title: Enzymatic control of chitosan gelation for delivery of periodontal ligament cells.
abstract: The aim of this study is to optimize enzymatic control over gelation of chitosan-based hydrogels for the delivery of periodontal ligament cells (PDLCs). The results reveal that the gelation time, strength, and degradation rate of the chitosan hydrogels can be controlled precisely by variation of the urea and urease concentrations. PDLCs remain viable inside these hydrogels for up to 30 days. Cells released from the hydrogel upon degradation and collected after 3, 15, and 30 days are able to form colonies and osteogenically differentiate. In conclusion, the enzymatic control over the gelation of chitosan hydrogels offers options for the delivery of PDLCs.
authors.affiliation: Department of Biomaterials, Radboud University Medical Center, 309 Dentistry, PO Box 9101, 6500 HB, Nijmegen, The Netherlands; Department of Periodontology, Shandong University, Jinan, 250012, Shandong, P. R. China.
authors.forename: Xiang-Zhen
authors.initials: XZ
authors.lastname: Yan
authors.forename: Arnold W G
authors.initials: AW
authors.lastname: Nijhuis
authors.forename: Jeroen J J P
authors.initials: JJ
authors.lastname: van den Beucken
authors.forename: Sanne K
authors.initials: SK
authors.lastname: Both
authors.forename: John A
authors.initials: JA
authors.lastname: Jansen
authors.forename: Sander C G
authors.initials: SC
authors.lastname: Leeuwenburgh
authors.forename: Fang
authors.initials: F
authors.lastname: Yang
mesh_terms: D000293:Adolescent; D000469:Alkaline Phosphatase / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D048271:Chitosan / Q000494:pharmacology*; D003114:Colony-Forming Units Assay; D055119:Elastic Modulus / Q000187:drug effects; D005260:Female; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010513:Periodontal Ligament / Q000166:cytology* / Q000187:drug effects; D012212:Rheology / Q000187:drug effects; D013997:Time Factors; D014510:Urease / Q000378:metabolism*; D014867:Water / Q000737:chemistry
chemical_list: D014867:Water; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D048271:Chitosan; D000469:Alkaline Phosphatase; D014510:Urease
keywords: cell encapsulation; chitosan; hydrogels; periodontium