L-arabonate and D-galactonate production by expressing a versatile sugar dehydrogenase in metabolically engineered Escherichia coli.
The production of L-arabonate and D-galactonate employing a versatile l-arabinose dehydrogenase (AraDH) from Azospirillum brasilense is presented. The promiscuity of AraDH is manifested by its appreciable activity towards L-arabinose and D-galactose as substrates, and NAD(+) and NADP(+) as cofactors. The AraDH was introduced into an engineered Escherichia coli with inactive L-arabinose or D-galactose metabolism, resulting in strains EMA2 and EWG4, respectively. EMA2 produced 43.9 g L(-1)L-arabonate with a productivity of 1.22 g L(-1)h(-1) and 99.1% (mol/mol) yield. After methanol precipitation, 92.6% of L-arabonate potassium salt was recovered with a purity of 88.8%. Meanwhile, EWG4 produced 24.0 g L(-1)D-galactonate, which is 36% higher than that of the strain carrying the specific d-galactose dehydrogenase. Overall results reveal that the versatility of AraDH to efficiently catalyze the formation of L-arabonate and D-galactonate could be a useful tool in advancing industrial viability for sugar acids production.
Energy and Environment Fusion Technology Center (E(2)FTC), Department of Energy and Biotechnology (DEB), Myongji University, Yongin-si, Gyeonggi-do 449-728, Republic of Korea.
Huaiwei
H
Liu
Energy and Environment Fusion Technology Center (E(2)FTC), Department of Energy and Biotechnology (DEB), Myongji University, Yongin-si, Gyeonggi-do 449-728, Republic of Korea.
Kris Niño G
KN
Valdehuesa
Energy and Environment Fusion Technology Center (E(2)FTC), Department of Energy and Biotechnology (DEB), Myongji University, Yongin-si, Gyeonggi-do 449-728, Republic of Korea.
Kristine Rose M
KR
Ramos
Energy and Environment Fusion Technology Center (E(2)FTC), Department of Energy and Biotechnology (DEB), Myongji University, Yongin-si, Gyeonggi-do 449-728, Republic of Korea.
Grace M
GM
Nisola
Division of Bioscience and Bioinformatics, Myongji University, Yongin-si, Gyeonggi-do 449-728, Republic of Korea.
Won-Keun
WK
Lee
Energy and Environment Fusion Technology Center (E(2)FTC), Department of Energy and Biotechnology (DEB), Myongji University, Yongin-si, Gyeonggi-do 449-728, Republic of Korea. Electronic address: wjc0828@gmail.com.
Wook-Jin
WJ
Chung
D019342:Acetic Acid / Q000378:metabolism; D001089:Arabinose / Q000494:pharmacology; D015806:Azospirillum brasilense / Q000201:enzymology*; D018533:Biomass; D002237:Carbohydrate Dehydrogenases / Q000378:metabolism*; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism*; D005690:Galactose / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism; D060847:Metabolic Engineering / Q000379:methods*; D013400:Sugar Acids / Q000737:chemistry / Q000378:metabolism*
D013400:Sugar Acids; C012991:galactonic acid; D001089:Arabinose; D002237:Carbohydrate Dehydrogenases; C033519:L-arabinose dehydrogenase; D005947:Glucose; D019342:Acetic Acid; D005690:Galactose
E. coli; Metabolic engineering; d-galactonate; l-arabinose dehydrogenase; l-arabonate