title: Analysis, physiological and clinical significance of 12-HETE: a neglected platelet-derived 12-lipoxygenase product.
abstract: While the importance of cyclooxygenase (COX) in platelet function has been amply elucidated, the identification of the role of 12-lipoxygenase (12-LOX) and of its stable metabolite, 12-hydroxyeicosatretraenoic acid (12-HETE), has not been clarified as yet. Many studies have analysed the implications of 12-LOX products in different pathological disorders but the information obtained from these works is controversial. Several analytical methods have been developed over the years to simultaneously detect eicosanoids, and specifically 12-HETE, in different biological matrices, essentially enzyme-linked immunosorbent assays (ELISA), radioimmunoassays (RIA), high performance liquid chromatography (HPLC) and mass spectrometry coupled with both gas and liquid chromatography methods (GC- and LC-MS). This review is aimed at summarizing the up to now known physiological and clinical features of 12-HETE together with the analytical methods used for its determination, focusing on the critical issues regarding its measurement.
authors.affiliation: Centro Cardiologico Monzino-IRCCS, Milan, Italy.
authors.forename: Benedetta
authors.initials: B
authors.lastname: Porro
authors.affiliation: Centro Cardiologico Monzino-IRCCS, Milan, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Songia
authors.affiliation: Centro Cardiologico Monzino-IRCCS, Milan, Italy.
authors.forename: Isabella
authors.initials: I
authors.lastname: Squellerio
authors.affiliation: Centro Cardiologico Monzino-IRCCS, Milan, Italy; Università degli Studi di Milano, Dipartimento di Scienze Farmacologiche e Biomolecolari, Milan, Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: Tremoli
authors.affiliation: Centro Cardiologico Monzino-IRCCS, Milan, Italy; Dipartimento di Scienze Cliniche e di Comunità, Milan, Italy. Electronic address: viviana.cavalca@unimi.it.
authors.forename: Viviana
authors.initials: V
authors.lastname: Cavalca
mesh_terms: D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid / Q000032:analysis* / Q000378:metabolism*; D000818:Animals; D001792:Blood Platelets / Q000737:chemistry / Q000166:cytology / Q000378:metabolism* / Q000473:pathology; D002318:Cardiovascular Diseases / Q000378:metabolism / Q000473:pathology; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D009369:Neoplasms / Q000378:metabolism / Q000473:pathology
chemical_list: D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid
keywords: 12-HETE; 12-LOX clinical implication; Analytical method; Arachidonic acid metabolism; Platelet