Tetraspanins CD9 and CD151 at the immune synapse support T-cell integrin signaling.
Understanding how the immune response is activated and amplified requires detailed knowledge of the stages in the formation of the immunological synapse (IS) between T lymphocytes and antigen-presenting cells (APCs). We show that tetraspanins CD9 and CD151 congregate at the T-cell side of the IS. Silencing of CD9 or CD151 blunts the IL-2 secretion and expression of the activation marker CD69 by APC-conjugated T lymphocytes, but does not affect the accumulation of CD3 or actin to the IS, or the translocation of the microtubule-organizing center toward the T-B contact area. CD9 or CD151 silencing diminishes the relocalization of α4β1 integrin to the IS and reduces the accumulation of high-affinity β1 integrins at the cell-cell contact. These changes are accompanied by diminished phosphorylation of the integrin downstream targets FAK and ERK1/2. Our results suggest that CD9 and CD151 support integrin-mediated signaling at the IS.
Servicio de Inmunología, Hospital de la Princesa, Instituto de Investigación Sanitaria La Princesa, Madrid, Spain; Vascular Biology and Inflammation Department, Centro Nacional de Investigaciones Cardiovasculares (CNIC), Madrid, Spain.
Vera
V
Rocha-Perugini
José Maria
JM
González-Granado
Emilio
E
Tejera
Soraya
S
López-Martín
Maria
M
Yañez-Mó
Francisco
F
Sánchez-Madrid
D006801:Humans; D054992:Immunological Synapses / Q000276:immunology*; D016023:Integrins / Q000502:physiology*; D019169:Jurkat Cells; D008213:Lymphocyte Activation; D015398:Signal Transduction / Q000502:physiology*; D013601:T-Lymphocytes / Q000276:immunology*; D060185:Tetraspanin 24 / Q000502:physiology*; D060245:Tetraspanin 29 / Q000502:physiology*
C554715:CD151 protein, human; C554869:CD9 protein, human; D016023:Integrins; D060185:Tetraspanin 24; D060245:Tetraspanin 29
CD151; CD9; Immune synapse; Integrins; T-cell activation