title: Enhanced production of Aspergillus tamarii lipase for recovery of fat from tannery fleshings.
abstract: The influence of various oil cakes has been investigated for high level production of lipase using Aspergillus tamarii MTCC 5152. By solid state fermentation in wheat bran containing 2.5% w/w gingili oil cake at 70% v/w moisture content the fungus produced a maximal yield of lipase (758 ± 3.61 u/g) after 5 days of incubation using 2% v/w inoculum containing 10(6) spores/mL. Wheat bran and gingili oil cake with supplementation of gingili oil (1.0% w/w), glucose (0.5% w/w) and peptone (0.5% w/w) gives an increased enzyme production of 793 ± 6.56 u/g. The enzyme shows maximum activity at pH 7.0, temperature 50 °C and was stable between the pH 5.0-8.0 and temperature up to 60 °C. Crude lipase (3%) applied to tannery fleshing shows 92% fat solubility. The results demonstrate that fat obtained from tannery fleshing, a by-product of the leather industry has a high potential for biodiesel production and the proteinaceous residue obtained can be used as animal feed.
authors.affiliation: Department of Microbiology, Central Leather Research Institute, Tamil Nadu, New Delhi.
authors.forename: A
authors.initials: A
authors.lastname: Dayanandan
authors.affiliation: Department of Microbiology, Central Leather Research Institute, Tamil Nadu, New Delhi.
authors.forename: S Hilda Vimala
authors.initials: SH
authors.lastname: Rani
authors.affiliation: Department of Microbiology, Central Leather Research Institute, Tamil Nadu, New Delhi.
authors.forename: M
authors.initials: M
authors.lastname: Shanmugavel
authors.affiliation: Department of Microbiology, Central Leather Research Institute, Tamil Nadu, New Delhi.
authors.forename: A
authors.initials: A
authors.lastname: Gnanamani
authors.affiliation: Department of Microbiology, Central Leather Research Institute, Tamil Nadu, New Delhi.
authors.forename: G Suseela
authors.initials: GS
authors.lastname: Rajakumar
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D001230:Aspergillus / Q000201:enzymology*; D003470:Culture Media / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000378:metabolism*; D013696:Temperature
chemical_list: D003470:Culture Media; D008049:Lipase
keywords: fat hydrolysis; lipase; oil cakes; tannery fleshing; wheat bran