On farm production of arbuscular mycorrhizal fungi inoculum using lignocellulosic agrowastes.
The present study evaluated the efficiency of lignocellulosic agrowastes produced in Brazil as substrates for production of on farm AMF inoculum and tested different diluents and inoculation techniques. In a first experiment, Sorghum bicolor seedlings were colonized with Rhizophagus clarus or Claroideoglomus etunicatus and transplanted to 20 L bags containing sugarcane bagasse (SC), king palm leaf sheets (KP), or barley hulls (BH) mixed (1:1:1 or 2:1:1, v/v/v) with sand and rice shell. SC and KP were conducive for production of spores and infectious propagules. A number of infectious propagules obtained were greater than with BH and ranged from 233-350 propagules cm(-3) for both isolates in SC and KP at the1:1:1 mix dilution. Number of spores of both fungi was affected mainly by the SC agrowaste, and spore densities were significantly higher compared to KP and BH. In a second experiment, SC was mixed with soil or sand and inoculation consisted of transplanting colonized seedlings or adding soil inoculum. Number of propagules tended to differ for each fungus according to the inoculation technique or diluent. It is concluded from the data that SC and KP are suitable agrowastes to be incorporated in substrates for producing AMF inoculum using the on farm method.
Programa de Pós-Graduação em Engenharia Ambiental, Universidade Regional de Blumenau (FURB), Rua São Paulo 3250, 89030-000, Blumenau, SC, Brazil.
Thiago Roberto
TR
Schlemper
Sidney Luiz
SL
Stürmer
D000383:Agriculture / Q000379:methods*; D007220:Industrial Waste; D008031:Lignin / Q000737:chemistry*; D038821:Mycorrhizae / Q000502:physiology*; D018517:Plant Roots / Q000382:microbiology; D012988:Soil Microbiology; D045868:Sorghum / Q000382:microbiology*; D013172:Spores, Fungal / Q000254:growth & development
D007220:Industrial Waste; C036909:lignocellulose; D008031:Lignin