title: Thermomechanical effects of co-solute on the structure formation of bovine serum albumin.
abstract: The effect of glucose syrup on the structural properties of bovine serum albumin has been addressed in preparations from low to high solids. Fifteen percent protein was mixed with the co-solute at concentrations up to 65% and subjected to thermal treatment to examine the changes in phase and state transitions. Thermomechanics were the working protocol being carried out with micro differential scanning calorimetry and small deformation dynamic oscillation. Results argue that protein molecules have been extensively stabilised by the addition of a co-solute, recorded via a delayed thermal denaturation. Further, increasing the glucose syrup enhanced polymer-polymer interactions leading to stronger networks following thermal denaturation of the globular protein. Condensed BSA/glucose syrup mixtures, i.e. at 80% solids, were cooled at subzero temperatures to exhibit a considerable state of vitrification. Molecular relaxation phenomena were successfully followed using theoretical concepts from synthetic polymer research to yield the mechanical glass transition temperature.
authors.affiliation: School of Applied Sciences, RMIT University, City Campus, Melbourne, VIC 3001, Australia.
authors.forename: Paul
authors.initials: P
authors.lastname: George
authors.affiliation: Animal, Food and Health Sciences, CSIRO, Werribee, VIC 3030, Australia.
authors.forename: Leif
authors.initials: L
authors.lastname: Lundin
authors.affiliation: School of Applied Sciences, RMIT University, City Campus, Melbourne, VIC 3001, Australia. Electronic address: stefan.kasapis@rmit.edu.au.
authors.forename: Stefan
authors.initials: S
authors.lastname: Kasapis
mesh_terms: D002151:Calorimetry; D002152:Calorimetry, Differential Scanning / Q000379:methods*; D005947:Glucose / Q000737:chemistry*; D012212:Rheology; D012710:Serum Albumin, Bovine / Q000737:chemistry*
chemical_list: D012710:Serum Albumin, Bovine; D005947:Glucose
keywords: Bovine serum albumin; Calorimetry; Glass transition; Glucose syrup; Rheology