title: Is capillary electrophoresis on microchip devices able to genotype uridine diphosphate glucuronosyltransferase 1A1 TATA-box polymorphisms?
abstract: In this commentary, we focused our attention on capillary electrophoresis. It achieves the efficient separation of molecular species by the application of high voltages to samples in solution. Actually, capillary electrophoresis can be performed on microchip devices, based on an automated and miniaturized electrophoresis system, based on lab-on-a-chip technology. By this technology it is possible to separate nucleic acid fragments (DNA or RNA) with respect to sizing accuracy and sizing resolution. Currently, two automated capillary electrophoresis on microchips devices are available: the Agilent 2100 Bioanalyzer and the Experion™ Automated Electrophoresis System. In this study, we evaluated if the CE is able to distinguish the three uridine diphosphate glucuronosyltransferase 1A1 TATA-box genotypes.
authors.affiliation: Laboratory of Clinical Molecular Diagnostics and Personalized Medicine, Institute of Biochemistry and Clinical Biochemistry, Catholic University, Rome, Italy.
authors.forename: Angelo
authors.initials: A
authors.lastname: Minucci
authors.forename: Giulia
authors.initials: G
authors.lastname: Canu
authors.forename: Maria
authors.initials: M
authors.lastname: De Bonis
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Delibato
authors.forename: Ettore
authors.initials: E
authors.lastname: Capoluongo
mesh_terms: D004247:DNA / Q000737:chemistry / Q000235:genetics; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D005838:Genotype; D014453:Glucuronosyltransferase / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D056656:Lab-On-A-Chip Devices; D011110:Polymorphism, Genetic; D016385:TATA Box; D000099148:UGT1A1 Enzyme
chemical_list: D004247:DNA; D014453:Glucuronosyltransferase; D000099148:UGT1A1 Enzyme
keywords: Capillary electrophoresis; Lab on a chip; UGT1A1 TATA-box polymorphisms