title: Studies on the analgesic activities of Jia-Yuan-Qing pill and its safety evaluation in mice.
abstract: The analgesic activity of Porcellio laevis Latreille, Rhizoma Corydalis, and Radix Cynanchi Paniculati have been reported in recent years. A new formula named Jia-Yuan-Qing pill (JYQP) is therefore created by combining the three herbs at 9:7:7 ratio according to traditional Chinese theories. The present study aims to evaluate the effect of JYQP as a novel painkiller in various models. Acute toxicity test was applied to evaluate the safety of JYQP. Acetic-acid-induced writhing, hot plate test, formalin test, and naloxone-pretreated writhing test were employed to elaborate the analgesic activity of JYQP and its possible mechanism. A bone cancer pain mouse model was performed to further assess the effect of JYQP in relieving cancer pain. Test on naloxone-precipitated withdrawal symptoms was conduct to examine the physical dependence of mice on JYQP. Data revealed that JYQP reduced writhing and stretching induced by acetic acid; however, this effect could not be blocked by naloxone. JYQP specifically suppressed the phase II reaction time in formalin-treated mice; meanwhile, no analgesic effect of JYQP in hot plate test was observed, indicating that JYQP exerts analgesic activity against inflammatory pain rather than neurogenic pain. Furthermore, JYQP could successfully relieve bone cancer pain in mice. No physical dependence could be observed upon long-term administration in mice. Collectively, our present results provide experimental evidence in supporting clinical use of JYQP as an effective and safe agent for pain treatment.
authors.affiliation: College of Life Sciences, Jilin University, No. 2699, Qianjin Avenue, Changchun City, Jilin, 130012, People's Republic of China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Tian
authors.forename: Li-rong
authors.initials: LR
authors.lastname: Teng
authors.forename: Jing-jing
authors.initials: JJ
authors.lastname: Song
authors.forename: Qing-fan
authors.initials: QF
authors.lastname: Meng
authors.forename: Jia-hui
authors.initials: JH
authors.lastname: Lu
authors.forename: Wei-wei
authors.initials: WW
authors.lastname: Zhang
authors.forename: Kang
authors.initials: K
authors.lastname: Wei
authors.forename: Ning
authors.initials: N
authors.lastname: Wang
authors.forename: Di
authors.initials: D
authors.lastname: Wang
authors.forename: Le-sheng
authors.initials: LS
authors.lastname: Teng
mesh_terms: D000700:Analgesics / Q000009:adverse effects / Q000494:pharmacology*; D000818:Animals; D001859:Bone Neoplasms / Q000473:pathology; D031311:Corydalis / Q000378:metabolism*; D031105:Cynanchum / Q000378:metabolism*; D005260:Female; D007249:Inflammation / Q000473:pathology; D033321:Isopoda / Q000378:metabolism*; D008297:Male; D008516:Medicine, Chinese Traditional / Q000009:adverse effects; D051379:Mice; D008810:Mice, Inbred C57BL; D009270:Naloxone / Q000494:pharmacology; D009292:Narcotic Antagonists / Q000494:pharmacology; D010146:Pain / Q000188:drug therapy*; D008517:Phytotherapy; D010936:Plant Extracts / Q000009:adverse effects / Q000494:pharmacology
chemical_list: D000700:Analgesics; D009292:Narcotic Antagonists; D010936:Plant Extracts; D009270:Naloxone