Synergy of flavonoids with HDAC inhibitor: new approach to target Candida tropicalis biofilms.
Candida biofilms on various implanted medical devices and living tissues are serious concern in several hospital-acquired infections. The study was conducted to employ a new therapeutic strategy to inhibit the formation of Candida tropicalis biofilms. No significant antifungal or antibiofilm activity was observed when the sodium butyrate (SB), quercetin, and kaempferol were used as a lone drug. Significant decline (P<0·05) in biofilm formation was noted when sub-lethal concentration of SB (30 mM) was used in combination with the flavonoids (450 μg/ml). Z-stack analysis using 3D-confocal laser scanning microscopy (CLSM) also showed substantial reduction in the biofilm thickness in treated glass slides. In conclusion, this is the first report to our knowledge on implementing a combination therapy using flavonoids and histone deacetylase (HDAC) inhibitor.
Satish Kumar
SK
Rajasekharan
Samiraj
S
Ramesh
Dhamodharan
D
Bakkiyaraj
D000935:Antifungal Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D041022:Candida tropicalis / Q000187:drug effects*; D002177:Candidiasis / Q000188:drug therapy; D004357:Drug Synergism; D005419:Flavonoids / Q000494:pharmacology*; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology*; D006801:Humans
D000935:Antifungal Agents; D005419:Flavonoids; D056572:Histone Deacetylase Inhibitors
Antibiofilm,; C. tropicalis,; Flavonoids; Sodium butyrate,