title: Imaging mass spectrometry of a mouse brain by tapping-mode scanning probe electrospray ionization.
abstract: Methods for ambient sampling and ionization enable chemical information to be obtained with minimal sample preparation. Also, imaging mass spectrometry (IMS) enables the spatial distribution of multiple components to be determined by a single measurement. Here, we report an improved method of tapping-mode scanning probe electrospray ionization (t-SPESI) for ambient sampling and ionization in which probe oscillation is stabilized by using a piezo actuator. We demonstrate negative-mode IMS of a mouse coronal brain section and show that, compared with desorption electrospray ionization, t-SPESI provides unique features in the mass spectra: signal enhancement of fatty acid and lipids, and formation of multivalent ions tentatively assigned to gangliosides. These results would indicate the capability for the generation of multiple types of ions with t-SPESI.
authors.affiliation: Frontier Research Center, Canon Inc., 30-2 Shimomaruko 3-chome, Ohta-ku, Tokyo 146-8501, Japan. otsuka.yoichi@canon.co.jp.
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Otsuka
authors.forename: Junpei
authors.initials: J
authors.lastname: Naito
authors.forename: Shuya
authors.initials: S
authors.lastname: Satoh
authors.forename: Masafumi
authors.initials: M
authors.lastname: Kyogaku
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Hashimoto
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Arakawa
mesh_terms: D000818:Animals; D001921:Brain / Q000033:anatomy & histology*; D051379:Mice; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*