title: Reduced itraconazole concentration and durations are successful in treating Batrachochytrium dendrobatidis infection in amphibians.
abstract: Amphibians are experiencing the greatest decline of any vertebrate class and a leading cause of these declines is a fungal pathogen, Batrachochytrium dendrobatidis (Bd), which causes the disease chytridiomycosis. Captive assurance colonies are important worldwide for threatened amphibian species and may be the only lifeline for those in critical threat of extinction. Maintaining disease free colonies is a priority of captive managers, yet safe and effective treatments for all species and across life stages have not been identified. The most widely used chemotherapeutic treatment is itraconazole, although the dosage commonly used can be harmful to some individuals and species. We performed a clinical treatment trial to assess whether a lower and safer but effective dose of itraconazole could be found to cure Bd infections. We found that by reducing the treatment concentration from 0.01-0.0025% and reducing the treatment duration from 11-6 days of 5 min baths, frogs could be cured of Bd infection with fewer side effects and less treatment-associated mortality.
authors.affiliation: School of Public Health, Tropical Medicine and Rehabilitation Sciences, James Cook University; laura.brannelly@my.jcu.edu.au.
authors.forename: Laura A
authors.initials: LA
authors.lastname: Brannelly
mesh_terms: D000818:Animals; D000935:Antifungal Agents / Q000008:administration & dosage*; D002025:Bufonidae; D008411:Chytridiomycota; D004305:Dose-Response Relationship, Drug; D004334:Drug Administration Schedule; D017964:Itraconazole / Q000008:administration & dosage*; D009181:Mycoses / Q000188:drug therapy / Q000382:microbiology / Q000662:veterinary*
chemical_list: D000935:Antifungal Agents; D017964:Itraconazole