title: Phylogeny and virulence divergency analyses of Toxoplasma gondii isolates from China.
abstract: BACKGROUND
  Toxoplasma gondii (T. gondii) is a very successful parasite that can infect virtually all warm blooded animals with a worldwide distribution. It causes a large range of clinical manifestations in both humans and domesticated animals. In addition, marked biological differences exist among T. gondii strains in the pathogenicity and geographical distribution. Molecular epidemiology studies primarily based on restriction fragment length polymorphism (RFLP) method revealed that three main types are predominant in North America and Europe, whereas other diverse genotypes are found in other parts of the world. Microsatellite (MS) as a type of genetic marker has been widely used in many organisms. Limited MS genotyping, however, to fingerprint T. gondii isolates has been reported and little is known about the MS data of the strains predominantly prevalent in China.
  
  
  METHODS
  Genotyping of twenty-eight Chinese T. gondii isolates were performed using 15 MS markers located on 12 different chromosomes. Results were analyzed in terms of population structure by a Bayesian statistical approach. Phylogenetic analysis was obtained from a Neighbor-Net phylogenetic network. The virulence analyses of some representative isolates were determined by inoculation of mice and cell invasion assays. The gene expressions of some virulence-associated factors (VFs) were performed by quantitative real-time PCR (qRT- PCR).
  
  
  RESULTS
  Three haplogroups were clustered among the 28 isolates although minor genetic differences were found within haplogroups. The majority of strains belong to one haplogroup corresponding to the previously described Chinese 1 type (ToxoDB#9). Phylogenetic networks uncovered a limited diversity of T. gondii strains and the virulence differs in the strains sharing the same genotype. No remarkable difference, however, was noted in the tested VFs except for dense granule protein3 (GRA3), which was found to have a higher expression in low virulent TgCtwh6 (Wh6) strain than that in high virulent TgCtwh3 (Wh3) strain.
  
  
  CONCLUSIONS
  The profile of microsatellite typing data from Chinese T. gondii strains revealed a limited genetic diversity and the selected VFs and phylogenetic network analyses displayed less divergence, although the strain virulence differs in the Chinese 1 type of T. gondii predominantly prevalent in China.
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.forename: Xu-Wei
authors.initials: XW
authors.lastname: Mo
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.forename: He
authors.initials: H
authors.lastname: Chen
authors.forename: Qing-Li
authors.initials: QL
authors.lastname: Luo
authors.forename: Hui-Qin
authors.initials: HQ
authors.lastname: Wen
authors.forename: Wei
authors.initials: W
authors.lastname: Wei
authors.forename: Ai-Mei
authors.initials: AM
authors.lastname: Zhang
authors.forename: Jian
authors.initials: J
authors.lastname: Du
authors.forename: Fang-Li
authors.initials: FL
authors.lastname: Lu
authors.forename: Zhao-Rong
authors.initials: ZR
authors.lastname: Lun
authors.affiliation: Anhui Provincial Laboratories of Pathogen Biology and Zoonoses, Department of Microbiology and Parasitology, Anhui Medical University, Hefei, Anhui, China. shenjilong53@126.com.
authors.forename: Ji-Long
authors.initials: JL
authors.lastname: Shen
mesh_terms: D000818:Animals; D002415:Cats; D002681:China / Q000453:epidemiology; D014644:Genetic Variation; D051379:Mice; D018895:Microsatellite Repeats; D010802:Phylogeny; D014122:Toxoplasma / Q000235:genetics* / Q000472:pathogenicity*; D014124:Toxoplasmosis, Animal / Q000453:epidemiology / Q000469:parasitology; D014774:Virulence