Animal models in dengue.
Validation of a mouse model of dengue virus (DENV) infection relies on verification of viremia and productive replication in mouse tissues following infection. Here, we describe a quantitative assay for determining viral RNA levels in mouse serum and tissues. For the purpose of confirming DENV replication, we outline a fluorescence immunohistochemistry (FIHC) protocol for staining a nonstructural protein of DENV.
Division of Vaccine Discovery, La Jolla Institute for Allergy and Immunology, 9420 Athena Circle, La Jolla, CA, 92037, USA.
Emily
E
Plummer
Sujan
S
Shresta
D000818:Animals; D003715:Dengue / Q000821:virology*; D003716:Dengue Virus / Q000235:genetics; D004195:Disease Models, Animal; D005453:Fluorescence; D007150:Immunohistochemistry; D051379:Mice; D012313:RNA / Q000302:isolation & purification; D020365:RNA Helicases / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012697:Serine Endopeptidases / Q000378:metabolism; D013154:Spleen / Q000821:virology; D017361:Viral Nonstructural Proteins / Q000378:metabolism; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases
D012313:RNA; D020365:RNA Helicases; D012697:Serine Endopeptidases; D017361:Viral Nonstructural Proteins; C060028:NS3 protein, flavivirus; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases