Optimization of polysaccharides from Zagros oak leaf using RSM: antioxidant and antimicrobial activities.
Ultrasonic assisted-extraction technique was applied to extract the polysaccharide from Zagros oak (Quercus brantii Lindl). The effects of four independent factors (ultrasonic power (X1: 150-300 W), extraction temperature (X2: 50-90°C), extraction time (X3: 30-90 min), and the ratio of water to raw material (X4: 15-45)) on the extraction yield of polysaccharide from the leaves of Q. brantii Lindl (QBLP) were optimized using response surface methodology. The experimental data obtained were fitted to a second-order polynomial equation. The optimal extraction conditions for QBLP were determined as follows: X1: 205.8 W, X2: 81.9°C, X3: 55.6 min and X4: 23.4. Under these optimal conditions, the experimental yield was 19.42 ± 0.53%, which was well matched with the value predicted by the model 19.61%. The results indicated that polysaccharide has strong scavenging activities in vitro on DPPH and hydroxyl radicals. In addition, the QBLP showed good antimicrobial activity at 1.5-2.5mg/mL.
Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Ramin Agriculture and Natural Resources University, Mollasani, Ahvaz, Iran. Electronic address: saeed_tahmouzi@yahoo.com.
Saeed
S
Tahmouzi
D000890:Anti-Infective Agents / Q000302:isolation & purification* / Q000494:pharmacology; D000975:Antioxidants / Q000302:isolation & purification* / Q000494:pharmacology; D002176:Candida albicans / Q000187:drug effects; D002458:Cell Fractionation / Q000379:methods; D052978:Disk Diffusion Antimicrobial Tests; D016166:Free Radical Scavengers / Q000302:isolation & purification / Q000494:pharmacology; D017665:Hydroxyl Radical / Q000032:analysis; D010407:Penicillium / Q000187:drug effects; D018515:Plant Leaves / Q000737:chemistry; D011134:Polysaccharides / Q000302:isolation & purification* / Q000494:pharmacology; D029963:Quercus / Q000737:chemistry*; D012485:Salmonella typhi / Q000187:drug effects; D012995:Solubility; D013010:Sonication / Q000379:methods*; D013211:Staphylococcus aureus / Q000187:drug effects; D013696:Temperature; D014867:Water
D000890:Anti-Infective Agents; D000975:Antioxidants; D016166:Free Radical Scavengers; D011134:Polysaccharides; D014867:Water; D017665:Hydroxyl Radical
Antimicrobial; Antioxidant; Optimization; Polysaccharide; Quercus brantii