title: [Fragmentation behaviors of taxanes drugs using electrospray ionization with quadrupole time-of-flight mass spectrometry].
abstract: The fragmentation pathways of two taxanes drugs have been studied in positive ion mode by Q-TOF with the advantages of high mass accuracy and high resolution analysis. The [M+H] + ions were observed by ESI-MS, from which the molecular weights were obtained. Using the protonated pseudo-molecular ions [M+H]+ as internal reference compounds, the accurate mass and element composition of the fragment ions were determined. The collision induced dissociation (CID) data of the [M+H] ions provided fragmentation pathways of related compounds. Results showed that the major cleavage pathways of paclitaxel and docetaxel were the same that the cleavage of C-O bond between the side chain and taxol skeleton easily occurred, then stripping of the functional groups on the parent ring. Some common fragments were formed, such as m/z 105.033 7, 291.137 3, 309.148 5, 327.159 7, 387.181 2 and 509.217 4, which would provide a basis for future qualitative and quantitative analysis of taxanes in vitro and in vivo.
authors.forename: Wen-Xiao
authors.initials: WX
authors.lastname: Ma
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.forename: Ting-Ting
authors.initials: TT
authors.lastname: Wang
authors.forename: Yan
authors.initials: Y
authors.lastname: Yang
authors.forename: Jing-Kai
authors.initials: JK
authors.lastname: Gu
mesh_terms: D000077143:Docetaxel; D017239:Paclitaxel / Q000737:chemistry*; D010446:Peptide Fragments / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D043823:Taxoids / Q000737:chemistry*
chemical_list: D010446:Peptide Fragments; D043823:Taxoids; D000077143:Docetaxel; D017239:Paclitaxel