Beneficial effects of methylene blue on the acute phase of corrosive esophageal burn in rats.
BACKGROUND
The aim of the article is to investigate the beneficial effects of methylene blue (MB) on the acute phase of experimental corrosive esophageal burns.


METHODS
A total of 21 male Sprague-Dawley rats were allocated into three groups with an equal number in each group. In Group 1 (control), the esophageal lumen was irrigated with saline. In Groups 2 (untreated) and 3 (MB treatment), the distal esophagus was burned with 30% NaOH solution. In Group 3, MB was applied locally on the distal esophageal lumen (30 mg/kg) 90 minutes after the burn. In all the groups, animals were humanely killed at the end of the 7th day. Distal esophageal specimens were obtained for histopathological and biochemical examinations.


RESULTS
MB increased the tissue superoxide dismutase levels and the total antioxidant status, whereas it decreased the levels of the total oxidant status with significant attenuation of histopathological damage.


CONCLUSIONS
MB might have reduced the tissue damage in corrosive esophagitis because of its antioxidant effects.
Department of Pediatric Surgery, Faculty of Medicine, Bezmialem Vakif University, Istanbul, Turkey.
Huseyin
H
Kilincaslan
Department of Pediatrics, Zeynep Kamil Research and Education Hospital, Istanbul, Turkey.
Hande Ozgun
HO
Karatepe
Department of Biochemistry, Faculty of Medicine, Bezmialem Vakif University, Istanbul, Turkey.
Elif
E
Kilic
Department of Pediatric Surgery, Faculty of Medicine, Bezmialem Vakif University, Istanbul, Turkey.
Ibrahim
I
Aydogdu
Department of Pediatrics, Faculty of Medicine, Bezmialem Vakif University, Istanbul, Turkey.
Ahmet Hakan
AH
Gedik
Department of Pathology, Institute of Oncology, Istanbul University, Istanbul, Turkey.
Vakur
V
Olgac
Department of Pediatrics, Faculty of Medicine, Bezmialem Vakif University, Istanbul, Turkey.
Mehmet
M
Kucukkoc
D000818:Animals; D002057:Burns, Chemical / Q000150:complications / Q000378:metabolism / Q000473:pathology*; D002374:Catalase / Q000378:metabolism; D002424:Caustics; D004195:Disease Models, Animal; D004940:Esophageal Stenosis / Q000139:chemically induced / Q000517:prevention & control*; D004941:Esophagitis / Q000139:chemically induced / Q000150:complications / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008751:Methylene Blue / Q000494:pharmacology*; D016877:Oxidants / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism
D002424:Caustics; D016877:Oxidants; D002374:Catalase; D013482:Superoxide Dismutase; D008751:Methylene Blue