Coordination complexes as molecular glue for immobilization of antibodies on cyclic olefin copolymer surfaces.
A novel metal-based chelating method has been used to provide an order of magnitude increase in immunoassay performance on cyclic olefin copolymer (COC) plastics compared with passive binding. COCs are hydrophobic, and without surface modification they are often unsuitable for applications where protein adhesion is desired. When interacting with the bare plastic, the majority of the bound proteins will be denatured and become nonfunctional. Many of the surface modification techniques reported to date require costly equipment setup or the use of harsh reaction conditions. Here, we have successfully demonstrated the use of a simple and quick metal chelation method to increase the sensitivity, activity, and efficiency of protein binding to COC surfaces. A detailed analysis of the COC surfaces after activation with the metal complexes is presented, and the immunoassay performance was studied using three different antibody pairs.
Anteo Diagnostics, Eight Mile Plains, 4113 QLD, Australia; Australian Institute for Bioengineering and Nanotechnology, University of Queensland, Brisbane, 4072 QLD, Australia.
Huey Wen
HW
Ooi
Anteo Diagnostics, Eight Mile Plains, 4113 QLD, Australia.
Shaun J
SJ
Cooper
Anteo Diagnostics, Eight Mile Plains, 4113 QLD, Australia.
Chang-Yi
CY
Huang
Anteo Diagnostics, Eight Mile Plains, 4113 QLD, Australia.
Dean
D
Jennins
Anteo Diagnostics, Eight Mile Plains, 4113 QLD, Australia.
Emma
E
Chung
Anteo Diagnostics, Eight Mile Plains, 4113 QLD, Australia.
N Joe
NJ
Maeji
Australian Institute for Bioengineering and Nanotechnology, University of Queensland, Brisbane, 4072 QLD, Australia; Centre for Advanced Imaging, University of Queensland, Brisbane, 4072 QLD, Australia. Electronic address: a.whittaker@uq.edu.au.
Andrew K
AK
Whittaker
D055910:Antibodies, Immobilized / Q000737:chemistry*; D056831:Coordination Complexes / Q000737:chemistry*; D003516:Cycloparaffins / Q000737:chemistry*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D007118:Immunoassay; D011108:Polymers / Q000737:chemistry*; D013499:Surface Properties
D055910:Antibodies, Immobilized; D056831:Coordination Complexes; D003516:Cycloparaffins; D011108:Polymers
Cyclic olefin copolymers; Enzyme-linked immunosorbent assay; Immunoassay; Metal complexes; Surface functionalization