title: Optimization of medium using response surface methodology for lipid production by Scenedesmus sp.
abstract: Lipid production is an important indicator for assessing microalgal species for biodiesel production. In this work, the effects of medium composition on lipid production by Scenedesmus sp. were investigated using the response surface methodology. The results of a Plackett-Burman design experiment revealed that NaHCO₃, NaH₂PO4·2H₂O and NaNO₃ were three factors significantly influencing lipid production, which were further optimized by a Box-Behnken design. The optimal medium was found to contain 3.07 g L⁻¹ NaHCO₃, 15.49 mg L⁻¹ NaH₂PO4·2H₂O and 803.21 mg L⁻¹ NaNO₃. Using the optimal conditions previously determined, the lipid production (304.02 mg·L⁻¹) increased 54.64% more than that using the initial medium, which agreed well with the predicted value 309.50 mg L⁻¹. Additionally, lipid analysis found that palmitic acid (C16:0) and oleic acid (C18:1) dominantly constituted the algal fatty acids (about 60% of the total fatty acids) and a much higher content of neutral lipid accounted for 82.32% of total lipids, which strongly proved that Scenedesmus sp. is a very promising feedstock for biodiesel production.
authors.affiliation: Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China. ycuyang@163.com.
authors.forename: Fangfang
authors.initials: F
authors.lastname: Yang
authors.affiliation: Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China. longlj@scsio.ac.cn.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Long
authors.affiliation: Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China. sunxm@scsio.ac.cn.
authors.forename: Xiumei
authors.initials: X
authors.lastname: Sun
authors.affiliation: Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China. hlwu@scsio.ac.cn.
authors.forename: Hualian
authors.initials: H
authors.lastname: Wu
authors.affiliation: Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China. taoli@scsio.ac.cn.
authors.forename: Tao
authors.initials: T
authors.lastname: Li
authors.affiliation: Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China. xwz@scsio.ac.cn.
authors.forename: Wenzhou
authors.initials: W
authors.lastname: Xiang
mesh_terms: D000465:Algorithms; D056804:Biofuels; D018533:Biomass; D003470:Culture Media; D005227:Fatty Acids / Q000737:chemistry / Q000302:isolation & purification; D005502:Food; D008055:Lipids / Q000096:biosynthesis*; D015233:Models, Statistical; D009566:Nitrates / Q000737:chemistry; D019301:Oleic Acid / Q000737:chemistry / Q000302:isolation & purification; D019308:Palmitic Acid / Q000737:chemistry / Q000302:isolation & purification; D010710:Phosphates; D044444:Scenedesmus / Q000378:metabolism*; D017693:Sodium Bicarbonate
chemical_list: D056804:Biofuels; D003470:Culture Media; D005227:Fatty Acids; D008055:Lipids; D009566:Nitrates; D010710:Phosphates; D019301:Oleic Acid; D019308:Palmitic Acid; C031618:sodium nitrate; D017693:Sodium Bicarbonate; C018279:sodium phosphate