title: Cytoprotective effects of 6'-O-galloylpaeoniflorin against ultraviolet B radiation-induced cell damage in human keratinocytes.
abstract: The cytoprotective effects of 6'-O-galloylpaeoniflorin against injury and death of human HaCaT keratinocytes resulting from ultraviolet B radiation were investigated. 6'-O-galloylpaeoniflorin exhibited the capacity to scavenge intracellular reactive oxygen species (ROS) generated by ultraviolet B radiation. 6'-O-galloylpaeoniflorin also attenuated ultraviolet B-induced oxidative macromolecular damage to DNA, lipids, and proteins, decreasing the number of DNA strand breaks, the level of 8-isoprostane (a biomarker of lipid peroxidation), and the level of protein carbonylation. Moreover, 6'-O-galloylpaeoniflorin rescued HaCaT cells from ultraviolet induced cell death, by downregulating the mitochondrial apoptotic pathway. Taken together, these results indicate that 6'-O-galloylpaeoniflorin has the potential to be developed as a medical agent against ROS-mediated skin diseases.
authors.affiliation: School of Medicine and Institute for Nuclear Science and Technology, Jeju National University, Jeju, 690-756, Korea.
authors.forename: Cheng Wen
authors.initials: CW
authors.lastname: Yao
authors.forename: Mei Jing
authors.initials: MJ
authors.lastname: Piao
authors.forename: Ki Cheon
authors.initials: KC
authors.lastname: Kim
authors.forename: Jian
authors.initials: J
authors.lastname: Zheng
authors.forename: Ji Won
authors.initials: JW
authors.lastname: Cha
authors.forename: Chang Lim
authors.initials: CL
authors.lastname: Hyun
authors.forename: Sun Jin
authors.initials: SJ
authors.lastname: Boo
authors.forename: Soo Young
authors.initials: SY
authors.lastname: Na
authors.forename: Suk Ju
authors.initials: SJ
authors.lastname: Cho
authors.forename: Jin Won
authors.initials: JW
authors.lastname: Hyun
mesh_terms: D051033:Apoptosis Inducing Factor / Q000378:metabolism; D015153:Blotting, Western; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000494:pharmacology*; D020552:Comet Assay; D019610:Cytoprotection / Q000187:drug effects*; D004249:DNA Damage / Q000187:drug effects; D005960:Glucosides / Q000494:pharmacology*; D006801:Humans; D020287:In Situ Nick-End Labeling; D015603:Keratinocytes / Q000187:drug effects* / Q000473:pathology / Q000528:radiation effects; D015227:Lipid Peroxidation / Q000187:drug effects; D039821:Monoterpenes / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012871:Skin Diseases / Q000188:drug therapy; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D051033:Apoptosis Inducing Factor; D019086:Bridged Bicyclo Compounds, Heterocyclic; D005960:Glucosides; D039821:Monoterpenes; D017382:Reactive Oxygen Species; C119233:galloylpaeoniflorin