title: Effect of hesperidin and neohesperidin from bittersweet orange (Citrus aurantium var. bigaradia) peel on indomethacin-induced peptic ulcers in rats.
abstract: Hesperidin and neohesperidin are the major flavanones isolated from bittersweet orange. It was recently reported that they have potent anti-inflammatory effects in various inflammatory models. In the present study, the effects of hesperidin and neohesperidin on indomethacin-induced ulcers in rats and the underlying mechanisms were investigated. Gastric ulcers were induced in rats with a single dose of indomethacin. The effects of pretreatment with hesperidin and neohesperidin were assessed in comparison with omeprazole as reference standard. Ulcer index, gene expression of gastric cyclooxygenase-2 (COX-2), tumor necrosis factor alpha (TNF-α), lipid peroxidation product, malondialdhyde (MDA), and reduced glutathione (GSH) content in stomach were measured. Furthermore, gross and histopathological examination was performed. Our results indicated that both hesperidin and neohesperidin significantly aggravated gastric damage caused by indomethacin administration as evidenced by increased ulcer index and histopathological changes of stomach.
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Zagazig University, Zagazig 44519, Egypt.
authors.forename: Dalia I
authors.initials: DI
authors.lastname: Hamdan
authors.affiliation: Department of Pharmacology and Toxicology, Faculty of Pharmacy, Zagazig University, Zagazig 44519, Egypt.
authors.forename: Mona F
authors.initials: MF
authors.lastname: Mahmoud
authors.affiliation: Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, ImNeuenheimer Feld 364, 69120 Heidelberg, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Wink
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Zagazig University, Zagazig 44519, Egypt. Electronic address: assemels2002@yahoo.co.uk.
authors.forename: Assem M
authors.initials: AM
authors.lastname: El-Shazly
mesh_terms: D000818:Animals; D002957:Citrus; D051546:Cyclooxygenase 2 / Q000235:genetics; D005638:Fruit; D005753:Gastric Mucosa / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D006569:Hesperidin / Q000009:adverse effects / Q000031:analogs & derivatives* / Q000302:isolation & purification; D007213:Indomethacin; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D010437:Peptic Ulcer / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology; D017208:Rats, Wistar; D013270:Stomach / Q000187:drug effects / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
chemical_list: D014409:Tumor Necrosis Factor-alpha; D008315:Malondialdehyde; D006569:Hesperidin; D051546:Cyclooxygenase 2; C489691:Ptgs2 protein, rat; D005978:Glutathione; C546526:neohesperidin; D007213:Indomethacin
keywords: Citrus×aurantium var. bigaradia; Gastric ulcer; Hesperidin; LC-ESI/MS; Neohesperidin