title: Production, purification, and antibiofilm activity of a novel exopolysaccharide from Arthrobacter sp. B4.
abstract: A novel exopolysaccharide (EPS), namely, B4-EPS, is produced by Arthrobacter sp. B4. Response surface methodology (RSM) was employed to optimize the fermentation medium for increasing B4-EPS production. Based on Plackett-Burman design (PBD), glucose, yeast extract, and KH2PO4 were selected as significant variables, which were further optimized by a central composite design (CCD). According to response surface and canonical analysis, the optimal medium was composed of 16.94 g/L glucose, 2.33 g/L yeast extract, and 5.32 g/L KH2PO4. Under this condition, the maximum yield of B4-EPS reached about 8.54 g/L after 72 hr of batch fermentation, which was pretty close to the predicted value (8.52 g/L). Furthermore, B4-EPS was refined by column chromatography. The main homogeneous fraction (B4-EPS1) was collected and applied to assay of antibiofilm activity. B4-EPS1 exhibited a dose-dependent inhibitory effect on biofilm formation of Pseudomonas aeruginosa PAO1 without antibacterial activity. About 86.1% of biofilm formation of P. aeruginosa PAO1 was inhibited in the presence of 50 µg/mL B4-EPS1, which was more effective than the peer published data. Moreover, B4-EPS1 could prevent biofilm formation of other strains. These data suggest B4-EPS may represent a promising strategy to combat bacterial biofilms in the future.
authors.affiliation: a School of Bioscience and Biotechnology , University of Jinan , Jinan , China.
authors.forename: Yumei
authors.initials: Y
authors.lastname: Li
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.forename: Dakui
authors.initials: D
authors.lastname: Hao
authors.forename: Dong
authors.initials: D
authors.lastname: Jiang
authors.forename: Yan
authors.initials: Y
authors.lastname: Luo
authors.forename: Yingzi
authors.initials: Y
authors.lastname: Liu
authors.forename: Zhiwen
authors.initials: Z
authors.lastname: Zhao
mesh_terms: D001173:Arthrobacter / Q000737:chemistry / Q000254:growth & development; D018441:Biofilms / Q000187:drug effects*; D003470:Culture Media / Q000737:chemistry; D011135:Polysaccharides, Bacterial / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000502:physiology*
chemical_list: D003470:Culture Media; D011135:Polysaccharides, Bacterial
keywords: Arthrobacter sp; Pseudomonas aeruginosa; antibiofilm; exopolysaccharide; response surface methodology