Identification of L-amino acid oxidase (Mb-LAAO) with antibacterial activity in the venom of Montivipera bornmuelleri, a viper from Lebanon.
The L-amino acid oxidase (LAAO) is a multifunctional enzyme, able to partake in different activities including antibacterial activity. In this study, a novel LAAO (Mb-LAAO) was isolated from the venom of M. bornmuelleri snake using size exclusion chromatography followed by RP-HPLC and partially characterized. However, the molecular weight of the Mb-LAAO determined by ESI-MS and SDS-PAGE was 59 960.4 Da. Once the enzymatic activity test confirming the enzyme's identity (transformation of L-leucine) was done, the Mb-LAAO was evaluated for its antibacterial activity against Gram-negative bacteria. It showed a remarkable effect against M. morganii and K. pneumoniae. Moreover, no cytotoxic activity was observed for Mb-LAAO against human erythrocytes arguing for an exploration of its pharmaceutical interest.
Mohamad
M
Rima
Claudine
C
Accary
Katia
K
Haddad
Riyad
R
Sadek
Souad
S
Hraoui-Bloquet
Jean C
JC
Desfontis
Azm Center for Research in Biotechnology and its Applications, Doctoral School of Science and Technology, Lebanese University, El Mittein Street, Tripoli, Lebanon. zfajloun@ul.edu.lb.
Ziad
Z
Fajloun
D000818:Animals; D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006801:Humans; D050830:L-Amino Acid Oxidase / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D007861:Lebanon; D008970:Molecular Weight; D021241:Spectrometry, Mass, Electrospray Ionization; D014757:Viper Venoms / Q000201:enzymology*; D017819:Viperidae
D000900:Anti-Bacterial Agents; D014757:Viper Venoms; D050830:L-Amino Acid Oxidase