Electrospun soy protein nanofiber scaffolds for tissue regeneration.
Electrospun fibers with an average fiber diameter in the nanometer range were prepared from soy protein isolate to develop scaffolds for tissue engineering applications. Poly(ethylene oxide) was added to facilitate fiber formation. The influence of processing parameters such as applied voltage, soy protein isolate and poly(ethylene oxide) concentrations, and poly(ethylene oxide) molecular weight on electrospun fiber morphology was investigated. Resulting soy protein isolate/poly(ethylene oxide) mats were carbodiimide crosslinked to increase construct robustness. Mechanical properties and in vitro biocompatibility of crosslinked electrospun scaffolds were evaluated. Soy protein isolate/poly(ethylene oxide) fiber diameters ranged between 50 and 270 nm depending on both electrospinning and solution parameters. The Young's modulus for 7% soy protein isolate/3% poly(ethylene oxide) and 12% soy protein isolate/3% poly(ethylene oxide) electrospun scaffolds were 75 and 252 kPa, respectively. Human mesenchymal stem cell studies showed successful cell adhesion and proliferation on the soy protein isolate/poly(ethylene oxide) fibers. The structural and biological properties of these soy protein isolate electrospun scaffolds suggest their potential applications in tissue engineering.
Department of Materials Science and Engineering, Northwestern University, Evanston, United States kramji7@gmail.com.
Karpagavalli
K
Ramji
Department of Materials Science and Engineering, Northwestern University, Evanston, United States Institute for BioNanotechnology in Medicine and Department of Surgery, Northwestern University, Chicago, United States.
Ramille N
RN
Shah
D002478:Cells, Cultured; D055664:Electrochemical Techniques / Q000379:methods*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D057139:Nanofibers; D012038:Regeneration; D030262:Soybean Proteins / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013500:Surface Tension; D054457:Tissue Scaffolds; D014783:Viscosity
D030262:Soybean Proteins
Soy protein isolate; electrospinning; nanofibers; scaffolds; tissue regeneration