title: Niabella thaonhiensis sp. nov., isolated from the forest soil of Kyonggi University in Korea.
abstract: Strain NHI-24(T) was isolated from forest soil by a polycarbonate membrane transwell plate. It is a Gram-negative, rod-shaped, non-motile, non-spore-forming bacterium. Phylogenetic analysis based on 16S rRNA gene sequence data indicated that strain NHI-24(T) is closely related to members of the genus Niabella: N. drilacis E90(T) (97.7 %), N. tibetensis 15-4(T) (96.7 %), N. aurantiaca R2A15-11(T) (96.5 %), N. hirudinis E96(T) (95.8 %), N. soli JS13-8(T) (94.7 %), N. ginsengisoli NBRC106414(T) (94.4 %), and N. yanshanensis CCBAU 05354(T) (94.2 %). Growth temperatures range widely, from 15 to 37 °C, with 30 °C as the optimum. Salt tolerance ranges from 0 to 2 %. The strain grows at pH 6.5-11.0, with an optimal range of pH 7.0-9.5. Cells produce flexirubin-type pigments, and the predominant menaquinone is MK-7. The major fatty acids of strain NHI-24(T) are iso-C15:0 (36.72 %), iso-C15:1 G (20.8 %), and summed feature 3 (C16:1 ω7c/C16:1 ω6c; 15.2 %). DNA-DNA hybridization values between strain NHI-24(T) and members of the genus Niabella range from 37 to 53 %. Based on these results, it is proposed that strain NHI-24(T) represents a novel species of the genus Niabella with the name Niabella thaonhiensis (= KACC 17215(T) = KEMB 9005-018(T) = JCM 18864(T)).
authors.affiliation: Department of Life Science, Graduate School of Kyonggi University, Suwon, Gyeonggi-Do, 443-760, South Korea.
authors.forename: Van H T
authors.initials: VH
authors.lastname: Pham
authors.forename: Jaisoo
authors.initials: J
authors.lastname: Kim
mesh_terms: D041963:Bacteroidetes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D016000:Cluster Analysis; D003600:Cytosol / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D065928:Forests; D006863:Hydrogen-Ion Concentration; D007723:Korea; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D010860:Pigments, Biological; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013696:Temperature; D014495:Universities; D024482:Vitamin K 2 / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010860:Pigments, Biological; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2