title: [Study of the biological characteristics of p75 neurotrophin receptor positive tongue squamous cell carcinoma cells].
abstract: OBJECTIVE
  To study the biological characteristics of p75 neurotrophin receptor positive (p75(NTR+)) tongue squamous cell carcinoma cells which were separated by flow cytometry cell sorting.
  
  
  METHODS
  To determine the biological characteristics of p75(NTR+) cells which were separated from Tca-8113 and Cal-27 tongue squamous cell carcinoma cells by flow cytometry cell sorting, including study the capacity of cloning, 3-(4,5)-demethylthiazo(z-y1)-3,5-diphenytetrazoliumromide (MTT) assay, wound healing assay. p75(NTR+) cells with non-sorted cells were as control group.
  
  
  RESULTS
  In Tca-8113 and Cal-27 tongue squamous cell carcinoma cell lines, the percentage of p75(NTR+) cells were 3.1% and 1.9%. Compared with p75(NTR+) cells with non-sorted cells, p75(NTR+) cells possess higher capacity of cloning (Tca-8113, P=0.024; Cal-27, P=0.009). The percentage of p75(NTR+) cells of the progeny cells generated from monoclonal p75(NTR+) cells decreased to 14.5% (Tca-8113) and 5.8% (Cal-27) after cultured two weeks. p75(NTR+) cells possessed higher proliferation ability and higher metastasis ability than non-sorted cells.
  
  
  CONCLUSIONS
  p75(NTR+) cells isolated from tongue squamous cell carcinoma have the characteristics of cancer stem cells.
authors.forename: Dongdong
authors.initials: D
authors.lastname: Tong
authors.forename: Fenghe
authors.initials: F
authors.lastname: Zhang
authors.forename: Yao
authors.initials: Y
authors.lastname: Yao
authors.forename: Zhaotao
authors.initials: Z
authors.lastname: Zhang
authors.forename: Jinbing
authors.initials: J
authors.lastname: Wang
authors.forename: Qing
authors.initials: Q
authors.lastname: Li
authors.forename: Xinlian
authors.initials: X
authors.lastname: Zhang
mesh_terms: D002294:Carcinoma, Squamous Cell; D006801:Humans; D009369:Neoplasms; D009419:Nerve Tissue Proteins; D017475:Receptors, Nerve Growth Factor; D014062:Tongue Neoplasms
chemical_list: C484065:NGFR protein, human; D009419:Nerve Tissue Proteins; D017475:Receptors, Nerve Growth Factor