Caloric restriction prevents lead-induced oxidative stress and inflammation in rat liver.
The aim of the present study is to investigate the effects of caloric restriction on liver of lead-administered rat. Male Sprague-Dawley rats were randomly divided into two groups: Ad libitum fed group (AL, free access to normal rat chow) and caloric restriction group (CR, fed 65% of AL animals' food intake). After 6 weeks, half of the animals of each group were injected lead acetate and the other half were injected saline. Liver tissue samples were collected at the end of the experiments. Glutathione peroxidase (GPx), superoxide dismutase (SOD), malondialdehyde (MDA), and tumor necrosis factor (TNF-α) were measured in the tissue extracts. Histological studies were also performed. Our results showed that lead administrations (not saline injections) reduced liver SOD and GPx and increased MDA and TNF-α in AL animals, but in the CR animals lead injections did not significantly change the measured parameters. The histological studies supported the biochemical findings. We concluded that 65% CR may prevent lead-induced oxidative stress and inflammation in rat liver.
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz 51656-65811, Iran.
Mustafa
M
Mohammadi
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz 51656-65811, Iran.
Rana
R
Ghaznavi
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz 51656-65811, Iran.
Rana
R
Keyhanmanesh
Department of Physiology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran 1417613151, Iran.
Hamid Reza
HR
Sadeghipour
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz 51656-65811, Iran.
Roya
R
Naderi
Dental School, Tabriz University of Medical Sciences, Tabriz 51656-65814, Iran.
Hossein
H
Mohammadi
D000818:Animals; D031204:Caloric Restriction; D007249:Inflammation / Q000517:prevention & control*; D007854:Lead / Q000633:toxicity*; D008099:Liver / Q000187:drug effects* / Q000473:pathology; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley
D007854:Lead