title: Tools and methods for studying the Drosophila JAK/STAT pathway.
abstract: The Janus Kinase/Signal Transducers and Activators of Transcription (JAK/STAT) signaling pathway is one of a limited number of signaling cascades that is extensively utilized for many developmental and homeostatic functions. The JAK/STAT pathway is evolutionarily conserved from insects to mammals, with homologous transduction machinery in each. Yet the mammalian pathway is composed of multiple members for each family of proteins, while flies have only a single homologue of most pathway components. This simplicity and the abundance of genetic, biochemical, and developmental tools make Drosophila an attractive model to investigate this important signaling pathway. This review provides a basic description of the Drosophila JAK/STAT cascade and summarizes currently available reagents and tools to study and manipulate the pathway.
authors.affiliation: University of Kentucky, Biology Dept., 101 T.H. Morgan Bldg., 675 Rose St., Lexington, KY 40506, USA.
authors.forename: Qian
authors.initials: Q
authors.lastname: Chen
authors.affiliation: University of Kentucky, Biology Dept., 101 T.H. Morgan Bldg., 675 Rose St., Lexington, KY 40506, USA.
authors.forename: Michelle
authors.initials: M
authors.lastname: Giedt
authors.affiliation: University of Kentucky, Biology Dept., 101 T.H. Morgan Bldg., 675 Rose St., Lexington, KY 40506, USA.
authors.forename: Lingfeng
authors.initials: L
authors.lastname: Tang
authors.affiliation: University of Kentucky, Biology Dept., 101 T.H. Morgan Bldg., 675 Rose St., Lexington, KY 40506, USA. Electronic address: DougH@uky.edu.
authors.forename: Douglas A
authors.initials: DA
authors.lastname: Harrison
mesh_terms: D000818:Animals; D004330:Drosophila / Q000235:genetics / Q000254:growth & development; D018507:Gene Expression Regulation, Developmental; D053612:Janus Kinases / Q000235:genetics / Q000378:metabolism*; D008967:Molecular Biology / Q000379:methods; D050791:STAT Transcription Factors / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000235:genetics*
chemical_list: D050791:STAT Transcription Factors; D053612:Janus Kinases
keywords: Cell signaling; Cytokine; Drosophila; Genetics; Janus kinase (JAK); STAT