title: New sesquiterpenic acids from Inula wissmanniana.
abstract: Eight new (1-8) and two known (9 and 10) sesquiterpenic acids featuring α-methylene-γ-carboxyl units were isolated from the whole plants of Inula wissmanniana, along with two new germacranolides (11 and 12). Their structures were elucidated based on detailed spectroscopic analysis, including HRESIMS, 1D and 2D NMR, and X-ray crystallography. Notably, the skeleton of 1 was firstly discovered from nature, while that of 2 was discovered for the second time. All the compounds were evaluated for their inhibition against LPS-induced nitric oxide (NO) production in RAW264.7 macrophages. Compound 11 exhibited the strongest activity with the IC50 value of 1.04 μM.
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, PR China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu Province, PR China.
authors.forename: Xiang-Rong
authors.initials: XR
authors.lastname: Cheng
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, PR China.
authors.forename: Chun-Hui
authors.initials: CH
authors.lastname: Wang
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, PR China.
authors.forename: Pan-Lei
authors.initials: PL
authors.lastname: Wei
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, PR China.
authors.forename: Xu-Feng
authors.initials: XF
authors.lastname: Zhang
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, PR China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zeng
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, PR China.
authors.forename: Shi-Kai
authors.initials: SK
authors.lastname: Yan
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, PR China. Electronic address: kimhz@sjtu.edu.cn.
authors.forename: Hui-Zi
authors.initials: HZ
authors.lastname: Jin
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, PR China; School of Pharmacy, Second Military Medical University, Shanghai 200433, PR China. Electronic address: wdzhangy@hotmail.com.
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Zhang
mesh_terms: D000818:Animals; D018360:Crystallography, X-Ray; D020128:Inhibitory Concentration 50; D038143:Inula / Q000737:chemistry*; D008070:Lipopolysaccharides / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D008516:Medicine, Chinese Traditional; D051379:Mice; D015394:Molecular Structure; D009569:Nitric Oxide / Q000378:metabolism*; D035261:Plant Components, Aerial / Q000737:chemistry; D010946:Plants, Medicinal; D012717:Sesquiterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D045788:Sesquiterpenes, Germacrane / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D008070:Lipopolysaccharides; D012717:Sesquiterpenes; D045788:Sesquiterpenes, Germacrane; C432449:germacranolide; D009569:Nitric Oxide
keywords: Asteraceae; Inula wissmanniana; NO production; RAW264.7 macrophages; Sesquiterpenic acids