title: Identification and characterization of MT-1X as a novel FHL3-binding partner.
abstract: Four and a half LIM domain protein 3 (FHL3) is a member of the FHL protein family that plays roles in the regulation of cell survival, cell adhesion and signal transduction. However, the mechanism of action for FHL3 is not yet clear. The aim of present study was to identify novel binding partner of FHL3 and to explore the underlying mechanism. With the use of yeast two-hybrid screening system, FHL3 was used as the bait to screen human fetal hepatic cDNA library for interacting proteins. Methionine-1X was identified as a novel FHL3 binding partner. The interaction between FHL3 and the full length MT-1X was further confirmed by yeast two-hybrid assay, co-immunoprecipitation and GST pull-down assays. Furthermore,the result demonstrated that MT-1X knockdown promoted the FHL3-induced inhibitory effect on HepG2 cells by regulating FHL3-mediated Smad signaling and involving in the modulation the expression of G2/M phase-related proteins through interaction with FHL3. These findings suggest that functional interactions between FHL3 and MT-1X may provide some clues to the mechanisms of FHL3-regulated cell proliferation.
authors.affiliation: Laboratory of Genome Engineering, Beijing Institute of Basic Medical Sciences, Beijing, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Cai
authors.affiliation: Laboratory of Genome Engineering, Beijing Institute of Basic Medical Sciences, Beijing, PR China.
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Wang
authors.affiliation: Laboratory of Genome Engineering, Beijing Institute of Basic Medical Sciences, Beijing, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Huang
authors.affiliation: Laboratory of Genome Engineering, Beijing Institute of Basic Medical Sciences, Beijing, PR China.
authors.forename: Wenliang
authors.initials: W
authors.lastname: Fu
authors.affiliation: Laboratory of Genome Engineering, Beijing Institute of Basic Medical Sciences, Beijing, PR China.
authors.forename: Wenrong
authors.initials: W
authors.lastname: Xia
authors.affiliation: Laboratory of Genome Engineering, Beijing Institute of Basic Medical Sciences, Beijing, PR China.
authors.forename: Minji
authors.initials: M
authors.lastname: Zou
authors.affiliation: Laboratory of Genome Engineering, Beijing Institute of Basic Medical Sciences, Beijing, PR China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Laboratory of Genome Engineering, Beijing Institute of Basic Medical Sciences, Beijing, PR China.
authors.forename: Jiaxi
authors.initials: J
authors.lastname: Wang
authors.affiliation: Laboratory of Genome Engineering, Beijing Institute of Basic Medical Sciences, Beijing, PR China.
authors.forename: Donggang
authors.initials: D
authors.lastname: Xu
mesh_terms: D002448:Cell Adhesion / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics*; D005786:Gene Expression Regulation; D055785:Gene Knockdown Techniques; D015723:Gene Library; D056945:Hep G2 Cells; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D060588:LIM Domain Proteins / Q000235:genetics / Q000378:metabolism*; D008668:Metallothionein / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D011485:Protein Binding; D015398:Signal Transduction / Q000235:genetics
chemical_list: C117563:FHL3 protein, human; D047908:Intracellular Signaling Peptides and Proteins; D060588:LIM Domain Proteins; C407523:metallothionein isoform 1; D008668:Metallothionein