title: Near-infrared fluorescent 9-phenylethynylpyronin analogues for bioimaging.
abstract: The syntheses and biological applications of two novel fluorescent 9-phenylethynylpyronin analogues containing either carbon or silicon at the position 10 are reported. Both fluorescent probes exhibited a relatively strong fluorescence in methanol and phosphate buffer saline in the near-infrared region (705-738 nm) upon irradiation of either of their absorption maxima in the blue and red regions. The compounds showed high selectivity toward mitochondria in myeloma cells in vivo and allowed their visualization in a favored tissue-transparent window, which makes them promising NIR fluorescent tags for applications in bioimaging.
authors.affiliation: Department of Chemistry and ‡RECETOX, Faculty of Science, Masaryk University , Kamenice 5, 625 00 Brno, Czech Republic.
authors.forename: Tomáš
authors.initials: T
authors.lastname: Pastierik
authors.forename: Peter
authors.initials: P
authors.lastname: Sebej
authors.forename: Jiřina
authors.initials: J
authors.lastname: Medalová
authors.forename: Peter
authors.initials: P
authors.lastname: Stacko
authors.forename: Petr
authors.initials: P
authors.lastname: Klán
mesh_terms: D005456:Fluorescent Dyes / Q000737:chemistry*; D057054:Molecular Imaging; D053758:Nanoparticles / Q000737:chemistry*; D011754:Pyronine / Q000737:chemistry*; D019265:Spectroscopy, Near-Infrared
chemical_list: D005456:Fluorescent Dyes; D011754:Pyronine