title: Lactonase-expressing Lactobacillus plantarum NC8 attenuates the virulence factors of multiple drug resistant Pseudomonas aeruginosa in co-culturing environment.
abstract: Pseudomonas aeruginosa possesses an arcade of both cell-associated and extracellular cytotoxic virulence factors which are regulated by a multi-component quorum sensing system. Many research studies report success of lactonase in combating the pathogenicity of P. aeruginosa but delivery of lactonase remains a challenge. The present study aims at developing a delivery vehicle for lactonase. Lactobacillus plantarum NC8 was used as host for aiiA (Bacillus thuringiensis 4A3 lactonase gene) using pSIP409 expression vector. pSIP409: aiiA construct was stably maintained in L. plantarum NC8. Co-culturing of multi-drug resistant (MDR) clinical isolates of P. aeruginosa and PAO1 with recombinant L. plantarum NC8 led to significant reduction (p < 0.001) in extracellular virulence factors like pyocyanin, protease, elastase and rhamnolipids in P. aeruginosa and also showed significant reduction in adhesion of P. aeruginosa strains to uroepithelial cells in vitro. This study shows the heterologous expression of AiiA lactonase in L. plantarum NC8. Co-culturing of lactonase expressing L. plantarum NC8 with MDR P. aeruginosa strains led to attenuation of their virulence significantly. These results underscore the potential application of recombinant L. plantarum NC8 with anti-quorum sensing properties to control infections caused by multidrug resistant P. aeruginosa.
authors.affiliation: Department of Biotechnology, Panjab University, Chandigarh, 160014, India, dsusiin@gmail.com.
authors.forename: Sudha
authors.initials: S
authors.lastname: Joshi
authors.forename: Amanjot
authors.initials: A
authors.lastname: Kaur
authors.forename: Prince
authors.initials: P
authors.lastname: Sharma
authors.forename: Kusum
authors.initials: K
authors.lastname: Harjai
authors.forename: Neena
authors.initials: N
authors.lastname: Capalash
mesh_terms: D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000378:metabolism / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects; D018920:Coculture Techniques / Q000379:methods; D024901:Drug Resistance, Multiple, Bacterial / Q000187:drug effects; D005260:Female; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D006801:Humans; D048191:Lactiplantibacillus plantarum / Q000201:enzymology / Q000235:genetics*; D008666:Metalloendopeptidases / Q000378:metabolism / Q000494:pharmacology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000302:isolation & purification; D053038:Quorum Sensing / Q000187:drug effects; D014552:Urinary Tract Infections / Q000188:drug therapy / Q000382:microbiology*; D014774:Virulence / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; C406735:AiiA protein, Bacillus; D008666:Metalloendopeptidases