title: A highly selective and sensitive photoswitchable fluorescent probe for Hg2+ based on bisthienylethene-rhodamine 6G dyad and for live cells imaging.
abstract: A new photochromic diarylethene derivative bearing rhodamine 6G dimmer as a fluorescent molecular probe is designed and synthesized successfully. All the compounds are characterized by nuclear magnetic resonance and mass spectrometry. The bisthienylethene-rhodamine 6G dyad exhibit excellent phtochromism with reversibly color and fluorescence changes alternating irradiation with ultraviolet and visible light. Upon addition of Hg(2+), its color changes from colorless to red and its fluorescence is remarkably enhanced. Whereas other ions including K(+), Na(+), Ca(2+), Mg(2+), Fe(2+), Co(2+), Ni(2+), Cu(2+), Zn(2+), Mn(2+), Pb(2+), Ni(2+), Fe(3+), Al(3+), Cr(3+) and so on induce basically no spectral changes, which constitute a highly selective and sensitive photoswitchable fluorescent probe toward Hg(2+). Furthermore, by means of laser confocal scanning microscopy experiments, it is demonstrated that this probe can be applied for live cell imaging and monitoring Hg(2+) in living lung cancer cells with satisfying results, which shows its value of potential application in environmental and biological systems.
authors.affiliation: School of Chemistry and Chemical Engineering, State Key Laboratory of Luminescent Materials and Devices, South China University of Technology, Guangzhou 510641, China; College of Chemistry Science & Technology, Zhanjiang Normal University, Zhanjiang 524048, China.
authors.forename: Li
authors.initials: L
authors.lastname: Xu
authors.affiliation: School of Chemistry and Chemical Engineering, State Key Laboratory of Luminescent Materials and Devices, South China University of Technology, Guangzhou 510641, China; College of Chemistry Science & Technology, Zhanjiang Normal University, Zhanjiang 524048, China; State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China. Electronic address: wang_sheng@ymail.com.
authors.forename: Sheng
authors.initials: S
authors.lastname: Wang
authors.affiliation: Guangdong Key Lab for Research and Development of Natural Drugs, Guangdong Medical College, Zhanjiang 524023, China.
authors.forename: Yingnian
authors.initials: Y
authors.lastname: Lv
authors.affiliation: BK21 FTIT, Department of Organic Materials and Textile System Engineering, Chungnam National University, Daejeon 305-764, South Korea.
authors.forename: Young-A
authors.initials: YA
authors.lastname: Son
authors.affiliation: School of Chemistry and Chemical Engineering, State Key Laboratory of Luminescent Materials and Devices, South China University of Technology, Guangzhou 510641, China. Electronic address: drcao@scut.edu.cn.
authors.forename: Derong
authors.initials: D
authors.lastname: Cao
mesh_terms: D045744:Cell Line, Tumor; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism* / Q000473:pathology*; D008628:Mercury / Q000032:analysis* / Q000378:metabolism; D008856:Microscopy, Fluorescence / Q000379:methods; D012235:Rhodamines / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D012235:Rhodamines; C026188:rhodamine 6G; D008628:Mercury
keywords: Biological imaging; Bisthienylethene; Fluorescence; Hg(2+) probe; Photochromism; Rhodamine