Gene silencing in crustaceans: from basic research to biotechnologies.
Gene silencing through RNA interference (RNAi) is gaining momentum for crustaceans, both in basic research and for commercial development. RNAi has proven instrumental in a growing number of crustacean species, revealing the functionality of novel crustacean genes essential among others to development, growth, metabolism and reproduction. Extensive studies have also been done on silencing of viral transcripts in crustaceans, contributing to the understanding of the defense mechanisms of crustaceans and strategies employed by viruses to overcome these. The first practical use of gene silencing in aquaculture industry has been recently achieved, through manipulation of a crustacean insulin-like androgenic gland hormone. This review summarizes the advancements in the use of RNAi in crustaceans, and assesses the advantages of this method, as well as the current hurdles that hinder its large-scale practice.
Department of Life Sciences and the National Institute for Biotechnology in the Negev, Ben-Gurion University, P.O. Box 653, Beer Sheva 84105, Israel. sagia@bgu.ac.il.
Amir
A
Sagi
Department of Life Sciences and the National Institute for Biotechnology in the Negev, Ben-Gurion University, P.O. Box 653, Beer Sheva 84105, Israel. mnor@bgu.ac.il.
Rivka
R
Manor
Faculty of Science, Health, Education and Engineering, GeneCology Research Centre, University of the Sunshine Coast, 4 Locked Bag, Maroochydore, Queensland 4558, Australia. tventura@usc.edu.au.
Tomer
T
Ventura