title: Development and validation of an HPLC method for determination of Amikacin in water samples by solid phase extraction and pre-column derivatization.
abstract: This work presents a rapid and sensitive high performance liquid chromatography method for the determination of amikacin in water samples with solid phase extraction and pre-column derivatization. Amikacin residue was extracted from water samples with solid phase extraction cartridge. Then the extraction solution was derivatized with 4-chloro-3,5-dinitrobenzotrifluoride in the presence of triethylamine at 70°C in 20 min. The amikacin derivative was separated on a C18 column and detected by application of UV detection at 238 nm. The limit of detection is 0.2 μg/L with a signal-to-noise ratio of 3 and linearity is established over the concentration range from 0 to 500.0 μg/L. Recoveries of the amikacin in three types of water samples are from 87.5 % to 99.6 % and RSDs are 2.1 %-4.5 %. This method can be used for the quantification of amikacin residues in water samples.
authors.affiliation: College of Agriculture and Biotechnology, China Agricultural University, Beijing, China.
authors.forename: Deguang
authors.initials: D
authors.lastname: Li
authors.forename: Shun
authors.initials: S
authors.lastname: He
authors.forename: Yufang
authors.initials: Y
authors.lastname: Deng
authors.forename: Guanglong
authors.initials: G
authors.lastname: Ding
authors.forename: Hanwen
authors.initials: H
authors.lastname: Ni
authors.forename: Yongsong
authors.initials: Y
authors.lastname: Cao
mesh_terms: D000583:Amikacin / Q000032:analysis*; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000592:standards*; D004784:Environmental Monitoring / Q000379:methods*; D057230:Limit of Detection; D052616:Solid Phase Extraction; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D014874:Water Pollutants, Chemical; D000583:Amikacin