title: Replication of migraine GWAS susceptibility loci in Chinese Han population.
abstract: BACKGROUND
  Recent genome-wide association studies (GWAS) have identified 3 genetic variants that are strongly associated with migraine in Europeans. The effect of these risk variants in other populations is unknown. To further replicate the GWAS findings, we investigated the 3 variants rs2651899 (1p36.32, PRDM16), rs10166942 (2q37.1, TRPM8), and rs11172113 (12q13.3, LRP1) for their association with migraine in the Chinese Han population.
  
  
  METHODS
  We performed a case-control association study. Genomic DNA was collected from 608 unrelated individuals, including 304 migraineurs (41 migraine with aura and 263 migraine without aura) and 304 healthy controls. Genotyping of single nucleotide polymorphisms (SNPs) was performed by ligase detection reaction method.
  
  
  RESULTS
  We identified the minor allele of rs2651899 located in PRDM16 to be associated with migraine (P = .005, odds ratio = 1.382, 95% confidence interval = 1.100-1.736), the association remain significant after Bonferroni correction. For the other 2 SNPs (rs10166942 and rs11172113), no statistically significant differences were observed in the allele/genotype frequencies between cases and controls. None of the 3 SNP was associated with specific migraine features.
  
  
  CONCLUSIONS
  Our study confirmed the association of PRDM16 to migraine susceptibility in the Chinese Han population. The results also indicated that replication studies of previous GWAS findings across populations is of importance to validate these associations and to gain a better understanding of migraine susceptibility of potential genetic heterogeneity between populations. Further work is necessary to understand the functional mechanisms underlying these variants identified by GWAS.
authors.affiliation: Hospice, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Fan
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Wen
authors.initials: W
authors.lastname: Fan
authors.forename: Lixue
authors.initials: L
authors.lastname: Chen
authors.forename: Bei
authors.initials: B
authors.lastname: Gui
authors.forename: Ge
authors.initials: G
authors.lastname: Tan
authors.forename: Jiying
authors.initials: J
authors.lastname: Zhou
mesh_terms: D000328:Adult; D044466:Asian People / Q000235:genetics*; D016022:Case-Control Studies; D004268:DNA-Binding Proteins / Q000235:genetics*; D005260:Female; D056426:Genetic Loci; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D055106:Genome-Wide Association Study; D005838:Genotype; D006801:Humans; D026503:Low Density Lipoprotein Receptor-Related Protein-1 / Q000235:genetics*; D008297:Male; D008881:Migraine Disorders / Q000235:genetics*; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D050053:TRPM Cation Channels / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; C475476:LRP1 protein, human; D026503:Low Density Lipoprotein Receptor-Related Protein-1; C416371:PRDM16 protein, human; D050053:TRPM Cation Channels; C425966:TRPM8 protein, human; D014157:Transcription Factors
keywords: genome-wide association study; migraine susceptibility; single nucleotide polymorphism