title: In vivo function of the ER-Golgi transport protein LMAN1 in photoreceptor homeostasis.
abstract: LMAN1 is a type I transmembrane protein that selectively transports its cargo proteins from ER to ER-Golgi intermediate compartment (ERGIC) and Golgi. Lman1 is a direct target of the transcription factor NRL in mouse retina. Therefore, we examined the in vivo function of LMAN1 in retina. Although Lman1 (- / -) mouse eyes did not show abnormality in histology and electroretinogram analysis at 3 months, Lman1 (- / -) retina at 6 months showed a decrease in cis-Golgi markers GM130 and GRASP65. We also observed abnormal level and location of Rhodopsin in these mice. Taken together, LMAN1 may play a role in photoreceptor gene transport and homeostasis.
authors.affiliation: Neurobiology-Neurodegeneration & Repair Laboratory, National Eye Institute, NIH, MSC0610, 6 Center Drive, 20892, Bethesda, MD, USA, haoh@mail.nih.gov.
authors.forename: Hong
authors.initials: H
authors.lastname: Hao
authors.forename: Janina
authors.initials: J
authors.lastname: Gregorski
authors.forename: Haohua
authors.initials: H
authors.lastname: Qian
authors.forename: Yichao
authors.initials: Y
authors.lastname: Li
authors.forename: Chun Y
authors.initials: CY
authors.lastname: Gao
authors.forename: Sana
authors.initials: S
authors.lastname: Idrees
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001692:Biological Transport / Q000502:physiology; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D006056:Golgi Apparatus / Q000378:metabolism*; D006706:Homeostasis / Q000502:physiology*; D037241:Mannose-Binding Lectins / Q000235:genetics / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D020419:Photoreceptor Cells, Vertebrate / Q000502:physiology*
chemical_list: C515307:ERGIC-53 protein, mouse; D037241:Mannose-Binding Lectins; D008565:Membrane Proteins