title: Metallothionein mRNA induction is correlated with the decrease of DNA strand breaks in cadmium exposed zebra mussels.
abstract: We have previously shown that cadmium (Cd) and benzo[a]pyrene (BaP) induced early DNA damages in zebra mussels, and that the level of DNA strand breaks (SB) returned to a basal level after 3 days of exposure to Cd. The aim of the present study was to go further in the mechanisms of Cd and BaP detoxification. For that purpose, expression of genes encoding for metallothionein (MT), aryl hydrocarbon receptor (AHR), P-gp, catalase, glutathione S-transferase and heat shock protein 70 (HSP70) proteins have been measured using RT-qPCR. Data reported here show that Cd is a strong inducer of MT and HSP70 genes, and that BaP is a strong inducer of P-gp and AHR genes. Exposure to Cd and BaP resulted in moderate changes in antioxidant enzymes mRNA. Since the increase of MT mRNA occurred when the DNA SB level returned to its basal level, we can suggest that MT is implicated in cadmium detoxification.
authors.affiliation: IRSTEA, Unité de Recherches Hydrosystèmes et Bioprocédés, 1 rue Pierre-Gilles de Gennes, CS 10030, 92761 Antony Cedex, France. Electronic address: francoise.vincent@ifremer.fr.
authors.forename: Françoise
authors.initials: F
authors.lastname: Vincent-Hubert
authors.affiliation: IRSTEA, Unité de Recherches Hydrosystèmes et Bioprocédés, 1 rue Pierre-Gilles de Gennes, CS 10030, 92761 Antony Cedex, France. Electronic address: amelie.chatel@uco.fr.
authors.forename: Amélie
authors.initials: A
authors.lastname: Châtel
authors.affiliation: IRSTEA, Unité de Recherches Hydrosystèmes et Bioprocédés, 1 rue Pierre-Gilles de Gennes, CS 10030, 92761 Antony Cedex, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Gourlay-Francé
mesh_terms: D000818:Animals; D001564:Benzo(a)pyrene / Q000633:toxicity; D002104:Cadmium / Q000493:pharmacokinetics / Q000633:toxicity*; D004249:DNA Damage; D018397:Equidae; D005786:Gene Expression Regulation / Q000187:drug effects*; D008668:Metallothionein / Q000096:biosynthesis*; D009124:Muscle Proteins / Q000096:biosynthesis*; D018482:Muscle, Skeletal / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000096:biosynthesis*
chemical_list: D009124:Muscle Proteins; D012333:RNA, Messenger; D002104:Cadmium; D001564:Benzo(a)pyrene; D008668:Metallothionein
keywords: Antioxidant enzymes; Aryl hydrocarbon receptor; Benzo[a]pyrene; Cadmium; Metallothionein; RT-qPCR