High-purity separation of cancer cells by optically induced dielectrophoresis.
Detecting and concentrating cancer cells in peripheral blood is of great importance for cancer diagnosis and prognosis. Optically induced dielectrophoresis (ODEP) can achieve high resolution and low optical intensities, and the electrode pattern can be dynamically changed by varied light patterns. By changing the projected light pattern, it is demonstrated to separate high-purity gastric cancer cell lines. Traditionally, the purity of cancer cell isolation by negative selection is 0.9% to 10%; by positive selection it is 50% to 62%. An ODEP technology is proposed to enhance the purity of cancer cell isolation to about 77%.
Industrial Technology Research Institute, P.O. Box 31054, Room 308, Building 78, 195, Section 4, Chung Hsing Road, Chutung, Hsinchu, Taiwan.
Hsiu-Hsiang
HH
Chen
Industrial Technology Research Institute, P.O. Box 31054, Room 308, Building 78, 195, Section 4, Chung Hsing Road, Chutung, Hsinchu, Taiwan.
Mai-Wei
MW
Lin
Industrial Technology Research Institute, P.O. Box 31054, Room 308, Building 78, 195, Section 4, Chung Hsing Road, Chutung, Hsinchu, Taiwan.
Wan-Ting
WT
Tien
Industrial Technology Research Institute, P.O. Box 31054, Room 308, Building 78, 195, Section 4, Chung Hsing Road, Chutung, Hsinchu, Taiwan.
Chin-Pen
CP
Lai
Industrial Technology Research Institute, P.O. Box 31054, Room 308, Building 78, 195, Section 4, Chung Hsing Road, Chutung, Hsinchu, Taiwan.
Kuo-Yao
KY
Weng
Industrial Technology Research Institute, P.O. Box 31054, Room 308, Building 78, 195, Section 4, Chung Hsing Road, Chutung, Hsinchu, Taiwan.
Ching-Huai
CH
Ko
Industrial Technology Research Institute, P.O. Box 31054, Room 308, Building 78, 195, Section 4, Chung Hsing Road, Chutung, Hsinchu, Taiwan.
Chun-Chuan
CC
Lin
Industrial Technology Research Institute, P.O. Box 31054, Room 308, Building 78, 195, Section 4, Chung Hsing Road, Chutung, Hsinchu, Taiwan.
Jyh-Chern
JC
Chen
Industrial Technology Research Institute, P.O. Box 31054, Room 308, Building 78, 195, Section 4, Chung Hsing Road, Chutung, Hsinchu, Taiwan.
Kuo-Tung
KT
Tiao
Chang Gung University, Chang Gung Memorial Hospital, P.O. Box 33305, 259 Wen-Hwa 1st Road, Kwei-Shan Tao-Yuan, Taiwan.
Tse-Ching
TC
Chen
Chang Gung University, Chang Gung Memorial Hospital, P.O. Box 33305, 259 Wen-Hwa 1st Road, Kwei-Shan Tao-Yuan, Taiwan.
Shin-Cheh
SC
Chen
Chang Gung University, Chang Gung Memorial Hospital, P.O. Box 33305, 259 Wen-Hwa 1st Road, Kwei-Shan Tao-Yuan, Taiwan.
Ta-Sen
TS
Yeh
Industrial Technology Research Institute, P.O. Box 31054, Room 308, Building 78, 195, Section 4, Chung Hsing Road, Chutung, Hsinchu, Taiwan.
Chieh-Fang
CF
Cheng
D045744:Cell Line, Tumor; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods*; D004586:Electrophoresis / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D006801:Humans; D061986:MCF-7 Cells; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D009369:Neoplasms; D061848:Optical Imaging / Q000295:instrumentation / Q000379:methods*