Cardiomyocyte-specific expression of CRNK, the C-terminal domain of PYK2, maintains ventricular function and slows ventricular remodeling in a mouse model of dilated cardiomyopathy.
Up-regulation and activation of PYK2, a member of the FAK family of protein tyrosine kinases, is involved in the pathogenesis of left ventricular (LV) remodeling and heart failure (HF). PYK2 activation can be prevented by CRNK, the C-terminal domain of PYK2. We previously demonstrated that adenoviral-mediated CRNK gene transfer improved survival and LV function, and slowed LV remodeling in a rat model of coronary artery ligation-induced HF. We now interrogate whether cardiomyocyte-specific, transgenic CRNK expression prevents LV remodeling and HF in a mouse model of dilated cardiomyopathy (DCM) caused by constitutively active Protein Kinase Cε (caPKCε). Transgenic (TG; FVB/N background) mice were engineered to express rat CRNK under control of the α-myosin heavy chain promoter, and crossed with FVB/N mice with cardiomyocyte-specific expression of caPKCε to create double TG mice. LV structure, function, and gene expression were evaluated in all 4 groups (nonTG FVB/N; caPKCε(+/-); CRNK(+/-); and caPKCε×CRNK (PXC) double TG mice) at 1, 3, 6, 9 and 12mo of age. CRNK expression followed a Mendelian distribution, and CRNK mice developed and survived normally through 12mo. Cardiac structure, function and selected gene expression of CRNK mice were similar to nonTG littermates. CRNK had no effect on caPKCε expression and vice versa. PYK2 was up-regulated ~6-fold in caPKCε mice, who developed a non-hypertrophic, progressive DCM with reduced systolic (Contractility Index=151±5 vs. 90±4s(-1)) and diastolic (Tau=7.5±0.5 vs. 14.7±1.3ms) function, and LV dilatation (LV Remodeling Index (LVRI)=4.2±0.1 vs. 6.0±0.3 for FVB/N vs. caPKCε mice, respectively; P<0.05 for each at 12mo). In double TG PXC mice, CRNK expression significantly prolonged survival, improved contractile function (Contractile Index=115±8s(-1); Tau=9.5±1.0ms), and reduced LV remodeling (LVRI=4.9±0.1). Cardiomyocyte-specific expression of CRNK improves contractile function and slows LV remodeling in a mouse model of DCM.
Department of Medicine, Loyola University Chicago Stritch School of Medicine, Maywood, IL 60153, USA.
Yevgeniya E
YE
Koshman
Department of Cell and Molecular Physiology, Loyola University Chicago Stritch School of Medicine, Maywood, IL 60153, USA.
Miensheng
M
Chu
Department of Medicine, Loyola University Chicago Stritch School of Medicine, Maywood, IL 60153, USA.
Taehoon
T
Kim
Department of Cell and Molecular Physiology, Loyola University Chicago Stritch School of Medicine, Maywood, IL 60153, USA.
Olivia
O
Kalmanson
Department of Physiology and Biophysics, University of Illinois - Chicago, Chicago, IL 60612, USA.
Mariam
M
Farjah
Department of Cell and Molecular Physiology, Loyola University Chicago Stritch School of Medicine, Maywood, IL 60153, USA.
Mohit
M
Kumar
Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, GA 30322, USA.
William
W
Lewis
Department of Physiology and Biophysics, University of Illinois - Chicago, Chicago, IL 60612, USA.
David L
DL
Geenen
Department of Cell and Molecular Physiology, Loyola University Chicago Stritch School of Medicine, Maywood, IL 60153, USA.
Pieter
P
de Tombe
Department of Physiology, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
Paul H
PH
Goldspink
Department of Physiology and Biophysics, University of Illinois - Chicago, Chicago, IL 60612, USA.
R John
RJ
Solaro
Department of Medicine, Loyola University Chicago Stritch School of Medicine, Maywood, IL 60153, USA; Department of Cell and Molecular Physiology, Loyola University Chicago Stritch School of Medicine, Maywood, IL 60153, USA. Electronic address: asamare@luc.edu.
Allen M
AM
Samarel
D000818:Animals; D002311:Cardiomyopathy, Dilated / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D051418:Focal Adhesion Kinase 2 / Q000172:deficiency / Q000235:genetics*; D005786:Gene Expression Regulation; D006352:Heart Ventricles / Q000378:metabolism / Q000473:pathology; D008136:Longevity; D051379:Mice; D008822:Mice, Transgenic; D032383:Myocytes, Cardiac / Q000378:metabolism* / Q000473:pathology; D018995:Myosin Heavy Chains / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic; D051744:Protein Kinase C-epsilon / Q000172:deficiency / Q000235:genetics; D017434:Protein Structure, Tertiary; D019076:Transgenes; D016276:Ventricular Function / Q000502:physiology*; D020257:Ventricular Remodeling
C584019:Myh6 protein, mouse; D051418:Focal Adhesion Kinase 2; C496053:Ptk2b protein, mouse; D051744:Protein Kinase C-epsilon; D018995:Myosin Heavy Chains
Echocardiography; Focal adhesion kinase; Gene expression; Heart failure; Protein kinase C