title: Sulphur-containing compounds of durian activate the thermogenesis-inducing receptors TRPA1 and TRPV1.
abstract: Durian (Durio zibethinus Murr.) is classified as a body-warming food in Indian herbalism, and its hyperthermic effect is empirically known in Southeast Asia. To investigate the mechanism underlying this effect, we focused on the thermogenesis-inducing receptors, TRPA1 and TRPV1. Durian contains sulphides similar to the TRPA1 and TRPV1 agonists of garlic. Accordingly, we hypothesized that the thermogenic effect of durian is driven by sulphide-induced TRP channel activation. To investigate our hypothesis, we measured the TRPA1 and TRPV1 activity of the sulphur-containing components of durian and quantified their content in durian pulp. These sulphur-containing components had a stronger effect on TRPA1 than TRPV1. Furthermore, sulphide content in the durian pulp was sufficient to evoke TRP channel activation and the main agonist was diethyl disulphide. From these results, we consider that the body-warming effect of durian is elicited by TRPA1 activation with its sulphides, as can be seen in spices.
authors.affiliation: Graduate School of Pharmaceutical and Nutritional Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka 422-8526, Japan. Electronic address: s12601@u-shizuoka-ken.ac.jp.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Terada
authors.affiliation: Department of Applied Life Sciences, Nihon University Graduate School of Bioresource Sciences, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan. Electronic address: hosono.takashi@nihon-u.ac.jp.
authors.forename: Takashi
authors.initials: T
authors.lastname: Hosono
authors.affiliation: Department of Applied Life Sciences, Nihon University Graduate School of Bioresource Sciences, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan. Electronic address: tseki@brs.nihon-u.ac.jp.
authors.forename: Taiichiro
authors.initials: T
authors.lastname: Seki
authors.affiliation: Department of Applied Life Sciences, Nihon University Graduate School of Bioresource Sciences, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan. Electronic address: ariga@brs.nihon-u.ac.jp.
authors.forename: Toyohiko
authors.initials: T
authors.lastname: Ariga
authors.affiliation: Graduate School of Pharmaceutical and Nutritional Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka 422-8526, Japan. Electronic address: itosohei@u-shizuoka-ken.ac.jp.
authors.forename: Sohei
authors.initials: S
authors.lastname: Ito
authors.affiliation: Graduate School of Pharmaceutical and Nutritional Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka 422-8526, Japan. Electronic address: amstknrk@mail.ecc.u-tokyo.ac.jp.
authors.forename: Masataka
authors.initials: M
authors.lastname: Narukawa
authors.affiliation: Graduate School of Pharmaceutical and Nutritional Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka 422-8526, Japan. Electronic address: watanbt@u-shizuoka-ken.ac.jp.
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Watanabe
mesh_terms: D035161:Bombacaceae / Q000737:chemistry*; D018841:HSP90 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D013455:Sulfur / Q000737:chemistry*; D050916:TRPV Cation Channels / Q000235:genetics / Q000378:metabolism*; D022722:Thermogenesis / Q000502:physiology*
chemical_list: D018841:HSP90 Heat-Shock Proteins; C092306:TRAP1 protein, human; D050916:TRPV Cation Channels; C494482:TRPV1 protein, human; D013455:Sulfur
keywords: Durian; Durio zibethinus Murr.; Sulphur-containing compound; TRPA1; TRPV1; Thermogenesis