title: Development of a growth-hormone-conjugated nanodiamond complex for cancer therapy.
abstract: It is highly desirable to develop a therapeutic, observable nanoparticle complex for specific targeting in cancer therapy. Growth hormone (GH) and its antagonists have been explored as cancer cell-targeting molecules for both imaging and therapeutic applications. In this study, a low toxicity, biocompatible, therapeutic, and observable GH-nanoparticle complex for specifically targeting growth hormone receptor (GHR) in cancer cells was synthesized by conjugating GH with green fluorescence protein and carboxylated nanodiamond. Moreover, we have shown that this complex can be triggered by laser irradiation to create a "nanoblast" and induce cell death in the A549 non-small-cell lung cancer cell line via the apoptotic pathway. This laser-mediated, cancer-targeting platform can be widely used in cancer therapy.
authors.affiliation: Department of Biological Science & Technology, National Chiao Tung, University, 75 Bo Ai Street, Hsinchu, Taiwan, 30068 (R.O.C.).
authors.forename: Hsueh-Liang
authors.initials: HL
authors.lastname: Chu
authors.forename: Hung-Wei
authors.initials: HW
authors.lastname: Chen
authors.forename: Shin-Hua
authors.initials: SH
authors.lastname: Tseng
authors.forename: Ming-Hua
authors.initials: MH
authors.lastname: Hsu
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Ho
authors.forename: Fu-Hsuan
authors.initials: FH
authors.lastname: Chou
authors.forename: Md Phd Hsing-Yuan
authors.initials: MP
authors.lastname: Li
authors.forename: Yu-Chuan
authors.initials: YC
authors.lastname: Chang
authors.forename: Pei-Hsin
authors.initials: PH
authors.lastname: Chen
authors.forename: Li-Yun
authors.initials: LY
authors.lastname: Tsai
authors.forename: Ching-Chung
authors.initials: CC
authors.lastname: Chou
authors.forename: Jyh Shin
authors.initials: JS
authors.lastname: Chen
authors.forename: Tsai-Mu
authors.initials: TM
authors.lastname: Cheng
authors.forename: Chia-Ching
authors.initials: CC
authors.lastname: Chang
mesh_terms: D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D049452:Green Fluorescent Proteins / Q000737:chemistry; D013006:Growth Hormone / Q000737:chemistry*; D006801:Humans; D007834:Lasers; D015394:Molecular Structure; D058612:Nanodiamonds / Q000737:chemistry*; D009369:Neoplasms / Q000188:drug therapy* / Q000473:pathology; D010316:Particle Size; D011986:Receptors, Somatotropin / Q000037:antagonists & inhibitors*; D013329:Structure-Activity Relationship; D013388:Succinimides / Q000737:chemistry; D013499:Surface Properties
chemical_list: D000970:Antineoplastic Agents; D001672:Biocompatible Materials; D058612:Nanodiamonds; D011986:Receptors, Somatotropin; D013388:Succinimides; D049452:Green Fluorescent Proteins; C035761:N-hydroxysulfosuccinimide; D013006:Growth Hormone
keywords: A549 lung cancer cells; apoptosis; lasers; nanodiamond complexes; recombinant growth hormones