Breast carcinoma progression and tumour vascular markers related to apoptotic mechanisms.
BACKGROUND
In the last few years, the cancer research had tried to identify and characterize new biochemical and molecular pathways in which the inhibition induces prosurvival mechanisms. Our work describes the expression of two different members of apoptotic regulatory pathway and their relationship with a progression of breast carcinoma.


METHODS
We compared expression of genes related to apoptosis (DR6 and Gpm6B) in the blood of patients suffering from stage I of breast cancer in different grades (I-IV), with healthy controls. After isolation of mRNA, transcription of mRNA into the cDNA was performed. The quantification of gene expression changes in DR6 and Gpm6B was detected by RT-PCR method. Analysis at the protein level was performed by the Western blot.


RESULTS
In statistical analysis of Dr6 mRNA level changes we detected significant increase starting in Grading 1 (G1) and reached maximal level in G3.This expression on mRNA levels was similar to protein levels, which copy rising tendency with maximal value in G3. The results of Gpm6B were significantly lower.


CONCLUSIONS
This result showed that antiapoptotic signalling during neovascularization is increased significantly. It would be advisable in the future to study the influence of cytostatic treatment on the expression of genes related to apoptotic pathways and their relationship with progression of breast cancer tumours.
Department of Medical and Clinical Biochemistry and LABMED, Faculty of Medicine, P. J. Šafárik University in Košice, Trieda SNP 1, 040 11 Košice, Slovakia.
Miroslava
M
Bilecova-Rabajdova
Department of Medical and Clinical Biochemistry and LABMED, Faculty of Medicine, P. J. Šafárik University in Košice, Trieda SNP 1, 040 11 Košice, Slovakia.
Peter
0000-0003-2749-7953
P
Urban
Department of Histology and Embryology, Faculty of Medicine, P. J. Šafárik University in Košice, Šrobárova 2, 040 11 Košice, Slovakia.
Kristina
K
Gregova
2nd Department of Gynecology and Obstetrics, Faculty of Medicine, P. J. Šafárik University in Košice, Rastislavova 43, 040 11 Košice, Slovakia.
Jan
J
Varga
2nd Department of Gynecology and Obstetrics, Faculty of Medicine, P. J. Šafárik University in Košice, Rastislavova 43, 040 11 Košice, Slovakia.
Viera
V
Fialkovicová
International Clinical Research Center, St. Anne's University Hospital, Pekařská 53, 656 91 Brno, Czech Republic.
Peter
0000-0002-7069-3784
P
Kruzliak
Department of Medical and Clinical Biochemistry and LABMED, Faculty of Medicine, P. J. Šafárik University in Košice, Trieda SNP 1, 040 11 Košice, Slovakia.
Maria
M
Marekova
D000199:Actins / Q000235:genetics / Q000378:metabolism; D000328:Adult; D017209:Apoptosis; D014408:Biomarkers, Tumor / Q000097:blood* / Q000235:genetics; D001943:Breast Neoplasms / Q000097:blood* / Q000098:blood supply / Q000473:pathology; D018270:Carcinoma, Ductal, Breast / Q000097:blood* / Q000098:blood supply / Q000473:pathology; D018275:Carcinoma, Lobular / Q000097:blood / Q000098:blood supply / Q000473:pathology; D016022:Case-Control Studies; D018450:Disease Progression; D005260:Female; D015870:Gene Expression; D006801:Humans; D008562:Membrane Glycoproteins / Q000097:blood* / Q000235:genetics; D008875:Middle Aged; D060787:Neoplasm Grading; D009389:Neovascularization, Pathologic / Q000097:blood / Q000473:pathology; D009419:Nerve Tissue Proteins / Q000097:blood* / Q000235:genetics; D012333:RNA, Messenger / Q000097:blood / Q000235:genetics; D018124:Receptors, Tumor Necrosis Factor / Q000097:blood* / Q000235:genetics
D000199:Actins; D014408:Biomarkers, Tumor; C484160:GPM6B protein, human; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D018124:Receptors, Tumor Necrosis Factor; C114109:TNFRSF21 protein, human