Simple, low-cost molecular assays for TR34/L98H mutations in the cyp51A gene for rapid detection of triazole-resistant Aspergillus fumigatus isolates.
Simple, low-cost PCR/PCR-restriction fragment length polymorphism (RFLP) assays targeting cyp51A promoter and codon 98 regions were developed for the detection of triazole-resistant Aspergillus fumigatus strains carrying TR34/L98H mutations. The assays were evaluated using 40 itraconazole-susceptible isolates and 35 itraconazole-resistant isolates. The prevalence of TR34/L98H mutations in clinical/environmental A. fumigatus isolates may now be determined easily from resource-poor settings.
Department of Microbiology, Faculty of Medicine, Kuwait University, Kuwait City, Kuwait.
Suhail
S
Ahmad
Department of Microbiology, Faculty of Medicine, Kuwait University, Kuwait City, Kuwait zkhan@hsc.edu.kw.
Ziauddin
Z
Khan
Department of Medical Microbiology and Infectious Diseases, Canisius Wilhelmina Hospital, Nijmegen, The Netherlands.
Ferry
F
Hagen
Department of Medical Microbiology and Infectious Diseases, Canisius Wilhelmina Hospital, Nijmegen, The Netherlands Department of Medical Microbiology, Radboud University Medical Center, Nijmegen, The Netherlands.
Jacques F
JF
Meis
D000935:Antifungal Agents / Q000494:pharmacology*; D001232:Aspergillus fumigatus / Q000187:drug effects* / Q000201:enzymology* / Q000235:genetics; D003365:Costs and Cost Analysis; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics*; D060005:Genotyping Techniques / Q000191:economics / Q000379:methods*; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D020125:Mutation, Missense; D016133:Polymerase Chain Reaction / Q000191:economics / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D014230:Triazoles / Q000494:pharmacology*
D000935:Antifungal Agents; D005656:Fungal Proteins; D014230:Triazoles; D003577:Cytochrome P-450 Enzyme System; C465526:cytochrome P-450 CYP51A, Aspergillus