Structure and gene cluster of the O-antigen of Escherichia coli O36.
The O-polysaccharide (O-antigen) of Escherichia coli O36 was isolated from the lipopolysaccharide and studied by sugar analyses and Smith degradation along with (1)H and (13)C NMR spectroscopy. The following structure of the branched pentasaccharide repeating unit was established, which is unique among the known structures of bacterial polysaccharides: The O-antigen gene cluster of E. coli O36 has been sequenced. The gene functions were tentatively assigned by comparison with sequences in the available databases and found to be in full agreement with the O-polysaccharide structure.
N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation. Electronic address: perepel@ioc.ac.ru.
Andrei V
AV
Perepelov
TEDA School of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin, China.
Quan
Q
Wang
N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation; Higher Chemical College, Russian Academy of Sciences, 125047 Moscow, Russian Federation.
Evgeniya A
EA
Levina
N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
Olga G
OG
Ovchinnikova
TEDA School of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin, China.
Ye
Y
Qian
N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
Alexander S
AS
Shashkov
TEDA School of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin, China.
Lei
L
Wang
N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
Yuriy A
YA
Knirel
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D004926:Escherichia coli / Q000235:genetics*; D008745:Methylation; D008969:Molecular Sequence Data; D005810:Multigene Family; D019906:Nuclear Magnetic Resonance, Biomolecular; D019081:O Antigens / Q000737:chemistry* / Q000235:genetics; D016366:Open Reading Frames
D019081:O Antigens
Bacterial polysaccharide structure; Escherichia coli; Lipopolysaccharide; O-antigen; O-antigen gene cluster