title: 5'-end labeling of RNA with [γ-32P]ATP and T4 polynucleotide kinase.
abstract: This protocol uses T4 polynucleotide kinase to catalyze the transfer of a radiolabeled, terminal (γ) phosphate of ATP to the 5'-hydroxyl terminus of a DNA or RNA molecule. The reaction is very efficient and hence is used as a general method for phosphorylating polynucleotides or oligonucleotides.
authors.forename: Donald C
authors.initials: DC
authors.lastname: Rio
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D017122:Bacteriophage T4 / Q000201:enzymology; D004247:DNA / Q000378:metabolism; D010761:Phosphorus Radioisotopes / Q000378:metabolism*; D011116:Polynucleotide 5'-Hydroxyl-Kinase / Q000378:metabolism*; D012313:RNA / Q000378:metabolism*; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D010761:Phosphorus Radioisotopes; D012313:RNA; D000255:Adenosine Triphosphate; D004247:DNA; D011116:Polynucleotide 5'-Hydroxyl-Kinase