Optimized nested polymerase chain reaction for antemortem detection of Mycobacteria in Amazon parrots (Amazona aestiva) and orange-winged Amazons (Amazona amazonica).
The objectives of this study were to optimize nested polymerase chain reaction (PCR) for Mycobacterium avium complex and Mycobacterium tuberculosis complex and apply them on samples from parrots. Results were negative for the presence of these Mycobacterium in the samples, and nested PCR was specific, faster, and more sensitive than other tests, thereby justifying its use in antemortem diagnosis.
Arianne Costa
AC
Baquião
Janaina Oliveira
JO
Luna
Aziz Orro
AO
Medina
Luiz Francisco
LF
Sanfilippo
Maria Jacinta
MJ
de Faria
Manuel Armando Azevedo
MA
dos Santos
D046708:Amazona; D000818:Animals; D001715:Bird Diseases / Q000175:diagnosis / Q000382:microbiology*; D009161:Mycobacterium / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D012680:Sensitivity and Specificity