Comparison of the effects of high energy carbon heavy ion irradiation and Eucommia ulmoides Oliv. on biosynthesis butyric acid efficiency in Clostridium tyrobutyricum.
Clostridium tyrobutyricum is well documented as a fermentation strain for the production of butyric acid. In this work, using high-energy carbon heavy ion irradiated C. tyrobutyricum, then butyric acid fermentation using glucose or alkali and acid pretreatments of Eucommia ulmoides Oliv. as a carbon source was carried out. Initially, the modes at pH 5.7-6.5 and 37°C were compared using a model medium containing glucose as a carbon source. When the 72gL(-1) glucose concentration was found to be the highest yield, the maximum butyric acid production from glucose increased significantly, from 24gL(-1) for the wild type strains to 37gL(-1) for the strain irradiated at 126AMeV and a dose of 35Gy and a 10(7)ions/pulse. By feeding 100gL(-1) acid pretreatments of E. ulmoides Oliv. into the fermentations, butyrate yields (5.8gL(-1)) and butyrate/acetate (B/A) ratio (4.32) were achieved.
Institute of Modern Physics, Chinese Academy of Sciences, 509 Nanchang Rd., Lanzhou, Gansu 730000, PR China. Electronic address: syannovich@gmail.com.
Xiang
X
Zhou
Institute of Modern Physics, Chinese Academy of Sciences, 509 Nanchang Rd., Lanzhou, Gansu 730000, PR China.
Shu-Yang
SY
Wang
Institute of Modern Physics, Chinese Academy of Sciences, 509 Nanchang Rd., Lanzhou, Gansu 730000, PR China.
Xi-Hong
XH
Lu
Institute of Modern Physics, Chinese Academy of Sciences, 509 Nanchang Rd., Lanzhou, Gansu 730000, PR China. Electronic address: liangjp@hotmail.com.
Jian-Ping
JP
Liang
D020148:Butyric Acid / Q000378:metabolism*; D002244:Carbon; D048014:Clostridium tyrobutyricum / Q000502:physiology* / Q000528:radiation effects; D031283:Eucommiaceae / Q000378:metabolism*; D005240:Feasibility Studies; D005285:Fermentation; D020450:Heavy Ions
D020148:Butyric Acid; D002244:Carbon
(12)C(6+) heavy ion; Butyric acid; Clostridium tyrobutyricum; Eucommia ulmoides Oliv.; Fermentation