Effect of cold ischemia/reperfusion injury and/or shear stress with portal hypertension on the expression of matrix metalloproteinase-9.
BACKGROUND
Matrix metalloproteinase (MMP)-9 plays an important role in liver regeneration after liver surgery. MMP-9 behavior is complicated in cold ischemia/warm reperfusion injury (CIWRI) and/or shear stress with portal hypertension. Small-for-size grafts (SFSGs) are also an issue.


METHODS
We used a rat model to examine MMP-9 expression 6 h after laparotomy, a temporal clamp (Pringle maneuver), orthotopic liver transplantation (OLT) with a whole-liver graft (100% OLT), partial hepatectomy without the Pringle maneuver (60% hepatectomy) and split orthotopic liver transplantation (SOLT) with a SFSG (40% SOLT) were investigated. Four liver samples were collected in each group.


RESULTS
The normalized ratio of MMP-9 was not significantly different with a temporal clamp (P = 0.1963), 100% OLT (P = 0.1781) and 60% hepatectomy (P = 0.2367), but it was significantly higher with 40% SOLT compared to that with laparotomy (P = 0.0159).


CONCLUSIONS
Forty percent SOLT is accompanied by not only CIWRI but also shear stress. This fatal damage results in increased MMP-9 expression.
Division of Hepato-Biliary-Pancreatic, Transplant and Pediatric Surgery, Department of Surgery, Kyoto University Graduate School of Medicine, Kyoto, Japan (Tomohide Hori, Toshiyuki Hata, Kosuke Endo, Kanta Jobara, Beni Sulistiono ; Division of Transplant Surgery, Department of Transplantation, Mayo Clinic in Florida, Jacksonville, USA (Tomohide Hori, Shinji Uemoto, Feng Chen, Ann-Marie T. Baine, Lindsay B. Gardner, Justin H. Nguyen.
Tomohide
T
Hori
Division of Transplant Surgery, Department of Transplantation, Mayo Clinic in Florida, Jacksonville, USA (Tomohide Hori, Shinji Uemoto, Feng Chen, Ann-Marie T. Baine, Lindsay B. Gardner, Justin H. Nguyen.
Shinji
S
Uemoto
Division of Transplant Surgery, Department of Transplantation, Mayo Clinic in Florida, Jacksonville, USA (Tomohide Hori, Shinji Uemoto, Feng Chen, Ann-Marie T. Baine, Lindsay B. Gardner, Justin H. Nguyen.
Feng
F
Chen
Division of Transplant Surgery, Department of Transplantation, Mayo Clinic in Florida, Jacksonville, USA (Tomohide Hori, Shinji Uemoto, Feng Chen, Ann-Marie T. Baine, Lindsay B. Gardner, Justin H. Nguyen.
Marie T
MT
Ann-Baine
Division of Transplant Surgery, Department of Transplantation, Mayo Clinic in Florida, Jacksonville, USA (Tomohide Hori, Shinji Uemoto, Feng Chen, Ann-Marie T. Baine, Lindsay B. Gardner, Justin H. Nguyen.
Lindsay B
LB
Gardner
Division of Hepato-Biliary-Pancreatic, Transplant and Pediatric Surgery, Department of Surgery, Kyoto University Graduate School of Medicine, Kyoto, Japan (Tomohide Hori, Toshiyuki Hata, Kosuke Endo, Kanta Jobara, Beni Sulistiono.
Toshiyuki
T
Hata
Department of Cellular and Molecular Immunology, Mie Graduate School of Medicine, Japan (Kagemasa Kuribayashi, Takuma Kato, Kanako Saito, Linan Wang, Mie Torii).
Kagemasa
K
Kuribayashi
Department of Cellular and Molecular Immunology, Mie Graduate School of Medicine, Japan (Kagemasa Kuribayashi, Takuma Kato, Kanako Saito, Linan Wang, Mie Torii).
Takuma
T
Kato
Department of Cellular and Molecular Immunology, Mie Graduate School of Medicine, Japan (Kagemasa Kuribayashi, Takuma Kato, Kanako Saito, Linan Wang, Mie Torii).
Kanako
K
Saito
Department of Cellular and Molecular Immunology, Mie Graduate School of Medicine, Japan (Kagemasa Kuribayashi, Takuma Kato, Kanako Saito, Linan Wang, Mie Torii).
Linan
L
Wang
Department of Cellular and Molecular Immunology, Mie Graduate School of Medicine, Japan (Kagemasa Kuribayashi, Takuma Kato, Kanako Saito, Linan Wang, Mie Torii).
Mie
M
Torii
Division of Hepato-Biliary-Pancreatic, Transplant and Pediatric Surgery, Department of Surgery, Kyoto University Graduate School of Medicine, Kyoto, Japan (Tomohide Hori, Toshiyuki Hata, Kosuke Endo, Kanta Jobara, Beni Sulistiono.
Kosuke
K
Endo
Division of Hepato-Biliary-Pancreatic, Transplant and Pediatric Surgery, Department of Surgery, Kyoto University Graduate School of Medicine, Kyoto, Japan (Tomohide Hori, Toshiyuki Hata, Kosuke Endo, Kanta Jobara, Beni Sulistiono.
Kanta
K
Jobara
Division of Hepato-Biliary-Pancreatic, Transplant and Pediatric Surgery, Department of Surgery, Kyoto University Graduate School of Medicine, Kyoto, Japan (Tomohide Hori, Toshiyuki Hata, Kosuke Endo, Kanta Jobara, Beni Sulistiono.
Beni
B
Sulistiono
Division of Transplant Surgery, Department of Transplantation, Mayo Clinic in Florida, Jacksonville, USA (Tomohide Hori, Shinji Uemoto, Feng Chen, Ann-Marie T. Baine, Lindsay B. Gardner, Justin H. Nguyen.
Justin H
JH
Nguyen
Pringle maneuver; extended hepatectomy; remnant liver; small-for-size graft; split liver transplantation