Genome engineering with TALENs and ZFNs: repair pathways and donor design.
Genome engineering with targetable nucleases depends on cellular pathways of DNA repair after target cleavage. Knowledge of how those pathways work, their requirements and their active factors, can guide experimental design and improve outcomes. While many aspects of both homologous recombination (HR) and nonhomologous end joining (NHEJ) are shared by a broad range of cells and organisms, some features are specific to individual situations. This article reviews the influence of repair mechanisms on the results of gene targeting experiments, with an emphasis on lessons learned from experiments with Drosophila.
Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT 84112-5650, USA. Electronic address: dana@biochem.utah.edu.
Dana
D
Carroll
Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT 84112-5650, USA.
Kelly J
KJ
Beumer
D000818:Animals; D059766:DNA End-Joining Repair / Q000235:genetics*; D004330:Drosophila; D029721:Drosophila Proteins / Q000235:genetics*; D005818:Genetic Engineering / Q000379:methods*; D016678:Genome; D016335:Zinc Fingers / Q000235:genetics*
D029721:Drosophila Proteins
DNA repair; Gene targeting; Homologous recombination; NHEJ; TALENs; ZFNs