title: Clinical usefulness of the PAXgene™ bone marrow RNA system for stabilizing total RNA.
abstract: The collection of clinical samples, such as bone marrow (BM) and peripheral blood, is an important procedure for the extraction of the cellular RNA. It is essential to preserve the extracted RNA during and after the collection of clinical samples to ensure the accurate analysis of gene expression. To date, the PAXgene™ Blood RNA System has been proven useful for stabilizing RNA extracted from peripheral blood; however, a problem concerning the stability of the total RNA stored using the system has been identified. The PAXgene™ Bone Marrow RNA System (BM system) is a newly developed system, and its clinical usefulness as a stabilizer for the cellular RNA in BM and peripheral blood was investigated with respect to the quality of RNA extracted using this system. A quantitative reverse transcriptase polymerase chain reaction (RT-PCR) was carried out using total RNA extracted with the BM system, which showed that total RNA was more stable in the BM system than in the conventional system, indicating that the BM system can be applied to RT-PCR. The BM system enabled us to detect Wilms' tumor suppressor gene (WT1) more effectively than the conventional system. In conclusion, the BM system is clinically valuable for extracting and stabilizing total RNA of high quality.
authors.affiliation: Department of Clinical Laboratory Science, Faculty of Health Care, Tenri Health Care University, Tenri, Japan; Department of Biomedical Informatics, Division of Health Sciences, Graduate School of Medicine, Osaka University, Suita, Japan.
authors.forename: Mayu
authors.initials: M
authors.lastname: Takeda
authors.forename: Tadao
authors.initials: T
authors.lastname: Funato
authors.forename: Masaki
authors.initials: M
authors.lastname: Ikemoto
authors.forename: Toru
authors.initials: T
authors.lastname: Nanmoku
authors.forename: Yoshimasa
authors.initials: Y
authors.lastname: Urasaki
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Iwatani
mesh_terms: D001800:Blood Specimen Collection / Q000295:instrumentation / Q000379:methods*; D001853:Bone Marrow / Q000378:metabolism*; D001856:Bone Marrow Examination / Q000379:methods; D045744:Cell Line, Tumor; D004586:Electrophoresis; D015870:Gene Expression / Q000502:physiology; D006801:Humans; D007951:Leukemia, Myeloid / Q000175:diagnosis* / Q000235:genetics; D016133:Polymerase Chain Reaction; D012313:RNA / Q000235:genetics / Q000378:metabolism*; D020871:RNA Stability; D012334:RNA, Neoplasm / Q000032:analysis; D013053:Spectrophotometry; D025721:WT1 Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D012334:RNA, Neoplasm; D025721:WT1 Proteins; D012313:RNA
keywords: RIN; RNA electropherogram; WT1 gene; gene expression; real-time quantitative PCR