Emerging Trichosporon asahii in elderly patients: epidemiological and molecular analysis by the DiversiLab system.
Trichosporon asahii has been recognized as an emerging opportunistic agent for invasive infections, mainly in immunocompromised patients. Urinary tract infections by this pathogen may also occur, especially in patients with urinary obstruction or those undergoing vesical catheterization and antibiotic treatment. Many outbreaks of Trichosporon spp. have been detected after urinary catheter manipulations. We report the molecular-epidemiological characterization of T. asahii in our institution using the DiversiLab system for the molecular strain typing and compare three different methods for susceptibility testing. Our results present T. asahii as an emergent pathogen in elderly patients with urinary drainage devices that can be adequately treated with triazoles, with voriconazole being the most active. Broth dilution and Vitek 2 had good concordance, while Etest showed more discrepancies. In addition, the DiversiLab system for clonal strain typing may be a useful tool for fast and accurate management of nosocomial outbreaks.
Servicio de Microbiología, Complejo Hospitalario Universitario de Santiago de Compostela, c/ Travesía Choupana s/n, 15706, Santiago de Compostela, Spain, Maria.Mercedes.Trevino.Castellano@sergas.es.
M
M
Treviño
C
C
García-Riestra
P
P
Areses
X
X
García
D
D
Navarro
F J
FJ
Suárez
I A
IA
López-Dequidt
O
O
Zaragoza
M
M
Cuenca-Estrella
D000368:Aged; D000369:Aged, 80 and over; D000935:Antifungal Agents / Q000494:pharmacology* / Q000627:therapeutic use*; D055499:Catheter-Related Infections / Q000188:drug therapy / Q000453:epidemiology / Q000382:microbiology; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D017720:Molecular Epidemiology; D058889:Molecular Typing / Q000379:methods; D016533:Mycological Typing Techniques / Q000379:methods; D014230:Triazoles / Q000494:pharmacology / Q000627:therapeutic use; D014250:Trichosporon / Q000145:classification* / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D060586:Trichosporonosis / Q000188:drug therapy / Q000453:epidemiology* / Q000382:microbiology*; D014552:Urinary Tract Infections / Q000188:drug therapy / Q000453:epidemiology / Q000382:microbiology
D000935:Antifungal Agents; D014230:Triazoles