Oil bodies as a potential microencapsulation carrier for astaxanthin stabilisation and safe delivery.
Astaxanthin (AST) is a valued molecule because of its high antioxidant properties. However, AST is extremely sensitive to oxidation, causing the loss of its bioactive properties. The purposes of this study were to define conditions for microencapsulating AST in oil bodies (OB) from Brassica napus to enhance its oxidative stability, and to test the bioactivity of the microencapsulated AST (AST-M) in cells. Conditions for maximising microencapsulation efficiency (ME) were determined using the Response Surface Methodology, obtaining a high ME (>99%). OB loaded with AST showed a strong electrostatic repulsion in a wide range of pH and ionic strengths. It was found that AST-M exposed to air and light was more stable than free AST. In addition, the protective effect of AST against intracellular ROS production was positively influenced by microencapsulation in OB. These results suggest that OB offer a novel option for stabilising and delivering AST.
Agriaquaculture Nutritional Genomic Center, CGNA, Technology and Processes Unit , Temuco , Chile .
Francisca
F
Acevedo
Mónica
M
Rubilar
Ignacio
I
Jofré
Mario
M
Villarroel
Patricia
P
Navarrete
Magdalena
M
Esparza
Fernando
F
Romero
Elías Alberto
EA
Vilches
Valentina
V
Acevedo
Carolina
C
Shene
D000975:Antioxidants / Q000008:administration & dosage* / Q000494:pharmacology; D029688:Brassica napus / Q000737:chemistry*; D002460:Cell Line; D004337:Drug Carriers / Q000737:chemistry*; D004339:Drug Compounding; D006801:Humans; D066292:Lipid Droplets / Q000737:chemistry*; D017382:Reactive Oxygen Species / Q000378:metabolism; D024341:Xanthophylls / Q000008:administration & dosage / Q000494:pharmacology
D000975:Antioxidants; D004337:Drug Carriers; D017382:Reactive Oxygen Species; D024341:Xanthophylls; C005948:astaxanthine
Antioxidant effect; astaxanthin; cell viability; microencapsulation; oil bodies; oleosomes