title: Effect of fluorosis on liver cells of VC deficient and wild type mice.
abstract: For decades, mouse and other rodents have been used for the study of oxidative or related studies such as the effect of fluoride. It is known that rodents normally synthesize their own vitamin C (VC) due to the presence of a key enzyme in ascorbic acid synthesis, l-gulono-lactone-γ-oxidase (Gulo), while humans do not have the capacity of VC synthesis due to the deletion of most parts of the GULO gene. The spontaneous fracture (sfx) mouse recently emerged as a model for study of VC deficiency. We investigated the effect of fluoride on liver cells from wild type Balb/c and sfx mice. We found that activities of SOD, GPx, and CAT were reduced in both wild type and sfx mice; however, the amount of reduction in the sfx cells is more than that in Balb/c cells. In addition, while both cells increased MDA, the increase in the sfx cells is greater than that in Balb/c cells. Gene networks of Sod, Gpx, and Cat in the liver of humans and mice are also different. Our study suggests that reaction to fluoride in vitamin C deficient mice might be different from that of wild type mice.
authors.affiliation: Center for Endemic Disease Control, Centers for Disease Control and Prevention, Institute of Endemic Fluorosis Disease, Harbin Medical University, Harbin, Heilongjiang 150081, China ; Departments of Orthopaedic Surgery and BME, Campbell Clinic, and Pathology, University of Tennessee Health Science Center (UTHSC), 956 Court Avenue, Memphis, TN 38163, USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Wei
authors.affiliation: Departments of Orthopaedic Surgery and BME, Campbell Clinic, and Pathology, University of Tennessee Health Science Center (UTHSC), 956 Court Avenue, Memphis, TN 38163, USA ; Mudanjiang Medical College, Mudanjiang, Heilongjiang 157001, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Jiao
authors.affiliation: Departments of Orthopaedic Surgery and BME, Campbell Clinic, and Pathology, University of Tennessee Health Science Center (UTHSC), 956 Court Avenue, Memphis, TN 38163, USA.
authors.forename: Yonghui
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Division of Connective Tissues Diseases, Department of Medicine, University of Tennessee Health Science Center, Memphis, TN 38163, USA ; Department of Veterans Affairs Medical Center, Memphis, TN 38104, USA.
authors.forename: John M
authors.initials: JM
authors.lastname: Stuart
authors.affiliation: Department of Nephrology, Second Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang 150001, China.
authors.forename: Xiudian
authors.initials: X
authors.lastname: Li
authors.affiliation: Departments of Orthopaedic Surgery and BME, Campbell Clinic, and Pathology, University of Tennessee Health Science Center (UTHSC), 956 Court Avenue, Memphis, TN 38163, USA ; Mudanjiang Medical College, Mudanjiang, Heilongjiang 157001, China.
authors.forename: Fusheng
authors.initials: F
authors.lastname: Zhao
authors.affiliation: Departments of Orthopaedic Surgery and BME, Campbell Clinic, and Pathology, University of Tennessee Health Science Center (UTHSC), 956 Court Avenue, Memphis, TN 38163, USA.
authors.forename: Lishi
authors.initials: L
authors.lastname: Wang
authors.affiliation: Center for Endemic Disease Control, Centers for Disease Control and Prevention, Institute of Endemic Fluorosis Disease, Harbin Medical University, Harbin, Heilongjiang 150081, China.
authors.forename: Dianjun
authors.initials: D
authors.lastname: Sun
authors.affiliation: Departments of Orthopaedic Surgery and BME, Campbell Clinic, and Pathology, University of Tennessee Health Science Center (UTHSC), 956 Court Avenue, Memphis, TN 38163, USA.
authors.forename: Weikuan
authors.identifier: 0000-0003-1112-8088
authors.initials: W
authors.lastname: Gu
mesh_terms: D000818:Animals; D001206:Ascorbic Acid Deficiency / Q000378:metabolism*; D002478:Cells, Cultured; D005459:Fluorides / Q000494:pharmacology*; D009050:Fluorosis, Dental / Q000378:metabolism*; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D018384:Oxidative Stress / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D017382:Reactive Oxygen Species; D005459:Fluorides