title: Isolation of a virulent Newcastle disease virus from confiscated LaSota vaccine.
abstract: Vials of Newcastle disease vaccine labeled as LaSota were confiscated by the Arizona Division of Customs and Border Protection officials. Two different avian type 1 paramyxoviruses were isolated from all three vials of vaccine submitted to the National Veterinary Services Laboratories. The LaSota strain of avian paramyxovirus type 1 virus was isolated from all three vials and analyzed by nucleotide sequence analysis. A virulent Newcastle disease virus was also present in all three vials, but in low concentration. The virulence of the Newcastle disease virus was characterized by the intracerebral chicken pathogenicity index chicken inoculation assay but could not be determined by nucleotide sequence analysis from the virus isolated from embryonating chicken eggs. The intracerebral chicken pathogenicity index value for the isolated Newcastle disease virus was 1.55. Strains of Newcastle disease virus with intracerebral pathogenicity indexes significantly above 1.0 have been found to selectively kill many types of cancer cells while not affecting normal nonneoplastic cells and are considered to be a viable option for cancer treatment in humans by alternative medical researchers; however, the treatment is not approved for use in the United States by the Food and Drug Administration. Customs and Border Protection officials have been notified of an increased risk of Newcastle disease virus entering the United States for use as a nonapproved cancer treatment. Illegal importation of Newcastle disease vaccine for vaccination of backyard poultry is also a threat. This case report emphasizes the importance of conducting chicken inoculation for complete virus pathotyping and demonstrates the need for stringent security procedures at U.S. borders to detect known livestock pathogens that may be smuggled in for use in animal agriculture and reasons unrelated to animal agriculture.
authors.forename: Janice C
authors.initials: JC
authors.lastname: Pedersen
authors.forename: Nichole L
authors.initials: NL
authors.lastname: Hines
authors.forename: Mary Lea
authors.initials: ML
authors.lastname: Killian
authors.forename: Ann S
authors.initials: AS
authors.lastname: Predgen
authors.forename: Beverly J
authors.initials: BJ
authors.lastname: Schmitt
mesh_terms: D000818:Animals; D001130:Arizona; D002645:Chickens; D003132:Commerce; D009521:Newcastle Disease / Q000473:pathology / Q000821:virology*; D009522:Newcastle disease virus / Q000145:classification / Q000276:immunology* / Q000302:isolation & purification / Q000472:pathogenicity*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D017422:Sequence Analysis, DNA / Q000662:veterinary; D014765:Viral Vaccines / Q000032:analysis*; D014774:Virulence
chemical_list: C011894:La Sota vaccine; D014765:Viral Vaccines