title: Recent advances in rational approaches for enzyme engineering.
abstract: Enzymes are an attractive alternative in the asymmetric syntheses of chiral building blocks. To meet the requirements of industrial biotechnology and to introduce new functionalities, the enzymes need to be optimized by protein engineering. This article specifically reviews rational approaches for enzyme engineering and de novo enzyme design involving structure-based approaches developed in recent years for improvement of the enzymes' performance, broadened substrate range, and creation of novel functionalities to obtain products with high added value for industrial applications.
authors.affiliation: ACIB GmbH, (Austrian Centre of Industrial Biotechnology), c/o TU Graz, 8010 Graz, Austria.
authors.forename: Kerstin
authors.initials: K
authors.lastname: Steiner
authors.affiliation: ACIB GmbH, (Austrian Centre of Industrial Biotechnology), c/o TU Graz, 8010 Graz, Austria ; Institute of Molecular Biotechnology, TU Graz, 8010 Graz, Austria.
authors.forename: Helmut
authors.initials: H
authors.lastname: Schwab
keywords: artificial metalloenzymes; de novo enzyme design; promiscuous enzymes; protein engineering; rational design; structure-guided engineering