title: Rapid detection of microbes in the dialysis solution by the microcolony fluorescence staining method (Millflex quantum).
abstract: A chemiluminescence system, Milliflex Quantum (MFQ), to detect microcolonies, has been used in the pharmaceutical field. In this study, we investigated aquatic bacteria in hemodialysis solutions sampled from bioburden areas in 4 dialysis faculties. Using MFQ, microcolonies could be detected after a short incubation period. The colony count detected with MFQ after a 48-hour incubation was 92% ± 39%, compared to that after the conventionally used 7-14-day incubation period; in addition, the results also showed a linear correlation. Moreover, MFQ-based analysis allowed the visualization of damaged cells and of the high density due to the excessive amount of bacteria. These results suggested that MFQ had adequate sensitivity to detect microbacteria in dialysis solutions, and it was useful for validating the conditions of conventional culture methods.
authors.affiliation: Quality Control Section.
authors.forename: Eiichi
authors.initials: E
authors.lastname: Osono
authors.forename: Eiko
authors.initials: E
authors.lastname: Kobayashi
authors.forename: Yuki
authors.initials: Y
authors.lastname: Inoue
authors.forename: Kazumi
authors.initials: K
authors.lastname: Honda
authors.forename: Takuya
authors.initials: T
authors.lastname: Kumagai
authors.forename: Hideki
authors.initials: H
authors.lastname: Negishi
authors.forename: Kentaro
authors.initials: K
authors.lastname: Okamatsu
authors.forename: Kyoko
authors.initials: K
authors.lastname: Ichimura
authors.forename: Chisako
authors.initials: C
authors.lastname: Kamano
authors.forename: Fumi
authors.initials: F
authors.lastname: Suzuki
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Norose
authors.forename: Megumi
authors.initials: M
authors.lastname: Takahashi
authors.forename: Shun
authors.initials: S
authors.lastname: Takaku
authors.forename: Noriaki
authors.initials: N
authors.lastname: Fujioka
authors.forename: Naoaki
authors.initials: N
authors.lastname: Hayama
authors.forename: Hideaki
authors.initials: H
authors.lastname: Takizawa
mesh_terms: D001419:Bacteria / Q000737:chemistry / Q000254:growth & development / Q000302:isolation & purification*; D015314:Dialysis Solutions / Q000737:chemistry*; D004340:Drug Contamination; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods*; D013194:Staining and Labeling
chemical_list: D015314:Dialysis Solutions