title: Differential effect of beetroot bread on postprandial DBP according to Glu298Asp polymorphism in the eNOS gene: a pilot study.
abstract: Our objective was to investigate whether the presence of Glu298Asp polymorphism in the endothelial NO synthase (eNOS) gene differentially affects the postprandial blood pressure response to dietary nitrate-rich beetroot bread. A randomised, single-blind, controlled, crossover acute pilot study was performed in 14 healthy men (mean age: 34±9 years) who were retrospectively genotyped for Glu298Asp polymorphism (7GG; T carriers 7). Volunteers were randomised to receive 200 g beetroot-enriched bread (1.1 mmol nitrate) or control bread (no beetroot; 0.01 mmol nitrate) on two separate occasions 10 days apart. Baseline and incremental area under the curve of blood pressure and NOx (nitrate/nitrite) were measured for a 6-h postprandial period. A treatment × genotype interaction was observed for diastolic blood pressure (P<0.02), which was significantly lower in T carriers (P<0.01) after consumption of beetroot bread compared with control bread. No significant differences were observed in the GG group. The beneficial diastolic blood pressure reduction was observed only in the T carriers of the Glu298Asp polymorphism in the eNOS gene after consumption of nitrate-rich beetroot bread. These data require confirmation in a larger population group.
authors.affiliation: 1] Hugh Sinclair Unit of Human Nutrition, Department of Food and Nutritional Sciences, University of Reading, Whiteknights, Reading, Berkshire, UK [2] Institute for Cardiovascular and Metabolic Research (ICMR), University of Reading, Whiteknights, Reading, Berkshire, UK.
authors.forename: D A
authors.initials: DA
authors.lastname: Hobbs
authors.affiliation: Eccentricities Ltd, Camberley, Surrey, UK.
authors.forename: T W
authors.initials: TW
authors.lastname: George
authors.affiliation: 1] Hugh Sinclair Unit of Human Nutrition, Department of Food and Nutritional Sciences, University of Reading, Whiteknights, Reading, Berkshire, UK [2] Institute for Cardiovascular and Metabolic Research (ICMR), University of Reading, Whiteknights, Reading, Berkshire, UK.
authors.forename: J A
authors.initials: JA
authors.lastname: Lovegrove
mesh_terms: D000293:Adolescent; D000328:Adult; D001794:Blood Pressure; D001939:Bread; D006801:Humans; D008297:Male; D008875:Middle Aged; D009566:Nitrates / Q000032:analysis; D052250:Nitric Oxide Synthase Type III / Q000235:genetics*; D010865:Pilot Projects; D020641:Polymorphism, Single Nucleotide; D014675:Vegetables / Q000737:chemistry*
chemical_list: D009566:Nitrates; D052250:Nitric Oxide Synthase Type III