Lidamycin regulates p53 expression by repressing Oct4 transcription.
Antitumor antibiotic lidamycin (LDM) is widely used in the treatment of a variety of cancers. Here we demonstrated that LDM up-regulates the expression of the tumor suppressor p53 gene by repressing Oct4 transcription. We showed that low dose LDM-induced increase of p53 expression and decrease of Oct4 expression in P19 and HCT116-p53(+/+) cells. Knockdown of Oct4 expression by siRNA led to activation of p53 in both cell lines, whereas ectopical expression of Oct4 significantly inhibited p53 expression in P19 cells. LDM-induced p53 activation was blocked by ectopical expression of Oct4.
Dept of Cell Biology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, China. Electronic address: hyzhen@bjmu.edu.cn.
Hong-Ying
HY
Zhen
Dept of Oncology, The First Hospital of Jinlin University, Changchun, China.
Juan
J
Zhou
Medical Analysis Center, Peking University Health Science Center, Beijing, China.
Hou-Nan
HN
Wu
Dept of Oncology, The First Hospital of Jinlin University, Changchun, China.
Cheng
C
Yao
Dept of Cell Biology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, China.
Ting
T
Zhang
Dept of Cell Biology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, China.
Tong
T
Wu
The Key Laboratory of Pathobiology, Ministry of Education, Bethune Medical College, Jilin University, Changchun, Jilin 310021, China. Electronic address: quancs@jlu.edu.cn.
Cheng-Shi
CS
Quan
The Key Laboratory of Pathobiology, Ministry of Education, Bethune Medical College, Jilin University, Changchun, Jilin 310021, China. Electronic address: ylli@jlu.edu.cn.
Yu-Lin
YL
Li
D000617:Aminoglycosides / Q000494:pharmacology*; D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D045744:Cell Line, Tumor; D053281:Enediynes / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects*; D055785:Gene Knockdown Techniques; D006801:Humans; D051379:Mice; D050814:Octamer Transcription Factor-3 / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000187:drug effects; D034741:RNA, Small Interfering / Q000235:genetics; D014158:Transcription, Genetic / Q000187:drug effects*; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*; D015854:Up-Regulation
D000617:Aminoglycosides; D000903:Antibiotics, Antineoplastic; D053281:Enediynes; D050814:Octamer Transcription Factor-3; D034741:RNA, Small Interfering; D016159:Tumor Suppressor Protein p53; C058024:C 1027
Lidamycin; Oct4; Transcriptional activation; p53