title: Antibacterial performance of nanocrystallined titania confined in mesoporous silica nanotubes.
abstract: In this paper, we study synthesis and characteristics of mesoporous silica nanotubes modified by titanium dioxide, as well as their antimicrobial properties and influence on mitochondrial activity of mouse fibroblast L929. Nanocrystalized titania is confined in mesopores of silica nanotubes and its light activated antibacterial response is revealed. The analysis of the antibacterial effect on Escherichia coli. (ATCC 25922) shows strong enhancement during irradiation with the artificial visible and ultraviolet light in respect to the commercial catalyst and control sample free from the nanomaterials. In darkness, the mesoporous silica/titania nanostructures exhibited antibacterial activity dependent on the stirring speed of the suspension containing nanomaterials. Obtained micrograph proved internalization of the sample into the microorganism trough the cell membrane. The analysis of the mitochondrial activity and amount of lactate dehydrogenase released from mouse fibroblast cells L929 in the presence of the sample were determined with LDH and WST1 assays, respectively. The synthesized silica/titania antibacterial agent also exhibits pronounced photoinduced inactivation of the bacterial growth under the artificial visible and UV light irritation in respect to the commercial catalyst. Additionally, mesoporous silica/titania nanotubes were characterized in details by means of high resolution transmission electron microscopy (HR-TEM), XRD and BET Isotherm.
authors.affiliation: Centre of Knowledge Based Nanomaterials and Technologies, Institute of Chemical and Environment Engineering, West Pomeranian University of Technology Szczecin, Szczecin, Poland, kcendrowski@zut.edu.pl.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Cendrowski
authors.forename: Magdalena
authors.initials: M
authors.lastname: Peruzynska
authors.forename: Agata
authors.initials: A
authors.lastname: Markowska-Szczupak
authors.forename: Xuecheng
authors.initials: X
authors.lastname: Chen
authors.forename: Anna
authors.initials: A
authors.lastname: Wajda
authors.forename: Joanna
authors.initials: J
authors.lastname: Lapczuk
authors.forename: Mateusz
authors.initials: M
authors.lastname: Kurzawski
authors.forename: Ryszard J
authors.initials: RJ
authors.lastname: Kalenczuk
authors.forename: Marek
authors.initials: M
authors.lastname: Drozdzik
authors.forename: Ewa
authors.initials: E
authors.lastname: Mijowska
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology* / Q000633:toxicity; D002460:Cell Line; D004926:Escherichia coli / Q000187:drug effects; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry*; D043942:Nanotubes / Q000737:chemistry*; D055668:Photochemical Processes; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*; D014025:Titanium / Q000737:chemistry* / Q000494:pharmacology* / Q000633:toxicity
chemical_list: D000900:Anti-Bacterial Agents; C009495:titanium dioxide; D012822:Silicon Dioxide; D014025:Titanium