Effects of lipoic acid on immune function, the antioxidant defense system, and inflammation-related genes expression of broiler chickens fed aflatoxin contaminated diets.
This study was designed to evaluate the effect of low level of Aflatoxin B1 (AFB1) on oxidative stress, immune reaction and inflammation response and the possible ameliorating effects of dietary alpha-lipoic acid (α-LA) in broilers. Birds were randomly allocated into three groups and assigned to receive different diets: basal diet, diet containing 74 μg/kg AFB1, and 300 mg/kg α-LA supplementation in diet containing 74 μg/kg AFB1 for three weeks. The results showed that the serum levels of malondialdehyde, tumor necrosis factor alpha (TNFα) and interferon gamma (IFNγ) in the AFB1-treated group were significantly increased than the control group. In addition, the increased expressions of interleukin 6 (IL6), TNFα and IFNγ were observed in birds exposed to the AFB1-contaminated diet. These degenerative changes were inhibited by α-LA-supplement. The activities of total superoxide dismutase and glutathione peroxidase, the levels of humoral immunity, and the expressions of nuclear factor-κB p65 and heme oxygenase-1, however, were not affected by AFB1. The results suggest that α-LA alleviates AFB1 induced oxidative stress and immune changes and modulates the inflammatory response at least partly through changes in the expression of proinflammatory cytokines of spleen such as IL6 and TNFα in broiler chickens.
State Key Laboratory of Animal Nutrition, College of Animal Science & Technology, China Agricultural University, Beijing 100193, China. liyan-602@163.com.
Yan
Y
Li
State Key Laboratory of Animal Nutrition, College of Animal Science & Technology, China Agricultural University, Beijing 100193, China. maqiugang@cau.edu.cn.
Qiu-Gang
QG
Ma
State Key Laboratory of Animal Nutrition, College of Animal Science & Technology, China Agricultural University, Beijing 100193, China. lihongzhao100@126.com.
Li-Hong
LH
Zhao
Translational Medicine Lab, Chinese National Human Genome Center, Beijing 100176, China. huaweimda@126.com.
Hua
H
Wei
State Key Laboratory of Animal Nutrition, College of Animal Science & Technology, China Agricultural University, Beijing 100193, China. duanguoxiang2014@gmail.com.
Guo-Xiang
GX
Duan
State Key Laboratory of Animal Nutrition, College of Animal Science & Technology, China Agricultural University, Beijing 100193, China. futurezjy@sina.com.
Jian-Yun
JY
Zhang
State Key Laboratory of Animal Nutrition, College of Animal Science & Technology, China Agricultural University, Beijing 100193, China. jicheng@cau.edu.cn.
Cheng
C
Ji
D016604:Aflatoxin B1 / Q000008:administration & dosage / Q000633:toxicity*; D000821:Animal Feed; D000818:Animals; D000975:Antioxidants; D002645:Chickens; D004032:Diet; D019587:Dietary Supplements; D005979:Glutathione Peroxidase / Q000096:biosynthesis; D051547:Heme Oxygenase-1 / Q000096:biosynthesis; D007249:Inflammation / Q000235:genetics / Q000276:immunology*; D007371:Interferon-gamma / Q000096:biosynthesis / Q000097:blood; D015850:Interleukin-6 / Q000096:biosynthesis; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D018384:Oxidative Stress / Q000187:drug effects*; D012333:RNA, Messenger / Q000096:biosynthesis; D011897:Random Allocation; D013482:Superoxide Dismutase / Q000096:biosynthesis; D008063:Thioctic Acid / Q000494:pharmacology*; D051996:Transcription Factor RelA / Q000096:biosynthesis; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000097:blood
D000975:Antioxidants; D015850:Interleukin-6; D012333:RNA, Messenger; D051996:Transcription Factor RelA; D014409:Tumor Necrosis Factor-alpha; D008315:Malondialdehyde; D008063:Thioctic Acid; D007371:Interferon-gamma; D016604:Aflatoxin B1; D005979:Glutathione Peroxidase; D051547:Heme Oxygenase-1; D013482:Superoxide Dismutase