title: Whole-cell patch-clamp recordings of Ca2+ currents from isolated neonatal mouse dorsal root ganglion (DRG) neurons.
abstract: Primary culture of sensory neurons from dorsal root ganglia (DRGs) is a widely used model for studying Ca(2+) channels. DRG neurons can be collected from neonate or adult mice; the production of cultures can take a couple of hours, and cells so derived can be used almost immediately or maintained for as long as 1 wk. This method allows the isolation of neurons for numerous experimental purposes, including whole-cell patch-clamp recording. The purpose of this protocol is to provide a description of methods commonly used for the harvest and growth of DRG neonatal neurons as well as for recording whole-cell currents through voltage-sensitive Ca(2+) channels in these cells.
authors.affiliation: Department of Cell Biology, Center for Research and Advanced Studies of the National Polytechnic Institute (Cinvestav-IPN), Mexico City, Mexico;
authors.forename: María A
authors.initials: MA
authors.lastname: Gandini
authors.forename: Alejandro
authors.initials: A
authors.lastname: Sandoval
authors.forename: Ricardo
authors.initials: R
authors.lastname: Felix
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000502:physiology*; D002469:Cell Separation / Q000379:methods; D005727:Ganglia, Spinal / Q000166:cytology*; D051379:Mice; D009474:Neurons / Q000502:physiology*; D018408:Patch-Clamp Techniques / Q000379:methods*
chemical_list: D015220:Calcium Channels; D002118:Calcium