Monitoring caries risks before the window of infection and later caries increment: a caries prediction study on rapid detection of Streptococcus mutans using monoclonal antibodies.
A new semi-quantitative enumeration system has been developed for the detection of Streptococcus mutans in saliva. Using two kinds of species-specific monoclonal antibodies, this system can quickly detect salivary S. mutans within 30 min and classify the results into three levels. The aim of this study was to evaluate the potential of this test kit in determining risk for the development of caries. Saliva samples collected during a compulsory dental examination from 56 children aged 18-months were tested. The children were classified into 3 groups according to the level of salivary S. mutans determined. After 18 months, 36 of the children underwent a second examination to investigate whether there was a correlation between salivary S. mutans level at the first examination and subsequent increment in caries. The results showed a good correlation between the two. This indicates that salivary S. mutans level before the window of infection is an indicator of caries risk and can be quickly detected using this test kit. This rapid test should be particularly useful in assessing risk of future caries in very young children.
Department of Social Dentistry, Tokyo Dental College.
Yoshinobu
Y
Maki
Takaharu
T
Sakayori
SoIchiro
S
Hirata
Takuo
T
Ishii
Atsushi
A
Tachino
D000911:Antibodies, Monoclonal / Q000276:immunology*; D058491:Bacterial Load; D004245:DMF Index; D003731:Dental Caries / Q000209:etiology* / Q000382:microbiology; D003733:Dental Caries Susceptibility / Q000502:physiology*; D005260:Female; D005500:Follow-Up Studies; D005544:Forecasting; D006801:Humans; D007223:Infant; D008297:Male; D011933:Reagent Kits, Diagnostic; D018570:Risk Assessment; D012463:Saliva / Q000382:microbiology*; D013045:Species Specificity; D013295:Streptococcus mutans / Q000276:immunology / Q000302:isolation & purification*
D000911:Antibodies, Monoclonal; D011933:Reagent Kits, Diagnostic