title: Production of somatic cell nuclear transfer embryos using in vitro-grown and in vitro-matured oocytes in rabbits.
abstract: We examined growing oocytes collected from follicles remaining in superovulated rabbit ovaries, that were grown (in vitro growth, IVG) and matured (in vitro maturation, IVM) in vitro. We produced somatic cell nuclear transfer (SCNT) embryos using the mature oocytes and examined whether these embryos have the ability to develop to the blastocyst stage. In addition, we examined the effects of trichostatin A (TSA), a histone deacetylase inhibitor (HDACi), on the developmental competence of SCNT embryos derived from IVG-IVM oocytes. After growth for 7 days and maturation for 14-16 h in vitro, the growing oocytes reached the metaphase II stage (51.4%). After SCNT, these reconstructed embryos reached the blastocyst stage (20%). Furthermore, the rate of development to the blastocyst stage and the number of cells in the blastocysts in SCNT embryos derived from IVG-IVM oocytes were significantly higher for TSA-treated embryos compared with TSA-untreated embryos (40.6 versus 21.4% and 353.1 ± 59.1 versus 202.5 ± 54.6, P < 0.05). These results indicate that rabbit SCNT embryos using IVG-IVM oocytes have the developmental competence to reach the blastocyst stage.
authors.affiliation: Taniguchi Hospital,1-5-20 Onishi,Izumisano,Osaka 598-0043,Japan.
authors.forename: Hironobu
authors.initials: H
authors.lastname: Sugimoto
authors.affiliation: Division of Biological Science,Graduate School of Biology-oriented Science and Technology,Kinki University,930 Nishimitani,Kinokawa,Wakayama 649-6493,Japan.
authors.forename: Yuta
authors.initials: Y
authors.lastname: Kida
authors.affiliation: Division of Biological Science,Graduate School of Biology-oriented Science and Technology,Kinki University,930 Nishimitani,Kinokawa,Wakayama 649-6493,Japan.
authors.forename: Noriyoshi
authors.initials: N
authors.lastname: Oh
authors.affiliation: Kitayama Labes Co. Ltd.,Ina Research Laboratory,Ina,Nagano 396-0021,Japan.
authors.forename: Kensaku
authors.initials: K
authors.lastname: Kitada
authors.affiliation: Division of Biological Science,Graduate School of Biology-oriented Science and Technology,Kinki University,930 Nishimitani,Kinokawa,Wakayama 649-6493,Japan.
authors.forename: Kazuya
authors.initials: K
authors.lastname: Matsumoto
authors.affiliation: Division of Biological Science,Graduate School of Biology-oriented Science and Technology,Kinki University,930 Nishimitani,Kinokawa,Wakayama 649-6493,Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Saeki
authors.affiliation: Taniguchi Hospital,1-5-20 Onishi,Izumisano,Osaka 598-0043,Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Taniguchi
authors.affiliation: Department of Genetic Development,Kinki University,Wakayama 649-6493,Japan.
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Hosoi
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001755:Blastocyst / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D004622:Embryo, Mammalian / Q000187:drug effects; D005260:Female; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology; D006877:Hydroxamic Acids / Q000494:pharmacology*; D059471:In Vitro Oocyte Maturation Techniques / Q000379:methods*; D008677:Metaphase; D053652:Nuclear Transfer Techniques; D009865:Oocytes / Q000166:cytology / Q000502:physiology; D011817:Rabbits
chemical_list: D056572:Histone Deacetylase Inhibitors; D006877:Hydroxamic Acids; C012589:trichostatin A
keywords: Follicle; In vitro growth; Nuclear transfer; Rabbit; Trichostatin A