title: The retrovirus HERVH is a long noncoding RNA required for human embryonic stem cell identity.
abstract: Human endogenous retrovirus subfamily H (HERVH) is a class of transposable elements expressed preferentially in human embryonic stem cells (hESCs). Here, we report that the long terminal repeats of HERVH function as enhancers and that HERVH is a nuclear long noncoding RNA required to maintain hESC identity. Furthermore, HERVH is associated with OCT4, coactivators and Mediator subunits. Together, these results uncover a new role of species-specific transposable elements in hESCs.
authors.affiliation: Gene Regulation Laboratory, Genome Institute of Singapore, Singapore.
authors.forename: Xinyi
authors.initials: X
authors.lastname: Lu
authors.affiliation: 1] Gene Regulation Laboratory, Genome Institute of Singapore, Singapore. [2] Department of Biochemistry, National University of Singapore, Singapore.
authors.forename: Friedrich
authors.initials: F
authors.lastname: Sachs
authors.affiliation: Department of Human Genetics, McGill University, Montréal, Québec, Canada.
authors.forename: LeeAnn
authors.initials: L
authors.lastname: Ramsay
authors.affiliation: 1] Gene Regulation Laboratory, Genome Institute of Singapore, Singapore. [2] Département de Biologie, Université de Sherbrooke, Sherbrooke, Québec, Canada.
authors.forename: Pierre-Étienne
authors.initials: PÉ
authors.lastname: Jacques
authors.affiliation: Gene Regulation Laboratory, Genome Institute of Singapore, Singapore.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Göke
authors.affiliation: 1] Department of Human Genetics, McGill University, Montréal, Québec, Canada. [2] Génome Québec Innovation Center, McGill University, Montréal, Québec, Canada.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Bourque
authors.affiliation: 1] Gene Regulation Laboratory, Genome Institute of Singapore, Singapore. [2] Department of Biochemistry, National University of Singapore, Singapore. [3] Department of Biological Sciences, National University of Singapore, Singapore. [4] School of Biological Sciences, Nanyang Technological University, Singapore.
authors.forename: Huck-Hui
authors.initials: HH
authors.lastname: Ng
mesh_terms: D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism*; D020077:Endogenous Retroviruses / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D005786:Gene Expression Regulation; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D050814:Octamer Transcription Factor-3 / Q000378:metabolism; D039904:Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism*; D062085:RNA, Long Noncoding / Q000032:analysis / Q000737:chemistry / Q000502:physiology*; D013045:Species Specificity
chemical_list: D050814:Octamer Transcription Factor-3; C494173:POU5F1 protein, human; D062085:RNA, Long Noncoding