title: A review of analytical methods for eicosanoids in brain tissue.
abstract: Eicosanoids are potent lipid mediators of inflammation and are known to play an important role in numerous pathophysiological processes. Furthermore, inflammation has been proven to be a mediator of diseases such as hypertension, atherosclerosis, Alzheimer's disease, cancer and rheumatoid arthritis. Hence, these lipid mediators have gained significant attention in recent years. This review focuses on chromatographic and mass spectrometric methods that have been used to analyze arachidonic acid and its metabolites in brain tissue. Recently published analytical methods such as LC-MS/MS and GC-MS/MS are discussed and compared in terms of limit of quantitation and sample preparation procedures, including solid phase extraction and derivatization. Analytical challenges are also highlighted.
authors.affiliation: Temple University, Department of Chemistry, 1901 North 13th Street, Philadelphia, PA 19122, United States.
authors.forename: Michael
authors.initials: M
authors.lastname: Puppolo
authors.affiliation: Temple University, Department of Chemistry, 1901 North 13th Street, Philadelphia, PA 19122, United States.
authors.forename: Deepti
authors.initials: D
authors.lastname: Varma
authors.affiliation: Temple University, Department of Chemistry, 1901 North 13th Street, Philadelphia, PA 19122, United States. Electronic address: susan.varnum@temple.edu.
authors.forename: Susan A
authors.initials: SA
authors.lastname: Jansen
mesh_terms: D000818:Animals; D001095:Arachidonic Acids / Q000032:analysis* / Q000378:metabolism; D001921:Brain / Q000378:metabolism; D001923:Brain Chemistry; D002853:Chromatography, Liquid / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D052616:Solid Phase Extraction / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D001095:Arachidonic Acids
keywords: Brain tissue; Eicosanoids; Gas chromatography–mass spectrometry; High pressure liquid chromatography–mass spectrometry; Tandem mass spectrometry; Ultra high pressure liquid chromatography–mass spectrometry