title: Solid phase microextraction coupled to liquid chromatography. Analysis of organosulphur compounds avoiding artifacts formation.
abstract: This work proposes the novel application of a microextraction technique, solid phase microextraction (SPME), coupled to liquid chromatography with UV detection (HPLC-UV) for the analysis of organosulfur compounds (OSCs) in garlic samples. Additionally, a comparative study of OSCs profiles obtained by SPME coupled to HPLC-UV and gas chromatography with flame photometric detector (GC-FPD), respectively; was carried out. This study provided complementary evidence about OSCs's lability and "artifacts" formation during the analytical process. Raw, cooked and distilled garlic samples were considered. The target analytes were diallyl disulphide (DADS), diallyl sulphide (DAS), diallyl trisulphide (DATS), allicin, 3-vinyl-4H-1,3-dithiin (3-VD), 2-vinyl-4H-1,2-dithiin (2-VD) and (E)- and (Z)-ajoene, which are the most important OSCs with biological activities present in raw and processed garlic. The coupling of SPME and HPLC showed to be reliable, fast, sensible and selective methodology for OSCs analysis.
authors.affiliation: Instituto de Biología Agrícola de Mendoza (IBAM) - CONICET, Mendoza, Argentina; Facultad de Ciencias Agrarias, Universidad Nacional de Cuyo, Alte. Brown 500, 5505 Chacras de Coria, Mendoza, Argentina.
authors.forename: Daniela A
authors.initials: DA
authors.lastname: Locatelli
authors.affiliation: Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales (IANIGLA) - CONICET, Mendoza, Argentina; Instituto de Ciencias Básicas, Universidad Nacional de Cuyo, Mendoza, Argentina.
authors.forename: Jorgelina C
authors.initials: JC
authors.lastname: Altamirano
authors.affiliation: Facultad de Química Bioquímica y Farmacia, Universidad Nacional de San Luis, Chacabuco y Pedernera, San Luis, Argentina.
authors.forename: Juan M
authors.initials: JM
authors.lastname: Luco
authors.affiliation: Swedish Defence Research Agency, CBRN Defence and Security, Umeå, Sweden.
authors.forename: Rikard
authors.initials: R
authors.lastname: Norlin
authors.affiliation: Instituto de Biología Agrícola de Mendoza (IBAM) - CONICET, Mendoza, Argentina; Facultad de Ciencias Agrarias, Universidad Nacional de Cuyo, Alte. Brown 500, 5505 Chacras de Coria, Mendoza, Argentina; Instituto de Ciencias Básicas, Universidad Nacional de Cuyo, Mendoza, Argentina. Electronic address: acamargo@fca.uncu.edu.ar.
authors.forename: Alejandra B
authors.initials: AB
authors.lastname: Camargo
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002853:Chromatography, Liquid; D005737:Garlic / Q000737:chemistry; D052617:Solid Phase Microextraction / Q000379:methods*
keywords: Artifacts; GC; Garlic; HPLC; Organosulphur compounds; SPME