title: Determination of synthetic food dyes in commercial soft drinks by TLC and ion-pair HPLC.
abstract: Synthetic food colourings were analyzed on commercial carbonated orange and grape soft drinks produced in Ceará State, Brazil. Tartrazine (E102), Amaranth (E123), Sunset Yellow (E110) and Brilliant Blue (E133) were extracted from soft drinks using C18 SPE and identified by thin layer chromatography (TLC), this method was used to confirm the composition of food colouring in soft drinks stated on label. The concentration of food colouring in soft drink was determined by ion-pair high performance liquid chromatography with photodiode array detection. The results obtained with the samples confirm that the identification and quantification methods are recommended for quality control of the synthetic colours in soft drinks, as well as to determine whether the levels and lables complies with the recommendations of food dyes legislation.
authors.affiliation: Department of Public Health, State University of Ceará, Brazil.
authors.forename: Francisca Ivani
authors.initials: FI
authors.lastname: de Andrade
authors.affiliation: Department of Nutrition, State University of Ceará, Brazil. Electronic address: florinfg@terra.com.br.
authors.forename: Maria Izabel
authors.initials: MI
authors.lastname: Florindo Guedes
authors.affiliation: Technological Development Park, Federal University of Ceará, Brazil.
authors.forename: Ícaro Gusmão
authors.initials: ÍG
authors.lastname: Pinto Vieira
authors.affiliation: Technological Development Park, Federal University of Ceará, Brazil.
authors.forename: Francisca Noélia
authors.initials: FN
authors.lastname: Pereira Mendes
authors.affiliation: Department of Nutrition and Health, State University of Ceará, Brazil.
authors.forename: Paula Alves
authors.initials: PA
authors.lastname: Salmito Rodrigues
authors.affiliation: Department of Nutrition, State University of Ceará, Brazil.
authors.forename: Carla Soraya
authors.initials: CS
authors.lastname: Costa Maia
authors.affiliation: Department of Public Health, State University of Ceará, Brazil.
authors.forename: Maria Marlene
authors.initials: MM
authors.lastname: Marques Ávila
authors.affiliation: Department of Chemistry, State University of Ceará, Brazil.
authors.forename: Luzara
authors.initials: L
authors.lastname: de Matos Ribeiro
mesh_terms: D002253:Carbonated Beverages / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002855:Chromatography, Thin Layer / Q000379:methods*; D004396:Coloring Agents / Q000032:analysis*; D005505:Food Coloring Agents / Q000032:analysis*; D015394:Molecular Structure
chemical_list: D004396:Coloring Agents; D005505:Food Coloring Agents
keywords: HPLC; Synthetic food colouring; Thin layer chromatography