Inhibitory efficacy of cyclo(L-leucyl-L-prolyl) from mangrove rhizosphere bacterium-Bacillus amyloliquefaciens (MMS-50) toward cariogenic properties of Streptococcus mutans.
Since Streptococcus mutans is the principal etiologic agent causing dental caries, by encompassing an array of unique virulence traits, emerging treatment strategies that specifically target the virulence of this pathogen may be promising as alternative approaches compared to conventional antibiotic therapy. In this perspective, we investigated chloroform extract of cell-free culture supernatant from mangrove rhizosphere bacterium Bacillus amyloliquefaciens (MMS-50) in terms of anticariogenic properties of S. mutans, without suppressing its viability. Crude chloroform extract of MMS-50 was subjected to column and high performance liquid chromatographic techniques to obtain the active fraction (AF), and MMS-50 AF was used for all further assays. GC-MS and FT-IR were carried out to identify the major components present in MMS-50 AF. Comparative gene expression analysis of some biofilm-forming and virulence genes (vicR, comDE, gtfC, and gbpB) was done by real-time PCR. Cyclo(L-leucyl-L-prolyl) was found to be the chief compound in MMS-50 AF responsible for bioactivity. The minimum and maximum inhibitory concentrations of MMS-50 AF against S. mutans were found to be 100 and 250 μg/mL, respectively. Anti-virulence assays performed using below-sub-MIC levels of MMS-50 AF (30 μg/mL) resulted in significant reduction in adherence (68%), acid production, acid tolerance, glucan synthesis (32%), biofilm formation (53.5%) and cell surface hydrophobicity, all devoid of affecting its viability. The micrographs of CLSM and SEM further confirmed the antibiofilm and anti-virulence efficacies of MMS-50 AF. Expression data showed significant reduction in expression of all studied virulence genes. Thus, the current study unveils the anticariogenic potential of cyclo(L-leucyl-L-prolyl) from B. amyloliquefaciens, as well as its suitability as a novel and alternative anticariogenic agent against dental caries.
Department of Biotechnology, Alagappa University, Science Campus, Karaikudi 630004, Tamil Nadu, India.
Shanmugaraj
S
Gowrishankar
Department of Biotechnology, Alagappa University, Science Campus, Karaikudi 630004, Tamil Nadu, India.
Balan
B
Poornima
Department of Biotechnology, Alagappa University, Science Campus, Karaikudi 630004, Tamil Nadu, India. Electronic address: sk_pandian@rediffmail.com.
Shunmugiah Karutha
SK
Pandian
D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D001407:Bacillus / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D001422:Bacterial Adhesion / Q000187:drug effects; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D018441:Biofilms / Q000187:drug effects; D003731:Dental Caries / Q000382:microbiology*; D004151:Dipeptides / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D010456:Peptides, Cyclic / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D058441:Rhizosphere; D012988:Soil Microbiology; D013295:Streptococcus mutans / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004151:Dipeptides; D010456:Peptides, Cyclic; C118540:cyclo(phenylalanyl-prolyl)
Anticariogenic agent; Bacillus amyloliquefaciens; Glucosyltransferases; Mangrove rhizosphere bacteria