title: Surface modification of titanium substrates with silver nanoparticles embedded sulfhydrylated chitosan/gelatin polyelectrolyte multilayer films for antibacterial application.
abstract: To develop Ti implants with potent antibacterial activity, a novel "sandwich-type" structure of sulfhydrylated chitosan (Chi-SH)/gelatin (Gel) polyelectrolyte multilayer films embedding silver (Ag) nanoparticles was coated onto titanium substrate using a spin-assisted layer-by-layer assembly technique. Ag ions would be enriched in the polyelectrolyte multilayer films via the specific interactions between Ag ions and -HS groups in Chi-HS, thus leading to the formation of Ag nanoparticles in situ by photo-catalytic reaction (ultraviolet irradiation). Contact angle measurement and field emission scanning electron microscopy equipped with energy dispersive X-ray spectroscopy were employed to monitor the construction of Ag-containing multilayer on titanium surface, respectively. The functional multilayered films on titanium substrate [Ti/PEI/(Gel/Chi-SH/Ag) n /Gel] could efficiently inhibit the growth and activity of Bacillus subtitles and Escherichia coli onto titanium surface. Moreover, studies in vitro confirmed that Ti substrates coating with functional multilayer films remained the biological functions of osteoblasts, which was reflected by cell morphology, cell viability and ALP activity measurements. This study provides a simple, versatile and generalized methodology to design functional titanium implants with good cyto-compatibility and antibacterial activity for potential clinical applications.
authors.affiliation: Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, College of Bioengineering, Chongqing University, Chongqing, 400044, People's Republic of China.
authors.forename: Wen
authors.initials: W
authors.lastname: Li
authors.forename: Dawei
authors.initials: D
authors.lastname: Xu
authors.forename: Yan
authors.initials: Y
authors.lastname: Hu
authors.forename: Kaiyong
authors.initials: K
authors.lastname: Cai
authors.forename: Yingcheng
authors.initials: Y
authors.lastname: Lin
mesh_terms: D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000138:chemical synthesis; D018407:Bacterial Physiological Phenomena / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis; D004573:Electrolytes / Q000737:chemistry; D005780:Gelatin / Q000737:chemistry*; D008422:Materials Testing; D053768:Metal Nanoparticles / Q000008:administration & dosage / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D019736:Prostheses and Implants / Q000382:microbiology; D012834:Silver / Q000737:chemistry* / Q000494:pharmacology*; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D020099:Coated Materials, Biocompatible; D004573:Electrolytes; D012834:Silver; D005780:Gelatin; D048271:Chitosan; D014025:Titanium