Effects of Lactobacillus plantarum on production performance, immune characteristics, antioxidant status, and intestinal microflora of bursin-immunized broilers.
Examples of probiotics that can promote host health by improving its intestinal microbial balance and intestinal immunity belong to the genus Lactobacillus. Bursin (BS) is a peptide isolated from the bursa of Fabricius for use as an adjuvant for a variety of immunogens. To investigate the synergistic effects of Lactobacillus plantarum (LP) dietary supplementation and BS immunization on production performance, immune characteristics, antioxidant status, and intestinal microflora in broilers, we randomly allocated 200 1-day-old broilers of mixed sex into 4 treatments in a 2 × 2 factorial arrangement (LP-/BS-, LP-/BS+, LP+/BS-, LP+/BS+) for 42 days. BS immunization enhanced immune response by increasing serum total immunoglobulin G concentration and interleukin-6 concentration, promoted antioxidant capacity by increasing catalase activities in serum and liver and by decreasing serum malondialdehyde (MDA) content at 42 days of age (DOA), and enriched intestinal microflora diversity. LP supplementation enhanced immune response by increasing interleukin-2 concentration at 42 DOA; promoted antioxidant capacity by increasing liver catalase activities, increasing glutathione peroxidase activities in serum and liver at 21 DOA, and decreasing serum MDA content at 42 DOA; promoted intestinal microflora composition by decreasing total aerobes and Escherichia coli counts at 21 DOA, by increasing total anaerobes count at 21 DOA, and by increasing Lactobacillus spp. and Bifidobacterium spp. counts at both 21 and 42 DOA. The interactions between BS and LP had a significant effect on daily body mass gain and feed conversion ratio in the starter period (1-21 DOA); on interleukin-2 concentration and liver MDA content at 21 DOA; and on thymus index, peripheral lymphocyte proliferation, and E. coli counts at 42 DOA. Overall, these data suggest that the combination of LP dietary supplementation and BS immunization promoted the production performance, immune characteristics, antioxidant status, and intestinal microflora of broilers.
Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Xinkang 46, Ya'an 625014, People's Republic of China.
Xuejiao
X
Shen
Dan
D
Yi
Xueqin
X
Ni
Dong
D
Zeng
Bo
B
Jing
Mingxia
M
Lei
Zhengrong
Z
Bian
Yan
Y
Zeng
Tao
T
Li
Jinge
J
Xin
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001644:Bifidobacterium / Q000254:growth & development; D002645:Chickens / Q000254:growth & development / Q000276:immunology / Q000382:microbiology / Q000502:physiology*; D019587:Dietary Supplements; D016983:Enterococcus / Q000254:growth & development; D004926:Escherichia coli / Q000254:growth & development; D005260:Female; D007114:Immunization / Q000662:veterinary; D007074:Immunoglobulin G / Q000097:blood; D007376:Interleukin-2 / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D007422:Intestines / Q000187:drug effects / Q000382:microbiology; D007778:Lactobacillus / Q000254:growth & development; D048191:Lactiplantibacillus plantarum / Q000502:physiology*; D008099:Liver / Q000201:enzymology / Q000378:metabolism; D008214:Lymphocytes / Q000166:cytology / Q000276:immunology; D008297:Male; D009842:Oligopeptides / Q000276:immunology*; D019936:Probiotics / Q000008:administration & dosage; D011897:Random Allocation
D000975:Antioxidants; D007074:Immunoglobulin G; D007376:Interleukin-2; D015850:Interleukin-6; D009842:Oligopeptides; C016281:bursopoietin