title: Maotai ameliorates diethylnitrosamine-initiated hepatocellular carcinoma formation in mice.
abstract: Consumption of alcohol is closely related to liver disease, such as hepatic fibrosis or even hepatocellular carcinoma (HCC). However, epidemiological and experimental studies indicated that consumption of Maotai, one of the famous liquors in China, exhibits no significant correlation with hepatic fibrosis or cirrhosis as other beverage sources do. This study detected the relationship of Maotai consumption and HCC development in a diethylnitrosamine (DEN)-initiated HCC animal model. DEN was given to mice at a dose of 100 mg/kg, ip, and 50 mg/kg, ip in the following week. Mice were simultaneously given Maotai or an equal amount of ethanol (53%, 5 ml/kg/day, 5 days/week for up to 35 weeks). At 3-week and 35- week of the experiment, serum and livers were collected for biochemical and histopathological examination of liver injury and incidence of HCC. Real-time RT-PCR, immunohistochemistry and Western blotting were used to examine the expression of metallothionein-1/2 (MT-1/2), NF-E2-related factor 2 (Nrf2), glutamate-cysteine ligase catalytic subunit (GCLC) and modified subunit (GCLM). We identified tissue damage and dysfunction of liver in ethanol + DEN-treated mice, whereas the extent of injury was reduced in Maotai+ DEN -treated mice. Significant Glypican-3(GPC3) expression and precancerous injury or HCC were seen in approximately 50% of mice with ethanol+ DEN, but barely be seen in Maotai + DEN-treated mice. A higher expression of MT-1/2, Nrf2 and GCLC could be seen in Maotai + DEN-treated mice. Thus, Maotai liquor ameliorates the formation of DEN-induced HCC in mice, and the protection mechanism is possibly related with the activation of anti-oxidation factors, such as MTs, Nrf2 and GCLC.
authors.affiliation: Guiyang Medical College Hospital, GuiYang, Guizhou, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Yi
authors.affiliation: Guiyang Medical College Hospital, GuiYang, Guizhou, China.
authors.forename: Li
authors.initials: L
authors.lastname: Long
authors.affiliation: Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland, United States of America.
authors.forename: Chunzhang
authors.initials: C
authors.lastname: Yang
authors.affiliation: Center for Therapeutic Research of Hepatocellular Carcinoma, Beijing 302 Hospital, Beijing, China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Guiyang Medical College Hospital, GuiYang, Guizhou, China.
authors.forename: Mingliang
authors.initials: M
authors.lastname: Cheng
mesh_terms: D000434:Alcoholic Beverages / Q000009:adverse effects*; D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D002681:China; D004052:Diethylnitrosamine / Q000633:toxicity; D004195:Disease Models, Animal; D000431:Ethanol / Q000008:administration & dosage / Q000009:adverse effects; D005355:Fibrosis / Q000139:chemically induced / Q000473:pathology*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008113:Liver Neoplasms / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D051379:Mice; D051267:NF-E2-Related Factor 2 / Q000096:biosynthesis
chemical_list: D051267:NF-E2-Related Factor 2; C495636:Nfe2l2 protein, mouse; D004052:Diethylnitrosamine; D000431:Ethanol