Studying the influence of surface topography on bacterial adhesion using spatially organized microtopographic surface patterns.
The influence of surface topography on bacterial adhesion has been investigated using a range of spatially organized microtopographic surface patterns generated on polydimethylsiloxane (PDMS) and three unrelated bacterial strains. The results presented indicate that bacterial cells actively choose their position to settle, differentiating upper and lower areas in all the surface patterns evaluated. Such selective adhesion depends on the cells' size and shape relative to the dimensions of the surface topographical features and surface hydrophobicity/hydrophilicity. Moreover, it was found that all the topographies investigated provoke a significant reduction in bacterial adhesion (30-45%) relative to the smooth control samples regardless of surface hydrophobicity/hydrophilicity. This remarkable finding constitutes a general phenomenon, occurring in both Gram-positive and Gram-negative cells with spherical or rod shape, dictated by only surface topography. Collectively, the results presented in this study demonstrate that spatially organized microtopographic surface patterns represent a promising approach to controlling/inhibiting bacterial adhesion and biofilm formation.
Department of Applied Physics, ‡Department of Biomedical Sciences and §Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), University of Extremadura , Avda de Elvas s/n, 06006 Badajoz, Spain.
David
D
Perera-Costa
José Morales
JM
Bruque
María Luisa
ML
González-Martín
Antonio Cándido
AC
Gómez-García
Virginia
V
Vadillo-Rodríguez
D001422:Bacterial Adhesion / Q000502:physiology*; D006090:Gram-Negative Bacteria / Q000502:physiology; D006094:Gram-Positive Bacteria / Q000502:physiology; D057927:Hydrophobic and Hydrophilic Interactions; D013499:Surface Properties