title: Cytotoxic activity of N, N'-Bis (2-hydroxybenzyl) ethylenediamine derivatives in human cancer cell lines.
abstract: BACKGROUND
  Compounds containing ethylenediamine (-NCH2CH2N-) moiety are known to exhibit antimicrobial, -fungal, -bacterial, -tuberculosis and -cancer activities.
  
  
  METHODS
  In the present study, we evaluated the in vitro cytotoxic activity of N,N'-bis(2-hydroxybenzyl)- (6), N,N'-bis(5-bromo-2-hydroxybenzyl)- (7) and N,N'-bis(5-chloro-2-hydroxybenzyl) (8)- ethylenediamine dihydrochlorides; and N,N'-bis(2-hydroxybenzyl)- (9), N,N'-bis(5-bromo-2-hydroxybenzyl)- (10) and N,N'-bis(5-chloro-2-hydroxybenzyl) (11)- ethylenediamine toward human lung (A549), breast (MDA-MB-231) and prostate (PC3) cancer cell lines after 24-h treatment using crystal violet dye binding assay. Effects on the cell cycle the using flow cytometry, and mitochondrial membrane potential using rhodamine-123 florescent dye were also evaluated.
  
  
  RESULTS
  Compounds 7 and 8 exhibit cytotoxic activity, causing cell arrest at different phases of the cell cycle and loss of mitochondrial membrane potential in the above cancer cell lines.
  
  
  CONCLUSIONS
  These findings clearly demonstrate, to our knowledge for the first time, that ethylenediamine dihydrochloride salts-compounds 7 and 8-exhibit concentration-dependent cytotoxic activity towards A549, MDA-MB-231 and PC3 cancer cell lines, which may serve as a basis for future work on novel therapeutic agents.
authors.affiliation: Department of Chemistry, College Science and Technology, Florida A&M University, 219 Jones Hall, Tallahassee, FL 32307, U.S.A. musiliyu.musa@famu.edu.
authors.forename: Musiliyu A
authors.initials: MA
authors.lastname: Musa
authors.forename: Veera L D
authors.initials: VL
authors.lastname: Badisa
authors.forename: Lekan M
authors.initials: LM
authors.lastname: Latinwo
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000633:toxicity*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005029:Ethylenediamines / Q000737:chemistry / Q000633:toxicity*; D006801:Humans; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects
chemical_list: D000970:Antineoplastic Agents; D005029:Ethylenediamines; C031234:ethylenediamine
keywords: MMP; N,N’-Bis(2-hydroxybenzyl)ethylenediamine derivatives; cell cycle phase; cytotoxic activity; mitochondrial transmembrane potential