title: SNP rs8099917 in gene IL28B might be associated with risk of chronic infection by HCV but not with response to treatment.
abstract: OBJECTIVE
  The aim of this study was to characterize the genetic profile of patients with chronic hepatitis C virus (HCV) infection relative to polymorphisms rs12979860 and rs8099917 in gene IL28B and the association of those polymorphisms with the response to treatment with pegylated interferon and ribavirin, performed at a reference center in Brazilian Amazonia.
  
  
  METHODS
  A total of 75 individuals with chronic hepatitis C and 98 healthy individuals from both genders over 18 years old were assessed. DNA samples were collected from leukocytes and subjected to real-time polymerase chain reaction to genotype polymorphisms rs12979860 and rs8099917.
  
  
  RESULTS
  Analysis of the allelic and genotypic frequencies of the investigated polymorphisms showed that both groups were in Hardy-Weinberg equilibrium; polymorphism rs12979860 exhibited no significant difference between the groups. For polymorphism rs8099917, allele T was significantly less frequent (P = 0.0195) among the patients (63.3%) than the controls (75.5%), and the patients were 1.7 times as likely to exhibit allele G. No difference in response to treatment was associated with SNP patterns.
  
  
  CONCLUSIONS
  The results suggest a possible association of SNP rs8099917 with higher odds of chronic HCV infection but do not indicate a putative influence of the investigated SNPs on the sustained virologic response.
authors.affiliation: Laboratory of Virology, Institute of Biological Sciences, Federal University of Pará, Guamá 66075-110, Belém, PA, Brazil ; School of Medicine, Institute of Health Sciences, Federal University of Pará, Praça. Camilo Salgado, No. 01, Belém, PA, Brazil.
authors.forename: Simone Regina Souza
authors.initials: SR
authors.lastname: da Silva Conde
authors.affiliation: School of Medicine, Institute of Health Sciences, Federal University of Pará, Praça. Camilo Salgado, No. 01, Belém, PA, Brazil.
authors.forename: Julius Caesar Mendes
authors.initials: JC
authors.lastname: Soares Monteiro
authors.affiliation: School of Medicine, Institute of Health Sciences, Federal University of Pará, Praça. Camilo Salgado, No. 01, Belém, PA, Brazil.
authors.forename: Bruna Tereza
authors.initials: BT
authors.lastname: Silva Dos Santos
authors.affiliation: School of Medicine, Institute of Health Sciences, Federal University of Pará, Praça. Camilo Salgado, No. 01, Belém, PA, Brazil.
authors.forename: Nathália Karla
authors.initials: NK
authors.lastname: Fonseca Filgueiras
authors.affiliation: School of Medicine, Institute of Health Sciences, Federal University of Pará, Praça. Camilo Salgado, No. 01, Belém, PA, Brazil.
authors.forename: Pedro Alves
authors.initials: PA
authors.lastname: de Almeida Lins
authors.affiliation: Laboratory of Virology, Institute of Biological Sciences, Federal University of Pará, Guamá 66075-110, Belém, PA, Brazil.
authors.forename: Felipe
authors.initials: F
authors.lastname: Bonfim Freitas
authors.affiliation: Laboratory of Virology, Institute of Biological Sciences, Federal University of Pará, Guamá 66075-110, Belém, PA, Brazil.
authors.forename: Ednelza
authors.initials: E
authors.lastname: da Silva Graça
authors.affiliation: Service of Anatomic Pathology, School of Medicine, João de Barros Barreto University Hospital, Federal University of Pará, Belém, PA, Brazil.
authors.forename: Sâmia
authors.initials: S
authors.lastname: Demachki
authors.affiliation: Service of Anatomic Pathology, School of Medicine, João de Barros Barreto University Hospital, Federal University of Pará, Belém, PA, Brazil.
authors.forename: Marialva Tereza
authors.initials: MT
authors.lastname: Ferreira de Araújo
authors.affiliation: Laboratory of Virology, Institute of Biological Sciences, Federal University of Pará, Guamá 66075-110, Belém, PA, Brazil.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Ishak
authors.affiliation: Laboratory of Virology, Institute of Biological Sciences, Federal University of Pará, Guamá 66075-110, Belém, PA, Brazil.
authors.forename: Antonio Carlos
authors.initials: AC
authors.lastname: Rosário Vallinoto
mesh_terms: D016022:Case-Control Studies; D005260:Female; D005500:Follow-Up Studies; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D016174:Hepacivirus / Q000502:physiology*; D019698:Hepatitis C, Chronic / Q000188:drug therapy / Q000235:genetics* / Q000821:virology*; D006801:Humans; D007372:Interferons / Q000627:therapeutic use; D007378:Interleukins / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012254:Ribavirin / Q000627:therapeutic use; D012307:Risk Factors; D016896:Treatment Outcome; D000095022:Interferon Lambda
chemical_list: D007372:Interferons; D007378:Interleukins; D012254:Ribavirin; C000624353:interferon-lambda, human; D000095022:Interferon Lambda