Efficacy of a novel topical combination of fipronil, (S)-methoprene, eprinomectin and praziquantel against adult and immature stages of the cat flea (Ctenocephalides felis) on cats.
The efficacy of a novel topical combination of fipronil 8.3% (w/v), (S)-methoprene 10% (w/v), eprinomectin 0.4% (w/v) and praziquantel 8.3% (w/v) (BROADLINE(®)) was tested against adult and immature stages of Ctenocephalides felis fleas in six studies. For that purpose, fleas from different colonies from North America, Germany and South Africa were used to induce infestations in cats under laboratory conditions. In each study, between 12 and 16 cats were allocated randomly to 2 groups. Cats in Group 1 were not treated and served as controls. Cats in Group 2 were treated once on Day 0 with BROADLINE(®) at the minimum recommended dosage of 0.12 mg/kg body weight. In 4 studies, all animals were infested experimentally with unfed C. felis (100 ± 5) on Days 2 (or 1), 7, 14, 21, 28 and 35. Live fleas were counted 24h post-treatment or infestation. In 2 additional studies, animals were infested at the same frequency with gravid C. felis fleas (100 ± 5) that were fed previously on an untreated host. Forty-eight hours post-infestation, flea eggs were collected, counted and incubated for the evaluation of the reduction of emergence of adults. The combined curative efficacy against adult fleas at 24h after treatment was 94.3% and the combined preventive efficacy values remained greater than 95.9% at 24h after 5 subsequent weekly infestations. In addition, the product reduced dramatically the emergence of new adult fleas for at least 5 weeks (>98.1% for one month and 93.2% at 5 weeks after infestation), demonstrating its efficiency in preventing environmental contamination by immature stages.
Merial Limited, 115 Transtech Drive, Athens, GA, USA. Electronic address: christine.baker@merial.com.
Christine
C
Baker
Merial S.A.S., CRSV, 1 allée des Cyprès, 01150 St Vulbas, France.
Eric
E
Tielemans
Merial Limited, 3239 Satellite Boulevard, Duluth, GA 30096, USA.
Joseph B
JB
Prullage
Merial Limited, 3239 Satellite Boulevard, Duluth, GA 30096, USA.
S Theodore
ST
Chester
Merial GmbH, Kathrinenhof Research Center, Walchenseestr. 8-12, 83101 Rohrdorf, Germany.
Martin
M
Knaus
Merial GmbH, Kathrinenhof Research Center, Walchenseestr. 8-12, 83101 Rohrdorf, Germany.
Steffen
S
Rehbein
ClinVet International (Pty) Ltd., Uitsig Road, Bainsvlei, 9321 Bloemfontein, South Africa.
Josephus J
JJ
Fourie
Young Veterinary Research, 7243 East Avenue, Turlock, CA 95380-9124, USA.
David R
DR
Young
BerTek, Inc., 104 Wilson Bottoms Road, Greenbrier, AR 72058, USA.
William R
WR
Everett
Merial Limited, 3239 Satellite Boulevard, Duluth, GA 30096, USA.
Joseph K
JK
Rosentel
D000818:Animals; D000977:Antiparasitic Agents / Q000008:administration & dosage* / Q000494:pharmacology; D002371:Cat Diseases / Q000188:drug therapy* / Q000517:prevention & control; D002415:Cats; D058251:Ctenocephalides / Q000187:drug effects; D004338:Drug Combinations; D058267:Flea Infestations / Q000188:drug therapy / Q000517:prevention & control / Q000662:veterinary*; D007559:Ivermectin / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000494:pharmacology; D008018:Life Cycle Stages / Q000187:drug effects; D008726:Methoprene / Q000008:administration & dosage / Q000494:pharmacology; D011223:Praziquantel / Q000008:administration & dosage / Q000494:pharmacology; D011720:Pyrazoles / Q000008:administration & dosage / Q000494:pharmacology; D011897:Random Allocation; D016896:Treatment Outcome
D000977:Antiparasitic Agents; D004338:Drug Combinations; D011720:Pyrazoles; D011223:Praziquantel; D007559:Ivermectin; C101434:eprinomectin; D008726:Methoprene; C082360:fipronil
(S)-methoprene; Ctenocephalides felis; Efficacy; Eggs; Eprinomectin; Fipronil; Fleas; Topical