title: Regenerative defect in vastus lateralis muscle of patients with chronic obstructive pulmonary disease.
abstract: BACKGROUND
  Impaired skeletal muscle regeneration could contribute to the progression of muscle atrophy in patients with chronic obstructive pulmonary disease (COPD).
  
  
  METHODS
  Satellite cells and myogenesis-related proteins were compared between healthy subjects and patients with COPD, with or without muscle atrophy. Satellite cells were isolated and cultured to assess their proliferative and differentiation aptitudes.
  
  
  RESULTS
  Although satellite cell numbers in muscle samples were similar between groups, the proportion of muscle fibers with central nuclei was increased in COPD. In muscle homogenates, increased expression of MyoD and decreased expression of myogenin and MRF4 were observed in COPD. In cultured satellite cells of patients with COPD, increased protein content was observed for Pax7, Myf5 (proliferation phase) and myogenin (differentiation phase) while myosin heavy chain protein content was significantly lower during differentiation.
  
  
  CONCLUSIONS
  In COPD, the number of central nuclei was increased in muscle fibers suggesting a greater number of attempts to regenerate muscle tissue than in healthy subjects. Myogenesis signaling was also altered in muscle homogenates in patients with COPD and there was a profound reduction in the differentiation potential in this population as indicated by a reduced ability to incorporate myosin heavy chain into newly formed myotubes. Collectively, these results indicate that skeletal muscle regenerative capacity termination is impaired in COPD and could contribute to the progression of muscle atrophy progression in this population.
authors.forename: Marie-Eve
authors.initials: ME
authors.lastname: Thériault
authors.forename: Marie-Ève
authors.initials: MÈ
authors.lastname: Paré
authors.forename: Bruno B
authors.initials: BB
authors.lastname: Lemire
authors.forename: François
authors.initials: F
authors.lastname: Maltais
authors.affiliation: Centre de recherche Institut universitaire de cardiologie et de pneumologie de Québec, Université Laval, Québec, QC G1V 4G5, Canada. Richard.Debigare@rea.ulaval.ca.
authors.forename: Richard
authors.initials: R
authors.lastname: Debigaré
mesh_terms: D000368:Aged; D002478:Cells, Cultured; D006801:Humans; D008297:Male; D008875:Middle Aged; D009133:Muscular Atrophy / Q000175:diagnosis* / Q000503:physiopathology*; D029424:Pulmonary Disease, Chronic Obstructive / Q000175:diagnosis* / Q000503:physiopathology*; D052097:Quadriceps Muscle / Q000502:physiology / Q000503:physiopathology*; D012038:Regeneration / Q000502:physiology*