title: Dynamical structure of αB-crystallin.
abstract: The human small heat-shock protein αB-crystallin is an extremely difficult molecule to study, with its inherent structural dynamics posing unique challenges to all biophysical and structural biology techniques. Here we highlight how the polydispersity and quaternary dynamics of αB-crystallin are intrinsically inter-twined, and how this can impact on measurements of the oligomeric distribution. We show that, in spite of these difficulties, considerable understanding of the varied fluctuations αB-crystallin undergoes at equilibrium has emerged in the last few years. By reporting on data obtained from a variety of biophysical techniques, we demonstrate how the αB-crystallin solution ensemble is governed by molecular motions of varying amplitude and time-scales spanning several orders of magnitude. We describe how these diverse measurements are being used to construct an integrated view of the dynamical structure of αB-crystallin, and highlight areas that require further interrogation. With its study motivating the refinement of experimental techniques, and the development of new approaches to combine the hybrid datasets, we conclude that αB-crystallin continues to represent a paradigm for dynamical biology.
authors.affiliation: Department of Chemistry, Physical and Theoretical Chemistry Laboratory, University of Oxford, South Parks Road, Oxford OX1 3QZ, United Kingdom.
authors.forename: Georg K A
authors.initials: GK
authors.lastname: Hochberg
authors.affiliation: Department of Chemistry, Physical and Theoretical Chemistry Laboratory, University of Oxford, South Parks Road, Oxford OX1 3QZ, United Kingdom. Electronic address: justin.benesch@chem.ox.ac.uk.
authors.forename: Justin L P
authors.initials: JL
authors.lastname: Benesch
mesh_terms: D000494:Allosteric Regulation; D000818:Animals; D006801:Humans; D007700:Kinetics; D055503:Protein Multimerization; D020836:Protein Structure, Quaternary; D038203:alpha-Crystallin B Chain / Q000737:chemistry* / Q000378:metabolism
chemical_list: D038203:alpha-Crystallin B Chain
keywords: CRYAB; HSPB5; Molecular chaperone; Protein dynamics; Small heat-shock protein; αB-crystallin