title: The role of PinX1 in growth control of breast cancer cells and its potential molecular mechanism by mRNA and lncRNA expression profiles screening.
abstract: As a major tumor suppressor gene, the role of PinX1 in breast cancer and its molecular mechanism remain unclear. In this study, overexpression of PinX1 was generated in 3 breast cancer cell lines, and knockdown of PinX1 was performed in a nontumorigenic breast cell line. The regulation of PinX1 on cell proliferation and cell cycle was observed. A microarray-based lncRNA and mRNA expression profile screening was also performed. We found a lower growth rate, G0/G1 phase arrest, and S phase inhibition in the PinX1 overexpressed breast cancer cells, while a higher growth rate, decreased G0/G1 phase, and increased S phase rate in the PinX1 knocked-down nontumorigenic breast cell. A total of 977 mRNAs and 631 lncRNAs were identified as differentially expressed transcripts between PinX1 overexpressed and control MCF-7 cells. Further analysis identified the involvement of these mRNAs in 52 cancer related pathways and various other biological processes. 11 enhancer-like lncRNAs and 25 lincRNAs with their adjacent mRNA pairs were identified as coregulated transcripts. Our results confirmed the role of PinX1 as a major tumor suppressor gene in breast cancer cell lines and provided information for further research on the molecular mechanisms of PinX1 in tumorigenesis.
authors.affiliation: Institute of Genetic Engineering, Southern Medical University, Guangzhou 510515, China.
authors.forename: Rong
authors.identifier: 0000-0001-6057-2439
authors.initials: R
authors.lastname: Shi
authors.affiliation: Institute of Genetic Engineering, Southern Medical University, Guangzhou 510515, China.
authors.forename: Jue-Yu
authors.identifier: 0000-0002-3799-3718
authors.initials: JY
authors.lastname: Zhou
authors.affiliation: Institute of Genetic Engineering, Southern Medical University, Guangzhou 510515, China.
authors.forename: Hui
authors.identifier: 0000-0003-2926-5734
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Nan Fang Hospital, Southern Medical University, Guangzhou 510515, China.
authors.forename: Zhen
authors.identifier: 0000-0001-7127-3215
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Institute of Genetic Engineering, Southern Medical University, Guangzhou 510515, China.
authors.forename: Sang-Hua
authors.identifier: 0000-0002-7250-7826
authors.initials: SH
authors.lastname: Liang
authors.affiliation: Institute of Genetic Engineering, Southern Medical University, Guangzhou 510515, China.
authors.forename: Wen-Ling
authors.identifier: 0000-0002-4686-7596
authors.initials: WL
authors.lastname: Zheng
authors.affiliation: Institute of Genetic Engineering, Southern Medical University, Guangzhou 510515, China.
authors.forename: Wen-Li
authors.identifier: 0000-0003-1367-4453
authors.initials: WL
authors.lastname: Ma
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology*; D018797:Cell Cycle Proteins; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D063990:Gene Ontology; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D062085:RNA, Long Noncoding / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D014162:Transfection; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D018797:Cell Cycle Proteins; C440808:PINX1 protein, human; D062085:RNA, Long Noncoding; D012333:RNA, Messenger; D025521:Tumor Suppressor Proteins