title: The role of BRAF V600E mutation as a potential marker for prognostic stratification of papillary thyroid carcinoma: a long-term follow-up study.
abstract: Abstract Papillary carcinoma is the most prevalent malignancy of thyroid gland, and its incidence has been recently increased. The BRAF(V600E) mutation is the most frequent genetic alteration in papillary thyroid carcinoma (PTC). The role of BRAF(V600E) mutation as a potential prognostic factor has been controversially reported in different studies, with short-term follow-up. In this study, we evaluated the role of BRAF(V600E) mutation as a potential marker for prognostic stratification of patients with PTC in long-term follow-up. We studied 69 PTC patients with a mean follow-up period of 63.9 months (median: 60 m). The BRAF(V600E) mutation was analyzed by PCR-single-strand conformational polymorphism and sequencing. The correlation between the presence or absence of the BRAF(V600E) mutation, clinicopathological features and prognosis of PTC patients were studied. The BRAF(V600E) mutation was found in 28 of 69 (40.6%) PTC patients, and it was significantly more frequent in older patients (p < 0.001), in advanced tumor stages (p = 0.006) and in patients with history of radiation exposure (p = 0.037). Incomplete response to treatment in PTC patients was significantly correlated with certain clinicopathological characteristics (follow-up time, distant metastases, advanced stage, first thyroglobulin (fTg) level, history of reoperation and external radiotherapy and delay in iodine therapy) but it was not related to the presence of BRAF(V600E) mutation. Prevalence of BRAF(V600E) mutation was 40.6% in patients with papillary thyroid cancer in northeast of Iran. The BRAF(V600E) mutation was associated with older age and advanced tumor stage but was not correlated with incomplete response during follow-up.
authors.affiliation: Surgical Oncology Research Center, Emam Reza Hospital, Faculty of Medicine .
authors.forename: Mahdi
authors.initials: M
authors.lastname: Daliri
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Abbaszadegan
authors.forename: Mostafa Mehrabi
authors.initials: MM
authors.lastname: Bahar
authors.forename: Azadeh
authors.initials: A
authors.lastname: Arabi
authors.forename: Mona
authors.initials: M
authors.lastname: Yadollahi
authors.forename: Azar
authors.initials: A
authors.lastname: Ghafari
authors.forename: Negin
authors.initials: N
authors.lastname: Taghehchian
authors.forename: Seyed Rasoul
authors.initials: SR
authors.lastname: Zakavi
mesh_terms: D000328:Adult; D000367:Age Factors; D019943:Amino Acid Substitution; D002277:Carcinoma / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology / Q000628:therapy; D002291:Carcinoma, Papillary / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology / Q000628:therapy; D005260:Female; D005500:Follow-Up Studies; D056726:Genetic Association Studies; D005819:Genetic Markers; D006801:Humans; D007492:Iran; D008297:Male; D008875:Middle Aged; D009154:Mutation; D009367:Neoplasm Staging; D010044:Outpatient Clinics, Hospital; D011379:Prognosis; D048493:Proto-Oncogene Proteins B-raf / Q000235:genetics* / Q000378:metabolism; D013954:Thyroglobulin / Q000097:blood; D000077273:Thyroid Cancer, Papillary; D013961:Thyroid Gland / Q000201:enzymology / Q000473:pathology* / Q000528:radiation effects; D013964:Thyroid Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology / Q000628:therapy
chemical_list: D005819:Genetic Markers; D013954:Thyroglobulin; C482119:BRAF protein, human; D048493:Proto-Oncogene Proteins B-raf
keywords: BRAFV600E; genetic; mutation; papillary cancer; prognosis; thyroid