title: In vivo effects of 17β-estradiol on cardiac Na(+)/K(+)-ATPase expression and activity in rat heart.
abstract: In this study the in vivo effects of estradiol in regulating Na(+)/K(+)-ATPase function in rat heart was studied. Adult male Wistar rats were treated with estradiol (40μg/kg, i.p.) and after 24h the animals were sacrificed and the heart excised. Following estradiol administration, cardiac Na(+)/K(+)-ATPase activity, expression of the α1 subunit, and phosphorylation of the α1 subunit were significantly increased. These animals also had significantly decreased levels of digoxin-like immunoreactive factor(s). Na(+) levels were also significantly reduced but to a level that was still within the normal physiological range, highlighting the ability of the Na(+)/K(+)-ATPase to balance the ionic composition following treatment with estradiol. Estradiol treated rats also showed increased phosphorylation of protein kinase B (Akt), and extracellular-signal-regulated kinase 1/2 (ERK1/2). We therefore suggest a role for Akt and/or ERK1/2 in estradiol-mediated regulation of cardiac Na(+)/K(+)-ATPase expression and activity in rat heart.
authors.affiliation: Institute of Nuclear Sciences Vinca, University of Belgrade, Laboratory of Radiobiology and Molecular Genetics, Mike Petrovica Alasa 12-14, 11000 Belgrade, Serbia. Electronic address: obradovicmilan@hotmail.com.
authors.forename: Milan
authors.initials: M
authors.lastname: Obradovic
authors.affiliation: School of Medicine, University of St Andrews, North Haugh, St Andrews KY16 9TF, United Kingdom. Electronic address: ajs21@st-andrews.ac.uk.
authors.forename: Alan J
authors.initials: AJ
authors.lastname: Stewart
authors.affiliation: School of Medicine, University of St Andrews, North Haugh, St Andrews KY16 9TF, United Kingdom. Electronic address: sjp24@st-andrews.ac.uk.
authors.forename: Samantha J
authors.initials: SJ
authors.lastname: Pitt
authors.affiliation: Institute of Histology and Embryology "Aleksandar Đ. Kostić", Faculty of Medicine, University of Belgrade, Visegradska 26, 11000 Belgrade, Serbia. Electronic address: sborovic2001@yahoo.com.
authors.forename: Milica
authors.initials: M
authors.lastname: Labudovic-Borovic
authors.affiliation: Institute of Nuclear Sciences Vinca, University of Belgrade, Laboratory of Radiobiology and Molecular Genetics, Mike Petrovica Alasa 12-14, 11000 Belgrade, Serbia. Electronic address: emma_crash@yahoo.com.
authors.forename: Emina
authors.initials: E
authors.lastname: Sudar
authors.affiliation: Institute of Nuclear Sciences Vinca, University of Belgrade, Laboratory of Physical Chemistry, Mike Petrovica Alasa 12-14, 11000 Belgrade, Serbia. Electronic address: onagrus@yahoo.com.
authors.forename: Voin
authors.initials: V
authors.lastname: Petrovic
authors.affiliation: Institute of Nuclear Sciences Vinca, University of Belgrade, Laboratory of Radiobiology and Molecular Genetics, Mike Petrovica Alasa 12-14, 11000 Belgrade, Serbia. Electronic address: sonjazafirovic@hotmail.com.
authors.forename: Sonja
authors.initials: S
authors.lastname: Zafirovic
authors.affiliation: Dedinje Cardiovascular Institute, Belgrade University, School of Medicine, Heroja Milana Tepica 1, 11000 Belgrade, Serbia. Electronic address: vmaravic@ikvbd.com.
authors.forename: Vera
authors.initials: V
authors.lastname: Maravic-Stojkovic
authors.affiliation: Institute of Nuclear Sciences Vinca, University of Belgrade, Laboratory of Physical Chemistry, Mike Petrovica Alasa 12-14, 11000 Belgrade, Serbia. Electronic address: evasic@vinca.rs.
authors.forename: Vesna
authors.initials: V
authors.lastname: Vasic
authors.affiliation: Institute of Nuclear Sciences Vinca, University of Belgrade, Laboratory of Radiobiology and Molecular Genetics, Mike Petrovica Alasa 12-14, 11000 Belgrade, Serbia. Electronic address: isenovic@yahoo.com.
authors.forename: Esma R
authors.initials: ER
authors.lastname: Isenovic
mesh_terms: D000818:Animals; D002298:Cardenolides / Q000097:blood; D002462:Cell Membrane / Q000201:enzymology; D002784:Cholesterol / Q000097:blood; D004958:Estradiol / Q000502:physiology*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D015870:Gene Expression; D008297:Male; D009206:Myocardium / Q000201:enzymology*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D016755:Proto-Oncogene Proteins c-jun / Q000378:metabolism; D016271:Proto-Oncogene Proteins c-myc / Q000378:metabolism; D017208:Rats, Wistar; D012503:Saponins / Q000097:blood; D015398:Signal Transduction; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics / Q000378:metabolism*
chemical_list: D002298:Cardenolides; D016755:Proto-Oncogene Proteins c-jun; D016271:Proto-Oncogene Proteins c-myc; D012503:Saponins; C034504:digoxin-like factors; D004958:Estradiol; D002784:Cholesterol; D051057:Proto-Oncogene Proteins c-akt; D048049:Extracellular Signal-Regulated MAP Kinases; D000254:Sodium-Potassium-Exchanging ATPase
keywords: 5-bromo-4-chloro-3-indoyl phosphate/nitro blue tetrazolium chloride; ALP; Akt; BCIP/NBT; CV; DLIF; ER; ERK1/2; Estradiol; Extracellular-signal-regulated kinase ½; FPIA; HRP; IR; IRS1; LV; Na(+)/K(+)-ATPase; PBS; PI3K; PVDF; Protein kinase B; RIA; Sodium potassium adenosine-triphosphatase; TBS; TC; alkaline phosphatase; cardiovascular; digoxin-like immunoreactive factor(s); estrogen receptor; extracellular-signal-regulated kinase 1/2; fluorescence polarization immunoassay; horseradish peroxidase; insulin receptor substrate 1; insulin resistance; left ventricle; phosphate-buffered saline; phosphatidylinositol-3 kinase; polyvinylidence difluoride; protein kinase B; radioimmunoassay; sodium/potassium-adenosine triphosphatase; total cholesterol; tris (hydroxymethyl) aminomethane buffer saline