Conformational change and biocatalysis-triggered spectral shift of single Au nanoparticles.
Spectral shift of localized plasmon resonance scattering of guanine-rich DNA modified single Au nanoparticles is observed under a dark field microscope equipped with a spectrometer. The spectra continuously red-shift with the conformational change of the guanine-rich DNA upon associating with K(+), hemin and the biocatalytic growth of the polymer. The scattering spectrum of single nanoparticles is proved to be sensitive both to a subtle conformational change and the biocatalysis process. 20 mM K(+) or 100 μM H2O2 can trigger a detectable peak shift. The present study paves a new and efficient way to extract chemical information from micro/nanospace.
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China. wangkang@nju.edu.cn xhxia@nju.edu.cn.
Yun
Y
Zhao
Ya-Kai
YK
He
Jing
J
Zhang
Feng-Bin
FB
Wang
Kang
K
Wang
Xing-Hua
XH
Xia
D055162:Biocatalysis; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D020862:GC Rich Sequence; D006046:Gold / Q000737:chemistry*; D006427:Hemin / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D011188:Potassium / Q000737:chemistry; D013057:Spectrum Analysis
D006427:Hemin; D006046:Gold; D004247:DNA; D011188:Potassium