title: Effect of hyaluronan on developmental competence and quality of oocytes and obtained blastocysts from in vitro maturation of bovine oocytes.
abstract: The objective of the present study was to evaluate the effect of hyaluronan (HA) during IVM on meiotic maturation, embryonic development, and the quality of oocytes, granulosa cells (GC), and obtained blastocysts. COCs were matured in vitro in control medium and medium with additional 0.035% or 0.07% of exogenous HA. The meiotic maturity did not differ between the analysed groups. The best rate and the highest quality of obtained blastocysts were observed when 0.07% HA was used. A highly significant difference (P < 0.001) was noted in the mean number of apoptotic nuclei per blastocyst and in the DCI between the 0.07% HA and the control blastocysts (P < 0.01). Our results suggest that addition of 0.035% HA and 0.07% HA to oocyte maturation media does not affect oocyte nuclear maturation and DNA fragmentation. However, the addition of 0.07% HA during IVM decreases the level of blastocysts DNA fragmentation. Finally, our results suggest that it may be risky to increase the HA concentration during IVM above 0.07% as we found significantly higher Bax mRNA expression levels in GC cultured with 0.07% HA. The final concentration of HA being supplemented to oocyte maturation media is critical for the success of the IVP procedure.
authors.affiliation: Department of Biotechnology of Animal Reproduction, National Research Institute of Animal Production, Krakowska 1 Street, 32-083 Balice, Kraków, Poland.
authors.forename: Jolanta
authors.initials: J
authors.lastname: Opiela
authors.affiliation: Department of Biotechnology of Animal Reproduction, National Research Institute of Animal Production, Krakowska 1 Street, 32-083 Balice, Kraków, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Romanek
authors.affiliation: Department of Biochemistry and Biotechnology, Poznań University of Life Sciences, Dojazd 11 Street, 60-632 Poznań, Poland ; Institute of Human Genetics, Polish Academy of Sciences, Strzeszyńska 32, 60-479 Poznań, Poland.
authors.forename: Daniel
authors.initials: D
authors.lastname: Lipiński
authors.affiliation: Department of Biotechnology of Animal Reproduction, National Research Institute of Animal Production, Krakowska 1 Street, 32-083 Balice, Kraków, Poland.
authors.forename: Zdzisław
authors.initials: Z
authors.lastname: Smorąg
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D002417:Cattle; D002843:Chromatin / Q000378:metabolism; D018920:Coculture Techniques; D053938:DNA Fragmentation / Q000187:drug effects; D005260:Female; D006107:Granulosa Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006820:Hyaluronic Acid / Q000494:pharmacology*; D020287:In Situ Nick-End Labeling; D066298:In Vitro Techniques; D008540:Meiosis / Q000187:drug effects; D009865:Oocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism
chemical_list: D002843:Chromatin; D012333:RNA, Messenger; D051028:bcl-2-Associated X Protein; D006820:Hyaluronic Acid