Screening of indigenous oxalate degrading lactic acid bacteria from human faeces and South Indian fermented foods: assessment of probiotic potential.
Lactic acid bacteria (LAB) have the potential to degrade intestinal oxalate and this is increasingly being studied as a promising probiotic solution to manage kidney stone disease. In this study, oxalate degrading LAB were isolated from human faeces and south Indian fermented foods, subsequently assessed for potential probiotic property in vitro and in vivo. Based on preliminary characteristics, 251 out of 673 bacterial isolates were identified as LAB. A total of 17 strains were found to degrade oxalate significantly between 40.38% and 62.90% and were subjected to acid and bile tolerance test. Among them, nine strains exhibited considerable tolerance up to pH 3.0 and at 0.3% bile. These were identified as Lactobacillus fermentum and Lactobacillus salivarius using 16S rDNA sequencing. Three strains, Lactobacillus fermentum TY5, Lactobacillus fermentum AB1, and Lactobacillus salivarius AB11, exhibited good adhesion to HT-29 cells and strong antimicrobial activity. They also conferred resistance to kanamycin, rifampicin, and ampicillin, but were sensitive to chloramphenicol and erythromycin. The faecal recovery rate of these strains was observed as 15.16% (TY5), 6.71% (AB1), and 9.3% (AB11) which indicates the colonization ability. In conclusion, three efficient oxalate degrading LAB were identified and their safety assessments suggest that they may serve as good probiotic candidates for preventing hyperoxaluria.
Department of Biochemistry, Centre for Advanced Studies in Organismal and Functional Genomics, School of Biological Sciences, Madurai Kamaraj University (University with Potential for Excellence), Madurai 625 021, India.
Sivasamy
0000-0001-9938-1381
S
Gomathi
Department of Biochemistry, Centre for Advanced Studies in Organismal and Functional Genomics, School of Biological Sciences, Madurai Kamaraj University (University with Potential for Excellence), Madurai 625 021, India.
Ponnusamy
0000-0002-4165-3118
P
Sasikumar
INSERM-U844, Hopital St. Eloi, Institut des Neuroscience de Montpellier Building, 34091 Montpellier, France.
Kolandaswamy
0000-0002-8077-3673
K
Anbazhagan
Department of Biochemistry, Centre for Advanced Studies in Organismal and Functional Genomics, School of Biological Sciences, Madurai Kamaraj University (University with Potential for Excellence), Madurai 625 021, India.
Sundaresan
0000-0002-2100-5252
S
Sasikumar
Department of Biochemistry, Centre for Advanced Studies in Organismal and Functional Genomics, School of Biological Sciences, Madurai Kamaraj University (University with Potential for Excellence), Madurai 625 021, India.
Murugan
M
Kavitha
Department of Biochemistry, Centre for Advanced Studies in Organismal and Functional Genomics, School of Biological Sciences, Madurai Kamaraj University (University with Potential for Excellence), Madurai 625 021, India.
M S
MS
Selvi
Department of Biochemistry, Centre for Advanced Studies in Organismal and Functional Genomics, School of Biological Sciences, Madurai Kamaraj University (University with Potential for Excellence), Madurai 625 021, India.
Govindan Sadasivam
GS
Selvam
D000328:Adult; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001419:Bacteria / Q000145:classification / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D001422:Bacterial Adhesion; D001646:Bile; D045744:Cell Line, Tumor; D005243:Feces / Q000382:microbiology*; D005285:Fermentation; D005516:Food Microbiology; D005947:Glucose / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007194:India; D007422:Intestines / Q000382:microbiology; D019344:Lactic Acid / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability; D064307:Microbiota; D010070:Oxalates / Q000378:metabolism*; D019936:Probiotics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D051381:Rats; D055815:Young Adult
D000900:Anti-Bacterial Agents; D010070:Oxalates; D012336:RNA, Ribosomal, 16S; D019344:Lactic Acid; D005947:Glucose