title: Evaluation of Mdh1 protein as an antigenic candidate for a vaccine against candidiasis.
abstract: Candida albicans malate dehydrogenase (Mdh1p) has been screened by previous proteome studies as a candidate for a vaccine against candidiasis. In this study, recombinant Mdh1 protein with a His-tag was produced in Escherichia coli and evaluated as an immunogenic protein against candidiasis. Mdh1p was administrated to mice by two methods subcutaneous injection and intranasal administration before challenging them with a lethal dose of C. albicans. After vaccination of Mdh1p, antibody responses were observed. To evaluate the vaccination effect of Mdh1p, survival tests were performed after 35 d. Although all control mice died within 24 d or 25 d, 100% and 80% of mice survived with subcutaneous and intranasal administration, respectively. Therefore, our results indicate that, among C. albicans antigens examined thus far, Mdh1p is currently the most effective antigen for use as a vaccine for C. albicans.
authors.affiliation: General Education Center, Hyogo University of Health Sciences.
authors.forename: Seiji
authors.initials: S
authors.lastname: Shibasaki
authors.forename: Wataru
authors.initials: W
authors.lastname: Aoki
authors.forename: Takashi
authors.initials: T
authors.lastname: Nomura
authors.forename: Miki
authors.initials: M
authors.lastname: Karasaki
authors.forename: Tomomitsu
authors.initials: T
authors.lastname: Sewaki
authors.forename: Mitsuyoshi
authors.initials: M
authors.lastname: Ueda
mesh_terms: D000818:Animals; D000908:Antibodies, Fungal / Q000276:immunology; D002176:Candida albicans / Q000201:enzymology* / Q000235:genetics / Q000276:immunology; D002177:Candidiasis / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D004353:Drug Evaluation, Preclinical; D005260:Female; D005656:Fungal Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D005657:Fungal Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D006801:Humans; D008291:Malate Dehydrogenase / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D014611:Vaccination
chemical_list: D000908:Antibodies, Fungal; D005656:Fungal Proteins; D005657:Fungal Vaccines; D008291:Malate Dehydrogenase