title: Chitinase 3 like 1 is associated with tumor angiogenesis in cervical cancer.
abstract: Elevated serum levels of a secreted glycoprotein chitinase 3 like 1 (CHI3L1) are associated with poor prognosis and short survival time of patients with cervical cancer (CxCa). Our previous microarray data showed the increased expression of CHI3L1 in invasive CxCa compared to normal tissue, implicating a potential role of CHI3L1 in CxCa. To establish the pathological role of CHI3L1 in the development of CxCa, this study focused on its expression in CxCa and angiogenic impacts in tumor vessel formation. CHI3L1 activated angiogenesis by promoting endothelial cell migration and tube formation in vitro but failed to protect CxCa cell lines, CaSki and HeLa against apoptosis induced by γ-irradiation. In addition, the capability of CHI3L1 to induce proliferation and migration of CaSki and HeLa cells was cell type specific. In an analysis of 103 specimens from CxCa patients, increased expression levels of CHI3L1 mRNA and protein in invasive CxCa were 4-fold (P<0.05) and 2-fold (P<0.01), respectively, stronger than those in normal subjects. The immunostaining of CHI3L1 was positively correlated with VEGF expression (P=0.0019) and microvessel density (P=0.0110). Moreover, CHI3L1 expression was also positively associated with cancer metastasis (P=0.011). The data suggest the crucial role of CHI3L1 by promoting angiogenesis, which may contribute to the development and progression of CxCa. The findings help establish CHI3L1 as a prognostic biomarker and therapeutic target for CxCa patients.
authors.affiliation: Biomedical Sciences, Graduate School, Khon Kaen University, Khon Kaen 40002, Thailand.
authors.forename: Nipaporn
authors.initials: N
authors.lastname: Ngernyuang
authors.affiliation: Departments of Molecular and Cellular Biology, University of Massachusetts Amherst, Amherst, MA 01003, USA.
authors.forename: Ralph A
authors.initials: RA
authors.lastname: Francescone
authors.affiliation: Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand.
authors.forename: Patcharee
authors.initials: P
authors.lastname: Jearanaikoon
authors.affiliation: Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand.
authors.forename: Jureerut
authors.initials: J
authors.lastname: Daduang
authors.affiliation: Department of Obstetrics and Gynecology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
authors.forename: Amornrat
authors.initials: A
authors.lastname: Supoken
authors.affiliation: Department of Veterinary and Animal Sciences, University of Massachusetts Amherst, MA 01003, USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Yan
authors.affiliation: Departments of Molecular and Cellular Biology, University of Massachusetts Amherst, Amherst, MA 01003, USA; Department of Veterinary and Animal Sciences, University of Massachusetts Amherst, MA 01003, USA.
authors.forename: Rong
authors.initials: R
authors.lastname: Shao
authors.affiliation: Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand. Electronic address: temduang@kku.ac.th.
authors.forename: Temduang
authors.initials: T
authors.lastname: Limpaiboon
mesh_terms: D054392:Adipokines / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D017209:Apoptosis / Q000502:physiology; D048708:Cell Growth Processes / Q000502:physiology; D045744:Cell Line, Tumor; D000071451:Chitinase-3-Like Protein 1; D018450:Disease Progression; D005260:Female; D006367:HeLa Cells; D006801:Humans; D037102:Lectins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D009389:Neovascularization, Pathologic / Q000378:metabolism / Q000473:pathology; D011379:Prognosis; D002583:Uterine Cervical Neoplasms / Q000098:blood supply* / Q000378:metabolism* / Q000473:pathology
chemical_list: D054392:Adipokines; C084533:CHI3L1 protein, human; D000071451:Chitinase-3-Like Protein 1; D037102:Lectins
keywords: Angiogenesis; Biomarkers; Cervical carcinoma; Chitinase 3 like 1; Tumor development