title: Pyruvate carboxylase as a sensitive protein biomarker for exogenous steroid chemicals.
abstract: Assessing protein responses to endocrine disrupting chemicals is critical for understanding the mechanisms of chemical action and for the assessment of hazards. In this study, the response of the liver proteome of male rare minnows (Gobiocypris rarus) treated with 17β-estradiol (E2) and females treated with 17α-methyltestosterone (MT) were analyzed. A total of 23 and 24 proteins were identified with differential expression in response to E2 and MT, respectively. Pyruvate carboxylase (PC) was the only common differentially expressed protein in both males and females after E2- and MT-treatments. The mRNA as well as the protein levels of PC were significantly down-regulated compared with that of the controls (p < 0.05). Our results suggest that endocrine disruptors interfere with genes and proteins of the TCA cycle and PC may be a sensitive biomarker of exposure to exogenous steroid chemicals in the liver of fish.
authors.affiliation: State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, 18 Shuangqing Road, Haidian District, Beijing 100085, China.
authors.forename: Xuefang
authors.initials: X
authors.lastname: Liang
authors.affiliation: Canadian Rivers Institute, Department of Biology, University of New Brunswick, Saint John, New Brunswick, Canada E2L 4L5.
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Martyniuk
authors.affiliation: Key Lab for Biotechnology of National Commission for Nationalities, College of Life Science, South Central University for Nationalities, Wuhan 430074, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Cheng
authors.affiliation: State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, 18 Shuangqing Road, Haidian District, Beijing 100085, China. Electronic address: jmzha@rcees.ac.cn.
authors.forename: Jinmiao
authors.initials: J
authors.lastname: Zha
authors.affiliation: State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, 18 Shuangqing Road, Haidian District, Beijing 100085, China. Electronic address: zjwang@rcees.ac.cn.
authors.forename: Zijian
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D003530:Cyprinidae / Q000235:genetics / Q000378:metabolism; D052244:Endocrine Disruptors / Q000633:toxicity*; D004784:Environmental Monitoring / Q000379:methods; D004958:Estradiol / Q000633:toxicity; D004997:Ethinyl Estradiol / Q000378:metabolism / Q000494:pharmacology; D005260:Female; D008297:Male; D011766:Pyruvate Carboxylase / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D015415:Biomarkers; D052244:Endocrine Disruptors; D012333:RNA, Messenger; D014874:Water Pollutants, Chemical; D004997:Ethinyl Estradiol; D004958:Estradiol; D011766:Pyruvate Carboxylase
keywords: Exogenous steroid chemicals; Protein biomarker; Pyruvate carboxylase; Rare minnow