title: Coupling antibody to cyanogen bromide-activated sepharose.
abstract: Antibodies will be immobilized on a cyanogen bromide-activated Sepharose for subsequent use in pull-down assays or immunoaffinity purification.
authors.affiliation: Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
authors.forename: Jennifer M
authors.initials: JM
authors.lastname: Kavran
authors.affiliation: Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA. Electronic address: dleahy@jhmi.edu.
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Leahy
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry*; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D002846:Chromatography, Affinity / Q000295:instrumentation; D003488:Cyanogen Bromide / Q000737:chemistry*; D012117:Resins, Synthetic / Q000737:chemistry; D012685:Sepharose / Q000737:chemistry*
chemical_list: D055910:Antibodies, Immobilized; D000911:Antibodies, Monoclonal; D012117:Resins, Synthetic; D012685:Sepharose; D003488:Cyanogen Bromide
keywords: Antibody and resin preparation; Antigen–antibody reactions; Ascitic fluid; Coupling antibody to resin; Cyanogen bromide (CNBr)-activated Sepharose; Hybridoma; Resin