title: A novel JK null allele associated with typing discrepancies among African Americans.
abstract: The Jknun (Jk-3) phenotype, attributable to null or silenced alleles, has predominantly been found in persons of Polynesian descent. With the increased use of molecular genotyping, many new silencing mutations have been identified in persons of other ethnic backgrounds. To date, only two JK null alleles have been reported in African Americans, JK*01N.04 and JK*OlN.OS.A comparative study was undertaken to determine whether JK mutations were present in the regional African American population. Results of donor genotyping were compared with previously recorded results of serologic tests, and discrepant results were investigated. Although the two previously identified polymorphisms were not detected in the discrepant samples, a novel allele (191G>A) was identified and was assigned the ISBT number JK*02N.09. This study illustrates a limitation of using single-nucleotide polymorphisms for prediction of blood group antigens.
authors.affiliation: MT(ASCP)SBB (corresponding author), Supervisor, Clinical Immunogenetics, Scientific Support Services, LifeShare Blood Centers, 8910 Linwood Avenue, Shreveport, LA 71106;
authors.forename: Katrina L
authors.initials: KL
authors.lastname: Billingsley
authors.affiliation: BS, Research Technologist II, Puget Sound Blood Center, Seattle, WA.
authors.forename: Jeff B
authors.initials: JB
authors.lastname: Posadas
authors.affiliation: PhD, MT(ASCP)SBB, Director, Scientific Support Services, LifeShare Blood Centers, Shreveport, LA.
authors.forename: Joann M
authors.initials: JM
authors.lastname: Moulds
authors.affiliation: PhD, Senior Scientist, Puget Sound Blood Center, Seattle, WA.
authors.forename: Lakshmi K
authors.initials: LK
authors.lastname: Gaur
mesh_terms: D001741:Black or African American / Q000235:genetics*; D000483:Alleles; D060005:Genotyping Techniques; D006801:Humans; D007667:Kidd Blood-Group System / Q000235:genetics* / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide; D012189:Retrospective Studies
chemical_list: D007667:Kidd Blood-Group System