title: Macromolecular uptake of alkyl-chain-modified guanidinoglycoside molecular transporters.
abstract: Guanidinoglycosides, a family of cellular transporters capable of delivering high Mw biopolymers, have previously been shown to display high selectivity for cell-surface heparan sulfate proteoglycans and promote their clustering. Herein, the internalization mechanism of amphiphilic guanidinoglycoside derivatives was investigated by cell-surface FRET analysis. Unexpectedly, although the heparan sulfate selectivity is maintained, the cellular uptake of these derivatives does not appear to involve clustering of the proteoglycans on the cell surface. This suggests a distinct uptake mechanism when compared to the parent guanidinoglycoside-based carriers.
authors.forename: Makoto
authors.initials: M
authors.lastname: Inoue
authors.forename: Ezequiel
authors.initials: E
authors.lastname: Wexselblatt
authors.forename: Jeffrey D
authors.initials: JD
authors.lastname: Esko
authors.forename: Yitzhak
authors.initials: Y
authors.lastname: Tor
mesh_terms: D000818:Animals; D001692:Biological Transport; D016466:CHO Cells; D002462:Cell Membrane / Q000378:metabolism; D003412:Cricetulus; D004705:Endocytosis; D006027:Glycosides / Q000737:chemistry / Q000378:metabolism*; D019791:Guanidine / Q000031:analogs & derivatives* / Q000378:metabolism; D019812:Heparan Sulfate Proteoglycans / Q000378:metabolism; D006497:Heparan Sulfate / Q000378:metabolism*; D011509:Proteoglycans / Q000378:metabolism
chemical_list: D006027:Glycosides; D019791:Guanidine; D019812:Heparan Sulfate Proteoglycans; D006497:Heparan Sulfate; D011509:Proteoglycans