On the effects of intercalators in DNA condensation: a force spectroscopy and gel electrophoresis study.
In this work we have characterized the effects of the intercalator ethidium bromide (EtBr) on the DNA condensation process by using force spectroscopy and gel electrophoresis. We have tested two condensing agents: spermine (spm(4+)), a tetravalent cationic amine which promotes cation-induced DNA condensation, and poly(ethylene glycol) (PEG), a neutral polymer which promotes DNA ψ-condensation. Two different types of experiments were performed. In the first type, bare DNA molecules disperse in solution are first treated with EtBr for intercalation, and then the condensing agent is added to the sample with the purpose of verifying the effects of the intercalator in hindering DNA condensation. In the second experiment type, the bare DNA molecules are first condensed, and then the intercalator is added to the sample in order to verify its influence on the previously condensed DNA. The results obtained with the two different experimental techniques used agree very well, indicating that previously intercalated EtBr can hinder both cation-induced and ψ-condensation, being more efficient in the first case. On the other hand, EtBr has little effect on the previously formed cation-induced condensates, but is efficient in unfolding the ψ-condensates.
Laboratório de Física Biológica, Departamento de Física, Universidade Federal de Viçosa , Viçosa, Minas Gerais, 36570-000, Brazil.
M S
MS
Rocha
A G
AG
Cavalcante
R
R
Silva
E B
EB
Ramos
D004247:DNA / Q000737:chemistry*; D004586:Electrophoresis; D004996:Ethidium / Q000494:pharmacology*; D007364:Intercalating Agents / Q000494:pharmacology*; D009690:Nucleic Acid Conformation / Q000187:drug effects*; D011092:Polyethylene Glycols / Q000494:pharmacology*; D013057:Spectrum Analysis; D013096:Spermine / Q000494:pharmacology*
D007364:Intercalating Agents; D013096:Spermine; D011092:Polyethylene Glycols; D004247:DNA; D004996:Ethidium