title: Biochemical analysis of metallo-β-lactamase NDM-3 from a multidrug-resistant Escherichia coli strain isolated in Japan.
abstract: New Delhi metallo-β-lactamase-3 (NDM-3) was identified in a multidrug-resistant Escherichia coli isolate, NCGM77, obtained from the feces of a patient in Japan. The enzymatic activities of NDM-3 against β-lactams were similar to those of NDM-1, although NDM-3 showed slightly lower kcat/Km ratios for all the β-lactams tested except for doripenem. The genetic context for blaNDM-3 was tnpA-blaNDM-3-bleMBL-trpF-dsbC-tnpA-sulI-qacEdeltaI-aadA2-dfrA1, which was present on an approximately 250-kb plasmid.
authors.affiliation: Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, Toyama, Shinjuku, Tokyo, Japan.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Tada
authors.affiliation: Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, Toyama, Shinjuku, Tokyo, Japan.
authors.forename: Tohru
authors.initials: T
authors.lastname: Miyoshi-Akiyama
authors.affiliation: Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, Toyama, Shinjuku, Tokyo, Japan.
authors.forename: Kayo
authors.initials: K
authors.lastname: Shimada
authors.affiliation: Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, Toyama, Shinjuku, Tokyo, Japan tkirikae@ri.imcj.go.jp.
authors.forename: Teruo
authors.initials: T
authors.lastname: Kirikae
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D015780:Carbapenems / Q000494:pharmacology; D000077726:Doripenem; D024901:Drug Resistance, Multiple, Bacterial; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D004927:Escherichia coli Infections / Q000382:microbiology*; D006801:Humans; D007564:Japan; D008826:Microbial Sensitivity Tests; D010957:Plasmids / Q000187:drug effects; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*; D047090:beta-Lactams / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D015780:Carbapenems; D047090:beta-Lactams; D000077726:Doripenem; D001618:beta-Lactamases; C552864:beta-lactamase NDM-1