title: Morphological and electrophysiological features of mature neurons in differentiated skin-derived precursor cells.
abstract: In vitro modelling of neuronal pathologies is, in particular, demanding and a lot of efforts have been undertaken to differentiate skin derived precursor cells into neuronal cells. However, so far all attempts did not result in cells with functional features of neurons like the ability to generate action potentials or synaptic activity. Here, we report that simple modifications of the protocols result in neuronal cells that display action potentials and synaptic activity. We think that our observation is an important step to model individual neuronal pathologies in vitro.
authors.affiliation: Institute of Human Genetics , ; Institute of Clinical Chemistry .
authors.forename: L
authors.initials: L
authors.lastname: Liebmann
authors.affiliation: Institute of Clinical Chemistry .
authors.forename: C
authors.initials: C
authors.lastname: Beetz
authors.affiliation: Department of Oral, Maxillofacial and Plastic Surgery, University Hospital Jena, Friedrich Schiller University , Jena, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Thorwarth
authors.affiliation: Institute of Clinical Chemistry .
authors.forename: T
authors.initials: T
authors.lastname: Deufel
authors.affiliation: Institute of Human Genetics , ; Institute of Clinical Chemistry .
authors.forename: Ca
authors.initials: C
authors.lastname: Hübner
keywords: neuronal differentiation; skin-derived precursors; stem cells