title: Differences in the composition of the human antibody repertoire by B cell subsets in the blood.
abstract: The vast initial diversity of the antibody repertoire is generated centrally by means of a complex series of V(D)J gene rearrangement events, variation in the site of gene segment joining, and TdT catalyzed N-region addition. Although the diversity is great, close inspection has revealed distinct and unique characteristics in the antibody repertoires expressed by different B cell developmental subsets. In order to illustrate our approach to repertoire analysis, we present an in-depth comparison of V(D)J gene usage, hydrophobicity, length, DH reading frame, and amino acid usage between heavy chain repertoires expressed by immature, transitional, mature, memory IgD(+), memory IgD(-), and plasmacytes isolated from the blood of a single individual. Our results support the view that in both human and mouse, the H chain repertoires expressed by individual, developmental B cell subsets appear to differ in sequence content. Sequencing of unsorted B cells from the blood is thus likely to yield an incomplete or compressed view of what is actually happening in the immune response of the individual. Our findings support the view that studies designed to correlate repertoire expression with diseases of immune function will likely require deep sequencing of B cells sorted by subset.
authors.affiliation: Department of Microbiology, University of Alabama at Birmingham , Birmingham, AL , USA.
authors.forename: Eva Szymanska
authors.initials: ES
authors.lastname: Mroczek
authors.affiliation: Department of Molecular Biosciences, University of Texas at Austin , Austin, TX , USA.
authors.forename: Gregory C
authors.initials: GC
authors.lastname: Ippolito
authors.affiliation: Laboratory for Neonatology and Pediatric Immunology, Department of Pediatrics, Philipps-University , Marburg , Germany.
authors.forename: Tobias
authors.initials: T
authors.lastname: Rogosch
authors.affiliation: Department of Chemical Engineering, University of Texas at Austin , Austin, TX , USA ; Department of Biomedical Engineering, University of Texas at Austin , Austin, TX , USA.
authors.forename: Kam Hon
authors.initials: KH
authors.lastname: Hoi
authors.affiliation: Department of Medicine, University of Alabama at Birmingham , Birmingham, AL , USA.
authors.forename: Tracy A
authors.initials: TA
authors.lastname: Hwangpo
authors.affiliation: Department of Medicine, University of Alabama at Birmingham , Birmingham, AL , USA.
authors.forename: Marsha G
authors.initials: MG
authors.lastname: Brand
authors.affiliation: Department of Medicine, University of Alabama at Birmingham , Birmingham, AL , USA.
authors.forename: Yingxin
authors.initials: Y
authors.lastname: Zhuang
authors.affiliation: Department of Medicine, University of Alabama at Birmingham , Birmingham, AL , USA.
authors.forename: Cun Ren
authors.initials: CR
authors.lastname: Liu
authors.affiliation: Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham , Birmingham, AL , USA.
authors.forename: David A
authors.initials: DA
authors.lastname: Schneider
authors.affiliation: Laboratory for Neonatology and Pediatric Immunology, Department of Pediatrics, Philipps-University , Marburg , Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Zemlin
authors.affiliation: Department of Epidemiology, University of Alabama at Birmingham , Birmingham, AL , USA.
authors.forename: Elizabeth E
authors.initials: EE
authors.lastname: Brown
authors.affiliation: Department of Molecular Biosciences, University of Texas at Austin , Austin, TX , USA ; Department of Chemical Engineering, University of Texas at Austin , Austin, TX , USA ; Department of Biomedical Engineering, University of Texas at Austin , Austin, TX , USA.
authors.forename: George
authors.initials: G
authors.lastname: Georgiou
authors.affiliation: Department of Microbiology, University of Alabama at Birmingham , Birmingham, AL , USA ; Department of Medicine, University of Alabama at Birmingham , Birmingham, AL , USA.
authors.forename: Harry W
authors.initials: HW
authors.lastname: Schroeder
keywords: B cells subsets; CDR-H3; human antibody repertoire