Role of glass structure in defining the chemical dissolution behavior, bioactivity and antioxidant properties of zinc and strontium co-doped alkali-free phosphosilicate glasses.
We investigated the structure-property relationships in a series of alkali-free phosphosilicate glass compositions co-doped with Zn(2+) and Sr(2+). The emphasis was laid on understanding the structural role of Sr(2+) and Zn(2+) co-doping on the chemical dissolution behavior of glasses and its impact on their in vitro bioactivity. The structure of glasses was studied using molecular dynamics simulations in combination with solid state nuclear magnetic resonance spectroscopy. The relevant structural properties are then linked to the observed degradation behavior, in vitro bioactivity, osteoblast proliferation and oxidative stress levels. The apatite-forming ability of glasses has been investigated by X-ray diffraction, infrared spectroscopy and scanning electron microscopy-energy-dispersive spectroscopy after immersion of glass powders/bulk in simulated body fluid (SBF) for time durations varying between 1h and 14 days, while their chemical degradation has been studied in Tris-HCl in accordance with ISO 10993-14. All the glasses exhibit hydroxyapatite formation on their surface within 1-3h of their immersion in SBF. The cellular responses were observed in vitro on bulk glass samples using human osteosarcoma MG63 cell line. The dose-dependent cytoprotective effect of glasses with respect to the concentration of zinc and strontium released from the glasses is also discussed.
Department of Materials and Ceramic Engineering, University of Aveiro, CICECO, 3810-193 Aveiro, Portugal.
Saurabh
S
Kapoor
Department of Materials Science and Engineering, Rutgers, The State University of New Jersey, Piscataway, NJ 08854-8065, USA. Electronic address: ashutosh.goel@rutgers.edu.
Ashutosh
A
Goel
Department of Chemistry, University College London, 20 Gordon Street, London WC1H 0AJ, UK.
Antonio
A
Tilocca
Department of Biotechnology, DAV College, Amritsar 143-001, Punjab, India.
Vikram
V
Dhuna
Department of Molecular Biology and Biochemistry, Guru Nanak Dev University, Amritsar 143-005, Punjab, India.
Gaurav
G
Bhatia
Department of Molecular Biology and Biochemistry, Guru Nanak Dev University, Amritsar 143-005, Punjab, India.
Kshitija
K
Dhuna
Department of Materials and Ceramic Engineering, University of Aveiro, CICECO, 3810-193 Aveiro, Portugal. Electronic address: jmf@ua.pt.
José M F
JM
Ferreira
D000975:Antioxidants / Q000494:pharmacology*; D001672:Biocompatible Materials; D045744:Cell Line, Tumor; D005898:Glass; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D056004:Molecular Dynamics Simulation; D033362:Powder Diffraction; D017640:Silicates / Q000737:chemistry*; D012995:Solubility; D017550:Spectroscopy, Fourier Transform Infrared; D013324:Strontium / Q000737:chemistry*; D015032:Zinc / Q000737:chemistry*
D000975:Antioxidants; D001672:Biocompatible Materials; D017640:Silicates; D015032:Zinc; D013324:Strontium
Bioactivity; Glass; Glass structure; Strontium; Zinc