Characterization of human papillomavirus 6b L1 virus-like particles isolated from silkworms using capillary zone electrophoresis.
Human papillomavirus 6b L1 virus-like particles (VLPs) were successfully expressed using Bombyx mori nucleopolyhedrovirus (BmNPV) bacmid expression system and rapidly purified using size exclusion chromatography after ultracentrifugation procedure and characterized by capillary zone electrophoresis (CZE). The average capillary electrophoresis migration time was 11 min with the relative standard deviation (RSD) of 0.3% of human papillomavirus 6b L1 VLPs. After this threefold fractionation, the CZE samples were still further investigated by dynamic light scattering and immuno blotting. The versatile technique, CZE not only proved to be a valuable tool for VLP characterization, but was also found to be reliable and precise. Thus CZE will also be an important option for the quality control of VLPs in pharmaceutical research level.
Institute of Medicinal and Pharmaceutical Chemistry, Technische Universität Braunschweig, Beethovenstr. 55, 38106 Braunschweig, Germany.
Thomas
T
Hahne
Laboratory of Biotechnology, Department of Bioscience, Graduate School of Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan.
Muthukutty
M
Palaniyandi
Laboratory of Biotechnology, Department of Bioscience, Graduate School of Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan.
Tatsuya
T
Kato
Institute of Food Chemistry, Technische Universität Braunschweig, Schleinitzstr. 20, 38106 Braunschweig, Germany.
Peter
P
Fleischmann
Institute of Medicinal and Pharmaceutical Chemistry, Technische Universität Braunschweig, Beethovenstr. 55, 38106 Braunschweig, Germany.
Hermann
H
Wätzig
Laboratory of Biotechnology, Department of Bioscience, Graduate School of Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan. Electronic address: acypark@icloud.com.
Enoch Y
EY
Park
D000818:Animals; D012831:Bombyx / Q000737:chemistry* / Q000166:cytology / Q000821:virology; D036022:Capsid Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D019075:Electrophoresis, Capillary; D015870:Gene Expression; D052163:Human papillomavirus 6 / Q000737:chemistry*; D006801:Humans; D017924:Nucleopolyhedroviruses / Q000235:genetics; D053918:Papillomavirus Vaccines / Q000096:biosynthesis*; D058425:Vaccines, Virus-Like Particle / Q000096:biosynthesis*
D036022:Capsid Proteins; D053918:Papillomavirus Vaccines; D058425:Vaccines, Virus-Like Particle
Capillary zone electrophoresis; Fractionation; Human papillomavirus 6b L1; Silkworm; Size exclusion chromatography; Virus-like particles