title: Adaptation of Ti(III)-NTA colorimetric assay for use in detecting microbial demethylation of lignin and lignin derived compounds in aerobic conditions.
abstract: An anaerobic colorimetric assay for quantifying microbial demethylation activity was adapted for aerobic use in studying lignin and lignin-derived compounds. Standard curves of 0-500μM pyrocatechol with and without 0.3% lignin demonstrated the use in either case. This method detects demethylation products up to 500μM without using additional dilutions.
authors.affiliation: Biorefining Research Institute, Lakehead University, Thunder Bay, Ontario P7B 5E1, Canada; Department of Biology, Lakehead University, Thunder Bay, Ontario P7B 5E1, Canada. Electronic address: agibson@lakeheadu.ca.
authors.forename: A
authors.initials: A
authors.lastname: Gibson
authors.affiliation: Biorefining Research Institute, Lakehead University, Thunder Bay, Ontario P7B 5E1, Canada.
authors.forename: R F H
authors.initials: RF
authors.lastname: Dekker
authors.affiliation: Biorefining Research Institute, Lakehead University, Thunder Bay, Ontario P7B 5E1, Canada; Department of Biology, Lakehead University, Thunder Bay, Ontario P7B 5E1, Canada.
authors.forename: L
authors.initials: L
authors.lastname: Malek
mesh_terms: D020095:Absidia / Q000378:metabolism; D000693:Anaerobiosis; D018533:Biomass; D003124:Colorimetry / Q000379:methods*; D055453:Coriolaceae / Q000378:metabolism; D007220:Industrial Waste; D008031:Lignin / Q000032:analysis* / Q000737:chemistry* / Q000378:metabolism; D008745:Methylation; D009571:Nitrilotriacetic Acid / Q000737:chemistry*; D012037:Refuse Disposal; D014025:Titanium / Q000737:chemistry*
chemical_list: D007220:Industrial Waste; D008031:Lignin; D014025:Titanium; D009571:Nitrilotriacetic Acid
keywords: Demethylation; Lignin-derived compound; Ti(III)-NTA