Effects of quercetin on CYP450 and cytokines in Aroclor 1254 injured endometrial cells of the pregnant rats.
Polychlorinated biphenyls (PCBs) are widespread persistent residual environmental pollutants, which affect seriously the growth and reproductive alterations in humans and animals. Aroclor 1254 is a commercial mixture of PCBs. Quercetin is a flavonoid, which acts on estrogen receptors and causes the development of estrogen-related diseases. In this paper, the primary cultured endometrial cells in the pregnant rats were isolated and Aroclor 1254 was used to induce the injured endometrial cells model. The cells were treated with gradient quercetin, the viability of the endometrial cells, the expressions of CYP450, the contents of TNF-α, IL-6, estradiol (E2), and progesterone (P4) were measured. It showed that the viability of the cultured endometrial cells, the expression of CYP1A1 and CYP2B1, and the contents of TNF-α, E2, and IL-6 in the injured endometrial cells increased with the treatment of quercetin. It shows that quercetin has protective effect on the injured endometrial cells in the pregnant rats, this provide a basis on herbal medicine protection for animal reproductive diseases caused by environmental endocrine disruptors.
College of Veterinary Medicine, Agricultural University of Hebei, Baoding, Hebei 071001, China.
Lina
L
Xu
College of Veterinary Medicine, Agricultural University of Hebei, Baoding, Hebei 071001, China.
Liyun
L
Sun
College of Veterinary Medicine, Agricultural University of Hebei, Baoding, Hebei 071001, China.
Liqin
L
Lu
Institute of Traditional Chinese Veterinary Medicine, Agricultural University of Hebei, Baoding, Hebei 071001, China.
Xiuhui
0000-0001-6757-3776
X
Zhong
College of Veterinary Medicine, Agricultural University of Hebei, Baoding, Hebei 071001, China.
Yuzhong
0000-0003-0101-5784
Y
Ma
College of Veterinary Medicine, Agricultural University of Hebei, Baoding, Hebei 071001, China.
Jianhua
J
Qin
D000818:Animals; D020111:Chlorodiphenyl (54% Chlorine) / Q000633:toxicity*; D019363:Cytochrome P-450 CYP1A1 / Q000096:biosynthesis*; D019362:Cytochrome P-450 CYP2B1 / Q000096:biosynthesis*; D004717:Endometrium / Q000166:cytology / Q000187:drug effects; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis; D011247:Pregnancy; D011374:Progesterone / Q000096:biosynthesis; D011794:Quercetin / Q000008:administration & dosage*; D051381:Rats; D011960:Receptors, Estrogen / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D015850:Interleukin-6; D011960:Receptors, Estrogen; D014409:Tumor Necrosis Factor-alpha; D020111:Chlorodiphenyl (54% Chlorine); D011374:Progesterone; D011794:Quercetin; D019363:Cytochrome P-450 CYP1A1; D019362:Cytochrome P-450 CYP2B1