title: Structural investigation of rhamnose-rich polysaccharides from Streptococcus dysgalactiae bovine mastitis isolate.
abstract: Different rhamnose-rich polysaccharides (RRP) were identified in the cell envelope of the Gram-positive bovine mastitis isolate Streptococcus dysgalactiae 2023. Structural investigations of the 1D and 2D nuclear magnetic resonance experiments as well as chemical analyses identified as main components l-Rha and d-GalNAc. Two main RRP were characterized, namely 1 being composed of the repeating unit {→3)-α-l-Rhap-(1→2)-[α-d-GalpNAc-(1→3)-β-d-GalpNAc-(1→3)-]α-l-Rhap-(1} and 2 possessing the repeat [→2)-α-l-Rhap-(1→3)-α-l-Rhap-(1→].
authors.affiliation: Division of Structural Biochemistry, Priority Area of Asthma and Allergy, Research Center Borstel, Leibniz-Center for Medicine and Biosciences, Airway Research Center North (ARCN), Member of the German Center for Lung Research (DZL), Parkallee 4a/c, D-23845 Borstel, Germany.
authors.forename: Olga
authors.initials: O
authors.lastname: Neiwert
authors.affiliation: Division of Structural Biochemistry, Priority Area of Asthma and Allergy, Research Center Borstel, Leibniz-Center for Medicine and Biosciences, Airway Research Center North (ARCN), Member of the German Center for Lung Research (DZL), Parkallee 4a/c, D-23845 Borstel, Germany.
authors.forename: Otto
authors.initials: O
authors.lastname: Holst
authors.affiliation: Division of Structural Biochemistry, Priority Area of Asthma and Allergy, Research Center Borstel, Leibniz-Center for Medicine and Biosciences, Airway Research Center North (ARCN), Member of the German Center for Lung Research (DZL), Parkallee 4a/c, D-23845 Borstel, Germany. Electronic address: kduda@fz-borstel.de.
authors.forename: Katarzyna A
authors.initials: KA
authors.lastname: Duda
mesh_terms: D000818:Animals; D002240:Carbohydrate Sequence; D002417:Cattle; D008414:Mastitis, Bovine / Q000382:microbiology*; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000737:chemistry*; D012210:Rhamnose / Q000737:chemistry*; D013291:Streptococcus / Q000737:chemistry* / Q000502:physiology*
chemical_list: D011134:Polysaccharides; D012210:Rhamnose
keywords: Mastitis; NMR analysis; Rhamnan; Streptococcus dysgalactiae