title: Characterization of hsp70 gene promoter for cis- acting elements in Indian zebu cattle of Hariana breed.
abstract: The promoter region of hsp70 gene was characterized for cis-acting elements in zebu cattle of Hariana breed. The basal regulatory domain of CAAT box identified as CAAT/enhancer binding protein (C/EBP) and CAAT binding transcription factor (CTF) binding sites, as well as GC box identified as sp1 binding site, were localized in at least two regions in the hsp70 gene promoter. A highly conserved heat shock element was found between position -108 to -95, which exactly matched at all eight positions with the consensus sequence. These cis-acting elements were found to be conserved between Holstein-Friesian and studied zebu breed.
authors.affiliation: a National Bureau of Animal Genetic Resources , Karnal , Haryana , India.
authors.forename: Rahul
authors.initials: R
authors.lastname: Behl
authors.forename: Jyotsna
authors.initials: J
authors.lastname: Behl
authors.forename: D K
authors.initials: DK
authors.lastname: Sadana
authors.forename: R K
authors.initials: RK
authors.lastname: Vijh
authors.forename: M S
authors.initials: MS
authors.lastname: Tantia
authors.forename: B K
authors.initials: BK
authors.lastname: Joshi
mesh_terms: D000818:Animals; D001665:Binding Sites / Q000235:genetics; D022762:CCAAT-Enhancer-Binding Proteins / Q000235:genetics; D002417:Cattle; D004247:DNA / Q000032:analysis / Q000235:genetics; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*
chemical_list: D022762:CCAAT-Enhancer-Binding Proteins; D018840:HSP70 Heat-Shock Proteins; D004247:DNA