title: Characterization and antioxidative activities of polysaccharide in Chinese angelica and its processed products.
abstract: Five polysaccharides from unprocessed Chinese angelica (UCAP), parched one with alcohol (ACAP), soil (SCAP), sesame oil (OCAP) and parched into charred (CCAP) were extracted and purified. Their structures were identified by Fourier transform-infrared spectroscopy (FT-IR), compositions were analyzed by gas chromatography-mass spectrometry (GC-MS) and antioxidative activities were compared by determining MDA contents and SOD activities of liver tissue in mice damaged with CCl4 after gavage. The results showed that the FT-IR spectra of CCAP and OCAP displayed lower transmittance at around 1050cm(-1) in comparison with that of UCAP. Five polysaccharides were all composed of rhamnose, arabinose, mannose, glucose and galactose. In CCAP, ACAP, OCAP and SCAP, the proportions of arabinose were significantly increased in comparison with that of UCAP. The SOD activities in CCAP and SCAP groups were significantly enhanced, and MDA contents in CCAP, OCAP and SCAP groups were significantly decreased as compared with UCAP group. This indicated that processing could change the structure, composition and enhance antioxidative activity of polysaccharide in Chinese angelica, and CCAP possessed the strongest antioxidative activity.
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, PR China.
authors.forename: Peng
authors.initials: P
authors.lastname: Ji
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, PR China. Electronic address: weiym@gsau.edu.cn.
authors.forename: Yanming
authors.initials: Y
authors.lastname: Wei
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, PR China.
authors.forename: Wenxin
authors.initials: W
authors.lastname: Xue
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, PR China.
authors.forename: Yongli
authors.initials: Y
authors.lastname: Hua
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, PR China.
authors.forename: Man
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, PR China.
authors.forename: Hongguo
authors.initials: H
authors.lastname: Sun
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, PR China.
authors.forename: Zhixue
authors.initials: Z
authors.lastname: Song
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, PR China.
authors.forename: Ling
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, PR China.
authors.forename: Jinxia
authors.initials: J
authors.lastname: Li
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, PR China.
authors.forename: Haifu
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, PR China.
authors.forename: Wenquan
authors.initials: W
authors.lastname: Zhang
mesh_terms: D029971:Angelica sinensis / Q000737:chemistry*; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage* / Q000737:chemistry / Q000302:isolation & purification; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry*; D005690:Galactose / Q000737:chemistry; D005947:Glucose / Q000737:chemistry / Q000302:isolation & purification; D008099:Liver / Q000187:drug effects; D008358:Mannose / Q000737:chemistry; D051379:Mice; D011134:Polysaccharides / Q000008:administration & dosage / Q000737:chemistry / Q000302:isolation & purification*; D012210:Rhamnose / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D011134:Polysaccharides; C044613:angelicae sinensis extract; D005947:Glucose; D008358:Mannose; D012210:Rhamnose; D005690:Galactose
keywords: Antioxidative activities; Characterization; Polysaccharides in Chinese angelica and its different processed products