title: cDNA cloning and expression characterization of serum transferrin gene from oriental weatherfish Misgurnus anguillicaudatus.
abstract: In this study, the cDNA coding serum transferrin (stf) of Misgurnus anguillicaudatus (mastf) was cloned. mastf cDNA is composed of 2326 bp with a 2007 bp open reading frame encoding 668 amino acids. The deduced Mastf protein consists of a signal peptide, two lobes (N and C-lobes) and signature motifs of transferrin (Tf) family. The results of tissue distribution indicated that mastf mRNA was predominantly expressed in the liver. The results indicate that the mastf expression increased significantly in liver, blood, spleen and head kidney after the challenge with Aeromonas sobria, acting as a positive acute protein, suggesting that mastf is related to the immune response. The cloning and expression analysis of mastf further demonstrates the evolutionary conservation of Stf and immune function in vertebrates.
authors.affiliation: Jiangsu Key Laboratory of Marine Biotechnology, Huaihai Institute of Technology, Lianyungang 222005, China; Key Laboratory of East China Sea & Oceanic Fishery Resources Exploitation and Utilization, Ministry of Agriculture, Shanghai 200090, China; Jiangsu Province R & D Institute of Marine Resources, Huaihai Institute of Technology, Lianyungang 222005, China.
authors.forename: J H
authors.initials: JH
authors.lastname: Chen
authors.forename: C H
authors.initials: CH
authors.lastname: Wang
authors.forename: Y L
authors.initials: YL
authors.lastname: Li
authors.forename: H M
authors.initials: HM
authors.lastname: Wang
authors.forename: X J
authors.initials: XJ
authors.lastname: Zhang
authors.forename: B L
authors.initials: BL
authors.lastname: Yan
mesh_terms: D000333:Aeromonas; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003531:Cypriniformes / Q000097:blood / Q000235:genetics* / Q000276:immunology; D018076:DNA, Complementary / Q000235:genetics; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism*; D060226:Head Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D012333:RNA, Messenger / Q000378:metabolism; D017422:Sequence Analysis, DNA; D013154:Spleen / Q000378:metabolism; D014168:Transferrin / Q000235:genetics / Q000378:metabolism*
chemical_list: D018076:DNA, Complementary; D029941:Fish Proteins; D012333:RNA, Messenger; D014168:Transferrin
keywords: Aeromonas sobria; Cypriniformes; gene expression; immune response; infection; real-time RT-PCR