title: 7-ps optical pulse generation from a 1064-nm gain-switched laser diode and its application for two-photon microscopy.
abstract: In this study, we investigated the picosecond optical pulse generation from a 1064-nm distributed feedback laser diode under strong gain switching. The spectrum of the generated optical pulses was manipulated in two different ways: (i) by extracting the short-wavelength components of the optical pulse spectrum and (ii) by compensating for spectral chirping in the extracted mid-spectral region. Both of these methods shortened the optical pulse duration to approximately 7 ps. These optical pulses were amplified to over 20-kW peak power for two-photon microscopy. We obtained clear two-photon images of neurons in a fixed brain slice of H-line mouse expressing enhanced yellow fluorescent protein. Furthermore, a successful experiment was also confirmed for in vivo deep region H-line mouse brain neuron imaging.
authors.forename: Yuta
authors.initials: Y
authors.lastname: Kusama
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Tanushi
authors.forename: Masami
authors.initials: M
authors.lastname: Yokoyama
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Kawakami
authors.forename: Terumasa
authors.initials: T
authors.lastname: Hibi
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Kozawa
authors.forename: Tomomi
authors.initials: T
authors.lastname: Nemoto
authors.forename: Shunichi
authors.initials: S
authors.lastname: Sato
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Yokoyama