Effect of Bridelia ferruginea Benth (Euphorbiaceae) ethyl acetate and acetone fractions on insulin resistance in fructose drinking mice.
BACKGROUND
The root of Bridelia ferruginea is traditionally used as a treatment for type 2 diabetes. The present study was investigated to evaluate the effect of Bridelia ferruginea root bark fractions on some markers of type 2 diabetes on fructose drinking mice.


METHODS
Mice received a solution of fructose 15% during 42 days ad libitum; at the 15th day to the 42nd day, they received distilled water for fructose drinking control group, metformin 50 mg/kg per day or fractions 50 mg/kg per day for treatment groups. The normal control group received only distilled water during the experiment. After 6 weeks of experiment, OGTT, fasting blood glucose, plasma insulin, triglycerides (TG), total cholesterol, AST and ALT levels were measured.


RESULTS
Fructose drinking control group (F) showed significant (p<0.001) increase of glucose tolerance, plasma levels of total cholesterol, triglycerides and insulin index for insulin resistance (Homeostasis Model Assessment ratio HOMA-IR) as compared to normal control mice. In treated groups, there was a significant reduction of glucose intolerance respectively 74% (p<0.001), 25% (p<0.5) and 92% (p<0.001) for ethyl acetate fraction, acetone fraction and metformin at the same dose of 50 mg/kg per day during 4 weeks administration. In ethyl acetate fraction and metformin treated groups, biochemical parameters and insulin index were significantly (p<0.001) lower than that of fructose drinking control group.


CONCLUSIONS
This indicates that Bridelia ferruginea root bark ethyl acetate fraction improved insulin resistance as metformin significantly in type 2 diabetes.
Department of Animal Physiology, Faculty of Sciences, University of Lome, Togo; Department of Pharmacology, University of Bordeaux Segalen, 33076 Bordeaux, France. Electronic address: bbakoma@yahoo.fr.
Batomayena
B
Bakoma
Department of Pharmacology, University of Bordeaux Segalen, 33076 Bordeaux, France.
Bénédicte
B
Berké
Department of Animal Physiology, Faculty of Sciences, University of Lome, Togo.
Kwashie
K
Eklu-Gadegbeku
Department of Animal Physiology, Faculty of Sciences, University of Lome, Togo.
Amegnona
A
Agbonon
Department of Animal Physiology, Faculty of Sciences, University of Lome, Togo.
Kodjo
K
Aklikokou
Department of Animal Physiology, Faculty of Sciences, University of Lome, Togo.
Messanvi
M
Gbeassor
Department of Pharmacology, University of Bordeaux Segalen, 33076 Bordeaux, France.
Nicholas
N
Moore
D000085:Acetates / Q000737:chemistry; D000096:Acetone / Q000737:chemistry; D000818:Animals; D001786:Blood Glucose / Q000187:drug effects; D004326:Drinking; D019665:Euphorbiaceae / Q000737:chemistry; D005632:Fructose / Q000008:administration & dosage; D005951:Glucose Tolerance Test; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D007333:Insulin Resistance; D008813:Mice, Inbred ICR; D024301:Plant Bark / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry; D012997:Solvents / Q000737:chemistry
D000085:Acetates; D001786:Blood Glucose; D007004:Hypoglycemic Agents; D010936:Plant Extracts; D012997:Solvents; D000096:Acetone; D005632:Fructose; C007650:ethyl acetate
Acetone (PubChem CID: 180); Bridelia ferruginea; Chloroform (PubChem CID: 6212); Diabetes; Dichloromethane (PubChem CID: 6344); Ethanol (PubChem CID: 702); Ethyl acetate (PubChem CID: 8095); Fructose; Fructose (PubChem CID: 5984); Glucose (PubChem CID: 79025); Hexane (PubChem CID: 8058); Insulin resistance; Metformin (PubChem CID: 4091)