Oxidative stress and DNA damage responses to phenanthrene exposure in the estuarine guppy Poecilia vivipara.
Despite ubiquitous phenanthrene contamination in aquatic coastal areas, little is known regarding its potential effects on estuarine fishes. The present work evaluated the response of a large suite of oxidative stress- and DNA damage-related biomarkers to phenanthrene exposure (10, 20 and 200 μg L(-1), 96 h) using DMSO as the solvent in estuarine guppy Poecilia vivipara (salinity 24 psu). Phenanthrene affected oxidative stress-related parameters, and decreased antioxidant defenses and reactive oxygen species in the gills and muscle overall. Lipid peroxidation occurred in muscle at 200 μg L(-1) phenanthrene. Genotoxicity was increased at 20 μg L(-1), while 200 μg L(-1) caused a relative decrease in erythrocyte release into the bloodstream. These findings indicated that phenanthrene is genotoxic and can induce oxidative stress, depending on tissue and phenanthrene concentration analyzed. Thus, some of the biomarkers analyzed in the present study are sufficiently sensitive to monitor the exposure of the guppy P. vivipara to phenanthrene in salt water. However, further studies are required for a better interpretation of the dose-response patterns observed.
Universidade Federal do Rio Grande, Programa de Pós-Graduação em Oceanografia Biológica, Av. Itália km 8, 96201-900 Rio Grande, Rio Grande do Sul, Brazil. Electronic address: machadoaas@gmail.com.
Anderson Abel de Souza
AA
Machado
Universidade Federal do Rio Grande, Instituto de Ciências Biológicas, Av. Itália km 8, 96201-900 Rio Grande, Rio Grande do Sul, Brazil. Electronic address: hoffmlm@yahoo.com.br.
Mariana Leivas Müller
ML
Hoff
Universidade Federal do Rio Grande, Programa de Pós-Graduação em Ciências Fisiológicas - Fisiologia Animal Comparada, Av. Itália km 8, 96201-900 Rio Grande, Rio Grande do Sul, Brazil. Electronic address: robertadaniele@yahoo.com.br.
Roberta Daniele
RD
Klein
Universidade Federal do Rio Grande, Instituto de Ciências Biológicas, Av. Itália km 8, 96201-900 Rio Grande, Rio Grande do Sul, Brazil. Electronic address: gilsoncord@yahoo.com.br.
Gilson Junior
GJ
Cordeiro
Universidade Federal do Rio Grande, Instituto de Oceanografia, Av. Itália km 8, 96201-900 Rio Grande, Rio Grande do Sul, Brazil. Electronic address: nineavila@gmail.com.
Jannine Marquez
JM
Lencina Avila
Universidade Federal do Rio Grande, Instituto de Oceanografia, Av. Itália km 8, 96201-900 Rio Grande, Rio Grande do Sul, Brazil. Electronic address: patcosta0@gmail.com.
Patrícia Gomes
PG
Costa
Universidade Federal do Rio Grande, Instituto de Ciências Biológicas, Av. Itália km 8, 96201-900 Rio Grande, Rio Grande do Sul, Brazil. Electronic address: adaltobianchini@furg.br.
Adalto
A
Bianchini
D000818:Animals; D015415:Biomarkers / Q000032:analysis; D004249:DNA Damage / Q000187:drug effects*; D005880:Gills / Q000187:drug effects; D018482:Muscle, Skeletal / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D010616:Phenanthrenes / Q000633:toxicity*; D011036:Poecilia / Q000502:physiology*; D017382:Reactive Oxygen Species / Q000032:analysis; D012623:Seawater; D014874:Water Pollutants, Chemical / Q000633:toxicity
D015415:Biomarkers; D010616:Phenanthrenes; D017382:Reactive Oxygen Species; D014874:Water Pollutants, Chemical; C031181:phenanthrene
Biomarkers; DNA damage; Oxidative stress; Phenanthrene; Poecilia vivipara; Salt water