title: Comparison of extraction methods for analysis of citrinin in red fermented rice.
abstract: There is no standard method for the extraction and analysis of citrinin in red fermented rice (RFR). In the study, five extraction methods were compared for their efficiency to analyse citrinin in RFR by HPLC-FLD (reversed phase high performance liquid chromatography with fluorescence detection), including, (1) ultrasonic extraction with EW solution (ethanol:water, 7:3, v/v); (2) ultrasonic extraction with TEF solvent mixtures (toluene:ethyl acetate:formic acid, 7:3:1, v/v); (3) shaking extraction with EW; (4) shaking extraction with EF solvent mixtures (ethyl acetate:formic acid, 1:1, v/v); (5) shaking combined with ultrasonic extraction in EW. Comparison of chromatograms of citrinin by HPLC-FLD with different extraction methods revealed that EW was the best extraction solvent. It was also found that shaking combined with ultrasonic extraction in EW was the most efficient extraction method to extract citrinin from RFR for qualitative and quantitative analysis.
authors.affiliation: Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Collaborative Innovation Center for Industrial Fermentation, Hubei University of Technology, Wuhan 430068, China. Electronic address: Wang1wei1ping1@163.com.
authors.forename: Weiping
authors.initials: W
authors.lastname: Wang
authors.affiliation: Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Collaborative Innovation Center for Industrial Fermentation, Hubei University of Technology, Wuhan 430068, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Collaborative Innovation Center for Industrial Fermentation, Hubei University of Technology, Wuhan 430068, China.
authors.forename: Xinfeng
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Collaborative Innovation Center for Industrial Fermentation, Hubei University of Technology, Wuhan 430068, China.
authors.forename: Huashan
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Collaborative Innovation Center for Industrial Fermentation, Hubei University of Technology, Wuhan 430068, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Huang
authors.affiliation: Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Collaborative Innovation Center for Industrial Fermentation, Hubei University of Technology, Wuhan 430068, China. Electronic address: Dongshengli86@163.com.
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Li
authors.affiliation: Wuhan Jiacheng Biotechnology Co., Ltd., Wuhan 430074, China.
authors.forename: Jicheng
authors.initials: J
authors.lastname: Yao
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002953:Citrinin / Q000009:adverse effects* / Q000032:analysis; D005285:Fermentation; D012275:Oryza / Q000737:chemistry*
chemical_list: D002953:Citrinin
keywords: Citrinin; Extraction methods; Fed fermented rice; HPLC–FLD analysis