title: Association of a vascular endothelial growth factor polymorphism with the development of bronchopulmonary dysplasia in Japanese premature newborns.
abstract: Our objective was to correlate vascular endothelial growth factor (VEGF) genetic polymorphisms with the risk of bronchopulmonary dysplasia (BPD) development in premature newborns. Fifty-five newborns with BPD (BPD: median gestational age [GA]: 27 weeks, birthweight [BW]: 786 g) and 42 newborns without BPD (non-BPD: median GA: 29 weeks, BW: 1,165 g), who were born at <32 weeks gestational age and were admitted to Kobe University Hospital, were included. BPD was defined as oxygen dependency at 36 weeks postmenstrual age. Genomic DNA was extracted from the umbilical cord, cord blood, or buccal mucosa. Six VEGF genotypes (-1498T > C, -1154G > A, -634C > G, -7C > T, 936C > T, and 1612G > A) were determined by DNA sequencing. Clinical characteristics, and allele and genotype frequencies of VEGF in the BPD and non-BPD groups were analyzed. G allele frequencies in -634C > G of the BPD group were significantly higher than in the non-BPD group (66.4% vs. 50%, P = 0.02). -634C > G genotype distributions differed significantly between the BPD and non-BPD groups (BPD: CC 7%/CG 53%/GG 40%; non-BPD: CC 24%/CG 52%/GG 24%; P = 0.04). Multivariate logistic regression showed that duration of ventilation, VEGF-634G > C G alleles, and male gender were independent risk factors for BPD. In conclusion, polymorphism VEGF -634C > G may influence the risk of BPD.
authors.affiliation: Department of Pediatrics, Kobe University Hospital, Kobe, Japan.
authors.forename: Kazumichi
authors.initials: K
authors.lastname: Fujioka
authors.affiliation: Department of Pediatrics, Kobe University Hospital, Kobe, Japan.
authors.forename: Akio
authors.initials: A
authors.lastname: Shibata
authors.affiliation: Department of Pediatrics, Kobe University Hospital, Kobe, Japan.
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Yokota
authors.affiliation: Department of Pediatrics, Kobe University Hospital, Kobe, Japan.
authors.forename: Tsubasa
authors.initials: T
authors.lastname: Koda
authors.affiliation: Department of Pediatrics, Kobe University Hospital, Kobe, Japan.
authors.forename: Miwako
authors.initials: M
authors.lastname: Nagasaka
authors.affiliation: Department of Pediatrics, Kobe University Hospital, Kobe, Japan.
authors.forename: Mariko
authors.initials: M
authors.lastname: Yagi
authors.affiliation: Department of Pediatrics, Kobe University Hospital, Kobe, Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Takeshima
authors.affiliation: Department of Obstetrics and Gynecology, Kobe University Hospital, Kobe, Japan.
authors.forename: Hideto
authors.initials: H
authors.lastname: Yamada
authors.affiliation: Department of Pediatrics, Kobe University Hospital, Kobe, Japan.
authors.forename: Kazumoto
authors.initials: K
authors.lastname: Iijima
authors.affiliation: Department of Pediatrics, Kobe University Hospital, Kobe, Japan.
authors.forename: Ichiro
authors.initials: I
authors.lastname: Morioka
mesh_terms: D001997:Bronchopulmonary Dysplasia / Q000235:genetics* / Q000503:physiopathology; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D005838:Genotype; D006801:Humans; D007231:Infant, Newborn; D007234:Infant, Premature / Q000254:growth & development; D008297:Male; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012307:Risk Factors; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics*
chemical_list: D042461:Vascular Endothelial Growth Factor A