title: Using combined bio-omics methods to evaluate the complicated toxic effects of mixed chemical wastewater and its treated effluent.
abstract: Mixed chemical wastewaters (MCWW) from industrial park contain complex mixtures of trace contaminants, which cannot be effectively removed by wastewater treatment plants (WWTP) and have become an unignored threat to ambient environment. However, limited information is available to evaluate the complicated toxic effects of MCWW and its effluent from wastewater treatment plant (WTPE) from the perspective of bio-omics. In this study, mice were exposed to the MCWW and WTPE for 90 days and distinct differences in the hepatic transcriptome and serum metabolome were analyzed by digital gene expression (DGE) and proton nuclear magnetic resonance ((1)H-NMR) spectra, respectively. Our results indicated that disruption of lipid metabolism in liver and hepatotoxicity were induced by both MCWW and WTPE exposure. WTPE is still a health risk to the environment, which is in need of more attention. Furthermore, we demonstrated the potential ability of bio-omics approaches for evaluating toxic effects of MCWW and WTPE.
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, Jiangsu, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, Jiangsu, China.
authors.forename: Yongfeng
authors.initials: Y
authors.lastname: Deng
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, Jiangsu, China.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, Jiangsu, China. Electronic address: hqren@nju.edu.cn.
authors.forename: Hongqiang
authors.initials: H
authors.lastname: Ren
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000378:metabolism; D004784:Environmental Monitoring / Q000379:methods; D020869:Gene Expression Profiling; D050356:Lipid Metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D055432:Metabolomics / Q000379:methods*; D051379:Mice; D014158:Transcription, Genetic; D014865:Waste Disposal, Fluid / Q000379:methods; D062065:Wastewater / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000032:analysis*; D018508:Water Purification / Q000379:methods
chemical_list: D001798:Blood Proteins; D062065:Wastewater; D014874:Water Pollutants, Chemical
keywords: Metabolomics; Mice; Mixed chemical wastewater; Toxic effects; Transcriptomics