title: RNA and β-hemolysin of group B Streptococcus induce interleukin-1β (IL-1β) by activating NLRP3 inflammasomes in mouse macrophages.
abstract: The inflammatory cytokine IL-1β is critical for host responses against many human pathogens. Here, we define Group B Streptococcus (GBS)-mediated activation of the Nod-like receptor-P3 (NLRP3) inflammasome in macrophages. NLRP3 activation requires GBS expression of the cytolytic toxin, β-hemolysin, lysosomal acidification, and leakage. These processes allow the interaction of GBS RNA with cytosolic NLRP3. The present study supports a model in which GBS RNA, along with lysosomal components including cathepsins, leaks out of lysosomes and interacts with NLRP3 to induce IL-1β production.
authors.affiliation: From the Division of Infectious Diseases and Immunology, University of Massachusetts Medical School, Worcester, Massachusetts 01605.
authors.forename: Rahul
authors.initials: R
authors.lastname: Gupta
authors.affiliation: From the Division of Infectious Diseases and Immunology, University of Massachusetts Medical School, Worcester, Massachusetts 01605.
authors.forename: Shubhendu
authors.initials: S
authors.lastname: Ghosh
authors.affiliation: From the Division of Infectious Diseases and Immunology, University of Massachusetts Medical School, Worcester, Massachusetts 01605.
authors.forename: Brian
authors.initials: B
authors.lastname: Monks
authors.affiliation: From the Division of Infectious Diseases and Immunology, University of Massachusetts Medical School, Worcester, Massachusetts 01605.
authors.forename: Rosane B
authors.initials: RB
authors.lastname: DeOliveira
authors.affiliation: From the Division of Infectious Diseases and Immunology, University of Massachusetts Medical School, Worcester, Massachusetts 01605.
authors.forename: Te-Chen
authors.initials: TC
authors.lastname: Tzeng
authors.affiliation: From the Division of Infectious Diseases and Immunology, University of Massachusetts Medical School, Worcester, Massachusetts 01605.
authors.forename: Parisa
authors.initials: P
authors.lastname: Kalantari
authors.affiliation: From the Division of Infectious Diseases and Immunology, University of Massachusetts Medical School, Worcester, Massachusetts 01605.
authors.forename: Anubhab
authors.initials: A
authors.lastname: Nandy
authors.affiliation: From the Division of Infectious Diseases and Immunology, University of Massachusetts Medical School, Worcester, Massachusetts 01605.
authors.forename: Bornali
authors.initials: B
authors.lastname: Bhattacharjee
authors.affiliation: From the Division of Infectious Diseases and Immunology, University of Massachusetts Medical School, Worcester, Massachusetts 01605.
authors.forename: Jennie
authors.initials: J
authors.lastname: Chan
authors.affiliation: the Laboratório de Inflamação e Imunidade, Departamento de Imunologia, Instituto de Microbiologia, Universidade Federal do Rio de Janeiro (UFRJ), 21941-902 Rio de Janeiro, Brazil, and.
authors.forename: Fabianno
authors.initials: F
authors.lastname: Ferreira
authors.affiliation: From the Division of Infectious Diseases and Immunology, University of Massachusetts Medical School, Worcester, Massachusetts 01605.
authors.forename: Vijay
authors.initials: V
authors.lastname: Rathinam
authors.affiliation: From the Division of Infectious Diseases and Immunology, University of Massachusetts Medical School, Worcester, Massachusetts 01605.
authors.forename: Shruti
authors.initials: S
authors.lastname: Sharma
authors.affiliation: From the Division of Infectious Diseases and Immunology, University of Massachusetts Medical School, Worcester, Massachusetts 01605.
authors.forename: Egil
authors.initials: E
authors.lastname: Lien
authors.affiliation: From the Division of Infectious Diseases and Immunology, University of Massachusetts Medical School, Worcester, Massachusetts 01605.
authors.forename: Neal
authors.initials: N
authors.lastname: Silverman
authors.affiliation: From the Division of Infectious Diseases and Immunology, University of Massachusetts Medical School, Worcester, Massachusetts 01605.
authors.forename: Katherine
authors.initials: K
authors.lastname: Fitzgerald
authors.affiliation: the Institut Pasteur, Unité de Biologie des Bactéries Pathogènes à Gram-Positif, 75724 Paris Cedex 15, France.
authors.forename: Arnaud
authors.initials: A
authors.lastname: Firon
authors.affiliation: the Institut Pasteur, Unité de Biologie des Bactéries Pathogènes à Gram-Positif, 75724 Paris Cedex 15, France.
authors.forename: Patrick
authors.initials: P
authors.lastname: Trieu-Cuot
authors.affiliation: the Center of Chronic Immunodeficiency and Center of Pediatrics and Adolescent Medicine, University Medical Center Freiburg, 79106 Freiburg im Breisgau, Germany.
authors.forename: Philipp
authors.initials: P
authors.lastname: Henneke
authors.affiliation: From the Division of Infectious Diseases and Immunology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, Douglas.Golenbock@umassmed.edu.
authors.forename: Douglas T
authors.initials: DT
authors.lastname: Golenbock
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D006460:Hemolysin Proteins / Q000378:metabolism*; D006801:Humans; D058847:Inflammasomes / Q000378:metabolism*; D053583:Interleukin-1beta / Q000096:biosynthesis* / Q000378:metabolism; D008247:Lysosomes / Q000378:metabolism / Q000382:microbiology; D008264:Macrophages / Q000166:cytology / Q000378:metabolism* / Q000382:microbiology; D051379:Mice; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; D010588:Phagosomes / Q000378:metabolism / Q000382:microbiology; D012329:RNA, Bacterial / Q000378:metabolism*; D013292:Streptococcus agalactiae / Q000378:metabolism / Q000502:physiology*
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D006460:Hemolysin Proteins; D058847:Inflammasomes; D053583:Interleukin-1beta; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; C453881:NLRP3 protein, human; C492716:Nlrp3 protein, mouse; D012329:RNA, Bacterial; C071495:streptococcal group B hemolysin
keywords: Cell Signaling; Immunology; Innate Immunity; Interleukin; RNA