title: The Arabidopsis protein CONSERVED ONLY IN THE GREEN LINEAGE160 promotes the assembly of the membranous part of the chloroplast ATP synthase.
abstract: The chloroplast F1Fo-ATP synthase/ATPase (cpATPase) couples ATP synthesis to the light-driven electrochemical proton gradient. The cpATPase is a multiprotein complex and consists of a membrane-spanning protein channel (comprising subunit types a, b, b', and c) and a peripheral domain (subunits α, β, γ, δ, and ε). We report the characterization of the Arabidopsis (Arabidopsis thaliana) CONSERVED ONLY IN THE GREEN LINEAGE160 (AtCGL160) protein (AtCGL160), conserved in green algae and plants. AtCGL160 is an integral thylakoid protein, and its carboxyl-terminal portion is distantly related to prokaryotic ATP SYNTHASE PROTEIN1 (Atp1/UncI) proteins that are thought to function in ATP synthase assembly. Plants without AtCGL160 display an increase in xanthophyll cycle activity and energy-dependent nonphotochemical quenching. These photosynthetic perturbations can be attributed to a severe reduction in cpATPase levels that result in increased acidification of the thylakoid lumen. AtCGL160 is not an integral cpATPase component but is specifically required for the efficient incorporation of the c-subunit into the cpATPase. AtCGL160, as well as a chimeric protein containing the amino-terminal part of AtCGL160 and Synechocystis sp. PCC6803 Atp1, physically interact with the c-subunit. We conclude that AtCGL160 and Atp1 facilitate the assembly of the membranous part of the cpATPase in their hosts, but loss of their functions provokes a unique compensatory response in each organism.
authors.affiliation: Plant Molecular Biology , Department Biology I, Ludwig-Maximilians-Universität München, 82152 Planegg-Martinsried, Germany.
authors.forename: Thilo
authors.initials: T
authors.lastname: Rühle
authors.forename: Jafar Angouri
authors.initials: JA
authors.lastname: Razeghi
authors.forename: Evgenia
authors.initials: E
authors.lastname: Vamvaka
authors.forename: Stefania
authors.initials: S
authors.lastname: Viola
authors.forename: Chiara
authors.initials: C
authors.lastname: Gandini
authors.forename: Tatjana
authors.initials: T
authors.lastname: Kleine
authors.forename: Danja
authors.initials: D
authors.lastname: Schünemann
authors.forename: Roberto
authors.initials: R
authors.lastname: Barbato
authors.forename: Peter
authors.initials: P
authors.lastname: Jahns
authors.forename: Dario
authors.initials: D
authors.lastname: Leister
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000378:metabolism*; D002734:Chlorophyll / Q000378:metabolism; D000077194:Chlorophyll A; D025222:Chloroplast Proton-Translocating ATPases / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics; D004579:Electron Transport; D005453:Fluorescence; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D007425:Intracellular Membranes / Q000201:enzymology*; D008969:Molecular Sequence Data; D046912:Multiprotein Complexes / Q000378:metabolism; D010788:Photosynthesis; D018515:Plant Leaves / Q000378:metabolism; D011485:Protein Binding; D014176:Protein Biosynthesis; D017434:Protein Structure, Tertiary; D021122:Protein Subunits / Q000378:metabolism; D016415:Sequence Alignment; D013816:Thermodynamics; D060431:Thylakoid Membrane Proteins / Q000737:chemistry / Q000378:metabolism*; D020524:Thylakoids / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D029681:Arabidopsis Proteins; C000593850:CGL160 protein, Arabidopsis; D004269:DNA, Bacterial; D046912:Multiprotein Complexes; D021122:Protein Subunits; C036483:T-DNA; D060431:Thylakoid Membrane Proteins; D002734:Chlorophyll; D025222:Chloroplast Proton-Translocating ATPases; D000077194:Chlorophyll A