title: Extended storage of buffy coat platelet concentrates in plasma or a platelet additive solution.
abstract: BACKGROUND
  Platelet (PLT) concentrates (PCs) prepared from whole blood in the United States are made using the PLT-rich plasma method. The PCs must be made within 8 hours of blood collection and stored for only 5 days. In Europe and Canada, PCs are made using the buffy coat (BC) method from whole blood held overnight at 22 °C and storage times may be up to 7 days. Our studies were designed to determine how long BC PLTs can be stored in plasma or Plasmalyte while meeting the FDA's poststorage viability criteria.
  
  
  METHODS
  Normal subjects donated whole blood that was stored at 22 °C for 22 ± 2 hours before preparation of BC PLTs. PLTs were stored for 5 to 8 days in either plasma or Plasmalyte concentrations of 65 or 80%. Radiolabeled autologous stored versus fresh PLT recoveries and survivals were assessed as well as poststorage in vitro assays.
  
  
  RESULTS
  BC PLTs stored in either plasma or 65% Plasmalyte met FDA poststorage PLT recovery criteria for 7 days but survivals for only 6 days, while storage in 80% Plasmalyte gave very poor results. Both stored PLT recoveries and survivals correlated with the same donor's fresh results, but the correlation was much stronger between recoveries than survivals. In vitro measures of extent of shape change, morphology score, and pH best predicted poststorage PLT recoveries, while annexin V binding best predicted PLT survivals.
  
  
  CONCLUSIONS
  BC PLTs stored in either plasma or 65% Plasmalyte meet FDA's poststorage viability criteria for 6 days.
authors.affiliation: Puget Sound Blood Center, Seattle, Washington; University of Washington School of Medicine, Seattle, Washington.
authors.forename: Sherrill J
authors.initials: SJ
authors.lastname: Slichter
authors.forename: Doug
authors.initials: D
authors.lastname: Bolgiano
authors.forename: Jill
authors.initials: J
authors.lastname: Corson
authors.forename: Mary Kay
authors.initials: MK
authors.lastname: Jones
authors.forename: Todd
authors.initials: T
authors.lastname: Christoffel
authors.forename: S Lawrence
authors.initials: SL
authors.lastname: Bailey
authors.forename: Esther
authors.initials: E
authors.lastname: Pellham
mesh_terms: D001792:Blood Platelets / Q000166:cytology*; D001793:Blood Preservation / Q000379:methods*; D002470:Cell Survival / Q000502:physiology; D006801:Humans; D053657:Platelet-Rich Plasma / Q000166:cytology; D013997:Time Factors