title: Metal oxide affinity chromatography platform-polydopamine coupled functional two-dimensional titania graphene nanohybrid for phosphoproteome research.
abstract: In this work, a facile route was initially developed for preparation of a novel metal oxide affinity chromatography (MOAC) material by grafting titania nanoparticles on polydopamine (PD)-coated graphene (denoted as G@PD@TiO2). In the first step, self-assemble polymerization of dopamine on graphene was performed in basic solution at room temperature, which not only offered the coupling linker between titania and graphene but also improved the hydrophilicity and biological compatibility of the nanohybrids. Thereafter, the titania nanoparticles were grafted on the surface of the PD-coated graphene via a simple hydrothermal treatment. The as-prepared G@PD@TiO2 nanohybrids exhibited high sensitivity (detection limit of 5 fmol) and high selectivity for phosphopeptides at a low molar ratio of phosphopeptides/nonphosphopeptides (1:1000). Moreover, the as-prepared nanohybrids were also investigated for enrichment of phosphopeptides from real biological samples (human serum and mouse brain). A total number of 556 phosphorylation sites were identified from the digest of mouse brain proteins, showing great potential in the practical application.
authors.affiliation: Department of Chemistry, Fudan University , Shanghai 200433, China.
authors.forename: Yinghua
authors.initials: Y
authors.lastname: Yan
authors.forename: Xueni
authors.initials: X
authors.lastname: Sun
authors.forename: Chunhui
authors.initials: C
authors.lastname: Deng
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Xiangmin
authors.initials: X
authors.lastname: Zhang
mesh_terms: D002846:Chromatography, Affinity / Q000379:methods*; D007211:Indoles / Q000737:chemistry*; D008670:Metals / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D010087:Oxides / Q000737:chemistry; D010750:Phosphoproteins / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D020543:Proteome; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D007211:Indoles; D008670:Metals; D010087:Oxides; D010750:Phosphoproteins; D011108:Polymers; D020543:Proteome; C568283:polydopamine