Loading of bacterial nanocellulose hydrogels with proteins using a high-speed technique.
For the loading of the natural biopolymer bacterial nanocellulose (BNC) with drugs, usually an adsorption method has been described. In the present study, a high-speed loading technique based on vortexing was established for the incorporation of proteins in BNC as drug delivery system. Compared to the conventional technique, vortexing accomplished in 10 min the same protein loading capacity as the adsorption method in 24h with comparable protein distribution and protein stability. Vortex loaded BNC demonstrated a retarded protein release with a lower total amount of released protein after 168 h compared to the adsorption loaded BNC. This was correlated with a densification of the fiber network as shown by electron microscopy and a reduced water holding capacity. These observations offer the possibility to control the drug release by selection of the preparation technique.
Department of Pharmaceutical Technology, Institute of Pharmacy, Friedrich-Schiller-University, Otto-Schott-Strasse 41, 07745 Jena, Germany.
Astrid
A
Müller
Otto-Schott-Institute of Materials Research (OSIM), Friedrich-Schiller-University, Loebdergraben 32, 07743 Jena, Germany.
Falko
F
Wesarg
Department of Pharmaceutical Technology, Institute of Pharmacy, Friedrich-Schiller-University, Otto-Schott-Strasse 41, 07745 Jena, Germany.
Nadine
N
Hessler
Otto-Schott-Institute of Materials Research (OSIM), Friedrich-Schiller-University, Loebdergraben 32, 07743 Jena, Germany; Jena Center for Soft Matter (JCSM), Friedrich-Schiller-University, Humboldt-Strasse 10, 07743 Jena, Germany.
Frank A
FA
Müller
Department of Pharmaceutical Technology, Institute of Pharmacy, Friedrich-Schiller-University, Otto-Schott-Strasse 41, 07745 Jena, Germany; Jena Center for Soft Matter (JCSM), Friedrich-Schiller-University, Humboldt-Strasse 10, 07743 Jena, Germany.
Dana
D
Kralisch
Department of Pharmaceutical Technology, Institute of Pharmacy, Friedrich-Schiller-University, Otto-Schott-Strasse 41, 07745 Jena, Germany; Jena Center for Soft Matter (JCSM), Friedrich-Schiller-University, Humboldt-Strasse 10, 07743 Jena, Germany. Electronic address: dagmar.fischer@uni-jena.de.
Dagmar
D
Fischer
D016947:Acetobacteraceae / Q000737:chemistry*; D000327:Adsorption; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002417:Cattle; D002482:Cellulose / Q000737:chemistry* / Q000302:isolation & purification; D003692:Delayed-Action Preparations; D020100:Hydrogels / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D008855:Microscopy, Electron, Scanning; D057139:Nanofibers / Q000737:chemistry*; D012399:Rotation; D012710:Serum Albumin, Bovine / Q000032:analysis / Q000737:chemistry* / Q000493:pharmacokinetics; D014783:Viscosity
D001672:Biocompatible Materials; D003692:Delayed-Action Preparations; D020100:Hydrogels; D012710:Serum Albumin, Bovine; D002482:Cellulose
Bacterial nanocellulose; Controlled release; Hydrogel; Protein delivery