title: Two-signal electrochemical method for evaluation suppression and proliferation of MCF-7 cells based on intracellular purine.
abstract: Two electrochemical signals ascribed to xanthine/guanine and hypanthine/adenine in MCF-7 cells were detected at 0.726 and 1.053 V, respectively. Based on the intensity of signals, the genistein-induced proliferation and suppression of MCF-7 cells could be evaluated. The results showed that with the increase of genistein dose at the range of 10(-9) to 10(-6)M, the two electrochemical signals of MCF-7 cell suspension increased due to the proliferation, whereas the tendency at the high dosage range of more than 10(-5)M was decreased. The proliferation and cytotoxicity obtained by the electrochemical method were in agreement with those obtained by cell counting and the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium] method. Thus, the two-signal electrochemical method is an effective way to evaluate the effect of drugs on cell activity based on purine metabolism.
authors.affiliation: College of Pharmacy, Jiamusi University, Jiamusi 154007, China.
authors.forename: Jinlian
authors.initials: J
authors.lastname: Li
authors.affiliation: College of Pharmacy, Jiamusi University, Jiamusi 154007, China.
authors.forename: Runxian
authors.initials: R
authors.lastname: Lin
authors.affiliation: College of Pharmacy, Jiamusi University, Jiamusi 154007, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.affiliation: College of Pharmacy, Jiamusi University, Jiamusi 154007, China.
authors.forename: Guanggang
authors.initials: G
authors.lastname: Gao
authors.affiliation: College of Pharmacy, Jiamusi University, Jiamusi 154007, China.
authors.forename: Jiwen
authors.initials: J
authors.lastname: Cui
authors.affiliation: School of Stomatology, Jiamusi University, Jiamusi 154007, China. Electronic address: liujg5550@163.com.
authors.forename: Jiguang
authors.initials: J
authors.lastname: Liu
authors.affiliation: College of Pharmacy, Jiamusi University, Jiamusi 154007, China. Electronic address: wudmei67@163.com.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Wu
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor / Q000295:instrumentation / Q000379:methods*; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D019833:Genistein / Q000494:pharmacology*; D006801:Humans; D042541:Intracellular Space / Q000187:drug effects / Q000378:metabolism*; D061986:MCF-7 Cells; D037742:Nanotubes, Carbon / Q000737:chemistry; D011687:Purines / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000970:Antineoplastic Agents; D037742:Nanotubes, Carbon; D011687:Purines; D019833:Genistein; C030985:purine
keywords: Cell activity; Electrochemistry; Genistein; MCF-7 cells