title: Functional ultrasound imaging reveals different odor-evoked patterns of vascular activity in the main olfactory bulb and the anterior piriform cortex.
abstract: Topographic representation of the outside world is a key feature of sensory systems, but so far it has been difficult to define how the activity pattern of the olfactory information is distributed at successive stages in the olfactory system. We studied odor-evoked activation patterns in the main olfactory bulb and the anterior piriform cortex of rats using functional ultrasound (fUS) imaging. fUS imaging is based on the use of ultrafast ultrasound scanners and detects variations in the local blood volume during brain activation. It makes deep brain imaging of ventral structures, such as the piriform cortex, possible. Stimulation with two different odors (hexanal and pentylacetate) induced the activation of odor-specific zones that were spatially segregated in the main olfactory bulb. Interestingly, the same odorants triggered the activation of the entire anterior piriform cortex, in all layers, with no distinguishable odor-specific areas detected in the power Doppler images. These fUS imaging results confirm the spatial distribution of odor-evoked activity in the main olfactory bulb, and furthermore, they reveal the absence of such a distribution in the anterior piriform cortex at the macroscopic scale in vivo.
authors.affiliation: Institut Langevin, ESPCI ParisTech, CNRS UMR7587, Inserm U979, Université Paris Diderot 7, 1 rue Jussieu, 75005 Paris, France.
authors.forename: B F
authors.initials: BF
authors.lastname: Osmanski
authors.affiliation: Laboratoire d'Imagerie et Modélisation en Neurobiologie et Cancérologie, CNRS UMR8165, Université Paris Diderot 7 et Université Paris Sud, 91405 Orsay France.
authors.forename: C
authors.initials: C
authors.lastname: Martin
authors.affiliation: Institut Langevin, ESPCI ParisTech, CNRS UMR7587, Inserm U979, Université Paris Diderot 7, 1 rue Jussieu, 75005 Paris, France.
authors.forename: G
authors.initials: G
authors.lastname: Montaldo
authors.affiliation: Laboratoire d'Imagerie et Modélisation en Neurobiologie et Cancérologie, CNRS UMR8165, Université Paris Diderot 7 et Université Paris Sud, 91405 Orsay France.
authors.forename: P
authors.initials: P
authors.lastname: Lanièce
authors.affiliation: Laboratoire d'Imagerie et Modélisation en Neurobiologie et Cancérologie, CNRS UMR8165, Université Paris Diderot 7 et Université Paris Sud, 91405 Orsay France.
authors.forename: F
authors.initials: F
authors.lastname: Pain
authors.affiliation: Institut Langevin, ESPCI ParisTech, CNRS UMR7587, Inserm U979, Université Paris Diderot 7, 1 rue Jussieu, 75005 Paris, France.
authors.forename: M
authors.initials: M
authors.lastname: Tanter
authors.affiliation: Laboratoire d'Imagerie et Modélisation en Neurobiologie et Cancérologie, CNRS UMR8165, Université Paris Diderot 7 et Université Paris Sud, 91405 Orsay France. Electronic address: gurden@imnc.in2p3.fr.
authors.forename: H
authors.initials: H
authors.lastname: Gurden
mesh_terms: D000818:Animals; D001931:Brain Mapping; D008297:Male; D009812:Odorants; D009830:Olfactory Bulb / Q000098:blood supply / Q000000981:diagnostic imaging / Q000502:physiology*; D066195:Piriform Cortex / Q000098:blood supply / Q000000981:diagnostic imaging / Q000502:physiology*; D051381:Rats; D020318:Rats, Long-Evans; D014463:Ultrasonography
keywords: Blood volume; Functional ultrasound imaging; Odor activation; Piriform cortex