Expression, crystallization and preliminary X-ray crystallographic analysis of D-alanine-D-alanine ligase from OXA-23-producing Acinetobacter baumannii K0420859.
Acinetobacter baumannii causes bacteraemia, pneumonia, other respiratory-tract and urinary-tract infections in humans. OXA-23 carbapenemase-producing A. baumannii K0420859 (A. baumannii OXA-23) is resistant to carbapenem, a common antibacterial drug. To develop an efficient and novel antibacterial drug against A. baumannii OXA-23, D-alanine-D-alanine ligase, which is essential in bacterial cell-wall synthesis, is of interest. Here, the D-alanine-D-alanine ligase (AbDdl) gene from A. baumannii OXA-23 was cloned and expressed, and the AbDdl protein was purified and crystallized; this enzyme can be used as a novel target for an antibacterial drug against A. baumannii OXA-23. The AbDdl crystal diffracted to a resolution of 2.8 Å and belonged to the orthorhombic space group P212121, with unit-cell parameters a = 113.4, b = 116.7, c = 176.5 Å, a corresponding VM of 2.8 Å(3) Da(-1) and a solvent content of 56.3%, and six protomers in the asymmetric unit.
Department of Biological Sciences, Konkuk University, Hwayang dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
Kim-Hung
KH
Huynh
Department of Advanced Technology Fusion, Konkuk University, Hwayang dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
Huyen-Thi
HT
Tran
Department of Advanced Technology Fusion, Konkuk University, Hwayang dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
Tan-Viet
TV
Pham
Department of Biological Sciences, Konkuk University, Hwayang dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
Ho-Phuong-Thuy
HP
Ngo
Marine Biotechnology Research Division, Korea Institute of Ocean Science and Technology, Ansan 426-744, Republic of Korea.
Sun-Shin
SS
Cha
Department of Microbiology and Immunology, Chonbuk National University Medical School, Jeonju, Jeonbuk 561-756, Republic of Korea.
Kyung Min
KM
Chung
National Leading Research Laboratory Department of Biological Sciences, Myongji University, 116 Myongjiro, Yongin, Gyeonggido 449-728, Republic of Korea.
Sang Hee
SH
Lee
Department of Biological Sciences, Konkuk University, Hwayang dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
Lin-Woo
LW
Kang
D040981:Acinetobacter baumannii / Q000201:enzymology*; D003460:Crystallization / Q000379:methods*; D018360:Crystallography, X-Ray / Q000379:methods*; D010453:Peptide Synthases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D001618:beta-Lactamases / Q000378:metabolism*
D011994:Recombinant Proteins; C466679:beta-lactamase OXA-23; D001618:beta-Lactamases; D010453:Peptide Synthases; C019638:D-alanylalanine synthetase
OXA-23-producing Acinetobacter baumannii; cell-wall synthesis; d-alanine-d-alanine ligase