title: Evolutionary dynamics of endogenous Jaagsiekte sheep retroviruses proliferation in the domestic sheep, mouflon and Pyrenean chamois.
abstract: The oncogenic exogenous Jaagsiekte sheep retrovirus (JSRV), responsible for ovine pulmonary adenocarcinoma, has several endogenous counterparts termed enJSRVs. Although many of these elements have been inactivated over time by the accumulation of deleterious mutations or internal recombination leading to solo long terminal repeat (LTR) formation, several members of enJSRVs have been identified as nearly intact and probably represent recent integration events. To determine the level of enJSRV polymorphism in the sheep population and related species, we have undertaken a study by characterizing enJSRVs copies and independent integration sites in six domestic sheep and two wild species of the sheep lineage. enJSRVs copies were detected by amplifying the env-LTR region by PCR, and for the detection of the insertion sites, we used two approaches: (1) an in silico approach based on the recently published Sheep Reference Genome Assembly (OARv3.0) and (2) an experimental approach based on PCR suppression and inverse PCR techniques. In total, 103 enJSRV sequences were generated across 10 individuals and enJSRV integrations were found on 11 of the 28 sheep chromosomes. These findings suggest that there are still uncharacterized enJSRVs, and that some of the integration sites are variable among the different species, breeds of the same species, subspecies and geographic locations.
authors.affiliation: Genetics, Physical Anthropology and Animal Physiology Department, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), Bilbao, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Sistiaga-Poveda
authors.affiliation: Genetics, Physical Anthropology and Animal Physiology Department, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), Bilbao, Spain.
authors.forename: B M
authors.initials: BM
authors.lastname: Jugo
mesh_terms: D000818:Animals; D019295:Computational Biology; D020077:Endogenous Retroviruses; D019143:Evolution, Molecular; D023061:Gene Order; D014644:Genetic Variation; D016679:Genome, Viral; D022141:Jaagsiekte sheep retrovirus / Q000145:classification / Q000502:physiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011110:Polymorphism, Genetic; D048608:Rupicapra / Q000821:virology*; D012756:Sheep; D034561:Sheep, Domestic / Q000821:virology*; D020079:Terminal Repeat Sequences; D016662:Virus Integration; D014779:Virus Replication