title: Biochemical and radical-scavenging properties of sea cucumber (Stichopus vastus) collagen hydrolysates.
abstract: The molecular mass distribution, amino acid composition and radical-scavenging activity of collagen hydrolysates prepared from collagen isolated from the sea cucumber Stichopus vastus were investigated. β and α1 chains of the collagen were successfully hydrolysed by trypsin. The molecular mass distribution of the hydrolysates ranged from 5 to 25 kDa, and they were rich in glycine, alanine, glutamate, proline and hydroxyproline residues. The hydrolysates exhibited excellent radical-scavenging activity. These results indicate that collagen hydrolysates from S. vastus can be used as a functional ingredient in food and nutraceutical products.
authors.affiliation: a Food Biopolymer Research Group, Food Technology Division, School of Industrial Technology, Universiti Sains Malaysia , 11800 Penang , Malaysia.
authors.forename: Md Zainul
authors.initials: MZ
authors.lastname: Abedin
authors.forename: Alias A
authors.initials: AA
authors.lastname: Karim
authors.forename: Aishah A
authors.initials: AA
authors.lastname: Latiff
authors.forename: Chee-Yuen
authors.initials: CY
authors.lastname: Gan
authors.forename: Farid Che
authors.initials: FC
authors.lastname: Ghazali
authors.forename: Zoha
authors.initials: Z
authors.lastname: Barzideh
authors.forename: Sahena
authors.initials: S
authors.lastname: Ferdosh
authors.forename: Md Jahurul Haque
authors.initials: MJ
authors.lastname: Akanda
authors.forename: Wahidu
authors.initials: W
authors.lastname: Zzaman
authors.forename: Md Rezaul
authors.initials: MR
authors.lastname: Karim
authors.forename: Md Zaidul Islam
authors.initials: MZ
authors.lastname: Sarker
mesh_terms: D000409:Alanine / Q000032:analysis; D000596:Amino Acids / Q000032:analysis*; D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D003094:Collagen / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D018698:Glutamic Acid / Q000032:analysis; D005998:Glycine / Q000032:analysis; D006868:Hydrolysis; D006909:Hydroxyproline / Q000032:analysis; D015394:Molecular Structure; D008970:Molecular Weight; D011392:Proline / Q000032:analysis; D011492:Protein Hydrolysates / Q000737:chemistry*; D047528:Stichopus / Q000737:chemistry*
chemical_list: D000596:Amino Acids; D000975:Antioxidants; D016166:Free Radical Scavengers; D011492:Protein Hydrolysates; D018698:Glutamic Acid; D003094:Collagen; D011392:Proline; D000409:Alanine; D006909:Hydroxyproline; D005998:Glycine
keywords: biochemical and radical scavenging properties; collagen hydrolysates; integument collagen; sea cucumber; trypsin hydrolysis