title: The low chamber pancreatic cancer cells had stem-like characteristics in modified transwell system: is it a novel method to identify and enrich cancer stem-like cells?
abstract: Cancer stem cells (CSCs) or cancer-initiating cells (CICs) play an important role in tumor initiation, progression, metastasis, chemoresistance, and recurrence. It is important to construct an effective method to identify and isolate CSCs for biotherapy of cancer. During the past years, many researchers had paid more attention to it; however, this method was still on seeking. Therefore, compared to the former methods that were used to isolate the cancer stem cell, in the present study, we tried to use modified transwell system to isolate and enrich CSCs from human pancreatic cancer cell lines (Panc-1). Our results clearly showed that the lower chamber cells in modified transwell system were easily forming spheres; furthermore, these spheres expressed high levels of stem cell markers (CD133/CD44/CD24/Oct-4/ESA) and exhibited chemoresistance, underwent epithelial-to-mesenchymal transition (EMT), and possessed the properties of self-renewal in vitro and tumorigenicity in vivo. Therefore, we speculated that modified transwell assay system, as a rapid and effective method, can be used to isolate and enrich CSCs.
authors.affiliation: The Affiliated Hospital of Jiangsu University, Zhenjiang 212001, China.
authors.forename: Dongqing
authors.initials: D
authors.lastname: Wang
authors.affiliation: The Affiliated Hospital of Jiangsu University, Zhenjiang 212001, China.
authors.forename: Haitao
authors.initials: H
authors.lastname: Zhu
authors.affiliation: The Affiliated Hospital of Jiangsu University, Zhenjiang 212001, China.
authors.forename: Yanfang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: The Affiliated Hospital of Jiangsu University, Zhenjiang 212001, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Liu
authors.affiliation: The Affiliated Hospital of Jiangsu University, Zhenjiang 212001, China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Xie
authors.affiliation: The Affiliated Hospital of Jiangsu University, Zhenjiang 212001, China.
authors.forename: Yuepeng
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: The Affiliated Hospital of Jiangsu University, Zhenjiang 212001, China.
authors.forename: Lirong
authors.initials: L
authors.lastname: Zhang
authors.affiliation: The Affiliated Hospital of Jiangsu University, Zhenjiang 212001, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Department of Histology and Embryology, Center of Clinical Medicine and Laboratory, Jiangsu University, 301 Xuefu Road, Zhenjiang, Jiangsu 212013, China.
authors.forename: Zhijian
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Immunology, Center of Clinical Medicine and Laboratory, Jiangsu University, 301 Xuefu Road, Zhenjiang, Jiangsu 212013, China.
authors.forename: Zhaoliang
authors.initials: Z
authors.lastname: Su
mesh_terms: D000071916:AC133 Antigen; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000378:metabolism; D063646:Carcinogenesis / Q000473:pathology; D002452:Cell Count; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003841:Deoxycytidine / Q000031:analogs & derivatives / Q000494:pharmacology; D019008:Drug Resistance, Neoplasm / Q000187:drug effects; D058750:Epithelial-Mesenchymal Transition / Q000187:drug effects; D006023:Glycoproteins / Q000378:metabolism; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D014411:Neoplastic Stem Cells / Q000187:drug effects / Q000473:pathology*; D010190:Pancreatic Neoplasms / Q000473:pathology*; D010455:Peptides / Q000378:metabolism; D018874:Spheroids, Cellular / Q000187:drug effects / Q000473:pathology; D015854:Up-Regulation / Q000187:drug effects; D000093542:Gemcitabine
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D014408:Biomarkers, Tumor; D006023:Glycoproteins; D018960:Hyaluronan Receptors; C000606023:PROM1 protein, human; D010455:Peptides; C000606024:Prom1 protein, mouse; D003841:Deoxycytidine; D000093542:Gemcitabine