title: A simple strategy for glycosyltransferase-catalyzed aminosugar nucleotide synthesis.
abstract: A set of 2-chloro-4-nitrophenyl glucosamino-/xylosaminosides were synthesized and assessed as potential substrates in the context of glycosyltransferase-catalyzed formation of the corresponding UDP/TDP-α-D-glucosamino-/xylosaminosugars and in single-vessel model transglycosylation reactions. This study highlights a robust platform for aminosugar nucleotide synthesis and reveals OleD Loki to be a proficient catalyst for U/TDP-aminosugar synthesis and utilization
authors.forename: Jianjun
authors.initials: J
authors.lastname: Zhang
authors.forename: Shanteri
authors.initials: S
authors.lastname: Singh
authors.forename: Ryan R
authors.initials: RR
authors.lastname: Hughes
authors.forename: Maoquan
authors.initials: M
authors.lastname: Zhou
authors.forename: Manjula
authors.initials: M
authors.lastname: Sunkara
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Morris
authors.forename: Jon S
authors.initials: JS
authors.lastname: Thorson
mesh_terms: D000588:Amines / Q000737:chemistry / Q000378:metabolism*; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000737:chemistry; D002384:Catalysis; D005960:Glucosides / Q000737:chemistry / Q000378:metabolism; D016695:Glycosyltransferases / Q000235:genetics / Q000378:metabolism*; D009596:Nitrophenols / Q000737:chemistry / Q000378:metabolism; D009711:Nucleotides / Q000737:chemistry / Q000378:metabolism*; D015202:Protein Engineering; D013303:Streptomyces antibioticus / Q000201:enzymology* / Q000235:genetics; D013379:Substrate Specificity
chemical_list: D000588:Amines; D002241:Carbohydrates; D005960:Glucosides; D009596:Nitrophenols; D009711:Nucleotides; C024719:4-nitrophenyl; D016695:Glycosyltransferases