title: [Therapeutic effect of fibroblast growth factor 21 on NAFLD in MSG-iR mice and its mechanism].
abstract: This study is to evaluate the therapeutic effect of fibroblast growth factor 21 (FGF21) on NAFLD in MSG-IR mice and to provide mechanism insights into its therapeutic effect. The MSG-IR mice with insulin resistance were treated with high dose (0.1 micromol.kg-1d-1) and low dose (0.025 micromol.kg-1d-1) of FGF21 once a day for 5 weeks. Body weight was measured weekly. At the end of the experiment, serum lipids, insulin and aminotransferases were measured. Hepatic steatosis was observed. The expression of key genes regulating energy metabolism were detected by real-time PCR. The results showed that after 5 weeks treatment, both doses of FGF21 reduced body weight (P<0.01), corrected dyslipidemia (P<0.01), reversed steatosis and restored the liver morphology in the MSG model mice and significantly ameliorated insulin resistance. Additionally, real-time PCR showed that FGF21 significantly reduced transcription levels of fat synthetic genes, decreased fat synthesis and promoted lipolysis and energy metabolism by up-regulating key genes of lipolysis, thereby liver fat accumulation was reduced and liver function was restored to normal levels. In conclusion, FGF21 significantly reduces body weight of the MSG-IR mice, ameliorates insulin resistance, reverses hepatic steatosis. These findings provide a theoretical support for clinical application of FGF21 as a novel therapeutics for treatment of NAFLD.
authors.forename: Sheng-Long
authors.initials: SL
authors.lastname: Zhu
authors.forename: Zhen-Yu
authors.initials: ZY
authors.lastname: Zhang
authors.forename: Gui-Ping
authors.initials: GP
authors.lastname: Ren
authors.forename: Xian-Long
authors.initials: XL
authors.lastname: Ye
authors.forename: Lei
authors.initials: L
authors.lastname: Ma
authors.forename: Dan
authors.initials: D
authors.lastname: Yu
authors.forename: Miao-Miao
authors.initials: MM
authors.lastname: Han
authors.forename: Jing-Zhuang
authors.initials: JZ
authors.lastname: Zhao
authors.forename: Tian-Yuan
authors.initials: TY
authors.lastname: Zhang
authors.forename: De-Shan
authors.initials: DS
authors.lastname: Li
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D050171:Dyslipidemias / Q000378:metabolism; D004734:Energy Metabolism / Q000187:drug effects; D005234:Fatty Liver / Q000139:chemically induced / Q000150:complications; D005260:Female; D005346:Fibroblast Growth Factors / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D007333:Insulin Resistance; D008066:Lipolysis / Q000187:drug effects; D008099:Liver / Q000378:metabolism / Q000473:pathology*; D008297:Male; D051379:Mice; D065626:Non-alcoholic Fatty Liver Disease / Q000188:drug therapy*; D012970:Sodium Glutamate
chemical_list: C414620:fibroblast growth factor 21; D005346:Fibroblast Growth Factors; D012970:Sodium Glutamate