Studies of genes involved in craniofacial development and tumorigenesis: FGF3 contributes to isolated oral clefts and may interact with PAX9.
OBJECTIVE
Previous studies suggest individuals born with oral clefts and their families have a higher susceptibility for cancer, which raises the hypothesis that these two conditions share common molecular pathways. This study evaluated the association between oral clefts and polymorphisms in genes that play a role in craniofacial and tumor development.


METHODS
Four hundred and ninety-seven subjects born with oral clefts and 823 unaffected subjects were recruited. Twenty-nine markers in 13 genes were genotyped by the Taqman method. Chi-square was used to compare allele and genotype frequencies. Bonferroni correction for multiple testing was used and the established alpha was 0.0003. This study also used logistic regression to test if genetic variants were associated with oral clefts using positive family history of cancer and age as covariates.


RESULTS
There was no association between family history of cancer and oral clefts (p = 0.51). None of the 1320 study participants had a diagnosis of cancer at the time of participation in the study. The marker rs4980700 in FGF3 was associated with oral clefts (p = 0.0002). Logistic regression analysis also provided evidence for gene-gene interaction between FGF3 (rs4980700) and PAX9 (rs2073242), increasing the risk for isolated oral clefts (p = 0.0003).


CONCLUSIONS
FGF3 is associated with oral clefts and may interact with PAX9.
Department of Oral Biology, School of Dental Medicine, University of Pittsburgh , Pittsburgh, PA , USA.
Erika C
EC
Küchler
Ticiana M
TM
Sabóia
Thays C
TC
Vieira
Andrea
A
Lips
Patricia N
PN
Tannure
Kathleen
K
Deeley
Maria F
MF
Reis
Bao
B
Ho
Ana C
AC
Rey
Marcelo C
MC
Costa
José M
JM
Granjeiro
Alexandre R
AR
Vieira
D000293:Adolescent; D000328:Adult; D000368:Aged; D063646:Carcinogenesis / Q000235:genetics*; D002648:Child; D002675:Child, Preschool; D002971:Cleft Lip / Q000235:genetics*; D002972:Cleft Palate / Q000235:genetics*; D004843:Epistasis, Genetic / Q000235:genetics; D005260:Female; D051518:Fibroblast Growth Factor 3 / Q000235:genetics*; D005787:Gene Frequency / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D014644:Genetic Variation / Q000235:genetics; D005838:Genotype; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D051764:PAX9 Transcription Factor / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000235:genetics; D055815:Young Adult
C496444:FGF3 protein, human; D051518:Fibroblast Growth Factor 3; D051764:PAX9 Transcription Factor; C497323:PAX9 protein, human
cancer; cleft lip; cleft palate; genetics