Comprehensive analysis of vascular endothelial growth factor-C related factors in stomach cancer.
BACKGROUND
Vascular endothelial growth factor-C (VEGF-C), which contributes to lymphatic metastasis (LM) in malignant disease, is one of the most important factors involved in physical and pathological lymphangiogenesis. Some VEGF-C related factors such as sine oculis homeobox homolog (SIX) 1, contactin (CNTN) 1 and dual specificity phosphatase (DUSP) 6 have been extensively studied in malignancies, but their expression levels and associations have still to be elucidated in stomach cancer.


METHODS
We detected their expression levels in 30 paired stomach cancer tissues using quantitative real-time reverse transcription-PCR (qRT-PCR). The expression and clinical significance of each factor was analyzed using Wilcoxon signed rank sum test. The correlation among all the factors was performed by Spearman rank correlation analysis.


RESULTS
The results suggest that VEGF-C and CNTN1 are significantly correlated with tumor size, SIX1 with the age and CNTN1 also with the cTNM stage. There are significant correlations of expression levels among VEGF-C, SIX1, CNTN1 and DUSP6.


CONCLUSIONS
There exists an important regulatory crosstalk involving SIX1, VEGF-C, CNTN1 and DUSP6 in stomach cancer.
Department of General Surgery, Huashan Hospital, Fudan University, Shanghai, China E-mail : guangjian_huang@163.com, zhangwenhong@fudan.edu.cn.
Yong-Chao
YC
Liu
Jing
J
Zhao
Cheng-En
CE
Hu
Jun
J
Gan
Wen-Hong
WH
Zhang
Guang-Jian
GJ
Huang
D000328:Adult; D000368:Aged; D058967:Contactin 1 / Q000235:genetics; D054642:Dual Specificity Phosphatase 6 / Q000235:genetics; D005260:Female; D018398:Homeodomain Proteins / Q000235:genetics; D006801:Humans; D008297:Male; D008875:Middle Aged; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology*; D042582:Vascular Endothelial Growth Factor C / Q000235:genetics*
C548669:CNTN1 protein, human; D058967:Contactin 1; D018398:Homeodomain Proteins; C099236:SIX1 protein, human; D042582:Vascular Endothelial Growth Factor C; C517035:DUSP6 protein, human; D054642:Dual Specificity Phosphatase 6