title: Activated androgen receptor promotes bladder cancer metastasis via Slug mediated epithelial-mesenchymal transition.
abstract: Androgen receptor (AR) has been indicated to be involved in bladder cancer progression. We showed androgen induced epithelial-mesenchymal transition (EMT) in AR-positive bladder cancer cells and promoted tumor metastasis in xenograft models. We subsequently identified that Slug was the mediator of EMT induced by androgen. Furthermore, upregulation of Slug was due to activation of Wnt/β-catenin signaling in response to androgen. Finally, expression of AR showed strong correlation with loss of E-cadherin, higher expression of Slug and nuclear accumulation of β-catenin in bladder tumor tissues. Taken together, our results suggest AR signaling promotes bladder cancer metastasis through Slug mediated EMT.
authors.affiliation: Department of Urology, The Affiliated First People's Hospital of Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Yifeng
authors.initials: Y
authors.lastname: Jing
authors.affiliation: Department of Urology, The Affiliated First People's Hospital of Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Di
authors.initials: D
authors.lastname: Cui
authors.affiliation: Department of Pathology, Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education, Institutes of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Wenhuan
authors.initials: W
authors.lastname: Guo
authors.affiliation: Department of Urology, The Affiliated First People's Hospital of Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Juntao
authors.initials: J
authors.lastname: Jiang
authors.affiliation: Department of Urology, The Affiliated First People's Hospital of Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Jiang
authors.affiliation: Department of Urology, The Affiliated First People's Hospital of Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Youyi
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Department of Urology, The Affiliated First People's Hospital of Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Department of Urology, The Affiliated First People's Hospital of Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Xiaohai
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Urology, The Affiliated First People's Hospital of Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Jiang
authors.affiliation: Department of Urology, The Affiliated First People's Hospital of Shanghai Jiao Tong University School of Medicine, Shanghai, China. Electronic address: hanbm@163.com.
authors.forename: Bangmin
authors.initials: B
authors.lastname: Han
authors.affiliation: Department of Urology, The Affiliated First People's Hospital of Shanghai Jiao Tong University School of Medicine, Shanghai, China. Electronic address: xsjurologist@163.com.
authors.forename: Shujie
authors.initials: S
authors.lastname: Xia
mesh_terms: D021581:Active Transport, Cell Nucleus; D000818:Animals; D015703:Antigens, CD; D015820:Cadherins / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement; D058750:Epithelial-Mesenchymal Transition; D015972:Gene Expression Regulation, Neoplastic; D064593:Heterografts; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009361:Neoplasm Invasiveness; D034622:RNA Interference; D011944:Receptors, Androgen / Q000235:genetics / Q000378:metabolism*; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014162:Transfection; D001749:Urinary Bladder Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D060449:Wnt Signaling Pathway; D051176:beta Catenin / Q000378:metabolism
chemical_list: C410798:AR protein, human; D015703:Antigens, CD; C513384:CDH1 protein, human; C495270:CTNNB1 protein, human; D015820:Cadherins; D011944:Receptors, Androgen; C000604407:SNAI1 protein, human; C000604449:Snai2 protein, mouse; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors; D051176:beta Catenin
keywords: Androgen receptor; Bladder cancer; Epithelial-mesenchymal transition; Slug