title: Oral L-glutamine administration attenuated cutaneous wound healing in Wistar rats.
abstract: The objective of this study was to evaluate the wound healing potential of L-glutamine in laboratory rats using excision and incision wound models. Excision wounds of size 500 mm(2) and depth 2 mm were made on the dorsal portion of male Wistar rats (230-250 g) and were used for the study of oral L-glutamine (1 g/kg) treatment on the rate of contraction of wound and epithelisation. Histological evaluation of wound tissue was also performed. Six-centimetre-long two linear-paravertebral incisions in male Wistar rats (230-250 g) were used to study the effect of L-glutamine (1 g/kg, p.o.) treatment on tensile strength, total protein and hydroxyproline content in the incision model. Oral administration of L-glutamine (1 g/kg) significantly decreased wound area, epithelisation period and wound index, whereas the rate of wound contraction significantly increased (P < 0·001) when compared with vehicle control rats in the excision wound model. Tensile strength, hydroxyproline content and protein level were significantly increased (P < 0·001) in L-glutamine (1 g/kg, p.o.)-treated rats when compared with vehicle control rats in the incision wound model. Histological evaluation of wound tissue from L-glutamine (1 g/kg, p.o.)-treated rats showed complete epithelialisation with new blood vessel formation and high fibrous tissues in the excision wound model. In conclusion, oral administration of l-glutamine (1 g/kg) promotes wound healing by acting on various stages of wound healing such as collagen synthesis, wound contraction and epithelialisation.
authors.affiliation: Department of Surgery, Bharati Vidyapeeth Deemed University Medical College, Pune, India.
authors.forename: Saurabh
authors.initials: S
authors.lastname: Goswami
authors.affiliation: Department of Pharmacology, Poona College of Pharmacy, Bharati Vidyapeeth Deemed University, Pune, India.
authors.forename: Amit
authors.initials: A
authors.lastname: Kandhare
authors.affiliation: Department of Pharmacology, Poona College of Pharmacy, Bharati Vidyapeeth Deemed University, Pune, India.|Center for Innovation in Nutrition Health Disease, Interactive Research School for Health Affairs, Medical College Campus, Bharati Vidyapeeth Deemed University, Pune, India.
authors.forename: Anand A
authors.initials: AA
authors.lastname: Zanwar
authors.affiliation: Center for Innovation in Nutrition Health Disease, Interactive Research School for Health Affairs, Medical College Campus, Bharati Vidyapeeth Deemed University, Pune, India.
authors.forename: Mahabaleshwar V
authors.initials: MV
authors.lastname: Hegde
authors.affiliation: Department of Pharmacology, Poona College of Pharmacy, Bharati Vidyapeeth Deemed University, Pune, India.
authors.forename: Subhash L
authors.initials: SL
authors.lastname: Bodhankar
authors.affiliation: Department of Surgery, Bharati Vidyapeeth Deemed University Medical College, Pune, India.
authors.forename: Sudhir
authors.initials: S
authors.lastname: Shinde
authors.affiliation: Department of Surgery, Bharati Vidyapeeth Deemed University Medical College, Pune, India.
authors.forename: Shahaji
authors.initials: S
authors.lastname: Deshmukh
authors.affiliation: Department of Surgery, Bharati Vidyapeeth Deemed University Medical College, Pune, India.
authors.forename: Ravindran
authors.initials: R
authors.lastname: Kharat
mesh_terms: D000284:Administration, Oral; D000818:Animals; D005973:Glutamine / Q000494:pharmacology*; D006909:Hydroxyproline / Q000378:metabolism; D008297:Male; D018919:Neovascularization, Physiologic; D011506:Proteins / Q000378:metabolism; D017208:Rats, Wistar; D062326:Re-Epithelialization / Q000187:drug effects; D012867:Skin / Q000293:injuries* / Q000378:metabolism / Q000473:pathology; D013718:Tensile Strength; D014945:Wound Healing / Q000187:drug effects*; D014947:Wounds and Injuries / Q000378:metabolism / Q000473:pathology / Q000628:therapy*
chemical_list: D011506:Proteins; D005973:Glutamine; D006909:Hydroxyproline
keywords: Excision wounds; Hydroxyproline; Incision wounds; Tensile strength; l-Glutamine