A novel microfluidic system for the rapid analysis of protein thermal stability.
We describe a simple microfluidic device for the rapid analysis of protein thermal stability using a novel imaging method. The change in UV absorption upon thermal denaturation or aggregation of proteins is used to get a spatial image of proteins' folding or aggregation state along a linear temperature gradient.
Jiangsu-Singapore Joint Research Center for Organic/Bio-Electronics & Information Displays and Institute of Advanced Materials (IAM), Nanjing Tech University, 30 South Puzhu Road, Nanjing 211816, P. R. China. iamxyang@njtech.edu.cn.
Xin
X
Yang
Jia
J
Liu
Ye Lei
YL
Xie
Yang
Y
Wang
Hong
H
Ying
Qiong
Q
Wu
Wei
W
Huang
Gareth
G
Jenkins
D044085:Microfluidics / Q000295:instrumentation*; D011506:Proteins / Q000737:chemistry*; D013696:Temperature
D011506:Proteins