A novel urea-linked dipodal naphthalene-based fluorescent sensor for Hg(II) and its application in live cell imaging.
A novel cell-permeable urea-linked dipodal naphthalene-based fluorescent receptor 1 (1,1'-(1,5,5-trimethyl-3-oxocyclohexane-1,2-diyl)bis(3-(naphthalen-2-yl)urea) was designed and synthesized. The cation recognition ability of 1 was evaluated with a library of metal ions in DMSO/H₂O (9:1, v/v). The receptor showed a selective chromogenic and fluorescent turn-off response towards Hg(2+) among the surveyed metal ions. The developed sensor was successfully applied to image intracellular Hg(2+) in living cells and also for the determination of Hg(2+) content in real water samples. Moreover, the DFT calculations were performed to complement the experimental evidences.
School of Chemical Sciences, North Maharashtra University, Jalgaon-425001, Maharashtra, India; School of Environmental and Earth Sciences, North Maharashtra University, Jalgaon-425001, Maharashtra, India.
Kundan
K
Tayade
Molecular Biology and Human Genetics Laboratory, Department of Zoology, The University of Burdwan, Burdwan, West Bengal, India.
Banashree
B
Bondhopadhyay
Molecular Biology and Human Genetics Laboratory, Department of Zoology, The University of Burdwan, Burdwan, West Bengal, India.
Anupam
A
Basu
School of Chemical Sciences, Swami Ramanand Teerth Marathawada University, Nanded, Maharashtra, India.
G Krishna
GK
Chaitanya
Department of Applied Chemistry, SV National Institute Technology, Surat-395007, Gujrat, India.
Suban K
SK
Sahoo
Department of Chemistry, Indian Institute Technology, Ropar, Rupnagar, Punjab, India.
Narinder
N
Singh
School of Environmental and Earth Sciences, North Maharashtra University, Jalgaon-425001, Maharashtra, India.
Sanjay
S
Attarde
School of Chemical Sciences, North Maharashtra University, Jalgaon-425001, Maharashtra, India. Electronic address: kuwaras@gmail.com.
Anil
A
Kuwar
D005456:Fluorescent Dyes / Q000737:chemistry* / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D007425:Intracellular Membranes / Q000737:chemistry* / Q000378:metabolism; D008628:Mercury / Q000032:analysis* / Q000378:metabolism; D009281:Naphthalenes / Q000737:chemistry* / Q000378:metabolism; D014508:Urea / Q000737:chemistry* / Q000378:metabolism
D005456:Fluorescent Dyes; D009281:Naphthalenes; C031721:naphthalene; D014508:Urea; D008628:Mercury
Cellular imaging; DFT; Fluorescent sensor; Hg(2+); Real sample analysis; Turn-off