title: NDEL1 was decreased in the CA3 region but increased in the hippocampal blood vessel network during the spontaneous seizure period after pilocarpine-induced status epilepticus.
abstract: Nuclear distribution factor E homolog like 1 (NDEL1) plays an important role in mitosis, neuronal migration, and microtubule organization during brain development by binding to disrupted-in-schizophrenia-1 (DISC1) or lissencephaly (LIS1). Although some evidence has suggested that DISC1 expression is altered in epilepsy, few studies have reported the relationship between NDEL1 and the etiology of epilepsy. In present study, we first investigated the expression of NDEL1 and its binding protein DISC1 after pilocarpine-induced epilepsy in male C57BL/6 mice. Data revealed that the mRNA and protein expression of NDEL1 and DISC1 in the whole hippocampus increased during the spontaneous seizure period after status epilepticus (SE). Interestingly, however, the expression of NDEL1 was decreased in the cornu ammonis 3 (CA3) and dentate gyrus (DG) regions. Moreover, SE also increased the number of blood vessels that fed the CA3 and DG regions of the hippocampus and increased the incidence of abnormalities in capillary network formation where NDEL1 protein was expressed positively. Meanwhile, the expression of phosphorylated ERK (p-ERK) was also increased during the spontaneous seizure period, with a similar expression pattern as NDEL1 and DISC1. Based on these results, we hypothesize that NDEL1 might interact with DISC1 to activate ERK signaling and function as a potential protective factor during the spontaneous seizure period after pilocarpine-induced SE.
authors.affiliation: Department of Neurology, Xiangya Hospital, Central South University, 87 Xiang Ya Road, Changsha, Hunan 410008, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Wu
authors.affiliation: Department of Neurology, Xiangya Hospital, Central South University, 87 Xiang Ya Road, Changsha, Hunan 410008, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Neurology, Xiangya Hospital, Central South University, 87 Xiang Ya Road, Changsha, Hunan 410008, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Shu
authors.affiliation: Department of Neurology, Xiangya Hospital, Central South University, 87 Xiang Ya Road, Changsha, Hunan 410008, China.
authors.forename: L
authors.initials: L
authors.lastname: Feng
authors.affiliation: Department of Neurology, Xiangya Hospital, Central South University, 87 Xiang Ya Road, Changsha, Hunan 410008, China.
authors.forename: L
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Department of Neurology, Xiangya Hospital, Central South University, 87 Xiang Ya Road, Changsha, Hunan 410008, China.
authors.forename: Z W
authors.initials: ZW
authors.lastname: Yue
authors.affiliation: Department of Neurology, Xiangya Hospital, Central South University, 87 Xiang Ya Road, Changsha, Hunan 410008, China.
authors.forename: Z H
authors.initials: ZH
authors.lastname: Luo
authors.affiliation: Department of Neurology, Xiangya Hospital, Central South University, 87 Xiang Ya Road, Changsha, Hunan 410008, China.
authors.forename: Z G
authors.initials: ZG
authors.lastname: Wu
authors.affiliation: Department of Neurology, Xiangya Hospital, Central South University, 87 Xiang Ya Road, Changsha, Hunan 410008, China. Electronic address: xiaobo_xy@126.com.
authors.forename: B
authors.initials: B
authors.lastname: Xiao
mesh_terms: D000818:Animals; D056654:CA3 Region, Hippocampal / Q000098:blood supply* / Q000503:physiopathology*; D002196:Capillaries / Q000503:physiopathology; D002352:Carrier Proteins / Q000378:metabolism*; D018891:Dentate Gyrus / Q000098:blood supply / Q000503:physiopathology; D004195:Disease Models, Animal; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D006624:Hippocampus / Q000098:blood supply / Q000503:physiopathology; D008297:Male; D008810:Mice, Inbred C57BL; D009419:Nerve Tissue Proteins / Q000378:metabolism; D010766:Phosphorylation; D010862:Pilocarpine; D017966:Pyramidal Cells / Q000098:blood supply / Q000503:physiopathology; D012333:RNA, Messenger / Q000378:metabolism; D012640:Seizures / Q000503:physiopathology*; D013226:Status Epilepticus / Q000503:physiopathology*
chemical_list: D002352:Carrier Proteins; C498794:Disc1 protein, mouse; C486015:Ndel1 protein, mouse; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D010862:Pilocarpine; D048049:Extracellular Signal-Regulated MAP Kinases
keywords: DISC1; NDEL1; hippocampus; p-ERK; spontaneous seizures; status epilepticus