title: A comparison of Helicobacter pylori and non-Helicobacter pylori Helicobacter spp. Binding to canine gastric mucosa with defined gastric glycophenotype.
abstract: BACKGROUND
  The gastric mucosa of dogs is often colonized by non-Helicobacter pylori helicobacters (NHPH), while H. pylori is the predominant gastric Helicobacter species in humans. The colonization of the human gastric mucosa by H. pylori is highly dependent on the recognition of host glycan receptors. Our goal was to define the canine gastric mucosa glycophenotype and to evaluate the capacity of different gastric Helicobacter species to adhere to the canine gastric mucosa.
  
  
  METHODS
  The glycosylation profile in body and antral compartments of the canine gastric mucosa, with focus on the expression of histo-blood group antigens was evaluated. The in vitro binding capacity of FITC-labeled H. pylori and NHPH to the canine gastric mucosa was assessed in cases representative of the canine glycosylation pattern.
  
  
  RESULTS
  The canine gastric mucosa lacks expression of type 1 Lewis antigens and presents a broad expression of type 2 structures and A antigen, both in the surface and glandular epithelium. Regarding the canine antral mucosa, H. heilmannii s.s. presented the highest adhesion score whereas in the body region the SabA-positive H. pylori strain was the strain that adhered more.
  
  
  CONCLUSIONS
  The canine gastric mucosa showed a glycosylation profile different from the human gastric mucosa suggesting that alternative glycan receptors may be involved in Helicobacter spp. binding. Helicobacter pylori and NHPH strains differ in their ability to adhere to canine gastric mucosa. Among the NHPH, H. heilmannii s.s. presented the highest adhesion capacity in agreement with its reported colonization of the canine stomach.
authors.affiliation: Department of Pathology and Molecular Immunology of the Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, Rua Jorge Viterbo Ferreira nr. 228, Porto, 4050-313, Portugal; Institute of Molecular Pathology and Immunology of the University of Porto (IPATIMUP), Rua Dr. Roberto Frias s/n, Porto, 4200-465, Portugal.
authors.forename: Irina
authors.initials: I
authors.lastname: Amorim
authors.forename: Daniela P
authors.initials: DP
authors.lastname: Freitas
authors.forename: Ana
authors.initials: A
authors.lastname: Magalhães
authors.forename: Fátima
authors.initials: F
authors.lastname: Faria
authors.forename: Célia
authors.initials: C
authors.lastname: Lopes
authors.forename: Augusto M
authors.initials: AM
authors.lastname: Faustino
authors.forename: Annemieke
authors.initials: A
authors.lastname: Smet
authors.forename: Freddy
authors.initials: F
authors.lastname: Haesebrouck
authors.forename: Celso A
authors.initials: CA
authors.lastname: Reis
authors.forename: Fátima
authors.initials: F
authors.lastname: Gärtner
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D004285:Dogs; D005753:Gastric Mucosa / Q000737:chemistry* / Q000382:microbiology*; D006031:Glycosylation; D016998:Helicobacter / Q000502:physiology*; D006649:Histocompatibility Antigens / Q000737:chemistry*; D011134:Polysaccharides / Q000032:analysis*
chemical_list: D006649:Histocompatibility Antigens; D011134:Polysaccharides
keywords: Canine gastric mucosa; Helicobacter pylori; bacterial adhesion; histo blood group antigens; non-Helicobacter pylori helicobacters