Detection of human cytomegalovirus DNA in various blood components after liver transplantation.
The quantification of human cytomegalovirus (HCMV DNA) by real-time PCR is currently a primary option for laboratory diagnosis of HCMV infection. However, the optimal sample material remains controversial due to the use of different PCR assays. To explore the best blood component for HCMV DNA surveillance after liver transplantation, whole blood (WB), serum (SE), and plasma (PL) specimens were collected simultaneously from targeted patients and examined for HCMV DNA using one commercially available assay. The HCMV DNA-positive rate with WB (16.67%) was higher than that with either SE or PL (8.33%, both P<0.01). Quantitative DNA levels in WB were of greater magnitude than those in SE (WB-SE mean log-transformed difference, 0.99; 95%CI=0.74-1.25; P<0.0001) and PL (WB-PL mean log-transformed difference, 1.37; 95%CI=1.07-1.66; P<0.0001). Dynamic monitoring revealed that HCMV DNA in WB was positive sooner and had higher values for a longer period of time during therapy. With earlier positive detection, higher sensitivity, and yield of greater viral loads, WB compared favorably to SE or PL and hence is recommended as the superior material for HCMV DNA surveillance after liver transplantation. In addition, infant recipients require more intensive monitoring and prophylactic care because of their higher susceptibility to primary HCMV infection.
Department of Clinical Laboratory, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
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XY
Chen
Department of Clinical Laboratory, Rushan Hospital, Shandong, China.
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PF
Hou
Department of Clinical Laboratory, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
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J
Bi
Department of Clinical Laboratory, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
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CM
Ying
D000328:Adult; D016009:Chi-Square Distribution; D003587:Cytomegalovirus / Q000235:genetics* / Q000302:isolation & purification; D003586:Cytomegalovirus Infections / Q000097:blood* / Q000175:diagnosis; D004279:DNA, Viral / Q000097:blood*; D005500:Follow-Up Studies; D006801:Humans; D007223:Infant; D057230:Limit of Detection; D008099:Liver / Q000821:virology*; D016031:Liver Transplantation / Q000009:adverse effects*; D008297:Male; D011933:Reagent Kits, Diagnostic; D060888:Real-Time Polymerase Chain Reaction; D012698:Serologic Tests; D019562:Viral Load
D004279:DNA, Viral; D011933:Reagent Kits, Diagnostic