Vitamin E, γ-tocopherol, diminishes ex vivo basophil response to dust mite allergen.
Epidemiologic studies suggest that dietary vitamin E is a candidate intervention for atopic disease. We used in vitro and ex vivo exposures to test the hypothesis that the most common dietary isoform of vitamin E, γ-tocopherol (γT), could suppress FcεRI-mediated basophil activation. Rat basophilic leukemia (RBL)-SX38 cells that express human FcεRI were treated with or without γT, followed by stimulation with α-IgE. In the ex vivo study, 20 Der f 1-allergic volunteers consumed a γT-enriched supplement for 7 days. Their basophils were challenged ex vivo with α-IgE and graded doses of Der f 1 before and after the supplementation period. γt treatment of RBL-SX38 cells significantly reduced basophil degranulation and de novo TH2 cytokine production. Daily consumption of a γT-rich supplement by dust mite-allergic volunteers reduced basophil activation after ex vivo dust mite challenge. Vitamin E supplements rich in γT may be useful adjuncts in decreasing atopic disease.
K
K
Mills
J
J
Lay
W
W
Wu
C
C
Robinette
M J
MJ
Kesic
S C
SC
Dreskin
D B
DB
Peden
M
M
Hernandez
D039741:Antigens, Dermatophagoides / Q000276:immunology*; D001491:Basophils / Q000187:drug effects* / Q000276:immunology*; D015550:Cell Degranulation / Q000187:drug effects / Q000276:immunology; D002460:Cell Line; D016207:Cytokines / Q000096:biosynthesis; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology; D017998:Leukotriene D4 / Q000378:metabolism; D014810:Vitamin E / Q000494:pharmacology*; D024504:gamma-Tocopherol / Q000494:pharmacology*
D039741:Antigens, Dermatophagoides; D016207:Cytokines; D014810:Vitamin E; D007073:Immunoglobulin E; D017998:Leukotriene D4; D024504:gamma-Tocopherol