Polydopamine-functionalization of graphene oxide to enable dual signal amplification for sensitive surface plasmon resonance imaging detection of biomarker.
Surface plasmon resonance imaging (SPRi) is one of the powerful tools for immunoassays with advantages of label-free, real-time, and high-throughput; however, it often suffers from limited sensitivity. Herein we report a dual signal amplification strategy utilizing polydopamine (PDA) functionalization of reduced graphene oxide (PDA-rGO) nanosheets for sensitive SPRi immunoassay in serum. The PDA-rGO nanosheet is synthesized by oxidative polymerization of dopamine in a gentle alkaline solution in the presence of graphene oxide (GO) sheets and then is antibody-conjugated via a spontaneous reaction between the protein and the PDA component. In the dual amplification mode, the first signal comes from capture of the antibody-conjugated PDA-rGO to form sandwiched immunocomplexes on the SPRi chip, followed by a PDA-induced spontaneous gold reductive deposition on PDA-rGO to further enhance the SPRi signal. The detection limit as low as 500 pg mL(-1) is achieved on a nonfouling SPRi chip with high specificity and a wide dynamic range for a model biomarker, carcinoembryonic antigen (CEA) in 10% human serum.
Institute for Clean Energy & Advanced Materials, Southwest University , Chongqing 400715, China.
Weihua
W
Hu
Guangli
G
He
Huanhuan
H
Zhang
Xiaoshuai
X
Wu
Jialin
J
Li
Zhiliang
Z
Zhao
Yan
Y
Qiao
Zhisong
Z
Lu
Yang
Y
Liu
Chang Ming
CM
Li
D015415:Biomarkers / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D007211:Indoles / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D010087:Oxides / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D020349:Surface Plasmon Resonance
D015415:Biomarkers; D007211:Indoles; D010087:Oxides; D011108:Polymers; C568283:polydopamine; D006108:Graphite