Ferutinin, an apoptosis inducing terpenoid from Ferula ovina.
A current hurdle in cancer management is the intrinsic or acquired resistance of cancer cells to chemical agents that restricts the efficacy of therapeutic strategies. Accordingly, there is an increasing desire to discover new natural compounds with selective toxicity to combat malignancies. In present study, the cytotoxic and apoptosis- inducing activities of ferutinin, a terpenoid derivative from Ferula ovina, were investigated on human breast (MCF7) and bladder (TCC) cancer cells as well as normal fibroblasts (HFF3).The toxicity and DNA damage inducing effects of ferutinin were studied by MTT and comet assays, DAPI and PI staining and DNA laddering. The IC50 values of ferutinin were identified and compared with routine prescribed drugs, doxorubicin and vincristine, by MTT test. Alkaline comet assay and DAPI staining revealed DNA damage due to ferutinin, which was significantly (p<0.001) higher in MCF7 and TCC than HFF3 cells. Apoptosis induction was evidenced by PI staining and DNA laddering. Our results suggest that ferutinin could be considered as an effective anticancer agent for future in vivo and clinical experiments.
Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran E-mail : Matin@um.ac.ir.
Maryam Moghaddam
MM
Matin
Hossein
H
Nakhaeizadeh
Ahamd Reza
AR
Bahrami
Mehrdad
M
Iranshahi
Nahid
N
Arghiani
Fatemeh Behnam
FB
Rassouli
D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001565:Benzoates / Q000737:chemistry / Q000494:pharmacology*; D001643:Bridged Bicyclo Compounds / Q000737:chemistry / Q000494:pharmacology; D002460:Cell Line; D045744:Cell Line, Tumor; D003508:Cycloheptanes / Q000737:chemistry / Q000494:pharmacology*; D004249:DNA Damage / Q000187:drug effects; D004317:Doxorubicin / Q000494:pharmacology; D005309:Ferula / Q000737:chemistry*; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D061986:MCF-7 Cells; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D012717:Sesquiterpenes / Q000737:chemistry / Q000494:pharmacology*; D013729:Terpenes / Q000737:chemistry / Q000494:pharmacology*; D014750:Vincristine / Q000494:pharmacology
D000970:Antineoplastic Agents; D001565:Benzoates; D001643:Bridged Bicyclo Compounds; D003508:Cycloheptanes; D010936:Plant Extracts; D012717:Sesquiterpenes; D013729:Terpenes; C111068:4-oxy-6-(4-oxybezoyloxy)dauc-8,9-en; D014750:Vincristine; D004317:Doxorubicin