title: Gemella parahaemolysans sp. nov. and Gemella taiwanensis sp. nov., isolated from human clinical specimens.
abstract: Four Gram-staining-positive, catalase-negative, coccoid isolates, designated NTUH_1465(T), NTUH_2196, NTUH_4957 and NTUH_5572(T), were isolated from human specimens. The four isolates displayed more than 99.6% 16S rRNA gene sequence similarity with Gemella haemolysans ATCC 10379(T), and 96.7 to 98.6% similarity with Gemella sanguinis ATCC 700632(T), Gemella morbillorum ATCC 27824(T) or Gemella cuniculi CCUG 42726(T). However, phylogenetic analysis of concatenated sequences of three housekeeping genes, groEL, rpoB and recA, suggested that the four isolates were distinct from G. haemolysans ATCC 10379(T) and other species. Isolates NTUH_2196, NTUH_4957 and NTUH_5572(T) clustered together and formed a stable monophyletic clade. DNA-DNA hybridization values among strains NTUH_1465(T) and NTUH_5572(T) and their phylogenetically related neighbours were all lower than 49%. The four isolates could be distinguished from G. haemolysans and other species by phenotypic characteristics. Based on the phylogenetic and phenotypic results, two novel species Gemella parahaemolysans sp. nov. (type strain NTUH_1465(T) = BCRC 80365(T) = JCM 18067(T)) and Gemella taiwanensis sp. nov. (type strain NTUH_5572(T) = BCRC 80366(T) = JCM 18066(T)) are proposed.
authors.affiliation: Department of Clinical Laboratory Sciences and Medical Biotechnology, National Taiwan University College of Medicine, Taipei, Taiwan, ROC.
authors.forename: Wei-Chun
authors.initials: WC
authors.lastname: Hung
authors.affiliation: Department of Clinical Laboratory Sciences and Medical Biotechnology, National Taiwan University College of Medicine, Taipei, Taiwan, ROC.
authors.forename: Hsiao-Jan
authors.initials: HJ
authors.lastname: Chen
authors.affiliation: Division of Neurosurgery, Department of Surgery, National Taiwan University Hospital, Taiwan, ROC.|Center for Optoelectronic Medicine, National Taiwan University College of Medicine, Taipei, Taiwan, ROC.
authors.forename: Jui-Chang
authors.initials: JC
authors.lastname: Tsai
authors.affiliation: Department of Medical Laboratory Science and Biotechnology, Kaohsiung Medical University, Kaohsiung, Taiwan, ROC.
authors.forename: Sung-Pin
authors.initials: SP
authors.lastname: Tseng
authors.affiliation: Department of Laboratory Medicine, National Taiwan University Hospital, Taipei, Taiwan, ROC.
authors.forename: Tai-Fen
authors.initials: TF
authors.lastname: Lee
authors.affiliation: Department of Laboratory Medicine, National Taiwan University Hospital, Taipei, Taiwan, ROC.
authors.forename: Po-Ren
authors.initials: PR
authors.lastname: Hsueh
authors.affiliation: Institute of Oceanography, National Taiwan University, PO Box 23-13, Taipei, Taiwan, ROC.
authors.forename: Wung Yang
authors.initials: WY
authors.lastname: Shieh
authors.affiliation: Department of Laboratory Medicine, National Taiwan University Hospital, Taipei, Taiwan, ROC.|Department of Clinical Laboratory Sciences and Medical Biotechnology, National Taiwan University College of Medicine, Taipei, Taiwan, ROC.
authors.forename: Lee-Jene
authors.initials: LJ
authors.lastname: Teng
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005260:Female; D058834:Gemella / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D005798:Genes, Bacterial; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D058885:Multilocus Sequence Typing; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013624:Taiwan
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S