title: Nanostructured electrochemical biosensor for th0065 detection of the weak binding between the dengue virus and the CLEC5A receptor.
abstract: In this paper, we develop an effective method for detecting weak molecular bonding between the dengue virus (DV) and its receptor C-type lectin domain family 5, member A (CLEC5A). The CLEC5A-DV interaction is critical for DV-induced hemorrhagic fever and shock syndrome, so the sensing of CLEC5A-DV binding is crucial to realize a thorough study of the pathogenesis of dengue fever. Through a highly sensitive nanostructured sensing electrode of gold nanoparticles (GNPs) uniformly deposited on a nanohemisphere array, a label-free detection of the ultra weak binding between CLEC5A and the DV can be performed with electrochemical impedance spectroscopy (EIS). Experimental results demonstrate that the proposed approach is a highly promising method for investigating weak molecular interactions such as the ligand-receptor interaction of dengue fever, enterovirus (EV), or the interaction between cancer surface glycoproteins and their receptors.
  
  
  BACKGROUND
  Authors of this study investigated the ultra-weak binding between dengue virus and its CLEC5A receptor via electrochemical impedance spectroscopy and gold NP sensing electrode. Similar methods may be applicable in other infections and in cancer models as well.
authors.affiliation: Ph.D. Program in Tissue Engineering and Regenerative Medicine, National Chung-Hsing University, Taichung, Taiwan. Electronic address: tencred@hotmail.com.
authors.forename: Yen-Ting
authors.initials: YT
authors.lastname: Tung
authors.affiliation: Genomics Research Center, Academia Sinica, Taipei, Taiwan. Electronic address: wmf680102@gmail.com.
authors.forename: Ming-Fang
authors.initials: MF
authors.lastname: Wu
authors.affiliation: Ph.D. Program in Tissue Engineering and Regenerative Medicine, National Chung-Hsing University, Taichung, Taiwan; Graduate Institute of Biomedical Engineering, National Chung-Hsing University, Taichung, Taiwan. Electronic address: gjwang@dragon.nchu.edu.tw.
authors.forename: Gou-Jen
authors.initials: GJ
authors.lastname: Wang
authors.affiliation: Institute of Clinical Medicine, National Yang-Ming University, Taipei, Taiwan; Genomics Research Center, Academia Sinica, Taipei, Taiwan; Immunology Center, Taipei Veterans General Hospital, Taipei, Taiwan; Institute for Cancer Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University, Taipei, Taiwan. Electronic address: slhsieh@gate.sinica.edu.tw.
authors.forename: Shie-Liang
authors.initials: SL
authors.lastname: Hsieh
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D003715:Dengue / Q000378:metabolism*; D003716:Dengue Virus / Q000378:metabolism*; D058266:Dielectric Spectroscopy / Q000295:instrumentation*; D004566:Electrodes; D004867:Equipment Design; D006046:Gold / Q000737:chemistry; D006801:Humans; D037181:Lectins, C-Type / Q000378:metabolism*; D049329:Nanostructures / Q000737:chemistry / Q000648:ultrastructure; D011485:Protein Binding; D011956:Receptors, Cell Surface / Q000378:metabolism*
chemical_list: C509771:CLEC5A protein, human; D037181:Lectins, C-Type; D011956:Receptors, Cell Surface; D006046:Gold
keywords: Anodic aluminum oxide; CLEC5A; Dengue virus; Electrochemical impedance spectroscopy; Nanostructured biosensor