title: Immunogenicity of a recombinant Mycobacterium smegmatis vaccine expressing the fusion protein CMX in cattle from Goiás State, Brazil.
abstract: This study aimed to evaluate the immunogenicity of a recombinant Mycobacterium smegmatis vaccine expressing the CMX fusion protein composed of immunodominant epitopes Ag85C, MPT51 and HspX of Mycobacterium tuberculosis, which are important mycobacteria virulence factors. A group of Nelore heifers that were 10 to 12 months of age and negative for the tuberculin skin test (TST) were immunized with four doses of the recombinant vaccine mc(2)-CMX (M. smegmatis-Ag85C-MPT51-HspX) during a period of one year. Before each immunization, blood was collected to obtain sera for antibody analysis. Serological analysis demonstrated that mc(2)-CMX was able to induce a humoral response with increased levels of specific IgG antibodies against CMX, despite minimum antibody levels being detected for individual Ag85C, MPT51 or HspX recombinant antigens. However, there was no significant increase in specific CD4(+) IFN-γ-positive T cells. Lymphadenomegaly was observed in superficial cervical lymph nodes adjacent to the site of vaccination among mc(2)-CMX-vaccinated bovines, and the histopathological analysis demonstrated follicular hyperplasia without inflammatory infiltrate or granuloma formation. Animals remained negative for the TST until the end of the experiments, showing no cross-reactivity with the recombinant vaccine and tuberculin proteins. We discuss the potential of mc(2)-CMX to induce an immune response in cattle.
authors.affiliation: Tropical Pathology and Public Health Institute, Federal University of Goiás, Rua 235 esquina com 1a Avenida, Setor Universitário, Goiânia, Goiás, CEP 74605-050, Brazil.
authors.forename: Duanne
authors.initials: D
authors.lastname: Alves Da Silva
authors.forename: Marcos Antônio Rocha
authors.initials: MA
authors.lastname: Cavalcanti
authors.forename: Fábio
authors.initials: F
authors.lastname: Muniz De Oliveira
authors.forename: Monalisa Martins
authors.initials: MM
authors.lastname: Trentini
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: Junqueira-Kipnis
authors.forename: André
authors.initials: A
authors.lastname: Kipnis
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology*; D001938:Brazil / Q000453:epidemiology; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000517:prevention & control*; D005260:Female; D016056:Immunodominant Epitopes / Q000276:immunology; D009165:Mycobacterium Infections, Nontuberculous / Q000517:prevention & control / Q000662:veterinary*; D020102:Mycobacterium smegmatis / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000276:immunology
chemical_list: D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D016056:Immunodominant Epitopes; D014614:Vaccines, Synthetic