title: Streptomyces alboflavus RPS and its novel and high algicidal activity against harmful algal bloom species Phaeocystis globosa.
abstract: Phaeocystis globosa blooms have frequently occurred along coastal waters and exerted serious impacts on ecological environments by releasing toxic hemolytic substances, forming nuisance foam, and causing oxygen depletion. An actinomycete strain RPS with high algicidal activity against P. globosa was isolated and identified as Streptomyces alboflavus, based on morphology, physiological and biochemical characteristics, and 16S rDNA sequence analysis. RPS lysed 95% of P. globosa within 48 h by releasing an extracellular active substance into the growth medium. The activity of RPS supernatant was sensitive to temperature at and above 50 °C and insensitive to pH from 3 to 11. The molecular weight of the active substance was between 100 Da and 1000 Da, and approximately 90% of it was extracted by ethyl acetate. It was presumed that the active component efficiently inhibited the movement of P. globosa, caused the flagella to fall off the algae, and finally lysed the algal cells. RPS showed a wide target range against harmful algae. S. alboflavus RPS with high algicidal activity and such novel features of temperature and pH sensitivity, low molecular weight, algicidal process, and target range possesses great potential in the biological control of P. globosa blooms.
authors.affiliation: State Key Laboratory of Marine Environmental Science and Key Laboratory of the Ministry of Education for Coast and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen, Fujian, China.
authors.forename: Bangzhou
authors.initials: B
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Marine Environmental Science and Key Laboratory of the Ministry of Education for Coast and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen, Fujian, China.
authors.forename: Guanjing
authors.initials: G
authors.lastname: Cai
authors.affiliation: State Key Laboratory of Marine Environmental Science and Key Laboratory of the Ministry of Education for Coast and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen, Fujian, China.
authors.forename: Haitao
authors.initials: H
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Marine Environmental Science and Key Laboratory of the Ministry of Education for Coast and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen, Fujian, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Li
authors.affiliation: State Key Laboratory of Marine Environmental Science and Key Laboratory of the Ministry of Education for Coast and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen, Fujian, China.
authors.forename: Xujun
authors.initials: X
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Marine Environmental Science and Key Laboratory of the Ministry of Education for Coast and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen, Fujian, China.
authors.forename: Xinli
authors.initials: X
authors.lastname: An
authors.affiliation: State Key Laboratory of Marine Environmental Science and Key Laboratory of the Ministry of Education for Coast and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen, Fujian, China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Zheng
authors.affiliation: State Key Laboratory of Marine Environmental Science and Key Laboratory of the Ministry of Education for Coast and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen, Fujian, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Tian
authors.affiliation: State Key Laboratory of Marine Environmental Science and Key Laboratory of the Ministry of Education for Coast and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen, Fujian, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zheng
authors.affiliation: State Key Laboratory of Marine Environmental Science and Key Laboratory of the Ministry of Education for Coast and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen, Fujian, China.
authors.forename: Tianling
authors.initials: T
authors.lastname: Zheng
mesh_terms: D039903:Actinobacteria; D000898:Antibiosis; D058087:Haptophyta; D057097:Harmful Algal Bloom; D006863:Hydrogen-Ion Concentration; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013302:Streptomyces / Q000145:classification / Q000235:genetics; D013696:Temperature
chemical_list: D012336:RNA, Ribosomal, 16S