title: Patterned substrates of nano-graphene oxide mediating highly localized and efficient gene delivery.
abstract: A facile approach was developed to fabricate patterned substrates of nano-graphene oxide, demonstrating highly localized and efficient gene delivery to multiple cell lines in a substrate-mediated manner. The GO substrates served as a valid platform to preconcentrate PEI/pDNA complexes and maintain their gradual releasing for a relatively long period of time. Our approach allowed successful gene delivery in selected groups of cells on the stripe-patterned GO substrates, without transfecting their neighbor cells directly cultured on glass. These GO substrates exhibited excellent biocompatibility and enabled effective gene transfection for various cell lines including stem cells, thus promising important applications in stem cell research and tissue engineering.
authors.affiliation: Institute of Functional Nano & Soft Materials (FUNSOM), Jiangsu Key Laboratory for Carbon-Based Functional Materials & Devices, Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University , Suzhou, Jiangsu 215123, People's Republic of China.
authors.forename: Kunyang
authors.initials: K
authors.lastname: Li
authors.forename: Liangzhu
authors.initials: L
authors.lastname: Feng
authors.forename: Jianwei
authors.initials: J
authors.lastname: Shen
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhang
authors.forename: Zhuang
authors.initials: Z
authors.lastname: Liu
authors.forename: Shuit-Tong
authors.initials: ST
authors.lastname: Lee
authors.forename: Jian
authors.initials: J
authors.lastname: Liu
mesh_terms: D002460:Cell Line; D018014:Gene Transfer Techniques / Q000295:instrumentation*; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D010087:Oxides / Q000737:chemistry*; D010957:Plasmids / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013234:Stem Cells / Q000378:metabolism; D014162:Transfection
chemical_list: D010087:Oxides; D006108:Graphite