title: Composition of essential oil from Tagetes minuta and its cytotoxic, antioxidant and antimicrobial activities.
abstract: The essential oil from the leaves of Tagetes minuta L., growing wild in Yemen, was obtained by hydrodistillation and analyzed by gas chromatography-mass spectrometry. A total of 28 compounds were identified representing 74.2% of total oil composition. Major components of the essential oil were (Z)-ocimenone (15.9%), (E)-ocimenone (34.8%), (Z)-beta-ocimene (8.3%), limonene (2.3%), (Z)-tagetone (1.8%), dihydrotagetone (1.4%) and an unidentified dimethylvinylketone derivative (20.6%). The oil showed moderate cytotoxic activity against MCF-7 breast tumor cells, with an IC50 of 54.7 +/- 6.2 microg/mL. In the DPPH radical scavenging assay, T. minuta oil showed potent antiradical activity with an IC50 value of 36 microg/mL. Antimicrobial activity was also investigated on several microorganisms, and the essential oil exhibited high activity against methicillin-resistant Staphylococcus aureus (MRSA) with an inhibition zone of 23 mm. It also exhibited remarkable antifungal activity against Candida albicans with an inhibition zone of 26 mm.
authors.forename: Nasser A Awadh
authors.initials: NA
authors.lastname: Ali
authors.forename: Farukh S
authors.initials: FS
authors.lastname: Sharopov
authors.forename: Ali G
authors.initials: AG
authors.lastname: Al-Kaf
authors.forename: Gabrielle M
authors.initials: GM
authors.lastname: Hill
authors.forename: Norbert
authors.initials: N
authors.lastname: Arnold
authors.forename: Saeed S
authors.initials: SS
authors.lastname: Al-Sokari
authors.forename: William N
authors.initials: WN
authors.lastname: Setzer
authors.forename: Ludger
authors.initials: L
authors.lastname: Wessjohann
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D061986:MCF-7 Cells; D009822:Oils, Volatile / Q000032:analysis* / Q000494:pharmacology*; D031686:Tagetes / Q000737:chemistry*
chemical_list: D000890:Anti-Infective Agents; D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D009822:Oils, Volatile