title: Ligand binding assay critical reagents and their stability: recommendations and best practices from the Global Bioanalysis Consortium Harmonization Team.
abstract: The L4 Global Harmonization Team on reagents and their stability focused on the management of critical reagents for pharmacokinetic, immunogenicity, and biomarker ligand binding assays. Regulatory guidance recognizes that reagents are important for ligand binding assays but do not address numerous aspects of critical reagent life cycle management. Reagents can be obtained from external vendors or developed internally, but regardless of their source, there are numerous considerations for their reliable long-term use. The authors have identified current best practices and provided recommendations for critical reagent lot changes, stability management, and documentation.
authors.affiliation: Pfizer, Inc., Building 220, MS 8220-2468, 600 Eastern Point Road, Groton, Connecticut, 06340, USA, lindsay.king@pfizer.com.
authors.forename: Lindsay E
authors.initials: LE
authors.lastname: King
authors.forename: Esme
authors.initials: E
authors.lastname: Farley
authors.forename: Mami
authors.initials: M
authors.lastname: Imazato
authors.forename: Jeannine
authors.initials: J
authors.lastname: Keefe
authors.forename: Masood
authors.initials: M
authors.lastname: Khan
authors.forename: Mark
authors.initials: M
authors.lastname: Ma
authors.forename: K Susanne
authors.initials: KS
authors.lastname: Pihl
authors.forename: Priya
authors.initials: P
authors.lastname: Sriraman
mesh_terms: D015415:Biomarkers; D004282:Documentation; D006801:Humans; D007202:Indicators and Reagents / Q000737:chemistry* / Q000592:standards* / Q000600:supply & distribution; D008024:Ligands; D010599:Pharmacokinetics; D012015:Reference Standards
chemical_list: D015415:Biomarkers; D007202:Indicators and Reagents; D008024:Ligands