A pilot study investigating early postoperative changes of plasma polyunsaturated fatty acids after laparoscopic sleeve gastrectomy.
BACKGROUND
This study aimed to determine early postoperative changes of plasma polyunsaturated fatty acids (PUFAs) following laparoscopic sleeve gastrectomy (LSG).


METHODS
Ten obese patients (mean BMI: 51.10 ± 11.59 kg/m²) underwent LSG and eleven normal weight control patients (mean BMI: 24.37 ± 2.33 kg/m²) underwent laparoscopic abdominal surgery. Fasting blood samples were collected prior to surgery, at day 1 after surgery and after postoperation oral feeding. Plasma levels of arachidonic acid (AA, C20:4n6), dihomo-gamma-linolenic acid (DGLA, C20:3n6), eicosapentaenoic acid (EPA, C20:5n3) and docosahexaenoic acid (DHA, C22:6n3) were determined by an optimized multiple reaction monitoring (MRM) method using ultra fast-liquid chromatography (UFLC) coupled with tandem mass spectrometry (MS/MS). Prostaglandin E₂ (PGE2) was measured in serum samples by enzyme immunoassay.


RESULTS
A significant decrease was observed in insulin and HOMA IR levels in sleeve gastrectomy patients after postoperation oral feeding compared to preoperation. Plasma AA levels and AA/EPA ratio were significantly increased in sleeve gastrectomy patients after postoperation oral feeding compared to postoperation day 1. Serum PGE2 levels and AA/DHA ratio was significantly higher in sleeve gastrectomy patients at preoperation, postoperation day 1 and after postoperation oral feeding when compared to control group patients.


CONCLUSIONS
Increased peripheral insulin sensitivity associated with LSG may play a role in the significant increase of plasma AA levels in sleeve gastrectomy patients following postoperation oral feeding. The significant increase in PGE2 levels and AA/DHA ratio in sleeve gastrectomy group patients also confirms the presence of a proinflammatory state in obesity.
Department of Medical Biochemistry, Akdeniz University Medical Faculty, Antalya, Turkey. mutayaslan@akdeniz.edu.tr.
Mutay
M
Aslan
Ibrahim
I
Aslan
Filiz
F
Özcan
Ramazan
R
Eryılmaz
Cemal Ozben
CO
Ensari
Tuna
T
Bilecik
D015126:8,11,14-Eicosatrienoic Acid / Q000097:blood; D016718:Arachidonic Acid / Q000097:blood; D015232:Dinoprostone / Q000097:blood; D004281:Docosahexaenoic Acids / Q000097:blood; D015118:Eicosapentaenoic Acid / Q000097:blood; D005231:Fatty Acids, Unsaturated / Q000097:blood*; D005743:Gastrectomy; D006801:Humans; D007328:Insulin / Q000097:blood; D010535:Laparoscopy; D009765:Obesity / Q000097:blood; D010865:Pilot Projects; D021241:Spectrometry, Mass, Electrospray Ionization
D005231:Fatty Acids, Unsaturated; D007328:Insulin; D004281:Docosahexaenoic Acids; D016718:Arachidonic Acid; D015118:Eicosapentaenoic Acid; D015126:8,11,14-Eicosatrienoic Acid; D015232:Dinoprostone