title: Detection of high-risk human papillomavirus type 16 and 18 using isothermal helicase-dependent amplification.
abstract: Persistent infection with human papillomavirus (HPV) induces cervical cancer. Here, we describe a sensitive, specific, and rapid assay for high-risk HPV16 and 18 detection by isothermal helicase-dependent amplification. This method can be used as cost-effective diagnostic method for low-income countries, where highest incidences worldwide of cervical cancer are registered.
authors.affiliation: Department of Haematology, Oncology and Laboratory Medicine, Unit of Microbiology, University Hospital S. Orsola-Malpighi, University of Bologna, via Massarenti 9, 40138 Bologna, Italy; Division of Viral Transformation Mechanisms, German Cancer Research Center, Im Neuenheimer Feld 242, D-69120 Heidelberg, Germany.
authors.forename: Daniela
authors.initials: D
authors.lastname: Barbieri
authors.affiliation: Department of Haematology, Oncology and Laboratory Medicine, Unit of Microbiology, University Hospital S. Orsola-Malpighi, University of Bologna, via Massarenti 9, 40138 Bologna, Italy.
authors.forename: Simona
authors.initials: S
authors.lastname: Venturoli
authors.affiliation: Division of Viral Transformation Mechanisms, German Cancer Research Center, Im Neuenheimer Feld 242, D-69120 Heidelberg, Germany.
authors.forename: Frank
authors.initials: F
authors.lastname: Rösl
authors.affiliation: Division of Viral Transformation Mechanisms, German Cancer Research Center, Im Neuenheimer Feld 242, D-69120 Heidelberg, Germany. Electronic address: b.orozco@dkfz-heidelberg.de.
authors.forename: Bladimiro
authors.initials: B
authors.lastname: Rincon-Orozco
mesh_terms: D005260:Female; D052162:Human papillomavirus 16 / Q000235:genetics / Q000302:isolation & purification*; D052161:Human papillomavirus 18 / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008403:Mass Screening / Q000379:methods; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D030361:Papillomavirus Infections / Q000175:diagnosis* / Q000821:virology*; D012680:Sensitivity and Specificity; D013696:Temperature
keywords: Cervical cancer; Cost-effective method; HPV detection; Isothermal helicase-dependent amplification