Surface modification by grafting of poly(SBMA-co-AEMA)-g-PDA coating and its application in CE.
In this paper, a novel copolymer consisting of sulfobetaine methacrylate (SBMA) and 2-aminoethyl methacrylate (AEMA) named as poly(SBMA-co-AEMA) was synthesized by conventional free-radical polymerization, the poly(SBMA-co-AEMA) zwitterionic copolymer was immobilized onto glass slides surface through polydopamine (PDA)-anchored coating and formed poly(SBMA-co-AEMA)-g-PDA coating. The defined copolymer was characterized by nuclear magnetic resonance hydrogen spectroscopy ((1)H NMR) and gel permeation chromatography. The surface morphology, thickness, and chemical component of poly(SBMA-co-AEMA)-g-PDA coating were studied by atom force microscope, ellipsometry, and X-ray photoelectron spectroscopy, respectively. The hydrophilicity and stability of these coatings were investigated by static water contact angles. And finally, the poly(SBMA-co-AEMA)-g-PDA coating was successfully applied into capillary inner surface for suppression electro-osmotic flow and protein separation by capillary electrophoresis.
a CAS Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and Engineering , University of Science and Technology of China , Hefei 230026 , P.R. China.
Lijuan
L
Chen
Lin
L
Tan
Songtao
S
Liu
Longchao
L
Bai
Yanmei
Y
Wang
D000327:Adsorption; D000818:Animals; D019075:Electrophoresis, Capillary / Q000379:methods*; D007211:Indoles / Q000737:chemistry*; D008689:Methacrylates / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification; D013499:Surface Properties
D007211:Indoles; D008689:Methacrylates; D011108:Polymers; D011506:Proteins; C568283:polydopamine; C521589:sulfobetaine methacrylate polymer; C072703:2-aminoethylmethacrylate
AEMA; SBMA; polydopamine; protein separation