title: Crucial role of Notch signaling in osteogenic differentiation of periodontal ligament stem cells in osteoporotic rats.
abstract: Estrogen deficiency-induced osteoporosis typically occurs in postmenopausal women and has been strongly associated with periodontal diseases. Periodontal ligament stem cells (PDLSCs) isolated from the periodontal ligament can differentiate into many types of specialized cells, including osteoblast-like cells that contribute to periodontal tissue repair. The Notch signaling pathway is highly conserved and associated with self-renewal potential and cell-fate determination. Recently, several studies have focused on the relationship between Notch signaling and osteogenic differentiation. However, the precise mechanisms underlying this relationship are largely unknown. We have successfully isolated PDLSCs from both ovariectomized (OVX) and sham-operated rats. Both the mRNA and protein levels of Notch1 and Jagged1 were upregulated when PDLSCs were cultured in osteogenic induction media. Mineralization assays showed decreased calcium deposits in OVX-PDLSCs treated with a γ-secretase inhibitor compared with control cells. Thus Notch signaling is important in maintaining the osteogenic differentiation of PDLSCs in osteoporotic rats, which help in the development of a potential therapeutic strategy for periodontal disease in postmenopausal women.
authors.affiliation: Department of Stomatology, General Hospital of the Air Force of Chinese PLA, Beijing, 100853, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.forename: S Q
authors.initials: SQ
authors.lastname: Li
authors.forename: Y M
authors.initials: YM
authors.lastname: Gao
authors.forename: Jin
authors.initials: J
authors.lastname: Li
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
mesh_terms: D053829:Amyloid Precursor Protein Secretases / Q000037:antagonists & inhibitors; D000818:Animals; D002118:Calcium / Q000502:physiology; D002135:Calcium-Binding Proteins / Q000235:genetics; D005260:Female; D005269:Femur / Q000166:cytology; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics; D000072100:Jagged-1 Protein; D008565:Membrane Proteins / Q000235:genetics; D008963:Molar / Q000166:cytology; D010012:Osteogenesis / Q000502:physiology*; D010024:Osteoporosis / Q000473:pathology*; D010052:Ovariectomy; D010513:Periodontal Ligament / Q000166:cytology*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D051881:Receptor, Notch1 / Q000235:genetics / Q000378:metabolism*; D000072099:Serrate-Jagged Proteins; D015398:Signal Transduction; D013234:Stem Cells / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D002135:Calcium-Binding Proteins; D036341:Intercellular Signaling Peptides and Proteins; C000606275:Jag1 protein, rat; D000072100:Jagged-1 Protein; D008565:Membrane Proteins; C497763:Notch1 protein, rat; D012333:RNA, Messenger; D051881:Receptor, Notch1; D000072099:Serrate-Jagged Proteins; D053829:Amyloid Precursor Protein Secretases; D002118:Calcium
keywords: Notch signaling; osteogenic differentiation; osteoporosis; periodontal ligament stem cells