title: Detection of peptidic erythropoiesis-stimulating agents in sport.
abstract: Detection methods for erythropoiesis-stimulating agents in sport can be classified into direct and indirect approaches. Direct methods comprise electrophoretic techniques (isoelectric focusing (IEF-), sodium-dodecylsulfate (SDS-), sarcosyl (SAR-) polyacrylamide gel-electrophoreses (-PAGE)), ELISAs and mass spectrometric methods. The haematological module of the Athlete Biological Passport is currently the only applied indirect approach. Newer developments include a mass spectrometric test for peginesatide, sequential exoglycosidase digestion of ertythropoietin (EPO) combined with electrophoresis (SDS/SAR-PAGE), a dipstick method (MAIIA), and a study on the differences in sialic acid O-acetylation of tryptic EPO O-glycopeptides. The focus of this article is on direct detection methods.
authors.affiliation: Doping Control Laboratory, AIT Seibersdorf Labor GmbH, , Seibersdorf, Austria.
authors.forename: Christian
authors.initials: C
authors.lastname: Reichel
mesh_terms: D004300:Doping in Sports / Q000517:prevention & control*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006397:Hematinics / Q000032:analysis*; D006801:Humans; D007525:Isoelectric Focusing; D013058:Mass Spectrometry; D058573:Performance-Enhancing Substances / Q000032:analysis*; D015813:Substance Abuse Detection / Q000379:methods*
chemical_list: D006397:Hematinics; D058573:Performance-Enhancing Substances
keywords: Anaemia; Biochemistry