Characterisation of hyaluronic acid and chondroitin/dermatan sulfate from the lumpsucker fish, C. lumpus.
The lumpsucker, Cyclopterus lumpus, a cottoid teleost fish found in the cold waters of the North Atlantic, and North Pacific, was identified as a possible source of GAGs. The GAGs present in the C. lumpus dorsal hump and body wall tissue were isolated and purified. Two fractions were analysed by NMR and their GAG structures determined as hyaluronic acid and CS/DS chains. The latter fraction contained GlcA (65% of the total uronic acids) and IdoA (the remaining 35%). All uronic acid residues were unsulfated, whilst 86% of the GalNAc was 4-sulfated and 14% was 6-sulfated. The presence of GlcA-GalNAc4S, IdoA-GalNAc4S and GlcA-GalNAc6S disaccharide fragments was confirmed. The isolated GAGs obtained from each tissue were biochemically characterised. The lumpsucker offers a high yield source of GAGs, which compares favourably with other sources such as shark cartilage.
EaStCHEM School of Chemistry, Joseph Black Building, The King's Buildings, Edinburgh EH9 3JJ, UK. Electronic address: cpanagos@staffmail.ed.ac.uk.
Charalampos G
CG
Panagos
GlycoMar Ltd, European Centre for Marine Biotechnology, Oban, Scotland PA37 1QA, UK. Electronic address: derek@glycomar.com.
Derek
D
Thomson
GlycoMar Ltd, European Centre for Marine Biotechnology, Oban, Scotland PA37 1QA, UK. Electronic address: claire@glycomar.com.
Claire
C
Moss
GlycoMar Ltd, European Centre for Marine Biotechnology, Oban, Scotland PA37 1QA, UK. Electronic address: charlie@glycomar.com.
Charles D
CD
Bavington
Biopol, Einbúastíg 2, 545 Skagaströnd, Iceland. Electronic address: halldor@biopol.is.
Halldór G
HG
Olafsson
EaStCHEM School of Chemistry, Joseph Black Building, The King's Buildings, Edinburgh EH9 3JJ, UK. Electronic address: dusan.uhrin@ed.ac.uk.
Dušan
D
Uhrín
D000818:Animals; D002809:Chondroitin Sulfates / Q000737:chemistry* / Q000302:isolation & purification; D003871:Dermatan Sulfate / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification; D005399:Fishes / Q000378:metabolism*; D006820:Hyaluronic Acid / Q000737:chemistry* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D010084:Oxidation-Reduction; D011108:Polymers / Q000737:chemistry*
D011108:Polymers; C069524:dermatan sulfate chondroitin sulfate; D003871:Dermatan Sulfate; D006820:Hyaluronic Acid; D002809:Chondroitin Sulfates
Chondroitin sulfate; Cyclopterus lumpus; Dermatan sulfate; Glycosaminoglycan; Hyaluronic acid; NMR