Effects ofHelicobacter pyloriinfection and long-term proton pump inhibitor use on enterochromaffin-like cells.
BACKGROUND
Excessive release of gastrin leads to hypertrophy and hyperplasia of enterochromaffin-like cells (ECL) and prolonged stimulation of these cells causes functional impairment. The purpose of this study was to investigate the effect ofHelicobacter pylori(H. pylori)infection and long-term proton pump inhibitors (PPI) use on ECL cells.


METHODS
Fifteen patients who underwent endoscopy because of dyspeptic symptoms were enrolled in the present study. Biopsies were taken from corpus and antrum and existence ofH. pyloriwas investigated with culture, cytology and CLOtest. The patients were divided into 3 groups. Group-A:H. pylori-negative, never treated previously with PPI; Group-B:H. pylori-positive, never treated previously with PPI; and group-C:H. pylori-negative and continuously treated with PPI for more than 6 months before the subject recruitment period. The features of ECL cell in oxyntic glands were examined with electron microscopy on biopsy specimens.


RESULTS
ECL cells were completely normal in Group A. In group B, moderate hyperplasia and vacuolization was seen in ECL cells. In group C, ECL cell hyperplasia was observed and vacuoles with greater amounts of granules in enlarged vesicles were found more intensely in cytoplasm.


CONCLUSIONS
The use of PPI for a long period of time and presence ofH. pyloriinfection are risk factors for ECL hyperplasia.
Department of Gastroenterology (Mehmet Bektaş, Hülya Çetinkaya, Murat Törüner, Onur Keskin, İrfan Soykan, Esen Ismet Oktay, Esin Korkut, Yusuf Üstün, Kadir Bahar).
Mehmet
M
Bektaş
Department of Histology and Embryology (Nurşen Saraç, Esra Erdemli), Ankara University School of Medicine.
Nurşen
N
Saraç
Department of Gastroenterology (Mehmet Bektaş, Hülya Çetinkaya, Murat Törüner, Onur Keskin, İrfan Soykan, Esen Ismet Oktay, Esin Korkut, Yusuf Üstün, Kadir Bahar).
Hülya
H
Cetinkaya
Department of Gastroenterology (Mehmet Bektaş, Hülya Çetinkaya, Murat Törüner, Onur Keskin, İrfan Soykan, Esen Ismet Oktay, Esin Korkut, Yusuf Üstün, Kadir Bahar).
Murat
M
Törüner
Department of Histology and Embryology (Nurşen Saraç, Esra Erdemli), Ankara University School of Medicine.
Esra
E
Erdemli
Department of Gastroenterology (Mehmet Bektaş, Hülya Çetinkaya, Murat Törüner, Onur Keskin, İrfan Soykan, Esen Ismet Oktay, Esin Korkut, Yusuf Üstün, Kadir Bahar).
Onur
O
Keskin
Department of Gastroenterology (Mehmet Bektaş, Hülya Çetinkaya, Murat Törüner, Onur Keskin, İrfan Soykan, Esen Ismet Oktay, Esin Korkut, Yusuf Üstün, Kadir Bahar).
Irfan
I
Soykan
Department of Gastroenterology (Mehmet Bektaş, Hülya Çetinkaya, Murat Törüner, Onur Keskin, İrfan Soykan, Esen Ismet Oktay, Esin Korkut, Yusuf Üstün, Kadir Bahar).
Esen Ismet
EI
Oktay
Department of Gastroenterology (Mehmet Bektaş, Hülya Çetinkaya, Murat Törüner, Onur Keskin, İrfan Soykan, Esen Ismet Oktay, Esin Korkut, Yusuf Üstün, Kadir Bahar).
Esin
E
Korkut
Department of Gastroenterology (Mehmet Bektaş, Hülya Çetinkaya, Murat Törüner, Onur Keskin, İrfan Soykan, Esen Ismet Oktay, Esin Korkut, Yusuf Üstün, Kadir Bahar).
Yusuf
Y
Ustün
Department of Gastroenterology (Mehmet Bektaş, Hülya Çetinkaya, Murat Törüner, Onur Keskin, İrfan Soykan, Esen Ismet Oktay, Esin Korkut, Yusuf Üstün, Kadir Bahar).
Kadir
K
Bahar
Enterochromaffin-like cells; Helicobacter pylori infection; proton pump inhibitors