Spatially selecting a single cell for lysis using light-induced electric fields.
An optoelectronic tweezing (OET) device, within an integrated microfluidic channel, is used to precisely select single cells for lysis among dense populations. Cells to be lysed are exposed to higher electrical fields than their neighbours by illuminating a photoconductive film underneath them. Using beam spot sizes as low as 2.5 μm, 100% lysis efficiency is reached in <1 min allowing the targeted lysis of cells.
University of Glasgow, Division of Biomedical Engineering, G12 8LT, Scotland.
Christian
C
Witte
Clemens
C
Kremer
Mayuree
M
Chanasakulniyom
Julien
J
Reboud
Rab
R
Wilson
Jonathan M
JM
Cooper
Steven L
SL
Neale
D018929:Cell Culture Techniques / Q000295:instrumentation; D002458:Cell Fractionation / Q000295:instrumentation*; D002469:Cell Separation / Q000295:instrumentation*; D002478:Cells, Cultured; D004574:Electromagnetic Fields; D004867:Equipment Design; D019544:Equipment Failure Analysis; D004912:Erythrocytes / Q000166:cytology / Q000502:physiology* / Q000528:radiation effects; D006801:Humans; D008027:Light; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D008846:Micromanipulation / Q000295:instrumentation*; D052898:Optical Tweezers
electroporation; lysis; optoelectronic; single cell