Purification, preliminary characterization and in vitro immunomodulatory activity of tiger lily polysaccharide.
A water-soluble polysaccharide (LLPS) from tiger lily was extracted by ultrasonic wave-assisted extraction. The LLPS, which was isolated by alcohol precipitation, was further purified by DEAE Sepharose Fast Flow and Sephadex G-100 chromatography, which resulted in LLPS fractions in LLPS-1, LLPS-2 and LLPS-3, with molecular weights of 350.5, 403.3 and 146.2kDa, respectively. LLPS-1 and LLPS-2 primarily consisted of glucose and mannose in a molar ratio of nearly 1:2 and 1:1, respectively. In contrast, LLPS-3 was primarily composed of arabinose, galactose, glucose and mannose in a molar ratio of nearly 2:2:2:1. LLPS fractions could stimulate the proliferation of macrophages. The in vitro immunomodulatory activity of the fractions was evaluated. The results showed that treatment with 25-400 μg/mL of LLPS fractions could increase phagocytic activity and nitric oxide production of macrophages in a dose-dependent manner.
College of Food Science & Technology, Nanjing Agricultural University, Nanjing, 210095, PR China. Electronic address: zgchen@njau.edu.cn.
Zhi-Gang
ZG
Chen
College of Food Science & Technology, Nanjing Agricultural University, Nanjing, 210095, PR China. Electronic address: 2013108032@njau.edu.cn.
Dan-Ni
DN
Zhang
College of Food Science & Technology, Nanjing Agricultural University, Nanjing, 210095, PR China. Electronic address: btfood313@gmail.com.
Qu
Q
Zhu
College of Food Science & Technology, Nanjing Agricultural University, Nanjing, 210095, PR China. Electronic address: evelen6612@gmail.com.
Qiu-Huizi
QH
Yang
College of Food Science & Technology, Nanjing Agricultural University, Nanjing, 210095, PR China. Electronic address: hangyongbin@njau.edu.cn.
Yong-Bin
YB
Han
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D011232:Chemical Precipitation; D002845:Chromatography / Q000379:methods; D004305:Dose-Response Relationship, Drug; D000431:Ethanol; D007155:Immunologic Factors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D027762:Lilium / Q000737:chemistry*; D008264:Macrophages / Q000187:drug effects* / Q000502:physiology; D051379:Mice; D008970:Molecular Weight; D009569:Nitric Oxide / Q000096:biosynthesis; D010587:Phagocytosis / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D017550:Spectroscopy, Fourier Transform Infrared
D007155:Immunologic Factors; D010936:Plant Extracts; D011134:Polysaccharides; D009569:Nitric Oxide; D000431:Ethanol
Characterization; Invitro immunomodulatory activity; Polysaccharide; Tiger lily