Sequence-level and dual-phase identification of Salmonella flagellum antigens by liquid chromatography-tandem mass spectrometry (LC-MS/MS).
Forty-three reference strains involving the 24 most common serovars of Salmonella enterica were examined by using a mass spectrometry-based H antigen typing platform (MS-H). The results indicate that MS-H can be used as a sensitive, rapid, and straightforward approach for the typing of Salmonella flagella at the molecular level without antiserum and phase inversion.
National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada Department of Human Anatomy and Cell Sciences, Faculty of Medicine, University of Manitoba, Winnipeg, Manitoba, Canada keding.cheng@phac-aspc.gc.ca gehua.wang@phac-aspc.gc.ca.
Keding
K
Cheng
National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
Angela
A
Sloan
National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
Julie
J
Meakin
National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
Stuart
S
McCorrister
National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
Morganne
M
Jerome
National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
Garrett
G
Westmacott
National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
Mike
M
Drebot
National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
Celine
C
Nadon
National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
J David
JD
Knox
National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada keding.cheng@phac-aspc.gc.ca gehua.wang@phac-aspc.gc.ca.
Gehua
G
Wang
D000942:Antigens, Bacterial / Q000737:chemistry*; D001431:Bacteriological Techniques / Q000379:methods; D002853:Chromatography, Liquid / Q000379:methods*; D005407:Flagella / Q000737:chemistry*; D006801:Humans; D019779:Salmonella enterica / Q000737:chemistry* / Q000145:classification*; D053719:Tandem Mass Spectrometry / Q000379:methods*
D000942:Antigens, Bacterial; C022863:H antigen