title: Enhanced repair of a critical-sized segmental bone defect in rabbit femur by surface microstructured porous titanium.
abstract: Repair of load-bearing bone defects remains a challenge in the field of orthopaedic surgery. In the current study, a surface microstructured porous titanium (STPT) successively treated with H2O2/TaCl5 solution and simulated body fluid was used to repair the critical-sized segmental bone defects in rabbit femur, and non-treated porous titanium (NTPT) and porous biphasic calcium phosphate ceramics (PBCP) were used as control, respectively. A 15 mm long implant was positioned in the femoral defect and stabilized by a plate and screws fixation. After implantation into the body for 1, 3 and 6 months, X-ray observation confirmed that porous titanium groups (NTPT and STPT) provided better mechanical support than PBCP group at the early stage. However, there was no obvious difference in the formed bony callus between PBCP and STPT groups in the later stage, and they both showed better shape of bony callus than NTPT group. Micro-CT and histomorphometric analysis for the samples of 6-month implantation demonstrated that more new bone formed in the inner pores of PBCP and STPT groups than that in NTPT group. Moreover, the biomechanical tests revealed that STPT group could bear larger compressive load than NTPT and PBCP groups, almost reaching the level of the normal rabbit femur. STPT exhibited the enhanced repairing effect on the critical-sized segmental bone defect in rabbit femur, meaning that it could be an ideal material for the repair of large bone defect in load-bearing site.
authors.affiliation: National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, 610064, China.
authors.forename: J
authors.initials: J
authors.lastname: Yang
authors.forename: H J
authors.initials: HJ
authors.lastname: Chen
authors.forename: X D
authors.initials: XD
authors.lastname: Zhu
authors.forename: S
authors.initials: S
authors.lastname: Vaidya
authors.forename: Z
authors.initials: Z
authors.lastname: Xiang
authors.forename: Y J
authors.initials: YJ
authors.lastname: Fan
authors.forename: X D
authors.initials: XD
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D001696:Biomechanical Phenomena; D018786:Bone Substitutes; D002130:Calcium Phosphates / Q000737:chemistry; D005269:Femur / Q000473:pathology*; D008297:Male; D008422:Materials Testing; D016062:Porosity; D019736:Prostheses and Implants; D011474:Prosthesis Design; D011817:Rabbits; D013314:Stress, Mechanical; D013977:Tibia / Q000473:pathology*; D014025:Titanium / Q000737:chemistry*; D014048:Tolonium Chloride / Q000737:chemistry; D016474:Weight-Bearing; D055114:X-Ray Microtomography
chemical_list: D001672:Biocompatible Materials; D018786:Bone Substitutes; D002130:Calcium Phosphates; D014048:Tolonium Chloride; C020243:calcium phosphate; D014025:Titanium