title: [The role of Foxo1 and ubiquitin-proteasome system markers in muscle atrophy caused by chronic kidney disease].
abstract: OBJECTIVE
  To identity whether there is muscle atrophy phenomenon in end-stage kidney disease patients and to detect the level of transcription factor Foxo1 and the activity of ubiquitin-proteasome system.
  
  
  METHODS
  Twenty-two patients in chronic kidney disease (CKD) stage 5 were selected and their mean muscle cross sectional area was measured. mRNA and protein levels of Foxo1, Atrogin-1, MuRF1 in rectus abdominis biopsies obtained from consecutive patients were detected. Control biopsies were obtained from 8 healthy subjects during elective surgery for abdominal wall hernias and 6 subjects during elective surgery for adenomyosis.
  
  
  RESULTS
  Compared with the control group, cross sectional area of muscle fibers decreased and the transcription and protein levels of Foxo1, Atrogin-1, MuRF1 were upregulated in CKD group (P < 0.05). Protein level of p-Foxo1 decreased in CKD group (P < 0.05).
  
  
  CONCLUSIONS
  There exist muscle atrophy phenomenon in CKD patients, which may associate with the upregulation of Foxo1 and activation of ubiquitin-proteasome system.
authors.forename: Jun
authors.initials: J
authors.lastname: Yin
authors.forename: Juan
authors.initials: J
authors.lastname: Huang
authors.affiliation: Department of Nephrology, the First Affiliated People's Hospital, Shanghai Jiaotong University, Shanghai 200080, China. Email: ywj4169@163.com.
authors.forename: Weijie
authors.initials: W
authors.lastname: Yuan
authors.forename: Lijie
authors.initials: L
authors.lastname: Gu
authors.forename: Ling
authors.initials: L
authors.lastname: Wang
authors.forename: Minghua
authors.initials: M
authors.lastname: Shang
mesh_terms: D016022:Case-Control Studies; D005260:Female; D000071161:Forkhead Box Protein O1; D051858:Forkhead Transcription Factors / Q000378:metabolism*; D006801:Humans; D007676:Kidney Failure, Chronic / Q000150:complications* / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009124:Muscle Proteins / Q000378:metabolism; D009133:Muscular Atrophy / Q000209:etiology*; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism*; D017568:Rectus Abdominis / Q000378:metabolism; D044843:SKP Cullin F-Box Protein Ligases / Q000378:metabolism; D000070584:Tripartite Motif Proteins; D025801:Ubiquitin / Q000378:metabolism; D044767:Ubiquitin-Protein Ligases / Q000378:metabolism
chemical_list: C085126:FOXO1 protein, human; D000071161:Forkhead Box Protein O1; D051858:Forkhead Transcription Factors; D009124:Muscle Proteins; D000070584:Tripartite Motif Proteins; D025801:Ubiquitin; C440907:FBXO32 protein, human; D044843:SKP Cullin F-Box Protein Ligases; C440908:TRIM63 protein, human; D044767:Ubiquitin-Protein Ligases; D046988:Proteasome Endopeptidase Complex