title: Synthesis, characterization, cytotoxicity and DNA binding studies of a nNovel anionic organopalladium(II) complex.
abstract: A new anionic 8-hydroxyquinolinatopalladate(II) complex with malonate has been synthesized and characterized by elemental analysis, conductivity, FT-IR, UV-Vis and 1H NMR techniques to enhance the development of potential anticancer agents. Cytotoxicity was determined against the human leukemia cells, molt, by MTT assay. The novel antitumor Pd(II) complex was evaluated for its binding to calf thymus DNA (ctDNA) in physiological buffer (pH 7.0) by using absorption spectroscopy, fluorescence titration spectra, ethidium bromide displacement and gel chromatography studies. The results obtained from these analyses indicated that the water-soluble complex can bind to DNA cooperatively through a static quenching procedure at low concentrations. Thermodynamic parameters obtained from fluorescence experiments at different temperatures revealed the hydrogen binding and van der Waals force in the binding process which was supported by Scatchard's plots.
authors.forename: Maryam
authors.initials: M
authors.lastname: Saeidifar
authors.forename: Hassan
authors.initials: H
authors.lastname: Mansouri-Torshizi
authors.forename: Y
authors.initials: Y
authors.lastname: Palizdar
authors.forename: M
authors.initials: M
authors.lastname: Eslami-Moghaddam
authors.forename: Adeleh
authors.initials: A
authors.lastname: Divsalar
authors.forename: Ali Akbar
authors.initials: AA
authors.lastname: Saboury
mesh_terms: D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D045744:Cell Line, Tumor; D060326:Chemistry Techniques, Synthetic; D004247:DNA / Q000378:metabolism*; D006801:Humans; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D010165:Palladium / Q000737:chemistry*; D013816:Thermodynamics
chemical_list: D000970:Antineoplastic Agents; D009942:Organometallic Compounds; D010165:Palladium; D004247:DNA