Distinct Rayleigh scattering from hot spot mutant p53 proteins reveals cancer cells.
The scattering of light redirects and resonances when an electromagnetic wave interacts with electrons orbits in the hot spot core protein and oscillated electron of the gold nanoparticles (AuNP). This report demonstrates convincingly that resonant Rayleigh scattering generated from hot spot mutant p53 proteins is correspondence to cancer cells. Hot spot mutants have unique local electron density changes that affect specificity of DNA binding affinity compared with wild types. Rayleigh scattering changes introduced by hot-spot mutations were monitored by localized surface plasmon resonance (LSPR) shift changes. The LSPR λmax shift for hot-spot mutants ranged from 1.7 to 4.2 nm for mouse samples and from 0.64 nm to 2.66 nm for human samples, compared to 9.6 nm and 15 nm for wild type and mouse and human proteins, respectively with a detection sensitivity of p53 concentration at 17.9 nM. It is interesting that hot-spot mutants, which affect only interaction with DNA, launches affinitive changes as considerable as wild types. These changes propose that hot-spot mutants p53 proteins can be easily detected by local electron density alterations that disturbs the specificity of DNA binding of p53 core domain on the surface of the DNA probed-nanoplasmonic sensor.
Department of Chemical and Biological Engineering, Korea University, Seoul, 136-701, Korea.
Ho Joon
HJ
Jun
Anh H
AH
Nguyen
Yeul Hong
YH
Kim
Kyong Hwa
KH
Park
Doyoun
D
Kim
Kyeong Kyu
KK
Kim
Sang Jun
SJ
Sim
D000818:Animals; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000235:genetics; D045744:Cell Line, Tumor; D004273:DNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D005260:Female; D016158:Genes, p53; D006046:Gold; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism; D053768:Metal Nanoparticles; D051379:Mice; D050505:Mutant Proteins / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism; D009369:Neoplasms / Q000235:genetics / Q000378:metabolism*; D009687:Nuclear Proteins / Q000235:genetics; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D012542:Scattering, Radiation; D020349:Surface Plasmon Resonance / Q000379:methods; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism*; D000097642:GADD45 Proteins
D018797:Cell Cycle Proteins; D004273:DNA, Neoplasm; D006046:Gold; D050505:Mutant Proteins; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D016159:Tumor Suppressor Protein p53; C439070:GADD45A protein, human; D000097642:GADD45 Proteins
gold nanoparticles; hot spot mutants; localized surface plasmon resonance (LSPR); p53 protein mutation; rayleigh scattering