title: Histopathology of cotton bollworm midgut infected with Helicoverpa armigera cytoplasmic polyhedrosis virus.
abstract: This research was carried out to examine cytopathological effects of Helicoverpa armigera Cytoplasmic polyhedrosis virus (HaCPV) on infected midgut cotton bollworm (Helicoverpa armigera) using transmission and scanning electron microscope. The symptoms on infected host larvae of the host, compared with healthy ones, were getting swollen with milky-white and fragile Histopathological examinations showed infection with HaCPV small polyhedral inclusion bodies (PIB) after 1 or 2 days which were observed in columnar cells of midgut. Virions were partially or completely occupied in a polyhedral matrix to form polyhedral inclusion bodies (PIB) at periphery of virogenic stroma. PIBs were measured 0.5 to 3.5 μm and virions about 46 nm in diameter. Microvilli of infected columnar cells were affected and degenerated immediately prior to rupture of the cell. Some infected columnar cells ruptured to release PIB into the gut lumen 3 days after infection. In addition, PIB were found in goblet cells, 5 or 6 days after infection. Infected goblet cells degenerate to such an extent that only a few of the original microvillus-like cytoplasmic projections and cell organells were left. These cytopathic effects caused in the midgut by HaCPV on cotton bollworm larvae are essentially similar to those have been reported for lepidoperan and dipteran infection by CPV.
authors.affiliation: Department of Biological Control, Iranian Research Institute of Plant Protection, Tehran, Iran. ; Department of Entomology, College of Agronomy and Biotechnology, China Agricultural University, Beijing, China.
authors.forename: Rasoul
authors.initials: R
authors.lastname: Marzban
authors.affiliation: Department of Entomology, College of Agronomy and Biotechnology, China Agricultural University, Beijing, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: He
authors.affiliation: Department of Entomology, College of Agronomy and Biotechnology, China Agricultural University, Beijing, China.
authors.forename: Qingwen
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Entomology, College of Agronomy and Biotechnology, China Agricultural University, Beijing, China.
authors.forename: Xiao Xia
authors.initials: XX
authors.lastname: Liu
mesh_terms: D000818:Animals; D041981:Gastrointestinal Tract / Q000473:pathology; D006651:Histocytochemistry; D007814:Larva / Q000821:virology; D007915:Lepidoptera / Q000821:virology*; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D012087:Reoviridae / Q000254:growth & development*
keywords: HaCPV; Helicoverpa armigera; cytoplasmic polyhedrosis virus; histopathology