title: An in vitro comparison of antimicrobial effcacy of three root canal irrigants-BioPure MTAD, 2% chlorhexidine gluconate and 5.25% sodium hypochlorite as a final rinse against E. faecalis.
abstract: OBJECTIVE
  This study was conducted to evaluate the antimicrobial activity of 5.25% sodium hypochlorite (NaOCl), 2% chlorhexidine (CHX) and BioPure MTAD when used as a fnal rinse against Enterococcus faecalis.
  
  
  METHODS
  Sixty single-rooted premolars were biomechanically prepared, inoculated with E. faecalis and divided into various groups. These were then irrigated with the test irrigants and tested microbiologically for growth of E. faecalis immediately after irrigation and after 48 hours.
  
  
  RESULTS
  Statistical analysis showed that there was a signifcant difference between the antibacterial activities of BioPure MTAD, 2% CHX and 5.25% NaOCl at 5 minutes; however, the antibacterial activities of the three irrigants were comparable after 2 days of irrigation.
  
  
  CONCLUSIONS
  The present study concludes that BioPure MTAD is as effective against E. faecalis as 5.25% NaOCl and more effective than 2% CHX. CLINICAL SIGNIFCANCE: E. faecalis is one of the most resistant intracanal species and a possible cause of root canal failure. Many authors have stressed the importance of using antimicrobial irrigants during chemomechanical preparation to ensure complete disinfection. Therefore, various irrigating solutions have been used during and immediately after root canal preparation to remove debris and necrotic pulp tissue and to eliminate microorganisms that cannot be reached by mechanical instrumentation.
authors.affiliation: Professor and Head, Department of Conservative Dentistry and Endodontics, Triveni Institute of Dental Sciences and Research Centre Bilaspur, Chhattisgarh, India.
authors.forename: Vinod
authors.initials: V
authors.lastname: Agrawal
authors.affiliation: Professor and Head, Department of Conservative Dentistry and Endodontics, Sree Sai Dental College and Research Institute Srikakulam, Andhra Pradesh, India.
authors.forename: Ms Rama
authors.initials: MR
authors.lastname: Rao
authors.affiliation: Consultant Endodontist, Mumbai, Maharashtra, India, e-mail: kp.dhingra@gmail.com.
authors.forename: Kanupriya
authors.initials: K
authors.lastname: Dhingra
authors.affiliation: Professor and Head, Department of Conservative Dentistry and Endodontics, Best Dental Science College, Melur Road, Ultra Nagar Madurai, Tamil Nadu, India.
authors.forename: V Rajesh
authors.initials: VR
authors.lastname: Gopal
authors.affiliation: Reader, Department of Prosthodontics, Institute of Dental Sciences Khandagiri, Bhubaneswar, Odisha, India.
authors.forename: Abhijita
authors.initials: A
authors.lastname: Mohapatra
authors.affiliation: Senior Lecturer, Department of Prosthodontics, Institute of Dental Sciences, Khandagiri, Bhubaneswar, Odisha, India.
authors.forename: Abhilash
authors.initials: A
authors.lastname: Mohapatra
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D058491:Bacterial Load / Q000187:drug effects; D001431:Bacteriological Techniques; D002710:Chlorhexidine / Q000031:analogs & derivatives / Q000494:pharmacology; D019343:Citric Acid / Q000494:pharmacology; D019551:Dental Disinfectants / Q000494:pharmacology; D003786:Dental Pulp Cavity / Q000382:microbiology; D004318:Doxycycline / Q000494:pharmacology; D013293:Enterococcus faecalis / Q000187:drug effects* / Q000254:growth & development; D006801:Humans; D008422:Materials Testing; D011136:Polysorbates / Q000494:pharmacology; D012388:Root Canal Irrigants / Q000494:pharmacology*; D012973:Sodium Hypochlorite / Q000494:pharmacology; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D019551:Dental Disinfectants; C475043:MTAD (intracanal irrigant); D011136:Polysorbates; D012388:Root Canal Irrigants; D019343:Citric Acid; D012973:Sodium Hypochlorite; C010882:chlorhexidine gluconate; D004318:Doxycycline; D002710:Chlorhexidine