title: Synthesis, characterization and multi-spectroscopic DNA interaction studies of a new platinum complex containing the drug metformin.
abstract: A new platinum(II) complex; [Pt(Met)(DMSO)Cl]Cl in which Met = metformin and DMSO: dimethylsulfoxide, was synthesized and characterized by (1)H NMR, IR, UV-Vis spectra, molar conductivity and computational methods. Binding interaction of this complex with calf thymus (CT) DNA has been investigated by using absorption, emission, circular dichroism, viscosity measurements, differential pulse voltammetry and cleavage studies by agarose gel electrophoresis. UV-Vis absorption studies showed hyperchromism. CD studies showed less perturbation on the base stacking and helicity bands in the CD spectrum of CT-DNA (B→C structural transition). In fluorimeteric studies, the Pt(II) complex can bind with DNA-NR complex and forms a new non-fluorescence adduct. The anodic peak current in the differential pulse voltammogram of the Pt(II) complex decreased gradually with the addition of DNA. Cleavage experiments showed that the Pt(II) complex does not induce any cleavage under the experimental setup. Finally all results indicated that Pt(II) complex interact with DNA via groove binding mode.
authors.affiliation: Department of Inorganic Chemistry, Faculty of Chemistry, Razi University, Kermanshah, Iran. Electronic address: nahidshahabadi@yahoo.com.
authors.forename: Nahid
authors.initials: N
authors.lastname: Shahabadi
authors.affiliation: Department of Inorganic Chemistry, Faculty of Chemistry, Razi University, Kermanshah, Iran.
authors.forename: Leila
authors.initials: L
authors.lastname: Heidari
mesh_terms: D000818:Animals; D002417:Cattle; D004247:DNA / Q000737:chemistry*; D008687:Metformin / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D009944:Organoplatinum Compounds / Q000737:chemistry*; D010984:Platinum / Q000737:chemistry*
chemical_list: D009944:Organoplatinum Compounds; D010984:Platinum; D004247:DNA; D008687:Metformin
keywords: DNA; Groove binding mode; Metformin; Platinum complex; Spectroscopic methods