title: [Increased invasion ability mechanism of salivary adenoid cystic carcinoma through elevated interstitial fluid pressure in vitro].
abstract: OBJECTIVE
  Through a simulation of interstitial fluid pressure (IFP), we developed an in vitro model to explore the change law of biological characteristics of adenoid cystic carcinoma (ACC) under different IFP.
  
  
  METHODS
  A pressure cooker was refitted into a controllable pressure device. Cultured ACC-2 cells were subdivided into different groups, namely, negative control (untreated ACC-2) and experimental group (stressed for 3, 6, 12, 24 h under pressure of 7.551, 7.649, 7.747 kPa). CCK-8 and immunofluorescence of Ki67 were used to reflect proliferation ability. Transwell chamber assay was performed to observe the invasion ability of cells.
  
  
  RESULTS
  The proliferation ability was positively correlated with treatment time, and the peak value was obtained after the cells were subjected to 7.649 kPa of stress for 24 h. The invasion ability of ACC-2 cells was upregulated under stress.
  
  
  CONCLUSIONS
  We successfully developed an in vitro model of IFP and found that high IFP can stimulate cell proliferation ability and upregulate invasion ability.
authors.forename: Yi
authors.initials: Y
authors.lastname: Huang
authors.forename: Tao
authors.initials: T
authors.lastname: Yu
authors.forename: Wenchao
authors.initials: W
authors.lastname: Zhu
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.forename: Longjiang
authors.initials: L
authors.lastname: Li
mesh_terms: D003528:Carcinoma, Adenoid Cystic; D049109:Cell Proliferation; D045604:Extracellular Fluid; D006801:Humans; D066298:In Vitro Techniques; D012468:Salivary Gland Neoplasms