title: Tamarind kernel powder: a novel agro-residue for the production of cellobiose dehydrogenase under submerged fermentation by Termitomyces clypeatus.
abstract: The study investigates the potential of substitution of the conventional carbohydrate nutrient (cellulose) in media with cheap agro-residues for cellobiose dehydrogenase production by Termitomyces clypeatus (CDHtc) under submerged conditions. Different agro-residues tested for enzyme production were characterized using FTIR and XRD analysis. As CDHtc production was highest with tamarind kernel powder (TKP), it was selected for process optimizations through shake-flask fermentations. The optimized parameters were then applied to batch cultures in a 5 L bioreactor that gave enzyme yield (57.4 U mL⁻¹) similar to that obtained under shake-flask fermentations (57.05 U mL⁻¹). The study also made an attempt to predict CDHtc production with respect to time of fermentation and mycelial growth. The specific growth rate and carrying capacity of the mycelia were also determined, and the values lie in the ranges of 0.024-0.027 h⁻¹ and 7.2-7.1 mg mL⁻¹, respectively.
authors.affiliation: Department of Molecular Biology and Biotechnology, University of Kalyani , Distt: Nadia, West Bengal 741 235, India.
authors.forename: Tanima
authors.initials: T
authors.lastname: Saha
authors.forename: Soumya
authors.initials: S
authors.lastname: Sasmal
authors.forename: Shariful
authors.initials: S
authors.lastname: Alam
authors.forename: Nirmalendu
authors.initials: N
authors.lastname: Das
mesh_terms: D061249:Batch Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D019149:Bioreactors / Q000382:microbiology; D002237:Carbohydrate Dehydrogenases / Q000096:biosynthesis*; D003470:Culture Media / Q000737:chemistry / Q000378:metabolism; D005285:Fermentation; D005656:Fungal Proteins / Q000096:biosynthesis*; D012639:Seeds / Q000737:chemistry / Q000378:metabolism / Q000382:microbiology; D029861:Tamarindus / Q000737:chemistry / Q000378:metabolism* / Q000382:microbiology; D055434:Termitomyces / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D014866:Waste Products / Q000032:analysis
chemical_list: D003470:Culture Media; D005656:Fungal Proteins; D014866:Waste Products; D002237:Carbohydrate Dehydrogenases; C019859:cellobiose-quinone oxidoreductase