title: In vitro investigation of toxicological interactions between the fusariotoxins deoxynivalenol and zearalenone.
abstract: It is expected that humans are exposed to combined mycotoxins, which occur simultaneously in the food items, than to individual compounds and that can increase their potential toxicity. Considering this coincident production, deoxynivalenol (DON) and zearalenone (ZEN) as they are produced by several Fusarium species, can interfere at a cellular level. Therefore, these two toxins were chosen to study their interactive effects on human colon carcinoma cells (HCT116), using the endpoints including cell viability, cell cycle analysis, mitochondrial transmembrane potential (ΔΨm) determination and permeability transition pore (PTP) opening. Our results showed that DON and ZEN caused a marked decrease of cell viability in a dose-dependent manner, mediated by an activation of the mitochondrial apoptotic process; characterized by PTP opening and the loss of ΔΨm. Nevertheless, combined DON and ZEN reduced all the toxicities observed with the mycotoxins separately. Therefore, the combination of the two mycotoxins appears as a sub-additive response.
authors.affiliation: Laboratory for Research on Biologically Compatible Compounds, Faculty of Dentistry, Rue Avicenne, 5019 Monastir, Tunisia; Laboratory of Plant Protection, The National Institute for Agricultural Research, INRA Tunisia, Rue Hedi Karray, 2049 Ariana, Tunisia. Electronic address: bensassifatma83@yahoo.fr.
authors.forename: Fatma
authors.initials: F
authors.lastname: Bensassi
authors.affiliation: INSERM U769, LabEx LERMIT, Signalisation et Physiopathologie Cardiaque, Faculté de Pharmacie, Châtenay-Malabry, France.
authors.forename: Cindy
authors.initials: C
authors.lastname: Gallerne
authors.affiliation: INSERM U769, LabEx LERMIT, Signalisation et Physiopathologie Cardiaque, Faculté de Pharmacie, Châtenay-Malabry, France.
authors.forename: Ossama
authors.initials: O
authors.lastname: Sharaf el Dein
authors.affiliation: Laboratory of Plant Protection, The National Institute for Agricultural Research, INRA Tunisia, Rue Hedi Karray, 2049 Ariana, Tunisia.
authors.forename: Mohamed Rabeh
authors.initials: MR
authors.lastname: Hajlaoui
authors.affiliation: INSERM U769, LabEx LERMIT, Signalisation et Physiopathologie Cardiaque, Faculté de Pharmacie, Châtenay-Malabry, France; Université de Versailles St. Quentin, Versailles 78035, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Lemaire
authors.affiliation: Laboratory for Research on Biologically Compatible Compounds, Faculty of Dentistry, Rue Avicenne, 5019 Monastir, Tunisia.
authors.forename: Hassen
authors.initials: H
authors.lastname: Bacha
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D045325:HCT116 Cells; D006801:Humans; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D033681:Mitochondrial Membrane Transport Proteins / Q000187:drug effects; D000083162:Mitochondrial Permeability Transition Pore; D009183:Mycotoxins / Q000633:toxicity*; D014255:Trichothecenes / Q000633:toxicity*; D015025:Zearalenone / Q000633:toxicity*
chemical_list: D033681:Mitochondrial Membrane Transport Proteins; D000083162:Mitochondrial Permeability Transition Pore; D009183:Mycotoxins; D014255:Trichothecenes; D015025:Zearalenone; C007262:deoxynivalenol
keywords: Apoptosis; Combination; Deoxynivalenol; Mitochondria; PTP; Zearalenone