Squamous cell carcinoma arising in association with verruca vulgares and HPV-2: a clinicopathologic study with p16 and p53 immunohistochemical studies and human papillomavirus in situ hybridization studies.
BACKGROUND
We observed cutaneous squamous cell carcinomas of the skin (SCCS) with histologic features suggesting they are arising in association with verruca vulgares (SCC-VV). We analyzed SCC-VV to determine what types of human papillomaviruses (HPVs) could be detected by in situ hybridization. We also analyzed demographic and clinical features and performed immunohistochemical studies for p53, p16, and Ki-67.


METHODS
Five cases of SCC-VV were chosen and compared with 5 cases each of cutaneous squamous cell carcinoma with bowenoid features (SCC-BD), and cutaneous squamous cell carcinoma with keratoacanthomatous features (SCC-KA).


RESULTS
We identified HPV-2 in 3 of 5 SCC-VV and SCC-KA, and 2 of 5 SCC-BD. SCC-VV showed a decreased amount of p16 immunoexpression compared with the other types, particularly SCC-BD (P=0.0108 ). SCC-KA showed a decreased p53 (P=0.0096) and Ki-67 (P=0.0007) immunoexpression compared with the other types.


CONCLUSIONS
SCC-VV seems to occur in older immunocompetent individuals and may be distinguishable from other SCCS types by histologic and immunohistochemical methods. HPV-2 and HPV-1 were the only types of HPV identified in this study, including SCCS with such diverse morphologic appearances as SCC-VV, SCC-BD, and SCC-KA. Further studies are needed to confirm these findings.
*Department of Pathology, Virginia Mason Medical Center, Seattle, WA †Comprehensive Cancer Center, Ohio State University, Columbus, OH.
Steven M
SM
Ruhoy
Donald G
DG
Guinee
Gerard
G
Nuovo
D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000150:complications / Q000175:diagnosis* / Q000235:genetics / Q000821:virology; D019941:Cyclin-Dependent Kinase Inhibitor p16; D005260:Female; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D019394:Ki-67 Antigen / Q000235:genetics; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000235:genetics; D027383:Papillomaviridae / Q000502:physiology; D030361:Papillomavirus Infections / Q000150:complications / Q000175:diagnosis* / Q000235:genetics / Q000821:virology; D012878:Skin Neoplasms / Q000150:complications / Q000175:diagnosis* / Q000235:genetics / Q000821:virology; D016159:Tumor Suppressor Protein p53 / Q000235:genetics; D014860:Warts / Q000150:complications / Q000175:diagnosis* / Q000235:genetics / Q000821:virology
D014408:Biomarkers, Tumor; C000614131:CDKN2A protein, human; D019941:Cyclin-Dependent Kinase Inhibitor p16; D019394:Ki-67 Antigen; D009363:Neoplasm Proteins; D016159:Tumor Suppressor Protein p53