High genetic diversity of Anaplasma marginale detected from Philippine cattle.
A total of 658 cattle in 6 provinces in the Philippines were screened for Anaplasma marginale infection by using a diagnostic heat-shock operon (groEL) gene-PCR assay. The screening-positive samples were further tested using the major surface antigen protein 1a (Msp1a) gene-PCR assay. Screening PCR results showed 130 cattle (19.8%) were positive for the A. marginale infection. Subsequent amplification using the Msp1a gene only showed 93 samples (14.1%) to be positive. In addition, 37 tandem-repeat structures, including 20 novel structures, and 41 distinct genotypes were identified. Interestingly, multiple infections of 4 different genotypes were also observed in A. marginale-infected cattle. The present study demonstrated the prevalence and characterization of diverse genotypes of A. marginale in the Philippine cattle.
Department of Clinical Veterinary Sciences, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan.
Adrian Patalinghug
AP
Ybañez
Rochelle Haidee D
RH
Ybañez
Florencia G
FG
Claveria
Mary Jane
MJ
Cruz-Flores
Xuen
X
Xuenan
Naoaki
N
Yokoyama
Hisashi
H
Inokuma
D042322:Anaplasma marginale / Q000235:genetics*; D000712:Anaplasmosis / Q000453:epidemiology / Q000469:parasitology*; D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology*; D014644:Genetic Variation; D010679:Philippines / Q000453:epidemiology