Clear cell papillary renal cell carcinoma: micro-RNA expression profiling and comparison with clear cell renal cell carcinoma and papillary renal cell carcinoma.
Clear cell papillary renal cell carcinoma (CCPRCC) is a low-grade renal neoplasm with morphological characteristics mimicking both clear cell renal cell carcinoma (CCRCC) and papillary renal cell carcinoma (PRCC). However, despite some overlapping features, their morphological, immunohistochemical, and molecular profiles are distinct. Micro-RNAs (miRNAs) are small noncoding RNAs that play a crucial role in regulating gene expression and are involved in various biological processes, including cancer development. To better understand the biology of this tumor, we aimed to analyze the miRNA expression profile of a set of CCPRCC using microarray and quantitative reverse transcription-polymerase chain reaction. A total of 15 cases diagnosed as CCPRCC were used in this study. Among the most differentially expressed miRNA in CCPRCC, we found miR-210, miR-122, miR-34a, miR-21, miR-34b*, and miR-489 to be up-regulated, whereas miR-4284, miR-1202, miR-135a, miR-1973, and miR-204 were down-regulated compared with normal renal parenchyma. To identify consensus of differentially regulated miRNA between CCPRCC, CCRCC, and PRCC, we additionally determined differential miRNA expression using 2 publically available microarray data sets from the NCBI Gene Expression Omnibus database (GSE41282 and GSE3798). This comparison revealed that the miRNA expression profile of CCPRCC shows some overlapping characteristics between CCRCC and PRCC. Moreover, CCPRCC lacks dysregulation of important miRNAs typically associated with aggressive behavior. In summary, we describe the miRNA expression profile of a relatively infrequent type of renal cancer. Our results may help in understanding the molecular underpinning of this newly recognized entity.
Department of Pathology, Johns Hopkins University, Baltimore, MD 21287, USA.
Enrico
E
Munari
Department of Oncology, Johns Hopkins University, Baltimore, MD 21287, USA.
Luigi
L
Marchionni
Department of Oncology, Johns Hopkins University, Baltimore, MD 21287, USA.
Apurva
A
Chitre
Department of Otolaryngology, Johns Hopkins University, Baltimore, MD 21287, USA.
Masamichi
M
Hayashi
Department of Pathology and Diagnostics, University of Verona, Verona 37134, Italy; Anatomic Pathology, Pederzoli Hospital, Peschiera d/G, Verona 37019, Italy.
Guido
G
Martignoni
Department of Pathology and Diagnostics, University of Verona, Verona 37134, Italy.
Matteo
M
Brunelli
Department of Pathology and Diagnostics, University of Verona, Verona 37134, Italy; Anatomic Pathology, Pederzoli Hospital, Peschiera d/G, Verona 37019, Italy.
Stefano
S
Gobbo
Department of Pathology, Johns Hopkins University, Baltimore, MD 21287, USA.
Pedram
P
Argani
Department of Urology, Johns Hopkins University, Baltimore, MD 21287, USA.
Mohamad
M
Allaf
Department of Otolaryngology, Johns Hopkins University, Baltimore, MD 21287, USA. Electronic address: mhoque1@jhmi.edu.
Mohammad O
MO
Hoque
Department of Pathology, Johns Hopkins University, Baltimore, MD 21287, USA; Department of Oncology, Johns Hopkins University, Baltimore, MD 21287, USA; Department of Urology, Johns Hopkins University, Baltimore, MD 21287, USA. Electronic address: gnetto1@jhmi.edu.
George J
GJ
Netto
D000328:Adult; D000368:Aged; D002291:Carcinoma, Papillary / Q000235:genetics* / Q000473:pathology; D002292:Carcinoma, Renal Cell / Q000235:genetics* / Q000473:pathology; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D059467:Transcriptome
D035683:MicroRNAs
Clear cell papillary renal cell carcinoma; Micro-RNA; Profiling; Renal cancer