Targeted sonocatalytic cancer cell injury using avidin-conjugated titanium dioxide nanoparticles.
In this study, we applied sonodynamic therapy to cancer cells based on the delivery of titanium dioxide (TiO2) nanoparticles (NPs) modified with avidin protein, which preferentially discriminated cancerous cells from healthy cells. Subsequently, hydroxyl radicals were generated from the TiO2 NPs after activation by external ultrasound irradiation (TiO2/US treatment). Although 30% of the normal breast cells (human mammary epithelial cells) exhibited the uptake of avidin-modified TiO2 NPs, over 80% of the breast cancer cells (MCF-7) exhibited the uptake of avidin-TiO2 NPs. Next the effect of the TiO2/US treatment on MCF-7 cell growth was examined for up to 96 h after 1-MHz ultrasound was applied (0.1 W/cm(2), 30 s) to cells that incorporated the TiO2 NPs. No apparent cell injury was observed until 24h after the treatment, but the viable cell concentration declined to 68% compared with the control at 96 h.
Institute of Nature and Environmental Technology, Kanazawa University, Kanazawa 920-1192, Japan.
Kazuaki
K
Ninomiya
Division of Natural System, Graduate School of Natural Science and Technology, Kanazawa University, Kanazawa 920-1192, Japan.
Aya
A
Fukuda
Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Kobe 657-8501, Japan.
Chiaki
C
Ogino
Institute of Nature and Environmental Technology, Kanazawa University, Kanazawa 920-1192, Japan. Electronic address: nshimizu@staff.kanazawa-u.ac.jp.
Nobuaki
N
Shimizu
D001360:Avidin / Q000737:chemistry* / Q000627:therapeutic use*; D001943:Breast Neoplasms / Q000188:drug therapy / Q000473:pathology; D045744:Cell Line, Tumor; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D061986:MCF-7 Cells; D053758:Nanoparticles / Q000737:chemistry* / Q000627:therapeutic use*; D009369:Neoplasms / Q000628:therapy*; D014025:Titanium / Q000737:chemistry* / Q000627:therapeutic use*; D014464:Ultrasonic Therapy / Q000379:methods*
D001360:Avidin; C009495:titanium dioxide; D014025:Titanium
Avidin; Nanoparticle; Sonodynamic therapy; Titanium dioxide; Ultrasound