title: Long-pulsed luminescence for the measurement of dissolved oxygen.
abstract: Thin-film luminescent sensors were used to measure dissolved oxygen in picoliter volumes for the purpose of monitoring single-cell oxygen consumption rates, and that work served as the motivation for the development of the method described here. A few different platinum porphyrin sensor materials were examined, with all measurements conducted microscopically. By employing convolution theory to understand observed responses, including an unexpected red luminescent emission from an optic, we developed a new, rapid method for the determination of exponential decay lifetime. This new method of long-pulsed luminescence offers substantially improved signal-to-noise ratios for detected signals as long as self-illumination sources are carefully controlled in the experimental set-up.
authors.affiliation: University of Washington, Department of Chemistry, Seattle, WA 98195 USA.
authors.forename: Noel S
authors.initials: NS
authors.lastname: Fitzgerald
authors.affiliation: University of Washington, Department of Chemistry, Seattle, WA 98195 USA.
authors.forename: Lloyd W
authors.initials: LW
authors.lastname: Burgess
authors.affiliation: University of Washington, Department of Chemistry, Seattle, WA 98195 USA.
authors.forename: Jeffrey C-Y
authors.initials: JC
authors.lastname: Yang
authors.affiliation: University of Washington, Department of Chemistry, Seattle, WA 98195 USA.
authors.forename: Prince J
authors.initials: PJ
authors.lastname: Kim
authors.affiliation: University of Washington, Department of Chemistry, Seattle, WA 98195 USA.
authors.forename: Sei-Hum
authors.initials: SH
authors.lastname: Jang
authors.affiliation: University of Washington, Department of Chemistry, Seattle, WA 98195 USA.
authors.forename: Alex K-Y
authors.initials: AK
authors.lastname: Jen
mesh_terms: D008163:Luminescent Measurements / Q000379:methods*; D008665:Metalloporphyrins / Q000737:chemistry; D010100:Oxygen / Q000032:analysis*; D059629:Signal-To-Noise Ratio
chemical_list: D008665:Metalloporphyrins; D010100:Oxygen