title: Biomimetic microfluidic device for in vitro antihypertensive drug evaluation.
abstract: Microfluidic devices have emerged as revolutionary, novel platforms for in vitro drug evaluation. In this work, we developed a facile method for evaluating antihypertensive drugs using a microfluidic chip. This microfluidic chip was generated using the elastic material poly(dimethylsiloxane) (PDMS) and a microchannel structure that simulated a blood vessel as fabricated on the chip. We then cultured human umbilical vein endothelial cells (HUVECs) inside the channel. Different pressures and shear stresses could be applied on the cells. The generated vessel mimics can be used for evaluating the safety and effects of antihypertensive drugs. Here, we used hydralazine hydrochloride as a model drug. The results indicated that hydralazine hydrochloride effectively decreased the pressure-induced dysfunction of endothelial cells. This work demonstrates that our microfluidic system provides a convenient and cost-effective platform for studying cellular responses to drugs under mechanical pressure.
authors.affiliation: Key Laboratory of Cryogenics, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences & Beijing Key Laboratory of Cryo-Biomedical Engineering , Beijing100190, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Lv
authors.forename: Serge
authors.initials: S
authors.lastname: Ostrovidov
authors.forename: Xuetao
authors.initials: X
authors.lastname: Shi
authors.forename: Ning
authors.initials: N
authors.lastname: Zhang
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
mesh_terms: D000959:Antihypertensive Agents / Q000737:chemistry / Q000494:pharmacology*; D040761:Biomimetic Materials / Q000737:chemistry / Q000494:pharmacology*; D032701:Biomimetics / Q000379:methods*; D001808:Blood Vessels / Q000187:drug effects; D002478:Cells, Cultured; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D004341:Drug Evaluation / Q000379:methods; D042783:Endothelial Cells / Q000187:drug effects; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D006830:Hydralazine / Q000737:chemistry / Q000494:pharmacology; D066298:In Vitro Techniques / Q000379:methods; D056656:Lab-On-A-Chip Devices / Q000379:methods*; D044085:Microfluidics / Q000379:methods; D009757:Nylons / Q000737:chemistry; D011312:Pressure; D033081:Shear Strength / Q000187:drug effects; D013314:Stress, Mechanical
chemical_list: D000959:Antihypertensive Agents; D004129:Dimethylpolysiloxanes; D009757:Nylons; C085707:poly(dimethylsiloxane)-polyamide copolymer; D006830:Hydralazine