title: Construction of a high-coverage bacterial artificial chromosome library and comprehensive genetic linkage map of yellowtail Seriola quinqueradiata.
abstract: BACKGROUND
  Japanese amberjack/yellowtail (Seriola quinqueradiata) is a commonly cultured marine fish in Japan. For cost effective fish production, a breeding program that increases commercially important traits is one of the major solutions. In selective breeding, information of genetic markers is useful and sufficient to identify individuals carrying advantageous traits but if the aim is to determine the genetic basis of the trait, large insert genomic DNA libraries are essential. In this study, toward prospective understanding of genetic basis of several economically important traits, we constructed a high-coverage bacterial artificial chromosome (BAC) library, obtained sequences from the BAC-end, and constructed comprehensive female and male linkage maps of yellowtail using Simple Sequence Repeat (SSR) markers developed from the BAC-end sequences and a yellowtail genomic library.
  
  
  RESULTS
  The total insert length of the BAC library we constructed here was estimated to be approximately 11 Gb and hence 16-times larger than the yellowtail genome. Sequencing of the BAC-ends showed a low fraction of repetitive sequences comparable to that in Tetraodon and fugu. A total of 837 SSR markers developed here were distributed among 24 linkage groups spanning 1,026.70 and 1,057.83 cM with an average interval of 4.96 and 4.32 cM in female and male map respectively without any segregation distortion. Oxford grids suggested conserved synteny between yellowtail and stickleback.
  
  
  CONCLUSIONS
  In addition to characteristics of yellowtail genome such as low repetitive sequences and conserved synteny with stickleback, our genomic and genetic resources constructed and revealed here will be powerful tools for the yellowtail breeding program and also for studies regarding the genetic basis of traits.
authors.forename: Kanako
authors.initials: K
authors.lastname: Fuji
authors.forename: Takashi
authors.initials: T
authors.lastname: Koyama
authors.forename: Wataru
authors.initials: W
authors.lastname: Kai
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kubota
authors.forename: Kazunori
authors.initials: K
authors.lastname: Yoshida
authors.forename: Akiyuki
authors.initials: A
authors.lastname: Ozaki
authors.forename: Jun-ya
authors.initials: JY
authors.lastname: Aoki
authors.forename: Yumi
authors.initials: Y
authors.lastname: Kawabata
authors.forename: Kazuo
authors.initials: K
authors.lastname: Araki
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Tsuzaki
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Okamoto
authors.affiliation: Faculty of Marine Science, Tokyo University of Marine Science and Technology, 4-5-7, Konan, Minato-ku, Tokyo 108-8477, Japan. takashis@kaiyodai.ac.jp.
authors.forename: Takashi
authors.initials: T
authors.lastname: Sakamoto
mesh_terms: D000818:Animals; D001947:Breeding; D002874:Chromosome Mapping; D022202:Chromosomes, Artificial, Bacterial; D005260:Female; D005399:Fishes / Q000235:genetics*; D008040:Genetic Linkage; D005819:Genetic Markers; D059646:Genome Size; D015698:Genomic Library; D008297:Male; D018895:Microsatellite Repeats; D019655:Quantitative Trait, Heritable; D026801:Synteny
chemical_list: D005819:Genetic Markers