Polyacrylamide-based biocompatible Nanoplatform enhances the tumor uptake, PET/fluorescence imaging and anticancer activity of a chlorophyll analog.
In this report we demonstrate the outstanding advantages of multifunctional nanoplatforms for cancer-imaging and therapy. The non-toxic polyacrylamide (PAA) nanoparticles (size:18-25 nm) formulation drastically changed the pharmacokinetic profile of the ¹²⁴I- labeled chlorophyll-a derivative (formulated in 10% ethanol in PBS) with a remarkable enhancement in tumor uptake, and significantly reduced uptake in spleen and liver. Among the various nanoformulations investigated, the ¹²⁴I- labeled photosensitizer (dose: 0.6142 MBq), and the cyanine dye-nanoparticles (CD-NP) conjugate (dose 0.3 μmol/kg) in combination showed great potential for tumor imaging (PET/NIR fluorescence) in BALB/c mice bearing Colon26 tumors. Compared to free non-labeled photosensitizer, the corresponding PAA nanoformulation under similar treatment parameters showed a remarkable enhancement in long-term tumor cure by PDT (photodynamic therapy) and provides an opportunity to develop a single nanoplatform for tumor-imaging (PET/fluorescence) and phototherapy, a practical "See and Treat" approach.
1. PDT Center, Cell Stress Biology, Roswell Park Cancer Institute, Buffalo, NY 14263, USA;
Anurag
A
Gupta
2. Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109, USA;
Shouyan
S
Wang
1. PDT Center, Cell Stress Biology, Roswell Park Cancer Institute, Buffalo, NY 14263, USA;
Aimee
A
Marko
1. PDT Center, Cell Stress Biology, Roswell Park Cancer Institute, Buffalo, NY 14263, USA;
Penny
P
Joshi
1. PDT Center, Cell Stress Biology, Roswell Park Cancer Institute, Buffalo, NY 14263, USA;
Manivannan
M
Ethirajan
1. PDT Center, Cell Stress Biology, Roswell Park Cancer Institute, Buffalo, NY 14263, USA;
Yihui
Y
Chen
3. Department of Nuclear Medicine, State University of New York, Buffalo, NY 14032, USA.
Rutao
R
Yao
3. Department of Nuclear Medicine, State University of New York, Buffalo, NY 14032, USA.
Munawwar
M
Sajjad
2. Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109, USA;
Raoul
R
Kopelman
1. PDT Center, Cell Stress Biology, Roswell Park Cancer Institute, Buffalo, NY 14263, USA;
Ravindra K
RK
Pandey
D000818:Animals; D002734:Chlorophyll / Q000031:analogs & derivatives* / Q000737:chemistry / Q000493:pharmacokinetics / Q000627:therapeutic use; D003110:Colonic Neoplasms / Q000000981:diagnostic imaging* / Q000188:drug therapy; D007457:Iodine Radioisotopes / Q000737:chemistry / Q000493:pharmacokinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D053758:Nanoparticles / Q000737:chemistry* / Q000627:therapeutic use; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000737:chemistry* / Q000493:pharmacokinetics / Q000627:therapeutic use; D049268:Positron-Emission Tomography; D041622:Tomography, Optical
D007457:Iodine Radioisotopes; D017319:Photosensitizing Agents; C505556:methyl 3-(1'-3-iodobenzyloxyethyl)-3-devinylpyropheophorbide-a; D002734:Chlorophyll
Nanoparticles; Positron Imaging Tomography; Reactive Oxygen species.; photodynamic therapy