title: Western blotting using chemiluminescent substrates.
abstract: Western blotting is a powerful and commonly used tool to identify and quantify a specific protein in a complex mixture (Towbin et al., 1979). The technique enables indirect detection of protein samples immobilized on a nitrocellulose or polyvinylidene fluoride (PVDF) membrane.
authors.affiliation: Thermo Fisher Scientific, Rockford, IL, USA. Electronic address: alice.schaffer@thermofisher.com.
authors.forename: Alice
authors.initials: A
authors.lastname: Alegria-Schaffer
mesh_terms: D015153:Blotting, Western / Q000295:instrumentation / Q000379:methods*; D003101:Collodion; D006735:Horseradish Peroxidase / Q000737:chemistry / Q000378:metabolism; D008163:Luminescent Measurements / Q000379:methods*; D008165:Luminol / Q000737:chemistry; D008567:Membranes, Artificial; D011145:Polyvinyls; D011506:Proteins / Q000032:analysis*; D005465:Fluorocarbon Polymers
chemical_list: D003101:Collodion; D006735:Horseradish Peroxidase; D008165:Luminol; D008567:Membranes, Artificial; D011145:Polyvinyls; D011506:Proteins; C024865:polyvinylidene fluoride; D005465:Fluorocarbon Polymers
keywords: Horseradish peroxidase (HRP); Nitrocellulose membrane; Polyvinylidene fluoride (PVDF) membrane; Protein transfer; SDS-PAGE; Western blotting