Live cell imaging of the intracellular compartmentalization of the contaminate benzo[a]pyrene.
This study investigates the cellular response of murine hepatoma cells to the polycyclic aromatic hydrocarbon benzo[a]pyrene (B[a]P) using two-photon and confocal laser scanning microscopy. The intracellular distribution of B[a]P and the B[a]P/AhR complex was visualized time- and concentration-dependent for up to 48 h of exposure. B[a]P was predominantly found in lipid droplets, endoplasmic reticulum and lysosomes, where B[a]P is collected and forms large aggregates. Changes in mitochondrial membrane potential and bleb formation due to high B[a]P concentrations were observed. The imaging data presented in this study provide new insights into the systemic cellular regulation following B[a]P exposure.
Max Bergmann Center of Biomaterials and Institute of Materials Science, Technische Universität Dresden, Budapester Str. 27, 01069 Dresden, Germany. rizwanali79@gmail.com.
Rizwan
R
Ali
Department of Environmental Immunology, Helmholtz Centre for Environmental Research, Leipzig, Germany.
Saskia
S
Trump
Department of Environmental Immunology, Helmholtz Centre for Environmental Research, Leipzig, Germany.
Irina
I
Lehmann
Max Bergmann Center of Biomaterials and Institute of Materials Science, Technische Universität Dresden, Budapester Str. 27, 01069 Dresden, Germany.
Thomas
T
Hanke
D000818:Animals; D001564:Benzo(a)pyrene / Q000378:metabolism* / Q000633:toxicity; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000187:drug effects; D002470:Cell Survival; D004785:Environmental Pollutants / Q000378:metabolism* / Q000633:toxicity; D042541:Intracellular Space / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D018613:Microscopy, Confocal; D057054:Molecular Imaging; D013997:Time Factors
D004785:Environmental Pollutants; D001564:Benzo(a)pyrene
2-Photon; Benzo[a]pyrene; Confocal microscopy; Live cell imaging