title: The effect of fluoxetine on ischemia-reperfusion after aortic surgery in a rat model.
abstract: BACKGROUND
  Aortic ischemia-reperfusion (IR) is an important factor in the development of postoperative acute lung injury after abdominal aortic surgery. The aim of the present study was to examine the effect of fluoxetine (Flx), a selective serotonin reuptake inhibitor widely used as a preoperative anxiolytic, on lung injury induced by abdominal aortic IR in rats.
  
  
  METHODS
  Wistar rats were randomized into three groups (n = 7 per group): (1) control (sham laparotomy); (2) IR without Flx (60-min ischemia and 120-min reperfusion); (3) IR with Flx (Flx + IR) (Flx 20 mg/kg/d, intraperitoneally for 3 d before surgery). Lung tissue samples and bronchoalveolar lavage (BAL) were obtained for biochemical analysis of oxidative status. Ischemia-modified albumin (IMA) level and protein concentrations in BAL and lung wet to dry weight ratios were determined. Histologic evaluation of the lung tissues was also performed.
  
  
  RESULTS
  IR without Flx led to significant increase in lipid hydroperoxide, malondialdehyde, and pro-oxidant-antioxidant balance and decrease in superoxide dismutase, glutathione, and ferric reducing antioxidant power activities (P < 0.05 versus control), whereas Flx was able to restore these parameters (P > 0.05 versus control) and decrease IMA level (P < 0.01 versus control) and protein concentration (P < 0.05 versus control) in BAL and wet to dry lung weight ratio. Histologic evaluation showed that Flx attenuated the morphologic changes associated with lung injury.
  
  
  CONCLUSIONS
  The results indicate that Flx confers protection against aortic IR-induced lung oxidative stress and cellular integrity. IMA levels in BAL may be used as a follow-up marker for the efficacy of treatment in lung injury.
authors.affiliation: Cerrahpasa Medical Faculty, Department of Physiology, Istanbul University, Istanbul, Turkey.
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Guner
authors.affiliation: Cerrahpasa Medical Faculty, Department of Physiology, Istanbul University, Istanbul, Turkey.
authors.forename: Muhittin O
authors.initials: MO
authors.lastname: Yaman
authors.affiliation: Science Faculty, Department of Biology, Istanbul University, Istanbul, Turkey.
authors.forename: Ugur
authors.initials: U
authors.lastname: Aksu
authors.affiliation: Istanbul Medical Faculty, Istanbul University, Istanbul, Turkey.
authors.forename: Duygu
authors.initials: D
authors.lastname: Uzun
authors.affiliation: Cerrahpasa Medical Faculty, Department of Biochemistry, Istanbul University, Istanbul, Turkey.
authors.forename: Hayriye
authors.initials: H
authors.lastname: Erman
authors.affiliation: Science Faculty, Department of Biology, Istanbul University, Istanbul, Turkey.
authors.forename: Meliha
authors.initials: M
authors.lastname: Inceli
authors.affiliation: Cerrahpasa Medical Faculty, Department of Biochemistry, Istanbul University, Istanbul, Turkey.
authors.forename: Remisa
authors.initials: R
authors.lastname: Gelisgen
authors.affiliation: Cerrahpasa Medical Faculty, Department of Physiology, Istanbul University, Istanbul, Turkey.
authors.forename: Nermin
authors.initials: N
authors.lastname: Yelmen
authors.affiliation: Cerrahpasa Medical Faculty, Department of Biochemistry, Istanbul University, Istanbul, Turkey.
authors.forename: Hafize
authors.initials: H
authors.lastname: Uzun
authors.affiliation: Cerrahpasa Medical Faculty, Department of Physiology, Istanbul University, Istanbul, Turkey. Electronic address: sahing@istanbul.edu.tr.
authors.forename: Gulderen
authors.initials: G
authors.lastname: Sahin
mesh_terms: D055371:Acute Lung Injury / Q000209:etiology / Q000517:prevention & control*; D000818:Animals; D018687:Antidepressive Agents, Second-Generation / Q000627:therapeutic use*; D001012:Aorta, Abdominal / Q000601:surgery*; D015415:Biomarkers / Q000032:analysis; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D004195:Disease Models, Animal; D005473:Fluoxetine / Q000627:therapeutic use*; D008297:Male; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000517:prevention & control*; D012709:Serum Albumin / Q000032:analysis; D000075462:Serum Albumin, Human; D014656:Vascular Surgical Procedures / Q000009:adverse effects
chemical_list: D018687:Antidepressive Agents, Second-Generation; D015415:Biomarkers; D012709:Serum Albumin; C561522:ischemia-modified albumin; D005473:Fluoxetine; D000075462:Serum Albumin, Human
keywords: Abdominal aorta; Fluoxetine; Ischemia-modified albumin; Ischemia–reperfusion; Lung injury; Reactive oxygen species