title: Failure of Tooth Formation Mediated by miR-135a Overexpression via BMP Signaling.
abstract: MicroRNAs (miRNAs) are known to regulate a variety of gene functions in many tissues and organs, but their expression and function in tooth development are not well-understood. A specific miRNA, miR-135a, was determined to be highly expressed at the bud stage. Interestingly, after the cap stage, miR-135a was expressed in the epithelium and mesenchyme but not in the inner enamel epithelium. To identify the relationship between miR-135a and its putative target genes, Bmpr-Ia and Bmpr-Ib, in early tooth development, miR-135a was ectopically overexpressed with a lentivirus. This overexpression resulted in the repression of Bmpr-Ia and Bmpr-Ib. Furthermore, miR-135a inhibited both Bmpr-Ia and Bmpr-Ib transcription. BMP2 proteins were expressed ectopically in tooth germs during the cap stage to determine the relationship between miR-135a and BMP signaling in early tooth development. When miR-135a was ectopically expressed, no tooth formation was observed after 4 wk of incubation in the kidney capsule. This study suggested that Bmp signaling, specifically Bmpr-Ia and Bmpr-Ib, regulates tooth formation via miR-135a.
authors.affiliation: Division in Anatomy and Developmental Biology, Department of Oral Biology, Oral Science Research Center, BK21 PLUS Project, Yonsei University College of Dentistry, Seoul, Korea blueleah@yuhs.ac hsjung@yuhs.ac.
authors.forename: E J
authors.initials: EJ
authors.lastname: Kim
authors.affiliation: Division in Anatomy and Developmental Biology, Department of Oral Biology, Oral Science Research Center, BK21 PLUS Project, Yonsei University College of Dentistry, Seoul, Korea.
authors.forename: M J
authors.initials: MJ
authors.lastname: Lee
authors.affiliation: Division in Anatomy and Developmental Biology, Department of Oral Biology, Oral Science Research Center, BK21 PLUS Project, Yonsei University College of Dentistry, Seoul, Korea.
authors.forename: L
authors.initials: L
authors.lastname: Li
authors.affiliation: Division in Anatomy and Developmental Biology, Department of Oral Biology, Oral Science Research Center, BK21 PLUS Project, Yonsei University College of Dentistry, Seoul, Korea.
authors.forename: K S
authors.initials: KS
authors.lastname: Yoon
authors.affiliation: Graduate School of Biomedical Science and Engineering, College of Medicine, Hanyang University, Seoul, Korea.
authors.forename: K S
authors.initials: KS
authors.lastname: Kim
authors.affiliation: Division in Anatomy and Developmental Biology, Department of Oral Biology, Oral Science Research Center, BK21 PLUS Project, Yonsei University College of Dentistry, Seoul, Korea Oral Biosciences, Faculty of Dentistry, The University of Hong Kong, Hong Kong SAR blueleah@yuhs.ac hsjung@yuhs.ac.
authors.forename: H S
authors.initials: HS
authors.lastname: Jung
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000235:genetics / Q000494:pharmacology; D052005:Bone Morphogenetic Protein Receptors, Type I / Q000235:genetics*; D004848:Epithelium / Q000378:metabolism; D005786:Gene Expression Regulation / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics; D016086:Lentivirus / Q000235:genetics; D008297:Male; D008648:Mesoderm / Q000378:metabolism; D051379:Mice; D008819:Mice, Nude; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000235:genetics*; D009805:Odontogenesis / Q000235:genetics*; D011994:Recombinant Proteins / Q000494:pharmacology; D015398:Signal Transduction / Q000235:genetics*; D014083:Tooth Germ / Q000187:drug effects / Q000378:metabolism / Q000637:transplantation; D014158:Transcription, Genetic / Q000235:genetics; D016212:Transforming Growth Factor beta / Q000494:pharmacology
chemical_list: C525719:Bmp2 protein, mouse; D055396:Bone Morphogenetic Protein 2; D035683:MicroRNAs; C549289:Mirn135 microRNA, mouse; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; C499469:recombinant human bone morphogenetic protein-2; C498212:Bmpr1a protein, mouse; C498215:Bmpr1b protein, mouse; D052005:Bone Morphogenetic Protein Receptors, Type I
keywords: Bmp receptors; lentivirus; miRNA expression; miRNA inhibitor; microRNA; tooth development