title: HIV-1 infection impairs regulatory T-cell suppressive capacity on a per-cell basis.
abstract: The impact of CD4+ regulatory T cells (Tregs) on human immunodeficiency virus type 1 (HIV-1) pathogenesis remains incompletely understood. Although it has been shown that Tregs can be infected with HIV-1, the consequences of infection on a per-cell basis are still unknown. In vitro HIV-GFP infected and noninfected Tregs were isolated by flow-based cell-sorting to investigate Treg suppressive capacity and gene expression profiles. Our data show that HIV-1-infected Tregs were significantly less suppressive than noninfected Tregs and demonstrated down-regulation of genes critical to Treg function. This impaired function may have detrimental consequences for the control of generalized immune activation and accelerate HIV disease progression.
authors.affiliation: Ragon Institute of MGH, MIT and Harvard.
authors.forename: Mathieu
authors.initials: M
authors.lastname: Angin
authors.affiliation: Ragon Institute of MGH, MIT and Harvard.
authors.forename: Siddhartha
authors.initials: S
authors.lastname: Sharma
authors.affiliation: Ragon Institute of MGH, MIT and Harvard.
authors.forename: Melanie
authors.initials: M
authors.lastname: King
authors.affiliation: The Center for Immunology and Inflammatory Diseases.
authors.forename: Thomas T
authors.initials: TT
authors.lastname: Murooka
authors.affiliation: Ragon Institute of MGH, MIT and Harvard.
authors.forename: Musie
authors.initials: M
authors.lastname: Ghebremichael
authors.affiliation: The Center for Immunology and Inflammatory Diseases.
authors.forename: Thorsten R
authors.initials: TR
authors.lastname: Mempel
authors.affiliation: Ragon Institute of MGH, MIT and Harvard.
authors.forename: Bruce D
authors.initials: BD
authors.lastname: Walker
authors.affiliation: BIDMC Genomics and Proteomics Center, Dana Farber/Harvard Cancer Center.
authors.forename: Manoj K
authors.initials: MK
authors.lastname: Bhasin
authors.affiliation: Ragon Institute of MGH, MIT and Harvard Massachusetts General Hospital, Division of Infectious Diseases, Boston, Massachusetts Department of Medicine I, University Hospital Hamburg-Eppendorf German Center for Infection Research (DZIF), partner site Hamburg/Lübeck/Borstel, Germany.
authors.forename: Marylyn M
authors.initials: MM
authors.lastname: Addo
mesh_terms: D015536:Down-Regulation; D005434:Flow Cytometry; D020869:Gene Expression Profiling; D015658:HIV Infections / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D007111:Immunity, Cellular; D066298:In Vitro Techniques; D050378:T-Lymphocytes, Regulatory / Q000276:immunology* / Q000502:physiology / Q000821:virology
keywords: HIV; Tregs; gene expression; immune activation; regulatory T cells