title: Prestaining of glycoproteins in sodium dodecyl sulfate polyacrylamide gels by dansylhydrazine.
abstract: A new fluorescent prestaining method for gel-separated glycoproteins in 1D and 2D SDS-PAGE was developed by using dansylhydrazine in this study. The prestained gels could be easily imaged after electrophoresis without any time-consuming steps needed for poststains. As low as 4-8 ng glycoproteins (transferrin, α1-acid glycoprotein) could be selectively detected, which is comparable to that of Pro-Q Emerald 488, one of the most commonly used glycoprotein stain. In addition, a subsequent study of deglycosylation, glycoprotein affinity isolation, and LC-MS/MS analysis was performed to confirm the specificity of the newly developed method.
authors.affiliation: Department of histology and embryology, Institute of neuroscience, Wenzhou Medical University, Wenzhou, Zhejiang, P. R. China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.forename: Xuan
authors.initials: X
authors.lastname: Zhou
authors.forename: Qing
authors.initials: Q
authors.lastname: Yu
authors.forename: Yuanmeng
authors.initials: Y
authors.lastname: Duan
authors.forename: Binbin
authors.initials: B
authors.lastname: Huang
authors.forename: Guoying
authors.initials: G
authors.lastname: Hong
authors.forename: Ayi
authors.initials: A
authors.lastname: Zhou
authors.forename: Litai
authors.initials: L
authors.lastname: Jin
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D000595:Amino Acid Sequence; D001798:Blood Proteins / Q000032:analysis / Q000302:isolation & purification*; D003619:Dansyl Compounds / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005456:Fluorescent Dyes / Q000032:analysis; D006023:Glycoproteins / Q000032:analysis / Q000302:isolation & purification*; D006801:Humans; D006834:Hydrazines / Q000737:chemistry*; D057230:Limit of Detection; D008969:Molecular Sequence Data; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D013194:Staining and Labeling / Q000379:methods
chemical_list: D000180:Acrylic Resins; D001798:Blood Proteins; D003619:Dansyl Compounds; D005456:Fluorescent Dyes; D006023:Glycoproteins; D006834:Hydrazines; C016680:polyacrylamide gels; C027913:dansyl hydrazine; D012967:Sodium Dodecyl Sulfate
keywords: Dansylhydrazine; Electrophoresis; Glycoprotein detection; Prestain; Technology