title: Computational tools for rational protein engineering of aldolases.
abstract: In this mini-review we describe the different strategies for rational protein engineering and summarize the computational tools available. Computational tools can either be used to design focused libraries, to predict sequence-function relationships or for structure-based molecular modelling. This also includes de novo design of enzymes. Examples for protein engineering of aldolases and transaldolases are given in the second part of the mini-review.
authors.affiliation: Institute of Technical Biochemistry, University of Stuttgart, Allmandring 31, 70569 Stuttgart, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Widmann
authors.affiliation: Institute of Technical Biochemistry, University of Stuttgart, Allmandring 31, 70569 Stuttgart, Germany.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Pleiss
authors.affiliation: Institute of Microbiology, University of Stuttgart, Allmandring 31, 70569 Stuttgart, Germany.
authors.forename: Anne K
authors.initials: AK
authors.lastname: Samland
keywords: biocatalysis; de novo design; protein engineering; stereoselectivity; substrate specificity; transaldolase