title: Deep sequencing of pigeonpea sterility mosaic virus discloses five RNA segments related to emaraviruses.
abstract: The sequences of five viral RNA segments of pigeonpea sterility mosaic virus (PPSMV), the agent of sterility mosaic disease (SMD) of pigeonpea (Cajanus cajan, Fabaceae), were determined using the deep sequencing technology. Each of the five RNAs encodes a single protein on the negative-sense strand with an open reading frame (ORF) of 6885, 1947, 927, 1086, and 1,422 nts, respectively. In order, from RNA1 to RNA5, these ORFs encode the RNA-dependent RNA polymerase (p1, 267.9 kDa), a putative glycoprotein precursor (p2, 74.3 kDa), a putative nucleocapsid protein (p3, 34.6 kDa), a putative movement protein (p4, 40.8 kDa), while p5 (55 kDa) has an unknown function. All RNA segments of PPSMV showed the highest identity with orthologs of fig mosaic virus (FMV) and Rose rosette virus (RRV). In phylogenetic trees constructed with the amino acid sequences of p1, p2 and p3, PPSMV clustered consistently with other emaraviruses, close to clades comprising members of other genera of the family Bunyaviridae. Based on the molecular characteristics unveiled in this study and the morphological and epidemiological features similar to other emaraviruses, PPSMV seems to be the seventh species to join the list of emaraviruses known to date and accordingly, its classification in the genus Emaravirus seems now legitimate.
authors.affiliation: Istituto Agronomico Mediterraneo, Via Ceglie 9, 70010 Valenzano (BA), Italy. Electronic address: elbeaino@iamb.it.
authors.forename: Toufic
authors.initials: T
authors.lastname: Elbeaino
authors.affiliation: Istituto Agronomico Mediterraneo, Via Ceglie 9, 70010 Valenzano (BA), Italy.
authors.forename: Michele
authors.initials: M
authors.lastname: Digiaro
authors.affiliation: International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Hyderabad, Andhra Pradesh, India.
authors.forename: Mangala
authors.initials: M
authors.lastname: Uppala
authors.affiliation: International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Hyderabad, Andhra Pradesh, India.
authors.forename: Harikishan
authors.initials: H
authors.lastname: Sudini
mesh_terms: D036561:Cajanus / Q000821:virology*; D016000:Cluster Analysis; D059014:High-Throughput Nucleotide Sequencing; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D010942:Plant Viruses / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D012328:RNA Viruses / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D017385:Sequence Homology; D014764:Viral Proteins / Q000235:genetics
chemical_list: D014764:Viral Proteins
keywords: Deep-sequencing; Emaraviruses; Phylogenetic analyses; Pigeonpea sterility mosaic virus