title: Two novel clerodane diterpenenes with NGF-potentiating activities from the twigs of Croton yanhuii.
abstract: Nerve growth factor (NGF) and analog reagents to promote the neurite outgrowth of nerve cells against the neuron degeneration are expected to be potentially useful for the medical treatment of Alzheimer's disease. In our focus on the discovery of bioactive diterpenes, we investigated the chemical constituents of the plant Croton yanhuii. This investigation led to the isolation and identification of two novel clerodane diterpenes (1 and 2). Their structures were elucidated on the basis of extensive 1D and 2D NMR (COSY, HMQC, HMBC, and NOESY) and mass (ESIMS and HR-ESIMS) spectroscopic data analyses. Further biological screenings showed that both of the compounds enhanced NGF-mediated neurite outgrowth from PC12 cells.
authors.affiliation: College of Pharmacy and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin 300071, China; College of Pharmacy, Harbin University of Commerce, Harbin 150076, China.
authors.forename: Yihang
authors.initials: Y
authors.lastname: Sun
authors.affiliation: College of Pharmacy and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin 300071, China.
authors.forename: Meicheng
authors.initials: M
authors.lastname: Wang
authors.affiliation: College of Pharmacy and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin 300071, China.
authors.forename: Quanhui
authors.initials: Q
authors.lastname: Ren
authors.affiliation: College of Pharmacy and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin 300071, China.
authors.forename: Shen
authors.initials: S
authors.lastname: Li
authors.affiliation: College of Pharmacy and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin 300071, China. Electronic address: xujing611@nankai.edu.cn.
authors.forename: Jing
authors.initials: J
authors.lastname: Xu
authors.affiliation: Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai 980-8578, Japan.
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Ohizumi
authors.affiliation: College of Pharmacy and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin 300071, China.
authors.forename: Chunfeng
authors.initials: C
authors.lastname: Xie
authors.affiliation: School of Medicine, Nankai University, Tianjin 300071, China.
authors.forename: Da-Qing
authors.initials: DQ
authors.lastname: Jin
authors.affiliation: College of Pharmacy and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin 300071, China. Electronic address: victgyq@nankai.edu.cn.
authors.forename: Yuanqiang
authors.initials: Y
authors.lastname: Guo
mesh_terms: D000544:Alzheimer Disease / Q000188:drug therapy*; D000818:Animals; D029802:Croton / Q000737:chemistry*; D045785:Diterpenes, Clerodane / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology / Q000627:therapeutic use; D004357:Drug Synergism; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D020932:Nerve Growth Factor / Q000378:metabolism; D016501:Neurites / Q000187:drug effects*; D016716:PC12 Cells; D010946:Plants, Medicinal; D051381:Rats
chemical_list: D045785:Diterpenes, Clerodane; C000594199:crotonpene A; C000594200:crotonpene B; D020932:Nerve Growth Factor
keywords: Alzheimer's disease; Clerodane diterpene; Croton yanhuii; Nerve growth factor (NGF)-potentiating activity