Synthesis and in vitro evaluation of bis-quaternary 2-(hydroxyimino)-N-(pyridin-3-yl)acetamide derivatives as reactivators against sarin and VX inhibited human acetylcholinesterase (hAChE).
A series of bis-quaternary pyridinium derivatives 3a-3i of 2-(hydroxyimino)-N-(pyridin-3-yl)acetamide (2) have been synthesized. The synthesized pyridinium compounds have an amide group in conjugation to the oxime moiety. These compounds were evaluated in vitro for their reactivation efficacy against organophosphorus (OP) nerve agents (NAs) (sarin and VX) inhibited human erythrocyte ghost acetylcholinesterase (hAChE) and compared with the reactivation efficacy of 2-PAM and obidoxime. The pKa values of the synthesized compounds were found closer to the pKa values of 2- and 4-pyridinium oxime reactivators such as 2-PAM and obidoxime. Some of the compounds have shown better reactivation efficacy than 2-PAM, and obidoxime against sarin and VX inhibited AChE.
Process Technology Development Division, Defence Research & Development Establishment, Jhansi Road, Gwalior 474 002, India.
Hitendra N
HN
Karade
Process Technology Development Division, Defence Research & Development Establishment, Jhansi Road, Gwalior 474 002, India.
Aditya Kapil
AK
Valiveti
Process Technology Development Division, Defence Research & Development Establishment, Jhansi Road, Gwalior 474 002, India. Electronic address: jracharya01@gmail.com.
Jyotiranjan
J
Acharya
Process Technology Development Division, Defence Research & Development Establishment, Jhansi Road, Gwalior 474 002, India.
Mahabir Parshad
MP
Kaushik
D000081:Acetamides / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D000110:Acetylcholinesterase / Q000737:chemistry / Q000378:metabolism*; D057075:Enzyme Assays; D006801:Humans; D007700:Kinetics; D009946:Organothiophosphorus Compounds / Q000737:chemistry* / Q000378:metabolism; D010091:Oximes / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D011725:Pyridines / Q000737:chemistry*; D012524:Sarin / Q000737:chemistry* / Q000378:metabolism
D000081:Acetamides; D000110:Acetylcholinesterase; D009946:Organothiophosphorus Compounds; D010091:Oximes; D011725:Pyridines; D012524:Sarin; C009680:VX-agent; C023666:pyridine
2-(Hydroxyimino)-N-(pyridin-3-yl)acetamide; Acetylcholinesterase; Nerve agents; Organophosphorus; Reactivators