Isolation, characterization and antioxidant activity of polysaccharide from Schisandra sphenanthera.
A water-soluble polysaccharide (SSPP11) from Schisandra sphenanthera was purified by DEAE-cellulose and Sephadex G-100 columns. Structure of SSPP11 and its antioxidant activity was evaluated. Results showed that SSPP11 has a molecular weight of 5.3×10(3)Da and is composed of Man, Glu and Gal. A linkage analysis and NMR study revealed that SSPP11 has a backbone of →1)-d-Man-(6→, →1)-d-Manp-(2→, →1)-d-Glup(4→, →1)-d-Glup-(6→, →1)-d-Galp-(4→, →1)-d-Galp-(4,6→ and →1)-d-Manp-(3,6→, with Man, Glu and Gal, which are distributed in branched chains. The Congo red absorption test revealed that SSPP11 has a triple helix stereo-configuration. Moreover, antioxidant activity of SSPP11 was stronger than the polysaccharide from Schisandra chinensis (Turcz.) Baill. In sum, this study demonstrates that a moderate molecular weight, triple helix stereo-configuration and higher degree of branching are beneficial for exerting antioxidant activity.
School of Chemistry and Chemical Engineering, Jiangsu University, Xuefu Rd. 301, Zhenjiang 212013, China.
Ting
T
Zhao
School of Food and Biological Engineering, Jiangsu University, Xuefu Rd. 301, Zhenjiang 212013, Jiangsu, China.
Guanghua
G
Mao
School of Food and Biological Engineering, Jiangsu University, Xuefu Rd. 301, Zhenjiang 212013, Jiangsu, China.
Weiwei
W
Feng
Nucell Biotechnology Co. Ltd., Jingsi Rd. 2, Zhenjiang 212009, Jiangsu, China.
Riwen
R
Mao
School of Chemistry and Chemical Engineering, Jiangsu University, Xuefu Rd. 301, Zhenjiang 212013, China.
Xiaoyun
X
Gu
School of Chemistry and Chemical Engineering, Jiangsu University, Xuefu Rd. 301, Zhenjiang 212013, China.
Ting
T
Li
School of Pharmacy, Jiangsu University, Xuefu Rd. 301, Zhenjiang 212013, Jiangsu, China.
Qian
Q
Li
School of Chemistry and Chemical Engineering, Jiangsu University, Xuefu Rd. 301, Zhenjiang 212013, China.
Yongtuan
Y
Bao
School of Chemistry and Chemical Engineering, Jiangsu University, Xuefu Rd. 301, Zhenjiang 212013, China. Electronic address: yangliuqing@ujs.edu.cn.
Liuqing
L
Yang
School of the Environment, Jiangsu University, Xuefu Rd. 301, Zhenjiang 212013, Jiangsu, China. Electronic address: wuxy@ujs.edu.cn.
Xiangyang
X
Wu
D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D016166:Free Radical Scavengers / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D032141:Schisandra
D000975:Antioxidants; D016166:Free Radical Scavengers; D010936:Plant Extracts; D011134:Polysaccharides
Antioxidant activity; Methylation analysis; NMR spectroscopy; Polysaccharide; Schisandra sphenanthera Rehd. et Wits.; Structural elucidation