title: Dual roles for the telomeric repeats in chromosomally integrated human herpesvirus-6.
abstract: Approximately 1 percent of healthy individuals carry human herpesvirus-6 within a host chromosome. This is referred to as chromosomally integrated herpesvirus-6 (CIHHV-6). In this study, we investigated the chromosomal integration site in six individuals harboring CIHHV-6B. Using FISH, we found that HHV-6B signals are consistently located at the telomeric region. The proximal endpoints of the integrated virus were mapped at one of two telomere-repeat-like sequences (TRSs) within the DR-R in all cases. In two cases, we isolated junction fragments between the viral TRS and human telomere repeats. The distal endpoints were mapped at the distal TRS in all cases. The size of the distal TRS was found to be ~5 kb which is sufficient to fulfill cellular telomeric functions. We conclude that the viral TRS in the DR regions fulfill dual functions for CIHHV-6: homology-mediated integration into the telomeric region of the chromosome and neo-telomere formation that is then stably transmitted.
authors.affiliation: Division of Molecular Genetics, Institute for Comprehensive Medical Science, Fujita Health University, Toyoake, Aichi 470-1192, Japan.
authors.forename: Tamae
authors.initials: T
authors.lastname: Ohye
authors.affiliation: Division of Molecular Genetics, Institute for Comprehensive Medical Science, Fujita Health University, Toyoake, Aichi 470-1192, Japan.
authors.forename: Hidehito
authors.initials: H
authors.lastname: Inagaki
authors.affiliation: 1] Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, Aichi 470-1192, Japan [2] Faculty of Clinical Engineering, Fujita Health University School of Health Sciences, Toyoake, Aichi 470-1192, Japan.
authors.forename: Masaru
authors.initials: M
authors.lastname: Ihira
authors.affiliation: 1] Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, Aichi 470-1192, Japan [2] Department of Laboratory Medicine, Fujita Health University Hospital, Toyoake, Aichi 470-1192, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Higashimoto
authors.affiliation: Department of Hematology and Oncology, Children's Medical Center, Japanese Red Cross Nagoya First Hospital, Nagoya, Aichi 453-8511, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Kato
authors.affiliation: Department of Pediatrics, Chiba University School of Medicine, Chiba, Chiba 260-8670, Japan.
authors.forename: Junko
authors.initials: J
authors.lastname: Oikawa
authors.affiliation: Department of Pediatrics, School of Medicine, Nihon University, Itabashi-ku, Tokyo 173-8610, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yagasaki
authors.affiliation: 1] Department of Pediatrics, Koshigaya Municipal Hospital, Koshigaya, Saitama 343-8577, Japan [2] Department of Pediatrics, Tokyo Rinkai Hospital, Edogawa-ku, Tokyo 134-0086, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Niizuma
authors.affiliation: Department of Pediatrics, Nagoya University Graduate School of Medicine, Nagoya, Aichi 466-8550, Japan.
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Takahashi
authors.affiliation: Department of Pediatrics, Nagoya University Graduate School of Medicine, Nagoya, Aichi 466-8550, Japan.
authors.forename: Seiji
authors.initials: S
authors.lastname: Kojima
authors.affiliation: Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, Aichi 470-1192, Japan.
authors.forename: Tetsushi
authors.initials: T
authors.lastname: Yoshikawa
authors.affiliation: Division of Molecular Genetics, Institute for Comprehensive Medical Science, Fujita Health University, Toyoake, Aichi 470-1192, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Kurahashi
mesh_terms: D001483:Base Sequence; D002875:Chromosomes / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics; D016679:Genome, Viral / Q000235:genetics; D015654:Herpesvirus 6, Human / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D016615:Telomere / Q000235:genetics*; D016662:Virus Integration / Q000235:genetics*
chemical_list: D004279:DNA, Viral