Facile and effective pretreatment using stop and go extraction tips for LC-MS/MS analysis of trace amounts of DNA adducts.
The preparation and application of a stop and go extraction tip (StageTip) used for pre-purification of sample solutions for LC-MS/MS analysis of DNA adducts was simplified and improved to increase throughput while maintaining high adduct selectivity. It was demonstrated that the StageTip composed of two sheets of a poly(styrene-divinylbenzene) copolymer disk could be easily prepared and proved useful for selective extraction of trace amounts of DNA adducts from a sample solution containing a great quantity of normal deoxynucleosides.
Gifu Pharmaceutical University.
Hiroya
H
Murakami
Rieko
R
Kawamura
Takayoshi
T
Sakakibara
Yukihiro
Y
Esaka
Yasushi
Y
Ishihama
Bunji
B
Uno
D053000:Analytic Sample Preparation Methods / Q000295:instrumentation / Q000379:methods*; D005591:Chemical Fractionation; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D018736:DNA Adducts / Q000032:analysis*; D003853:Deoxyribonucleosides / Q000737:chemistry; D057166:High-Throughput Screening Assays; D011137:Polystyrenes / Q000737:chemistry; D012680:Sensitivity and Specificity; D012996:Solutions; D053719:Tandem Mass Spectrometry / Q000295:instrumentation / Q000379:methods*
D018736:DNA Adducts; D003853:Deoxyribonucleosides; D011137:Polystyrenes; D012996:Solutions; C003771:Amberlite XAD-2 resin