title: Effect of ammonium nitrogen concentration on the ammonia-oxidizing bacteria community in a membrane bioreactor for the treatment of anaerobically digested swine wastewater.
abstract: A membrane bioreactor (MBR) was developed for the treatment of anaerobically digested swine wastewater and to investigate the effect of ammonium nitrogen concentration on biological nitrogen removal and ammonia-oxidizing bacteria (AOB) community structures. The MBR achieved a high NH4(+)-N removal efficiency of 0.08 kgNMLSS(-1)d(-1) and removed 95% of the influent NH4(+)-N. The TN removal rate was highest of 82.62% at COD/TN and BOD5/TN ratios of 8.76 ± 0.30 and 3.02 ± 0.09, respectively. With the decrease in ammonium nitrogen concentrations, the diversity of the AOB community declined and showed a simple pattern of DGGE. However, the AOB population size remained high, with abundance of 10(7)-10(9) copies mL(-1). With the decrease of ammonium nitrogen concentrations, Nitrosomonas eutropha gradually disappeared, whereas Nitrosomonas sp. OZK11 showed constant adaptability to survive during each treatment stage. The selective effect of ammonium concentration on AOB species could be due to the affinity for NH4(+)-N. In this study, the changes of ammonium nitrogen concentrations in digested swine wastewater were found to have selective effects on the composition of AOB community, and biological nitrogen removal was improved by optimising the influencing parameters.
authors.affiliation: Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100081, China; The Key Laboratory of Energy Conservation and Waste Treatment of Agricultural Structures, Ministry of Agriculture, Beijing 100081, China.
authors.forename: Qianwen
authors.initials: Q
authors.lastname: Sui
authors.affiliation: Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100081, China; The Key Laboratory of Energy Conservation and Waste Treatment of Agricultural Structures, Ministry of Agriculture, Beijing 100081, China.
authors.forename: Chong
authors.initials: C
authors.lastname: Liu
authors.affiliation: Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100081, China; The Key Laboratory of Energy Conservation and Waste Treatment of Agricultural Structures, Ministry of Agriculture, Beijing 100081, China. Electronic address: donghm@ieda.org.cn.
authors.forename: Hongmin
authors.initials: H
authors.lastname: Dong
authors.affiliation: Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100081, China; The Key Laboratory of Energy Conservation and Waste Treatment of Agricultural Structures, Ministry of Agriculture, Beijing 100081, China.
authors.forename: Zhiping
authors.initials: Z
authors.lastname: Zhu
mesh_terms: D064751:Ammonium Compounds / Q000737:chemistry / Q000378:metabolism*; D000693:Anaerobiosis; D000822:Animal Husbandry; D000818:Animals; D001673:Biodegradation, Environmental; D019149:Bioreactors; D059013:Microbial Consortia; D009606:Nitrosomonas / Q000145:classification / Q000378:metabolism*; D013552:Swine; D014865:Waste Disposal, Fluid; D062065:Wastewater / Q000382:microbiology*
chemical_list: D064751:Ammonium Compounds; D062065:Wastewater
keywords: Ammonia-oxidizing bacteria; Anaerobically digested wastewater; Biological nitrogen removal; Membrane bioreactor; Nitrification