title: Long-term performance and microbial ecology of a two-stage PN-ANAMMOX process treating mature landfill leachate.
abstract: Long-term performance of a two-stage partial nitritation (PN)-anaerobic ammonium oxidation (ANAMMOX) process treating mature landfill leachate was investigated. Stable partial nitritation performance was achieved in a sequencing batch reactor (SBR) using endpoint pH control, providing an effluent with a ratio of NO2(-)-N/NH4(+)-N at 1.23 ± 0.23. High rate nitrogen removal over 4 kg N/m(3)/d was observed in the ANAMMOX reactor in the first three months. However, during long-term operation, the ANAMMOX reactor can only stably operate under nitrogen load of 1 kg N/m(3)/d, with 85 ± 1% of nitrogen removal. The ammonium oxidizing bacteria (AOB) in the PN-SBR were mainly affiliated to Nitrosomonas sp. IWT514, Nitrosomonas eutropha and Nitrosomonas eutropha, the anaerobic ammonium oxidizing bacteria (AnAOB) in the ANAMMOX reactor were mainly affiliated to Kuenenia stuttgartiensis.
authors.affiliation: College of Environment and Energy, South China University of Technology, Guangzhou Higher Education Mega Center, Guangzhou 510006, PR China.
authors.forename: Huosheng
authors.initials: H
authors.lastname: Li
authors.affiliation: College of Environment and Energy, South China University of Technology, Guangzhou Higher Education Mega Center, Guangzhou 510006, PR China; Guizhou Academy of Sciences, Shanxi Road 1, Guiyang 550001, PR China; State Key Laboratory of Subtropical Building Sciences, South China University of Technology, Guangzhou 510641, PR China; Key Laboratory of Environmental Protection and Eco-remediation of Guangdong Regular Higher Education Institutions, South China University of Technology, Guangzhou Higher Education Mega Center, Guangzhou 510006, PR China; College of Civil Engineering and Transportation, South China University of Technology, Guangzhou 510640, PR China. Electronic address: fesqzhou@scut.edu.cn.
authors.forename: Shaoqi
authors.initials: S
authors.lastname: Zhou
authors.affiliation: College of Civil Engineering and Transportation, South China University of Technology, Guangzhou 510640, PR China.
authors.forename: Weihao
authors.initials: W
authors.lastname: Ma
authors.affiliation: College of Environment and Energy, South China University of Technology, Guangzhou Higher Education Mega Center, Guangzhou 510006, PR China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Huang
authors.affiliation: College of Civil Engineering and Transportation, South China University of Technology, Guangzhou 510640, PR China.
authors.forename: Guotao
authors.initials: G
authors.lastname: Huang
authors.affiliation: College of Environment and Energy, South China University of Technology, Guangzhou Higher Education Mega Center, Guangzhou 510006, PR China. Electronic address: qinyujie@scut.edu.cn.
authors.forename: Yujie
authors.initials: Y
authors.lastname: Qin
authors.affiliation: College of Civil Engineering and Transportation, South China University of Technology, Guangzhou 510640, PR China.
authors.forename: Bin
authors.initials: B
authors.lastname: Xu
authors.affiliation: College of Civil Engineering and Transportation, South China University of Technology, Guangzhou 510640, PR China.
authors.forename: Hai
authors.initials: H
authors.lastname: Ouyang
mesh_terms: D000388:Air; D064751:Ammonium Compounds / Q000378:metabolism*; D000693:Anaerobiosis; D001419:Bacteria / Q000378:metabolism*; D001673:Biodegradation, Environmental; D019149:Bioreactors / Q000382:microbiology; D006863:Hydrogen-Ion Concentration; D058465:Nitrification; D009584:Nitrogen / Q000302:isolation & purification; D010084:Oxidation-Reduction; D010802:Phylogeny; D013696:Temperature; D014874:Water Pollutants, Chemical / Q000302:isolation & purification*; D018508:Water Purification / Q000379:methods*
chemical_list: D064751:Ammonium Compounds; D014874:Water Pollutants, Chemical; D009584:Nitrogen
keywords: ANAMMOX; Landfill leachate; Nitrogen removal; Partial nitritation; Wastewater treatment