A HPLC-Q-TOF-MS-based urinary metabolomic approach to identification of potential biomarkers of metabolic syndrome.
Metabolic syndrome (MetS) is a serious threat to public health worldwide with an increased risk of developing type 2 diabetes, cardiovascular diseases and all-cause morbidity and mortality. In this study, a urinary metabolomic approach was performed on high performance liquid chromatography quadrupole time-of-flight mass spectrometry to discriminate 36 male MetS patients and 36 sex and age matched healthy controls. Pattern recognition analyses (principal component analysis and orthogonal projections to latent structures discriminate analysis) commonly demonstrated the difference between MetS patients and no-MetS subjects. This study found 8 metabolites that showed significant changes in patients with MetS, including branch-chain and aromatic amino acids (leucine, tyrosine, phenylalanine and tryptophan), short-chain acylcanitine (tiglylcarnitine), tricarboxylic acid (TCA) cycle intermediate (cis-aconitic acid) and glucuronidated products (cortolone-3-glucuronide and tetrahydroaldosterone-3-glucuronide). The candidate biomarkers revealed in this study could be useful in providing clues for further research focusing on the in-depth investigation of the cause of and cure for MetS.
Department of Occupational and Environmental Health, Tianjin Medical University, Tianjin, 300070, China.|Tianjin Entry-Exit Inspection and Quarantine Bureau, Tianjin, 300308, China.
Zhi-Rui
ZR
Yu
Department of Occupational and Environmental Health, Tianjin Medical University, Tianjin, 300070, China.|Offshore Oil Centers for Disease Control and Prevention, Tianjin, 300452, China.
Yu
Y
Ning
Department of Occupational and Environmental Health, Tianjin Medical University, Tianjin, 300070, China.
Hao
H
Yu
Department of Occupational and Environmental Health, Tianjin Medical University, Tianjin, 300070, China. tangnaijun@tijmu.edu.cn.
Nai-Jun
NJ
Tang
D000328:Adult; D015415:Biomarkers / Q000652:urine*; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D024821:Metabolic Syndrome / Q000175:diagnosis / Q000652:urine*; D055432:Metabolomics; D008875:Middle Aged; D025341:Principal Component Analysis
D015415:Biomarkers