title: A rapid, site-selective and efficient route to the dual modification of DARPins.
abstract: Designed ankyrin repeat proteins (DARPins) are valuable tools in both biochemistry and medicine. Herein we describe a rapid, simple method for the dual modification of DARPins by introduction of cysteine mutations at specific positions that results in a vast difference in their thiol nucleophilicity, allowing for clean sequential modification.
authors.affiliation: Department of Chemistry, University College London, 20 Gordon Street, London, WC1H 0AJ, UK. vpenterprise@ucl.ac.uk.
authors.forename: Paul
authors.initials: P
authors.lastname: Moody
authors.forename: Vijay
authors.initials: V
authors.lastname: Chudasama
authors.forename: Ramiz I
authors.initials: RI
authors.lastname: Nathani
authors.forename: Antoine
authors.initials: A
authors.lastname: Maruani
authors.forename: Stephen
authors.initials: S
authors.lastname: Martin
authors.forename: Mark E B
authors.initials: ME
authors.lastname: Smith
authors.forename: Stephen
authors.initials: S
authors.lastname: Caddick
mesh_terms: D017487:Ankyrins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002942:Circular Dichroism; D003545:Cysteine / Q000378:metabolism; D006801:Humans; D016297:Mutagenesis, Site-Directed; D011485:Protein Binding; D015202:Protein Engineering; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000737:chemistry / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics
chemical_list: D017487:Ankyrins; D003545:Cysteine; D018719:Erb-b2 Receptor Tyrosine Kinases; D011994:Recombinant Proteins