title: Validation of 31 of the most commonly used immunohistochemical antibodies in cytology prepared using the Cellient(®) automated cell block system.
abstract: The Cellient(®) cell block system has become available as an alternative, partially automated method to create cell blocks in cytology. We sought to show a validation method for immunohistochemical (IHC) staining on the Cellient cell block system (CCB) in comparison with the formalin fixed paraffin embedded traditional cell block (TCB). Immunohistochemical staining was performed using 31 antibodies on 38 patient samples for a total of 326 slides. Split samples were processed using both methods by following the Cellient(®) manufacturer's recommendations for the Cellient cell block (CCB) and the Histogel method for preparing the traditional cell block (TCB). Interpretation was performed by three pathologists and two cytotechnologists. Immunohistochemical stains were scored as: 0/1+ (negative) and 2/3+ (positive). Inter-rater agreement for each antibody was evaluated for CCB and TCB, as well as the intra-rater agreement between TCB and CCB between observers. Interobserver staining concordance for the TCB was obtained with statistical significance (P < 0.05) in 24 of 31 antibodies. Interobserver staining concordance for the CCB was obtained with statistical significance in 27 of 31 antibodies. Intra-observer staining concordance between TCB and CCB was obtained with statistical significance in 24 of 31 antibodies tested. In conclusions, immunohistochemical stains on cytologic specimens processed by the Cellient system are reliable and concordant with stains performed on the same split samples processed via a formalin fixed-paraffin embedded (FFPE) block. The Cellient system is a welcome adjunct to cytology work-flow by producing cell block material of sufficient quality to allow the use of routine IHC.
authors.affiliation: Department of Pathology and Laboratory Medicine, Vassar Brothers Medical Center, Poughkeepsie, New York.
authors.forename: Eric
authors.initials: E
authors.lastname: Montgomery
authors.forename: Chen
authors.initials: C
authors.lastname: Gao
authors.forename: Julie
authors.initials: J
authors.lastname: de Luca
authors.forename: Jessie
authors.initials: J
authors.lastname: Bower
authors.forename: Kristropher
authors.initials: K
authors.lastname: Attwood
authors.forename: Lourdes
authors.initials: L
authors.lastname: Ylagan
mesh_terms: D000906:Antibodies / Q000276:immunology*; D001331:Automation; D003581:Cytodiagnosis / Q000379:methods; D005557:Formaldehyde; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D013048:Specimen Handling / Q000379:methods; D013194:Staining and Labeling; D054928:Validation Studies as Topic
chemical_list: D000906:Antibodies; D005557:Formaldehyde
keywords: Cellient cell block; cytology; immunohistochemistry; validation