title: Enhancing mucosal immunity in mice by recombinant adenovirus expressing major epitopes of porcine circovirus-2 capsid protein delivered with cytosine-phosphate-guanosine oligodeoxynucleotides.
abstract: A recombinant replication-defective adenovirus expressing the major epitopes of porcine circovirus-2 (PCV-2) capsid protein (rAd/Cap/518) was previously constructed and shown to induce mucosal immunity in mice following intranasal delivery. In the present study, immune responses induced by intranasal immunization with a combination of rAd/Cap/518 and cytosine-phosphate-guanosine oligodeoxynucleotides (CpG ODN) were evaluated in mice. The levels of PCV-2-specific IgG in serum and IgA in saliva, lung, and intestinal fluids were significantly higher in the group immunized with rAd/Cap/518 and CpG ODN than animals immunized with rAd/Cap/518 alone. The frequencies of IL-2-secreting CD4⁺ T cells and IFN-γ-producing CD8⁺ T cells were significantly higher in the combined immunization group than mice immunized with rAd/Cap/518 alone. The frequencies of CD3⁺, CD3⁺CD4⁺CD8⁻, and CD3⁺CD4⁻CD8⁺ T cells in the combined immunization group were similar to that treated with CpG ODN alone, but significantly higher than mice that did not receive CpG ODN. PCV-2 load after challenge in the combined immunization group was significantly lower than that in the phosphate-buffered saline placebo group and approximately 7-fold lower in the group treated with CpG ODN alone. These results indicate that rAd/Cap/518 combined with CpG ODN can enhance systemic and local mucosal immunity in mice, and represent a promising synergetic mucosal vaccine against PCV-2.
authors.affiliation: Animal Infectious Disease Lab, College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China.
authors.forename: Hong-Tao
authors.initials: HT
authors.lastname: Chang
authors.forename: Xiu-Yuan
authors.initials: XY
authors.lastname: He
authors.forename: Yu-Feng
authors.initials: YF
authors.lastname: Liu
authors.forename: Lu
authors.initials: L
authors.lastname: Chen
authors.forename: Quan-Hai
authors.initials: QH
authors.lastname: Guo
authors.forename: Qiu-Ying
authors.initials: QY
authors.lastname: Yu
authors.forename: Jun
authors.initials: J
authors.lastname: Zhao
authors.forename: Xin-Wei
authors.initials: XW
authors.lastname: Wang
authors.forename: Xia
authors.initials: X
authors.lastname: Yang
authors.forename: Chuan-Qing
authors.initials: CQ
authors.lastname: Wang
mesh_terms: D000256:Adenoviridae / Q000235:genetics / Q000276:immunology; D000281:Administration, Intranasal; D000818:Animals; D036022:Capsid Proteins / Q000235:genetics* / Q000276:immunology; D018173:Circoviridae Infections / Q000276:immunology*; D017925:Circovirus / Q000235:genetics* / Q000276:immunology; D000939:Epitopes / Q000235:genetics / Q000276:immunology; D005260:Female; D018928:Immunity, Mucosal / Q000276:immunology; D007070:Immunoglobulin A / Q000097:blood / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009838:Oligodeoxyribonucleotides / Q000235:genetics; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage / Q000235:genetics* / Q000276:immunology
chemical_list: D036022:Capsid Proteins; D000939:Epitopes; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D009838:Oligodeoxyribonucleotides; D014614:Vaccines, Synthetic; D014765:Viral Vaccines
keywords: cytosine-phosphate-guanosine oligodeoxynucleotides; mucosal immunity; porcine circovirus-2; rAd/Cap/518