title: A diazirine-based photoaffinity probe for facile and efficient aptamer-protein covalent conjugation.
abstract: A photo-reactive functional labelling reagent, diazirine phosphoramidite, was designed and synthesized for easy and flexible site-specific labelling of oligonucleotides with the diazirine moiety. The new reagent allows facile photo-crosslinking of oligonucleotide with its interacting partner for a variety of applications, including tertiary structure determination, molecular interaction study and biomarker discovery.
authors.affiliation: The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, Key Laboratory for Chemical Biology of Fujian Province, State Key Laboratory of Physical Chemistry of Solid Surfaces, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, P. R. China. cyyang@xmu.edu.cn zhuzhi@xmu.edu.cn.
authors.forename: Huimin
authors.initials: H
authors.lastname: Zhang
authors.forename: Yanling
authors.initials: Y
authors.lastname: Song
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zou
authors.forename: Yun
authors.initials: Y
authors.lastname: Ge
authors.forename: Yuan
authors.initials: Y
authors.lastname: An
authors.forename: Yanli
authors.initials: Y
authors.lastname: Ma
authors.forename: Zhi
authors.initials: Z
authors.lastname: Zhu
authors.forename: Chaoyong James
authors.initials: CJ
authors.lastname: Yang
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001389:Azirines / Q000737:chemistry*; D001665:Binding Sites; D002417:Cattle; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000737:chemistry; D009943:Organophosphorus Compounds / Q000138:chemical synthesis / Q000737:chemistry; D019920:Photoaffinity Labels / Q000737:chemistry*; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism; D014466:Ultraviolet Rays
chemical_list: D052157:Aptamers, Nucleotide; D001389:Azirines; D009841:Oligonucleotides; D009943:Organophosphorus Compounds; D019920:Photoaffinity Labels; D011506:Proteins; C434331:phosphoramidite; D012710:Serum Albumin, Bovine