title: Design and implementation of a microfluidic half adder chip based on double-stranded DNA.
abstract: In recent years, DNA computing has gained significant research interest. The design of a biochip with DNA computing as a carrier has become a key area in the development of a DNA molecular computer. The half adder, as the basic unit of various arithmetic units, has a complex structure that directly affects the overall complexity of a computer's structure. In this study, a half adder on a microfluidic chip is developed by means of bio-reaction. This technology is combined with a biochip and adopts glass and polydimethylsiloxane to fabricate a microscale hybrid chip. Using a DNA strand as an operand, realization of the half adder on a microfluidic chip is achieved by controlling the annealing and denaturation of double-stranded DNA. The computing results are rapidly and accurately obtained by detecting the presence of double-stranded DNA in a solution by agarose gel electrophoresis. The microfluidic half-adder chip accurately realizes half-adder computations and overcomes the shortcomings of traditional integrated circuit half adders, optical half adders, and chemical molecule half adders, such as complex structure, limited component size, and low accuracy.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Yourui
authors.initials: Y
authors.lastname: Huang
mesh_terms: D039301:Computers, Molecular; D004247:DNA; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D004566:Electrodes; D004867:Equipment Design; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods
chemical_list: D004129:Dimethylpolysiloxanes; C013830:baysilon; D004247:DNA