Genomic characterization of a bovine viral diarrhea virus 1 isolate from swine.
The SD0803 strain of the bovine viral diarrhea virus (BVDV) was isolated from a piglet in China in 2008 and has been classified as a novel subgenotype of BVDV-1. To describe the molecular features of this novel subgenotype, we sequenced and characterized the complete genome of the SD0803 virus. The genome is 12,271 bp in length and contains 5' and 3' untranslated regions (UTRs) that flank an open reading frame (ORF) encoding a 3,898-amino-acid polypeptide. The full-length genome of the SD0803 strain shares 78.8% to 83.3% identity with those of other BVDV-1 strains, 70.0% to 70.7% identity with those of BVDV-2 strains, and less than 67.6% identity with those of other pestiviruses. The highest level of shared identity was 83.3% between the complete SD0803 genome and that of the ZM-95 strain of BVDV-1. Phylogenetic analysis of the 5' UTR and the coding sequence for the N-terminal protease fragment of the SD0803 polyprotein indicated that the SD0803 virus is a member of the novel subgenotype BVDV-1q, isolates of which have been identified recently in dairy cattle and camels in China.
Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, 200241, China.
Yu
Y
Deng
Tong-Ling
TL
Shan
Wu
W
Tong
Xu-Chen
XC
Zheng
Yi-Yei
YY
Guo
Hao
H
Zheng
San-Jie
SJ
Cao
Xin-Tian
XT
Wen
Guang-Zhi
GZ
Tong
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D000818:Animals; D002681:China; D016000:Cluster Analysis; D030241:Diarrhea Virus 1, Bovine Viral / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D018182:Pestivirus Infections / Q000662:veterinary* / Q000821:virology; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013552:Swine / Q000821:virology*
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D012367:RNA, Viral