title: Explanatory chapter: choosing the right detergent.
abstract: If working with membrane proteins, practically all biochemical techniques can be used as for soluble proteins, the important point being that detergents must be added to keep the membrane proteins in solution. This protocol is meant to help in choosing the right detergent for a given application.
authors.affiliation: Department Protein Evolution, Max-Planck-Institute for Developmental Biology, Tübingen, Germany. Electronic address: dirk.linke@tuebingen.mpg.de.
authors.forename: Dirk
authors.initials: D
authors.lastname: Linke
mesh_terms: D001671:Biochemistry / Q000379:methods*; D002845:Chromatography; D002942:Circular Dichroism; D003460:Crystallization; D003902:Detergents / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008565:Membrane Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D012995:Solubility; D013056:Spectrophotometry, Ultraviolet
chemical_list: D003902:Detergents; D008565:Membrane Proteins
keywords: Affinity chromatography; Detergents; Hydrophobic interaction chromatography (HIC); Ion exchange chromatography; Linear and circular dichroism; Membrane proteins; Polyacrylamide gel electrophoresis; Size exclusion chromatography; Solubilization; UV-VIS spectroscopy