A multiplex biomarker approach for the diagnosis of transitional cell carcinoma from canine urine.
Transitional cell carcinoma (TCC), the most common cancer of the urinary bladder in dogs, is usually diagnosed at an advanced disease stage with limited response to chemotherapy. Commercial screening tests lack specificity and current diagnostic procedures are invasive. A proof of concept pilot project for analyzing the canine urinary proteome as a noninvasive diagnostic tool for TCC identification was conducted. Urine was collected from 12 dogs in three cohorts (healthy, urinary tract infection, TCC) and analyzed using liquid chromatography tandem mass spectrometry. The presence of four proteins (macrophage capping protein, peroxiredoxin 5, heterogeneous nuclear ribonucleoproteins A2/B, and apolipoprotein A1) was confirmed via immunoblot. Of the total 379 proteins identified, 96 were unique to the TCC group. A statistical model, designed to evaluate the accuracy of this multiplex biomarker approach for diagnosis of TCC, predicted the presence of disease with 90% accuracy.
Department of Clinical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR 97331, USA. Electronic address: shay.bracha@oregonstate.edu.
Shay
S
Bracha
EACRI, Providence Portland Medical Center, Portland, OR 97213, USA.
Michael
M
McNamara
Department of Clinical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR 97331, USA.
Ian
I
Hilgart
Department of Clinical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR 97331, USA.
Milan
M
Milovancev
Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR 97331, USA.
Jan
J
Medlock
Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR 97331, USA.
Cheri
C
Goodall
Department of Chemistry, Oregon State University, Corvallis, OR 97331, USA.
Samanthi
S
Wickramasekara
Department of Chemistry, Oregon State University, Corvallis, OR 97331, USA.
Claudia S
CS
Maier
D000595:Amino Acid Sequence; D000818:Animals; D016632:Apolipoprotein A-I / Q000652:urine; D001424:Bacterial Infections / Q000652:urine / Q000662:veterinary; D014408:Biomarkers, Tumor / Q000652:urine*; D002295:Carcinoma, Transitional Cell / Q000652:urine* / Q000662:veterinary*; D016022:Case-Control Studies; D002853:Chromatography, Liquid / Q000379:methods; D004283:Dog Diseases / Q000652:urine*; D004285:Dogs; D015151:Immunoblotting; D008969:Molecular Sequence Data; D054464:Peroxiredoxins / Q000652:urine; D010865:Pilot Projects; D016133:Polymerase Chain Reaction / Q000379:methods; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*
D016632:Apolipoprotein A-I; D014408:Biomarkers, Tumor; D054464:Peroxiredoxins
Biomarkers; Canine; Liquid chromatography; Tandem mass spectrometry; Transitional cell carcinoma