title: Determination of swertianolin in rat plasma by LC-MS/MS and its application to a pharmacokinetic study.
abstract: A sensitive and rapid LC-MS/MS method has been developed and validated for quantifying swertianolin in rat plasma using rutin as an internal standard (IS). Following liquid-liquid extraction with ethyl acetate, chromatographic separation for swertianolin was achieved on a C18 column with a gradient elution using 0.1% formic acid as mobile phase A and acetonitrile as mobile phase B at a flow rate of 0.3 mL/min. The detection was performed on a tandem mass spectrometer using multiple reaction monitoring via an electrospray ionization source and operating in the negative ionization mode. The optimized mass transition ion pairs (m/z) for quantitation were 435.1/272.0 for swertianolin and 609.2/300.1 for IS. The lower limit of quantitation was 0.5 ng/mL within a linear range of 0.5-500 ng/mL. Intra-day and inter-day precision was less than 6.8%. The accuracy was in the range of -13.9 to 12.0%. The mean recovery of swertianolin was >66.7%. The proposed method was successfully applied in evaluating the pharmacokinetics of swertianolin after an oral dose of 50 mg/kg Swertia mussotii extract in rats.
authors.affiliation: Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, China.
authors.forename: Jun
authors.initials: J
authors.lastname: He
authors.forename: Chengwang
authors.initials: C
authors.lastname: Tian
authors.forename: Huizi
authors.initials: H
authors.lastname: Ouyang
authors.forename: Tiwalade A
authors.initials: TA
authors.lastname: Adelakun
authors.forename: Bin
authors.initials: B
authors.lastname: Yu
authors.forename: Yanxu
authors.initials: Y
authors.lastname: Chang
authors.forename: Guixiang
authors.initials: G
authors.lastname: Pan
authors.forename: Linghuo
authors.initials: L
authors.lastname: Jiang
authors.forename: Xiumei
authors.initials: X
authors.lastname: Gao
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D005960:Glucosides / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D016014:Linear Models; D059625:Liquid-Liquid Extraction / Q000379:methods; D008297:Male; D010936:Plant Extracts / Q000008:administration & dosage; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012431:Rutin; D029967:Swertia / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D044004:Xanthones / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics
chemical_list: D005960:Glucosides; D010936:Plant Extracts; D044004:Xanthones; D012431:Rutin
keywords: LC-MS/MS; pharmacokinetics; swertianolin