title: Quantitative measurement of influenza virus replication using consecutive bronchoalveolar lavage in the lower respiratory tract of a ferret model.
abstract: The ferret is an established animal model of influenza virus infection. Although viral replication in the upper respiratory tract is usually measured with consecutively collected nasal washes, daily evaluation of viral replication in the lung is limited because a large numbers of ferrets need to be sacrificed at consecutive time points. To overcome this limitation, we performed a virus quantification assay using bronchoalveolar lavage (BAL) fluid. This non-invasive BAL technique allows consecutive quantification of virus replication in the lungs of living ferrets. Our method can be used for the longitudinal evaluation of virus tropism in the lower respiratory tract.
authors.affiliation: Avian Disease Laboratory, College of Veterinary Medicine, Konkuk University, Seoul 143-701, Korea.
authors.forename: Dong-Hun
authors.initials: DH
authors.lastname: Lee
authors.forename: Jong-In
authors.initials: JI
authors.lastname: Kim
authors.forename: Jae-Won
authors.initials: JW
authors.lastname: Lee
authors.forename: Wook-Hun
authors.initials: WH
authors.lastname: Chung
authors.forename: Jae-Keun
authors.initials: JK
authors.lastname: Park
authors.forename: Yu-Na
authors.initials: YN
authors.lastname: Lee
authors.forename: Jin Soo
authors.initials: JS
authors.lastname: Han
authors.forename: Hwi-Yool
authors.initials: HY
authors.lastname: Kim
authors.forename: Sang-Won
authors.initials: SW
authors.lastname: Lee
authors.forename: Chang-Seon
authors.initials: CS
authors.lastname: Song
mesh_terms: D000818:Animals; D018893:Bronchoalveolar Lavage / Q000662:veterinary*; D004195:Disease Models, Animal; D005260:Female; D005289:Ferrets / Q000821:virology*; D053122:Influenza A Virus, H3N2 Subtype / Q000502:physiology*; D009976:Orthomyxoviridae Infections / Q000662:veterinary* / Q000821:virology; D012137:Respiratory System / Q000821:virology*; D014779:Virus Replication / Q000502:physiology*
keywords: bronchoalveolar lavage; ferret; infection; influenza