Structural basis for hijacking siderophore receptors by antimicrobial lasso peptides.
The lasso peptide microcin J25 is known to hijack the siderophore receptor FhuA for initiating internalization. Here, we provide what is to our knowledge the first structural evidence on the recognition mechanism, and our biochemical data show that another closely related lasso peptide cannot interact with FhuA. Our work provides an explanation on the narrow activity spectrum of lasso peptides and opens the path to the development of new antibacterials.
1] Department of Life Sciences, Imperial College London, London, UK. [2] Membrane Protein Lab, Diamond Light Source, Harwell Science and Innovation Campus, Chilton, Oxfordshire, UK. [3] Rutherford Appleton Laboratory, Research Complex at Harwell, Didcot, Oxfordshire, UK.
Indran
I
Mathavan
Communication Molecules and Adaptation of Microorganisms Laboratory, UMR 7245 CNRS-Muséum National d'Histoire Naturelle, Paris, France.
Séverine
S
Zirah
Department of Chemistry, University of Oxford, Oxford, UK.
Shahid
S
Mehmood
1] Department of Life Sciences, Imperial College London, London, UK. [2] Membrane Protein Lab, Diamond Light Source, Harwell Science and Innovation Campus, Chilton, Oxfordshire, UK. [3] Rutherford Appleton Laboratory, Research Complex at Harwell, Didcot, Oxfordshire, UK.
Hassanul G
HG
Choudhury
Communication Molecules and Adaptation of Microorganisms Laboratory, UMR 7245 CNRS-Muséum National d'Histoire Naturelle, Paris, France.
Christophe
C
Goulard
Communication Molecules and Adaptation of Microorganisms Laboratory, UMR 7245 CNRS-Muséum National d'Histoire Naturelle, Paris, France.
Yanyan
Y
Li
Department of Chemistry, University of Oxford, Oxford, UK.
Carol V
CV
Robinson
Communication Molecules and Adaptation of Microorganisms Laboratory, UMR 7245 CNRS-Muséum National d'Histoire Naturelle, Paris, France.
Sylvie
S
Rebuffat
1] Department of Life Sciences, Imperial College London, London, UK. [2] Membrane Protein Lab, Diamond Light Source, Harwell Science and Innovation Campus, Chilton, Oxfordshire, UK. [3] Rutherford Appleton Laboratory, Research Complex at Harwell, Didcot, Oxfordshire, UK.
Konstantinos
0000000157274721
K
Beis
D000890:Anti-Infective Agents / Q000378:metabolism* / Q000494:pharmacology; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D001430:Bacteriocins / Q000378:metabolism*; D004705:Endocytosis; D004926:Escherichia coli / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000378:metabolism; D008958:Models, Molecular; D011487:Protein Conformation; D011956:Receptors, Cell Surface / Q000378:metabolism*
D000890:Anti-Infective Agents; D001425:Bacterial Outer Membrane Proteins; D001430:Bacteriocins; D029968:Escherichia coli Proteins; C050900:FhuA protein, E coli; D011956:Receptors, Cell Surface; C035902:siderophore receptors; C020411:microcin