title: A surface-stress-based microcantilever aptasensor.
abstract: Biosensors based on microcantilevers convert biological recognition events into measurable mechanical displacements. They offer advantages such as small size, low sample volume, label-free detection, ease of integration, high-throughput analysis, and low development cost. The design and development of a microcantilever-based aptasensor employing SU-8 polymer as the fabrication material is presented in this paper. Aptamers are employed as bioreceptor elements because they exhibit superior specificity compared to antibodies due to their small size and physicochemical stability. To immobilise thrombin DNA aptamer on the bare SU-8 surface of the aptasensor, a combined plasma mode treatment method is implemented which modifies the surface of the aptasensor. Label-free detection of thrombin molecules using the fabricated aptasensor is successfully demonstrated. The measured deflection is one order of magnitude higher than that of a silicon nitride microcantilever biosensor. The developed aptasensor also demonstrates high specificity.
authors.forename: Yang Choon
authors.initials: YC
authors.lastname: Lim
authors.forename: Abbas Z
authors.initials: AZ
authors.lastname: Kouzani
authors.forename: Wei
authors.initials: W
authors.lastname: Duan
authors.forename: Xiujuan J
authors.initials: XJ
authors.lastname: Dai
authors.forename: Akif
authors.initials: A
authors.lastname: Kaynak
authors.forename: Douglas
authors.initials: D
authors.lastname: Mair
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D061925:Immobilized Nucleic Acids / Q000737:chemistry*; D008856:Microscopy, Fluorescence; D012680:Sensitivity and Specificity; D013314:Stress, Mechanical
chemical_list: D052157:Aptamers, Nucleotide; D061925:Immobilized Nucleic Acids; C081676:thrombin aptamer