title: High expression of long non-coding RNA H19 is required for efficient tumorigenesis induced by Bcr-Abl oncogene.
abstract: Dysregulation of non-coding RNA H19 has been observed in various tumors. However, it remains unknown whether H19 is involved in Bcr-Abl-induced leukemia. Here, we demonstrate a critical requirement for H19 in Bcr-Abl-mediated tumorigenesis. H19 was highly expressed in Bcr-Abl-transformed cell lines and primary cells derived from patients in a Bcr-Abl kinase-dependent manner. Silencing H19 expression sensitized leukemic cells to undergo imatinib-induced apoptosis and inhibited Bcr-Abl-induced tumor growth. Furthermore, H19 was shown to be regulated by c-Myc in Bcr-Abl-expressing cells. These results reveal an important role H19 plays in Bcr-Abl-mediated transformation and provide novel insights into complex mechanisms underlying Bcr-Abl-induced cancers.
authors.affiliation: CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences (CAS), Beijing 100101, China.
authors.forename: Guijie
authors.initials: G
authors.lastname: Guo
authors.affiliation: CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences (CAS), Beijing 100101, China.
authors.forename: Qingzheng
authors.initials: Q
authors.lastname: Kang
authors.affiliation: College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors.forename: Qinghuang
authors.initials: Q
authors.lastname: Chen
authors.affiliation: College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors.forename: Zhilong
authors.initials: Z
authors.lastname: Chen
authors.affiliation: CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences (CAS), Beijing 100101, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.affiliation: Center of Oncology and Hematology, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510230, China.
authors.forename: Li
authors.initials: L
authors.lastname: Tan
authors.affiliation: CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences (CAS), Beijing 100101, China; College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China. Electronic address: chenjl@im.ac.cn.
authors.forename: Ji-Long
authors.initials: JL
authors.lastname: Chen
mesh_terms: D000818:Animals; D002470:Cell Survival; D002471:Cell Transformation, Neoplastic / Q000235:genetics* / Q000378:metabolism; D005260:Female; D016044:Fusion Proteins, bcr-abl / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D015973:Gene Expression Regulation, Leukemic; D006801:Humans; D019169:Jurkat Cells; D020014:K562 Cells; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D016271:Proto-Oncogene Proteins c-myc / Q000378:metabolism; D062085:RNA, Long Noncoding / Q000235:genetics* / Q000378:metabolism
chemical_list: C094335:H19 long non-coding RNA; C489427:MYC protein, human; D016271:Proto-Oncogene Proteins c-myc; D062085:RNA, Long Noncoding; D016044:Fusion Proteins, bcr-abl
keywords: Bcr-Abl; H19; Long non-coding RNA; Tumorigenesis; c-Myc