In vitro exposure of DE-71, a penta-PBDE mixture, on immune endpoints in bottlenose dolphins (Tursiops truncatus) and B6C3F1 mice.
Polybrominated diphenyl ethers (PBDEs) are an emerging contaminant of concern with low level exposures demonstrating toxicity in laboratory animals and wildlife, although immunotoxicity studies have been limited. Bottlenose dolphin peripheral blood leukocytes (PBLs) and mouse splenocytes were exposed to environmentally relevant DE-71 (a penta-PBDE mixture) concentrations (0-50 µg ml(-1) ) in vitro. Natural killer (NK) cell activity and lymphocyte (B and T cell) proliferation were evaluated using the parallelogram approach for risk assessment. This study aimed to substantiate results from field studies with dolphins, assess the sensitivities between the mouse model and dolphins, and to evaluate risk using the parallelogram approach. In mouse cells, NK cell activity increased at in vitro doses 0.05, 0.5 and 25 µg DE-71 ml(-1) , whereas proliferation was not modulated. In dolphin cells, NK cell activity and lymphocyte proliferation was not altered after in vitro exposure. In vitro exposure of dolphin PBLs to DE-71 showed similar results to correlative field studies; NK cell activity in mice was more sensitive to in vitro exposure than dolphins, and the parallelogram approach showed correlation with all three endpoints to predict risk in bottlenose dolphins.
Graduate Program in Marine Biology, College of Charleston, 205 Fort Johnson Road, Charleston, SC, 29412, USA.
Jena R
JR
Wirth
Margie M
MM
Peden-Adams
Natasha D
ND
White
Gregory D
GD
Bossart
Patricia A
PA
Fair
D000818:Animals; D050681:Bottle-Nosed Dolphin / Q000276:immunology*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005260:Female; D055768:Halogenated Diphenyl Ethers / Q000633:toxicity*; D007111:Immunity, Cellular / Q000187:drug effects*; D007694:Killer Cells, Natural / Q000187:drug effects; D007962:Leukocytes / Q000187:drug effects; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects; D008297:Male; D051379:Mice; D013154:Spleen / Q000166:cytology / Q000187:drug effects
D055768:Halogenated Diphenyl Ethers; C086401:pentabromodiphenyl ether
DE-71; Tursiops truncatus; dolphins; immunology; in vitro; lymphocyte proliferation; mouse splenocytes; natural killer (NK) cell activity; polybrominated diphenyl ethers (PBDE)