title: Porous protein crystals as catalytic vessels for organometallic complexes.
abstract: Porous protein crystals, which are protein assemblies in the solid state, have been engineered to form catalytic vessels by the incorporation of organometallic complexes. Ruthenium complexes in cross-linked porous hen egg white lysozyme (HEWL) crystals catalyzed the enantioselective hydrogen-transfer reduction of acetophenone derivatives. The crystals accelerated the catalytic reaction and gave different enantiomers based on the crystal form (tetragonal or orthorhombic). This method represents a new approach for the construction of bioinorganic catalysts from protein crystals.
authors.affiliation: Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510 (Japan); Institute for Integrated Cell-Material Sciences), Kyoto University, Yoshida, Sakyo-ku, Kyoto 606-8501 (Japan).
authors.forename: Hiroyasu
authors.initials: H
authors.lastname: Tabe
authors.forename: Satoshi
authors.initials: S
authors.lastname: Abe
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Hikage
authors.forename: Susumu
authors.initials: S
authors.lastname: Kitagawa
authors.forename: Takafumi
authors.initials: T
authors.lastname: Ueno
mesh_terms: D000098:Acetophenones / Q000737:chemistry; D002384:Catalysis; D003460:Crystallization; D015394:Molecular Structure; D009113:Muramidase / Q000737:chemistry / Q000378:metabolism; D009942:Organometallic Compounds / Q000138:chemical synthesis*; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry*; D012428:Ruthenium / Q000737:chemistry
chemical_list: D000098:Acetophenones; D009942:Organometallic Compounds; D011506:Proteins; D012428:Ruthenium; D009113:Muramidase; C038699:acetophenone
keywords: biocatalysis; crystal engineering; metalloenzymes; organometallic complexes; proteins