title: miR-137 controls proliferation and differentiation of human adipose tissue stromal cells.
abstract: OBJECTIVE
  Demonstrating the molecular mechanisms of human adipose tissue-derived mesenchymal stem cells (hADSCs) differentiation and proliferation could develop hADSCs-based cell therapy.
  
  
  METHODS
  The microRNA-137 (miR-137) and cell division control protein 42 homolog (CDC42) levels were regulated by oligonucleotides transfection. The adipogenic differentiation was induced for 10 days in an adipogenic medium and assessed by using an Oil Red O stain. The regulation of miR-137 on CDC42 expression was determined by western blot, real-time PCR and luciferase reporter assay.
  
  
  RESULTS
  We confirmed the roles of miR-137 on hADSCs proliferation and adipogenic differentiation. We showed that overexpression of miR-137 inhibited both hADSCs proliferation and adipogenic differentiation. Overexpression of miR-137 also downregulated protein and mRNA levels of CDC42, a predicted target of miR-137. In contrast, inhibition of miR-137 with 2'-O-methyl antisense RNA increased proliferation and adipogenic differentiation in hADSCs. Luciferase reporter activity in the miR-137 target site within the CDC42 3'UTR was lower in miR-137-transfected hADSCs than in control miRNA-transfected hADSCs. RNA interference-mediated downregulation of CDC42 in hADSCs inhibited their proliferation and adipogenic differentiation.
  
  
  CONCLUSIONS
  Our results indicate that miR-137 regulates hADSCs adipogenic differentiation and proliferation by directly targeting CDC42. These findings improve our knowledge of the molecular mechanisms governing hADSCs differentiation and proliferation.
authors.affiliation: Department of Physiology, School of Medicine, Pusan National University, Pusan, Korea.
authors.forename: Keun Koo
authors.initials: KK
authors.lastname: Shin
authors.forename: Young Suk
authors.initials: YS
authors.lastname: Kim
authors.forename: Jee Young
authors.initials: JY
authors.lastname: Kim
authors.forename: Yong Chan
authors.initials: YC
authors.lastname: Bae
authors.forename: Jin Sup
authors.initials: JS
authors.lastname: Jung
mesh_terms: D050156:Adipogenesis / Q000502:physiology*; D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism*; D002454:Cell Differentiation / Q000502:physiology*; D049109:Cell Proliferation / Q000502:physiology*; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism; D020764:cdc42 GTP-Binding Protein / Q000096:biosynthesis*
chemical_list: C545567:MIRN137 microRNA, human; D035683:MicroRNAs; D020764:cdc42 GTP-Binding Protein