Resonance Spectroscopy / Q000379:methods*; D003903:Deuterium / Q000737:chemistry*; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011392:Proline / Q000032:analysis* / Q000235:genetics*; D017433:Protein Structure, Secondary; D047011:Silk; D013112:Spiders
chemical_list: D002247:Carbon Isotopes; D047011:Silk; D011392:Proline; D003903:Deuterium