[Evaluation of the use of SPEED-OLIGO Mycobacteria for identification of Mycobacterium species].
OBJECTIVE
To evaluate the use of SPEED-OLIGO MYCOBACTERIA (Vircell, Spain) in identifying Mycobacterium species.


METHODS
We examined 15 type or reference strains of mycobacteria (M. tuberculosis H37Rv and 14 non-tuberculosis mycobacteria), 48 clinical isolates, and 17 AFB-positive sputa by using SPEED-OLIGO MYCOBACTERIA, and compared the results with those obtained using other referral methods available for species identification.


RESULTS
SPEED-OLIGO MYCOBACTERIA yielded favorable results in 80.0%, 91.7%, and 88.2% of the cases of the tested type/reference strains, clinical isolates, and clinical samples, respectively. However, the type/reference strains M. celatum, M. fortuitum subsp. fortuitum, and M. marinum, and the clinical isolates M. intermedium, M. marinum, and M. szulgai were misidentified when SPEED-OLIGO MYCOBACTERIA was used.


CONCLUSIONS
SPEED-OLIGO MYCOBACTERIA can facilitate the rapid identification of Mycobacterium species mainly because of its short turn-around time and simple procedures. However, the accuracy of this method remains unsatisfactory.
Kinuyo
K
Chikamatsu
Akio
A
Aono
Hiroyuki
H
Yamada
Tomoko
T
Kato
Satoshi
S
Mitarai
D006801:Humans; D009161:Mycobacterium / Q000302:isolation & purification*; D011933:Reagent Kits, Diagnostic
D011933:Reagent Kits, Diagnostic