ELIC-α7 Nicotinic acetylcholine receptor (α7nAChR) chimeras reveal a prominent role of the extracellular-transmembrane domain interface in allosteric modulation.
The native α7 nicotinic acetylcholine receptor (α7nAChR) is a homopentameric ligand-gated ion channel mediating fast synaptic transmission and is of pharmaceutical interest for treatment of numerous disorders. The transmembrane domain (TMD) of α7nAChR has been identified as a target for positive allosteric modulators (PAMs), but it is unclear whether modulation occurs through changes entirely within the TMD or changes involving both the TMD and the extracellular domain (ECD)-TMD interface. In this study, we constructed multiple chimeras using the TMD of human α7nAChR and the ECD of a prokaryotic homolog, ELIC, which is not sensitive to these modulators, and for which a high resolution structure has been solved. Functional ELIC-α7nAChR (EA) chimeras were obtained when their ECD-TMD interfaces were modified to resemble either the ELIC interface (EAELIC) or α7nAChR interface (EAα7). Both EAα7 and EAELIC show similar activation response and desensitization characteristics, but only EAα7 retained the unique pharmacology of α7nAChR evoked by PAMs, including potentiation by ivermectin, PNU-120596, and TQS, as well as activation by 4BP-TQS. This study suggests that PAM modulation through the TMD has a more stringent requirement at the ECD-TMD interface than agonist activation.
From the Departments of Anesthesiology.
Tommy S
TS
Tillman
From the Departments of Anesthesiology.
Edom
E
Seyoum
From the Departments of Anesthesiology, Computational and Systems Biology, and.
David D
DD
Mowrey
From the Departments of Anesthesiology, Pharmacology and Chemical Biology, Structural Biology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15260.
Yan
Y
Xu
From the Departments of Anesthesiology, Computational and Systems Biology, and Pharmacology and Chemical Biology, tangp@upmc.edu.
Pei
P
Tang
D000494:Allosteric Regulation; D000595:Amino Acid Sequence; D002462:Cell Membrane / Q000378:metabolism*; D005110:Extracellular Space / Q000378:metabolism*; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D015202:Protein Engineering; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D064569:alpha7 Nicotinic Acetylcholine Receptor / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
D011993:Recombinant Fusion Proteins; D064569:alpha7 Nicotinic Acetylcholine Receptor
Allosteric Regulation; ELIC; Ion Channels; Neurotransmitter Receptors; Nicotinic Acetylcholine Receptors; Pentameric Ligand-gated Ion Channels; Protein Chimeras; pLGICs; α7nAChR