title: Immunization with recombinant 3-1E protein in AbISCO®-300 adjuvant induced protective immunity against Eimeria acervulina infection in chickens.
abstract: Immunostimulating complexes (ISCOMs), a kind of novel antigen presenting system, could enhance immune protection by antigen presentation. AbISCO®-300 comprising purified saponin, cholesterol and phosphatidyl choline is an effective ISCOM adjuvant. To evaluate the immune protection of recombinant 3-1E protein against Eimeria acervulina infection, chickens were immunized with recombinant 3-1E protein in combination with AbISCO®-300 or recombinant 3-1E protein alone in this study. The protective immunity was assessed with body weight gain, fecal oocyst output, detection of intestinal IgA positive cells and percentages of CD3(+), CD4(+) or CD8(+) intestinal intraepithelial lymphocytes (IELs). Chickens vaccinated with different doses of recombinant 3-1E protein plus AbISCO®-300 showed higher percentages of CD3(+), CD4(+), and CD8(+) intestinal IELs, increased positive expression rate of intestinal IgA, increased body weight gains and decreased oocyst shedding compared with recombinant 3-1E protein-only vaccinated groups. The results showed that immunization with various doses of the recombinant 3-1E protein in AbISCO®-300 adjuvant enhanced immune protection against avian coccidiosis.
authors.affiliation: College of Animal Science and Technology, Beijing University of Agriculture, Beijing 102206, People's Republic of China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: School of Animal Science and Veterinary Medicine, Beijing Vocational College of Agriculture, Beijing 102442, People's Republic of China.
authors.forename: Lixia
authors.initials: L
authors.lastname: Wang
authors.affiliation: College of Animal Science and Technology, Beijing University of Agriculture, Beijing 102206, People's Republic of China.
authors.forename: Wenke
authors.initials: W
authors.lastname: Ruan
authors.affiliation: College of Animal Science and Technology, Beijing University of Agriculture, Beijing 102206, People's Republic of China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Beijing University of Agriculture, Beijing 102206, People's Republic of China.
authors.forename: Peng
authors.initials: P
authors.lastname: Yao
authors.affiliation: College of Animal Science and Technology, Beijing University of Agriculture, Beijing 102206, People's Republic of China.
authors.forename: Sai
authors.initials: S
authors.lastname: Zhou
authors.affiliation: College of Animal Science and Technology, Beijing University of Agriculture, Beijing 102206, People's Republic of China. Electronic address: anyh001@bac.edu.cn.
authors.forename: Jian
authors.initials: J
authors.lastname: An
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D018791:CD4 Lymphocyte Count / Q000662:veterinary; D018414:CD8-Positive T-Lymphocytes; D002645:Chickens / Q000469:parasitology*; D003048:Coccidiosis / Q000469:parasitology / Q000517:prevention & control / Q000662:veterinary*; D004539:Eimeria / Q000276:immunology*; D005243:Feces / Q000469:parasitology; D005434:Flow Cytometry / Q000662:veterinary; D007071:Immunoglobulin A, Secretory / Q000032:analysis; D007150:Immunohistochemistry / Q000662:veterinary; D007413:Intestinal Mucosa / Q000166:cytology / Q000276:immunology; D017730:Ki-1 Antigen / Q000097:blood; D018655:Lymphocyte Count / Q000662:veterinary; D008297:Male; D010270:Parasite Egg Count / Q000662:veterinary; D011201:Poultry Diseases / Q000469:parasitology / Q000517:prevention & control*; D015800:Protozoan Proteins / Q000235:genetics / Q000276:immunology*; D016052:Protozoan Vaccines; D011897:Random Allocation; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D015430:Weight Gain
chemical_list: D000276:Adjuvants, Immunologic; D007071:Immunoglobulin A, Secretory; D017730:Ki-1 Antigen; D015800:Protozoan Proteins; D016052:Protozoan Vaccines; D011994:Recombinant Proteins
keywords: Adjuvant; Chicken; Coccidiosis; Eimeria acervulina; Recombinant 3-1E protein