title: Rapid detection of terbufos in stomach contents using desorption electrospray ionization mass spectrometry.
abstract: Desorption electrospray ionization mass spectrometry (DESI-MS) is an emerging analytical technique that permits the rapid and direct analysis of biological or environmental samples under ambient conditions. Highlighting the versatility of this technique, DESI-MS has been used for the rapid detection of illicit drugs, chemical warfare agents, agricultural chemicals, and pharmaceuticals from a variety of sample matrices. In diagnostic veterinary toxicology, analyzing samples using traditional analytical instrumentation typically includes extensive sample extraction procedures, which can be time consuming and labor intensive. Therefore, efforts to expedite sample analyses are a constant goal for diagnostic toxicology laboratories. In the current report, DESI-MS was used to directly analyze stomach contents from a dog exposed to the organophosphate insecticide terbufos. The total DESI-MS analysis time required to confirm the presence of terbufos and diagnose organophosphate poisoning in this case was approximately 5 min. This highlights the potential of this analytical technique in the field of veterinary toxicology for the rapid diagnosis and detection of toxicants in biological samples.
authors.affiliation: Department of Comparative Pathobiology, School of Veterinary Medicine (Wilson, Hooser), Purdue University, West Lafayette, INAnimal Disease Diagnostic Laboratory (Wilson, Hooser), Purdue University, West Lafayette, INDepartment of Chemistry, Illinois State University, Normal, IL (Mulligan)Frankfort Animal Hospital, Frankfort, IN (Strueh)Veterinary Diagnostic & Production Animal Medicine, Ames, IA (Stevenson) wilsonc@purdue.edu.
authors.forename: Christina R
authors.initials: CR
authors.lastname: Wilson
authors.affiliation: Department of Comparative Pathobiology, School of Veterinary Medicine (Wilson, Hooser), Purdue University, West Lafayette, INAnimal Disease Diagnostic Laboratory (Wilson, Hooser), Purdue University, West Lafayette, INDepartment of Chemistry, Illinois State University, Normal, IL (Mulligan)Frankfort Animal Hospital, Frankfort, IN (Strueh)Veterinary Diagnostic & Production Animal Medicine, Ames, IA (Stevenson).
authors.forename: Christopher C
authors.initials: CC
authors.lastname: Mulligan
authors.affiliation: Department of Comparative Pathobiology, School of Veterinary Medicine (Wilson, Hooser), Purdue University, West Lafayette, INAnimal Disease Diagnostic Laboratory (Wilson, Hooser), Purdue University, West Lafayette, INDepartment of Chemistry, Illinois State University, Normal, IL (Mulligan)Frankfort Animal Hospital, Frankfort, IN (Strueh)Veterinary Diagnostic & Production Animal Medicine, Ames, IA (Stevenson).
authors.forename: Kurt D
authors.initials: KD
authors.lastname: Strueh
authors.affiliation: Department of Comparative Pathobiology, School of Veterinary Medicine (Wilson, Hooser), Purdue University, West Lafayette, INAnimal Disease Diagnostic Laboratory (Wilson, Hooser), Purdue University, West Lafayette, INDepartment of Chemistry, Illinois State University, Normal, IL (Mulligan)Frankfort Animal Hospital, Frankfort, IN (Strueh)Veterinary Diagnostic & Production Animal Medicine, Ames, IA (Stevenson).
authors.forename: Gregory W
authors.initials: GW
authors.lastname: Stevenson
authors.affiliation: Department of Comparative Pathobiology, School of Veterinary Medicine (Wilson, Hooser), Purdue University, West Lafayette, INAnimal Disease Diagnostic Laboratory (Wilson, Hooser), Purdue University, West Lafayette, INDepartment of Chemistry, Illinois State University, Normal, IL (Mulligan)Frankfort Animal Hospital, Frankfort, IN (Strueh)Veterinary Diagnostic & Production Animal Medicine, Ames, IA (Stevenson).
authors.forename: Stephen B
authors.initials: SB
authors.lastname: Hooser
keywords: Ambient mass spectrometry; desorption electrospray ionization; toxicology