title: Functional genomic analysis of the periodic transcriptome in the developing Drosophila wing.
abstract: The eukaryotic cell cycle, driven by both transcriptional and posttranslational mechanisms, is the central molecular oscillator underlying tissue growth throughout animals. Although genome-wide studies have investigated cell-cycle-associated transcription in unicellular systems, global patterns of periodic transcription in multicellular tissues remain largely unexplored. Here we define the cell-cycle-associated transcriptome of the developing Drosophila wing epithelium and compare it with that of cultured Drosophila S2 cells, revealing a core set of periodic genes and a surprising degree of context specificity in periodic transcription. We further employ RNAi-mediated phenotypic profiling to define functional requirements for more than 300 periodic genes, with a focus on those required for cell proliferation in vivo. Finally, we investigate uncharacterized genes required for interkinetic nuclear migration. Combined, these findings provide a global perspective on cell-cycle control in vivo, and they highlight a critical need to understand the context-specific regulation of cell proliferation.
authors.affiliation: Stowers Institute for Medical Research, 1000 East 50(th) Street, Kansas City, MO 64110, USA.
authors.forename: Liang
authors.initials: L
authors.lastname: Liang
authors.affiliation: Stowers Institute for Medical Research, 1000 East 50(th) Street, Kansas City, MO 64110, USA.
authors.forename: Jeffrey S
authors.initials: JS
authors.lastname: Haug
authors.affiliation: Stowers Institute for Medical Research, 1000 East 50(th) Street, Kansas City, MO 64110, USA.
authors.forename: Chris W
authors.initials: CW
authors.lastname: Seidel
authors.affiliation: Stowers Institute for Medical Research, 1000 East 50(th) Street, Kansas City, MO 64110, USA; Department of Anatomy and Cell Biology, Kansas University Medical Center, 3901 Rainbow Boulevard, Kansas City, KS 66160, USA. Electronic address: mg2@stowers.org.
authors.forename: Matthew C
authors.initials: MC
authors.lastname: Gibson
mesh_terms: D000818:Animals; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D004330:Drosophila / Q000196:embryology / Q000235:genetics* / Q000378:metabolism; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D050437:Genes, Developmental; D049750:Genome, Insect; D062085:RNA, Long Noncoding / Q000235:genetics / Q000378:metabolism; D059467:Transcriptome; D014921:Wings, Animal / Q000166:cytology / Q000196:embryology*
chemical_list: D018797:Cell Cycle Proteins; D062085:RNA, Long Noncoding