Monitoring bone morphogenetic protein-2 and -7, soluble receptor activator of nuclear factor-κB ligand and osteoprotegerin levels in the peri-implant sulcular fluid during the osseointegration of hydrophilic-modified sandblasted acid-etched and sandblasted acid-etched surface dental implants.
OBJECTIVE
The implant surface plays a major role in the biological response to titanium dental implants. The aim of this study was to investigate levels of soluble receptor activator of nuclear factor-κB ligand (sRANKL), osteoprotegerin (OPG), bone morphogenetic protein-2 (BMP-2) and -7 (BMP-7) in the peri-implant crevicular fluid (PICF) of different implants during the osseointegration period.


METHODS
Forty-seven patients (22 females and 25 males, mean age 47.34 ± 10.11) were included in this study. Forty-seven implants from two implant systems (group A1 (sandblasted acid-etched [SLA]-16), group A2 (hydrophilic-modified SLA [SLActive]-16), and group B (sandblasted acid-etched [SLA]-15) were placed using standard surgical protocols. PICF samples, plaque index, gingival index and probing depth measurements were obtained at 1 and 3 mo after surgery. PICF levels of sRANKL, OPG, BMP-2/-7 were analyzed by ELISA.


RESULTS
No complications were observed during the healing period. No significant differences were observed in the PICF levels of sRANKL, OPG, BMP-2 and BMP-7 for all groups at any time point (p > 0.05). A significant decrease was observed in BMP-2 levels in group A1 (p < 0.05). A significant increase in BMP-7 levels was observed only for group A2 (p < 0.05). There was a strong negative correlation between OPG and gingival index and a negative correlation between BMP-7 and plaque index (p < 0.05).


CONCLUSIONS
Considering the correlations between clinical and biochemical parameters, the levels of these cytokines in PICF during early healing of implants reflects the degree of peri-implant inflammation, rather than differences in the implant surfaces.
Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Selçuk University, Konya, Turkey.
D
D
Dolanmaz
M
M
Saglam
O
O
Inan
N
N
Dundar
G
G
Alniacık
B
B
Gursoy Trak
E
E
Kocak
S S
SS
Hakki
D000134:Acid Etching, Dental / Q000379:methods; D000328:Adult; D055396:Bone Morphogenetic Protein 2 / Q000032:analysis*; D055419:Bone Morphogenetic Protein 7 / Q000032:analysis*; D045662:Dental Etching / Q000379:methods; D015921:Dental Implants; D003774:Dental Plaque Index; D017267:Dental Prosthesis Design; D005260:Female; D005500:Follow-Up Studies; D005883:Gingival Crevicular Fluid / Q000737:chemistry*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008297:Male; D008875:Middle Aged; D016348:Osseointegration / Q000502:physiology*; D053244:Osteoprotegerin / Q000032:analysis*; D010512:Periodontal Index; D010514:Periodontal Pocket / Q000145:classification; D053245:RANK Ligand / Q000032:analysis*; D013499:Surface Properties
C525718:BMP2 protein, human; C525819:BMP7 protein, human; D055396:Bone Morphogenetic Protein 2; D055419:Bone Morphogenetic Protein 7; D015921:Dental Implants; D053244:Osteoprotegerin; D053245:RANK Ligand; C505688:TNFSF11 protein, human
bone morphogenetic proteins; dental implant; osteoprotegerin; receptor activator of nuclear factor-kappa B