title: Characterization of bla(OxA-23) gene regions in isolates of Acinetobacter baumannii.
abstract: OBJECTIVE
  To investigate the characterization of bla(OxA-23) gene regions in isolates of Acinetobacter baumannii from Taizhou Municipal Hospital.
  
  
  METHODS
  Fifty-nine non-repetitive, multiresistant (including imipenem-resistant) isolates of A. baumannii were recovered from clinical infections in hospitalized patients from January 2010 to August 2011 in Taizhou Municipal Hospital (affiliated with Taizhou University) in China. These isolates were genotyped using pulsed-field gel electrophoresis (PFGE). bla(OxA-23) β-lactamase and associated genetic structures were analyzed using polymerase chain reaction (PCR), and recombination plasmids were analyzed by BamHI- or SacI- restriction enzyme digestion; predicted promoter structures of bla(OxA-23) genes were determined and compared using protein-protein BLAST analysis.
  
  
  RESULTS
  Fifteen out of 59 isolates expressing imipenem-resistant A. baumannii clinical isolates acquired either a bla(OxA-23) β-lactamase gene. A new gene cluster (ISAba1-bla(OxA-23)-AMP) with three previously identified transposons (Tn2006, Tn2007, and Tn2008) and one previously identified gene cluster (ISAba1- bla(OxA-23)) was found in the isolates. Recombination plasmids were analyzed by restriction enzyme digestion.
  
  
  CONCLUSIONS
  Our results indicate that pattern A was the most prevalent molecular type based on PFGE, and that different clones might be widespread with a majority of ISAba1-bla(OxA-23) clonal lineages in the 15 PCR positive isolates of A. baumannii in the hospital.
authors.affiliation: Department of Clinical Laboratory Medicine, Medical College of Taizhou University Affiliated Taizhou Municipal Hospital, 381 East Road of Zhongshan of Jiaojiang District in Taizhou, Taizhou 318000, Zhejiang Province, PRC. Electronic address: wdgtzs@163.com.
authors.forename: Dongguo
authors.initials: D
authors.lastname: Wang
authors.affiliation: Department of Urology Surgery, Medical College of Taizhou University Affiliated Taizhou Municipal Hospital, Taizhou, Zhejiang Province, PRC. Electronic address: ydl6737@163.com.
authors.forename: Dongliang
authors.initials: D
authors.lastname: Yan
authors.affiliation: Department of Infection, Medical College of Taizhou University Affiliated Taizhou Municipal Hospital, Taizhou, Zhejiang Province, PRC.
authors.forename: Wei
authors.initials: W
authors.lastname: Hou
authors.affiliation: The State Key Laboratory of Industry Microbiology, Tianjin Science and Technology University, Tianjin City, PRC.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Zeng
authors.affiliation: Department of Laboratory Medicine, Medical College of Taizhou University, Taizhou, Zhejiang Province, PRC.
authors.forename: Yongxiao
authors.initials: Y
authors.lastname: Qi
authors.affiliation: Department of Laboratory Medicine, Medical College of Taizhou University, Taizhou, Zhejiang Province, PRC.
authors.forename: Jiayu
authors.initials: J
authors.lastname: Chen
mesh_terms: D000151:Acinetobacter Infections / Q000453:epidemiology / Q000382:microbiology*; D040981:Acinetobacter baumannii / Q000145:classification / Q000201:enzymology* / Q000235:genetics* / Q000302:isolation & purification; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002681:China / Q000453:epidemiology; D016000:Cluster Analysis; D003428:Cross Infection / Q000453:epidemiology / Q000382:microbiology; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D005838:Genotype; D006785:Hospitals, University; D006801:Humans; D008826:Microbial Sensitivity Tests; D017720:Molecular Epidemiology; D058889:Molecular Typing; D005810:Multigene Family; D010957:Plasmids; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; C466679:beta-lactamase OXA-23; D001618:beta-Lactamases
keywords: Genetic structure; ISAba1- bla(OxA-23)-AMP; ISAba1-bla(OxA-23); Tn2006; Tn2007; Tn2008; bla(OxA-23) genes