title: [Determination of lutein in infant formula milk powder using ultra-high performance liquid chromatography].
abstract: An ultra-high performance liquid chromatography (U-HPLC) method for the determination of lutein in the infant formula milk powder was developed. The sample was extracted with acetone and defatted using freezing centrifugation method. The U-HPLC separation was achieved using a YMC Carotenoid C30 column (150 mm x 4.6 mm, 3 microm) with the mixture of methanol/methyl tert-butyl ether (70: 30, v/v) as the mobile phase under isocratic elution. The flow rate was 0.5 mL/min and the column oven temperature was 25 degrees C. The injection volume was 5 microL. It was detected on a photodiode array detector at a wavelength of 445 nm. The results showed that the linear range was 20-500 microg/L (r = 0.9999), and the limit of quantification was 20 microg/L. The mean recoveries of lutein varied from 97.9% to 104.4% spiked at 50, 250 and 2,000 microg/kg. The established method is simple, accurate and sensitive for the rapid determination of lutein in infant formula milk powder.
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.forename: Junrong
authors.initials: J
authors.lastname: Huang
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Feng
authors.initials: F
authors.lastname: Feng
authors.forename: Yun
authors.initials: Y
authors.lastname: Ling
authors.forename: Xiaogang
authors.initials: X
authors.lastname: Chu
authors.forename: Hongliang
authors.initials: H
authors.lastname: Li
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005504:Food Analysis; D041943:Infant Formula / Q000737:chemistry*; D014975:Lutein / Q000032:analysis*; D008892:Milk / Q000737:chemistry*; D011208:Powders
chemical_list: D011208:Powders; D014975:Lutein