title: An evaluation of multiple phenotypic screening methods for Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacteriaceae.
abstract: Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacteriaceae may display MICs to carbapenems within susceptible or intermediate ranges, prompting confirmatory testing. Four phenotypic methods to detect KPC producers were evaluated against a collection of clinical Enterobacteriaceae isolates. Meropenem-phenylboronic acid double disk synergy testing demonstrated the best performance with 100% sensitivity and specificity.
authors.affiliation: Northeastern University School of Pharmacy, Boston, MA, USA. Electronic address: e.hirsch@neu.edu.
authors.forename: Elizabeth B
authors.initials: EB
authors.lastname: Hirsch
authors.affiliation: University of Houston College of Pharmacy, Houston, TX, USA.
authors.forename: Kai-Tai
authors.initials: KT
authors.lastname: Chang
authors.affiliation: Northeastern University School of Pharmacy, Boston, MA, USA.
authors.forename: Paola C
authors.initials: PC
authors.lastname: Zucchi
authors.affiliation: Northeastern University School of Pharmacy, Boston, MA, USA.
authors.forename: Dennis N
authors.initials: DN
authors.lastname: Francoeur
authors.affiliation: University of Houston College of Pharmacy, Houston, TX, USA.
authors.forename: Kimberly R
authors.initials: KR
authors.lastname: Ledesma
authors.affiliation: University of Houston College of Pharmacy, Houston, TX, USA; St. Luke's Episcopal Hospital, Houston, TX, USA.
authors.forename: Vincent H
authors.initials: VH
authors.lastname: Tam
authors.affiliation: St. Luke's Episcopal Hospital, Houston, TX, USA.
authors.forename: Todd M
authors.initials: TM
authors.lastname: Lasco
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000096:biosynthesis*; D015373:Bacterial Typing Techniques / Q000379:methods*; D001897:Boronic Acids; D006801:Humans; D007710:Klebsiella Infections / Q000382:microbiology; D007711:Klebsiella pneumoniae / Q000145:classification* / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D008826:Microbial Sensitivity Tests; D010641:Phenotype; D012680:Sensitivity and Specificity; D018440:beta-Lactam Resistance / Q000235:genetics*; D001618:beta-Lactamases / Q000096:biosynthesis*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D001897:Boronic Acids; D001618:beta-Lactamases; C063912:carbapenemase; C010686:benzeneboronic acid
keywords: CRE; Carbapenem; Chromogenic agar; Modified Hodge test; Phenylboronic acid