title: Inhibition of Newcastle disease virus replication by lentivirus-mediated RNA interference.
abstract: Small interfering RNA (siRNA)-induced RNA degradation can specifically inhibit viral infection and has been extensively investigated for its efficacy as an antiviral therapeutic approach. In this study we constructed a lentivirus vector carrying a U6-short hairpin RNA expression cassette to express siRNAs in vero cells. The lentivirus vector also expressed an enhanced green fluorescence protein as a reporter. Stable siRNA-expressing cell lines were successfully established, and the inhibition efficiencies of rationally designed siRNAs targeting conserved genomic regions of the Newcastle disease virus, an important disease of poultry world wide, were assessed. Our results showed that siRNAs targeting the nucleoprotein and matrix gene potently inhibited viral replication. Our study indicates that lentivirus-mediated delivery of siRNA and the resulting gene silencing can be used to study the functions of genes in viral replication and may have a potential transgenic antiviral application in poultry.
authors.forename: Ze
authors.initials: Z
authors.lastname: Wang
authors.forename: Zhuang
authors.initials: Z
authors.lastname: Ding
authors.forename: Chan
authors.initials: C
authors.lastname: Ding
authors.forename: Shengqing
authors.initials: S
authors.lastname: Yu
authors.forename: Yuan
authors.initials: Y
authors.lastname: Dang
authors.forename: Yupei
authors.initials: Y
authors.lastname: Guo
authors.forename: Jianxin
authors.initials: J
authors.lastname: Yang
authors.forename: Qingfeng
authors.initials: Q
authors.lastname: Meng
authors.forename: Jinhua
authors.initials: J
authors.lastname: Liu
authors.forename: Yanlong
authors.initials: Y
authors.lastname: Cong
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000378:metabolism; D002522:Chlorocebus aethiops; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D016086:Lentivirus / Q000235:genetics* / Q000378:metabolism; D009522:Newcastle disease virus / Q000235:genetics* / Q000378:metabolism; D019590:Nucleocapsid Proteins / Q000235:genetics* / Q000378:metabolism; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics* / Q000378:metabolism; D014709:Vero Cells; D014763:Viral Matrix Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D000998:Antiviral Agents; D019590:Nucleocapsid Proteins; D034741:RNA, Small Interfering; D014763:Viral Matrix Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins