title: Isolation and propagation of neural stem cells in caprine (Capra hircus).
abstract: Neural stem cells (NSCs) can self-renew and give rise to neurons, astrocytes and oligodendrocytes; they are found in the nervous system of mammalian organisms, representing a promising resource for both fundamental research and therapeutics. There have been few investigations on NSCs in the livestock species. Therefore, we have successfully isolated and characterised NSCs from the foetal brain of a small domestic animal, the goat (called GNSCs). These cells from the foetal brain showed self-renewal, rapid proliferation with a population doubling time of 88 h, were morphologically homogeneous and maintained normal chromosome throughout the culture period. The cells expressed NSC-specific markers (Sox2, Pax6 and Mushashi), but were negative for CD34 and CD45. They were capable of multi-differentiation into neurons, astrocytes, oligodendrocytes, as well as adipocytes and osteocytes. The availability of such cells may hold great interest for basic and applied neuroscience.
authors.affiliation: Reproductive Physiology Lab, Division of Physiology & Climatology, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, 243122, India.
authors.forename: Pranjali
authors.initials: P
authors.lastname: Agarwal
authors.forename: Manish
authors.initials: M
authors.lastname: Kumar
authors.forename: Kuldeep
authors.initials: K
authors.lastname: Kumar
authors.forename: Renu
authors.initials: R
authors.lastname: Singh
authors.forename: Puspendra Saswat
authors.initials: PS
authors.lastname: Mahapatra
authors.forename: Ajay
authors.initials: A
authors.lastname: Kumar
authors.forename: Sanjeev Kumar
authors.initials: SK
authors.lastname: Bhure
authors.forename: Dhruba
authors.initials: D
authors.lastname: Malakar
authors.forename: Mihir
authors.initials: M
authors.lastname: Sarkar
authors.forename: Sadhan
authors.initials: S
authors.lastname: Bag
mesh_terms: D000818:Animals; D000943:Antigens, Differentiation / Q000378:metabolism; D002454:Cell Differentiation; D049109:Cell Proliferation; D002469:Cell Separation; D002478:Cells, Cultured; D006041:Goats; D058953:Neural Stem Cells / Q000502:physiology*
chemical_list: D000943:Antigens, Differentiation
keywords: NSC; characterisation; differentiation; goat/caprine; isolation