Hypoxia-induced miR-210 in epithelial ovarian cancer enhances cancer cell viability via promoting proliferation and inhibiting apoptosis.
miR-210 is upregulated in a HIF-1α-dependent way in several types of cancers. In addition, upregulated miR-210 promotes cancer proliferation, via its anti-apoptotic effects. It is blind to the regulation of miR-210 under hypoxia conditions for ovarian cancer cells and to the effect of miR-210 on ovarian cancer growth. In the present study, we determined the expression of miR-210 in epithelial ovarian cancer specimens, and in ovarian cancer cell lines under hypoxia conditions, and determined in detail the effect of miR-210 overexpression on tumor cell proliferation, and the possible mechanisms of tumor growth by miR-210 regulation. It was shown that miR-210 expression is upregulated, in response to hypoxia conditions in epithelial ovarian cancer specimens as well as epithelial ovarian cancer cell lines, with an association to HIF-1α overexpression. Furthermore, upregulated miR-210 promoted tumor growth in vitro via targeting PTPN1 and inhibiting apoptosis. Therefore, our findings shed light on the mechanism of ovarian cancer adaptation to hypoxia.
Department of Obstetrics and Gynecology, Chinese PLA General Hospital, Beijing 100853, P.R. China.
Li'an
L
Li
Department of Obstetrics and Gynecology, Chinese PLA General Hospital, Beijing 100853, P.R. China.
Ke
K
Huang
Department of Obstetrics and Gynecology, Chinese PLA General Hospital, Beijing 100853, P.R. China.
Yanqin
Y
You
Department of Obstetrics and Gynecology, Chinese PLA General Hospital, Beijing 100853, P.R. China.
Xiaoyu
X
Fu
Department of Obstetrics and Gynecology, Chinese PLA General Hospital, Beijing 100853, P.R. China.
Lingyun
L
Hu
Department of Obstetrics and Gynecology, Chinese PLA General Hospital, Beijing 100853, P.R. China.
Lei
L
Song
Department of Obstetrics and Gynecology, Chinese PLA General Hospital, Beijing 100853, P.R. China.
Yuanguang
Y
Meng
D017209:Apoptosis; D000077216:Carcinoma, Ovarian Epithelial; D015687:Cell Hypoxia; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D009375:Neoplasms, Glandular and Epithelial / Q000235:genetics / Q000473:pathology*; D010051:Ovarian Neoplasms / Q000235:genetics / Q000473:pathology*; D010100:Oxygen / Q000378:metabolism*; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1 / Q000378:metabolism*
C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; C543800:MIRN210 microRNA, human; D035683:MicroRNAs; C516607:PTPN1 protein, human; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1; D010100:Oxygen