title: Detection of swelling of single isolated mitochondrion with optical microscopy.
abstract: Volume regulation under osmotic loading is one of the most fundamental functions in cells and organelles. However, the effective method to detect volume changes of a single organelle has not been developed. Here, we present a novel technique for detecting volume changes of a single isolated mitochondrion in aqueous solution based on the transmittance of the light through the mitochondrion. We found that 70% and 21% of mitochondria swelled upon addition of a hypotonic solution and Ca(2+), respectively. These results show the potential of the present technique to detect the physiological volume changes of individual small organelles such as mitochondria.
authors.affiliation: Division of Biotechnology and Life Sciences, Institute of Engineering, Tokyo University of Agriculture and Technology, Nakacho 2-24-16, Koganei, Tokyo 184-8588, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Morikawa
authors.affiliation: Division of Biotechnology and Life Sciences, Institute of Engineering, Tokyo University of Agriculture and Technology, Nakacho 2-24-16, Koganei, Tokyo 184-8588, Japan.
authors.forename: Keita
authors.initials: K
authors.lastname: Kanematsu
authors.affiliation: Division of Biotechnology and Life Sciences, Institute of Engineering, Tokyo University of Agriculture and Technology, Nakacho 2-24-16, Koganei, Tokyo 184-8588, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Shibata
authors.affiliation: Division of Biotechnology and Life Sciences, Institute of Engineering, Tokyo University of Agriculture and Technology, Nakacho 2-24-16, Koganei, Tokyo 184-8588, Japan.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Haseda
authors.affiliation: Division of Mechanical System Engineering, Institute of Engineering, Tokyo University of Agriculture and Technology, Nakacho 2-24-16, Koganei, Tokyo 184-8588, Japan.
authors.forename: Norihiro
authors.initials: N
authors.lastname: Umeda
authors.affiliation: Division of Biotechnology and Life Sciences, Institute of Engineering, Tokyo University of Agriculture and Technology, Nakacho 2-24-16, Koganei, Tokyo 184-8588, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Ohta
keywords: (000.1430) Biology and medicine; (170.2655) Functional monitoring and imaging