title: Purification of bovine serum paraoxonase and its immobilization on Eupergit C 250 L by covalent attachment.
abstract: Serum paraoxonase (PON1) is a high-density lipoprotein (HDL)-associated enzyme that protects lipoproteins, both low-density lipoprotein (LDL) and HDL, against oxidation, and is considered as an antioxidative/anti-inflammatory component of HDL. In this study, PON1 was purified from bovine serum by ammonium sulfate precipitation and hydrophobic interaction chromatography on sepharose-4B-l-tyrosine-1-napthylamine. It was then immobilized on an unmodified Eupergit® C 250 L support. The immobilized PON1 retained a high catalytic activity and showed increased thermal stability compared to the native enzyme.
authors.affiliation: Department of Chemistry, Science and Art Faculty, Balikesir University , Cagıs-Kampus, Balikesir , Turkey and.
authors.forename: Murat
authors.initials: M
authors.lastname: Sayın
authors.forename: Ozen Ozensoy
authors.initials: OO
authors.lastname: Guler
mesh_terms: D000645:Ammonium Sulfate / Q000737:chemistry; D000818:Animals; D043303:Aryldialkylphosphatase / Q000737:chemistry / Q000302:isolation & purification*; D001798:Blood Proteins / Q000737:chemistry / Q000302:isolation & purification*; D002417:Cattle; D011232:Chemical Precipitation; D002845:Chromatography / Q000379:methods; D004795:Enzyme Stability; D057927:Hydrophobic and Hydrophilic Interactions; D055767:Immobilized Proteins / Q000737:chemistry / Q000302:isolation & purification*; D007700:Kinetics; D011108:Polymers / Q000737:chemistry
chemical_list: D001798:Blood Proteins; D055767:Immobilized Proteins; D011108:Polymers; C065843:Eupergit C; D043303:Aryldialkylphosphatase; D000645:Ammonium Sulfate
keywords: Eupergit C 250 L; immobilization; paraoxonase; purification