Duffy antigen receptor for chemokine (DARC) polymorphisms and its involvement in acquisition of inhibitory anti-duffy binding protein II (DBPII) immunity.
The Plasmodium vivax Duffy binding protein (PvDBP) and its erythrocytic receptor, the Duffy antigen receptor for chemokines (DARC), are involved in the major P. vivax erythrocyte invasion pathway. An open cohort study to analyze DARC genotypes and their relationship to PvDBP immune responses was carried out in 620 volunteers in an agricultural settlement of the Brazilian Amazon. Three cross-sectional surveys were conducted at 6-month intervals, comprising 395, 410, and 407 subjects, respectively. The incidence rates of P. vivax infection was 2.32 malaria episodes per 100 person-months under survey (95% confidence interval [CI] of 1.92-2.80/100 person-month) and, of P. falciparum, 0.04 per 100 person-months (95% CI of 0.007-0.14/100 person-month). The distribution of DARC genotypes was consistent with the heterogeneous ethnic origins of the Amazon population, with a predominance of non-silent DARC alleles: FY*A > FY*B. The 12-month follow-up study demonstrated no association between DARC genotypes and total IgG antibodies as measured by ELISA targeting PvDBP (region II, DBPII or regions II-IV, DBPII-IV). The naturally acquired DBPII specific binding inhibitory antibodies (BIAbs) tended to be more frequent in heterozygous individuals carrying a DARC-silent allele (FY*BES). These results provide evidence that DARC polymorphisms may influence the naturally acquired inhibitory anti-Duffy binding protein II immunity.
Laboratório de Malária, Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz (FIOCRUZ), Belo Horizonte, MG, Brasil.
Flávia A
FA
Souza-Silva
Laboratório de Malária, Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz (FIOCRUZ), Belo Horizonte, MG, Brasil.
Letícia M
LM
Torres
Laboratório de Malária, Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz (FIOCRUZ), Belo Horizonte, MG, Brasil.
Jessica R
JR
Santos-Alves
Laboratório de Malária, Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz (FIOCRUZ), Belo Horizonte, MG, Brasil.
Michaelis Loren
ML
Tang
Universidade Federal de Mato Grosso, campus Sinop, Sinop, MT, Brasil.
Bruno A M
BA
Sanchez
Laboratório de Malária, Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz (FIOCRUZ), Belo Horizonte, MG, Brasil.
Tais N
TN
Sousa
Julio Müller University Hospital, Universidade Federal de Mato Grosso, Cuiabá, MT, Brasil.
Cor J F
CJ
Fontes
Centro de Pesquisas Leônidas & Maria Deane, FIOCRUZ Amazônia, Manaus, AM, Brasil.
Paulo A
PA
Nogueira
Laboratório de Malária, Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz (FIOCRUZ), Belo Horizonte, MG, Brasil.
Roberto S
RS
Rocha
Laboratório de Malária, Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz (FIOCRUZ), Belo Horizonte, MG, Brasil.
Cristiana F A
CF
Brito
Department of Global Health, College of Public Health, University of South Florida, Tampa, Florida, United States of America.
John H
JH
Adams
Laboratório de Malária, Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz (FIOCRUZ), Belo Horizonte, MG, Brasil.
Flora S
FS
Kano
Laboratório de Malária, Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz (FIOCRUZ), Belo Horizonte, MG, Brasil.
Luzia H
LH
Carvalho
D000293:Adolescent; D000328:Adult; D000483:Alleles; D000953:Antigens, Protozoan / Q000276:immunology*; D003430:Cross-Sectional Studies; D004375:Duffy Blood-Group System / Q000235:genetics*; D005500:Follow-Up Studies; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D016780:Malaria, Vivax / Q000235:genetics / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D015800:Protozoan Proteins / Q000276:immunology*; D011956:Receptors, Cell Surface / Q000235:genetics* / Q000276:immunology*; D055815:Young Adult
C473745:ACKR1 protein, human; D000953:Antigens, Protozoan; D004375:Duffy Blood-Group System; C066001:Duffy antigen binding protein, Plasmodium; D015800:Protozoan Proteins; D011956:Receptors, Cell Surface