title: Interactions between neutrophils, Th17 cells, and chemokines during the initiation of experimental model of multiple sclerosis.
abstract: Experimental autoimmune encephalomyelitis (EAE) is an animal model of multiple sclerosis (MS) in which activated T cell and neutrophil interactions lead to neuroinflammation. In this study the expression of CCR6, CXCR2, and CXCR6 in Th17 cells and neutrophils migrating to the brain during EAE was measured, alongside an evaluation of the production of IL-17, IL-23, CCL-20, and CXCL16 in the brain. Next, inflammatory cell subpopulations accumulating in the brain after intracerebral injections of IL-17 or CXCL1, as well as during modulation of EAE with anti-IL-23R or anti-CXCR2 antibodies, were analyzed. Th17 cells upregulate CXCR2 during the preclinical phase of EAE and a significant migration of these cells to the brain was observed. Neutrophils upregulated CCR6, CXCR2, and CXCR6 during EAE, accumulating in the brain both prior to and during acute EAE attacks. Production of IL-17, IL-23, CCL20, and CXCL16 in the CNS was increased during both preclinical and acute EAE. Intracerebral delivery of CXCL1 stimulated the early accumulation of neutrophils in normal and preclinical EAE brains but reduced the migration of Th17 cells to the brain during the preclinical stage of EAE. Modulation of EAE by anti-IL-23R antibodies ameliorated EAE by decreasing the intracerebral accumulation of Th17 cells.
authors.affiliation: Department of Neurology, Epileptology and Stroke, Medical University of Lodz, Ulice Zeromskiego 113, 90 549 Lodz, Poland ; Department of Propedeutics of Neurology, Medical University of Lodz, Ulice Zeromskiego 113, 90 549 Lodz, Poland.
authors.forename: Dagmara Weronika
authors.initials: DW
authors.lastname: Wojkowska
authors.affiliation: Department of Neurology, Epileptology and Stroke, Medical University of Lodz, Ulice Zeromskiego 113, 90 549 Lodz, Poland ; Department of Propedeutics of Neurology, Medical University of Lodz, Ulice Zeromskiego 113, 90 549 Lodz, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Szpakowski
authors.affiliation: Department of Neurology, Epileptology and Stroke, Medical University of Lodz, Ulice Zeromskiego 113, 90 549 Lodz, Poland ; Department of Propedeutics of Neurology, Medical University of Lodz, Ulice Zeromskiego 113, 90 549 Lodz, Poland.
authors.forename: Dominika
authors.initials: D
authors.lastname: Ksiazek-Winiarek
authors.affiliation: Department of Neurology, Epileptology and Stroke, Medical University of Lodz, Ulice Zeromskiego 113, 90 549 Lodz, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Leszczynski
authors.affiliation: Department of Neurology, Epileptology and Stroke, Medical University of Lodz, Ulice Zeromskiego 113, 90 549 Lodz, Poland ; Department of Propedeutics of Neurology, Medical University of Lodz, Ulice Zeromskiego 113, 90 549 Lodz, Poland.
authors.forename: Andrzej
authors.identifier: 0000-0002-1305-1494
authors.initials: A
authors.lastname: Glabinski
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism; D002465:Cell Movement; D018925:Chemokines / Q000276:immunology*; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000378:metabolism / Q000503:physiopathology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005786:Gene Expression Regulation; D007249:Inflammation; D007963:Leukocytes, Mononuclear / Q000166:cytology; D051379:Mice; D009103:Multiple Sclerosis / Q000378:metabolism / Q000503:physiopathology*; D009474:Neurons / Q000473:pathology; D009504:Neutrophils / Q000166:cytology* / Q000378:metabolism; D054399:Receptors, CCR6 / Q000276:immunology; D054387:Receptors, CXCR / Q000276:immunology; D000075744:Receptors, CXCR6; D023063:Receptors, Interleukin-8B / Q000276:immunology; D058504:Th17 Cells / Q000166:cytology*
chemical_list: C498126:CCR6 protein, mouse; D018925:Chemokines; C482906:Cxcr6 protein, mouse; D054399:Receptors, CCR6; D054387:Receptors, CXCR; D000075744:Receptors, CXCR6; D023063:Receptors, Interleukin-8B