title: Sensitive detection of polynucleotide kinase using rolling circle amplification-induced chemiluminescence.
abstract: We develop a new method for the sensitive detection of polynucleotide kinase (PNK) using rolling circle amplification-induced chemiluminescence. This method exhibits high sensitivity with a detection limit of 2.20 × 10(-4) U mL(-1), which is superior to most reported approaches. Moreover, this method can be used to screen both the inhibitors and the activators of PNK, and can be further applied for real sample analysis.
authors.affiliation: Single-Molecule Detection and Imaging Laboratory, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China. zhangcy@siat.ac.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Tang
authors.forename: Guichi
authors.initials: G
authors.lastname: Zhu
authors.forename: Chun-yang
authors.initials: CY
authors.lastname: Zhang
mesh_terms: D004789:Enzyme Activation / Q000502:physiology; D057809:HEK293 Cells; D006801:Humans; D057230:Limit of Detection; D008163:Luminescent Measurements / Q000379:methods*; D011116:Polynucleotide 5'-Hydroxyl-Kinase / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D011116:Polynucleotide 5'-Hydroxyl-Kinase