title: Effect of ionic liquids on the different hierarchical order of type I collagen.
abstract: The effect of ionic liquids (ILs) on proteins has been gaining huge interest due to easy tunability of cation and anion for generating the desired effect. This study explores the effect of alkyl imidazolium chloride ILs on collagen at molecular, inter-fibrillar and skin matrix level. Circular dichroic studies reveal that at the molecular level, the secondary structure of collagen was not affected by imidazolium ILs and there was no change in thermal stability as well. However, collagen at the inter-fibrillar level behaved differently. With increase in concentration of ILs, remarkable decrease in thermal stability of rat tail tendon (RTT) collagen fibers with marginal swelling effect was seen. SEM micrographs of skin matrix treated with IL show opening up of pores. This kind of exquisite behavior of ILs at different hierarchal order of collagen indicates that ILs are endowed with potential lyotropic action, which can be judiciously employed for biomedical applications.
authors.affiliation: Chemical Laboratory, CSIR-Central Leather Research Institute, Adyar, Chennai 600020, India.
authors.forename: Ami
authors.initials: A
authors.lastname: Mehta
authors.affiliation: Chemical Laboratory, CSIR-Central Leather Research Institute, Adyar, Chennai 600020, India.
authors.forename: J Raghava
authors.initials: JR
authors.lastname: Rao
authors.affiliation: Chemical Laboratory, CSIR-Central Leather Research Institute, Adyar, Chennai 600020, India. Electronic address: nishad@clri.res.in.
authors.forename: Nishter Nishad
authors.initials: NN
authors.lastname: Fathima
mesh_terms: D000818:Animals; D024042:Collagen Type I / Q000737:chemistry* / Q000187:drug effects*; D006041:Goats; D007093:Imidazoles / Q000494:pharmacology; D052578:Ionic Liquids / Q000494:pharmacology*; D016062:Porosity; D055550:Protein Stability / Q000187:drug effects; D017433:Protein Structure, Secondary; D051381:Rats; D012867:Skin / Q000187:drug effects / Q000648:ultrastructure; D017550:Spectroscopy, Fourier Transform Infrared; D013623:Tail; D013696:Temperature; D013710:Tendons / Q000737:chemistry
chemical_list: D024042:Collagen Type I; D007093:Imidazoles; D052578:Ionic Liquids; C029899:imidazole
keywords: Circular dichroism (CD); Collagen; Imidazolium chloride; Lyotropic action; Room temperature ionic liquids; Scanning electron microscopy (SEM)