title: Evaluation of the HerpeSelect Express rapid test in the detection of herpes simplex virus type 2 antibodies in patients with genital ulcer disease.
abstract: BACKGROUND
  A rapid point-of-care test with high sensitivity and specificity is required in order to fulfill the need for early detection and screening of Herpes simplex virus type 2 (HSV-2) infection among patients with genital ulcer disease (GUD), for better management and control of virus transmission.
  
  
  METHODS
  The goal of this study is to evaluate the performance of the commercially available HerpeSelect Express rapid test in comparison with three ELISA assays: HerpeSelect ELISA, Kalon HSV-2 glycoprotein G2 assay, and monoclonal antibody blocking enzyme immunoassay, which was used as the gold standard for the detection of HSV-2 antibodies.
  
  
  RESULTS
  This study showed high sensitivity (ranging from 82.6 to 100%) and specificity (100%) of the HerpeSelect Express rapid test when compared to the three ELISA assays. The agreement between the HerpeSelect Express rapid test with the three ELISAs ranged from 93.3 to 100%.
  
  
  CONCLUSIONS
  The HerpeSelect Express rapid test has adequate sensitivity and specificity for confirming HSV-2 infection in patients with GUD, indicating its suitability for epidemiological studies.
authors.affiliation: Department of Dermatology, College of Medicine, Qassim University, Buraydah, Kingdom of Saudi Arabia.
authors.forename: Hani
authors.initials: H
authors.lastname: Al-Shobaili
authors.forename: Khaled M
authors.initials: KM
authors.lastname: Hassanein
authors.forename: Marwa Salah
authors.initials: MS
authors.lastname: Mostafa
authors.forename: Ali Saleh
authors.initials: AS
authors.lastname: Al Duways
mesh_terms: D000328:Adult; D000914:Antibodies, Viral / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006023:Glycoproteins / Q000276:immunology*; D006558:Herpes Genitalis / Q000097:blood / Q000150:complications / Q000821:virology; D018258:Herpesvirus 2, Human / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D018709:Statistics, Nonparametric; D014456:Ulcer / Q000097:blood / Q000150:complications / Q000821:virology; D014764:Viral Proteins / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D006023:Glycoproteins; C527357:US4 protein, human herpesvirus 2; D014764:Viral Proteins
keywords: HSV-2 antibodies; HerpeSelect ELISA; genital ulcer; herpes simplex virus 2; monoclonal antibody blocking EIA