Covalent immobilization of hLf1-11 peptide on a titanium surface reduces bacterial adhesion and biofilm formation.
Bacterial infection represents a major cause of implant failure in dentistry. A common approach to overcoming this issue and treating peri-implant infection consists in the use of antibiotics. However, the rise of multidrug-resistant bacteria poses serious concerns to this strategy. A promising alternative is the use of antimicrobial peptides due to their broad-spectrum activity against bacteria and reduced bacterial resistance responses. The aim of the present study was to determine the in vitro antibacterial activity of the human lactoferrin-derived peptide hLf1-11 anchored to titanium surfaces. To this end, titanium samples were functionalized with the hLf1-11 peptide either by silanization methods or physical adsorption. X-ray photoelectron spectroscopy analyses confirmed the successful covalent attachment of the hLf1-11 peptide onto titanium surfaces. Lactate dehydrogenase assay determined that hLf1-11 peptide did not affect fibroblast viability. An outstanding reduction in the adhesion and early stages of biofilm formation of Streptococcus sanguinis and Lactobacillus salivarius was observed on the biofunctionalized surfaces compared to control non-treated samples. Furthermore, samples coated with the hLf1-11 peptide inhibited the early stages of bacterial growth. Thus, this strategy holds great potential to develop antimicrobial biomaterials for dental applications.
Biomaterials, Biomechanics and Tissue Engineering Group, Department of Materials Science and Metallurgy, Technical University of Catalonia (UPC), ETSEIB, Av. Diagonal 647, 08028 Barcelona, Spain; Biomedical Research Networking Centre in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Campus Río Ebro, Edificio I+D Bloque 5, 1ª planta, C/ Poeta Mariano Esquillor s/n, 50018 Zaragoza, Spain; Centre for Research in NanoEngineering (CRNE) - UPC, C/Pascual i Vila 15, 08028 Barcelona, Spain. Electronic address: maria.godoy.gallardo@upc.edu.
Maria
M
Godoy-Gallardo
Biomaterials, Biomechanics and Tissue Engineering Group, Department of Materials Science and Metallurgy, Technical University of Catalonia (UPC), ETSEIB, Av. Diagonal 647, 08028 Barcelona, Spain; Biomedical Research Networking Centre in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Campus Río Ebro, Edificio I+D Bloque 5, 1ª planta, C/ Poeta Mariano Esquillor s/n, 50018 Zaragoza, Spain; Centre for Research in NanoEngineering (CRNE) - UPC, C/Pascual i Vila 15, 08028 Barcelona, Spain.
Carlos
C
Mas-Moruno
Biomedical Research Networking Centre in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Campus Río Ebro, Edificio I+D Bloque 5, 1ª planta, C/ Poeta Mariano Esquillor s/n, 50018 Zaragoza, Spain; Department of Biomedical Science, University of Extremadura, Av. de Elvas s/n, 06071 Badajoz, Spain.
María C
MC
Fernández-Calderón
Biomedical Research Networking Centre in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Campus Río Ebro, Edificio I+D Bloque 5, 1ª planta, C/ Poeta Mariano Esquillor s/n, 50018 Zaragoza, Spain; Department of Biomedical Science, University of Extremadura, Av. de Elvas s/n, 06071 Badajoz, Spain.
Ciro
C
Pérez-Giraldo
Biomaterials, Biomechanics and Tissue Engineering Group, Department of Materials Science and Metallurgy, Technical University of Catalonia (UPC), ETSEIB, Av. Diagonal 647, 08028 Barcelona, Spain; Biomedical Research Networking Centre in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Campus Río Ebro, Edificio I+D Bloque 5, 1ª planta, C/ Poeta Mariano Esquillor s/n, 50018 Zaragoza, Spain.
José M
JM
Manero
Institute for Research un Biomedicine (IRB-Barcelona), Barcelona Science Park, C/Baldiri Reixac 10, 08028 Barcelona, Spain; Department of Organic Chemistry, University of Barcelona, C/Martí i Franquès 1-11, 08028 Barcelona, Spain; School of Chemistry & Physics, University of KwaZulu-Natal, 4001 Durban, South Africa.
Fernando
F
Albericio
Biomaterials, Biomechanics and Tissue Engineering Group, Department of Materials Science and Metallurgy, Technical University of Catalonia (UPC), ETSEIB, Av. Diagonal 647, 08028 Barcelona, Spain; Biomedical Research Networking Centre in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Campus Río Ebro, Edificio I+D Bloque 5, 1ª planta, C/ Poeta Mariano Esquillor s/n, 50018 Zaragoza, Spain; Centre for Research in NanoEngineering (CRNE) - UPC, C/Pascual i Vila 15, 08028 Barcelona, Spain.
Francisco J
FJ
Gil
Biomaterials, Biomechanics and Tissue Engineering Group, Department of Materials Science and Metallurgy, Technical University of Catalonia (UPC), ETSEIB, Av. Diagonal 647, 08028 Barcelona, Spain; Biomedical Research Networking Centre in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Campus Río Ebro, Edificio I+D Bloque 5, 1ª planta, C/ Poeta Mariano Esquillor s/n, 50018 Zaragoza, Spain; Centre for Research in NanoEngineering (CRNE) - UPC, C/Pascual i Vila 15, 08028 Barcelona, Spain.
Daniel
D
Rodríguez
D000327:Adsorption; D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology; D001422:Bacterial Adhesion / Q000187:drug effects / Q000502:physiology*; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development*; D002470:Cell Survival / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis* / Q000494:pharmacology*; D007781:Lactoferrin / Q000737:chemistry / Q000494:pharmacology*; D008422:Materials Testing; D010446:Peptide Fragments / Q000737:chemistry / Q000494:pharmacology*; D011485:Protein Binding; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
D000890:Anti-Infective Agents; D020099:Coated Materials, Biocompatible; D010446:Peptide Fragments; C466658:lactoferrin (1-11), human; D014025:Titanium; D007781:Lactoferrin
Antimicrobial peptide; Bacterial adhesion; Biofunctionalization; Lactoferrin peptide; Titanium