title: Rapid detection of malaria parasite by toluidine blue method: a new staining method.
abstract: BACKGROUND
  Malaria is a commonest mosquito-borne infectious disease worldwide. Early identification and management of malaria prevents complications and mortality. Identification of the malaria mainly relies on detection of the parasite on blood smears. The present study was conducted to compare Toluidine blue method with Leishman method for detection of malaria parasite and also to study the efficacy and advantages of using Toluidine blue method.
  
  
  METHODS
  In 540 consecutive patients with clinical suspicion of malaria, peripheral smears were prepared. Smears were processed for both conventional Leishman method and Toluidine blue method simultaneously. The significance of Toluidine blue method over Leishman method was analyzed using Chi-square (χ(2)) test.
  
  
  RESULTS
  Out of 540 smears, 28.3% (153/540) were positive for malaria parasite on conventional Leishman method, while the smear positivity was more by Toluidine blue method to 33.3% (180/540) [P value < 0.01]. The remaining 66.67% (360/540) were negative by both Toluidine blue method and conventional Leishman method.
  
  
  CONCLUSIONS
  The Toluidine blue method is simple, rapid, inexpensive, and easily available. The implementation of Toluidine blue method clearly improves microscopic detection of malaria parasite and can be a useful contribution to routine hematology even at rural health sectors.
authors.affiliation: Department of Pathology, Melmaruvathur Adhiparasakti Institute of Medical Sciences and Research, Melmaruvathur, Tamil Nadu, India.
authors.forename: Vamseedhar
authors.initials: V
authors.lastname: Annam
authors.affiliation: Department of Pathology, Melmaruvathur Adhiparasakti Institute of Medical Sciences and Research, Melmaruvathur, Tamil Nadu, India.
authors.forename: Chakkirala Nalini
authors.initials: CN
authors.lastname: Mohan
authors.affiliation: Department of Pathology, Melmaruvathur Adhiparasakti Institute of Medical Sciences and Research, Melmaruvathur, Tamil Nadu, India.
authors.forename: Vazhayil Ramunny
authors.initials: VR
authors.lastname: Mrinalini
keywords: Leishman method; Malaria; Peripheral smear; Toluidine blue method