Effects of sodium pyruvate on ameliorating metabolic acidosis.
OBJECTIVE
To examine the effects of sodium pyruvate (SP) on metabolic acidosis.


METHODS
For the in vivo experiments, we evaluated effects of SP on an ammonium chloride (NH4Cl)-induced hyperchloremic acidosis rat model. SP was infused at overall doses of 2, 4, and 6 mmol·kg(- 1) for the SP1, SP2, and SP3 groups, respectively. Treatment with sodium bicarbonate (SB) was used as a positive control (2 mmol·kg(- 1)), and treatment with normal saline (NS) was used as a volume control (2 mL·kg(- 1)). Blood was sampled from the ophthalmic venous plexus for pH, blood gases, electrolytes, glucose, creatinine (Cr), and urea analysis after injection. For the in vitro experiment, propionate was applied to induce intracellular acidosis in human endothelial cells. Intracellular pH (pHi) was fluorimetrically measured after the addition of SP.


RESULTS
In the in vivo study, the pH of SP1 group showed no significant difference compared with that of the NS group. The SP2 and SP3 groups had a higher pH than the NS group (P < 0.01). The SP3 group had a higher pH than the SB group (P < 0.05) and SP1 group (P < 0.05). Moreover, SP treatment ameliorated the abnormality of calcium and decreased the blood potassium levels. The SP3 group had higher glucose levels than SP1 group (P < 0.05). No significant differences were observed between all the groups in the plasma Cr and urea levels. In the in vitro study, the pHi increased immediately after the addition of SP.


CONCLUSIONS
The data suggest that intravascular treatment with SP represents a novel therapeutic strategy to ameliorate metabolic acidosis.
a Institute of Transfusion Medicine, Academy of Military Medical Sciences , Beijing , P.R. China.
Jing
J
Yang
a Institute of Transfusion Medicine, Academy of Military Medical Sciences , Beijing , P.R. China.
Jing-Xiang
JX
Zhao
a Institute of Transfusion Medicine, Academy of Military Medical Sciences , Beijing , P.R. China.
Ying
Y
Wang
a Institute of Transfusion Medicine, Academy of Military Medical Sciences , Beijing , P.R. China.
Gan
G
Chen
b Department of Biological Engineering , College of Environment and Chemical Engineering, Yanshan University , Qinhuangdao , P. R. China.
Wei-Na
WN
Cheng
a Institute of Transfusion Medicine, Academy of Military Medical Sciences , Beijing , P.R. China.
Xin
X
Luo
a Institute of Transfusion Medicine, Academy of Military Medical Sciences , Beijing , P.R. China.
Xue-Tao
XT
Pei
a Institute of Transfusion Medicine, Academy of Military Medical Sciences , Beijing , P.R. China.
Lian
L
Zhao
c The First Affiliated Hospital of General Hospital of PLA , Beijing , P. R. China.
Qin
Q
Su
a Institute of Transfusion Medicine, Academy of Military Medical Sciences , Beijing , P.R. China.
Hong
H
Zhou
D000136:Acid-Base Equilibrium / Q000187:drug effects; D000138:Acidosis / Q000097:blood* / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D000643:Ammonium Chloride; D000818:Animals; D001784:Blood Gas Analysis; D001786:Blood Glucose / Q000378:metabolism; D002118:Calcium / Q000097:blood; D002460:Cell Line; D003404:Creatinine / Q000097:blood; D042783:Endothelial Cells / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D011188:Potassium / Q000097:blood; D019289:Pyruvic Acid / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012964:Sodium / Q000097:blood; D017693:Sodium Bicarbonate / Q000494:pharmacology; D014508:Urea / Q000097:blood
D001786:Blood Glucose; D000643:Ammonium Chloride; D019289:Pyruvic Acid; D017693:Sodium Bicarbonate; D014508:Urea; D012964:Sodium; D003404:Creatinine; D011188:Potassium; D002118:Calcium
blood gases; glycometabolism; metabolic acidosis; pyruvate