Highly efficient multiplex targeted mutagenesis and genomic structure variation in Bombyx mori cells using CRISPR/Cas9.
Bombyx mori is an economically important insect and a model organism for studying lepidopteran and arthropod biology. Using a highly efficient CRISPR/Cas9 system, we showed that this system could mediate highly efficient targeted genome editing of a single gene locus, large chromosomal deletion or inversion, and also multiplex genome editing of 6 genes simultaneously in BmNs cell line derived from B. mori. The simplicity and high efficiency of our system provide unprecedented possibilities for researchers to implement precise and sophisticated manipulation of a chosen B. mori gene in BmNs cells easily in a limited time course, and perhaps new opportunities for functional genomics of B. mori and other lepidopteran insects.
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, PR China.
Yuanyuan
Y
Liu
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, PR China.
Sanyuan
S
Ma
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, PR China.
Xiaogang
X
Wang
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, PR China.
Jiasong
J
Chang
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, PR China.
Jie
J
Gao
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, PR China.
Run
R
Shi
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, PR China.
Jianduo
J
Zhang
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, PR China.
Wei
W
Lu
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, PR China.
Yue
Y
Liu
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, PR China.
Ping
P
Zhao
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, PR China. Electronic address: xiaqy@swu.edu.cn.
Qingyou
Q
Xia
D000818:Animals; D012831:Bombyx / Q000235:genetics*; D064112:Clustered Regularly Interspaced Short Palindromic Repeats; D018390:Gene Targeting / Q000379:methods*; D014644:Genetic Variation; D023281:Genomics; D019476:Insect Proteins / Q000235:genetics; D016296:Mutagenesis
D019476:Insect Proteins
Bombyx mori; CRISPR/Cas9; Chromosomal deletion; Chromosomal inversion; Multiplex genome editing; Mutagenesis