title: Gestational diabetes mellitus (GDM) decreases butyrylcholinesterase (BChE) activity and changes its relationship with lipids.
abstract: Many conditions interfere with butyrylcholinesterase (BChE) activity, e.g., pregnancy or presence of the BCHE gene variant -116A can decrease activity whereas obesity and types I and II diabetes mellitus can increase activity. In this study, we examined BChE activity, -116A and 1615A BCHE gene variants, and anthropometric and biochemical variables associated with diabetes in patients with gestational diabetes mellitus (GDM) and in healthy pregnant women. BChE activity was measured spectrophotometrically using propionylthiocholine as substrate and genotyping of the -116 and 1615 sites of the BCHE gene was done with a TaqMan SNP genotyping assay. Three groups were studied: 150 patients with GDM, 295 healthy pregnant women and 156 non-pregnant healthy women. Mean BChE activity was significantly lower in healthy pregnant women than in women from the general population and was further reduced in GDM patients. BChE activity was significantly reduced in carriers of -116A in GDM patients and healthy pregnant women. Although GDM patients had a significantly higher mean body mass index (BMI) and triglycerides than healthy pregnant women, they had lower mean BChE activity, suggesting that the lowering effect of GDM on BChE activity was stronger than the characteristic enhancing effect of increased BMI and triglycerides.
authors.affiliation: Paboratório de Polimorfismos e Ligação, Departamento de Genética, Universidade Federal do Paraná, Curitiba, PR, Brazil .
authors.forename: Larissa O
authors.initials: LO
authors.lastname: Guimarães
authors.affiliation: Paboratório de Polimorfismos e Ligação, Departamento de Genética, Universidade Federal do Paraná, Curitiba, PR, Brazil .
authors.forename: Fabiana A
authors.initials: FA
authors.lastname: de Andrade
authors.affiliation: Paboratório de Polimorfismos e Ligação, Departamento de Genética, Universidade Federal do Paraná, Curitiba, PR, Brazil .
authors.forename: Gleyse F
authors.initials: GF
authors.lastname: Bono
authors.affiliation: Paboratório de Polimorfismos e Ligação, Departamento de Genética, Universidade Federal do Paraná, Curitiba, PR, Brazil .
authors.forename: Thaís E
authors.initials: TE
authors.lastname: Setoguchi
authors.affiliation: Paboratório de Polimorfismos e Ligação, Departamento de Genética, Universidade Federal do Paraná, Curitiba, PR, Brazil .
authors.forename: Mariana B
authors.initials: MB
authors.lastname: Brandão
authors.affiliation: Paboratório de Polimorfismos e Ligação, Departamento de Genética, Universidade Federal do Paraná, Curitiba, PR, Brazil .
authors.forename: Eleidi A
authors.initials: EA
authors.lastname: Chautard-Freire-Maia
authors.affiliation: Laboratório de Bioquímica Clínica, Setor de Ciências da Saúde, Universidade Federal do Paraná, Curitiba, PR, Brazil .
authors.forename: Izabella C R
authors.initials: IC
authors.lastname: Dos Santos
authors.affiliation: Laboratório de Bioquímica Clínica, Setor de Ciências da Saúde, Universidade Federal do Paraná, Curitiba, PR, Brazil .
authors.forename: Geraldo
authors.initials: G
authors.lastname: Picheth
authors.affiliation: Unidade de Diabetes do Serviço de Endocrinologia e Metabolismo, Hospital de Clínicas, Universidade Federal do Paraná, Curitiba, PR, Brazil .
authors.forename: Ana Cristina R de A
authors.initials: AC
authors.lastname: Faria
authors.affiliation: Unidade de Diabetes do Serviço de Endocrinologia e Metabolismo, Hospital de Clínicas, Universidade Federal do Paraná, Curitiba, PR, Brazil .
authors.forename: Rosângela R
authors.initials: RR
authors.lastname: Réa
authors.affiliation: Paboratório de Polimorfismos e Ligação, Departamento de Genética, Universidade Federal do Paraná, Curitiba, PR, Brazil .
authors.forename: Ricardo L R
authors.initials: RL
authors.lastname: Souza
authors.affiliation: Paboratório de Polimorfismos e Ligação, Departamento de Genética, Universidade Federal do Paraná, Curitiba, PR, Brazil .
authors.forename: Lupe
authors.initials: L
authors.lastname: Furtado-Alle
keywords: 1615A variant; butyrylcholinesterase (BChE); gestational diabetes mellitus; −116A variant