Ultrafast Structural Dynamics of BlsA, a Photoreceptor from the Pathogenic BacteriumAcinetobacter baumannii.
Acinetobacter baumanniiis an important human pathogen that can form biofilms and persist under harsh environmental conditions. Biofilm formation and virulence are modulated by blue light, which is thought to be regulated by a BLUF protein, BlsA. To understand the molecular mechanism of light sensing, we have used steady-state and ultrafast vibrational spectroscopy to compare the photoactivation mechanism of BlsA to the BLUF photosensor AppA fromRhodobacter sphaeroides. Although similar photocycles are observed, vibrational data together with homology modeling identify significant differences in the β5 strand in BlsA caused by photoactivation, which are proposed to be directly linked to downstream signaling.
Department of Chemistry, Stony Brook University , Stony Brook, New York 11794-3400, United States.
Richard
R
Brust
Department of Chemistry, Stony Brook University , Stony Brook, New York 11794-3400, United States.
Allison
A
Haigney
School of Chemistry, University of East Anglia , Norwich Research Park, Norwich NR4 7TJ, United Kingdom ; Department of Biophysics, Medical School, University of Pecs , Pecs, Hungary.
Andras
A
Lukacs
Department of Chemistry, Stony Brook University , Stony Brook, New York 11794-3400, United States.
Agnieszka
A
Gil
Department of Chemistry, Stony Brook University , Stony Brook, New York 11794-3400, United States.
Shahrier
S
Hossain
School of Chemistry, University of East Anglia , Norwich Research Park, Norwich NR4 7TJ, United Kingdom.
Kiri
K
Addison
Department of Chemistry, Stony Brook University , Stony Brook, New York 11794-3400, United States.
Cheng-Tsung
CT
Lai
Central Laser Facility, Research Complex at Harwell , Harwell Science and Innovation Campus, Didcot, Oxon OX11 0QX, United Kingdom.
Michael
M
Towrie
Central Laser Facility, Research Complex at Harwell , Harwell Science and Innovation Campus, Didcot, Oxon OX11 0QX, United Kingdom.
Gregory M
GM
Greetham
Central Laser Facility, Research Complex at Harwell , Harwell Science and Innovation Campus, Didcot, Oxon OX11 0QX, United Kingdom.
Ian P
IP
Clark
Institut für Biochemie und Lebensmittelchemie, Universität Hamburg , Grindelallee 117, D-20146 Hamburg, Germany.
Boris
B
Illarionov
Institut für Biochemie und Lebensmittelchemie, Universität Hamburg , Grindelallee 117, D-20146 Hamburg, Germany.
Adelbert
A
Bacher
Institut für Biochemie und Lebensmittelchemie, Universität Hamburg , Grindelallee 117, D-20146 Hamburg, Germany.
Ryu-Ryun
RR
Kim
Institut für Biochemie und Lebensmittelchemie, Universität Hamburg , Grindelallee 117, D-20146 Hamburg, Germany.
Markus
M
Fischer
Department of Chemistry, Stony Brook University , Stony Brook, New York 11794-3400, United States.
Carlos
C
Simmerling
School of Chemistry, University of East Anglia , Norwich Research Park, Norwich NR4 7TJ, United Kingdom.
Stephen R
SR
Meech
Department of Chemistry, Stony Brook University , Stony Brook, New York 11794-3400, United States.
Peter J
PJ
Tonge