title: Effects of β-glucan polysaccharide revealed by the dominant lethal assay and micronucleus assays, and reproductive performance of male mice exposed to cyclophosphamide.
abstract: β-glucan is a well-known polysaccharide for its chemopreventive effect. This study aimed to evaluate the chemopreventive ability of β-glucan in somatic and germ cells through the dominant lethal and micronucleus assays, and its influence on the reproductive performance of male mice exposed to cyclophosphamide. The results indicate that β-glucan is capable of preventing changes in DNA in both germ cells and somatic ones. Changes in germ cells were evaluated by the dominant lethal assay and showed damage reduction percentages of 46.46% and 43.79% for the doses of 100 and 150 mg/kg. For the somatic changes, evaluated by micronucleus assay in peripheral blood cells in the first week of treatment, damage reduction percentages from 80.63-116.32% were found. In the fifth and sixth weeks, the percentage ranged from 10.20-52.54% and -0.95-62.35%, respectively. Besides the chemopreventive efficiency it appears that the β-glucan, when combined with cyclophosphamide, is able to improve the reproductive performance of males verified by the significant reduction in rates of post-implantation losses and reabsorption in the mating of nulliparous females with males treated with cyclophosphamide.
authors.affiliation: Centro de Estudos em Célula Tronco, Terapia Celular e Genética Toxicológica, Núcleo de Hospital Universitário, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil . ; Programa de Pós-Graduação em Saúde em Desenvolvimento na Região Centro-Oeste, Faculdade de Medicina "Dr. Hélio Mandetta", Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil . ; Programa de Mestrado em Farmácia, Centro de Ciências Biológicas e da Saúde, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil .
authors.forename: Rodrigo Juliano
authors.initials: RJ
authors.lastname: Oliveira
authors.affiliation: Centro de Estudos em Célula Tronco, Terapia Celular e Genética Toxicológica, Núcleo de Hospital Universitário, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil . ; Programa de Pós-Graduação em Saúde em Desenvolvimento na Região Centro-Oeste, Faculdade de Medicina "Dr. Hélio Mandetta", Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil .
authors.forename: João Renato
authors.initials: JR
authors.lastname: Pesarini
authors.affiliation: Departamento de Biologia Geral, Universidade Estadual de Londrina, Londrina, PR, Brazil .
authors.forename: Maria José
authors.initials: MJ
authors.lastname: Sparça Salles
authors.affiliation: Departamento de Biologia Geral, Universidade Estadual de Londrina, Londrina, PR, Brazil .
authors.forename: Tatiane Yumi
authors.initials: TY
authors.lastname: Nakamura Kanno
authors.affiliation: Departamento de Biologia Geral, Universidade Estadual de Londrina, Londrina, PR, Brazil .
authors.forename: Ana Carolina
authors.initials: AC
authors.lastname: Dos Santos Lourenço
authors.affiliation: Departamento de Biologia Geral, Universidade Estadual de Londrina, Londrina, PR, Brazil .
authors.forename: Véssia
authors.initials: V
authors.lastname: da Silva Leite
authors.affiliation: Departamento de Biologia Geral, Universidade Estadual de Londrina, Londrina, PR, Brazil .
authors.forename: Ariane Fernanda
authors.initials: AF
authors.lastname: da Silva
authors.affiliation: Laboratório de Tecnologia em Alimentos e Medicamentos, Universidade Estadual de Londrina, Londrina, PR, Brazil .
authors.forename: Hevenilton José
authors.initials: HJ
authors.lastname: Matiazi
authors.affiliation: Programa de Pós-Graduação em Biologia Celular e Molecular, Instituto de Biociências, Universidade Estadual Paulista "Júlio de Mesquita Filho", Rio Claro, SP, Brazil .
authors.forename: Lúcia Regina
authors.initials: LR
authors.lastname: Ribeiro
authors.affiliation: Departamento de Biologia Geral, Universidade Estadual de Londrina, Londrina, PR, Brazil .
authors.forename: Mário Sérgio
authors.initials: MS
authors.lastname: Mantovani
keywords: chemoprevention; micronucleus; mutation; nulliparous females; post-implantation losses