title: Impact of hexavalent chromium on mammalian cell bioenergetics: phenotypic changes, molecular basis and potential relevance to chromate-induced lung cancer.
abstract: Occupational exposure to hexavalent chromium [Cr(VI)] has been firmly associated with the development of several pathologies, notably lung cancer. According to the current paradigm, the evolution of normal cells to a neoplastic state is accompanied by extensive metabolic reprogramming, namely at the level of energy-transducing processes. Thus, a complete understanding of the molecular basis of Cr(VI)-induced lung cancer must encompass the elucidation of the impact of Cr(VI) on metabolism. Research in this area is still in its infancy. Nonetheless, Cr(VI)-induced metabolic phenotypes are beginning to emerge. Specifically, it is now well documented that Cr(VI) exposure inhibits respiration and negatively affects the cellular energy status. Furthermore, preliminary results suggest that it also upregulates glucose uptake and lactic acid fermentation. From a mechanistic point of view, there is evidence that Cr(VI) exposure can interfere with energy transducing pathways at different levels, namely gene expression, intracellular protein levels and/or protein function. Loss of thiol redox control likely plays a key role in these processes. The transcriptional networks that control energy transduction can likewise be affected. Data also suggest that Cr(VI) exposure might compromise energy transducing processes through changes in the intracellular pools of their substrates. This article reviews, for the first time, the information available on Cr(VI) impact on mammalian cell bioenergetics. It aims to provide a framework for the understanding of the role played by bioenergetics in Cr(VI)-induced carcinogenesis and is also intended as a guide for future research efforts in this area.
authors.affiliation: Unidade de Química-Física Molecular and Departamento de Ciências da Vida (Bioquímica), Faculdade de Ciências e Tecnologia, Universidade de Coimbra, Rua dos Estudos, 3001-401, Coimbra, Portugal.
authors.forename: P L
authors.initials: PL
authors.lastname: Abreu
authors.forename: L M R
authors.initials: LM
authors.lastname: Ferreira
authors.forename: M C
authors.initials: MC
authors.lastname: Alpoim
authors.forename: A M
authors.initials: AM
authors.lastname: Urbano
mesh_terms: D000818:Animals; D002840:Chromates / Q000633:toxicity*; D002857:Chromium / Q000633:toxicity*; D004734:Energy Metabolism / Q000187:drug effects*; D015870:Gene Expression / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D008175:Lung Neoplasms / Q000139:chemically induced* / Q000473:pathology; D053858:Metabolic Networks and Pathways / Q000187:drug effects; D016273:Occupational Exposure; D018384:Oxidative Stress; D010641:Phenotype
chemical_list: D002840:Chromates; D002857:Chromium