title: Comparative protein composition analysis of goat milk produced by the Alpine and Saanen breeds in northeastern Brazil and related antibacterial activities.
abstract: The protein composition of goat milk differs between goat breeds and could present regional trends. The aim of this study was to comparatively analyze the protein composition of goat milk produced by the Alpine and Saanen breeds in northeastern Brazil and to evaluate the antibacterial activity of its protein fractions. SDS-PAGE, 2-DE electrophoresis and RP-HPLC analyses revealed the absence of αs1-casein in the milk of both breeds and no differences between the αs2-casein, β-casein, β-lactoglobulin and α-lactalbumin profiles. The amounts of soluble proteins and β-casein hydrolysis residues were higher in Saanen milk. Only the protein fraction containing the largest amounts of casein (F60-90%) inhibited bacterial growth, with MIC values between 50 and 100 mg/mL. This study describe for the first time three important points about the goat milk protein of two Brazilian goat breeders: absence of α-s1 casein in the protein profile, differences between the milk protein composition produced by goats of Alpine and Saanen breeders and antibacterial activity of unbroken proteins (casein-rich fraction) present in these milk.
authors.affiliation: Department of Food Engineering, Technology Center, Federal University of Paraíba, João Pessoa, Paraíba, Brazil.
authors.forename: Whyara Karoline Almeida da
authors.initials: WK
authors.lastname: Costa
authors.affiliation: Department of Nutrition, Health Sciences Center, Federal University of Paraíba, João Pessoa, Paraíba, Brazil.
authors.forename: Evandro Leite de
authors.initials: EL
authors.lastname: Souza
authors.affiliation: Department of Food Engineering, Technology Center, Federal University of Paraíba, João Pessoa, Paraíba, Brazil.
authors.forename: Edvaldo Mesquita
authors.initials: EM
authors.lastname: Beltrão-Filho
authors.affiliation: Department of Cellular Biology, Exact and Nature Science Center, Federal University of Paraíba, João Pessoa, Paraíba, Brazil.
authors.forename: Gracy Kelly Vieira
authors.initials: GK
authors.lastname: Vasconcelos
authors.affiliation: Department of Cellular Biology, Exact and Nature Science Center, Federal University of Paraíba, João Pessoa, Paraíba, Brazil.
authors.forename: Tatiane
authors.initials: T
authors.lastname: Santi-Gadelha
authors.affiliation: Department of Cellular Biology, Exact and Nature Science Center, Federal University of Paraíba, João Pessoa, Paraíba, Brazil.
authors.forename: Carlos Alberto
authors.initials: CA
authors.lastname: de Almeida Gadelha
authors.affiliation: Center of Biochemical and Proteomic Analyses, Pos-Graduation of Biotechnology and Genomic Sciences, Catholic University of Brasilia, Distrito Federal, Brazil.
authors.forename: Octavio Luiz
authors.initials: OL
authors.lastname: Franco
authors.affiliation: Department of Food Engineering, Technology Center, Federal University of Paraíba, João Pessoa, Paraíba, Brazil.
authors.forename: Marciane
authors.initials: M
authors.lastname: Magnani
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000187:drug effects / Q000254:growth & development; D001938:Brazil; D001947:Breeding; D002364:Caseins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development; D005260:Female; D006041:Goats; D006868:Hydrolysis; D007768:Lactalbumin / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D008826:Microbial Sensitivity Tests; D008892:Milk / Q000737:chemistry*; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000254:growth & development; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development
chemical_list: D000900:Anti-Bacterial Agents; D002364:Caseins; D007768:Lactalbumin