title: Routes of allergic sensitization and myeloid cell IKKβ differentially regulate antibody responses and allergic airway inflammation in male and female mice.
abstract: Gender influences the incidence and/or the severity of several diseases and evidence suggests a higher rate of allergy and asthma among women. Most experimental models of allergy use mice sensitized via the parenteral route despite the fact that the mucosal tissues of the gastrointestinal and respiratory tracts are major sites of allergic sensitization and/or allergic responses. We analyzed allergen-specific Ab responses in mice sensitized either by gavage or intraperitoneal injection of ovalbumin together with cholera toxin as adjuvant, as well as allergic inflammation and lung functions following subsequent nasal challenge with the allergen. Female mice sensitized intraperitoneally exhibited higher levels of serum IgE than their male counterparts. After nasal allergen challenge, these female mice expressed higher Th2 responses and associated inflammation in the lung than males. On the other hand, male and female mice sensitized orally developed the same levels of allergen-specific Ab responses and similar levels of lung inflammation after allergen challenge. Interestingly, the difference in allergen-specific Ab responses between male and female mice sensitized by the intraperitoneal route was abolished in IKKβΔMye mice, which lack IKKβ in myeloid cells. In summary, the oral or systemic route of allergic sensitization and IKKβ signaling in myeloid cells regulate how the gender influences allergen-specific responses and lung allergic inflammation.
authors.affiliation: Department of Veterinary Biosciences, The Ohio State University, Columbus, Ohio, United States of America; Laboratory of Human Nutrition, AgroParisTech, Paris, France.
authors.forename: Astrid
authors.initials: A
authors.lastname: Bonnegarde-Bernard
authors.affiliation: Department of Veterinary Biosciences, The Ohio State University, Columbus, Ohio, United States of America.
authors.forename: Junbae
authors.initials: J
authors.lastname: Jee
authors.affiliation: Department of Veterinary Biosciences, The Ohio State University, Columbus, Ohio, United States of America.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Fial
authors.affiliation: Department of Veterinary Biosciences, The Ohio State University, Columbus, Ohio, United States of America.
authors.forename: Haley
authors.initials: H
authors.lastname: Steiner
authors.affiliation: Department of Veterinary Biosciences, The Ohio State University, Columbus, Ohio, United States of America.
authors.forename: Stephanie
authors.initials: S
authors.lastname: DiBartola
authors.affiliation: Department of Veterinary Biosciences, The Ohio State University, Columbus, Ohio, United States of America.
authors.forename: Ian C
authors.initials: IC
authors.lastname: Davis
authors.affiliation: Department of Veterinary Biosciences, The Ohio State University, Columbus, Ohio, United States of America.
authors.forename: Estelle
authors.initials: E
authors.lastname: Cormet-Boyaka
authors.affiliation: Laboratory of Human Nutrition, AgroParisTech, Paris, France.
authors.forename: Daniel
authors.initials: D
authors.lastname: Tomé
authors.affiliation: Department of Veterinary Biosciences, The Ohio State University, Columbus, Ohio, United States of America; Department of Internal Medicine, The Ohio State University, Columbus, Ohio, United States of America.
authors.forename: Prosper N
authors.initials: PN
authors.lastname: Boyaka
mesh_terms: D000284:Administration, Oral; D000485:Allergens / Q000008:administration & dosage / Q000276:immunology*; D000818:Animals; D000917:Antibody Formation / Q000276:immunology*; D015153:Blotting, Western; D002772:Cholera Toxin / Q000276:immunology; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D004333:Drug Administration Routes; D005260:Female; D051550:I-kappa B Kinase / Q000502:physiology*; D007249:Inflammation / Q000276:immunology* / Q000473:pathology; D007274:Injections, Intraperitoneal; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D022423:Myeloid Cells / Q000276:immunology* / Q000473:pathology; D010047:Ovalbumin / Q000276:immunology; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D012137:Respiratory System / Q000276:immunology* / Q000473:pathology; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000485:Allergens; D016207:Cytokines; D012333:RNA, Messenger; D010047:Ovalbumin; D002772:Cholera Toxin; D051550:I-kappa B Kinase