title: Genome-wide analysis of water-stress-responsive microRNA expression profile in tobacco roots.
abstract: MicroRNAs (miRNAs) play a pivotal role in post-transcriptional regulation of gene expression in plants. In this study, we investigate miRNAs in an agronomically important common tobacco in China, named Honghua Dajinyuan (a drought-tolerant cultivar). Here, we report a comprehensive analysis of miRNA expression profiles in mock-treat grown (CK) and 20 % polyethylene glycol-grown (PEG-grown) tobacco roots using a high-throughput sequencing approach. A total of 656 unique miRNAs representing 53 miRNA families were identified in the two libraries, of which 286 unique miRNAs representing 162 microRNAs were differentially expressed. In addition, nine differentially expressed microRNAs selected from different expressed miRNA family with high abundance were subjected to further analysis and validated by quantitative real-time PCR (Q-PCR). In addition, the expression pattern of these identified candidate conserved miRNA and target genes of three identified miRNA (nta-miR172b, nta-miR156i, and nta-miR160a) were also validated by Q-PCR. Gene ontology (GO) enrichment analysis suggests that the putative target genes of these differentially expressed miRNAs are involved in metabolic process and response to stimulus. In particular, 25 target genes are involved in regulating plant hormone signal transduction and metabolism, indicating that these association microRNAs may play important regulatory roles in responding to PEG resistance. Moreover, this study adds a significant number of novel miRNAs to the tobacco miRNome.
authors.affiliation: Maize Research Institute of Sichuan Agricultural University/Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture, Chengdu, 611130, China.
authors.forename: Fuqiang
authors.initials: F
authors.lastname: Yin
authors.forename: Jian
authors.initials: J
authors.lastname: Gao
authors.forename: Ming
authors.initials: M
authors.lastname: Liu
authors.forename: Cheng
authors.initials: C
authors.lastname: Qin
authors.forename: Wenyou
authors.initials: W
authors.lastname: Zhang
authors.forename: Aiguo
authors.initials: A
authors.lastname: Yang
authors.forename: Mingzhong
authors.initials: M
authors.lastname: Xia
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Zhang
authors.forename: Yaou
authors.initials: Y
authors.lastname: Shen
authors.forename: Haijian
authors.initials: H
authors.lastname: Lin
authors.forename: Chenggang
authors.initials: C
authors.lastname: Luo
authors.forename: Guangtang
authors.initials: G
authors.lastname: Pan
mesh_terms: D003681:Dehydration; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D018745:Genome, Plant; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D010937:Plant Growth Regulators / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D011092:Polyethylene Glycols / Q000494:pharmacology; D018749:RNA, Plant / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction; D013312:Stress, Physiological; D014026:Nicotiana / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism
chemical_list: D035683:MicroRNAs; D010937:Plant Growth Regulators; D010940:Plant Proteins; D018749:RNA, Plant; D011092:Polyethylene Glycols