title: MicroRNA-155 in oral squamous cell carcinoma: Overexpression, localization, and prognostic potential.
abstract: BACKGROUND
  Oral squamous cell carcinoma (OSCC) is a common malignancy with poor prognosis. MicroRNAs (miRNAs) play an important role in cancer, but their role in OSCC is not clarified.
  
  
  METHODS
  We performed miRNA microarray, quantitative reverse transcription polymerase chain reaction (qRT-PCR), and fluorescence in situ hybridization (FISH) to examine miRNA expression in OSCC and paired adjacent cancer-free mucosal (ACF) tissues.
  
  
  RESULTS
  Thirteen miRNAs, including miRNA-155, were upregulated (>2-fold) in OSCC against ACF. MiRNA-155 was confirmed to have significantly higher expression in OSCC against ACF (paired-samples t test; p = .041) and it was localized in the cancer nest, inflammatory area, and vascular endothelium of OSCC. High expression of miRNA-155 in ACF tissue was an independent prognostic indicator for OSCC survival.
  
  
  CONCLUSIONS
  MiRNA-155 was overexpressed in OSCC and it was located in the cancer nest, inflammatory area, and vascular endothelium of OSCC. High miRNA-155 expression level in ACF may predict poor prognosis in patients with OSCC.
authors.affiliation: Department of Oral Mucosal Diseases, Shanghai Ninth People's Hospital, Shanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Lin-Jun
authors.initials: LJ
authors.lastname: Shi
authors.affiliation: Department of Oral Mucosal Diseases, Shanghai Ninth People's Hospital, Shanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Chen-Yang
authors.initials: CY
authors.lastname: Zhang
authors.affiliation: Department of Oral Mucosal Diseases, Shanghai Ninth People's Hospital, Shanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Zeng-Tong
authors.initials: ZT
authors.lastname: Zhou
authors.affiliation: Department of Oral Mucosal Diseases, Shanghai Ninth People's Hospital, Shanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Jing-Yuan
authors.initials: JY
authors.lastname: Ma
authors.affiliation: Department of Oral Mucosal Diseases, Shanghai Ninth People's Hospital, Shanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Oral Mucosal Diseases, Shanghai Ninth People's Hospital, Shanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Zhe-Xuan
authors.initials: ZX
authors.lastname: Bao
authors.affiliation: Department of Oral Mucosal Diseases, Shanghai Ninth People's Hospital, Shanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Wei-Wen
authors.initials: WW
authors.lastname: Jiang
mesh_terms: D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D046228:Microarray Analysis; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D011379:Prognosis; D060888:Real-Time Polymerase Chain Reaction
chemical_list: C524942:MIRN155 microRNA, human; D035683:MicroRNAs
keywords: fluorescence in situ hybridization; microRNA-155; oral squamous cell carcinoma; polymerase chain reaction (PCR); prognosis