title: Drosophila Mcm10 is required for DNA replication and differentiation in the compound eye.
abstract: Mini chromosome maintenance 10 (Mcm10) is an essential protein, which is conserved from S. cerevisiae to Drosophila and human, and is required for the initiation of DNA replication. Knockdown of Drosophila Mcm10 (dMcm10) by RNA interference in eye imaginal discs induces abnormal eye morphology (rough eye phenotype), and the number of ommatidia is decreased in adult eyes. We also observed a delay in the S phase and M phase in eye discs of dMcm10 knockdown fly lines. These results show important roles for dMcm10 in the progression of S and M phases. Furthermore, genome damage and apoptosis were induced by dMcm10 knockdown in eye imaginal discs. Surprisingly, when we used deadpan-lacZ and klingon-lacZ enhancer trap lines to monitor the photoreceptor cells in eye discs, knockdown of dMcm10 by the GMR-GAL4 driver reduced the signals of R7 photoreceptor cells. These data suggest an involvement of dMcm10 in R7 cell differentiation. This involvement appears to be independent of the apoptosis induced by dMcm10 knockdown. Together, these results suggest that dMcm10 knockdown has an effect on DNA replication and R7 cell differentiation.
authors.affiliation: Department of Applied Biology, Kyoto Institute of Technology, Kyoto, Japan; Insect Biomedical Research Center, Kyoto Institute of Technology, Kyoto, Japan.
authors.forename: Nicole
authors.initials: N
authors.lastname: Vo
authors.affiliation: Department of Applied Biology, Kyoto Institute of Technology, Kyoto, Japan; Insect Biomedical Research Center, Kyoto Institute of Technology, Kyoto, Japan.
authors.forename: Ayano
authors.initials: A
authors.lastname: Taga
authors.affiliation: Department of Applied Biology, Kyoto Institute of Technology, Kyoto, Japan; Insect Biomedical Research Center, Kyoto Institute of Technology, Kyoto, Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Inaba
authors.affiliation: Department of Applied Biology, Kyoto Institute of Technology, Kyoto, Japan; Insect Biomedical Research Center, Kyoto Institute of Technology, Kyoto, Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Yoshida
authors.affiliation: Department of Basic Medical Sciences, St Georges University of London, London, United Kingdom.
authors.forename: Sue
authors.initials: S
authors.lastname: Cotterill
authors.affiliation: Department of Applied Biology, Kyoto Institute of Technology, Kyoto, Japan; Insect Biomedical Research Center, Kyoto Institute of Technology, Kyoto, Japan.
authors.forename: Masamitsu
authors.initials: M
authors.lastname: Yamaguchi
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics / Q000378:metabolism; D017209:Apoptosis / Q000235:genetics; D002454:Cell Differentiation / Q000235:genetics*; D002455:Cell Division / Q000235:genetics; D004261:DNA Replication / Q000235:genetics*; D004330:Drosophila / Q000235:genetics; D029721:Drosophila Proteins / Q000235:genetics*; D005123:Eye / Q000378:metabolism*; D060227:Imaginal Discs / Q000378:metabolism*; D064110:Minichromosome Maintenance Proteins / Q000235:genetics*; D055495:Neurogenesis / Q000235:genetics; D010641:Phenotype; D017956:Photoreceptor Cells, Invertebrate / Q000378:metabolism; D016196:S Phase / Q000235:genetics
chemical_list: D029721:Drosophila Proteins; C489612:MCM10 protein, Drosophila; D064110:Minichromosome Maintenance Proteins