A roadmap to applying optogenetics in neuroscience.
Optogenetics allows for the specific manipulation of the activity of genetically defined cell populations in the CNS. Yet, it requires effective gene delivery, light stimulation, and readout strategies. Here, we provide a roadmap aimed at guiding the experimenter in the process of establishing an optogenetic approach tailored to a given research hypothesis in the field of neuroscience.
Focus Program Translational Neuroscience (FTN) and Institute for Microscopic Anatomy and Neurobiology, Johannes Gutenberg University Mainz, Hanns-Dieter-Hüsch-Weg 19, 55128, Mainz, Germany.
Consuelo
C
Fois
Pierre-Hugues
PH
Prouvot
Albrecht
A
Stroh
D000818:Animals; D001522:Behavior, Animal; D001921:Brain / Q000378:metabolism; D005822:Genetic Vectors; D006801:Humans; D015640:Ion Channel Gating / Q000528:radiation effects; D008027:Light; D009488:Neurosciences; D062308:Optogenetics; D055668:Photochemical Processes; D012243:Rhodopsin / Q000096:biosynthesis / Q000235:genetics; D015533:Transcriptional Activation / Q000528:radiation effects; D014161:Transduction, Genetic
D012243:Rhodopsin