title: Efficacy of a live attenuated Escherichia coli O78:K80 vaccine in chickens and turkeys.
abstract: A candidate live vaccine for avian pathogenic Escherichia coli (APEC) was constructed from a virulent field APEC 078 strain by mutation of the aroA gene. The mutant was highly similar to the parent wild-type strain in respect of colony morphology, motility, growth in suspension, hemagglutination, Congo Red binding, HEp-2 cell adhesion, and the elaboration of surface antigens type 1 fimbriae and flagella, although production of curli fimbriae was reduced marginally. The mutant proved avirulent when inoculated into 1-day-old chicks by spray application and when presented again in the drinking water at 7 days of age. Chickens and turkeys vaccinated with an 078 aroA mutant were protected against a challenge at 6 wk of age by virulent APEC strains.
authors.forename: R M
authors.initials: RM
authors.lastname: La Ragione
authors.forename: M J
authors.initials: MJ
authors.lastname: Woodward
authors.forename: M
authors.initials: M
authors.lastname: Kumar
authors.forename: J
authors.initials: J
authors.lastname: Rodenberg
authors.forename: H
authors.initials: H
authors.lastname: Fan
authors.forename: A D
authors.initials: AD
authors.lastname: Wales
authors.forename: K
authors.initials: K
authors.lastname: Karaca
mesh_terms: D000818:Animals; D002645:Chickens; D004926:Escherichia coli / Q000235:genetics / Q000276:immunology*; D004927:Escherichia coli Infections / Q000517:prevention & control / Q000662:veterinary*; D022361:Escherichia coli Vaccines / Q000008:administration & dosage / Q000009:adverse effects*; D010861:Fimbriae, Bacterial / Q000502:physiology*; D005407:Flagella / Q000502:physiology; D011201:Poultry Diseases / Q000517:prevention & control*; D014422:Turkeys; D014613:Vaccines, Attenuated / Q000008:administration & dosage / Q000009:adverse effects
chemical_list: D022361:Escherichia coli Vaccines; D014613:Vaccines, Attenuated