Isolation and characterization of novel lipase gene LipHim1 from the DNA isolated from soil samples.
Metagenomics is a magnificent tool to isolate genes from unknown/uncharacterized species and also from organisms that cannot be cultured. In this study, we constructed a metagenomic library from isolated DNA of soil samples collected from Palamuru University campus premises, in Mahabubnagar district of Andhra Pradesh, India. We isolated a novel lipase gene LipHim1, which has an open reading frame of 591 base pairs and encodes ∼23 kDa protein consisting of 196 amino acids. The Lipase LipHim1 showed maximum 32% homology at the protein level with the extracellular Aeromonas hydrophila lipase (Class II, GDSL family) and was significantly different from all other known lipases. The isolated lipase catalyzed the hydrolysis of fatty acid esters of polyoxyethylene sorbitan such as Tween 60. Our results indicate that the isolated lipase gene is novel.
Department of Microbiology, Palamuru University, Mahabubnagar, 509001, Andhra Pradesh, India, pavankumarpindi@gmail.com.
Pavan Kumar
PK
Pindi
Raja Srinath
RS
R
Theetha L
TL
Pavankumar
D016980:Aeromonas hydrophila / Q000201:enzymology / Q000235:genetics; D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D005227:Fatty Acids / Q000378:metabolism; D006868:Hydrolysis; D007194:India; D008049:Lipase / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D056186:Metagenomics / Q000379:methods; D008970:Molecular Weight; D016366:Open Reading Frames; D011136:Polysorbates / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D012988:Soil Microbiology
D005227:Fatty Acids; D011136:Polysorbates; D004247:DNA; D008049:Lipase