Light-sheet microscopy using an Airy beam.
Light-sheet microscopy facilitates rapid, high-contrast, volumetric imaging with minimal sample exposure. However, the rapid divergence of a traditional Gaussian light sheet restricts the field of view (FOV) that provides innate subcellular resolution. We show that the Airy beam innately yields high contrast and resolution up to a tenfold larger FOV. In contrast to the Bessel beam, which also provides an increased FOV, the Airy beam's characteristic asymmetric excitation pattern results in all fluorescence contributing positively to the contrast, enabling a step change for light-sheet microscopy.
School of Physics and Astronomy, University of St Andrews, St Andrews, UK.
Tom
T
Vettenburg
School of Physics and Astronomy, University of St Andrews, St Andrews, UK.
Heather I C
HI
Dalgarno
1] School of Physics and Astronomy, University of St Andrews, St Andrews, UK. [2] School of Biology, University of St Andrews, St Andrews, UK.
Jonathan
J
Nylk
The Scottish Oceans Institute, Gatty Marine Laboratory, School of Biology, University of St Andrews, St Andrews, UK.
Clara
C
Coll-Lladó
The Scottish Oceans Institute, Gatty Marine Laboratory, School of Biology, University of St Andrews, St Andrews, UK.
David E K
DE
Ferrier
School of Medicine, University of St Andrews, St Andrews, UK.
Tomáš
T
Čižmár
School of Biology, University of St Andrews, St Andrews, UK.
Frank J
FJ
Gunn-Moore
School of Physics and Astronomy, University of St Andrews, St Andrews, UK.
Kishan
K
Dholakia
D000818:Animals; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism; D003198:Computer Simulation; D004867:Equipment Design; D005456:Fluorescent Dyes / Q000737:chemistry; D008027:Light; D008853:Microscopy / Q000295:instrumentation* / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods; D008863:Microspheres; D016011:Normal Distribution; D055095:Optics and Photonics; D012542:Scattering, Radiation; D015027:Zebrafish
D005456:Fluorescent Dyes