title: Detection of Helicobacter pylori infection in Egyptian patients with chronic calcular cholecystitis.
abstract: Reports of Helicobacter pylori in biliary tract diseases in humans are very fragmentary, and therefore there is a need for further investigations. This study aims to detect H. pylori in the bile and gall bladder (GB) of patients with chronic calcular cholecystitis (CCC), and to determine the association of H. pylori infection with gallstone type. Thirty patients with CCC admitted for laparoscopic cholecystectomy were investigated, including upper gastro-endoscopy before cholecystectomy. Rapid urease test and histopathological examination were performed on gastric biopsies. The GB specimens were investigated for the presence of H. pylori by immunohistochemistry (IHC). H. pylori antigen in bile was detected by enzyme immunoassay. Chemical analysis of gallstones was performed to determine type. Immunohistochemistry testing showed 73.3% and 66.7% positivity among GB neck and body biopsies, respectively, demonstrating high sensitivity and specificity. A significant association was found between gastric and GB H. pylori positivity (P < 0.01). H. pylori antigen was detected in bile from three CCC cases. The greatest number of stones were of the calcium bilirubinate type. Gall bladder positivity for H. pylori was accompanied by chronic quiescent gastritis (40.9%). In conclusion, H. pylori infection may be an aetiological factor leading to cholecystitis. Gastric colonisation with H. pylori could be a source for GB infection, and the organism may act as a lithogenic component, especially in the context of pure pigmented gallstones.
authors.forename: G F
authors.initials: GF
authors.lastname: Helaly
authors.forename: E F
authors.initials: EF
authors.lastname: El-Ghazzawi
authors.forename: A H
authors.initials: AH
authors.lastname: Kazem
authors.forename: N L
authors.initials: NL
authors.lastname: Dowidar
authors.forename: M M
authors.initials: MM
authors.lastname: Anwar
authors.forename: N M
authors.initials: NM
authors.lastname: Attia
mesh_terms: D000328:Adult; D001646:Bile / Q000382:microbiology; D002764:Cholecystitis / Q000382:microbiology*; D002908:Chronic Disease; D004534:Egypt; D005260:Female; D005704:Gallbladder / Q000382:microbiology; D016481:Helicobacter Infections / Q000175:diagnosis*; D016480:Helicobacter pylori / Q000302:isolation & purification*; D006801:Humans