title: Role of myeloid-derived suppressor cells in mouse pre-sensitized cardiac transplant model.
abstract: Harness of sensitized transplantation remains a clinical challenge particularly in parallel with prolonged cold ischemia time (PCI)-mediated injury. Our present study was to test the role of myeloid-derived suppressor cells (MDSCs) in mouse pre-sensitized transplantation. Our findings revealed that CD11b+Gr1(low) MDSC was shown to have strong suppressive activity. MDSCs subsets from the tolerated mice exhibited higher suppressive capacities compared with counterparts from naive (untreated) mice. Depletion of Tregs could not affect splenic CD11b+Gr1(-low) MDSC frequency, but increase peripheral and intragraft CD11b+Gr1(-low) frequency. Intriguingly, boost of Tregs remarkably caused an increase of CD11b+Gr1(-low) frequency in the graft, peripheral blood, and spleen. Furthermore, peripheral CD11b+Gr1(-low) cells were massively accumulated at the early stage when allogeneic immune response was enhanced. Taken together, MDSCs could prevent grafts from PCI-mediated injury independent on Tregs in the pre-sensitized transplant recipients. Utilization of MDSC subset particularly CD11b+Gr1(-low) might provide a novel insight into improving graft outcome under such clinical scenarios.
authors.affiliation: Department of Surgery and Medicine, Transplant International Research Centre (TIRC), Second Affiliated Hospital of School of Medicine, Zhejiang University, Hangzhou City, People's Republic of China; Departments of Medicine, Transplant Institute, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA. Electronic address: weihua.gong@gmail.com.
authors.forename: Weihua
authors.initials: W
authors.lastname: Gong
authors.affiliation: Department of Surgery and Medicine, Transplant International Research Centre (TIRC), Second Affiliated Hospital of School of Medicine, Zhejiang University, Hangzhou City, People's Republic of China.
authors.forename: Fangmin
authors.initials: F
authors.lastname: Ge
authors.affiliation: Center for Stem Cell and Translational Medicine, School of Life Sciences, Anhui University, Hefei City, People's Republic of China.
authors.forename: Dahai
authors.initials: D
authors.lastname: Liu
authors.affiliation: Departments of Medicine, Transplant Institute, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Hemotology/Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
authors.forename: Fangbing
authors.initials: F
authors.lastname: Liu
authors.affiliation: Department of Internal Medicine, College of Medicine, Yonsei University, Seoul, Korea.
authors.forename: Beom Seok
authors.initials: BS
authors.lastname: Kim
authors.affiliation: Departments of Medicine, Transplant Institute, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
authors.forename: Tao
authors.initials: T
authors.lastname: Huang
authors.affiliation: Departments of Medicine, Transplant Institute, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
authors.forename: Maria
authors.initials: M
authors.lastname: Koulmanda
authors.affiliation: Departments of Medicine, Transplant Institute, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA. Electronic address: srobson@bidmc.harvard.edu.
authors.forename: Simon C
authors.initials: SC
authors.lastname: Robson
authors.affiliation: Departments of Medicine, Transplant Institute, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
authors.forename: Terry B
authors.initials: TB
authors.lastname: Strom
mesh_terms: D000818:Animals; D039481:CD11b Antigen / Q000378:metabolism; D002452:Cell Count; D050377:Cold Ischemia / Q000009:adverse effects; D004195:Disease Models, Animal; D006085:Graft Survival / Q000276:immunology; D016027:Heart Transplantation; D016130:Immunophenotyping; D007165:Immunosuppression Therapy; D008297:Male; D051379:Mice; D022423:Myeloid Cells / Q000276:immunology* / Q000378:metabolism; D019707:Receptors, Chemokine / Q000378:metabolism; D016038:Skin Transplantation; D013154:Spleen / Q000166:cytology / Q000276:immunology
chemical_list: D039481:CD11b Antigen; C503133:Gr-1 protein, mouse; D019707:Receptors, Chemokine
keywords: Myeloid-derived suppressor cells; Prolonged cold ischemia time; Regulatory T cells; Sensitization