Functional modification of Drosophila intestinal stem cells by ionizing radiation.
Although the diverse effects of ionizing radiation on biological and pathological processes at various levels ranging from molecular to whole body are well studied, the effects on adult stem cells by ionizing radiation remain largely unknown. In this study, we characterized the functional modifications of adult Drosophila midgut intestinal stem cells after ionizing radiation treatment. A dose of 10 Gy of radiation decreased the proliferative capacity of intestinal stem cells. Interestingly, after irradiation at 2 Gy, the intestinal stem cells exhibited increased proliferative activity, misdifferentiation and γH2AvD and 8-oxo-dG levels. In addition, the guts irradiated with 2 Gy showed increased JNK and AKT activities. Furthermore, we showed that 2 Gy of ionizing radiation induced centrosome amplification in intestinal stem cells of adult midguts. Our data gives molecular insights into the effects of ionizing radiation on functional modifications of stem cells. The adult Drosophila midgut intestinal stem cells offer a potentially rich new system for the exploration of the biological effects of ionizing radiation.
a  Department of Molecular Biology, Pusan National University, Busan 609-735, Korea;
Jung-Hoon
JH
Pyo
Joung-Sun
JS
Park
Hyun-Jin
HJ
Na
Ho-Jun
HJ
Jeon
Shin-Hae
SH
Lee
Joong-Gook
JG
Kim
So-Young
SY
Park
Young-Woo
YW
Jin
Young-Shin
YS
Kim
Mi-Ae
MA
Yoo
D000818:Animals; D049109:Cell Proliferation / Q000528:radiation effects; D018385:Centrosome; D004249:DNA Damage; D004330:Drosophila; D007422:Intestines / Q000166:cytology / Q000528:radiation effects*; D011839:Radiation, Ionizing; D013234:Stem Cells / Q000166:cytology / Q000528:radiation effects*