title: Immunochemical determination of fluoroquinolone antibiotics in cattle hair: a strategy to ensure food safety.
abstract: Enrofloxacin (ERFX) is a synthetic antibiotic of the fluoroquinolone (FQ) family, which is commonly administered in veterinary medicine. ERFX and its metabolite, ciprofloxacin (CPFX), have been reported to accumulate in hair of treated animals. Therefore, hair analysis is an attractive non-invasive alternative to control misuse of such antibiotic and to ensure food safety by preventing such food derived products arrive to the consumer. In this context, an immunochemical analytical protocol has been established to detect ERFX and CPFX residues in cattle hair samples. Unpigmented and pigmented hair were collected from ERFX-treated and non-treated calves, and the aqueous NH4OH extracts were directly analyzed by ELISA, being possible to achieve limits of detection in the range of 10-30 μg kg(-1). A good concordance between HPLC and ELISA measurements was observed. The results demonstrate the potential of the immunochemical procedure reported here to rapidly screen and quantitate FQ residues in hair samples.
authors.affiliation: Nanobiotechnology for Diagnostics group (Nb4D), IQAC-CSIC, Jorge Girona, 18-26, 08034 Barcelona, Spain; CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Jorge Girona, 18-26, 08034 Barcelona, Spain.
authors.forename: Fátima
authors.initials: F
authors.lastname: Fernández
authors.affiliation: Nanobiotechnology for Diagnostics group (Nb4D), IQAC-CSIC, Jorge Girona, 18-26, 08034 Barcelona, Spain; CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Jorge Girona, 18-26, 08034 Barcelona, Spain.
authors.forename: Daniel G
authors.initials: DG
authors.lastname: Pinacho
authors.affiliation: Institute for Food and Agricultural Research and Technology, Meat Technology, Centre (IRTA-CTC) Food Chemistry Unit, Granja Camps i Armet s/n, Monells, 17121 Girona, Spain.
authors.forename: Marta
authors.initials: M
authors.lastname: Gratacós-Cubarsí
authors.affiliation: Institute for Food and Agricultural Research and Technology, Meat Technology, Centre (IRTA-CTC) Food Chemistry Unit, Granja Camps i Armet s/n, Monells, 17121 Girona, Spain.
authors.forename: José-Antonio
authors.initials: JA
authors.lastname: García-Regueiro
authors.affiliation: Institute for Food and Agricultural Research and Technology, Meat Technology, Centre (IRTA-CTC) Food Chemistry Unit, Granja Camps i Armet s/n, Monells, 17121 Girona, Spain.
authors.forename: Massimo
authors.initials: M
authors.lastname: Castellari
authors.affiliation: Nanobiotechnology for Diagnostics group (Nb4D), IQAC-CSIC, Jorge Girona, 18-26, 08034 Barcelona, Spain; CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Jorge Girona, 18-26, 08034 Barcelona, Spain.
authors.forename: Francisco
authors.initials: F
authors.lastname: Sánchez-Baeza
authors.affiliation: Nanobiotechnology for Diagnostics group (Nb4D), IQAC-CSIC, Jorge Girona, 18-26, 08034 Barcelona, Spain; CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Jorge Girona, 18-26, 08034 Barcelona, Spain. Electronic address: pilar.marco@cid.csic.es.
authors.forename: M-Pilar
authors.initials: MP
authors.lastname: Marco
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry*; D002417:Cattle; D024841:Fluoroquinolones / Q000737:chemistry*; D059022:Food Safety; D006197:Hair / Q000737:chemistry*; D007120:Immunochemistry
chemical_list: D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones
keywords: Antibiotics; ELISA; Fluoroquinolones; Food safety; HPLC; Hair analysis; Immunoassay