Effects of Cydonia oblonga Miller extracts on blood hemostasis, coagulation and fibrinolysis in mice, and experimental thrombosis in rats.
BACKGROUND
Cydonia oblonga Miller (COM) is traditionally used in Uyghur medicine for the prevention of cardiovascular disease. The present study is designed to explore the effects of COM extracts on models and markers of thrombosis and related biomarkers.


METHODS
20, 40, 80 mg/kg/day COM aqueous extracts and 5mg/kg/day aspirin, orally for 14 days were compared to untreated controls in mice on bleeding and clotting times, using the tail cutting and glass slide methods and for death rates in collagen-epinephrine pulmonary thrombosis, thrombolysis in vitro and euglobulin lysis time (ELT). In rats, common carotid artery FeCl3-induced thrombus and inferior vena cava thrombosis occlusion time, plasma concentrations of thromboxane B2 (TXB2) and 6-keto-prostaglandine F1α (6-keto-PGF1α) were measured.


CONCLUSIONS
Compared to controls, COM extracts dose-dependently prolonged bleeding by 2.17, 2.78 and 3.63 times, vs. aspirin 2.58, and the clotting time by 1.44, 2.47 and 2.48 times, vs. aspirin 1.91. COM reduced pulmonary embolus mortality by 27, 40 and 53%, vs. 47% for aspirin. COM dose-dependently increased thrombolysis by 45, 55 and 63%, vs. 56% for aspirin, and shortened ELT to 71, 61 and 43%, vs. 43% for aspirin. In rats, venous occlusion time was prolonged. Arterial and venous thrombus weights were dose-dependently reduced in COM groups. TXB2 decreased and 6-keto-PGF1α increased with COM and aspirin, with an association between 6-keto-PGF1α/TXB2 and arterial or venous thrombus weight for all products, and for occlusion time with COM but not for aspirin.


CONCLUSIONS
We confirm the experimental effects of COM on hemostasis and thrombosis. Further exploration of putative clinical effects appear justified.
Department of Pharmacology, Xinjiang Medical University, 830011 Urumqi, Xinjiang, People׳s Republic of China.
Wenting
W
Zhou
Department of Pharmacology, Xinjiang Medical University, 830011 Urumqi, Xinjiang, People׳s Republic of China.
Adil
A
Abdurahman
Department of Pharmacology, Xinjiang Medical University, 830011 Urumqi, Xinjiang, People׳s Republic of China; Department of Pharmacology, Université Bordeaux Segalen, F-33076 Bordeaux, France. Electronic address: Anwar.umar@126.com.
Anwar
A
Umar
Department of Pharmacology, Xinjiang Medical University, 830011 Urumqi, Xinjiang, People׳s Republic of China.
Guldiyar
G
Iskander
Department of Pharmacology, Xinjiang Medical University, 830011 Urumqi, Xinjiang, People׳s Republic of China.
Elzira
E
Abdusalam
Department of Pharmacology, Université Bordeaux Segalen, F-33076 Bordeaux, France.
Benedicte
B
Berké
Department of Pharmacology, Université Bordeaux Segalen, F-33076 Bordeaux, France.
Bernard
B
Bégaud
Department of Pharmacology, Xinjiang Medical University, 830011 Urumqi, Xinjiang, People׳s Republic of China; Department of Pharmacology, Université Bordeaux Segalen, F-33076 Bordeaux, France. Electronic address: nicholas.moore@pharmaco.u-bordeaux2.fr.
Nicholas
N
Moore
D015121:6-Ketoprostaglandin F1 alpha / Q000097:blood; D000818:Animals; D001777:Blood Coagulation / Q000187:drug effects; D002317:Cardiovascular Agents / Q000494:pharmacology / Q000627:therapeutic use*; D002341:Carotid Artery Thrombosis / Q000139:chemically induced / Q000188:drug therapy*; D002712:Chlorides; D003094:Collagen; D004837:Epinephrine; D005290:Ferric Compounds; D005342:Fibrinolysis / Q000187:drug effects; D006487:Hemostasis / Q000187:drug effects; D008297:Male; D008813:Mice, Inbred ICR; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D018515:Plant Leaves; D011655:Pulmonary Embolism / Q000139:chemically induced / Q000188:drug therapy*; D017208:Rats, Wistar; D027824:Rosaceae; D013929:Thromboxane B2 / Q000097:blood; D014682:Vena Cava, Inferior; D020246:Venous Thrombosis / Q000139:chemically induced / Q000188:drug therapy*
D002317:Cardiovascular Agents; D002712:Chlorides; D005290:Ferric Compounds; D010936:Plant Extracts; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha; D003094:Collagen; C024555:ferric chloride; D004837:Epinephrine
6-keto-PGF 1α; Arterial thrombosis; Cydonia oblonga Miller; Thrombolysis; Thromboxane; Venous occlusion