title: MicroRNA-424 is down-regulated in hepatocellular carcinoma and suppresses cell migration and invasion through c-Myb.
abstract: Hepatocellular carcinoma (HCC) is one of the leading causes of cancer-related mortality worldwide. MicroRNAs (miRNAs) are important regulators of multiple cellular processes, and the aberrant miRNAs expressions have been observed in different types of cancer including HCC. Their pathysiologic role and their relevance to tumorigenesis are still largely unknown. In this study, we demonstrated the down-regulation of miR-424 in HCC cell lines and tissues by quantitative RT-PCR analyses. Overexpression of miR-424 reduced the HCC cell prolifetation, migration, and invasion. Conversely, inhibiton of miR-424 expression significantly accelerated the cell proliferation, migration, and invasion. In addition, we further identified c-Myb as a functional downstream target of miR-424 by directly targeting the 3'UTR of c-Myb. Furthermore, overexpression of c-Myb impaired miR-424-induced inhibition of proliferation and invasion in HCC cells. Our results demonstrated that miR-424 was involved in tumorigenesis of HCC at least in part by suppression of c-Myb.
authors.affiliation: Department of Infectious Disease, The Fourth Hospital of Harbin Medical University, Harbin, Heilongjiang, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Yu
authors.affiliation: Department of Infectious Disease, Binzhou Medical University Hospital, Binzhou, Shandong, China.
authors.forename: Guo-feng
authors.initials: GF
authors.lastname: Ding
authors.affiliation: The Graduate School of Harbin Medical University, Harbin, Heilongjiang, China.
authors.forename: Changzhi
authors.initials: C
authors.lastname: He
authors.affiliation: Department of Ophthalmology, The Fourth Hospital of Harbin Medical University, Harbin, Heilongjiang, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Sun
authors.affiliation: Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun, Jilin, China; Department of Central Laboratory, the Second Part of First Hospital, Jilin University, Changchun, Jilin, China.
authors.forename: YanFang
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Department of Infectious Disease, The Fourth Hospital of Harbin Medical University, Harbin, Heilongjiang, China.
authors.forename: Liying
authors.initials: L
authors.lastname: Zhu
mesh_terms: D020413:3' Untranslated Regions; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D020570:Genes, myb; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D020413:3' Untranslated Regions; C554214:MIRN424 microrna, human; D035683:MicroRNAs; D012333:RNA, Messenger