Detection of enrofloxacin and its metabolite ciprofloxacin using gold nanoparticles and laser desorption/ionization time-of-flight mass spectrometry.
This paper describes a new, simple, and sensitive method for detecting two fluoroquinolones: enrofloxacin and its metabolite ciprofloxacin, which are widely used as drugs for humans and animals. We utilized gold nanoparticles (AuNPs) and laser desorption/ionization time-of-flight (LDI-TOF) mass spectrometry (MS) with a matrix-free format. An antibody for the drug was immobilized on a chip based on self-assembled monolayers (SAMs) on gold, and AuNPs were decorated with the drug along with a large excess of small molecules, called amplification tags (Am-tags). In this strategy, target drugs in solution bound to the antibody on the chip compete with the AuNP-immobilized drugs. The presence of targets was verified by the amplified LDI-TOF MS signals of Am-tags on AuNPs.
Department of Bioscience and Biotechnology, Konkuk University.
Mi-Sun
MS
Ha
Hyunjung
H
Seo
Dong-Ho
DH
Bae
Woon-Seok
WS
Yeo
D002138:Calibration; D002939:Ciprofloxacin / Q000032:analysis* / Q000378:metabolism; D000077422:Enrofloxacin; D024841:Fluoroquinolones / Q000032:analysis* / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D024841:Fluoroquinolones; D000077422:Enrofloxacin; D002939:Ciprofloxacin; D006046:Gold