Effects of light wavelengths on extracellular and capsular polysaccharide production by Nostoc flagelliforme.
The influences of different wavelengths of light (red 660nm, yellow 590nm, green 520nm, blue 460nm, purple 400nm) and white light on extracellular polysaccharide (EPS) and capsular polysaccharide (CPS) production by Nostoc flagelliforme in liquid culture were demonstrated in this study. The results showed that, compared with white light, red and blue lights significantly increased both EPS and CPS production while yellow light reduced their production; purple and green lights stimulated EPS production but inhibited CPS formation. Nine constituent monosaccharides and one uronic acid were detected in both EPS and CPS, and their ratios showed significant differences among treatment with different light wavelengths. However, the advanced structure of EPS and CPS from various light conditions did not present obvious difference through Fourier transform infrared spectroscopy and X-ray diffraction characterization. These findings establish a basis for development of high-yielding polysaccharide production process and understanding their regulation.
Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, School of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, PR China; Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin University, Tianjin 300072, PR China.
Pei-pei
PP
Han
Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, School of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, PR China.
Ying
Y
Sun
Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, School of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, PR China. Electronic address: jiashiru@tust.edu.cn.
Shi-ru
SR
Jia
Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, School of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, PR China.
Cheng
C
Zhong
Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, School of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, PR China.
Zhi-lei
ZL
Tan
D016667:Bacterial Capsules / Q000378:metabolism / Q000528:radiation effects*; D045604:Extracellular Fluid; D008027:Light; D046937:Nostoc; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis / Q000302:isolation & purification* / Q000528:radiation effects*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D014961:X-Ray Diffraction
D011135:Polysaccharides, Bacterial
Cyanobacteria; Light wavelength; Nostoc flagelliforme; Polysaccharide