Physiological indices of stress in wild and captive garter snakes: correlations, repeatability, and ecological variation.
Glucocorticoids and leukocyte ratios have become the most widespread variables employed to test hypotheses regarding physiological stress in wild and captive vertebrates. Little is known, however, regarding how these two indices of stress covary in response to stressors, their repeatability within individuals, and differences in response time upon capture. Furthermore, few studies compare stress indices between captive and wild populations, to assess potential alteration of stress physiology in captivity. To address these issues, we examined corticosterone (CORT) and heterophil to lymphocyte (H:L) ratios in two ecotypes of the garter snake Thamnophis elegans. We found that CORT and H:L ratios were not correlated within individuals, and both variables showed little or no repeatability over a period of months. CORT levels, but not H:L ratios, were higher for individuals sampled after 10min from the time of capture. However, both variables showed similar patterns of ecotypic variation, and both increased over time in gravid females maintained in captivity for four months. We suggest that CORT and H:L ratios are both useful, but disparate indices of stress in this species, and may show complex relationships to each other and to ecological and anthropogenic variables.
Department of Biology, Westmont College, Santa Barbara, CA 93108, USA. Electronic address: sparkman@westmont.edu.
Amanda M
AM
Sparkman
Department of Ecology, Evolution, and Organismal Biology, Iowa State University, Ames, IA 50011, USA.
Anne M
AM
Bronikowski
Department of Ecology, Evolution, and Organismal Biology, Iowa State University, Ames, IA 50011, USA.
Shelby
S
Williams
Department of Ecology, Evolution, and Organismal Biology, Iowa State University, Ames, IA 50011, USA.
Shikha
S
Parsai
Department of Ecology, Evolution, and Organismal Biology, Iowa State University, Ames, IA 50011, USA.
Whitney
W
Manhart
Centro Nacional Patagónico, CONICET, Puerto Madryn, Chubut 9120, Argentina.
Maria G
MG
Palacios
D000064:Acclimatization; D000818:Animals; D000835:Animals, Wild / Q000254:growth & development / Q000502:physiology*; D000836:Animals, Zoo / Q000254:growth & development / Q000502:physiology*; D000910:Antibodies, Heterophile / Q000032:analysis; D015415:Biomarkers / Q000097:blood; D002140:California; D017812:Colubridae / Q000254:growth & development / Q000502:physiology*; D003345:Corticosterone / Q000097:blood; D005260:Female; D065948:Grassland; D060106:Lakes; D007958:Leukocyte Count / Q000662:veterinary; D018655:Lymphocyte Count / Q000662:veterinary; D008297:Male; D009504:Neutrophils / Q000276:immunology; D011247:Pregnancy; D015203:Reproducibility of Results; D012621:Seasons; D013312:Stress, Physiological
D000910:Antibodies, Heterophile; D015415:Biomarkers; D003345:Corticosterone
Captivity; Corticosterone; Leukocyte ratios; Stress; Thamnophis elegans