title: Pharmacokinetics and liver distribution study of unbound curdione and curcumol in rats by microdialysis coupled with rapid resolution liquid chromatography (RRLC) and tandem mass spectrometry.
abstract: A sensitive, specific, convenient and endogenous interference-free microdialysis sampling method coupled with RRLC-MS was successfully developed and applied to the determination of protein-unbound curdione and curcumol in biological samples. Microdialysis probes were simultaneously inserted into the jugular vein toward heart and the median lobe near the center of liver of rats under anesthesia. The separation was accomplished on a Zorbax SB-C18 column (2.1 mm × 50 mm, 1.8 μm) with a gradient elution and chromatography was conducted with RRLC system. Analytes were detected by positive ion electrospray tandem mass spectrometry and quantified on the basis of extracted ion chromatography (EIC) peak area signal. The calibration curves were linear over the range of 3.3-213.2 ng/mL for curdione and 8.1-519.2 ng/mL for curcumol. All the validation data, such as accuracy, precision, stability and matrix effect were satisfactory and within the required limits. The validated method was successfully applied to the pharmacokinetic study of curdione and curcumol in rat blood and liver after oral administration of Rhizoma Curcumae extracts. The results could provide a meaningful basis for better understanding of the intracorporal process of Rhizoma Curcumae, which would be helpful for further study both in clinic and laboratory.
authors.affiliation: The Key Research Laboratory of Chinese Medicine Processing of Jiangsu Province, Nanjing University of Chinese Medicine, Nanjing, PR China.
authors.forename: Jinci
authors.initials: J
authors.lastname: Li
authors.affiliation: The Key Research Laboratory of Chinese Medicine Processing of Jiangsu Province, Nanjing University of Chinese Medicine, Nanjing, PR China.
authors.forename: Chunqin
authors.initials: C
authors.lastname: Mao
authors.affiliation: College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.affiliation: College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, PR China.
authors.forename: De
authors.initials: D
authors.lastname: Ji
authors.affiliation: College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, PR China.
authors.forename: Fangzhou
authors.initials: F
authors.lastname: Yin
authors.affiliation: College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, PR China.
authors.forename: Yongying
authors.initials: Y
authors.lastname: Lang
authors.affiliation: The Key Research Laboratory of Chinese Medicine Processing of Jiangsu Province, Nanjing University of Chinese Medicine, Nanjing, PR China; College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, PR China. Electronic address: lutuling2005@126.com.
authors.forename: Tulin
authors.initials: T
authors.lastname: Lu
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, PR China.
authors.forename: Yongqing
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008099:Liver / Q000378:metabolism*; D008297:Male; D017551:Microdialysis / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012717:Sesquiterpenes / Q000493:pharmacokinetics*; D045788:Sesquiterpenes, Germacrane / Q000493:pharmacokinetics*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D012717:Sesquiterpenes; D045788:Sesquiterpenes, Germacrane; C504126:curdione; C022801:curcumol
keywords: Curcumol; Curdione; Microdialysis; RRLC–MS; Rhizoma Curcumae