1-Deoxynojirimycin attenuates high glucose-accelerated senescence in human umbilical vein endothelial cells.
The influence of 1-deoxynojirimycin (DNJ) derived from mulberry on senescence of endothelial cells was examined with the goal of discovery of a method for prevention of senescence of blood vessels. The effect of DNJ on senescence of human umbilical vein endothelial cells (HUVECs) promoted under high glucose condition was determined. HUVECs were cultured in normal glucose (5.6mmol/L, NG group), normal glucose plus DNJ (10μmol/L, DNJ group), high glucose (30mmol/L, HG group), or high glucose plus DNJ (10μmol/L, HG+DNJ group) and passaged until they reached senescence. The proliferation rate was markedly decreased in the HG group compared with the NG group, and this phenomenon was reversed by DNJ. The frequency of senescent (SA-ß-Gal-positive) cells and the expression level of senescence genes (PAI-1 and p21) were significantly higher in the HG group compared with the NG group, and these changes were blocked by DNJ. Monocyte adhesion, NF-kB activity, and reactive oxygen species production, all of which promote cellular senescence, were significantly increased in the HG group compared with the NG group, and again these changes were blocked by DNJ. Therefore, these results show that DNJ delays cellular senescence that is promoted under high glucose condition.
Laboratory of Food and Biomolecular Science, Graduate School of Agriculture, Tohoku University, Sendai 981-8555, Japan.
Shuang
S
E
Laboratory of Food and Biomolecular Science, Graduate School of Agriculture, Tohoku University, Sendai 981-8555, Japan.
Ryo
R
Kijima
Laboratory of Food and Biomolecular Science, Graduate School of Agriculture, Tohoku University, Sendai 981-8555, Japan.
Taro
T
Honma
Laboratory of Food and Biomolecular Science, Graduate School of Agriculture, Tohoku University, Sendai 981-8555, Japan.
Kazushi
K
Yamamoto
Laboratory of Food and Biomolecular Science, Graduate School of Agriculture, Tohoku University, Sendai 981-8555, Japan.
Yu
Y
Hatakeyama
Laboratory of Food and Biomolecular Science, Graduate School of Agriculture, Tohoku University, Sendai 981-8555, Japan.
Yasuna
Y
Kitano
NARO Agricultural Research Center, Tsukuba 305-8666, Japan.
Toshiyuki
T
Kimura
Food and Biodynamic Chemistry Laboratory, Graduate School of Agriculture, Tohoku University, Sendai 981-8555, Japan.
Kiyotaka
K
Nakagawa
Food and Biodynamic Chemistry Laboratory, Graduate School of Agriculture, Tohoku University, Sendai 981-8555, Japan.
Teruo
T
Miyazawa
Laboratory of Food and Biomolecular Science, Graduate School of Agriculture, Tohoku University, Sendai 981-8555, Japan. Electronic address: tsuzukit@biochem.tohoku.ac.jp.
Tsuyoshi
T
Tsuduki
D017485:1-Deoxynojirimycin / Q000008:administration & dosage / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000187:drug effects*; D018920:Coculture Techniques; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical / Q000379:methods; D005947:Glucose / Q000008:administration & dosage / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D006801:Humans; D009000:Monocytes / Q000187:drug effects / Q000502:physiology; D016328:NF-kappa B / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism
D016328:NF-kappa B; D017382:Reactive Oxygen Species; D017485:1-Deoxynojirimycin; D005947:Glucose
1-Deoxynojirimycin; DNJ; HUVEC; High glucose; Mulberry; Senescence