title: Sulforhodamine 101 selectively labels human astrocytoma cells in an animal model of glioblastoma.
abstract: Sulforhodamine 101 (SR101) is a useful tool for immediate staining of astrocytes. We hypothesized that if the selectivity of SR101was maintained in astrocytoma cells, it could prove useful for glioma research. Cultured astrocytoma cells and acute slices from orthotopic human glioma (n=9) and lymphoma (n=6) xenografts were incubated with SR101 and imaged with confocal microscopy. A subset of slices (n=18) were counter-immunostained with glial fibrillary acidic protein and CD20 for stereological assessment of SR101 co-localization. SR101 differentiated astrocytic tumor cells from lymphoma cells. In acute slices, SR101 labeled 86.50% (±1.86; p<0.0001) of astrocytoma cells and 2.19% (±0.47; p<0.0001) of lymphoma cells. SR101-labeled astrocytoma cells had a distinct morphology when compared with in vivo astrocytes. Immediate imaging of human astrocytoma cells in vitro and in ex vivo rodent xenograft tissue labeled with SR101 can identify astrocytic tumor cells and help visualize the tumor margin. These features are useful in studying astrocytoma in the laboratory and may have clinical applications.
authors.affiliation: Division of Neuroscience, Barrow Neurological Institute, St. Joseph's Hospital and Medical Center, Phoenix, AZ, USA; Division of Neurological Surgery, Barrow Neurological Institute, St. Joseph's Hospital and Medical Center, 350 W. Thomas Road, Phoenix, AZ 85013, USA; Arizona College of Osteopathic Medicine, Midwestern University, Glendale, AZ, USA.
authors.forename: Joseph F
authors.initials: JF
authors.lastname: Georges
authors.affiliation: Division of Neurological Surgery, Barrow Neurological Institute, St. Joseph's Hospital and Medical Center, 350 W. Thomas Road, Phoenix, AZ 85013, USA.
authors.forename: Nikolay L
authors.initials: NL
authors.lastname: Martirosyan
authors.affiliation: Division of Neuropathology, Barrow Neurological Institute, St. Joseph's Hospital and Medical Center, Phoenix, AZ, USA.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Eschbacher
authors.affiliation: College of Medicine, University of Arizona, Phoenix, AZ, USA.
authors.forename: Joshua
authors.initials: J
authors.lastname: Nichols
authors.affiliation: Division of Neuroscience, Barrow Neurological Institute, St. Joseph's Hospital and Medical Center, Phoenix, AZ, USA.
authors.forename: Maya
authors.initials: M
authors.lastname: Tissot
authors.affiliation: Division of Neurological Surgery, Barrow Neurological Institute, St. Joseph's Hospital and Medical Center, 350 W. Thomas Road, Phoenix, AZ 85013, USA.
authors.forename: Mark C
authors.initials: MC
authors.lastname: Preul
authors.affiliation: College of Medicine, University of Arizona, Phoenix, AZ, USA.
authors.forename: Burt
authors.initials: B
authors.lastname: Feuerstein
authors.affiliation: College of Medicine, University of Arizona, Phoenix, AZ, USA.
authors.forename: Trent
authors.initials: T
authors.lastname: Anderson
authors.affiliation: Division of Neurological Surgery, Barrow Neurological Institute, St. Joseph's Hospital and Medical Center, 350 W. Thomas Road, Phoenix, AZ 85013, USA.
authors.forename: Robert F
authors.initials: RF
authors.lastname: Spetzler
authors.affiliation: Division of Neurological Surgery, Barrow Neurological Institute, St. Joseph's Hospital and Medical Center, 350 W. Thomas Road, Phoenix, AZ 85013, USA. Electronic address: Neuropub@dignityhealth.org.
authors.forename: Peter
authors.initials: P
authors.lastname: Nakaji
mesh_terms: D000818:Animals; D001254:Astrocytoma / Q000473:pathology*; D001932:Brain Neoplasms / Q000175:diagnosis*; D045744:Cell Line, Tumor; D004396:Coloring Agents; D004195:Disease Models, Animal; D005909:Glioblastoma / Q000175:diagnosis*; D006801:Humans; D008297:Male; D051381:Rats; D012235:Rhodamines; D023041:Xenograft Model Antitumor Assays / Q000379:methods
chemical_list: D004396:Coloring Agents; D012235:Rhodamines; C050829:sulforhodamine 101
keywords: Astrocyte; Astrocytoma; In vivo model confocal microscopy; SR101; Sulforhodamine