Variability in DNA damage of chub (Squalius cephalus L.) blood, gill and liver cells during the annual cycle.
In this work the genotoxic potential of water in three localities in Serbia, which differ by the nature and degree of pollution, was determined in tissues of European chub (Squalius cephalus L.) on monthly basis over the 2011/2012 year season using the alkaline comet assay. Specimen samples of chub were taken from Special Nature Reserve "Uvac", as control site, and Pestan and Beljanica Rivers, as polluted sites at Kolubara basin, surrounded with coal mines. Three tissues, blood, gills and liver were used for assessing the level of DNA damage. Analysis was done by software (Comet Assay IV). The control site at Reserve "Uvac" showed the lowest DNA damage values for all three tissues compared to Pestan and Beljanica. Blood has the lowest level of DNA damage in comparison with liver and gills. Decreased damage for all three tissues was observed at summer, while during the spring and autumn damage increased.
Department of Natural Resources and Environmental Sciences, Institute for Multidisciplinary Research, Kneza Višeslava 1, University of Belgrade, Belgrade, Serbia.
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K
Sunjog
Center for Genotoxicology and Ecogenotoxicology, Chair of Microbiology, Faculty of Biology, Studenski Trg 16, University of Belgrade, Belgrade, Serbia.
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S
Kolarević
Institute for Biological Research "Siniša Stanković", Despota Stefana 142, University of Belgrade, Belgrade, Serbia.
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M
Kračun-Kolarević
Department of Natural Resources and Environmental Sciences, Institute for Multidisciplinary Research, Kneza Višeslava 1, University of Belgrade, Belgrade, Serbia.
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Z
Gačić
Department of Natural Resources and Environmental Sciences, Institute for Multidisciplinary Research, Kneza Višeslava 1, University of Belgrade, Belgrade, Serbia.
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S
Skorić
Institute for Biological Research "Siniša Stanković", Despota Stefana 142, University of Belgrade, Belgrade, Serbia.
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V
Ðikanović
Department of Natural Resources and Environmental Sciences, Institute for Multidisciplinary Research, Kneza Višeslava 1, University of Belgrade, Belgrade, Serbia. Electronic address: lenhardt@ibiss.bg.ac.rs.
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M
Lenhardt
Center for Genotoxicology and Ecogenotoxicology, Chair of Microbiology, Faculty of Biology, Studenski Trg 16, University of Belgrade, Belgrade, Serbia.
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B
Vuković-Gačić
D000818:Animals; D020552:Comet Assay; D003530:Cyprinidae; D004249:DNA Damage; D004784:Environmental Monitoring; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism; D005880:Gills / Q000166:cytology / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000378:metabolism; D045483:Rivers; D055771:Serbia; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D014874:Water Pollutants, Chemical
Comet assay; European chub; Genotoxicity; Water pollution