Detection of prosthetic joint infection by use of PCR-electrospray ionization mass spectrometry applied to synovial fluid.
PCR coupled with electrospray ionization mass spectrometry applied to synovial fluid specimens had an 81% sensitivity and a 95% specificity for the diagnosis of prosthetic joint infection.
Division of Infectious Diseases, Department of Medicine, Mayo Clinic, Rochester, Minnesota, USA.
Dante P
DP
Melendez
Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota, USA.
James R
JR
Uhl
Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota, USA.
Kerryl E
KE
Greenwood-Quaintance
Department of Orthopedic Surgery, Mayo Clinic, Rochester, Minnesota, USA.
Arlen D
AD
Hanssen
Ibis Biosciences, Abbott Company, Carlsbad, California, USA.
Rangarajan
R
Sampath
Division of Infectious Diseases, Department of Medicine, Mayo Clinic, Rochester, Minnesota, USA Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota, USA Department of Orthopedic Surgery, Mayo Clinic, Rochester, Minnesota, USA patel.robin@mayo.edu.
Robin
R
Patel
D000368:Aged; D000369:Aged, 80 and over; D001168:Arthritis / Q000175:diagnosis*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*; D016459:Prosthesis-Related Infections / Q000175:diagnosis*; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013582:Synovial Fluid / Q000382:microbiology*