A Global Expression Switch Marks Pachytene Initiation during Mouse Male Meiosis.
Male spermatogenesis is an essential and complex process necessary to gain totipotency and allow a whole new organism to develop upon fertilization. While single-gene based studies have provided insights into the mechanisms underlying spermatogenesis, detailed global profiling of all the key meiotic stages is required to fully define these processes. Here, by isolating highly enriched mouse meiotic cell populations, we have generated a comprehensive gene expression atlas of mammalian meiosis. Our data define unique signatures for the specific stages of meiosis, including global chromosome X inactivation and reactivation. The data also reveal profound switches in global gene expression at the initiation of pachynema that are reminiscent of the commitment to meiosis observed in budding yeast. Overall, this meiotic atlas provides an exhaustive blueprint and resource for mammalian gametogenesis and meiosis.
Genome Plasticity Laboratory, Department of Cancer Biology, The Scripps Research Institute, Scripps Florida, Jupiter, FL 33458, USA. mfallahi@scripps.edu.
Mohammad
M
Fallahi
Genome Plasticity Laboratory, Department of Cancer Biology, The Scripps Research Institute, Scripps Florida, Jupiter, FL 33458, USA. igetun@scripps.edu.
Irina V
IV
Getun
Genome Plasticity Laboratory, Department of Cancer Biology, The Scripps Research Institute, Scripps Florida, Jupiter, FL 33458, USA. zhenkwu@scripps.edu.
Zhen K
ZK
Wu
Genome Plasticity Laboratory, Department of Cancer Biology, The Scripps Research Institute, Scripps Florida, Jupiter, FL 33458, USA. pbois@scripps.edu.
Philippe R J
PR
Bois