Phylogenetic analysis of the GST family in Anopheles (Nyssorhynchus) darlingi.
Anopheles darlingi Root, 1926 and Anopheles gambiae (Diptera: Culicidae) are the most important human malaria vectors in South America and Africa, respectively. The two species are estimated to have diverged 100 million years ago. Studies on the phylogenetics and evolution of gene sequences, such as glutathione S-transferase (GST) in disease-transmitting mosquitoes are scarce. The sigma class GST (KC890767) from the transcriptome of An. darlingi captured in the Brazilian Amazon was studied by in silico hybridization, and mapped to chromosome 3 of An. gambiae. The sigma class GST of An. darlingi was used for phylogenetic analyses to understand the GST base composition of the most recent common ancestor between An. darlingi, Anopheles gambiae, Aedes aegypti and Culex quinquefasciatus. The GST (KC890767) of An. darlingi was studied to generate the main divergence branches using a Neighbor-Joining and bootstrapping approaches to confirm confidence levels on the tree nodes that separate the An. darlingi and other mosquito species. The results showed divergence between An. gambiae, Ae. Aegypti, Cx. quinquefasciatus, and Phlebotomus papatasi as outgroup, and the homology relationship between sigma class GST of An. darlingi and GSTS1_1 gene of An. gambiae was valuable for phylogenetic and evolutionary studies.
Programa de Pós-Graduação em Genética, Conservação e Biologia Evolutiva-PPG GCBEv, Instituto Nacional de Pesquisas da Amazônia-INPA, Manaus, Amazonas, Brazil.
Gilson Martins de
GM
Azevedo-Júnior
Programa de Pós-Graduação em Genética, Conservação e Biologia Evolutiva-PPG GCBEv, Instituto Nacional de Pesquisas da Amazônia-INPA, Manaus, Amazonas, Brazil.
Giselle Moura
GM
Guimarães-Marques
Programa de Pós-Graduação em Genética, Conservação e Biologia Evolutiva-PPG GCBEv, Instituto Nacional de Pesquisas da Amazônia-INPA, Manaus, Amazonas, Brazil.
Leticia
L
Cegatti Bridi
Programa de Pós-Graduação em Biotecnologia e Recursos Naturais-PPG MBT, Universidade do Estado do Amazonas-UEA, Manaus, Amazonas, Brazil.
Ketlen
K
Christine Ohse
Laboratório de Biologia de Sistemas, Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas-UNICAMP, Campinas, São Paulo, Brazil.
Renato
R
Vicentini
Coordenação de Sociedade, Ambiente e Saúde-CSAS, Laboratório de Vetores da Malária e Dengue/INPA, Manaus, Amazonas, Brazil.
Wanderli
W
Tadei
Coordenação de Sociedade, Ambiente e Saúde-CSAS, Laboratório de Vetores da Malária e Dengue/INPA, Manaus, Amazonas, Brazil. Electronic address: msrafael@inpa.gov.br.
Míriam Silva
MS
Rafael
D000595:Amino Acid Sequence; D000818:Animals; D000852:Anopheles / Q000235:genetics / Q000378:metabolism*; D020224:Expressed Sequence Tags; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D005810:Multigene Family; D010802:Phylogeny
D005982:Glutathione Transferase
Anopheles darlingi; Glutathione S-transferase; Sigma class