title: Hymenobacter kanuolensis sp. nov., a novel radiation-resistant bacterium.
abstract: A Gram-reaction-negative, rod-shaped, non-motile, red-pigmented, radiation-resistant, aerobic bacterium designated T-3(T) was isolated from a soil sample from the Qinghai-Tibet Plateau in Tibet, China, after exposure to 10 kGy gamma radiation. Phylogenetic analysis based on 16S rRNA sequences indicated that this isolate represented a novel member of the genus Hymenobacter. Sequence identities of the 16S rRNA gene of strain T-3(T) with the type strains of species of the genus Hymenobacter with validly published names range from 89% to 97%, and the most closely related species is Hymenobacter psychrotolerans Tibet-IIU11(T) (97%). The DNA-DNA relatedness between strain T-3(T) and H. psychrotolerans is 59.10%. The major fatty acids of strain T-3(T) were iso-C(15 : 0) (27.66%), summed feature 4 (iso-C(17 : 1)I and/or anteiso-C(17 : 1)B, 15.84%), anteiso-C(15 : 0) (14.08%) and summed feature 3 (C(16 : 1)ω7c and/or C(16 : 1)ω6c, 12.38%). The major menaquinone of strain T-3(T) was MK-7. Phosphatidylethanolamine (PE) was predominant in the polar lipid profile. The G+C content of the DNA of strain T-3(T) was 69.17 mol%. On the basis of the results of the polyphasic characterization presented in this study, it is concluded that strain T-3(T) represents a novel species of the genus Hymenobacter, for which the name Hymenobacter kanuolensis is proposed. The type strain is T-3(T) ( = ACCC 05760(T) = KCTC 32407(T)).
authors.affiliation: Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
authors.forename: Shiyou
authors.initials: S
authors.lastname: Su
authors.affiliation: Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
authors.forename: Ming
authors.initials: M
authors.lastname: Chen
authors.affiliation: Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
authors.forename: Chao
authors.initials: C
authors.lastname: Teng
authors.affiliation: Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
authors.forename: Shijie
authors.initials: S
authors.lastname: Jiang
authors.affiliation: Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
authors.forename: Chen
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Lin
authors.affiliation: Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D041964:Cytophagaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000528:radiation effects*; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005720:Gamma Rays; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D011836:Radiation Tolerance; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C483858:phosphatidylethanolamine; C062629:menaquinone 7