title: Distinct molecular basis for endothelial differentiation: gene expression profiles of human mesenchymal stem cells versus umbilical vein endothelial cells.
abstract: The capacity for endothelial differentiation has been described in mesenchymal stem cells (MSC) from human bone marrow. To identify genes associated with the endothelial differentiation potential of this cell-type, and search for the optimal regulatory factors, the expression profile of MSC was compared with cDNA from primary human umbilical vein endothelial cells as controls, using cDNA chips with 4096 genes. The data were corroborated by quantitative PCR, Western blotting, and immunocytochemistry. Among the 3948 effective genes, ∼84% (3321) were co-expressed in both cell-types, and 627 were differentially expressed more than twofold in MSC versus EC. MSC highly expressed numerous stem-cell-like genes. Early development genes of endothelial cells, though not up-regulated, had a high expression in MSC, such as EDF1, MDG1, and EDG2. In contrast, mature endothelial growth and signal pathway genes, like VEGF, CXCR4, and CTNNB1, were down-regulated in MSC. In conclusion, human MSC have a distinct molecular basis for endothelial differentiation.
authors.affiliation: Department of Pathology, Zhejiang Medical College, 481 Bin Wen Road, Bin Jiang District, Hangzhou 310053, China. Electronic address: 7559220@qq.com.
authors.forename: Dandan
authors.initials: D
authors.lastname: Liu
authors.affiliation: Department of Pathology, Norman Bethune College of Medicine, Jilin University, Changchun, China. Electronic address: wangyuezeng@email.jlu.edu.cn.
authors.forename: Yuezeng
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Pathology, Zhejiang Medical College, 481 Bin Wen Road, Bin Jiang District, Hangzhou 310053, China. Electronic address: 307952548@qq.com.
authors.forename: Yilu
authors.initials: Y
authors.lastname: Ye
authors.affiliation: Department of Pathology, Zhejiang Medical College, 481 Bin Wen Road, Bin Jiang District, Hangzhou 310053, China. Electronic address: 22007882@qq.com.
authors.forename: Guoli
authors.initials: G
authors.lastname: Yin
authors.affiliation: Department of Paediatrics, Hangzhou First People's Hospital, 261 Huan Sha Road, Hangzhou 310006, China. Electronic address: 951916503@qq.com.
authors.forename: Liqiong
authors.initials: L
authors.lastname: Chen
mesh_terms: D002148:Calmodulin-Binding Proteins / Q000235:genetics; D002454:Cell Differentiation / Q000235:genetics*; D049109:Cell Proliferation; D002478:Cells, Cultured; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D050956:HSP40 Heat-Shock Proteins / Q000235:genetics; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D018832:Molecular Chaperones / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D019718:Receptors, CXCR4 / Q000235:genetics; D049368:Receptors, Lysophosphatidic Acid / Q000235:genetics; D059467:Transcriptome; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics; D051176:beta Catenin / Q000235:genetics
chemical_list: C495270:CTNNB1 protein, human; C527276:CXCR4 protein, human; D002148:Calmodulin-Binding Proteins; C478021:DNAJB9 protein, human; C115911:EDF1 protein, human; D050956:HSP40 Heat-Shock Proteins; D008565:Membrane Proteins; D018832:Molecular Chaperones; D019718:Receptors, CXCR4; D049368:Receptors, Lysophosphatidic Acid; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D051176:beta Catenin
keywords: Endothelial cell; Endothelial differentiation; Gene expression; Mesenchymal stem cell