First isolation and molecular characterization of foot-and-mouth disease virus in Benin.
Foot-and-mouth disease (FMD) is a highly contagious viral disease of cloven-hoofed animals. It is one of the most economically devastating diseases affecting livestock animals. In West Africa, where constant circulation of FMD virus (FMDV) is assumed, very few studies on the characterization of circulating strains have been published. This study describes the first isolation and characterization of FMDV in Benin. FMDV was isolated from 42 samples. Antigen Capture Elisa (Ag-ELISA) and VP1 coding sequence analysis revealed 33 strains of serotype O and 9 strains of serotype A. Phylogenetic analysis of the VP1 sequence revealed two different groups of type O isolates and one group of A isolates. VP1 sequence comparison with the sequences available in the GenBank database revealed a close relationship of the Benin isolates with topotype O of West Africa and with African topotype A of genotype VI. Knowledge of the recent strains circulating in Benin should contribute to better selection of vaccine strains and enable the updating of molecular epidemiology data available for West Africa in general.
ANSES, Laboratory for Animal Health, UMR1161 (ANSES, Inra, Enva), 23 Avenue du Général de Gaulle, 94706 Maisons-Alfort, France.
Kamila
K
Gorna
Laboratoire de Recherche en Biologie Appliquée, Ecole Polytechnique d'Abomey-Calavi, Université d'Abomey-Calavi, 01 BP 2009 Cotonou, Benin; Département de Santé Animale, Institut de Médecine Tropicale, Nationalestraat, 155, Anvers, Belgium; Research Unit of Epidemiology and Risk Analysis applied to veterinary science (UREAR-ULg), Fundamental and Applied Research for Animals & Health, Faculty of Veterinary Medicine, University of Liege, Boulevard de Colonster, 20, B42, 4000 Liege, Belgium.
Evelyne
E
Houndjè
ANSES, Laboratory for Animal Health, UMR1161 (ANSES, Inra, Enva), 23 Avenue du Général de Gaulle, 94706 Maisons-Alfort, France.
Aurore
A
Romey
ANSES, Laboratory for Animal Health, UMR1161 (ANSES, Inra, Enva), 23 Avenue du Général de Gaulle, 94706 Maisons-Alfort, France.
Anthony
A
Relmy
ANSES, Laboratory for Animal Health, UMR1161 (ANSES, Inra, Enva), 23 Avenue du Général de Gaulle, 94706 Maisons-Alfort, France.
Sandra
S
Blaise-Boisseau
Laboratoire de Recherche en Biologie Appliquée, Ecole Polytechnique d'Abomey-Calavi, Université d'Abomey-Calavi, 01 BP 2009 Cotonou, Benin; Département de Production et Santé Animales, Ecole Polytechnique d'Abomey-Calavi, Université d'Abomey-Calavi, 01 BP 2009 Cotonou, Benin.
Marc
M
Kpodékon
Research Unit of Epidemiology and Risk Analysis applied to veterinary science (UREAR-ULg), Fundamental and Applied Research for Animals & Health, Faculty of Veterinary Medicine, University of Liege, Boulevard de Colonster, 20, B42, 4000 Liege, Belgium.
Claude
C
Saegerman
ANSES, Laboratory for Animal Health, UMR1161 (ANSES, Inra, Enva), 23 Avenue du Général de Gaulle, 94706 Maisons-Alfort, France.
François
F
Moutou
ANSES, Laboratory for Animal Health, UMR1161 (ANSES, Inra, Enva), 23 Avenue du Général de Gaulle, 94706 Maisons-Alfort, France.
Stephan
S
Zientara
ANSES, Laboratory for Animal Health, UMR1161 (ANSES, Inra, Enva), 23 Avenue du Général de Gaulle, 94706 Maisons-Alfort, France. Electronic address: Labib.bakkali-kassimi@anses.fr.
Labib
L
Bakkali Kassimi
D000354:Africa, Western; D000595:Amino Acid Sequence; D000818:Animals; D001541:Benin; D036022:Capsid Proteins / Q000235:genetics; D002417:Cattle; D002418:Cattle Diseases / Q000821:virology*; D005536:Foot-and-Mouth Disease / Q000821:virology*; D029544:Foot-and-Mouth Disease Virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D005838:Genotype; D008969:Molecular Sequence Data; D010802:Phylogeny; D012703:Serotyping
D036022:Capsid Proteins; C442501:VP1 protein, Foot-and-mouth disease virus
Benin; Foot-and-mouth disease; Virus isolation; West Africa