title: Identification of carotenoids from the extremely halophilic archaeon Haloarcula japonica.
abstract: The carotenoids produced by extremely halophilic archaeon Haloarcula japonica were extracted and identified by their chemical, chromatographic, and spectroscopic characteristics (UV-Vis and mass spectrometry). The composition (mol%) was 68.1% bacterioruberin, 22.5% monoanhydrobacterioruberin, 9.3% bisanhydrobacterioruberin, <0.1% isopentenyldehydrorhodopin, and trace amounts of lycopene and phytoene. The in vitro scavenging capacity of a carotenoid, bacterioruberin, extracted from Haloarcula japonica cells against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals was evaluated. The antioxidant capacity of bacterioruberin was much higher than that of β -carotene.
authors.affiliation: Department of Bioengineering, Tokyo Institute of Technology Yokohama, Japan.
authors.forename: Rie
authors.initials: R
authors.lastname: Yatsunami
authors.affiliation: Department of Bioengineering, Tokyo Institute of Technology Yokohama, Japan.
authors.forename: Ai
authors.initials: A
authors.lastname: Ando
authors.affiliation: Department of Bioengineering, Tokyo Institute of Technology Yokohama, Japan.
authors.forename: Ying
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Biology, Nippon Medical School Kawasaki, Japan.
authors.forename: Shinichi
authors.initials: S
authors.lastname: Takaichi
authors.affiliation: Department of Bioengineering, Tokyo Institute of Technology Yokohama, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kohno
authors.affiliation: Department of Bioengineering, Tokyo Institute of Technology Yokohama, Japan.
authors.forename: Yuriko
authors.initials: Y
authors.lastname: Matsumura
authors.affiliation: Department of Bioengineering, Tokyo Institute of Technology Yokohama, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ikeda
authors.affiliation: Department of Bioengineering, Tokyo Institute of Technology Yokohama, Japan.
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Fukui
authors.affiliation: Department of Biotechnology and Chemistry, Kinki University Hiroshima, Japan.
authors.forename: Kaoru
authors.initials: K
authors.lastname: Nakasone
authors.affiliation: Biotechnology Field, National Institute of Technology and Evaluation Tokyo, Japan.
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Fujita
authors.affiliation: Biotechnology Field, National Institute of Technology and Evaluation Tokyo, Japan.
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Sekine
authors.affiliation: Department of Applied Bioscience, Toyo University Gunma, Japan.
authors.forename: Tomonori
authors.initials: T
authors.lastname: Takashina
authors.affiliation: Department of Bioengineering, Tokyo Institute of Technology Yokohama, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Nakamura
keywords: C50 carotenoid; Haloarcula japonica; antioxidant capacity; bacterioruberin; extremely halophilic archaeon