title: Expression of poly(ADP-ribose) glycohydrolase in wild-type and PARG-110 knock-out retina.
abstract: Poly(ADP-ribose) (PAR) turnover is required for many cellular processes, and highly relevant for cell death and survival. This post-translational protein modification is regulated by the synthesizing enzyme poly(ADP)ribose-polymerase (PARP) and the degrading enzyme poly(ADP-ribose) glycohydrolase (PARG). Previously, PARP activity was found to be involved in photoreceptor degeneration in the rd1 mouse and in rd1-like conditions PARP-1 was the main PARP family member contributing to photoreceptor cell death. Despite the manifest role of PARP and PAR accumulation in photoreceptor cell death, the influence of PAR degradation on photoreceptor viability was still unknown. Here, we investigated the role of PARG in photoreceptor degeneration using the PARG-110 knock out mouse and report for the first time on PARG expression in wild-type and knock-out retina.
authors.affiliation: Division of Experimental Ophthalmology, Institute for Ophthalmic Research, University Eye Clinic Tübingen, Röntgenweg 11, 72076, Tübingen, Germany, aysesahaboglu@hotmail.com.
authors.forename: Ayse
authors.initials: A
authors.lastname: Sahaboglu
authors.forename: Sylvia
authors.initials: S
authors.lastname: Bolz
authors.forename: Hubert
authors.initials: H
authors.lastname: Löwenheim
authors.forename: Francois
authors.initials: F
authors.lastname: Paquet-Durand
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006026:Glycoside Hydrolases / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D018696:Neuroprotective Agents / Q000494:pharmacology; D020419:Photoreceptor Cells, Vertebrate / Q000187:drug effects / Q000473:pathology / Q000502:physiology; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases / Q000235:genetics* / Q000378:metabolism; D012160:Retina / Q000187:drug effects / Q000473:pathology / Q000502:physiology*; D012162:Retinal Degeneration / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology*
chemical_list: D004791:Enzyme Inhibitors; D018696:Neuroprotective Agents; C499321:Parp1 protein, mouse; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases; D006026:Glycoside Hydrolases; C022407:poly ADP-ribose glycohydrolase