title: Simultaneous separation of apigenin, luteolin and rosmarinic acid from the aerial parts of the copper-tolerant plant Elsholtzia splendens.
abstract: Elsholtzia splendens is a copper-tolerant plant species which grows on copper deposits in China. The generation of a valuable E. splendens biomass on specific contaminated sites has become one of the promising phytotechnologies. The simultaneous separations of apigenin, luteolin, and rosmarinic acid yielded in the ethyl acetate extracts of the flowering aerial parts was achieved by the use of a macroporous resin, polyamide, and silicagel columns during chromatography. Chemical identification confirmed the structures based on the spectra of FTIR, NMR, and HPLC/ESI-MS. The isolated compounds of purity above 98.3% were evaluated for their in vitro cytotoxic activities against human cancer cell lines including A549 (non-small lung), A431 (skin), and Bcap37 (breast). Among these compounds, luteolin and apigenin presented the best cytotoxic activities against A549, A431, and Bcap37 cells and, therefore, both could be the valuable products for the post-harvest processing of E. splendens biomass.
authors.affiliation: Key Laboratory of Environmental Remediation and Ecological Health, Ministry of Education, College of Environmental and Resource Sciences, Zijingang Campus, Zhejiang University, No. 866 Yuhangtang Rd, Hangzhou, 310058, China, penghongyun@zju.edu.cn.
authors.forename: Hongyun
authors.initials: H
authors.lastname: Peng
authors.forename: Yan
authors.initials: Y
authors.lastname: Xing
authors.forename: Lingling
authors.initials: L
authors.lastname: Gao
authors.forename: Liang
authors.initials: L
authors.lastname: Zhang
authors.forename: Guolin
authors.initials: G
authors.lastname: Zhang
mesh_terms: D000085:Acetates; D047310:Apigenin / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification* / Q000633:toxicity; D001673:Biodegradation, Environmental; D045744:Cell Line, Tumor; D002681:China; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D002934:Cinnamates / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification* / Q000633:toxicity; D003300:Copper; D053630:Depsides / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification* / Q000633:toxicity; D035264:Flowers / Q000737:chemistry*; D006801:Humans; D019686:Lamiaceae / Q000737:chemistry*; D047311:Luteolin / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification* / Q000633:toxicity; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008906:Mining; D015394:Molecular Structure; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification* / Q000633:toxicity; D000091402:Prohibitins; D017550:Spectroscopy, Fourier Transform Infrared; D000097123:Rosmarinic Acid
chemical_list: D000085:Acetates; D047310:Apigenin; D002934:Cinnamates; D003300:Copper; D053630:Depsides; D047311:Luteolin; D010936:Plant Extracts; D000091402:Prohibitins; D000097123:Rosmarinic Acid; C000717331:PHB2 protein, human; C007650:ethyl acetate