title: In vivo antinociceptive and anti-inflammatory activities of umbelliferone isolated from Potentilla evestita.
abstract: This study was designed to evaluate the antinociceptive and anti-inflammatory activities of a compound, umbelliferone, isolated from the chloroform fraction of Potentilla evestita in animal models. When tested against acetic acid-induced noxious stimulus, it significantly prolonged pain threshold and provided 38.38% and 60.95% reduction in abdominal constriction at 5 and 10 mg/kg i.p., respectively. Post-umbelliferone injection evoked significant dose-dependent reduction in noxious stimulation with 33.65% and 58.89% pain attenuation at 5 and 10 mg/kg i.p., respectively, in the initial phase. In the late phase, it illustrated more dominant effect with 37.65% and 63.79% blockade of painful sensation. Similarly, it exhibited significant anti-inflammatory activity during various assessment times (1-5 h) with 46.28% and 66.13% amelioration after 4th of administration against induced oedema. In conclusion, umbelliferone possessed strong antinociceptive and anti-inflammatory activities by inhibiting both peripheral and centrally acting pain mediators.
authors.affiliation: a Institute of Chemical Sciences, University of Peshawar , Peshawar - 25120 , KPK , Pakistan.
authors.forename: Abdur
authors.initials: A
authors.lastname: Rauf
authors.forename: Rehan
authors.initials: R
authors.lastname: Khan
authors.forename: Haroon
authors.initials: H
authors.lastname: Khan
authors.forename: Samreen
authors.initials: S
authors.lastname: Pervez
authors.forename: Abdul Saboor
authors.initials: AS
authors.lastname: Pirzada
mesh_terms: D000700:Analgesics / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D002351:Carrageenan / Q000494:pharmacology; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy; D008297:Male; D015394:Molecular Structure; D010146:Pain / Q000188:drug therapy; D031987:Potentilla / Q000737:chemistry*; D014468:Umbelliferones / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*
chemical_list: D000700:Analgesics; D000893:Anti-Inflammatory Agents; D014468:Umbelliferones; C031477:7-hydroxycoumarin; D002351:Carrageenan
keywords: Potentilla evestita; antinociceptive and anti-inflammatory activity; umbelliferone