title: Comparison of Enterococcus quantitative polymerase chain reaction analysis results from Midwest U.S. river samples using EPA Method 1611 and Method 1609 PCR reagents.
abstract: Enterococci target sequence density estimates from analyses of diluted river water DNA extracts by EPA Methods 1611 and 1609 and estimates with lower detection limits from undiluted DNA extracts by Method 1609 were indistinguishable. These methods should be equally suitable for comparison with U.S. EPA 2012 Recreational Water Quality Criteria values.
authors.affiliation: U.S. Environmental Protection Agency, Office of Research and Development, National Risk Management Research Laboratory, 26 West Martin Luther King Drive, Cincinnati, OH 45268, USA.
authors.forename: Mano
authors.initials: M
authors.lastname: Sivaganesan
authors.forename: Mano
authors.initials: M
authors.lastname: Sivaganensan
authors.affiliation: U.S. Environmental Protection Agency, Office of Research and Development, National Exposure Research Laboratory, 26 West Martin Luther King Drive, Cincinnati, OH 45268, USA.
authors.forename: Shawn
authors.initials: S
authors.lastname: Siefring
authors.affiliation: U.S. Environmental Protection Agency, Office of Research and Development, National Exposure Research Laboratory, 26 West Martin Luther King Drive, Cincinnati, OH 45268, USA.
authors.forename: Manju
authors.initials: M
authors.lastname: Varma
authors.affiliation: U.S. Environmental Protection Agency, Office of Research and Development, National Exposure Research Laboratory, 26 West Martin Luther King Drive, Cincinnati, OH 45268, USA. Electronic address: haugland.rich@epa.gov.
authors.forename: Richard A
authors.initials: RA
authors.lastname: Haugland
mesh_terms: D016983:Enterococcus / Q000145:classification / Q000235:genetics*; D007202:Indicators and Reagents / Q000737:chemistry; D057230:Limit of Detection; D015146:Midwestern United States; D058889:Molecular Typing / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D045483:Rivers / Q000382:microbiology*
chemical_list: D007202:Indicators and Reagents
keywords: EPA Method 1609; EPA Method 1611; Enterococcus; Recreational water quality; qPCR