title: Microsphere-based intracellular sensing of caspase-3/7 in apoptotic living cells.
abstract: A novel multifunctional probe to monitor intracellular enzymatic activity in living cells is successfully developed. Their use as accurate intracellular sensors by conjugation of an internal control (that gives an extra feature to both evaluate cellular-uptake efficiency and track probes over time) is reported. In particular, a specific application of these multifunctional microspheres as sensors of caspase-3/7 to monitor apoptosis by flow cytometry is described. The preparation of these devices together with a kinetic study towards caspase-3 and caspase-7 and their evaluation as flow cytometry probe in apoptotic living cells are reported.
authors.affiliation: School of Chemistry, University of Edinburgh, Joseph Black building, West Mains Road, Edinburgh, EH9 3JJ, UK.
authors.forename: Juan Manuel
authors.initials: JM
authors.lastname: Cárdenas-Maestre
authors.forename: Ana M
authors.initials: AM
authors.lastname: Pérez-López
authors.forename: Mark
authors.initials: M
authors.lastname: Bradley
authors.forename: Rosario M
authors.initials: RM
authors.lastname: Sánchez-Martín
mesh_terms: D017209:Apoptosis; D002232:Carbocyanines / Q000378:metabolism; D053148:Caspase 3 / Q000378:metabolism*; D053179:Caspase 7 / Q000378:metabolism*; D002470:Cell Survival; D005434:Flow Cytometry; D006367:HeLa Cells; D006801:Humans; D042541:Intracellular Space / Q000378:metabolism*; D007700:Kinetics; D008863:Microspheres; D015203:Reproducibility of Results
chemical_list: D002232:Carbocyanines; C085321:cyanine dye 5; D053148:Caspase 3; D053179:Caspase 7
keywords: caspase-3/7 sensors; flow cytometry; fluorogenic substrates; intracellular sensing; living cells; multifunctional microspheres; nanoprobes