title: Gene expression and morphometric parameters of human bone biopsies after maxillary sinus floor elevation with autologous bone combined with Bio-Oss® or BoneCeramic®.
abstract: OBJECTIVE
  Although the clinical success of Bio-Oss(®) and BoneCeramic(®) has been corroborated by histologic and histomorphometric findings, the biological events that occur during healing after maxillary sinus floor elevation (MSFE) are unknown. Here, we evaluated biopsies of grafted bone with a mixture of autologous bone and Bio-Oss(®) or BoneCeramic(®) after two different healing time periods to understand the molecular process underlying bone formation after MSFE.
  
  
  METHODS
  Seven patients, following a bilateral split-mouth design model and needing a MSFE to allow implant placement, were recruited for this study. Right or left sinuses were grafted with autologous maxillary bone combined either with Bio-Oss(®) or BoneCeramic(®) , respectively. Twenty biopsies were taken at the time of implant insertion after 4-5 months or 6-8 months of MSFE, and analyzed by micro-computed tomography (microCT) and gene-expression analysis.
  
  
  RESULTS
  MicroCT analysis revealed no differences in the morphometric parameters or BMD either after 4-5 months or 6-8 months of MSFE between Bio-Oss(®) and BoneCeramic(®) . At molecular level, a higher expression of bone forming gene Runx2 was observed after 4-5 months of MSFE in the Bio-Oss(®) compared with the BoneCeramic(®) group.
  
  
  CONCLUSIONS
  Our results indicate that differences found at the molecular level between Bio-Oss(®) and BoneCeramic(®) are not translated to important differences in the 3D microstructure and BMD of the grafted bone.
authors.affiliation: Bone regeneration and Oral and Maxillofacial Surgery Unit (GBCOM), Palma de Mallorca, Spain.
authors.forename: Jorge
authors.initials: J
authors.lastname: Caubet
authors.forename: Joana M
authors.initials: JM
authors.lastname: Ramis
authors.forename: Mikel
authors.initials: M
authors.lastname: Ramos-Murguialday
authors.forename: Miguel Á
authors.initials: MÁ
authors.lastname: Morey
authors.forename: Marta
authors.initials: M
authors.lastname: Monjo
mesh_terms: D000328:Adult; D001706:Biopsy; D001861:Bone Regeneration / Q000502:physiology*; D018786:Bone Substitutes / Q000627:therapeutic use*; D016025:Bone Transplantation / Q000379:methods*; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics / Q000378:metabolism; D005260:Female; D006801:Humans; D006882:Hydroxyapatites / Q000627:therapeutic use*; D008297:Male; D008437:Maxilla / Q000601:surgery; D008443:Maxillary Sinus / Q000601:surgery; D008875:Middle Aged; D008903:Minerals / Q000627:therapeutic use*; D010012:Osteogenesis / Q000502:physiology; D059546:Sinus Floor Augmentation / Q000379:methods*
chemical_list: C077540:Bio-Oss; D018786:Bone Substitutes; C558777:BoneCeramic; D050659:Core Binding Factor Alpha 1 Subunit; D006882:Hydroxyapatites; D008903:Minerals; C493709:RUNX2 protein, human
keywords: bone graft; clinical study; dental implant; gene expression; maxillary sinus floor elevation (MSFE); micro-computed tomography (microCT)