title: HSV-1 amplicon vectors as genetic vaccines.
abstract: HSV-1 amplicon vectors have been used as platforms for the generation of genetic vaccines against both DNA and RNA viruses. Mice vaccinated with such vectors encoding structural proteins from both foot-and-mouth disease virus and rotavirus were partially protected from challenge with wild-type virus (D'Antuono et al. Vaccine 28: 7363-7372, 2010; Laimbacher et al. Mol Ther 20: 1810-1820, 2012), indicating that HSV-1 amplicon vectors are attractive tools for the development of complex and safe genetic vaccines. This chapter describes the use of HSV-1 amplicon vectors that encode individual or multiple viral structural proteins from a polycistronic transgene cassette in mammalian cells. More precisely, amplicon vectors that encode multiple structural viral proteins support the in situ production of viruslike particles (VLPs) in vector-infected cells. The expression of the viral genes is confirmed by Western blot and immune fluorescence analysis, and the generation of VLPs in vector-infected cells is demonstrated by electron microscopy.
authors.affiliation: Vetsuisse Faculty, Institute of Virology, University of Zurich, Winterthurerstrasse 266a, 8057, Zurich, Switzerland.
authors.forename: Andrea S
authors.initials: AS
authors.lastname: Laimbacher
authors.forename: Cornel
authors.initials: C
authors.lastname: Fraefel
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D005536:Foot-and-Mouth Disease / Q000276:immunology / Q000517:prevention & control / Q000628:therapy; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics / Q000276:immunology; D018259:Herpesvirus 1, Human / Q000235:genetics* / Q000276:immunology; D006801:Humans; D051379:Mice; D008967:Molecular Biology / Q000379:methods*; D012401:Rotavirus / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D012400:Rotavirus Infections / Q000276:immunology / Q000517:prevention & control / Q000628:therapy; D014709:Vero Cells; D015678:Viral Structural Proteins / Q000037:antagonists & inhibitors / Q000276:immunology; D014765:Viral Vaccines / Q000235:genetics* / Q000627:therapeutic use
chemical_list: D015678:Viral Structural Proteins; D014765:Viral Vaccines