title: Knockdown of the HDAC1 promotes the directed differentiation of bone mesenchymal stem cells into cardiomyocytes.
abstract: Failure of the directed differentiation of the transplanted stem cells into cardiomyocytes is still a major challenge of cardiac regeneration therapy. Our recent study has demonstrated that the expression of histone deacetylase 1 (HDAC1) is decreased in bone mesenchymal stem cells (BMSCs) during their differentiation into cardiomyocytes. However, the potential roles of HDAC1 in cardiac cell differentiation of BMSCs, as well as the mechanisms involved are still unclear. In current study, the expression of HDAC1 in cultured rat BMSCs is knocked down by lentiviral vectors expressing HDAC1-RNAi. The directed differentiation of BMSCs into cardiomyocytes is evaluated by the expression levels of cardiomyocyte-related genes such as GATA-binding protein 4 (GATA-4), Nirenberg, Kim gene 2 homeobox 5 (Nkx2.5), cardiac troponin T (CTnT), myosin heavy chain (MHC), and connexin-43. Compared with that in control BMSCs, the expression of these cardiomyocyte-related genes is significantly increased in these HDAC1 deficient stem cells. The results suggest that HDAC1 is involved in the cardiomyocyte differentiation of BMSCs. Knockdown of the HDAC1 may promote the directed differentiation of BMSCs into cardiomyocytes.
authors.affiliation: Department of Cardiology, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China.
authors.forename: Dong-feng
authors.initials: DF
authors.lastname: Lu
authors.affiliation: Department of Cardiology, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Cardiology, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China.
authors.forename: Zi-zhuo
authors.initials: ZZ
authors.lastname: Su
authors.affiliation: Department of Cardiology, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China.
authors.forename: Zhao-hua
authors.initials: ZH
authors.lastname: Zeng
authors.affiliation: Department of Cardiology, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China.
authors.forename: Xiao-wen
authors.initials: XW
authors.lastname: Xing
authors.affiliation: Department of Cardiology, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China.
authors.forename: Zhi-yu
authors.initials: ZY
authors.lastname: He
authors.affiliation: Department of Pharmacology and Cardiovascular Research Center, Rush Medical college of Rush University, Chicago, Illinois, United States of America.
authors.forename: Chunxiang
authors.initials: C
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000502:physiology*; D002454:Cell Differentiation / Q000235:genetics*; D002478:Cells, Cultured; D055785:Gene Knockdown Techniques / Q000379:methods; D056284:Histone Deacetylase 1 / Q000235:genetics*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D032383:Myocytes, Cardiac / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000502:physiology*
chemical_list: C515890:Hdac1 protein, rat; D056284:Histone Deacetylase 1