Orthogonal array deciphering MRS medium requirements for isolated Lactobacillus rhamnosus ZY with cell properties characterization.
Lactobacillus rhamnosus is a well-known lactic acid bacterium (LAB), but a new ZY strain was isolated for the first time from commercial probiotic powder recently. Although many studies have focused on developing cost-effective media for the production of LAB, the de Man, Rogosa and Sharpe (MRS) medium is still the most common medium for bioprocesses. The aim of the current study is to decipher the composition of MRS based on a statistical approach, which will allow a higher biomass of Lactobacillus to be obtained. In Taguchi's approach, an L27 orthogonal array was adopted to evaluate the significance of 10 ingredients in MRS, in which the effects of the components were ranked according to their effect on biomass at OD600 as dextrose > MnSO4·H2O > beef extract > CH3COONa > MgSO4 > yeast extract > proteose peptone > K2HPO4 > ammonium citrate > Tween 80. Although the individual trace elements of ammonium citrate, K2HPO4, CH3COONa and MgSO4 in MRS had an insignificant influence on the biomass after statistical analysis, the total elimination of trace elements would predominantly affect the cell growth of Lactobacillus. Further characterization of the cell properties through attenuated total reflectance of Fourier transform infrared (ATR-FTIR) spectroscopy and protein identification via SDS-PAGE coupled with tandem mass spectrometry implied that dextrose as major carbon source in MRS played the most crucial role for L. rhamnosus production.
Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
Yu
Y
Zhang
Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China; The Key Laboratory for Synthetic Biotechnology of Xiamen City, Xiamen University, Xiamen 361005, China. Electronic address: yswu@xmu.edu.cn.
I-Son
IS
Ng
Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China; The Key Laboratory for Synthetic Biotechnology of Xiamen City, Xiamen University, Xiamen 361005, China.
Chuanyi
C
Yao
Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China; The Key Laboratory for Synthetic Biotechnology of Xiamen City, Xiamen University, Xiamen 361005, China.
Yinghua
Y
Lu
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D018533:Biomass; D002244:Carbon / Q000378:metabolism; D045424:Complex Mixtures / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry*; D005163:Factor Analysis, Statistical; D005947:Glucose / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism; D052201:Lacticaseibacillus rhamnosus / Q000378:metabolism*; D008969:Molecular Sequence Data; D019936:Probiotics / Q000302:isolation & purification / Q000378:metabolism*; D012492:Salts / Q000378:metabolism; D014131:Trace Elements / Q000378:metabolism
D001426:Bacterial Proteins; D045424:Complex Mixtures; D003470:Culture Media; D012492:Salts; D014131:Trace Elements; D019344:Lactic Acid; D002244:Carbon; D005947:Glucose
Cell property; Fourier transform infrared spectroscopy; Lactobacillus rhamnosus; Medium parameters; Orthogonal array