title: Effect of Smac and Taxol on non-small-cell lung cancer.
abstract: A series of structurally unique second mitochondria-derived activator of caspases (Smacs) that act as antagonists of the inhibitor of apoptosis proteins (IAPs) directly have been discovered. They play crucial roles in mitochondrial apoptosis pathways and promote chemotherapy-induced apoptosis. In this study, we constructed a eukaryotic expression vector pcDNA3.1/Smac and transfected it into A549 human lung cancer cells. Then we analyzed the cell invasive and cloning ability, as well as cell apoptosis induced by Taxol. The results showed that over-expressed Smac significantly inhibited A549 cell invasive and cloning ability and promoted apoptosis following Taxol treatment. This finding provides a potential approach for the biological therapy of lung cancer.
authors.affiliation: Thoracic Department, The second Hospital of Shandong University, Jinan 250033, China.
authors.forename: Chuanliang
authors.initials: C
authors.lastname: Peng
authors.forename: Yingtao
authors.initials: Y
authors.lastname: Hao
authors.forename: Yunpeng
authors.initials: Y
authors.lastname: Zhao
authors.forename: Qifeng
authors.initials: Q
authors.lastname: Sun
authors.forename: Xiaogang
authors.initials: X
authors.lastname: Zhao
authors.forename: Bo
authors.initials: B
authors.lastname: Cong
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D051017:Apoptosis Regulatory Proteins; D001483:Base Sequence; D002289:Carcinoma, Non-Small-Cell Lung / Q000473:pathology*; D045744:Cell Line, Tumor; D017931:DNA Primers; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000502:physiology*; D008175:Lung Neoplasms / Q000473:pathology*; D024101:Mitochondrial Proteins / Q000502:physiology*; D009361:Neoplasm Invasiveness; D017239:Paclitaxel / Q000494:pharmacology*; D010957:Plasmids; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D051017:Apoptosis Regulatory Proteins; C412403:DIABLO protein, human; D017931:DNA Primers; D047908:Intracellular Signaling Peptides and Proteins; D024101:Mitochondrial Proteins; D017239:Paclitaxel
keywords: apoptosis; chemosensitivity; cloning ability; invasive ability; non-small-cell lung cancer; second mitochondria-derived activator of caspase