title: Enhancing bright-field image of microorganisms by local plasmon of Ag nanoparticle array.
abstract: Inspecting biological cells with bright-field light microscopy often engenders a challenge, owing to their optical transparency. We show that imaging contrast can be greatly enhanced as yeast cells are placed on a silver nanoparticle array. Its near- and far-field traits, revealed by electrodynamic simulations, illustrate that the enhancement is attributed to the sensitivity of its plasmonic characteristics to the attached cells. This study demonstrates that the silver nanoparticle array can serve as the agent for concurrently enhancing Raman scattering and imaging contrast of microorganisms for identification and examination.
authors.forename: Hui-Hsin
authors.initials: HH
authors.lastname: Hsiao
authors.forename: Po-Chun
authors.initials: PC
authors.lastname: Yeh
authors.forename: Huai-Hsien
authors.initials: HH
authors.lastname: Wang
authors.forename: Tian-You
authors.initials: TY
authors.lastname: Cheng
authors.forename: Hung-Chun
authors.initials: HC
authors.lastname: Chang
authors.forename: Yuh-Lin
authors.initials: YL
authors.lastname: Wang
authors.forename: Juen-Kai
authors.initials: JK
authors.lastname: Wang
mesh_terms: D053768:Metal Nanoparticles; D008853:Microscopy / Q000295:instrumentation*; D012441:Saccharomyces cerevisiae / Q000166:cytology*; D012834:Silver / Q000737:chemistry*; D020349:Surface Plasmon Resonance / Q000295:instrumentation*
chemical_list: D012834:Silver