title: Protein filter binding.
abstract: This protocol describes a method to monitor the binding of nucleic acid to protein, allowing the determination of the apparent affinity of a nucleic acid-protein interaction.
authors.affiliation: Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
authors.forename: Sarah
authors.initials: S
authors.lastname: Kolitz
authors.affiliation: Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA. Electronic address: skolitz@mit.edu.
authors.forename: Jon R
authors.initials: JR
authors.lastname: Lorsch
mesh_terms: D003101:Collodion / Q000737:chemistry; D005374:Filtration / Q000295:instrumentation / Q000379:methods*; D009696:Nucleic Acids / Q000032:analysis / Q000378:metabolism*; D011506:Proteins / Q000032:analysis / Q000378:metabolism*
chemical_list: D009696:Nucleic Acids; D011506:Proteins; D003101:Collodion
keywords: Binding; Nucleic acid–protein interaction; Nytran™ SuPerCharge (SPC) membranes; Protein filter binding; Whatman GF/C filters