Quantitative analysis of trace Pb(II) by a DNAzyme cracking-rhodamine 6G SERRS probe on Au(core)Ag(shell) nanosol substrate.
In pH 7.2 Tris-HCl buffer solution containing 0.09 mol/L NaCl at 80°C, the single-stranded substrate DNA hybrids with the enzyme DNA to form double-stranded DNA (dsDNA). The substrate chain of dsDNA could be cracked catalytically by Pb(2+) to produce a short single-stranded DNA (ssDNA) that adsorbed on the Au(core)Ag(shell) nanoparticle (Au/AgNP) surface to form stable Au/AgNP-ssDNA conjugate to prevent aggregation by NaCl, and it combined with rhodamine 6G (RhG) to form RhG-Au/AgNP-ssDNA probe that exhibited a strong surface-enhanced resonance Raman scattering (SERRS) peak at 1510 cm(-1). With the increase of Pb(2+) concentration, the SERRS peak increased linearly due to the more RhG-Au/AgNP-ssDNA probe forming. Under the selected conditions, the increased SERRS intensity ΔI was linear to Pb(2+) concentration in the range of 5.0×10(-8)-7.0×10(-7) mol/L, with a detection limit of 7×10(-9) mol/L Pb(2+).
Key Laboratory of Ecology of Rare and Endangered Species and Environmental Conservation of Education Ministry, Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guangxi Normal University, Guilin 541004, China.
Qingye
Q
Liu
Key Laboratory of Ecology of Rare and Endangered Species and Environmental Conservation of Education Ministry, Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guangxi Normal University, Guilin 541004, China.
Yanyan
Y
Wei
Key Laboratory of Ecology of Rare and Endangered Species and Environmental Conservation of Education Ministry, Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guangxi Normal University, Guilin 541004, China.
Yanghe
Y
Luo
Key Laboratory of Ecology of Rare and Endangered Species and Environmental Conservation of Education Ministry, Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guangxi Normal University, Guilin 541004, China.
Aihui
A
Liang
Key Laboratory of Ecology of Rare and Endangered Species and Environmental Conservation of Education Ministry, Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guangxi Normal University, Guilin 541004, China. Electronic address: zljiang@mailbox.gxnu.edu.cn.
Zhiliang
Z
Jiang
D004277:DNA, Single-Stranded / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D007854:Lead / Q000032:analysis*; D053768:Metal Nanoparticles / Q000737:chemistry*; D012235:Rhodamines / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*; D013059:Spectrum Analysis, Raman / Q000379:methods
D004277:DNA, Single-Stranded; D012235:Rhodamines; C026188:rhodamine 6G; D007854:Lead; D012834:Silver; D006046:Gold
Au(core)Ag(shell) nanosol; DNAzyme cracking; Pb(2+); Rhodamine 6G; SERRS