title: Novel μ-membrane module for online determination of the free fatty acid content in the dispersed phase of oil-in-water emulsions.
abstract: Monitoring the dispersed phase of an oil-in-water (O-W) emulsion by means of Fourier transform infrared (FTIR) spectroscopy is a challenging task, restricted to the continuous phase that is in contact with the FTIR probe. Nonetheless, real-time measurement and kinetic analysis by FTIR, including analysis of the dispersed, often non-polar phase containing substrates and/or products, is desirable. Enzymatic hydrolysis of sunflower oil was performed in an O-W emulsion. After separation of the oil phase by use of a newly developed μ-membrane module, infrared spectra were collected using an attenuated total reflectance (ATR) cell. Different chemometric models were calibrated using the partial least squares (PLS) algorithm. Online application of a chemometric model based on the FTIR spectra enabled real-time monitoring of free fatty acid concentrations in the oil phase.
authors.affiliation: Institute of Technical Biocatalysis, Hamburg University of Technology, Denickestraße 15, 21075, Hamburg, Germany.
authors.forename: Dennis
authors.initials: D
authors.lastname: Kaufhold
authors.forename: Janosch
authors.initials: J
authors.lastname: Fagaschewski
authors.forename: Daniel
authors.initials: D
authors.lastname: Sellin
authors.forename: Simon
authors.initials: S
authors.lastname: Strompen
authors.forename: Andreas
authors.initials: A
authors.lastname: Liese
authors.forename: Lutz
authors.initials: L
authors.lastname: Hilterhaus
mesh_terms: D000465:Algorithms; D004655:Emulsions; D005230:Fatty Acids, Nonesterified / Q000032:analysis*; D006868:Hydrolysis; D007700:Kinetics; D016018:Least-Squares Analysis; D008049:Lipase / Q000378:metabolism; D008567:Membranes, Artificial; D009862:Online Systems; D010938:Plant Oils / Q000032:analysis*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D000074242:Sunflower Oil
chemical_list: D004655:Emulsions; D005230:Fatty Acids, Nonesterified; D008567:Membranes, Artificial; D010938:Plant Oils; D000074242:Sunflower Oil; D008049:Lipase