title: Tumor lysing genetically engineered T cells loaded with multi-modal imaging agents.
abstract: Genetically-modified T cells expressing chimeric antigen receptors (CAR) exert anti-tumor effect by identifying tumor-associated antigen (TAA), independent of major histocompatibility complex. For maximal efficacy and safety of adoptively transferred cells, imaging their biodistribution is critical. This will determine if cells home to the tumor and assist in moderating cell dose. Here, T cells are modified to express CAR. An efficient, non-toxic process with potential for cGMP compliance is developed for loading high cell number with multi-modal (PET-MRI) contrast agents (Super Paramagnetic Iron Oxide Nanoparticles - Copper-64; SPION-(64)Cu). This can now be potentially used for (64)Cu-based whole-body PET to detect T cell accumulation region with high-sensitivity, followed by SPION-based MRI of these regions for high-resolution anatomically correlated images of T cells. CD19-specific-CAR(+)SPION(pos) T cells effectively target in vitro CD19(+) lymphoma.
authors.affiliation: Department of Obstetrics & Gynecology, Baylor College of Medicine, Houston, TX 77030.
authors.forename: Parijat
authors.initials: P
authors.lastname: Bhatnagar
authors.affiliation: Department of Experimental Diagnostic Imaging, Division of Diagnostic Imaging, The University of Texas MD Anderson Cancer Center, Houston, TX 77030.
authors.forename: Mian
authors.initials: M
authors.lastname: Alauddin
authors.affiliation: Department of Imaging Physics, Division of Diagnostic Imaging, The University of Texas MD Anderson Cancer Center, Houston, TX 77030.
authors.forename: James A
authors.initials: JA
authors.lastname: Bankson
authors.affiliation: Department of Nanomedicine, The Methodist Hospital Research Institute, Houston, TX 77030.
authors.forename: Dickson
authors.initials: D
authors.lastname: Kirui
authors.affiliation: Department of Electrical and Computer Engineering, Rice University, Houston, TX 77005.
authors.forename: Payam
authors.initials: P
authors.lastname: Seifi
authors.affiliation: Division of Pediatrics, The University of Texas MD Anderson Cancer Center, Houston, TX 77030.
authors.forename: Helen
authors.initials: H
authors.lastname: Huls
authors.affiliation: 1] Division of Pediatrics, The University of Texas MD Anderson Cancer Center, Houston, TX 77030 [2] Department of Immunology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030.
authors.forename: Dean A
authors.initials: DA
authors.lastname: Lee
authors.affiliation: Department of Electrical and Computer Engineering, Rice University, Houston, TX 77005.
authors.forename: Aydin
authors.initials: A
authors.lastname: Babakhani
authors.affiliation: Department of Nanomedicine, The Methodist Hospital Research Institute, Houston, TX 77030.
authors.forename: Mauro
authors.initials: M
authors.lastname: Ferrari
authors.affiliation: 1] Department of Translational Imaging, The Methodist Hospital Research Institute, Houston, TX 77030 [2].
authors.forename: King C
authors.initials: KC
authors.lastname: Li
authors.affiliation: 1] Division of Pediatrics, The University of Texas MD Anderson Cancer Center, Houston, TX 77030 [2] Department of Immunology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030.
authors.forename: Laurence J N
authors.initials: LJ
authors.lastname: Cooper
mesh_terms: D019264:Adoptive Transfer; D018941:Antigens, CD19 / Q000378:metabolism; D002470:Cell Survival; D058948:Cell Tracking; D003287:Contrast Media; D018274:Electroporation; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods; D057054:Molecular Imaging / Q000379:methods*; D049268:Positron-Emission Tomography / Q000379:methods; D011485:Protein Binding; D019275:Radiopharmaceuticals; D011948:Receptors, Antigen, T-Cell / Q000235:genetics* / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics* / Q000378:metabolism*; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism*
chemical_list: D018941:Antigens, CD19; D003287:Contrast Media; D019275:Radiopharmaceuticals; D011948:Receptors, Antigen, T-Cell; D011993:Recombinant Fusion Proteins