title: Wild Mushrooms in Nepal: Some Potential Candidates as Antioxidant and ACE-Inhibition Sources.
abstract: Twenty-nine mushrooms collected in the mountainous areas of Nepal were analyzed for antioxidant activity by different methods, including Folin-Ciocalteu, ORAC, ABTS, and DPPH assays. Intracellular H2O2-scavenging activity was also performed on HaCaT cells. The results showed that phenolic compounds are the main antioxidant of the mushrooms. Among studied samples, Inonotus andersonii, and Phellinus gilvus exhibited very high antioxidant activity with the phenolic contents up to 310.8 and 258.7 mg GAE/g extracts, respectively. The H2O2-scavenging assay on cells also revealed the potential of these mushrooms in the prevention of oxidative stress. In term of ACE-inhibition, results showed that Phlebia tremellosa would be a novel and promising candidate for antihypertensive studies. This mushroom exhibited even higher in vitro ACE-inhibition activity than Ganoderma lingzhi, with the IC50 values of the two mushrooms being 32  μ g/mL and 2  μ g/mL, respectively. This is the first time biological activities of mushrooms collected in Nepal were reported. Information from this study should be a valuable reference for future studies on antioxidant and ACE-inhibitory activities of mushrooms.
authors.affiliation: Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan ; Department of Chemistry, Hue College of Sciences, Hue University, 77 Nguyen Hue, Hue, Vietnam.
authors.forename: Tran
authors.initials: T
authors.lastname: Hai Bang
authors.affiliation: Miyazaki Prefectural Wood Utilization Research Center, Miyazaki 885-0037, Japan.
authors.forename: Hiroto
authors.initials: H
authors.lastname: Suhara
authors.affiliation: Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan.
authors.forename: Katsumi
authors.initials: K
authors.lastname: Doi
authors.affiliation: International College of Arts and Sciences, Fukuoka Women's University, Fukuoka 813-8529, Japan.
authors.forename: Hiroya
authors.initials: H
authors.lastname: Ishikawa
authors.affiliation: Material Management Center, Kyushu University, Fukuoka 812-8581, Japan.
authors.forename: Katsuya
authors.initials: K
authors.lastname: Fukami
authors.affiliation: Plant Pathology Division, Nepal Agriculture Research Council, Khumaltar, Lalitpur, Nepal, P.O. Box. 3605, Kathmandu, Nepal.
authors.forename: Gopal Prasad
authors.initials: GP
authors.lastname: Parajuli
authors.affiliation: Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan.
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Katakura
authors.affiliation: Graduate School of Systems Life Sciences, Kyushu University, Fukuoka 812-8581, Japan.
authors.forename: Shuntaro
authors.initials: S
authors.lastname: Yamashita
authors.affiliation: Plant Pathology Division, Nepal Agriculture Research Council, Khumaltar, Lalitpur, Nepal, P.O. Box. 3605, Kathmandu, Nepal.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Watanabe
authors.affiliation: 254 Adhikari Niwas, Alka Basti, Lainchour, P.O. Box 21758, 29, Kathmandu, Nepal.
authors.forename: Mahesh Kumar
authors.initials: MK
authors.lastname: Adhikari
authors.affiliation: Plant Pathology Division, Nepal Agriculture Research Council, Khumaltar, Lalitpur, Nepal, P.O. Box. 3605, Kathmandu, Nepal.
authors.forename: Hira Kaji
authors.initials: HK
authors.lastname: Manandhar
authors.affiliation: Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan.
authors.forename: Ryuichiro
authors.initials: R
authors.lastname: Kondo
authors.affiliation: Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan.
authors.forename: Kuniyoshi
authors.initials: K
authors.lastname: Shimizu