title: Simultaneous determination of matrine and berberine in fruits, vegetables, and soil using ultra-performance liquid chromatography/tandem mass spectrometry.
abstract: An ultra-performance LC/tandem MS (UPLC/MS/MS) method for the simultaneous quantification and identification of matrine and berberine, alkaloids widely used in plant fungicides, has been developed and validated. Methanol or 1% ammonia-acetonitrile were selected as extraction solvents, and primary secondary amine sorbent was chosen for cleanup to achieve satisfactory recoveries in accordance with European Union guidelines. The chromatographic separation was carried out on a hydrophilic interaction LC column with a UPLC/MS/MS-based method to improve the retentions and shapes of the peaks. Method validation was performed for linearity, repeatability, interday precision, and sensitivity. Recoveries and RSDs were acceptable (73.1-109.3% recovery, RSD < or = 15.8%). The LODs varied from 0.34 to 1.07 microg/kg for matrine and 0.09 to 0.18 microg/kg for berberine, while the LOQs ranged from 1.12 to 3.58 microg/kg for matrine and 0.31 to 0.60 microg/kg for berberine. This method was successfully and efficiently applied for the analysis of matrine and berberine in real samples.
authors.forename: Xingang
authors.initials: X
authors.lastname: Liu
authors.forename: Yingying
authors.initials: Y
authors.lastname: Tian
authors.forename: Fengshou
authors.initials: F
authors.lastname: Dong
authors.forename: Jun
authors.initials: J
authors.lastname: Xu
authors.forename: Yuanbo
authors.initials: Y
authors.lastname: Li
authors.forename: Xuyang
authors.initials: X
authors.lastname: Liang
authors.forename: Yunhao
authors.initials: Y
authors.lastname: Wang
authors.forename: Yongquan
authors.initials: Y
authors.lastname: Zheng
mesh_terms: D000470:Alkaloids / Q000737:chemistry*; D001599:Berberine / Q000737:chemistry*; D002853:Chromatography, Liquid; D005504:Food Analysis / Q000379:methods; D005638:Fruit / Q000737:chemistry*; D011807:Quinolizines / Q000737:chemistry*; D015203:Reproducibility of Results; D012987:Soil / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods; D014675:Vegetables / Q000737:chemistry*; D000093842:Matrines
chemical_list: D000470:Alkaloids; D011807:Quinolizines; D012987:Soil; D001599:Berberine; D000093842:Matrines