title: Immunohistochemical analysis of factors related to apoptosis and cellular proliferation in relation to inflammation in dentigerous and odontogenic keratocyst.
abstract: BACKGROUND
  The effect of inflammation on pathogenesis and biological behavior of odontogenic keratocyst (OKC) and dentigerous cyst (DC) is not completely understood. Hence, we aimed to analyze the effect of inflammation on biological behavior of OKC and DC using a proliferative and anti-apoptotic marker, i.e., proliferative cellular nuclear antigen (PCNA) and Bcl-2, respectively.
  
  
  METHODS
  Immunohistochemical staining was performed using anti-PCNA and Bcl-2 antibody in 10 cases each of classical OKC, inflamed OKC and classical DC and inflamed DC.
  
  
  RESULTS
  Inflamed OKC and DC showed a significant increase in PCNA expression and decrease in Bcl-2 expression when compared with non-inflamed cyst. Correlation between inflammation and proliferative and anti-apoptotic activity was found to be statistically non-significant.
  
  
  CONCLUSIONS
  Inflammation is responsible for change in behavior of neoplastic epithelium of OKC and hence should be treated meticulously, whereas in DC it is responsible for changes in the epithelial lining.
authors.affiliation: Department of Oral Pathology, Babu Banarasi Das College of Dental Sciences, Lucknow, Uttar Pradesh, India.
authors.forename: Gadiputi
authors.initials: G
authors.lastname: Sreedhar
authors.affiliation: Department of Pedodontics and Preventive Dentistry, College of Dental Sciences, Amargadh, Bhavnagar, Gujarat, India.
authors.forename: M Vijaya
authors.initials: MV
authors.lastname: Raju
authors.affiliation: Department of Conservative Dentistry and Endodontics, MIDSR Dental College and Hospital, Latur, Maharashtra, India.
authors.forename: Kiran Kumar
authors.initials: KK
authors.lastname: Metta
authors.affiliation: Department of Oral Pathology, MM College of Dental Sciences and Research, Mullana, Amabala, Haryana, India.
authors.forename: Sm
authors.initials: S
authors.lastname: Manjunath
authors.affiliation: Department of Periodontics, RKDF Dental College and Research Centre, Bhopal, India.
authors.forename: Sujan
authors.initials: S
authors.lastname: Shetty
authors.affiliation: Department of Pedodontics, KMCT Dental College Kozhikode, Kerala, India.
authors.forename: Rakesh Kumar
authors.initials: RK
authors.lastname: Agarwal
keywords: Apoptosis; dentigerous cyst; odontogenic cyst; odontogenic keratocyst; proliferative cellular nuclear antigen