Olyset Duo® (a pyriproxyfen and permethrin mixture net): an experimental hut trial against pyrethroid resistant Anopheles gambiae and Culex quinquefasciatus in Southern Benin.
BACKGROUND
Alternative compounds which can complement pyrethroids on long-lasting insecticidal nets (LN) in the control of pyrethroid resistant malaria vectors are urgently needed. Pyriproxyfen (PPF), an insect growth regulator, reduces the fecundity and fertility of adult female mosquitoes. LNs containing a mixture of pyriproxyfen and pyrethroid could provide personal protection through the pyrethroid component and reduce vector abundance in the next generation through the sterilizing effect of pyriproxyfen.


METHODS
The efficacy of Olyset Duo, a newly developed mixture LN containing pyriproxyfen and permethrin, was evaluated in experimental huts in southern Benin against pyrethroid resistant Anopheles gambiae and Culex quinquefasciatus. Comparison was made with Olyset Net® (permethrin alone) and a LN with pyriproxyfen alone (PPF LN). Laboratory tunnel tests were performed to substantiate the findings in the experimental huts.


RESULTS
Overall mortality of wild pyrethroid resistant An. gambiae s.s. was significantly higher with Olyset Duo than with Olyset Net (50% vs. 27%, P = 0.01). Olyset DUO was more protective than Olyset Net (71% vs. 3%, P<0.001). The oviposition rate of surviving blood-fed An. gambiae from the control hut was 37% whereas none of those from Olyset Duo and PPF LN huts laid eggs. The tunnel test results were consistent with the experimental hut results. Olyset Duo was more protective than Olyset Net in the huts against wild pyrethroid resistant Cx. quinquefasciatus although mortality rates of this species did not differ significantly between Olyset Net and Olyset Duo. There was no sterilizing effect on surviving blood-fed Cx. quinquefasciatus with the PPF-treated nets.


CONCLUSIONS
Olyset Duo was superior to Olyset Net in terms of personal protection and killing of pyrethroid resistant An. gambiae, and sterilized surviving blood-fed mosquitoes. Mixing pyrethroid and pyriproxyfen on a LN shows potential for malaria control and management of pyrethroid resistant vectors by preventing further selection of pyrethroid resistant phenotypes.
London School of Hygiene and Tropical Medicine (LSHTM), London, United Kingdom; Centre de Recherches Entomologiques de Cotonou (CREC), Cotonou, Benin; Pan African Malaria Vector Research Consortium (PAMVERC), London, United Kingdom.
Corine
C
Ngufor
London School of Hygiene and Tropical Medicine (LSHTM), London, United Kingdom; Centre de Recherches Entomologiques de Cotonou (CREC), Cotonou, Benin; Pan African Malaria Vector Research Consortium (PAMVERC), London, United Kingdom.
Raphael
R
N'guessan
Centre de Recherches Entomologiques de Cotonou (CREC), Cotonou, Benin.
Josias
J
Fagbohoun
Centre de Recherches Entomologiques de Cotonou (CREC), Cotonou, Benin.
Abibatou
A
Odjo
Innovative Vector Control Consortium (IVCC), Liverpool, United Kingdom.
David
D
Malone
Centre de Recherches Entomologiques de Cotonou (CREC), Cotonou, Benin.
Martin
M
Akogbeto
London School of Hygiene and Tropical Medicine (LSHTM), London, United Kingdom; Pan African Malaria Vector Research Consortium (PAMVERC), London, United Kingdom.
Mark
M
Rowland
D000818:Animals; D000852:Anopheles / Q000187:drug effects*; D001541:Benin; D003465:Culex / Q000187:drug effects*; D005260:Female; D007306:Insecticides / Q000494:pharmacology*; D026023:Permethrin / Q000008:administration & dosage / Q000494:pharmacology*; D011725:Pyridines / Q000008:administration & dosage / Q000494:pharmacology*
D007306:Insecticides; D011725:Pyridines; C055613:pyriproxyfen; D026023:Permethrin