title: Molecular cloning, expression, and regulation of estrogen receptors in pigeon oviduct epithelial cells.
abstract: Estrogen regulates reproductive behavior and drives the proliferation and differentiation of several cell types. These physiological functions of estrogen are mediated by estrogen receptors (ERs), and each ER isoform plays a distinct role. To clarify the molecular mechanism of estrogen action and to evaluate the effect of ERs on the secretion of ovalbumin (OVA) in pigeon oviduct epithelial cells (POECs), we determined the complete coding sequences encoding ER alpha (ERα) and ER beta (ERβ) in pigeons. The abundance of pigeon ERα and ERβ mRNA was detected using quantitative polymerase chain reaction. These results revealed that pigeon ERα is highly expressed in the oviduct, while pigeon ERb is highly expressed in the ovary and kidney. We hypothesize that ERα mRNA predominates over that of ERβ in the oviduct. The expression of ERα can be down-regulated by 17β-estradiol, and the knockdown of ERα promoted OVA mRNA expression in cultured POECs, indicating that ERα may play an important role in OVA secretion.
authors.affiliation: Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang, China.
authors.forename: H
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang, China.
authors.forename: F
authors.initials: F
authors.lastname: Chen
authors.affiliation: Ping Yang Science and Technology Bureau, Wenzhou, Zhejiang, China.
authors.forename: G L
authors.initials: GL
authors.lastname: Li
authors.affiliation: Ping Yang Science and Technology Bureau, Wenzhou, Zhejiang, China.
authors.forename: Y Y
authors.initials: YY
authors.lastname: Ding
authors.affiliation: Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang, China.
authors.forename: Z R
authors.initials: ZR
authors.lastname: Tao
authors.affiliation: Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang, China.
authors.forename: J J
authors.initials: JJ
authors.lastname: Li
authors.affiliation: PingYang XingLiang Pigeon Farming Co. Ltd., Wenzhou, Zhejiang, China.
authors.forename: S L
authors.initials: SL
authors.lastname: Zhong
authors.affiliation: Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang, China lulizhibox@163.com.
authors.forename: L Z
authors.initials: LZ
authors.lastname: Lu
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D010856:Columbidae / Q000235:genetics; D004847:Epithelial Cells / Q000378:metabolism*; D004958:Estradiol / Q000378:metabolism; D047628:Estrogen Receptor alpha / Q000096:biosynthesis / Q000235:genetics*; D047629:Estrogen Receptor beta / Q000096:biosynthesis / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D010057:Oviducts / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis
chemical_list: D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D012333:RNA, Messenger; D004958:Estradiol