title: Maize (Zea mays L.) seedling leaf nuclear proteome and differentially expressed proteins between a hybrid and its parental lines.
abstract: To better understand the underlying molecular basis of leaf development in maize, a reference map of nuclear proteins in basal region of seedling leaf was established using a combination of 2DE and MALDI-TOF-MS. In total, 441 reproducible protein spots in nuclear proteome of maize leaf basal region were detected with silver staining in a pH range of 3-10, among which 203 spots corresponding to 163 different proteins were identified. As expected, proteins implicated in RNA and protein-associated functions were overrepresented in nuclear proteome. Remarkably, a high percentage (10%) of proteins was identified to be involved in cell division and growth. In addition, comparative nuclear proteomic analysis in leaf basal region of highly heterotic hybrid Mo17/B73 and its parental lines was also performed and 52 of 445 (11.69%) detected protein spots were differentially expressed between the hybrid and its parental lines, among which 16 protein spots displayed nonadditively expressed pattern. These results indicated that hybridization between two parental lines can cause changes in the expression of a variety of nuclear proteins, which may be responsible for the observed leaf size heterosis.
authors.affiliation: State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization (MOE), Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing, China; National Plant Gene Research Centre (Beijing), Beijing, China.
authors.forename: Baojian
authors.initials: B
authors.lastname: Guo
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Chen
authors.forename: Chuan
authors.initials: C
authors.lastname: Li
authors.forename: Tianya
authors.initials: T
authors.lastname: Wang
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
authors.forename: Bo
authors.initials: B
authors.lastname: Wang
authors.forename: Sha
authors.initials: S
authors.lastname: Hu
authors.forename: Xiaofen
authors.initials: X
authors.lastname: Du
authors.forename: Hongyan
authors.initials: H
authors.lastname: Xing
authors.forename: Xiao
authors.initials: X
authors.lastname: Song
authors.forename: Yingyin
authors.initials: Y
authors.lastname: Yao
authors.forename: Qixin
authors.initials: Q
authors.lastname: Sun
authors.forename: Zhongfu
authors.initials: Z
authors.lastname: Ni
mesh_terms: D001947:Breeding; D009687:Nuclear Proteins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D010641:Phenotype; D018515:Plant Leaves / Q000737:chemistry*; D010940:Plant Proteins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D020543:Proteome; D036226:Seedlings / Q000737:chemistry*; D003313:Zea mays / Q000737:chemistry* / Q000145:classification*
chemical_list: D009687:Nuclear Proteins; D010940:Plant Proteins; D020543:Proteome
keywords: Differentially expressed protein; Heterosis; Maize; Nonadditivity; Nuclear proteome; Plant proteomics