Hormonal and metabolic effects of polyunsaturated fatty acid (omega-3) on polycystic ovary syndrome induced rats under diet.
OBJECTIVE
PCOS (polycystic ovary syndrome) produces symptoms in approximately 5% to 10% of women of reproductive age (12-45 years old). It is thought to be one of the leading causes of female subfertility. This study aimed to confirm the role of nutrition containing omega-3 (polyunsaturated fatty acid) on control of experimental PCO induced by estradiol-valerat in rats.


METHODS
Wistar female rats (n=40) were allocated into control (n=10) and test groups (n= 30), test group was subdivided into 3 groups: G1, received omega-3 (240 mg/kg/orally/daily); G2 and G3 groups were induced PCO by single injection of estradiol-valerate (16 mg/kg/IM). Group 3 received omega-3 (240 mg/kg/orally/daily) and low carbohydrate feeding for 60 subsequent days; on sixtieth day 5 ml blood samples and ovarian tissues of all rats in the group were removed and prepared for biochemical and hormonal analysis.


RESULTS
Catalase, GPX (Glutathione peroxidase), SOD (Superoxide dismutase) in groups that received omega-3 showed higher levels, but MDA (malondialdehyde) level was significantly decreased (P<0.05) in comparison with other experimental groups. Ovarian weights in both experimental and control groups were similar (P<0.05). Level of serum FSH (follicle stimulating hormone) was decreased, but level of testosterone was significantly increased (P<0.05) in PCO group in comparison with control and omega-3 groups.


CONCLUSIONS
RESULTS revealed that administration of omega-3 plus lower carbohydrate food significantly controlled   PCO syndrome and balanced FSH and testosterone.
Women's Reproductive Health Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Elaheh
E
Ouladsahebmadarek
Department of Pathology, Tabriz Branch, Islamic Azad University, Tabriz, Iran.
Arash
A
Khaki
Women's Reproductive Health Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Sharareh
S
Khanahmadi
Department of Biochemistry, Islamic Azad University, Pharmaceutical, Tehran, Iran.
Hamidreza
H
Ahmadi Ashtiani
Women's Reproductive Health Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Pooya
P
Paknejad
Department of Pathology, Tabriz Branch, Islamic Azad University, Tabriz, Iran.
Mohammad Reza
MR
Ayubi
Catalaze (CAT); Follicle stimulating hormone (FSH); Glutathione peroxidase (GPX); Omega-3; Polycystic ovary (PCO); Super oxide dismutase (SOD); Testosterone