A novel phospholipase A2 (D49) from the venom of the Crotalus oreganus abyssus (North American Grand canyon rattlesnake).
Currently, Crotalus viridis was divided into two species: Crotalus viridis and Crotalus oreganus. The current classification divides "the old" Crotalus viridis into two new and independent species: Crotalus viridis (subspecies: viridis and nuntius) and Crotalus oreganus (subspecies: abyssus, lutosus, concolor, oreganus, helleri, cerberus, and caliginis). The analysis of a product from cDNA (E6d), derived from the gland of a specie Crotalus viridis viridis, was found to produce an acid phospholipase A2. In this study we isolated and characterized a PLA2 (D49) from Crotalus oreganus abyssus venom. Our studies show that the PLA2 produced from the cDNA of Crotalus viridis viridis (named E6d) is exactly the same PLA2 primary sequence of amino acids isolated from the venom of Crotalus oreganus abyssus. Thus, the PLA2 from E6d cDNA is actually the same PLA2 presented in the venom of Crotalus oreganus abyssus and does not correspond to the venom from Crotalus viridis viridis. These facts highlight the importance of performing more studies on subspecies of Crotalus oreganus and Crotalus viridis, since the old classification may have led to mixed results or mistaken data.
Department of Chemistry, Biochemistry and Bioprocess Engineering, Federal University of São João Del Rei, Campus Alto Paraopeba, 34420-000 Ouro Branco, MG, Brazil.
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Martins
Department of Biochemistry of Biology Institute, State University of Campinas, 13083-970 Campinas, SP, Brazil.
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Baldasso
School of Arts, Sciences and Humanities, University of São Paulo, 03828-000 São Paulo, Brazil ; Center of Natural Sciences and Humanities, Federal University of ABC, 09210-170 Santo Andre, SP, Brazil.
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Honório
Center of Natural Sciences and Humanities, Federal University of ABC, 09210-170 Santo Andre, SP, Brazil.
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Maltarollo
Department of Chemistry, Biochemistry and Bioprocess Engineering, Federal University of São João Del Rei, Campus Alto Paraopeba, 34420-000 Ouro Branco, MG, Brazil.
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Ribeiro
Department of Chemistry, Biochemistry and Bioprocess Engineering, Federal University of São João Del Rei, Campus Alto Paraopeba, 34420-000 Ouro Branco, MG, Brazil.
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Carvalho
Center of Biomolecules Study Applied to Health, Fiocruz Rondônia, Oswaldo Cruz Foundation, Medicine Department, Federal University of Rondônia, 76812-245 Porto Velho, Brazil.
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AM
Soares
Center of Biomolecules Study Applied to Health, Fiocruz Rondônia, Oswaldo Cruz Foundation, Medicine Department, Federal University of Rondônia, 76812-245 Porto Velho, Brazil.
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Calderon
Center of Biomolecules Study Applied to Health, Fiocruz Rondônia, Oswaldo Cruz Foundation, Medicine Department, Federal University of Rondônia, 76812-245 Porto Velho, Brazil.
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Stábeli
Proteomics Platform, Barcelona Science Park, University of Barcelona, Baldiri Reixac, 08208 Barcelona, Spain.
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MA
Caballol
Institute for Research in Biomedicine, Barcelona Science Park, University of Barcelona, 08280 Barcelona, Spain ; CIBER-BBN, Barcelona Science Park, University of Barcelona, 08280 Barcelona, Spain.
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Acosta
Proteomics Platform, Barcelona Science Park, University of Barcelona, Baldiri Reixac, 08208 Barcelona, Spain.
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Oliveira
Department of Biochemistry of Biology Institute, State University of Campinas, 13083-970 Campinas, SP, Brazil.
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Marangoni
Institute for Research in Biomedicine, Barcelona Science Park, University of Barcelona, 08280 Barcelona, Spain ; CIBER-BBN, Barcelona Science Park, University of Barcelona, 08280 Barcelona, Spain ; Department of Organic Chemistry, University of Barcelona, 08028 Barcelona, Spain ; School of Chemistry and Physics, University of KwaZulu-Natal, Durban 4001, South Africa.
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Albericio
Department of Chemistry, Biochemistry and Bioprocess Engineering, Federal University of São João Del Rei, Campus Alto Paraopeba, 34420-000 Ouro Branco, MG, Brazil.
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Da Silva
D000596:Amino Acids / Q000737:chemistry*; D000818:Animals; D003435:Crotalid Venoms / Q000201:enzymology*; D017839:Crotalus; D054467:Phospholipases A2 / Q000737:chemistry* / Q000302:isolation & purification; D014481:United States
D000596:Amino Acids; D003435:Crotalid Venoms; D054467:Phospholipases A2