title: Effect of superovulation on uterine and serum biochemical parameters and its potential association with transferable embryos in Holstein dairy cows.
abstract: The objective of this study was to determine the effects of superovulation (SOV) on serum and uterine biochemical parameters, uterine bacteriology and cytology and number of transferable embryos (TE). Dairy cows were placed on a Presynch/CIDR Synch protocol. The SOV group was superovulated, induced in estrus, and inseminated, whereas the control group was induced in estrus and inseminated without SOV. Uterine bacteriology and cytology and uterine and serum biochemical parameters were measured at day 7 of the estrous cycle to start the SOV protocol, as well as on the day of embryo recovery (DER). The SOV group produced 7.5 ± 6.7 oocytes/embryos, of which 3.4 ± 4.7 were TE. Serum urea and E2 and uterine Glu, CK, LDH, TP, P4 and PGFM in the control group and serum P4 and PGFM and uterine LDH and PGFM in the SOV group were significantly higher (p < 0.01) at DER than day 7. At DER, uterine urea, LDH, PGFM and TP and serum urea, LDH, PGFM, and P4 concentrations were higher (p < 0.01) in the SOV group than the control. There was no significant variation in uterine bacteriology or cytology. Overall, these results infer that SOV affects both serum profile and uterine secretions, and that these changes may influence the number of TE.
authors.affiliation: Departement of Veterinary Biomedicine, Faculty of Veterinary Medicine, University of Montreal, Saint-Hyacinthe J2S 7C6, Canada.
authors.forename: Hasina Santatriniaina
authors.initials: HS
authors.lastname: Rasolomboahanginjatovo
authors.forename: Younès
authors.initials: Y
authors.lastname: Chorfi
authors.forename: Raynald
authors.initials: R
authors.lastname: Dupras
authors.forename: Louis
authors.initials: L
authors.lastname: Mills
authors.forename: Réjean
authors.initials: R
authors.lastname: Lefebvre
mesh_terms: D000818:Animals; D001774:Blood Chemical Analysis / Q000662:veterinary; D002417:Cattle / Q000097:blood / Q000196:embryology* / Q000502:physiology*; D004624:Embryo Transfer / Q000662:veterinary; D047108:Embryonic Development; D030762:Estrous Cycle; D005260:Female; D013480:Superovulation; D014599:Uterus / Q000737:chemistry* / Q000166:cytology / Q000382:microbiology*
keywords: biochemical parameters; dairy cows; superovulation; transferable embryos