Simvastatin coating of TiO₂ scaffold induces osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells.
Bone tissue engineering requires an osteoconductive scaffold, multipotent cells with regenerative capacity and bioactive molecules. In this study we investigated the osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells (hAD-MSCs) on titanium dioxide (TiO2) scaffold coated with alginate hydrogel containing various concentrations of simvastatin (SIM). The mRNA expression of osteoblast-related genes such as collagen type I alpha 1 (COL1A1), alkaline phosphatase (ALPL), osteopontin (SPP1), osteocalcin (BGLAP) and vascular endothelial growth factor A (VEGFA) was enhanced in hAD-MSCs cultured on scaffolds with SIM in comparison to scaffolds without SIM. Furthermore, the secretion of osteoprotegerin (OPG), vascular endothelial growth factor A (VEGFA), osteopontin (OPN) and osteocalcin (OC) to the cell culture medium was higher from hAD-MSCs cultured on scaffolds with SIM compared to scaffolds without SIM. The TiO2 scaffold coated with alginate hydrogel containing SIM promote osteogenic differentiation of hAD-MSCs in vitro, and demonstrate feasibility as scaffold for hAD-MSC based bone tissue engineering.
Department of Biomaterials, Institute of Clinical Dentistry, University of Oslo, Norway.
Helen
H
Pullisaar
Department of Biomaterials, Institute of Clinical Dentistry, University of Oslo, Norway.
Janne E
JE
Reseland
Department of Biomaterials, Institute of Clinical Dentistry, University of Oslo, Norway.
Håvard J
HJ
Haugen
Institute of Immunology, Oslo University Hospital Rikshospitalet, Norway; Norwegian Center for Stem Cell Research, Department of Biochemistry, Institute of Basic Medical Sciences, University of Oslo, Norway.
Jan E
JE
Brinchmann
Department of Biomaterials, Institute of Clinical Dentistry, University of Oslo, Norway; Norwegian Center for Stem Cell Research, Department of Biochemistry, Institute of Basic Medical Sciences, University of Oslo, Norway. Electronic address: esben.ostrup@odont.uio.no.
Esben
E
Ostrup
D000273:Adipose Tissue / Q000166:cytology*; D000328:Adult; D002454:Cell Differentiation / Q000187:drug effects*; D002470:Cell Survival; D000090602:Collagen Type I, alpha 1 Chain; D005260:Female; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000187:drug effects / Q000502:physiology*; D008875:Middle Aged; D015675:Osteocalcin / Q000378:metabolism; D010012:Osteogenesis; D053495:Osteopontin / Q000378:metabolism; D053244:Osteoprotegerin / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D019821:Simvastatin / Q000494:pharmacology*; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds; D014025:Titanium; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
C000716127:COL1A1 protein, human; D000090602:Collagen Type I, alpha 1 Chain; D053244:Osteoprotegerin; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; D015675:Osteocalcin; D053495:Osteopontin; C009495:titanium dioxide; D019821:Simvastatin; D014025:Titanium
Alginate hydrogel; Human adipose tissue-derived mesenchymal stem cells; Simvastatin; TiO(2) scaffold