title: Metallic ion content and damage to the DNA in oral mucosa cells patients treated dental implants.
abstract: The aim of this study was to assess the potential genotoxicity of dental implants, evaluating biomarkers of DNA damage (micronuclei and/or nuclear buds), cytokinetic defects (binucleated cells) and the presence of trace metals in gingival cells of patients with implants, comparing these with a control group. A total of 60 healthy adults (30 patients with dental implants and 30 control patients without) were included in the study. Medical and dental histories were made for each including life-style factors. Genotoxicity effects were assessed by micronucleus assays in the gingival epithelial cells of each patient; 1,000 epithelial cells were analyzed, evaluating the frequency of micronucleated cells and other nuclear anomalies. The concentration of metals (Al(27), Ag(107), Co (59), Cr (52), Cu(63), Fe(56), Sn(118), Mn(55), Mo(92), Ni(60), Pb(208), Ti(47)) were assayed by means of coupled plasma-mass spectrophotometry (ICP-MS). The frequency of micronuclei in the patient group with implants was higher than in the control group but without statistically significant differences (P > 0.05). Similar results were found for binucleated cells and nuclear buds (P > 0.05). For metals assayed by ICP-MS, significant differences were found for Ti(47) (P ≤ 0.045). Univariate analysis identified a significant association between the presence of micronuclei and age. Dental implants do not induce DNA damage in gingival cells, the slight effects observed cannot be indicated as biologically relevant.
authors.affiliation: Oral Medicine Department, Faculty of Medicine and Dentistry, Ageing Research Institute, University of Murcia, Murcia, Spain, majornet@um.es.
authors.forename: Pía
authors.initials: P
authors.lastname: López-Jornet
authors.forename: Francisco Parra
authors.initials: FP
authors.lastname: Perrez
authors.forename: José Luis
authors.initials: JL
authors.lastname: Calvo-Guirado
authors.forename: Irene
authors.initials: I
authors.lastname: Ros-Llor
authors.forename: Irene
authors.initials: I
authors.lastname: LLor-Ros
authors.forename: Piedad
authors.initials: P
authors.lastname: Ramírez-Fernández
mesh_terms: D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D002467:Cell Nucleus / Q000378:metabolism; D004249:DNA Damage; D015921:Dental Implants; D004847:Epithelial Cells / Q000166:cytology; D005260:Female; D005881:Gingiva / Q000378:metabolism; D006801:Humans; D007477:Ions / Q000737:chemistry*; D008297:Male; D013058:Mass Spectrometry; D008670:Metals / Q000737:chemistry*; D015162:Micronucleus Tests; D008875:Middle Aged; D009061:Mouth Mucosa / Q000378:metabolism*
chemical_list: D015921:Dental Implants; D007477:Ions; D008670:Metals