title: Enhanced levels of S-linalool by metabolic engineering of the terpenoid pathway in spike lavender leaves.
abstract: Transgenic Lavandula latifolia plants overexpressing the linalool synthase (LIS) gene from Clarkia breweri, encoding the LIS enzyme that catalyzes the synthesis of linalool were generated. Most of these plants increased significantly their linalool content as compared to controls, especially in the youngest leaves, where a linalool increase up to a 1000% was observed. The phenotype of increased linalool content observed in young leaves was maintained in those T1 progenies that inherit the LIS transgene, although this phenotype was less evident in the flower essential oil. Cross-pollination of transgenic spike lavender plants allowed the generation of double transgenic plants containing the DXS (1-deoxy-d-xylulose-5-P synthase), coding for the first enzyme of the methyl-d-erythritol-4-phosphate pathway, and LIS genes. Both essential oil yield and linalool content in double DXS-LIS transgenic plants were lower than that of their parentals, which could be due to co-suppression effects linked to the structures of the constructs used.
authors.affiliation: Departamento de Biología Vegetal, Universidad de Valencia, Av. Vicent Andrés Estellés S/N, Burjasot, 46100 Valencia, Spain; ISIC/ERI de Biotecnología y Biomedicina, Universidad de Valencia, Av. Vicent Andrés Estellés S/N, Burjasot, 46100 Valencia, Spain.
authors.forename: Isabel
authors.initials: I
authors.lastname: Mendoza-Poudereux
authors.affiliation: Instituto de Biología Molecular y Celular de Plantas, Departamento Biotecnología, Universidad Politécnica de Valencia-Consejo Superior de Investigaciones Científicas, C/Ingeniero Fausto Elio, 46022 Valencia, Spain.
authors.forename: Jesús
authors.initials: J
authors.lastname: Muñoz-Bertomeu
authors.affiliation: Departamento de Biología Vegetal, Universidad de Valencia, Av. Vicent Andrés Estellés S/N, Burjasot, 46100 Valencia, Spain; ISIC/ERI de Biotecnología y Biomedicina, Universidad de Valencia, Av. Vicent Andrés Estellés S/N, Burjasot, 46100 Valencia, Spain.
authors.forename: Alicia
authors.initials: A
authors.lastname: Navarro
authors.affiliation: Departamento de Biología Vegetal, Universidad de Valencia, Av. Vicent Andrés Estellés S/N, Burjasot, 46100 Valencia, Spain; ISIC/ERI de Biotecnología y Biomedicina, Universidad de Valencia, Av. Vicent Andrés Estellés S/N, Burjasot, 46100 Valencia, Spain.
authors.forename: Isabel
authors.initials: I
authors.lastname: Arrillaga
authors.affiliation: Departamento de Biología Vegetal, Universidad de Valencia, Av. Vicent Andrés Estellés S/N, Burjasot, 46100 Valencia, Spain; ISIC/ERI de Biotecnología y Biomedicina, Universidad de Valencia, Av. Vicent Andrés Estellés S/N, Burjasot, 46100 Valencia, Spain. Electronic address: juan.segura@uv.es.
authors.forename: Juan
authors.initials: J
authors.lastname: Segura
mesh_terms: D000080462:Acyclic Monoterpenes; D031663:Clarkia / Q000201:enzymology / Q000235:genetics; D004896:Erythritol / Q000031:analogs & derivatives / Q000235:genetics / Q000378:metabolism; D006836:Hydro-Lyases / Q000096:biosynthesis / Q000235:genetics; D027523:Lavandula / Q000235:genetics / Q000378:metabolism; D039821:Monoterpenes / Q000378:metabolism*; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D013403:Sugar Phosphates / Q000235:genetics / Q000378:metabolism; D019076:Transgenes
chemical_list: C114232:2-C-methylerythritol 4-phosphate; D000080462:Acyclic Monoterpenes; D039821:Monoterpenes; D010940:Plant Proteins; D013403:Sugar Phosphates; C018584:linalool; D006836:Hydro-Lyases; C092055:S-linalool synthase; D004896:Erythritol
keywords: Essential oils; Lavandula latifolia; Linalool synthase; Metabolic engineering; Monoterpenes