Comparison of oxidative stress in four Tillandsia species exposed to cadmium.
This is first study comparing four morphologically variable species of the genus Tillandsia and therefore various responses to the cadmium (Cd) action were expected. In accordance, Cd accumulation increased in order Tillandsia fasciculata < Tillandsia brachycaulos < Tillandsia pruinosa < Tillandsia capillaris, reaching 29.6 and 197.4 μg g(-1) DW in first and last species after watering with 2 μM Cd(2+) solution over 30 days. Fluorescence visualization of oxidative stress confirmed increase in ROS and especially elevation in hydroperoxides though no visible symptoms appeared on the plants. At the same time, nitric oxide generation and nitroso-glutathione depletion by Cd treatment were typically observed. Fluorescence staining of Cd using two dyes (PhenGreen and Leadmium) showed that Leadmium fits better with AAS quantification. Macro- and micro-nutrients were not considerably affected except for zinc. Reduced glutathione content was the highest in control T. fasciculata while oxidized glutathione in T. capillaris. Ascorbic acid amount revealed extreme quantitative differences among species and decreased in T. fasciculata only. Free amino acids accumulation was similar among species except for T. capillaris and Cd caused both depletion and increase but without high quantitative differences. Data are explanatively discussed in the context of limited literature related to oxidative stress in epiphytic plants and with general responses of plants to cadmium/heavy metals.
Institute of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemědělská 1, 613 00 Brno, Czech Republic. Electronic address: jozkovacik@yahoo.com.
Jozef
J
Kováčik
Department of Natural Drugs, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, Palackého 1/3, 612 42 Brno, Czech Republic.
Petr
P
Babula
Institute of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemědělská 1, 613 00 Brno, Czech Republic.
Bořivoj
B
Klejdus
Institute of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemědělská 1, 613 00 Brno, Czech Republic.
Josef
J
Hedbavny
D002104:Cadmium / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism; D008856:Microscopy, Fluorescence; D018384:Oxidative Stress / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000378:metabolism; D031232:Tillandsia / Q000187:drug effects* / Q000378:metabolism*
D017382:Reactive Oxygen Species; D002104:Cadmium; D005978:Glutathione
Air pollution; Antioxidants; Fluorescence microscopy; GSNO; Reactive oxygen species