Cytotoxic effects of three new metabolites from Red Sea marine sponge, Petrosia sp.
Marine sponges represent an affluent source of biogenetically unprecedented array of biologically active compounds. This study revealed the isolation of ten compounds from marine sponge of Petrosia sp. Their chemical structures were determined by using 1D and 2D NMR, UV, IR and MS measurements. A polyoxygenated steroid (3β,7β,9α-trihydroxycholest-5-en (1), a purine-derivative (3,7-dimethyl-2-(methylamino)-3H-purin-6(7H)-one (2) and a sphingolipid (N-((3S,E)-1,3-dihydroxytetracos-4-en-2-yl)stearamide (3) proved to be new compounds. Meanwhile, seven known compounds; (4-10) were also identified. The cytotoxicity of the total extract and the isolated compounds were subjected to cytotoxicity evaluation employing two cancer cell lines; HepG2 and MCF-7. All tested compounds exhibited cytotoxic effect on both cancer cell lines with IC(50) in range of 20-500 μM. The proposed mechanism of cytotoxic activities was examined through its molecular affinity to the DNA. Compound 5 showed the highest affinity to the DNA with IC(50) 30 μg/mL.
Department of Natural Products and Alternative Medicine, Faculty of Pharmacy, King Abdulaziz University, PO Box 80260, Jeddah 21589, Saudi Arabia; Department of Pharmacognosy, Faculty of Pharmacy, Minia University, Minia 61519, Egypt.
Ahmed
A
Abdel-Lateff
Department of Marine Chemistry, Faculty of Marine Sciences, King Abdulaziz University, PO. Box 80207, Jeddah 21589, Saudi Arabia. Electronic address: walied1737@yahoo.com.
Walied M
WM
Alarif
Department of Pharmaceutical Chemistry and Phytochemistry, Faculty of Pharmacy, King Abdulaziz University, PO Box 80260, Jeddah 21589, Saudi Arabia.
Hany Z
HZ
Asfour
Department of Chemistry, Faculty of Science, King Abdulaziz University, PO. Box 80203, Jeddah 21589, Saudi Arabia; Department of Chemistry, Faculty of Science, Dammietta University, New Dammietta, Egypt.
Seif-Eldin N
SE
Ayyad
Department of Pharmaceutical Chemistry and Phytochemistry, Faculty of Pharmacy, King Abdulaziz University, PO Box 80260, Jeddah 21589, Saudi Arabia.
Alaa
A
Khedr
Department of Pharmacognosy, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt.
Farid A
FA
Badria
Department of Marine Chemistry, Faculty of Marine Sciences, King Abdulaziz University, PO. Box 80207, Jeddah 21589, Saudi Arabia.
Sultan S
SS
Al-Lihaibi
D000818:Animals; D003603:Cytotoxins / Q000302:isolation & purification / Q000494:pharmacology*; D004247:DNA / Q000378:metabolism; D056945:Hep G2 Cells; D006801:Humans; D019156:Indian Ocean; D061986:MCF-7 Cells; D008387:Marine Toxins / Q000302:isolation & purification / Q000494:pharmacology*; D011161:Porifera / Q000737:chemistry*
D003603:Cytotoxins; D008387:Marine Toxins; D004247:DNA
Cytotoxicity; HepG2; MFC-7; Sphingolipids; Sponges; Sterols