title: Down-regulation of Dicer expression in cervical cancer tissues.
abstract: Dicer is a component of the MicroRNA-producing machinery. The altered expression of Dicer may play a role in oncogenesis. The purpose of this study was to evaluate the expression of Dicer in cervical cancer tissues and its significance. Cancer tissues, para-cancer tissues and corresponding normal tissues were obtained from 31 cervical cancer patients undergoing surgery. Expression levels of Dicer mRNA were evaluated in these tissues using the real-time reverse transcription PCR. The association of the Dicer expression levels with clinical characteristics was also examined. The expression levels of Dicer mRNA were decreased in 66.7% (18/27) and 76.9% (20/26) of cervical cancer and para-cancer specimens, respectively, compared with corresponding normal tissues. The lower expression levels of Dicer in cancer tissues were associated with advanced tumor stages (p = 0.03) and with metastasis (p = 0.01). The down-regulation of Dicer expression in cancer and para-cancer tissues was observed. The lower expression of Dicer in cancer tissues demonstrated to be associated with tumor stages and metastasis. Future studies with a greater number of tissues to more conclusively determine the extent of low-level expression of Dicer in cervical cancer are warranted.
authors.affiliation: Department of Gynecology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, China.
authors.forename: Hongqin
authors.initials: H
authors.lastname: Zhao
authors.forename: Xiance
authors.initials: X
authors.lastname: Jin
authors.forename: Huafang
authors.initials: H
authors.lastname: Su
authors.forename: Xia
authors.initials: X
authors.lastname: Deng
authors.forename: Ya
authors.initials: Y
authors.lastname: Fang
authors.forename: Lanxiao
authors.initials: L
authors.lastname: Shen
authors.forename: Congying
authors.initials: C
authors.lastname: Xie
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics* / Q000378:metabolism; D015153:Blotting, Western; D016022:Case-Control Studies; D002584:Cervix Uteri / Q000378:metabolism*; D053487:DEAD-box RNA Helicases / Q000235:genetics* / Q000378:metabolism; D015536:Down-Regulation; D005260:Female; D005500:Follow-Up Studies; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D011446:Prospective Studies; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D043244:Ribonuclease III / Q000235:genetics* / Q000378:metabolism; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; D012333:RNA, Messenger; C408087:DICER1 protein, human; D043244:Ribonuclease III; D053487:DEAD-box RNA Helicases