Abnormal expression of CD66a promotes proliferation and inhibits apoptosis of human leukemic B cells in vitro.
The aberrant expression of myeloid antigen CD66 on acute B-lymphoblastic leukemia (B-ALL) cells is a well-documented phenomenon. CD66a is a major subtype of the CD66 family which plays a dual role in different cancers, and the contradictory effect may depend on the isoform ratio of CD66a-4L to CD66a-4S. Although the abnormal expression of CD66a on leukemic B-cells has been reported widely, little is known about the biological function of this aberrant expression. In this study, we showed that inhibition of CD66a in human B-ALL cell lines reduced the cellular proliferative rate and increased the percentage of cellular apoptosis, and the ratio of CD66a-4L to CD66a-4S in leukemic B cells is much higher than that in granulocytes. In addition, alteration of the L:S ratio by silencing and overexpressing the L isoform in B-ALL cell lines confirmed that a high L:S ratio of CD66a in leukemic B cells promotes proliferation and inhibits FasL-induced apoptosis.
Center of Laboratory Technology, China Medical University , Shenyang, Liaoning , China.
Wenjin
W
Zhao
Yan
Y
Zhang
Dandan
D
Liu
Liansheng
L
Zhong
Qun
Q
He
Yujie
Y
Zhao
D015703:Antigens, CD / Q000235:genetics* / Q000378:metabolism; D017209:Apoptosis / Q000235:genetics*; D020169:Caspases / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism; D049109:Cell Proliferation; D053222:Fas Ligand Protein / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006098:Granulocytes / Q000378:metabolism; D006801:Humans; D015448:Leukemia, B-Cell / Q000235:genetics* / Q000378:metabolism; D020033:Protein Isoforms; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics
D015703:Antigens, CD; C069847:CD66 antigens; D015815:Cell Adhesion Molecules; D053222:Fas Ligand Protein; D020033:Protein Isoforms; D034741:RNA, Small Interfering; D020169:Caspases
CD66a; acute leukemic B cells; apoptosis; proliferation