title: A novel label-free electrochemical sensor for Hg2+ based on the catalytic formation of metal nanoparticle.
abstract: A novel label-free electrochemical sensor has been developed for the highly sensitive and selective detection of Hg(2+). Hg(2+) were first captured on the modified electrode surface through the specific thymine-Hg(2+)-thymine (T-Hg(2+)-T) coordination and then through the catalytic HAuCl4/NH2OH reaction for the formation of gold nanoparticles (AuNPs) as signal reporter. The formed AuNPs could be directly detected by stripping voltammetry. By the introduction of graphene to accelerate electron transfer and amplify the electrochemical signal, a detection limit as low as 0.06 nM could be obtained for Hg(2+). Compared with the traditional metal nanoparticles (NPs)-based method, this sensor avoids the labeling of the DNA probe with NP tags, only one unlabeled T-rich DNA sequence was needed, which greatly reduced the cost and simplified the sensing procedure.
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou, Fujian 350108, China.
authors.forename: Shurong
authors.initials: S
authors.lastname: Tang
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou, Fujian 350108, China; Analytical and Testing Center, The Sport Science Research Center, Fuzhou University, Fuzhou, Fujian 350002, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Tong
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou, Fujian 350108, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Lu
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou, Fujian 350108, China; Longyan Entry-Exit Inspection and Quarantine Bureau of P.R.C, Longyan, Fujian 364000, China.
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Chen
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou, Fujian 350108, China.
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Yan
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou, Fujian 350108, China; Analytical and Testing Center, The Sport Science Research Center, Fuzhou University, Fuzhou, Fujian 350002, China. Electronic address: zlan@fzu.edu.cn.
authors.forename: Lan
authors.initials: L
authors.lastname: Zhang
mesh_terms: D015374:Biosensing Techniques / Q000379:methods; D002384:Catalysis; D002413:Cations, Divalent / Q000032:analysis; D015342:DNA Probes / Q000737:chemistry; D055664:Electrochemical Techniques / Q000379:methods*; D006108:Graphite / Q000737:chemistry; D057230:Limit of Detection; D008628:Mercury / Q000032:analysis*; D053768:Metal Nanoparticles / Q000737:chemistry*
chemical_list: D002413:Cations, Divalent; D015342:DNA Probes; D006108:Graphite; D008628:Mercury
keywords: Electrochemical sensor; Gold nanoparticle; Hg(2+)detection; Label-free