Physical, structural, antioxidant and antimicrobial properties of gelatin-chitosan composite edible films.
Physico-chemical and mechanical properties of cuttlefish skin gelatin (G), chitosan (C) from shrimp (Penaeus kerathurus) and composite films (G75/C25, G50/C50, G25/C75) plasticized with glycerol were investigated. The results indicated that chitosan film had higher tensile strength and lower elongation at break when compared with the other films. Composite films show no significant difference in tensile strength (TS), thickness and transparency. The structural properties evaluated by FTIR and DSC showed total miscibility between both polymers. DSC scans showed that the increase of chitosan content in the composite films increases the transition temperature (Tg) and enthalpy (ΔHg) of films. The morphology study of gelatin, chitosan and composite films showed a compact and homogenous structure. In addition, gelatin and G75/C25 films demonstrated a high antioxidant activities monitored by β-carotene bleaching, DPPH radical-scavenging and reducing power activities, while films contained chitosan exhibited higher antimicrobial activity against Gram-positive than Gram-negative bacteria.
Laboratoire de Génie Enzymatique et de Microbiologie - Université de Sfax, Ecole Nationale d'Ingénieurs de Sfax, B.P. 1173, 3038 Sfax, Tunisia. Electronic address: jridimourad@gmail.com.
Mourad
M
Jridi
Laboratoire de Génie Enzymatique et de Microbiologie - Université de Sfax, Ecole Nationale d'Ingénieurs de Sfax, B.P. 1173, 3038 Sfax, Tunisia.
Sawssan
S
Hajji
Laboratoire de Génie Enzymatique et de Microbiologie - Université de Sfax, Ecole Nationale d'Ingénieurs de Sfax, B.P. 1173, 3038 Sfax, Tunisia.
Hanen Ben
HB
Ayed
Laboratoire de Génie Enzymatique et de Microbiologie - Université de Sfax, Ecole Nationale d'Ingénieurs de Sfax, B.P. 1173, 3038 Sfax, Tunisia.
Imen
I
Lassoued
Laboratoire de Chimie Industrielle, Université de Sfax, Ecole Nationale d'Ingénieurs de Sfax, B.P. 1173, 3038 Sfax, Tunisia.
Aïcha
A
Mbarek
Laboratoire de Génie Enzymatique et de Microbiologie - Université de Sfax, Ecole Nationale d'Ingénieurs de Sfax, B.P. 1173, 3038 Sfax, Tunisia.
Maher
M
Kammoun
Laboratoire de Biodiversité et Biotechnologie Marine, Institut National des Sciences et Technologies de la Mer, Centre de Sfax, B.P. 1035, 3018 Sfax, Tunisia.
Nabil
N
Souissi
Laboratoire de Génie Enzymatique et de Microbiologie - Université de Sfax, Ecole Nationale d'Ingénieurs de Sfax, B.P. 1173, 3038 Sfax, Tunisia.
Moncef
M
Nasri
D000818:Animals; D000890:Anti-Infective Agents / Q000008:administration & dosage / Q000737:chemistry*; D000975:Antioxidants / Q000008:administration & dosage / Q000737:chemistry*; D048271:Chitosan / Q000737:chemistry*; D049832:Decapodiformes / Q000737:chemistry; D005780:Gelatin / Q000737:chemistry* / Q000648:ultrastructure; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D010539:Permeability / Q000187:drug effects; D012995:Solubility
D000890:Anti-Infective Agents; D000975:Antioxidants; C015233:gelatin film; D005780:Gelatin; D048271:Chitosan
Antioxidant and antibacterial activity; Composite gelatin–chitosan films; Microstructure