Dickeya aquatica sp. nov., isolated from waterways.
Pectinolytic Gram-negative bacteria were isolated from different waterways in the UK and Finland. Three strains (174/2(T), 181/2 and Dw054) had the same 16S rRNA gene sequences which shared 99% sequence similarity to species of the genus Dickeya, and a phylogeny of related genera confirmed attribution to this genus. Fatty acid profile analysis of all three strains found a high proportion of C16 : 1ω7c/C16 : 1ω7c and C16 : 0 fatty acids, and library profile searches found closest matches to Dickeya chrysanthemi. Production of a concatenated phylogeny using six loci, recA, gapA, atpD, gyrB, infB and rpoB, provided a high-resolution phylogeny which placed strains 174/2(T) and 181/2 as a distinct clade, separated from the other species of the genus Dickeya by a relatively long branch-length. DNA-DNA hybridization analysis with a limited number of reference species also supported the distinctiveness of strains 174/2(T) and 181/2 within the genus Dickeya. All three strains could be phenotypically distinguished from other species of the genus by fermentation of melibiose and raffinose but not D-arabinose or mannitol. The name Dickeya aquatica sp. nov. is proposed for the new taxon; the type strain is 174/2(T) ( = NCPPB 4580(T) = LMG 27354(T)).
Food and Environment Research Agency (Fera), Sand Hutton, York, YO41 1LZ, UK.
Neil
N
Parkinson
BCCM/LMG Bacteria Collection Ghent University Laboratory of Microbiology, K. L. Ledeganckstraat 35, B-9000 Ghent, Belgium.
Paul
P
DeVos
Department of Agricultural Sciences, PO Box 27, 00014 University of Helsinki, Finland.
Minna
M
Pirhonen
Food and Environment Research Agency (Fera), Sand Hutton, York, YO41 1LZ, UK.
John
J
Elphinstone
D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics; D004755:Enterobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D005227:Fatty Acids / Q000737:chemistry; D005387:Finland; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D006113:United Kingdom; D014871:Water Microbiology
D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S