title: Effects of Teratogenic Drugs on CYP1A1 Activity in Differentiating Rat Embryo Cells.
abstract: CYP1A1, a P450 isoenzyme, is involved in the phase I xenobiotic metabolism including teratogen drugs. In the present study, the ability of teratogens to elevate the embryonic expression of CYP1A1 was examined. Micromass cell cultures prepared from day 13 rat embryo limb buds (LB). LB cells were cultivated and exposed for 5 days to retinoic acid (RA), hydrocortisone (HC), caffeine (CA) and quinine (QN). CYP1A1 protein expression and activity were measured using immunofluorescence staining and ethoxyresorufin O-deethylation (EROD) assay, respectively. The EROD activity increased significantly following LB cells exposure to RA and HC (p<0.05) but the expression of CYP1A1 protein was reduced by these drugs, whereas the expression of CYP1A1 protein and EROD activity decreased significantly following the addition of CA and QN (p<0.05, p<0.01). Our findings show that studied teratogens have potency to increase CYP1A1 activity.
authors.affiliation: Department of Toxicology and Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Gh S
authors.initials: GS
authors.lastname: Tayeboon
authors.affiliation: Department of Toxicology and Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: S N
authors.initials: SN
authors.lastname: Ostad
authors.affiliation: Department of Biology, Payame noor University, Tehran, Iran.
authors.forename: S
authors.initials: S
authors.lastname: Nasri
authors.affiliation: Department of Toxicology and Pharmacology, Faculty of Pharmacy, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: A
authors.initials: A
authors.lastname: Nili-Ahmadabadi
authors.affiliation: Department of Toxicology and Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: F
authors.initials: F
authors.lastname: Tavakoli
authors.affiliation: Department of Toxicology and Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: O
authors.initials: O
authors.lastname: Sabzevari
mesh_terms: D000818:Animals; D002110:Caffeine / Q000494:pharmacology; D002454:Cell Differentiation; D002478:Cells, Cultured; D019363:Cytochrome P-450 CYP1A1 / Q000096:biosynthesis / Q000378:metabolism*; D004622:Embryo, Mammalian / Q000166:cytology* / Q000378:metabolism; D006854:Hydrocortisone / Q000494:pharmacology; D018878:Limb Buds / Q000166:cytology; D011803:Quinine / Q000494:pharmacology; D051381:Rats; D013723:Teratogens / Q000494:pharmacology*; D014212:Tretinoin / Q000494:pharmacology
chemical_list: D013723:Teratogens; D002110:Caffeine; D014212:Tretinoin; D011803:Quinine; D019363:Cytochrome P-450 CYP1A1; D006854:Hydrocortisone