Salinity stress induced lipid synthesis to harness biodiesel during dual mode cultivation of mixotrophic microalgae.
Influence of salinity as a stress factor to harness biodiesel was assessed during dual mode cultivation of microalgae by integrating biomass growth phase (BGP) and salinity induced lipid induction phase (LIP). BGP was evaluated in mixotrophic mode employing nutrients (NPK) and carbon (glucose) source while LIP was operated under stress environment with varying salt concentrations (0, 0.5, 1 and 2gNaCl/l). Salinity stress triggered both biomass growth and lipid synthesis in microalgae significantly. BGP showed higher increments in biomass growth (2.55g/l) while LIP showed higher lipid productivity (1gNaCl/l; total/neutral lipid, 23.4/9.2%) than BGP (total/neutral lipid, 15.2/6%). Lower concentrations of salinity showed positive influence on the process while higher concentrations showed marked inhibition. Salinity stress also facilitated in maintaining saturated fatty acid methyl esters in higher amounts which associates with the improved fuel properties. Efficient wastewater treatment was observed during BGP operation indicating the assimilation of carbon/nutrients by microalgae.
Bioengineering and Environmental Centre (BEEC), CSIR-Indian Institute of Chemical Technology (CSIR-IICT), Hyderabad 500 007, India. Electronic address: vmohan_s@yahoo.com.
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S
Venkata Mohan
Bioengineering and Environmental Centre (BEEC), CSIR-Indian Institute of Chemical Technology (CSIR-IICT), Hyderabad 500 007, India.
M Prathima
MP
Devi
D056804:Biofuels; D057919:Biological Oxygen Demand Analysis; D018533:Biomass; D001709:Biotechnology; D050260:Carbohydrate Metabolism / Q000187:drug effects; D018929:Cell Culture Techniques / Q000379:methods*; D002734:Chlorophyll / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D005232:Fatty Acids, Volatile / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D008055:Lipids / Q000096:biosynthesis*; D058086:Microalgae / Q000254:growth & development* / Q000378:metabolism*; D009566:Nitrates / Q000302:isolation & purification; D010710:Phosphates / Q000302:isolation & purification; D054712:Salinity; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological; D062065:Wastewater / Q000737:chemistry; D018508:Water Purification
D056804:Biofuels; D002734:Chlorophyll; D005227:Fatty Acids; D005232:Fatty Acids, Volatile; D008055:Lipids; D009566:Nitrates; D010710:Phosphates; D012965:Sodium Chloride; D062065:Wastewater
Carbohydrates; Fatty acid methyl esters (FAME); Neutral lipid; Saturated fatty acids; Wastewater treatment