Cross-generational trans fat intake exacerbates UV radiation-induced damage in rat skin.
We evaluated the influence of dietary fats on ultraviolet radiation (UVR)-induced oxidative damage in skin of rats. Animals from two consecutive generations born of dams supplemented with fats during pregnancy and breastfeeding were maintained in the same supplementation: soybean-oil (SO, rich in n-6 FA, control group), fish-oil (FO, rich in n-3 FA) or hydrogenated-vegetable-fat (HVF, rich in TFA). At 90 days of age, half the animals from the 2nd generation were exposed to UVR (0.25 J/cm(2)) 3×/week for 12 weeks. The FO group presented higher incorporation of n-3 FA in dorsal skin, while the HVF group incorporated TFA. Biochemical changes per se were observed in skin of the HVF group: greater generation of reactive oxygen species (ROS), lower mitochondrial integrity and increased Na(+)K(+)-ATPase activity. UVR exposure increased skin wrinkles scores and ROS generation and decreased mitochondrial integrity and reduced-glutathione levels in the HVF group. In FO, UVR exposure was associated with smaller skin thickness and reduced levels of protein-carbonyl, together with increased catalase activity and preserved Na(+)K(+)-ATPase function. In conclusion, while FO may be protective, trans fat may be harmful to skin health by making it more vulnerable to UVR injury and thus more prone to develop photoaging and skin cancer.
Programa de Pós-Graduação em Farmacologia, Universidade Federal de Santa Maria (UFSM), RS, Brazil.
R C S
RC
Barcelos
Departamento de Fisiologia e Farmacologia, UFSM, RS, Brazil.
L T
LT
Vey
Departamento de Fisiologia e Farmacologia, UFSM, RS, Brazil.
H J
HJ
Segat
Departamento de Fisiologia e Farmacologia, UFSM, RS, Brazil.
K
K
Roversi
Departamento de Fisiologia e Farmacologia, UFSM, RS, Brazil.
Kr
K
Roversi
Departamento de Fisiologia e Farmacologia, UFSM, RS, Brazil.
V T
VT
Dias
Programa de Pós-Graduação em Farmacologia, Universidade Federal de Santa Maria (UFSM), RS, Brazil.
F
F
Trevizol
Programa de Pós-Graduação em Farmacologia, Universidade Federal de Santa Maria (UFSM), RS, Brazil.
F T
FT
Kuhn
Programa de Pós-Graduação em Farmacologia, Universidade Federal de Santa Maria (UFSM), RS, Brazil.
G S
GS
Dolci
Programa de Pós-Graduação em Farmacologia, Universidade Federal de Santa Maria (UFSM), RS, Brazil.
C S
CS
Pase
Departamento de Tecnologia dos Alimentos, Programa de Pós-Graduação em Ciência Tecnologia dos Alimentos, UFSM, RS, Brazil.
J
J
Piccolo
Departamento de Tecnologia dos Alimentos, Programa de Pós-Graduação em Ciência Tecnologia dos Alimentos, UFSM, RS, Brazil.
J C
JC
Veit
Programa de Pós-Graduação em Farmacologia, Universidade Federal de Santa Maria (UFSM), RS, Brazil; Departamento de Tecnologia dos Alimentos, Programa de Pós-Graduação em Ciência Tecnologia dos Alimentos, UFSM, RS, Brazil.
T
T
Emanuelli
Departamento de Patologia, UFSM, RS, Brazil.
S C A
SC
Luz
Programa de Pós-Graduação em Farmacologia, Universidade Federal de Santa Maria (UFSM), RS, Brazil; Departamento de Fisiologia e Farmacologia, UFSM, RS, Brazil. Electronic address: mariliseeb@yahoo.com.br.
M E
ME
Bürger
D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D004041:Dietary Fats / Q000494:pharmacology; D005227:Fatty Acids / Q000032:analysis; D005260:Female; D005395:Fish Oils / Q000494:pharmacology*; D006865:Hydrogenation; D008928:Mitochondria / Q000187:drug effects / Q000528:radiation effects; D011247:Pregnancy; D050050:Protein Carbonylation / Q000187:drug effects / Q000528:radiation effects; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D012867:Skin / Q000187:drug effects / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013024:Soybean Oil / Q000494:pharmacology; D013472:Sunlight / Q000009:adverse effects; D044242:Trans Fatty Acids / Q000494:pharmacology*; D014466:Ultraviolet Rays / Q000009:adverse effects*
D000975:Antioxidants; D004041:Dietary Fats; D005227:Fatty Acids; D005395:Fish Oils; D017382:Reactive Oxygen Species; D044242:Trans Fatty Acids; D013024:Soybean Oil; D000254:Sodium-Potassium-Exchanging ATPase
Fatty acids; Oxidative stress; Skin; Trans fat; Trans fatty acids; Ultraviolet radiation