title: The expression and activity of 5-LOX in the large intestine of horses harbouring encysted cyathostomin larvae.
abstract: Leukotrienes are products of the arachidonic acid metabolism and act as potent inflammatory mediators modulating the immune response and various physiological processes. This study evaluated the expression and activity of 5-lipoxygenase (5-LOX), the enzyme that catalyzes the first two steps in the biosynthesis of leukotrienes, in horses infected by larval cyathostomins. Tissue samples from dorsal and ventral colon, and from the cecum were collected from 16 horses slaughtered for human consumption. Samples were analyzed to estimate the burdens of encysted cyathostomin larvae and adult luminal stages, and then processed for the evaluation of biochemical parameters. No significant differences were found in the protein expression and enzymatic activity of 5-LOX between animals harbouring only adult parasites and negative horses. The protein expression and enzyme activity of 5-LOX were significantly higher in horses harbouring encysted larvae in comparison with horses free of encysted larvae. Although preliminary, these results indicate that 5-LOX is an important mediator in the course of horse cyathostominosis and further studies are warranted to unveil the possible role this enzyme plays in the pathogenesis of horse cyathostominosis, and its potential as a diagnostic marker.
authors.affiliation: Faculty of Veterinary Medicine, University of Teramo, Piazza A. Moro 45, 64100 Teramo, Italy.
authors.forename: Roberto
authors.initials: R
authors.lastname: Giacominelli-Stuffler
authors.affiliation: Department of Animal Medicine, Production and Health, University of Padova, Viale dell'Università 16, 35020 Legnaro, PD, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Frangipane di Regalbono
authors.affiliation: Faculty of Veterinary Medicine, University of Teramo, Piazza A. Moro 45, 64100 Teramo, Italy. Electronic address: dtraversa@unite.it.
authors.forename: Donato
authors.initials: D
authors.lastname: Traversa
authors.affiliation: Zoetis, VMRD, Mercuriuslaan 20, 1930 Zaventem, Belgium.
authors.forename: Thomas
authors.initials: T
authors.lastname: Geurden
authors.affiliation: Department of Animal Medicine, Production and Health, University of Padova, Viale dell'Università 16, 35020 Legnaro, PD, Italy.
authors.forename: Federica
authors.initials: F
authors.lastname: Marcer
authors.affiliation: Faculty of Veterinary Medicine, University of Teramo, Piazza A. Moro 45, 64100 Teramo, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Di Francesco
authors.affiliation: Faculty of Veterinary Medicine, University of Teramo, Piazza A. Moro 45, 64100 Teramo, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Angelini
authors.affiliation: Faculty of Veterinary Medicine, University of Teramo, Piazza A. Moro 45, 64100 Teramo, Italy.
authors.forename: Angela
authors.initials: A
authors.lastname: di Cesare
authors.affiliation: Department of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari, Strada Prov. le per Casamassima, Km 3, 70010 Valenzano, BA, Italy.
authors.forename: Maria Maddalena
authors.initials: MM
authors.lastname: Storelli
authors.affiliation: Center of Integrated Research, Campus Bio-Medico University of Rome, Via Alvaro del Portillo 21, 00128 Rome, Italy; European Center for Brain Research/Santa Lucia Foundation, Via del fosso di Fiorano, 00146 Rome, Italy.
authors.forename: Mauro
authors.initials: M
authors.lastname: Maccarrone
mesh_terms: D000818:Animals; D001094:Arachidonate 5-Lipoxygenase / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic / Q000276:immunology*; D006736:Horses; D007420:Intestine, Large / Q000201:enzymology / Q000276:immunology*; D008018:Life Cycle Stages / Q000276:immunology; D013319:Strongyle Infections, Equine / Q000201:enzymology / Q000276:immunology*; D059467:Transcriptome
chemical_list: D001094:Arachidonate 5-Lipoxygenase
keywords: 5-Lipoxygenase; Cyathostominosis; Diagnosis; Eicosanoids; Horse; Leukotrienes