title: Herpes simplex virus growth, preparation, and assay.
abstract: In order to study the biology of herpes simplex virus or to use it as a vector in gene therapy, it is necessary to grow the virus and to prepare virus stocks. Many different protocols are available from different research groups working with herpes simplex virus type 1 or 2 (HSV-1 or HSV-2). This chapter describes the procedures used in our laboratory.
authors.affiliation: Section of Applied Microbiology and Pathology, Department of Life Sciences and Biotechnology (SVeB), University of Ferrara, Via Luigi Borsari 46, Ferrara, 44121, Italy, mcy@unife.it.
authors.forename: Peggy
authors.initials: P
authors.lastname: Marconi
authors.forename: Roberto
authors.initials: R
authors.lastname: Manservigi
mesh_terms: D015316:Genetic Therapy; D005822:Genetic Vectors; D018259:Herpesvirus 1, Human / Q000235:genetics* / Q000254:growth & development / Q000472:pathogenicity; D018258:Herpesvirus 2, Human / Q000235:genetics* / Q000254:growth & development / Q000472:pathogenicity; D006801:Humans; D008967:Molecular Biology / Q000379:methods*