title: The proteomics quantification dilemma.
abstract: Proteomics is dominated today by the protein expression discourse, which favorites the bottom-up approach because of its high throughput and its high sensitivity. For quantification this proceeding is misleading, if a protein is present with more than one protein species in the sample to be analyzed. The protein speciation discourse considers this more realistic situation and affords the top-down procedures or at least a separation of the protein species in advance to identification and quantification. Today all of the top-down procedures are one order of magnitude less sensitive than the bottom-up ones. To increase sensitivity and to increase throughput are major challenges for proteomics of the next years. This article is part of a Special Issue entitled: 20years of Proteomics in memory of Viatliano Pallini. Guest Editors: Luca Bini, Juan J. Calvete, Natacha Turck, Denis Hochstrasser and Jean-Charles Sanchez.
authors.affiliation: Max Planck Institute for Infection Biology, Core Facility Protein Analysis, Berlin, Germany.
authors.forename: Peter R
authors.initials: PR
authors.lastname: Jungblut
mesh_terms: D000846:Anniversaries and Special Events; D010506:Periodicals as Topic; D011506:Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D040901:Proteomics / Q000379:methods*
chemical_list: D011506:Proteins
keywords: Bottom-up proteomics; Protein species; Proteoform; Quantification; Top-down proteomics