Proteomic analysis of pancreas in miniature pigs according to developmental stages using two-dimensional electrophoresis and matrix-assisted laser desorption/ionization-time of flight mass spectrometry.
Organ transplantation is limited by the shortage of human organs. Many studies have sought to overcome this hurdle by using animal organs. Porcine organs, especially from miniature pigs, have been used for organ xenotransplantation rather than nonhuman primates. While the molecular profiling for transplantation is well known in humans and rodents, the situation for pigs is almost completely unknown. The present study examined protein regulation of the developing stages of the pancreatic proteome (4 day-old miniature neonate, 19 day-old miniature piglet, and 14 month-old miniature adult pigs) using two-dimensional gel electrophoresis and matrix assisted laser desorption/ionization-time of flight mass spectrometry. Thirteen different expressed spots were observed and nine were identified. The data presented within this study provides critical direction relating to the development of pancreas of miniature pigs, which will assist future proteome analysis of the pancreas, and advance our understanding of the hurdles facing xenotransplantation.
Department of Biomedical Laboratory Science, College of Biomedical Sciences, Soonchunhyang University, Asan, Korea.
Sun Shin
SS
Yi
Laboratory of Developmental Biology and Genetics, College of Veterinary Medicine, BK21 Plus Program for Creative Science Research, BIO-MAX institute, Seoul National University, Seoul, Korea.
Il Yong
IY
Kim
Department of Pathology and Laboratory of Immune Regulation, College of Medicine, Seoul National University, Seoul, Korea.
Sae Jin
SJ
Oh
Transitional Research Institute, College of Medicine, Seoul National University, Seoul, Korea.
Hye Jung
HJ
Yeom
Biomedical Center for Animal Resource and Development, BIO-MAX Institute, Seoul National University, Seoul, Korea.
Su Cheong
SC
Yeom
Department of Biochemistry, Hanyang University, Ansan, Korea.
Seung Yong
SY
Hwang
Laboratory of Developmental Biology and Genetics, College of Veterinary Medicine, BK21 Plus Program for Creative Science Research, BIO-MAX institute, Seoul National University, Seoul, Korea.
Je Kyung
JK
Seong
2-DE MALDI-TOF; Human organ; miniature pig; pancreas; proteome