title: Potentially probiotic Lactobacillus strains from traditional Kurdish cheese.
abstract: In this study, the probiotic potential of Lactobacillus strains isolated from traditional Kurdish cheese was investigated. The Lactobacillus strains were examined for resistance to gastric acidity and bile toxicity, antimicrobial activities, autoaggregation, coaggregation, hydrophobicity, adhesion to Caco-2 cells, and antibiotic susceptibility. The results showed that all strains tested tolerate acid gastric conditions (pH 2.0 and 3.0), and all of them were bile resistant (at 0.3 and 1% concentration). Although no antibacterial activity was detected in vitro assay for the treated (neutralized to pH 6.5 and treated with catalase) cell-free culture supernatant (CFCS) of strains, untreated CFCS showed strong antagonistic activity against two known pathogens bacteria. All strains exhibited a strong autoaggregating phenotype and manifested a high degree of coaggregation with pathogens. On the other hand, majority of studied strains were found sensitive to different antibiotics, such as ampicillin, penicillin, ciprofloxacin, chloramphenicol, erythromycin, rifampicin, and tetracycline, and were resistant to vancomycin and streptomycin. Finally, isolated strains showed good hydrophobicity and adherence to Caco-2 cell line, so they could be exploited for food manufacture.
authors.affiliation: Department of Food Science and Technology, College of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran, hasshemii@yahoo.com.
authors.forename: Seyed Mohammad Bagher
authors.initials: SM
authors.lastname: Hashemi
authors.forename: Fakhri
authors.initials: F
authors.lastname: Shahidi
authors.forename: Seyed Ali
authors.initials: SA
authors.lastname: Mortazavi
authors.forename: Elnaz
authors.initials: E
authors.lastname: Milani
authors.forename: Zarrin
authors.initials: Z
authors.lastname: Eshaghi
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001422:Bacterial Adhesion; D018938:Caco-2 Cells; D002611:Cheese / Q000382:microbiology*; D024901:Drug Resistance, Multiple, Bacterial; D005516:Food Microbiology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D007778:Lactobacillus / Q000302:isolation & purification* / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D019936:Probiotics
chemical_list: D000900:Anti-Bacterial Agents