Effects of atorvastatin on systemic and renal NO dependency in patients with non-diabetic stage II-III chronic kidney disease.
OBJECTIVE
Clinical trials suggest that statins have beneficial effects on the cardiovascular system independent from their cholesterol lowering properties. In patients with chronic kidney disease stage II-III, we tested the hypothesis that atorvastatin increased systemic and renal nitric oxide (NO) availability using L-N(G) -monomethyl arginine (L-NMMA) as an inhibitor of NO production.


METHODS
In a randomized, placebo-controlled, crossover study patients were treated with atorvastatin for 5 days with standardized diet and fluid intake. Glomerular filtration reate (GFR), fractional excretions of sodium (FENa ), urinary excretion of aquaporin-2 (u-AQP2) and epithelial sodium channels (u-ENaCγ ), vasoactive hormones (renin, angiotensin II, aldosterone, arginine vasopressin, endothelin-1 and brain natriuretic peptide) and central blood pressure (BP) estimated by applanation tonometry were measured before and after systemic administration of the NO inhibitor L-NMMA.


RESULTS
Atorvastatin caused a significant reduction in U-ENaCγ , but sodium excretion, C H 2 O , FENa and u-AQP2 were not changed by atorvastatin. L-NMMA reduced renal effect variables, including GFR, FENa and u-ENaCγ and increased brachial BP and central BP to a similar extent during both treatments. Vasoactive hormones were changed in the same way by L-NMMA during atorvastatin and placebo treatment.


CONCLUSIONS
During, atorvastatin and placebo treatment, inhibition of nitric oxide synthesis induced the same response in brachial and central blood pressure, GFR, renal tubular function and vasoactive hormones. Thus, the data do not support that atorvastatin changes nitric oxide availability in patients with mild nephropathy. The reduced u-ENaC may reflect changes in sodium absorption in the nephron induced by atorvastatin.
Department of Medical Research, University Clinic in Hypertension and Nephrology, Holstebro Hospital, Holstebro, Denmark.
Frank Holden
FH
Mose
Thomas
T
Larsen
Janni Majgaard
JM
Jensen
Annebirthe Bo
AB
Hansen
Jesper Nørgaard
JN
Bech
Erling Bjerregaard
EB
Pedersen
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000069059:Atorvastatin; D001794:Blood Pressure; D018592:Cross-Over Studies; D004311:Double-Blind Method; D053503:Epithelial Sodium Channels / Q000032:analysis; D005260:Female; D005919:Glomerular Filtration Rate / Q000187:drug effects; D006538:Heptanoic Acids / Q000494:pharmacology*; D006801:Humans; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors / Q000494:pharmacology*; D007668:Kidney / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009569:Nitric Oxide / Q000502:physiology*; D011758:Pyrroles / Q000494:pharmacology*; D051436:Renal Insufficiency, Chronic / Q000378:metabolism*; D059289:Vascular Stiffness; D019323:omega-N-Methylarginine / Q000494:pharmacology
D053503:Epithelial Sodium Channels; D006538:Heptanoic Acids; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D011758:Pyrroles; D019323:omega-N-Methylarginine; D009569:Nitric Oxide; D000069059:Atorvastatin
atorvastatin; chronic kidney disease; epithelial sodium channels; fractional excretion of sodium; nitric oxide