Isolation of jasmonate-induced sesquiterpene synthase of rice: product of which has an antifungal activity against Magnaporthe oryzae.
Rice is one of the most important crops worldwide, and it is a model for molecular studies of monocotyledonous species, particularly for understanding the molecular mechanisms of plant disease resistance. Jasmonic acid (JA) is an important plant hormone involved in rice-pathogen interactions. In addition, JA-induced volatiles are known to be involved in the rice defense system regulated by JA signaling. In this study, we isolated a JA-induced terpene synthase from rice, and found that it produces two sesquiterpenes; β-elemene and β-bisabolene. Furthermore, β-elemene exhibited significant antifungal activity against Magnaporthe oryzae; however it did not exhibited any antibacterial activity against Xanthomonas oryzae pv. oryzae. JA-induced accumulation of β-elemene was regulated by OsJAZ8, a rice jasmonate ZIM-domain (JAZ) protein that is involved in the JA signaling pathway, suggesting that β-elemene plays an important role in JA-induced resistance, and that it functions as an antifungal compound in rice.
Faculty of Agriculture and Gene Research Center, Kagawa University, Miki, Kagawa 761-0795, Japan.
Shiduku
S
Taniguchi
Faculty of Agriculture and Gene Research Center, Kagawa University, Miki, Kagawa 761-0795, Japan.
Seika
S
Miyoshi
Faculty of Agriculture and Gene Research Center, Kagawa University, Miki, Kagawa 761-0795, Japan.
Daisuke
D
Tamaoki
Faculty of Agriculture and Gene Research Center, Kagawa University, Miki, Kagawa 761-0795, Japan.
Shoko
S
Yamada
Faculty of Agriculture and Gene Research Center, Kagawa University, Miki, Kagawa 761-0795, Japan.
Keiichiro
K
Tanaka
Faculty of Agriculture and Gene Research Center, Kagawa University, Miki, Kagawa 761-0795, Japan.
Yuya
Y
Uji
Faculty of Agriculture and Gene Research Center, Kagawa University, Miki, Kagawa 761-0795, Japan.
Suzumi
S
Tanaka
Faculty of Agriculture and Gene Research Center, Kagawa University, Miki, Kagawa 761-0795, Japan.
Kazuya
K
Akimitsu
Faculty of Agriculture and Gene Research Center, Kagawa University, Miki, Kagawa 761-0795, Japan. Electronic address: gomiken@ag.kagawa-u.ac.jp.
Kenji
K
Gomi
D019883:Alkyl and Aryl Transferases / Q000235:genetics / Q000378:metabolism; D000595:Amino Acid Sequence; D000935:Antifungal Agents / Q000494:pharmacology; D003517:Cyclopentanes / Q000378:metabolism / Q000494:pharmacology; D004926:Escherichia coli / Q000235:genetics; D008401:Gas Chromatography-Mass Spectrometry; D020082:Magnaporthe / Q000187:drug effects*; D008969:Molecular Sequence Data; D012275:Oryza / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000382:microbiology*; D054883:Oxylipins / Q000378:metabolism / Q000494:pharmacology; D010935:Plant Diseases / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014974:Xanthomonas / Q000187:drug effects
D000935:Antifungal Agents; D003517:Cyclopentanes; D054883:Oxylipins; D010940:Plant Proteins; C011006:jasmonic acid; D019883:Alkyl and Aryl Transferases; C108877:terpene synthase
Jasmonic acid; Magnaporthe oryzae; Plant volatile; Terpene synthase; β-Elemene