title: Supported liquid membrane as a novel tool for driving the equilibrium of ω-transaminase catalyzed asymmetric synthesis.
abstract: An attractive option to produce chiral amines of industrial importance is through asymmetric synthesis using ω-transaminase. However, reaching high yields often requires a strategy for shifting the equilibrium position. This paper describes a novel strategy for handling this problem. It involves the use of a supported liquid membrane (SLM) together with a packed bed reactor. The reactor contains Escherichia coli cells with ω-transaminase from Arthrobacter citreus, immobilized by flocculation with chitosan. The SLM consists of a hollow fibre membrane contactor in which the pores contain undecane. The system enables continuous extraction of the amine product and was used to successfully shift the equilibrium in asymmetric synthesis of (S)-α-methylbenzylamine (MBA). A conversion of 98% was reached, compared to 50% without product extraction. Moreover, a selective extraction of the produced MBA was realized. A high product concentration of 55g/l was reached after 80h, and the system showed promising potential for continuous operation.
authors.affiliation: Lund University, Department of Biotechnology, P.O. Box 124, 221 00 Lund, Sweden. Electronic address: Gustav.rehn@biotek.lu.se.
authors.forename: Gustav
authors.initials: G
authors.lastname: Rehn
authors.affiliation: Lund University, Department of Biotechnology, P.O. Box 124, 221 00 Lund, Sweden. Electronic address: Patrick.adlercreutz@biotek.lu.se.
authors.forename: Patrick
authors.initials: P
authors.lastname: Adlercreutz
authors.affiliation: Lund University, Department of Biotechnology, P.O. Box 124, 221 00 Lund, Sweden. Electronic address: Carl.grey@biotek.lu.se.
authors.forename: Carl
authors.initials: C
authors.lastname: Grey
mesh_terms: D000098:Acetophenones / Q000378:metabolism*; D000588:Amines / Q000302:isolation & purification* / Q000378:metabolism; D001173:Arthrobacter / Q000201:enzymology; D001426:Bacterial Proteins / Q000378:metabolism; D057005:Bioengineering / Q000379:methods*; D019149:Bioreactors; D048271:Chitosan / Q000378:metabolism; D008956:Models, Chemical; D012997:Solvents; D000637:Transaminases / Q000378:metabolism*
chemical_list: D000098:Acetophenones; D000588:Amines; D001426:Bacterial Proteins; D012997:Solvents; D048271:Chitosan; D000637:Transaminases
keywords: Asymmetric synthesis; Chiral amines; In situ product removal; Supported liquid membrane; ω-Transaminase