title: The protective effects of pomegranate extracts against renal ischemia-reperfusion injury in male rats.
abstract: OBJECTIVE
  To evaluate the possible protective effect of pomegranate extract (PE) on rats following renal ischemia-reperfusion (I/R) injury.
  
  
  METHODS
  Twenty-four Wistar rats were divided into three groups. Sham group underwent laparotomy then waited for 45 minutes without ischemia. I/R group were subjected to left renal ischemia for 45 minutes followed by 60 minutes of reperfusion. I/R + PE group were subjected to the same renal I/R as the I/R group were also given 225 mg/kg PE peroral 30 minutes prior to the ischemia. Malondialdehyde (MDA), total antioxidant capacity (TAC), total oxidant status (TOS), and oxidative stress index (OSI) were determined on the blood samples and kidney tissues. Histopathological analyses were conducted on the kidney tissues.
  
  
  RESULTS
  Serum TAC levels were significantly decreased in I/R group when compared with S group (P = 0.001). Serum MDA levels were increased in I/R group; however, it was not statistically significant. In rat kidney tissues, TOS levels and OSI index were significantly increased after I/R injury, while TAC levels were decreased. In I/R + PE group, PE reversed the negative effects of I/R injury. PE pretreatment was effective in decreasing tubular necrosis score.
  
  
  CONCLUSIONS
  PE pretreatment ameliorated the oxidative damage and histopathological changes occurring following renal I/R injury.
authors.affiliation: Department of Urology, Dicle University, Diyarbakır, Turkey.
authors.forename: Ahmet A
authors.initials: AA
authors.lastname: Sancaktutar
authors.affiliation: Department of Urology, Dicle University, Diyarbakır, Turkey.
authors.forename: Mehmet N
authors.initials: MN
authors.lastname: Bodakci
authors.affiliation: Department of Urology, Dicle University, Diyarbakır, Turkey.
authors.forename: Namık K
authors.initials: NK
authors.lastname: Hatipoglu
authors.affiliation: Department of Urology, Dicle University, Diyarbakır, Turkey.
authors.forename: Haluk
authors.initials: H
authors.lastname: Soylemez
authors.affiliation: Department of Biochemistry, Dicle University, Diyarbakır, Turkey.
authors.forename: Kemal
authors.initials: K
authors.lastname: Basarılı
authors.affiliation: Department of Pathology, Faculty of Medicine, Dicle University, Diyarbakır, Turkey.
authors.forename: Gul
authors.initials: G
authors.lastname: Turkcu
keywords: Antioxidant; ischemia-reperfusion; kidney; pomegranate extract; rat