title: Extraction and in vitro antioxidant activity of exopolysaccharide by Pleurotus eryngii SI-02.
abstract: The extraction parameters for Pleurotus eryngii SI-02 exopolysaccharide (EPS) produced during submerged culture were optimized using response surface methodology (RSM). The optimum conditions for EPS extraction were predicted to be, precipitation time 20.24 h, ethanol concentration 89.62% and pH 8.17, and EPS production was estimated at 7.27 g/L. The actual yield of EPS under these conditions was 7.21 g/L. The in vitro antioxidant results of the EPS showed that the inhibition effects of EPS at a dosage of 400 mg/L on hydroxyl, superoxide anion and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals were 59.63 ± 3.72%, 38.69 ± 2.59%, and 66.36 ± 4.42%, respectively, which were 12.74 ± 1.03%, 8.01 ± 0.56%, and 12.19 ± 1.05% higher than that of butylated hydroxytoluene (BHT), respectively. The reducing power of EPS of P. eryngii SI-02 was 0.98 ± 0.05, 60.66 ± 5.14% higher than that of BHT. The results provide a reference for large-scale production of EPS by P. eryngii SI-02 in industrial fermentation and the EPS can be used as a potential antioxidant which enhances adaptive immune responses.
authors.affiliation: College of Life Science, Shandong Agricultural University, Taian, Shandong 271018, PR China.
authors.forename: Xinyi
authors.initials: X
authors.lastname: Sun
authors.affiliation: College of Life Science, Shandong Agricultural University, Taian, Shandong 271018, PR China.
authors.forename: Long
authors.initials: L
authors.lastname: Hao
authors.affiliation: The Central Hospital of Taian, Shandong, PR China.
authors.forename: Hua
authors.initials: H
authors.lastname: Ma
authors.affiliation: College of Mathematics and Applied Mathematics, Fudan University, Shanghai, PR China.
authors.forename: Tong
authors.initials: T
authors.lastname: Li
authors.affiliation: College of Life Science, Shandong Agricultural University, Taian, Shandong 271018, PR China.
authors.forename: Lan
authors.initials: L
authors.lastname: Zheng
authors.affiliation: College of Life Science, Shandong Agricultural University, Taian, Shandong 271018, PR China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Ma
authors.affiliation: College of Life Science, Shandong Agricultural University, Taian, Shandong 271018, PR China.
authors.forename: Guoyin
authors.initials: G
authors.lastname: Zhai
authors.affiliation: College of Life Science, Shandong Agricultural University, Taian, Shandong 271018, PR China.
authors.forename: Liqin
authors.initials: L
authors.lastname: Wang
authors.affiliation: College of Life Science, Shandong Agricultural University, Taian, Shandong 271018, PR China.
authors.forename: Shanglong
authors.initials: S
authors.lastname: Gao
authors.affiliation: College of Life Science, Shandong Agricultural University, Taian, Shandong 271018, PR China.
authors.forename: Xiaonan
authors.initials: X
authors.lastname: Liu
authors.affiliation: The Second High of Taian, Shandong, PR China.
authors.forename: Mengshi
authors.initials: M
authors.lastname: Jia
authors.affiliation: College of Life Science, Shandong Agricultural University, Taian, Shandong 271018, PR China.
authors.forename: Le
authors.initials: L
authors.lastname: Jia
mesh_terms: D000975:Antioxidants / Q000302:isolation & purification* / Q000378:metabolism*; D003627:Data Interpretation, Statistical; D000431:Ethanol / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D020076:Pleurotus / Q000737:chemistry*; D011134:Polysaccharides / Q000302:isolation & purification* / Q000378:metabolism*; D012997:Solvents / Q000378:metabolism; D013997:Time Factors
chemical_list: D000975:Antioxidants; D011134:Polysaccharides; D012997:Solvents; D000431:Ethanol
keywords: Pleurotus eryngii SI-02; antioxidant activity; exopolysaccharide; extraction; in vitro