title: Identification of clinical parameters associated with serum oxidative stress in patients with rheumatoid arthritis.
abstract: OBJECTIVE
  Reactive oxygen species (ROS) are considered to be involved in the pathobiology of rheumatoid arthritis (RA); however, their association with disease activity has not been elucidated. In this study, we measured reactive oxygen metabolites (ROM) in patients with RA using a new Free Radical Analytical System and determined clinical parameters associated with ROM.
  
  
  METHODS
  One hundred and fifty-two patients with RA and 80 patients with diabetes mellitus (DM) were included in this observational study. To measure ROM, the d-ROM test was performed on blood samples drawn from all subjects. The correlation between ROM and biomarkers, disease activity, doses of methotrexate (MTX), and prednisolone (PSL) were investigated.
  
  
  RESULTS
  There were significant, positive correlations between ROM and CRP, matrix metalloproteinase 3 (MMP3), Disease Activity Score 28-erythrocyte sedimentation rate (DAS28-ESR), Clinical Disease Activity Index (CDAI), and the Simplified Disease Activity Index (SDAI). Multiple regression analysis revealed that CRP and DAS28-ESR were correlated with ROM.
  
  
  CONCLUSIONS
  The serum level of ROM was associated with CRP and DAS28-ESR, suggesting that ROM, in conjunction with CRP and MMP3, may be able to be used as a new biological disease marker to evaluate the disease activity of RA.
authors.affiliation: Department of Orthopedics, Toho University Sakura Medical Center , Sakura, Chiba , Japan.
authors.forename: Arata
authors.initials: A
authors.lastname: Nakajima
authors.forename: Yasuchika
authors.initials: Y
authors.lastname: Aoki
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Shibata
authors.forename: Masato
authors.initials: M
authors.lastname: Sonobe
authors.forename: Fumiaki
authors.initials: F
authors.lastname: Terajima
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Takahashi
authors.forename: Masahiko
authors.initials: M
authors.lastname: Saito
authors.forename: Shinji
authors.initials: S
authors.lastname: Taniguchi
authors.forename: Manabu
authors.initials: M
authors.lastname: Yamada
authors.forename: Koichi
authors.initials: K
authors.lastname: Nakagawa
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018501:Antirheumatic Agents / Q000627:therapeutic use; D001172:Arthritis, Rheumatoid / Q000097:blood* / Q000175:diagnosis / Q000188:drug therapy; D015415:Biomarkers / Q000097:blood; D003920:Diabetes Mellitus / Q000097:blood; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000502:physiology*; D017382:Reactive Oxygen Species / Q000097:blood*; D012720:Severity of Illness Index; D055815:Young Adult
chemical_list: D018501:Antirheumatic Agents; D015415:Biomarkers; D017382:Reactive Oxygen Species
keywords: Disease activity; Oxidative stress; Reactive oxygen metabolites; Rheumatoid arthritis