Physiological hepatic response to zinc oxide nanoparticle exposure in the white sucker, Catostomus commersonii.
Liver toxicity of commercially relevant zinc oxide nanoparticles (nZnO) was assessed in a benthic freshwater cypriniform, the white sucker (Catostomus commersonii). Exposure to nZnO caused several changes in levels of liver enzyme activity, antioxidants, and lipid peroxidation end products consistent with an oxidative stress response. Aconitase activity decreased by ~65% but tended to be restored to original levels upon supplementation with Fe(2+), indicating oxidative inactivation of the 4Fe-4S cluster. Furthermore, glucose-6-phosphate dehydrogenase activity decreased by ~29%, and glutathione levels increased by ~56%. Taken together, these suggest that nZnO induces hepatic physiological stress. Each assay was then validated by using a single liver homogenate or plasma sample that was partitioned and treated with nZnO or Zn(2+), the breakdown product of nZnO. It was found that Zn(2+), but not nZnO, increased detected glutathione reductase activity by ~14% and decreased detected malondialdehyde by ~39%. This indicates that if appreciable nZnO dissolution occurs in liver samples during processing and assay, it may skew results, with implications not only for this study, but also for a wide range of nanotoxicology studies focusing on nZnO. Finally, in vitro incubations of cell-free rat blood plasma with nZnO failed to generate any significant increase in malondialdehyde or protein carbonyl levels, or any significant decrease in ferric reducing ability of plasma. This suggests that at the level tested, any oxidative stress caused by nZnO is the result of a coordinated physiological response by the liver.
Department of Chemistry and Biochemistry, Mount Allison University, Barclay Chemistry Building, 63C York Street, Sackville, New Brunswick E4L 1G8, Canada. Electronic address: cdieni@mta.ca.
Christopher Anthony
CA
Dieni
Department of Chemistry and Biochemistry, Mount Allison University, Barclay Chemistry Building, 63C York Street, Sackville, New Brunswick E4L 1G8, Canada.
Neal Ingraham
NI
Callaghan
Department of Chemistry and Biochemistry, Mount Allison University, Barclay Chemistry Building, 63C York Street, Sackville, New Brunswick E4L 1G8, Canada.
Patrick Thomas
PT
Gormley
Department of Chemistry and Biochemistry, Mount Allison University, Barclay Chemistry Building, 63C York Street, Sackville, New Brunswick E4L 1G8, Canada.
Kathryn Marie Alison
KM
Butler
Department of Chemistry and Biochemistry, Mount Allison University, Barclay Chemistry Building, 63C York Street, Sackville, New Brunswick E4L 1G8, Canada.
Tyson James
TJ
Maccormack
D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D056486:Chemical and Drug Induced Liver Injury / Q000201:enzymology / Q000473:pathology*; D003531:Cypriniformes / Q000502:physiology*; D005260:Female; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D053758:Nanoparticles / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects; D050050:Protein Carbonylation / Q000187:drug effects; D011506:Proteins / Q000378:metabolism; D051381:Rats; D013473:Sunscreening Agents / Q000633:toxicity*; D015034:Zinc Oxide / Q000633:toxicity*
D000975:Antioxidants; D011506:Proteins; D013473:Sunscreening Agents; D008315:Malondialdehyde; D015034:Zinc Oxide
Assay validation; Fish; Liver response; Nanotoxicity; Oxidative stress; Zinc oxide nanoparticles