Brazilin ameliorates high glucose-induced vascular inflammation via inhibiting ROS and CAMs production in human umbilical vein endothelial cells.
Vascular inflammatory process has been suggested to play a key role in the initiation and progression of atherosclerosis, a major complication of diabetes mellitus. Recent studies have shown that brazilin exhibits antihepatotoxic, antiplatelet, cancer preventive, or anti-inflammatory properties. Thus, we investigated whether brazilin suppresses vascular inflammatory process induced by high glucose (HG) in cultured human umbilical vein endothelial cells (HUVEC). HG induced nitrite production, lipid peroxidation, and intracellular reactive oxygen species formation in HUVEC cells, which was reversed by brazilin. Western blot analysis revealed that brazilin markedly inhibited HG-induced phosphorylation of endothelial nitric oxide synthase. Besides, we investigated the effects of brazilin on the MAPK signal transduction pathway because MAPK families are associated with vascular inflammation under stress. Brazilin blocked HG-induced phosphorylation of extracellular signal-regulated kinase and transcription factor NF-κB. Furthermore, brazilin concentration-dependently attenuated cell adhesion molecules (ICAM-1 and VCAM-1) expression induced by various concentrations of HG in HUVEC. Taken together, the present data suggested that brazilin could suppress high glucose-induced vascular inflammatory process, which may be closely related with the inhibition of oxidative stress, CAMs expression, and NF-κB activation in HUVEC. Our findings may highlight a new therapeutic intervention for the prevention of vascular diseases.
Department of Pharmacology, College of Medicine, Taipei Medical University, Taipei, Taiwan.
Thanasekaran
T
Jayakumar
Department of Pharmacology, College of Medicine, Taipei Medical University, Taipei, Taiwan ; Department of Cardiology, Cathay General Hospital, Taipei, Taiwan.
Chao-Chien
CC
Chang
Department of Surgery, Taipei Hospital, Ministry of Health and Welfare, New Taipei City, Taiwan.
Shoei-Loong
SL
Lin
School of Oral Hygiene, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan.
Yung-Kai
YK
Huang
Taipei Medical University, Taiwan.
Chien-Ming
CM
Hu
Department of Biotechnology, Periyar Maniammai University, Thanjavur, Tamil Nadu, India.
Antoinet Ramola
AR
Elizebeth
Division of Allergy and Immunology, Department of Internal Medicine, Cathay General Hospital, No. 280, Section 4, Jen-Ai Road, Taipei, Taiwan ; The Laboratory of Allergy and Immunology, Cathay Medical Research Institute, New Taipei City, Taiwan ; Department of Medicine, School of Medicine, Fu Jen Catholic University, New Taipei City, Taiwan.
Shih-Chang
SC
Lin
Emergency Department, Taipei Hospital, Ministry of Health and Welfare, No. 127, Su-Yuan Road, Hsin-Chuang, New Taipei City, Taiwan ; Department of General Medicine, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan.
Cheuk-Sing
CS
Choy
D001578:Benzopyrans / Q000494:pharmacology*; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002478:Cells, Cultured; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005947:Glucose / Q000494:pharmacology*; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D007249:Inflammation / Q000188:drug therapy* / Q000378:metabolism; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D010766:Phosphorylation / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D019010:Vascular Cell Adhesion Molecule-1 / Q000378:metabolism
D001578:Benzopyrans; D015815:Cell Adhesion Molecules; D016328:NF-kappa B; D017382:Reactive Oxygen Species; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1; D052250:Nitric Oxide Synthase Type III; D048049:Extracellular Signal-Regulated MAP Kinases; D020928:Mitogen-Activated Protein Kinases; C044362:brazilin; D005947:Glucose