title: Soluble VEGFR1 concentration in the serum of patients with colorectal cancer.
abstract: OBJECTIVE
  VEGFR is involved in complex biological processes, including inflammation and cancer development, progression and metastasis. Many proteins, including VEGFR, are proteolytically released from the surface of cells by a process known as ectodomain shedding. The aim of this study was to assess the expression of soluble VEGFR1 (sVEGFR1) in the serum of patients with colorectal cancer (CRC).
  
  
  METHODS
  Sixty-two serum samples from healthy controls and 88 samples from patients with different stages of CRC were included in this study. The total protein concentration (TPC) was measured using a Bio-Rad protein assay, and the expression and concentration of sVEGFR1 was determined by a Western blot analysis and enzyme-linked immunosorbent assay, respectively.
  
  
  RESULTS
  No significant difference in the serum TPC of patients with and without CRC was seen. The relative s-VEGFR1 expression and concentration of sVEGFR1 in the serum of patients with CRC were significantly increased compared to those in controls (P < 0.001).
  
  
  CONCLUSIONS
  The results of this study suggest that VEGFR1 shedding may provide a reliable and practical indicator of the malignant potential, tumor progression and overall tumor burden. The findings also suggest that sVEGFR1 might be involved in the pathophysiology of CRC, and the detection of serum sVEGFR1 may be useful in classifying CRC.
authors.affiliation: Department of Cell and Molecular Biology, Pardis International, University of Guilan, Rasht, Iran.
authors.forename: Oranus
authors.initials: O
authors.lastname: Abbasi
authors.forename: Farhad
authors.initials: F
authors.lastname: Mashayekhi
authors.forename: Ebrahim
authors.initials: E
authors.lastname: Mirzajani
authors.forename: Saba
authors.initials: S
authors.lastname: Fakhriyeh Asl
authors.forename: Touraj
authors.initials: T
authors.lastname: Mahmoudi
authors.forename: Hamid
authors.initials: H
authors.lastname: Saeedi Saedi
mesh_terms: D014408:Biomarkers, Tumor / Q000097:blood*; D015179:Colorectal Neoplasms / Q000175:diagnosis / Q000473:pathology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D012995:Solubility; D040281:Vascular Endothelial Growth Factor Receptor-1 / Q000097:blood*
chemical_list: D014408:Biomarkers, Tumor; D040281:Vascular Endothelial Growth Factor Receptor-1