Effect of low concentration sodium dodecyl sulfate on the electromigration of palonosetron hydrochloride stereoisomers in micellar electrokinetic chromatography.
The effect of low concentrations of sodium dodecyl sulfate (SDS) on the separation of palonosetron hydrochloride (PALO) stereoisomers by micellar electrokinetic chromatography (MEKC) has been investigated. It was found that the addition of SDS prolongs the migration time and the migration order of four stereoisomers changes regularly with the SDS concentration. Good separations for all the four stereoisomers were achieved at appropriate SDS concentration. The effect of SDS on the electromigration (mobilities) of PALO stereoisomers has been studied, in order to explain its effect on the separation by MEKC. It was found that low concentrations of SDS added into the separation media forms negatively charged complexes with PALO stereoisomers and hence reverses their electromigration direction. Furthermore, the migration order between two enantiomeric pairs is also reversed because the enantiomeric pair with a bigger positive mobility than that of another pair turns to have a bigger negative mobility when bound with SDS. Based on these results, the effect of SDS on the MEKC separation of PALO stereoisomers was elucidated reasonably. The performance of the developed chiral MEKC method was validated by the analysis of a real sample.
College of Chemistry and Chemical Engineering, Luoyang Normal University, Luoyang 471022, China. Electronic address: shaoqianghu@sina.com.
Shao-Qiang
SQ
Hu
College of Chemistry and Chemical Engineering, Luoyang Normal University, Luoyang 471022, China.
Gui-Xia
GX
Wang
College of Chemistry and Chemical Engineering, Luoyang Normal University, Luoyang 471022, China.
Wen-Bo
WB
Guo
School of Chemical Engineering & Pharmaceutics, Henan University of Science and Technology, Luoyang 471003, China.
Xu-Ming
XM
Guo
College of Chemistry and Molecular Science, Wuhan University, Wuhan 430072, China.
Min
M
Zhao
D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods; D007546:Isoquinolines / Q000737:chemistry*; D008823:Micelles; D000077924:Palonosetron; D011812:Quinuclidines / Q000737:chemistry*; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry*; D013237:Stereoisomerism
D007546:Isoquinolines; D008823:Micelles; D011812:Quinuclidines; D012967:Sodium Dodecyl Sulfate; D000077924:Palonosetron
Chiral separation; Micellar electrokinetic chromatography; Migration order; Palonosetron hydrochloride; Sodium cholate; Sodium dodecyl sulfate