title: Binuclear copper complexes: synthesis, X-ray structure and interaction study with nucleotide/protein by in vitro biochemical and electrochemical analysis.
abstract: Two new, binuclear copper(II) hydrazone complexes have been synthesized and characterized by various physico-chemical techniques including single crystal X-ray diffraction. Interaction of these complexes with nucleotide and protein were analyzed by in vitro biochemical and electrochemical analysis. Both the complexes exhibited intercalative mode of binding with DNA. Further, gel electrophoresis assay demonstrated the ability of the complexes to cleave the supercoiled pBR322 plasmid DNA to nicked circular DNA form. Cytotoxicity of the complexes performed against a panel of cancer cell lines and a normal cell line proved that these complexes are potentially cytotoxic against the cancerous cell lines, particularly with IC50 as low as 0.7 μM against HeLa cell line.
authors.affiliation: Inorganic and Nanomaterials Research Laboratory, Department of Chemistry, Bharathiar University, Coimbatore 641 046, India.
authors.forename: M
authors.initials: M
authors.lastname: Alagesan
authors.affiliation: Department of Chemistry, Texas A&M University, College Station, TX 77843, USA.
authors.forename: N S P
authors.initials: NS
authors.lastname: Bhuvanesh
authors.affiliation: Inorganic and Nanomaterials Research Laboratory, Department of Chemistry, Bharathiar University, Coimbatore 641 046, India. Electronic address: dharmaraj@buc.edu.in.
authors.forename: N
authors.initials: N
authors.lastname: Dharmaraj
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D002417:Cattle; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003300:Copper / Q000737:chemistry*; D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D055664:Electrochemical Techniques; D006367:HeLa Cells; D006801:Humans; D008958:Models, Molecular; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D012709:Serum Albumin / Q000737:chemistry*; D013329:Structure-Activity Relationship
chemical_list: D000970:Antineoplastic Agents; D009942:Organometallic Compounds; D012709:Serum Albumin; D003300:Copper; D004247:DNA
keywords: Binuclear copper complex; Cytotoxicity; Nucleotide and protein interaction