Antifungal and antibacterial metabolites from an endophytic Aspergillus sp. associated with Melia azedarach.
Seven known metabolites, dianhydro-aurasperone C (1), isoaurasperone A (2), fonsecinone A (3), asperpyrone A (4), asperazine (5), rubrofusarin B (6) and (R)-3-hydroxybutanonitrile (7), were isolated from the culture of Aspergillus sp. KJ-9, a fungal endophyte isolated from Melia azedarach and identified by spectroscopic methods. All isolates were evaluated in vitro against several phytopathogenic fungi (Gibberella saubinetti, Magnaporthe grisea, Botrytis cinerea, Colletotrichum gloeosporioides and Alternaria solani) and pathogenic bacteria (Escherichia coli, Bacillus subtilis, Staphyloccocus aureus and Bacillus cereus). Compounds 3 and 7 were active against almost all phytopathogenic fungi tested with minimum inhibitory concentration (MIC) range of 6.25-50 μM. Moreover, compound 3 was active against all pathogenic bacteria with MIC in the range of 25-100 μM. Compound 7 is a rare new natural product isolated from a natural source for the first time, and the detailed NMR data of 1 were first assigned.
a College of Science, Shaanxi Engineering Center of Bioresource Chemistry & Sustainable Utilization, Northwest A&F University , Yangling, Shaanxi 712100 , P.R. China.
Jian
J
Xiao
Qiang
Q
Zhang
Yu-Qi
YQ
Gao
Xin-Wei
XW
Shi
Jin-Ming
JM
Gao
D000528:Alternaria / Q000187:drug effects; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D001230:Aspergillus / Q000737:chemistry*; D001409:Bacillus cereus / Q000187:drug effects; D001412:Bacillus subtilis / Q000187:drug effects; D020171:Botrytis / Q000187:drug effects; D002867:Chromones / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D020231:Colletotrichum / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects; D005874:Gibberella / Q000187:drug effects; D007211:Indoles / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D020082:Magnaporthe / Q000187:drug effects; D031586:Melia azedarach / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D009281:Naphthalenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D009570:Nitriles / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D019906:Nuclear Magnetic Resonance, Biomolecular; D010879:Piperazines / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D011753:Pyrones / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects
C000593286:(R)-3-hydroxybutanonitrile; D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D002867:Chromones; D007211:Indoles; D009281:Naphthalenes; D009570:Nitriles; D010879:Piperazines; D011753:Pyrones; C437185:asperazine; C507895:fonsecinone A
Aspergillus sp; antimicrobial bioactivity; endophytic fungus; naphtho-γ-pyrone polyketide; phytopathogenic fungi