title: Comparative transcriptome analysis of two races of Heterodera glycines at different developmental stages.
abstract: The soybean cyst nematode, Heterodera glycines, is an important pest of soybeans. Although resistance is available against this nematode, selection for virulent races can occur, allowing the nematode to overcome the resistance of cultivars. There are abundant field populations, however, little is known about their genetic diversity. In order to elucidate the differences between races, we investigated the transcriptional diversity within race 3 and race 4 inbred lines during their compatible interactions with the soybean host Zhonghuang 13. Six different race-enriched cDNA libraries were constructed with limited nematode samples collected from the three sedentary stages, parasitic J2, J3 and J4 female, respectively. Among 689 putative race-enriched genes isolated from the six libraries with functional annotations, 92 were validated by quantitative RT-PCR (qRT-PCR), including eight putative effector encoding genes. Further race-enriched genes were validated within race 3 and race 4 during development in soybean roots. Gene Ontology (GO) analysis of all the race-enriched genes at J3 and J4 female stages showed that most of them functioned in metabolic processes. Relative transcript level analysis of 13 selected race-enriched genes at four developmental stages showed that the differences in their expression abundance took place at either one or more developmental stages. This is the first investigation into the transcript diversity of H. glycines races throughout their sedentary stages, increasing the understanding of the genetic diversity of H. glycines.
authors.affiliation: The Key Laboratory for Biology of Insect Pests and Plant Disease, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China; Department of Plant Pathology, China Agricultural University, Beijing, China.
authors.forename: Gaofeng
authors.initials: G
authors.lastname: Wang
authors.affiliation: The Key Laboratory for Biology of Insect Pests and Plant Disease, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Deliang
authors.initials: D
authors.lastname: Peng
authors.affiliation: Huzhou Modem Agricultural Biotechnology Innovation Center, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Huzhou, Zhejiang, China.
authors.forename: Bingli
authors.initials: B
authors.lastname: Gao
authors.affiliation: The Key Laboratory for Biology of Insect Pests and Plant Disease, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Wenkun
authors.initials: W
authors.lastname: Huang
authors.affiliation: The Key Laboratory for Biology of Insect Pests and Plant Disease, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Lingan
authors.initials: L
authors.lastname: Kong
authors.affiliation: The Key Laboratory for Biology of Insect Pests and Plant Disease, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China; Key Laboratory of Pests Comprehensive Governance for Tropical Crops, Environment and Plant protection Institute, Chinese Academy of Tropical Agricultural Science, Danzhou, China.
authors.forename: Haibo
authors.initials: H
authors.lastname: Long
authors.affiliation: The Key Laboratory for Biology of Insect Pests and Plant Disease, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Peng
authors.affiliation: Department of Plant Pathology, China Agricultural University, Beijing, China.
authors.forename: Heng
authors.initials: H
authors.lastname: Jian
mesh_terms: D000818:Animals; D005260:Female; D020869:Gene Expression Profiling; D015723:Gene Library; D063990:Gene Ontology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015203:Reproducibility of Results; D013025:Glycine max / Q000469:parasitology; D013045:Species Specificity; D014431:Tylenchoidea / Q000235:genetics* / Q000254:growth & development* / Q000502:physiology
chemical_list: D012333:RNA, Messenger