title: Validation study of a commercial aflatoxin detection kit according to the notification method in Japan.
abstract: A commercial aflatoxin detection ELISA kit, "RIDASCREEN(®) FAST Aflatoxin", was validated with corn samples naturally contaminated with aflatoxin and non-contaminated reference corn samples according to the Japanese Notification Method ShokuAnHatsu 0816-7. The trueness, intra-laboratory repeatability, intermediate precision, limit of detection and limit of quantitation were found to be 91%, 10%, 6.4%, 0.6μg/kg and 2μg/kg, respectively, and the performance of the kit was recognized as complying with all criteria in the Supplement Table of the Notification. These data suggest that this kit is useful as a simplified device to screen out negative corn samples contaminated with less than 4μg/kg.
authors.affiliation: AZmax Co. Ltd.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Morita
authors.forename: Anja
authors.initials: A
authors.lastname: Tischer
authors.forename: Bernhard
authors.initials: B
authors.lastname: Reck
authors.forename: Monika
authors.initials: M
authors.lastname: Maruhn
authors.forename: Walter
authors.initials: W
authors.lastname: Lübbe
mesh_terms: D000348:Aflatoxins / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000191:economics / Q000379:methods*; D005506:Food Contamination / Q000032:analysis; D007564:Japan; D011933:Reagent Kits, Diagnostic / Q000191:economics; D003313:Zea mays / Q000737:chemistry*
chemical_list: D000348:Aflatoxins; D011933:Reagent Kits, Diagnostic