title: Excavation of Pid3 orthologs with differential resistance spectra to Magnaporthe oryzae in rice resource.
abstract: Twenty-six orthologs of the rice blast resistance gene Pid3 from cultivated varieties and wild rice accessions distributed in different areas were cloned by allele mining. Sequence analysis showed that while each of the orthologous genes from indica varieties and most wild accessions encodes a complete NBS-LRR protein, each of the proteins encoded by those from japonica varieties and few wild rice accessions presents a premature termination. Eleven of the 26 orthologs were selected for blast resistance testing by transforming into the blast susceptible rice variety TP309, respectively. Inoculation of 23 M. oryzae strains collected from diverse regions of China to the respective transgenic plants revealed that 6 Pid3 orthologs showed susceptible to all the tested strains, while the other 5 orthologs showed differential resistance spectra in a gradually spectrum-widen order as Pid3-W3, Pid3-W4, Pid3-I3, Pid3-W5 and Pid3-I1. Amino acid sequences alignment of these orthologs indicated that the sequence diversities between the blast resistance orthologs were mostly located in the LRR domain such as the substitutions of Q694H,D856H,Q896R,D899E etc. However, the differences between the resistance orthologs and the susceptible ones were mostly located in the NBS domain. The present experiments provide an example of that the ortholog evaluation of plant R genes could be an efficient way to expand the rice blast resistance and some other plant disease resistance as well for breeding.
authors.affiliation: National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing, China; State Key Laboratory of Plant Genomics, National Centre for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Science, Bejing, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Plant Genomics, National Centre for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Science, Bejing, China; Rice Research Institute, Sichuan Agricultural University, Chengdu, China; National Key Laboratory of Hybrid Rice, Changsha, Hunan, China.
authors.forename: Qiming
authors.initials: Q
authors.lastname: Lv
authors.affiliation: Key Laboratory of Molecular Biology and Gene Engineering, College of Life Science, Nanchang University, Nanchang, China.
authors.forename: Junjun
authors.initials: J
authors.lastname: Shang
authors.affiliation: Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, China.
authors.forename: Zhiqian
authors.initials: Z
authors.lastname: Pang
authors.affiliation: State Key Laboratory of Plant Genomics, National Centre for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Science, Bejing, China.
authors.forename: Zhuangzhi
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Plant Genomics, National Centre for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Science, Bejing, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Plant Genomics, National Centre for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Science, Bejing, China.
authors.forename: Guanghuai
authors.initials: G
authors.lastname: Jiang
authors.affiliation: Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Tao
authors.affiliation: National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Plant Genomics, National Centre for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Science, Bejing, China.
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Li
authors.affiliation: State Key Laboratory of Plant Genomics, National Centre for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Science, Bejing, China.
authors.forename: Xianfeng
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Rice Research Institute, Sichuan Agricultural University, Chengdu, China.
authors.forename: Shigui
authors.initials: S
authors.lastname: Li
authors.affiliation: National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing, China.
authors.forename: Jichen
authors.initials: J
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Plant Genomics, National Centre for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Science, Bejing, China.
authors.forename: Lihuang
authors.initials: L
authors.lastname: Zhu
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D001947:Breeding / Q000379:methods; D002681:China; D060467:Disease Resistance / Q000235:genetics*; D017343:Genes, Plant / Q000235:genetics*; D020082:Magnaporthe / Q000235:genetics*; D012275:Oryza / Q000235:genetics*; D010802:Phylogeny; D010935:Plant Diseases / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D010940:Plant Proteins