title: Epigallocatechin-3-gallate ameliorates seawater aspiration-induced acute lung injury via regulating inflammatory cytokines and inhibiting JAK/STAT1 pathway in rats.
abstract: Signal transducers and activators of transcriptions 1 (STAT1) play an important role in the inflammation process of acute lung injury (ALI). Epigallocatechin-3-gallate (EGCG) exhibits a specific and strong anti-STAT1 activity. Therefore, our study is to explore whether EGCG pretreatment can ameliorate seawater aspiration-induced ALI and its possible mechanisms. We detected the arterial partial pressure of oxygen, lung wet/dry weight ratios, protein content in bronchoalveolar lavage fluid, and the histopathologic and ultrastructure staining of the lung. The levels of IL-1, TNF-α, and IL-10 and the total and the phosphorylated protein level of STAT1, JAK1, and JAK2 were assessed in vitro and in vivo. The results showed that EGCG pretreatment significantly improved hypoxemia and histopathologic changes, alleviated pulmonary edema and lung vascular leak, reduced the production of TNF-α and IL-1, and increased the production of IL-10 in seawater aspiration-induced ALI rats. EGCG also prevented the seawater aspiration-induced increase of TNF-α and IL-1 and decrease of IL-10 in NR8383 cell line. Moreover, EGCG pretreatment reduced the total and the phosphorylated protein level of STAT1 in vivo and in vitro and reduced the phosphorylated protein level of JAK1 and JAK2. The present study demonstrates that EGCG ameliorates seawater aspiration-induced ALI via regulating inflammatory cytokines and inhibiting JAK/STAT1 pathway in rats.
authors.affiliation: Department of Pulmonary Diseases, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Pathology and Pathophysiology, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Mingqing
authors.initials: M
authors.lastname: Dong
authors.affiliation: Department of Pulmonary Diseases, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
authors.forename: Liyan
authors.initials: L
authors.lastname: Bo
authors.affiliation: Department of Pulmonary Diseases, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
authors.forename: Congcong
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Pulmonary Diseases, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
authors.forename: Qingqing
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Department of Pulmonary Diseases, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Pulmonary Diseases, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
authors.forename: Lijie
authors.initials: L
authors.lastname: Ma
authors.affiliation: Department of Pulmonary Diseases, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Xie
authors.affiliation: Department of Pulmonary Diseases, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
authors.forename: Enqing
authors.initials: E
authors.lastname: Fu
authors.affiliation: Department of Pulmonary Diseases, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
authors.forename: Deguang
authors.initials: D
authors.lastname: Mu
authors.affiliation: Department of Pulmonary Diseases, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Pan
authors.affiliation: Department of Pulmonary Diseases, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
authors.forename: Faguang
authors.initials: F
authors.lastname: Jin
authors.affiliation: Department of Pathology and Pathophysiology, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Zhichao
authors.initials: Z
authors.lastname: Li
mesh_terms: D055371:Acute Lung Injury / Q000188:drug therapy* / Q000503:physiopathology; D000818:Animals; D062186:Arterial Pressure; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D016207:Cytokines / Q000378:metabolism; D007375:Interleukin-1 / Q000378:metabolism; D016753:Interleukin-10 / Q000378:metabolism; D053613:Janus Kinase 1 / Q000378:metabolism; D053614:Janus Kinase 2 / Q000378:metabolism; D008264:Macrophages / Q000166:cytology; D008297:Male; D010100:Oxygen / Q000737:chemistry; D010313:Partial Pressure; D010766:Phosphorylation; D051381:Rats; D017207:Rats, Sprague-Dawley; D053120:Respiratory Aspiration / Q000188:drug therapy* / Q000503:physiopathology; D050794:STAT1 Transcription Factor / Q000378:metabolism; D012623:Seawater; D015398:Signal Transduction / Q000187:drug effects*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D016207:Cytokines; D007375:Interleukin-1; D050794:STAT1 Transcription Factor; C494081:Stat1 protein, rat; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D002392:Catechin; C045651:epigallocatechin gallate; C507906:Jak1 protein, rat; C507927:Jak2 protein, rat; D053613:Janus Kinase 1; D053614:Janus Kinase 2; D010100:Oxygen