title: [Clinical and microbiological effects of the initial periodontal therapy].
abstract: BACKGROUND
  Periodontitis is a destructive inflammatory disease of the tooth-supporting tissues, primarily caused by Gram-negative microorganisms. Thus, the primary objective of cause-related initial periodontal therapy is disruption and removal of the subgingival biofilm.
  
  
  OBJECTIVE
  The aim of this study was to evaluate the clinical and microbiological effects of the initial therapy in patients diagnosed with chronic periodontitis.
  
  
  METHODS
  Forty patients with chronic periodontitis were included in the study. As a part of the clinical assessment undertaken prior to the initial therapy, as well as one month and three months post-therapy, plaque index, gingival index, papilla bleeding index, probing pocket depth and clinical attachment level were recorded. Microbiological testing was performed prior to the initial therapy and three months after therapy. Polymerase chain reaction assays were used to determine the presence of Porphyromonas gingivalis, Tannerella forsythensis, Prevotella intermedia and Aggregatibacter actinomycetemcomitans.
  
  
  RESULTS
  All clinical parameters were significantly reduced after therapy. The prevalence of Aggregatibacter actinomycetemcomitans was reduced by 22.5%, which was a statistically significant decrease compared to the baseline. The prevalence of Porphyromonas gingivalis, Tannerella forsythensis and Prevotella intermedia tended to decrease after therapy; however, the difference did not reach statistical significance.
  
  
  CONCLUSIONS
  The results of the present study demonstrated the beneficial effects of the initial periodontal therapy on both the clinical and microbiological parameters.
authors.forename: Tanja
authors.initials: T
authors.lastname: Predin
authors.forename: Milanko
authors.initials: M
authors.lastname: Djurić
authors.forename: Jelena
authors.initials: J
authors.lastname: Mirnić
authors.forename: Ivana
authors.initials: I
authors.lastname: Gusić
authors.forename: Nadja
authors.initials: N
authors.lastname: Nikolić
authors.forename: Dubravka
authors.initials: D
authors.lastname: Marković
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Andjelković
authors.forename: Jelena
authors.initials: J
authors.lastname: Milasin
mesh_terms: D016976:Aggregatibacter actinomycetemcomitans / Q000235:genetics / Q000302:isolation & purification; D016866:Bacteroidaceae Infections / Q000628:therapy*; D055113:Chronic Periodontitis / Q000382:microbiology / Q000628:therapy*; D004269:DNA, Bacterial / Q000032:analysis*; D003774:Dental Plaque Index; D006801:Humans; D016871:Pasteurellaceae Infections / Q000628:therapy*; D057747:Periodontal Debridement; D010512:Periodontal Index; D016133:Polymerase Chain Reaction; D016966:Porphyromonas gingivalis / Q000235:genetics / Q000302:isolation & purification; D018713:Prevotella intermedia / Q000235:genetics / Q000302:isolation & purification; D016896:Treatment Outcome
chemical_list: D004269:DNA, Bacterial