title: RBP4 gene variants are associated with insulin resistance in women with previous gestational diabetes.
abstract: OBJECTIVE
  This study aimed to examine possible genetic effects of some retinol binding protein-4 (RBP4) single nucleotide polymorphisms (SNPs) on the risk of gestational diabetes mellitus (GDM). In addition, the SNPs were examined for their possible association with insulin resistance at 6 weeks after delivery.
  
  
  METHODS
  This was a prospective study of 100 women with GDM and 100 participants with normal gestation who were evaluated at gestational week 30 and 6 weeks postpartum. Three SNPs of RBP4 (rs3758539, rs116736522, and rs34571439) were genotyped using TaqMan assay. The genotype distributions between GDM patients and normal controls were analyzed using logistic regression models. In addition, differences in clinical characteristics among subjects grouped by genotype were assessed using the analysis of covariance test.
  
  
  RESULTS
  The frequencies of the rare alleles were not significantly different between GDM patients and controls. However, we identified two variants rs3758539 and rs34571439 associated with insulin levels and insulin resistance in women with previous GDM.
  
  
  CONCLUSIONS
  Noncoding SNPs of the RBP4 gene are not associated with GDM, but two SNPs showed associations with insulin resistance and insulin levels in women with prior GDM. Additional studies with increased sample size will be necessary in other GDM cohorts.
authors.affiliation: Endocrine Research Unit, National Medical Center, Mexican Social Security Institute, 06720 Mexico City, MX, Mexico.
authors.forename: Renata
authors.identifier: 0000-0001-5167-6156
authors.initials: R
authors.lastname: Saucedo
authors.affiliation: Endocrine Research Unit, National Medical Center, Mexican Social Security Institute, 06720 Mexico City, MX, Mexico.
authors.forename: Arturo
authors.identifier: 0000-0003-3606-0390
authors.initials: A
authors.lastname: Zarate
authors.affiliation: Endocrine Research Unit, National Medical Center, Mexican Social Security Institute, 06720 Mexico City, MX, Mexico.
authors.forename: Lourdes
authors.identifier: 0000-0002-2991-0433
authors.initials: L
authors.lastname: Basurto
authors.affiliation: Endocrine Research Unit, National Medical Center, Mexican Social Security Institute, 06720 Mexico City, MX, Mexico.
authors.forename: Marcelino
authors.initials: M
authors.lastname: Hernandez
authors.affiliation: Hospital of Gynecology and Obstetrics, Medical Center La Raza, Mexican Social Security Institute, 02990 Mexico City, MX, Mexico.
authors.forename: Edgardo
authors.initials: E
authors.lastname: Puello
authors.affiliation: Department of Genomic Medicine and Environmental Toxicology, Instituto de Investigaciones Biomedicas, Autonomous National University of Mexico, 04510 Mexico City, MX, Mexico.
authors.forename: Patricia
authors.identifier: 0000-0002-0395-1122
authors.initials: P
authors.lastname: Mendoza-Lorenzo
authors.affiliation: Department of Genomic Medicine and Environmental Toxicology, Instituto de Investigaciones Biomedicas, Autonomous National University of Mexico, 04510 Mexico City, MX, Mexico.
authors.forename: Patricia
authors.initials: P
authors.lastname: Ostrosky-Wegman
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D016640:Diabetes, Gestational / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006720:Homozygote; D006801:Humans; D007333:Insulin Resistance / Q000235:genetics*; D015810:Linkage Disequilibrium; D020641:Polymorphism, Single Nucleotide; D011247:Pregnancy; D011446:Prospective Studies; D054839:Retinol-Binding Proteins, Plasma / Q000235:genetics*; D017422:Sequence Analysis, DNA; D055815:Young Adult
chemical_list: C101326:RBP4 protein, human; D054839:Retinol-Binding Proteins, Plasma