title: Effect of pulse electric fields (PEF) on accumulation of magnesium and zinc ions in Saccharomyces cerevisiae cells.
abstract: Cultures of Saccharomyces cerevisiae were treated with PEF to improve simultaneous accumulation of magnesium and zinc ions in the biomass. The results showed that the ions concentration in the medium and their mutual interactions affect accumulation in cells. Increasing the concentration of one ion in the medium reduced the accumulation of the second one, in the control as well as in the cells treated with PEF. Under optimized conditions, that is, on 15 min exposure of the 20 h grown culture to PEF of 5.0 kV/cm and 20 μs pulse width, accumulation of magnesium and zinc in yeast biomass reached maximum levels of 2.85 and 11.41 mg/gd.m., respectively, To summarize, optimization of ion pair concentration and PEF parameters caused a 1.5 or 2-fold increase of magnesium and zinc accumulation, respectively, in S. cerevisiae.
authors.affiliation: Department of Analysis and Evaluation of Food Quality, Faculty of Food Science and Biotechnology, University of Life Sciences, Skromna Street 8, 20-704 Lublin, Poland. Electronic address: urszula.pankiewicz@up.lublin.pl.
authors.forename: Urszula
authors.initials: U
authors.lastname: Pankiewicz
authors.affiliation: Department of Analysis and Evaluation of Food Quality, Faculty of Food Science and Biotechnology, University of Life Sciences, Skromna Street 8, 20-704 Lublin, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Sujka
authors.affiliation: Department of Analysis and Evaluation of Food Quality, Faculty of Food Science and Biotechnology, University of Life Sciences, Skromna Street 8, 20-704 Lublin, Poland.
authors.forename: Marzena
authors.initials: M
authors.lastname: Włodarczyk-Stasiak
authors.affiliation: Department of Analysis and Evaluation of Food Quality, Faculty of Food Science and Biotechnology, University of Life Sciences, Skromna Street 8, 20-704 Lublin, Poland.
authors.forename: Artur
authors.initials: A
authors.lastname: Mazurek
authors.affiliation: Department of Analysis and Evaluation of Food Quality, Faculty of Food Science and Biotechnology, University of Life Sciences, Skromna Street 8, 20-704 Lublin, Poland.
authors.forename: Jerzy
authors.initials: J
authors.lastname: Jamroz
mesh_terms: D003470:Culture Media; D004574:Electromagnetic Fields; D007477:Ions; D008274:Magnesium / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000166:cytology*; D015032:Zinc / Q000737:chemistry*
chemical_list: D003470:Culture Media; D007477:Ions; D008274:Magnesium; D015032:Zinc
keywords: Biomass; Magnesium; Pulsed electric fields; Saccharomyces cerevisiae; TEM–EDS; Zinc