Fabrication of multiwalled carbon nanotubes-magnetite nanocomposite as an effective ultra-sensing platform for the early screening of nasopharyngeal carcinoma by luminescence immunoassay.
The hybrid nanocomposite that consists of multiwalled carbon nanotubes (MWCNTs) and magnetite (Fe₃O4) was fabricated by chemical co-precipitation method. Briefly, CNTs were oxidized with acids to form carboxylic group and then co-precipitated with Fe₃O4 to form CNT-Fe₃O4 nanocomposites. The nanocomposites were characterized by SEM, HRTEM, XRD, FTIR X-ray photoelectron spectrometry (XPS) and SQUID. The XRD results indicated the high crystallinity of Fe₃O₄ nanoparticles with spinel structure and the transmission electron microscope images depicted the intercalated iron oxide magnetic particles on the surface of CNTs. The MWCNTs-Fe₃O₄ was applied as a sensing interface to perform luminescence enzyme immunoassays. Firstly, EBNA-1 antigen was immobilized onto the carboxyl group functionalized MWCNTs-Fe₃O₄, followed by binding with anti-EBNA-1 IgA antibodies. The diluted secondary antibodies (anti-human IgA-HRP) were then added to the CNTs/Fe₃O₄-PEG-EBNA-1-anti-EBV IgA ab complex and act as a catalyst to produce a visible light upon reaction with the substrate luminol. The formed RLU is proportional to the amount of IgA anti-EBV antiobodies on the MWCNTs. The detection limit of proposed CNTs/Fe₃O₄ based luminescence enzyme immunoassay was in the order of 0.00128 EU/mL (1:100,000 fold dilution) for the detection of anti-EBV IgA antibodies, whereas the commercial ELISA and magnetic beads' assay was accounted for up to the dilution fold of 1000 (i.e., 0.128 EU/mL). The initial findings showed that CNTs/Fe₃O₄ nanocomposites have a great potential in luminescent enzyme immunoassays and could be used as a sensing platform for the early screening of nasopharyngeal carcinoma.
Institute of Biomedical Engineering, National Taiwan University, Taipei 100, Taiwan.
Chia-Ching
CC
Liu
Institute of Biomedical Engineering and Nanomedicine, National Health Research Institutes, Miaoli 350, Taiwan.
S
S
Sadhasivam
Department of Biotechnology, Sree Sastha Institute of Engineering and Technology, Chennai, India.
S
S
Savitha
Institute of Biomedical Engineering, National Taiwan University, Taipei 100, Taiwan; Institute of Biomedical Engineering and Nanomedicine, National Health Research Institutes, Miaoli 350, Taiwan. Electronic address: double@ntu.edu.tw.
Feng-Huei
FH
Lin
D002277:Carcinoma; D055088:Early Detection of Cancer / Q000295:instrumentation / Q000379:methods; D006801:Humans; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D053761:Nanocomposites / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000097:blood* / Q000175:diagnosis
D058185:Magnetite Nanoparticles; D037742:Nanotubes, Carbon
Fe(3)O(4); Immunosensors; Luminescent assay; Multiwalled CNTs; Nanocomposites; Nasopharyngeal carcinoma