Reduced growth and proliferation dynamics of nasal epithelial stem/progenitor cells in nasal polyps in vitro.
Basal cells in nasal epithelium have stemness/progenitor characters and play essential roles in the epithelial remodeling in nasal polyps (NP). We investigate whether the human nasal epithelial stem/progenitor cells (hNESPCs) from patients with NP are inherently distinct from those obtained from healthy controls. Epithelial basal cells were isolated and cultured for four passages from NP tissues and control nasal mucosa. hNESPCs from controls were stained positively with stem cell marker p63 and KRT5 and presented a consistent high Ki67 expression level over four passages. In contrast, hNESPCs from NP patients showed: i). a reduced growth and proliferation rate at each passage by evaluating colony-forming efficiency and doubling time; ii). a lower percentage of Ki67(+) cells among p63(+) cells in the colonies in late passages, which was also confirmed by immunostaining in the NP tissues. Thus reduced growth/proliferation dynamics in hNESPCs from NP could be an important pathological phenomenon in NP development.
1] Department of Otolaryngology, Qilu Hospital, Shandong University, China [2] Department of Otolaryngology, National University of Singapore, National University Health System, Singapore [3].
Xue Min
XM
Yu
1] Department of Otolaryngology, National University of Singapore, National University Health System, Singapore [2].
Chun Wei
CW
Li
Department of Otolaryngology, National University of Singapore, National University Health System, Singapore.
Siew Shuen
SS
Chao
Department of Otolaryngology, National University of Singapore, National University Health System, Singapore.
Ying Ying
YY
Li
Department of Otolaryngology, National University of Singapore, National University Health System, Singapore.
Yan
Y
Yan
1] Department of Otolaryngology, Qilu Hospital, Shandong University, China [2] Department of General Health Care II, Shandong Provincial Hospital affiliated to Shandong University, China.
Xue Ning
XN
Zhao
Department of Otolaryngology, National University of Singapore, National University Health System, Singapore.
Feng Gang
FG
Yu
Department of Otolaryngology, National University of Singapore, National University Health System, Singapore.
Jing
J
Liu
Biostatistics Unit, National University of Singapore, National University Health System, Singapore.
Liang
L
Shen
Department of Otolaryngology, Qilu Hospital, Shandong University, China.
Xin Liang
XL
Pan
Department of Otolaryngology, Qilu Hospital, Shandong University, China.
Li
L
Shi
Department of Otolaryngology, National University of Singapore, National University Health System, Singapore.
De Yun
de Y
Wang
D000328:Adult; D053687:Adult Stem Cells / Q000166:cytology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology; D006801:Humans; D053555:Keratin-5 / Q000096:biosynthesis; D019394:Ki-67 Antigen / Q000096:biosynthesis* / Q000235:genetics; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics; D008875:Middle Aged; D009297:Nasal Mucosa / Q000166:cytology*; D009298:Nasal Polyps / Q000378:metabolism / Q000473:pathology*; D012333:RNA, Messenger / Q000096:biosynthesis
C081876:CKAP4 protein, human; C503940:KRT5 protein, human; D053555:Keratin-5; D019394:Ki-67 Antigen; D008565:Membrane Proteins; D012333:RNA, Messenger