title: Septin 7 immunoexpression in papillary thyroid carcinoma: a preliminary study.
abstract: Papillary thyroid carcinoma (PTC) is the most common type among thyroid cancers. The diagnosis of PTC may be challenging when follicular variant (FVPTC) of this disease is present due to the resemblance of nuclear properties of the classical type (CVPTC). However, making use of ancillary molecular markers in the diagnosis of PTC may help. In our study, we aimed to evaluate the SEPT7 protein expression in PTC. A total of 55 paraffin block tissue samples comprising encapsulated FVPTC (FVPTC(e), n=25), and CVPTC (n=15), and benign hyperfunctioning thyroid nodules (HypN, n=15) were used in this study. Nuclear, cytoplasmic, and overall (total) SEPT7 protein expression levels were determined by using immunohistochemistry. Nuclear, cytoplasmic, and overall SEPT7 expressions (p=0.02, p=0.001, p=0.002, respectively) were significantly lower in FVPTC(e) tissues when compared to HypN. In CVPTC group, nuclear expression was significantly lower (p=0.004) while overall and cytoplasmic expressions were not changed (p>0.05). In HypN group, highest nuclear (mean=2.73), cytoplasmic (mean=2.86), and overall (mean=2.86) expression scores were detected. Significantly lower SEPT7 expression in all expressional categories in FVPTC(e) group may be a sign of different molecular signature in this type of tissue.
authors.affiliation: University of Gaziantep, Faculty of Medicine, Department of Medical Biology, Gaziantep, Turkey. Electronic address: igci@gantep.edu.tr.
authors.forename: Yusuf Ziya
authors.initials: YZ
authors.lastname: Igci
authors.affiliation: University of Gaziantep, Faculty of Medicine, Department of Pathology, Gaziantep, Turkey.
authors.forename: Suna
authors.initials: S
authors.lastname: Erkilic
authors.affiliation: University of Gaziantep, Faculty of Medicine, Department of Medical Biology, Gaziantep, Turkey.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Arslan
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis*; D002277:Carcinoma / Q000378:metabolism* / Q000473:pathology*; D002291:Carcinoma, Papillary; D018797:Cell Cycle Proteins / Q000032:analysis / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D012189:Retrospective Studies; D058112:Septins / Q000032:analysis / Q000096:biosynthesis*; D000077273:Thyroid Cancer, Papillary; D013964:Thyroid Neoplasms / Q000378:metabolism* / Q000473:pathology*; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; D018797:Cell Cycle Proteins; C113158:SEPTIN7 protein, human; D058112:Septins
keywords: Follicular variant of papillary thyroid carcinoma; Papillary thyroid carcinoma; SEPT7; Septin 7