Aceriphyllum rossii extract and its active compounds, quercetin and kaempferol inhibit IgE-mediated mast cell activation and passive cutaneous anaphylaxis.
Aceriphyllum rossii contains an abundant source of natural flavonoids with potential antioxidant, anticancer and anti-inflammatory properties. However, the effect of A. rossii extract (ARE) on immunoglobulin E(IgE)-mediated allergic responses remains unknown. In the present study, the effects of ARE and its active compounds, quercetin and kaempferol, on IgE-mediated rat basophilic leukemia mast cell activation and passive cutaneous anaphylaxis (PCA) were investigated. ARE, quercetin, and kaempferol inhibited secretion of β-hexosaminidase and histamine, and reduced the production and mRNA expression of interleukin-4 and tumor necrosis factor-α. ARE also decreased the production of prostaglandin E2 and leukotriene B4 and expression of cyclooxygenase 2 and 5-lipoxygenase. Furthermore, ARE, quercetin, and kaempferol inhibited IgE-mediated phosphorylation of Syk, phospholipase Cγ, protein kinase C (PKC)μ, and the mitogen-activated protein kinases, extracellular signal-regulated kinase, p38, and c-Jun N-terminal kinase. ARE, quercetin, and kaempferol markedly suppressed mast cell-dependent PCA in IgE-sensitized mice. These results indicate that ARE and its active constituents, quercetin and kaempferol, may be a useful therapy for immediate-type hypersensitivity.
Functional Food Center, Korea Institute of Science and Technology (KIST), Gangneung Institute , Gangwon Korea.
Myungsuk
M
Kim
Sue Ji
SJ
Lim
Suk Woo
SW
Kang
Byung-Hun
BH
Um
Chu Won
CW
Nho
D000707:Anaphylaxis / Q000188:drug therapy* / Q000276:immunology; D000818:Animals; D018926:Anti-Allergic Agents / Q000008:administration & dosage*; D006632:Histamine / Q000276:immunology; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology*; D015847:Interleukin-4 / Q000276:immunology; D044949:Kaempferols / Q000008:administration & dosage*; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010936:Plant Extracts / Q000008:administration & dosage*; D011794:Quercetin / Q000008:administration & dosage*; D051381:Rats; D031995:Saxifragaceae; D012867:Skin / Q000187:drug effects / Q000276:immunology*
D018926:Anti-Allergic Agents; D044949:Kaempferols; D010936:Plant Extracts; D015847:Interleukin-4; D007073:Immunoglobulin E; C006552:kaempferol; D006632:Histamine; D011794:Quercetin