title: Up-regulation of microRNA-138 induce radiosensitization in lung cancer cells.
abstract: Deregulation of microRNAs (miRNAs) is implicated in tumor progression. We attempt to identify the association between miR-138 and Sentrin/SUMO-specific protease 1 (SENP1) as a radiosensitization-related gene and characterize the biological function by which SENP1 was regulated by miR-138 to influence radiosensitization in lung cancer cells. In this study, we showed that miRNA-138 is reduced in both lung cancer clinical specimens and cell lines and is effective to inhibit SENP1 expression. Moreover, high levels of miR-138 are associated with lower levels of lung cancer cell proliferation and colony formation. Then, we investigated the underlying mechanisms responsible for the increase in the radiosensitivity of lung cancer cells when SENP1 is inhibited by miR-138. We further show that the increased radiosensitivity may be the result of an increased γ-H2AX expression, an increased rate of apoptosis, and changes in the cell cycle. In conclusion, our data demonstrate that the miR-138/SENP1 cascade is relative to radiosensitization in lung cancer cells and is a potential radiotherapy target.
authors.affiliation: Department of Nuclear Medicine, Affiliated Tumor Hospital of Zhengzhou University, Zhengzhou, 450008, Henan, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Yang
authors.forename: Yue
authors.initials: Y
authors.lastname: Tang
authors.forename: Wei
authors.initials: W
authors.lastname: Guo
authors.forename: Yuwen
authors.initials: Y
authors.lastname: Du
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Wang
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.forename: Wenqiao
authors.initials: W
authors.lastname: Zang
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Yin
authors.forename: Huaqi
authors.initials: H
authors.lastname: Wang
authors.forename: Heying
authors.initials: H
authors.lastname: Chu
authors.forename: Guojun
authors.initials: G
authors.lastname: Zhang
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Zhao
mesh_terms: D017209:Apoptosis / Q000235:genetics; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000528:radiation effects; D003546:Cysteine Endopeptidases; D010450:Endopeptidases / Q000096:biosynthesis* / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology / Q000532:radiotherapy*; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D011836:Radiation Tolerance / Q000235:genetics
chemical_list: C546110:MIRN138 microRNA, human; D035683:MicroRNAs; D010450:Endopeptidases; C404512:SENP1 protein, human; D003546:Cysteine Endopeptidases