title: Molecular arrangements and interconnected bilayer formation induced by alcohol or polyalcohol in phospholipid vesicles.
abstract: A self-assembled hybrid phospholipid vesicular system containing various penetration enhancers - ethanol, Transcutol and propylenglycol - was prepared and characterized. The effects of the different alcohol or polyalcohols structure and their concentration on the features of the assembled vesicles were evaluated using a combination of different techniques, including cryo-transmission electron microscopy, laser light scattering, differential scanning calorimetry, small- and wide-angle X-ray scattering and rheological analysis. These techniques allow explaining the structural rearrangements of the bilayer assembly due to the alcohol or polyalcohol addition. X-ray scattering studies showed that such addition at the highest concentration (20%) allowed structure modification to oligolamellar vesicles and a bilayer transition to interdigitated phase. Rheological studies confirmed the importance of alcohol or polyalcohol in the structuring dispersions probably due to a partial tilting of phosphatidylcholine acyl chains forming interdigitated and interconnected bilayer vesicles.
authors.affiliation: Dept. Scienze della Vita e dell'Ambiente, CNBS, University of Cagliari, Cagliari, Italy.
authors.forename: Maria Letizia
authors.initials: ML
authors.lastname: Manca
authors.affiliation: Dept. Scienze della Vita e dell'Ambiente, CNBS, University of Cagliari, Cagliari, Italy.
authors.forename: Ines
authors.initials: I
authors.lastname: Castangia
authors.affiliation: Dept. Chimica e Tecnologie del Farmaco, Sapienza, Università di Roma, Roma, Italy. Electronic address: pietro.matricardi@uniroma1.it.
authors.forename: Pietro
authors.initials: P
authors.lastname: Matricardi
authors.affiliation: Dept. Scienze Chimiche e Geologiche, CNBS and CSGI, University of Cagliari, Monserrato (CA), Italy.
authors.forename: Sandrina
authors.initials: S
authors.lastname: Lampis
authors.affiliation: Barcelona Centre for International Health Research (CRESIB, Hospital Clínic-Universitat de Barcelona) and Institute for Bioengineering of Catalonia (IBEC), Barcelona, Spain.
authors.forename: Xavier
authors.initials: X
authors.lastname: Fernàndez-Busquets
authors.affiliation: Dept. Scienze della Vita e dell'Ambiente, CNBS, University of Cagliari, Cagliari, Italy.
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Fadda
authors.affiliation: Dept. Scienze della Vita e dell'Ambiente, CNBS, University of Cagliari, Cagliari, Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Manconi
mesh_terms: D000438:Alcohols / Q000737:chemistry*; D055119:Elastic Modulus; D008051:Lipid Bilayers / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry* / Q000648:ultrastructure; D010743:Phospholipids / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D012542:Scattering, Radiation; D014783:Viscosity; D014965:X-Rays
chemical_list: D000438:Alcohols; D008051:Lipid Bilayers; D008081:Liposomes; D010743:Phospholipids; D011108:Polymers
keywords: (Poly)alcohols; Cryo-TEM; DSC; Liposomes; Penetration Enhancer containing Vesicle (PEVs); Rheology; SAXS