title: A multivariate calibration procedure for UV/VIS spectrometric monitoring of BHK-21 cell metabolism and growth.
abstract: Monitoring mammalian cell culture with UV–vis spectroscopy has not been widely explored. The aim of this work was to calibrate Partial Least Squares (PLS) models from off-line UV–vis spectral data in order to predict some nutrients and metabolites, as well as viable cell concentrations for mammalian cell bioprocess using phenol red in culture medium. The BHK-21 cell line was used as a mammalian cell model. Spectra of samples taken from batches performed at different dissolved oxygen concentrations (10, 30, 50, and 70% air saturation), in two bioreactor configurations and with two strategies to control pH were used to calibrate and validate PLS models. Glutamine, glutamate, glucose, and lactate concentrations were suitably predicted by means of this strategy. Especially for glutamine and glucose concentrations, the prediction error averages were lower than 0.5060.10 mM and 2.2160.16 mM, respectively. These values are comparable with those previously reported using near infrared and Raman spectroscopy in conjunction with PLS. However, viable cell concentration models need to be improved. The present work allows for UV–vis at-line sensor development, decrease cost related to nutrients and metabolite quantifications and establishment of fed-batch feeding schemes.
authors.forename: Jaci
authors.initials: J
authors.lastname: Leme
authors.forename: Eutimio Gustavo
authors.initials: EG
authors.lastname: Fernández Núñez
authors.forename: Letícia
authors.initials: L
authors.lastname: de Almeida Parizotto
authors.forename: Wagner Antonio
authors.initials: WA
authors.lastname: Chagas
authors.forename: Erica
authors.initials: E
authors.lastname: Salla dos Santos
authors.forename: Aline
authors.initials: A
authors.lastname: Tojeira Prestia Caricati
authors.forename: Alexandre
authors.initials: A
authors.lastname: Gonçalves de Rezende
authors.forename: Bruno
authors.initials: B
authors.lastname: Labate Vale da Costa
authors.forename: Daniela Cristina
authors.initials: DC
authors.lastname: Ventini Monteiro
authors.forename: Vera Lucia
authors.initials: VL
authors.lastname: Lopes Boldorini
authors.forename: Soraia Attie
authors.initials: SA
authors.lastname: Calil Jorge
authors.forename: Renato
authors.initials: R
authors.lastname: Mancini Astray
authors.forename: Carlos Augusto
authors.initials: CA
authors.lastname: Pereira
authors.forename: Celso
authors.initials: C
authors.lastname: Pereira Caricati
authors.forename: Tonso
authors.initials: T
authors.lastname: Tonso
mesh_terms: D000818:Animals; D019149:Bioreactors; D002138:Calibration; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D049109:Cell Proliferation; D003470:Culture Media / Q000737:chemistry / Q000378:metabolism; D016018:Least-Squares Analysis; D015999:Multivariate Analysis; D010100:Oxygen / Q000032:analysis / Q000378:metabolism; D017693:Sodium Bicarbonate / Q000032:analysis / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
chemical_list: D003470:Culture Media; D017693:Sodium Bicarbonate; D010100:Oxygen