SRC-1 and Twist1 expression positively correlates with a poor prognosis in human breast cancer.
To evaluate the possible prognostic value of Steroid Receptor Coactivator-1 (SRC-1) and Twist1 expression in human breast cancer, we examined SRC-1 and Twist1 expression using immunohistochemistry on tissue microarray sections containing 137 breast cancer specimens. All patients were followed up for a median of 5 years following surgery. Survival curves were generated using the Kaplan-Meier method. Multivariate analysis was performed using the Cox proportional hazard regression model to assess the prognostic values. The results showed a positive correlation between SRC-1 and Twist1 expression at protein levels (P < 0.001). Also, SRC-1 expression positively correlated with HER2 expression (P = 0.024). The protein expression of Twist1 positively associated with lymph node metastasis (P < 0.001), but inversely correlated with PR status (P = 0.041). Patients with SRC-1 or Twist1-positive expression exhibited poorer overall survival (OS) and disease-free survival (DFS) than did those with SRC-1 or Twist1-negative expression (P < 0.05 for all). In addition, SRC-1-negativeive/Twist1-negative patients had the best OS and DFS (P < 0.01 for both). In multivariate survival analysis, SRC-1 expression, tumor stage, and PR were found to be independent prognostic factors related to OS (P = 0.019, < 0.001 and 0.02, respectively) and Twist1 expression, lymph node status and PR were independent predictors of DFS (P = 0.006, 0.001 and 0.029, respectively). These results suggest that a combined SRC-1/Twist1 expression status could improve the prognostic judgment for breast cancer patients.
1. Breast Disease Center, Southwest Hospital, Third Military Medical University, Chongqing, China.
Yan
Y
Xu
1. Breast Disease Center, Southwest Hospital, Third Military Medical University, Chongqing, China.
Baoquan
B
Hu
2. Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas.
Li
L
Qin
3. Department of Pathology, Daping Hospital, Third Military Medical University, Chongqing, China.
Lianhua
L
Zhao
4. Institute of Pathology and Southwest Cancer Center, Southwest Hospital, Third Military Medical University, Chongqing, China.
Qiang
Q
Wang
4. Institute of Pathology and Southwest Cancer Center, Southwest Hospital, Third Military Medical University, Chongqing, China.
Qingliang
Q
Wang
2. Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas.
Yixiang
Y
Xu
1. Breast Disease Center, Southwest Hospital, Third Military Medical University, Chongqing, China.
Jun
J
Jiang
D001943:Breast Neoplasms / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D015999:Multivariate Analysis; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D056920:Nuclear Receptor Coactivator 1 / Q000235:genetics / Q000378:metabolism*; D011379:Prognosis; D016019:Survival Analysis; D051879:Twist-Related Protein 1 / Q000235:genetics / Q000378:metabolism*
D009687:Nuclear Proteins; C103173:TWIST1 protein, human; D051879:Twist-Related Protein 1; C538859:NCOA1 protein, human; D056920:Nuclear Receptor Coactivator 1
Breast cancer; Correlation; Prognosis.; SRC-1; Twist1