miR-10a and miR-10b target the 3'-untranslated region of TBX5 to repress its expression.
As a well-known transcription factor, TBX5 is involved in embryonic cardiac development. Although TBX5 functions in a dose-dependent manner, the posttranscriptional regulation of human TBX5 is poorly understood. Thus, this study aimed to identify microRNAs that modulate TBX5 expression. Luciferase assays were used to screen miRNAs predicted to modulate TBX5 expression. Using quantitative reverse transcriptase-polymerase chain reaction and Western blot analysis, the authors found that miR-10a and miR-10b significantly repressed TBX5 expression and decreased TBX5 protein levels by targeting the TBX5 3'-untranslated region. In addition, miR-10a and miR-10b expression levels were respectively 2.77 and 3.51 times higher in the heart tissues of congenital heart disease patients than in healthy control subjects, suggesting that they are potential diagnostic biomarkers. In conclusion, the study results indicate that miR-10a and miR-10b inhibit TBX5 expression at the level of translation. Higher levels of miR-10a and miR-10b expression are associated with a higher risk of congenital heart defects.
Department of Cardiology, Children's Hospital of Fudan University, 399 Wanyuan Road, Shanghai, 201102, China.
Feng
F
Wang
Xue-Yan
XY
Yang
Jian-Yuan
JY
Zhao
Li-Wei
LW
Yu
Ping
P
Zhang
Wen-Yuan
WY
Duan
Mei
M
Chong
Yong-Hao
YH
Gui
D020413:3' Untranslated Regions; D002681:China; D005260:Female; D005786:Gene Expression Regulation; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease; D006330:Heart Defects, Congenital / Q000235:genetics*; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D011499:Protein Processing, Post-Translational; D020825:T-Box Domain Proteins / Q000235:genetics / Q000378:metabolism
D020413:3' Untranslated Regions; D005819:Genetic Markers; C547183:MIRN10 microRNA, human; D035683:MicroRNAs; D020825:T-Box Domain Proteins; C103172:T-box transcription factor 5