title: Eco-friendly methodology for efficient synthesis and scale-up of 2-ethylhexyl-p-methoxycinnamate using Rhizopus oryzae lipase and its biological evaluation.
abstract: Lipase-mediated synthesis of phenolic acid esters is a green and economical alternative to current chemical methods. Octyl methoxycinnamate, an important UVB-absorbing compound, was synthesized by the esterification of p-methoxycinnamic acid with 2-ethyl hexanol using Rhizopus oryzae lipase. A molar ratio of 1:2 of p-methoxycinnamic acid and 2-ethyl hexanol was found to give an optimum yield using cyclo-octane (50 ml) as reaction solvent, at a temperature of 45 °C, and 750 U of lipase, resulting in a yield of 91.3 % in 96 h. This reaction was successfully scaled up to 400-ml reaction size where 88.6 %bioconversion was achieved. The synthesized compound was found to have superior antioxidant activity as compared to ascorbic acid. The synthesized compound also exhibited good antimicrobial activity against Escherichia coli, Klebsiella pneumonia, Salmonella typhi, Staphylococcus aures, Candida albicans (yeast), Aspergillus niger, Alternaria solani, and Fussarium oxysporum by well diffusion method in terms of zone of inhibitions (in mm).
authors.affiliation: Department of Microbiology, University of Delhi South Campus, New Delhi, 110021, India.
authors.forename: Vinod
authors.initials: V
authors.lastname: Kumar
authors.forename: Firdaus
authors.initials: F
authors.lastname: Jahan
authors.forename: Karthikeya
authors.initials: K
authors.lastname: Kameswaran
authors.forename: Richi V
authors.initials: RV
authors.lastname: Mahajan
authors.forename: Rajendra Kumar
authors.initials: RK
authors.lastname: Saxena
mesh_terms: D000890:Anti-Infective Agents / Q000378:metabolism / Q000494:pharmacology; D060326:Chemistry Techniques, Synthetic / Q000379:methods; D002934:Cinnamates / Q000378:metabolism* / Q000494:pharmacology; D004951:Esterification; D055772:Green Chemistry Technology; D008049:Lipase / Q000378:metabolism*; D012233:Rhizopus / Q000201:enzymology*
chemical_list: D000890:Anti-Infective Agents; D002934:Cinnamates; C118580:octylmethoxycinnamate; D008049:Lipase