Threshold sensing through a synthetic enzymatic reaction-diffusion network.
A wet stamping method to precisely control concentrations of enzymes and inhibitors in place and time inside layered gels is reported. By combining enzymatic reactions such as autocatalysis and inhibition with spatial delivery of components through soft lithographic techniques, a biochemical reaction network capable of recognizing the spatial distribution of an enzyme was constructed. The experimental method can be used to assess fundamental principles of spatiotemporal order formation in chemical reaction networks.
Department of Physical Organic Chemistry, Radboud University Nijmegen, Institute for Molecules and Materials, Heyendaalseweg 135, 6525 AJ Nijmegen (The Netherlands) http://www.ru.nl/physicalorganicchemistry/
Sergey N
SN
Semenov
Albert J
AJ
Markvoort
Tom F A
TF
de Greef
Wilhelm T S
WT
Huck
D002384:Catalysis; D004058:Diffusion; D004798:Enzymes / Q000737:chemistry*
D004798:Enzymes
biochemical networks; biosensors; enzyme catalysis; reaction-diffusion systems; ultrasensitivity