title: Synthesis and biological evaluation of arabinose 5-phosphate mimics modified at position five.
abstract: A set of new metabolically stable arabinose 5-phosphate analogues possessing phosphate mimetic groups at position 5 was synthesised. Their ability to interact with arabinose 5-phosphate isomerase from Pseudomonas aeruginosa was evaluated by STD-NMR studies. The synthesised compounds were also characterised for their activity in vivo on P. aeruginosa and Escherichia coli strains. Unfortunately, none of the synthesised compounds was able neither to bind API nor to inhibit bacterial growth.
authors.affiliation: Department of Biotechnology and Biosciences, Università degli Studi Milano-Bicocca, Piazza della Scienza 2, Milan, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Cipolla
authors.affiliation: Department of Biotechnology and Biosciences, Università degli Studi Milano-Bicocca, Piazza della Scienza 2, Milan, Italy.
authors.forename: Cristina
authors.initials: C
authors.lastname: Airoldi
authors.affiliation: Department of Biotechnology and Biosciences, Università degli Studi Milano-Bicocca, Piazza della Scienza 2, Milan, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Sperandeo
authors.affiliation: Department of Biotechnology and Biosciences, Università degli Studi Milano-Bicocca, Piazza della Scienza 2, Milan, Italy.
authors.forename: Serena
authors.initials: S
authors.lastname: Gianera
authors.affiliation: Department of Biotechnology and Biosciences, Università degli Studi Milano-Bicocca, Piazza della Scienza 2, Milan, Italy.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Polissi
authors.affiliation: Department of Biotechnology and Biosciences, Università degli Studi Milano-Bicocca, Piazza della Scienza 2, Milan, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Nicotra
authors.affiliation: Department of Biotechnology and Biosciences, Università degli Studi Milano-Bicocca, Piazza della Scienza 2, Milan, Italy. Electronic address: l.gabrielli2@campus.unimib.it.
authors.forename: Luca
authors.initials: L
authors.lastname: Gabrielli
mesh_terms: D019747:Aldose-Ketose Isomerases / Q000378:metabolism; D040761:Biomimetic Materials / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D060326:Chemistry Techniques, Synthetic; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D009154:Mutation; D010428:Pentosephosphates / Q000737:chemistry*; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development
chemical_list: D010428:Pentosephosphates; C028055:arabinose 5-phosphate; D019747:Aldose-Ketose Isomerases; C041486:arabinose-5-phosphate isomerase
keywords: Arabinose 5-phosphate; NMR interaction studies; Phosphate analogues; Pseudomonas aeruginosa; Sugar isomerase