title: [Feasibility of monitoring clopidogrel resistance with flow cytometric analysis of platelet vasodilator stimulated phosphoprotein phosphorylation].
abstract: OBJECTIVE
  To evaluate the feasibility of monitoring clopidogrel resistance with flow cytometric analysis of platelet vasodilator stimulated phosphoprotein (VASP) phosphorylation.
  
  
  METHODS
  Twenty patients with coronary heart disease (CHD) and 17 healthy volunteers were examined for platelet aggregation rate and phosphorylation of VASP (calculated as platelet reactivity index, PRI) using traditional optical nephelometry and flow cytometry before and after concurrent therapy of clopidogrel and aspirin.
  
  
  RESULTS
  No significant differences were found in PRI between CHD group and healthy control group [(89.45∓5.22)% vs (86.58∓4.35)%] before treatment. The PRI in CHD group was significantly lowered after treatment to (67.66∓19.77)% (P<0.05). Clopidogrel resistance was found in 6 (30%) cases in CHD group by flow cytometric analysis, which showed a higher sensitivity than optical nephelometry (10%).
  
  
  CONCLUSIONS
  Flow cytometric analysis of VASP phosphorylation is a more reliable test to specifically evaluate clopidogrel resistance.
authors.affiliation: Guangdong General Hospital/ Guangdong Academy of Medical Science, Guangzhou 510080 , China. E-mail: liguanghua2014@139.com.
authors.forename: Guanghua
authors.initials: G
authors.lastname: Li
authors.forename: Yanfei
authors.initials: Y
authors.lastname: Luo
authors.forename: Ying
authors.initials: Y
authors.lastname: Luo
authors.forename: Ting
authors.initials: T
authors.lastname: Lin
authors.forename: Xiaobin
authors.initials: X
authors.lastname: Fan
authors.forename: Ling
authors.initials: L
authors.lastname: Liang
authors.forename: Hongdong
authors.initials: H
authors.lastname: Xie
authors.forename: Jingwei
authors.initials: J
authors.lastname: Huang
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D015815:Cell Adhesion Molecules / Q000737:chemistry*; D000077144:Clopidogrel; D004351:Drug Resistance; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008840:Microfilament Proteins / Q000737:chemistry*; D008875:Middle Aged; D010750:Phosphoproteins / Q000737:chemistry*; D010766:Phosphorylation; D010979:Platelet Function Tests; D013988:Ticlopidine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000098662:Vasodilator-Stimulated Phosphoprotein
chemical_list: D015815:Cell Adhesion Molecules; D008840:Microfilament Proteins; D010750:Phosphoproteins; D000098662:Vasodilator-Stimulated Phosphoprotein; D000077144:Clopidogrel; D013988:Ticlopidine