title: Reduced birth weight, cleft palate and preputial abnormalities in a cloned dog.
abstract: The aim of the present study was to report a novel developmental abnormality in a cloned dog. A fibroblast cell line was established from an 8-year-old male German shepherd dog. In vivo matured oocytes were retrieved from a large breed dog, and the nucleus was removed from each oocyte. A donor cell was injected into an enucleated oocyte, and the oocyte-cell couplet was fused electrically. After chemical activation, the resulting embryos were transferred into a naturally estrus-synchronized recipient dog, and two cloned pups were delivered by Cesarean section 60 days later. One cloned pup (Clone 1) was healthy, but the other (Clone 2) had a birth weight of only 320 g and cleft palate, failure of preputial closure at the ventral distal part, and persistent penile frenulum. Clone 2 was raised by stomach feeding until Day 40 after birth, where palatoplasty was performed. The abnormalities in external genitalia in Clone 2 resulted in persistent penile extrusion that was surgically corrected. This complex developmental abnormality has not been reported in dogs previously.
authors.forename: Min Jung
authors.initials: MJ
authors.lastname: Kim
authors.forename: Hyun Ju
authors.initials: HJ
authors.lastname: Oh
authors.forename: Geon A
authors.initials: GA
authors.lastname: Kim
authors.forename: Young Kwang
authors.initials: YK
authors.lastname: Jo
authors.forename: Jin
authors.initials: J
authors.lastname: Choi
authors.forename: Hye Jin
authors.initials: HJ
authors.lastname: Kim
authors.forename: Hee Yeon
authors.initials: HY
authors.lastname: Choi
authors.forename: Hyun Wook
authors.initials: HW
authors.lastname: Kim
authors.forename: Min Cheol
authors.initials: MC
authors.lastname: Choi
authors.affiliation: Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 151-742, Republic of Korea. bclee@snu.ac.kr.
authors.forename: Byeong Chun
authors.initials: BC
authors.lastname: Lee
mesh_terms: D000818:Animals; D001724:Birth Weight; D002460:Cell Line; D002585:Cesarean Section / Q000662:veterinary; D002972:Cleft Palate / Q000235:genetics / Q000662:veterinary*; D019976:Cloning, Organism; D004285:Dogs / Q000002:abnormalities* / Q000254:growth & development*; D005260:Female; D008297:Male; D010413:Penis / Q000002:abnormalities* / Q000254:growth & development / Q000601:surgery; D011247:Pregnancy; D016896:Treatment Outcome