title: A novel lipid extraction method from wet microalga Picochlorum sp. at room temperature.
abstract: A novel method using ethanol was proposed for extracting lipids from wet microalga Picochlorum sp. at room temperature and pressure. In this study, Central Composite design (CCD) was applied to investigate the optimum conditions of lipid extraction. The results revealed that the solvent to biomass ratio had the largest effect on lipid extraction efficiency, followed by extraction time and temperature. A high lipid extraction yield (33.04% of the dry weight) was obtained under the following extraction conditions: 5 mL solvents per gram of wet biomass for 37 min with gentle stirring at room temperature. The extraction yield was comparable to that obtained by the widely used Bligh-Dyer method. Furthermore, no significant differences in the distribution of lipid classes and fatty acid composition were observed according to different extraction methods. In conclusion, these results indicated that the proposed procedure using ethanol could extract lipids from wet biomass efficiently and had giant potential for lipid extraction at large scale.
authors.affiliation: Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China. ycuyang@163.com.
authors.forename: Fangfang
authors.initials: F
authors.lastname: Yang
authors.affiliation: Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China. xwz@scsio.ac.cn.
authors.forename: Wenzhou
authors.initials: W
authors.lastname: Xiang
authors.affiliation: Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China. sunxm@scsio.ac.cn.
authors.forename: Xiumei
authors.initials: X
authors.lastname: Sun
authors.affiliation: Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China. hlwu@scsio.ac.cn.
authors.forename: Hualian
authors.initials: H
authors.lastname: Wu
authors.affiliation: Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China. taoli@scsio.ac.cn.
authors.forename: Tao
authors.initials: T
authors.lastname: Li
authors.affiliation: Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China. longlj@scsio.ac.cn.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Long
mesh_terms: D000465:Algorithms; D056804:Biofuels; D018533:Biomass; D003470:Culture Media; D004952:Esters / Q000737:chemistry; D000431:Ethanol; D005227:Fatty Acids / Q000032:analysis; D019156:Indian Ocean; D008055:Lipids / Q000737:chemistry* / Q000302:isolation & purification; D058086:Microalgae / Q000737:chemistry*; D008956:Models, Chemical; D015203:Reproducibility of Results; D012623:Seawater / Q000382:microbiology; D012997:Solvents; D013696:Temperature
chemical_list: D056804:Biofuels; D003470:Culture Media; D004952:Esters; D005227:Fatty Acids; D008055:Lipids; D012997:Solvents; D000431:Ethanol