Association of serum cytokines levels, interleukin 10 -1082G/A and interferon-γ +874T/A polymorphisms with atopic asthma children from Saudi Arabia.
The aim of this study was to clarify the role of IL-4, IL-10, IL-13 and interferon (IFN) -γ levels in atopic asthma patients by studying the relation between their serum levels and severity of the disease. The effect of IL-10 -1082G/A and IFN-γ +874T/A SNPs was also studied. The study included 200 atopic children with asthma and 50 age- and gender matched healthy children as controls. The levels of both IL-4 and IL-13 were significantly (p<0.001) higher, while IFN-γ was significantly (p<0.001) lower in patients compared to that of the controls. There was a significant effect of gene polymorphisms of IL-10 (p<0.05) and IFN-γ (p<0.001) in occurrence of atopic asthma and increased IgE level. Polymorphism of IFN-γ gene had an effect on the serum level of IFN-γ. In conclusion, IFN-γ gene polymorphism at position +874 and IL-10 gene polymorphism at position -1082A/G are genetic determinants which contribute to susceptibility to atopic asthma in children from Saudi Arabia.
Medical Laboraotries Department, Faculty of Applied Medical Sciences, Taif University, Saudi Arabia; Medical Biochemistry Department, Faculty of Medicine, Zagazig University, Egypt. Electronic address: yousrihussein@hotmail.com.
Y M
YM
Hussein
Community Medicine Department, Faculty of Medicine, Taif University, Saudi Arabia.
S S
SS
Alzahrani
Pediatric Department, Faculty of Medicine, Taif University, Saudi Arabia.
A A
AA
Alharthi
Medical Laboraotries Department, Faculty of Applied Medical Sciences, Taif University, Saudi Arabia; Medical Microbiology & Immunology Department, Faculty of Medicine, Menoufiya University, Egypt.
M M
MM
Ghonaim
Medical Laboraotries Department, Faculty of Applied Medical Sciences, Taif University, Saudi Arabia.
A S
AS
Alhazmi
Medical Laboraotries Department, Faculty of Applied Medical Sciences, Taif University, Saudi Arabia; Medical Microbiology & Immunology Department, Faculty of Medicine, Menoufiya University, Egypt.
E M
EM
Eed
Medical Biochemistry Department, Faculty of Medicine, Zagazig University, Egypt. Electronic address: sallyshalaby@hotmail.com.
S M
SM
Shalaby
D001249:Asthma / Q000097:blood / Q000235:genetics*; D002648:Child; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D007371:Interferon-gamma / Q000097:blood* / Q000235:genetics*; D016753:Interleukin-10 / Q000097:blood* / Q000235:genetics*; D018793:Interleukin-13 / Q000097:blood; D015847:Interleukin-4 / Q000097:blood; D008297:Male; D020641:Polymorphism, Single Nucleotide; D012529:Saudi Arabia
C508609:IL10 protein, human; C578095:IL13 protein, human; C508598:IL4 protein, human; D018793:Interleukin-13; D016753:Interleukin-10; D015847:Interleukin-4; D007073:Immunoglobulin E; D007371:Interferon-gamma
Atopic asthma; Cytokines; Gene polymorphism; Genotyping; IL-10; IL-13; IL-4; Interferon γ (IFN-γ)