Nanoscale probing the kinetics of oriented bacterial cell growth using atomic force microscopy.
Probing oriented bacterial cell growth on the nanoscale: A novel open-top micro-channel is developed to facilitate the AFM imaging of physically trapped but freely growing bacteria. The growth curves of individual Escherichia coli cells with nanometer resolution and their kinetic nano-mechanical properties are quantitatively measured.
Institut National de la Santé et de la Recherche Medicale, U1001; Faculty of Medicine, Paris Descartes University, 75014, Paris, France.
Peipei
P
Chen
Luping
L
Xu
Jing
J
Liu
Felix J H
FJ
Hol
Juan E
JE
Keymer
François
F
Taddei
Dong
D
Han
Ariel B
AB
Lindner
D049108:Cell Enlargement; D049109:Cell Proliferation / Q000502:physiology*; D002469:Cell Separation / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D004926:Escherichia coli / Q000254:growth & development* / Q000648:ultrastructure*; D007700:Kinetics; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D018625:Microscopy, Atomic Force / Q000295:instrumentation*
atomic force microscopy; bacteria; biochips; kinetics; oriented growth