title: FilmArray® respiratory panel performance in respiratory samples from neonatal care units.
abstract: FilmArray Respiratory Panel (RP) (Idaho Technology, Inc., Salt Lake City, UT, USA) performance was retrospectively evaluated in respiratory samples collected from neonates in 2 reference neonatology units. Using the FilmArray RP assay, 121/152 (79.6%) samples were positive for at least 1 respiratory virus, while 31/152 (20.4%) were negative. FilmArray RP results were concordant in 68/72 (94.4%) respiratory samples tested with laboratory-developed real-time PCR assays, while in 4/72 (5.6%) samples, the FilmArray RP assay detected an additional virus (2 human rhinovirus/enterovirus and 2 bocavirus). In addition, FilmArray RP results for 70 of 80 (87.5%) respiratory samples tested were concordant with the Seegene Seeplex RV15® detection assay (Seegene, Inc., Seoul, South Korea), while 10/80 (12.5%) were discordant. The advantages of the FilmArray RP are the rapid detection of respiratory viruses (1 hour), the wide number of pathogens detectable in a single assay, and the reduced hands-on time.
authors.affiliation: Molecular Virology Unit, Microbiology and Virology Department, Fondazione IRCCS Policlinico San Matteo, 27100, Pavia, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Piralla
authors.affiliation: Virology Unit; Fondazione Cà Granda Ospedale Maggiore, Policlinico, Milano, Italy.
authors.forename: Giovanna
authors.initials: G
authors.lastname: Lunghi
authors.affiliation: Molecular Virology Unit, Microbiology and Virology Department, Fondazione IRCCS Policlinico San Matteo, 27100, Pavia, Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: Percivalle
authors.affiliation: Virology Unit; Fondazione Cà Granda Ospedale Maggiore, Policlinico, Milano, Italy.
authors.forename: Cristina
authors.initials: C
authors.lastname: Viganò
authors.affiliation: Virology Unit; Fondazione Cà Granda Ospedale Maggiore, Policlinico, Milano, Italy.
authors.forename: Teresa
authors.initials: T
authors.lastname: Nasta
authors.affiliation: Neonatology and Intensive Neonatal Care Unit, Fondazione Cà Granda Ospedale Maggiore, Policlinico, Milano, Italy.
authors.forename: Lorenza
authors.initials: L
authors.lastname: Pugni
authors.affiliation: Neonatology and Intensive Neonatal Care Unit, Fondazione Cà Granda Ospedale Maggiore, Policlinico, Milano, Italy.
authors.forename: Fabio
authors.initials: F
authors.lastname: Mosca
authors.affiliation: Neonatal Department, Neonatology and Intensive Neonatal Care, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy.
authors.forename: Mauro
authors.initials: M
authors.lastname: Stronati
authors.affiliation: Virology Unit; Fondazione Cà Granda Ospedale Maggiore, Policlinico, Milano, Italy.
authors.forename: Erminio
authors.initials: E
authors.lastname: Torresani
authors.affiliation: Molecular Virology Unit, Microbiology and Virology Department, Fondazione IRCCS Policlinico San Matteo, 27100, Pavia, Italy. Electronic address: f.baldanti@smatteo.pv.it.
authors.forename: Fausto
authors.initials: F
authors.lastname: Baldanti
mesh_terms: D006801:Humans; D007231:Infant, Newborn; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D012141:Respiratory Tract Infections / Q000175:diagnosis* / Q000821:virology; D012189:Retrospective Studies; D012680:Sensitivity and Specificity; D013997:Time Factors; D014777:Virus Diseases / Q000175:diagnosis* / Q000821:virology
keywords: FilmArray; Multiple PCR tests; Respiratory viruses