Identification of COL3A1 and RAB2A as novel translocation partner genes of PLAG1 in lipoblastoma.
Lipoblastoma is a rapidly growing, benign neoplasm in children. Surgical excision is usually curative, with a recurrence rate of about 20%. Because the histology of lipoblastoma is heterogeneous and overlaps with other lipomatous tumors, some lipoblastoma cases have been difficult to diagnose. The detection of PLAG1 gene rearrangement is useful for the diagnosis of lipoblastoma. Three fusion partner genes are known in relation to PLAG1 in lipoblastoma HAS2 at 8q24.1, COL1A2 at 7q22, and RAD51L1 at 14q24. Herein, we describe another two novel fusion genes in lipoblastoma tumor specimens. We checked six tumors for the presence of two known fusion genes, HAS2-PLAG1 and COL1A2-PLAG1. Only HAS2-PLAG1 was found in one of the cases. Next, we attempted to identify potential PLAG1 fusion partners using 5'RACE. Sequence analysis revealed two novel fusion genes, COL3A1-PLAG1 in three cases and RAB2A-PLAG1 in one case, respectively. As a result of the translocations, the constitutively active promoter of the partner gene drives the ectopic expression of PLAG1. We also evaluated whether a high level of PLAG1 expression can be used to help differentiate lipomatous tumors. PLAG1 expression was evaluated by real-time PCR in five lipoblastoma tumor specimens. The expressions were 70-150 times higher in lipoblastomas than in human adipocytes. However, PLAG1 expression was low in one case of lipoma. These results demonstrate that PLAG1 overexpression is a potential marker of lipoblastoma. Our findings, in agreement with previous studies, show that lipoblastoma is a group of lipomatous tumors with PLAG1 rearrangement and overexpression. © 2014 Wiley Periodicals, Inc.
Department of Pediatrics, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto, Japan.
Hideki
H
Yoshida
Mitsuru
M
Miyachi
Kazutaka
K
Ouchi
Yasumichi
Y
Kuwahara
Kunihiko
K
Tsuchiya
Tomoko
T
Iehara
Eiichi
E
Konishi
Akio
A
Yanagisawa
Hajime
H
Hosoi
D014408:Biomarkers, Tumor / Q000235:genetics; D002675:Child, Preschool; D024061:Collagen Type III / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D005260:Female; D050939:Gene Fusion; D020662:Guanine Nucleotide Exchange Factors; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D062689:Lipoblastoma / Q000175:diagnosis / Q000235:genetics*; D008297:Male; D011379:Prognosis; D014178:Translocation, Genetic; D020691:rab GTP-Binding Proteins / Q000235:genetics*
D014408:Biomarkers, Tumor; C439971:COL3A1 protein, human; D024061:Collagen Type III; D004268:DNA-Binding Proteins; D020662:Guanine Nucleotide Exchange Factors; C104382:PLAG1 protein, human; C102739:RGL2 protein, human; D020691:rab GTP-Binding Proteins