title: Hexane Fractions of Bupleurum falcatum L. Stimulates Glucagon-Like Peptide-1 Secretion through G β γ -Mediated Pathway.
abstract: Bupleurum falcatum L. has been used traditionally as a medicinal herb in Korean medicine. The hexane fraction of BF (HFBF), which was profiled with Direct Analysis in Real Time-Mass Spectrometry (DART-MS), activates the secretion of glucagon-like peptide-1 (GLP-1) in NCI-H716 cells significantly. We performed a microarray analysis and GLP-1 ELISA assay, as well as calcium imaging experiments with inhibitors, to investigate the mechanism of action of the HFBF. Through the microarray analysis, it was found that the ITPR2 gene that encodes the inositol 1,4,5-trisphosphate (IP3) receptor is up-regulated and the HFBF induces cell depolarization by inhibiting the voltage-gated channel expression in NCI-H716 cells. In addition, we found that the intracellular calcium in NCI-H716 cells, with Gallein, U73122, and 2APB as inhibitors, was decreased. These results suggest that the HFBF activates the GLP-1 secretion through the G βγ pathways in the enteroendocrine L cells after treatment with the HFBF.
authors.affiliation: College of Korean Medicine, Institute of Korean Medicine, Kyung Hee University, 1 Heogi-dong, Dongdaemun-gu, Seoul 130-701, Republic of Korea.
authors.forename: Min-Hee
authors.initials: MH
authors.lastname: Shin
authors.affiliation: College of Korean Medicine, Institute of Korean Medicine, Kyung Hee University, 1 Heogi-dong, Dongdaemun-gu, Seoul 130-701, Republic of Korea.
authors.forename: Eun-Kyeong
authors.initials: EK
authors.lastname: Choi
authors.affiliation: College of Korean Medicine, Institute of Korean Medicine, Kyung Hee University, 1 Heogi-dong, Dongdaemun-gu, Seoul 130-701, Republic of Korea.
authors.forename: Ki-Suk
authors.initials: KS
authors.lastname: Kim
authors.affiliation: College of Korean Medicine, Institute of Korean Medicine, Kyung Hee University, 1 Heogi-dong, Dongdaemun-gu, Seoul 130-701, Republic of Korea.
authors.forename: Kang-Hoon
authors.initials: KH
authors.lastname: Kim
authors.affiliation: College of Pharmacy, Kyung Hee University, Heogi-dong, Dongdaemun-gu, Seoul 130-701, Republic of Korea.
authors.forename: Young Pyo
authors.initials: YP
authors.lastname: Jang
authors.affiliation: College of Korean Medicine, Institute of Korean Medicine, Kyung Hee University, 1 Heogi-dong, Dongdaemun-gu, Seoul 130-701, Republic of Korea.
authors.forename: Kwang Seok
authors.initials: KS
authors.lastname: Ahn
authors.affiliation: College of Korean Medicine, Institute of Korean Medicine, Kyung Hee University, 1 Heogi-dong, Dongdaemun-gu, Seoul 130-701, Republic of Korea.
authors.forename: Won-Seok
authors.initials: WS
authors.lastname: Chung
authors.affiliation: Nursing Department, College of Health Science, Kangwon University, Hyoja 2-dong, Chuncheon-si 200-701, Republic of Korea.
authors.forename: Nam Hyun
authors.initials: NH
authors.lastname: Cha
authors.affiliation: College of Korean Medicine, Institute of Korean Medicine, Kyung Hee University, 1 Heogi-dong, Dongdaemun-gu, Seoul 130-701, Republic of Korea.
authors.forename: Hyeung-Jin
authors.initials: HJ
authors.lastname: Jang