title: Characterization of exopolymeric substances (EPS) produced by Aeromonas hydrophila under reducing conditions.
abstract: The aim of this work was to investigate the production of extracellular polymeric substances (EPS) by Aeromonas hydrophila grown under anaerobic conditions. EPS composition was studied for planktonic cells, cells attached to carbon fibre supports using a soluble ferric iron source and cells grown with a solid ferric iron mineral (gossan). Conventional spectrophotometric methods, Fourier transform infrared (FTIR) and confocal laser scanning microscopy (CLSM) were used to determine the main components in the biofilm extracted from the cultures. The key EPS components were proteins, indicating their importance for electron transfer reactions. Carbohydrates were observed mostly on the mineral and contained terminal mannosyl and/or terminal glucose, fucose and N-acetylgalactosamine residues.
authors.affiliation: a Department of Material Science and Metallurgical Engineering , Complutense University of Madrid , Madrid , Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: Castro
authors.forename: Ruiyong
authors.initials: R
authors.lastname: Zhang
authors.forename: Jesús A
authors.initials: JA
authors.lastname: Muñoz
authors.forename: Felisa
authors.initials: F
authors.lastname: González
authors.forename: M Luisa
authors.initials: ML
authors.lastname: Blázquez
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Sand
authors.forename: Antonio
authors.initials: A
authors.lastname: Ballester
mesh_terms: D016980:Aeromonas hydrophila / Q000378:metabolism*; D018441:Biofilms; D018613:Microscopy, Confocal; D011108:Polymers / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D011108:Polymers