title: Maiden outbreaks of dengue virus 1 genotype III in rural central India.
abstract: Dengue is regarded as the most important arboviral disease. Although sporadic cases have been reported, serotypes responsible for outbreaks have not been identified from central India over the last 20 years. We investigated two outbreaks of febrile illness, in August and November 2012, from Korea district (Chhattisgarh) and Narsinghpur district (Madhya Pradesh), respectively. Fever and entomological surveys were conducted in the affected regions. Molecular and serological tests were conducted on collected serum samples. Dengue-specific amplicons were sequenced and phylogenetic analyses were performed. In Korea and Narsinghpur districts 37·3% and 59% of cases were positive, respectively, for dengue infection, with adults being the worst affected. RT-PCR confirmed dengue virus serotype 1 genotype III as the aetiology. Ninety-six percent of infections were primary. This is the first time that dengue virus 1 outbreaks have been documented from central India. Introduction of the virus into the population and a conducive mosquitogenic environment favouring increased vector density caused the outbreak. Timely diagnosis and strengthening vector control measures are essential to avoid future outbreaks.
authors.affiliation: Virology Diagnostic Laboratory,Regional Medical Research Centre for Tribals (ICMR),Jabalpur,India.
authors.forename: P V
authors.initials: PV
authors.lastname: Barde
authors.affiliation: Virology Diagnostic Laboratory,Regional Medical Research Centre for Tribals (ICMR),Jabalpur,India.
authors.forename: B K
authors.initials: BK
authors.lastname: Kori
authors.affiliation: Virology Diagnostic Laboratory,Regional Medical Research Centre for Tribals (ICMR),Jabalpur,India.
authors.forename: M K
authors.initials: MK
authors.lastname: Shukla
authors.affiliation: Virology Diagnostic Laboratory,Regional Medical Research Centre for Tribals (ICMR),Jabalpur,India.
authors.forename: P K
authors.initials: PK
authors.lastname: Bharti
authors.affiliation: Virology Diagnostic Laboratory,Regional Medical Research Centre for Tribals (ICMR),Jabalpur,India.
authors.forename: G
authors.initials: G
authors.lastname: Chand
authors.affiliation: National Vector Borne Disease Control Programme, Chhattisgarh,India.
authors.forename: G
authors.initials: G
authors.lastname: Kumar
authors.affiliation: Virology Diagnostic Laboratory,Regional Medical Research Centre for Tribals (ICMR),Jabalpur,India.
authors.forename: M J
authors.initials: MJ
authors.lastname: Ukey
authors.affiliation: Virology Diagnostic Laboratory,Regional Medical Research Centre for Tribals (ICMR),Jabalpur,India.
authors.forename: N A
authors.initials: NA
authors.lastname: Ali
authors.affiliation: Virology Diagnostic Laboratory,Regional Medical Research Centre for Tribals (ICMR),Jabalpur,India.
authors.forename: N
authors.initials: N
authors.lastname: Singh
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D003715:Dengue / Q000453:epidemiology* / Q000821:virology*; D003716:Dengue Virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D004196:Disease Outbreaks / Q000706:statistics & numerical data*; D005260:Female; D005838:Genotype; D006801:Humans; D007194:India / Q000453:epidemiology; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008875:Middle Aged; D010802:Phylogeny; D012424:Rural Population; D055815:Young Adult