title: One-dimensional SDS-polyacrylamide gel electrophoresis (1D SDS-PAGE).
abstract: This protocol describes a denaturing polyacrylamide gel system utilizing sodium dodecyl sulfate (SDS) to separate protein molecules based on size as first described by Laemmli (1970). SDS-PAGE can be used to monitor protein purifications, check the purity of samples, and to estimate molecular weights for unknown proteins.
authors.affiliation: Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USA; Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
authors.forename: Julie L
authors.initials: JL
authors.lastname: Brunelle
authors.affiliation: Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USA; Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD, USA. Electronic address: ragreen@jhmi.edu.
authors.forename: Rachel
authors.initials: R
authors.lastname: Green
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel / Q000295:instrumentation / Q000379:methods*; D011506:Proteins / Q000032:analysis* / Q000302:isolation & purification
chemical_list: D011506:Proteins
keywords: Ammonium persulfate (APS); One-dimensional SDS-Polyacrylamide Gel Electrophoresis (1D SDS-PAGE); Polyacrylamide; Polyacrylamide gel electrophoresis; SDS-PAGE gel; Stacking gel; Tetramethylethylenediamine (TEMED)