Usnic acid-loaded biocompatible magnetic PLGA-PVA microsphere thin films fabricated by MAPLE with increased resistance to staphylococcal colonization.
Due to their persistence and resistance to the current therapeutic approaches, Staphylococcus aureus biofilm-associated infections represent a major cause of morbidity and mortality in the hospital environment. Since (+)-usnic acid (UA), a secondary lichen metabolite, possesses antimicrobial activity against Gram-positive cocci, including S. aureus, the aim of this study was to load magnetic polylactic-co-glycolic acid-polyvinyl alcohol (PLGA-PVA) microspheres with UA, then to obtain thin coatings using matrix-assisted pulsed laser evaporation and to quantitatively assess the capacity of the bio-nano-active modified surface to control biofilm formation by S. aureus, using a culture-based assay. The UA-loaded microspheres inhibited both the initial attachment of S. aureus to the coated surfaces, as well as the development of mature biofilms. In vitro bioevalution tests performed on the fabricated thin films revealed great biocompatibility, which may endorse them as competitive candidates for the development of improved non-toxic surfaces resistant to S. aureus colonization and as scaffolds for stem cell cultivation and tissue engineering.
Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, Polizu Street no 1-7, 011061 Bucharest, Romania. Lasers Department, Plasma and Radiation Physics, National Institute for Lasers, PO Box MG-36, Bucharest-Magurele, Romania.
V
V
Grumezescu
A M
AM
Holban
A M
AM
Grumezescu
G
G
Socol
A
A
Ficai
B S
BS
Vasile
R
R
Truscă
C
C
Bleotu
V
V
Lazar
C M
CM
Chifiriuc
G D
GD
Mogosanu
D001572:Benzofurans / Q000737:chemistry / Q000494:pharmacology*; D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000737:chemistry; D018441:Biofilms / Q000187:drug effects; D004337:Drug Carriers / Q000138:chemical synthesis* / Q000737:chemistry; D016503:Drug Delivery Systems / Q000295:instrumentation; D024881:Drug Resistance, Bacterial; D019344:Lactic Acid / Q000138:chemical synthesis* / Q000737:chemistry; D008863:Microspheres; D011100:Polyglycolic Acid / Q000138:chemical synthesis* / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000502:physiology
D001572:Benzofurans; D001672:Biocompatible Materials; D004337:Drug Carriers; C073339:usnic acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid