Matrix metalloproteinases 2 and 9 and e-cadherin expression in the endometrium during the implantation window of infertile women before in vitro fertilization treatment.
OBJECTIVE
To evaluate the expression of endometrial matrix metalloproteinases (MMPs) 2 and 9 and E-cadherin in peri-implantation phase of infertile women who have undergone in vitro fertilization (IVF) cycles.


METHODS
This prospective study included 51 patients who underwent endometrial biopsy during the receptive phase in a menstrual cycle prior to IVF treatment. The samples were evaluated by tissue microarray for immunohistochemical study.


RESULTS
The expression of MMP-2, MMP-9, and E-cadherin in the endometrium prior to IVF treatment was not associated with pregnancy. There was a decrease in E-cadherin immunodetection, the higher the age of the patients, a negative relationship between E-cadherin and MMP-2, and a positive association between MMP-9 and E-cadherin.


CONCLUSIONS
The MMP-2, MMP-9, and E-cadherin are expressed in the endometrium of infertile patients during the receptive phase of the natural menstrual cycle. However, there is no correlation between the expression of these molecules and the clinical IVF outcomes.
Gynecology Department of Paulista School of Medicine, Federal University of Sao Paulo (EPM-UNIFESP), Sao Paulo, Brazil Centre of Human Reproduction, Hospital e Maternidade Santa Joana, São Paulo, Brazil vambertof@gmail.com.
Vamberto O A
VO
Maia-Filho
Huntington Reproductive Medicine, Av República do Líbano, São Paulo, Brazil.
Andre M
AM
Rocha
Gynecology Department of Paulista School of Medicine, Federal University of Sao Paulo (EPM-UNIFESP), Sao Paulo, Brazil Centre of Human Reproduction, Hospital e Maternidade Santa Joana, São Paulo, Brazil.
Fernando P
FP
Ferreira
Gynecology Department of Paulista School of Medicine, Federal University of Sao Paulo (EPM-UNIFESP), Sao Paulo, Brazil.
Tatiana C S
TC
Bonetti
Centre of Human Reproduction, Hospital e Maternidade Santa Joana, São Paulo, Brazil Huntington Reproductive Medicine, Av República do Líbano, São Paulo, Brazil Gynecology Discipline of School of Medicine, University of Sao Paulo (USP), Sao Paulo, Brazil.
Paulo
P
Serafini
Gynecology Department of Paulista School of Medicine, Federal University of Sao Paulo (EPM-UNIFESP), Sao Paulo, Brazil Centre of Human Reproduction, Hospital e Maternidade Santa Joana, São Paulo, Brazil Huntington Reproductive Medicine, Av República do Líbano, São Paulo, Brazil.
Eduardo L A
EL
Motta
D000328:Adult; D015703:Antigens, CD; D001706:Biopsy; D015820:Cadherins / Q000032:analysis*; D010064:Embryo Implantation; D004717:Endometrium / Q000201:enzymology* / Q000503:physiopathology; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D007150:Immunohistochemistry; D007247:Infertility, Female / Q000201:enzymology* / Q000503:physiopathology / Q000628:therapy; D020778:Matrix Metalloproteinase 2 / Q000032:analysis*; D020780:Matrix Metalloproteinase 9 / Q000032:analysis*; D008597:Menstrual Cycle / Q000378:metabolism; D011247:Pregnancy; D011446:Prospective Studies; D046888:Tissue Array Analysis; D016896:Treatment Outcome
D015703:Antigens, CD; C513384:CDH1 protein, human; D015820:Cadherins; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9
E-cadherin; IVF; endometrium; metalloproteinase 2; metalloproteinase 9