title: Pigment epithelium-derived factor protects cone photoreceptor-derived 661W cells from light damage through Akt activation.
abstract: Pigment epithelium-derived factor (PEDF) can delay and prevent the death of photoreceptors in vivo. We investigated the survival activity of PEDF on cone photoreceptor-derived 661W cells in culture, the presence of PEDF receptor (PEDF-R) in these cells and the activation of prosurvival Akt. Cell death was induced by light exposure in the presence of 9-cis retinal. Cell viability assays showed that PEDF increased the number of 661W cells exposed to these conditions. Western blots showed that PEDF-treated 661W cells had a higher ratio of phosphorylated Akt to total Akt than untreated cells. The PEDF receptor PEDF-R was immunodetected in the plasma membrane fractions of 661W cells. The results demonstrated that PEDF can protect 661W cells against light-induced cell death and suggest that the binding of PEDF to cell surface PEDF-R triggers a prosurvival signaling pathway.
authors.affiliation: National Eye Institute, National Institutes of Health, Bldg. 6, Rm. 131F, 6 Center Dr., MSC 0608, 20892-0608, Bethesda, MD, USA, rappm1@umbc.edu.
authors.forename: Matthew
authors.initials: M
authors.lastname: Rapp
authors.forename: Grace
authors.initials: G
authors.lastname: Woo
authors.forename: Muayyad R
authors.initials: MR
authors.lastname: Al-Ubaidi
authors.forename: S Patricia
authors.initials: SP
authors.lastname: Becerra
authors.forename: Preeti
authors.initials: P
authors.lastname: Subramanian
mesh_terms: D000818:Animals; D016923:Cell Death / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D005136:Eye Proteins / Q000378:metabolism* / Q000494:pharmacology; D008027:Light / Q000009:adverse effects*; D051379:Mice; D009414:Nerve Growth Factors / Q000378:metabolism* / Q000494:pharmacology; D018696:Neuroprotective Agents / Q000378:metabolism* / Q000494:pharmacology; D010766:Phosphorylation / Q000502:physiology; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D018013:Receptors, Neuropeptide / Q000378:metabolism; D017949:Retinal Cone Photoreceptor Cells / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D015843:Serpins / Q000378:metabolism* / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology; D000099174:Pigment Epithelium-Derived Factor
chemical_list: D005136:Eye Proteins; D009414:Nerve Growth Factors; D018696:Neuroprotective Agents; D051057:Proto-Oncogene Proteins c-akt; D018013:Receptors, Neuropeptide; D015843:Serpins; D000099174:Pigment Epithelium-Derived Factor; C400531:pigment epithelium-derived factor receptor; C514862:Akt1 protein, mouse