Synergism between ultrasonic pretreatment and white rot fungal enzymes on biodegradation of wheat chaff.
Lignocellulosic biomass samples (wheat chaff) were pretreated by ultrasound (US) (40kHz/0.5Wcm(-2)/10min and 400kHz/0.5Wcm(-2)/10min applied sequentially) prior to digestion by enzyme extracts obtained from fermentation of the biomass with white rot fungi (Phanerochaete chrysosporium or Trametes sp.). The accessibility of the cellulosic components in wheat chaff was increased, as demonstrated by the increased concentration of sugars produced by exposure to the ultrasound treatment prior to enzyme addition. Pretreatment with ultrasound increased the concentration of lignin degradation products (guaiacol and syringol) obtained from wheat chaff after enzyme addition. In vitro digestibility of wheat chaff was also enhanced by the ultrasonics pretreatment in combination with treatment with enzyme extracts. Degradation was enhanced with the use of a mixture of the enzyme extracts compared to that for a single enzyme extract.
CSIRO Animal Food and Health Sciences, 671 Sneydes Road, Werribee, VIC 3030, Australia. Electronic address: henry.sabarez@csiro.au.
Henry
H
Sabarez
CSIRO Animal Food and Health Sciences, 671 Sneydes Road, Werribee, VIC 3030, Australia.
Christine Maree
CM
Oliver
CSIRO Animal Food and Health Sciences, 671 Sneydes Road, Werribee, VIC 3030, Australia.
Raymond
R
Mawson
CSIRO Material Science and Engineering, Gate 5, Normanby Road, Clayton, VIC 3168, Australia.
Geoff
G
Dumsday
CSIRO Animal Food and Health Sciences, 671 Sneydes Road, Werribee, VIC 3030, Australia.
Tanoj
T
Singh
CSIRO Material Science and Engineering, Gate 5, Normanby Road, Clayton, VIC 3168, Australia.
Natalie
N
Bitto
CSIRO Animal Food and Health Sciences, 671 Sneydes Road, Werribee, VIC 3030, Australia.
Chris
C
McSweeney
CSIRO Animal Food and Health Sciences, 671 Sneydes Road, Werribee, VIC 3030, Australia.
Mary Ann
MA
Augustin
D018533:Biomass; D005285:Fermentation; D042845:Laccase / Q000378:metabolism; D008031:Lignin / Q000737:chemistry / Q000378:metabolism; D020075:Phanerochaete / Q000201:enzymology*; D010636:Phenols / Q000737:chemistry / Q000378:metabolism; D013696:Temperature; D055454:Trametes / Q000201:enzymology*; D014908:Triticum / Q000737:chemistry*; D014465:Ultrasonics
D010636:Phenols; C036909:lignocellulose; D008031:Lignin; D042845:Laccase
Lignolytic enzymes; Phenolics; Rumen digestibility; Sugars; Ultrasound; Wheat chaff