title: Hyperketonemia during lipopolysaccharide-induced mastitis affects systemic and local intramammary metabolism in dairy cows.
abstract: Hyperketonemia interferes with the metabolic regulation in dairy cows. It is assumed that metabolic and endocrine changes during hyperketonemia also affect metabolic adaptations during inflammatory processes. We therefore studied systemic and local intramammary effects of elevated plasma β-hydroxybutyrate (BHBA) before and during the response to an intramammary lipopolysaccharide (LPS) challenge. Thirteen dairy cows received intravenously either a Na-DL-β-OH-butyrate infusion (n = 5) to achieve a constant plasma BHBA concentration (1.7 ± 0.1 mmol/L), with adjustments of the infusion rates made based on immediate measurements of plasma BHBA every 15 min, or an infusion with a 0.9% NaCl solution (control; n = 8) for 56 h. Infusions started at 0900 h on d 1 and continued until 1700 h 2 d later. Two udder quarters were challenged with 200 μg of Escherichia coli LPS and 2 udder quarters were treated with 0.9% saline solution as control quarters at 48 h after the start of infusion. Blood samples were taken at 1 wk and 2h before the start of infusions as reference samples and hourly during the infusion. Mammary gland biopsies were taken 1 wk before, and 48 and 56 h (8h after LPS challenge) after the start of infusions. The mRNA abundance of key factors related to BHBA and fatty acid metabolism, and glucose transporters was determined in mammary tissue biopsies. Blood samples were analyzed for plasma glucose, BHBA, nonesterified fatty acid, urea, insulin, glucagon, and cortisol concentrations. Differences were not different for effects of BHBA infusion on the mRNA abundance of any of the measured target genes in the mammary gland before LPS challenge. Intramammary LPS challenge increased plasma glucose, cortisol, glucagon, and insulin concentrations in both groups but increases in plasma glucose and glucagon concentration were less pronounced in the Na-DL-β-OH-butyrate infusion group than in controls. In response to LPS challenge, plasma BHBA concentration decreased in controls and decreased also slightly in the BHBA-infused animals because the BHBA concentration could not be fully maintained despite a rapid increase in BHBA infusion rate. The change in mRNA abundance of citrate synthase in LPS quarters was significant between the 2 treatment groups. The results indicate that elevated circulating BHBA concentration inhibits gluconeogenesis before and during immune response to LPS challenge, likely because BHBA can replace glucose as an energy source.
authors.affiliation: Veterinary Physiology, Vetsuisse Faculty, University of Bern, Bremgartenstrasse 109a, 3001 Bern, Switzerland; Department of Animal Science, Agriculture Faculty, Yasouj University, Student Street, 75918-74831, Yasouj, I. R. Iran; Graduate School for Cellular and Biomedical Sciences, University of Bern, Freiestrasse 1, 3012 Bern, Switzerland.
authors.forename: M
authors.initials: M
authors.lastname: Zarrin
authors.affiliation: Veterinary Physiology, Vetsuisse Faculty, University of Bern, Bremgartenstrasse 109a, 3001 Bern, Switzerland.
authors.forename: O
authors.initials: O
authors.lastname: Wellnitz
authors.affiliation: Veterinary Physiology, Vetsuisse Faculty, University of Bern, Bremgartenstrasse 109a, 3001 Bern, Switzerland.
authors.forename: H A
authors.initials: HA
authors.lastname: van Dorland
authors.affiliation: Veterinary Physiology, Vetsuisse Faculty, University of Bern, Bremgartenstrasse 109a, 3001 Bern, Switzerland.
authors.forename: J J
authors.initials: JJ
authors.lastname: Gross
authors.affiliation: Veterinary Physiology, Vetsuisse Faculty, University of Bern, Bremgartenstrasse 109a, 3001 Bern, Switzerland. Electronic address: rupert.bruckmaier@vetsuisse.unibe.ch.
authors.forename: R M
authors.initials: RM
authors.lastname: Bruckmaier
mesh_terms: D020155:3-Hydroxybutyric Acid / Q000097:blood; D000103:Acetyl-CoA Carboxylase / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D002417:Cattle; D002950:Citrate (si)-Synthase / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D064429:Fatty Acid Synthases / Q000235:genetics / Q000378:metabolism; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005260:Female; D005934:Glucagon / Q000097:blood; D005943:Gluconeogenesis / Q000187:drug effects; D051246:Glucose Transport Proteins, Facilitative / Q000235:genetics / Q000378:metabolism; D006854:Hydrocortisone / Q000097:blood; D006863:Hydrogen-Ion Concentration; D006884:Hydroxybutyrate Dehydrogenase / Q000235:genetics / Q000378:metabolism; D007328:Insulin / Q000097:blood; D007662:Ketosis / Q000097:blood* / Q000662:veterinary*; D008070:Lipopolysaccharides / Q000009:adverse effects*; D008321:Mammary Glands, Animal / Q000378:metabolism* / Q000503:physiopathology; D008414:Mastitis, Bovine / Q000097:blood* / Q000139:chemically induced; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D001786:Blood Glucose; D005230:Fatty Acids, Nonesterified; D051246:Glucose Transport Proteins, Facilitative; D007328:Insulin; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D005934:Glucagon; D006884:Hydroxybutyrate Dehydrogenase; D064429:Fatty Acid Synthases; D002950:Citrate (si)-Synthase; D000103:Acetyl-CoA Carboxylase; D020155:3-Hydroxybutyric Acid; D006854:Hydrocortisone
keywords: lipopolysaccharide challenge; mRNA abundance; metabolism; β-hydroxybutyrate