title: Using fluorescence correlation spectroscopy (FCS) for IFN-g detection: a preliminary study.
abstract: Nowadays, enzyme-linked immunosorbent assay (ELISA) based detection of Mycobacterium tuberculosis (M. tuberculosis) antigen triggered interferon-gamma (IFN-g) secretion by blood T cells displays an improved diagnostic value for M. tuberculosis infection. Applications of fluorescence correlation spectroscopy (FCS) have been explored in various subfields of medicine and molecular biology, including detection of a certain biomarker in liquid instead of ELISA. Here, we present a preliminary study of detecting IFN-g using FCS-based technique.
authors.affiliation: Key Laboratory of Major Diseases in Children and National, Key Discipline of Pediatrics (Capital Medical University), Ministry of Education, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, Beijing 100045, China. Electronic address: shenchen1110@bjmu.edu.cn.
authors.forename: Chen
authors.initials: C
authors.lastname: Shen
authors.affiliation: Max F. Perutz Laboratories, Department of Structural Biology and Biomolecular Chemistry, University of Vienna, Vienna, Austria; OnkoTec Waidhofen/Thaya, Vienna, Austria.
authors.forename: Martin
authors.initials: M
authors.lastname: Knapp
authors.affiliation: Max F. Perutz Laboratories, Department of Structural Biology and Biomolecular Chemistry, University of Vienna, Vienna, Austria; OnkoTec Waidhofen/Thaya, Vienna, Austria.
authors.forename: Martin Gerald
authors.initials: MG
authors.lastname: Puchinger
authors.affiliation: Max F. Perutz Laboratories, Department of Structural Biology and Biomolecular Chemistry, University of Vienna, Vienna, Austria; OnkoTec Waidhofen/Thaya, Vienna, Austria.
authors.forename: Aamir
authors.initials: A
authors.lastname: Shahzad
authors.affiliation: Max F. Perutz Laboratories, Department of Structural Biology and Biomolecular Chemistry, University of Vienna, Vienna, Austria; OnkoTec Waidhofen/Thaya, Vienna, Austria.
authors.forename: Erwin
authors.initials: E
authors.lastname: Gaubitzer
authors.affiliation: Key Laboratory of Major Diseases in Children and National, Key Discipline of Pediatrics (Capital Medical University), Ministry of Education, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, Beijing 100045, China.
authors.forename: A Dong
authors.initials: AD
authors.lastname: Shen
authors.affiliation: Max F. Perutz Laboratories, Department of Structural Biology and Biomolecular Chemistry, University of Vienna, Vienna, Austria; OnkoTec Waidhofen/Thaya, Vienna, Austria.
authors.forename: Gottfried
authors.initials: G
authors.lastname: Koehler
mesh_terms: D000942:Antigens, Bacterial / Q000276:immunology; D015415:Biomarkers / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007371:Interferon-gamma / Q000032:analysis*; D008213:Lymphocyte Activation; D009169:Mycobacterium tuberculosis / Q000276:immunology* / Q000302:isolation & purification*; D011237:Predictive Value of Tests; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013601:T-Lymphocytes / Q000276:immunology*; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis*
chemical_list: D000942:Antigens, Bacterial; D015415:Biomarkers; D007371:Interferon-gamma
keywords: Diagnostic; Fluorescence correlation spectroscopy; Interferon-gamma; Monoclonal antibodies