A novel Omp25-binding peptide screened by phage display can inhibit Brucella abortus 2308 infection in vitro and in vivo.
Brucellosis is a globally distributed zoonotic disease affecting animals and humans, and current antibiotic and vaccine strategies are not optimal. The surface-exposed protein Omp25 is involved in Brucella virulence and plays an important role in Brucella pathogenesis during infection, suggesting that Omp25 could be a useful target for selecting potential therapeutic molecules to inhibit Brucella pathogenesis. In this study, we identified, we believe for the first time, peptides that bind specifically to the Omp25 protein of pathogens, using a phage panning technique, After four rounds of panning, 42 plaques of eluted phages were subjected to pyrosequencing. Four phage clones that bound better than the other clones were selected following confirmation by ELISA and affinity constant determination. The peptides selected could significantly inhibit Brucella abortus 2308 (S2308) internalization and intracellular growth in RAW264.7 macrophages, and significantly induce secretion of TNF-α and IL-12 in peptide- and S2308-treated cells. Any observed peptide (OP11, OP27, OP35 or OP40) could significantly inhibit S2308 infection in BALB/c mice. Moreover, the peptide OP11 was the best candidate peptide for inhibiting S2308 infection in vitro and in vivo. These results suggest that peptide OP11 has potential for exploitation as a peptide drug in resisting S2308 infection.
College of Animal Science and Technology, Shihezi University, Shihezi 832003, PR China.
Junbo
J
Zhang
College of Medicine, Shihezi University, Shihezi 832003, PR China.
Fei
F
Guo
College of Life Sciences, Shihezi University, Xinjiang 832000, PR China.
Xiaoqiang
X
Huang
College of Animal Science and Technology, Shihezi University, Shihezi 832003, PR China.
Chuangfu
C
Chen
College of Life Sciences, Tsinghua University, Beijing 100083, PR China.
Ruitian
R
Liu
College of Animal Science and Technology, Shihezi University, Shihezi 832003, PR China.
Hui
H
Zhang
College of Medicine, Shihezi University, Shihezi 832003, PR China.
Yuanzhi
Y
Wang
College of Medicine, Shihezi University, Shihezi 832003, PR China.
Shuanghong
S
Yin
College of Animal Science and Technology, Shihezi University, Shihezi 832003, PR China.
Zhiqiang
Z
Li
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002003:Brucella abortus / Q000378:metabolism* / Q000472:pathogenicity*; D002006:Brucellosis / Q000188:drug therapy*; D002460:Cell Line; D005260:Female; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry* / Q000627:therapeutic use*; D011485:Protein Binding; D014774:Virulence
D001426:Bacterial Proteins; D019151:Peptide Library; D010455:Peptides