Comparing the roles of sperm chromatin integrity and apoptosis in intrauterine insemination outcomes of couples with mild male and female factor infertility.
BACKGROUND
Intrauterine insemination (IUI) is one of the therapeutic approaches for infertility. The objective of this study was to evaluate DNA integrity and apoptosis role in success of IUI in both mild male and female factor infertility.


METHODS
PATIENTS WERE DIVIDED INTO TWO GROUPS: M (mild male factor; n = 29) and F (female factor; n = 31) undergoing single IUI. Ejaculates were analyzed and chromatin quality was assessed using chromomycin A3 (CMA3) staining. In addition, spermatozoal apoptosis was recognized using TUNEL assay. Statistical analyses were done using t-test and Mann Whitney test for sperm apoptosis and sperm chromatin by SPSS. Data were expressed in mean±SD for variables. P < 0.05 was considered statistically significant.


RESULTS
Sperm concentration and progressive motility were higher in F than M group. Sperm with normal morphology were statistically similar in M and F infertile patients (32.7±15.6% vs. 35.5±9.07%, p = 0.39). Sperm chromatin immaturity was higher in patients with mild male infertility, when compared with the other group (p < 0.01). Also, 32.0±5.6% and 30.8±6.1% of the spermatozoa showed signs of apoptosis in groups M and F, respectively (p = 0.49). Very low (3.4%) clinical pregnancy rates were noticed in patients with mild male factor infertility.


CONCLUSIONS
Defect in sperm motility as well as high rates of DNA damage and apoptosis may be involved with very low rate of pregnancy outcomes in patients with mild male factor infertility. Therefore, it seems the application of IUI may have better outcomes in patients with female infertility compared to mild male factor infertility.
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Mohammad Ali
MA
Khalili
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Saeedeh
S
Nazari
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Razieh
R
Dehghani-Firouzabadi
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Alireza
A
Talebi
Infertility Center, Isfahan University of Medical Sciences, Isfahan, Iran.
Shekofeh
S
Baghazadeh-Naeini
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Fatemeh
F
Sadeghian-Nodoshan
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Azam
A
Agha-Rahimi
Apoptosis; Infertility; Intrauterine transfusion; Male; Morphology; Sperm motility; Spermatozoa