title: Alveolar bone resorption induced by CD4+CD45RB high-density T-cell transfer in immunocompromised mice.
abstract: BACKGROUND
  The aims of this study are to determine whether the antigen-inexperienced (naive, CD45RB high-density) T-cell (CD4(+)CD45RB(High) T-cell) transfer model is associated with alveolar bone resorption, to elucidate the local osteogenic/adipogenic potential of alveolar bone marrow stromal cells (ABCs) from T-cell-transferred animals, and to investigate the systemic osteogenic potential by transplanting human periodontal ligament stem cells (hPDLSCs) into these animals.
  
  
  METHODS
  CD4(+)CD45RB(High) and CD4(+)CD45RB(Low) (antigen-experienced [memory, CD45RB low-density]) T cells were sorted and transferred into severe combined immunodeficiency (SCID) mice to induce inflammatory bowel disease-like syndrome (n = 8). hPDLSCs were transplanted into T-cell-transferred SCID mice to examine ectopic cementum formation 8 weeks after T-cell transfer. The mandibles and tibias of these mice were retrieved for microcomputed tomography (micro-CT), histomorphometric analysis, and isolation of ABCs 16 weeks after T-cell transfer. The in vitro osteogenic and adipogenic potentials of the ABCs were evaluated.
  
  
  RESULTS
  Histologic and micro-CT analysis revealed that the transfer of CD4(+)CD45RB(High) T-cell subset was sufficient for alveolar bone resorption and affected the osteogenic/adipogenic potential of ABCs. Furthermore, it was found that CD4(+)CD45RB(High) T-cell-transferred animals have decreased systemic osteogenic potential, as evidenced using the in vivo ectopic hPDLSC transplantation model.
  
  
  CONCLUSIONS
  CD4(+)CD45RB(High) T-cell transfer induced both alveolar bone resorption and reduced systemic osteogenic potential, with a concomitant downregulation of the osteogenic potential of ABCs.
authors.affiliation: Department of Periodontology, Research Institute for Periodontal Regeneration, Yonsei University College of Dentistry, Seoul, Korea.
authors.forename: Jung-Chul
authors.initials: JC
authors.lastname: Park
authors.forename: Bo-Kyoung
authors.initials: BK
authors.lastname: Kim
authors.forename: Im-Hee
authors.initials: IH
authors.lastname: Jung
authors.forename: Eunyoung
authors.initials: E
authors.lastname: Choi
authors.forename: Chang-Sung
authors.initials: CS
authors.lastname: Kim
mesh_terms: D050156:Adipogenesis / Q000276:immunology; D019264:Adoptive Transfer / Q000379:methods*; D000328:Adult; D016301:Alveolar Bone Loss / Q000276:immunology* / Q000473:pathology; D000539:Alveolar Process / Q000473:pathology; D000818:Animals; D015519:Bone Density / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000637:transplantation; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000276:immunology; D002478:Cells, Cultured; D025021:Cementogenesis / Q000276:immunology; D005260:Female; D006801:Humans; D016867:Immunocompromised Host; D015212:Inflammatory Bowel Diseases / Q000276:immunology; D017493:Leukocyte Common Antigens / Q000276:immunology*; D008297:Male; D008334:Mandible / Q000276:immunology / Q000473:pathology; D059630:Mesenchymal Stem Cells / Q000276:immunology; D051379:Mice; D008813:Mice, Inbred ICR; D016513:Mice, SCID; D010012:Osteogenesis / Q000276:immunology; D010513:Periodontal Ligament / Q000166:cytology / Q000276:immunology; D033581:Stem Cell Transplantation / Q000379:methods; D013977:Tibia / Q000276:immunology / Q000473:pathology; D055114:X-Ray Microtomography / Q000379:methods; D055815:Young Adult
chemical_list: D017493:Leukocyte Common Antigens
keywords: Alveolar bone loss; CD4-positive T-lymphocytes; inflammatory bowel diseases