title: TCA precipitation.
abstract: Trichloroacetic acid (TCA) precipitation of proteins is commonly used to concentrate protein samples or remove contaminants, including salts and detergents, prior to downstream applications such as SDS-PAGE or 2D-gels. TCA precipitation denatures the protein, so it should not be used if the protein must remain in its folded state (e.g., if you want to measure a biochemical activity of the protein).
authors.affiliation: Department of Molecular Biology and Genetics, Johns Hopkins School of Medicine, Baltimore, MD, USA. Electronic address: laurakoontz@gmail.com.
authors.forename: Laura
authors.initials: L
authors.lastname: Koontz
mesh_terms: D001671:Biochemistry / Q000379:methods*; D011232:Chemical Precipitation; D011489:Protein Denaturation; D011506:Proteins / Q000737:chemistry*; D014238:Trichloroacetic Acid / Q000737:chemistry*
chemical_list: D011506:Proteins; D014238:Trichloroacetic Acid
keywords: Bromophenol blue; Deoxycholate-trichloroacetic acid precipitation; Dithiothreitol (DTT); Glycerol; Sodium dodecyl sulfate (SDS); Sodium hydroxide (NaOH); Trichloroacetic acid (TCA); Tris base