title: Bioorthogonal site-specific labeling of the 5'-cap structure in eukaryotic mRNAs.
abstract: We present a chemo-enzymatic approach for site-specific labeling of 5'-capped RNAs based on bioorthogonal chemistry. A trimethylguanosine synthase was engineered to transfer a terminal azido moiety to the 5'-cap which could be further modified using strain-promoted azide-alkyne cycloaddition (SPAAC).
authors.affiliation: Institute of Biochemistry, University of Muenster, Wilhelm-Klemm-Str. 2, D-48149 Münster, Germany. a.rentmeister@uni-muenster.de.
authors.forename: Josephin Marie
authors.initials: JM
authors.lastname: Holstein
authors.forename: Daniela
authors.initials: D
authors.lastname: Schulz
authors.forename: Andrea
authors.initials: A
authors.lastname: Rentmeister
mesh_terms: D000480:Alkynes / Q000737:chemistry; D001386:Azides / Q000737:chemistry; D002232:Carbocyanines / Q000737:chemistry; D061565:Cycloaddition Reaction; D005872:Giardia / Q000201:enzymology; D008780:Methyltransferases / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000737:chemistry*; D012235:Rhodamines / Q000737:chemistry
chemical_list: D000480:Alkynes; D001386:Azides; D002232:Carbocyanines; D012333:RNA, Messenger; D012235:Rhodamines; C085321:cyanine dye 5; C022027:lissamine rhodamine B; D008780:Methyltransferases; C456271:trimethylguanosine synthase