Down-regulation of Sox11 is required for efficient osteogenic differentiation of adipose-derived stem cells.
Adipose-derived stem cells represent a type of mesenchymal stem cells with the attendant capacity to self-renew and differentiate into multiple cell lineages. We have performed a microarray-based gene expression profiling of osteogenic differentiation and found that the transcription factor Sox11 is down-regulated during the process. Functional assays demonstrate that down-regulation of Sox11 is required for an efficient differentiation. Furthermore, results from forced expression of constitutively-active and dominant-negative derivatives of Sox11 indicate that Sox11 functions as a transcriptional activator in inhibiting osteogenesis. Sox11 thus represents a novel regulator of osteogenesis whose expression and activity can be potentially manipulated for controlled differentiation.
Department of Life Science, Ewha Womans University, Seoul 120-750, Korea.
Mi Kyung
MK
Choi
Department of Life Science, Ewha Womans University, Seoul 120-750, Korea.
Ikjoo
I
Seong
Ewha Research Center for Systems Biology, Seoul 120-750, Korea.
Seon Ah
SA
Kang
Jaesang
J
Kim
D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D019070:Cell Lineage / Q000235:genetics; D002478:Cells, Cultured; D015536:Down-Regulation; D020869:Gene Expression Profiling; D008297:Male; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D046228:Microarray Analysis; D009154:Mutation / Q000235:genetics; D010012:Osteogenesis / Q000235:genetics; D055751:SOXC Transcription Factors / Q000235:genetics / Q000378:metabolism*; D015533:Transcriptional Activation / Q000235:genetics
D055751:SOXC Transcription Factors