title: Expression of a functional recombinant oleosin-human hyaluronidase hPH-20 fusion in Arabidopsis thaliana.
abstract: The use of plants as expression systems for the production of recombinant proteins has distinct advantages, such as safety, ease, low cost and high yields. A plant binary expression vector, pOTBar-hPH20, containing an oleosin-hPH20 fusion gene and a Basta selection marker gene was constructed and introduced into Arabidopsis thaliana via the floral dip method. Transformed A. thaliana seed lines were obtained and analyzed by PCR. The PCR results indicated that oleosin-hPH20 fusion gene was integrated into the A. thaliana genome. The oleosin-hPH20 fusion protein was detected by SDS-PAGE and Western blot analysis. The oleosin-hPH20 fusion protein was expressed and had good antigenicity in the transgenic A. thaliana seeds. An enzyme assay suggested that the recombinant oleosin-hPH20 fusion protein had hyaluronidase activity.
authors.affiliation: Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun 130118, China.
authors.forename: Hongrui
authors.initials: H
authors.lastname: Li
authors.affiliation: Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun 130118, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.affiliation: Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun 130118, China.
authors.forename: Yubin
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun 130118, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Guan
authors.affiliation: Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun 130118, China.
authors.forename: LinNa
authors.initials: L
authors.lastname: Du
authors.affiliation: Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun 130118, China.
authors.forename: YongXin
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun 130118, China.
authors.forename: Wenhui
authors.initials: W
authors.lastname: Wang
authors.affiliation: Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun 130118, China.
authors.forename: Lihao
authors.initials: L
authors.lastname: Wang
authors.affiliation: Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun 130118, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Li
authors.affiliation: Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun 130118, China. Electronic address: changjiang10@hotmail.com.
authors.forename: Chao
authors.initials: C
authors.lastname: Jiang
authors.affiliation: Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun 130118, China. Electronic address: xiaokunli@163.net.
authors.forename: Xiaokun
authors.initials: X
authors.lastname: Li
mesh_terms: D017360:Arabidopsis / Q000235:genetics; D029681:Arabidopsis Proteins / Q000096:biosynthesis* / Q000235:genetics; D015815:Cell Adhesion Molecules / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D006821:Hyaluronoglucosaminidase / Q000096:biosynthesis* / Q000235:genetics; D030821:Plants, Genetically Modified; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000235:genetics; D012639:Seeds / Q000235:genetics
chemical_list: D029681:Arabidopsis Proteins; D015815:Cell Adhesion Molecules; D011993:Recombinant Fusion Proteins; C079353:oleosin protein, Arabidopsis; D006821:Hyaluronoglucosaminidase; C066852:hyaluronidase PH-20
keywords: Arabidopsis thaliana; Hyaluronidase; Oil body; Oleosin; hPH20