title: NOD2/CARD15 and IL23R genetic variability in 204 Algerian Crohn's disease.
abstract: NOD2/CARD15 and IL23R gene variants play an important role in the susceptibility to Crohn's disease (CD). Studies of genotype-phenotype relationship suggest that these variants are associated with the development of the disease and specific phenotype. Preliminary reports analyzing the association between these variants have never been made on Algerian CD's. In a case-control design, 204 Algerian with CD diagnosed for at least 5years and 201 controls were included were genotyped for single nucleotide polymorphisms (SNP) in the NOD2/CARD15 gene R702W (SNP8, rs2066844), G908R (SNP12, rs2066845) and IL23R R381Q (rs11209026) gene variants were determined using the TaqMan SNP genotyping assays. NOD2/CARD15 908R was carried by 3% of the patients and none in control subjects (χ(2)=8.6, Pc=0.003, OR=13.20). NOD2/CARD15 702W was associated to CD outcome (χ(2)=17.2, Pc=0.00003, OR=12.5) and early onset of disease (group A1, χ(2)=19.3, Pc=1.10(-5), OR=14.05, PM-H=2.10(-6)). IL23R 381Q variants was more frequent in CD's patients than controls (χ(2)=8, Pc=0.005, OR=3.48), it was associated to earlier onset (group A1, χ(2)=7.1, Pc=0.007, OR=1.04, PM-H=0.002), extra-intestinal manifestations (EIM) outcome (χ(2)=10.6, Pc=0.001, OR=1.05, PM-H=0.002) and ileocolonic location (χ(2)=6.8, Pc=0.009, OR=1.05, PM-H=0.001). In this Algerian cohort, NOD2/CARD15 and IL23R variants were associated with CD's outcomes and linked to a particular clinical phenotype.
authors.affiliation: Immunology Department, Central Hospital of Army, Algiers, Algeria.
authors.forename: Y
authors.initials: Y
authors.lastname: Meddour
authors.affiliation: Immunology Department, Central Hospital of Army, Algiers, Algeria.
authors.forename: S
authors.initials: S
authors.lastname: Chaib
authors.affiliation: Gastroenterology Department, Central Hospital of Army, Algiers, Algeria.
authors.forename: A
authors.initials: A
authors.lastname: Bousseloub
authors.affiliation: Gastroenterology Department, Mustapha Bacha Hospital, Algiers, Algeria.
authors.forename: N
authors.initials: N
authors.lastname: Kaddache
authors.affiliation: Gastroenterology Department, Mustapha Bacha Hospital, Algiers, Algeria.
authors.forename: L
authors.initials: L
authors.lastname: Kecili
authors.affiliation: Gastroenterology Department, Mustapha Bacha Hospital, Algiers, Algeria.
authors.forename: L
authors.initials: L
authors.lastname: Gamar
authors.affiliation: Gastroenterology Department, Nafissa Hamoud Hospital, Algiers, Algeria.
authors.forename: M
authors.initials: M
authors.lastname: Nakkemouche
authors.affiliation: Biology Department, Béni-Messous Hospital, Algiers, Algeria; Immunogenetics and Immunopathology Research Laboratory, Algiers, Algeria. Electronic address: ourtilane@yahoo.fr.
authors.forename: R
authors.initials: R
authors.lastname: Djidjik
authors.affiliation: Immunology Department, Pasteur Institute, Algiers, Algeria; Immunogenetics and Immunopathology Research Laboratory, Algiers, Algeria.
authors.forename: M C
authors.initials: MC
authors.lastname: Abbadi
authors.affiliation: Immunology and Histocompatibility Department, CIB-HOB, AP-HP, IUH and INSERM UMRS940, Saint-Louis Hospital, Paris, France.
authors.forename: D
authors.initials: D
authors.lastname: Charron
authors.affiliation: Gastroenterology Department, Mustapha Bacha Hospital, Algiers, Algeria.
authors.forename: T E
authors.initials: TE
authors.lastname: Boucekkine
authors.affiliation: Immunology and Histocompatibility Department, CIB-HOB, AP-HP, IUH and INSERM UMRS940, Saint-Louis Hospital, Paris, France.
authors.forename: R
authors.initials: R
authors.lastname: Tamouza
mesh_terms: D000328:Adult; D000462:Algeria; D016022:Case-Control Studies; D003424:Crohn Disease / Q000235:genetics*; D014644:Genetic Variation; D006801:Humans; D009154:Mutation; D053473:Nod2 Signaling Adaptor Protein / Q000235:genetics*; D018123:Receptors, Interleukin / Q000235:genetics*
chemical_list: C515500:IL23R protein, human; C429018:NOD2 protein, human; D053473:Nod2 Signaling Adaptor Protein; D018123:Receptors, Interleukin