1.15 Å resolution structure of the proteasome-assembly chaperone Nas2 PDZ domain.
The 26S proteasome is a 2.5 MDa protease dedicated to the degradation of ubiquitinated proteins in eukaryotes. The assembly of this complex containing 66 polypeptides is assisted by at least nine proteasome-specific chaperones. One of these, Nas2, binds to the proteasomal AAA-ATPase subunit Rpt5. The PDZ domain of Nas2 binds to the C-terminal tail of Rpt5; however, it does not require the C-terminus of Rpt5 for binding. Here, the 1.15 Å resolution structure of the PDZ domain of Nas2 is reported. This structure will provide a basis for further insights regarding the structure and function of Nas2 in proteasome assembly.
Division of Biology, Kansas State University, 338 Ackert Hall, Manhattan, KS 66506, USA.
Chingakham R
CR
Singh
Protein Structure Laboratory, University of Kansas, Del Shankel Structural Biology Center, Lawrence, KS 66047, USA.
Scott
S
Lovell
Protein Structure Laboratory, University of Kansas, Del Shankel Structural Biology Center, Lawrence, KS 66047, USA.
Nurjahan
N
Mehzabeen
Division of Biology, Kansas State University, 338 Ackert Hall, Manhattan, KS 66506, USA.
Wasimul Q
WQ
Chowdhury
Division of Biology, Kansas State University, 338 Ackert Hall, Manhattan, KS 66506, USA.
Eric S
ES
Geanes
IMCA-CAT Hauptman-Woodward Medical Research Institute, 9700 South Cass Avenue, Building 435A, Argonne, IL 60439, USA.
Kevin P
KP
Battaile
Division of Biology, Kansas State University, 338 Ackert Hall, Manhattan, KS 66506, USA.
Jeroen
J
Roelofs
D000251:Adenosine Triphosphatases / Q000737:chemistry / Q000378:metabolism*; D000595:Amino Acid Sequence; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods*; D008958:Models, Molecular; D018832:Molecular Chaperones / Q000737:chemistry*; D008969:Molecular Sequence Data; D054731:PDZ Domains; D011487:Protein Conformation; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
D018832:Molecular Chaperones; C114475:NAS2 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D000251:Adenosine Triphosphatases; C541345:RPT5 protein, S cerevisiae
Nas2; PDZ domain; chaperones; proteasome