Structure and biological activities of a pectic polysaccharide from Mosla chinensis Maxim. cv. Jiangxiangru.
A water-soluble pectic polysaccharide (MP-A40) was isolated and purified from Mosla chinensis Maxim. cv. Jiangxiangru for the first time, with a molecular weight of 32,600Da. MP-A40 was comprised of 68.63% galacturonic acid and 13.05% neutral sugar. In addition, arabinose, galactose, rhamnose, mannose and glucose composed the neutral sugar in a relative ratio of 4.94, 3.07, 2.13, 1.62 and 1.29% of the dry weight of MP-A40, respectively. Structural characterization of MP-A40 was investigated by methylation analysis and 1D/2D NMR spectroscopy. From the results, the structure of MP-A40 was revealed as follows: 1,4-linked α-d-GalpA and 1,4-linked α-d-GalpA6Me interspersed with rare t-Araf (0.60%), t-Rhap (1.67%) and t-GalpA (10.15%). Esterification assay showed that about 32% of the carboxylic groups in GalA residues existed as methyl ester. In addition, MP-A40 could inhibit the growth of human leukemic cell line K562 and stimulate nitric oxide production from RAW 264.7 macrophages both in dose-dependent manners.
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, Jiangxi Province, China; Guelph Food Research Centre, Agriculture and Agri-Food Canada, Guelph N1G 5C9, Ontario, Canada. Electronic address: julieli1234@gmail.com.
Jing-En
JE
Li
Guelph Food Research Centre, Agriculture and Agri-Food Canada, Guelph N1G 5C9, Ontario, Canada. Electronic address: cuis@agr.gc.ca.
Steve W
SW
Cui
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, Jiangxi Province, China. Electronic address: spnie@ncu.edu.cn.
Shao-Ping
SP
Nie
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, Jiangxi Province, China. Electronic address: myxie@ncu.edu.cn.
Ming-Yong
MY
Xie
D000970:Antineoplastic Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D006603:Hexuronic Acids / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D007155:Immunologic Factors / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D019686:Lamiaceae / Q000737:chemistry*; D007938:Leukemia / Q000188:drug therapy; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D009569:Nitric Oxide / Q000276:immunology; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology
D000970:Antineoplastic Agents; D006603:Hexuronic Acids; D007155:Immunologic Factors; D011134:Polysaccharides; D009569:Nitric Oxide; C007819:galacturonic acid
Antitumor; Immunomodulatory activity; Mosla chinensis Maxim. cv. Jiangxiangru; NMR spectroscopy; Pectic polysaccharide; Structure