title: Effects of novel hydroxyapatite-based 3D biomaterials on proliferation and osteoblastic differentiation of mesenchymal stem cells.
abstract: The aim of this study was to examine the differential capacity of isolated dental pulp stem cells (SHED) cultured onto four different scaffold materials. The differential potential of isolated SHED was examined on the following scaffolds: porous hydroxyapatite (pHAP) alone or combined with three polymers [polylactic-co-glycolic acid (PLGA), alginate, and ethylene vinylacetate / ethylene vinylversatate (EVA/EVV)]. SHED were isolated by "outgrowth" method and characterized by the flow cytometry. Viability of cells grown with scaffolds was assessed by MTT and LDH assays. No significant cytotoxic effect of any of the tested materials was shown. Staining with alizarin red and estimated alkaline phosphatase activity to identify differentiation, demonstrated osteoblastic phenotype of SHED and newly deposited and mineralized extra cellular matrix (ECM) in presence of all tested scaffolds. The developed ECM seen at scanning electronic micrographs additionally confirmed the osteogenic differentiation and biocompatibility between cells and materials. In summary, all studied biomaterials are suitable carriers for proliferation and osteoblastic differentiation of dental pulp mesenchymal stem cells in vitro.
authors.affiliation: Centre of Research Excellence in Nutrition and Metabolism, Institute for Medical Research, University of Belgrade, Belgrade, 11000, Serbia.
authors.forename: Ivana
authors.initials: I
authors.lastname: Karadzic
authors.forename: Vesna
authors.initials: V
authors.lastname: Vucic
authors.forename: Vukoman
authors.initials: V
authors.lastname: Jokanovic
authors.forename: Jasmina
authors.initials: J
authors.lastname: Debeljak-Martacic
authors.forename: Dejan
authors.initials: D
authors.lastname: Markovic
authors.forename: Snjezana
authors.initials: S
authors.lastname: Petrovic
authors.forename: Marija
authors.initials: M
authors.lastname: Glibetic
mesh_terms: D001672:Biocompatible Materials; D002454:Cell Differentiation; D049109:Cell Proliferation; D002648:Child; D017886:Durapatite; D005434:Flow Cytometry; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology*; D011108:Polymers / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D011108:Polymers; D017886:Durapatite
keywords: bone tissue engineering; dental pulp mesenchymal stem cells; hydroxyapatite; osteogenic differentiation; scaffolds