Assessment of the breakdown products of solar/UV induced photolytic degradation of food dye tartrazine.
The food dye tartrazine (CI 19140) was exposed to UV irradiation from an artificial source, a mercury vapor lamp, and a natural one, sunlight. It was observed that conditions such as energy dose, irradiation time, pH and initial dye concentration affected its discoloration. There was 100% of color removal, after 30min of irradiation, when a dye solution 1×10(-5)molL(-1) was submitted to an energy dose of 37.8Jcm(-2). Liquid Chromatography coupled to Diode Array Detection and Mass Spectrometry confirmed the cleavage of the chromophore group and the formation of five by-products at low concentration. Although by-products were formed, the Salmonella/microsome mutagenicity assay performed for both, the dye solution at a dose of 5.34 μg/plate [DOSAGE ERROR CORRECTED] and the solutions obtained after exposure to UV irradiation, did not present mutagenic activity for TA98 and TA100 with and without S9.
Chemistry Institute (Unesp), Department of Analytical Chemistry, Rua Francisco Degni 55, Bairro Quitandinha, 14800-900 Araraquara, Brazil. Electronic address: snts.tuane@gmail.com.
Tuane Cristina
TC
dos Santos
Chemistry Institute (Unesp), Department of Analytical Chemistry, Rua Francisco Degni 55, Bairro Quitandinha, 14800-900 Araraquara, Brazil.
Guilherme Julião
GJ
Zocolo
Technology Institute (Unicamp), Rua Paschoal Marmo 1888, 13484-332 Limeira, Brazil.
Daniel Alexandre
DA
Morales
Technology Institute (Unicamp), Rua Paschoal Marmo 1888, 13484-332 Limeira, Brazil.
Gisela de Aragão
Gde A
Umbuzeiro
Chemistry Institute (Unesp), Department of Analytical Chemistry, Rua Francisco Degni 55, Bairro Quitandinha, 14800-900 Araraquara, Brazil.
Maria Valnice Boldrin
MV
Zanoni
D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D005505:Food Coloring Agents / Q000737:chemistry* / Q000633:toxicity; D006863:Hydrogen-Ion Concentration; D009152:Mutagenicity Tests; D009153:Mutagens / Q000032:analysis / Q000633:toxicity; D010782:Photolysis; D012486:Salmonella typhimurium / Q000187:drug effects; D053719:Tandem Mass Spectrometry; D013645:Tartrazine / Q000737:chemistry* / Q000633:toxicity; D014466:Ultraviolet Rays
D005505:Food Coloring Agents; D009153:Mutagens; D013645:Tartrazine
Breakdown products; Dye photolysis; LC–MS/MS; Mutagenicity; Tartrazine