title: MCM10: one tool for all-Integrity, maintenance and damage control.
abstract: Minichromsome maintenance protein 10 (Mcm10) is an essential replication factor that is required for the activation of the Cdc45:Mcm2-7:GINS helicase. Mcm10's ability to bind both ds and ssDNA appears vital for this function. In addition, Mcm10 interacts with multiple players at the replication fork, including DNA polymerase-α and proliferating cell nuclear antigen with which it cooperates during DNA elongation. Mcm10 lacks enzymatic function, but instead provides the replication apparatus with an oligomeric scaffold that likely acts in the coordination of DNA unwinding and DNA synthesis. Not surprisingly, loss of Mcm10 engages checkpoint, DNA repair and SUMO-dependent rescue pathways that collectively counteract replication stress and chromosome breakage. Here, we review Mcm10's structure and function and explain how it contributes to the maintenance of genome integrity.
authors.affiliation: Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN 55455, United States.
authors.forename: Yee Mon
authors.initials: YM
authors.lastname: Thu
authors.affiliation: Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN 55455, United States. Electronic address: bieli003@umn.edu.
authors.forename: Anja-Katrin
authors.initials: AK
authors.lastname: Bielinsky
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017124:Conserved Sequence; D004249:DNA Damage; D004260:DNA Repair; D004261:DNA Replication; D006801:Humans; D064110:Minichromosome Maintenance Proteins / Q000737:chemistry / Q000502:physiology*; D009369:Neoplasms / Q000235:genetics; D011485:Protein Binding; D055503:Protein Multimerization
chemical_list: C489613:MCM10 protein, human; D064110:Minichromosome Maintenance Proteins
keywords: Cancer mutations; DNA replication; Genome integrity; Mcm10; OB-fold; STUbL