Streptomyces catbensis sp. nov., isolated from soil.
Strain VN07A0015(T) was isolated from soil collected on Cat Ba Island, Vietnam. The taxonomic position of strain VN07A0015(T) was near Streptomyces aomiensis M24DS4(T) (98.5% 16S rRNA gene sequence similarity) and Streptomyces scabrisporus NBRC 100760(T) (95.6%), and it clustered within them; however, this cluster was distant from the type strains of other species of the genus Streptomyces. The aerial mycelia of strain VN07A0015(T) were greyish and formed imperfect spiral spore chains (retinaculiaperti type) with smooth-surfaced spores. The morphological features of strain VN07A0015(T) were different from those of the type strains of S. aomiensis and S. scabrisporus. The chemotaxonomic characteristics of strain VN07A0015(T) were typical for all members of the genus Streptomyces, which possessed ll-type diaminopimelic acid, menaquinone MK-9(H6, H8) and the major fatty acids iso-C(16 : 0) and iso-C(15 : 0). DNA-DNA relatedness between strain VN07A0015(T) and S. aomiensis NBRC 106164(T) was less than 30%. In addition, some physiological and biochemical traits differed from those of S. aomiensis. Therefore, we propose that strain VN07A0015(T) be classified in the genus Streptomyces as a representative of Streptomyces catbensis sp. nov. (type strain VN07A0015(T) = VTCC-A-1889(T) = NBRC 107860(T)).
NITE-Biological Resource Center, National Institute of Technology and Evaluation (NBRC-NITE), Chiba, Japan.
Yayoi
Y
Sakiyama
Institute of Microbiology and Biotechnology, Vietnam National University, Hanoi, Vietnam (IMBT-VNUH).
Nguyen M
NM
Giang
NITE-Biological Resource Center, National Institute of Technology and Evaluation (NBRC-NITE), Chiba, Japan.
Shinji
S
Miyadoh
Institute of Microbiology and Biotechnology, Vietnam National University, Hanoi, Vietnam (IMBT-VNUH).
Dao Thi
DT
Luong
Institute of Microbiology and Biotechnology, Vietnam National University, Hanoi, Vietnam (IMBT-VNUH).
Duong
D
Van Hop
NITE-Biological Resource Center, National Institute of Technology and Evaluation (NBRC-NITE), Chiba, Japan.
Katsuhiko
K
Ando
D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013302:Streptomyces / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D014744:Vietnam; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C073355:menaquinone 9; D003960:Diaminopimelic Acid