title: Differential expression of miRNA in rat myocardial tissues under psychological and physical stress.
abstract: In the current study, microRNA (miRNA) microarrays were used to detect differentially expressed miRNAs in the myocardial tissues of rat models under stress, to screen target miRNA candidates for miRNA therapy of stress-induced myocardial injury. Rats were bound and suspended in order to induce acute stress (AS) and chronic stress (CS) models. miRNA microarrays were used to detect differentially expressed miRNA in the myocardial tissues of the stressed and control groups. In comparison to the normal control, there were 68 differentially expressed miRNAs in the AS model, of which 32 were upregulated and 36 were downregulated. There were 55 differentially expressed miRNAs in the CS model, of which 20 were upregulated and 35 were downregulated. Of the 123 miRNAs, 15 miRNAs were differentially expressed between the AS and CS groups, of which four were significantly upregulated (rno-miR-296, rno-miR-141, rno-miR-382 and rno-miR-219-5p) and 11 were downregulated (significantly downregulated, rno-miR-135a and rno-miR-466b). The stress of being bound and suspended induces myocardial injury in the rats. Myocardial injury may cause the differential expression of certain miRNAs. Psychological stress may lead to the significant upregulation of rno-miR-296, rno-miR-141, rno-miR-382 and rno-miR-219-5p in addition to the significant downregulation of miR-135a and miR-466b.
authors.affiliation: Department of Cardiology, The Second Hospital of Jilin University, Changchun, Jilin 130041, P.R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Cui
authors.affiliation: Institute of Genetics and Cytology, School of Life Sciences, Northeast Normal University, Changchun, Jilin 130024, P.R. China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Bai
authors.affiliation: Department of Endoscopy, The Second Hospital of Jilin University, Changchun, Jilin 130041, P.R. China.
authors.forename: Xiao Dong
authors.initials: XD
authors.lastname: Wang
authors.affiliation: Department of Cardiology, The Second Hospital of Jilin University, Changchun, Jilin 130041, P.R. China.
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.affiliation: Department of Cardiology, The Second Hospital of Jilin University, Changchun, Jilin 130041, P.R. China.
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Department of Cardiology, The Second Hospital of Jilin University, Changchun, Jilin 130041, P.R. China ; Department of Cardiology, The General Hospital of CNPC in Jilin, Jilin, Jilin 132022, P.R. China.
authors.forename: Li Shuang
authors.initials: LS
authors.lastname: Wang
keywords: microRNAs; microarray; myocardium; rat; stress