Utilization of banana peel as a novel substrate for biosurfactant production by Halobacteriaceae archaeon AS65.
In this study, biosurfactant-producing bacteria was evaluated for biosurfactant production by using banana peel as a sole carbon source. From the 71 strains screened, Halobacteriaceae archaeon AS65 produced the highest biosurfactant activity. The highest biosurfactant production (5.30 g/l) was obtained when the cells were grown on a minimal salt medium containing 35 % (w/v) banana peel and 1 g/l commercial monosodium glutamate at 30 °C and 200 rpm after 54 h of cultivation. The biosurfactant obtained by extraction with ethyl acetate showed high surface tension reduction (25.5 mN/m), a small critical micelle concentration value (10 mg/l), thermal and pH stability with respect to surface tension reduction and emulsification activity, and a high level of salt tolerance. The biosurfactant obtained was confirmed as a lipopeptide by using a biochemical test FT-IR, NMR, and mass spectrometry. The crude biosurfactant showed a broad spectrum of antimicrobial activity and had the ability to emulsify oil, enhance PAHs solubility, and oil bioremediation.
Trang Campus/Faculty of Science and Fisheries Technology, Rajamangala University of Technology Srivijaya, Trang, Thailand.
Chanika Saenge
CS
Chooklin
Suppasil
S
Maneerat
Atipan
A
Saimmai
D000890:Anti-Infective Agents / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D001709:Biotechnology / Q000379:methods*; D002244:Carbon / Q000378:metabolism; D055598:Chemical Phenomena; D004785:Environmental Pollutants / Q000737:chemistry / Q000302:isolation & purification; D006216:Halobacteriaceae / Q000378:metabolism*; D054327:Lubricants / Q000737:chemistry / Q000302:isolation & purification; D008670:Metals / Q000737:chemistry / Q000302:isolation & purification; D028521:Musa / Q000737:chemistry*; D009584:Nitrogen / Q000378:metabolism; D011084:Polycyclic Aromatic Hydrocarbons / Q000737:chemistry / Q000302:isolation & purification; D013501:Surface-Active Agents / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D013997:Time Factors; D014867:Water / Q000737:chemistry
D000890:Anti-Infective Agents; D004785:Environmental Pollutants; D054327:Lubricants; D008670:Metals; D011084:Polycyclic Aromatic Hydrocarbons; D013501:Surface-Active Agents; D014867:Water; D002244:Carbon; D009584:Nitrogen