title: ORC1/CDC6 and MCM7 distinct associate with chromatin through Trypanosoma cruzi life cycle.
abstract: Trypanosoma cruzi alternates between replicative and non-replicative stages. We analyzed the expression of components of the pre-replication machinery TcORC1/CDC6 and TcMCM7 and their interaction with DNA in all T. cruzi stages. TcORC1/CDC6 remains in the nuclear space during all stages of the life cycle and interacts with DNA in the replicative stages; however, it does not bind to DNA in the non-replicative forms. Moreover, TcMCM7 is not present in the non-replicative stages. These data suggest that the lacking of DNA replication during the T. cruzi life cycle may be a consequence of the blocking of TcORC1/CDC6-DNA interaction and of the down regulation of the TcMCM7 expression.
authors.affiliation: Laboratório Especial de Ciclo Celular, Instituto Butantan, São Paulo, Brazil; Center of Toxins, Immune Response and Cell Signaling - CeTICS, Brazil.
authors.forename: Simone
authors.initials: S
authors.lastname: Calderano
authors.affiliation: Laboratório Especial de Ciclo Celular, Instituto Butantan, São Paulo, Brazil.
authors.forename: Patricia
authors.initials: P
authors.lastname: Godoy
authors.affiliation: Laboratório Especial de Ciclo Celular, Instituto Butantan, São Paulo, Brazil; Center of Toxins, Immune Response and Cell Signaling - CeTICS, Brazil.
authors.forename: Daiane
authors.initials: D
authors.lastname: Soares
authors.affiliation: Laboratório de Imunoquímica, Instituto Butantan, São Paulo, Brazil; Center of Toxins, Immune Response and Cell Signaling - CeTICS, Brazil.
authors.forename: Osvaldo Augusto
authors.initials: OA
authors.lastname: Sant'Anna
authors.affiliation: Departamento de Micro, Imuno e Parasitologia, Universidade Federal de São Paulo, Brazil.
authors.forename: Sergio
authors.initials: S
authors.lastname: Schenkman
authors.affiliation: Laboratório Especial de Ciclo Celular, Instituto Butantan, São Paulo, Brazil; Center of Toxins, Immune Response and Cell Signaling - CeTICS, Brazil. Electronic address: carolina.eliassabbaga@butantan.gov.br.
authors.forename: M Carolina
authors.initials: MC
authors.lastname: Elias
mesh_terms: D000818:Animals; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D002843:Chromatin / Q000378:metabolism*; D004261:DNA Replication; D008018:Life Cycle Stages; D064168:Minichromosome Maintenance Complex Component 7 / Q000235:genetics / Q000378:metabolism*; D051738:Origin Recognition Complex / Q000235:genetics / Q000378:metabolism*; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism; D014349:Trypanosoma cruzi / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism
chemical_list: D018797:Cell Cycle Proteins; D002843:Chromatin; D051738:Origin Recognition Complex; D015800:Protozoan Proteins; D064168:Minichromosome Maintenance Complex Component 7
keywords: DNA replication; MCM7; ORC1/CDC6; Trypanosoma cruzi