title: GPC5, a tumor suppressor, is regulated by miR-620 in lung adenocarcinoma.
abstract: In the current study, a proportion of lung adenocarcinoma was shown to reduce GPC5 expression in the absence of transcriptional silencing of the tumor suppressor gene, GPC5, by aberrant methylation of CpG islands. It was hypothesized that the loss of GPC5 expression is associated with upregulation of miR‑620 in human lung adenocarcinoma tissue compared with the matched normal lung tissue. The downregulation of GPC5 in lung adenocarcinoma cell lines is regulated by miR‑620 through binding of the 3'‑untranslated region. Furthermore, blockage of miR‑620 inhibited the proliferation, migration and invasion of lung adenocarcinoma cells by directly regulating GPC5, and GPC5 knockdown eliminates this phenotype. These results provided a novel insight into the mechanism of miRNA regulation in lung adenocarcinoma.
authors.affiliation: Department of General Thoracic Surgery, Affiliated Hospital of the Logistics University of Chinese People's Armed Police Forces, Tianjin 300162, P.R. China.
authors.forename: Zhengyuan
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Department of General Thoracic Surgery, Affiliated Hospital of the Logistics University of Chinese People's Armed Police Forces, Tianjin 300162, P.R. China.
authors.forename: Chengguang
authors.initials: C
authors.lastname: Han
authors.affiliation: Department of General Thoracic Surgery, Affiliated Hospital of the Logistics University of Chinese People's Armed Police Forces, Tianjin 300162, P.R. China.
authors.forename: Juntao
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of General Thoracic Surgery, Affiliated Hospital of the Logistics University of Chinese People's Armed Police Forces, Tianjin 300162, P.R. China.
authors.forename: Changlei
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of General Thoracic Surgery, Affiliated Hospital of the Logistics University of Chinese People's Armed Police Forces, Tianjin 300162, P.R. China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of General Thoracic Surgery, Affiliated Hospital of the Logistics University of Chinese People's Armed Police Forces, Tianjin 300162, P.R. China.
authors.forename: Liya
authors.initials: L
authors.lastname: Cheng
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D000077192:Adenocarcinoma of Lung; D000328:Adult; D000368:Aged; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D019175:DNA Methylation; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D053673:Glypicans / Q000235:genetics*; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D060787:Neoplasm Grading; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D034622:RNA Interference; D014158:Transcription, Genetic; D025521:Tumor Suppressor Proteins / Q000235:genetics*
chemical_list: C106228:GPC5 protein, human; D053673:Glypicans; C000594137:MIRN620 microRNA, human; D035683:MicroRNAs; D025521:Tumor Suppressor Proteins