title: Effects of inhalational anaesthetics in experimental allergic asthma.
abstract: We evaluated whether isoflurane, halothane and sevoflurane attenuate the inflammatory response and improve lung morphofunction in experimental asthma. Fifty-six BALB/c mice were sensitised and challenged with ovalbumin and anaesthetised with isoflurane, halothane, sevoflurane or pentobarbital sodium for one hour. Lung mechanics and histology were evaluated. Gene expression of pro-inflammatory (tumour necrosis factor-α), pro-fibrogenic (transforming growth factor-β) and pro-angiogenic (vascular endothelial growth factor) mediators, as well as oxidative process modulators, were analysed. These modulators included nuclear factor erythroid-2 related factor 2, sirtuin, catalase and glutathione peroxidase. Isoflurane, halothane and sevoflurane reduced airway resistance, static lung elastance and atelectasis when compared with pentobarbital sodium. Sevoflurane minimised bronchoconstriction and cell infiltration, and decreased tumour necrosis factor-α, transforming growth factor-β, vascular endothelial growth factor, sirtuin, catalase and glutathione peroxidase, while increasing nuclear factor erythroid-2-related factor 2 expression. Sevoflurane down-regulated inflammatory, fibrogenic and angiogenic mediators, and modulated oxidant-antioxidant imbalance, improving lung function in this model of asthma.
authors.affiliation: Laboratory of Pulmonary Investigation, Carlos Chagas Filho Biophysics Institute, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil; Brazilian National Cancer Institute - INCa, and Ipanema Federal Hospital, Ministry of Health, Rio de Janeiro, Brazil.
authors.forename: S M
authors.initials: SM
authors.lastname: Burburan
authors.forename: J D
authors.initials: JD
authors.lastname: Silva
authors.forename: S C
authors.initials: SC
authors.lastname: Abreu
authors.forename: C S
authors.initials: CS
authors.lastname: Samary
authors.forename: I H L
authors.initials: IH
authors.lastname: Guimarães
authors.forename: D G
authors.initials: DG
authors.lastname: Xisto
authors.forename: M M
authors.initials: MM
authors.lastname: Morales
authors.forename: P R M
authors.initials: PR
authors.lastname: Rocco
mesh_terms: D018685:Anesthetics, Inhalation / Q000494:pharmacology / Q000627:therapeutic use*; D000818:Animals; D001249:Asthma / Q000188:drug therapy* / Q000503:physiopathology; D016084:Bronchoconstriction / Q000187:drug effects; D008168:Lung / Q000503:physiopathology; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D018685:Anesthetics, Inhalation