title: Ménage à trois: post-transcriptional control of the key enzyme for cell envelope synthesis by a base-pairing small RNA, an RNase adaptor protein, and a small RNA mimic.
abstract: In Escherichia coli, small RNAs GlmY and GlmZ feedback control synthesis of glucosamine-6-phosphate (GlcN6P) synthase GlmS, a key enzyme required for synthesis of the cell envelope. Both small RNAs are highly similar, but only GlmZ is able to activate the glmS mRNA by base-pairing. Abundance of GlmZ is controlled at the level of decay by RNase adaptor protein RapZ. RapZ binds and targets GlmZ to degradation by RNase E via protein-protein interaction. GlmY activates glmS indirectly by protecting GlmZ from degradation. Upon GlcN6P depletion, GlmY accumulates and sequesters RapZ in an RNA mimicry mechanism, thus acting as an anti-adaptor. As a result, this regulatory circuit adjusts synthesis of GlmS to the level of its enzymatic product, thereby mediating GlcN6P homeostasis. The interplay of RNase adaptor proteins and anti-adaptors provides an elegant means how globally acting RNases can be re-programmed to cleave a specific transcript in response to a cognate stimulus.
authors.affiliation: Max F. Perutz Laboratories; Department of Microbiology; Immunobiology and Genetics; Center of Molecular Biology; University of Vienna; Vienna, Austria.
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Göpel
authors.affiliation: Max F. Perutz Laboratories; Department of Microbiology; Immunobiology and Genetics; Center of Molecular Biology; University of Vienna; Vienna, Austria.
authors.forename: Muna A
authors.initials: MA
authors.lastname: Khan
authors.affiliation: Max F. Perutz Laboratories; Department of Microbiology; Immunobiology and Genetics; Center of Molecular Biology; University of Vienna; Vienna, Austria.
authors.forename: Boris
authors.initials: B
authors.lastname: Görke
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D020029:Base Pairing; D002473:Cell Wall / Q000235:genetics* / Q000378:metabolism; D004722:Endoribonucleases / Q000378:metabolism; D004789:Enzyme Activation; D015964:Gene Expression Regulation, Bacterial; D005944:Glucosamine / Q000031:analogs & derivatives / Q000378:metabolism; D019298:Glucose-6-Phosphate / Q000031:analogs & derivatives / Q000378:metabolism; D006090:Gram-Negative Bacteria / Q000235:genetics / Q000378:metabolism; D006094:Gram-Positive Bacteria / Q000235:genetics / Q000378:metabolism; D054884:Host-Pathogen Interactions; D018716:Molecular Mimicry; D009876:Operon / Q000235:genetics; D026723:Polyadenylation; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D012323:RNA Processing, Post-Transcriptional; D020871:RNA Stability; D012329:RNA, Bacterial / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D016337:RNA, Catalytic / Q000235:genetics / Q000378:metabolism; D058727:RNA, Small Untranslated / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012260:Ribonucleases / Q000378:metabolism; D005945:Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing)
chemical_list: D001426:Bacterial Proteins; D004722:Endoribonucleases; D005944:Glucosamine; D019298:Glucose-6-Phosphate; D012329:RNA, Bacterial; D016337:RNA, Catalytic; D058727:RNA, Small Untranslated; D012260:Ribonucleases; C001293:glucosamine 6-phosphate; C022537:ribonuclease E; C000731191:component S, glutamate mutase protein, Bacteria; D005945:Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing)
keywords: RNA mimicry; RNase E adaptor protein RapZ (YhbJ); decoy small RNA GlmY; glucosamine-6-phosphate synthase GlmS; small RNA GlmZ; two-component system GlrK/GlrR (QseE/QseF)