Rapid and efficient conversion of integration-free human induced pluripotent stem cells to GMP-grade culture conditions.
Data suggest that clinical applications of human induced pluripotent stem cells (hiPSCs) will be realized. Nonetheless, clinical applications will require hiPSCs that are free of exogenous DNA and that can be manufactured through Good Manufacturing Practice (GMP). Optimally, derivation of hiPSCs should be rapid and efficient in order to minimize manipulations, reduce potential for accumulation of mutations and minimize financial costs. Previous studies reported the use of modified synthetic mRNAs to reprogram fibroblasts to a pluripotent state. Here, we provide an optimized, fully chemically defined and feeder-free protocol for the derivation of hiPSCs using synthetic mRNAs. The protocol results in derivation of fully reprogrammed hiPSC lines from adult dermal fibroblasts in less than two weeks. The hiPSC lines were successfully tested for their identity, purity, stability and safety at a GMP facility and cryopreserved. To our knowledge, as a proof of principle, these are the first integration-free iPSCs lines that were reproducibly generated through synthetic mRNA reprogramming that could be putatively used for clinical purposes.
Institute for Stem Cell Biology and Regenerative Medicine, Department of Obstetrics and Gynecology and Department of Genetics, Stanford University, Stanford, California, United States of America.
Jens
J
Durruthy-Durruthy
Institute for Stem Cell Biology and Regenerative Medicine, Department of Obstetrics and Gynecology and Department of Genetics, Stanford University, Stanford, California, United States of America.
Sharon F
SF
Briggs
Eli and Edythe Broad Center for Regenerative Medicine & Stem Cell Research, University of California Los Angeles, Los Angeles, California, United States of America; Department of Molecular and Medical Pharmacology, University of California Los Angeles, Los Angeles, California, United States of America.
Jason
J
Awe
Institute for Stem Cell Biology and Regenerative Medicine, Department of Obstetrics and Gynecology and Department of Genetics, Stanford University, Stanford, California, United States of America.
Cyril Y
CY
Ramathal
Eli and Edythe Broad Center for Regenerative Medicine & Stem Cell Research, University of California Los Angeles, Los Angeles, California, United States of America; Department of Microbiology, Immunology and Molecular Genetics, University of California Los Angeles, Los Angeles, California, United States of America.
Saravanan
S
Karumbayaram
Eli and Edythe Broad Center for Regenerative Medicine & Stem Cell Research, University of California Los Angeles, Los Angeles, California, United States of America; Department of Molecular and Medical Pharmacology, University of California Los Angeles, Los Angeles, California, United States of America.
Patrick C
PC
Lee
Veterans Affairs Palo Alto Health Care System, Stanford University, Palo Alto, California, United States of America.
Julia D
JD
Heidmann
Eli and Edythe Broad Center for Regenerative Medicine & Stem Cell Research, University of California Los Angeles, Los Angeles, California, United States of America; Department of Molecular, Cellular, and Developmental Biology, Molecular Biology Institute, University of California Los Angeles, Los Angeles, California, United States of America.
Amander
A
Clark
Stanford Cardiovascular Institute, Stanford University, Stanford, California, United States of America; Department of Cardiology, Department of Medicine, Stanford University, Stanford, California, United States of America.
Ioannis
I
Karakikes
Department of Developmental Biology, Stanford University, Stanford, California, United States of America.
Kyle M
KM
Loh
Stanford Cardiovascular Institute, Stanford University, Stanford, California, United States of America; Department of Cardiology, Department of Medicine, Stanford University, Stanford, California, United States of America.
Joseph C
JC
Wu
Veterans Affairs Palo Alto Health Care System, Stanford University, Palo Alto, California, United States of America.
Andrew R
AR
Hoffman
Eli and Edythe Broad Center for Regenerative Medicine & Stem Cell Research, University of California Los Angeles, Los Angeles, California, United States of America; Department of Molecular and Medical Pharmacology, University of California Los Angeles, Los Angeles, California, United States of America.
James
J
Byrne
Institute for Stem Cell Biology and Regenerative Medicine, Department of Obstetrics and Gynecology and Department of Genetics, Stanford University, Stanford, California, United States of America.
Renee A
RA
Reijo Pera
Institute for Stem Cell Biology and Regenerative Medicine, Department of Obstetrics and Gynecology and Department of Genetics, Stanford University, Stanford, California, United States of America.
Vittorio
V
Sebastiano
D000328:Adult; D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods* / Q000592:standards; D002454:Cell Differentiation; D002460:Cell Line; D065150:Cellular Reprogramming / Q000187:drug effects; D058732:Embryoid Bodies; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D020869:Gene Expression Profiling; D005855:Germ Layers / Q000166:cytology; D049452:Green Fluorescent Proteins / Q000235:genetics; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000637:transplantation; D007231:Infant, Newborn; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors / Q000235:genetics; D008297:Male; D051379:Mice; D016513:Mice, SCID; D008875:Middle Aged; D050814:Octamer Transcription Factor-3 / Q000235:genetics; D061251:Primary Cell Culture; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics; D012333:RNA, Messenger / Q000138:chemical synthesis / Q000302:isolation & purification / Q000494:pharmacology*; D016601:RNA-Binding Proteins / Q000235:genetics; D055748:SOXB1 Transcription Factors / Q000235:genetics; D012867:Skin / Q000166:cytology; D013724:Teratoma / Q000209:etiology / Q000473:pathology; D014162:Transfection
D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors; C516326:LIN28B protein, human; C489427:MYC protein, human; D050814:Octamer Transcription Factor-3; C494173:POU5F1 protein, human; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; C527782:SOX2 protein, human; D055748:SOXB1 Transcription Factors; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins