title: Molecular characterization and tissue expression of peptide YY in Schizothorax prenanti: effects of periprandial changes and fasting on expression in the hypothalamus.
abstract: Peptide YY (PYY) is a potent anorectic neuropeptide implicated in feeding regulation in mammals. However, the involvement of PYY in the feeding behavior of teleosts has not been well understood. In this study, we employed molecular, real-time quantitative PCR and physiological studies to characterize the structure, distribution, and appetite regulatory effects of PYY in Schizothorax prenanti (S. prenanti). A very high conservation in PYY sequences was found in teleosts. PYY is widely expressed, with the highest levels of expression in telencephalon, medulla oblongata, pituitary and hypothalamus of S. prenanti. The PYY mRNA expression in the hypothalamus was highly elevated after a meal, suggesting a satiety signal role for PYY in S. prenanti. In addition, PYY gene expression in the hypothalamus was decreased after fasting and increased sharply after refeeding, which suggested that PYY might be involved in the central regulation of appetite in S. prenanti. Overall, our result provides basis for further investigation into the regulation of feeding in S. prenanti.
authors.affiliation: Department of Aquaculture, College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, China.
authors.forename: Dengyue
authors.initials: D
authors.lastname: Yuan
authors.affiliation: Department of Aquaculture, College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, China.
authors.forename: Chaowei
authors.initials: C
authors.lastname: Zhou
authors.affiliation: Department of Aquaculture, College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.affiliation: Department of Aquaculture, College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, China.
authors.forename: Fangjun
authors.initials: F
authors.lastname: Lin
authors.affiliation: Department of Aquaculture, College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, China.
authors.forename: Hu
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Aquaculture, College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Wu
authors.affiliation: Department of Aquaculture, College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, China.
authors.forename: Rongbin
authors.initials: R
authors.lastname: Wei
authors.affiliation: Department of Aquaculture, College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, China.
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Xin
authors.affiliation: Department of Aquaculture, College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, China.
authors.forename: Ju
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Aquaculture, College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, China.
authors.forename: Yundi
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Department of Aquaculture, College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, China.
authors.forename: Defang
authors.initials: D
authors.lastname: Chen
authors.affiliation: Department of Aquaculture, College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, China.
authors.forename: Shiyong
authors.initials: S
authors.lastname: Yang
authors.affiliation: Department of Aquaculture, College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Aquaculture, College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, China.
authors.forename: Yundan
authors.initials: Y
authors.lastname: Pu
authors.affiliation: Department of Aquaculture, College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, China. Electronic address: lizhiqiong454@163.com.
authors.forename: Zhiqiong
authors.initials: Z
authors.lastname: Li
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D003530:Cyprinidae / Q000378:metabolism*; D005215:Fasting; D020869:Gene Expression Profiling; D007031:Hypothalamus / Q000378:metabolism*; D019894:Peptide YY / Q000235:genetics* / Q000378:metabolism*; D019518:Postprandial Period; D011487:Protein Conformation; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D019894:Peptide YY
keywords: Fasting; Molecular characterization; PYY; Postprandial; Schizothorax prenanti