Transglutaminase-catalyzed grafting collagen on chitosan and its characterization.
Collagen grafted chitosan was prepared with microbial transglutaminase (MTGase) as biocatalyst which showed high efficiency, selectivity, mild reaction condition and environmental friendliness. The reaction conditions that influenced the degree of substitution (DS) were optimized, which included the reaction time, the reaction temperature, the mass ratio of collagen to chitosan and the mass ratio of MTGase to chitosan. In this study, the water-solubility collagen-chitosan could serve not only to reduce the loss of moisture but also to absorb the moisture. And the moisture absorption and moisture retention abilities were closely related to the DS values. In addition, in vitro antioxidant activity was evaluated in terms of DS values and concentration. Furthermore, L929 mouse fibroblasts were cultured with collagen-chitosan, and methylthiazol tetrazolium (MTT) assay exhibited that collagen-chitosan with DS of 0.660 displayed pronounced cell viability at 2.5mg/ml. Therefore, the water-soluble collagen-chitosan showed the potentiality to repair skin in cosmetic, biomedical and pharmaceutical fields.
College of Chemical Engineering, Wuhan University of Technology, Wuhan 430070, China. Electronic address: lihongfan2000@hotmail.com.
Lihong
L
Fan
College of Chemical Engineering, Wuhan University of Technology, Wuhan 430070, China.
Huan
H
Wu
College of Chemical Engineering, Wuhan University of Technology, Wuhan 430070, China.
Xiaoyu
X
Zhou
College of Chemical Engineering, Wuhan University of Technology, Wuhan 430070, China.
Min
M
Peng
College of Chemical Engineering, Wuhan University of Technology, Wuhan 430070, China.
Jun
J
Tong
The Third Hospital of Wuhan, Wuhan 430060, China.
Weiguo
W
Xie
The Third Hospital of Wuhan, Wuhan 430060, China.
Shuhua
S
Liu
D000818:Animals; D000975:Antioxidants / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D055162:Biocatalysis; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D003094:Collagen / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D051379:Mice; D013302:Streptomyces / Q000201:enzymology; D011503:Transglutaminases / Q000378:metabolism
D000975:Antioxidants; D003094:Collagen; D048271:Chitosan; D011503:Transglutaminases
Antioxidant activity; Cell viability; Chitosan; Collagen; Microbial transglutaminase