title: A novel protein extracted from foxtail millet bran displays anti-carcinogenic effects in human colon cancer cells.
abstract: Millet is an important cereal food and exhibits multiple biological activities, including immunodulatory, antioxidant, antifungal and anti-hyperglycemia effects. Herein, we describe a novel 35kDa protein with anti-cancer properties, named FMBP, which was extracted and purified from foxtail millet bran by cell-based screening. FMBP is highly homologous to peroxidase as revealed by mass spectrometry and gene sequencing analysis. Furthermore, in vivo anti-tumor results implicated that the novel protein FMBP had the ability to suppress xenografted tumor growth in nude mice. Mechanistically, FMBP is able to suppress colon cancer cell growth through induction of G1 phase arrest and also causes a loss of mitochondrial transmembrane potential which results in caspase-dependent apoptosis in colon cancer cells. Notably, FMBP has much lower toxicity in normal colon epithelial cells. Taken together, FMBP has targeted anti-colon cancer effects and may serve as a therapeutic agent against colon cancer.
authors.affiliation: Key Laboratory of Chemical Biology and Molecular Engineering of National Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan 030006, China; Department of Biology, Taiyuan Normal University, Taiyuan 030031, China.
authors.forename: Shuhua
authors.initials: S
authors.lastname: Shan
authors.affiliation: Key Laboratory of Chemical Biology and Molecular Engineering of National Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan 030006, China.
authors.forename: Zongwei
authors.initials: Z
authors.lastname: Li
authors.affiliation: Cell and Developmental Biology, University of Dundee, Dundee, Scotland DD1 5EH, UK.
authors.forename: Ian P
authors.initials: IP
authors.lastname: Newton
authors.affiliation: Key Laboratory of Chemical Biology and Molecular Engineering of National Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan 030006, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Chemical Biology and Molecular Engineering of National Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan 030006, China; Colleague of Life Science, Zhejiang Chinese Medical University, Hangzhou 310053, China. Electronic address: lzy@sxu.edu.cn.
authors.forename: Zhuoyu
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Chemistry and Biochemistry, University of Massachusetts Dartmouth, Dartmouth, MA 02747, USA.
authors.forename: Maolin
authors.initials: M
authors.lastname: Guo
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D017209:Apoptosis; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000188:drug therapy*; D004847:Epithelial Cells; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D010940:Plant Proteins / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D032983:Setaria Plant / Q000737:chemistry*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D010940:Plant Proteins
keywords: Anti-cancer properties; Colon cancer; FMBP; Foxtail millet bran