An anterograde rabies virus vector for high-resolution large-scale reconstruction of 3D neuron morphology.
Glycoprotein-deleted rabies virus (RABV ∆G) is a powerful tool for the analysis of neural circuits. Here, we demonstrate the utility of an anterograde RABV ∆G variant for novel neuroanatomical approaches involving either bulk or sparse neuronal populations. This technology exploits the unique features of RABV ∆G vectors, namely autonomous, rapid high-level expression of transgenes, and limited cytotoxicity. Our vector permits the unambiguous long-range and fine-scale tracing of the entire axonal arbor of individual neurons throughout the brain. Notably, this level of labeling can be achieved following infection with a single viral particle. The vector is effective over a range of ages (>14 months) aiding the studies of neurodegenerative disorders or aging, and infects numerous cell types in all brain regions tested. Lastly, it can also be readily combined with retrograde RABV ∆G variants. Together with other modern technologies, this tool provides new possibilities for the investigation of the anatomy and physiology of neural circuits.
Physiopathologie de la plasticité neuronale, INSERM, Neurocentre Magendie, U862, Bordeaux, France.
Matthias Georg
MG
Haberl
Silvia
S
Viana da Silva
Jason M
JM
Guest
Melanie
M
Ginger
Alexander
A
Ghanem
Christophe
C
Mulle
Marcel
M
Oberlaender
Karl-Klaus
KK
Conzelmann
Andreas
A
Frick
D000544:Alzheimer Disease / Q000378:metabolism / Q000473:pathology; D000818:Animals; D001370:Axonal Transport / Q000502:physiology; D001921:Brain / Q000166:cytology* / Q000378:metabolism / Q000473:pathology; D004195:Disease Models, Animal; D005822:Genetic Vectors / Q000378:metabolism*; D006023:Glycoproteins / Q000378:metabolism; D021621:Imaging, Three-Dimensional / Q000379:methods*; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000166:cytology* / Q000378:metabolism / Q000473:pathology; D011820:Rabies virus / Q000235:genetics* / Q000378:metabolism; D013194:Staining and Labeling / Q000379:methods*
D006023:Glycoproteins