Proteomic characterization of seeds from yellow lupin (Lupinus luteus L.).
Yellow lupin (Lupinus luteus L.) is a legume crop containing a large amount of protein in its seeds. In this study, we constructed a seed-protein catalog to provide a foundation for further study of the seeds. A total of 736 proteins were identified in 341 2DE spots by nano-LC-MS/MS. Eight storage proteins were found as multiple spots in the 2DE gels. The 736 proteins correspond to 152 unique proteins as shown by UniRef50 clustering. Sixty-seven of the 152 proteins were associated with KEGG-defined pathways. Of the remaining proteins, 57 were classified according to a GO term. The functions of the remaining 28 proteins have yet to be determined. This is the first yellow lupin seed-protein catalog, and it contains considerably more data than previously reported for white lupin (L. albus L.).
Agriaquaculture Nutritional Genomic Center, CGNA, Genomics and Bioinformatics Unit, Temuco, Chile.
Takahiro
T
Ogura
Jun
J
Ogihara
Michio
M
Sunairi
Hidetaka
H
Takeishi
Tomoko
T
Aizawa
Marcos R
MR
Olivos-Trujillo
Iván J
IJ
Maureira-Butler
Haroldo E
HE
Salvo-Garrido
D002853:Chromatography, Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D031297:Lupinus / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D012639:Seeds / Q000737:chemistry / Q000378:metabolism*; D053719:Tandem Mass Spectrometry
D010940:Plant Proteins; D020543:Proteome
2DE; Legume seed; Lupinus luteus L; Nano-LC-MS/MS; Plant proteomics