Increased expression of three types of transient receptor potential channels (TRPA1, TRPV4 and TRPV3) in burn scars with post-burn pruritus.
Post-burn pruritus is a common distressing consequence of burn wounds. Empirical treatment often fails to have a satisfactory outcome on post-burn pruritus, as the mechanism of post-burn pruritus has not been fully elucidated. The aim of this study was to evaluate the manifestation of transient receptor potential (TRP) channels in post-burn pruritus. Fifty-one burn patients with (n=33) or without (n=18) pruritus were investigated, including skin biopsies. Not unexpectedly, the scarred body area was larger in the former group. In immunohistochemistry, TPRV3 was significantly elevated in the epidermis of burn scars with pruritus. Furthermore, real time- PCR showed that mRNA of TRPA1 and TRPV4 was increased in itching burn scars. Staining for substance P and CGRP did not differ between the 2 grouped, but the former neuropeptide was increased in burn scars. These results may help determine a specific therapeutic approach for post-burn pruritus.
Department of Dermatology, College of Medicine, Hallym University, Kangnam Sacred Heart Hospital, 948-1, Daerim 1-dong, Yeungdeungpo-gu, Seoul, Korea.
Yoon Seok
YS
Yang
Soo Ick
SI
Cho
Min Gyu
MG
Choi
Young Hee
YH
Choi
In Suk
IS
Kwak
Chun Wook
CW
Park
Hye One
HO
Kim
D000293:Adolescent; D000328:Adult; D001706:Biopsy; D002056:Burns / Q000150:complications*; D015740:Calcitonin Gene-Related Peptide / Q000032:analysis; D015220:Calcium Channels / Q000032:analysis* / Q000235:genetics; D002648:Child; D002921:Cicatrix / Q000175:diagnosis / Q000209:etiology / Q000235:genetics / Q000378:metabolism*; D004817:Epidermis / Q000737:chemistry* / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009419:Nerve Tissue Proteins / Q000032:analysis* / Q000235:genetics; D011537:Pruritus / Q000175:diagnosis / Q000209:etiology / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D060888:Real-Time Polymerase Chain Reaction; D013373:Substance P / Q000032:analysis; D000074025:TRPA1 Cation Channel; D050916:TRPV Cation Channels / Q000032:analysis* / Q000235:genetics; D050051:Transient Receptor Potential Channels / Q000032:analysis* / Q000235:genetics; D055815:Young Adult
D015220:Calcium Channels; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D000074025:TRPA1 Cation Channel; C473350:TRPA1 protein, human; D050916:TRPV Cation Channels; C459445:TRPV3 protein, human; C416986:TRPV4 protein, human; D050051:Transient Receptor Potential Channels; D013373:Substance P; D015740:Calcitonin Gene-Related Peptide