title: Preparation and biological activity of a paclitaxel-single-walled carbon nanotube complex.
abstract: Single-walled carbon nanotubes (SWCNTs) have unique transmembrane abilities. The huge superficial area and abundance of π electrons confer SWCNTs perfect absorptive capability toward proteins, nucleates, and many drugs. These characteristics make SWCNTs a new and efficient drug carrier. The purpose of this study was to disperse SWCNTs in water and have paclitaxel absorbed onto them in order to construct an asparagine-glycine-arginine (NGR)-SWCNT-Paclitaxel complex as a targeting nanoparticle system. The NGR-SWCNT-Paclitaxel complex was systematically studied, and analytical methods, including spectrophotometry for SWCNTs and high-performance liquid chromatography for paclitaxel, were employed. The preparation and the prescription of the NGR-SWCNT-Paclitaxel complex lyophilized powder were investigated. MCF-7 cancer cells, Sprague-Dawley rats, and S180 tumor-bearing mice were used as experimental subjects to evaluate the in vitro and in vivo activity of NGR-SWCNT-Paclitaxel complex dispersion. The complex dispersion showed obvious inhibition activity against MCF-7 cancer cells. Within 1 h, the NGR-SWCNT-Paclitaxel complex could be transferred to cells, and sustained the release of drugs. In addition, the tumor and liver targeting and improved therapeutic effects of the NGR-SWCNT-Paclitaxel complex were confirmed.
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
authors.forename: X D
authors.initials: XD
authors.lastname: Fu
authors.affiliation: Basic Medical Sciences College of Zhengzhou University, Zhengzhou, China.
authors.forename: Y Y
authors.initials: YY
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery, The Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
authors.forename: X J
authors.initials: XJ
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, The Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
authors.forename: J X
authors.initials: JX
authors.lastname: Shou
authors.affiliation: School of Pharmacy of Zhengzhou University, Zhengzhou, China.
authors.forename: Z Z
authors.initials: ZZ
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China xddoccn@126.com.
authors.forename: L J
authors.initials: LJ
authors.lastname: Song
mesh_terms: D000818:Animals; D016503:Drug Delivery Systems; D006801:Humans; D051379:Mice; D037742:Nanotubes, Carbon / Q000737:chemistry*; D017239:Paclitaxel / Q000008:administration & dosage* / Q000737:chemistry; D051381:Rats; D014867:Water
chemical_list: D037742:Nanotubes, Carbon; D014867:Water; D017239:Paclitaxel