title: Screening for voltage-gated sodium channel interacting peptides.
abstract: The voltage-gated sodium channel (VGSC) interacting peptide is of special interest for both basic research and pharmaceutical purposes. In this study, we established a yeast-two-hybrid based strategy to detect the interaction(s) between neurotoxic peptide and the extracellular region of VGSC. Using a previously reported neurotoxin JZTX-III as a model molecule, we demonstrated that the interactions between JZTX-III and the extracellular regions of its target hNav1.5 are detectable and the detected interactions are directly related to its activity. We further applied this strategy to the screening of VGSC interacting peptides. Using the extracellular region of hNav1.5 as the bait, we identified a novel sodium channel inhibitor SSCM-1 from a random peptide library. This peptide selectively inhibits hNav1.5 currents in the whole-cell patch clamp assays. This strategy might be used for the large scale screening for target-specific interacting peptides of VGSCs or other ion channels.
authors.affiliation: 1] State Key Laboratory of High Performance Computing, Research Center of Biological Information, National University of Defense Technology, Changsha, Hunan 410073, China [2].
authors.forename: Er
authors.initials: E
authors.lastname: Meng
authors.affiliation: 1] Key Laboratory of Protein Chemistry and Developmental Biology of the Ministry of Education, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, China [2].
authors.forename: Tian-Fu
authors.initials: TF
authors.lastname: Cai
authors.affiliation: Key Laboratory of Protein Chemistry and Developmental Biology of the Ministry of Education, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Protein Chemistry and Developmental Biology of the Ministry of Education, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, China.
authors.forename: Si
authors.initials: S
authors.lastname: Tang
authors.affiliation: Key Laboratory of Protein Chemistry and Developmental Biology of the Ministry of Education, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, China.
authors.forename: Meng-Jie
authors.initials: MJ
authors.lastname: Li
authors.affiliation: State Key Laboratory of High Performance Computing, Research Center of Biological Information, National University of Defense Technology, Changsha, Hunan 410073, China.
authors.forename: Wen-Ying
authors.initials: WY
authors.lastname: Li
authors.affiliation: Key Laboratory of Protein Chemistry and Developmental Biology of the Ministry of Education, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, China.
authors.forename: Peng-Fei
authors.initials: PF
authors.lastname: Huang
authors.affiliation: Key Laboratory of Protein Chemistry and Developmental Biology of the Ministry of Education, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Liu
authors.affiliation: State Key Laboratory of High Performance Computing, Research Center of Biological Information, National University of Defense Technology, Changsha, Hunan 410073, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wu
authors.affiliation: State Key Laboratory of High Performance Computing, Research Center of Biological Information, National University of Defense Technology, Changsha, Hunan 410073, China.
authors.forename: Ling-Yun
authors.initials: LY
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of High Performance Computing, Research Center of Biological Information, National University of Defense Technology, Changsha, Hunan 410073, China.
authors.forename: Long
authors.initials: L
authors.lastname: Liu
authors.affiliation: Core Facilities of Biotechnology, Central South University of Forestry and Technology, Changsha, Hunan 410004, China.
authors.forename: Kuan
authors.initials: K
authors.lastname: Peng
authors.affiliation: Beijing Institute of Pharmaceutical Chemistry, Beijing 102205, China.
authors.forename: Xian-Dong
authors.initials: XD
authors.lastname: Dai
authors.affiliation: Beijing Institute of Pharmaceutical Chemistry, Beijing 102205, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Key Laboratory of Protein Chemistry and Developmental Biology of the Ministry of Education, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, China.
authors.forename: Xiong-Zhi
authors.initials: XZ
authors.lastname: Zeng
authors.affiliation: Key Laboratory of Protein Chemistry and Developmental Biology of the Ministry of Education, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, China.
authors.forename: Song-Ping
authors.initials: SP
authors.lastname: Liang
authors.affiliation: State Key Laboratory of High Performance Computing, Research Center of Biological Information, National University of Defense Technology, Changsha, Hunan 410073, China.
authors.forename: Dong-Yi
authors.initials: DY
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D005109:Extracellular Matrix / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D049990:Membrane Transport Modulators / Q000378:metabolism*; D008969:Molecular Sequence Data; D009498:Neurotoxins / Q000378:metabolism; D010455:Peptides / Q000378:metabolism*; D061566:Voltage-Gated Sodium Channels / Q000378:metabolism*
chemical_list: D049990:Membrane Transport Modulators; D009498:Neurotoxins; D010455:Peptides; D061566:Voltage-Gated Sodium Channels