Improving oral human papillomavirus detection using toothbrush sampling in HIV-positive men who have sex with men.
Pre- and postabrasion oral rinse samples (ORS) and a toothbrush sample detected human papillomavirus (HPV) DNA in at least one sample among 45 (26%) of 173 HIV-positive men who have sex with men. There was moderate agreement for HPV genotype detection between the preabrasion and postabrasion ORS (κ = 0.49; 95% confidence interval [CI], 0.37 to 0.61). There was good agreement between postabrasion ORS and toothbrushes (κ = 0.70; 95% CI, 0.60 to 0.80). The sensitivities for HPV genotypes detected were 80% (95% CI, 69 to 88) for preabrasion ORS, 65% (95% CI, 54 to 76) for postabrasion ORS, and 75% (95% CI, 63 to 84) for toothbrushes.
Melbourne School of Population and Global Health, University of Melbourne, Melbourne, Australia j.ong@unimelb.edu.au.
Jason J
JJ
Ong
Melbourne Sexual Health Centre, Melbourne, Australia Central Clinical School, Monash University, Clayton, Australia.
Tim
T
Read
Melbourne Sexual Health Centre, Melbourne, Australia Central Clinical School, Monash University, Clayton, Australia.
Marcus
M
Chen
Melbourne Sexual Health Centre, Melbourne, Australia.
Sandra
S
Walker
Kirby Institute, University of New South Wales, Sydney, Australia.
Matthew
M
Law
Melbourne Sexual Health Centre, Melbourne, Australia Central Clinical School, Monash University, Clayton, Australia.
Catriona
C
Bradshaw
Department of Obstetrics and Gynaecology, University of Melbourne, Melbourne, Australia Regional HPV Lab Net Reference Laboratory, Department of Microbiology and Infectious Diseases, The Royal Women's Hospital, Melbourne, Australia Murdoch Childrens Research Institute, Parkville, Australia.
Suzanne M
SM
Garland
Department of Obstetrics and Gynaecology, University of Melbourne, Melbourne, Australia Regional HPV Lab Net Reference Laboratory, Department of Microbiology and Infectious Diseases, The Royal Women's Hospital, Melbourne, Australia Murdoch Childrens Research Institute, Parkville, Australia.
Sepehr N
SN
Tabrizi
Regional HPV Lab Net Reference Laboratory, Department of Microbiology and Infectious Diseases, The Royal Women's Hospital, Melbourne, Australia.
Alyssa
A
Cornall
Kirby Institute, University of New South Wales, Sydney, Australia.
Andrew
A
Grulich
Melbourne School of Population and Global Health, University of Melbourne, Melbourne, Australia.
Jane
J
Hocking
Melbourne Sexual Health Centre, Melbourne, Australia Central Clinical School, Monash University, Clayton, Australia.
Christopher K
CK
Fairley
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D003945:Diagnosis, Oral / Q000379:methods*; D015658:HIV Infections / Q000150:complications; D018451:Homosexuality, Male; D006801:Humans; D008297:Male; D008875:Middle Aged; D027383:Papillomaviridae / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000175:diagnosis*; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000379:methods*; D055815:Young Adult