title: Sunitinib Improves Some Clinical Aspects and Reverts DMBA-Induced Hyperplasic Lesions in Hamster Buccal Pouch.
abstract: Oral squamous cell carcinoma (OSCC) is a public health problem. The hamster buccal pouch model is ideal for analyzing the development of OSCC. This research analysed the effects of sunitinib (tyrosine kinase inhibitor) in precancerous lesions induced by 7,12-dimethylbenz(a)anthracene (DMBA) in this model. Thirty-four male hamsters, divided into six groups: control-C (n = 7), acetone-A (n = 12), carbamide peroxide-CP (n = 5 ), acetone and CP-A+CP (n = 8), 1% DMBA in acetone and CP-DA+CP (n = 6), and 1% DMBA in acetone and CP and 4-week treatment with sunitinib-DA+CP+S (n = 7). The aspects evaluated were anatomopathological features (peribuccal area, paws, nose, and fur), histological sections of the hamster buccal pouches (qualitatively analyzed), epithelium thickness, and the rete ridge density (estimated). Sunitinib was unable to attenuate the decrease in weight gain induced by DMBA; no increase in volume was detected in the pouch and/or ulceration, observed in 43% of the animals in the DA+CP group. DA+CP groups presented a significant increase in rete ridge density compared to the control groups (P < 0.01) which was reverted by sunitinib in the DA+CP+S group. Sunitinib seems to have important benefits in early stage carcinogenesis and may be useful in chemoprevention.
authors.affiliation: Laboratório de Biologia Celular e Tecidual, Faculdade de Biociências, PUCRS, Avenida Ipiranga 6681, Prédio 12, Sala 104, 90619-900 Porto Alegre, RS, Brazil.
authors.forename: Fernanda Lopes
authors.initials: FL
authors.lastname: de Souza
authors.affiliation: Laboratório de Biologia Celular e Tecidual, Faculdade de Biociências, PUCRS, Avenida Ipiranga 6681, Prédio 12, Sala 104, 90619-900 Porto Alegre, RS, Brazil.
authors.forename: Mariana
authors.initials: M
authors.lastname: Oliveira
authors.affiliation: Laboratório de Biologia Celular e Tecidual, Faculdade de Biociências, PUCRS, Avenida Ipiranga 6681, Prédio 12, Sala 104, 90619-900 Porto Alegre, RS, Brazil.
authors.forename: Marianne Brochado
authors.initials: MB
authors.lastname: Nunes
authors.affiliation: Laboratório de Biologia Celular e Tecidual, Faculdade de Biociências, PUCRS, Avenida Ipiranga 6681, Prédio 12, Sala 104, 90619-900 Porto Alegre, RS, Brazil.
authors.forename: Lucas Horstmann
authors.initials: LH
authors.lastname: Serafim
authors.affiliation: Laboratório de Biologia Celular e Tecidual, Faculdade de Biociências, PUCRS, Avenida Ipiranga 6681, Prédio 12, Sala 104, 90619-900 Porto Alegre, RS, Brazil.
authors.forename: Alan Arrieira
authors.initials: AA
authors.lastname: Azambuja
authors.affiliation: Laboratório de Biologia Celular e Tecidual, Faculdade de Biociências, PUCRS, Avenida Ipiranga 6681, Prédio 12, Sala 104, 90619-900 Porto Alegre, RS, Brazil.
authors.forename: Luisa Maria G de M
authors.initials: LM
authors.lastname: Braga
authors.affiliation: Laboratório de Biologia Celular e Tecidual, Faculdade de Biociências, PUCRS, Avenida Ipiranga 6681, Prédio 12, Sala 104, 90619-900 Porto Alegre, RS, Brazil.
authors.forename: Lisiani
authors.initials: L
authors.lastname: Saur
authors.affiliation: Laboratório de Biologia Celular e Tecidual, Faculdade de Biociências, PUCRS, Avenida Ipiranga 6681, Prédio 12, Sala 104, 90619-900 Porto Alegre, RS, Brazil.
authors.forename: Maria Antonieta Lopes
authors.initials: MA
authors.lastname: de Souza
authors.affiliation: Laboratório de Biologia Celular e Tecidual, Faculdade de Biociências, PUCRS, Avenida Ipiranga 6681, Prédio 12, Sala 104, 90619-900 Porto Alegre, RS, Brazil.
authors.forename: Léder Leal
authors.initials: LL
authors.lastname: Xavier