title: miR-99a and -99b inhibit cervical cancer cell proliferation and invasion by targeting mTOR signaling pathway.
abstract: MicroRNAs were demonstrated to play an important role in the regulation of gene expression. Here, we showed that miR-99a and -99b (miR-99a/b) were down-regulated in human cervical cancer patient tissues and were negatively related with lymphatic metastasis. In addition, overexpression of miR-99a/b inhibited cell growth and invasion, whereas suppression of miR-99a/b yielded the reverse phenotype. Dual luciferase report assay revealed that mTOR was identified as a novel target gene of both miR-99a and -99b. Altogether, these results suggested that miR-99a/b directly and negatively regulated mTOR expression in cervical cancer cells, and enforced the importance of miR-99a/b and their targets in the malignant phenotypes of cervical carcinogenesis.
authors.affiliation: Department of Obstetrics and Gynecology, the Second Affiliated Hospital, Medical School of Xi'an Jiaotong University, 157 West Fifth Road, Xi'an, 710004, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Lihua
authors.initials: L
authors.lastname: Chang
authors.forename: Zhaorong
authors.initials: Z
authors.lastname: Li
authors.forename: Qing
authors.initials: Q
authors.lastname: Gao
authors.forename: Dongge
authors.initials: D
authors.lastname: Cai
authors.forename: Yingfang
authors.initials: Y
authors.lastname: Tian
authors.forename: Lingxia
authors.initials: L
authors.lastname: Zeng
authors.forename: Mu
authors.initials: M
authors.lastname: Li
mesh_terms: D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D002465:Cell Movement; D049109:Cell Proliferation; D005260:Female; D006801:Humans; D008156:Luciferases / Q000378:metabolism; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000235:genetics*; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D058570:TOR Serine-Threonine Kinases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*
chemical_list: C561679:MIRN99 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D008156:Luciferases; C546842:MTOR protein, human; D058570:TOR Serine-Threonine Kinases