title: Linc00963: a novel, long non-coding RNA involved in the transition of prostate cancer from androgen-dependence to androgen-independence.
abstract: Whole genome transcriptomic analyses have identified a large number of long non-coding RNAs (lncRNAs), many of which are involved in a variety of biological functions. However, their functions and molecular mechanisms associated with prostate cancer (PCa) progression to a virulent and androgen-independent (AI) form remain elusive. Herein, we investigated the lncRNA expression profiles of the indolent, androgen-dependent (AD) LNCaP cell line to the aggressive metastatic, AI C4-2 cell line using microarray technology. The differentially expressed lncRNAs and genes were identified by microarray technology and the association in cis or in trans was analyzed to find potential lncRNA target genes. Expression of candidate lncRNAs and putative targets was evaluated by real-time quantitative reverse-transcription polymerase chain reaction (qRT-PCR). The functions of linc00963 on cell proliferation, apoptosis, migration and invasion were evaluated by a knockdown strategy in vitro using MTT, flow cytometric analysis and transwell chamber assays. lncRNAs (n=134) were differentially expressed (FDR<0.001 and fold change≥2) between the LNCaP and C4-2 cell lines. Linc00963 was upregulated most obviously evaluated by qRT-PCR. Knockdown of linc00963 attenuated C4-2 cell proliferation, motility, invasion ability, the expression of EGFR and phosphorylation levels of AKT, and promoted cell apoptosis. Linc00963 was involved in the prostate cancer transition from androgen-dependent to androgen-independent and metastasis via the EGFR signaling pathway.
authors.affiliation: Department of Oncology, the First Affiliated Hospital, Xi'an Jiaotong University of Medical College, Xi'an 710061, P.R. China.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Oncology, the First Affiliated Hospital, Xi'an Jiaotong University of Medical College, Xi'an 710061, P.R. China.
authors.forename: Suxia
authors.initials: S
authors.lastname: Han
authors.affiliation: Department of Oncology, the First Affiliated Hospital, Xi'an Jiaotong University of Medical College, Xi'an 710061, P.R. China.
authors.forename: Guihua
authors.initials: G
authors.lastname: Jin
authors.affiliation: Department of Oncology, the First Affiliated Hospital, Xi'an Jiaotong University of Medical College, Xi'an 710061, P.R. China.
authors.forename: Xia
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Department of Oncology, the First Affiliated Hospital, Xi'an Jiaotong University of Medical College, Xi'an 710061, P.R. China.
authors.forename: Meng
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Oncology, the First Affiliated Hospital, Xi'an Jiaotong University of Medical College, Xi'an 710061, P.R. China.
authors.forename: Xia
authors.initials: X
authors.lastname: Ying
authors.affiliation: Department of Oncology, the First Affiliated Hospital, Xi'an Jiaotong University of Medical College, Xi'an 710061, P.R. China.
authors.forename: Le
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Oncology, the First Affiliated Hospital, Xi'an Jiaotong University of Medical College, Xi'an 710061, P.R. China.
authors.forename: Huili
authors.initials: H
authors.lastname: Wu
authors.affiliation: Department of Oncology, the First Affiliated Hospital, Xi'an Jiaotong University of Medical College, Xi'an 710061, P.R. China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhu
mesh_terms: D017209:Apoptosis; D002460:Cell Line; D002465:Cell Movement; D049109:Cell Proliferation; D066246:ErbB Receptors / Q000037:antagonists & inhibitors* / Q000235:genetics* / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D008297:Male; D009376:Neoplasms, Hormone-Dependent / Q000235:genetics* / Q000473:pathology; D010766:Phosphorylation; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology; D064129:Prostatic Neoplasms, Castration-Resistant / Q000235:genetics* / Q000473:pathology; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D062085:RNA, Long Noncoding / Q000235:genetics* / Q000378:metabolism
chemical_list: D062085:RNA, Long Noncoding; C512478:EGFR protein, human; D066246:ErbB Receptors; D051057:Proto-Oncogene Proteins c-akt