title: Expression of PRMT5 correlates with malignant grade in gliomas and plays a pivotal role in tumor growth in vitro.
abstract: Protein arginine methyltransferase 5 (PRMT5) catalyzes the formation of ω-NG,N'G-symmetric dimethylarginine residues on histones as well as other proteins. These modifications play an important role in cell differentiation and tumor cell growth. However, the role of PRMT5 in human glioma cells has not been characterized. In this study, we assessed protein expression profiles of PRMT5 in control brain, WHO grade II astrocytomas, anaplastic astrocytomas, and glioblastoma multiforme (GBM) by immunohistochemistry. PRMT5 was low in glial cells in control brain tissues and low grade astrocytomas. Its expression increased in parallel with malignant progression, and was highly expressed in GBM. Knockdown of PRMT5 by small hairpin RNA caused alterations of p-ERK1/2 and significantly repressed the clonogenic potential and viability of glioma cells. These findings indicate that PRMT5 is a marker of malignant progression in glioma tumors and plays a pivotal role in tumor growth.
authors.affiliation: Department of Neurology, The University of Alabama at Birmingham, FOT 1020, 1530 3rd Ave S, Birmingham, AL, 35294-3410, USA, xhan@uab.edu.
authors.forename: Xiaosi
authors.initials: X
authors.lastname: Han
authors.forename: Rong
authors.initials: R
authors.lastname: Li
authors.forename: Wenbin
authors.initials: W
authors.lastname: Zhang
authors.forename: Xiuhua
authors.initials: X
authors.lastname: Yang
authors.forename: Crystal G
authors.initials: CG
authors.lastname: Wheeler
authors.forename: Gregory K
authors.initials: GK
authors.lastname: Friedman
authors.forename: Paula
authors.initials: P
authors.lastname: Province
authors.forename: Qiang
authors.initials: Q
authors.lastname: Ding
authors.forename: Zhiying
authors.initials: Z
authors.lastname: You
authors.forename: Hassan M
authors.initials: HM
authors.lastname: Fathallah-Shaykh
authors.forename: G Yancey
authors.initials: GY
authors.lastname: Gillespie
authors.forename: Xinyang
authors.initials: X
authors.lastname: Zhao
authors.forename: Peter H
authors.initials: PH
authors.lastname: King
authors.forename: L Burt
authors.initials: LB
authors.lastname: Nabors
mesh_terms: D000328:Adult; D000368:Aged; D001120:Arginine / Q000031:analogs & derivatives / Q000378:metabolism; D001932:Brain Neoplasms / Q000378:metabolism* / Q000473:pathology; D002454:Cell Differentiation / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics / Q000502:physiology*; D003110:Colonic Neoplasms / Q000378:metabolism / Q000473:pathology; D003114:Colony-Forming Units Assay; D004848:Epithelium / Q000378:metabolism / Q000473:pathology; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D005910:Glioma / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D020935:MAP Kinase Signaling System / Q000235:genetics; D008297:Male; D008875:Middle Aged; D009474:Neurons / Q000378:metabolism; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D011484:Protein-Arginine N-Methyltransferases / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism
chemical_list: D005904:Glial Fibrillary Acidic Protein; D034741:RNA, Small Interfering; C487735:dimethylarginine; D001120:Arginine; C400529:PRMT5 protein, human; D011484:Protein-Arginine N-Methyltransferases; D010751:Phosphopyruvate Hydratase