Thermodynamic description of peptide adsorption on mixed-mode resins.
In this work the adsorption of tri-peptides on a mixed-mode resin was studied using isocratic pulse response experiments. Various salt concentration, temperature and pH combinations were used to measure retention times of several tri-peptides. The experiments were evaluated according to an extension of the stoichiometric displacement model and the steric mass action model of protein-ligand binding. The application of this model in the understanding of mixed mode adsorption process is discussed. A unique set of meaningful thermodynamic parameters was obtained for each resin-peptide-temperature and resin-peptide-pH combination. Finally it was shown that these thermodynamic parameters can be used in defining quantitative relationships within the framework of extra thermodynamic relationships.
Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands.
Sreekanth
S
Chilamkurthi
Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands.
David Méndez
DM
Sevillano
Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands.
Leonoor H G
LH
Albers
Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands; DSM Biotechnology Center, Alexander Fleminglaan 1, 2613 AX Delft, The Netherlands.
Manas Ranjan
MR
Sahoo
Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands.
Peter J T
PJ
Verheijen
Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands.
Luuk A M
LA
van der Wielen
DSM Biotechnology Center, Alexander Fleminglaan 1, 2613 AX Delft, The Netherlands.
Jeroen L
JL
den Hollander
Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands. Electronic address: m.ottens@tudelft.nl.
Marcel
M
Ottens
D000327:Adsorption; D000837:Anion Exchange Resins / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008956:Models, Chemical; D008970:Molecular Weight; D010455:Peptides / Q000737:chemistry*; D012965:Sodium Chloride / Q000737:chemistry; D013696:Temperature; D013816:Thermodynamics
D000837:Anion Exchange Resins; D010455:Peptides; D012965:Sodium Chloride
Gibbs free energy; Mixed-mode chromatography; Peptide adsorption; Thermodynamic modelling; Tri-peptides