title: The effect of a moderate zinc deficiency and dietary fat source on the activity and expression of the Δ(3)Δ (2)-enoyl-CoA isomerase in the liver of growing rats.
abstract: Auxiliary enzymes participate in β-oxidation of unsaturated fatty acids. The objective of the study was to investigate the impact of a moderate zinc deficiency and a high intake of polyunsaturated fat on Δ(3)Δ(2)-enoyl-CoA isomerase (ECI) in the liver and other tissues. Five groups of eight weanling rats each were fed moderately zinc-deficient (ZD) or zinc-adequate (ZA) semisynthetic diets (7 or 50 mg Zn/kg) enriched with 22 % cocoa butter (CB) or 22 % safflower oil (SO) for 4 weeks: (1) ZD-CB, fed free choice; (2) ZA-CBR, ZA-CB diet fed in equivalent amounts consumed by the ZD-CB group; (3) ZD-SO, fed free choice; (4) ZA-SOR, ZA-SO diet fed in equivalent amounts consumed by the ZD-SO group; and (5) ZA-SO, fed free choice. Growth and Zn status markers were markedly reduced in the ZD groups. ECI activity in the liver of the animals fed the ZD- and ZA-SO diets were significantly higher (approximately 2- and 3-fold, respectively) as compared with the CB-fed animals, whereas activities in extrahepatic tissues (kidneys, heart, skeletal muscle, testes, adipose tissue) were not altered by dietary treatments. Transcript levels of the mitochondrial Eci gene in the liver did not significantly differ between ZD and ZA rats, but were 1.6-fold higher in the ZA-SO- than in the ZD-CB-fed animals (P < 0.05). It is concluded that diets enriched with safflower oil as a source high in linoleic acid induce markedly increased hepatic ECI activities and that a moderate Zn deficiency does not affect transcription of the mitochondrial Eci gene in the liver.
authors.affiliation: Dussmann Service Deutschland GmbH, Frankfurt am Main, Germany.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Justus
authors.forename: Edgar
authors.initials: E
authors.lastname: Weigand
mesh_terms: D020155:3-Hydroxybutyric Acid / Q000097:blood / Q000378:metabolism / Q000652:urine; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D004032:Diet; D004041:Dietary Fats / Q000008:administration & dosage*; D064006:Dodecenoyl-CoA Isomerase / Q000235:genetics / Q000378:metabolism*; D004435:Eating / Q000187:drug effects; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development; D008297:Male; D009929:Organ Size / Q000187:drug effects; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012450:Safflower Oil / Q000008:administration & dosage; D013385:Succinate Dehydrogenase / Q000378:metabolism; D014886:Weaning; D015032:Zinc / Q000008:administration & dosage* / Q000172:deficiency / Q000378:metabolism
chemical_list: D004041:Dietary Fats; C052387:cocoa butter; D012450:Safflower Oil; D013385:Succinate Dehydrogenase; D064006:Dodecenoyl-CoA Isomerase; D015032:Zinc; D020155:3-Hydroxybutyric Acid