Antioxidant activities of the oligosaccharides from the roots, flowers and leaves of Panax ginseng C.A. Meyer.
The chemical characterization and antioxidant activities of water-soluble ginseng oligosaccharides from roots (WGOS-R), flowers (WGOS-F) and leaves (WGOS-L) of Panax ginseng C.A. Meyer obtained by hot water extraction were investigated. The sugar content of WGOS-R, WGOS-F and WGOS-L were 95.87%, 87.07% and 83.09%, respectively. The ginsenosides and total phenols content decreased in the order of WGOS-L>WGOS-F>WGOS-R. WGOS-R comprised only Glc, WGOS-F and WGOS-L comprised Glucose (Glc) and Rhamnose (Rha) in a molar ratio of 6.0:1.0 and 7.0:1.0, respectively. In vitro antioxidant tests showed that WGOS-R exhibited higher antioxidant activity than WGOS-F and WGOS-L. In vivo antioxidant tests showed that WGOS-R significantly enhanced activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and total antioxidant capability (T-AOC) in the serum and liver and decreased malondialdehyde (MDA) level in the serum and liver.
Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, PR China.
Lili
L
Jiao
Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, PR China.
Bo
B
Li
Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, PR China.
Mingzhu
M
Wang
Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, PR China.
Zhen
Z
Liu
Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, PR China.
Xiaoyu
X
Zhang
Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, PR China; Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China. Electronic address: syliu@ciac.jl.cn.
Shuying
S
Liu
D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000032:analysis; D002374:Catalase / Q000378:metabolism; D004353:Drug Evaluation, Preclinical; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000494:pharmacology; D035264:Flowers / Q000737:chemistry; D016166:Free Radical Scavengers / Q000302:isolation & purification / Q000494:pharmacology; D005979:Glutathione Peroxidase / Q000378:metabolism; D007502:Iron Chelating Agents / Q000302:isolation & purification / Q000494:pharmacology; D008099:Liver / Q000201:enzymology; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D051379:Mice; D008813:Mice, Inbred ICR; D009005:Monosaccharides / Q000032:analysis; D009844:Oligosaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D010084:Oxidation-Reduction; D005894:Panax / Q000737:chemistry*; D010851:Picrates / Q000032:analysis; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D011897:Random Allocation; D013482:Superoxide Dismutase / Q000378:metabolism
D000975:Antioxidants; D001713:Biphenyl Compounds; D004365:Drugs, Chinese Herbal; D016166:Free Radical Scavengers; D007502:Iron Chelating Agents; D009005:Monosaccharides; D009844:Oligosaccharides; D010851:Picrates; D008315:Malondialdehyde; C004931:1,1-diphenyl-2-picrylhydrazyl; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase
Antioxidant activity; Oligosaccharides; Panax ginseng; d-Galctose