title: Lignin, mitochondrial family, and photorespiratory transporter classification as case studies in using co-expression, co-response, and protein locations to aid in identifying transport functions.
abstract: Whole genome sequencing and the relative ease of transcript profiling have facilitated the collection and data warehousing of immense quantities of expression data. However, a substantial proportion of genes are not yet functionally annotated a problem which is particularly acute for transport proteins. In Arabidopsis, for example, only a minor fraction of the estimated 700 intracellular transporters have been identified at the molecular genetic level. Furthermore it is only within the last couple of years that critical genes such as those encoding the final transport step required for the long distance transport of sucrose and the first transporter of the core photorespiratory pathway have been identified. Here we will describe how transcriptional coordination between genes of known function and non-annotated genes allows the identification of putative transporters on the premise that such co-expressed genes tend to be functionally related. We will additionally extend this to include the expansion of this approach to include phenotypic information from other levels of cellular organization such as proteomic and metabolomic data and provide case studies wherein this approach has successfully been used to fill knowledge gaps in important metabolic pathways and physiological processes.
authors.affiliation: Department 1 (Willmitzer), Central Metabolism, Max Planck Institute for Plant Physiology Potsdam, Germany.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Tohge
authors.affiliation: Department 1 (Willmitzer), Central Metabolism, Max Planck Institute for Plant Physiology Potsdam, Germany.
authors.forename: Alisdair R
authors.initials: AR
authors.lastname: Fernie
keywords: co-expression network; informatic identification; plant metabolism; transporters