title: Preliminary studies of the effects of psychological stress on circulating lymphocytes analyzed by synchrotron radiation based-Fourier transform infrared microspectroscopy.
abstract: Psychological stress is a condition that not only generates behavioral disorders but also disrupts homeostasis and immune activity that can exacerbate or lead to inflammatory diseases. The aim of this work was to study biochemical changes in circulating immune cells from rats under psychological stress by using vibrational spectroscopy. A stress model was used, where exposure to a stressor was repeated for 5 days. Subsequently, circulating lymphocytes were examined for their biomolecular vibrational fingerprints with synchrotron radiation based-Fourier transform infrared microspectroscopy. The results showed an increased absorption at the ester lipid region (1720-1755 cm(-1)) in lymphocytes from stressed rats, suggesting lipid peroxidation. Statistical significant changes in wavenumber peak position and absorbance in the nucleic acid region were also observed (915-950 cm(-1) Z-DNA, 1090-1150 cm(-1) symmetric stretching of P-O-C, 1200-1260 cm(-1) asymmetric PO2 and 1570-1510 cm(-1) methylated nucleotides) which suggest a reduction of transcriptional activity in lymphocytes from stressed rat. These results unravel part of the mechanisms by which psychological stress may affect the immune system leading to systemic consequences.
authors.affiliation: Escuela Nacional de Medicina y Homeopatía, Instituto Politécnico Nacional, Guillermo Massieu Helguera, No. 239, Fraccionamiento "La Escalera", Ticomán, C.P. 07320 México DF, Mexico; Departamento de Ciencias Químico Biológicas, Instituto de Ciencias Biomédicas, Universidad Autónoma de Ciudad Juárez, Anillo envolvente Pronaf y Estocolmo s/n, 32310 Cd. Juárez, Mexico. Electronic address: alejandra.vargas@uacj.mx.
authors.forename: Alejandra
authors.initials: A
authors.lastname: Vargas-Caraveo
authors.affiliation: ID21, European Synchrotron Radiation Facility, 6 Rue Jules Horowitz, 38000 Grenoble, France. Electronic address: hiram.castillo_michel@esrf.fr.
authors.forename: Hiram
authors.initials: H
authors.lastname: Castillo-Michel
authors.affiliation: Escuela Nacional de Medicina y Homeopatía, Instituto Politécnico Nacional, Guillermo Massieu Helguera, No. 239, Fraccionamiento "La Escalera", Ticomán, C.P. 07320 México DF, Mexico; Departamento de Ciencias Químico Biológicas, Instituto de Ciencias Biomédicas, Universidad Autónoma de Ciudad Juárez, Anillo envolvente Pronaf y Estocolmo s/n, 32310 Cd. Juárez, Mexico. Electronic address: gloria.mejia@uacj.mx.
authors.forename: Gloria Erika
authors.initials: GE
authors.lastname: Mejia-Carmona
authors.affiliation: Escuela Nacional de Medicina y Homeopatía, Instituto Politécnico Nacional, Guillermo Massieu Helguera, No. 239, Fraccionamiento "La Escalera", Ticomán, C.P. 07320 México DF, Mexico. Electronic address: dperez@ipn.mx.
authors.forename: David Guillermo
authors.initials: DG
authors.lastname: Pérez-Ishiwara
authors.affiliation: ID21, European Synchrotron Radiation Facility, 6 Rue Jules Horowitz, 38000 Grenoble, France. Electronic address: marine.cotte@esrf.fr.
authors.forename: Marine
authors.initials: M
authors.lastname: Cotte
authors.affiliation: Departamento de Ciencias Químico Biológicas, Instituto de Ciencias Biomédicas, Universidad Autónoma de Ciudad Juárez, Anillo envolvente Pronaf y Estocolmo s/n, 32310 Cd. Juárez, Mexico. Electronic address: alejandro.martinez@uacj.mx.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Martínez-Martínez
mesh_terms: D000818:Animals; D008214:Lymphocytes / Q000276:immunology* / Q000473:pathology; D008563:Membrane Lipids / Q000276:immunology*; D051381:Rats; D017208:Rats, Wistar; D017550:Spectroscopy, Fourier Transform Infrared; D013315:Stress, Psychological / Q000276:immunology* / Q000473:pathology; D017356:Synchrotrons; D014158:Transcription, Genetic / Q000276:immunology*
chemical_list: D008563:Membrane Lipids
keywords: Chronic stress; Lipid peroxidation; Predator odor; Psychoneuroimmunology; ROS; Z-DNA