[Down-regulation of Zc3h12d expression in lung tissues of rats with acute lung injury induced by intestinal ischemia-reperfusion].
OBJECTIVE
To investigate the changes in the expression levels of CCCH zinc finger type-containing 12D(Zc3h12d) gene in lung tissues of rats with acute lung injury (ALI) induced by small intestinal ischemia-reperfusion (IIRI).


METHODS
Forty healthy male Sprague-Dawley (SD) rats were equally randomized into sham-operated group (control), 30-minute IIRI group, 60-minute IIRI group, 120-minute IIRI group. Rats in the IIRI groups were subjected to 60 minutes of superior mesenteric artery (SMA) occlusion and to reperfusion for 30, 60 and 120 minutes, respectively. Then the rats were sacrificed. Their lung tissues and sera were obtained, respectively. The lung tissues were examined for wet/dry (W/D) lung mass ratios and pathological changes through HE staining. The levels of TNF-α and IL-1β in sera and lung tissue homogenates were detected by ELISA. The dynamic expression of Zc3h12d mRNA and protein in lung tissues was assayed by real-time quantitative PCR (qRT-PCR), immunohistochemistry and Western blotting.


RESULTS
Compared with the control group, IIRI groups presented the thickening of alveolar wall, the hemorrhage of alveolar space and the increased W/D ratios (P<0.05). qRT-PCR, immunohistochemistry and Western blotting showed that the expression of Zc3h12d mRNA and protein significantly decreased (P<0.05) after 120-minute reperfusion.


CONCLUSIONS
IIRI can down-regulate Zc3h12d gene expression in lung tissue, which implies that Zc3h12d protein may protect rats from IIRI-induced ALI.
Department of Thoracic Surgery, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
Wenchen
W
Wang
Zhipei
Z
Zhang
Bo
B
Yang
Hong
H
Zhang
Zhonghu
Z
Ge
Haitao
H
Liu
Tao
T
Jiang
D055371:Acute Lung Injury / Q000209:etiology* / Q000235:genetics* / Q000473:pathology; D000818:Animals; D015536:Down-Regulation; D004722:Endoribonucleases; D053583:Interleukin-1beta / Q000097:blood / Q000378:metabolism; D007422:Intestines / Q000098:blood supply*; D008168:Lung / Q000378:metabolism* / Q000473:pathology; D008297:Male; D011506:Proteins / Q000235:genetics* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000150:complications*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood / Q000378:metabolism
D053583:Interleukin-1beta; D011506:Proteins; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D004722:Endoribonucleases; C000589517:ZC3H12D protein, rat