title: [Co-expression of BMP2 and Sox9 promotes chondrogenic differentiation of mesenchymal stem cells in vitro].
abstract: OBJECTIVE
  To investigate the effect of co-expression of bone morphogenetic protein 2 (BMP2) and Sox9 on chondrogenic differentiation of mesenchymal stem cells (MSCs) in vitro and provide experimental evidence for tissue engineering of cartilage.
  
  
  METHODS
  Mouse embryonic bone marrow MSC C3H10T1/2 cells were infected with recombinant adenovirus expressing BMP2, Sox9 and green fluorescent protein (GFP) for 3-14 days, with cells infected with the adenovirus carrying GFP gene as the control. The mRNA expression of the markers of chondrogenic differentiation, including collagen type II (Col2a1), aggrecan (ACAN), and collagen type X (Col10a1), were determined by real-time PCR. Alcian blue staining was used for quantitative analysis of sulfated glycosaminoglycan in the cellular matrix. The expression of Col2a1 protein was assayed by immunohistochemical staining and Western blot analysis.
  
  
  RESULTS
  Adenovirus-mediated BMP2 expression induced chondrogenic differentiation of C3H10T1/2 cells. Overexpression of Sox9 effectively enhanced BMP2-induced expression of the chondrogenic markers Col2a1, aggrecan and Col10a1 mRNAs, and promoted the synthesis of sulfated glycosaminoglycan and Col2a1 protein in C3H10T1/2 cells.
  
  
  CONCLUSIONS
  Co-expression of BMP2 and Sox9 can promote chondrogenic differentiation of MSCs in vitro, which provides a new strategy for tissue engineering of cartilage.
authors.affiliation: Department of Orthopedics, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China. E-mail: liaojunyi2011@126.com.
authors.forename: Junyi
authors.initials: J
authors.lastname: Liao
authors.forename: Nian
authors.initials: N
authors.lastname: Zhou
authors.forename: Liangbo
authors.initials: L
authors.lastname: Lin
authors.forename: Shixiong
authors.initials: S
authors.lastname: Yi
authors.forename: Tingxu
authors.initials: T
authors.lastname: Fan
authors.forename: Chen
authors.initials: C
authors.lastname: Zhao
authors.forename: Ning
authors.initials: N
authors.lastname: Hu
authors.forename: Xi
authors.initials: X
authors.lastname: Liang
authors.forename: Weike
authors.initials: W
authors.lastname: Si
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000235:genetics* / Q000378:metabolism; D002356:Cartilage / Q000166:cytology; D002454:Cell Differentiation; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D055758:SOX9 Transcription Factor / Q000235:genetics* / Q000378:metabolism; D023822:Tissue Engineering
chemical_list: D055396:Bone Morphogenetic Protein 2; D055758:SOX9 Transcription Factor