MiR-324-5p inhibits proliferation of glioma by target regulation of GLI1.
OBJECTIVE
To study the effects of the miR-324-5p on the glioma cells proliferation via the targeted regulation of the glioma-associated oncogene 1.


METHODS
The luciferase reporter gene was used to test whether the glioma-associated oncogene 1 was the target of the miR-324-5p microRNA. The glioma-associated oncogene 1 expression was detected by Western blot. The proliferation and cell cycle were evaluated by MTT assay and flow cytometry.


RESULTS
The glioma-associated oncogene 1 is a target of the miR-324-5p. An over-expressed miR-324-5p could reduce the cell survival rate and increase the G1/G0 phase rate in the glioma cell lines.


CONCLUSIONS
The miR-324-5p can inhibit proliferation of the glioma cells via the targeted regulation of the glioma-associated oncogene 1.
Department of Neurosurgery, the Central Hospital of Xuzhou, Xuzhou Clinical School of Xuzhou Medical College, Affiliated Hospital of Southeast University, Xuzhou, Jiangsu, People's Republic of China. xhs51@126.com.
H-S
HS
Xu
H-L
HL
Zong
M
M
Shang
X
X
Ming
J-P
JP
Zhao
C
C
Ma
L
L
Cao
D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics*; D002470:Cell Survival / Q000235:genetics; D016193:G1 Phase / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D005910:Glioma / Q000235:genetics*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D016192:Resting Phase, Cell Cycle / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics*; D000071676:Zinc Finger Protein GLI1
C400551:GLI1 protein, human; D035683:MicroRNAs; D014157:Transcription Factors; D000071676:Zinc Finger Protein GLI1