title: Glycosaminoglycan mimetic improves enrichment and cell functions of human endothelial progenitor cell colonies.
abstract: Human circulating endothelial progenitor cells isolated from peripheral blood generate in culture cells with features of endothelial cells named late-outgrowth endothelial colony-forming cells (ECFC). In adult blood, ECFC display a constant quantitative and qualitative decline during life span. Even after expansion, it is difficult to reach the cell dose required for cell therapy of vascular diseases, thus limiting the clinical use of these cells. Glycosaminoglycans (GAG) are components from the extracellular matrix (ECM) that are able to interact and potentiate heparin binding growth factor (HBGF) activities. According to these relevant biological properties of GAG, we designed a GAG mimetic having the capacity to increase the yield of ECFC production from blood and to improve functionality of their endothelial outgrowth. We demonstrate that the addition of [OTR(4131)] mimetic during the isolation process of ECFC from Cord Blood induces a 3 fold increase in the number of colonies. Moreover, addition of [OTR(4131)] to cell culture media improves adhesion, proliferation, migration and self-renewal of ECFC. We provide evidence showing that GAG mimetics may have great interest for cell therapy applied to vascular regeneration therapy and represent an alternative to exogenous growth factor treatments to optimize potential therapeutic properties of ECFC.
authors.affiliation: EAC CNRS 7149, Laboratoire CRRET, Université Paris Est Créteil, Faculté des Sciences et Technologies, 61 Avenue du Général de Gaulle, 94010 Créteil Cedex, France; INSERM U972, Hôpital Paul Brousse, Villejuif, France. Electronic address: fabien.che@gmail.com.
authors.forename: Fabien
authors.initials: F
authors.lastname: Chevalier
authors.affiliation: ABCell-Bio, 6 rue Pierre Haret, 75009 Paris, France. Electronic address: melanie.lavergne@abcell-bio.com.
authors.forename: Mélanie
authors.initials: M
authors.lastname: Lavergne
authors.affiliation: INSERM U972, Hôpital Paul Brousse, Villejuif, France. Electronic address: elisa.negroni@upmc.fr.
authors.forename: Elisa
authors.initials: E
authors.lastname: Negroni
authors.affiliation: INSERM U972, Hôpital Paul Brousse, Villejuif, France. Electronic address: segolene.ferratge@inserm.fr.
authors.forename: Ségolène
authors.initials: S
authors.lastname: Ferratge
authors.affiliation: EAC CNRS 7149, Laboratoire CRRET, Université Paris Est Créteil, Faculté des Sciences et Technologies, 61 Avenue du Général de Gaulle, 94010 Créteil Cedex, France. Electronic address: carpentier@u-pec.fr.
authors.forename: Gilles
authors.initials: G
authors.lastname: Carpentier
authors.affiliation: OTR3 company, 4 rue Française, 75001 Paris, France. Electronic address: marie.gilbert@otr3.com.
authors.forename: Marie
authors.initials: M
authors.lastname: Gilbert-Sirieix
authors.affiliation: OTR3 company, 4 rue Française, 75001 Paris, France. Electronic address: fernando.sineriz@otr3.com.
authors.forename: Fernando
authors.initials: F
authors.lastname: Siñeriz
authors.affiliation: INSERM U972, Hôpital Paul Brousse, Villejuif, France. Electronic address: georges.uzan@inserm.fr.
authors.forename: Georges
authors.initials: G
authors.lastname: Uzan
authors.affiliation: EAC CNRS 7149, Laboratoire CRRET, Université Paris Est Créteil, Faculté des Sciences et Technologies, 61 Avenue du Général de Gaulle, 94010 Créteil Cedex, France. Electronic address: albanese@u-pec.fr.
authors.forename: Patricia
authors.initials: P
authors.lastname: Albanese
mesh_terms: D002448:Cell Adhesion; D002465:Cell Movement; D049109:Cell Proliferation; D003114:Colony-Forming Units Assay; D066026:Endothelial Progenitor Cells / Q000166:cytology* / Q000378:metabolism*; D005260:Female; D005312:Fetal Blood / Q000166:cytology; D006025:Glycosaminoglycans / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D007231:Infant, Newborn; D008297:Male
chemical_list: D006025:Glycosaminoglycans