A novel approach for antibody nanocarriers development through hydrophobic ion-pairing complexation.
IgG-Fab fragment, a model antibody protein was hydrophobically modified by a novel approach of ion-pairing complexation. Three different sulphated ion-pairing agents were utilised including sodium dodecyl sulphate, taurocholic acid and dextran sulphate (DS). The formations of hydrophobic ion-pairing (HIP) complexes were dependant on pH and molar ratio of ion-pairing agent to Fab. Aqueous solubilities of HIP complexes were very low compared to Fab alone. In particular, when dextran sulphate was added as ion-pairing agent, formed Fab:DS HIP complexes were least soluble in water. Further, nanoparticles (NPs) loaded with drug and Fab:DS HIP complex were prepared and characterised with respect to encapsulation efficiency and size. We observed significant improvement in encapsulation efficiency for Fab:DS HIP complex-loaded nanoparticles. This study demonstrates a novel approach of formulating antibody-loaded nanoparticles which can also be employed for delivery of large antibodies.
Division of Pharmaceutical Sciences, School of Pharmacy, University of Missouri-Kansas City , Kansas City, MO , USA.
Ashaben
A
Patel
Ripal
R
Gaudana
Ashim K
AK
Mitra
D016264:Dextran Sulfate / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D007140:Immunoglobulin Fab Fragments / Q000737:chemistry*; D007074:Immunoglobulin G / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry*; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D013656:Taurocholic Acid / Q000737:chemistry
D004337:Drug Carriers; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D012967:Sodium Dodecyl Sulfate; D013656:Taurocholic Acid; D016264:Dextran Sulfate
Antibody fragment; IgG-Fab fragment; dextran sulphate; hydrophobic ion-pairing complex; nanoparticles; protein