title: [Effect of antibacterial peptide LL-37 on the integrity of Acinetobacter baumannii biofilm].
abstract: OBJECTIVE
  To investigate the effect of antibacterial peptide LL-37 on the integrity of Acinetobacter baumannii biofilm.
  
  
  METHODS
  A model of Acinetobacter baumannii biofilm in vitro was constructed by plates and crystal violet staining method, and the minimal inhibitory concentration of LL-37 was determined by broth dilution. The biofilm morphology was observed by scanning electron microscopy and biofilm formation was analyzed by the crystal violet staining of the adherent biofilm in the presence of antibacterial peptide LL-37.
  
  
  RESULTS
  In the Acinetobacter baumannii biofilm model, the minimal inhibitory concentration of LL-37 was 64 µg/ml; LL-37 caused structural damage of the biofilm at a low concentration of 2.5 µg/ml. The biofilm was decreased gradually as the concentration of LL-37 increased.
  
  
  CONCLUSIONS
  LL-37 even at a concentration far below its minimal inhibitory concentration can cause structural damage of Acinetobacter baumannii biofilm in vitro.
authors.affiliation: Department of Burns, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. E-mail: shipengwei_069@163.com.
authors.forename: Pengwei
authors.initials: P
authors.lastname: Shi
authors.forename: Yanbin
authors.initials: Y
authors.lastname: Gao
authors.forename: Zhiyang
authors.initials: Z
authors.lastname: Lu
authors.forename: Lei
authors.initials: L
authors.lastname: Yang
mesh_terms: D040981:Acinetobacter baumannii / Q000187:drug effects* / Q000502:physiology*; D023181:Antimicrobial Cationic Peptides; D018441:Biofilms / Q000187:drug effects*; D054804:Cathelicidins / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests
chemical_list: D023181:Antimicrobial Cationic Peptides; D054804:Cathelicidins