title: Transcriptome analysis of an mvp mutant reveals important changes in global gene expression and a role for methyl jasmonate in vernalization and flowering in wheat.
abstract: The einkorn wheat mutant mvp-1 (maintained vegetative phase 1) has a non-flowering phenotype caused by deletions including, but not limited to, the genes CYS, PHYC, and VRN1. However, the impact of these deletions on global gene expression is still unknown. Transcriptome analysis showed that these deletions caused the upregulation of several pathogenesis-related (PR) and jasmonate-responsive genes. These results suggest that jasmonates may be involved in flowering and vernalization in wheat. To test this hypothesis, jasmonic acid (JA) and methyl jasmonate (MeJA) content in mvp and wild-type plants was measured. The content of JA was comparable in all plants, whereas the content of MeJA was higher by more than 6-fold in mvp plants. The accumulation of MeJA was also observed in vernalization-sensitive hexaploid winter wheat during cold exposure. This accumulation declined rapidly once plants were deacclimated under floral-inductive growth conditions. This suggests that MeJA may have a role in floral transition. To confirm this result, we treated vernalization-insensitive spring wheat with MeJA. The treatment delayed flowering with significant downregulation of both TaVRN1 and TaFT1 genes. These data suggest a role for MeJA in modulating vernalization and flowering time in wheat.
authors.affiliation: Département des Sciences biologiques Université du Québec à Montréal, CP 8888, Succursale Centre-Ville Montréal, Québec H3C 3P8 Canada.
authors.forename: Amadou Oury
authors.initials: AO
authors.lastname: Diallo
authors.affiliation: Département des Sciences biologiques Université du Québec à Montréal, CP 8888, Succursale Centre-Ville Montréal, Québec H3C 3P8 Canada.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Agharbaoui
authors.affiliation: Département des Sciences biologiques Université du Québec à Montréal, CP 8888, Succursale Centre-Ville Montréal, Québec H3C 3P8 Canada.
authors.forename: Mohamed A
authors.initials: MA
authors.lastname: Badawi
authors.affiliation: Département des Sciences biologiques Université du Québec à Montréal, CP 8888, Succursale Centre-Ville Montréal, Québec H3C 3P8 Canada.
authors.forename: Mohamed Ali
authors.initials: MA
authors.lastname: Ali-Benali
authors.affiliation: Département des Sciences biologiques Université du Québec à Montréal, CP 8888, Succursale Centre-Ville Montréal, Québec H3C 3P8 Canada.
authors.forename: Amira
authors.initials: A
authors.lastname: Moheb
authors.affiliation: Département des Sciences biologiques Université du Québec à Montréal, CP 8888, Succursale Centre-Ville Montréal, Québec H3C 3P8 Canada.
authors.forename: Mario
authors.initials: M
authors.lastname: Houde
authors.affiliation: Département des Sciences biologiques Université du Québec à Montréal, CP 8888, Succursale Centre-Ville Montréal, Québec H3C 3P8 Canada sarhan.fathey@uqam.ca.
authors.forename: Fathey
authors.initials: F
authors.lastname: Sarhan
mesh_terms: D000085:Acetates / Q000378:metabolism*; D003080:Cold Temperature; D003517:Cyclopentanes / Q000378:metabolism*; D035264:Flowers / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D054883:Oxylipins / Q000378:metabolism*; D010937:Plant Growth Regulators / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D012621:Seasons; D014158:Transcription, Genetic; D014908:Triticum / Q000235:genetics* / Q000378:metabolism
chemical_list: D000085:Acetates; D003517:Cyclopentanes; D054883:Oxylipins; D010937:Plant Growth Regulators; D010940:Plant Proteins; C072239:methyl jasmonate
keywords: Biotic stress; flowering; maintained vegetative phase; methyl jasmonate; vernalization; wheat.