title: Magnetically responsive microflaps reveal cell membrane boundaries from multiple angles.
abstract: A microflap system to incline adherent cells in the desired orientation is described. Inclination angles of cell-laden microflaps are precisely controlled by the applied magnetic field, enabling us to observe cell-membrane boundaries from multiple angles. This system is equipped with conventional microscopes, allowing clear focused images of cell-membrane boundaries to be obtained with high magnification.
authors.affiliation: Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, 153-8505, Japan.
authors.forename: Tetsuhiko
authors.initials: T
authors.lastname: Teshima
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Onoe
authors.forename: Hiroka
authors.initials: H
authors.lastname: Aonuma
authors.forename: Kaori
authors.initials: K
authors.lastname: Kuribayashi-Shigetomi
authors.forename: Koki
authors.initials: K
authors.lastname: Kamiya
authors.forename: Taishi
authors.initials: T
authors.lastname: Tonooka
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Kanuka
authors.forename: Shoji
authors.initials: S
authors.lastname: Takeuchi
mesh_terms: D002462:Cell Membrane / Q000648:ultrastructure*; D016764:Cell Polarity; D002478:Cells, Cultured; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005347:Fibroblasts / Q000166:cytology*; D006801:Humans; D007089:Image Enhancement / Q000295:instrumentation*; D008280:Magnetics / Q000295:instrumentation*; D008846:Micromanipulation / Q000295:instrumentation* / Q000379:methods; D008853:Microscopy / Q000295:instrumentation*; D008904:Miniaturization
keywords: magnetic actuation; multi-angle observation; parasite infection