title: Generation of IL-8 and IL-9 producing CD4⁺ T cells is affected by Th17 polarizing conditions and AHR ligands.
abstract: The T helper cell subsets Th1, Th2, Th17, and Treg play an important role in immune cell homeostasis, in host defense, and in immunological disorders. Recently, much attention has been paid to Th17 cells which seem to play an important role in the early phase of the adoptive immune response and autoimmune disease. When generating Th17 cells under in vitro conditions the amount of IL-17A producing cells hardly exceeds 20% while the nature of the remaining T cells is poorly characterized. As engagement of the aryl hydrocarbon receptor (AHR) has also been postulated to modulate the differentiation of T helper cells into Th17 cells with regard to the IL-17A expression we ask how far do Th17 polarizing conditions in combination with ligand induced AHR activation have an effect on the production of other T helper cell cytokines. We found that a high proportion of T helper cells cultured under Th17 polarizing conditions are IL-8 and IL-9 single producing cells and that AHR activation results in an upregulation of IL-8 and a downregulation of IL-9 production. Thus, we have identified IL-8 and IL-9 producing T helper cells which are subject to regulation by the engagement of the AHR.
authors.affiliation: UFZ-Helmholtz Centre for Environmental Research Leipzig, Department of Environmental Immunology, Permoserstrasse, 04318 Leipzig, Germany.
authors.forename: Michaela
authors.initials: M
authors.lastname: Gasch
authors.affiliation: UFZ-Helmholtz Centre for Environmental Research Leipzig, Department of Environmental Immunology, Permoserstrasse, 04318 Leipzig, Germany.
authors.forename: Tina
authors.initials: T
authors.lastname: Goroll
authors.affiliation: UFZ-Helmholtz Centre for Environmental Research Leipzig, Department of Environmental Immunology, Permoserstrasse, 04318 Leipzig, Germany.
authors.forename: Mario
authors.initials: M
authors.lastname: Bauer
authors.affiliation: UFZ-Helmholtz Centre for Environmental Research Leipzig, Department of Environmental Immunology, Permoserstrasse, 04318 Leipzig, Germany.
authors.forename: Denise
authors.initials: D
authors.lastname: Hinz
authors.affiliation: Junior Research Group Immune Pathogenesis of New Allergies, Leipzig Research Centre for Civilization Diseases, University of Leipzig, Johannisallee, 04103 Leipzig, Germany.
authors.forename: Nicole
authors.initials: N
authors.lastname: Schütze
authors.affiliation: UFZ-Helmholtz Centre for Environmental Research Leipzig, Department of Environmental Immunology, Permoserstrasse, 04318 Leipzig, Germany.
authors.forename: Tobias
authors.initials: T
authors.lastname: Polte
authors.affiliation: Institute for Laboratory Medicine and Pathobiochemistry, Philipps University of Marburg, Hans-Meerwein Strasse, 35043 Marburg, Germany.
authors.forename: Dörthe
authors.initials: D
authors.lastname: Kesper
authors.affiliation: Universitätsklinikum, Klinik für Dermatologie, Venerologie und Allergologie, Philipp-Rosenthal Strasse, 04103 Leipzig, Germany.
authors.forename: Jan C
authors.initials: JC
authors.lastname: Simon
authors.affiliation: Young Investigators Group Bioinformatics and Transcriptomics, Department of Proteomics, UFZ-Helmholtz Centre for Environmental Research, Permoserstrasse, 04318 Leipzig, Germany.
authors.forename: Jörg
authors.initials: J
authors.lastname: Hackermüller
authors.affiliation: UFZ-Helmholtz Centre for Environmental Research Leipzig, Department of Environmental Immunology, Permoserstrasse, 04318 Leipzig, Germany.
authors.forename: Irina
authors.initials: I
authors.lastname: Lehmann
authors.affiliation: UFZ-Helmholtz Centre for Environmental Research Leipzig, Department of Environmental Immunology, Permoserstrasse, 04318 Leipzig, Germany.
authors.forename: Gunda
authors.initials: G
authors.lastname: Herberth
mesh_terms: D001327:Autoimmune Diseases / Q000378:metabolism; D051792:Basic Helix-Loop-Helix Proteins / Q000378:metabolism*; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism*; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D016207:Cytokines / Q000378:metabolism; D006706:Homeostasis; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism*; D016906:Interleukin-9 / Q000378:metabolism*; D008024:Ligands; D018336:Receptors, Aryl Hydrocarbon / Q000378:metabolism*; D058504:Th17 Cells / Q000166:cytology*
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D016207:Cytokines; D016209:Interleukin-8; D016906:Interleukin-9; D008024:Ligands; D018336:Receptors, Aryl Hydrocarbon; C111235:AHR protein, human; C508606:CXCL8 protein, human; C508607:IL9 protein, human