title: Environmental fate of double-stranded RNA in agricultural soils.
abstract: A laboratory soil degradation study was conducted to determine the biodegradation potential of a DvSnf7 dsRNA transcript derived from a Monsanto genetically modified (GM) maize product that confers resistance to corn rootworm (CRW; Diabrotica spp.). This study provides new information to improve the environmental assessment of dsRNAs that become pesticidal through an RNAi process. Three agricultural soils differing in their physicochemical characteristics were obtained from the U.S., Illinois (IL; silt loam), Missouri (MO; loamy sand) and North Dakota (ND; clay loam), and exposed to the target dsRNA by incorporating insect-protected maize biomass and purified (in vitro-transcribed) DvSnf7 RNA into soil. The GM and control (non-GM maize) materials were added to each soil and incubated at ca. 22 °C for 48 hours (h). Samples were collected at 12 time intervals during the incubation period, extracted, and analyzed using QuantiGene molecular analysis and insect bioassay methods. The DT50 (half-life) values for DvSnf7 RNA in IL, MO, and ND soils were 19, 28, and 15 h based on QuantiGene, and 18, 29, and 14 h based on insect bioassay, respectively. Furthermore, the DT90 (time to 90% degradation) values for DvSnf7 RNA in all three soils were <35 h. These results indicate that DvSnf7 RNA was degraded and biological activity was undetectable within approximately 2 days after application to soil, regardless of texture, pH, clay content and other soil differences. Furthermore, soil-incorporated DvSnf7 RNA was non-detectable in soil after 48 h, as measured by QuantiGene, at levels ranging more than two orders of magnitude (0.3, 1.5, 7.5 and 37.5 µg RNA/g soil). Results from this study indicate that the DvSnf7 dsRNA is unlikely to persist or accumulate in the environment. Furthermore, the rapid degradation of DvSnf7 dsRNA provides a basis to define relevant exposure scenarios for future RNA-based agricultural products.
authors.affiliation: Regulatory Division, Monsanto Company, St. Louis, Missouri, United States of America.
authors.forename: Samuel
authors.initials: S
authors.lastname: Dubelman
authors.affiliation: Regulatory Division, Monsanto Company, St. Louis, Missouri, United States of America.
authors.forename: Joshua
authors.initials: J
authors.lastname: Fischer
authors.affiliation: Regulatory Division, Monsanto Company, St. Louis, Missouri, United States of America.
authors.forename: Fatima
authors.initials: F
authors.lastname: Zapata
authors.affiliation: Regulatory Division, Monsanto Company, St. Louis, Missouri, United States of America.
authors.forename: Kristin
authors.initials: K
authors.lastname: Huizinga
authors.affiliation: Regulatory Division, Monsanto Company, St. Louis, Missouri, United States of America.
authors.forename: Changjian
authors.initials: C
authors.lastname: Jiang
authors.affiliation: Regulatory Division, Monsanto Company, St. Louis, Missouri, United States of America.
authors.forename: Joshua
authors.initials: J
authors.lastname: Uffman
authors.affiliation: Regulatory Division, Monsanto Company, St. Louis, Missouri, United States of America.
authors.forename: Steven
authors.initials: S
authors.lastname: Levine
authors.affiliation: Regulatory Division, Monsanto Company, St. Louis, Missouri, United States of America.
authors.forename: David
authors.initials: D
authors.lastname: Carson
mesh_terms: D000383:Agriculture; D000818:Animals; D018533:Biomass; D004777:Environment; D006868:Hydrolysis; D007313:Insecta; D007700:Kinetics; D012330:RNA, Double-Stranded / Q000737:chemistry*; D018749:RNA, Plant; D012987:Soil / Q000737:chemistry*
chemical_list: D012330:RNA, Double-Stranded; D018749:RNA, Plant; D012987:Soil