A competitive electrochemical immunosensor for the detection of human interleukin-6 based on the electrically heated carbon electrode and silver nanoparticles functionalized labels.
A facile one-step electrochemical reduction method was developed to prepare electrochemically reduced graphene oxide (ERGO) and gold-palladium bimetallic nanoparticles (AuPdNPs) as the platform of immunosensor. A novel competitive electrochemical immunosensor was then proposed by combining the ERGO-AuPdNPs platform with silver nanoparticles (AgNPs) functionalized polystyrene bionanolabel for the sensitive detection of human interleukin-6 (IL-6). An electrically heated carbon electrode (HCPE) was introduced in the detection procedure of the immunosensor, and further improved the sensitivity. The immunosensor exhibited a wide linear response to IL-6 ranging from 0.1 to 100000 pg mL(-1) with a detection limit of 0.059 pg mL(-1). The proposed method showed good precision, broad linear range, acceptable stability and high reproducibility, and could be used for the detection of IL-6 in real samples, which possessed promising application in clinical research.
School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, P. R. China. Electronic address: lou@seu.edu.cn.
Yongbing
Y
Lou
State Key Lab of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210093, P. R. China; School of Chemical Engineering, Anhui University of Science and Technology, Huainan, 232001, China.
Tingting
T
He
State Key Lab of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210093, P. R. China.
Fang
F
Jiang
State Key Lab of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210093, P. R. China; School of Chemical Engineering, Anhui University of Science and Technology, Huainan, 232001, China.
Jian-Jun
JJ
Shi
State Key Lab of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210093, P. R. China. Electronic address: jjzhu@nju.edu.cn.
Jun-Jie
JJ
Zhu
D015374:Biosensing Techniques / Q000379:methods; D055664:Electrochemical Techniques / Q000379:methods; D004566:Electrodes; D006358:Hot Temperature; D006801:Humans; D007118:Immunoassay; D015850:Interleukin-6 / Q000032:analysis*; D053768:Metal Nanoparticles / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*
D015850:Interleukin-6; D012834:Silver
AgNPs; ERGO−AuPdNPs; HCPE; IL-6; Immunosensor