title: [Metabolism of naringin produced by intestinal bacteria].
abstract: Naringin has been reported to possess a wild range of biological activities. However, the route and metabolites of naringin produced by intestinal bacteria are not well understood. In this paper, different bacteria were isolated from human feces and their abilities to convert naringin to different metabolites were studied. Ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) with automated data analysis software (MetaboLynx) was applied to fast analysis of naringin metabolites. Using MSE and mass defect filter techniques, three metabolites were detected and tentatively identified. The results indicated that acetylation, hydrolyzation and hydrolyzation with hydrogenation were the major metabolic pathways of naringin in vitro. Then, we studied the gene sequence of the 16S rRNA of the bacteria by extraction of genomic DNA of the strain, PCR amplification and clone of the 16S rRNA. The consequence proved that Enterococcus sp.30, Bacillus sp.46, Escherichia sp.54 and Escherichia sp.63 have the peculiar metabolism characteristic of naringin.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Shu
authors.initials: S
authors.lastname: Jiang
authors.forename: Da-Wei
authors.initials: DW
authors.lastname: Qian
authors.forename: Er-Xin
authors.initials: EX
authors.lastname: Shang
authors.forename: Ye-Fei
authors.initials: YF
authors.lastname: Qian
authors.forename: Hao
authors.initials: H
authors.lastname: Ren
authors.forename: Han-Liang
authors.initials: HL
authors.lastname: Guan
authors.forename: Jin-Ao
authors.initials: JA
authors.lastname: Duan
mesh_terms: D001407:Bacillus / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D016983:Enterococcus / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D004925:Escherichia / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D005243:Feces / Q000382:microbiology*; D005260:Female; D044950:Flavanones / Q000378:metabolism*; D006801:Humans; D053858:Metabolic Networks and Pathways; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D044950:Flavanones; D012336:RNA, Ribosomal, 16S; C005274:naringin