title: Analysis of sitosteryl oleate esters in phytosterols esters enriched foods by HPLC-ESI-MS(2.).
abstract: Phytosteryl esters (PE)-enriched spreads are marketed for eating and cooking purposes. Temperature and also light exposure are the major factors leading to the formation of PE oxides in food matrix. In this study a high-speed HPLC-MS(2) method was developed to analyze the major PE present in PE-enriched spreads: sitosteryl oleate (SO) and its oxidation products, by using synthesized compounds as standards. This analytical method was used to quantify seven SO oxides formed in PE-enriched spreads after heating at different temperatures for varying time periods and after prolonged exposure to sunlight. Quantification of remaining native SO was also performed after these different treatments. It was found that under specific heating conditions the decrease of the SO amount was much more important compared to the formation of SO oxides showing that many other products are formed. In contrast to heating, sunlight radiation did not result in the degradation of SO and very few oxides were formed.
authors.affiliation: IPHC, UMR 7178, Equipe de Chimie Analytique des Molécules BioActives-Université de Strasbourg, Faculté de Pharmacie, 74 route du Rhin, 67400 Illkirch, France. Electronic address: diane.julien-david@unistra.fr.
authors.forename: Diane
authors.initials: D
authors.lastname: Julien-David
authors.affiliation: IPHC, UMR 7178, Equipe de Chimie Analytique des Molécules BioActives-Université de Strasbourg, Faculté de Pharmacie, 74 route du Rhin, 67400 Illkirch, France.
authors.forename: Minjie
authors.initials: M
authors.lastname: Zhao
authors.affiliation: Institut de Chimie, UMR 7177, Laboratoire de Chimie Organique Synthétique-Université de Strasbourg, 1 rue de Blaise Pascal, BP 296/R8, 67008 Strasbourg cedex, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Geoffroy
authors.affiliation: Institut de Chimie, UMR 7177, Laboratoire de Chimie Organique Synthétique-Université de Strasbourg, 1 rue de Blaise Pascal, BP 296/R8, 67008 Strasbourg cedex, France.
authors.forename: Michel
authors.initials: M
authors.lastname: Miesch
authors.affiliation: IRCAD, INSERM U682, Université de Strasbourg, Faculté de Médecine, 1 place de l'hôpital, BP406, 67091 Strasbourg cedex, France.
authors.forename: Francis
authors.initials: F
authors.lastname: Raul
authors.affiliation: Aérial, Parc d'innovation, rue Laurent Fries-BP40443, 67412 Illkirch cedex, France.
authors.forename: Dalal
authors.initials: D
authors.lastname: Aoude-Werner
authors.affiliation: IPHC, UMR 7178, Equipe de Chimie Analytique des Molécules BioActives-Université de Strasbourg, Faculté de Pharmacie, 74 route du Rhin, 67400 Illkirch, France.
authors.forename: Saïd
authors.initials: S
authors.lastname: Ennahar
authors.affiliation: IPHC, UMR 7178, Equipe de Chimie Analytique des Molécules BioActives-Université de Strasbourg, Faculté de Pharmacie, 74 route du Rhin, 67400 Illkirch, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Marchioni
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004952:Esters; D057230:Limit of Detection; D015203:Reproducibility of Results; D012855:Sitosterols / Q000032:analysis* / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D004952:Esters; D012855:Sitosterols
keywords: Enriched foods; HPLC–MS(2); Oxidation; Phytosterols; Phytosteryl esters