title: Solid-phase synthesis of P-boronated oligonucleotides by the H-boranophosphonate method.
abstract: Recently, P-boronated oligonucleotides have been attracting much attention as potential therapeutic oligonucleotides. In this study, we developed H-boranophosphonate oligonucleotide bearing a borano group and hydrogen atom on the internucleotidic phosphorus and demonstrated that this novel P-boronated oligonucleotide is a versatile precursor to various P-boronated oligonucleotides such as boranophosphate, boranophosphorothioate, and boranophosphoramidate. The method was also applicable to the synthesis of a locked nucleic acid-modified boranophosphate oligonucleotide, which exhibited a dramatically enhanced affinity to complementary oligonucleotides.
authors.affiliation: Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo , Bioscience Building 702, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8562, Japan .
authors.forename: Sho
authors.initials: S
authors.lastname: Uehara
authors.forename: Shingo
authors.initials: S
authors.lastname: Hiura
authors.forename: Renpei
authors.initials: R
authors.lastname: Higashida
authors.forename: Natsuhisa
authors.initials: N
authors.lastname: Oka
authors.forename: Takeshi
authors.initials: T
authors.lastname: Wada
mesh_terms: D001880:Boranes / Q000737:chemistry*; D015394:Molecular Structure; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D010710:Phosphates / Q000737:chemistry*; D060327:Solid-Phase Synthesis Techniques
chemical_list: D001880:Boranes; D009841:Oligonucleotides; D010710:Phosphates; C510710:boranophosphate