High-resolution melting assay for genotyping of IFNL4-associated dinucleotide variant rs368234815.
Interferon-λ4-related dinucleotide variant rs368234815 TT/-G is strongly linked with rs12979860 polymorphism, the most important genetic marker connected to treatment-induced hepatitis C virus clearance. Due to additional baseline information that rs368234815 polymorphism could provide for the management of chronic hepatitis C, we developed and validated a high-resolution melting genotyping assay using 193 patients with chronic hepatitis C.
Division of Gastroenterology and Hepatology, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Università degli Studi di Milano, Milan, Italy.
E
E
Galmozzi
F
F
Facchetti
R
R
Perbellini
A
A
Aghemo
D000998:Antiviral Agents / Q000627:therapeutic use*; D015331:Cohort Studies; D005838:Genotype; D060005:Genotyping Techniques / Q000379:methods*; D019698:Hepatitis C, Chronic / Q000188:drug therapy* / Q000276:immunology*; D006801:Humans; D007378:Interleukins / Q000235:genetics*; D016896:Treatment Outcome; D000095022:Interferon Lambda
D000998:Antiviral Agents; D007378:Interleukins; C579163:IFNL4 protein, human; D000095022:Interferon Lambda
IFNL4; IL28B; RT-PCR; hepatitis C virus; high-resolution melting