title: Assessment of WSSV prevalence and distribution of disease-resistant shrimp among the wild population of Penaeus monodon along the west coast of India.
abstract: Shrimp aquaculture is threatened by many diseases, among which white spot disease (WSD) caused by white spot syndrome virus (WSSV) is the leading one. Information related to the geographical distribution and seasonal prevalence of WSD is necessary to obtain a clear understanding of the disease biology in shrimp. Identification of WSD-resistant individual shrimp with DNA markers is also an important technique to develop better WSD-free shrimp health management. The present study aim is to estimate the occurrence of WSSV in Penaeus monodon qualitatively and quantitatively during three different seasons during the years 2011 to 2013 along the west coast of India. Additionally, the disease resistance prevalence using previously developed 71 bp microsatellite and 457 bp RAPD-SCAR DNA markers is also investigated. Samples were collected throughout the year from four locations along the west coast of India: Kochi, Kerala; Mangalore, Karnataka; Vasco-da-Gama, Goa; and Veraval, Gujarat. The results depicted that the average WSSV prevalence, as determined by the nested PCR method and taken cumulatively over the four locations, was the lowest (0%) during the post-monsoon season and the highest (31.6%) during the monsoon season. The WSD prevalence was observed to increase when the latitude was decreased along the west coast of India (from Veraval to Kochi). Out of the three different seasons, the average WSSV copy number was the highest (approximately 10(3) copies μg(-1) shrimp genomic DNA) during the monsoon season. The disease-resistant prevalence, as determined using the developed DNA markers, was found to be the highest in Vasco-da-Gama (59.5%) and the lowest in Kochi (40.9%). The present study suggests better options for the efficient collection of disease-free and disease-resistant brood stocks, which would be a more cost-effective and safer approach toward disease prevention over conventional trends of seed generation from unselected wild brood stock.
authors.affiliation: Division of Molecular Medicine, Bose Institute, P-1/12, CIT Scheme VII-M, Kolkata 700054, India.
authors.forename: Usri
authors.initials: U
authors.lastname: Chakrabarty
authors.affiliation: Division of Molecular Medicine, Bose Institute, P-1/12, CIT Scheme VII-M, Kolkata 700054, India.
authors.forename: Ajoy
authors.initials: A
authors.lastname: Mallik
authors.affiliation: Division of Molecular Medicine, Bose Institute, P-1/12, CIT Scheme VII-M, Kolkata 700054, India.
authors.forename: Debabrata
authors.initials: D
authors.lastname: Mondal
authors.affiliation: Division of Molecular Medicine, Bose Institute, P-1/12, CIT Scheme VII-M, Kolkata 700054, India.
authors.forename: Sourav
authors.initials: S
authors.lastname: Dutta
authors.affiliation: Division of Molecular Medicine, Bose Institute, P-1/12, CIT Scheme VII-M, Kolkata 700054, India. Electronic address: mandaln@rediffmail.com.
authors.forename: Nripendranath
authors.initials: N
authors.lastname: Mandal
mesh_terms: D000818:Animals; D060467:Disease Resistance; D007194:India; D033561:Penaeidae / Q000821:virology*; D015995:Prevalence; D060888:Real-Time Polymerase Chain Reaction; D012621:Seasons; D046848:White spot syndrome virus 1
keywords: DNA marker; Penaeus monodon; Real-time PCR; Specific pathogen resistant; WSD prevalence