title: Accelerated cell-sheet recovery from a surface successively grafted with polyacrylamide and poly(N-isopropylacrylamide).
abstract: A double polymeric nanolayer consisting of poly(N-isopropylacrylamide) (PIPAAm) and hydrophilic polyacrylamide (PAAm) was deposited on tissue culture polystyrene (TCPS) surfaces using electron beam irradiation to form a new temperature-responsive cell culture surface in which the basal hydrophilic PAAm component in the double polymeric layer promotes the hydration of the upper PIPAAm layer and induces rapid cell detachment compared to a conventional temperature-responsive cell culture surface, PIPAAm-grafted TCPS (PIPAAm-TCPS). Take-off angle-dependent X-ray photoelectron spectroscopy spectral analysis demonstrated that the grafted PIPAAm and PAAm components were located in the upper and basal regions of the double polymeric layer, respectively, suggesting that the double polymeric layer forms an inter-penetrating-network-like structure with PAAm at the basal portion of the PIPAAm grafted chains. The wettability of the temperature-responsive cell culture surfaces with the double polymeric layer tended to be more hydrophilic, with an increase in the basal PAAm graft density at a constant PIPAAm graft density. However, when the graft densities of the upper PIPAAm and basal PAAm were optimized, the resulting temperature-responsive cell culture surface with the double polymeric layer exhibited rapid cell detachment while maintaining cell adhesive character comparable to that of PIPAAm-TCPS. The cell adhesive character was altered from cell-adhesive to cell-repellent with increasing PAAm or PIPAAm graft density. The cell adhesive character of the temperature-responsive cell culture surfaces was relatively consistent with their contact angles. These results strongly suggest that the basal PAAm surface properties affect the degree of hydration and dehydration of the subsequently grafted PIPAAm. In addition, the roles of the hydrophilic component in accelerating cell detachment are further discussed in terms of the mobility of the grafted PIPAAm chains. Applications of this insight might be useful for designing temperature-responsive cell culture surfaces for achieving efficient cell culture and quick target cell detachment.
authors.affiliation: Institute of Advanced Biomedical Engineering and Science and Global Center of Excellence (G-COE) Program, Tokyo Women's Medical University, 8-1 Kawadacho, Shinjuku-ku, Tokyo 162-8666, Japan.
authors.forename: Yoshikatsu
authors.initials: Y
authors.lastname: Akiyama
authors.affiliation: Institute of Advanced Biomedical Engineering and Science and Global Center of Excellence (G-COE) Program, Tokyo Women's Medical University, 8-1 Kawadacho, Shinjuku-ku, Tokyo 162-8666, Japan.
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kikuchi
authors.affiliation: Institute of Advanced Biomedical Engineering and Science and Global Center of Excellence (G-COE) Program, Tokyo Women's Medical University, 8-1 Kawadacho, Shinjuku-ku, Tokyo 162-8666, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Yamato
authors.affiliation: Institute of Advanced Biomedical Engineering and Science and Global Center of Excellence (G-COE) Program, Tokyo Women's Medical University, 8-1 Kawadacho, Shinjuku-ku, Tokyo 162-8666, Japan. Electronic address: tokano@abmes.twmu.ac.jp.
authors.forename: Teruo
authors.initials: T
authors.lastname: Okano
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D000327:Adsorption; D000818:Animals; D061249:Batch Cell Culture Techniques / Q000379:methods*; D001672:Biocompatible Materials / Q000737:chemistry; D002417:Cattle; D002448:Cell Adhesion / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology / Q000502:physiology*; D057927:Hydrophobic and Hydrophilic Interactions; D008422:Materials Testing; D008567:Membranes, Artificial; D011137:Polystyrenes / Q000737:chemistry*; D013499:Surface Properties; D013696:Temperature; D000099166:Polyacrylamides
chemical_list: D000180:Acrylic Resins; D001672:Biocompatible Materials; D008567:Membranes, Artificial; D011137:Polystyrenes; D000099166:Polyacrylamides; C052970:poly-N-isopropylacrylamide
keywords: Electron beam irradiation; Poly(N-isopropylacrylamide); Polyacrylamide; Ultra-thin