title: Lysophosphatidic acid receptor-5 negatively regulates cell motile and invasive activities of human sarcoma cell lines.
abstract: LPA signaling via LPA receptors [LPA receptor-1 (LPA1)-LPA6] mediates the several cellular responses in cancer cells, including cell motility and invasion. In the present study, to investigate a role of LPA5 in the cell motile and invasive activities of sarcoma cells, LPAR5 knockdown (HOSL5 and HT1080L5) cells were generated from human osteosarcoma HOS and fibrosarcoma HT1080 cells, respectively. In cell motility assays with cell culture inserts, HOSL5 and HT1080L5 cells indicated the high cell motile activities, compared with control cells. The cell invasive activities of HOSL5 and HT1080L5 cells were significantly higher than those of control cells. Moreover, the activities of matrix metalloproteinase (MMP)-2 and MMP-9 were measured by gelatin zymography. MMP-2 was significantly activated in HOSL5 cells, but not MMP-9. The elevated activities of MMP-2 and MMP-9 were found in HT1080L5 cells, in comparison with control cells. These results suggest that LPA signaling via LPA5 negatively regulates the cell motile and invasive activities of human sarcoma cells.
authors.affiliation: Division of Cancer Biology and Bioinformatics, Department of Life Science, Faculty of Science and Engineering, Kinki University, 3-4-1, Kowakae, Higashiōsaka, Osaka, 577-8502, Japan.
authors.forename: Yan
authors.initials: Y
authors.lastname: Dong
authors.forename: Miku
authors.initials: M
authors.lastname: Hirane
authors.forename: Mutsumi
authors.initials: M
authors.lastname: Araki
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Fukushima
authors.forename: Kanya
authors.initials: K
authors.lastname: Honoki
authors.forename: Toshifumi
authors.initials: T
authors.lastname: Tsujiuchi
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D006801:Humans; D008246:Lysophospholipids / Q000235:genetics / Q000378:metabolism*; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis; D009361:Neoplasm Invasiveness / Q000235:genetics; D049368:Receptors, Lysophosphatidic Acid / Q000096:biosynthesis / Q000235:genetics*; D012509:Sarcoma / Q000235:genetics* / Q000473:pathology; D015398:Signal Transduction
chemical_list: C512992:LPAR5 protein, human; D008246:Lysophospholipids; D049368:Receptors, Lysophosphatidic Acid; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; C032881:lysophosphatidic acid