Inhibition of the electron transport strongly affects transcription and transcript levels in Arabidopsis mitochondria.
Mitochondrial transcription rate and RNA steady-state levels were examined in shoots of Arabidopsis seedlings. The shoots were treated with inhibitors of complex III and IV of the cytochrome pathway (CP) and with an inhibitor of the alternative oxidase (AOX) of the mitochondrial electron transport chain. The inhibition of AOX and CP complexes III and IV affected transcription and transcript levels in different ways. CP and AOX inhibitors had opposite effects. Our data support the idea that the redox state of the electron transport chain is involved in the regulation of mitochondrial gene expression at transcriptional and post-transcriptional levels.
Institute of Biology-Genetics, Humboldt University, Chaussestr. 117, 10115 Berlin, Germany.
Yan O
YO
Zubo
Institute of Biology-Genetics, Humboldt University, Chaussestr. 117, 10115 Berlin, Germany; The Siberian Institute of Plant Physiology and Biochemistry SB RAS, Lermontova St., 132, Irkutsk 664033, Russia.
Tatyana V
TV
Potapova
Institute of Biology-Genetics, Humboldt University, Chaussestr. 117, 10115 Berlin, Germany.
Maria V
MV
Yamburenko
The Siberian Institute of Plant Physiology and Biochemistry SB RAS, Lermontova St., 132, Irkutsk 664033, Russia.
Vladislav I
VI
Tarasenko
Institute of Biology-Genetics, Humboldt University, Chaussestr. 117, 10115 Berlin, Germany; The Siberian Institute of Plant Physiology and Biochemistry SB RAS, Lermontova St., 132, Irkutsk 664033, Russia; The Irkutsk State University, Sukhe-Batar St., 5, Irkutsk 664033, Russia.
Yuri M
YM
Konstantinov
Institute of Biology-Genetics, Humboldt University, Chaussestr. 117, 10115 Berlin, Germany. Electronic address: thomas.boerner@rz.hu-berlin.de.
Thomas
T
Börner
D017360:Arabidopsis / Q000187:drug effects* / Q000378:metabolism*; D004579:Electron Transport / Q000187:drug effects*; D004791:Enzyme Inhibitors / Q000378:metabolism; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism*; D010084:Oxidation-Reduction; D018520:Plant Shoots / Q000187:drug effects / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis*; D000077278:RNA, Mitochondrial; D036226:Seedlings / Q000187:drug effects / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects*
D004791:Enzyme Inhibitors; D012333:RNA, Messenger; D000077278:RNA, Mitochondrial; D012313:RNA
Alternative oxidase; Electron transport chain; Mitochondrial RNA; Mitochondrial gene expression; Redox regulation; Retrograde signaling