title: Involvement of the SATB1/F-actin complex in chromatin reorganization during active cell death.
abstract: Over the past years, confirmations on the presence of actin and/or its polymerized form, F-actin, in the cell nucleus are progressively accumulating. Nevertheless, the function and localization of F-actin in the nucleus is still not fully characterized. Thus, the aim of the present study was to evaluate the association between F-actin and sequence-binding protein 1 (SATB1) and their involvement in chromatin remodeling associated with active cell death. Both SATB1 and F-actin were colocalized in the transcriptional active regions of the cell nucleus and a functional interaction was observed between SATB1 and higher-organized nuclear F-actin structures at the border between condensed and decondensed chromatin. These results extend the knowledge on the role of SATB1 and nuclear F-actin in three-dimensional chromatin organization and their functions during active cell death. Additionally, this study opens the discussion on the involvement of the SATB1/F-actin functional complex in active cell death; further studies are required to fully elucidate these issues.
authors.affiliation: Department and Clinic of Dermatology, Sexually Transmitted Diseases and Immunodermatology, Nicolaus Copernicus University in Toruń, Collegium Medicum in Bydgoszcz, 85-092 Bydgoszcz, Poland.
authors.forename: Dariusz
authors.initials: D
authors.lastname: Grzanka
authors.affiliation: Department of Histology and Embryology, Nicolaus Copernicus University in Toruń, Collegium Medicum in Bydgoszcz, 85-092 Bydgoszcz, Poland.
authors.forename: Maciej
authors.initials: M
authors.lastname: Gagat
authors.affiliation: Department of Histology and Embryology, Nicolaus Copernicus University in Toruń, Collegium Medicum in Bydgoszcz, 85-092 Bydgoszcz, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Izdebska
mesh_terms: D000199:Actins / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western; D016466:CHO Cells; D016923:Cell Death / Q000187:drug effects*; D002467:Cell Nucleus / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism*; D003412:Cricetulus; D004317:Doxorubicin / Q000494:pharmacology; D006801:Humans; D047468:Immunoprecipitation; D036961:Matrix Attachment Region Binding Proteins / Q000378:metabolism*; D011485:Protein Binding / Q000187:drug effects
chemical_list: D000199:Actins; D002843:Chromatin; D036961:Matrix Attachment Region Binding Proteins; C076189:SATB1 protein, human; D004317:Doxorubicin