title: Development of an allele-specific PCR for Escherichia coli B2 sub-typing, a rapid and easy to perform substitute of multilocus sequence typing.
abstract: We developed and validated an allele-specific PCR method for detecting the nine main Escherichia coli phylogroup B2 lineages involved in extra-intestinal infections, which could be used as a substitute for multilocus sequence typing in studies for which the greater resolution at the sequence type level is not needed.
authors.affiliation: INSERM, IAME, UMR 1137, F-75018 Paris, France; Univ Paris Diderot, IAME, UMR 1137, Sorbonne Paris Cité, F-75018 Paris, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Clermont
authors.affiliation: Division of Evolution, Ecology & Genetics, Research School of Biology, The Australian National University, Canberra ACT 0200, Australia.
authors.forename: Julia K
authors.initials: JK
authors.lastname: Christenson
authors.affiliation: INSERM, IAME, UMR 1137, F-75018 Paris, France; Univ Paris Nord, IAME, UMR 1137, Sorbonne Paris Cité, F-75018 Paris, France.
authors.forename: Anne-Sophie
authors.initials: AS
authors.lastname: Daubié
authors.affiliation: Division of Evolution, Ecology & Genetics, Research School of Biology, The Australian National University, Canberra ACT 0200, Australia.
authors.forename: David M
authors.initials: DM
authors.lastname: Gordon
authors.affiliation: INSERM, IAME, UMR 1137, F-75018 Paris, France; Univ Paris Diderot, IAME, UMR 1137, Sorbonne Paris Cité, F-75018 Paris, France. Electronic address: erick.denamur@inserm.fr.
authors.forename: Erick
authors.initials: E
authors.lastname: Denamur
mesh_terms: D000483:Alleles; D000818:Animals; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D004927:Escherichia coli Infections / Q000382:microbiology; D005798:Genes, Bacterial / Q000235:genetics*; D006801:Humans; D058885:Multilocus Sequence Typing / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D004269:DNA, Bacterial
keywords: Allele-specific PCR; B2 phylogroup; B2 sub-group; Escherichia coli; Sequence type complex