title: Impact of uncontrolled freezing and long-term storage of peripheral blood stem cells at - 80 °C on haematopoietic recovery after autologous transplantation. Report from two centres.
abstract: Controlled-rate freezing and storage in vapour phase nitrogen are used by most transplantation teams for the cryopreservation and storage of peripheral blood haematopoietic stem cells (PBSC). In this study, we analysed 666 autologous PBSC transplants after uncontrolled freezing and storage of PBSC at -80 °C. Statistical analysis showed that neutrophil recovery was associated with both the infused CD34(+) cell dose (P=0.01) and the post transplantation use of growth factors (P<0.001) and that platelet recovery was associated with the infused CD34(+) cell dose (P<0.001) and with the diagnosis (P=0.02). We analysed three groups according to the duration of the cryopreservation period (less than 6 months, between 6 and 12 months or more than 1 year). Haematopoietic recovery was not found to be adversely affected by longer storage at -80 °C. The haematopoietic recoveries of 50 pairs of sequential transplantations from the same PBSC mobilization were analysed. Despite prolonged cryopreservation, there were no statistically significant differences in neutrophil (P=0.09) or platelet (P=0.22) recovery in the second compared with the first transplant. In conclusion, the long-term storage of PBSC at -80 °C after uncontrolled-rate freezing is an easy and comparatively inexpensive cryopreservation method that leads to successful haematopoietic recovery even after prolonged storage.
authors.affiliation: Laboratoire d'hématologie, Centre Hospitalier de Jolimont, Haine Saint Paul, Belgium.
authors.forename: G
authors.initials: G
authors.lastname: Detry
authors.affiliation: Service de Thérapie cellulaire et Hématologie Clinique, Université d'Auvergne, CHU Estaing, Clermont-Ferrand, France.
authors.forename: L
authors.initials: L
authors.lastname: Calvet
authors.affiliation: Service d'hématologie, Centre Hospitalier de Jolimont, Haine Saint Paul, Belgium.
authors.forename: N
authors.initials: N
authors.lastname: Straetmans
authors.affiliation: Service de Thérapie cellulaire et Hématologie Clinique, Université d'Auvergne, CHU Estaing, Clermont-Ferrand, France.
authors.forename: A
authors.initials: A
authors.lastname: Cabrespine
authors.affiliation: Service d'hématologie, Centre Hospitalier de Jolimont, Haine Saint Paul, Belgium.
authors.forename: C
authors.initials: C
authors.lastname: Ravoet
authors.affiliation: Service de Thérapie cellulaire et Hématologie Clinique, Université d'Auvergne, CHU Estaing, Clermont-Ferrand, France.
authors.forename: J O
authors.initials: JO
authors.lastname: Bay
authors.affiliation: Service d'hématologie, Centre Hospitalier de Jolimont, Haine Saint Paul, Belgium.
authors.forename: H
authors.initials: H
authors.lastname: Petre
authors.affiliation: Centre régional de cancérologie et thérapie cellulaire pédiatrique, Centre de Biothérapie d'Auvergne, CHU Estaing, Clermont-Ferrand, France.
authors.forename: C
authors.initials: C
authors.lastname: Paillard
authors.affiliation: Laboratoire d'hématologie, Centre Hospitalier de Jolimont, Haine Saint Paul, Belgium.
authors.forename: B
authors.initials: B
authors.lastname: Husson
authors.affiliation: Centre régional de cancérologie et thérapie cellulaire pédiatrique, Centre de Biothérapie d'Auvergne, CHU Estaing, Clermont-Ferrand, France.
authors.forename: E
authors.initials: E
authors.lastname: Merlin
authors.affiliation: Laboratoire d'hématologie, Centre Hospitalier de Jolimont, Haine Saint Paul, Belgium.
authors.forename: L
authors.initials: L
authors.lastname: Boon-Falleur
authors.affiliation: Service de Thérapie cellulaire et Hématologie Clinique, Université d'Auvergne, CHU Estaing, Clermont-Ferrand, France.
authors.forename: O
authors.initials: O
authors.lastname: Tournilhac
authors.affiliation: Service d'hématologie, Centre Hospitalier de Jolimont, Haine Saint Paul, Belgium.
authors.forename: A
authors.initials: A
authors.lastname: Delannoy
authors.affiliation: Centre régional de cancérologie et thérapie cellulaire pédiatrique, Centre de Biothérapie d'Auvergne, CHU Estaing, Clermont-Ferrand, France.
authors.forename: P
authors.initials: P
authors.lastname: Halle
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D064592:Autografts; D001530:Belgium; D001793:Blood Preservation / Q000379:methods*; D002648:Child; D002675:Child, Preschool; D015925:Cryopreservation / Q000379:methods*; D005260:Female; D005602:France; D019337:Hematologic Neoplasms / Q000628:therapy; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D036102:Peripheral Blood Stem Cell Transplantation; D012189:Retrospective Studies; D013997:Time Factors; D055815:Young Adult