title: Structure of the yeast mitochondrial large ribosomal subunit.
abstract: Mitochondria have specialized ribosomes that have diverged from their bacterial and cytoplasmic counterparts. We have solved the structure of the yeast mitoribosomal large subunit using single-particle cryo-electron microscopy. The resolution of 3.2 angstroms enabled a nearly complete atomic model to be built de novo and refined, including 39 proteins, 13 of which are unique to mitochondria, as well as expansion segments of mitoribosomal RNA. The structure reveals a new exit tunnel path and architecture, unique elements of the E site, and a putative membrane docking site.
authors.affiliation: MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, United Kingdom.
authors.forename: Alexey
authors.initials: A
authors.lastname: Amunts
authors.affiliation: MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, United Kingdom.
authors.forename: Alan
authors.initials: A
authors.lastname: Brown
authors.affiliation: MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, United Kingdom.
authors.forename: Xiao-Chen
authors.initials: XC
authors.lastname: Bai
authors.affiliation: MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, United Kingdom.
authors.forename: Jose L
authors.initials: JL
authors.lastname: Llácer
authors.affiliation: MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, United Kingdom.
authors.forename: Tanweer
authors.initials: T
authors.lastname: Hussain
authors.affiliation: MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, United Kingdom.
authors.forename: Paul
authors.initials: P
authors.lastname: Emsley
authors.affiliation: MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, United Kingdom.
authors.forename: Fei
authors.initials: F
authors.lastname: Long
authors.affiliation: MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, United Kingdom.
authors.forename: Garib
authors.initials: G
authors.lastname: Murshudov
authors.affiliation: MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, United Kingdom.
authors.forename: Sjors H W
authors.initials: SHW
authors.lastname: Scheres
authors.affiliation: MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, United Kingdom.
authors.forename: V
authors.initials: V
authors.lastname: Ramakrishnan
mesh_terms: D020285:Cryoelectron Microscopy; D008928:Mitochondria / Q000648:ultrastructure*; D024101:Mitochondrial Proteins / Q000737:chemistry; D009690:Nucleic Acid Conformation; D012335:RNA, Ribosomal / Q000737:chemistry; D012343:RNA, Transfer / Q000737:chemistry; D054683:Ribosome Subunits, Large, Eukaryotic / Q000737:chemistry / Q000648:ultrastructure*; D012441:Saccharomyces cerevisiae / Q000648:ultrastructure*
chemical_list: D024101:Mitochondrial Proteins; D012335:RNA, Ribosomal; D012343:RNA, Transfer