Inactivation of the LOX-1 pathway promotes the Golgi apparatus during cell differentiation of mural granulosa cells.
In female mammals, granulosa cells of the ovarian follicle differentiate into the corpus luteum after ovulation of the pregnable oocyte into the fallopian tube. During these differentiation processes several morphological alterations have to occur and the molecular basis is not fully understood. As an endpoint estradiol production from granulosa cells has to switch off in favor for progesterone production from the proceeding corpus luteum to sustain the developing embryo. Previously, we demonstrated that the multiligand receptor LOX-1 plays a critical role in steroid hormone synthesis of granulosa cells via intracellular calcium release from endoplasmic (ER)-dependent and ER-independent calcium pools. In the present study, we show that inhibition of LOX-1 leads to a rearrangement of ceramide from the basal membrane toward the Golgi apparatus. This activity is accomplished by a calcium-dependent phosphorylation of aromatase, the key step in estradiol production. Phosphorylated aromatase increased estradiol production in a dose-dependent manner. Our data indicate that the ceramide cascade is essential for proper granulosa cell function and ceramide redistribution serves as a first step in order to proceed with the prosperous differentiation into a corpus luteum.
Institute of Reproductive Biology, Leibniz Institute for Farm Animal Biology (FBN), Dummerstorf, Germany.
J M
JM
Weitzel
A
A
Vernunft
B
B
Krüger
C
C
Plinski
T
T
Viergutz
D000818:Animals; D002417:Cattle; D002454:Cell Differentiation / Q000235:genetics*; D002518:Ceramides / Q000378:metabolism; D003338:Corpus Luteum / Q000166:cytology / Q000378:metabolism; D047108:Embryonic Development / Q000235:genetics*; D005260:Female; D005640:Follicle Stimulating Hormone / Q000378:metabolism; D006056:Golgi Apparatus / Q000235:genetics; D006107:Granulosa Cells / Q000166:cytology / Q000378:metabolism; D006801:Humans; D009865:Oocytes / Q000166:cytology* / Q000254:growth & development; D006080:Ovarian Follicle / Q000166:cytology / Q000378:metabolism; D051127:Scavenger Receptors, Class E / Q000037:antagonists & inhibitors / Q000235:genetics*; D015398:Signal Transduction
D002518:Ceramides; C495137:OLR1 protein, human; D051127:Scavenger Receptors, Class E; D005640:Follicle Stimulating Hormone