Cepharanthine inhibited HIV-1 cell-cell transmission and cell-free infection via modification of cell membrane fluidity.
The anti-HIV-1 activity of cepharanthine (CEP), a natural product derived from Stephania cepharantha Hayata, was evaluated. CEP stabilized plasma membrane fluidity and inhibited HIV-1 envelope-dependent cell-to-cell fusion of HIV-1-infected cells as well as cell-free infection. It is suggested that CEP inhibited the HIV-1 entry process by reducing plasma membrane fluidity, and the plasma membrane is therefore an identical target to prevent viral infection.
Division of Hematopoiesis, Center for AIDS Research, Kumamoto University, 2-2-1 Honjo, Chuo-ku, Kumamoto 860-0811, Japan.
Kouki
K
Matsuda
Division of Hematopoiesis, Center for AIDS Research, Kumamoto University, 2-2-1 Honjo, Chuo-ku, Kumamoto 860-0811, Japan.
Shinichiro
S
Hattori
Division of Applied Life Science, Graduate School of Engineering, Sojo University, 4-22-1 Ikeda, Kumamoto 860-0082, Japan.
Yuji
Y
Komizu
Division of Hematopoiesis, Center for AIDS Research, Kumamoto University, 2-2-1 Honjo, Chuo-ku, Kumamoto 860-0811, Japan.
Ryusho
R
Kariya
Division of Applied Life Science, Graduate School of Engineering, Sojo University, 4-22-1 Ikeda, Kumamoto 860-0082, Japan.
Ryuichi
R
Ueoka
Division of Hematopoiesis, Center for AIDS Research, Kumamoto University, 2-2-1 Honjo, Chuo-ku, Kumamoto 860-0811, Japan. Electronic address: okadas@kumamoto-u.ac.jp.
Seiji
S
Okada
D044182:Benzylisoquinolines / Q000302:isolation & purification / Q000494:pharmacology*; D002478:Cells, Cultured; D057809:HEK293 Cells; D023581:HIV Fusion Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D015658:HIV Infections / Q000517:prevention & control* / Q000635:transmission* / Q000821:virology; D015497:HIV-1 / Q000187:drug effects* / Q000502:physiology; D054884:Host-Pathogen Interactions / Q000187:drug effects*; D006801:Humans; D019169:Jurkat Cells; D008214:Lymphocytes / Q000821:virology; D008560:Membrane Fluidity / Q000187:drug effects*; D031607:Stephania / Q000737:chemistry; D053586:Virus Internalization / Q000187:drug effects; D052117:Benzodioxoles
D044182:Benzylisoquinolines; D023581:HIV Fusion Inhibitors; C006947:cepharanthine; D052117:Benzodioxoles
Cell fusion; Cepharanthine; HIV-1; Membrane fluidity; Viral infection