Tomographic phase microscopy of living three-dimensional cell cultures.
A successful application of self-interference digital holographic microscopy in combination with a sample-rotation-based tomography module for three-dimensional (3-D) label-free quantitative live cell imaging with subcellular resolution is demonstrated. By means of implementation of a hollow optical fiber as the sample cuvette, the observation of living cells in different 3-D matrices is enabled. The fiber delivers a stable and accurate rotation of a cell or cell cluster, providing quantitative phase data for tomographic reconstruction of the 3-D refractive index distribution with an isotropic spatial resolution. We demonstrate that it is possible to clearly distinguish and quantitatively analyze several cells grouped in a "3-D cluster" as well as subcellular organelles like the nucleoli and local internal refractive index changes.
Warsaw University of Technology, Institute of Micromechanics and Photonics, Photonics Engineering Division, Faculty of Mechatronics, Sw. A. Boboli 8 Street, 02-525 Warsaw, Poland.
Arkadiusz
A
Kuś
Warsaw University of Technology, Institute of Micromechanics and Photonics, Photonics Engineering Division, Faculty of Mechatronics, Sw. A. Boboli 8 Street, 02-525 Warsaw, Poland.
Michał
M
Dudek
University of Münster, Center for Biomedical Optics and Photonics, Robert-Koch-Strasse 45, 48149 Münster, GermanycUniversity of Münster, Biomedical Technology Center of the Medical Faculty, Mendelstraße 17 17, 48149 Münster, Germany.
Björn
B
Kemper
Warsaw University of Technology, Institute of Micromechanics and Photonics, Photonics Engineering Division, Faculty of Mechatronics, Sw. A. Boboli 8 Street, 02-525 Warsaw, Poland.
Małgorzata
M
Kujawińska
University of Münster, Center for Biomedical Optics and Photonics, Robert-Koch-Strasse 45, 48149 Münster, Germany.
Angelika
A
Vollmer
D018929:Cell Culture Techniques / Q000379:methods*; D045744:Cell Line, Tumor; D004867:Equipment Design; D006696:Holography / Q000379:methods*; D006801:Humans; D012031:Refractometry; D041622:Tomography, Optical / Q000379:methods*