Determination of UV active inorganic anions in potable and high salinity water by ion pair reversed phase liquid chromatography.
Reversed phase column was dynamically modified into anion exchange column using various types of tetraalkylammonium salts as ion pair reagents (IPRs) for the separation and quantification of toxic anions such as nitrite, bromate, bromide and nitrate in potable and high salinity water. Various chromatographic parameters such as types and concentration of IPRs, concentration of organic modifier, phosphate buffer and mobile phase pH were optimized for the base-line separation of anions. The lowest detection limits (LDLs) were 0.2 for nitrate and nitrite, 0.6 µg ml(-1)for bromate and bromide respectively for potable water samples. NaCl and Na₂SO₄ were incorporated in the mobile phase for the analysis of high salinity water samples to minimize matrix interferences. This has resulted in change in elution order of anions, better tolerance of matrix anions such as chloride and sulphate. The developed method was successfully utilized for analysis of anions in potable, high salinity and sea water samples.
Chromatography Laboratory, Chemistry Division, PINSTECH, PO Nilore, Islamabad, Pakistan. Electronic address: sadafjabbarshah@hotmail.com.
Sadaf
S
Sadiq Khan
Chromatography Laboratory, Chemistry Division, PINSTECH, PO Nilore, Islamabad, Pakistan.
M
M
Riaz
D000838:Anions; D056148:Chromatography, Reverse-Phase / Q000379:methods; D060766:Drinking Water / Q000032:analysis*; D054712:Salinity; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D014867:Water / Q000032:analysis
D000838:Anions; D060766:Drinking Water; D014867:Water
High salinity water; Ion pair liquid chromatography; Reversed phase column; Tetraalkylammonium salts; Toxic anions