title: Intestinal autophagy activity is essential for host defense against Salmonella typhimurium infection in Caenorhabditis elegans.
abstract: Salmonella typhimurium infects both intestinal epithelial cells and macrophages. Autophagy is a lysosomal degradation pathway that is present in all eukaryotes. Autophagy has been reported to limit the Salmonella replication in Caenorhabditis elegans and in mammals. However, it is unknown whether intestinal autophagy activity plays a role in host defense against Salmonella infection in C. elegans. In this study, we inhibited the autophagy gene bec-1 in different C. elegans tissues and examined the survival of these animals following Salmonella infection. Here we show that inhibition of the bec-1 gene in the intestine but not in other tissues confers susceptibility to Salmonella infection, which is consistent with recent studies in mice showing that autophagy is involved in clearance of Salmonella in the intestinal epithelial cells. Therefore, the intestinal autophagy activity is essential for host defense against Salmonella infection from C. elegans to mice, perhaps also in humans.
authors.affiliation: Department of Biological Sciences, Florida Atlantic University, Jupiter, FL, USA.
authors.forename: Alexander
authors.initials: A
authors.lastname: Curt
authors.affiliation: Department of Biological Sciences, Florida Atlantic University, Jupiter, FL, USA.
authors.forename: Jiuli
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Biological Sciences, Florida Atlantic University, Jupiter, FL, USA.
authors.forename: Justin
authors.initials: J
authors.lastname: Minnerly
authors.affiliation: Department of Biological Sciences, Florida Atlantic University, Jupiter, FL, USA. Electronic address: kjia@fau.edu.
authors.forename: Kailiang
authors.initials: K
authors.lastname: Jia
mesh_terms: D000818:Animals; D001343:Autophagy; D017173:Caenorhabditis elegans / Q000276:immunology* / Q000382:microbiology*; D004195:Disease Models, Animal; D006801:Humans; D007422:Intestines / Q000166:cytology / Q000276:immunology / Q000382:microbiology; D051379:Mice; D012480:Salmonella Infections / Q000276:immunology; D012486:Salmonella typhimurium / Q000502:physiology*
keywords: Autophagy; C. elegans; Pathogen-host interaction; Salmonella; bec-1