title: Use of dried blood spots and inductively coupled plasma mass spectrometry for multi-element determination in blood.
abstract: The paper describes the development of an inductively coupled plasma mass spectrometry (ICP MS) method for multitrace element determination in dried blood spots (DBSs). The analytical conditions were optimized using Seronorm™ L-3 and L-1 Certified Reference Materials. The best results were obtained by sampling blood drops on a decontaminated PVDF filter membrane. After drying under metal-free conditions, the DBSs underwent acidic digestion and were analyzed with ICP MS. The method was then validated for As, Cd, Cu, Pb, Mo, Se and Zn. Using a matrix-matched calibration curve, the recovery levels ranged from 96% to 117%. The repeatability and reproducibility were generally below 15%. Limits of quantification ranging from 0.5 to 50 μg/L. In order to investigate the analytical procedure under real sampling conditions, the results obtained from DBSs and liquid blood aliquots (less subject to contamination) from two adult subjects were compared.
authors.affiliation: Ultra-Traces Analyses Aquitaine, Pau, France. Electronic address: veronique.vacchina@univ-pau.fr.
authors.forename: Véronique
authors.initials: V
authors.lastname: Vacchina
authors.affiliation: Newborn Screening Laboratory, Lille University Hospital, Lille, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Huin
authors.affiliation: EA 4483, University of Lille Nord de France, Lille, France.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Hulo
authors.affiliation: EA 4483, University of Lille Nord de France, Lille, France; Research in Industrial Environment Institute, University of Lille Nord de France, Lille, France.
authors.forename: Damien
authors.initials: D
authors.lastname: Cuny
authors.affiliation: EA 4483, University of Lille Nord de France, Lille, France.
authors.forename: Franck
authors.initials: F
authors.lastname: Broly
authors.affiliation: Newborn Screening Laboratory, Lille University Hospital, Lille, France.
authors.forename: Gilles
authors.initials: G
authors.lastname: Renom
authors.affiliation: Newborn Screening Laboratory, Lille University Hospital, Lille, France; EA 4483, University of Lille Nord de France, Lille, France; Research in Industrial Environment Institute, University of Lille Nord de France, Lille, France.
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Perini
mesh_terms: D001151:Arsenic / Q000097:blood; D001800:Blood Specimen Collection; D006801:Humans; D007854:Lead / Q000097:blood; D013058:Mass Spectrometry / Q000379:methods*; D014131:Trace Elements / Q000097:blood; D015032:Zinc / Q000097:blood
chemical_list: D014131:Trace Elements; D007854:Lead; D015032:Zinc; D001151:Arsenic
keywords: Blood; Dried blood spots; Inductively coupled plasma mass spectrometry; Multitrace element determination