title: Herpes simplex virus mutant generation and dual-detection methods for gaining insight into latent/lytic cycles in vivo.
abstract: Two important components to a useful strategy to examine viral gene regulation in vivo are (1) a highly efficient protocol to generate viral mutants that limits undesired mutation and retains full replication competency in vivo and (2) an efficient system to detect and quantify viral promoter activity in rare cells in vivo. Our strategy and protocols for generating, characterizing, and employing HSV viral promoter/reporter mutants in vivo are provided in this two-part chapter.
authors.affiliation: Division of Infectious Diseases, Department of Pediatrics, Cincinnati Children's Hospital Medical Center, 3333 Burnet Ave., MLC 7017, Cincinnati, OH, 45229-3039, USA, nancy.sawtell@cchmc.org.
authors.forename: Nancy M
authors.initials: NM
authors.lastname: Sawtell
authors.forename: Richard L
authors.initials: RL
authors.lastname: Thompson
mesh_terms: D015967:Gene Expression Regulation, Viral; D006801:Humans; D008967:Molecular Biology / Q000379:methods*; D009154:Mutation; D011401:Promoter Regions, Genetic; D018139:Simplexvirus / Q000235:genetics* / Q000254:growth & development; D017735:Virus Latency; D018406:Virus Physiological Phenomena; D014779:Virus Replication / Q000235:genetics*