Molybdenum trioxide nanoparticles with intrinsic sulfite oxidase activity.
Sulfite oxidase is a mitochondria-located molybdenum-containing enzyme catalyzing the oxidation of sulfite to sulfate in the amino acid and lipid metabolism. Therefore, it plays a major role in detoxification processes, where defects in the enzyme cause a severe infant disease leading to early death with no efficient or cost-effective therapy in sight. Here we report that molybdenum trioxide (MoO3) nanoparticles display an intrinsic biomimetic sulfite oxidase activity under physiological conditions, and, functionalized with a customized bifunctional ligand containing dopamine as anchor group and triphenylphosphonium ion as targeting agent, they selectively target the mitochondria while being highly dispersible in aqueous solutions. Chemically induced sulfite oxidase knockdown cells treated with MoO3 nanoparticles recovered their sulfite oxidase activity in vitro, which makes MoO3 nanoparticles a potential therapeutic for sulfite oxidase deficiency and opens new avenues for cost-effective therapies for gene-induced deficiencies.
Institut für Anorganische Chemie und Analytische Chemie, Johannes-Gutenberg-Universität , Duesbergweg 10-14, D-55099 Mainz, Germany.
Ruben
R
Ragg
Filipe
F
Natalio
Muhammad Nawaz
MN
Tahir
Henning
H
Janssen
Anubha
A
Kashyap
Dennis
D
Strand
Susanne
S
Strand
Wolfgang
W
Tremel
D000592:Amino Acid Metabolism, Inborn Errors; D001665:Binding Sites; D004563:Electrochemistry; D004566:Electrodes; D004581:Electronics; D007700:Kinetics; D008027:Light; D053768:Metal Nanoparticles / Q000737:chemistry*; D008982:Molybdenum / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods*; D053770:Nanowires / Q000737:chemistry; D010084:Oxidation-Reduction; D010087:Oxides / Q000737:chemistry*; D010777:Photochemistry; D017785:Photons; D050876:Sulfite Oxidase / Q000737:chemistry* / Q000172:deficiency; D015034:Zinc Oxide / Q000737:chemistry
D010087:Oxides; C082290:molybdenum trioxide; D008982:Molybdenum; D050876:Sulfite Oxidase; D015034:Zinc Oxide