Differential expression of Notch component and effector genes during ovarian follicle and corpus luteum development during the oestrous cycle.
Ovarian dynamics throughout the female oestrous cycle (EC) are characterised by cyclical follicle and corpus luteum (CL) development. These events are tightly regulated, involving extensive cell-to-cell communication. Notch is an evolutionarily well conserved cell-signalling pathway implicated in cell-fate decisions in several tissues. Here, we evaluated the extra-vascular expression patterns of Notch component and effector genes during follicle and CL development throughout the EC. Five mature CD1 female mice were killed at each EC stage. Blood samples were collected for progesterone measurement, ovaries were processed for immunohistochemistry and expression patterns of Notch components (Notch1, 2 and 3, Jagged1 and Delta-like1 and 4) and effectors (Hes1, Hes2 and Hes5) were characterised. Nuclear detection of Notch effectors indicates that Notch signalling is active in the ovary. Notch components and effectors are differentially expressed during follicle and CL development throughout the EC. The spatial and temporal specific expression patterns are associated with follicle growth, selection and ovulation or atresia and CL development and regression.
Reproduction and Obstetrics, CIISA, Faculty of Veterinary Medicine, University of Lisbon, 1300-477 Lisbon, Portugal.
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Murta
Reproduction and Obstetrics, CIISA, Faculty of Veterinary Medicine, University of Lisbon, 1300-477 Lisbon, Portugal.
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M
Batista
Reproduction and Obstetrics, CIISA, Faculty of Veterinary Medicine, University of Lisbon, 1300-477 Lisbon, Portugal.
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Silva
Reproduction and Obstetrics, CIISA, Faculty of Veterinary Medicine, University of Lisbon, 1300-477 Lisbon, Portugal.
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Trindade
Reproduction and Obstetrics, CIISA, Faculty of Veterinary Medicine, University of Lisbon, 1300-477 Lisbon, Portugal.
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Mateus
Reproduction and Obstetrics, CIISA, Faculty of Veterinary Medicine, University of Lisbon, 1300-477 Lisbon, Portugal.
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Duarte
Reproduction and Obstetrics, CIISA, Faculty of Veterinary Medicine, University of Lisbon, 1300-477 Lisbon, Portugal.
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Lopes-da-Costa
D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism; D002135:Calcium-Binding Proteins; D003338:Corpus Luteum / Q000378:metabolism*; D030762:Estrous Cycle / Q000235:genetics* / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism; D000072100:Jagged-1 Protein / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D006080:Ovarian Follicle / Q000378:metabolism*; D010053:Ovary / Q000378:metabolism; D051881:Receptor, Notch1 / Q000235:genetics / Q000378:metabolism; D051883:Receptor, Notch2 / Q000235:genetics / Q000378:metabolism; D000071656:Receptor, Notch3 / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology*; D000072056:Transcription Factor HES-1 / Q000235:genetics / Q000378:metabolism
D048868:Adaptor Proteins, Signal Transducing; D051792:Basic Helix-Loop-Helix Proteins; D002135:Calcium-Binding Proteins; D036341:Intercellular Signaling Peptides and Proteins; D047908:Intracellular Signaling Peptides and Proteins; D000072100:Jagged-1 Protein; D008565:Membrane Proteins; D051881:Receptor, Notch1; D051883:Receptor, Notch2; D000071656:Receptor, Notch3; D012097:Repressor Proteins; D000072056:Transcription Factor HES-1; C519665:DLL4 protein, mouse; C512925:Dlk1 protein, mouse; C489734:Hes1 protein, mouse; C489685:Hes5 protein, mouse; C497762:Notch1 protein, mouse; C497765:Notch2 protein, mouse; C499373:Notch3 protein, mouse