title: Plant genome engineering in full bloom.
abstract: The recent development of tools for precise editing of user-specified sequences is rapidly changing the landscape for plant genetics and biotechnology. It is now possible to target mutations and regulatory proteins to specific sites in a genome using zinc-finger nucleases (ZFNs), transcription activator-like endonucleases (TALENs), or the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) system. Here we provide an update of recent developments in CRISPR/Cas9 technology and highlight online resources that will help biologists adopt new genome-editing tools.
authors.affiliation: Department of Botany and Plant Sciences, University of California, Riverside, CA 92521, USA; Center for Plant Cell Biology, University of California, Riverside, CA 92521, USA; Institute for Integrative Genome Biology, University of California, Riverside, CA 92521, USA.
authors.forename: Jorge
authors.initials: J
authors.lastname: Lozano-Juste
authors.affiliation: Department of Botany and Plant Sciences, University of California, Riverside, CA 92521, USA; Center for Plant Cell Biology, University of California, Riverside, CA 92521, USA; Institute for Integrative Genome Biology, University of California, Riverside, CA 92521, USA. Electronic address: sean.cutler@ucr.edu.
authors.forename: Sean R
authors.initials: SR
authors.lastname: Cutler
mesh_terms: D001709:Biotechnology; D064113:CRISPR-Cas Systems / Q000235:genetics; D064112:Clustered Regularly Interspaced Short Palindromic Repeats / Q000235:genetics*; D004720:Endonucleases / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D018390:Gene Targeting / Q000379:methods*; D005818:Genetic Engineering / Q000379:methods*; D018745:Genome, Plant / Q000235:genetics*; D009154:Mutation; D010944:Plants / Q000235:genetics*; D049490:Systems Biology; D016335:Zinc Fingers
chemical_list: D004720:Endonucleases
keywords: CRISPR; Cas9; TALENs; ZFNs; genome engineering; plant