title: Galanthamine, an anticholinesterase drug, effects plant growth and development in Artemisia tridentate Nutt. via modulation of auxin and neutrotransmitter signaling.
abstract: Galanthamine is a naturally occurring acetylcholinesterase (AchE) inhibitor that has been well established as a drug for treatment of mild to moderate Alzheimer disease, but the role of the compound in plant metabolism is not known. The current study was designed to investigate whether galanthamine could redirect morphogenesis of Artemisia tridentata Nutt. cultures by altering concentration of endogenous neurosignaling molecules acetylcholine (Ach), auxin (IAA), melatonin (Mel), and serotonin (5HT). Exposure of axenic A. tridentata cultures to 10 µM galanthamine decreased the concentration of endogenous Ach, IAA, MEL, and AchE, and altered plant growth in a manner reminiscent of 2-4D toxicity. Galanthamine itself demonstrated IAA activity in an oat coleotile elongation bioassay, 20 µM galanthamine showed no significant difference compared with 5 μM IAA or 5 μM 1-Naphthaleneacetic acid (NAA). Metabolomic analysis detected between 20,921 to 27,891 compounds in A. tridentata plantlets and showed greater commonality between control and 5 µM treatments. Furthermore, metabolomic analysis putatively identified coumarins scopoletin/isoscopoletin, and scopolin in A. tridentata leaf extracts and these metabolites linearly increased in response to galanthamine treatments. Overall, these data indicate that galanthamine is an allelopathic phytochemical and support the hypothesis that neurologically active compounds in plants help ensure plant survival and adaptation to environmental challenges.
authors.affiliation: Biology; University of British Columbia; Okanagan Campus; Kelowna, BC Canada.
authors.forename: Christina E
authors.initials: CE
authors.lastname: Turi
authors.affiliation: Chemistry; University of British Columbia; Okanagan Campus; Kelowna, BC Canada.
authors.forename: Katarina E
authors.initials: KE
authors.lastname: Axwik
authors.affiliation: Chemistry; University of British Columbia; Okanagan Campus; Kelowna, BC Canada.
authors.forename: Anderson
authors.initials: A
authors.lastname: Smith
authors.affiliation: Department of Plant Agriculture; University of Guelph; Guelph, ON Canada.
authors.forename: A Maxwell P
authors.initials: AM
authors.lastname: Jones
authors.affiliation: Department of Plant Agriculture; University of Guelph; Guelph, ON Canada.
authors.forename: Praveen K
authors.initials: PK
authors.lastname: Saxena
authors.affiliation: Chemistry; University of British Columbia; Okanagan Campus; Kelowna, BC Canada.
authors.forename: Susan J
authors.initials: SJ
authors.lastname: Murch
mesh_terms: D000109:Acetylcholine / Q000378:metabolism; D000110:Acetylcholinesterase / Q000378:metabolism; D019306:Artemisia / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D002800:Cholinesterase Inhibitors / Q000494:pharmacology*; D046508:Culture Techniques; D005702:Galantamine / Q000494:pharmacology*; D007210:Indoleacetic Acids / Q000378:metabolism*; D008550:Melatonin / Q000378:metabolism; D018384:Oxidative Stress; D063245:Plant Development / Q000187:drug effects*; D012701:Serotonin / Q000378:metabolism
chemical_list: D002800:Cholinesterase Inhibitors; D007210:Indoleacetic Acids; D005702:Galantamine; D012701:Serotonin; D000110:Acetylcholinesterase; D008550:Melatonin; D000109:Acetylcholine
keywords: Acetylcholine; Artemisia tridentata; Auxin; Galanthamine; Melatonin; cholinergic signalling; metabolomics