title: Syncytin-1 and its receptor is present in human gametes.
abstract: To determine whether the true fusogen Syncytin-1 and its receptor (ASCT-2) is present in human gametes using qRT-PCR, immunoblotting and immunofluorescence.
  
  
  METHODS
  Donated oocytes and spermatozoa, originating from a fertility center in tertiary referral university hospital, underwent qRT-PCR, immunoblotting and immunofluorescence analyzes.
  
  
  RESULTS
  Quantitative RT-PCR of sperm samples from sperm donors showed that syncytin-1 is present in all samples, however, protein levels varied between donors. Syncytin-1 immunoreactivity predominates in the sperm head and around the equatorial segment. The receptor ASCT-2 is expressed in the acrosomal region and in the sperm tail. Moreover, ASCT-2, but not syncytin-1, is expressed in oocytes and the mRNA level increases with increasing maturity of the oocytes.
  
  
  CONCLUSIONS
  Syncytin and its receptor are present in human gametes and localization and temporal appearance is consistent with a possible role in fusion between oocyte and sperm.
authors.affiliation: Faculty of Life Science, University of Copenhagen, Copenhagen, Denmark.
authors.forename: B
authors.initials: B
authors.lastname: Bjerregaard
authors.forename: J G
authors.initials: JG
authors.lastname: Lemmen
authors.forename: M R
authors.initials: MR
authors.lastname: Petersen
authors.forename: E
authors.initials: E
authors.lastname: Østrup
authors.forename: L H
authors.initials: LH
authors.lastname: Iversen
authors.forename: K
authors.initials: K
authors.lastname: Almstrup
authors.forename: L-I
authors.initials: LI
authors.lastname: Larsson
authors.forename: S
authors.initials: S
authors.lastname: Ziebe
mesh_terms: D000328:Adult; D026962:Amino Acid Transport System ASC / Q000235:genetics* / Q000378:metabolism; D005260:Female; D005306:Fertilization / Q000502:physiology; D018507:Gene Expression Regulation, Developmental; D015686:Gene Products, env / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008297:Male; D009865:Oocytes / Q000502:physiology*; D011257:Pregnancy Proteins / Q000235:genetics* / Q000378:metabolism; D013077:Sperm Head / Q000502:physiology; D013094:Spermatozoa / Q000502:physiology*
chemical_list: D026962:Amino Acid Transport System ASC; D015686:Gene Products, env; D011257:Pregnancy Proteins; C498848:SLC1A4 protein, human; C405335:syncytin