Whole ovine ovaries as a model for human: perfusion with cryoprotectants in vivo and in vitro.
These experiments were performed to test the perfusion of ovine as a model for human ovaries by cryoprotectants in vivo at high temperature when the permeability of capillaries is high and when blood is insensibly replaced by the solution of cryoprotectants. By our hypothetical supposition, ovaries could be saturated by cryoprotectants before their surgical removal. The objective was to examine the effectiveness of perfusion of ovine ovaries with vascular pedicle in vivo and in vitro. Arteria ovarica was cannuled and ovaries were perfused by Leibovitz L-15 medium + 100 IU/mL heparin + 5% bovine calf serum + 6% dimethyl sulfoxide + 6% ethylene glycol + 0.15 M sucrose + Indian ink in vivo and in vitro. In the first and second cycle of experiments, ovaries (n = 13 and n = 23) were perfused in vivo and in vitro, respectively, during 60 min with the rate of perfusion 50 mL/h (0.8 mL/min). It was established with in vivo perfusion that only about 10% of ovarian tissues were perfused due to an appearance of multiple anastomoses when the perfusion medium goes from arteria ovarica to arteria uterina without inflow into the ovaries. It was concluded that in vitro perfusion of ovine intact ovaries with vascular pedicle by freezing medium is more effective than this manipulation performed in vivo.
Department of Obstetrics and Gynecology, Cologne University, Kerpener Stra β e 34, 50931 Cologne, Germany.
Vladimir
V
Isachenko
Department of Obstetrics and Gynecology, Cologne University, Kerpener Stra β e 34, 50931 Cologne, Germany.
Gohar
G
Rahimi
Agris-Sardegna, DIRPA, Reproduction Division, S.S. 291 Km, 7100 Sassari, Italy.
Maria
M
Dattena
Department of Obstetrics and Gynecology, Cologne University, Kerpener Stra β e 34, 50931 Cologne, Germany.
Peter
P
Mallmann
Faculty of Biology and Biotechnology, Al-Farabi Kazakh National University, Prospekt Al-Farabi 71, Almaty 50040, Kazakhstan.
Saltanat
S
Baikoshkarova
Teraerzte-Team K-Suelz, Rennebergstra β e 23, 50939 Cologne, Germany.
Elisabeth
E
Kellerwessel
Faculty of Biology and Biotechnology, Al-Farabi Kazakh National University, Prospekt Al-Farabi 71, Almaty 50040, Kazakhstan.
Marat
M
Otarbaev
Faculty of Biology and Biotechnology, Al-Farabi Kazakh National University, Prospekt Al-Farabi 71, Almaty 50040, Kazakhstan.
Tamara
T
Shalakhmetova
Department of Obstetrics and Gynecology, Cologne University, Kerpener Stra β e 34, 50931 Cologne, Germany.
Evgenia
E
Isachenko
D000818:Animals; D002417:Cattle; D015925:Cryopreservation; D003451:Cryoprotective Agents / Q000008:administration & dosage*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D010053:Ovary / Q000166:cytology* / Q000254:growth & development; D010477:Perfusion; D012756:Sheep
D003451:Cryoprotective Agents