title: Bradyrhizobium paxllaeri sp. nov. and Bradyrhizobium icense sp. nov., nitrogen-fixing rhizobial symbionts of Lima bean (Phaseolus lunatus L.) in Peru.
abstract: A group of strains isolated from root nodules of Phaseolus lunatus (Lima bean) in Peru were characterized by genotypic, genomic and phenotypic methods. All strains possessed identical 16S rRNA gene sequences that were 99.9% identical to that of Bradyrhizobium lablabi CCBAU 23086(T). Despite having identical 16S rRNA gene sequences, the Phaseolus lunatus strains could be divided into two clades by sequence analysis of recA, atpD, glnII, dnaK and gyrB genes. The genome sequence of a representative of each clade was obtained and compared to the genomes of closely related species of the genus Bradyrhizobium. Average nucleotide identity values below the species circumscription threshold were obtained when comparing the two clades to each other (88.6%) and with all type strains of the genus Bradyrhizobium (≤92.9%). Phenotypes distinguishing both clades from all described and closely related species of the genus Bradyrhizobium were found. On the basis of the results obtained, two novel species, Bradyrhizobium paxllaeri sp. nov. (type strain LMTR 21(T) = DSM 18454(T) = HAMBI 2911(T)) and Bradyrhizobium icense sp. nov. (type strain LMTR 13(T) = HAMBI 3584(T) = CECT 8509(T) = CNPSo 2583(T)), are proposed to accommodate the uncovered clades of Phaseolus lunatus bradyrhizobia. These species share highly related but distinct nifH and nodC symbiosis genes.
authors.affiliation: Departamento de Biotecnología (ETS de Ingenieros Agrónomos) and Centro de Biotecnología y Genómica de Plantas (CBGP), Universidad Politécnica de Madrid, Spain.
authors.forename: David
authors.initials: D
authors.lastname: Durán
authors.affiliation: Departamento de Biotecnología (ETS de Ingenieros Agrónomos) and Centro de Biotecnología y Genómica de Plantas (CBGP), Universidad Politécnica de Madrid, Spain.
authors.forename: Luis
authors.initials: L
authors.lastname: Rey
authors.affiliation: Laboratorio de Ecología Microbiana y Biotecnología 'Marino Tabusso', Universidad Nacional Agraria La Molina, Peru.
authors.forename: Juan
authors.initials: J
authors.lastname: Mayo
authors.affiliation: Laboratorio de Ecología Microbiana y Biotecnología 'Marino Tabusso', Universidad Nacional Agraria La Molina, Peru.
authors.forename: Doris
authors.initials: D
authors.lastname: Zúñiga-Dávila
authors.affiliation: Departamento de Biotecnología (ETS de Ingenieros Agrónomos) and Centro de Biotecnología y Genómica de Plantas (CBGP), Universidad Politécnica de Madrid, Spain.
authors.forename: Juan
authors.initials: J
authors.lastname: Imperial
authors.affiliation: Departamento de Biotecnología (ETS de Ingenieros Agrónomos) and Centro de Biotecnología y Genómica de Plantas (CBGP), Universidad Politécnica de Madrid, Spain.
authors.forename: Tomás
authors.initials: T
authors.lastname: Ruiz-Argüeso
authors.affiliation: Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Mexico.
authors.forename: Esperanza
authors.initials: E
authors.lastname: Martínez-Romero
authors.affiliation: Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Mexico.
authors.forename: Ernesto
authors.initials: E
authors.lastname: Ormeño-Orrillo
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D020369:Bradyrhizobium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009586:Nitrogen Fixation; D009693:Nucleic Acid Hybridization; D010568:Peru; D027805:Phaseolus / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D053204:Root Nodules, Plant / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D013559:Symbiosis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S