N-Cadherin and Tie2 positive CD34⁺CD38⁻CD123⁺ leukemic stem cell populations can develop acute myeloid leukemia more effectively in NOD/SCID mice.
Emerging studies suggest that the population of malignant cells found in human acute myelogenous leukemia (AML) arises from a rare population of leukemic stem cells (LSCs). A lot of investigators have reported the identification of cell surface markers, such as CD123. Here, we report the identification of N-cadherin and Tie2 as LSCs markers. Inoculation of CD34(+)CD38(-)CD123(+)N-cadherin(+) and CD34(+)CD38(-)CD123(+) Tie2(+) population can induce leukemia in NOD/SCID mice. The leukemic blast cells from the primary leukemic mice could also induce leukemia in the secondary transplantation. These findings suggested that N-cadherin and Tie2 were the important markers that can assist in leukemia development.
State Key Laboratory of Experiment Hematology, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Science & Peking Union Medical College (CAMS & PUMC), Tianjin 300020, China.
Shaowei
S
Qiu
State Key Laboratory of Experiment Hematology, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Science & Peking Union Medical College (CAMS & PUMC), Tianjin 300020, China.
Yujiao
Y
Jia
State Key Laboratory of Experiment Hematology, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Science & Peking Union Medical College (CAMS & PUMC), Tianjin 300020, China.
Haiyan
H
Xing
State Key Laboratory of Experiment Hematology, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Science & Peking Union Medical College (CAMS & PUMC), Tianjin 300020, China.
Tengteng
T
Yu
State Key Laboratory of Experiment Hematology, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Science & Peking Union Medical College (CAMS & PUMC), Tianjin 300020, China.
Jing
J
Yu
State Key Laboratory of Experiment Hematology, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Science & Peking Union Medical College (CAMS & PUMC), Tianjin 300020, China.
Pei
P
Yu
State Key Laboratory of Experiment Hematology, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Science & Peking Union Medical College (CAMS & PUMC), Tianjin 300020, China.
Kejing
K
Tang
State Key Laboratory of Experiment Hematology, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Science & Peking Union Medical College (CAMS & PUMC), Tianjin 300020, China.
Zheng
Z
Tian
State Key Laboratory of Experiment Hematology, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Science & Peking Union Medical College (CAMS & PUMC), Tianjin 300020, China.
Huijun
H
Wang
State Key Laboratory of Experiment Hematology, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Science & Peking Union Medical College (CAMS & PUMC), Tianjin 300020, China.
Yingchang
Y
Mi
State Key Laboratory of Experiment Hematology, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Science & Peking Union Medical College (CAMS & PUMC), Tianjin 300020, China.
Qing
Q
Rao
State Key Laboratory of Experiment Hematology, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Science & Peking Union Medical College (CAMS & PUMC), Tianjin 300020, China.
Min
M
Wang
State Key Laboratory of Experiment Hematology, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Science & Peking Union Medical College (CAMS & PUMC), Tianjin 300020, China. Electronic address: wangjx@ihcams.ac.cn.
Jianxiang
J
Wang
D051997:ADP-ribosyl Cyclase 1 / Q000032:analysis*; D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D018952:Antigens, CD34 / Q000032:analysis*; D015820:Cadherins / Q000032:analysis*; D005260:Female; D006801:Humans; D053650:Interleukin-3 Receptor alpha Subunit / Q000032:analysis*; D015470:Leukemia, Myeloid, Acute / Q000209:etiology* / Q000473:pathology; D008297:Male; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D008875:Middle Aged; D014411:Neoplastic Stem Cells / Q000737:chemistry / Q000166:cytology*; D042787:Receptor, TIE-2 / Q000032:analysis*
D018952:Antigens, CD34; D015820:Cadherins; C115022:IL3RA protein, human; D053650:Interleukin-3 Receptor alpha Subunit; D042787:Receptor, TIE-2; D051997:ADP-ribosyl Cyclase 1
Acute myeloid leukemia; Leukemic stem cell; N-Cadherin; Tie2