title: Terminal protection of small molecule-linked DNA for small molecule-protein interaction assays.
abstract: Methods for the detection of specific interactions between diverse proteins and various small-molecule ligands are of significant importance in understanding the mechanisms of many critical physiological processes of organisms. The techniques also represent a major avenue to drug screening, molecular diagnostics, and public safety monitoring. Terminal protection assay of small molecule-linked DNA is a demonstrated novel methodology which has exhibited great potential for the development of simple, sensitive, specific and high-throughput methods for the detection of small molecule-protein interactions. Herein, we review the basic principle of terminal protection assay, the development of associated methods, and the signal amplification strategies adopted for performance improving in small molecule-protein interaction assay.
authors.affiliation: State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China. S12111016@hnu.edu.cn.
authors.forename: Cui
authors.initials: C
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China. zhanwu@hnu.edu.cn.
authors.forename: Zhan
authors.initials: Z
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China. haotang@hnu.edu.cn.
authors.forename: Hao
authors.initials: H
authors.lastname: Tang
authors.affiliation: State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China. tanglijuan@hnu.edu.cn.
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Tang
authors.affiliation: State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China. rqyu@hnu.edu.cn.
authors.forename: Ru-Qin
authors.initials: RQ
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China. jianhuijiang@hnu.edu.cn.
authors.forename: Jian-Hui
authors.initials: JH
authors.lastname: Jiang
mesh_terms: D004247:DNA / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005092:Exonucleases / Q000378:metabolism; D006801:Humans; D008024:Ligands; D021141:Nucleic Acid Amplification Techniques; D057089:Pathology, Molecular; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs / Q000502:physiology*
chemical_list: D004268:DNA-Binding Proteins; D008024:Ligands; D004247:DNA; D005092:Exonucleases