title: Effectiveness of halo-tolerant, auxin producing Pseudomonas and Rhizobium strains to improve osmotic stress tolerance in mung bean (Vigna radiata L.).
abstract: Halo-tolerant, auxin producing bacteria could be used to induce salt tolerance in plants. A number of Rhizobium and auxin producing rhizobacterial strains were assessed for their ability to tolerate salt stress by conducting osmoadaptation assay. The selected strains were further screened for their ability to induce osmotic stress tolerance in mung bean seedlings under salt-stressed axenic conditions in growth pouch/jar trials. Three most effective strains of Rhizobium and Pseudomonas containing ACC-deaminase were evaluated in combination, for their ability to induce osmotic stress tolerance in mung bean at original, 4, and 6 dS m(-1) under axenic conditions. Results showed that sole inoculation of Rhizobium and Pseudomonas strains improved the total dry matter up to 1.4, and 1.9 fold, respectively, while the increase in salt tolerance index was improved up to 1.3 and 2.0 fold by the Rhizobium and Pseudomonas strains, respectively. However, up to 2.2 fold increase in total dry matter and salt tolerance index was observed due to combined inoculation of Rhizobium and Pseudomonas strains. So, combined application of Rhizobium and Pseudomonas strains could be explored as an effective strategy to induce osmotic stress tolerance in mung bean.
authors.affiliation: Institute of Soil and Environmental Sciences, University of Agriculture, Faisalabad, Pakistan. ; University College of Agriculture and Environmental Sciences, The Islamia University of Bahawalpur, Bahawalpur, Pakistan.
authors.forename: Maqshoof
authors.initials: M
authors.lastname: Ahmad
authors.affiliation: Institute of Soil and Environmental Sciences, University of Agriculture, Faisalabad, Pakistan.
authors.forename: Zahir A
authors.initials: ZA
authors.lastname: Zahir
authors.affiliation: Pesticide Quality Control Laboratory, Bahawalpur, Pakistan.
authors.forename: Farheen
authors.initials: F
authors.lastname: Nazli
authors.affiliation: Institute of Soil and Environmental Sciences, University of Agriculture, Faisalabad, Pakistan.
authors.forename: Fareeha
authors.initials: F
authors.lastname: Akram
authors.affiliation: Institute of Soil and Environmental Sciences, University of Agriculture, Faisalabad, Pakistan.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Arshad
authors.affiliation: Institute of Soil and Environmental Sciences, University of Agriculture, Faisalabad, Pakistan.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Khalid
mesh_terms: D007887:Fabaceae / Q000382:microbiology* / Q000502:physiology*; D007210:Indoleacetic Acids / Q000378:metabolism*; D009997:Osmotic Pressure; D011549:Pseudomonas / Q000254:growth & development*; D012231:Rhizobium / Q000254:growth & development*; D012988:Soil Microbiology; D013312:Stress, Physiological
chemical_list: D007210:Indoleacetic Acids
keywords: ACC-deaminase; Pseudomonas; Rhizobium; osmotic stress; salt tolerance index