title: Transcriptomic study of 39 ostreid herpesvirus 1 genes during an experimental infection.
abstract: Massive mortality outbreaks have been reported in France since 2008 among Pacific oysters, Crassostrea gigas, with the detection of a particular OsHV-1 variant called μVar. Virus infection can be induced in healthy spat in experimental conditions allowing to better understand the disease process, including viral gene expression. Although gene expression of other herpesviruses has been widely studied, we provide the first study following viral gene expression of OsHV-1 over time. In this context, an in vivo transcriptomic study targeting 39 OsHV-1 genes was carried out during an experimental infection of Pacific oyster spat. For the first time, several OsHV-1 mRNAs were detected by real-time PCR at 0 h, 2 h, 4 h, 18 h, 26 h and 42 h post-injection. Several transcripts were detected at 2h post-infection and at 18 h post-infection for all selected ORFs. Quantification of virus gene expression at different times of infection was also carried out using an oyster housekeeping gene, Elongation factor. Developing an OsHV-1-specific reverse transcriptase real time PCR targeting 39 viral gene appears a new tool in terms of diagnosis and can be used to complement viral DNA detection in order to monitor viral replication.
authors.affiliation: Ifremer (Institut Français de Recherche pour l'Exploitation de la Mer), Unité Santé, Génétique et Microbiologie des Mollusques (SG2M), Laboratoire de Génétique et Pathologie des Mollusques Marins (LGPMM), Avenue de Mus de Loup, 17390 La Tremblade, France.
authors.forename: Amélie
authors.initials: A
authors.lastname: Segarra
authors.affiliation: Ifremer (Institut Français de Recherche pour l'Exploitation de la Mer), Unité Santé, Génétique et Microbiologie des Mollusques (SG2M), Laboratoire de Génétique et Pathologie des Mollusques Marins (LGPMM), Avenue de Mus de Loup, 17390 La Tremblade, France.
authors.forename: Nicole
authors.initials: N
authors.lastname: Faury
authors.affiliation: Ifremer, Laboratoire Environnement Ressources des Pertuis Charentais (LERPC), Avenue de Mus de Loup, 17390 La Tremblade, France.
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Pépin
authors.affiliation: Ifremer (Institut Français de Recherche pour l'Exploitation de la Mer), Unité Santé, Génétique et Microbiologie des Mollusques (SG2M), Laboratoire de Génétique et Pathologie des Mollusques Marins (LGPMM), Avenue de Mus de Loup, 17390 La Tremblade, France. Electronic address: Tristan.Renault@ifremer.fr.
authors.forename: Tristan
authors.initials: T
authors.lastname: Renault
mesh_terms: D000818:Animals; D049896:Crassostrea / Q000821:virology*; D006564:Herpesviridae / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D059467:Transcriptome
chemical_list: D012367:RNA, Viral
keywords: Crassostrea gigas; Elongation factor; Inhibitors of apoptosis; OsHV-1; Real time PCR; Viral gene expression