title: Chromosome mapping of repetitive sequences in four Serrasalmidae species (Characiformes).
abstract: The Serrasalmidae family is composed of a number of commercially interesting species, mainly in the Amazon region where most of these fishes occur. In the present study, we investigated the genomic organization of the 18S and 5S rDNA and telomeric sequences in mitotic chromosomes of four species from the basal clade of the Serrasalmidae family: Colossoma macropomum, Mylossoma aureum, M. duriventre, and Piaractus mesopotamicus, in order to understand the chromosomal evolution in the family. All the species studied had diploid numbers 2n = 54 and exclusively biarmed chromosomes, but variations of the karyotypic formulas were observed. C-banding resulted in similar patterns among the analyzed species, with heterochromatic blocks mainly present in centromeric regions. The 18S rDNA mapping of C. macropomum and P. mesopotamicus revealed multiple sites of this gene; 5S rDNA sites were detected in two chromosome pairs in all species, although not all of them were homeologs. Hybridization with a telomeric probe revealed signals in the terminal portions of chromosomes in all the species and an interstitial signal was observed in one pair of C. macropomum.
authors.affiliation: Laboratório de Genética Animal, Instituto Nacional de Pesquisa da Amazônia, Manaus, AM, Brazil .
authors.forename: Leila Braga
authors.initials: LB
authors.lastname: Ribeiro
authors.affiliation: Laboratório de Evolução Aplicada, Instituto de Ciências Biológicas, Universidade Federal do Amazonas, Manaus, AM, Brazil .
authors.forename: Daniele Aparecida
authors.initials: DA
authors.lastname: Matoso
authors.affiliation: Laboratório de Genética Animal, Instituto Nacional de Pesquisa da Amazônia, Manaus, AM, Brazil .
authors.forename: Eliana
authors.initials: E
authors.lastname: Feldberg
keywords: 5S rDNA; tambaqui and pacu; telomeric sequences