title: Lidocaine effect on flotillin-2 distribution in detergent-resistant membranes of equine jejunal smooth muscle in vitro.
abstract: Lidocaine is the most commonly chosen prokinetic for treating postoperative ileus in horses, a motility disorder associated with ischaemia-reperfusion injury of intestinal tissues. Despite the frequent use of lidocaine, the mechanism underlying its prokinetic effects is still unclear. Previous studies suggested that lidocaine altered cell membrane characteristics of smooth muscle cells. Therefore, the present study aimed to elucidate effects of lidocaine administration on characteristics of detergent-resistant membranes in equine jejunal smooth muscle. Lidocaine administration caused significant redistribution of flotillin-2, a protein marker of detergent-resistant membranes, in fractions of sucrose-density-gradients obtained from ischaemia-reperfusion injured smooth muscle solubilised with Triton X-100. It was concluded that lidocaine induced disruption of detergent-resistant membranes which might affect ion channel activity and therefore enhance smooth muscle contractility.
authors.affiliation: Department of Physiology, University of Veterinary Medicine, Bischofsholer Damm 15, 30173 Hannover, Germany.
authors.forename: Karen
authors.initials: K
authors.lastname: Tappenbeck
authors.affiliation: Department of Physiological Chemistry, University of Veterinary Medicine, Bünteweg 17, 30559 Hannover, Germany.
authors.forename: Sonja
authors.initials: S
authors.lastname: Schmidt
authors.affiliation: Clinic for Horses, University of Veterinary Medicine, Bünteweg 9, 30559 Hannover, Germany.
authors.forename: Karsten
authors.initials: K
authors.lastname: Feige
authors.affiliation: Department of Physiological Chemistry, University of Veterinary Medicine, Bünteweg 17, 30559 Hannover, Germany.
authors.forename: Hassan Y
authors.initials: HY
authors.lastname: Naim
authors.affiliation: Department of Physiology, University of Veterinary Medicine, Bischofsholer Damm 15, 30173 Hannover, Germany. Electronic address: korinna.huber@tiho-hannover.de.
authors.forename: Korinna
authors.initials: K
authors.lastname: Huber
mesh_terms: D000818:Animals; D000889:Anti-Arrhythmia Agents / Q000494:pharmacology*; D015415:Biomarkers / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects* / Q000378:metabolism; D006736:Horses / Q000378:metabolism*; D007583:Jejunum / Q000187:drug effects / Q000378:metabolism; D008012:Lidocaine / Q000494:pharmacology*; D008565:Membrane Proteins / Q000378:metabolism*; D009130:Muscle, Smooth / Q000187:drug effects* / Q000378:metabolism
chemical_list: D000889:Anti-Arrhythmia Agents; D015415:Biomarkers; D008565:Membrane Proteins; C106354:flotillins; D008012:Lidocaine
keywords: Contractility; Detergent; Flotillin; Lidocaine; Lipid rafts