Fermented dairy products modulate Citrobacter rodentium-induced colonic hyperplasia.
We evaluated the protective effects of fermented dairy products (FDPs) in an infection model, using the mouse pathogen Citrobacter rodentium (CR). Treatment of mice with FDP formulas A, B, and C or a control product did not affect CR colonization, organ specificity, or attaching and effacing lesion formation. Fermented dairy product A (FDP-A), but neither the supernatant from FDP-A nor β-irradiated (IR) FDP-A, caused a significant reduction in colonic crypt hyperplasia and CR-associated pathology. Profiling the gut microbiota revealed that IR-FDP-A promoted higher levels of phylotypes belonging to Alcaligenaceae and a decrease in Lachnospiraceae (Ruminococcus) during CR infection. Conversely, FDP-A prevented a decrease in Ruminococcus and increased Turicibacteraceae (Turicibacter). Importantly, loss of Ruminococcus and Turicibacter has been associated with susceptibility to dextran sodium sulfate-induced colitis. Our results demonstrate that viable bacteria in FDP-A reduced CR-induced colonic crypt hyperplasia and prevented the loss of key bacterial genera that may contribute to disease pathology.
MRC Centre for Molecular Bacteriology and Infection, Department of Life Sciences, Imperial College London, United Kingdom.
James W
JW
Collins
Danone Nutricia Research, Centre Daniel Carasso, Palaiseau.
Christian
C
Chervaux
MRC Centre for Molecular Bacteriology and Infection, Department of Life Sciences, Imperial College London, United Kingdom.
Benoit
B
Raymond
Danone Nutricia Research, Centre Daniel Carasso, Palaiseau.
Muriel
M
Derrien
C2R, Paris, France.
Rémi
R
Brazeilles
MRC Centre for Molecular Bacteriology and Infection, Department of Life Sciences, Imperial College London, United Kingdom.
Artemis
A
Kosta
Danone Nutricia Research, Centre Daniel Carasso, Palaiseau.
Isabelle
I
Chambaud
MRC Centre for Molecular Bacteriology and Infection, Department of Life Sciences, Imperial College London, United Kingdom.
Valerie F
VF
Crepin
MRC Centre for Molecular Bacteriology and Infection, Department of Life Sciences, Imperial College London, United Kingdom.
Gad
G
Frankel
D000818:Animals; D058448:Biota; D044082:Citrobacter rodentium / Q000254:growth & development*; D003106:Colon / Q000382:microbiology / Q000473:pathology*; D003611:Dairy Products; D004032:Diet / Q000379:methods*; D004756:Enterobacteriaceae Infections / Q000382:microbiology / Q000473:pathology / Q000517:prevention & control*; D005260:Female; D006965:Hyperplasia / Q000473:pathology / Q000517:prevention & control*; D008810:Mice, Inbred C57BL
C. rodentium; DLIT-µCT; bioluminescence imaging; fermented dairy products; microbiota; probiotic