title: Single tube genotyping of TYMS 1494del6 polymorphism in the Chinese Han population by duplex scorpion primers.
abstract: BACKGROUND
  The development of pharmacogenomics has created an urgent need for robust molecular characterization. And it has become a challenge to develop suitable detecting methods for routine clinical use.
  
  
  OBJECTIVE
  The aim of the current study is to develop a simple and reliable TYMS 1494del6 polymorphism genotyping assay by duplex scorpion primers in the Chinese Han population.
  
  
  METHODS
  We evaluated the performance of the duplex scorpion primer assay in the genotyping of TYMS 1494del6 polymorphism and screened 54 DNA samples of the Chinese Han population. The results were further validated by pyrosequencing.
  
  
  RESULTS
  The duplex scorpion primer assay showed high specificity and accuracy for genotyping TYMS 1494del6 polymorphism. Complete concordance was observed between the duplex scorpion primer assay and pyrosequencing. The frequency of the TYMS +6 bp allele was 34% and the -6 bp allele was 66% in 54 Chinese Han population DNA samples.
  
  
  CONCLUSIONS
  The duplex scorpion primer assay provides a rapid, reliable and high-throughput method to genotype TYMS 1494del6 polymorphism in the Chinese Han population.
authors.affiliation: The National Engineering Research Center for Miniaturized Detection Systems, School of Life Sciences, Northwest University, Xi'an, PR China.
authors.forename: Jin-hui
authors.initials: JH
authors.lastname: Liu
authors.affiliation: The National Engineering Research Center for Miniaturized Detection Systems, School of Life Sciences, Northwest University, Xi'an, PR China.
authors.forename: Ting
authors.initials: T
authors.lastname: Wang
authors.affiliation: The National Engineering Research Center for Miniaturized Detection Systems, School of Life Sciences, Northwest University, Xi'an, PR China.
authors.forename: Wen-jing
authors.initials: WJ
authors.lastname: Zhou
authors.affiliation: The National Engineering Research Center for Miniaturized Detection Systems, School of Life Sciences, Northwest University, Xi'an, PR China.
authors.forename: Cong
authors.initials: C
authors.lastname: Pang
authors.affiliation: The National Engineering Research Center for Miniaturized Detection Systems, School of Life Sciences, Northwest University, Xi'an, PR China; Department of Oncology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, PR China.
authors.forename: Le
authors.initials: L
authors.lastname: Wang
authors.affiliation: The National Engineering Research Center for Miniaturized Detection Systems, School of Life Sciences, Northwest University, Xi'an, PR China.
authors.forename: Chao
authors.initials: C
authors.lastname: Chen
authors.affiliation: The National Engineering Research Center for Miniaturized Detection Systems, School of Life Sciences, Northwest University, Xi'an, PR China. Electronic address: daipg@nuw.edu.cn.
authors.forename: Peng-gao
authors.initials: PG
authors.lastname: Dai
mesh_terms: D000483:Alleles; D044466:Asian People / Q000235:genetics*; D017931:DNA Primers; D060005:Genotyping Techniques / Q000379:methods*; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D013940:Thymidylate Synthase / Q000235:genetics*
chemical_list: D017931:DNA Primers; D013940:Thymidylate Synthase
keywords: Duplex scorpion primers; Genotype; TYMS 1494del6 polymorphism