title: A simple and effective coumarin-based fluorescent probe for cysteine.
abstract: Acrylic acid 3-acetyl-2-oxo-2 H-chromen-7-yl ester (ACA) was rationally designed and synthesized as a simple and effective fluorescent probe for sensing cysteine with high selectivity and naked-eye detection. The probe can detect cysteine by fluorescence spectrometry with a detection limit of 0.657 μM and can be used with calf serum and in live cell imaging. The conjugate addition/cyclization sequence mechanism of the reaction between ACA and cysteine was conﬁrmed by ESI-MS and fluorescence spectra.
authors.affiliation: Institute of Organic Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, P.R. China.
authors.forename: Xi
authors.initials: X
authors.lastname: Dai
authors.affiliation: Institute of Developmental Biology, School of Life Science, Shandong University, Jinan 250100, P.R. China.
authors.forename: Qing-Hua
authors.initials: QH
authors.lastname: Wu
authors.affiliation: Institute of Developmental Biology, School of Life Science, Shandong University, Jinan 250100, P.R. China.
authors.forename: Peng-Chong
authors.initials: PC
authors.lastname: Wang
authors.affiliation: Institute of Organic Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, P.R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Tian
authors.affiliation: Institute of Organic Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, P.R. China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Institute of Organic Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, P.R. China.
authors.forename: Sheng-Qing
authors.initials: SQ
authors.lastname: Wang
authors.affiliation: Institute of Developmental Biology, School of Life Science, Shandong University, Jinan 250100, P.R. China. Electronic address: miaojy@sdu.edu.cn.
authors.forename: Jun-Ying
authors.initials: JY
authors.lastname: Miao
authors.affiliation: Institute of Organic Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, P.R. China. Electronic address: bxzhao@sdu.edu.cn.
authors.forename: Bao-Xiang
authors.initials: BX
authors.lastname: Zhao
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods; D002417:Cattle; D003374:Coumarins / Q000737:chemistry*; D003545:Cysteine / Q000032:analysis* / Q000097:blood*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D057230:Limit of Detection; D061848:Optical Imaging / Q000379:methods; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D003374:Coumarins; D005456:Fluorescent Dyes; C030123:coumarin; D003545:Cysteine
keywords: Calf serum; Cell imaging; Conjugate addition; Coumarin; Cysteine; Fluorescent probe