title: Effects of resveratrol on the insulin signaling pathway of obese mice.
abstract: The present study was conducted to investigate the effects of resveratrol on the insulin signaling pathway in the liver of obese mice. To accomplish this, we administered resveratrol to high fat diet-induced obese mice and examined the levels of protein phosphorylation in the liver using an antibody array. The phosphorylation levels of 10 proteins were decreased in the high fat diet and resveratrol (HFR) fed group relative to the levels in the high fat diet (HF) fed group. In contrast, the phosphorylation levels of more than 20 proteins were increased in the HFR group when compared with the levels of proteins in the HF group. Specifically, the phosphorylation levels of Akt (The308, Tyr326, Ser473) were restored to normal by resveratrol when compared with the levels in the HF group. In addition, the phosphorylation levels of IRS-1 (Ser636/Ser639), PI-3K p85-subunit α/γ (Tyr467/Tyr199), PDK1 (Ser241), GSK-3α (S21) and GSK-3 (Ser9), which are involved in the insulin signaling pathway, were decreased in the HF group, whereas the levels were restored to normal in the HFR group. Overall, the results show that resveratrol restores the phosphorylation levels of proteins involved in the insulin signaling pathway, which were decreased by a high fat diet.
authors.affiliation: Department of Toxicology & Biochemistry, College of Veterinary Medicine, Jeju National University, Jeju 690-756, Korea.
authors.forename: Hyun Ju
authors.initials: HJ
authors.lastname: Hong
authors.forename: Wonyoung
authors.initials: W
authors.lastname: Kang
authors.forename: Dong Geon
authors.initials: DG
authors.lastname: Kim
authors.forename: Dae Ho
authors.initials: DH
authors.lastname: Lee
authors.forename: Youngjae
authors.initials: Y
authors.lastname: Lee
authors.forename: Chang-Hoon
authors.initials: CH
authors.lastname: Han
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D005455:Fluorescent Antibody Technique; D007328:Insulin / Q000502:physiology*; D008099:Liver / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008820:Mice, Obese; D010766:Phosphorylation; D011506:Proteins / Q000378:metabolism; D000077185:Resveratrol; D015398:Signal Transduction / Q000187:drug effects*; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: D000893:Anti-Inflammatory Agents; D007328:Insulin; D011506:Proteins; D013267:Stilbenes; D000077185:Resveratrol
keywords: Akt phosphorylation; antibody array; insulin signaling pathway; obese mice; resveratrol