Start-up of an anaerobic dynamic membrane digester for waste activated sludge digestion: temporal variations in microbial communities.
An anaerobic dynamic membrane digester (ADMD) was developed to digest waste sludge, and pyrosequencing was used to analyze the variations of the bacterial and archaeal communities during the start-up. Results showed that bacterial community richness decreased and then increased over time, while bacterial diversity remained almost the same during the start-up. Proteobacteria and Bacteroidetes were the major phyla. At the class level, Betaproteobacteria was the most abundant at the end of start-up, followed by Sphingobacteria. In the archaeal community, richness and diversity peaked at the end of the start-up stage. Principle component and cluster analyses demonstrated that archaeal consortia experienced a distinct shift and became stable after day 38. Methanomicrobiales and Methanosarcinales were the two predominant orders. Further investigations indicated that Methanolinea and Methanosaeta were responsible for methane production in the ADMD system. Hydrogenotrophic pathways might prevail over acetoclastic means for methanogenesis during the start-up, supported by specific methanogenic activity tests.
State Key Laboratory of Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Tongji University, Shanghai, PR China.
Hongguang
H
Yu
State Key Laboratory of Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Tongji University, Shanghai, PR China.
Qiaoying
Q
Wang
State Key Laboratory of Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Tongji University, Shanghai, PR China.
Zhiwei
Z
Wang
Istanbul Medeniyet University, Bioengineering Department, Kadıköy, Istanbul, Turkey.
Erkan
E
Sahinkaya
Laboratory of Polymères, Biopolymères and Surfaces, UMR 6270, University of Rouen-CNRS-INSA, Boulevard Maurice de Broglie, Mont-Saint-Aignan, France.
Yongli
Y
Li
State Key Laboratory of Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Tongji University, Shanghai, PR China.
Jinxing
J
Ma
State Key Laboratory of Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Tongji University, Shanghai, PR China.
Zhichao
Z
Wu
D019149:Bioreactors / Q000382:microbiology*; D008697:Methane / Q000378:metabolism; D017018:Methanomicrobiales / Q000302:isolation & purification*; D019640:Methanosarcinales / Q000302:isolation & purification*; D020560:Proteobacteria / Q000302:isolation & purification*; D012722:Sewage / Q000382:microbiology*
D012722:Sewage; D008697:Methane