A systematic proteomic analysis of NaCl-stressed germinating maize seeds.
Salt (NaCl) is a common physiological stressor of plants. To better understand how germinating seeds respond to salt stress, we examined the changes that occurred in the proteome of maize seeds during NaCl-treated germination. Phenotypically, salt concentrations less than 0.2 M appear to delay germination, while higher concentrations disrupt development completely, leading to seed death. The identities of 96 proteins with expression levels altered by NaCl-incubation were established using 2-DE-MALDI-TOF-MS and 2-DE-MALDI-TOF-MS/MS. Of these 96 proteins, 79 were altered greater than twofold when incubated with a 0.2 M salt solution, while 51 were altered when incubated with a 0.1 M salt solution. According to their functional annotations in the Swiss-Prot protein-sequence databases, these proteins are mainly involved in seed storage, energy metabolism, stress response, and protein metabolism. Notably, the expression of proteins that respond to abscisic acid signals increased in response to salt stress. The results of this study provide important clues as to how NaCl stresses the physiology of germinating maize seeds.
Department of Life Science, Harbin University, Harbin, 150086, People's Republic of China.
Ling-Bo
LB
Meng
Yi-Bo
YB
Chen
Tian-Cong
TC
Lu
Yue-Feng
YF
Wang
Chun-Rong
CR
Qian
Yang
Y
Yu
Xuan-Liang
XL
Ge
Xiao-Hui
XH
Li
Bai-Chen
BC
Wang
D004734:Energy Metabolism; D018525:Germination; D010641:Phenotype; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017510:Protein Folding; D055550:Protein Stability; D020543:Proteome; D040901:Proteomics / Q000379:methods; D055049:Salt Tolerance / Q000235:genetics; D012639:Seeds / Q000187:drug effects / Q000378:metabolism*; D012965:Sodium Chloride / Q000378:metabolism* / Q000494:pharmacology; D013312:Stress, Physiological; D003313:Zea mays / Q000187:drug effects / Q000378:metabolism*
D010940:Plant Proteins; D020543:Proteome; D012965:Sodium Chloride