Over-expression of miR-98 in FFPE tissues might serve as a valuable source for biomarker discovery in breast cancer patients.
The miR-98 is thought to be associated with various cancers. This study was to evaluate the potential predictive value of miR-98 expression in formalin-fixed paraffin-embedded tissue of breast cancer patients. The expression levels of miR-98 were examined in 98 breast cancer patients and 40 cancer-free controls using real-time quantitative PCR. The comparison of miR-98 expression levels between patient and control was performed using the Mann-Whitney test. The miR-98 showed higher expression levels in breast cancer patients compared with cancer free controls (p<0.01). The expression levels of miR-98 were highly correlated with miR24/93/378 in breast cancer patients. The miR-98 exhibited great capability of discriminating between cancer patients and controls by the Receiver-operator characteristic (ROC) curve analysis. The miR-98 was found highly correlated with breast cancer by Univariable logistic regression analysis. These results suggest that over-expression of miR-98 in formalin-fixed paraffin-embedded tissues might serve as a valuable source for biomarker discovery in breast cancer patients.
School of Basic Medical Science, Wuhan University Wuhan 430071, People's Republic of China ; Affiliated People's Hospital of Jiangsu University Zhenjiang, 212002, People's Republic of China.
Zhao-Qun
ZQ
Deng
Affiliated People's Hospital of Jiangsu University Zhenjiang, 212002, People's Republic of China.
Jia-Yu
JY
Yin
Affiliated People's Hospital of Jiangsu University Zhenjiang, 212002, People's Republic of China.
Qin
Q
Tang
Department of Nutrition, School of Public Health, Sun Yat-Sen University People's Republic of China.
Feng-Qiong
FQ
Liu
Affiliated People's Hospital of Jiangsu University Zhenjiang, 212002, People's Republic of China.
Jun
J
Qian
Affiliated People's Hospital of Jiangsu University Zhenjiang, 212002, People's Republic of China.
Jiang
J
Lin
Affiliated People's Hospital of Jiangsu University Zhenjiang, 212002, People's Republic of China.
Rui
R
Shao
Affiliated People's Hospital of Jiangsu University Zhenjiang, 212002, People's Republic of China.
Ming
M
Zhang
School of Basic Medical Science, Wuhan University Wuhan 430071, People's Republic of China.
Li
L
He
D000328:Adult; D000368:Aged; D019540:Area Under Curve; D015415:Biomarkers / Q000032:analysis*; D001943:Breast Neoplasms / Q000378:metabolism*; D005260:Female; D005557:Formaldehyde; D006801:Humans; D035683:MicroRNAs / Q000032:analysis*; D008875:Middle Aged; D016612:Paraffin Embedding; D012372:ROC Curve; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D016707:Tissue Fixation; D015854:Up-Regulation
D015415:Biomarkers; C546084:MIRN98 microRNA, human; D035683:MicroRNAs; D005557:Formaldehyde
Biomarkers; breast cancer; cancer; miR-98; miRNA