The effect of Astragalus polysaccharide on the Epstein-Barr virus lytic cycle.
Effects of a polysacharide from Chinese herbal plant Astragalus membranaceus (APS) on the expression of Epstein-Barr virus (EBV) immediate early proteins Zta, Rta and EA-D in Raji cells were examined. EBV switch from latent to lytic cycle in Raji cells was induced by 12-O-tetradecanoylphorbol-13-acetate (TPA) and sorbol butyrate (SB) and the effects of APS were examined by immunofluorescence, western blotting and flow cytometry. APS in a non-cytotoxic concentration of 30 μg/ml significantly suppressed the expression of Zta, Rta and EA-D during the EBV lytic cycle. Our observations indicate that APS is potentially useful as an anti-EBV drug.
Q
Q
Guo
X
X
Sun
Z
Z
Zhang
L
L
Zhang
G
G
Yao
F
F
Li
X
X
Yang
L
L
Song
G
G
Jiang
D000998:Antiviral Agents / Q000737:chemistry / Q000494:pharmacology*; D027883:Astragalus Plant / Q000737:chemistry*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D015967:Gene Expression Regulation, Viral / Q000187:drug effects; D004854:Herpesvirus 4, Human / Q000502:physiology*; D006801:Humans; D017874:Immediate-Early Proteins / Q000235:genetics / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D014779:Virus Replication / Q000187:drug effects
D000998:Antiviral Agents; D017874:Immediate-Early Proteins; D011134:Polysaccharides
Astragalus polysaccharide; Epstein-Barr virus; anti-EBV drug.