Water extract of Acer tegmentosum reduces bone destruction by inhibiting osteoclast differentiation and function.
The stem of Acer tegmentosum has been widely used in Korea for the treatment of hepatic disorders. In this study, we investigated the bone protective effect of water extract of the stem of Acer tegmentosum (WEAT). We found that WEAT inhibits osteoclast differentiation induced by receptor activator of nuclear factor-κB ligand (RANKL), an essential cytokine for osteoclast differentiation. In osteoclast precursor cells, WEAT inhibited RANKL-induced activation of JNK, NF-κB, and cAMP response element-binding protein, leading to suppression of the induction of c-Fos and nuclear factor of activated T cells cytoplasmic 1, key transcription factors for osteoclast differentiation. In addition, WEAT inhibited bone resorbing activity of mature osteoclasts. Furthermore, the oral administration of WEAT reduced RANKL-induced bone resorption and trabecular bone loss in mice. Taken together, our study demonstrates that WEAT possesses a protective effect on bone destruction by inhibiting osteoclast differentiation and function.
KM-Based Herbal Drug Development Group, Korea Institute of Oriental Medicine, Daejeon 305-811, Korea.
Hyunil
H
Ha
KM-Based Herbal Drug Development Group, Korea Institute of Oriental Medicine, Daejeon 305-811, Korea.
Ki-Shuk
KS
Shim
KM-Based Herbal Drug Development Group, Korea Institute of Oriental Medicine, Daejeon 305-811, Korea.
Taesoo
T
Kim
KM-Based Herbal Drug Development Group, Korea Institute of Oriental Medicine, Daejeon 305-811, Korea.
Hyosun
H
An
KM-Based Herbal Drug Development Group, Korea Institute of Oriental Medicine, Daejeon 305-811, Korea.
Chung-Jo
CJ
Lee
KM-Based Herbal Drug Development Group, Korea Institute of Oriental Medicine, Daejeon 305-811, Korea.
Kwang Jin
KJ
Lee
KM-Based Herbal Drug Development Group, Korea Institute of Oriental Medicine, Daejeon 305-811, Korea. jyma@kiom.re.kr.
Jin Yeul
JY
Ma
D031002:Acer / Q000737:chemistry*; D000284:Administration, Oral; D000818:Animals; D050071:Bone Density Conservation Agents / Q000737:chemistry / Q000494:pharmacology*; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D001862:Bone Resorption / Q000517:prevention & control*; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D017362:Cyclic AMP Response Element-Binding Protein / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D048670:MAP Kinase Kinase 4 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D050778:NFATC Transcription Factors / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D010010:Osteoclasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018547:Plant Stems / Q000737:chemistry; D016760:Proto-Oncogene Proteins c-fos / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D053245:RANK Ligand / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D014867:Water
D050071:Bone Density Conservation Agents; C492004:Creb1 protein, mouse; D017362:Cyclic AMP Response Element-Binding Protein; D050778:NFATC Transcription Factors; D010936:Plant Extracts; D016760:Proto-Oncogene Proteins c-fos; D053245:RANK Ligand; C505689:Tnfsf11 protein, mouse; D014867:Water; D048670:MAP Kinase Kinase 4