title: Spot or early morning sample for mycobacterial culture: which?
abstract: Drug resistance studies ideally require two sputum specimens, one spot and one early morning, for culture in Löwenstein-Jensen medium. Although the type of specimen plays a major role in the detection of acid-fast bacilli using microscopy, this type of specimen collection puts unnecessary pressure on patients. Two sputum samples, one on the spot and another in the early morning, collected from 179 TB patients in cetylpyridinium chloride and processed for culture, yielded respectively 89.9% and 87.7% culture positivity, while culture positivity increased to 93.3% when both specimens were tested.
authors.affiliation: Regional Medical Research Centre, Bhubaneswar, India.
authors.forename: D
authors.initials: D
authors.lastname: Das
authors.affiliation: Regional Medical Research Centre, Bhubaneswar, India.
authors.forename: B
authors.initials: B
authors.lastname: Dwibedi
authors.affiliation: Regional Medical Research Centre, Bhubaneswar, India.
authors.forename: S K
authors.initials: SK
authors.lastname: Kar
mesh_terms: D000995:Antitubercular Agents / Q000627:therapeutic use; D001431:Bacteriological Techniques; D002594:Cetylpyridinium / Q000737:chemistry; D003430:Cross-Sectional Studies; D006801:Humans; D008826:Microbial Sensitivity Tests; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000302:isolation & purification*; D011237:Predictive Value of Tests; D013048:Specimen Handling / Q000379:methods*; D013183:Sputum / Q000382:microbiology*; D013997:Time Factors; D018088:Tuberculosis, Multidrug-Resistant / Q000175:diagnosis / Q000188:drug therapy / Q000382:microbiology*; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis / Q000188:drug therapy / Q000382:microbiology*
chemical_list: D000995:Antitubercular Agents; D002594:Cetylpyridinium