HLJ1 is a novel biomarker for colorectal carcinoma progression and overall patient survival.
The implication of HLJ1, a member of the heat shock protein-40 chaperone family, in colorectal carcinoma (CRC) remains unclear. The aim of this study was to determine the dynamic changes of HLJ1 in CRC both in vitro and in vivo, and the relationship between its level and the survival rate of CRC patients. Both real-time RT-PCR and Western blot were used to detect the expression of HLJ1 in CRC cells, while the distribution of HLJ1 in CRC and its adjacent normal mucosa tissues from CRC patients was determined with immunohistochemistry. Moreover, MTT and in vitro invasive assays were performed to determine the effect of HLJ1 overexpression on cell proliferation and invasion of CRC cells. The results indicated that in highly metastatic CRC cells, the HLJ1 expression was lower than that in lowly metastatic ones, and that the overexpression of HLJ1 significantly inhibited CRC cell proliferation and invasion in vitro. Interestingly, the HLJ1 expression was significantly down-regulated in CRC or lymphatic metastatic tissues from patient, compared to that in the normal mucosa (P<0.05), and the HLJ1 expression was correlated strongly with lymph metastasis, Dukes' stage, and remote metastasis (P<0.05). Most surprisingly, patients with a higher HLJ1 level had a better overall survival rate, compared to that in patients with lower HLJ1 level (P<0.05). Based on all these findings, we conclude that HLJ1 is a strong tumor suppressor for CRC, and thus the down-regulation of the HLJ1 expression may be used as a biomarker to predict clinical outcome of patients with CRC.
Department of Pathology, The Affiliated Hospital of Luzhou Medical College Zhongshan Road 319, Luzhou, 646000, China.
Yong
Y
Liu
Department of Neurosurgery, The Affiliated Hospital of Luzhou Medical College Taiping Road 25, Luzhou, 646000, China.
Jie
J
Zhou
Department of Pathology, The Affiliated Hospital of Luzhou Medical College Zhongshan Road 319, Luzhou, 646000, China.
Cuiwei
C
Zhang
Department of Hepatobiliary Surgery, The Affiliated Hospital of Luzhou Medical College Taiping Road 25, Luzhou, 646000, China.
Wenguang
W
Fu
Department of Pathology, The Affiliated Hospital of Luzhou Medical College Zhongshan Road 319, Luzhou, 646000, China.
Xiuli
X
Xiao
Department of Pathology, The Affiliated Hospital of Luzhou Medical College Zhongshan Road 319, Luzhou, 646000, China.
Sibei
S
Ruan
Department of Pathology, The Affiliated Hospital of Luzhou Medical College Zhongshan Road 319, Luzhou, 646000, China.
Yuan
Y
Zhang
Department of Pathology, The Affiliated Hospital of Luzhou Medical College Zhongshan Road 319, Luzhou, 646000, China.
Xia
X
Luo
Department of Pathology, The Affiliated Hospital of Luzhou Medical College Zhongshan Road 319, Luzhou, 646000, China.
Mingxi
M
Tang
D000230:Adenocarcinoma / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D014408:Biomarkers, Tumor / Q000032:analysis*; D015153:Blotting, Western; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D018450:Disease Progression; D050956:HSP40 Heat-Shock Proteins / Q000096:biosynthesis*; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D016016:Proportional Hazards Models; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction
D014408:Biomarkers, Tumor; C494650:DNAJB4 protein, human; D050956:HSP40 Heat-Shock Proteins
HLJ1; biomarker; colorectal carcinoma (CRC); metastasis; survival rate