title: Heat shock protein 70 is involved in malignant behaviors and chemosensitivities to cisplatin in cervical squamous cell carcinoma cells.
abstract: OBJECTIVE
  Heat shock protein 70 (HSP70) was previously found to be highly expressed in cervical squamous cell carcinoma (SCC) tissues compared with normal tissues by proteomic analyses. The present study investigated the roles of HSP70 in malignant behaviors and chemosensitivity to cisplatin in cervical SCC cells in vitro.
  
  
  METHODS
  Effects of HSP70 knockdown on activities of cell migration, cell invasion and cell proliferation, cell cycle status, and apoptosis were examined using siRNA in two human cervical cancer cells (SiHa and SKG II). Furthermore, the effect of HSP70 knockdown on the apoptotic effect by cisplatin was examined.
  
  
  RESULTS
  HSP70 knockdown significantly suppressed activities of cell migration, cell invasion and cell proliferation. The cell cycle was arrested at the S- and G2/M-phases with the increase in apoptosis by HSP70 knockdown. Although cisplatin had no apoptotic effect in the control cell, it showed a dose-dependent apoptotic effect in the HSP70 knockdown cells in SiHa. In SKG II, 5 μg/mL of cisplatin induced apoptosis, and the apoptotic effect by cisplatin was enhanced by HSP70 knockdown.
  
  
  CONCLUSIONS
  It was suggested that HSP70 played functional roles in the progression of uterine cervical SCC, and HSP70 knockdown enhanced chemosensitivities to cisplatin in cervical SCC cells.
authors.affiliation: Department of Obstetrics and Gynecology, Yamaguchi University Graduate School of Medicine, Ube, Japan.
authors.forename: Keiko
authors.initials: K
authors.lastname: Yoshidomi
authors.forename: Akihiro
authors.initials: A
authors.lastname: Murakami
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Yakabe
authors.forename: Kotaro
authors.initials: K
authors.lastname: Sueoka
authors.forename: Shugo
authors.initials: S
authors.lastname: Nawata
authors.forename: Norihiro
authors.initials: N
authors.lastname: Sugino
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy / Q000473:pathology*; D049109:Cell Proliferation / Q000187:drug effects; D002945:Cisplatin / Q000494:pharmacology*; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000502:physiology*; D006801:Humans; D014407:Tumor Cells, Cultured; D002583:Uterine Cervical Neoplasms / Q000188:drug therapy / Q000473:pathology*
chemical_list: D000970:Antineoplastic Agents; D018840:HSP70 Heat-Shock Proteins; D002945:Cisplatin
keywords: apoptosis; cervical squamous cell carcinoma; chemosensitivity; heat shock protein 70; siRNA