title: Effect of experimental parameters in the pressurized solvent extraction of polyphenolic compounds from white grape marc.
abstract: A method based on pressurized solvent extraction (PSE) to determine main polyphenolic compounds in the grape marc obtained as a byproduct of the white winemaking process has been developed. As response variables in the optimisation process include main individual polyphenols, as well as spectrophotometric indexes. The optimised PSE procedure implies the use of 1 g of sample, without preliminary clean-up step, sea sand as dispersant, temperature of 105 °C, methanol (63%) in water as solvent, and 5 min of extraction time (2 static cycles). The performance of the proposed method has been assessed in terms of recovery (91-105%), linearity (R(2)>0.995) and precision. The applicability of the method was demonstrated by the analysis of bagasse samples collected from 12 wineries located in Galicia (NW Spain). Data of the in vitro antioxidant activities of the PSE extracts are also discussed.
authors.affiliation: Department of Analytical Chemistry, Nutrition and Food Science, Faculty of Chemistry, University of Santiago de Compostela, Avda. das Ciencias, s/n, 15782 Santiago de Compostela, Spain.
authors.forename: Marta
authors.initials: M
authors.lastname: Álvarez-Casas
authors.affiliation: Department of Analytical Chemistry, Nutrition and Food Science, Faculty of Chemistry, University of Santiago de Compostela, Avda. das Ciencias, s/n, 15782 Santiago de Compostela, Spain.
authors.forename: Carmen
authors.initials: C
authors.lastname: García-Jares
authors.affiliation: Department of Analytical Chemistry, Nutrition and Food Science, Faculty of Chemistry, University of Santiago de Compostela, Avda. das Ciencias, s/n, 15782 Santiago de Compostela, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Llompart
authors.affiliation: Department of Analytical Chemistry, Nutrition and Food Science, Faculty of Chemistry, University of Santiago de Compostela, Avda. das Ciencias, s/n, 15782 Santiago de Compostela, Spain. Electronic address: marta.lores@usc.es.
authors.forename: Marta
authors.initials: M
authors.lastname: Lores
mesh_terms: D000975:Antioxidants / Q000032:analysis; D002392:Catechin / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D005707:Gallic Acid / Q000032:analysis; D016014:Linear Models; D059808:Polyphenols / Q000032:analysis*; D012997:Solvents / Q000737:chemistry; D013030:Spain; D053719:Tandem Mass Spectrometry; D013696:Temperature; D027843:Vitis / Q000737:chemistry*; D014867:Water
chemical_list: D000975:Antioxidants; D059808:Polyphenols; D012997:Solvents; D014867:Water; D005707:Gallic Acid; D002392:Catechin
keywords: Antioxidant activity; Experimental design; Grape marc; Polyphenols; Pressurized solvent extraction