In silico Analysis of TCR Vβ7 of Two Patients with Type 1 Diabetes Mellitus.
OBJECTIVE
To compare the sequences and crystal structures of variable region of beta chain 7 (Vβ7) of T cell receptor (TCR) of two patients with type 1 diabetes mellitus (T1DM).


METHODS
The skewness of TCR Vβ7 of two T1DM patients were detected with real-time florescence quantitative polymerase chain reaction (FQ-PCR) and deoxyribonucleic acid (DNA) melting curve analysis technique followed by being sequenced, the crystal structures of them were simulated according to CPH models 2.0 Server, IMGT database, and RasMol 2 software.


RESULTS
The whole sequences of TCR Vβ7 of T1DM patient-1 were "CASRTAGQYEQYFGPGTR", that of patient-2 were "CASRTAGQYEQFFGPGTR"; the only difference between them lied on the 12(th) amino acid. The crystal structures of Vβ7 of the two patients simulated with backbone model were rather similar, while that with sphere model were obviously different.


CONCLUSIONS
Although the TCR Vβ7 of the T1DM patients share the similar gene sequences, their crystal structures simulated with sphere model are different, and the mechanism needs further study.
Clinic Laboratory, The Affiliated Hospital of Jining Medical College, Jining, Shandong Province, China.
Jianwei
J
Zhou
Department of Cardiovascular Disease, The Affiliated Hospital of Jining Medical College, Jining, Shandong Province, China.
Cui
C
Kong
Department of Stomatology, The Affiliated Hospital of Jining Medical College, Jining, Shandong Province, China.
Xiukui
X
Wang
Clinic Laboratory, The Affiliated Hospital of Jining Medical College, Jining, Shandong Province, China.
Yinfeng
Y
Jia
Department of Pharmocology, The First People's Hospital, Jining, Shandong Province, China.
Li
L
Wang
Clinic Laboratory, The Affiliated Hospital of Jining Medical College, Jining, Shandong Province, China.
Hong
H
Chang
Department of Endocrinology, The Affiliated Hospital of Jining Medical College, Jining, Shandong Province, China.
Lin
L
Sun
Beta chain; T cell receptor; crystal structure; type 1 diabetes mellitus; variable region