title: Multicore magnetic nanoparticles (MMNPs) doped with Cs and FITC for the determination of biomarker in serum using ICP-MS.
abstract: Multicore magnetic nanoparticles (MMNPs) doped with Cs and FITC (Cs/FITC-doped MMNPs) were synthesized for the extraction and determination of biomarkers using inductively coupled plasma-mass spectrometry (ICP-MS). For demonstration, the MMNPs were used for magnetic separation to extract CA19-9 in serum nonspecifically, and the doped Cs was used as an internal standard for the ratiometric measurement of the tagged particle. This ratiometric method compensated for the particle loss in a magnetic separation and suppressed the signal fluctuation which increased the calibration linearity significantly. The obtained detection limit was 0.02 units/mL of CA19-9, which is more than 300 times lower than that reported by the ICP-MS with element tagging and about 500-fold improved compared to ELISA.
authors.affiliation: Department of Chemistry, NSBI, Dankook University , 126 Jukjeon-dong, Suji-gu, Yongin-si, Gyeonggi-do 448-701, Korea.
authors.forename: Jungaa
authors.initials: J
authors.lastname: Ko
authors.forename: Heung Bin
authors.initials: HB
authors.lastname: Lim
mesh_terms: D015415:Biomarkers / Q000097:blood*; D002586:Cesium / Q000737:chemistry*; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry*; D008280:Magnetics; D013058:Mass Spectrometry / Q000379:methods*; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles
chemical_list: D015415:Biomarkers; D002586:Cesium; D016650:Fluorescein-5-isothiocyanate