title: Tryptophan as the fingerprint for distinguishing aggressiveness among breast cancer cell lines using native fluorescence spectroscopy.
abstract: Tryptophan is investigated as the key native marker in cells to determine the level of metastasis competence in breast cell lines using native fluorescence spectroscopy. The ratio of fluorescence intensity at 340 nm to intensity at 460 nm is associated with aggressiveness of the cancer cells. We found that the fluorescence of aggressive breast cancer cell has a much higher contribution from tryptophan compared with that from the normal cells and nonaggressive breast cancer cell.
authors.affiliation: City College of the City University of New York, Department of Biomedical Engineering, 160 Convent Avenue, New York, New York 10031.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.affiliation: City College of the City University of New York, Department of Physics, Institute for Ultrafast Spectroscopy and Lasers, 160 Convent Avenue, New York, New York 10031.
authors.forename: Yang
authors.initials: Y
authors.lastname: Pu
authors.affiliation: Washington University in St. Louis, Department of Radiology, School of Medicine, 4525 Scott Avenue, St. Louis, Missouri 63110.
authors.forename: Jianpeng
authors.initials: J
authors.lastname: Xue
authors.affiliation: City College of the City University of New York, Department of Physics, Institute for Ultrafast Spectroscopy and Lasers, 160 Convent Avenue, New York, New York 10031dUniversidade de São Paulo, Instituto de Física de São Carlos, São Carlos 13566-590, Brazi.
authors.forename: Sebastião
authors.initials: S
authors.lastname: Pratavieira
authors.affiliation: Washington University in St. Louis, Department of Radiology, School of Medicine, 4525 Scott Avenue, St. Louis, Missouri 63110.
authors.forename: Baogang
authors.initials: B
authors.lastname: Xu
authors.affiliation: Washington University in St. Louis, Department of Radiology, School of Medicine, 4525 Scott Avenue, St. Louis, Missouri 63110.
authors.forename: Samuel
authors.initials: S
authors.lastname: Achilefu
authors.affiliation: City College of the City University of New York, Department of Physics, Institute for Ultrafast Spectroscopy and Lasers, 160 Convent Avenue, New York, New York 10031.
authors.forename: Robert R
authors.initials: RR
authors.lastname: Alfano
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis*; D001943:Breast Neoplasms / Q000737:chemistry* / Q000145:classification*; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D060388:Support Vector Machine; D014364:Tryptophan / Q000032:analysis*
chemical_list: D014408:Biomarkers, Tumor; D014364:Tryptophan