title: In vitro screening of antifungal compounds able to counteract biofilm development.
abstract: Fungi are able to grow as a single-species or a more complex biofilm attached to inert surfaces (catheters…) or tissues (lung…). This last form is a microbial niche which must be considered as a major risk factor of developing a human fungal infection. Nowadays, only a few therapeutic agents have been shown to be active against fungal biofilms in vitro and/or in vivo. So there is a real need to find new anti-biofilm molecules. Here we describe in detail some rapid, 96-well microtiter plate-based methods, for the screening of compounds with anti-biofilm activity against Candida spp. yeasts. Two approaches will be considered: prophylactic or curative effects of the tested compounds by producing biofilms on two supports - polystyrene well surfaces and catheter sections.
authors.affiliation: Laboratory of ecology and biology of the interactions, Faculty of Medicine Pharmacy, University of Poitiers, UMR CNRS 7267, 6, rue de la Miletrie - BP 199, 86034, Poitiers, France.
authors.forename: Marion
authors.initials: M
authors.lastname: Girardot
authors.forename: Christine
authors.initials: C
authors.lastname: Imbert
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D002175:Candida / Q000187:drug effects / Q000502:physiology; D004353:Drug Evaluation, Preclinical; D005658:Fungi / Q000187:drug effects* / Q000502:physiology*; D008826:Microbial Sensitivity Tests / Q000379:methods*
chemical_list: D000935:Antifungal Agents