title: Comparative proteomics of Euphorbia kansui Liou milky sap at two different developmental stages.
abstract: Euphorbia kansui Liou is a unique traditional Chinese medicinal herb. Its milky sap proteins play important roles in laticifer development, synthesis and transport of its biologically active substances. A proteomic approach was applied to analyze the E. kansui latex proteins related to laticifer development and secondary metabolite synthesis by using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and two-dimensional polyacrylamide gel electrophoresis. A total of 125 milky sap proteins associated with development of laticifers, disease and defense, and general metabolism were identified, and 19 differentially expressed proteins at two different developmental stages of laticifers were successfully detected. Peroxidase, cytochrome P450 mono-oxygenase superfamily, lipoxygenase, and multidrug resistance protein ABC transporter family may be involved in laticifer development, secondary metabolite synthesis and transport, and plant physiology.
authors.affiliation: Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education, Xi'an 710069, China.
authors.forename: Xueyan
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education, Xi'an 710069, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Si
authors.affiliation: Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education, Xi'an 710069, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Miao
authors.affiliation: Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education, Xi'an 710069, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Peng
authors.affiliation: School of Biological Science and Technology, Central South University, Changsha 410013, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.affiliation: Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education, Xi'an 710069, China. Electronic address: caix@nwu.edu.cn.
authors.forename: Xia
authors.initials: X
authors.lastname: Cai
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel; D028481:Euphorbia / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D010940:Plant Proteins
keywords: 2-DE; Euphorbia kansui Liou; MALDI-TOF MS; Milky sap; Protein; SDS-PAGE