title: Percutaneous bone marrow transplantation using fractional ablative Erbium:YAG laser.
abstract: Topical application of therapeutic agents has been a mainstay in Dermatology for the treatment of skin disorders but is not commonly used for systemic delivery. For a topically applied agent to reach distant body sites it must first overcome the barrier function of the skin and then penetrate into deeper structures before reaching the systemic circulation. This has limited the use of topically applied agents to those having specific charge, solubility and size restrictions. Pretreatment of the skin with ablative fractional laser appears to enhance the uptake of some topically applied drugs but the ability to effectively deliver agents to distant sites is largely unproven. In this report we used a fractional ablative Erb:YAG (Erbium/Yttrium Aluminum Garnet) laser to facilitate the transfer of bone marrow stem cells through the skin in a murine bone marrow transplant model. Chimerism could be detected in the peripheral blood of recipient C57BL/6 mice that were pretreated with ablative fractional laser and had topically applied enhanced green fluorescent protein (GFP) labeled bone marrow cells from syngeneic donor transgenic mice. This study indicates that fractional laser can be used to deliver stem cells through the skin and remain functionally intact.
authors.affiliation: Department of Dermatology and Cutaneous Surgery, University of Miami Miller School of Medicine, Miami, Florida, United States of America; Interdiscipinary Stem Cell Institute, University of Miami Miller School of Medicine, Miami, Florida, United States of America.
authors.forename: Luis
authors.initials: L
authors.lastname: Rodriguez-Menocal
authors.affiliation: Department of Dermatology and Cutaneous Surgery, University of Miami Miller School of Medicine, Miami, Florida, United States of America; Interdiscipinary Stem Cell Institute, University of Miami Miller School of Medicine, Miami, Florida, United States of America.
authors.forename: Marcela
authors.initials: M
authors.lastname: Salgado
authors.affiliation: Department of Dermatology and Cutaneous Surgery, University of Miami Miller School of Medicine, Miami, Florida, United States of America.
authors.forename: Stephen
authors.initials: S
authors.lastname: Davis
authors.affiliation: Department of Dermatology and Cutaneous Surgery, University of Miami Miller School of Medicine, Miami, Florida, United States of America.
authors.forename: Jill
authors.initials: J
authors.lastname: Waibel
authors.affiliation: Department of Dermatology and Cutaneous Surgery, University of Miami Miller School of Medicine, Miami, Florida, United States of America.
authors.forename: Arsalan
authors.initials: A
authors.lastname: Shabbir
authors.affiliation: Department of Dermatology and Cutaneous Surgery, University of Miami Miller School of Medicine, Miami, Florida, United States of America.
authors.forename: Audrey
authors.initials: A
authors.lastname: Cox
authors.affiliation: Department of Dermatology and Cutaneous Surgery, University of Miami Miller School of Medicine, Miami, Florida, United States of America; Interdiscipinary Stem Cell Institute, University of Miami Miller School of Medicine, Miami, Florida, United States of America.
authors.forename: Evangelos V
authors.initials: EV
authors.lastname: Badiavas
mesh_terms: D055011:Ablation Techniques / Q000379:methods*; D000818:Animals; D016026:Bone Marrow Transplantation / Q000379:methods*; D005260:Female; D049452:Green Fluorescent Proteins / Q000235:genetics; D053844:Lasers, Solid-State; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D012867:Skin
chemical_list: C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins