title: Carbon nanotube-based substrates for modulation of human pluripotent stem cell fate.
abstract: We investigated the biological response of human pluripotent stem cells (hPSCs) cultured on a carbon nanotube (CNT) array-based substrate with the long term goal to direct hPSC germ layer specification for a wide variety of tissue engineering applications. CNT arrays were fabricated using a chemical vapor deposition system allowing for control over surface roughness and mechanical stiffness. Our results demonstrated that hPSCs readily attach to hydrophilized and extracellular matrix coated CNT arrays. hPSCs cultured as colonies in conditions supporting self-renewal demonstrated the morphology and marker expression of undifferentiated hPSCs. Conditions inducing spontaneous differentiation lead to hPSC commitment to all three embryonic germ layers as assessed by immunostaining and RT-PCR analysis. Strikingly, the physical characteristics of CNT arrays favored mesodermal specification of hPSCs. This is contradictory to the behavior of hPSCs on traditional tissue culture plastic which promotes the development of ectoderm. Altogether, these results demonstrate the potential of CNT arrays to be used in the generation of new platforms that allow for precise control of hPSC differentiation by tuning the characteristics of their physical microenvironment.
authors.affiliation: Department of Chemical Engineering, University of South Carolina, SC 29208, USA.
authors.forename: Marina V
authors.initials: MV
authors.lastname: Pryzhkova
authors.affiliation: Graduate Aeronautics Laboratories, California Institute of Technology, CA 91125, USA.
authors.forename: Indrat
authors.initials: I
authors.lastname: Aria
authors.affiliation: Department of Biomedical Engineering, University of South Carolina, SC 29208, USA.
authors.forename: Qingsu
authors.initials: Q
authors.lastname: Cheng
authors.affiliation: Department of Chemical Engineering, University of South Carolina, SC 29208, USA.
authors.forename: Greg M
authors.initials: GM
authors.lastname: Harris
authors.affiliation: Department of Chemistry and Biochemistry, University of South Carolina, SC 29208, USA.
authors.forename: Xingjie
authors.initials: X
authors.lastname: Zan
authors.affiliation: Graduate Aeronautics Laboratories, California Institute of Technology, CA 91125, USA.
authors.forename: Morteza
authors.initials: M
authors.lastname: Gharib
authors.affiliation: Department of Chemical Engineering, University of South Carolina, SC 29208, USA; Department of Biomedical Engineering, University of South Carolina, SC 29208, USA; Department of Orthopaedic Surgery, University of South Carolina, SC 29208, USA. Electronic address: jabbarza@cec.sc.edu.
authors.forename: Ehsan
authors.initials: E
authors.lastname: Jabbarzadeh
mesh_terms: D002448:Cell Adhesion / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line; D003599:Cytoskeleton / Q000378:metabolism; D006801:Humans; D037742:Nanotubes, Carbon / Q000737:chemistry*; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism
chemical_list: D037742:Nanotubes, Carbon
keywords: Cell adhesion; Cytoskeleton; Differentiation; Human pluripotent stem cells; Multi-walled carbon nanotubes; Surface roughness