Impact of overexpression of Sushi repeat-containing protein X-linked 2 gene on outcomes of gastric cancer.
OBJECTIVE
Sushi repeat-containing protein X-linked 2 (SRPX2) was first described as a downstream target gene for E2A-HLA, which causes pro-B acute leukemia. SRPX2 is considered to promote cellular migration and adhesion in cancers. Our objective was to evaluate the relative expression of the SRPX2 gene and to determine whether such expression correlates with outcomes in patients with gastric cancer.


METHODS
Surgical specimens of cancer tissue and adjacent normal mucosa obtained from 227 patients with previously untreated gastric cancer were examined. SRPX2 mRNA expression levels of cancer tissue and adjacent normal mucosa were measured by quantitative real-time polymerase chain reaction. We evaluated the clinicopathological significance of the relative expression of SRPX2 in patients with gastric cancer.


RESULTS
SRPX2 expression was higher in cancer tissue than in adjacent normal mucosa (P < 0.001). On analysis of the relations between gene expression and clinicopathological factors, SRPX2 expression correlated with tumor size and distant metastasis. Overall survival was significantly lower in patients whose tumors had high SRPX2 expression than in those who had low SRPX2 expression (P = 0.003). Multivariate analysis showed that high SRPX2 expression was an independent predictor of survival (HR = 2.028, 95% CI = 1.265-3.251).


CONCLUSIONS
SRPX2 expression was significantly higher in gastric cancer tissue than in adjacent normal mucosa, and overexpression of the SRPX2 gene is considered a useful independent predictor of outcomes in patients with gastric cancer.
Gastroenterological Center, Yokohama City University Medical Center, Yokohama-shi, Kanagawa-ken, Japan; Department of Surgery, Yokohama City University, Yokohama-shi, Kanagawa-ken, Japan.
Takanobu
T
Yamada
Takashi
T
Oshima
Kazue
K
Yoshihara
Tsutomu
T
Sato
Akito
A
Nozaki
Manabu
M
Shiozawa
Mitsuyoshi
M
Ota
Takaki
T
Yoshikawa
Makoto
M
Akaike
Kazushi
K
Numata
Yasushi
Y
Rino
Chikara
C
Kunisaki
Katsuaki
K
Tanaka
Toshio
T
Imada
Munetaka
M
Masuda
D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005500:Follow-Up Studies; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D009367:Neoplasm Staging; D009419:Nerve Tissue Proteins / Q000235:genetics*; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology; D015996:Survival Rate; D014407:Tumor Cells, Cultured
D014408:Biomarkers, Tumor; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; C116881:SRPX2 protein, human
SRPX2; gastric cancer; prognosis