title: Resveratrol Prevention of Diabetic Nephropathy Is Associated with the Suppression of Renal Inflammation and Mesangial Cell Proliferation: Possible Roles of Akt/NF-κB Pathway.
abstract: The present study was to investigate the protection of resveratrol (RSV) in diabetes associated with kidney inflammation and cell proliferation. Rat mesangial cell and streptozotocin-induced type 1 diabetes mouse model were used. In vitro, RSV attenuated high glucose-induced plasminogen activator inhibitor (PAI-1) expression and mesangial cell proliferation, as well as Akt and nuclear factor-kappa B (NF- κ B) activation. The similar results were recaptured in the experiment with Akt inhibitors. In vivo, mice were divided into three groups: control group, diabetes mellitus (DM) group, and RSV-treated DM group. Compared with control group, the kidney weight to body weight ratio and albumin to creatinine ratio were increased in DM group, but not in RSV-treated DM group. Furthermore, the increased expression of PAI-1 and intercellular adhesion molecule-1 in diabetic renal cortex were also reduced by RSV administration. Besides, the kidney p-Akt/Akt ratio and NF- κ B were significantly increased in DM group; however, these changes were reversed in RSV-treated DM group. Additionally, immunohistochemistry results indicated that RSV treatment reduced the density of proliferating cell nuclear antigen-positive cells significantly in glomeruli of diabetic mice. These results suggest that RSV prevents diabetes-induced renal inflammation and mesangial cell proliferation possibly through Akt/NF- κ B pathway inhibition.
authors.affiliation: Department of Nephrology, The Second Hospital of Jilin University, 218 Ziqiang Street, Changchun 130041, China.
authors.forename: Feng
authors.identifier: 0000-0002-1538-2908
authors.initials: F
authors.lastname: Xu
authors.affiliation: Department of Cardiology, The Second Hospital of Jilin University, 218 Ziqiang Street, Changchun 130041, China.
authors.forename: Yuehui
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Nephrology, The Second Hospital of Jilin University, 218 Ziqiang Street, Changchun 130041, China.
authors.forename: Wenpeng
authors.initials: W
authors.lastname: Cui
authors.affiliation: Department of Nephrology, The Second Hospital of Jilin University, 218 Ziqiang Street, Changchun 130041, China.
authors.forename: Hang
authors.initials: H
authors.lastname: Yuan
authors.affiliation: Department of Nephrology, The Second Hospital of Jilin University, 218 Ziqiang Street, Changchun 130041, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Sun
authors.affiliation: Department of Nephrology, The Second Hospital of Jilin University, 218 Ziqiang Street, Changchun 130041, China.
authors.forename: Man
authors.initials: M
authors.lastname: Wu
authors.affiliation: Department of Nephrology, The Second Hospital of Jilin University, 218 Ziqiang Street, Changchun 130041, China.
authors.forename: Qiaoyan
authors.initials: Q
authors.lastname: Guo
authors.affiliation: Department of Nephrology, The Second Hospital of Jilin University, 218 Ziqiang Street, Changchun 130041, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Kong
authors.affiliation: Department of Nephrology, The Second Hospital of Jilin University, 218 Ziqiang Street, Changchun 130041, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Wu
authors.affiliation: Department of Nephrology, The Second Hospital of Jilin University, 218 Ziqiang Street, Changchun 130041, China.
authors.forename: Lining
authors.initials: L
authors.lastname: Miao