The multifaceted effects of polysaccharides isolated from Dendrobium huoshanense on immune functions with the induction of interleukin-1 receptor antagonist (IL-1ra) in monocytes.
Dendrobium huoshanense is a valuable and versatile Chinese herbal medicine with the anecdotal claims of cancer prevention and anti-inflammation. However, its immunological activities are limited to in vitro studies on a few cytokines and immune cell functions. First, we investigated the effects of polysaccharides isolated from DH (DH-PS) on inducing a panel of cytokines/chemokines in mice in vivo and human in vitro. We found that DH polysaccharides (DH-PS) induced TH1, TH2, inflammatory cytokines and chemokines in mouse in vivo and human cells in vitro. Secondly, we demonstrated that DH-PS expanded mouse splenocytes in vivo including CD4(+) T cells, CD8(+) T cells, B cells, NK cells, NKT cells, monocytes/macrophages, granulocytes and regulatory T cells. Notably, DH-PS induced an anti-inflammatory molecule, IL-1ra, in mouse and human immune cells, especially monocytes. The serum level of IL-1ra elicited by the injection of DH-PS was over 10 folds of IL-1β, suggesting that DH-PS-induced anti-inflammatory activities might over-ride the inflammatory ones mediated by IL-1β. The signaling pathways of DH-PS-induced IL-1ra production was shown to involve ERK/ELK, p38 MAPK, PI3K and NFκB. Finally, we observed that IL-1ra level induced by DH-PS was significantly higher than that by F3, a polysaccharide extract isolated from another popular Chinese herbal medicine, Ganoderma lucidum. These results indicated that DH-PS might have potential applications for ameliorating IL-1-induced pathogenic conditions.
Institute of Biochemical Sciences, National Taiwan University, Taipei, Taiwan; Genomics Research Center, Academia Sinica, Taipei, Taiwan.
Juway
J
Lin
Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan.
Ya-Jen
YJ
Chang
Genomics Research Center, Academia Sinica, Taipei, Taiwan.
Wen-Bin
WB
Yang
Genomics Research Center, Academia Sinica, Taipei, Taiwan; Department of Pediatrics/Hematology-Oncology, University of California San Diego Medical Center, San Diego, California, United States of America; Center of Stem Cell & Translational Cancer Research, Chang Gung Memorial Hospital, Taoyuan, Taiwan.
Alice L
AL
Yu
Genomics Research Center, Academia Sinica, Taipei, Taiwan; The Scripps Research Institute, La Jolla, California, United States of America.
Chi-Huey
CH
Wong
D000818:Animals; D018925:Chemokines / Q000096:biosynthesis; D016207:Cytokines / Q000096:biosynthesis; D031667:Dendrobium / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D005260:Female; D006801:Humans; D055633:Immune System Phenomena / Q000187:drug effects / Q000502:physiology; D007155:Immunologic Factors / Q000276:immunology / Q000494:pharmacology; D053590:Interleukin 1 Receptor Antagonist Protein / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D051379:Mice; D009000:Monocytes / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism*; D009504:Neutrophils; D053147:Plant Exudates / Q000494:pharmacology; D011134:Polysaccharides / Q000276:immunology / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects
D018925:Chemokines; D016207:Cytokines; D004365:Drugs, Chinese Herbal; D007155:Immunologic Factors; D053590:Interleukin 1 Receptor Antagonist Protein; D053147:Plant Exudates; D011134:Polysaccharides