title: Evaluation of bone formation guided by DNA/protamine complex with FGF-2 in an adult rat calvarial defect model.
abstract: DNA/protamine complex paste (D/P) and D/P complex paste with Fibroblast Growth Factor-2 (FGF-2) (D/P-FGF) were prepared to investigate their new bone formation abilities using an ∼40-week-old rat calvarial defect model. It was found that D/P could release FGF-2 proportionally in an in vitro experiment with an enzyme-linked immunosorbent assay. It was also found that aging adversely affected self-bone healing of rats by comparison with the results in a previous study using 10-week-old rats. Microcomputed tomography and histopathological examinations showed that new bone formation abilities of D/P and D/P-FGF were superior to that of the control (sham operation). Control, D/P and D/P-FGF showed newly formed bone areas of 6.7, 58.3, and 67.0%, respectively, 3 months after the operation. Moreover, it was found that FGF-2 could support the osteoanagenesis ability of D/P. It was considered that FGF-2 could play an important role in new bone formation at early stages because it induced the genes such as collagen I, CBFA, OSX, and OPN, which are initiated first in the process of osteogenesis. Therefore, D/P-FGF will be a useful injectable biomaterial with biodegradable properties for the repair of bone defects in the elderly.
authors.affiliation: Department of Oral Rehabilitation, Oral Implantology section, Fukuoka Dental College, Fukuoka, Japan.
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Shinozaki
authors.forename: Masako
authors.initials: M
authors.lastname: Toda
authors.forename: Jun
authors.initials: J
authors.lastname: Ohno
authors.forename: Minoru
authors.initials: M
authors.lastname: Kawaguchi
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Kido
authors.forename: Tadao
authors.initials: T
authors.lastname: Fukushima
mesh_terms: D020341:Absorbable Implants; D000818:Animals; D018786:Bone Substitutes / Q000737:chemistry / Q000494:pharmacology; D004247:DNA / Q000737:chemistry / Q000494:pharmacology; D004195:Disease Models, Animal; D016222:Fibroblast Growth Factor 2 / Q000737:chemistry / Q000494:pharmacology; D008297:Male; D018919:Neovascularization, Physiologic / Q000187:drug effects; D010012:Osteogenesis / Q000187:drug effects*; D011479:Protamines / Q000737:chemistry / Q000493:pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D012886:Skull / Q000293:injuries* / Q000378:metabolism / Q000473:pathology; D055114:X-Ray Microtomography
chemical_list: D018786:Bone Substitutes; D011479:Protamines; D016222:Fibroblast Growth Factor 2; D004247:DNA
keywords: DNA; bone regeneration; elderly; fibroblast growth factor-2; protamine