title: Strontium does not affect the intrinsic bone quality at tissue and BSU levels in iliac samples from Macaca fascicularis monkeys.
abstract: Our purpose was to evaluate the impact of strontium ranelate (SrRan) on bone mineral quality at both tissue and bone structural unit (BSU) levels. Thirty iliac bone samples (dehydrated then embedded) were taken from monkeys who received 0 (controls), 200, 500 or 1250 mg/kg/day of SrRan for 52 weeks and were sacrificed either at the end of administration (treated animals, n=16) or 10 weeks later (reverse animals, n=14). Degree of mineralization (DMB), heterogeneity index of mineralization (HI), Vickers microhardness (Hv) and focal bone strontium content (BSC) were measured globally at tissue level and focally on the same 923 BSUs. Mineral and collagen characteristics, as well as chemometric analyses were performed on younger and older tissues in cortical bone and cancellous bone in 737 other BSUs. At tissue level, SrRan preserved material properties. At BSU level, BSC increased (significant) dose dependently in treated and reverse animals. DMB and Hv were greater in older than in younger bone in controls and treated animals. In treated animals, DMB was positively correlated with Hv and inversely correlated with the BSC. Thus, younger BSUs were less mineralized and less hard than older BSUs independently from the presence of strontium. Mineral maturity, crystallinity index, mineralization index, carbonation and collagen maturity were not modified by SrRan. Chemometry confirmed the absence of a direct effect of strontium on mineralization. Thus, surrogates of micro- and nano-structural mineral properties were not altered by SrRan and remained at a physiological level.
authors.affiliation: INSERM UMR 1033, F-69008 Lyon, France; Université de Lyon, F-69008 Lyon, France. Electronic address: audrey.doublier@gmail.com.
authors.forename: Audrey
authors.initials: A
authors.lastname: Doublier
authors.affiliation: INSERM UMR 1033, F-69008 Lyon, France; Université de Lyon, F-69008 Lyon, France. Electronic address: delphine.farlay@inserm.fr.
authors.forename: Delphine
authors.initials: D
authors.lastname: Farlay
authors.affiliation: INSERM UMR 1033, F-69008 Lyon, France; Université de Lyon, F-69008 Lyon, France. Electronic address: yohannbala@gmail.com.
authors.forename: Yohann
authors.initials: Y
authors.lastname: Bala
authors.affiliation: INSERM UMR 1033, F-69008 Lyon, France; Université de Lyon, F-69008 Lyon, France. Electronic address: georges.boivin@univ-lyon1.fr.
authors.forename: Georges
authors.initials: G
authors.lastname: Boivin
mesh_terms: D000818:Animals; D015519:Bone Density / Q000187:drug effects*; D050071:Bone Density Conservation Agents / Q000494:pharmacology*; D007085:Ilium / Q000187:drug effects*; D008252:Macaca fascicularis; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D013876:Thiophenes / Q000494:pharmacology*
chemical_list: D050071:Bone Density Conservation Agents; D013876:Thiophenes; C081587:strontium ranelate
keywords: Bone microhardness; Collagen maturity; Mineral characteristics; Monkeys; Secondary mineralization; Strontium