Characterization of an antiproliferative exopolysaccharide (LHEPS-2) from Lactobacillus helveticus MB2-1.
In this study, we reported that three purified exopolysaccharides fractions (LHEPS-1, LHEPS-2 and LHEPS-3) and crude LHEPS from the Lactobacillus helveticus MB2-1 inhibited the proliferation potential of human gastric cancer BGC-823 cells. We found that all the LHEPS fractions and crude LHEPS exerted concentration and time dependent inhibitory activities in vitro on BGC-823 cells. LHEPS-2 exhibited higher antiproliferative activity against BGC-823 cells in vitro than LHEPS-1, LHEPS-3 and crude LHEPS. With the consideration of the outstanding antiproliferative effect of LHEPS-2 on BGC-823 cells, more fine structural characterization of LHEPS-2 was further identified. The structure of LHEPS-2 was elucidated by methylated analysis, GC-MS, (1)H NMR and (13)C NMR spectroscopy, together with two-dimensional (2D) COSY, TOCSY, HSQC, HMBC and NOESY spectroscopy. Results showed that the LHEPS-2 was consisted of the following repeating units:
College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
Wei
W
Li
College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
Juan
J
Ji
College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
Weizhi
W
Tang
College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
Xin
X
Rui
College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
Xiaohong
X
Chen
College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
Mei
M
Jiang
College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China. Electronic address: dongms@njau.edu.cn.
Mingsheng
M
Dong
D000970:Antineoplastic Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D002240:Carbohydrate Sequence; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D006801:Humans; D052198:Lactobacillus helveticus / Q000737:chemistry*; D008969:Molecular Sequence Data; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D013274:Stomach Neoplasms / Q000188:drug therapy
D000970:Antineoplastic Agents; D011135:Polysaccharides, Bacterial
Antiproliferative activity; Exopolysaccharides; Lactobacillus helveticus MB2-1; Nuclear magnetic resonance spectroscopy; Structural characterization