Cytotoxic constituents from Lobaria scrobiculata and a comparison of two bioassays for their evaluation.
Lichens are resilient organisms, known for their unique profiles of secondary metabolites and for exhibiting antioxidative, antibacterial, and cytotoxic effects. Analyzing the cytotoxic potential of Lobaria scrobiculata, a bioassay-guided fractionation strategy yielded seven known metabolites, with two of these compounds, 2 and 3, exhibiting cytotoxicity against HL-60 cells. In order to verify the potential impact of degradation on observed bioactivity, a purity and stability evaluation was conducted. The consistency of results obtained by the water-soluble tetrazolium salt-1 assay and trypan blue cytotoxicity assay was evaluated for selected compounds.
Department of Pharmacognosy, Faculty of Life Sciences, University of Vienna , Althanstraße 14, A-1090 Vienna, Austria.
Andreas
A
Schinkovitz
Aman
A
Kaur
Ernst
E
Urban
Martin
M
Zehl
Gabriela
G
Páchniková
Yuehong
Y
Wang
Nadine
N
Kretschmer
Iva
I
Slaninová
Guido F
GF
Pauli
Scott G
SG
Franzblau
Georg
G
Krupitza
Rudolf
R
Bauer
Brigitte
B
Kopp
D000900:Anti-Bacterial Agents / Q000378:metabolism; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D001572:Benzofurans / Q000737:chemistry / Q000494:pharmacology*; D004354:Drug Screening Assays, Antitumor; D005602:France; D018922:HL-60 Cells; D006801:Humans; D008011:Lichens / Q000737:chemistry*; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D014343:Trypan Blue / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D000972:Antineoplastic Agents, Phytogenic; D001572:Benzofurans; D010636:Phenols; C000589917:m-scrobiculin; C073339:usnic acid; D014343:Trypan Blue