title: Non-specific phospholipase C5 and diacylglycerol promote lateral root development under mild salt stress in Arabidopsis.
abstract: Developing a robust root system is crucial to plant survival and competition for soil resources. Here we report that the non-specific phospholipase C5 (NPC5) and its derived lipid mediator diacylglycerol (DAG) mediate lateral root (LR) development during salt stress in Arabidopsis thaliana. T-DNA knockout mutant npc5-1 produced few to no LR under mild NaCl stress, whereas overexpression of NPC5 increased LR number. Roots of npc5-1 contained a lower level of DAG than wild type, whereas NPC5 overexpressor exhibited an increase in DAG level. Application of DAG, but not phosphatidic acid, fully restored LR growth of npc5-1 to that of wild type under NaCl stress. NPC5 expression was significantly induced in Arabidopsis seedlings treated with NaCl. Npc5-1 was less responsive to auxin-mediated root growth than the wild type. These results indicate that NPC5 mediates LR development in response to salt stress and suggest that DAG functions as a lipid mediator in the stress signalling.
authors.affiliation: Department of Biology, University of Missouri, St. Louis, MO, 63121, USA; Donald Danforth Plant Science Center, St. Louis, MO, 63132, USA.
authors.forename: Carlotta
authors.initials: C
authors.lastname: Peters
authors.forename: Sang-Chul
authors.initials: SC
authors.lastname: Kim
authors.forename: Shivakumar
authors.initials: S
authors.lastname: Devaiah
authors.forename: Maoyin
authors.initials: M
authors.lastname: Li
authors.forename: Xuemin
authors.initials: X
authors.lastname: Wang
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D004075:Diglycerides / Q000096:biosynthesis / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D055786:Gene Knockout Techniques; D007210:Indoleacetic Acids / Q000494:pharmacology; D009154:Mutation / Q000235:genetics; D010740:Phospholipases / Q000235:genetics / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development*; D021381:Protein Transport; D012965:Sodium Chloride / Q000494:pharmacology*; D013312:Stress, Physiological / Q000187:drug effects* / Q000235:genetics; D013347:Subcellular Fractions / Q000201:enzymology; D010738:Type C Phospholipases / Q000235:genetics / Q000378:metabolism*
chemical_list: C011439:1,2-diacylglycerol; D029681:Arabidopsis Proteins; D004075:Diglycerides; D007210:Indoleacetic Acids; D010743:Phospholipids; D012965:Sodium Chloride; C030737:indoleacetic acid; D010740:Phospholipases; C000596153:NPC5 protein, Arabidopsis; D010738:Type C Phospholipases
keywords: lateral roots; lipid signalling; phospholipase