Sex differences in constitutive autophagy.
Sex bias has been described nowadays in biomedical research on animal models, although sexual dimorphism has been confirmed widely under pathological and physiological conditions. The main objective of our work was to study the sex differences in constitutive autophagy in spinal cord and skeletal muscle tissue from wild type mice. To examine the influence of sex on autophagy, mRNA and proteins were extracted from male and female mice tissues. The expressions of microtubule-associated protein 1 light chain 3 (LC3) and sequestosome 1 (p62), markers to monitor autophagy, were analyzed at 40, 60, 90, and 120 days of age. We found significant sex differences in the expression of LC3 and p62 in both tissues at these ages. The results indicated that sex and tissue specific differences exist in constitutive autophagy. These data underlined the need to include both sexes in the experimental groups to minimize any sex bias.
LAGENBIO-I3A, Veterinary School, University of Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain.
Sara
S
Oliván
LAGENBIO-I3A, Veterinary School, University of Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain.
Ana Cristina
AC
Calvo
LAGENBIO-I3A, Veterinary School, University of Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain.
Raquel
R
Manzano
LAGENBIO-I3A, Veterinary School, University of Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain.
Pilar
P
Zaragoza
LAGENBIO-I3A, Veterinary School, University of Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain.
Rosario
0000-0001-5687-6704
R
Osta
D000818:Animals; D001343:Autophagy / Q000502:physiology*; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D009928:Organ Specificity / Q000502:physiology; D012727:Sex Characteristics