Evaluation of efficacy of natural astaxanthin and vitamin E in prevention of colistin-induced nephrotoxicity in the rat model.
OBJECTIVE
We evaluated the effect of astaxanthin (ASX) and vitamin E (vit E) on colistin methanesulfonate (CMS) induced-nephrotoxicity in rats.


METHODS
Animals were treated with sterile saline, 300000 or 450 000 IU/kg/day of CMS, CMS + ASX (20 mg/kg), CMS + vit E (100 mg/kg), or CMS + 1 ml/kg olive oil (OO) for 7 days. The plasma/urine creatinine (Cr) level, urine γ-glutamyl-transferase (GGT) level, and renal tissue activities in malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and reductase (GSH), as well as renal histology were performed.


RESULTS
CMS induced a tubular damage, increased the GGT and MDA levels, and decreased the activities of SOD, CAT, GPx and GSH. Co-treatment with ASX or vit E restored all biochemical parameters cited above and improved the histopathological damage.


CONCLUSIONS
Nephrotoxicity induced by CMS might be due to oxidative damage. The improvement by ASX or vit E seems to be related to their antioxidant properties.
Research unit of pharmacology and toxicology of xenobiotics (UR12 ES13), Faculty of Medicine, University of Sfax, 3029, Tunisia. Electronic address: ghlissi_zohra@yahoo.fr.
Zohra
Z
Ghlissi
Research unit of pharmacology and toxicology of xenobiotics (UR12 ES13), Faculty of Medicine, University of Sfax, 3029, Tunisia.
Ahmed
A
Hakim
Unit of enzyme and Bioconversion, National School of Engineering, University of Sfax, Tunisia.
Assaad
A
Sila
Anatomopathology Laboratory, CHU Habib Bourguiba, 3029 Sfax, Tunisia.
Hela
H
Mnif
Research unit of pharmacology and toxicology of xenobiotics (UR12 ES13), Faculty of Medicine, University of Sfax, 3029, Tunisia.
Khaled
K
Zeghal
Laboratory of Histology and Embryology, Faculty of Medicine, University of Sfax, Tunisia.
Tarek
T
Rebai
Unit of enzyme and Bioconversion, National School of Engineering, University of Sfax, Tunisia.
Ali
A
Bougatef
Research unit of pharmacology and toxicology of xenobiotics (UR12 ES13), Faculty of Medicine, University of Sfax, 3029, Tunisia.
Zouheir
Z
Sahnoun
D000818:Animals; D000900:Anti-Bacterial Agents; D000975:Antioxidants / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D003091:Colistin; D003404:Creatinine / Q000097:blood; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D007668:Kidney / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D007674:Kidney Diseases / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D014810:Vitamin E / Q000494:pharmacology*; D024341:Xanthophylls / Q000494:pharmacology; D005723:gamma-Glutamyltransferase / Q000652:urine
D000900:Anti-Bacterial Agents; D000975:Antioxidants; D024341:Xanthophylls; D014810:Vitamin E; D008315:Malondialdehyde; C005948:astaxanthine; D003404:Creatinine; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D005723:gamma-Glutamyltransferase; D003091:Colistin
Astaxanthin; Colistin; Nephrotoxicity; Oxidative damage; Vitamin E