Completely green synthesis of dextrose reduced silver nanoparticles, its antimicrobial and sensing properties.
We herein report the green synthesis of highly monodispersed, water soluble, stable and smaller sized dextrose reduced gelatin capped-silver nanoparticles (Ag-NPs) via an eco-friendly, completely green method. The synthesis involves the use of silver nitrate, gelatin, dextrose and water as the silver precursor, stabilizing agent, reducing agent and solvent respectively. By varying the reaction time, the temporal evolution of the growth, optical, antimicrobial and sensing properties of the as-synthesised Ag-NPs were investigated. The nanoparticles were characterized using UV-vis absorption spectroscopy, Fourier transform infra-red spectroscopy (FT-IR), X-ray diffraction (XRD), transmission electron microscopy (TEM) and high resolution transmission electron microscopy (HR-TEM). The absorption maxima of the as-synthesized materials at different reaction time showed characteristic silver surface plasmon resonance (SPR) peak. The as-synthesised Ag-NPs show better antibacterial efficacy than the antibiotics; ciproflaxin and imipenem against Pseudomonas aeruginosa with minimum inhibition concentration (MIC) of 6 μg/mL, and better efficacy than imipenem against Escherichia coli with MIC of 10 μg/mL. The minimum bactericidal concentration (MBC) of the as-synthesised Ag-NPs is 12.5 μg/mL. The sensitivity of the dextrose reduced gelatin-capped Ag-NPs towards hydrogen peroxide indicated that the sensor has a very good sensitivity and a linear response over wide concentration range of 10(-1)-10(-6)M H2O2.
Centre for Nanoscience and Nanotechnology, Mahatma Gandhi University, Kottayam, Kerala 686560, India.
Sneha
S
Mohan
Department of Chemistry, Cape-peninsula University of Technology, P.O.Box 652, Cape town, 8000, South Africa. Electronic address: oluwafemi.oluwatobi@gmail.com.
Oluwatobi S
OS
Oluwafemi
Amal Jyothi College of Engineering, Kanjirappally, Kottayam, Kerala 686560, India.
Soney C
SC
George
Antibacterials and Microbial Technology Lab, Pushpagiri Research Centre, Pushpagiri Institute of Medical Sciences, Tiruvalla, Kerala 689 101, India.
V P
VP
Jayachandran
Department of Agriculture, Cape-peninsula University of Technology, Wellington, 7655, South Africa.
Francis B
FB
Lewu
Department of Chemistry, Walter Sisulu University, Private Bag X1, Mthatha, 5117, Eastern Cape, South Africa.
Sandile P
SP
Songca
Centre for Nanoscience and Nanotechnology, Mahatma Gandhi University, Kottayam, Kerala 686560, India; School of Pure and Applied Physics, Mahatma Gandhi University, Kottayam, Kerala 686560, India.
Nandakumar
N
Kalarikkal
Centre for Nanoscience and Nanotechnology, Mahatma Gandhi University, Kottayam, Kerala 686560, India; School of Chemical Sciences, Mahatma Gandhi University, Kottayam, Kerala 686560, India. Electronic address: sabupolymer@yahoo.com.
Sabu
S
Thomas
D000900:Anti-Bacterial Agents / Q000138:chemical synthesis* / Q000494:pharmacology; D002939:Ciprofloxacin / Q000494:pharmacology; D004353:Drug Evaluation, Preclinical; D004926:Escherichia coli / Q000187:drug effects*; D005780:Gelatin; D005947:Glucose / Q000737:chemistry*; D055772:Green Chemistry Technology; D006861:Hydrogen Peroxide / Q000032:analysis; D015378:Imipenem / Q000494:pharmacology; D053768:Metal Nanoparticles / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D008854:Microscopy, Electron; D010084:Oxidation-Reduction; D011550:Pseudomonas aeruginosa / Q000187:drug effects*; D012680:Sensitivity and Specificity; D012834:Silver / Q000737:chemistry*; D012835:Silver Nitrate / Q000737:chemistry; D012995:Solubility; D013053:Spectrophotometry; D017550:Spectroscopy, Fourier Transform Infrared; D020349:Surface Plasmon Resonance
D000900:Anti-Bacterial Agents; D012834:Silver; D002939:Ciprofloxacin; D015378:Imipenem; D005780:Gelatin; D012835:Silver Nitrate; D006861:Hydrogen Peroxide; D005947:Glucose
Antibacterial; Dextrose; Gelatin; Green synthesis; Sensor; Silver nanoparticles