title: Protective effects of combined intervention with adenovirus vector mediated IL-10 and IGF-1 genes on endogenous islet β cells in nonobese diabetes mice with onset of type 1 diabetes mellitus.
abstract: OBJECTIVE
  To investigate the protective effects of combined intervention with adenovirus vector mediated interleukin 10 (IL-10) and insulin-like growth factor 1 (IGF-1) genes on islet β cells in nonobese diabetes (NOD) mice with type 1 diabetes mellitus (T1D) at early stage.
  
  
  METHODS
  Twenty-four female NOD mice at onset of diabetes and aged 17-20 weeks old were randomly divided into four groups. Mouse 1, 2 and 3 groups were intraperitoneally injected 0.1 ml of Ad-mIGF-1, Ad-mIL-10, and combined Ad-mIGF-1 and Ad-mIL-10, respectively. Mouse 4 group were used as diabetes control. In addition, six age- and sex-matched non-diabetic NOD mice were intraperitoneally injected 0.1 ml of PBS and assigned 5 group as normal controls. All mice were weekly monitored for body weight, urine glucose and blood glycose, and sacrificed 3 weeks after injection. Their serum levels of IL-10, IGF-1, IFN-γ, IL-4 and C-peptide were measured and the degree of insulitis and the local expression of IGF-1 and IL-10 gene were observed.
  
  
  RESULTS
  1) IL-10 and IGF-1 levels in serum and pancreas were enhanced in 1, 2, and 3 groups; 2) serum INF-γ level was decreased while serum IL-10 and IL-4 levels were increased in 1, 2 and 3 groups, and these alterations were more significant in 3 group than 1 and 2 groups (P<0.01); 3) C-peptide level was not enhanced in 1 group, but significantly increased in 2 and 3 groups, and these increases were more significant in the latter (P<0.01); 4) Three weeks later, the body mass of mice in 2 and 3 groups decreased significantly (P<0.05).
  
  
  CONCLUSIONS
  The administration of adenovirus vector mediated IL-10 and/or IGF-1 gene showed limited immune regulatory and protective effects on islet β-cells in NOD mice with T1D at early stage, and no significant reduction in insulitis, blood glucose and body weight.
authors.affiliation: Department of Pediatrics, Affiliated Hospital of Medical College Qingdao University, Qingdao, Shandong, China.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Pediatrics, People's Hospital of RiZhao, Rizhao, Shandong, China.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Pediatrics, Affiliated Hospital of Medical College Qingdao University, Qingdao, Shandong, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Pediatrics, Affiliated Hospital of Medical College Qingdao University, Qingdao, Shandong, China.
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Lin
authors.affiliation: Department of Medical Center, Qingdao Sanatorium of Shandong Province, Qingdao, Shandong, China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Cheng
authors.affiliation: Department of Pediatrics, Affiliated Hospital of Medical College Qingdao University, Qingdao, Shandong, China.
authors.forename: Tang
authors.initials: T
authors.lastname: Li
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight / Q000235:genetics; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000235:genetics* / Q000473:pathology / Q000628:therapy; D005260:Female; D005786:Gene Expression Regulation / Q000235:genetics; D015316:Genetic Therapy; D005822:Genetic Vectors / Q000235:genetics*; D007334:Insulin-Like Growth Factor I / Q000235:genetics*; D016753:Interleukin-10 / Q000097:blood / Q000235:genetics*; D007515:Islets of Langerhans / Q000378:metabolism*; D051379:Mice; D016688:Mice, Inbred NOD
chemical_list: D001786:Blood Glucose; D016753:Interleukin-10; D007334:Insulin-Like Growth Factor I