title: Real-time light scattering tracking of gold nanoparticles- bioconjugated respiratory syncytial virus infecting HEp-2 cells.
abstract: Real-time tracking of virus invasion is crucial for understanding viral infection mechanism, which, however, needs simple and efficient labeling chemistry with improved signal-to-noise ratio. For that purpose, herein we investigated the invasion dynamics of respiratory syncytial virus (RSV) through dark-field microscopic imaging (iDFM) technique by using Au nanoparticles (AuNPs) as light scattering labels. RSV, a ubiquitous, non-segmented, pleiomorphic and negative-sense RNA virus, is an important human pathogen in infants, the elderly, and the immunocompromised. In order to label the enveloped virus of paramyxoviridae family, an efficient streptavidin (SA)-biotin binding chemistry was employed, wherein AuNPs and RSV particles modified with SA and biotin, respectively, allowing the AuNP-modified RSVs to maintain their virulence without affecting the native activities of RSV, making the long dynamic visualization successful for the RSV infections into human epidermis larynx carcinoma cells.
authors.affiliation: Key Laboratory of Luminescence and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Pharmaceutical Sciences, Southwest University.
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Wan
authors.affiliation: College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
authors.forename: Lin-Ling
authors.initials: LL
authors.lastname: Zheng
authors.affiliation: Key Laboratory of Luminescence and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Pharmaceutical Sciences, Southwest University.
authors.forename: Peng-Fei
authors.initials: PF
authors.lastname: Gao
authors.affiliation: Key Laboratory of Luminescence and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Pharmaceutical Sciences, Southwest University.
authors.forename: Xiao-Xi
authors.initials: XX
authors.lastname: Yang
authors.affiliation: Key Laboratory of Luminescence and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Pharmaceutical Sciences, Southwest University.
authors.forename: Chun-Mei
authors.initials: CM
authors.lastname: Li
authors.affiliation: College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
authors.forename: Yuan Fang
authors.initials: YF
authors.lastname: Li
authors.affiliation: 1] Key Laboratory of Luminescence and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Pharmaceutical Sciences, Southwest University [2] College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
authors.forename: Cheng Zhi
authors.initials: CZ
authors.lastname: Huang
mesh_terms: D001710:Biotin / Q000378:metabolism; D045744:Cell Line, Tumor; D006046:Gold / Q000378:metabolism*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D018357:Respiratory Syncytial Virus Infections / Q000378:metabolism*; D012136:Respiratory Syncytial Viruses / Q000378:metabolism*; D059629:Signal-To-Noise Ratio; D019809:Streptavidin / Q000378:metabolism
chemical_list: D001710:Biotin; D006046:Gold; D019809:Streptavidin