title: Osteoinductive activity of ErhBMP-2 after anterior cervical diskectomy and fusion with a ß-TCP interbody cage in a goat model.
abstract: Bone morphogenetic protein (BMP)-2 induces bone and cartilage tissue formation. Large amounts of BMP-2 are difficult to purify or to produce in vitro using eukaryotic cells. The goal of the present study was to assess the clinical use of Escherichia coli-derived recombinant human BMP-2 (ErhBMP-2) on bone fusion after cervical and lumbar spine surgery in a goat model, compared with the standard autogenous iliac bone grafting. Thirty-six goats were randomized to 3 groups: (A) autogenous iliac bone grafting, (B) cervical interbody fusion cage containing ß-tricalcium phosphate (ß-TCP), or (C) cervical interbody fusion cage containing ß-TCP+ErhBMP-2 (2.5 mg). Cervical bone repair was evaluated using radiographs and computed tomography scans at 0, 3, and 6 months. Histological analyses were performed on cervical samples. Two goats died from infection. The differences in intervertebral height among the groups were not significant 3 months postoperatively but became significant after 6 months between groups A vs B and C (P=.04); there was no difference between groups B and C at 6 months. Adding ErhBMP-2 significantly increased cervical fusion at 6 months (P=.04). Histological examinations showed that ß-TCP+ErhBMP-2 increased new bone area, material degradation rate, and depth of tissue penetration and decreased residual material area, all in a time-dependent manner. Escherichia coli-derived rhBMP-2 combined with an enhanced fusion cage containing ß-TCP induced bone formation in a goat model. Furthermore, its ability to promote bone fusion was similar to autogenous iliac bone grafting.
authors.forename: Hongli
authors.initials: H
authors.lastname: Wang
authors.forename: Fan
authors.initials: F
authors.lastname: Zhang
authors.forename: Feizhou
authors.initials: F
authors.lastname: Lv
authors.forename: Jianyuan
authors.initials: J
authors.lastname: Jiang
authors.forename: Dayong
authors.initials: D
authors.lastname: Liu
authors.forename: Xinlei
authors.initials: X
authors.lastname: Xia
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000008:administration & dosage* / Q000235:genetics; D001860:Bone Plates; D018786:Bone Substitutes; D002130:Calcium Phosphates / Q000008:administration & dosage*; D002574:Cervical Vertebrae / Q000000981:diagnostic imaging* / Q000601:surgery*; D003131:Combined Modality Therapy; D004195:Disease Models, Animal; D017586:Diskectomy / Q000295:instrumentation*; D004343:Drug Implants / Q000008:administration & dosage; D004359:Drug Therapy, Combination / Q000379:methods; D006041:Goats; D008297:Male; D010012:Osteogenesis / Q000187:drug effects; D011859:Radiography; D011897:Random Allocation; D011994:Recombinant Proteins / Q000008:administration & dosage; D013123:Spinal Fusion / Q000295:instrumentation*; D016896:Treatment Outcome
chemical_list: C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D018786:Bone Substitutes; D002130:Calcium Phosphates; D004343:Drug Implants; D011994:Recombinant Proteins; C485817:beta-tricalcium phosphate