A reusable evanescent wave immunosensor for highly sensitive detection of bisphenol A in water samples.
This paper proposed a compact and portable planar waveguide evanescent wave immunosensor (EWI) for highly sensitive detection of BPA. The incident light is coupled into the planar waveguide chip via a beveled angle through undergoing total internal reflection, where the evanescent wave field forms and excites the binding fluorophore-tagged antibodies on the chip surface. Typical calibration curves obtained for BPA has detection limits of 0.03 μg/L. Linear response for BPA ranged from 0.124 μg/L-9.60 μg/L with 50% inhibition concentration for BPA of 1.09 ± 0.25 μg/L. The regeneration of the planar optical waveguide chip allows the performance of more than 300 assay cycles within an analysis time of about 20 min for each assay cycle. By application of effective pretreatment procedure, the recoveries of BPA in real water samples gave values from 88.3% ± 8.5% to 103.7% ± 3.5%, confirming its application potential in the measurement of BPA in reality.
1] State Key Joint Laboratory of ESPC, School of Environment, Tsinghua University, Beijing 10084, China [2] Collaborative Innovation Center for Advanced Water Pollution Control Technology and Equipment, Nanjing, China.
Zhou
Z
Xiao-Hong
State Key Joint Laboratory of ESPC, School of Environment, Tsinghua University, Beijing 10084, China.
Liu
L
Lan-Hua
State Key Joint Laboratory of ESPC, School of Environment, Tsinghua University, Beijing 10084, China.
Xu
X
Wei-Qi
State Key Joint Laboratory of ESPC, School of Environment, Tsinghua University, Beijing 10084, China.
Song
S
Bao-Dong
State Key Joint Laboratory of ESPC, School of Environment, Tsinghua University, Beijing 10084, China.
Sheng
S
Jian-Wu
1] State Key Joint Laboratory of ESPC, School of Environment, Tsinghua University, Beijing 10084, China [2] Collaborative Innovation Center for Advanced Water Pollution Control Technology and Equipment, Nanjing, China.
He
H
Miao
1] State Key Joint Laboratory of ESPC, School of Environment, Tsinghua University, Beijing 10084, China [2] Collaborative Innovation Center for Advanced Water Pollution Control Technology and Equipment, Nanjing, China.
Shi
S
Han-Chang
D000906:Antibodies / Q000737:chemistry; D001559:Benzhydryl Compounds / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D002138:Calibration; D007118:Immunoassay / Q000379:methods*; D010636:Phenols / Q000737:chemistry*; D012680:Sensitivity and Specificity; D014867:Water / Q000032:analysis* / Q000737:chemistry*; D000098826:Bisphenol A Compounds
D000906:Antibodies; D001559:Benzhydryl Compounds; D010636:Phenols; D014867:Water; C006780:bisphenol A; D000098826:Bisphenol A Compounds