title: Early diagnosis of Irkut virus infection using magnetic bead-based serum peptide profiling by MALDI-TOF MS in a mouse model.
abstract: Early diagnosis is important for the prompt post-exposure prophylaxis of lyssavirus infections. To diagnose Irkut virus (IRKV) infection during incubation in mice, a novel method using magnetic bead-based serum peptide profiling by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been established. For this test, serum peptides were concentrated by adsorption to and elution from the magnetic bead-based weak cation ion exchanger. Mass spectrograms obtained by MALDI-TOF MS were analyzed using ClinProTools bioinformatics software. Construction of the diagnostic model was performed using serum samples from mice infected with IRKV and rabies virus (RABV) BD06, Flury-LEP, and SRV9 (as controls). The method accurately diagnosed sera 2, 4 and 8 days after IRKV and RABV infections. The sensitivity, specificity, and total accuracy of diagnosis were 86.7%, 95.2%, and 92.9%, respectively. However, IRKV could not be differentiated from RABV 1 day after infection. The results of the present study indicate that serum peptide profiling by MALDI-TOF MS is a promising technique for the early clinical diagnosis of lyssavirus infections and needs to be further tested in humans and farm animals.
authors.affiliation: Military Veterinary Research Institute, Academy of Military Medical Sciences, 666 Liuying West Road, Jingyue Economic Development Zone, Changchun 130122, China. linan226@126.com.
authors.forename: Nan
authors.initials: N
authors.lastname: Li
authors.affiliation: Military Veterinary Research Institute, Academy of Military Medical Sciences, 666 Liuying West Road, Jingyue Economic Development Zone, Changchun 130122, China. liuye79@126.com.
authors.forename: Ye
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Military Veterinary Research Institute, Academy of Military Medical Sciences, 666 Liuying West Road, Jingyue Economic Development Zone, Changchun 130122, China. hao1107@126.com.
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Hao
authors.affiliation: Military Veterinary Research Institute, Academy of Military Medical Sciences, 666 Liuying West Road, Jingyue Economic Development Zone, Changchun 130122, China. zhangshoufeng@hotmail.com.
authors.forename: Shoufeng
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Military Veterinary Research Institute, Academy of Military Medical Sciences, 666 Liuying West Road, Jingyue Economic Development Zone, Changchun 130122, China. ronglianghu@hotmail.com.
authors.forename: Rongliang
authors.initials: R
authors.lastname: Hu
authors.affiliation: Military Veterinary Research Institute, Academy of Military Medical Sciences, 666 Liuying West Road, Jingyue Economic Development Zone, Changchun 130122, China. jipingli85981@126.com.
authors.forename: Jiping
authors.initials: J
authors.lastname: Li
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood; D002460:Cell Line; D006224:Cricetinae; D004195:Disease Models, Animal; D042241:Early Diagnosis; D005260:Female; D018114:Lyssavirus / Q000145:classification*; D008280:Magnetics / Q000379:methods*; D058185:Magnetite Nanoparticles; D051379:Mice; D008807:Mice, Inbred BALB C; D018353:Rhabdoviridae Infections / Q000175:diagnosis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014764:Viral Proteins / Q000097:blood*
chemical_list: D015415:Biomarkers; D058185:Magnetite Nanoparticles; D014764:Viral Proteins