title: Surface plasmon resonance technique for directly probing the interaction of DNA and graphene oxide and ultra-sensitive biosensing.
abstract: The binding of DNA with graphene oxide (GO) is important for applications in disease diagnosis, genetic screening, and drug discovery. The standard assay methods are mainly limited to indirect observation via fluorescence labeling. Here we report the use of surface plasmon resonance for direct sensing of DNA/GO binding. We show that this can be used for ultra-sensitive detection of single-stranded DNA (ssDNA). Furthermore, the results provide a more direct probe of DNA/GO binding abilities and confirm that hydrogen bonding plays a key role in the interaction between GO and ssDNA. This enables to a novel biosensor for highly sensitive and selective detection of ssDNA based on indirect competitive inhibition assay (ICIA). We report development of such a sensor with a linear dynamic range of 10(-14)-10(-6)M, a detection limit of 10fM and a high level of stability during repeated regeneration.
authors.affiliation: Key Laboratory of Automobile Materials of MOE and State Key Laboratory of Superhard Materials, Department of Materials Science, Jilin University, Changchun 130012, People׳s Republic of China.
authors.forename: Tianyu
authors.initials: T
authors.lastname: Xue
authors.affiliation: Key Laboratory of Automobile Materials of MOE and State Key Laboratory of Superhard Materials, Department of Materials Science, Jilin University, Changchun 130012, People׳s Republic of China. Electronic address: xqcui@jlu.edu.cn.
authors.forename: Xiaoqiang
authors.initials: X
authors.lastname: Cui
authors.affiliation: State Key Laboratory of Advanced Technologies for Comprehensive Utilization of Platinum Metals, Kunming 650106, People׳s Republic of China.
authors.forename: Weiming
authors.initials: W
authors.lastname: Guan
authors.affiliation: Key Laboratory of Automobile Materials of MOE and State Key Laboratory of Superhard Materials, Department of Materials Science, Jilin University, Changchun 130012, People׳s Republic of China.
authors.forename: Qiyu
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Key Laboratory of Automobile Materials of MOE and State Key Laboratory of Superhard Materials, Department of Materials Science, Jilin University, Changchun 130012, People׳s Republic of China.
authors.forename: Chang
authors.initials: C
authors.lastname: Liu
authors.affiliation: Key Laboratory of Automobile Materials of MOE and State Key Laboratory of Superhard Materials, Department of Materials Science, Jilin University, Changchun 130012, People׳s Republic of China.
authors.forename: Haitao
authors.initials: H
authors.lastname: Wang
authors.affiliation: Key Laboratory of Automobile Materials of MOE and State Key Laboratory of Superhard Materials, Department of Materials Science, Jilin University, Changchun 130012, People׳s Republic of China.
authors.forename: Kun
authors.initials: K
authors.lastname: Qi
authors.affiliation: Materials Science and Technology Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831-6056, United States.
authors.forename: D J
authors.initials: DJ
authors.lastname: Singh
authors.affiliation: Key Laboratory of Automobile Materials of MOE and State Key Laboratory of Superhard Materials, Department of Materials Science, Jilin University, Changchun 130012, People׳s Republic of China. Electronic address: wtzheng@jlu.edu.cn.
authors.forename: Weitao
authors.initials: W
authors.lastname: Zheng
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D004247:DNA / Q000032:analysis* / Q000737:chemistry*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006046:Gold / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D015336:Molecular Probe Techniques; D020349:Surface Plasmon Resonance / Q000295:instrumentation*
chemical_list: D006046:Gold; D006108:Graphite; D004247:DNA
keywords: Au nanoparticles; DNA; Graphene oxide; Hydrogen bond; Surface plasmon resonance