title: Programmed degradation of DNA multilayer films.
abstract: The design and assembly of DNA multilayer films with programmable degradation properties are reported. The nanostructured DNA films are assembled through the layer-by-layer (LbL) assembly technique and can be programmed to degrade by subsequently introducing DNA strands of specific sequences. The strands preferentially hybridize to the building blocks that stabilize the film structure, causing the film to rearrange and degrade. The rate of degradation is influenced by both the availability and accessibility of the complementary DNA binding sites within the film, as well as the degree of crosslinking within the film. Similar results are obtained for DNA multilayer films assembled on planar and particle supports. This approach offers an avenue to tailor degradability features into DNA-based materials that may find application in the biosciences, in areas such as biosensing and drug delivery.
authors.affiliation: Department of Chemical and Biomolecular Engineering, The University of Melbourne, Victoria, 3010, Australia.
authors.forename: Lillian
authors.initials: L
authors.lastname: Lee
authors.forename: Angus P R
authors.initials: AP
authors.lastname: Johnston
authors.forename: Frank
authors.initials: F
authors.lastname: Caruso
mesh_terms: D001483:Base Sequence; D015374:Biosensing Techniques; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D016503:Drug Delivery Systems; D049329:Nanostructures / Q000737:chemistry / Q000648:ultrastructure; D036103:Nanotechnology; D009693:Nucleic Acid Hybridization; D058525:Quartz Crystal Microbalance Techniques; D012822:Silicon Dioxide
chemical_list: D012822:Silicon Dioxide; D004247:DNA