title: Validation of a microsatellite panel for parentage testing of locally adapted and commercial goats in Brazil.
abstract: Brazilian goats are generally kept in small herds and extensive rearing systems, mainly in the northeastern region of the country. Despite production improvement in recent years, the lack of pedigree control has affected genetic progress. This study aimed to validate a panel of 16 microsatellites for parentage testing in locally adapted and commercial goats breeds raised in Brazil, as well as to compare its efficiency with the panel recommended by the Brazilian Ministry of Agriculture, Livestock and Supplies (MAPA) in 2004. The number of alleles and expected heterozygosity (He) per marker ranged from four to 18, and from 0.051 to 0.831, respectively. Using all markers, 100% of parentage cases of the validation dataset were resolved with a strict confidence level of 95%. The 16 microsatellites panel showed adequate exclusion power (99.99%) and identity accuracy (99.99%). Suggestions for improvement of the marker panel endorsed by MAPA are provided.
authors.affiliation: Pós-graduação em Ciências Animais, Universidade de Brasília, Brasília, DF, Brazil . ; Embrapa Recursos Genéticos e Biotecnologia, Brasília, DF, Brazil .
authors.forename: Elizabete Cristina
authors.initials: EC
authors.lastname: da Silva
authors.affiliation: Pós-graduação em Ciências Animais, Universidade de Brasília, Brasília, DF, Brazil . ; Departamento de Zootecnia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil .
authors.forename: Concepta Margaret
authors.initials: CM
authors.lastname: McManus
authors.affiliation: Caprileite/Associação dos Criadores de Caprinos e Ovinos de Minas Gerais, Belo Horizonte, MG, Brazil .
authors.forename: Maria Pia Souza Lima Mattos
authors.initials: MP
authors.lastname: de Paiva Guimarães
authors.affiliation: Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil .
authors.forename: Aurora M G
authors.initials: AM
authors.lastname: Gouveia
authors.affiliation: Embrapa Caprinos e Ovinos, Sobral, CE, Brazil .
authors.forename: Olivardo
authors.initials: O
authors.lastname: Facó
authors.affiliation: Faculdade de Agronomia e Medicina Veterinária, Universidade de Brasília, Brasília, DF, Brazil .
authors.forename: Daniel M
authors.initials: DM
authors.lastname: Pimentel
authors.affiliation: Pós-graduação em Ciências Animais, Universidade de Brasília, Brasília, DF, Brazil . ; Embrapa Recursos Genéticos e Biotecnologia, Brasília, DF, Brazil .
authors.forename: Alexandre Rodrigues
authors.initials: AR
authors.lastname: Caetano
authors.affiliation: Pós-graduação em Ciências Animais, Universidade de Brasília, Brasília, DF, Brazil . ; Embrapa Recursos Genéticos e Biotecnologia, Brasília, DF, Brazil . ; Embrapa Sede, Secretaria de Relações Internacionais, Brasília, DF, Brazil .
authors.forename: Samuel Rezende
authors.initials: SR
authors.lastname: Paiva
keywords: Capra hircus; animal breeding; animal genetic resources; parentage errors