title: [The shRNA-mediated downregulation of protein kinase D-2 enhanced chemosensitivity of Tca8113].
abstract: OBJECTIVE
  To explore the effect of silencing protein kinase D (PKD)-2 on Tca8113 cell proliferation, programmed cell death, and chemosensitivity.
  
  
  METHODS
  The stable cell lines of pkd-2 gene silencing and empty vector plasmid group were established. The proliferation and 50% inhibitory concentration (IC50) of shRNA-mediated Tca8113 chemotherapy drugs were detected through methyl thiazolyl tetrazolium assay (MTT). The programmed cell death rate and sensitivity to Tca8113 chemotherapy drugs before or after pkd-2 gene silencing were measured through flow cytometry. P-glycoprotein (P-gp) expression of pkd-2 silencing cells was identified by immunohistochemical methods.
  
  
  RESULTS
  Stable cell lines of pkd-2 gene silencing were established. Compared with parental cells, the proliferation of shRNA-mediated Tca8113 was not significantly different, but its IC50 was lower. Meanwhile, cell programmed death rate and sensitivity to chemotherapeutic drugs of shRNA-mediated Tca8113 significantly increased. Compared with wild group Tca8113, a significant decrease in P-gp expression was induced by chemotherapy drugs with shRNA-pkd-2 gene silencing.
  
  
  CONCLUSIONS
  The pkd-2 gene of shRNA interference silencing Tca8113 promotes programmed cell death of Tca8113, reduces the IC50 of the chemotherapy drugs, and significantly improves the sensitivity of Tca8113 to chemotherapeutic drugs while reducing the expression of P-gp.
authors.forename: Lina
authors.initials: L
authors.lastname: Dai
authors.forename: Ping
authors.initials: P
authors.lastname: Zhang
authors.forename: Qin
authors.initials: Q
authors.lastname: Su
mesh_terms: D000970:Antineoplastic Agents; D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D020868:Gene Silencing; D005822:Genetic Vectors; D006801:Humans; D011493:Protein Kinase C; D034741:RNA, Small Interfering; D014162:Transfection
chemical_list: D000970:Antineoplastic Agents; D034741:RNA, Small Interfering; C089212:protein kinase D; D011493:Protein Kinase C