title: Normal ranges and variability of novel urinary renal biomarkers in Sprague-Dawley Rats: comparison of constitutive values between males and females and across assay platforms.
abstract: Differences were examined between male and female Sprague-Dawley rats in basal levels of a wide range of urinary biomarkers, including 7 recently qualified biomarkers. The data were generated from urine samples collected on 3 occasions from untreated rats included in a study of the effect of gentamicin nephrotoxicity on urinary renal biomarkers, reported in a companion article in this journal (Gautier et al. 2014). The performance of multiple assays (9 singleplex assays and 2 multiplex platforms from Rules Based Medicine [RBM] and Meso Scale Discovery [MSD]) was evaluated, and normal ranges and variability estimates were derived. While variability was generally greater on the RBM platform than other assays, the more striking difference in the results from different assays was in magnitude. Where differences were observed between assays for an individual biomarker, they were seen in both sexes and consistent across samples collected at different time points. Differences of up to 15-fold were observed for some biomarker values between assays indicating that results generated using different assays should not be compared. For 8 biomarkers, there was compelling evidence for a sex difference. Baseline values in males were significantly higher than in females for total protein, β2-microglobulin, clusterin, cystatin-C, glutathione-S-transferase (GST-α), tissue inhibitor of metalloproteinases (TIMP-1), and vascular endothelial growth factor (VEGF); female values were significantly higher than that of males for albumin. The largest sex differences (male greater than female by 2- to 11-fold) were seen with β2-microglobulin, GST-α, and TIMP-1. These data add substantially to the limited body of knowledge in this area and provide a useful framework for evaluation of the potential relevance of sex differences in the diagnostic performance of these biomarkers.
authors.affiliation: Sanofi R&D, Paris, France jean-charles.gautier@sanofi.com.
authors.forename: Jean-Charles
authors.initials: JC
authors.lastname: Gautier
authors.affiliation: Sanofi R&D, Paris, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Gury
authors.affiliation: Pfizer, Pearl River, New York, USA.
authors.forename: Magali
authors.initials: M
authors.lastname: Guffroy
authors.affiliation: Sanofi R&D, Bridgewater, New Jersey, USA.
authors.forename: Richard
authors.initials: R
authors.lastname: Khan-Malek
authors.affiliation: Sanofi R&D, Bridgewater, New Jersey, USA.
authors.forename: David
authors.initials: D
authors.lastname: Hoffman
authors.affiliation: ILSI Health and Environmental Sciences Institute, Washington, District of Columbia, USA.
authors.forename: Syril
authors.initials: S
authors.lastname: Pettit
authors.affiliation: Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK.
authors.forename: Ernie
authors.initials: E
authors.lastname: Harpur
mesh_terms: D000818:Animals; D001681:Biological Assay / Q000379:methods*; D015415:Biomarkers / Q000652:urine*; D051152:Clusterin / Q000235:genetics / Q000378:metabolism; D055316:Cystatin C / Q000235:genetics / Q000378:metabolism; D005260:Female; D005839:Gentamicins / Q000008:administration & dosage / Q000009:adverse effects; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012016:Reference Values; D015203:Reproducibility of Results; D012737:Sex Factors; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000235:genetics / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism; D001613:beta 2-Microglobulin / Q000235:genetics / Q000378:metabolism
chemical_list: D015415:Biomarkers; D051152:Clusterin; D055316:Cystatin C; D005839:Gentamicins; D007527:Isoenzymes; C576839:TIMP1 protein, rat; D019715:Tissue Inhibitor of Metalloproteinase-1; D042461:Vascular Endothelial Growth Factor A; D001613:beta 2-Microglobulin; C467498:vascular endothelial growth factor A, rat; D005982:Glutathione Transferase; C119995:glutathione S-transferase alpha
keywords: Sprague-Dawley rats.; kidney; normal ranges; sex differences; singleplex and multiplexed assays; urinary biomarkers