title: Influence of age on osteoarthritis progression after anterior cruciate ligament transection in rats.
abstract: The anterior cruciate ligament transection (ACLT) model of osteoarthritis (OA) in young rats is widely used to study the pathogenesis of OA and possible treatment approaches. As aging is a key factor in the progression of this condition, it is hypothesized that animals may vary in their responses to ACLT according to their age. The histopathological features of young (2month-old) and middle-aged (12month-old) rats in the presence or absence of ACLT were compared. The results indicated that moderate degradative changes can be detected in the knee joints of sham-operated middle-aged rats compared with young animals. After ACLT, cartilage degradation was significantly higher in middle-aged rats in relation to young animals. An increase in interleukin(IL)-1β and IL-17 suggests the presence of a local inflammatory response represented by synovitis in ACLT rats which is not dependent on age. Our study indicates that age is an important factor affecting the pathogenesis of OA changes after ACLT and it should be considered in studies using this experimental model.
authors.affiliation: Department of Pharmacology and IDM, University of Valencia, Av. Vicent Andrés Estellés s/n, 46100 Burjasot, Valencia, Spain.
authors.forename: María Luisa
authors.initials: ML
authors.lastname: Ferrándiz
authors.affiliation: Department of Pharmacology and IDM, University of Valencia, Av. Vicent Andrés Estellés s/n, 46100 Burjasot, Valencia, Spain.
authors.forename: María Carmen
authors.initials: MC
authors.lastname: Terencio
authors.affiliation: Pre-Clinical R&D Dept, PharmaSci. Div., BIOIBERICA S.A., Barcelona, Spain.
authors.forename: Ramón
authors.initials: R
authors.lastname: Ruhí
authors.affiliation: Pre-Clinical R&D Dept, PharmaSci. Div., BIOIBERICA S.A., Barcelona, Spain.
authors.forename: Josep
authors.initials: J
authors.lastname: Vergés
authors.affiliation: Pre-Clinical R&D Dept, PharmaSci. Div., BIOIBERICA S.A., Barcelona, Spain.
authors.forename: Eulàlia
authors.initials: E
authors.lastname: Montell
authors.affiliation: Pre-Clinical R&D Dept, PharmaSci. Div., BIOIBERICA S.A., Barcelona, Spain.
authors.forename: Anna
authors.initials: A
authors.lastname: Torrent
authors.affiliation: Department of Pharmacology and IDM, University of Valencia, Av. Vicent Andrés Estellés s/n, 46100 Burjasot, Valencia, Spain. Electronic address: maria.j.alcaraz@uv.es.
authors.forename: María José
authors.initials: MJ
authors.lastname: Alcaraz
mesh_terms: D000375:Aging / Q000378:metabolism / Q000473:pathology*; D000818:Animals; D000070598:Anterior Cruciate Ligament Injuries; D001169:Arthritis, Experimental / Q000209:etiology* / Q000378:metabolism / Q000473:pathology; D002358:Cartilage, Articular / Q000473:pathology; D024043:Collagen Type II / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D018450:Disease Progression; D018836:Inflammation Mediators / Q000378:metabolism; D008297:Male; D010003:Osteoarthritis / Q000209:etiology* / Q000378:metabolism / Q000473:pathology; D011509:Proteoglycans / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013585:Synovitis / Q000209:etiology / Q000378:metabolism / Q000473:pathology
chemical_list: D024043:Collagen Type II; D016207:Cytokines; D018836:Inflammation Mediators; D011509:Proteoglycans
keywords: Animal model; Anterior cruciate ligament transection; Middle-aged rats; Osteoarthritis; Young rats