title: Pre-exposure to adenosine, acting via A(2A) receptors on endothelial cells, alters the protein kinase A dependence of adenosine-induced dilation in skeletal muscle resistance arterioles.
abstract: Adenosine (ADO) is an endogenous vasodilatory purine widely recognized to be a significant contributor to functional hyperaemia. Despite this, many aspects of the mechanisms by which ADO induces dilation in small resistance arterioles are not established, or appear contradictory. These include: identification of the primary receptor subtype; its location on endothelial (EC) or vascular smooth muscle cells; whether ADO acts on KATP channels in these resistance vessels; and the contribution of cAMP/protein kinase A (PKA) signalling to the response. In intravital microscopy studies of intact or EC-denuded skeletal muscle arterioles, we show that ADO acts via A2A receptors located on ECs to produce vasodilation via activation of KATP channels located on vascular smooth muscle cells. Importantly, we found that the signalling pathway involves cAMP as expected, but that a requirement for PKA activation is demonstrable only if the vessel is not pre-exposed to ADO. That is, PKA-dependent signalling varies with pre-exposure to ADO. Further, we show that PKA activation alone is not sufficient to dilate these arterioles; an additional EC calcium-dependent signalling mechanism is required for vasodilation to ADO. The ability of arterioles in situ to respond to occupancy of a specific receptor by utilizing different cell signalling pathways under different conditions to produce the same response allows the arteriole to respond to key homeostatic requirements using more than a single signalling mechanism. Clearly, this is likely to be physiologically advantageous, but the role for this signalling flexibility in the integrated arteriolar response that underlies functional hyperaemia will require further exploration.
authors.affiliation: Department of Pharmacology and Physiology, University of Rochester, Rochester, NY, USA.
authors.forename: Nir
authors.initials: N
authors.lastname: Maimon
authors.affiliation: Department of Pharmacology and Physiology, University of Rochester, Rochester, NY, USA.
authors.forename: Patricia A
authors.initials: PA
authors.lastname: Titus
authors.affiliation: Department of Pharmacology and Physiology, University of Rochester, Rochester, NY, USA ingrid_sarelius@urmc.rochester.edu.
authors.forename: Ingrid H
authors.initials: IH
authors.lastname: Sarelius
mesh_terms: D000241:Adenosine / Q000502:physiology*; D000818:Animals; D001160:Arterioles / Q000502:physiology*; D002118:Calcium / Q000378:metabolism; D017868:Cyclic AMP-Dependent Protein Kinases / Q000502:physiology; D042783:Endothelial Cells / Q000502:physiology; D004730:Endothelium, Vascular / Q000502:physiology*; D006940:Hyperemia / Q000503:physiopathology; D066298:In Vitro Techniques; D008297:Male; D008810:Mice, Inbred C57BL; D018482:Muscle, Skeletal / Q000098:blood supply / Q000502:physiology; D043705:Receptor, Adenosine A2A / Q000502:physiology*; D014655:Vascular Resistance; D014664:Vasodilation / Q000502:physiology*
chemical_list: D043705:Receptor, Adenosine A2A; D017868:Cyclic AMP-Dependent Protein Kinases; D000241:Adenosine; D002118:Calcium