title: Globally visualizing the microtubule-dependent transport behaviors of influenza virus in live cells.
abstract: Understanding the microtubule-dependent behaviors of viruses in live cells is very meaningful for revealing the mechanisms of virus infection and endocytosis. Herein, we used a quantum dots-based single-particle tracking technique to dynamically and globally visualize the microtubule-dependent transport behaviors of influenza virus in live cells. We found that the intersection configuration of microtubules can interfere with the transport behaviors of the virus in live cells, which lead to the changing and long-time pausing of the transport behavior of viruses. Our results revealed that most of the viruses moved along straight microtubules rapidly and unidirectionally from the cell periphery to the microtubule organizing center (MTOC) near the bottom of the cell, and the viruses were confined in the grid of microtubules near the top of the cell and at the MTOC near the bottom of the cell. These results provided deep insights into the influence of entire microtubule geometry on the virus infection.
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, State Key Laboratory of Virology, and Wuhan Institute of Biotechnology, Wuhan University , Wuhan, Hubei 430072, P.R. China.
authors.forename: Shu-Lin
authors.initials: SL
authors.lastname: Liu
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Zhang
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Wang
authors.forename: Zhi-Ling
authors.initials: ZL
authors.lastname: Zhang
authors.forename: Qiu-Mei
authors.initials: QM
authors.lastname: Wu
authors.forename: En-Ze
authors.initials: EZ
authors.lastname: Sun
authors.forename: Yun-Bo
authors.initials: YB
authors.lastname: Shi
authors.forename: Dai-Wen
authors.initials: DW
authors.lastname: Pang
mesh_terms: D000818:Animals; D002477:Cells / Q000648:ultrastructure* / Q000821:virology*; D004285:Dogs; D004705:Endocytosis; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007251:Influenza, Human / Q000821:virology; D061985:Madin Darby Canine Kidney Cells; D008856:Microscopy, Fluorescence; D008870:Microtubules / Q000648:ultrastructure* / Q000821:virology*; D009975:Orthomyxoviridae / Q000648:ultrastructure*; D045663:Quantum Dots; D014759:Viral Envelope Proteins / Q000737:chemistry
chemical_list: D014759:Viral Envelope Proteins