title: Identification of novel sRNAs in Brucella abortus 2308.
abstract: Involved in diverse biological processes, bacterial sRNAs are novel regulators of gene expression involved in a wide array of biological processes. To identify sRNAs in Brucella abortus, we performed a genome-wide computational prediction with integrated SIPHT and NAPP results. In total, 129 sRNA candidates were identified, of which 112 were novel sRNA. Twenty novel sRNA candidates were tested by RT-PCR and seven could be verified. The putative targets of these sRNAs were also predicted and verified. This study provides a significant resource for the future study of sRNAs, as well as how sRNAs influence B. abortus physiology and pathogenesis.
authors.affiliation: Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Dong
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Peng
authors.forename: Ning
authors.initials: N
authors.lastname: Wang
authors.forename: Qingmin
authors.initials: Q
authors.lastname: Wu
mesh_terms: D002003:Brucella abortus / Q000235:genetics*; D019295:Computational Biology; D012329:RNA, Bacterial / Q000235:genetics*; D058727:RNA, Small Untranslated / Q000235:genetics*
chemical_list: D012329:RNA, Bacterial; D058727:RNA, Small Untranslated
keywords: Brucella; sRNA; sRNA targets