title: FASN expression, angiogenesis and lymphangiogenesis in central and peripheral giant cell lesions.
abstract: Central giant cell lesion (CGCL) and peripheral giant cell lesion (PGCL) are non-neoplastic proliferative processes of the jaws. PGCL is a reactive process induced by irritant local factors and CGCL is an intra-osseous lesion of unknown etiology. Both lesions exhibit similar histologic features showing abundant mononuclear cells, admixed with a large number of multinucleated giant cells and a rich vascularized stroma with extravasated erythrocytes, hemosiderin deposition, and blood-filled pools. Recent studies have linked fatty acid synthase (FASN) with angiogenesis.
  
  
  OBJECTIVE
  To evaluate angiogenesis and lymphangiogenesis and their relationship with FASN expression in CGCL and PGCL.
  
  
  METHODS
  Thirteen CGCL and 14 PGCL of the jaws were selected for immunoexpression of FASN; CD34 and CD105 (to assess blood microvessel density [MVD] and microvessel area [MVA]); and D2-40 (to assess lymphatic MVD and MVA).
  
  
  RESULTS
  Within PGCL and CGCL, MVD-CD34 was signifcantly higher than MVD-CD10S, followed by MVD-D2-40. Moreover, a signifcantly higher number of FASN-positive multinucleated giant cells than mononuclear cells were observed. Between PGCL and CGCL, only MVD-CD34 and all MVA were signifcantly higher in PGCL. Positive correlation between MVA-CD10S with FASNpositive mononuclear cells in both lesions was observed.
  
  
  CONCLUSIONS
  Our results show both lesions exhibiting similar levels of FASN expression and neoangiogenesis, suggesting constitutive processes that regulate tissue maintenance.
authors.affiliation: Department of Dentistry, College of Basic Sciences and Health, Federal University of Vales do Jequitinhonha e Mucuri, Diamantina, MG, Brazil.
authors.forename: Saulo Gabriel Moreira
authors.initials: SG
authors.lastname: Falci
authors.affiliation: Department of Dentistry, College of Basic Sciences and Health, Federal University of Vales do Jequitinhonha e Mucuri, Diamantina, MG, Brazil.
authors.forename: Ana Terezinha Marques
authors.initials: AT
authors.lastname: Mesquita
authors.affiliation: School of Dentistry, Fluminense Federal University, Nova Friburgo, RJ, Brazil.
authors.forename: Bruno Augusto Benevenuto de
authors.initials: BA
authors.lastname: Andrade
authors.affiliation: Department of Dentistry, College of Basic Sciences and Health, Federal University of Vales do Jequitinhonha e Mucuri, Diamantina, MG, Brazil.
authors.forename: Joao Luiz de
authors.initials: JL
authors.lastname: Miranda
authors.affiliation: Department of Stomatology, Public Oral Health and Forensic Dentistry, University of Sao Paulo, Ribeirao Preto, SP, Brazil.
authors.forename: Jorge Esquiche
authors.initials: JE
authors.lastname: Leon
authors.affiliation: Oral Pathology Section, Department of Oral Diagnosis, Piracicaba Dental School, University of Campinas, Piracicaba, SP, Brazil.
authors.forename: Oslei Paes de
authors.initials: OP
authors.lastname: Almeida
authors.affiliation: Department of Dentistry, College of Basic Sciences and Health, Federal University of Vales do Jequitinhonha e Mucuri, Diamantina, MG, Brazil.
authors.forename: Cassio Roberto Rocha dos
authors.initials: CR
authors.lastname: Santos
mesh_terms: D000293:Adolescent; D000328:Adult; D015703:Antigens, CD / Q000032:analysis; D018952:Antigens, CD34 / Q000032:analysis; D001706:Biopsy; D000071063:Endoglin; D054890:Fatty Acid Synthase, Type I / Q000032:analysis*; D005260:Female; D015726:Giant Cells / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D007571:Jaw Diseases / Q000473:pathology*; D042583:Lymphangiogenesis / Q000502:physiology*; D008297:Male; D055806:Microvessels / Q000473:pathology; D009389:Neovascularization, Pathologic / Q000473:pathology*; D011956:Receptors, Cell Surface / Q000032:analysis; D012189:Retrospective Studies; D018709:Statistics, Nonparametric; D055815:Young Adult
chemical_list: D015703:Antigens, CD; D018952:Antigens, CD34; C064206:ENG protein, human; D000071063:Endoglin; D011956:Receptors, Cell Surface; C518729:FASN protein, human; D054890:Fatty Acid Synthase, Type I