title: Effects of administration of estradiol and progesterone on ovarian stimulation response, and in vitro developmental competence of oocytes in CIDR-treated calves.
abstract: The present study was conducted to investigate the effects of administering estradiol (estradiol-17β, 2.5 mg) plus progesterone (P4, 50 mg) as a part of a controlled internal drug release (CIDR)-based superovulation protocol on ovarian follicles development, and oocyte recovery as well as on in vitro development in prepubertal calves after follicle stimulating hormone (FSH) stimulation. Calves in the treatment group exhibited significantly decreased (P < 0.05) numbers of both small and large antral follicles compared to the control group A and B (<3 mm in diameter [small], 3.4 versus 12.8, 11.6; > 7 mm in diameter [large], 0 versus 7.6, 7.2). The treated animals also exhibited an increased (P < 0.05) proportion of usable oocytes from the total oocytes recovered compared with those of the control group A and B (77% versus 50.2%, 47.8%). Moreover, the rate of cleavage and the percentage of blastocysts displayed an increased trend in the treatment group compared to controls A and B (59.2% versus 50.5%, 50.8%; 15% versus 12.4%, 11.6%, respectively). In conclusion, the application of estradiol-17β plus P4 at CIDR insertion prior to FSH stimulation affected ovarian follicles development and exerted a beneficial effect on the in vitro development of calf oocytes.
authors.affiliation: a Key Laboratory of Animal Genetics, Breeding and Reproduction of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University , Beijing , P. R. China.
authors.forename: Zhenwei
authors.initials: Z
authors.lastname: Jia
authors.forename: Jiaxin
authors.initials: J
authors.lastname: Zhang
authors.forename: Weibin
authors.initials: W
authors.lastname: Zeng
authors.forename: Yulin
authors.initials: Y
authors.lastname: Zhu
authors.forename: Jianhua
authors.initials: J
authors.lastname: Xue
authors.forename: Baihua
authors.initials: B
authors.lastname: Xuan
authors.forename: Jianhui
authors.initials: J
authors.lastname: Tian
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000187:drug effects; D002417:Cattle; D003692:Delayed-Action Preparations / Q000008:administration & dosage / Q000494:pharmacology; D047108:Embryonic Development / Q000187:drug effects; D004958:Estradiol / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D005307:Fertilization in Vitro; D008297:Male; D009865:Oocytes / Q000187:drug effects*; D006080:Ovarian Follicle / Q000187:drug effects*; D010062:Ovulation Induction; D011374:Progesterone / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D003692:Delayed-Action Preparations; D011374:Progesterone; D004958:Estradiol