Extraction optimization and antinociceptive activity of (1→3)-β-d-glucan from Rhodotorulamucilaginosa.
β-d-glucans are polymers of d-glucose monomers found in the cell walls of many bacteria, plants, fungi and yeasts. A variety of β-d-glucans differing in structures have been isolated from various sources and their biological activity to be regulated by various structural factors, such as the primary structure, molecular weight, solubility, and conformation. This study investigated the effect of extraction time and temperature on the yield of β-d-glucan produced by Rhodotorulamucilaginosa. A statistical Doehlert design was applied to determine the important effects and interactions of these independent variables on the yield of β-d-glucan, the dependent variable. Significant models were obtained. The best yield was of 25% obtained after 128min of extraction in a temperature of 72°C. The polysaccharides were characterized as (1⟶3)-β-d-glucan by methods spectroscopic (FT-IR, (1)HNMR and (13)CNMR). In addition, the antinociceptive effect was evaluated using different experimental tests (acetic acid-induced writhing test, formalin test and tail immersion test). The (1⟶3)-β-d-glucan showed a potent peripheral antinociceptive effect, possibly by the inhibition of inflammatory mediators.
Laboratório de Enzimologia e Tecnologia de Fermentação; Departamento de Saúde; Universidade Estadual de Feira de Santana (UEFS), Bahia, Brazil.
Gildomar L
GL
Valasques Junior
Departamento de Saúde; Universidade Estadual de Feira de Santana (UEFS), Bahia, Brazil.
Flávia O
FO
de Lima
Departamento de Química Orgânica, Instituto de Química, Universidade Federal da Bahia,Campus Universitário de Ondina, 40170-115 Salvador, BA, Brazil.
Elisangela F
EF
Boffo
Departamento de Saúde; Universidade Estadual de Feira de Santana (UEFS), Bahia, Brazil.
Jener D G
JD
Santos
Departamento de Saúde; Universidade Estadual de Feira de Santana (UEFS), Bahia, Brazil.
Bruno C
BC
da Silva
Laboratório de Enzimologia e Tecnologia de Fermentação; Departamento de Saúde; Universidade Estadual de Feira de Santana (UEFS), Bahia, Brazil. Electronic address: sandrinhaassis@yahoo.com.br.
Sandra A
SA
de Assis
D000700:Analgesics / Q000737:chemistry* / Q000302:isolation & purification / Q000627:therapeutic use*; D000818:Animals; D005591:Chemical Fractionation; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D051379:Mice; D010146:Pain / Q000188:drug therapy*; D012248:Rhodotorula / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D047071:beta-Glucans / Q000737:chemistry* / Q000302:isolation & purification / Q000627:therapeutic use*
D000700:Analgesics; D047071:beta-Glucans
Antinociceptive; Optimization; Response surface methodology; Rhodotorulamucilaginosa; β-d-glucan