title: Chemicals and lemon essential oil effect on Alicyclobacillus acidoterrestris viability.
abstract: Alicyclobacillus acidoterrestris is considered to be one of the important target microorganisms in the quality control of acidic canned foods. There is an urgent need to develop a suitable method for inhibiting or controlling the germination and outgrowth of A.acidoterrestris in acidic drinks. The aim of this work was to evaluate the chemicals used in the lemon industry (sodium benzoate, potassium sorbate), and lemon essential oil as a natural compound, against a strain of A.acidoterrestris in MEB medium and in lemon juice concentrate. The results pointed out that sodium benzoate (500-1000-2000 ppm) and lemon essential oil (0.08-0.12-0.16%) completely inhibited the germination of A. acidoterrestris spores in MEB medium and LJC for 11 days. Potassium sorbate (600-1200 ppm) was more effective to inhibit the growth of the microbial target in lemon juice than in MEB medium. The effect of sodium benzoate, potassium sorbate and essential oil was sporostatic in MEB and LJC as they did not affect spore viability.
authors.affiliation: Instituto de Biotecnología, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, Tucumán, Argentina.
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Maldonado
authors.affiliation: Instituto de Biotecnología, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, Tucumán, Argentina.
authors.forename: Marina Paola
authors.initials: MP
authors.lastname: Aban
authors.affiliation: Instituto de Biotecnología, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, Tucumán, Argentina.
authors.forename: Antonio Roberto
authors.initials: AR
authors.lastname: Navarro
mesh_terms: D056450:Alicyclobacillus / Q000187:drug effects* / Q000502:physiology*; D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002957:Citrus / Q000737:chemistry*; D003470:Culture Media / Q000737:chemistry; D050296:Microbial Viability / Q000187:drug effects*; D009822:Oils, Volatile / Q000302:isolation & purification / Q000494:pharmacology*; D020160:Sodium Benzoate / Q000494:pharmacology; D013171:Spores, Bacterial / Q000187:drug effects / Q000502:physiology
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media; D009822:Oils, Volatile; D020160:Sodium Benzoate
keywords: Alicyclobacillus acidoterrestris; essential oils; potassium sorbate; sodium benzoate