title: Mir-23b and miR-130b expression is downregulated in pituitary adenomas.
abstract: MicroRNA (miRNA) deregulation plays a critical role in tumorigenesis. miR-23b and miR-130b are induced by thyrotropin in thyroid cells in a cAMP-dependent manner. The aim of our work has been to investigate the possible role of miR-23b and miR-130b in pituitary tumorigenesis. We have analyzed their expression in a panel of pituitary adenomas (PAs) including GH and NFPA adenomas. We report that miR-23b and miR-130b are drastically reduced in GH, gonadotroph and NFPA adenomas in comparison with normal pituitary gland. Interestingly, the overexpression of miR-23b and miR-130b inhibits cell proliferation arresting the cells in the G1 and G2 phase of the cell cycle, respectively. Moreover, we demonstrate that miR-23b and miR-130b target HMGA2 and cyclin A2 (CCNA2) genes, respectively. Finally, downregulation of miR-23b and miR-130b expression is associated with increased levels of their respective targets in human PAs. These findings suggest that miR-23b and miR-130b downregulation may contribute to pituitary tumorigenesis.
authors.affiliation: Istituto di Endocrinologia ed Oncologia Sperimentale del CNR, c/o Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Scuola di Medicina e Chirurgia di Napoli, Università degli Studi di Napoli "Federico II", Naples, Italy.
authors.forename: Vincenza
authors.initials: V
authors.lastname: Leone
authors.affiliation: Istituto di Endocrinologia ed Oncologia Sperimentale del CNR, c/o Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Scuola di Medicina e Chirurgia di Napoli, Università degli Studi di Napoli "Federico II", Naples, Italy.
authors.forename: Concetta
authors.initials: C
authors.lastname: Langella
authors.affiliation: Istituto di Endocrinologia ed Oncologia Sperimentale del CNR, c/o Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Scuola di Medicina e Chirurgia di Napoli, Università degli Studi di Napoli "Federico II", Naples, Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: D'Angelo
authors.affiliation: Istituto di Endocrinologia ed Oncologia Sperimentale del CNR, c/o Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Scuola di Medicina e Chirurgia di Napoli, Università degli Studi di Napoli "Federico II", Naples, Italy.
authors.forename: Paula
authors.initials: P
authors.lastname: Mussnich
authors.affiliation: INSERM U1052, Centre de Recherche en Cancerologie de Lyon, F-69000 Lyon, France and University Lyon, F-69000 Lyon, France; Profilexpert UNIV-US7 INSERM-UMS 3453 CNRS Lyon, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Wierinckx
authors.affiliation: Molecular Pathology Division Institute of Pathology, University HospitalSchönbeinstrasse, 40CH-4003 Basel, Switzerland.
authors.forename: Luigi
authors.initials: L
authors.lastname: Terracciano
authors.affiliation: INSERM U1028, CNRS UMR 5292, Lyon Neuroscience Research Center, Neuro-oncology and Neuroinflammation Team, F-69372 Lyon Cedex 08, France and University Lyon1, F-69000 Lyon, France.
authors.forename: Gerald
authors.initials: G
authors.lastname: Raverot
authors.affiliation: Profilexpert UNIV-US7 INSERM-UMS 3453 CNRS Lyon, France.
authors.forename: Joel
authors.initials: J
authors.lastname: Lachuer
authors.affiliation: Department ofClinical Applied Sciencesand Biotechnology, University of L'Aquila Via Vetoio, Coppito 2, 67100 L'Aquila, Italy.
authors.forename: Sandra
authors.initials: S
authors.lastname: Rotondi
authors.affiliation: Department ofClinical Applied Sciencesand Biotechnology, University of L'Aquila Via Vetoio, Coppito 2, 67100 L'Aquila, Italy; Neuromed, IRCCS, Pozzilli, Italy.
authors.forename: Marie-Lise
authors.initials: ML
authors.lastname: Jaffrain-Rea
authors.affiliation: INSERM U1052, Centre de Recherche en Cancerologie de Lyon, F-69000 Lyon, France and University Lyon, F-69000 Lyon, France; INSERM U1028, CNRS UMR 5292, Lyon Neuroscience Research Center, Neuro-oncology and Neuroinflammation Team, F-69372 Lyon Cedex 08, France and University Lyon1, F-69000 Lyon, France.
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Trouillas
authors.affiliation: Istituto di Endocrinologia ed Oncologia Sperimentale del CNR, c/o Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Scuola di Medicina e Chirurgia di Napoli, Università degli Studi di Napoli "Federico II", Naples, Italy. Electronic address: alfusco@unina.it.
authors.forename: Alfredo
authors.initials: A
authors.lastname: Fusco
mesh_terms: D020413:3' Untranslated Regions; D000236:Adenoma / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D063646:Carcinogenesis / Q000235:genetics / Q000378:metabolism; D016022:Case-Control Studies; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D057809:HEK293 Cells; D025743:HMGA2 Protein / Q000235:genetics / Q000378:metabolism; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D010902:Pituitary Gland / Q000378:metabolism / Q000473:pathology; D010911:Pituitary Neoplasms / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D051381:Rats
chemical_list: D020413:3' Untranslated Regions; D025743:HMGA2 Protein; C554019:MIRN130 microRNA, human; C552430:MIRN23a microRNA, human; D035683:MicroRNAs
keywords: CCNA2; HMGA2; Pituitary adenoma; microRNA