title: The impact of mesh fixation with a collagen-fibrin sealant in a murine ventral hernia model.
abstract: BACKGROUND
  Pain and adhesions represent the challenge in hernia surgery.
  
  
  OBJECTIVE
  To investigate mesh fixation and adhesion prevention with a collagen-fibrin sealant.
  
  
  METHODS
  Twenty-seven male Sprague-Dawley rats were operated twice, to create and repair 2 ventral hernias. Mesh fixation was with collagen-fibrin sealant on 1 side (group I), whereas an additional peritoneal suture was added in group II. On day 60 animals were killed and mesh migration, integration and number, grade and location of adhesions noted.
  
  
  RESULTS
  Migration occurred in 12 (44.4%) in group 1 and 3 (11.1%) in group 2, P=0.023. Adhesions developed to 18 (33.3%) meshes. There was no difference in adhesion grade or area for mesh center or edge between the groups (P=0.735 and P=0.829, respectively). Median adhesion grade for mesh center was 1 and edge 3 (range, 0 to 4), P=0.005 and P=0.001, respectively. Granuloma formation was noted in 8 (18.6%) animals; only with suture-fixed mesh.
  
  
  CONCLUSIONS
  Mesh fixation with fibrin sealant is not satisfactory, however, adhesion prevention seems to be; adhesions to the edge of the mesh are most severe.
authors.affiliation: Departments of *Pathology §Digestive Surgery, Akershus University Hospital, University of Oslo, Lorenskog †Laboratory Animal Unit, Norwegian School of Veterinary Science, Oslo ‡Department of Gastrointestinal Surgery, Vestfold Hospital, Tonsberg, Norway.
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.forename: Kristine
authors.initials: K
authors.lastname: Eraker-Aasland Hansen
authors.forename: Milan
authors.initials: M
authors.lastname: Spasojevic
authors.forename: Jens-Marius
authors.initials: JM
authors.lastname: Naesgaard
authors.forename: Dejan
authors.initials: D
authors.lastname: Ignjatovic
mesh_terms: D000818:Animals; D015718:Fibrin Tissue Adhesive / Q000627:therapeutic use*; D006555:Hernia, Ventral / Q000601:surgery*; D008297:Male; D011182:Postoperative Care; D017207:Rats, Sprague-Dawley; D013526:Surgical Mesh; D016896:Treatment Outcome
chemical_list: D015718:Fibrin Tissue Adhesive