title: Streptomyces hokutonensis sp. nov., a novel actinomycete isolated from the strawberry root rhizosphere.
abstract: A polyphasic approach was used to determine the taxonomic position of actinomycete strain R1-NS-10(T), which was isolated from a sample of strawberry root rhizosphere obtained from Hokuto, Yamanashi, Japan. Strain R1-NS-10(T) was Gram-staining-positive and aerobic, and formed brownish-white aerial mycelia and grayish-brown substrate mycelia on ISP-2 medium. The strain grew in the presence of 0-5% (w/v) NaCl and optimally grew without NaCl. The strain grew at pH 5-8, and the optimum for growth was pH 7. The optimal growth temperature was 30 °C, but the strain grew at 5-37 °C. Whole-cell hydrolysates of strain R1-NS-10(T) contained A2pm, galactose, mannose and rhamnose. The predominant menaquinones were MK-9(H6) and MK-9(H8). The major cellular fatty acids were anteiso-C15:0 and iso-C16:0. Comparative 16S rRNA gene sequence analysis revealed that strain R1-NS-10(T) was most closely related to Streptomyces prunicolor NBRC 13075(T) (99.4%). The draft genome sequences of both strains were determined for characterization of genome sequence-related parameters such as average nucleotide identity (ANI) and the diversity of secondary metabolite biosynthetic gene clusters. DNA-DNA hybridization (DDH) and ANI values for both strains were below the species delineation cutoff, and differences in physiological and biochemical characteristics differentiated strain R1-NS-10(T) from its closest phylogenetic relative. On the basis of these data, we propose that strain R1-NS-10(T) (=NBRC 108812(T)=KCTC 29186(T)) should be classified as the type strain of a novel Streptomyces species named Streptomyces hokutonensis sp. nov.
authors.affiliation: Division of Applied Biological Sciences, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Kofu, Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Yamamura
authors.affiliation: Division of Applied Biological Sciences, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Kofu, Japan.
authors.forename: Haruna
authors.initials: H
authors.lastname: Ashizawa
authors.affiliation: Biological Resource Center, National Institute of Technology and Evaluation, (NBRC), Kisarazu, Japan.
authors.forename: Moriyuki
authors.initials: M
authors.lastname: Hamada
authors.affiliation: Biological Resource Center, National Institute of Technology and Evaluation, (NBRC), Kisarazu, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Hosoyama
authors.affiliation: Biological Resource Center, National Institute of Technology and Evaluation, (NBRC), Kisarazu, Japan.
authors.forename: Hisayuki
authors.initials: H
authors.lastname: Komaki
authors.affiliation: Biological Resource Center, National Institute of Technology and Evaluation, (NBRC), Kisarazu, Japan.
authors.forename: Misa
authors.initials: M
authors.lastname: Otoguro
authors.affiliation: Biological Resource Center, National Institute of Technology and Evaluation, (NBRC), Kisarazu, Japan.
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Tamura
authors.affiliation: Division of Applied Biological Sciences, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Kofu, Japan.
authors.forename: Yukikazu
authors.initials: Y
authors.lastname: Hayashi
authors.affiliation: Division of Applied Biological Sciences, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Kofu, Japan.
authors.forename: Youji
authors.initials: Y
authors.lastname: Nakagawa
authors.affiliation: Division of Applied Biological Sciences, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Kofu, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Ohtsuki
authors.affiliation: Biological Resource Center, National Institute of Technology and Evaluation, (NBRC), Kisarazu, Japan.
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Fujita
authors.affiliation: Division of Applied Biological Sciences, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Kofu, Japan.
authors.forename: Sadaharu
authors.initials: S
authors.lastname: Ui
authors.affiliation: Division of Applied Biological Sciences, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Kofu, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Hayakawa
mesh_terms: D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D031985:Fragaria / Q000378:metabolism / Q000382:microbiology*; D007564:Japan; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D058441:Rhizosphere; D013302:Streptomyces / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S