DVD technology-based molecular diagnosis platform: quantitative pregnancy test on a disc.
A diagnosis platform based entirely on DVD technology was developed for on-site quantitation of molecular analytes of interest, e.g., human chorionic gonadotropin (hCG) in urine samples ("quantitative pregnancy test on a disc"). An hCG-specific monoclonal antibody-binding assay prepared on a regular DVD-R was labeled with nanogold-streptavidin conjugates for signal enhancement with a customized silver-staining protocol. An unmodified, conventional computer optical drive was used for assay reading, and free disc-quality analysis software for data processing. The performance (sensitivity and selectivity) of this DVD assay is comparable to that of well-established colorimetric methods (determination of optical darkness ratios) and standard enzyme-linked immunosorbent assays (ELISA). As validated by examining its linear correlation with the ELISA results on the same set of samples, the DVD assay promises to be a low-cost, multiplex, point-of-care (POC) diagnostic tool for physicians and even for individuals at home, producing prompt results.
Laboratory of Advanced Transducers and Intelligent Control Systems (Ministry of Education), College of Physics and Optoelectronics, Taiyuan University of Technology, Shanxi 030024, China. hogan_yu@sfu.ca lixiaochun@tyut.edu.cn.
Xiaochun
X
Li
Samuel
S
Weng
Bixia
B
Ge
Zhihui
Z
Yao
Hua-Zhong
HZ
Yu
D000911:Antibodies, Monoclonal / Q000276:immunology; D006063:Chorionic Gonadotropin / Q000276:immunology / Q000652:urine*; D003201:Computers; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation / Q000379:methods*; D005260:Female; D006046:Gold / Q000737:chemistry; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry / Q000378:metabolism; D007834:Lasers; D053768:Metal Nanoparticles / Q000737:chemistry; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation; D010084:Oxidation-Reduction; D019095:Point-of-Care Systems; D011247:Pregnancy; D011258:Pregnancy Tests; D012984:Software; D019809:Streptavidin / Q000737:chemistry / Q000378:metabolism
D000911:Antibodies, Monoclonal; D006063:Chorionic Gonadotropin; D055767:Immobilized Proteins; D006046:Gold; D019809:Streptavidin