title: Green Synthesis of Robust, Biocompatible Silver Nanoparticles Using Garlic Extract.
abstract: This paper details a facile approach for the synthesis of stable and monodisperse silver nanoparticles performed at ambient/low temperature whereAllium sativum(garlic) extract functions as the silver salt reducing agent during nanoparticle synthesis as well as the post-synthesis stabilizing ligands. Varying the synthesis conditions provides control of particle size, size-distribution, and kinetics of particle formation. Infrared spectroscopy, energy dispersive x-ray chemical analysis, and high performance liquid chromatography indicated that the carbohydrates present in the garlic extract are the most likely nanoparticle stabilizing chemistry. The synthesized silver nanoparticles also demonstrate potential for biomeical applications, owing to the 1) enhanced stability in biological media, 2) resistance to oxidation by the addition of H2O2, 3) ease and scalability of synthesis, and 4) lack of harsh chemicals required for synthesis. Cytotoxicity assays indicated no decrease in cellular proliferation for vascular smooth muscle cells and 3T3 fibroblasts at a concentration of 25 μg/ml, confirming that garlic extract prepared silver nanoparticles are ideal candidates for future experimentation and implementation into biomedical applications.
authors.affiliation: Department of Chemical and Biomolecular Engineering, Clemson University, Clemson, SC 29634.
authors.forename: Gregory
authors.initials: G
authors.lastname: Von White
authors.affiliation: Department of Chemical and Biomolecular Engineering, Clemson University, Clemson, SC 29634.
authors.forename: Petra
authors.initials: P
authors.lastname: Kerscher
authors.affiliation: Department of Chemical and Biomolecular Engineering, Clemson University, Clemson, SC 29634.
authors.forename: Ryan M
authors.initials: RM
authors.lastname: Brown
authors.affiliation: Department of Chemical and Biomolecular Engineering, Clemson University, Clemson, SC 29634.
authors.forename: Jacob D
authors.initials: JD
authors.lastname: Morella
authors.affiliation: Department of Bioengineering, Clemson University, Clemson, SC 29634.
authors.forename: William
authors.initials: W
authors.lastname: McAllister
authors.affiliation: Department of Bioengineering, Clemson University, Clemson, SC 29634.
authors.forename: Delphine
authors.initials: D
authors.lastname: Dean
authors.affiliation: Department of Chemical and Biomolecular Engineering, Clemson University, Clemson, SC 29634.
authors.forename: Christopher L
authors.initials: CL
authors.lastname: Kitchens
keywords: 3T3 fibroblasts; cytotoxicity; garlic; green nanotechnology; silver nanoparticles