Viable bacterial cell patterning using a pulsed jet electrospray system.
In the present study, drop-on-demand two-dimensional patterning of unstained and stained bacterial cells on untreated clean wafers was newly conducted using an electrospray pulsed jet. We produced various spotted patterns of the cells on a silicon wafer by varying the experimental conditions, such as the frequency, flow rate, and translational speed of the electrospray system in a two-dimensional manner. Specifically, the electrospray's pulsed jet of cell solutions produced alphabetical patterns consisting of spots with a diameter of approximately 10 μm, each of which contained a single or a small number of viable bacteria. We tested the viability of the patterned cells using two visualization methods. This patterning technique is newly tested here and it has the potential to be applied in a variety of cell biology experiments.
Aerosol and bioengineering laboratory, Department of Mechanical Engineering, Konkuk University, Seoul 143-701, Republic of Korea.
Eui-seok
ES
Chong
Gi Byung
GB
Hwang
Kyoungtae
K
Kim
Im-Soon
IS
Lee
Song Hee
SH
Han
Hyung Joo
HJ
Kim
Heehoon
H
Jung
Sung-Jin
SJ
Kim
Hyo Il
HI
Jung
Byung Uk
BU
Lee
D001419:Bacteria / Q000166:cytology*; D050296:Microbial Viability; D020411:Oligonucleotide Array Sequence Analysis