title: Retrograde labeling of corticospinal motor neurons from early postnatal rodents.
abstract: Immunopanning of viable neurons requires availability of an antibody directed against a neuronal surface epitope that is specific for a neuron type of interest (e.g., corticospinal motor neurons [CSMNs]) and that is able to immobilize only neurons of interest from a dissociated brain suspension on a culture dish. However, few, if any, neuron type-specific monoclonal antibodies have been developed. Panning antibodies have not been characterized for CSMNs. To circumvent this issue, we developed a method to retrograde label CSMNs with a cholera toxin B subunit (CTB) tracer that localizes to the plasma membrane only of labeled CSMNs. The main objective of this procedure is to provide CSMNs with a surface marker that can be used to immunopan only the labeled CSMNs by using a CTB-specific antibody.
authors.affiliation: Laboratory for the Research of Neurodegenerative Diseases, VIB Center for the Biology of Disease, KU Leuven Center for Human Genetics, 3000 Leuven, Belgium.
authors.forename: Wim
authors.initials: W
authors.lastname: Mandemakers
mesh_terms: D000818:Animals; D000992:Antitoxins / Q000378:metabolism; D002469:Cell Separation / Q000379:methods*; D002540:Cerebral Cortex / Q000166:cytology*; D002772:Cholera Toxin / Q000378:metabolism; D007158:Immunologic Techniques / Q000379:methods*; D009046:Motor Neurons / Q000502:physiology*; D051381:Rats; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D000992:Antitoxins; D002772:Cholera Toxin