Catalpol provides protective effects against cerebral ischaemia/reperfusion injury in gerbils.
OBJECTIVE
To investigate the protective effect of catalpol on cerebral ischaemia/reperfusion (CI/R) injury in gerbils and further explore the underlying mechanism.


METHODS
A gerbil model of CI/R was prepared by bilateral common carotid occlusion for 10 min followed by 6 h reperfusion. Catalpol (5, 10 or 20 mg/kg per day) was injected intraperitoneally for 3 days before the carotid occlusion. Stroke index was measured during the reperfusion. The contents of endogenous neuropeptides, endothelin-1 (ET-1) and calcitonin gene-related peptide in plasma were evaluated by radioimmunoassay. Superoxide dismutase (SOD) and malondialdehyde (MDA) in brain tissue homogenate were also examined.


RESULTS
The results showed that catalpol significantly improved the stroke index compared with CI/R control group (P < 0.05 or P < 0.01). Catalpol significantly increased the activity of SOD at the doses of 10 and 20 mg/kg (P ≤ 0.05), decreased the brain MDA content and the plasma level of ET-1 at the doses of 10 and 20 mg/kg (P ≤ 0.01).


CONCLUSIONS
These data suggested that the efficacy of catalpol pretreatment on CI/R injury may be attributed to reduction of free radicals and inhibition of lipid peroxidation and ET-1 production.
Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
Yan-Ru
YR
Liu
Peng-Wei
PW
Li
Jian-Jun
JJ
Suo
Yan
Y
Sun
Bo-Ai
BA
Zhang
Hong
H
Lu
Hong-Can
HC
Zhu
Guo-Bin
GB
Zhang
D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D002545:Brain Ischemia / Q000188:drug therapy* / Q000378:metabolism; D015740:Calcitonin Gene-Related Peptide / Q000097:blood; D002544:Cerebral Infarction / Q000188:drug therapy* / Q000378:metabolism; D004195:Disease Models, Animal; D019332:Endothelin-1 / Q000097:blood; D005260:Female; D005609:Free Radicals / Q000378:metabolism; D005849:Gerbillinae; D007274:Injections, Intraperitoneal; D057888:Iridoid Glucosides / Q000494:pharmacology / Q000627:therapeutic use*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009479:Neuropeptides / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D032267:Rehmannia / Q000737:chemistry; D015427:Reperfusion Injury / Q000097:blood / Q000378:metabolism / Q000517:prevention & control*; D020521:Stroke / Q000517:prevention & control*; D013482:Superoxide Dismutase / Q000378:metabolism
D019332:Endothelin-1; D005609:Free Radicals; D057888:Iridoid Glucosides; D009479:Neuropeptides; D010936:Plant Extracts; C078040:catalpol; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D015740:Calcitonin Gene-Related Peptide
catalpol; cerebral ischaemia/reperfusion injury; endothelin-1; malondialdehyde; superoxide dismutase