title: Development and validation of S-allyl-L-cysteine in rat plasma using a mixed-mode reversed-phase and cation-exchange LC-ESI-MS/MS method: application to pharmacokinetic studies.
abstract: S-Allyl-L-cysteine (SAC), the most abundant organosulfur compound derived from garlic, has multifunctional biological activities that occur via different mechanisms. A sensitive, rapid and simple LC-ESI-MS/MS method using a mixed-mode reversed-phase and cation-exchange column containing C18 silica particles and sulfonic acid cation-exchange particles has been developed and validated for the analysis of SAC in rat plasma. The mobile phase was optimized at 2 mM ammonium acetate buffer (pH = 3.5) and acetonitrile (75:25, v/v). The assay utilized 0.6% acetic acid in methanol to achieve simple and rapid deproteinization. Quantification was conducted using multiple reaction monitoring (MRM) of the transitions of m/z 162.0 → 145.0 for SAC. The standard curve for SAC was linear (r(2) ≥ 0.999) over a range from 5 to 2,500 ng/mL. The intra- and interday precision (relative standard deviation) of the method was not >6.0% at three quality control levels. The limit of quantification (LOQ) was 5.0 ng/mL. After being fully validated, the method was successfully applied to the pharmacokinetic monitoring of SAC in rat plasma.
authors.affiliation: Korea Food Research Institute, Sungnam-si 463-746, South Korea shlee@kfri.re.kr.
authors.forename: Sanghee
authors.initials: S
authors.lastname: Lee
authors.affiliation: Korea Food Research Institute, Sungnam-si 463-746, South Korea.
authors.forename: Nae In
authors.initials: NI
authors.lastname: Chang
authors.affiliation: Korea Food Research Institute, Sungnam-si 463-746, South Korea.
authors.forename: Miyoung
authors.initials: M
authors.lastname: Yoo
authors.affiliation: Department of Anatomy, College of Veterinary Medicine Kangwon National University, Chuncheon 200-701, South Korea.
authors.forename: Jung Hoon
authors.initials: JH
authors.lastname: Choi
authors.affiliation: Korea Food Research Institute, Sungnam-si 463-746, South Korea.
authors.forename: Dongbin
authors.initials: D
authors.lastname: Shin
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003545:Cysteine / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics; D051381:Rats; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: C065299:S-allylcysteine; D003545:Cysteine