title: Characterization of St-Valerien-like virus genome detected in Japan.
abstract: A novel calicivirus, St-Valerien-like virus (SVV), has been identified in asymptomatic swine in Canada, Italy and the U.S.A. In this study, we characterized a new SVV strain (NUP-24/JP) detected in fecal samples of swine in Japan. The NUP-24/JP genome had 6,409 nucleotides and 2 open reading frames (ORF1 and ORF2). ORF1 and ORF2 consist of 5,940 and 453 nucleotides, respectively. Phylogenetic analysis revealed that NUP-24/JP was closely related to other SVV strains, particularly to U.S.A. strain NC-WGS93C/US. This finding suggests that SVV is prevalent in swine worldwide. Using a baculovirus expression system, we successfully produced virus-like particles, which would be useful for seroepidemiological studies of SVV.
authors.affiliation: Laboratory of Veterinary Microbiology, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan.
authors.forename: Go
authors.initials: G
authors.lastname: Sato
authors.forename: Hisayuki
authors.initials: H
authors.lastname: Ido
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kiuchi
authors.forename: Michiyo
authors.initials: M
authors.lastname: Kataoka
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Katayama
authors.forename: Yukinobu
authors.initials: Y
authors.lastname: Tohya
mesh_terms: D000818:Animals; D002139:Caliciviridae / Q000235:genetics* / Q000302:isolation & purification*; D017250:Caliciviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D016679:Genome, Viral; D007564:Japan / Q000453:epidemiology; D010802:Phylogeny; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology / Q000821:virology*