title: [Simultaneous determination of five Amadori compounds in tobacco by high performance liquid chromatography-mass spectrometry].
abstract: A method of high performance liquid chromatography-triple quadrupole mass spectrometry (HPLC-QqQ MS) in multiple reaction monitoring (MRM) mode for simultaneous determination of five Amadori compounds in tobacco has been developed. The separation was performed on an XBridge Amide column (250 mm x 4.6 mm, 3.5 microm) by gradient elution using methanol and water as mobile phases. The linearity of this method was good with the correlation coefficients (r) of 0.9895-0.9989. The limits of detection (S/N = 3) were from 3.51 microg/L to 14.86 microg/L, and the limits of quantitation (S/N = 10) were from 10.18 microg/L to 44.58 microg/L. The recoveries varied from 92.6% to 123.6% with the relative standard deviations (RSDs) less than 9.4%. The established method is rapid, accurate, robust and convenient, and it could be applied to the measurement of the five Amadori compounds in tobacco and cigarettes.
authors.forename: Hongrui
authors.initials: H
authors.lastname: Wang
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Xie
authors.forename: Qionglin
authors.initials: Q
authors.lastname: Liang
authors.forename: Yiming
authors.initials: Y
authors.lastname: Wang
authors.forename: Ping
authors.initials: P
authors.lastname: Hu
authors.forename: Guoan
authors.initials: G
authors.lastname: Luo
mesh_terms: D002851:Chromatography, High Pressure Liquid; D053719:Tandem Mass Spectrometry; D014026:Nicotiana / Q000737:chemistry*