Mesenteric lymph drainage alleviates acute kidney injury induced by hemorrhagic shock without resuscitation.
This study aimed to investigate the effect of mesenteric lymph drainage on the acute kidney injury induced by hemorrhagic shock without resuscitation. Eighteen male Wistar rats were randomly divided into sham, shock, and drainage groups. The hemorrhagic shock model (40 mmHg, 3 h) was established in shock and drainage groups; mesenteric lymph drainage was performed from 1 h to 3 h of hypotension in the drainage group. The results showed that renal tissue damage occurred; the levels of urea, creatinine, and trypsin in the plasma as well as intercellular adhesion molecule-1 (ICAM-1), receptor of advanced glycation end-products (RAGE), tumor necrosis factor-α (TNF-α), malondialdehyde (MDA), lactic acid (LA), and 2,3-DPG in the renal tissue were increased in the shock group after 3 h of hypotension. Mesenteric lymph drainage lessened the following: renal tissue damage; urea and trypsin concentrations in the plasma; ICAM-1, RAGE, TNF-α, MDA, and LA levels in the renal tissue. By contrast, mesenteric lymph drainage increased the 2,3-DPG level in the renal tissue. These findings indicated that mesenteric lymph drainage could relieve kidney injury caused by sustained hypotension, and its mechanisms involve the decrease in trypsin activity, suppression of inflammation, alleviation of free radical injury, and improvement of energy metabolism.
Institute of Microcirculation, Hebei North University, Diamond South Road 11, Zhangjiakou, Hebei 075000, China.
Zi-Gang
ZG
Zhao
Institute of Microcirculation, Hebei North University, Diamond South Road 11, Zhangjiakou, Hebei 075000, China.
Hong-Xia
HX
Zhu
Institute of Microcirculation, Hebei North University, Diamond South Road 11, Zhangjiakou, Hebei 075000, China.
Li-Min
LM
Zhang
Institute of Microcirculation, Hebei North University, Diamond South Road 11, Zhangjiakou, Hebei 075000, China.
Yu-Ping
YP
Zhang
Institute of Microcirculation, Hebei North University, Diamond South Road 11, Zhangjiakou, Hebei 075000, China.
Chun-Yu
CY
Niu
D019794:2,3-Diphosphoglycerate / Q000378:metabolism; D058186:Acute Kidney Injury / Q000209:etiology* / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology / Q000601:surgery*; D000818:Animals; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D004195:Disease Models, Animal; D004322:Drainage / Q000379:methods*; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D007677:Kidney Function Tests; D019344:Lactic Acid / Q000378:metabolism; D042601:Lymphatic Vessels / Q000601:surgery*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051381:Rats; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic / Q000378:metabolism; D012151:Resuscitation; D012771:Shock, Hemorrhagic / Q000150:complications*; D013152:Splanchnic Circulation; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D015415:Biomarkers; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic; D014409:Tumor Necrosis Factor-alpha; D018799:Intercellular Adhesion Molecule-1; D019794:2,3-Diphosphoglycerate; D019344:Lactic Acid; D008315:Malondialdehyde