Synthetic antibody technologies.
Synthetic antibody technologies enable the rapid production of affinity reagents through in vitro selections. The production of synthetic antibodies relies on sophisticated design strategies to produce combinatorial diversity libraries that encode antibody populations optimized for molecular recognition. The technology takes advantage of display technologies that enable amplification, selection and manipulation of antibodies in vitro. The rapid yet highly controlled nature of these methods has opened new avenues in basic and clinical research. Here we review recent advances in structural biology facilitated by synthetic antibodies, as well as advances in library designs and selection methods.
Banting and Best Department of Medical Research and Department of Molecular Genetics, University of Toronto, Donnelly CCBR, 160 College Street, Toronto, Ontario M5S 3E1, Canada.
Jarrett J
JJ
Adams
Banting and Best Department of Medical Research and Department of Molecular Genetics, University of Toronto, Donnelly CCBR, 160 College Street, Toronto, Ontario M5S 3E1, Canada. Electronic address: sachdev.sidhu@utoronto.ca.
Sachdev S
SS
Sidhu
D000345:Affinity Labels / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D000906:Antibodies / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D003460:Crystallization / Q000379:methods; D015723:Gene Library; D006801:Humans; D008958:Models, Molecular; D019151:Peptide Library
D000345:Affinity Labels; D000906:Antibodies; D019151:Peptide Library