title: Suppression of elongation and growth of tomato seedlings by auxin biosynthesis inhibitors and modeling of the growth and environmental response.
abstract: To develop a growth inhibitor, the effects of auxin inhibitors were investigated. Application of 30 μM L-α-aminooxy-β-phenylpropionic acid (AOPP) or (S)-methyl 2-((1,3-dioxoisoindolin-2-yl)oxy)-3-phenylpropanoate (KOK1101), decreased the endogenous IAA levels in tomato seedlings at 8 days after sowing. Then, 10-1200 μM AOPP or KOK1101 were sprayed on the leaves and stem of 2-3 leaf stage tomato plants grown under a range of environmental conditions. We predicted plant growth and environmental response using a model based on the observed suppression of leaf enlargement. Spraying AOPP or KOK1101 decreased stem length and leaf area. Concentration-dependent inhibitions and dose response curves were observed. Although the effects of the inhibitors on dry weight varied according to the environmental conditions, the net assimilation rate was not influenced by the inhibitors. Accordingly, the observed decrease in dry weight caused by the inhibitors may result from decreased leaf area. Validation of the model based on observed data independent of the dataset showed good correlations between the observed and predicted values of dry weight and leaf area index.
authors.affiliation: National Agriculture and Food Research Organization, NARO Institute of Vegetable and Tea Science, Tsukuba 305-8666, Japan.
authors.forename: Tadahisa
authors.initials: T
authors.lastname: Higashide
authors.affiliation: 1] Kihara Institute for Biological Research, Yokohama City University, Yokohama 244-0813, Japan [2].
authors.forename: Megumi
authors.initials: M
authors.lastname: Narukawa
authors.affiliation: Kihara Institute for Biological Research, Yokohama City University, Yokohama 244-0813, Japan.
authors.forename: Yukihisa
authors.initials: Y
authors.lastname: Shimada
authors.affiliation: National Agriculture and Food Research Organization, NARO Western Region Agricultural Research Center, Zentsuji 765-0053, Japan.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Soeno
mesh_terms: D004777:Environment; D007210:Indoleacetic Acids / Q000037:antagonists & inhibitors* / Q000378:metabolism; D018551:Solanum lycopersicum / Q000187:drug effects* / Q000254:growth & development* / Q000378:metabolism; D010649:Phenylalanine / Q000031:analogs & derivatives / Q000494:pharmacology; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism; D036226:Seedlings / Q000187:drug effects* / Q000254:growth & development* / Q000378:metabolism
chemical_list: D007210:Indoleacetic Acids; C019145:alpha-aminooxy-beta-phenylpropionic acid; D010649:Phenylalanine