Surface plasmon resonance-induced photoactivation of gold nanoparticles as bactericidal agents against methicillin-resistant Staphylococcus aureus.
Systemic infections caused by methicillin-resistant Staphylococcus aureus (MRSA) and other bacteria are responsible for millions of deaths worldwide, and much of this mortality is due to the rise of antibiotic-resistant organisms as a result of natural selection. Gold nanoparticles synthesized using the standard wet chemical procedure were photoexcited using an 808 nm 2 W laser diode and further administered to MRSA bacteria. Flow cytometry, transmission electron microscopy, contrast phase microscopy, and fluorescence microscopy combined with immunochemical staining were used to examine the interaction of the photoexcited gold nano-particles with MRSA bacteria. We show here that phonon-phonon interactions following laser photoexcitation of gold nanoparticles exhibit increased MRSA necrotic rates at low concentrations and short incubation times compared with MRSA treated with gold nanoparticles alone. These unique data may represent a step forward in the study of bactericidal effects of various nanomaterials, with applications in biology and medicine.
3rd Surgery Clinic, Department of Nanomedicine, "Iuliu Hatieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania.
Lucian
L
Mocan
Department of Endocrinology, Department of Nanomedicine, "Iuliu Hatieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania.
Ioana
I
Ilie
3rd Surgery Clinic, Department of Nanomedicine, "Iuliu Hatieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania.
Cristian
C
Matea
3rd Surgery Clinic, Department of Nanomedicine, "Iuliu Hatieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania.
Flaviu
F
Tabaran
3rd Surgery Clinic, Department of Nanomedicine, "Iuliu Hatieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania.
Ersjebet
E
Kalman
3rd Surgery Clinic, Department of Nanomedicine, "Iuliu Hatieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania.
Cornel
C
Iancu
Department of Physiology, Department of Nanomedicine, "Iuliu Hatieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania.
Teodora
T
Mocan
D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000528:radiation effects; D002470:Cell Survival / Q000187:drug effects; D006046:Gold / Q000008:administration & dosage* / Q000528:radiation effects; D008027:Light; D053768:Metal Nanoparticles / Q000008:administration & dosage* / Q000528:radiation effects; D016106:Methicillin Resistance / Q000528:radiation effects*; D010789:Phototherapy / Q000379:methods*; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000528:radiation effects*; D020349:Surface Plasmon Resonance / Q000379:methods*
D000900:Anti-Bacterial Agents; D006046:Gold
MRSA; SPR; gold nanoparticles; infection; laser; multi-drug resistant bacteria