title: Explaining the correlations among properties of mammalian promoters.
abstract: Proximal promoters are fundamental genomic elements for gene expression. They vary in terms of GC percentage, CpG abundance, presence of TATA signal, evolutionary conservation, chromosomal spread of transcription start sites and breadth of expression across cell types. These properties are correlated, and it has been suggested that there are two classes of promoters: one class with high CpG, widely spread transcription start sites and broad expression, and another with TATA signals, narrow spread and restricted expression. However, it has been unclear why these properties are correlated in this way. We reexamined these features using the deep FANTOM5 CAGE data from hundreds of cell types. First, we point out subtle but important biases in previous definitions of promoters and of expression breadth. Second, we show that most promoters are rather nonspecifically expressed across many cell types. Third, promoters' expression breadth is independent of maximum expression level, and therefore correlates with average expression level. Fourth, the data show a more complex picture than two classes, with a network of direct and indirect correlations among promoter properties. By tentatively distinguishing the direct from the indirect correlations, we reveal simple explanations for them.
authors.affiliation: Computational Biology Research Center, AIST, 2-4-7 Aomi, Koto-ku, Tokyo 135-0064, Japan.
authors.forename: Martin C
authors.initials: MC
authors.lastname: Frith
mesh_terms: D000818:Animals; D018899:CpG Islands; D003627:Data Interpretation, Statistical; D006801:Humans; D051379:Mice; D011401:Promoter Regions, Genetic; D016385:TATA Box; D024363:Transcription Initiation Site; D014158:Transcription, Genetic