title: Circulating CD1c(+) DCs are superior at activating Th2 responses upon Phl p stimulation compared with basophils and pDCs.
abstract: The contributing role of circulating human dendritic cell (DC) populations and basophils in the presentation and augmentation of Th2 responses remains to be determined. The present study aimed at elucidating the functional role of CD1c(+) myeloid DCs (mDCs), CD123(+) plasmacytoid DCs (pDCs), monocyte-derived DCs and basophils in allergen presentation and Th2 activation. By coculturing Phleum pratense (Phl p)-pulsed CD1c(+) mDCs, CD123(+) pDCs, monocyte-derived DCs and basophils with autologous CD4(+) effector memory T cells, we assessed T-cell proliferation as well as the frequency of interleukin-4- and interferon-γ-producing T cells. Interestingly, a Th2-stimulating ability was observed for Phl p-challenged CD1c(+) mDCs and monocyte-derived DCs, while CD123(+) pDCs and basophils did not affect the Th-balance. In addition, both Phl p-pulsed CD1c(+) mDCs and monocyte-derived DCs stimulated increased T-cell proliferation compared to basophils and CD123(+) pDCs. Together, these results point to a prominent role for circulating CD1c(+) mDCs in allergen presentation and augmentation of Th2 responses, making them promising therapeutic targets for Type I hypersensitivity reactions.
authors.affiliation: Department of Immunotechnology, Lund University, Lund, Sweden.
authors.forename: Frida
authors.initials: F
authors.lastname: Rydnert
authors.affiliation: Department of Immunotechnology, Lund University, Lund, Sweden.
authors.forename: Kristina
authors.initials: K
authors.lastname: Lundberg
authors.affiliation: Department of Otorhinolaryngology, Head & Neck Surgery, Skåne University Hospital, Lund, Sweden.
authors.forename: Lennart
authors.initials: L
authors.lastname: Greiff
authors.affiliation: Department of Immunotechnology, Lund University, Lund, Sweden.
authors.forename: Malin
authors.identifier: 0000000191361087
authors.initials: M
authors.lastname: Lindstedt
mesh_terms: D000485:Allergens / Q000276:immunology*; D017951:Antigen Presentation; D018949:Antigens, CD1 / Q000276:immunology*; D001491:Basophils / Q000276:immunology* / Q000473:pathology; D003713:Dendritic Cells / Q000276:immunology* / Q000473:pathology; D005260:Female; D006023:Glycoproteins / Q000276:immunology*; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000276:immunology / Q000473:pathology / Q000628:therapy; D053650:Interleukin-3 Receptor alpha Subunit / Q000276:immunology; D008297:Male; D009000:Monocytes / Q000276:immunology* / Q000473:pathology; D010940:Plant Proteins / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology* / Q000473:pathology
chemical_list: D000485:Allergens; D018949:Antigens, CD1; C437944:CD1C protein, human; D006023:Glycoproteins; C115022:IL3RA protein, human; D053650:Interleukin-3 Receptor alpha Subunit; D010940:Plant Proteins; C090396:PHLPI protein, Phleum pratense