title: Solid lipid nanoparticles regulate functional assortment of mouse mesenchymal stem cells.
abstract: A rapid decline in self-renewability, viability and function, of isolated stem cells are major hurdles in developing cell based therapies. There has been an increasing interest towards identifying a support material for maintaining stem cell features of the isolated cells. Pioneering observations of the present paper, demonstrate functionally diverse potential of Solid Lipid Nanoparticles (SLNs) in deciding the fate & behavior of mouse mesenchymal stem cell. The evidences are provided to show the dual nature of the SLNs for being a scaffold for the stem cell attachment, to retain stemness, and as reagent for inducing stem cell differentiation. Scanning electron microscopic examinations together with expression analysis were used to conform to such observations. Results of the study thus suggest that Solid lipid nanoparticles can be used as a good support material when functionalized to achieve adhesive properties and as a molecular paradigm for studying the adipocytic differentiation. We envisage a new role of SLNs towards regulating stem cell character by orchestrating the structural alignment during preparation of Solid lipid nanoparticles.
authors.affiliation: Centre for Stem Cell & Tissue Engineering, Panjab University, Departments of 3 Biochemistry & 5 Biotechnology , Panjab University.
authors.forename: S
authors.initials: S
authors.lastname: Chabra
authors.affiliation: Centre for Stem Cell & Tissue Engineering, Panjab University, Departments of 3 Biochemistry & 5 Biotechnology , Panjab University.
authors.forename: M
authors.initials: M
authors.lastname: Ranjan
authors.affiliation: University Institute of Pharmaceutical Sciences (UIPS), Panjab University , Sector-14, Chandiagarh-160014, India.
authors.forename: R
authors.initials: R
authors.lastname: Bhandari
authors.affiliation: Departments of Biochemistry, Panjab University , Sector-14, Chandiagarh-160014, India.
authors.forename: T
authors.initials: T
authors.lastname: Kaur
authors.affiliation: Departments of Biochemistry, Panjab University , Sector-14, Chandiagarh-160014, India.
authors.forename: M
authors.initials: M
authors.lastname: Aggrawal
authors.affiliation: Departments of Bioinformatics , DAVC Sector-10, Chandigarh, India.
authors.forename: V
authors.initials: V
authors.lastname: Puri
authors.affiliation: Departments of Biochemistry, Panjab University , Sector-14, Chandiagarh-160014, India.
authors.forename: N
authors.initials: N
authors.lastname: Mahajan
authors.affiliation: University Institute of Pharmaceutical Sciences (UIPS), Panjab University , Sector-14, Chandiagarh-160014, India.
authors.forename: I P
authors.initials: IP
authors.lastname: Kaur
authors.affiliation: Centre for Stem Cell & Tissue Engineering, Panjab University, Departments of 3 Biochemistry & 5 Biotechnology , Panjab University.
authors.forename: S
authors.initials: S
authors.lastname: Puri
authors.affiliation: Departments of Biotechnology, Panjab University , Sector-14, Chandiagarh-160014, India.
authors.forename: R C
authors.initials: RC
authors.lastname: Sobti
keywords: Solid lipid nanoparticles; adipogenesis; mesenchymal stem cells; scaffold