title: Prevalence and quantification of Listeria monocytogenes in beef offal at retail level in Selangor, Malaysia.
abstract: A total of 63 beef offal samples (beef liver = 16; beef lung = 14; beef intestine = 9; beef tripe = 15; beef spleen = 9) from three wet markets (A, B, and C) in Selangor, Malaysia were examined for the prevalence and microbial load of Listeria monocytogenes. A combination of the most probable number and polymerase chain reaction (MPN-PCR) method was employed in this study. It was found that L. monocytogenes detected in 33.33% of the beef offal samples. The prevalence of L. monocytogenes in beef offal purchased from wet markets A, B, and C were 22.73%, 37.50% and 41.18% respectively. The density of L. monocytogenes in all the samples ranged from < 3 up to > 2,400 MPN/g. The findings in this study indicate that beef offal can be a potential vehicle of foodborne listeriosis.
authors.affiliation: Food Safety Research Centre, Faculty of Food Science and Technology, University Putra Malaysia, UPM Serdang, Selangor Darul Ehsan, Malaysia.
authors.forename: Chee Hao
authors.initials: CH
authors.lastname: Kuan
authors.affiliation: Food Safety Research Centre, Faculty of Food Science and Technology, University Putra Malaysia, UPM Serdang, Selangor Darul Ehsan, Malaysia.
authors.forename: Woan Chwen
authors.initials: WC
authors.lastname: Wong
authors.affiliation: Food Safety Research Centre, Faculty of Food Science and Technology, University Putra Malaysia, UPM Serdang, Selangor Darul Ehsan, Malaysia.
authors.forename: Chai Fung
authors.initials: CF
authors.lastname: Pui
authors.affiliation: Food Safety Research Centre, Faculty of Food Science and Technology, University Putra Malaysia, UPM Serdang, Selangor Darul Ehsan, Malaysia.
authors.forename: Nor Ainy
authors.initials: NA
authors.lastname: Mahyudin
authors.affiliation: Faculty of Food Technology, Universiti Sultan Zainal Abidin, Kuala Terengganu, Terengganu Darul Iman, Malaysia.
authors.forename: John Yew Huat
authors.initials: JY
authors.lastname: Tang
authors.affiliation: Center for Southeast Asian Studies, Kyoto University, Yoshida Sakyo-ku, Kyoto, Japan.
authors.forename: Mitsuaki
authors.initials: M
authors.lastname: Nishibuchi
authors.affiliation: Food Safety Research Centre, Faculty of Food Science and Technology, University Putra Malaysia, UPM Serdang, Selangor Darul Ehsan, Malaysia.
authors.forename: Son
authors.initials: S
authors.lastname: Radu
mesh_terms: D000818:Animals; D058491:Bacterial Load; D002417:Cattle; D005516:Food Microbiology; D008089:Listeria monocytogenes / Q000302:isolation & purification*; D008296:Malaysia; D016133:Polymerase Chain Reaction; D015995:Prevalence
keywords: Listeria monocytogenes; beef offal; most probable number (MPN); polymerase chain reaction (PCR); prevalence