MicroRNA let-7a-3 gene methylation is associated with karyotyping, CEBPA promoter methylation, and survival in acute myeloid leukemia.
Let-7a-3 transcribes the miRNA let-7a, of which the expression is dysregulated in cancer. We evaluated the significance of let-7a-3 gene methylation in patients with de novo acute myeloid leukemia (AML). Let-7a-3 was methylated in 81.1% (73/90), partially methylated in 12.2% (11/90), or unmethylated in 6.7% (6/90) of patients. Let-7a-3 methylation correlated with AML karyotyping and CCAAT/enhancer binding protein α (CEBPA) methylation. Kaplan-Meier survival analysis predicted that let-7a-3 hypermethylation correlated with better survival in AML with hypomethylated CEBPA or with hypomethylated CEBPA without the favorable karyotype. We conclude that let-7a-3 methylation is a positive prognosticator for AML patients with hypomethylated CEBPA.
Department of Medical Imaging and Radiological Technology, Yuanpei University, Hsinchu, Taiwan.
Ya-Chen
YC
Ko
Department of Clinical Laboratory Sciences and Medical Biotechnology, National Taiwan University, Taipei, Taiwan; Department of Laboratory Medicine and Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan.
Woei-Horng
WH
Fang
Department of Clinical Laboratory Sciences and Medical Biotechnology, National Taiwan University, Taipei, Taiwan.
Tsung-Chin
TC
Lin
Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan.
Hsin-An
HA
Hou
Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan.
Chien-Yuan
CY
Chen
Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan.
Hwei-Fang
HF
Tien
Department of Clinical Laboratory Sciences and Medical Biotechnology, National Taiwan University, Taipei, Taiwan; Department of Laboratory Medicine and Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan. Electronic address: lilin@ntu.edu.tw.
Liang-In
LI
Lin
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D022762:CCAAT-Enhancer-Binding Proteins / Q000235:genetics*; D045744:Cell Line, Tumor; D019175:DNA Methylation; D006801:Humans; D007621:Karyotyping; D015470:Leukemia, Myeloid, Acute / Q000235:genetics* / Q000401:mortality; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D011401:Promoter Regions, Genetic
D022762:CCAAT-Enhancer-Binding Proteins; C515848:CEBPA protein, human; D035683:MicroRNAs; C487931:mirnlet7 microRNA, human
Acute myeloid leukemia; CCAAT/enhancer binding protein α; DNA methylation; miRNA let-7a-3