title: microRNA-200a inhibits cell proliferation by targeting mitochondrial transcription factor A in breast cancer.
abstract: microRNAs (miRNAs) are endogenous 19-25 nucleotide noncoding single-stranded RNAs that regulate gene expression by blocking the translation or decreasing the stability of mRNAs. In this study, we showed that miR-200a expression levels were decreased while mitochondrial transcription factor A (TFAM) mRNA expression levels were increased in breast cancer (BC) tissues and cell lines, and identified TFAM as a novel direct target of miR-200a. Overexpression of miR-200a suppressed TFAM protein expression, mtDNA copy number, and attenuated cell proliferation. Forced expression of TFAM can partly rescue the inhibitory effect of miR-200a in the cells. Taken together, these findings will shed light on the role and mechanism of miR-200a in regulating BC cells growth and mtDNA copy number via miR-200a/TFAM axis, and miR-200a may serve as a potential therapeutic target in BC in the future.
authors.affiliation: 1 Department of General Surgery, The Second Xiangya Hospital of Central South University , Changsha, China .
authors.forename: Jia
authors.initials: J
authors.lastname: Yao
authors.forename: Enxiang
authors.initials: E
authors.lastname: Zhou
authors.forename: Yichun
authors.initials: Y
authors.lastname: Wang
authors.forename: Feng
authors.initials: F
authors.lastname: Xu
authors.forename: Danhua
authors.initials: D
authors.lastname: Zhang
authors.forename: Dewu
authors.initials: D
authors.lastname: Zhong
mesh_terms: D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D049109:Cell Proliferation; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D061986:MCF-7 Cells; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; C533896:MIRN200 microRNA, human; D035683:MicroRNAs; D024101:Mitochondrial Proteins; C499631:TFAM protein, human; D014157:Transcription Factors