title: Protective Effect of Solanum nigrum Leaves Extract on Immobilization Stress Induced Changes in Rat's Brain.
abstract: The prophylactic or curative antioxidant efficacy of crude extract and the active constituent of S. nigrum leaves were evaluated in modulating inherent antioxidant system altered due to immobilization stress in rat brain tissues, in terms of measurement of glutathione (GSH), lipid peroxidation (thiobarbituric acid reactive substances, TBARS), and free radical scavenging enzymes activities. Rats were treated with single dose of crude extract of S. nigrum prior to and after 6 h of immobilization stress exposure. Exposure to immobilization stress resulted in a decrease in the brain levels of glutathione, SOD, GST, and catalase, with an increase in thiobarbituric acid reactive substances (TBARS) levels. Treatment of S. nigrum extract and its active constituents to both pre- and poststressed rats resulted in significant modulation in the above mentioned parameters towards their control values with a relative dominance by the latter. Brain is vulnerable to stress induced prooxidant insult due to high levels of fat content. Thus, as a safe herbal medication the S. nigrum leaves extract or its isolated constituents can be used as nutritional supplement for scavenging free radicals generated in the brain due to physical or psychological stress or any neuronal diseases per se.
authors.affiliation: Center of Excellence in Genomic Medicine Research, King Abdulaziz University, Jeddah, Saudi Arabia.
authors.forename: Syed Kashif
authors.initials: SK
authors.lastname: Zaidi
authors.affiliation: Department of Neurology, Georgia Regents University, Augusta, GA 30912, USA.
authors.forename: Md Nasrul
authors.initials: MN
authors.lastname: Hoda
authors.affiliation: King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia.
authors.forename: Shams
authors.initials: S
authors.lastname: Tabrez
authors.affiliation: Center of Excellence in Genomic Medicine Research, King Abdulaziz University, Jeddah, Saudi Arabia.
authors.forename: Shakeel Ahmed
authors.initials: SA
authors.lastname: Ansari
authors.affiliation: Center of Excellence in Genomic Medicine Research, King Abdulaziz University, Jeddah, Saudi Arabia.
authors.forename: Mohammad Alam
authors.initials: MA
authors.lastname: Jafri
authors.affiliation: Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia.
authors.forename: Mohd
authors.initials: M
authors.lastname: Shahnawaz Khan
authors.affiliation: Department of Surgery, Burn and Shock Trauma Research Institute, Loyola University, 1032 W. Sheridan Road, Chicago, IL 60660, USA.
authors.forename: Shirin
authors.initials: S
authors.lastname: Hasan
authors.affiliation: Center of Excellence in Genomic Medicine Research, King Abdulaziz University, Jeddah, Saudi Arabia.
authors.forename: Mohammed H
authors.initials: MH
authors.lastname: Alqahtani
authors.affiliation: Center of Excellence in Genomic Medicine Research, King Abdulaziz University, Jeddah, Saudi Arabia ; Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia.
authors.forename: Adel
authors.initials: A
authors.lastname: Mohammed Abuzenadah
authors.affiliation: College of Medical Rehabilitation, Qassim University, Buraydah, Saudi Arabia.
authors.forename: Naheed
authors.initials: N
authors.lastname: Banu