title: The Cytotoxic Effects of Low Intensity Visible and Infrared Light on Human Breast Cancer (MCF7) cells.
abstract: A concept of using low intensity light therapy (LILT) as an alternative approach to cancer treatment is at early stages of development; while the therapeutic effects of LILT as a non-invasive treatment modality for localized joint and soft tissue wound healing are widely corroborated. The LEDs-based exposure system was designed and constructed to irradiate the selected cancer and normal cells and evaluate the biological effects induced by light exposures in visible and infrared light range. In this study, human breast cancer (MCF7) cells and human epidermal melanocytes (HEM) cells (control) were exposed to selected far infrared light (3400nm, 3600nm, 3800nm, 3900nm, 4100nm and 4300nm) and visible and near infrared wavelengths (466nm, 585nm, 626nm, 810nm, 850nm and 950nm). The optical intensities of LEDs used for exposures were in the range of 15µW to 30µW. Cellular morphological changes of exposed and sham-exposed cells were evaluated using light microscopy. The cytotoxic effects of these low intensity light exposures on human cancer and normal cell lines were quantitatively determined by Lactate dehydrogenase (LDH) cytotoxic activity and PrestoBlue™ cell viability assays. Findings reveal that far-infrared exposures were able to reduce cell viability of MCF7 cells as measured by increased LDH release activity and PrestoBlue™ assays. Further investigation of the effects of light irradiation on different types of cancer cells, study of possible signaling pathways affected by electromagnetic radiation (EMR) and in vivo experimentation are required in order to draw a firm conclusion about the efficacy of low intensity light as an alternative non-invasive cancer treatment.
authors.affiliation: School of Electrical and Computer Engineering, RMIT University, Melbourne, Australia.
authors.forename: P
authors.initials: P
authors.lastname: Peidaee
authors.affiliation: Department of Biotechnology and Environmental Biology, School of Applied Sciences, RMIT University, Australia.
authors.forename: N
authors.initials: N
authors.lastname: Almansour
authors.affiliation: Centre for Advanced Materials and Industrial Chemistry, School of Applied Sciences, RMIT University, Australia ; Health Innovation Research Institute, RMIT University, Australia.
authors.forename: R
authors.initials: R
authors.lastname: Shukla
authors.affiliation: School of Electrical and Computer Engineering, RMIT University, Melbourne, Australia ; Health Innovation Research Institute, RMIT University, Australia.
authors.forename: E
authors.initials: E
authors.lastname: Pirogova
keywords: Electromagnetic Radiation (EMR); Far Infrared wavelength exposure; Lactate dehydrogenase (LDH) Assay; Light microscopy; PrestoBlue™ Assay; Visible light exposure