title: Characterization of individual mouse cerebrospinal fluid proteomes.
abstract: Analysis of cerebrospinal fluid (CSF) offers key insight into the status of the CNS. Characterization of murine CSF proteomes can provide a valuable resource for studying CNS injury and disease in animal models. However, the small volume of CSF in mice has thus far limited individual mouse proteome characterization. Through nonterminal CSF extractions in C57Bl/6 mice and high-resolution 2D-LC MS/MS analysis of individual murine samples, we report the most comprehensive proteome characterization of individual murine CSF to date. We identified a total of 566 unique proteins, including 128 proteins from three individual CSF samples that have been previously identified in brain tissue. Our methods and analysis provide a mechanism for individual murine CSF proteome analysis. The data are available in the ProteomeXchange with identifier PXD000248 (http://proteomecentral.proteomexchange.org/dataset/PXD000248).
authors.affiliation: Department of Pharmacology, University of Washington, Seattle, WA, USA.
authors.forename: Jeffrey S
authors.initials: JS
authors.lastname: Smith
authors.forename: Thomas E
authors.initials: TE
authors.lastname: Angel
authors.forename: Charles
authors.initials: C
authors.lastname: Chavkin
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Orton
authors.forename: Ronald J
authors.initials: RJ
authors.lastname: Moore
authors.forename: Richard D
authors.initials: RD
authors.lastname: Smith
mesh_terms: D000818:Animals; D002556:Cerebrospinal Fluid Proteins / Q000032:analysis* / Q000737:chemistry / Q000145:classification; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D051379:Mice; D008810:Mice, Inbred C57BL; D020543:Proteome / Q000032:analysis* / Q000737:chemistry; D040901:Proteomics / Q000379:methods*
chemical_list: D002556:Cerebrospinal Fluid Proteins; D020543:Proteome
keywords: Animal proteomics; Cerebrospinal fluid; MS; Mouse; Proteome