title: Association of functional polymorphisms in FAS and FAS Ligand genes promoter with pre-eclampsia.
abstract: OBJECTIVE
  Pre-eclampsia (PE) is a complex disorder of pregnancy with unknown etiology. FAS-mediated apoptosis is assumed to prevent the development of PE; therefore FAS and FAS Ligand may be represented as candidate genes involved in PE pathogenesis. In the present study, we evaluated the relation between FAS Ligand A-670G (rs1800682) and FAS Ligand C-844T (rs763110) gene polymorphisms with PE in southeast Iran.
  
  
  METHODS
  One hundred and twenty-seven unrelated women with PE and 139 healthy control subjects were genotyped for the FAS A-670G and FAS Ligand C-844T polymorphisms by polymerase chain reaction restriction fragment length polymorphism method.
  
  
  RESULTS
  The AA, AG and GG genotype frequency of the FAS A-670G polymorphism were 21.3%, 53.5% and 25.2% in pre-eclamptic women and 46.0%, 41.5% and 11.5% in controls and were statistically different (P = 0.0001). The risk of PE was 2.7- and 4.7-fold higher in pregnant women with AG and GG genotypes respectively. Although the frequency TT genotype and T allele of FAS Ligand C-844T gene polymorphism was higher in the PE group, the differences were not significant.
  
  
  CONCLUSIONS
  FAS A-670G polymorphism is associated with a higher risk for PE. There was no association between FAS Ligand C-844T polymorphism and PE.
authors.affiliation: Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan, Iran; Department of Clinical Biochemistry, Zahedan University of Medical Sciences, Zahedan, Iran.
authors.forename: Saeedeh
authors.initials: S
authors.lastname: Salimi
authors.forename: Bita
authors.initials: B
authors.lastname: Moudi
authors.forename: Farzaneh
authors.initials: F
authors.lastname: Farajian Mashhadi
authors.forename: Heidar
authors.initials: H
authors.lastname: Tavilani
authors.forename: Mohammad
authors.initials: M
authors.lastname: Hashemi
authors.forename: Hamid
authors.initials: H
authors.lastname: Zand
authors.forename: Minoo
authors.initials: M
authors.lastname: Yaghmaei
mesh_terms: D000328:Adult; D053222:Fas Ligand Protein / Q000235:genetics*; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D020641:Polymorphism, Single Nucleotide; D011225:Pre-Eclampsia / Q000235:genetics*; D011247:Pregnancy; D011401:Promoter Regions, Genetic; D019014:fas Receptor / Q000235:genetics*
chemical_list: C491901:FAS protein, human; C505661:FASLG protein, human; D053222:Fas Ligand Protein; D019014:fas Receptor
keywords: FAS; FAS Ligand; polymorphism; pre-eclampsia