title: Evaluation of rhBMP-2 and bone marrow derived stromal cell mediated bone regeneration using transgenic fluorescent protein reporter mice.
abstract: The aim of the study is use of transgenic fluorescent protein reporter mouse models to understand the cellular processes in recombinant human bone morphogenetic protein-2 (rhBMP-2) mediated bone formation. Bilateral parietal calvarial bone defects in Col3.6Topaz transgenic fluorescent osteoblast reporter mouse were used to understand the bone formation in the presence and absence of rhBMP2 and/or Col3.6Cyan bone marrow derived stromal cells (BMSCs), using collagen-hydroxyapatite matrix (Healos) as a biomaterial. The bone regeneration was not confined to the site of BMP-2 implantation and significant bone formation was observed in the neighboring defect site. Osteogenic cellular activity with overlying alizarin complexone staining was observed in both the defects indicating host cell induced mineralization. However, implantation of BMSCs along with rhBMP-2 demonstrated a donor cell derived bone formation. The presence of rhBMP-2 did not support host cell recruitment in the presence of donor cells. This study demonstrates the potential of multiple fluorescent reporters to understand the cellular processes involved in the bone regeneration process using biomaterials, growth factors, and/or stem cells.
authors.affiliation: Department of Orthopedic Surgery, University of Connecticut Health Center, Farmington, Connecticut, 06032; Institute for Regenerative Engineering, The Raymond Beverly Sackler Center for Biomedical, Biological, Physical and Engineering Sciences, University of Connecticut Health Center, Farmington, Connecticut, 06032.
authors.forename: Shalini V
authors.initials: SV
authors.lastname: Gohil
authors.forename: Douglas J
authors.initials: DJ
authors.lastname: Adams
authors.forename: Peter
authors.initials: P
authors.lastname: Maye
authors.forename: David W
authors.initials: DW
authors.lastname: Rowe
authors.forename: Lakshmi S
authors.initials: LS
authors.lastname: Nair
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism*; D016026:Bone Marrow Transplantation; D055396:Bone Morphogenetic Protein 2 / Q000737:chemistry / Q000494:pharmacology*; D001861:Bone Regeneration; D016466:CHO Cells; D018914:Cells, Immobilized / Q000166:cytology / Q000378:metabolism / Q000637:transplantation; D003094:Collagen / Q000494:pharmacology*; D006224:Cricetinae; D003412:Cricetulus; D017886:Durapatite / Q000494:pharmacology*; D049452:Green Fluorescent Proteins / Q000096:biosynthesis; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D010012:Osteogenesis; D011994:Recombinant Proteins / Q000737:chemistry / Q000494:pharmacology; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism / Q000637:transplantation
chemical_list: C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; C485182:Cyan Fluorescent Protein; C583670:Healos; D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins; D003094:Collagen; D017886:Durapatite
keywords: bone morphogenetic proteins; bone regeneration; calvarial bone defect model; fluorescent protein reporter mouse; stem cells