title: Introduction to super-resolution microscopy.
abstract: In this review, we introduce the principles of spatial resolution improvement in super-resolution microscopies that were recently developed. These super-resolution techniques utilize the interaction of light and fluorescent probes in order to break the diffraction barrier that limits spatial resolution. The imaging property of each super-resolution technique is also compared with the corresponding conventional one. Typical applications of the super-resolution techniques in biological research are also introduced.
authors.affiliation: Department of Applied Physics Immunology Frontier Research Center, Osaka University, Suita, Osaka, Japan.
authors.forename: Masahito
authors.initials: M
authors.lastname: Yamanaka
authors.affiliation: Department of Applied Physics Immunology Frontier Research Center, Osaka University, Suita, Osaka, Japan.
authors.forename: Nicholas I
authors.initials: NI
authors.lastname: Smith
authors.affiliation: Department of Applied Physics Immunology Frontier Research Center, Osaka University, Suita, Osaka, Japan fujita@ap.eng.osaka-u.ac.jp.
authors.forename: Katsumasa
authors.initials: K
authors.lastname: Fujita
mesh_terms: D000818:Animals; D006801:Humans; D008853:Microscopy / Q000379:methods*; D018613:Microscopy, Confocal / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods; D015388:Organelles / Q000648:ultrastructure
keywords: PALM; SAX; SIM; STED; STORM; Super-resolution microscopy