Seco-pregnane steroidal glycosides from the roots of Cynanchum atratum and their anti-TMV activity.
Fifteen new seco-pregnane steroidal glycosides cynanosides A-O (1-15) together with twenty-seven known ones were isolated from the roots of Cynanchum atratum. The structures of 1-15 were determined by extensive analysis of spectroscopic data. The anti-tobacco mosaic virus (TMV) activity of these steroidal glycosides was screened by the conventional half-leaf method, enzyme-linked immunosorbent assay, and Western blot methods, most of them showed potent anti-TMV activity. Among them, compounds 1, 7, 13, 28 and 31 showed significantly anti-TMV activity with an IC50 value of 20.5, 18.6, 22.0, 19.2 and 22.2 μg/mL, respectively, and were much more effective than the positive control, ningnanmycin (IC50=49.6 μg/mL).
Center for Research and Development of Fine Chemicals, Guizhou University, Guiyang 550025, People's Republic of China; The Key Laboratory of Chemistry for Natural Product of Guizhou Province and Chinese Academy of Sciences, Guiyang 550002, People's Republic of China.
Ying
Y
Yan
The Key Laboratory of Chemistry for Natural Product of Guizhou Province and Chinese Academy of Sciences, Guiyang 550002, People's Republic of China.
Jian-xin
JX
Zhang
The Key Laboratory of Chemistry for Natural Product of Guizhou Province and Chinese Academy of Sciences, Guiyang 550002, People's Republic of China.
Kai-xing
KX
Liu
The Key Laboratory of Chemistry for Natural Product of Guizhou Province and Chinese Academy of Sciences, Guiyang 550002, People's Republic of China.
Tao
T
Huang
The Key Laboratory of Chemistry for Natural Product of Guizhou Province and Chinese Academy of Sciences, Guiyang 550002, People's Republic of China.
Chen
C
Yan
The Key Laboratory of Chemistry for Natural Product of Guizhou Province and Chinese Academy of Sciences, Guiyang 550002, People's Republic of China.
Lie-jun
LJ
Huang
The Key Laboratory of Chemistry for Natural Product of Guizhou Province and Chinese Academy of Sciences, Guiyang 550002, People's Republic of China.
Sheng
S
Liu
The Key Laboratory of Chemistry for Natural Product of Guizhou Province and Chinese Academy of Sciences, Guiyang 550002, People's Republic of China.
Shu-zhen
SZ
Mu
The Key Laboratory of Chemistry for Natural Product of Guizhou Province and Chinese Academy of Sciences, Guiyang 550002, People's Republic of China. Electronic address: haoxj@mail.kib.ac.cn.
Xiao-jiang
XJ
Hao
D000998:Antiviral Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D010840:Phytosterols / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D010935:Plant Diseases; D018517:Plant Roots / Q000737:chemistry; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D012632:Secosteroids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D014026:Nicotiana / Q000821:virology; D014027:Tobacco Mosaic Virus / Q000187:drug effects*; D066047:Vincetoxicum / Q000737:chemistry*
D000998:Antiviral Agents; D006027:Glycosides; D010840:Phytosterols; D012503:Saponins; D012632:Secosteroids
Anti-tobacco mosaic virus activity; Coat protein; Cynanchum atratum Bunge; Seco-pregnane steroidal glycosides