title: Tomographic diffractive microscopy of living cells based on a common-path configuration.
abstract: We demonstrate a common-path tomographic diffractive microscopy technique for three-dimensional (3D) refractive-index (RI) imaging of unstained living cells. A diffraction grating is utilized to generate a reference beam that traverses a blank region of the sample in a common-path off-axis interferometry setup. Single-shot phase images captured at multiple illumination angles are used for 3D RI reconstruction based on optical diffraction tomography. The common-path configuration shows lower temporal phase fluctuations and better RI resolution than a Mach-Zehnder configuration. 3D subcellular RI distributions of live HeLa cells are quantified.
authors.forename: Wei-Chen
authors.initials: WC
authors.lastname: Hsu
authors.forename: Jing-Wei
authors.initials: JW
authors.lastname: Su
authors.forename: Te-Yu
authors.initials: TY
authors.lastname: Tseng
authors.forename: Kung-Bin
authors.initials: KB
authors.lastname: Sung
mesh_terms: D002470:Cell Survival; D006367:HeLa Cells; D006801:Humans; D021621:Imaging, Three-Dimensional; D008853:Microscopy / Q000379:methods*; D011137:Polystyrenes / Q000737:chemistry; D012031:Refractometry; D014054:Tomography / Q000379:methods*
chemical_list: D011137:Polystyrenes