title: Preparation of herpes simplex virus-infected primary neurons for transmission electron microscopy.
abstract: Transmission electron microscopy (TEM) provides the resolution necessary to identify both viruses and subcellular components of cells infected with many types of viruses, including herpes simplex virus. Recognized as a powerful tool in both diagnostic and research-based virology laboratories, TEM has made possible the identification of new viruses and has contributed to the elucidation of virus life cycle and virus-host cell interaction. Whilst there are many sample preparation techniques for TEM, conventional processing using chemical fixation and resin embedding remains a useful technique, available in virtually all EM laboratories, for studying virus/cell ultrastructure. In this chapter, we describe the preparation of herpes simplex virus-infected primary neurons, grown on plastic cover slips, to allow sectioning of neurons and axons in their growth plane. This technique allows TEM examination of cell bodies, axons, growth cones, and varicosities, providing powerful insights into virus-cell interaction.
authors.affiliation: Centre for Virus Research, Westmead Millennium Institute, The University of Sydney, Darcy Road, Westmead, NSW, 2145, Australia, monica.miranda@sydney.edu.au.
authors.forename: Monica
authors.initials: M
authors.lastname: Miranda-Saksena
authors.forename: Ross
authors.initials: R
authors.lastname: Boadle
authors.forename: Anthony L
authors.initials: AL
authors.lastname: Cunningham
mesh_terms: D001369:Axons / Q000473:pathology / Q000648:ultrastructure* / Q000821:virology; D006561:Herpes Simplex / Q000175:diagnosis / Q000473:pathology / Q000821:virology; D006801:Humans; D046529:Microscopy, Electron, Transmission; D008967:Molecular Biology / Q000379:methods*; D009474:Neurons / Q000473:pathology / Q000648:ultrastructure* / Q000821:virology; D061251:Primary Cell Culture; D018139:Simplexvirus / Q000235:genetics* / Q000378:metabolism