Poly(2-oxazoline) hydrogels as next generation three-dimensional cell supports.
Synthetic hydrogels selectively decorated with cell adhesion motifs are rapidly emerging as promising substrates for 3D cell culture. When cells are grown in 3D they experience potentially more physiologically relevant cellâ€"cell interactions and physical cues compared with traditional 2D cell culture on stiff surfaces. A newly developed polymer based on poly(2-oxazoline)s has been used for the first time to control attachment of fibroblast cells and is discussed here for its potential use in 3D cell culture with particular focus on cancer cells toward the ultimate aim of high-throughput screening of anticancer therapies. Advantages and limitations of using poly(2-oxazoline) hydrogels are discussed and compared with more established polymers, especially polyethylene glycol (PEG).
Cells and Tissue Domain; Queensland University of Technology; Institute of Health and Biomedical Innovation; Kelvin Grove; QLD, Australia.
Tim R
TR
Dargaville
Cells and Tissue Domain; Queensland University of Technology; Institute of Health and Biomedical Innovation; Translational Research Institute; QLD, Australia.
Brett G
BG
Hollier
Cells and Tissue Domain; Queensland University of Technology; Institute of Health and Biomedical Innovation; Translational Research Institute; QLD, Australia.
Ali
A
Shokoohmand
Supramolecular Chemistry Group; Department of Organic Chemistry; Ghent University; Krijgslaan 281 S4; Ghent, Belgium.
Richard
R
Hoogenboom
D002448:Cell Adhesion / Q000187:drug effects; D018929:Cell Culture Techniques / Q000379:methods*; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D009369:Neoplasms / Q000473:pathology; D010080:Oxazoles / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry
D020100:Hydrogels; D010080:Oxazoles; C577913:poly(2-oxazoline); D011092:Polyethylene Glycols
3D cell culture; cancer model; hydrogel; poly(2-oxazoline); synthetic polymer