Water-soluble platinum phthalocyanines as potential antitumor agents.
Breast cancer represents the second cause of death in the European female population. The lack of specific therapies together with its high invasive potential are the major problems associated to such a tumor. In the last three decades platinum-based drugs have been considered essential constituents of many therapeutic strategies, even though with side effects and frequent generation of drug resistance. These drugs have been the guide for the research, in last years, of novel platinum and ruthenium based compounds, able to overcome these limitations. In this work, ruthenium and platinum based phthalocyanines were synthesized through conventional techniques and their antiproliferative and/or cytotoxic actions were tested. Normal mammary gland (MCF10A) and several models of mammarian carcinoma at different degrees of invasiveness (BT474, MCF-7 and MDA-MB-231) were used. Cells were treated with different concentrations (5-100 μM) of the above reported compounds, to evaluate toxic concentration and to underline possible dose-response effects. The study included growth curves made by trypan blue exclusion test and scratch assay to study cellular motility and its possible negative modulation by phthalocyanine. Moreover, we investigated cell cycle and apoptosis through flow cytometry and AMNIS Image Stream cytometer. Among all the tested drugs, tetrasulfonated phthalocyanine of platinum resulted to be the molecule with the best cytostatic action on neoplastic cell lines at the concentration of 30 μM. Interestingly, platinum tetrasulfophtalocyanine, at low doses, had no antiproliferative effects on normal cells. Therefore, such platinum complex, appears to be a promising drug for mammarian carcinoma treatment.
Department of Medicine and Aging Science, School of Medicine and Health Sciences, University "G. d'Annunzio" of Chieti-Pescara, Via dei Vestini, 31, 66013, Chieti Scalo, Italy.
Giuseppina
G
Bologna
Paola
P
Lanuti
Primiano
P
D'Ambrosio
Lucia
L
Tonucci
Laura
L
Pierdomenico
Carlo
C
D'Emilio
Nicola
N
Celli
Marco
M
Marchisio
Nicola
N
d'Alessandro
Eugenio
E
Santavenere
Mario
M
Bressan
Sebastiano
S
Miscia
D000970:Antineoplastic Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000188:drug therapy*; D002453:Cell Cycle / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D005260:Female; D006801:Humans; D007211:Indoles / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D061986:MCF-7 Cells; D009944:Organoplatinum Compounds / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D012995:Solubility; D014867:Water / Q000737:chemistry*
D000970:Antineoplastic Agents; D007211:Indoles; D009944:Organoplatinum Compounds; C000594169:platinum tetrasulfophtalocyanine; D014867:Water