title: Simultaneous multiplane imaging of human ovarian cancer by volume holographic imaging.
abstract: Ovarian cancer is the most deadly gynecologic cancer, a fact which is attributable to poor early detection and survival once the disease has reached advanced stages. Intraoperative laparoscopic volume holographic imaging has the potential to provide simultaneous visualization of surface and subsurface structures in ovarian tissues for improved assessment of developing ovarian cancer. In this ex vivo ovarian tissue study, we assembled a benchtop volume holographic imaging system (VHIS) to characterize the microarchitecture of 78 normal and 40 abnormal tissue specimens derived from ovarian, fallopian tube, uterine, and peritoneal tissues, collected from 26 patients aged 22 to 73 undergoing bilateral salpingo-oophorectomy, hysterectomy with bilateral salpingo-oophorectomy, or abdominal cytoreductive surgery. All tissues were successfully imaged with the VHIS in both reflectance- and fluorescence-modes revealing morphological features which can be used to distinguish between normal, benign abnormalities, and cancerous tissues. We present the development and successful application of VHIS for imaging human ovarian tissue. Comparison of VHIS images with corresponding histopathology allowed for qualitatively distinguishing microstructural features unique to the studied tissue type and disease state. These results motivate the development of a laparoscopic VHIS for evaluating the surface and subsurface morphological alterations in ovarian cancer pathogenesis.
authors.affiliation: University of Arizona, Department of Biomedical Engineering, Tucson, Arizona 85719.
authors.forename: Gabriel V
authors.initials: GV
authors.lastname: Orsinger
authors.affiliation: University of Arizona, Department of Biomedical Engineering, Tucson, Arizona 85719.
authors.forename: Jennifer M
authors.initials: JM
authors.lastname: Watson
authors.affiliation: University of Arizona, Department of Optical Sciences, Tucson, Arizona 85721.
authors.forename: Michael
authors.initials: M
authors.lastname: Gordon
authors.affiliation: University of Arizona, Department of Biomedical Engineering, Tucson, Arizona 85719.
authors.forename: Ariel C
authors.initials: AC
authors.lastname: Nymeyer
authors.affiliation: University of Arizona, Department of Optical Sciences, Tucson, Arizona 85721.
authors.forename: Erich E
authors.initials: EE
authors.lastname: de Leon
authors.affiliation: University of Arizona, Department of Optical Sciences, Tucson, Arizona 85721.
authors.forename: Johnathan W
authors.initials: JW
authors.lastname: Brownlee
authors.affiliation: University of Arizona, College of Medicine, Department of Obstetrics and Gynecology, Tucson, Arizona 85724.
authors.forename: Kenneth D
authors.initials: KD
authors.lastname: Hatch
authors.affiliation: University of Arizona, College of Medicine, Department of Obstetrics and Gynecology, Tucson, Arizona 85724.
authors.forename: Setsuko K
authors.initials: SK
authors.lastname: Chambers
authors.affiliation: University of Arizona, Department of Biomedical Engineering, Tucson, Arizona 85719bUniversity of Arizona, Department of Optical Sciences, Tucson, Arizona 85721dUniversity of Arizona, Electrical and Computer Engineering Department, Tucson, Arizona 85721.
authors.forename: Jennifer K
authors.initials: JK
authors.lastname: Barton
authors.affiliation: University of Arizona, Department of Optical Sciences, Tucson, Arizona 85721dUniversity of Arizona, Electrical and Computer Engineering Department, Tucson, Arizona 85721.
authors.forename: Raymond K
authors.initials: RK
authors.lastname: Kostuk
authors.affiliation: University of Arizona, Department of Biomedical Engineering, Tucson, Arizona 85719.
authors.forename: Marek
authors.initials: M
authors.lastname: Romanowski
mesh_terms: D000328:Adult; D000368:Aged; D005187:Fallopian Tubes / Q000033:anatomy & histology / Q000473:pathology; D005260:Female; D006651:Histocytochemistry / Q000379:methods*; D006696:Holography / Q000379:methods*; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008875:Middle Aged; D061848:Optical Imaging / Q000379:methods*; D010051:Ovarian Neoplasms / Q000473:pathology*; D010053:Ovary / Q000033:anatomy & histology / Q000473:pathology; D055815:Young Adult