title: Transphosphorylation of EGFR at Y845 plays an important role in its autophosphorylation and kinase activity.
abstract: Epidermal growth factor receptor (EGFR) plays a critical role in various types of human cancer such as lung, breast, brain and colon cancer. However, how EGFR activation is initiated and what type of interaction is linked to its transphosphorylation and autophosphorylation as well as the biological consequences in case of interruption and blockade of these two types of EGFR phosphorylation, remain elusive. In the present study, we provided evidence that EGFR transphosphorylation at Y845 indeed plays an important role in its autophosphorylation and kinase activity. Our results suggest that the modulation of EGFR transphosphorylation may influence its activity and function, indicating a potential target mechanism for treating EGFR-associated diseases and various types of cancer.
authors.affiliation: The Cyrus Tang Hematology Center, Soochow University, Suzhou, Jiangsu 215123, P.R. China.
authors.forename: Hui
authors.initials: H
authors.lastname: Song
authors.affiliation: The Cyrus Tang Hematology Center, Soochow University, Suzhou, Jiangsu 215123, P.R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Huang
authors.affiliation: Xi'an Jiaotong University Suzhou Academy, Suzhou, Jiangsu 215123, P.R. China.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Xi'an Jiaotong University Suzhou Academy, Suzhou, Jiangsu 215123, P.R. China.
authors.forename: Xiuwei
authors.initials: X
authors.lastname: Wang
authors.affiliation: Xi'an Jiaotong University Suzhou Academy, Suzhou, Jiangsu 215123, P.R. China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Song
authors.affiliation: The Cyrus Tang Hematology Center, Soochow University, Suzhou, Jiangsu 215123, P.R. China.
authors.forename: Lin
authors.initials: L
authors.lastname: Yang
mesh_terms: D019943:Amino Acid Substitution / Q000235:genetics*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D004261:DNA Replication / Q000235:genetics; D004815:Epidermal Growth Factor / Q000378:metabolism; D066246:ErbB Receptors / Q000235:genetics* / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D061986:MCF-7 Cells; D009154:Mutation; D009369:Neoplasms / Q000473:pathology; D010766:Phosphorylation; D010770:Phosphotransferases / Q000378:metabolism
chemical_list: D004815:Epidermal Growth Factor; D010770:Phosphotransferases; C512478:EGFR protein, human; D066246:ErbB Receptors