title: Variation in the transmission near-infrared signal with depth in turbid media.
abstract: Transmission near-infrared (NIR) measurements of a 1 mm thick aspirin disk were made at different positions as it was moved through a stack of eight 0.5 mm thick disks of microcrystalline cellulose (Avicel). The magnitude of the first derivative of absorbance for the aspirin interlayer at 8934 cm(-1) was lower when the disk was placed at the top or bottom of the stack of Avicel disks, with the largest signal observed when the aspirin was positioned at the central positions. The variation in signal with depth is consistent with that observed previously for transmission Raman spectrometry. In both cases, the trend observed can be attributed to lower photon density at the air-sample interface, relative to the center of the sample, owing to loss of photons to the air. This results in a reduction in the number of photons absorbed or Raman photons generated and subsequently detected when the interlayer occupies a near-surface position.
authors.affiliation: WestCHEM, Department of Pure and Applied Chemistry and CPACT, University of Strathclyde, 295 Cathedral Street, Glasgow G1 1XL UK.
authors.forename: Nicola
authors.initials: N
authors.lastname: Kellichan
authors.forename: Alison
authors.initials: A
authors.lastname: Nordon
authors.forename: Pavel
authors.initials: P
authors.lastname: Matousek
authors.forename: David
authors.initials: D
authors.lastname: Littlejohn
authors.forename: Gary
authors.initials: G
authors.lastname: McGeorge
mesh_terms: D000042:Absorption; D001241:Aspirin; D002482:Cellulose / Q000737:chemistry; D004304:Dosage Forms; D008956:Models, Chemical; D017785:Photons; D019265:Spectroscopy, Near-Infrared / Q000379:methods*
chemical_list: D004304:Dosage Forms; D002482:Cellulose; D001241:Aspirin