title: Anti-inflammatory and antioxidant effects of Aloe saponaria Haw in a model of UVB-induced paw sunburn in rats.
abstract: Ultraviolet B (UVB) irradiation mainly affects biological tissues by inducing an increase in reactive oxygen species (ROS) production which leads to deleterious outcomes for the skin, including pain and inflammation. As a protective strategy, many studies have focused on the use of natural products. The aim of this study was to investigate the effects of Aloe saponaria on nociceptive, inflammatory, and oxidative parameters in a model of UVB-induced sunburn in adult male Wistar rats. Sunburned animals were topically treated with vehicle (base cream), 1% silver sulfadiazine (positive control) or A. saponaria (10%) once a day for 6days. UVB-induced nociception (allodynia and hyperalgesia), inflammation (edema and leukocyte infiltration) and oxidative stress (increases in H2O2, protein carbonyl levels and lipid peroxidation and a decrease in non protein thiol content) were reduced by both A. saponaria and sulfadiazine topical treatment. Furthermore, A. saponaria or its constituents aloin and rutin reduced the oxidative stress induced by H2O2 in skin homogenates in vitro. Our results demonstrate that topical A. saponaria treatment displayed anti-nociceptive and anti-inflammatory effects in a UVB-induced sunburn model, and these effects seem to be related to its antioxidant components.
authors.affiliation: Programa de Pós Graduação em Ciências Biológicas, Bioquímica Toxicológica, Universidade Federal de Santa Maria (UFSM), 97109-900 Santa Maria, RS, Brazil; Departamento de Saúde, Curso de Biomedicina, Faculdade de Educação e Cultura de Vilhena, 76980-000 Vilhena, RO, Brazil.
authors.forename: Mariane Arnoldi
authors.initials: MA
authors.lastname: Silva
authors.affiliation: Programa de Pós Graduação em Ciências Biológicas, Bioquímica Toxicológica, Universidade Federal de Santa Maria (UFSM), 97109-900 Santa Maria, RS, Brazil; Programa de Pós-Graduação em Ciências da Saúde, Universidade do Extremo Sul Catarinense (UNESC), 88806-000, Criciúma, SC, Brazil.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Trevisan
authors.affiliation: Programa de Pós-graduação em Farmacologia, Universidade Federal de Santa Maria (UFSM), 97109-900 Santa Maria, RS, Brazil; Departamento de Saúde, Curso de Biomedicina, Faculdade de Educação e Cultura de Vilhena, 76980-000 Vilhena, RO, Brazil.
authors.forename: Carin
authors.initials: C
authors.lastname: Hoffmeister
authors.affiliation: Programa de Pós Graduação em Ciências Biológicas, Bioquímica Toxicológica, Universidade Federal de Santa Maria (UFSM), 97109-900 Santa Maria, RS, Brazil.
authors.forename: Mateus Fortes
authors.initials: MF
authors.lastname: Rossato
authors.affiliation: Programa de Pós Graduação em Ciências Farmacêuticas, Universidade Federal de Santa Maria (UFSM), 97109-900 Santa Maria, RS, Brazil.
authors.forename: Aline Augusti
authors.initials: AA
authors.lastname: Boligon
authors.affiliation: Departamento de Saúde, Curso de Farmácia, Universidade Federal de Sergipe (UFS), 49400-000 Lagarto, SE, Brazil.
authors.forename: Cristiani Isabel Banderò
authors.initials: CI
authors.lastname: Walker
authors.affiliation: Programa de Pós Graduação em Atenção Integral à Saúde, Universidade de Cruz Alta, 98020-290 Cruz Alta, RS, Brazil.
authors.forename: Jonatas Zeni
authors.initials: JZ
authors.lastname: Klafke
authors.affiliation: Programa de Pós Graduação em Ciências Biológicas, Bioquímica Toxicológica, Universidade Federal de Santa Maria (UFSM), 97109-900 Santa Maria, RS, Brazil.
authors.forename: Sara Marchesan
authors.initials: SM
authors.lastname: Oliveira
authors.affiliation: Programa de Pós Graduação em Ciências Biológicas, Bioquímica Toxicológica, Universidade Federal de Santa Maria (UFSM), 97109-900 Santa Maria, RS, Brazil.
authors.forename: Cássia Regina
authors.initials: CR
authors.lastname: Silva
authors.affiliation: Programa de Pós Graduação em Ciências Farmacêuticas, Universidade Federal de Santa Maria (UFSM), 97109-900 Santa Maria, RS, Brazil.
authors.forename: Margareth Linde
authors.initials: ML
authors.lastname: Athayde
authors.affiliation: Programa de Pós Graduação em Ciências Biológicas, Bioquímica Toxicológica, Universidade Federal de Santa Maria (UFSM), 97109-900 Santa Maria, RS, Brazil; Programa de Pós-graduação em Farmacologia, Universidade Federal de Santa Catarina (UFSC), 88049-900 Florianópolis, SC, Brazil. Electronic address: ferreiraj99@gmail.com.
authors.forename: Juliano
authors.initials: J
authors.lastname: Ferreira
mesh_terms: D000700:Analgesics / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D002851:Chromatography, High Pressure Liquid; D004195:Disease Models, Animal; D004642:Emodin / Q000031:analogs & derivatives / Q000032:analysis / Q000494:pharmacology / Q000627:therapeutic use; D007249:Inflammation / Q000188:drug therapy; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects / Q000528:radiation effects; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D029750:Saponaria / Q000737:chemistry* / Q000378:metabolism; D012837:Silver Sulfadiazine / Q000737:chemistry; D012867:Skin / Q000187:drug effects* / Q000528:radiation effects; D013471:Sunburn / Q000188:drug therapy; D013997:Time Factors; D014466:Ultraviolet Rays
chemical_list: D000700:Analgesics; D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D010936:Plant Extracts; D004642:Emodin; C006457:alloin; D012837:Silver Sulfadiazine
keywords: Aloin; Analgesic; Anti-inflammatory; Antioxidant; Rutin; Silver sulfadiazine