Vasonatrin peptide stimulates both of the natriuretic peptide receptors, NPRA and NPRB.
Vasonatrin peptide (VNP) is an active cardiovascular factor and a novel synthetic natriuretic peptide with unknown natriuretic peptide receptor (NPR) binding properties. We set out to design binding models of NPRA/VNP and NPRB/VNP, and then assessed their recognition and binding affinities using molecular dynamics. Molecular dynamics analysis indicated decreases in the values of Van der Waals, electrostatic energy and potential energy of NPRB/VNP compared to NPRA/VNP. There was a 25% increase in H-bond formation between VNP and NPRB. The cGMP stimulated by VNP in NPRB-transfected HEK-293 cells was 11-fold higher than that of NPRA. We therefore demonstrated that VNP binds with both NPRA and NPRB, but with a preference for NPRB.
School of Pharmaceutical Sciences, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, PR China.
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YS
Jiang
School of Pharmaceutical Sciences, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, PR China.
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JY
Lei
School of Pharmaceutical Sciences, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, PR China.
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Chen
School of Pharmaceutical Sciences, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, PR China; Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences and Shanghai Jiao-Tong University School of Medicine, 320 Yue-Yang Road, Shanghai 200031, PR China. Electronic address: jinjian31@126.com.
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D009320:Atrial Natriuretic Factor / Q000737:chemistry / Q000378:metabolism*; D001665:Binding Sites; D057809:HEK293 Cells; D006801:Humans; D062105:Molecular Docking Simulation; D056004:Molecular Dynamics Simulation; D017461:Receptors, Atrial Natriuretic Factor / Q000737:chemistry / Q000378:metabolism*; D000099242:Atrial Natriuretic Factor Receptor B
D009320:Atrial Natriuretic Factor; D017461:Receptors, Atrial Natriuretic Factor; D000099242:Atrial Natriuretic Factor Receptor B; C068986:ventricular natriuretic peptide, eel; C091715:atrial natriuretic factor receptor A
Binding affinity; Molecular dynamics; Natriuretic peptide; Natriuretic peptide receptor; Vasonatrin peptide