Spectrum of Fates: a new approach to the study of the developing zebrafish retina.
The ability to image cells live and in situ as they proliferate and differentiate has proved to be an invaluable asset to biologists investigating developmental processes. Here, we describe a Spectrum of Fates approach that allows the identification of all the major neuronal subtypes in the zebrafish retina simultaneously. Spectrum of Fates is based on the combinatorial expression of differently coloured fluorescent proteins driven by the promoters of transcription factors that are expressed in overlapping subsets of retinal neurons. Here, we show how a Spectrum of Fates approach can be used to assess various aspects of neural development, such as developmental waves of differentiation, neuropil development, lineage tracing and hierarchies of fates in the developing zebrafish retina.
Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge CB2 3DY , UK.
Alexandra D
AD
Almeida
Henrik
H
Boije
Renee W
RW
Chow
Jie
J
He
Jonathan
J
Tham
Sachihiro C
SC
Suzuki
William A
WA
Harris
D000818:Animals; D030801:Animals, Genetically Modified; D002454:Cell Differentiation; D019070:Cell Lineage; D049109:Cell Proliferation; D002478:Cells, Cultured; D002999:Clone Cells; D005453:Fluorescence; D005821:Genetic Techniques; D008164:Luminescent Proteins / Q000378:metabolism*; D012160:Retina / Q000166:cytology / Q000196:embryology*; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics*
D008164:Luminescent Proteins
Cell fate; Differentiation; Fluorescent proteins; Neuron; Retina; Zebrafish