title: Direct visualization of CHIP-mediated degradation of alpha-synuclein in vivo: implications for PD therapeutics.
abstract: Parkinson's disease is a neurodegenerative disorder characterized by Lewy bodies, a pathological hallmark comprised mostly of aggregated alpha synuclein. Accumulating evidence demonstrates the association of smaller oligomeric aggregates to disease etiology and many therapeutic approaches are aimed at inhibiting and reducing the aggregation process. Molecular chaperones and co-chaperones play a key role in protein homeostasis and have potential as therapeutics to inhibit alpha synuclein associated toxicity. Here we use a gene therapy approach to evaluate the applicability of the Hsp70 co-chaperone CHIP (C-terminal Hsp70 interacting protein) as a therapeutic candidate and examine its direct effect on alpha synuclein aggregates in vivo. Utilizing a novel viral vector mediated rat model to directly detect alpha synuclein aggregates, we show that CHIP can mediate the degradation of alpha synuclein aggregates in vivo. However, our studies also reveal that CHIP may potentially degrade tyrosine hydroxylase which would compromise the applicability of CHIP as a therapeutic approach for Parkinson's disease.
authors.affiliation: MassGeneral Institute for Neurodegenerative Disease, Department of Neurology, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts, United States of America.
authors.forename: Hemi
authors.initials: H
authors.lastname: Dimant
authors.affiliation: MassGeneral Institute for Neurodegenerative Disease, Department of Neurology, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts, United States of America.
authors.forename: Liya
authors.initials: L
authors.lastname: Zhu
authors.affiliation: MassGeneral Institute for Neurodegenerative Disease, Department of Neurology, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts, United States of America.
authors.forename: Laura N
authors.initials: LN
authors.lastname: Kibuuka
authors.affiliation: MassGeneral Institute for Neurodegenerative Disease, Department of Neurology, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts, United States of America.
authors.forename: Zhanyun
authors.initials: Z
authors.lastname: Fan
authors.affiliation: MassGeneral Institute for Neurodegenerative Disease, Department of Neurology, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts, United States of America.
authors.forename: Bradley T
authors.initials: BT
authors.lastname: Hyman
authors.affiliation: MassGeneral Institute for Neurodegenerative Disease, Department of Neurology, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts, United States of America; Department of Neuroscience, Mayo Clinic, Jacksonville, Florida, United States of America.
authors.forename: Pamela J
authors.initials: PJ
authors.lastname: McLean
mesh_terms: D000818:Animals; D057054:Molecular Imaging; D010300:Parkinson Disease / Q000378:metabolism / Q000628:therapy*; D055503:Protein Multimerization; D017433:Protein Structure, Secondary; D059748:Proteolysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism; D044767:Ubiquitin-Protein Ligases / Q000378:metabolism*; D051844:alpha-Synuclein / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D051844:alpha-Synuclein; D014446:Tyrosine 3-Monooxygenase; C540442:Stub1 protein, rat; D044767:Ubiquitin-Protein Ligases