title: Detection of donor-specific-antibodies by solid phase assay and its relevance to complement-dependent-lymphocytotoxicity cross-matching in kidney transplantation.
abstract: Presensitization against a broad array of HLA is associated with prolonged waiting times and inferior kidney allogaft survival. Although the use of solid phase assay (SPA) for the detection and characterization of anti-HLA antibodies provides greater sensitivity than complement-dependent lymphocytotoxicity (CDC) assay, it often detects donor specific antibodies (DSA) which turn out to be clinically irrelevant. Our data reinforce the concept that these two types of assays should be used in parallel for pre-and post-transplantation monitoring of anti-HLA antibodies in recipients of solid organ allografts.
authors.affiliation: Department of Pathology & Cell Biology, Columbia University, New York, NY 10032, USA.
authors.forename: Eric K
authors.initials: EK
authors.lastname: Ho
authors.affiliation: Department of Pathology & Cell Biology, Columbia University, New York, NY 10032, USA.
authors.forename: Elena R
authors.initials: ER
authors.lastname: Vasilescu
authors.affiliation: Department of Pathology & Cell Biology, Columbia University, New York, NY 10032, USA.
authors.forename: George
authors.initials: G
authors.lastname: Vlad
authors.affiliation: Department of Pathology & Cell Biology, Columbia University, New York, NY 10032, USA.
authors.forename: Raphael A
authors.initials: RA
authors.lastname: Clynes
authors.affiliation: Department of Surgery, Columbia University, New York, NY 10032, USA.
authors.forename: Lloyd E
authors.initials: LE
authors.lastname: Ratner
authors.affiliation: Department of Pathology & Cell Biology, Columbia University, New York, NY 10032, USA. Electronic address: ns20@columbia.edu.
authors.forename: Nicole
authors.initials: N
authors.lastname: Suciu-Foca
mesh_terms: D000328:Adult; D000368:Aged; D000961:Antilymphocyte Serum / Q000097:blood*; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology; D003165:Complement System Proteins / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D006084:Graft Rejection / Q000517:prevention & control; D006085:Graft Survival; D006680:HLA Antigens / Q000235:genetics / Q000276:immunology; D006650:Histocompatibility Testing; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007518:Isoantibodies / Q000097:blood*; D016030:Kidney Transplantation; D008297:Male; D008875:Middle Aged; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology; D014019:Tissue Donors; D014184:Transplantation, Homologous
chemical_list: D000961:Antilymphocyte Serum; D006680:HLA Antigens; D007518:Isoantibodies; D003165:Complement System Proteins
keywords: Complement-dependent lymphocytotoxicity; Cross-matching; Donor-specific antibodies; Kidney transplantation; Solid phase assay