title: TET2 overexpression in chronic lymphocytic leukemia is unrelated to the presence of TET2 variations.
abstract: TET2 is involved in a variety of hematopoietic malignancies, mainly in myeloid malignancies. Most mutations of TET2 have been identified in myeloid disorders, but some have also recently been described in mature lymphoid neoplasms. In contrast to the large amount of data about mutations of TET2, some data are available for gene expression. Moreover, the role of TET2 in chronic lymphocytic leukemia (CLL) is unknown. This study analyzes both TET2 expression and mutations in 48 CLL patients. TET2 expression was analyzed by exon arrays and quantitative real-time polymerase chain reaction (qRT-PCR). Next-generation sequencing (NGS) technology was applied to investigate the presence of TET2 variations. Overexpression of TET2 was observed in B-cell lymphocytes from CLL patients compared with healthy donors (P = 0.004). In addition, in CLL patients, an overexpression of TET2 was also observed in the clonal B cells compared with the nontumoral cells (P = 0.002). However, no novel mutations were observed. Therefore, overexpression of TET2 in CLL seems to be unrelated to the presence of genomic TET2 variations.
authors.affiliation: IBSAL, IBMCC, Centro de Investigación del Cáncer, Universidad de Salamanca-CSIC, 37007 Salamanca, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Hernández-Sánchez
authors.affiliation: IBSAL, IBMCC, Centro de Investigación del Cáncer, Universidad de Salamanca-CSIC, 37007 Salamanca, Spain.
authors.forename: Ana Eugenia
authors.initials: AE
authors.lastname: Rodríguez
authors.affiliation: Department of Molecular Genetics, MLL Munich Leukemia Laboratory, 81377 Munich, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Kohlmann
authors.affiliation: IBSAL, IBMCC, Centro de Investigación del Cáncer, Universidad de Salamanca-CSIC, 37007 Salamanca, Spain.
authors.forename: Rocío
authors.initials: R
authors.lastname: Benito
authors.affiliation: Instituto de Estudios de Ciencias de la Salud de Castilla y León (IECSCYL) and Hospital Clínico Universitario de Salamanca (HUSAL), 37007 Castilla y León, Spain.
authors.forename: Juan Luis
authors.initials: JL
authors.lastname: García
authors.affiliation: Celgene Institute for Translational Research Europe (CITRE), 41092 Sevilla, Spain ; Unidad de Bioinformática y Genómica Funcional, IBMCC, Centro de Investigación del Cáncer, Universidad de Salamanca-CSIC, 37007 Salamanca, Spain.
authors.forename: Alberto
authors.initials: A
authors.lastname: Risueño
authors.affiliation: Unidad de Genómica, IBMCC, Centro de Investigación del Cáncer, Universidad de Salamanca-CSIC, 37007 Salamanca, Spain.
authors.forename: Encarna
authors.initials: E
authors.lastname: Fermiñán
authors.affiliation: Unidad de Bioinformática y Genómica Funcional, IBMCC, Centro de Investigación del Cáncer, Universidad de Salamanca-CSIC, 37007 Salamanca, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: De Las Rivas
authors.affiliation: IBSAL, IBMCC, Centro de Investigación del Cáncer, Universidad de Salamanca-CSIC, 37007 Salamanca, Spain ; Servicio de Hematología y Departamento de Medicina, Hospital Clínico Universitario de Salamanca, Paseo San Vicente 58, 37007 Salamanca, Spain.
authors.forename: Marcos
authors.initials: M
authors.lastname: González
authors.affiliation: IBSAL, IBMCC, Centro de Investigación del Cáncer, Universidad de Salamanca-CSIC, 37007 Salamanca, Spain ; Servicio de Hematología y Departamento de Medicina, Hospital Clínico Universitario de Salamanca, Paseo San Vicente 58, 37007 Salamanca, Spain.
authors.forename: Jesús-María
authors.initials: JM
authors.lastname: Hernández-Rivas
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001402:B-Lymphocytes / Q000378:metabolism; D016022:Case-Control Studies; D002999:Clone Cells; D004268:DNA-Binding Proteins / Q000235:genetics*; D049308:Dioxygenases; D005260:Female; D015973:Gene Expression Regulation, Leukemic; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009154:Mutation / Q000235:genetics*; D011518:Proto-Oncogene Proteins / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; D011518:Proto-Oncogene Proteins; D049308:Dioxygenases; C541081:TET2 protein, human