Efficacy against nematode and cestode infections and safety of a novel topical fipronil, (S)-methoprene, eprinomectin and praziquantel combination product in domestic cats under field conditions in Europe.
A novel topical combination product (BROADLINE(®), Merial) composed of fipronil, (S)-methoprene, eprinomectin and praziquantel was evaluated for safety and efficacy against nematode and cestode infections in domestic cats. The study comprised a multi-centre, positive control, blinded, field study, using a randomized block design based on order of presentation for allocation. In total 196 client-owned cats, confirmed as positive for naturally acquired infections of nematodes and/or cestodes by pre-treatment faecal examination, were studied in seven countries in Europe. Pre-treatment faecal examination revealed the presence of Toxocara, hookworm, Capillaria and/or spirurid nematode infections in 129, 73, 33 or 1 cat(s), respectively; infections with taeniid and Dipylidium cestodes were demonstrated in 39 and 17 cats, respectively. Cats were allocated randomly to one of two treatments in a ratio of 2, topical fipronil (8.3%, w/v), (S)-methoprene (10%, w/v), eprinomectin (0.4%, w/v) and praziquantel (8.3%, w/v) (BROADLINE(®), Merial; 130 cats); and 1, topical PROFENDER(®) Spot-On (Bayer; 66 cats) and treated once on Day 0. For evaluation of efficacy, two faecal samples were collected, one prior to treatment (Day -4 ± 4 days) and one at the end of the study (Day 14 ± 5 days). These were examined for fecal forms of nematode and cestode parasites. For evaluation of safety, cats were examined by a veterinarian before treatment and at the end of the study, and cat owners recorded the health status of their cats daily until the end of the study. For cats treated with Broadline(®), the efficacy was >99.9%, 100%, and 99.6% for Toxocara, hookworms, and Capillaria, respectively; and the efficacy was >99.9%, >99.9%, and 98.5%, respectively, for the cats treated with Profender(®) (p<0.001 for all nematodes and both treatments). Efficacy was 100% for both cestodes for both treatments (p<0.001). No treatment related adverse experiences were observed throughout the study. For both treatments, every cat that completed the study was given a safety score of 'excellent' for both local and systemic evaluations. The topical combination product of fipronil, (S)-methoprene, eprinomectin and praziquantel was shown to have an excellent safety profile and demonstrated high levels of efficacy when administered once as topical solution to cats infected with nematodes and cestodes under field conditions.
Merial GmbH, Kathrinenhof Research Center, 83101 Rohrdorf, Germany. Electronic address: steffen.rehbein@merial.com.
Steffen
S
Rehbein
8330 Sümeg, Hungary.
Balazs
B
Capári
Veterinary University Vienna, Institute of Parasitology, 1210 Vienna, Austria.
Georg
G
Duscher
Latvia University of Agriculture, 3001 Jelgava, Latvia.
Dace
D
Keidane
Trakia University, Department of Parasitology, 6000 Stara Zagora, Bulgaria.
Zvezdelina
Z
Kirkova
Lithuanian University of Health Sciences, Veterinary Academy, 47181 Kaunas, Lithuania.
Saulius
S
Petkevičius
Universiteti Bujqësor, Fakulteti i Mjeksise Veterinare, Kodër Kamëz, Tirana, Albania.
Dhimiter
D
Rapti
83122 Samerberg, Germany.
Annegret
A
Wagner
86825 Bad Wörishofen, Germany.
Thomas
T
Wagner
Merial Limited, Duluth, GA 30096, USA.
S Theodore
ST
Chester
Merial Limited, Duluth, GA 30096, USA.
Joseph
J
Rosentel
Merial SAS, Centre de Recherche de Saint-Vulbas, 01150 Saint-Vulbas, France.
Eric
E
Tielemans
Merial GmbH, Kathrinenhof Research Center, 83101 Rohrdorf, Germany.
Martin
M
Visser
Merial GmbH, Kathrinenhof Research Center, 83101 Rohrdorf, Germany.
Renate
R
Winter
Merial GmbH, Kathrinenhof Research Center, 83101 Rohrdorf, Germany.
Katrin
K
Kley
Merial GmbH, Kathrinenhof Research Center, 83101 Rohrdorf, Germany.
Martin
M
Knaus
D000818:Animals; D002371:Cat Diseases / Q000188:drug therapy*; D002415:Cats; D002589:Cestoda; D002590:Cestode Infections / Q000188:drug therapy / Q000662:veterinary*; D004338:Drug Combinations; D005060:Europe; D005260:Female; D007559:Ivermectin / Q000008:administration & dosage / Q000031:analogs & derivatives*; D008297:Male; D008726:Methoprene / Q000008:administration & dosage*; D009348:Nematoda; D009349:Nematode Infections / Q000188:drug therapy / Q000662:veterinary*; D011223:Praziquantel / Q000008:administration & dosage*; D011720:Pyrazoles / Q000008:administration & dosage*; D016896:Treatment Outcome
D004338:Drug Combinations; D011720:Pyrazoles; D011223:Praziquantel; D007559:Ivermectin; C101434:eprinomectin; D008726:Methoprene; C082360:fipronil
(S)-methoprene; Cestodes; Eprinomectin; Fipronil; Nematodes; Praziquantel