title: Improved plasma cholesterol efflux capacity from human macrophages in patients with hyperalphalipoproteinemia.
abstract: OBJECTIVE
  CETP or HL deficiencies lead to a marked increase in HDL-C levels however the atheroprotective effect of this phenotype, in particular the ability of HDL particles to remove cholesterol from human macrophages, remains to be determined.
  
  
  METHODS
  We measured cholesterol efflux from human THP-1 macrophages to total plasma or to isolated HDL subfractions in patients with HALP carrying molecular defect in either the CETP or LIPC gene.
  
  
  RESULTS
  We demonstrate that HALP is associated with an increased plasma cholesterol efflux capacity from human macrophages. This observation is primarily related to a stimulation of both SR-BI and ABCA1 dependent efflux pathways as a result of quantitative elevation in HDL2 and enhanced intrinsic capacity of HDL3 subspecies, respectively.
  
  
  CONCLUSIONS
  HDL particles from HALP patients with molecular defect within either CETP or LIPC gene are not dysfunctional and are efficient to stimulate cholesterol efflux from human macrophages.
authors.affiliation: INSERM-UPMC UMR1166, Hôpital de la Pitié, Paris, France; Laboratoire de Biochimie, Faculté de Pharmacie et Pôle Technologie Santé, Université Saint-Joseph, Beirut, Lebanon.
authors.forename: Petra
authors.initials: P
authors.lastname: El Khoury
authors.affiliation: Hormonal and Metabolic Disorders Research Unit and Endocrinology and Metabolism Unit, Department of Medecine, Faculty of Medicine, Chulalongkorn University and King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Patumwan, Bangkok, Thailand.
authors.forename: Wanee
authors.initials: W
authors.lastname: Plengpanich
authors.affiliation: INSERM-UPMC UMR1166, Hôpital de la Pitié, Paris, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Frisdal
authors.affiliation: INSERM-UPMC UMR1166, Hôpital de la Pitié, Paris, France; ICAN-Institute of CardioMetabolism and Nutrition, Hôpital de la Pitié, Paris, France.
authors.forename: Wilfried
authors.initials: W
authors.lastname: Le Goff
authors.affiliation: Hormonal and Metabolic Disorders Research Unit and Endocrinology and Metabolism Unit, Department of Medecine, Faculty of Medicine, Chulalongkorn University and King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Patumwan, Bangkok, Thailand.
authors.forename: Weerapan
authors.initials: W
authors.lastname: Khovidhunkit
authors.affiliation: INSERM-UPMC UMR1166, Hôpital de la Pitié, Paris, France; ICAN-Institute of CardioMetabolism and Nutrition, Hôpital de la Pitié, Paris, France. Electronic address: maryse.guerin@upmc.fr.
authors.forename: Maryse
authors.initials: M
authors.lastname: Guerin
mesh_terms: D000328:Adult; D000368:Aged; D002784:Cholesterol / Q000097:blood* / Q000378:metabolism; D053480:Cholesterol Ester Transfer Proteins / Q000097:blood / Q000172:deficiency* / Q000378:metabolism; D005260:Female; D006801:Humans; D008052:Lipid Metabolism, Inborn Errors / Q000097:blood* / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D008875:Middle Aged
chemical_list: D053480:Cholesterol Ester Transfer Proteins; D002784:Cholesterol
keywords: CETP; Cholesterol efflux; Genetic deficiency; HDL; Hepatic lipase; Macrophage; Reverse cholesterol transport