Perilla oil improves blood flow through inhibition of platelet aggregation and thrombus formation.
The inhibitory effects of perilla oil on the platelet aggregation in vitro and thrombosis in vivo were investigated in comparison with aspirin, a well-known blood flow enhancer. Rabbit platelet-rich plasma was incubated with perilla oil and aggregation inducers collagen or thrombin, and the platelet aggregation rate was analyzed. Perilla oil significantly inhibited both the collagen- and thrombin-induced platelet aggregations, in which the thromboxane B2 formation from collagen-activated platelets were reduced in a concentration-dependent manner. Rats were administered once daily by gavage with perilla oil for 1 week, carotid arterial thrombosis was induced by applying 35% FeCl3-soaked filter paper for 10 min, and the blood flow was monitored with a laser Doppler probe. Perilla oil delayed the FeCl3-induced arterial occlusion in a dose-dependent manner, doubling the occlusion time at 0.5 mL/kg. In addition, a high dose (2 mL/kg) of perilla oil greatly prevented the occlusion, comparable to the effect of aspirin (30 mg/kg). The results indicate that perilla oil inhibit platelet aggregation by blocking thromboxane formation, and thereby delay thrombosis following oxidative arterial wall injury. Therefore, it is proposed that perilla oil could be a good candidate without adverse effects for the improvement of blood flow.
College of Veterinary Medicine, Chungbuk National University, Cheongju, Korea.
Ja-Young
JY
Jang
Misuba RTech Co., Ltd., Asan, Korea.
Tae-Su
TS
Kim
College of Veterinary Medicine, Chungbuk National University, Cheongju, Korea.
Jingmei
J
Cai
College of Veterinary Medicine, Chungbuk National University, Cheongju, Korea.
Jihyun
J
Kim
College of Veterinary Medicine, Chungbuk National University, Cheongju, Korea.
Youngeun
Y
Kim
College of Veterinary Medicine, Chungbuk National University, Cheongju, Korea.
Kyungha
K
Shin
College of Veterinary Medicine, Chungbuk National University, Cheongju, Korea.
Kwang-Sei
KS
Kim
Misuba RTech Co., Ltd., Asan, Korea.
Sung-Pyo
SP
Lee
Department of Food Science and Nutrition, Hoseo University, Asan, Korea.
Myung-Hwa
MH
Kang
College of Veterinary Medicine, Chungbuk National University, Cheongju, Korea.
Ehn-Kyoung
EK
Choi
College of Veterinary Medicine, Kyungpook National University, Daegu, Korea.
Man-Hee
MH
Rhee
College of Veterinary Medicine, Chungbuk National University, Cheongju, Korea.
Yun-Bae
YB
Kim
Platelet aggregation; perilla oil; thrombosis; thromboxane B2