title: Identification of proteins of altered abundance in oil palm infected with Ganoderma boninense.
abstract: Basal stem rot is a common disease that affects oil palm, causing loss of yield and finally killing the trees. The disease, caused by fungus Ganoderma boninense, devastates thousands of hectares of oil palm plantings in Southeast Asia every year. In the present study, root proteins of healthy oil palm seedlings, and those infected with G. boninense, were analyzed by 2-dimensional gel electrophoresis (2-DE). When the 2-DE profiles were analyzed for proteins, which exhibit consistent significant change of abundance upon infection with G. boninense, 21 passed our screening criteria. Subsequent analyses by mass spectrometry and database search identified caffeoyl-CoA O-methyltransferase, caffeic acid O-methyltransferase, enolase, fructokinase, cysteine synthase, malate dehydrogenase, and ATP synthase as among proteins of which abundances were markedly altered.
authors.affiliation: Agro-Biotechnology Malaysia Institutes, c/o MARDI Headquarters, Serdang 43400, Selangor, Malaysia. yusmin_y@um.edu.my.
authors.forename: Jameel R
authors.initials: JR
authors.lastname: Al-Obaidi
authors.affiliation: Centre for Foundation Studies in Science, University of Malaya, Kuala Lumpur 50603, Malaysia. yasmin@um.edu.my.
authors.forename: Yusmin
authors.initials: Y
authors.lastname: Mohd-Yusuf
authors.affiliation: Department of Molecular Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia. hanani@um.edu.my.
authors.forename: Nurhanani
authors.initials: N
authors.lastname: Razali
authors.affiliation: University of Malaya Centre for Proteomics Research (UMCPR), University of Malaya, Kuala Lumpur 50603, Malaysia. jaime_jacklyn@um.edu.my.
authors.forename: Jaime Jacqueline
authors.initials: JJ
authors.lastname: Jayapalan
authors.affiliation: Plant Protection Unit, Sime Darby Sdn Bhd, Km 10, Jalan Banting Kelanang, P.O. Box 207, Banting 42100, Selangor, Malaysia. tchinchong@yahoo.com.
authors.forename: Chin-Chong
authors.initials: CC
authors.lastname: Tey
authors.affiliation: Plant Protection Unit, Sime Darby Sdn Bhd, Km 10, Jalan Banting Kelanang, P.O. Box 207, Banting 42100, Selangor, Malaysia. nanniiez82@gmail.com.
authors.forename: Normahnani
authors.initials: N
authors.lastname: Md-Noh
authors.affiliation: Department of Molecular Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia. jaime_jacklyn@um.edu.my.
authors.forename: Sarni Mat
authors.initials: SM
authors.lastname: Junit
authors.affiliation: Centre for Research in Biotechnology for Agriculture, University of Malaya, Kuala Lumpur 50603, Malaysia. yasmin@um.edu.my.
authors.forename: Rofina Yasmin
authors.initials: RY
authors.lastname: Othman
authors.affiliation: Department of Molecular Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia. onnhashim@um.edu.my.
authors.forename: Onn Haji
authors.initials: OH
authors.lastname: Hashim
mesh_terms: D028023:Arecaceae / Q000378:metabolism* / Q000382:microbiology*; D003547:Cysteine Synthase / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D005631:Fructokinases / Q000378:metabolism; D032902:Ganoderma / Q000502:physiology*; D054884:Host-Pathogen Interactions; D008291:Malate Dehydrogenase / Q000378:metabolism; D008780:Methyltransferases / Q000378:metabolism; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D010935:Plant Diseases / Q000382:microbiology; D010940:Plant Proteins / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism / Q000382:microbiology; D040901:Proteomics / Q000379:methods; D006180:Proton-Translocating ATPases / Q000378:metabolism; D053719:Tandem Mass Spectrometry
chemical_list: D010940:Plant Proteins; D008291:Malate Dehydrogenase; D008780:Methyltransferases; C059825:caffeoyl-CoA O-methyltransferase; C019608:caffeate O-methyltransferase; D003547:Cysteine Synthase; D005631:Fructokinases; C046173:fructokinase; D006180:Proton-Translocating ATPases; D010751:Phosphopyruvate Hydratase