title: Development of a capillary zone electrophoresis method for determination of mebendazole and levamisole hydrochloride in a combined tablet and a comparison with a LC method.
abstract: The separation and determination of mebendazole (MEB) and levamisole hydrochloride (LH) from a combination tablet by capillary zone electrophoresis (CZE) was developed and validated. The separation was performed in a 60 cm (50.5 cm to detection window) x 75 microm internal diameter fused silica capillary using a background electrolyte of NaH2PO4 (20 mM/L, pH 3.0 ) at 25 kV. Good separation was obtained in less than 8 min. The LOQ values for LH and MEB were 1.43 and 2.50 microg/mL, respectively; calibration curves were linear from 10 to 500 microg/mL with r2 > 0.999. Mean recoveries of the analytes were greater than 96%. In addition, a comparison with the LC method described in the 2010 Chinese Pharmacopoeia demonstrated that the developed CZE method was equally comparable with regard to linearity, sensitivity, precision, and accuracy, but the analysis time and reagent consumption were decreased. The CZE method is an effective and lower-cost alternative to the LC method.
authors.forename: Lina
authors.initials: L
authors.lastname: Xu
authors.forename: Feng
authors.initials: F
authors.lastname: Luan
authors.forename: Lijuan
authors.initials: L
authors.lastname: Wang
authors.forename: Huitao
authors.initials: H
authors.lastname: Liu
authors.forename: Yuan
authors.initials: Y
authors.lastname: Gao
mesh_terms: D000969:Antinematodal Agents / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods*; D004338:Drug Combinations; D019075:Electrophoresis, Capillary / Q000379:methods*; D007978:Levamisole / Q000737:chemistry*; D008463:Mebendazole / Q000737:chemistry*; D015394:Molecular Structure; D013607:Tablets / Q000737:chemistry
chemical_list: D000969:Antinematodal Agents; D004338:Drug Combinations; D013607:Tablets; D007978:Levamisole; D008463:Mebendazole