Systems level approach reveals the correlation of endoderm differentiation of mouse embryonic stem cells with specific microstructural cues of fibrin gels.
Stem cells receive numerous cues from their associated substrate that help to govern their behaviour. However, identification of influential substrate characteristics poses difficulties because of their complex nature. In this study, we developed an integrated experimental and systems level modelling approach to investigate and identify specific substrate features influencing differentiation of mouse embryonic stem cells (mESCs) on a model fibrous substrate, fibrin. We synthesized a range of fibrin gels by varying fibrinogen and thrombin concentrations, which led to a range of substrate stiffness and microstructure. mESCs were cultured on each of these gels, and characterization of the differentiated cells revealed a strong influence of substrate modulation on gene expression patterning. To identify specific substrate features influencing differentiation, the substrate microstructure was quantified by image analysis and correlated with stem cell gene expression patterns using a statistical model. Significant correlations were observed between differentiation and microstructure features, specifically fibre alignment. Furthermore, this relationship occurred in a lineage-specific manner towards endoderm. This systems level approach allows for identification of specific substrate features from a complex material which are influential to cellular behaviour. Such analysis may be effective in guiding the design of scaffolds with specific properties for tissue engineering applications.
Department of Chemical Engineering, University of Pittsburgh, , Pittsburgh, PA, USA.
Keith
K
Task
Antonio
A
D'Amore
Satish
S
Singh
Joe
J
Candiello
Maria
M
Jaramillo
William R
WR
Wagner
Prashant
P
Kumta
Ipsita
I
Banerjee
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D019070:Cell Lineage; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism*; D004707:Endoderm / Q000166:cytology / Q000378:metabolism*; D005337:Fibrin / Q000737:chemistry*; D005782:Gels / Q000737:chemistry*; D051379:Mice; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds
D005782:Gels; D005337:Fibrin
differentiation; embryonic stem cells; fibrin substrate; microstructural topology; regression analysis; systems level modelling