title: Evidence-based medicinal value of Rudbeckia hirta L. flowers.
abstract: A phytochemical investigation on the 5-lipoxygenase (5-LOX) inhibitory methanolic extract of Rudbeckia hirta L. flowers yielded 10 phenolic metabolites, including three phenolic acids, two phenolic acid esters, four flavonol glycosides and a trimethylated flavonol. The structures of the isolated metabolites were determined on the basis of spectroscopic analyses and by comparison with the literature data. Seven of these metabolites were isolated for the first time from the genus Rudbeckia. The in vitro 5-LOX inhibitory, immunomodulatory and antioxidant (oxygen radical absorbance capacity) activities of the isolated compounds were evaluated, and the results provided a new scientific evidence for the ethnopharmacological use of the herb in inflammatory conditions.
authors.affiliation: a Department of Pharmacognosy , Faculty of Pharmacy, Mansoura University (MU) , Mansoura 35516 , Egypt.
authors.forename: Botros R
authors.initials: BR
authors.lastname: Michael
authors.forename: Sahar R
authors.initials: SR
authors.lastname: Gedara
authors.forename: Mohamed M
authors.initials: MM
authors.lastname: Amer
authors.forename: Lesley
authors.initials: L
authors.lastname: Stevenson
authors.forename: Atallah F
authors.initials: AF
authors.lastname: Ahmed
mesh_terms: D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D001094:Arachidonate 5-Lipoxygenase / Q000187:drug effects*; D044948:Flavonols / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D035264:Flowers / Q000737:chemistry*; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D007155:Immunologic Factors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D016859:Lipoxygenase Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D036582:Rudbeckia / Q000737:chemistry*
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D044948:Flavonols; D006027:Glycosides; D007155:Immunologic Factors; D016859:Lipoxygenase Inhibitors; D010636:Phenols; D010936:Plant Extracts; D001094:Arachidonate 5-Lipoxygenase
keywords: 5-LOX inhibition; ORAC; Rudbeckia hirta; antiinflammatory; antioxidant; immunomodulatory; methylated flavonols