title: Purification and culture of corticospinal motor neurons.
abstract: Corticospinal motor neurons (CSMNs) residing in cortical layer V of the mammalian brain project their axons to the spinal cord, where they connect with spinal motor neurons (SMNs) located in the ventral horn of the spinal cord. CSMNs and SMNs control voluntary movements, and their importance becomes obvious in situations where this network breaks down (i.e., in amyotrophic lateral sclerosis [ALS] and after spinal cord injury). Here we provide an overview of recent progress in the anatomical, morphological, and genetic characterization of developing CSMNs, as well as their survival requirements. We also describe model systems used to study CSMNs and introduce an immunopanning procedure for the purification and culture of CSMNs. Although these procedures have so far been used to purify only rodent CSMNs, in principle they should work to purify CSMNs from any vertebrate species, as well any type of central nervous system (CNS) or peripheral nervous system (PNS) neuron that can be retrograde labeled.
authors.affiliation: Laboratory for the Research of Neurodegenerative Diseases, VIB Center for the Biology of Disease, KU Leuven Center for Human Genetics, 3000 Leuven, Belgium.
authors.forename: Wim
authors.initials: W
authors.lastname: Mandemakers
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002469:Cell Separation / Q000379:methods*; D009046:Motor Neurons / Q000502:physiology*; D012377:Rodentia