Serum paraoxonase activity and oxidative stress and their relationship with obesity-related metabolic syndrome and non-alcoholic fatty liver disease in obese children and adolescents.
OBJECTIVE
Oxidative stress has been reported to be involved in the pathogenesis of metabolic disorders related with obesity. The aim of the study was to investigate the association of oxidative stress and paraoxonase activities with non-alcoholic fatty liver disease (NAFLD) as well as metabolic syndrome.


METHODS
A total of 109 obese children and adolescents and 44 healthy and lean control subjects were enrolled in the study. According to their ultrasonographic steatosis scores, they were classified into four groups as follows: healthy children; obese, non-NAFLD; obese, grade I-NAFLD; and obese, grade II-III NAFLD. The biochemical parameters and insulin resistance (HOMA-IR) were evaluated from fasting samples. The plasma total antioxidant status (TAS), total oxidant status (TOS), and serum paraoxonase activities were measured and then oxidative stress index (OSI) was calculated as the indicator of degree of oxidative stress.


RESULTS
As the steatosis increased, the alanine aminotransferase, C-reactive protein, HOMA-IR, total cholesterol, and LDL cholesterol increased, whereas HDL cholesterol decreased. The TAS measurements were higher in the obese NAFLD group compared with that of the healthy control group. The TOS and OSI measurements did not differ between the groups. Paraoxonase activities increased significantly as steatosis increased.


CONCLUSIONS
Among the children in this study, no relationship could be demonstrated between obesity with/without steatosis and oxidant/antioxidant status.
Emel
E
Torun
Selim
S
Gökçe
İlker Tolga
İT
Ozgen
Sinem
S
Aydın
Yasar
Y
Cesur
D000293:Adolescent; D000975:Antioxidants / Q000378:metabolism*; D043303:Aryldialkylphosphatase / Q000097:blood*; D002648:Child; D005260:Female; D006801:Humans; D007333:Insulin Resistance; D008297:Male; D024821:Metabolic Syndrome / Q000097:blood* / Q000209:etiology; D065626:Non-alcoholic Fatty Liver Disease / Q000097:blood* / Q000209:etiology; D018384:Oxidative Stress / Q000502:physiology*; D063766:Pediatric Obesity / Q000097:blood* / Q000150:complications
D000975:Antioxidants; D043303:Aryldialkylphosphatase