title: Exogenous expression of marine lectins DlFBL and SpRBL induces cancer cell apoptosis possibly through PRMT5-E2F-1 pathway.
abstract: Lectins are widely existed in marine bioresources, and some purified marine lectins were found toxic to cancer cells. In this report, genes encoding Dicentrarchus labrax fucose-binding lectin (DlFBL) and Strongylocentrotus purpuratus rhamnose-binding lectin (SpRBL) were inserted into an adenovirus vector to form Ad.FLAG-DlFBL and Ad.FLAG-SpRBL, which elicited significant in vitro suppressive effect on a variety of cancer cells. Anti-apoptosis factors Bcl-2 and XIAP were determined to be downregulated by Ad.FLAG-DlFBL and Ad.FLAG-SpRBL. Subcellular localization studies showed that DlFBL but not SpRBL widely distributed in membrane systems. Both DlFBL and SpRBL were shown associated with protein arginine methyltransferase 5 (PRMT5), and PRMT5-E2F-1 pathway was suggested to be responsible for the DlFBL and SpRBL induced apoptosis. Further investigations revealed that PRMT5 acted as a common binding target for various exogenous lectin and non-lectin proteins, suggesting a role of PRMT5 as a barrier for foreign gene invasion. The cellular response to exogenous lectins may provide insights into a novel way for cancer gene therapy.
authors.affiliation: 1] College of life sciences, Zhejiang Sci-Tech University, Hangzhou, Zhejiang, China [2].
authors.forename: Liqin
authors.initials: L
authors.lastname: Wu
authors.affiliation: 1] College of life sciences, Zhejiang Sci-Tech University, Hangzhou, Zhejiang, China [2].
authors.forename: Xinyan
authors.initials: X
authors.lastname: Yang
authors.affiliation: College of life sciences, Zhejiang Sci-Tech University, Hangzhou, Zhejiang, China.
authors.forename: Xuemei
authors.initials: X
authors.lastname: Duan
authors.affiliation: College of life sciences, Zhejiang Sci-Tech University, Hangzhou, Zhejiang, China.
authors.forename: Lianzhen
authors.initials: L
authors.lastname: Cui
authors.affiliation: College of life sciences, Zhejiang Sci-Tech University, Hangzhou, Zhejiang, China.
authors.forename: Gongchu
authors.initials: G
authors.lastname: Li
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D017209:Apoptosis / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D050687:E2F1 Transcription Factor / Q000378:metabolism*; D029941:Fish Proteins / Q000235:genetics* / Q000378:metabolism; D005399:Fishes / Q000235:genetics; D015870:Gene Expression; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D037102:Lectins / Q000235:genetics* / Q000378:metabolism; D011485:Protein Binding; D021381:Protein Transport; D011484:Protein-Arginine N-Methyltransferases / Q000378:metabolism*; D015398:Signal Transduction; D047270:Strongylocentrotus purpuratus / Q000235:genetics
chemical_list: D050687:E2F1 Transcription Factor; D029941:Fish Proteins; D037102:Lectins; C027112:fucose-binding lectin; C400529:PRMT5 protein, human; D011484:Protein-Arginine N-Methyltransferases