title: A novel role of globular adiponectin in treatment with HFD/STZ induced T2DM combined with NAFLD rats.
abstract: OBJECTIVE
  To evaluate the effects of globular adiponectin (gAd) on treatment of type 2 diabetic rats combined with NAFLD.
  
  
  METHODS
  Twenty-one male wistar rats were fed with normal diet (7 rats) or high fat diet (HFD) (14 rats) for 4 weeks, and then HFD-fed rats were injected with streptozotocin (STZ) to induce type 2 diabetes mellitus (T2DM). Half of T2DM rats were randomly injected with gAd intraperitoneally for 7 days. The expressions of adiponectin receptors (adipoR1/R2) in liver and skeletal muscle tissues were detected through western blotting or RT-qPCR, respectively.
  
  
  RESULTS
  Globular adiponectin alleviated the hepatic steatosis and increased insulin secretion. In liver, both the protein and mRNA expressions of adipoR2 in T2DM group decreased (P < 0.05, resp.) in contrast to NC group and increased (P < 0.05 and P < 0.001, resp.) after gAd treatment. But the protein and mRNA expressions of adipoR1 increased (P < 0.05, resp.) in T2DM group and no change was found in the gAd-treated group. In skeletal muscle, the protein and mRNA expressions of adipoR1 and adipoR2 were upregulated in T2DM group and were downregulated after gAd treatment.
  
  
  CONCLUSIONS
  Globular adiponectin could ameliorate the hepatic steatosis and vary the expressions of adiponectin receptors in liver and skeletal muscle by stimulating insulin secretion.
authors.affiliation: Department of Endocrinology, Zhongshan Hospital Xiamen University, 209 Hubin South Road, Xiamen, Fujian 361004, China ; Department of Endocrinology, Xiamen Zhongshan Teaching Hospital of Fujian Medical University, Xiamen, Fujian 361004, China.
authors.forename: Hong
authors.identifier: 0000-0003-4415-694X
authors.initials: H
authors.lastname: Ma
authors.affiliation: Department of Endocrinology, Zhongshan Hospital Xiamen University, 209 Hubin South Road, Xiamen, Fujian 361004, China ; Department of Endocrinology, Xiamen Zhongshan Teaching Hospital of Fujian Medical University, Xiamen, Fujian 361004, China.
authors.forename: Guo-Ping
authors.initials: GP
authors.lastname: You
authors.affiliation: Department of Endocrinology, Zhongshan Hospital Xiamen University, 209 Hubin South Road, Xiamen, Fujian 361004, China ; Department of Endocrinology, Xiamen Zhongshan Teaching Hospital of Fujian Medical University, Xiamen, Fujian 361004, China.
authors.forename: Xuan-Pu
authors.initials: XP
authors.lastname: Zhang
authors.affiliation: Department of Endocrinology, Zhongshan Hospital Xiamen University, 209 Hubin South Road, Xiamen, Fujian 361004, China.
authors.forename: Xiang-Jiu
authors.initials: XJ
authors.lastname: Yang
authors.affiliation: Department of Pathology, Zhongshan Hospital Xiamen University, Xiamen, Fujian 361004, China.
authors.forename: Hua-Dong
authors.initials: HD
authors.lastname: Lu
authors.affiliation: Department of Endocrinology, Zhongshan Hospital Xiamen University, 209 Hubin South Road, Xiamen, Fujian 361004, China.
authors.forename: Yan-Ling
authors.initials: YL
authors.lastname: Huang
authors.affiliation: Department of Endocrinology, Zhongshan Hospital Xiamen University, 209 Hubin South Road, Xiamen, Fujian 361004, China.
authors.forename: Wen-Qiang
authors.initials: WQ
authors.lastname: Zhang
mesh_terms: D052242:Adiponectin / Q000008:administration & dosage*; D000818:Animals; D003924:Diabetes Mellitus, Type 2 / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism*; D059305:Diet, High-Fat; D004305:Dose-Response Relationship, Drug; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D018482:Muscle, Skeletal / Q000187:drug effects / Q000378:metabolism; D065626:Non-alcoholic Fatty Liver Disease / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D054419:Receptors, Adiponectin / Q000378:metabolism*; D013311:Streptozocin; D016896:Treatment Outcome
chemical_list: D052242:Adiponectin; D054419:Receptors, Adiponectin; D013311:Streptozocin