title: Approaches to engineer stability of beetle luciferases.
abstract: Luciferase enzymes from fireflies and other beetles have many important applications in molecular biology, biotechnology, analytical chemistry and several other areas. Many novel beetle luciferases with promising properties have been reported in the recent years. However, actual and potential applications of wild-type beetle luciferases are often limited by insufficient stability or decrease in activity of the enzyme at the conditions of a particular assay. Various examples of genetic engineering of the enhanced beetle luciferases have been reported that successfully solve or alleviate many of these limitations. This mini-review summarizes the recent advances in development of mutant luciferases with improved stability and activity characteristics. It discusses the common limitations of wild-type luciferases in different applications and presents the efficient approaches that can be used to address these problems.
authors.affiliation: Department of Chemical Enzymology, Faculty of Chemistry, Lomonosov Moscow State University, Moscow, 119991, Russia.
authors.forename: Mikhail I
authors.initials: MI
authors.lastname: Koksharov
authors.affiliation: Department of Chemical Enzymology, Faculty of Chemistry, Lomonosov Moscow State University, Moscow, 119991, Russia.
authors.forename: Natalia N
authors.initials: NN
authors.lastname: Ugarova
keywords: Bioluminescence; Firefly luciferase; directed evolution; protein engineering; thermal stability