title: Allergic airway inflammation by nasal inoculation of particulate matter (PM2.5) in NC/Nga mice.
abstract: To evaluate the effect of airborne particulate matter 2.5 (PM2.5) in winter on airway inflammation, water-soluble supernatant (Sup) and water-insoluble precipitate (Pre) in PM2.5 were inoculated in NC/Nga mice with high sensitivity to mite allergens. Sup with aluminum oxide was injected intraperitoneally for sensitization. Five days later, Sup, Pre or both Sup and Pre were inoculated via the nasal route five times for more sensitization and a challenge inoculation on the 11th day in NC/Nga mice. On the 12th day, mice were examined for airway hyperresponsiveness (AHR), BALF cell count and IL-1β concentration, mRNA expression of Th1 and Th2 cytokines, chemokines such as eotaxin 1 and eotaxin 2, inflammasomal complex molecules such as IL-1β, caspase 1 and the nucleotide-binding domain and leucine-rich repeat protein 3 (NLRP3) in lung tissue as well as histopathology. The synergistic effect of Sup and Pre was observed in terms of increases in AHR, BALF cells, the mRNA expression of IL-13, eotaxin1 and IL-1β, and the IL-1β concentration in BALF. Intracellular deposits of insoluble particulates were observed in macrophages around inflammatory granulation of the mouse group treated with Sup and Pre. These results suggest that PM2.5 can induce airway hyperresponsiveness in mice with genetically high sensitivity to mite allergens by an inflammasome-associated mechanism and synergistic action of insoluble particulates and soluble components.
authors.affiliation: Department of Public Health, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
authors.forename: Keiki
authors.initials: K
authors.lastname: Ogino
authors.affiliation: Department of Public Health, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
authors.forename: Ran
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Department of Public Health, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi, Japan.
authors.forename: Hidekazu
authors.initials: H
authors.lastname: Takahashi
authors.affiliation: Department of Public Health, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
authors.forename: Kei
authors.initials: K
authors.lastname: Takemoto
authors.affiliation: Department of Public Health, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Kubo
authors.affiliation: Third Institute of New Drug Discovery, Otsuka Pharmaceutical Co., Ltd., 463-10 Kagasuno, Kawauchi-cho, Tokushima, Japan.
authors.forename: Ikuo
authors.initials: I
authors.lastname: Murakami
authors.affiliation: Department of Public Health, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
authors.forename: Da-Hong
authors.initials: DH
authors.lastname: Wang
authors.affiliation: Department of Molecular Anatomy, Oita University Faculty of Medicine, 1-1, Idaigaoka, Hasama-machi, Yufu, Oita, Japan.
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Fujikura
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000281:Administration, Intranasal; D000818:Animals; D016535:Bronchial Hyperreactivity / Q000150:complications / Q000473:pathology; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D002352:Carrier Proteins / Q000378:metabolism; D020170:Caspase 1 / Q000378:metabolism; D002452:Cell Count; D018925:Chemokines / Q000235:genetics / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D006967:Hypersensitivity / Q000150:complications* / Q000473:pathology; D007249:Inflammation / Q000139:chemically induced / Q000150:complications* / Q000473:pathology; D053583:Interleukin-1beta / Q000378:metabolism; D008168:Lung / Q000187:drug effects / Q000473:pathology*; D008297:Male; D008670:Metals / Q000032:analysis; D051379:Mice; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; D009930:Organic Chemicals / Q000032:analysis; D052638:Particulate Matter / Q000008:administration & dosage* / Q000633:toxicity*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; D018925:Chemokines; D053583:Interleukin-1beta; D008670:Metals; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; C492716:Nlrp3 protein, mouse; D009930:Organic Chemicals; D052638:Particulate Matter; D012333:RNA, Messenger; D020170:Caspase 1; D000109:Acetylcholine