The phosphoproteome in regenerating protoplasts from Physcomitrella patens protonemata shows changes paralleling postembryonic development in higher plants.
The moss Physcomitrella patens is an ideal model plant to study plant developmental processes. To better understand the mechanism of protoplast regeneration, a phosphoproteome analysis was performed. Protoplasts were prepared from protonemata. By 4 d of protoplast regeneration, the first cell divisions had ensued. Through a highly selective titanium dioxide (TiO2)-based phosphopeptide enrichment method and mass spectrometric technology, more than 300 phosphoproteins were identified as protoplast regeneration responsive. Of these, 108 phosphoproteins were present on day 4 but not in fresh protoplasts or those cultured for 2 d. These proteins are catalogued here. They were involved in cell-wall metabolism, transcription, signal transduction, cell growth/division, and cell structure. These protein functions are related to cell morphogenesis, organogenesis, and development adjustment. This study presents a comprehensive analysis of phosphoproteome involved in protoplast regeneration and indicates that the mechanism of plant protoplast regeneration is similar to that of postembryonic development.
Key Laboratory of Urban Agriculture (North) Ministry of Agriculture, Beijing University of Agriculture, Beijing 102206, China.
Xiaoqin
X
Wang
Meiyan
M
Qi
Jingyun
J
Li
Zhongzhong
Z
Ji
Yong
Y
Hu
Fang
F
Bao
Ramamurthy
R
Mahalingam
Yikun
Y
He
D019068:Bryopsida / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D002853:Chromatography, Liquid; D010750:Phosphoproteins / Q000235:genetics* / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D020543:Proteome / Q000235:genetics* / Q000378:metabolism; D011523:Protoplasts / Q000166:cytology / Q000502:physiology*; D012038:Regeneration; D053719:Tandem Mass Spectrometry
D010750:Phosphoproteins; D010940:Plant Proteins; D020543:Proteome
LC-MS/MS; Physcomitrella patens; TiO2 enrichment.; phosphoproteome; postembryonic development; protoplast regeneration