title: Differential expression of the main polycyclic aromatic hydrocarbon responsive genes in the extrahepatic tissues of mice.
abstract: The hepatic toxic effects, including carcinogenicity and oxidative stress, of polycyclic aromatic hydrocarbons (PAHs) have been extensively studied in recent years. Previous reports have demonstrated that 3-methylcholanthrene (3MC) is capable of altering the expression of aryl hydrocarbon receptor (AHR)-regulated genes and antioxidant genes in liver, but little is known about the expression patterns in other tissues. To investigate whether similar effects could occur in the extrahepatic tissues, adult male ICR mice were received an intraperitoneal injection of 100 mg/kg 3MC and then analyzed after 6 and 24 h. We observed that the constitutive expression of AHR- and antioxidant-related genes was in a tissue-specific manner. Moreover, acute 3MC exposure significantly increased the mRNA levels of Cyp1a1 and Cyp1b1 in all the lung, kidney and heart. As to antioxidant genes, 3MC induced the transcription of glutathione reductase (Gr) in the lung and kidney at 24 h and the transcription of glutathione peroxidase 1 (Gpx1) in the lung and kidney at 6 and 24 h. Glutathione-S-transferase A1 (Gsta1) was significantly reduced in the kidney at 24 h, while no effect was observed in the lung and heart. The mRNA levels of
  quinone oxidoreductase 1 (Nqo1) were induced by 3MC in all the lung, kidney and heart. Although the constitutive expression of catalase (Cat) is very low in the heart, the transcription of Cat was significantly induced both at 6 and 24 h. No significant alternation in the transcription of glutathione synthetase (Gss), heme oxygenase 1 (Ho-1) and superoxide dismutase 1 (Sod1) was observed in all tissues. Taken together, ours findings suggested that the expression of AHR- and antioxidant-related genes in a tissue-specific manner with or without treatment of a PAH.
authors.affiliation: College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, China.
authors.forename: Wenyu
authors.initials: W
authors.lastname: Miao
authors.affiliation: College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, China.
authors.forename: Yuanxiang
authors.initials: Y
authors.lastname: Jin
authors.affiliation: College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, China.
authors.forename: Xiaojian
authors.initials: X
authors.lastname: Lin
authors.affiliation: College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, China. Electronic address: azwfu@zjut.edu.cn.
authors.forename: Zhengwei
authors.initials: Z
authors.lastname: Fu
mesh_terms: D000818:Animals; D002374:Catalase / Q000235:genetics; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics; D065633:Cytochrome P-450 CYP1B1 / Q000235:genetics; D004785:Environmental Pollutants / Q000633:toxicity*; D015870:Gene Expression / Q000187:drug effects*; D005979:Glutathione Peroxidase / Q000235:genetics; D005980:Glutathione Reductase / Q000235:genetics; D005981:Glutathione Synthase / Q000235:genetics; D005982:Glutathione Transferase / Q000235:genetics; D024581:Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) / Q000235:genetics; D051547:Heme Oxygenase-1 / Q000235:genetics; D007527:Isoenzymes / Q000235:genetics; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism; D008168:Lung / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000235:genetics; D008748:Methylcholanthrene / Q000633:toxicity*; D008813:Mice, Inbred ICR; D009206:Myocardium / Q000378:metabolism*; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D018336:Receptors, Aryl Hydrocarbon / Q000378:metabolism; D013482:Superoxide Dismutase / Q000235:genetics; D000072105:Superoxide Dismutase-1; D000094765:Glutathione Peroxidase GPX1; D000099156:Superoxide Dismutase 2
chemical_list: D002374:Catalase; D019363:Cytochrome P-450 CYP1A1; D065633:Cytochrome P-450 CYP1B1; D004785:Environmental Pollutants; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D005981:Glutathione Synthase; D005982:Glutathione Transferase; D024581:Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating); D051547:Heme Oxygenase-1; D007527:Isoenzymes; D008565:Membrane Proteins; D008748:Methylcholanthrene; D016660:NAD(P)H Dehydrogenase (Quinone); D012333:RNA, Messenger; D018336:Receptors, Aryl Hydrocarbon; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1; D000094765:Glutathione Peroxidase GPX1; D000099156:Superoxide Dismutase 2; C585498:Cyp1b1 protein, mouse; C496542:Hmox1 protein, mouse; C000606291:Sod1 protein, mouse; C491732:Nqo1 protein, mouse; C119995:glutathione S-transferase alpha; C000722068:Gpx1 protein, mouse
keywords: 3MC; AHR-regulated genes; Antioxidant genes; Tissue-specific