title: Use of thiolated oligonucleotides as anti-fouling diluents in electrochemical peptide-based sensors.
abstract: We incorporated short thiolated oligonucleotides as passivating diluents in the fabrication of electrochemical peptide-based (E-PB) sensors, with the goal of creating a negatively charged layer capable of resisting non-specific adsorption of matrix contaminants. The E-PB HIV sensors fabricated using these diluents were found to be more specific and selective, while retaining attributes similar to the sensor fabricated without these diluents. Overall, these results highlight the advantages of using oligonucleotides as anti-fouling diluents in self-assembled monolayer-based sensors.
authors.affiliation: Department of Chemistry, Hamilton Hall, University of Nebraska-Lincoln, Lincoln, NE 68588-0304, USA. rlai2@unl.edu.
authors.forename: Adam
authors.initials: A
authors.lastname: McQuistan
authors.forename: Anita J
authors.initials: AJ
authors.lastname: Zaitouna
authors.forename: Elena
authors.initials: E
authors.lastname: Echeverria
authors.forename: Rebecca Y
authors.initials: RY
authors.lastname: Lai
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000032:analysis*; D004563:Electrochemistry / Q000379:methods*; D009841:Oligonucleotides / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000737:chemistry*
chemical_list: D009841:Oligonucleotides; D013438:Sulfhydryl Compounds; D004247:DNA