Improvement production of bacterial cellulose by semi-continuous process in molasses medium.
Bacterial cellulose (BC) has unique properties such as structural, functional, physical and chemical. The mass production of BC for industrial application has recently become attractive to produce more economical and high productive cellulose. In this study, to improve the productivity of bacterial cellulose (BC), BC production by Gluconacetobacter xylinus FC01 was investigated in molasses medium with static semi-continuous operation mode. Cell dry weight, polysaccharide, sugar and cellulose concentrations were monitored and cellulose was characterized by Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM). The highest cellulose yield (1.637 g/L) was obtained in SCP50-7d, which molasses of 1/2 ratio for 7 days by static semi-continuous operation mode. The results show that BC can be highly produced by G. xylinus in molasses with static semi-continuous process than batch process. We claimed that low-cost medium with semi-continuous operation mode in static culture is a good candidate for industrial scale BC productions.
Department of Genetic and Bioengineering, Faculty of Engineering and Architecture, Yeditepe University, Ataşehir, 34755 İstanbul, Turkey.
Fatih
F
Cakar
Department of Genetic and Bioengineering, Faculty of Engineering and Architecture, Yeditepe University, Ataşehir, 34755 İstanbul, Turkey.
Işılay
I
Ozer
Department of Genetic and Bioengineering, Faculty of Engineering and Architecture, Yeditepe University, Ataşehir, 34755 İstanbul, Turkey. Electronic address: ali.aytekin@yeditepe.edu.tr.
A Özhan
AÖ
Aytekin
Department of Genetic and Bioengineering, Faculty of Engineering and Architecture, Yeditepe University, Ataşehir, 34755 İstanbul, Turkey.
Fikrettin
F
Sahin
D019149:Bioreactors; D002482:Cellulose / Q000737:chemistry / Q000378:metabolism*; D003470:Culture Media; D005285:Fermentation; D041822:Gluconacetobacter xylinus / Q000201:enzymology*; D008855:Microscopy, Electron, Scanning; D008965:Molasses / Q000032:analysis*; D017550:Spectroscopy, Fourier Transform Infrared
D003470:Culture Media; D002482:Cellulose
Bacterial cellulose; Gluconacetobacter xylinus; Molasses; Semi-continuous process