title: Characterization of heparin-protein interaction by saturation transfer difference (STD) NMR.
abstract: The binding affinity and specificity of heparin to proteins is widely recognized to be sulfation-pattern dependent. However, for the majority of heparin-binding proteins (HBPs), it still remains unclear what moieties are involved in the specific binding interaction. Here, we report our study using saturation transfer difference (STD) nuclear magnetic resonance (NMR) to map out the interactions of synthetic heparin oligosaccharides with HBPs, such as basic fibroblast growth factor (FGF2) and fibroblast growth factor 10 (FGF10), to provide insight into the critical epitopes of heparin ligands involved. The irradiation frequency of STD NMR was carefully chosen to excite the methylene protons so that enhanced sensitivity was obtained for the heparin-protein complex. We believe this approach opens up additional application avenues to further investigate heparin-protein interactions.
authors.affiliation: Momenta Pharmaceuticals, Inc. 475 West Kendall Street, 02142, Cambridge, MA, USA.
authors.forename: Fei
authors.initials: F
authors.lastname: Yu
authors.forename: Sucharita
authors.initials: S
authors.lastname: Roy
authors.forename: Enrique
authors.initials: E
authors.lastname: Arevalo
authors.forename: John
authors.initials: J
authors.lastname: Schaeck
authors.forename: Jason
authors.initials: J
authors.lastname: Wang
authors.forename: Kimberly
authors.initials: K
authors.lastname: Holte
authors.forename: Jay
authors.initials: J
authors.lastname: Duffner
authors.forename: Nur Sibel
authors.initials: NS
authors.lastname: Gunay
authors.forename: Ishan
authors.initials: I
authors.lastname: Capila
authors.forename: Ganesh V
authors.initials: GV
authors.lastname: Kaundinya
mesh_terms: D051526:Fibroblast Growth Factor 10 / Q000737:chemistry / Q000378:metabolism*; D016222:Fibroblast Growth Factor 2 / Q000737:chemistry / Q000378:metabolism*; D006493:Heparin / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D011485:Protein Binding; D020349:Surface Plasmon Resonance
chemical_list: C496463:FGF10 protein, human; D051526:Fibroblast Growth Factor 10; D016222:Fibroblast Growth Factor 2; D006493:Heparin