Hypocholesterolemic activity of monascus fermented product in the absence of monacolins with partial purification for functional food applications.
Hypercholesterolemia is one of the most common chronic diseases in human. Along with chemical therapy traditional medication is used as hypocholesterolemic remedy, however, with unfavorable side effects. Recently, Monascus fermented product (MFP) has become a popular hypocholesterolemic natural supplement. In the present study, the hypocholesterolemic activity of Monascus purpureus FTC5391 fermented product ethanolic extract (MFPe) was investigated in hypercholesterolemic rats. Results showed that MFPe not only reduced the serum total cholesterol (TC), LDL-C, TG concentration, and TC/HDL-C ratio but also increased the HDL-C. Further, solid phase extraction (SPE) was carried out to obtain the hypocholesterolemic bioactive fraction. The high polar fraction of SPE increased the HDL-C (42%) and decreased the TC (53.3%), LDL-C (47%), and TG (50.7%) levels as well as TC/HDL-C ratio (69.1%) in serum. The GC-MS results of the active fraction revealed two main compounds, isosorbide and erythritol, which act as coronary vasodilator compounds.
School of Chemical Sciences & Food Technology, Faculty of Science & Technology, MANIS Lab, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia ; Department of Marine Biotechnology, Iranian Fisheries Research Organization, No. 297, West Fatemi Avenue, P.O. Box 14155-6116, Tehran 1411816616, Iran.
Zahra
Z
Ajdari
School of Chemical Sciences & Food Technology, Faculty of Science & Technology, MANIS Lab, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia.
Maaruf
M
Abd Ghani
School of Chemical Sciences & Food Technology, Faculty of Science & Technology, MANIS Lab, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia.
Mohd
M
Khan Ayob
Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
Saadi
0000-0003-3078-8938
S
Bayat
Institute for Environment and Development (LESTARI), Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia.
Mazlin
M
Mokhtar
Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Science, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
Sahar
S
Abbasiliasi
Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
Anahita
A
Khoramnia
Departmet of Microbiology and Pathology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
Heshu Sulaiman
HS
Rahman
Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
Parvaneh
0000-0002-8391-9228
P
Mehrbod
Department of Marine Biotechnology, Iranian Fisheries Research Organization, No. 297, West Fatemi Avenue, P.O. Box 14155-6116, Tehran 1411816616, Iran ; Institute for Environment and Development (LESTARI), Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia.
Daniel
0000-0003-4527-5115
D
Ajdari
Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Science, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
Arbakariya B
AB
Ariff
D000818:Animals; D000924:Anticholesteremic Agents / Q000008:administration & dosage* / Q000302:isolation & purification; D005285:Fermentation / Q000502:physiology; D055951:Functional Food; D006937:Hypercholesterolemia / Q000097:blood* / Q000178:diet therapy*; D008297:Male; D032741:Monascus / Q000302:isolation & purification; D051381:Rats; D017207:Rats, Sprague-Dawley; D016896:Treatment Outcome
D000924:Anticholesteremic Agents