title: Analysis of gene expression changes, caused by exposure to nitrite, in metabolic and antioxidant enzymes in the red claw crayfish, Cherax quadricarinatus.
abstract: We evaluated the effect of acute exposure to nitrite on expression of antioxidant and metabolic enzyme genes in gill tissue of advanced juvenile Cherax quadricarinatus. A 48h nitrite exposure was conducted, using four test concentrations (NO2-N=0.5, 1, 1.5 and 2mg L(-1)) plus a control group. The relative mRNA expression of mitochondrial manganese superoxide dismutase (mMnSOD), cytosolic MnSOD (cMnSOD), extracellular copper/zinc SOD (exCu/ZnSOD), catalase (CAT), glutathione S-transferase (GST), arginine kinase (AK), glutamate dehydrogenase (GDH), mitochondrial malate dehydrogenase (mMDH), Na(+)/K(+)-ATPase α-subunit and phosphoenolpyruvate carboxykinase (PEPCK) in gill tissue was measured. Significantly increased mRNA expression was observed for all the antioxidant enzymes after 12 and 24h. After 48h, they all decreased at high nitrite concentrations. The gene expression levels of AK, GDH, mMDH and Na(+)/K(+)-ATPase α-subunit showed similar trends as the antioxidant enzymes. Significant depression of gene expression levels of PEPCK occurred throughout the experimental time at high nitrite concentrations. The results indicated that nitrite could induce oxidative and metabolic stress in C. quadricarinatus, in a time dependent manner, which suggests they could be helpful in predicting sublethal nitrite toxicity and useful in environmental monitoring studies.
authors.affiliation: Jiangsu Province Key Laboratory for Aquatic Live Food, Jiangsu Province Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, 1 Wenyuan Road, Nanjing 210023, PR China.
authors.forename: Qichen
authors.initials: Q
authors.lastname: Jiang
authors.affiliation: Jiangsu Province Key Laboratory for Aquatic Live Food, Jiangsu Province Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, 1 Wenyuan Road, Nanjing 210023, PR China.
authors.forename: Wenyi
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Jiangsu Province Key Laboratory for Aquatic Live Food, Jiangsu Province Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, 1 Wenyuan Road, Nanjing 210023, PR China.
authors.forename: Hongyue
authors.initials: H
authors.lastname: Tan
authors.affiliation: Jiangsu Province Key Laboratory for Aquatic Live Food, Jiangsu Province Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, 1 Wenyuan Road, Nanjing 210023, PR China.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Pan
authors.affiliation: Jiangsu Province Key Laboratory for Aquatic Live Food, Jiangsu Province Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, 1 Wenyuan Road, Nanjing 210023, PR China.
authors.forename: Yuanhao
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Jiangsu Province Key Laboratory for Aquatic Live Food, Jiangsu Province Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, 1 Wenyuan Road, Nanjing 210023, PR China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Ren
authors.affiliation: Jiangsu Province Key Laboratory for Aquatic Live Food, Jiangsu Province Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, 1 Wenyuan Road, Nanjing 210023, PR China. Electronic address: jyangnjnu@gmail.com.
authors.forename: Jiaxin
authors.initials: J
authors.lastname: Yang
mesh_terms: D000818:Animals; D003400:Astacoidea / Q000187:drug effects* / Q000235:genetics; D004798:Enzymes / Q000235:genetics* / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D005880:Gills / Q000187:drug effects / Q000201:enzymology; D008297:Male; D009573:Nitrites / Q000633:toxicity*; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D004798:Enzymes; D009573:Nitrites; D014874:Water Pollutants, Chemical; D010088:Oxidoreductases
keywords: Antioxidant enzyme; Cherax quadricarinatus; Gene expression; Metabolic enzyme; Nitrite