title: Antiviral effect of flavonol glycosides isolated from the leaf of Zanthoxylum piperitum on influenza virus.
abstract: The ethanol extract of Zanthoxylum piperitum (L.) DC. showed in vitro antiviral activity against influenza A virus. Three flavonol glycosides were isolated from the EtOAc fraction of Z. piperitum leaf by means of activity-guided chromatographic separation. Structures of isolated compounds were identified as quercetin 3-O-β-D-galactopyranoside (1), quercetin 3-O-α-L-rhamnopyranoside (2), kaempferol 3-O-α-L-rhamnopyranoside (3) by comparing their spectral data with literature values. The anti-influenza viral activity of isolates was evaluated using a plaque reduction assay against influenza A/NWS/33 (H1N1) virus. The compounds also were subjected to neuraminidase inhibition assay in influenza A/NWS/33 virus. Compounds 1-3 exhibited antiviral activity against an influenza A virus in vitro, and inhibited the neuraminidase activity at relatively high concentrations.
authors.affiliation: School of Pharmacy, Sungkyunkwan University, Suwon, 440-746, Republic of Korea.
authors.forename: Song-Yi
authors.initials: SY
authors.lastname: Ha
authors.forename: Hana
authors.initials: H
authors.lastname: Youn
authors.forename: Chang-Seon
authors.initials: CS
authors.lastname: Song
authors.forename: Se Chan
authors.initials: SC
authors.lastname: Kang
authors.forename: Jong Jin
authors.initials: JJ
authors.lastname: Bae
authors.forename: Hee Tae
authors.initials: HT
authors.lastname: Kim
authors.forename: Kwang Min
authors.initials: KM
authors.lastname: Lee
authors.forename: Tae Hoon
authors.initials: TH
authors.lastname: Eom
authors.forename: In Su
authors.initials: IS
authors.lastname: Kim
authors.forename: Jong Hwan
authors.initials: JH
authors.lastname: Kwak
mesh_terms: D000998:Antiviral Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002845:Chromatography; D044948:Flavonols / Q000302:isolation & purification / Q000494:pharmacology*; D006027:Glycosides / Q000302:isolation & purification / Q000494:pharmacology*; D053118:Influenza A Virus, H1N1 Subtype / Q000187:drug effects*; D009439:Neuraminidase / Q000037:antagonists & inhibitors; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D010948:Viral Plaque Assay; D014764:Viral Proteins / Q000037:antagonists & inhibitors; D032106:Zanthoxylum / Q000737:chemistry*
chemical_list: D000998:Antiviral Agents; D044948:Flavonols; D006027:Glycosides; D010936:Plant Extracts; D014764:Viral Proteins; C487630:NA protein, influenza A virus; D009439:Neuraminidase