title: Promising antioxidant and anticancer (human breast cancer) oxidovanadium(IV) complex of chlorogenic acid. Synthesis, characterization and spectroscopic examination on the transport mechanism with bovine serum albumin.
abstract: A new chlorogenate oxidovanadium complex (Na[VO(chlorog)(H2O)3].4H2O) was synthesized by using Schlenk methodology in the course of a reaction at inert atmosphere in which deprotonated chlorogenic acid ligand binds to oxidovanadium(IV) in a reaction experiment controlled via EPR technique and based in a species distribution diagram. The compound was characterized by FTIR, EPR, UV-visible and diffuse reflectance spectroscopies and thermogravimetric, differential thermal and elemental analyses. The ligand and the complex were tested for their antioxidant effects on DPPH (1,1-diphenyl-2-picrylhydrazyl radical), ABTS(+) (radical cation of 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt), O2(-), OH and ROO radicals and their cytotoxic activity on different cancer cell lines (SKBR3, T47D and MDAMB231) and primary human mammary epithelial cells. The complex behaved as good antioxidant agent with strongest inhibitory effects on O2(-), OH and ROO radicals and exhibited selective cytotoxicity against SKBR3 cancer cell line. Albumin interaction experiments denoted high affinity toward the complex and its calculated binding constant was indicative of a strong binding to the protein. Based on this study, it is hypothesized that Na[VO(chlorog)(H2O)3].4H2O would be a promising candidate for further evaluation as an antioxidant and anticancer agent.
authors.affiliation: Centro de Química Inorgánica (CEQUINOR, CONICET, UNLP), Departamento de Química, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, 47 y 115, C.C. 962 (B1900AVV), 1900 La Plata, Argentina.
authors.forename: Luciana G
authors.initials: LG
authors.lastname: Naso
authors.affiliation: INNOPROT, Parque Tecnológico de Bizkaia, Edf. 502-1°, Derio, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Valcarcel
authors.affiliation: INNOPROT, Parque Tecnológico de Bizkaia, Edf. 502-1°, Derio, Spain.
authors.forename: Meritxell
authors.initials: M
authors.lastname: Roura-Ferrer
authors.affiliation: INNOPROT, Parque Tecnológico de Bizkaia, Edf. 502-1°, Derio, Spain.
authors.forename: Danel
authors.initials: D
authors.lastname: Kortazar
authors.affiliation: INNOPROT, Parque Tecnológico de Bizkaia, Edf. 502-1°, Derio, Spain.
authors.forename: Clarisa
authors.initials: C
authors.lastname: Salado
authors.affiliation: Departamento de Química Inorgánica, Facultad de Ciencia y Tecnología, Universidad del Pais Vasco, Apdo 644, 48080 Bilbao, Spain.
authors.forename: Luis
authors.initials: L
authors.lastname: Lezama
authors.affiliation: Departamento de Química Inorgánica, Facultad de Ciencia y Tecnología, Universidad del Pais Vasco, Apdo 644, 48080 Bilbao, Spain.
authors.forename: Teofilo
authors.initials: T
authors.lastname: Rojo
authors.affiliation: Centro de Química Inorgánica (CEQUINOR, CONICET, UNLP), Departamento de Química, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, 47 y 115, C.C. 962 (B1900AVV), 1900 La Plata, Argentina.
authors.forename: Ana C
authors.initials: AC
authors.lastname: González-Baró
authors.affiliation: Centro de Química Inorgánica (CEQUINOR, CONICET, UNLP), Departamento de Química, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, 47 y 115, C.C. 962 (B1900AVV), 1900 La Plata, Argentina.
authors.forename: Patricia A M
authors.initials: PA
authors.lastname: Williams
authors.affiliation: Centro de Química Inorgánica (CEQUINOR, CONICET, UNLP), Departamento de Química, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, 47 y 115, C.C. 962 (B1900AVV), 1900 La Plata, Argentina. Electronic address: evelina@quimica.unlp.edu.ar.
authors.forename: Evelina G
authors.initials: EG
authors.lastname: Ferrer
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000138:chemical synthesis* / Q000494:pharmacology; D001713:Biphenyl Compounds / Q000737:chemistry; D001943:Breast Neoplasms; D002417:Cattle; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival; D056831:Coordination Complexes / Q000138:chemical synthesis* / Q000494:pharmacology; D004355:Drug Stability; D005260:Female; D016166:Free Radical Scavengers / Q000138:chemical synthesis* / Q000494:pharmacology; D005609:Free Radicals / Q000737:chemistry; D006801:Humans; D010084:Oxidation-Reduction; D010851:Picrates / Q000737:chemistry; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013482:Superoxide Dismutase / Q000737:chemistry
chemical_list: D000970:Antineoplastic Agents; D001713:Biphenyl Compounds; D056831:Coordination Complexes; D016166:Free Radical Scavengers; D005609:Free Radicals; D010851:Picrates; D012710:Serum Albumin, Bovine; C004931:1,1-diphenyl-2-picrylhydrazyl; D013482:Superoxide Dismutase
keywords: Albumin interaction; Anticancer activity; Antioxidant activity; Chlorogenic acid; Oxidovanadium(IV)