title: Sequence variability in the structural protein-encoding region of foot-and-mouth disease virus serotype A and O of Ethiopian isolates.
abstract: A total of 13 serotype O and 5 serotype A FMD Ethiopian isolates and some isolates from other countries (six for serotype A and four for serotype O) were sequenced on the structural protein (P1) coding region. The deduced amino acid sequences were aligned and investigated in an attempt to determine the amino acid variation. Differences were observed at 115 (15.6%) and 119 (16.1%) amino acid positions for serotype O and serotype A, respectively. The variation in the derived amino acid sequences is the highest in VP1, while VP4 was highly conserved in both serotypes A and O. In all isolates, hypervariable regions were located at regions corresponding to the highly immunogenic sites, the G-H loop (133-158) and the C-terminus (194-213) of the VP1 gene. The RGD cell attachment site within the G-H loop of the gene was conserved in all isolates. The study revealed the presence of significant amino acid variation at VP2 and VP3 in addition to known VP1 coding region. Hence, determination of amino acid sequence of the whole P1 region provides more information on antigenic variability of FMD virus and could be used in vaccine strain selection in parallel with serological vaccine matching assays.
authors.affiliation: National Veterinary Institute, P. O. Box 19, Hora street, Debre-zeit, Ethiopia; Department of Food Safety and Infection Biology, Norwegian School of Veterinary Science, Centre for Epidemiology and Biostatistics, P.O. Box 8146, 0033 Oslo, Norway. Electronic address: gelagayayelet@yahoo.com.
authors.forename: Gelagay
authors.initials: G
authors.lastname: Ayelet
authors.affiliation: National Veterinary Institute, P. O. Box 19, Hora street, Debre-zeit, Ethiopia.
authors.forename: Esayas
authors.initials: E
authors.lastname: Gelaye
authors.affiliation: National Veterinary Institute, P. O. Box 19, Hora street, Debre-zeit, Ethiopia.
authors.forename: Shiferaw
authors.initials: S
authors.lastname: Jenberie
authors.affiliation: Department of Food Safety and Infection Biology, Norwegian School of Veterinary Science, Centre for Epidemiology and Biostatistics, P.O. Box 8146, 0033 Oslo, Norway; School of Veterinary Medicine, Hawassa University, P.O. Box 05, Hawassa, Ethiopia.
authors.forename: Kassahun
authors.initials: K
authors.lastname: Asmare
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000940:Antigenic Variation / Q000235:genetics*; D001483:Base Sequence; D005002:Ethiopia; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012313:RNA / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D015678:Viral Structural Proteins / Q000235:genetics*
chemical_list: D015678:Viral Structural Proteins; D012313:RNA
keywords: Amino acid variability; Ethiopia; FMD virus; Serotype O and A; Structural protein genome