Delivery of endocytosed proteins to the cell-division plane requires change of pathway from recycling to secretion.
Membrane trafficking is essential to fundamental processes in eukaryotic life, including cell growth and division. In plant cytokinesis, post-Golgi trafficking mediates a massive flow of vesicles that form the partitioning membrane but its regulation remains poorly understood. Here, we identify functionally redundant Arabidopsis ARF guanine-nucleotide exchange factors (ARF-GEFs) BIG1-BIG4 as regulators of post-Golgi trafficking, mediating late secretion from the trans-Golgi network but not recycling of endocytosed proteins to the plasma membrane, although the TGN also functions as an early endosome in plants. In contrast, BIG1-4 are absolutely required for trafficking of both endocytosed and newly synthesized proteins to the cell-division plane during cytokinesis, counteracting recycling to the plasma membrane. This change from recycling to secretory trafficking pathway mediated by ARF-GEFs confers specificity of cargo delivery to the division plane and might thus ensure that the partitioning membrane is completed on time in the absence of a cytokinesis-interphase checkpoint. DOI: http://dx.doi.org/10.7554/eLife.02131.001.
Department of Developmental Genetics, The Center for Plant Molecular Biology (ZMBP), University of Tübingen, Tübingen, Germany.
Sandra
S
Richter
Marika
M
Kientz
Sabine
S
Brumm
Mads Eggert
ME
Nielsen
Misoon
M
Park
Richard
R
Gavidia
Cornelia
C
Krause
Ute
U
Voss
Hauke
H
Beckmann
Ulrike
U
Mayer
York-Dieter
YD
Stierhof
Gerd
G
Jürgens
D017360:Arabidopsis / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D002455:Cell Division; D004705:Endocytosis; D006056:Golgi Apparatus / Q000378:metabolism; D021381:Protein Transport
D029681:Arabidopsis Proteins
ARF-GEF; cell division; gegulation of vesicle traffic; post-Golgi trafficking; recycling; secretion