title: The murine intravaginal HSV-2 challenge model for investigation of DNA vaccines.
abstract: DNA vaccines have been licensed in veterinary medicine and have promise for humans. This format is relatively immunogenic in mice and guinea pigs, the two principle HSV-2 animal models, permitting rapid assessment of vectors, antigens, adjuvants, and delivery systems. Limitations include the relatively poor immunogenicity of naked DNA in humans and the profound differences in HSV-2 pathogenesis between host species. Herein, we detail lessons learned over the last few years investigating candidate DNA vaccines in the progesterone-primed female mouse vaginal model of HSV-2 infection as a guide to investigators in the field.
authors.affiliation: Department of Medicine, University of Washington, 750 Republican Street, Room E651, Mail Stop 35806, Seattle, WA, 98195, USA.
authors.forename: Joshua O
authors.initials: JO
authors.lastname: Marshak
authors.forename: Lichun
authors.initials: L
authors.lastname: Dong
authors.forename: David M
authors.initials: DM
authors.lastname: Koelle
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D004195:Disease Models, Animal; D006168:Guinea Pigs; D018258:Herpesvirus 2, Human / Q000235:genetics / Q000276:immunology*; D006801:Humans; D051379:Mice; D008967:Molecular Biology / Q000379:methods*; D011374:Progesterone / Q000378:metabolism; D019444:Vaccines, DNA / Q000235:genetics / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D019444:Vaccines, DNA; D014759:Viral Envelope Proteins; D011374:Progesterone