title: Distinct modes of ubiquitination of peroxisome-targeting signal type 1 (PTS1) receptor Pex5p regulate PTS1 protein import.
abstract: Peroxisome targeting signal type-1 (PTS1) receptor, Pex5p, is a key player in peroxisomal matrix protein import. Pex5p recognizes PTS1 cargoes in the cytosol, targets peroxisomes, translocates across the membrane, unloads the cargoes, and shuttles back to the cytosol. Ubiquitination of Pex5p at a conserved cysteine is required for the exit from peroxisomes. However, any potential ubiquitin ligase (E3) remains unidentified in mammals. Here, we establish an in vitro ubiquitination assay system and demonstrate that RING finger Pex10p functions as an E3 with an E2, UbcH5C. The E3 activity of Pex10p is essential for its peroxisome-restoring activity, being enhanced by another RING peroxin, Pex12p. The Pex10p·Pex12p complex catalyzes monoubiquitination of Pex5p at one of multiple lysine residues in vitro, following the dissociation of Pex5p from Pex14p and the PTS1 cargo. Several lines of evidence with lysine-to-arginine mutants of Pex5p demonstrate that Pex10p RING E3-mediated ubiquitination of Pex5p is required for its efficient export from peroxisomes to the cytosol and peroxisomal matrix protein import. RING peroxins are required for both modes of Pex5p ubiquitination, thus playing a pivotal role in Pex5p shuttling.
authors.affiliation: From the Department of Biology, Faculty of Sciences, and the Graduate School of Systems Life Sciences, Kyushu University Graduate School, Fukuoka 812-8581, Japan.
authors.forename: Kanji
authors.initials: K
authors.lastname: Okumoto
authors.affiliation: From the Department of Biology, Faculty of Sciences, and.
authors.forename: Hiromi
authors.initials: H
authors.lastname: Noda
authors.affiliation: From the Department of Biology, Faculty of Sciences, and yfujiki@kyudai.jp.
authors.forename: Yukio
authors.initials: Y
authors.lastname: Fujiki
mesh_terms: D020816:Amino Acid Motifs; D002460:Cell Line; D003600:Cytosol / Q000378:metabolism; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism; D000074401:Peroxins; D000074435:Peroxisome-Targeting Signal 1 Receptor; D020675:Peroxisomes / Q000378:metabolism; D021381:Protein Transport; D018160:Receptors, Cytoplasmic and Nuclear / Q000737:chemistry / Q000378:metabolism*; D054875:Ubiquitination
chemical_list: D008565:Membrane Proteins; C116156:PEX10 protein, human; C105251:PEX12 protein, human; D000074401:Peroxins; D000074435:Peroxisome-Targeting Signal 1 Receptor; D018160:Receptors, Cytoplasmic and Nuclear
keywords: Peroxisome Biogenesis; Peroxisomes; Pex5p; Protein Targeting; RING Peroxins; Ubiquitin; Ubiquitin Ligase; Ubiquitination