title: Establishment and characterization of an ovarian cell line from Southern catfish (Silurus meridionalis).
abstract: An ovarian cell line was successfully developed from the juvenile ovary of Southern catfish (SCO1) (Silurus meridionalis), which was designated as SCO1. The cell line multiplied preferentially in L-15 medium with 15 % fetal bovine serum at 28 °C for more than 70 passages over a period of 420 days. SCO1 showed fibroblast-like morphology and predominantly retained a diploid karyotype of 58 chromosomes. From the gene expression patterns, SCO1 showed a characteristic of ovarian granulosa cells. After the cells were transfected with the green fluorescent protein expression vector, bright fluorescent signals could be observed in approximately 30 % cells. This cell line may be valuable for the evaluation of endocrine disruptors and studying interactions between somatic cells and germ cells.
authors.affiliation: Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Southwest University, Chongqing, 400715, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wei
authors.forename: WenChuang
authors.initials: W
authors.lastname: Qi
authors.forename: Yujie
authors.initials: Y
authors.lastname: Zhou
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Zhang
authors.forename: Ranran
authors.initials: R
authors.lastname: Dong
authors.forename: Linyan
authors.initials: L
authors.lastname: Zhou
authors.forename: Deshou
authors.initials: D
authors.lastname: Wang
mesh_terms: D000818:Animals; D002397:Catfishes; D002460:Cell Line; D049109:Cell Proliferation / Q000502:physiology; D017931:DNA Primers / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling / Q000662:veterinary; D005822:Genetic Vectors / Q000235:genetics; D006107:Granulosa Cells / Q000166:cytology*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D007621:Karyotyping / Q000662:veterinary; D010053:Ovary / Q000166:cytology*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary
chemical_list: D017931:DNA Primers; D049452:Green Fluorescent Proteins