title: Recent advances in enrichment and separation strategies for mass spectrometry-based phosphoproteomics.
abstract: Due to the significance of protein phosphorylation in various biological processes and signaling events, new analytical techniques for enhanced phosphoproteomics have been rapidly introduced in the recent years. The combinatorial use of the phospho-specific enrichment techniques and prefractionation methods prior to MS analysis enable comprehensive profiling of the phosphoproteome and facilitate deciphering the critical roles that phosphorylation plays in signaling pathways in various biological systems. This review places special emphasis on the recent five-year (2009-2013) advances for enrichment and separation techniques that have been utilized for phosphopeptides prior to MS analysis.
authors.affiliation: Department of Chemistry, University of Wisconsin, Madison, WI, USA.
authors.forename: Chenxi
authors.initials: C
authors.lastname: Yang
authors.forename: Xuefei
authors.initials: X
authors.lastname: Zhong
authors.forename: Lingjun
authors.initials: L
authors.lastname: Li
mesh_terms: D000818:Animals; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D010748:Phosphopeptides / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D010750:Phosphoproteins / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D040901:Proteomics / Q000379:methods*
chemical_list: D010748:Phosphopeptides; D010750:Phosphoproteins
keywords: Enrichment; Mass spectrometry; Phosphopeptides; Phosphoproteomics/Separation