One-pot simultaneous saccharification and fermentation: a preliminary study of a novel configuration for cellulosic ethanol production.
Combination of size reduction and mild alkali pretreatment may be a feasible way to produce bioethanol without rinsing and detoxifying the solid substrate. Based on that, a fermentation configuration named one-pot SSF in which pretreatment and fermentation steps were integrated was developed. Additionally, the effect of laccase on fermentation performance was investigated. Delignification was the major effect of the alkali pretreatment at 121°C for 60min. The highest glucose and xylose yield, which obtained from the smallest particle at a substrate loading of 2%, was 6.75 and 2.71g/L, respectively. Laccase improved the fermentation efficiency by 6.8% for one-pot SSF and 5.7% for SSF. Bioethanol from one-pot SSF with laccase supplementation reached 67.56% of the theoretical maximum, whereas that from SSF with laccase supplementation reached 57.27%. One-pot SSF might be a promising configuration to produce bioethanol because of 100% solid recovery, and rinsing water and detoxification elimination.
Research Center for Molecular Biology, Institutes of Life and Health Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, PR China. Electronic address: jingboli@jnu.edu.cn.
Jingbo
J
Li
Research Center for Molecular Biology, Institutes of Life and Health Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, PR China.
Jianghai
J
Lin
Research Center for Molecular Biology, Institutes of Life and Health Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, PR China.
Pengfei
P
Zhou
Research Center for Molecular Biology, Institutes of Life and Health Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, PR China.
Kejing
K
Wu
Research Center for Molecular Biology, Institutes of Life and Health Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, PR China.
Hongmei
H
Liu
Research Center for Molecular Biology, Institutes of Life and Health Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, PR China.
Chunjiang
C
Xiong
Research Center for Molecular Biology, Institutes of Life and Health Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, PR China.
Yingxue
Y
Gong
Research Center for Molecular Biology, Institutes of Life and Health Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, PR China.
Wenjuan
W
Xiao
Research Center for Molecular Biology, Institutes of Life and Health Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, PR China. Electronic address: zhliu@jnu.edu.cn.
Zehuan
Z
Liu
D056804:Biofuels; D019149:Bioreactors; D002482:Cellulose / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism*; D005240:Feasibility Studies; D005285:Fermentation; D006868:Hydrolysis; D010316:Particle Size; D031786:Saccharum; D012972:Sodium Hydroxide
D056804:Biofuels; D000431:Ethanol; D012972:Sodium Hydroxide; D002482:Cellulose
Bioethanol production; Enzymatic hydrolysis; Mild alkali pretreatment; One-pot SSF; Size reduction