title: Biomanufacture of human platelets for transfusion: Rationale and approaches.
abstract: Platelets for transfusion obtained from volunteer blood donors are a limited resource. Given the increased range of donor restrictions to prevent transmission of disease and the decline in volunteer blood donors, there is a diminishing supply of blood for transfusion. Production of mature blood cells from hematopoietic stem cells via large-scale manufacture is an alternative way of meeting transfusion demands. In this review, we provide a detailed outline of the challenges and opportunities for the biomanufacture of platelets. We describe the scale required for platelet biomanufacture to deliver sufficient cells for transfusion, provide a brief outline of the current understanding of megakaryopoiesis and thrombogenesis, and highlight how the current understanding impacts the design of culture systems and bioreactors for producing platelets.
authors.affiliation: Materials Science and Engineering, Commonwealth Scientific Industrial Research Organisation, Victoria, Australia; Australian Regenerative Medicine Institute, Monash University, Victoria, Australia.
authors.forename: Eun-ju
authors.initials: EJ
authors.lastname: Lee
authors.affiliation: Materials Science and Engineering, Commonwealth Scientific Industrial Research Organisation, Victoria, Australia; Australian Regenerative Medicine Institute, Monash University, Victoria, Australia.
authors.forename: Pankaj
authors.initials: P
authors.lastname: Godara
authors.affiliation: Materials Science and Engineering, Commonwealth Scientific Industrial Research Organisation, Victoria, Australia; Australian Regenerative Medicine Institute, Monash University, Victoria, Australia. Electronic address: david.haylock@csiro.au.
authors.forename: David
authors.initials: D
authors.lastname: Haylock
mesh_terms: D001792:Blood Platelets; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D006412:Hematopoietic Stem Cells; D006801:Humans; D034061:Thrombopoiesis; D016212:Transforming Growth Factor beta
chemical_list: D016212:Transforming Growth Factor beta