title: Bone regeneration by the combined use of tetrapod-shaped calcium phosphate granules with basic fibroblast growth factor-binding ion complex gel in canine segmental radial defects.
abstract: The effect of tetrapod-shaped alpha tricalcium phosphate granules (Tetrabones(®) [TB]) in combination with basic fibroblast growth factor (bFGF)-binding ion complex gel (f-IC gel) on bone defect repair was examined. Bilateral segmental defects 20-mm long were created in the radius of 5 dogs, stabilized with a plate and screws and implanted with 1 of the following: TB (TB group), TB and bFGF solution (TB/f group), and TB and f-IC gel (TB/f-IC group). Dogs were euthanized 4 weeks after surgery. Radiographs showed well-placed TB granules in the defects and equal osseous callus formation in all the groups. Histomorphometry revealed that the number of vessels and volume of new bone in the TB/f-IC group were significantly higher than those in the other groups. However, no significant differences in neovascularization and new bone formation were observed between the TB/f and TB groups. Furthermore, no significant difference in the lamellar bone volume or rate of mineral apposition was observed among groups. These results suggest that increased bone formation might have been because of the promotion of neovascularization by the f-IC gel. Therefore, the combinatorial method may provide a suitable scaffold for bone regeneration in large segmental long bone defects.
authors.affiliation: Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Muneki
authors.initials: M
authors.lastname: Honnami
authors.forename: Sungjin
authors.initials: S
authors.lastname: Choi
authors.forename: I-li
authors.initials: IL
authors.lastname: Liu
authors.forename: Wataru
authors.initials: W
authors.lastname: Kamimura
authors.forename: Tetsushi
authors.initials: T
authors.lastname: Taguchi
authors.forename: Hironori
authors.initials: H
authors.lastname: Hojo
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Shimohata
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Ohba
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Koyama
authors.forename: Ryohei
authors.initials: R
authors.lastname: Nishimura
authors.forename: Ung-il
authors.initials: UI
authors.lastname: Chung
authors.forename: Nobuo
authors.initials: N
authors.lastname: Sasaki
authors.forename: Manabu
authors.initials: M
authors.lastname: Mochizuki
mesh_terms: D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects* / Q000502:physiology; D018786:Bone Substitutes; D002130:Calcium Phosphates / Q000737:chemistry*; D004285:Dogs; D016222:Fibroblast Growth Factor 2 / Q000737:chemistry / Q000378:metabolism*; D005782:Gels; D020007:Implants, Experimental; D008297:Male; D010012:Osteogenesis / Q000187:drug effects*; D011485:Protein Binding; D054457:Tissue Scaffolds; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D018786:Bone Substitutes; D002130:Calcium Phosphates; D005782:Gels; D016222:Fibroblast Growth Factor 2; C020243:calcium phosphate