title: Characterization, kinetic, and thermodynamic studies of marine pectinase from Bacillus subtilis.
abstract: Characterization, kinetic and thermodynamic parameters of purified pectinase from Bacillus subtilis, isolated from a marine sediment sample collected from Chinchani beach at Tarapore, India, were studied. Marine pectinase produced under submerged growth conditions was purified by ammonium sulfate precipitation followed by gel filtration chromatography using DEAE cellulose. Partial characterization of the marine pectinase was carried out in terms of effect of pH, temperature, substrate concentration, and metal ions. It was found that pectinase from marine B. subtilis showed maximal activity in alkaline buffer at pH 9.0 and at 40°C. It was also found that metal ions, namely, Mn(2+) and Fe(2+), stimulate pectinase activity. Marine pectinase followed Michaelis-Menten kinetics. The kinetics and thermodynamic parameters of the purified marine pectinase from B. subtilis were studied as the characterization of the enzyme is vital for its use in industrial processes.
authors.affiliation: a Department of Fibres and Textile Processing Technology , Institute of Chemical Technology, Matunga , Mumbai , India.
authors.forename: Manasi
authors.initials: M
authors.lastname: Joshi
authors.forename: Madhura
authors.initials: M
authors.lastname: Nerurkar
authors.forename: Ravindra
authors.initials: R
authors.lastname: Adivarekar
mesh_terms: D001412:Bacillus subtilis / Q000201:enzymology*; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008970:Molecular Weight; D011096:Polygalacturonase / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D013816:Thermodynamics
chemical_list: D011096:Polygalacturonase
keywords: Bacillus subtilis; kinetics; marine; pectinase; thermodynamic parameters