title: Maternal age effect on mouse oocytes: new biological insight from proteomic analysis.
abstract: The long-standing view of 'immortal germline vs mortal soma' poses a fundamental question in biology concerning how oocytes age in molecular terms. A mainstream hypothesis is that maternal ageing of oocytes has its roots in gene transcription. Investigating the proteins resulting from mRNA translation would reveal how far the levels of functionally available proteins correlate with mRNAs and would offer novel insights into the changes oocytes undergo during maternal ageing. Gene ontology (GO) semantic analysis revealed a high similarity of the detected proteome (2324 proteins) to the transcriptome (22 334 mRNAs), although not all proteins had a cognate mRNA. Concerning their dynamics, fourfold changes of abundance were more frequent in the proteome (3%) than the transcriptome (0.05%), with no correlation. Whereas proteins associated with the nucleus (e.g. structural maintenance of chromosomes and spindle-assembly checkpoints) were largely represented among those that change in oocytes during maternal ageing; proteins associated with oxidative stress/damage (e.g. superoxide dismutase) were infrequent. These quantitative alterations are either impoverishing or enriching. Using GO analysis, these alterations do not relate in any simple way to the classic signature of ageing known from somatic tissues. Given the lack of correlation, we conclude that proteome analysis of mouse oocytes may not be surrogated with transcriptome analysis. Furthermore, we conclude that the classic features of ageing may not be transposed from somatic tissues to oocytes in a one-to-one fashion. Overall, there is more to the maternal ageing of oocytes than mere cellular deterioration exemplified by the notorious increase of meiotic aneuploidy.
authors.affiliation: Max Planck Institute for Molecular BiomedicineRöntgenstraße 20, D-48149 Münster, GermanyDZNEGerman Centre for Neurodegenerative Disorders, Gehlsheimer Straße 20, D-18147 Rostock, GermanyInstitute for Biostatistics and Informatics in Medicine and Ageing ResearchRostock University Medical Center, Ernst Heydemann-Str. 8, D-18057 Rostock, GermanyArrows Biomedical GmbHGievenbecker Weg 11, D-48149 Münster, GermanyMax Planck Institute for Molecular BiomedicineBioanalytical Mass Spectrometry Facility, Röntgenstraße 20, D-48149 Münster, Germany.
authors.forename: Caroline
authors.initials: C
authors.lastname: Schwarzer
authors.affiliation: Max Planck Institute for Molecular BiomedicineRöntgenstraße 20, D-48149 Münster, GermanyDZNEGerman Centre for Neurodegenerative Disorders, Gehlsheimer Straße 20, D-18147 Rostock, GermanyInstitute for Biostatistics and Informatics in Medicine and Ageing ResearchRostock University Medical Center, Ernst Heydemann-Str. 8, D-18057 Rostock, GermanyArrows Biomedical GmbHGievenbecker Weg 11, D-48149 Münster, GermanyMax Planck Institute for Molecular BiomedicineBioanalytical Mass Spectrometry Facility, Röntgenstraße 20, D-48149 Münster, GermanyMax Planck Institute for Molecular BiomedicineRöntgenstraße 20, D-48149 Münster, GermanyDZNEGerman Centre for Neurodegenerative Disorders, Gehlsheimer Straße 20, D-18147 Rostock, GermanyInstitute for Biostatistics and Informatics in Medicine and Ageing ResearchRostock University Medical Center, Ernst Heydemann-Str. 8, D-18057 Rostock, GermanyArrows Biomedical GmbHGievenbecker Weg 11, D-48149 Münster, GermanyMax Planck Institute for Molecular BiomedicineBioanalytical Mass Spectrometry Facility, Röntgenstraße 20, D-48149 Münster, Germany.
authors.forename: Marcin
authors.initials: M
authors.lastname: Siatkowski
authors.affiliation: Max Planck Institute for Molecular BiomedicineRöntgenstraße 20, D-48149 Münster, GermanyDZNEGerman Centre for Neurodegenerative Disorders, Gehlsheimer Straße 20, D-18147 Rostock, GermanyInstitute for Biostatistics and Informatics in Medicine and Ageing ResearchRostock University Medical Center, Ernst Heydemann-Str. 8, D-18057 Rostock, GermanyArrows Biomedical GmbHGievenbecker Weg 11, D-48149 Münster, GermanyMax Planck Institute for Molecular BiomedicineBioanalytical Mass Spectrometry Facility, Röntgenstraße 20, D-48149 Münster, Germany.
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Pfeiffer
authors.affiliation: Max Planck Institute for Molecular BiomedicineRöntgenstraße 20, D-48149 Münster, GermanyDZNEGerman Centre for Neurodegenerative Disorders, Gehlsheimer Straße 20, D-18147 Rostock, GermanyInstitute for Biostatistics and Informatics in Medicine and Ageing ResearchRostock University Medical Center, Ernst Heydemann-Str. 8, D-18057 Rostock, GermanyArrows Biomedical GmbHGievenbecker Weg 11, D-48149 Münster, GermanyMax Planck Institute for Molecular BiomedicineBioanalytical Mass Spectrometry Facility, Röntgenstraße 20, D-48149 Münster, Germany.
authors.forename: Nicole
authors.initials: N
authors.lastname: Baeumer
authors.affiliation: Max Planck Institute for Molecular BiomedicineRöntgenstraße 20, D-48149 Münster, GermanyDZNEGerman Centre for Neurodegenerative Disorders, Gehlsheimer Straße 20, D-18147 Rostock, GermanyInstitute for Biostatistics and Informatics in Medicine and Ageing ResearchRostock University Medical Center, Ernst Heydemann-Str. 8, D-18057 Rostock, GermanyArrows Biomedical GmbHGievenbecker Weg 11, D-48149 Münster, GermanyMax Planck Institute for Molecular BiomedicineBioanalytical Mass Spectrometry Facility, Röntgenstraße 20, D-48149 Münster, Germany.
authors.forename: Hannes C A
authors.initials: HC
authors.lastname: Drexler
authors.affiliation: Max Planck Institute for Molecular BiomedicineRöntgenstraße 20, D-48149 Münster, GermanyDZNEGerman Centre for Neurodegenerative Disorders, Gehlsheimer Straße 20, D-18147 Rostock, GermanyInstitute for Biostatistics and Informatics in Medicine and Ageing ResearchRostock University Medical Center, Ernst Heydemann-Str. 8, D-18057 Rostock, GermanyArrows Biomedical GmbHGievenbecker Weg 11, D-48149 Münster, GermanyMax Planck Institute for Molecular BiomedicineBioanalytical Mass Spectrometry Facility, Röntgenstraße 20, D-48149 Münster, Germany.
authors.forename: Bingyuan
authors.initials: B
authors.lastname: Wang
authors.affiliation: Max Planck Institute for Molecular BiomedicineRöntgenstraße 20, D-48149 Münster, GermanyDZNEGerman Centre for Neurodegenerative Disorders, Gehlsheimer Straße 20, D-18147 Rostock, GermanyInstitute for Biostatistics and Informatics in Medicine and Ageing ResearchRostock University Medical Center, Ernst Heydemann-Str. 8, D-18057 Rostock, GermanyArrows Biomedical GmbHGievenbecker Weg 11, D-48149 Münster, GermanyMax Planck Institute for Molecular BiomedicineBioanalytical Mass Spectrometry Facility, Röntgenstraße 20, D-48149 Münster, GermanyMax Planck Institute for Molecular BiomedicineRöntgenstraße 20, D-48149 Münster, GermanyDZNEGerman Centre for Neurodegenerative Disorders, Gehlsheimer Straße 20, D-18147 Rostock, GermanyInstitute for Biostatistics and Informatics in Medicine and Ageing ResearchRostock University Medical Center, Ernst Heydemann-Str. 8, D-18057 Rostock, GermanyArrows Biomedical GmbHGievenbecker Weg 11, D-48149 Münster, GermanyMax Planck Institute for Molecular BiomedicineBioanalytical Mass Spectrometry Facility, Röntgenstraße 20, D-48149 Münster, Germany mboiani@mpi-muenster.mpg.de fuellen@uni-rostock.de.
authors.forename: Georg
authors.initials: G
authors.lastname: Fuellen
authors.affiliation: Max Planck Institute for Molecular BiomedicineRöntgenstraße 20, D-48149 Münster, GermanyDZNEGerman Centre for Neurodegenerative Disorders, Gehlsheimer Straße 20, D-18147 Rostock, GermanyInstitute for Biostatistics and Informatics in Medicine and Ageing ResearchRostock University Medical Center, Ernst Heydemann-Str. 8, D-18057 Rostock, GermanyArrows Biomedical GmbHGievenbecker Weg 11, D-48149 Münster, GermanyMax Planck Institute for Molecular BiomedicineBioanalytical Mass Spectrometry Facility, Röntgenstraße 20, D-48149 Münster, Germany mboiani@mpi-muenster.mpg.de fuellen@uni-rostock.de.
authors.forename: Michele
authors.initials: M
authors.lastname: Boiani
mesh_terms: D000375:Aging / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D053263:Gene Regulatory Networks; D008423:Maternal Age; D051379:Mice; D009865:Oocytes / Q000378:metabolism*; D060066:Protein Interaction Maps; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D011506:Proteins; D012333:RNA, Messenger