Genomic and phenotypic characterization of Vibrio cholerae non-O1 isolates from a US Gulf Coast cholera outbreak.
Between November 2010, and May 2011, eleven cases of cholera, unrelated to a concurrent outbreak on the island of Hispaniola, were recorded, and the causative agent, Vibrio cholerae serogroup O75, was traced to oysters harvested from Apalachicola Bay, Florida. From the 11 diagnosed cases, eight isolates of V. cholerae were isolated and their genomes were sequenced. Genomic analysis demonstrated the presence of a suite of mobile elements previously shown to be involved in the disease process of cholera (ctxAB, VPI-1 and -2, and a VSP-II like variant) and a phylogenomic analysis showed the isolates to be sister taxa to toxigenic V. cholerae V51 serogroup O141, a clinical strain isolated 23 years earlier. Toxigenic V. cholerae O75 has been repeatedly isolated from clinical cases in the southeastern United States and toxigenic V. cholerae O141 isolates have been isolated globally from clinical cases over several decades. Comparative genomics, phenotypic analyses, and a Caenorhabditis elegans model of infection for the isolates were conducted. This analysis coupled with isolation data of V. cholerae O75 and O141 suggests these strains may represent an underappreciated clade of cholera-causing strains responsible for significant disease burden globally.
Maryland Pathogen Research Institute, University of Maryland, College Park, Maryland, United States of America.
Bradd J
BJ
Haley
CosmosID, College Park, Maryland, United States of America.
Seon Young
SY
Choi
Food and Drug Administration, USFDA/CFSAN/DVA, Laurel, Maryland, United States of America.
Christopher J
CJ
Grim
Florida Department of Health Bureau of Environmental Public Health Medicine, Tallahassee, Florida, United States of America.
Tiffiani J
TJ
Onifade
Food and Drug Administration, USFDA/CFSAN/DVA, Laurel, Maryland, United States of America.
Hediye N
HN
Cinar
Food and Drug Administration, USFDA/CFSAN/DVA, Laurel, Maryland, United States of America.
Ben D
BD
Tall
Maryland Pathogen Research Institute, University of Maryland, College Park, Maryland, United States of America.
Elisa
E
Taviani
Maryland Pathogen Research Institute, University of Maryland, College Park, Maryland, United States of America; CosmosID, College Park, Maryland, United States of America.
Nur A
NA
Hasan
CosmosID, College Park, Maryland, United States of America.
Abdulshakur H
AH
Abdullah
Food and Drug Administration, USFDA/CFSAN/DVA, Laurel, Maryland, United States of America.
Laurenda
L
Carter
Food and Drug Administration, USFDA/CFSAN/DVA, Laurel, Maryland, United States of America.
Surasri N
SN
Sahu
Food and Drug Administration, USFDA/CFSAN/DVA, Laurel, Maryland, United States of America.
Mahendra H
MH
Kothary
Maryland Pathogen Research Institute, University of Maryland, College Park, Maryland, United States of America.
Arlene
A
Chen
Florida Department of Health Bureau of Public Health Laboratories, Jacksonville, Florida, United States of America.
Ron
R
Baker
Florida Department of Health Bureau of Environmental Public Health Medicine, Tallahassee, Florida, United States of America.
Richard
R
Hutchinson
Florida Department of Health Bureau of Environmental Public Health Medicine, Tallahassee, Florida, United States of America.
Carina
C
Blackmore
CosmosID, College Park, Maryland, United States of America; Department of Biology, Johns Hopkins University, Baltimore, Maryland, United States of America.
Thomas A
TA
Cebula
Maryland Pathogen Research Institute, University of Maryland, College Park, Maryland, United States of America; Maryland Institute for Applied Environmental Health, School of Public Health, University of Maryland, College Park, Maryland, United States of America.
Anwar
A
Huq
Maryland Pathogen Research Institute, University of Maryland, College Park, Maryland, United States of America; CosmosID, College Park, Maryland, United States of America; University of Maryland Institute for Advanced Computer Studies, University of Maryland, College Park, Maryland, United States of America; Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland, United States of America.
Rita R
RR
Colwell
D000818:Animals; D001483:Base Sequence; D017173:Caenorhabditis elegans / Q000382:microbiology*; D002771:Cholera / Q000453:epidemiology* / Q000382:microbiology*; D004196:Disease Outbreaks; D023281:Genomics; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012689:Sequence Homology, Nucleic Acid; D014481:United States / Q000453:epidemiology; D044163:Vibrio cholerae non-O1 / Q000145:classification / Q000302:isolation & purification* / Q000502:physiology; D037521:Virulence Factors
D037521:Virulence Factors