title: Genome-wide miRNA profiling of villus and decidua of recurrent spontaneous abortion patients.
abstract: MicroRNAs (miRNAs) are non-coding RNA molecules of about 22 nucleotides that involved in post-transcriptional gene regulation. Evidence indicates that miRNAs play essential roles in endometriosis, pre-eclampsia, infertility and other reproductive system diseases. However, whether miRNAs are involved in recurrent spontaneous abortion (RSA) is unclear. In this work, we analysed the miRNA expression profiles in six pairs of villus or decidua from RSA patients and normal pregnancy (NP) women using a human miRNA microarray. Some of the chip results were confirmed by RT-qPCR. In the villi of RSA patients, expression of hsa-miR-184, hsa-miR-187 and hsa-miR-125b-2 was significantly higher, while expression of hsa-miR-520f, hsa-miR-3175 and hsa-miR-4672 was significantly lower, comparing with those of NP control. As well, a total of five miRNAs (hsa-miR-517c, hsa-miR-519a-1, hsa-miR-522, hsa-miR-520h and hsa-miR-184) were upregulated in the decidua of RSA patients. The target genes of these differentially expressed miRNAs were predicted by miRWalk, and we speculate a network of miRNA regulating RSA by target genes function on adhesion, apoptosis and angiogenesis. Our study may help clarify the molecular mechanisms which are involved in the progression of RSA, and provide a reference for future research.
authors.affiliation: Institutes of Biology and Medical SciencesSoochow University, Suzhou, Jiangsu Province 215123, People's Republic of ChinaLaboratory for Reproductive ImmunologyHospital and Institute of Obstetrics and Gynecology, Fudan University Shanghai Medical College, No. 413, Zhaozhou Road, Shanghai 200011, People's Republic of China.
authors.forename: Fulu
authors.initials: F
authors.lastname: Dong
authors.affiliation: Institutes of Biology and Medical SciencesSoochow University, Suzhou, Jiangsu Province 215123, People's Republic of ChinaLaboratory for Reproductive ImmunologyHospital and Institute of Obstetrics and Gynecology, Fudan University Shanghai Medical College, No. 413, Zhaozhou Road, Shanghai 200011, People's Republic of China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Institutes of Biology and Medical SciencesSoochow University, Suzhou, Jiangsu Province 215123, People's Republic of ChinaLaboratory for Reproductive ImmunologyHospital and Institute of Obstetrics and Gynecology, Fudan University Shanghai Medical College, No. 413, Zhaozhou Road, Shanghai 200011, People's Republic of China.
authors.forename: Fei
authors.initials: F
authors.lastname: Xia
authors.affiliation: Institutes of Biology and Medical SciencesSoochow University, Suzhou, Jiangsu Province 215123, People's Republic of ChinaLaboratory for Reproductive ImmunologyHospital and Institute of Obstetrics and Gynecology, Fudan University Shanghai Medical College, No. 413, Zhaozhou Road, Shanghai 200011, People's Republic of China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Institutes of Biology and Medical SciencesSoochow University, Suzhou, Jiangsu Province 215123, People's Republic of ChinaLaboratory for Reproductive ImmunologyHospital and Institute of Obstetrics and Gynecology, Fudan University Shanghai Medical College, No. 413, Zhaozhou Road, Shanghai 200011, People's Republic of China.
authors.forename: Sidong
authors.initials: S
authors.lastname: Xiong
authors.affiliation: Institutes of Biology and Medical SciencesSoochow University, Suzhou, Jiangsu Province 215123, People's Republic of ChinaLaboratory for Reproductive ImmunologyHospital and Institute of Obstetrics and Gynecology, Fudan University Shanghai Medical College, No. 413, Zhaozhou Road, Shanghai 200011, People's Republic of China j_pzhang@suda.edu.cn jinliping2012@gmail.com.
authors.forename: Liping
authors.initials: L
authors.lastname: Jin
authors.affiliation: Institutes of Biology and Medical SciencesSoochow University, Suzhou, Jiangsu Province 215123, People's Republic of ChinaLaboratory for Reproductive ImmunologyHospital and Institute of Obstetrics and Gynecology, Fudan University Shanghai Medical College, No. 413, Zhaozhou Road, Shanghai 200011, People's Republic of China j_pzhang@suda.edu.cn jinliping2012@gmail.com.
authors.forename: Jinping
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000022:Abortion, Spontaneous / Q000235:genetics* / Q000473:pathology / Q000503:physiopathology; D000328:Adult; D016022:Case-Control Studies; D002824:Chorionic Villi / Q000737:chemistry* / Q000473:pathology / Q000503:physiopathology; D016000:Cluster Analysis; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods*; D005786:Gene Expression Regulation; D005819:Genetic Markers; D005865:Gestational Age; D006801:Humans; D035683:MicroRNAs / Q000032:analysis*; D020411:Oligonucleotide Array Sequence Analysis; D010920:Placenta / Q000737:chemistry* / Q000473:pathology / Q000503:physiopathology; D011247:Pregnancy
chemical_list: D005819:Genetic Markers; D035683:MicroRNAs