Effect of adenovirus-mediated up-regulation of α-enolase gene products on follicle-stimulating hormone receptor mRNA and luteinizing hormone receptor mRNA of granular cells from goose F1 follicles.
Enolases are glycolytic enzymes in the glycolytic pathway. In order to evaluate the effect of ENO1 on follicle-stimulating hormone receptor (FSHR) mRNA and luteinizing hormone receptor (LHR) mRNA of primary granular cell from goose F1 follicles, the recombinant plasmid adenovirus carrying ENO1 were constructed and infected the primary culture granular cells. The granular cells were randomly divided into three groups: recombinant adenovirus infected (pAd-CMV-ENO1), empty vector infected (pAd-CMV-Null) and no virus (mock control). The expression levels of FSHR mRNA and LHR mRNA of granular cells were examined by qRT-PCR. The results showed the group pAd-CMV-ENO1 had significantly higher FSHR mRNA expression levels than the other two groups (P < 0.05), but had significantly lower LHR mRNA expression levels than the other two groups (P < 0.05). The results suggested that ENO1 could improve the combination rate between FSH and FSHR to accelerate the proliferation and differentiation and steroidogenesis in poultry gonadal tissues.
College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, Jilin Province, China; College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, Heilongjiang Province, China.
Hong
H
Ji
College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, Heilongjiang Province, China.
Huanmin
H
Yang
College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, Heilongjiang Province, China.
Jianfa
J
Wang
College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, Heilongjiang Province, China.
Jingru
J
Guo
Laboratory Animal Center of Academy of Military Medical Sciences, Beijing, China.
Zhongming
Z
Hu
College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, Heilongjiang Province, China.
Hongliang
H
Zhang
College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, Heilongjiang Province, China.
Fanzhi
F
Kong
College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, Heilongjiang Province, China.
Li
L
Zhen
College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, Jilin Province, China.
Li
L
Guo
College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, Jilin Province, China. Electronic address: jihonghljbynd@aliyun.com.cn.
Juxiong
J
Liu
D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D015153:Blotting, Western / Q000662:veterinary; D005260:Female; D005777:Geese / Q000502:physiology*; D005822:Genetic Vectors / Q000235:genetics; D006107:Granulosa Cells / Q000166:cytology / Q000201:enzymology / Q000502:physiology*; D006080:Ovarian Follicle / Q000166:cytology / Q000201:enzymology / Q000502:physiology*; D010751:Phosphopyruvate Hydratase / Q000235:genetics / Q000502:physiology*; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics; D011897:Random Allocation; D011962:Receptors, FSH / Q000235:genetics / Q000502:physiology*; D011974:Receptors, LH / Q000235:genetics / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D015854:Up-Regulation / Q000235:genetics / Q000502:physiology
D012333:RNA, Messenger; D011962:Receptors, FSH; D011974:Receptors, LH; D010751:Phosphopyruvate Hydratase
ENO1; Follicle-stimulating hormone receptor; Granular cell; Luteinizing hormone receptor; Zi goose