title: Effect of overexpression of β- and γ-actin isoforms on actin cytoskeleton organization and migration of human colon cancer cells.
abstract: Actins are eukaryotic proteins, which are involved in diverse cellular functions including muscle contraction, cell motility, adhesion and maintenance of cell shape. Cytoplasmic actin isoforms β and γ are ubiquitously expressed and essential for cell functioning. However, their unique contributions are not very well understood. The aim of this study was to determine the effect of β- and γ-actin overexpression on the migration capacity and actin cytoskeleton organization of human colon adenocarcinoma BE cells. In cells overexpressing β- or γ-actin, distinct cytoskeletal actin rearrangements were observed under the laser scanning confocal microscope. Overexpressed actins localized at the submembranous region of the cell body, especially near to the leading edge and on the tips of pseudopodia. The cells transfected with plasmids containing cDNA for β- or γ-actin were characterized by increased migration and invasion capacities. However, the migration velocity was statistically significantly higher only in the case of γ-actin overexpressing cells. In conclusion, the increased level of β- or γ-actin leads to actin cytoskeletal remodeling followed by an increase in migration and invasion capacities of human colon BE cells. These data suggest that expression of both actin isoforms has an impact on cancer cell motility, with the subtle predominance of γ-actin, and may influence invasiveness of human colon cancer.
authors.affiliation: Department of Cell Pathology, Faculty of Biotechnology, University of Wrocław, Joliot-Curie 14a, 50-383, Wrocław, Poland.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Simiczyjew
authors.forename: Antonina Joanna
authors.initials: AJ
authors.lastname: Mazur
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Popow-Woźniak
authors.forename: Maria
authors.initials: M
authors.lastname: Malicka-Błaszkiewicz
authors.forename: Dorota
authors.initials: D
authors.lastname: Nowak
mesh_terms: D008841:Actin Cytoskeleton / Q000235:genetics / Q000378:metabolism*; D000199:Actins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism*; D002465:Cell Movement / Q000235:genetics*; D003110:Colonic Neoplasms / Q000473:pathology*; D006801:Humans; D020033:Protein Isoforms / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: D000199:Actins; D020033:Protein Isoforms