title: Waste degradation and gas production with enzymatic enhancement in anaerobic and aerobic landfill bioreactors.
abstract: The presence of lignin is the limiting factor at later stages of biodegradation of municipal solid waste under aerobic or anaerobic conditions. Supplying enzymes into the system could facilitate lignin degradation, thereby aiding anaerobic and aerobic waste degradation processes. A comprehensive set of laboratory experiments were conducted under both anaerobic and aerobic conditions to evaluate the feasibility of using enzymes in accelerating lignin-rich waste degradation. After 30 days of anaerobic operation, MnP and LiP enzyme treated reactors produced 36 and 23 times higher cumulative methane (CH4), respectively, compared to that of the control reactor devoid of enzyme treatments. The carbon dioxide (CO2) yield of MnP enhanced aerobic reactor showed more than two-fold increase.
authors.affiliation: Centre for Environmental Engineering Research and Education (CEERE), Schulich School of Engineering, University of Calgary, Calgary, Alberta T2N 1N4, Canada. Electronic address: jhettiar@ucalgary.ca.
authors.forename: J P A
authors.initials: JP
authors.lastname: Hettiaratchi
authors.affiliation: Centre for Environmental Engineering Research and Education (CEERE), Schulich School of Engineering, University of Calgary, Calgary, Alberta T2N 1N4, Canada.
authors.forename: P A
authors.initials: PA
authors.lastname: Jayasinghe
authors.affiliation: Centre for Environmental Engineering Research and Education (CEERE), Schulich School of Engineering, University of Calgary, Calgary, Alberta T2N 1N4, Canada.
authors.forename: E M
authors.initials: EM
authors.lastname: Bartholameuz
authors.affiliation: Council of Scientific and Industrial Research-National Environmental Engineering Research Institute (CSIR-NEERI), Nehru Marg, Nagpur 440020, India.
authors.forename: S
authors.initials: S
authors.lastname: Kumar
mesh_terms: D000332:Aerobiosis; D000693:Anaerobiosis; D000704:Analysis of Variance; D001673:Biodegradation, Environmental; D019149:Bioreactors / Q000382:microbiology*; D002245:Carbon Dioxide / Q000378:metabolism; D005740:Gases / Q000378:metabolism*; D008697:Methane / Q000096:biosynthesis; D010544:Peroxidases / Q000378:metabolism*; D012037:Refuse Disposal; D064091:Waste Disposal Facilities / Q000295:instrumentation*
chemical_list: D005740:Gases; D002245:Carbon Dioxide; D010544:Peroxidases; C042858:lignin peroxidase; C051129:manganese peroxidase; D008697:Methane
keywords: Aerobic degradation; Anaerobic degradation; Bioreactor landfills; Enzymatic enhancement; Lignin degradation