Suppression of IL-10 production by calcitriol in patients with multiple sclerosis.
We investigated whether calcitriol (1,25-dihydroxyvitamin D) differentially modulates cytokine production by peripheral blood mononuclear cells from multiple sclerosis (MS) patients compared with that from healthy controls. In response to phytohemagglutinin (PHA) or lipopolysaccharide (LPS), cytokine level in a calcitriol-added sample was normalized to that in a calcitriol-absent sample, and this relative unit was compared. The relative unit of IL-12/23(p40) in LPS-stimulation was higher in MS patients. Moreover, the relative unit of IL-10 in PHA-stimulation was lower in MS patients, and negatively correlated with the Expanded Disability Status Scale. The anti-inflammatory response to vitamin D may be reduced in MS.
Department of Clinical Research, Hokkaido Medical Center, Sapporo, Japan. Electronic address: niino@hok-mc.hosp.go.jp.
Masaaki
M
Niino
Sapporo Neurology Clinic, Sapporo, Japan.
Toshiyuki
T
Fukazawa
Department of Clinical Research, Hokkaido Medical Center, Sapporo, Japan; Department of Neurology, Hokkaido Medical Center, Sapporo, Japan.
Yusei
Y
Miyazaki
Department of Clinical Research, Hokkaido Medical Center, Sapporo, Japan.
Eri
E
Takahashi
Department of Neurology, Hokkaido Medical Center, Sapporo, Japan.
Naoya
N
Minami
Department of Neurology, Hokkaido Medical Center, Sapporo, Japan.
Itaru
I
Amino
Department of Neurology, Hokkaido Medical Center, Sapporo, Japan.
Naoto
N
Fujiki
Department of Neurology, Hokkaido Medical Center, Sapporo, Japan.
Shizuki
S
Doi
Department of Neurology, Hokkaido Medical Center, Sapporo, Japan.
Seiji
S
Kikuchi
D000328:Adult; D002117:Calcitriol / Q000494:pharmacology*; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis; D005260:Female; D006801:Humans; D016753:Interleukin-10 / Q000096:biosynthesis*; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D009103:Multiple Sclerosis / Q000276:immunology / Q000378:metabolism*; D014815:Vitamins / Q000494:pharmacology*
D016207:Cytokines; D014815:Vitamins; D016753:Interleukin-10; D002117:Calcitriol
Cytokine; Disability; IL-10; Multiple sclerosis; Vitamin D