title: Identification of candidate genes for Fusarium yellows resistance in Chinese cabbage by differential expression analysis.
abstract: Fusarium yellows caused by Fusarium oxysporum f. sp. conglutinans is an important disease of Brassica worldwide. To identify a resistance (R) gene against Fusarium yellows in Chinese cabbage (Brassica rapa var. pekinensis), we analyzed differential expression at the whole genome level between resistant and susceptible inbred lines using RNA sequencing. Four hundred and eighteen genes were significantly differentially expressed, and these were enriched for genes involved in response to stress or stimulus. Seven dominant DNA markers at putative R-genes were identified. Presence and absence of the sequence of the putative R-genes, Bra012688 and Bra012689, correlated with the resistance of six inbred lines and susceptibility of four inbred lines, respectively. In F(2) populations derived from crosses between resistant and susceptible inbred lines, presence of Bra012688 and Bra012689 cosegregated with resistance, suggesting that Bra012688 and Bra012689 are good candidates for fusarium yellows resistance in Chinese cabbage.
authors.affiliation: Graduate School of Science and Technology, Niigata University, Ikarashi-ninocho, Niigata, 950-2181, Japan, motoki829nagano@gmail.com.
authors.forename: Motoki
authors.initials: M
authors.lastname: Shimizu
authors.forename: Ryo
authors.initials: R
authors.lastname: Fujimoto
authors.forename: Hua
authors.initials: H
authors.lastname: Ying
authors.forename: Zi-jing
authors.initials: ZJ
authors.lastname: Pu
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Ebe
authors.forename: Takahiro
authors.initials: T
authors.lastname: Kawanabe
authors.forename: Natsumi
authors.initials: N
authors.lastname: Saeki
authors.forename: Jennifer M
authors.initials: JM
authors.lastname: Taylor
authors.forename: Makoto
authors.initials: M
authors.lastname: Kaji
authors.forename: Elizabeth S
authors.initials: ES
authors.lastname: Dennis
authors.forename: Keiichi
authors.initials: K
authors.lastname: Okazaki
mesh_terms: D000595:Amino Acid Sequence; D029741:Brassica rapa / Q000235:genetics / Q000378:metabolism*; D005670:Fusarium / Q000737:chemistry*; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D008040:Genetic Linkage; D020022:Genetic Predisposition to Disease; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000235:genetics* / Q000276:immunology* / Q000382:microbiology; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D059467:Transcriptome
chemical_list: D018749:RNA, Plant