Quantitative phosphoproteomics analysis of nitric oxide-responsive phosphoproteins in cotton leaf.
Knowledge of phosphorylation events and their regulation is crucial to understanding the functional biology of plant proteins, but very little is currently known about nitric oxide-responsive phosphorylation in plants. Here, we report the first large-scale, quantitative phosphoproteome analysis of cotton (Gossypium hirsutum) treated with sodium nitroprusside (nitric oxide donor) by utilizing the isobaric tag for relative and absolute quantitation (iTRAQ) method. A total of 1315 unique phosphopeptides, spanning 1528 non-redundant phosphorylation sites, were detected from 1020 cotton phosphoproteins. Among them, 183 phosphopeptides corresponding to 167 phosphoproteins were found to be differentially phosphorylated in response to sodium nitroprusside. Several of the phosphorylation sites that we identified, including RQxS, DSxE, TxxxxSP and SPxT, have not, to our knowledge, been reported to be protein kinase sites in other species. The phosphoproteins identified are involved in a wide range of cellular processes, including signal transduction, RNA metabolism, intracellular transport and so on. This study reveals unique features of the cotton phosphoproteome and provides new insight into the biochemical pathways that are regulated by nitric oxide.
State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, Henan Province, China.
Shuli
S
Fan
Key Laboratory of Cotton Biology and Breeding in the Middle Reaches of the Changjing River, Institute of Economic Crop, Hubei Academy of Agricultural Science, Wuhan, Hubei Province, China.
Yanyan
Y
Meng
State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, Henan Province, China.
Meizhen
M
Song
State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, Henan Province, China.
Chaoyou
C
Pang
State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, Henan Province, China.
Hengling
H
Wei
State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, Henan Province, China; College of Agronomy, Northwest A & F University, Yangling, Shaanxi Province, China.
Ji
J
Liu
Key Laboratory of Cotton Biology and Breeding in the Middle Reaches of the Changjing River, Institute of Economic Crop, Hubei Academy of Agricultural Science, Wuhan, Hubei Province, China.
Xianjin
X
Zhan
Key Laboratory of Cotton Biology and Breeding in the Middle Reaches of the Changjing River, Institute of Economic Crop, Hubei Academy of Agricultural Science, Wuhan, Hubei Province, China.
Jiayang
J
Lan
Key Laboratory of Cotton Biology and Breeding in the Middle Reaches of the Changjing River, Institute of Economic Crop, Hubei Academy of Agricultural Science, Wuhan, Hubei Province, China.
Changhui
C
Feng
Key Laboratory of Cotton Biology and Breeding in the Middle Reaches of the Changjing River, Institute of Economic Crop, Hubei Academy of Agricultural Science, Wuhan, Hubei Province, China.
Shengxi
S
Zhang
State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, Henan Province, China.
Shuxun
S
Yu
D020816:Amino Acid Motifs; D003368:Gossypium / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism*; D010748:Phosphopeptides / Q000378:metabolism; D010750:Phosphoproteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D018515:Plant Leaves / Q000378:metabolism*; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics
D010748:Phosphopeptides; D010750:Phosphoproteins; D010940:Plant Proteins; D020543:Proteome; D009569:Nitric Oxide