title: DNA comparison between operative and biopsy specimens to investigate stage pT0 after radical prostatectomy.
abstract: OBJECTIVE
  The aim was to eliminate, by DNA comparison, any identity mismatch between operative and biopsy specimens and to analyse the determinants of all pT0 prostate cancers occurred in a single institution.
  
  
  METHODS
  All prostate pT0 cases in a single institution over 20 years were investigated. None of the patients had been diagnosed after a transurethral resection of the prostate nor had they received neoadjuvant hormonal treatment. The biopsies performed in other centres had been referred for a centralized pathologic re-analysis. DNA analysis was performed in samples from operative and biopsy specimens, and pairs of tissues were blindly constituted. Correct matching was verified in each pair and compared to the original database in order to comment on the occurrence of identity mismatches in the series.
  
  
  RESULTS
  Nineteen patients (0.77 %) had been diagnosed as having pT0 prostate cancer among the 2,462 RP procedures performed over 19 years. The biopsy re-analysis invalidated the initial diagnosis of prostate cancer in one biopsy set performed elsewhere. Among 12 entirely processed cases, the biochemistry procedure evaluated as "very unlikely" the occurrence of an error in tissue identification in the biopsy setting, during the surgical procedure or the pathological analysis. No identification error of tissue samples was established in this first verified pT0 series.
  
  
  CONCLUSIONS
  Although it must be suspected, specimen identification error was not a cause for pT0 prostate cancer. Only after a full pathological and DNA verification, the pT0 stage remains a sole entity, unexplained in most cases.
authors.affiliation: Department of Urology, Paris Sud University Hospital of Bicetre, 94270, Le Kremlin Bicetre, France, thomas.bessede@gmail.com.
authors.forename: Thomas
authors.initials: T
authors.lastname: Bessede
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Girodon
authors.forename: Yves
authors.initials: Y
authors.lastname: Allory
authors.forename: Annick
authors.initials: A
authors.lastname: Le Floch
authors.forename: Karen
authors.initials: K
authors.lastname: Leroy
authors.forename: Laurent
authors.initials: L
authors.lastname: Salomon
mesh_terms: D000368:Aged; D001706:Biopsy; D004273:DNA, Neoplasm / Q000032:analysis / Q000235:genetics*; D004276:DNA, Satellite / Q000032:analysis / Q000235:genetics; D006801:Humans; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011467:Prostate / Q000473:pathology; D011468:Prostatectomy; D011471:Prostatic Neoplasms / Q000175:diagnosis / Q000473:pathology* / Q000601:surgery*; D012189:Retrospective Studies
chemical_list: D004273:DNA, Neoplasm; D004276:DNA, Satellite