title: The characteristics of chitinase expression in Aeromonas schubertii.
abstract: In this study, chitinase activity in an incubation broth of Aeromonas schubertii was measured using colloidal chitin azure as the substrate. More specifically, the induction of chitinases due to amendment with various carbon sources was examined. The highest chitinase activity was found following amendment with 0.5-1.0 % chitin powder, whereas the activity increased negligibly due to amendment with other carbon sources, such as glucose, GlcNAc, GlcN, sorbitol, sucrose, cellulose, or starch. The chitinase activity induced by the chitin powder was suppressed when the glucose, GlcNAc, GlcN, or starch was added simultaneously to the medium but was not suppressed by the addition of sorbitol, sucrose, or cellulose. The activity of chitinase in the crude extract was also not directly inhibited by glucose. Taken together, these findings suggest that the induction of chitinase activity depends on the acquisition of suitable carbon sources from the environment and that induction occurs at a regulatory level.
authors.affiliation: Department of Environment and Biotechnology, Refining and Manufacturing Research Institute, CPC Corporation, 217 Min-Sheng S. Rd, Chiayi, 60051, Taiwan.
authors.forename: Jeen-Kuan
authors.initials: JK
authors.lastname: Chen
authors.forename: Chia-Rui
authors.initials: CR
authors.lastname: Shen
authors.forename: Chao-Lin
authors.initials: CL
authors.lastname: Liu
mesh_terms: D000333:Aeromonas / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D001709:Biotechnology / Q000379:methods*; D002244:Carbon / Q000494:pharmacology; D002686:Chitin / Q000737:chemistry / Q000378:metabolism; D002688:Chitinases / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D004790:Enzyme Induction / Q000187:drug effects; D015870:Gene Expression / Q000187:drug effects; D005947:Glucose / Q000494:pharmacology; D010316:Particle Size
chemical_list: D002686:Chitin; D002244:Carbon; D002688:Chitinases; D005947:Glucose