Archaeal amoA and ureC genes and their transcriptional activity in the Arctic Ocean.
Thaumarchaeota and the gene encoding for a subunit of ammonia monooxygenase (amoA) are ubiquitous in Polar Seas, and some Thaumarchaeota also have a gene coding for ureC, diagnostic for urease. Using quantitative PCR we investigated the occurrence of genes and transcripts of ureC and amoA in Arctic samples from winter, spring and summer. AmoA genes, ureC genes and amoA transcripts were always present, but ureC transcripts were rarely detected. Over a 48 h light manipulation experiment amoA transcripts persisted under light and dark conditions, but not ureC transcripts. In addition, maxima for amoA transcript were nearer the surface compared to amoA genes. Clone libraries using DNA template recovered shallow and deep amoA clades but only the shallow clade was recovered from cDNA (from RNA). These results imply environmental control of amoA expression with direct or indirect light effects, and rare ureC expression despite its widespread occurrence in the Arctic Ocean.
Québec-Océan and Département de Biologie, Université Laval, Québec QC G1V 0A6, Canada.
Estelle
E
Pedneault
1] Sorbonne Universités, UPMC, Univ Paris 06, UMR8222, Observatoire Océanologique, Banyuls-sur-Mer, France [2] CNRS, UMR 8222, Laboratoire d'écogéochimie des environnements benthiques (LECOB), Observatoire Océanologique, Banyuls-sur-Mer, France.
Pierre E
PE
Galand
1] Québec-Océan and Département de Biologie, Université Laval, Québec QC G1V 0A6, Canada [2] Institut de Biologie Intégrative et des Systèmes (IBIS), Université Laval, Québec QC G1V 0A6, Canada.
Marianne
M
Potvin
Québec-Océan and Département de Biologie, Université Laval, Québec QC G1V 0A6, Canada.
Jean-Éric
JÉ
Tremblay
1] Québec-Océan and Département de Biologie, Université Laval, Québec QC G1V 0A6, Canada [2] Institut de Biologie Intégrative et des Systèmes (IBIS), Université Laval, Québec QC G1V 0A6, Canada.
Connie
C
Lovejoy
D001105:Archaea / Q000235:genetics*; D019843:Archaeal Proteins / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D001110:Arctic Regions; D020869:Gene Expression Profiling; D009566:Nitrates / Q000378:metabolism; D009792:Oceans and Seas; D010088:Oxidoreductases / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic; D014510:Urease / Q000145:classification / Q000235:genetics* / Q000378:metabolism
D019843:Archaeal Proteins; D009566:Nitrates; D012336:RNA, Ribosomal, 16S; D010088:Oxidoreductases; C045582:ammonia monooxygenase; D014510:Urease