title: CIAPIN1 gene silencing enhances chemosensitivity in a drug-resistant animal model in vivo.
abstract: Overexpression of cytokine-induced apoptosis inhibitor 1 (CIAPIN1) contributes to multidrug resistance (MDR) in breast cancer. This study aimed to evaluate the potential of CIAPIN1 gene silencing by RNA interference (RNAi) as a treatment for drug-resistant breast cancer and to investigate the effect of CIAPIN1 on the drug resistance of breast cancer in vivo. We used lentivirus-vector-based RNAi to knock down CIAPIN1 in nude mice bearing MDR breast cancer tumors and found that lentivirus-vector-mediated silencing of CIAPIN1 could efficiently and significantly inhibit tumor growth when combined with chemotherapy in vivo. Furthermore, Western blot analysis showed that both CIAPIN1 and P-glycoprotein expression were efficiently downregulated, and P53 was upregulated, after RNAi. Therefore, we concluded that lentivirus-vector-mediated RNAi targeting of CIAPIN1 is a potential approach to reverse MDR of breast cancer. In addition, CIAPIN1 may participate in MDR of breast cancer by regulating P-glycoprotein and P53 expression.
authors.affiliation: Department of Oncology, The Second Affiliated Hospital, Harbin Medical University, Harbin, China.
authors.forename: X M
authors.initials: XM
authors.lastname: Wang
authors.affiliation: Department of Oncology, The Second Affiliated Hospital, Harbin Medical University, Harbin, China.
authors.forename: S J
authors.initials: SJ
authors.lastname: Gao
authors.affiliation: Department of Oncology, The Second Affiliated Hospital, Harbin Medical University, Harbin, China.
authors.forename: X F
authors.initials: XF
authors.lastname: Guo
authors.affiliation: Department of Oncology, The Second Affiliated Hospital, Harbin Medical University, Harbin, China.
authors.forename: W J
authors.initials: WJ
authors.lastname: Sun
authors.affiliation: Department of Breast Surgery, The Second Affiliated Hospital, Harbin Medical University, Harbin, China.
authors.forename: Z Q
authors.initials: ZQ
authors.lastname: Yan
authors.affiliation: Department of Oncology, The Second Affiliated Hospital, Harbin Medical University, Harbin, China.
authors.forename: W X
authors.initials: WX
authors.lastname: Wang
authors.affiliation: Department of Oncology, The Second Affiliated Hospital, Harbin Medical University, Harbin, China.
authors.forename: Y Q
authors.initials: YQ
authors.lastname: Xu
authors.affiliation: Department of Oncology, The Second Affiliated Hospital, Harbin Medical University, Harbin, China.
authors.forename: D
authors.initials: D
authors.lastname: Lu
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000187:drug effects; D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000627:therapeutic use*; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000235:genetics; D002277:Carcinoma / Q000188:drug therapy / Q000235:genetics; D004195:Disease Models, Animal; D004317:Doxorubicin / Q000627:therapeutic use*; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D005260:Female; D020868:Gene Silencing; D019450:Genes, MDR; D005822:Genetic Vectors / Q000235:genetics; D006131:Growth Inhibitors / Q000235:genetics; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics* / Q000378:metabolism; D016086:Lentivirus / Q000235:genetics; D061986:MCF-7 Cells; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D016159:Tumor Suppressor Protein p53 / Q000187:drug effects
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000903:Antibiotics, Antineoplastic; C489114:CIAPIN1 protein, human; D006131:Growth Inhibitors; D047908:Intracellular Signaling Peptides and Proteins; D034741:RNA, Small Interfering; D016159:Tumor Suppressor Protein p53; D004317:Doxorubicin