title: Identification and characterization of a welwitindolinone alkaloid biosynthetic gene cluster in the stigonematalean Cyanobacterium Hapalosiphon welwitschii.
abstract: The identification of a 36 kb welwitindolinone (wel) biosynthetic gene cluster in Hapalosiphon welwitschii UTEX B1830 is reported. Characterization of the enzymes responsible for assembling the early biosynthetic intermediates geranyl pyrophosphate and 3-((Z)-2′-isocyanoethenyl)indole as well as a dedicated N-methyltransferase in the maturation of N-methylwelwitindolinone C isothiocyanate solidified the link between the wel pathway and welwitindolinone biosynthesis. Comparative analysis of the ambiguine and welwitindolinone biosynthetic pathways in two different organisms provided insights into the origins of diverse structures within hapalindole-type molecules.
authors.forename: Matthew L
authors.initials: ML
authors.lastname: Hillwig
authors.forename: Heather A
authors.initials: HA
authors.lastname: Fuhrman
authors.forename: Kuljira
authors.initials: K
authors.lastname: Ittiamornkul
authors.forename: Tyler J
authors.initials: TJ
authors.lastname: Sevco
authors.forename: Daniel H
authors.initials: DH
authors.lastname: Kwak
authors.forename: Xinyu
authors.initials: X
authors.lastname: Liu
mesh_terms: D053898:Biosynthetic Pathways; D000458:Cyanobacteria / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics* / Q000378:metabolism; D005798:Genes, Bacterial; D026121:Indole Alkaloids / Q000737:chemistry / Q000378:metabolism*; D005810:Multigene Family
chemical_list: D026121:Indole Alkaloids; C092268:N-methylwelwitindolinone C isothiocyanate