title: Molecular analysis of Oenococcus oeni and the relationships among and between commercial and autochthonous strains.
abstract: The presence and distribution of genotypes from malolactic starter cultures between the autochthonous microbiota in fermenting Rioja wines have been studied in this paper. The commercial cultures characterization allowed to identify different species and common pulsed field gel electrophoresis and randomly amplified polymorphic DNA genotypes in several brands of Oenococcus oeni starter cultures. Four indistinguishable genotypes were found between the commercial and the autochthonous O. oeni strains. These four genotypes appeared during different vintages in seven of the 10 sampled wineries despite bacterial starter cultures had never been used. Therefore, the detection of commercial LAB genotypes indistinguishable from the autochthonous ones involved their removal from a selection process. These genotypes represented the 17.7% of autochthonous O. oeni isolated in this region during three consecutive years. This fact demonstrated that performing similar studies as previous selection criteria is advisable to avoid large work and the proposal of one commercialized bacteria.
authors.affiliation: ICVV, Instituto de Ciencias de la Vid y del Vino (Gobierno de La Rioja, Universidad de La Rioja and CSIC), C/Madre de Dios 51, 26006 Logroño, La Rioja, Spain.
authors.forename: Lucía
authors.initials: L
authors.lastname: González-Arenzana
authors.affiliation: ICVV, Instituto de Ciencias de la Vid y del Vino (Gobierno de La Rioja, Universidad de La Rioja and CSIC), C/Madre de Dios 51, 26006 Logroño, La Rioja, Spain.
authors.forename: Rosa
authors.initials: R
authors.lastname: López
authors.affiliation: ICVV, Instituto de Ciencias de la Vid y del Vino (Gobierno de La Rioja, Universidad de La Rioja and CSIC), C/Madre de Dios 51, 26006 Logroño, La Rioja, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Portu
authors.affiliation: ICVV, Instituto de Ciencias de la Vid y del Vino (Gobierno de La Rioja, Universidad de La Rioja and CSIC), C/Madre de Dios 51, 26006 Logroño, La Rioja, Spain.
authors.forename: Pilar
authors.initials: P
authors.lastname: Santamaría
authors.affiliation: ICVV, Instituto de Ciencias de la Vid y del Vino (Gobierno de La Rioja, Universidad de La Rioja and CSIC), C/Madre de Dios 51, 26006 Logroño, La Rioja, Spain.
authors.forename: Teresa
authors.initials: T
authors.lastname: Garde-Cerdán
authors.affiliation: ICVV, Instituto de Ciencias de la Vid y del Vino (Gobierno de La Rioja, Universidad de La Rioja and CSIC), C/Madre de Dios 51, 26006 Logroño, La Rioja, Spain. Electronic address: ilopezalfaro@yahoo.es.
authors.forename: Isabel
authors.initials: I
authors.lastname: López-Alfaro
mesh_terms: D015373:Bacterial Typing Techniques; D016521:Electrophoresis, Gel, Pulsed-Field; D005285:Fermentation; D005838:Genotype; D007218:Industrial Microbiology; D056585:Oenococcus / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D014920:Wine / Q000382:microbiology*
keywords: Lactic acid bacteria; Oenococcus oeni; Selection; Starter cultures; Wine