Application ofin vitropollination of opened ovaries to obtainBrassica oleraceaL. ×B. rapaL. hybrids.
This study presents the results of experiments concerning: (1) interspecific hybridization ofBrassica oleracea×Brassica rapavia application ofin vitroplacental pollination and (2) embryological analysis of the process of resynthesis ofBrassica napus. In order to overcome certain stigma/style barriers,B. rapapollen was placedin vitroon an openedB. oleraceaovary (with style removed). Pollinated ovaries were cultured on Murashige and Skoog (MS) medium. After 24-d culture, the developing embryos were isolated from immature seeds and transferred onto MS medium supplemented with 0.47 μM kinetin, 0.49 μM 1-naphthaleneacetic acid, and 10% (v/v) coconut water. When the embryos had turned green, they were immediately placed onto MS medium with 100 μM kinetin. After development of the seedling, plantlets were transferred to soil. Chromosome doubling was achieved after another week. Cytometric analysis of nuclear DNA confirmed the hybrid nature of the plants. Resynthesis ofB. napuscan be performed through interspecific hybridization ofB. oleracea×B. rapafollowed by embryo rescue and genome doubling.
Department of Genetics and Breeding of Oilseed Crops, Plant Breeding and Acclimatization Institute - National Research Institute, Strzeszynska 36, 60-479 Poznan, Poland.
Katarzyna
K
Sosnowska
Department of Genetics and Breeding of Oilseed Crops, Plant Breeding and Acclimatization Institute - National Research Institute, Strzeszynska 36, 60-479 Poznan, Poland.
Teresa
T
Cegielska-Taras
Brassica oleracea; Brassica rapa; In vitro pollination; Interspecific hybridization