The follicular outcome after standard gonadotropin stimulation is associated with ERα and ERβ genotypes.
The aim is to study the association of estrogen receptor α (ERα) and estrogen receptor β (ERβ) gene polymorphisms and diplotypes with ovarian response to follicle-stimulating hormone (FSH) stimulation and the hormone levels [FSH, luteinizing hormone (LH), E2] at the third day of the menstrual cycle. Three hundred women undergoing medically assisted reproduction and 300 women with at least one spontaneous pregnancy participated in the study. ERα PvuII and XbaI polymorphisms as well as ERβ AluI polymorphism were genotyped. The FSH, LH, and E2 levels were determined at the third day of the menstrual cycle, while the follicular size, the follicle, and oocyte numbers were recorded during oocyte retrieval. PvuII CC, XbaI GG, and AluI GG women were characterized by increased amounts of large follicles compared to PvuII TT, XbaI AA, and AluI AA women (p = 0.045, 0.01, and 0.033, respectively). The PvuII CC/XbaI GG diplotype was also significantly associated with higher large follicle numbers compared to the PvuII TT/XbaI AA diplotype (p = 0.024). However, no associations were observed between ER gene polymorphisms and the hormonal profile, the follicle/oocyte numbers, and the pregnancy rates. ERα and ERβ genetic variants were associated with ovarian response to standard gonadotropin stimulation of women undergoing in-vitro fertilization affecting mainly the follicular growth.
Genetics and IVF Unit, Department of Obstetrics and Gynecology, Medical School, Ioannina University, 45110, Ioannina, Greece.
Leandros
L
Lazaros
Christina
C
Pamporaki
Nikolaos
N
Vlahos
Atsushi
A
Takenaka
Chrysoula
C
Kitsou
Ioannis
I
Kosmas
Nikolaos
N
Sofikitis
Theodoros
T
Stefos
Konstantinos
K
Zikopoulos
Elissavet
E
Hatzi
Ioannis
I
Georgiou
D000328:Adult; D000483:Alleles; D004958:Estradiol / Q000097:blood; D047628:Estrogen Receptor alpha / Q000235:genetics*; D047629:Estrogen Receptor beta / Q000235:genetics*; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood / Q000494:pharmacology / Q000627:therapeutic use; D056726:Genetic Association Studies; D005838:Genotype; D006801:Humans; D016729:Leuprolide / Q000494:pharmacology / Q000627:therapeutic use*; D007986:Luteinizing Hormone / Q000097:blood; D006080:Ovarian Follicle / Q000166:cytology / Q000187:drug effects*; D010062:Ovulation Induction; D020641:Polymorphism, Single Nucleotide; D011247:Pregnancy; D016896:Treatment Outcome
C506487:ESR1 protein, human; D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D004958:Estradiol; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D016729:Leuprolide