title: Identification of human tissue kallikrein 6 as a potential marker of laryngeal cancer based on the relevant secretory/releasing protein database.
abstract: OBJECTIVE
  This study was aimed to create a large-scale laryngeal cancer relevant secretory/releasing protein database and further discover candidate biomarkers.
  
  
  METHODS
  Primary tissue cultures were established using tumor tissues and matched normal mucosal tissues collected from four laryngeal cancer patients. Serum-free conditioned medium (CM) samples were collected. These samples were then sequentially processed by SDS-PAGE separation, trypsin digestion, and LC-MS/MS analysis. The candidates in the database were validated by ELISA using plasma samples from laryngeal cancer patients, benign patients, and healthy individuals.
  
  
  RESULTS
  Combining MS data from the tumor tissues and normal tissues, 982 proteins were identified in total; extracellular proteins and cell surface proteins accounted for 15.0% and 4.3%, respectively. According to stringent criteria, 49 proteins were selected as candidates worthy of further validation. Of these, human tissue kallikrein 6 (KLK6) was verified. The level of KLK6 was significantly increased in the plasma samples from the cancer cohort compared to the benign and healthy cohorts and moreover showed a slight decrease in the postoperative plasma samples in comparison to the preoperative plasma samples.
  
  
  CONCLUSIONS
  This laryngeal cancer-derived protein database provides a promising repository of candidate blood biomarkers for laryngeal cancer. The diagnostic potential of KLK6 deserves further investigation.
authors.affiliation: Department of Biochemistry and Molecular Biology, Molecular Medicine and Cancer Research Center, Chongqing Medical University, Chongqing 400016, China ; State Key Laboratory of Molecular Oncology, Beijing Key Laboratory for Carcinogenesis and Cancer Prevention, Cancer Institute (Hospital), Peking Union Medical College & Chinese Academy of Medical Sciences, P.O. Box 2258, Beijing 100021, China.
authors.forename: Ying
authors.identifier: 0000-0002-5414-6617
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Head and Neck Surgical Oncology, Cancer Institute (Hospital), Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100021, China.
authors.forename: Zaixing
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Molecular Oncology, Beijing Key Laboratory for Carcinogenesis and Cancer Prevention, Cancer Institute (Hospital), Peking Union Medical College & Chinese Academy of Medical Sciences, P.O. Box 2258, Beijing 100021, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Yang
authors.affiliation: National Center of Biomedical Analysis, Beijing 100021, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Xu
authors.affiliation: National Center of Biomedical Analysis, Beijing 100021, China.
authors.forename: Weihua
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Head and Neck Surgical Oncology, Cancer Institute (Hospital), Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100021, China.
authors.forename: Pingzhang
authors.initials: P
authors.lastname: Tang
authors.affiliation: Department of Head and Neck Surgical Oncology, Cancer Institute (Hospital), Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100021, China.
authors.forename: Zongmin
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Molecular Oncology, Beijing Key Laboratory for Carcinogenesis and Cancer Prevention, Cancer Institute (Hospital), Peking Union Medical College & Chinese Academy of Medical Sciences, P.O. Box 2258, Beijing 100021, China.
authors.forename: Naijun
authors.initials: N
authors.lastname: Han
authors.affiliation: State Key Laboratory of Molecular Oncology, Beijing Key Laboratory for Carcinogenesis and Cancer Prevention, Cancer Institute (Hospital), Peking Union Medical College & Chinese Academy of Medical Sciences, P.O. Box 2258, Beijing 100021, China.
authors.forename: Yanning
authors.initials: Y
authors.lastname: Gao
authors.affiliation: State Key Laboratory of Molecular Oncology, Beijing Key Laboratory for Carcinogenesis and Cancer Prevention, Cancer Institute (Hospital), Peking Union Medical College & Chinese Academy of Medical Sciences, P.O. Box 2258, Beijing 100021, China.
authors.forename: Shujun
authors.initials: S
authors.lastname: Cheng
authors.affiliation: State Key Laboratory of Molecular Oncology, Beijing Key Laboratory for Carcinogenesis and Cancer Prevention, Cancer Institute (Hospital), Peking Union Medical College & Chinese Academy of Medical Sciences, P.O. Box 2258, Beijing 100021, China.
authors.forename: Ting
authors.identifier: 0000-0002-9000-1909
authors.initials: T
authors.lastname: Xiao
mesh_terms: D014408:Biomarkers, Tumor / Q000097:blood*; D002294:Carcinoma, Squamous Cell / Q000097:blood* / Q000601:surgery; D016022:Case-Control Studies; D030562:Databases, Protein; D005260:Female; D006801:Humans; D007610:Kallikreins / Q000097:blood*; D007822:Laryngeal Neoplasms / Q000097:blood* / Q000601:surgery; D008297:Male; D008875:Middle Aged; D046509:Tissue Culture Techniques
chemical_list: D014408:Biomarkers, Tumor; C103551:KLK6 protein, human; D007610:Kallikreins