title: Snail modulates the assembly of fibronectin via α5 integrin for myocardial migration in zebrafish embryos.
abstract: The Snail family member snail encodes a zinc finger-containing transcriptional factor that is involved in heart formation. Yet, little is known about how Snail regulates heart development. Here, we identified that one of the duplicated snail genes, snai1b, was expressed in the heart region of zebrafish embryos. Depletion of Snai1b function dramatically reduced expression of α5 integrin, disrupted Fibronectin layer in the heart region, especially at the midline, and prevented migration of cardiac precursors, resulting in defects in cardiac morphology and function in zebrafish embryos. Injection of α5β1 protein rescued the Fibronectin layer and then the myocardial precursor migration in snai1b knockdown embryos. The results provide the molecular mechanism how Snail controls the morphogenesis of heart during embryonic development.
authors.affiliation: 1] Laboratory of Stem Cell Biology, Center for Medical Stem Cell Biology, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, 610041, China [2].
authors.forename: Liangjun
authors.initials: L
authors.lastname: Qiao
authors.affiliation: 1] Laboratory of Stem Cell Biology, Center for Medical Stem Cell Biology, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, 610041, China [2].
authors.forename: Hongwei
authors.initials: H
authors.lastname: Gao
authors.affiliation: Laboratory of Stem Cell Biology, Center for Medical Stem Cell Biology, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, 610041, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Laboratory of Stem Cell Biology, Center for Medical Stem Cell Biology, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, 610041, China.
authors.forename: Lulu
authors.initials: L
authors.lastname: Jing
authors.affiliation: Laboratory of Stem Cell Biology, Center for Medical Stem Cell Biology, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, 610041, China.
authors.forename: Chun
authors.initials: C
authors.lastname: Xiao
authors.affiliation: Laboratory of Stem Cell Biology, Center for Medical Stem Cell Biology, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, 610041, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: Laboratory of Stem Cell Biology, Center for Medical Stem Cell Biology, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, 610041, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Luo
authors.affiliation: Laboratory of Stem Cell Biology, Center for Medical Stem Cell Biology, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, 610041, China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Laboratory of Stem Cell Biology, Center for Medical Stem Cell Biology, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, 610041, China.
authors.forename: Wentong
authors.initials: W
authors.lastname: Meng
authors.affiliation: Laboratory of Stem Cell Biology, Center for Medical Stem Cell Biology, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, 610041, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Xu
authors.affiliation: Laboratory of Stem Cell Biology, Center for Medical Stem Cell Biology, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, 610041, China.
authors.forename: Xianming
authors.initials: X
authors.lastname: Mo
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000235:genetics; D004625:Embryo, Nonmammalian; D047108:Embryonic Development; D005353:Fibronectins / Q000235:genetics / Q000378:metabolism*; D006321:Heart / Q000254:growth & development; D039482:Integrin alpha5 / Q000235:genetics / Q000378:metabolism*; D009024:Morphogenesis / Q000235:genetics; D009206:Myocardium / Q000166:cytology; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors / Q000037:antagonists & inhibitors / Q000235:genetics*; D015027:Zebrafish; D029961:Zebrafish Proteins / Q000037:antagonists & inhibitors / Q000235:genetics*
chemical_list: D005353:Fibronectins; D039482:Integrin alpha5; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors; D029961:Zebrafish Proteins; C096850:snai1b protein, zebrafish