Global DNA methylation: uncommon event in oral lichenoid disease.
OBJECTIVE
Accumulating evidence indicates that aberrant DNA methylation is closely related to oral carcinogenesis, and it has been shown that methylation changes might be used as prognostic biomarker in oral squamous cell carcinoma. Oral lichenoid disease (OLD) is the most common oral potentially malignant disorder in our region. The aim of this study was to perform the first wide DNA methylation study in OLD in order to investigate the relevance of DNA methylation changes in this premalignant disorder.


METHODS
Two different Illumina microarray platforms, namely the GoldenGate Cancer Panel I and the HumanMethylation27 DNA Analysis BeadChip, were utilized in the discovery phase to interrogate the methylation profile of 59 OLD cases and 9 healthy individuals. Top-ranked genes were further validated by pyrosequencing in a second sample set consisting of 160 OLD and 65 controls.


RESULTS
Our results show that the frequency of aberrant DNA methylation is rare in OLD, and this finding was further corroborated by pyrosequencing in the biological validation.


CONCLUSIONS
These findings reinforce the notion that molecular alterations associated with oral carcinogenesis do not seem to be common events in OLD, which in turn might explain the low rate of malignization of this disorder.
Oral Medicine & Pathology, Department of Stomatology II, UFI 11/25, University of the Basque Country (UPV/EHU), Leioa, Spain; BIOMICs Research Group, University of the Basque Country (UPV/EHU), Vitoria-Gasteiz, Spain.
N G
NG
Bediaga
X
X
Marichalar-Mendia
J M
JM
Aguirre-Urizar
B
B
Calvo
M A
MA
Echebarria-Goicouria
M M
MM
de Pancorbo
A
A
Acha-Sagredo
D019175:DNA Methylation; D005260:Female; D006801:Humans; D017676:Lichen Planus, Oral / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009067:Mouthwashes; D011401:Promoter Regions, Genetic
D009067:Mouthwashes
genome-wide DNA methylation; oral lichenoid disease