Analysis of miRNAs and their targets during adventitious shoot organogenesis of Acacia crassicarpa.
Organogenesis is an important process for plant regeneration by tissue or cell mass differentiation to regenerate a complete plant. MicroRNAs (miRNAs) play an essential role in regulating plant development by mediating target genes at transcriptional and post-transcriptional levels, but the diversity of miRNAs and their potential roles in organogenesis of Acacia crassicarpa have rarely been investigated. In this study, approximately 10 million sequence reads were obtained from a small RNA library, from which 189 conserved miRNAs from 57 miRNA families, and 7 novel miRNAs from 5 families, were identified from A. crassicarpa organogenetic tissues. Target prediction for these miRNAs yielded 237 potentially unique genes, of which 207 received target Gene Ontology annotations. On the basis of a bioinformatic analysis, one novel and 13 conserved miRNAs were selected to investigate their possible roles in A. crassicarpa organogenesis by qRT-PCR. The stage-specific expression patterns of the miRNAs provided information on their possible regulatory functions, including shoot bud formation, modulated function after transfer of the culture to light, and regulatory roles during induction of organogenesis. This study is the first to investigate miRNAs associated with A. crassicarpa organogenesis. The results provide a foundation for further characterization of miRNA expression profiles and roles in the regulation of diverse physiological pathways during adventitious shoot organogenesis of A. crassicarpa.
College of Biological Sciences and Technology, Beijing Forestry University, Beijing, PR China.
Weina
W
Liu
College of Biological Sciences and Technology, Beijing Forestry University, Beijing, PR China.
Wangning
W
Yu
College of Biological Sciences and Technology, Beijing Forestry University, Beijing, PR China.
Lingyu
L
Hou
College of Biological Sciences and Technology, Beijing Forestry University, Beijing, PR China.
Xiaoyu
X
Wang
College of Biological Sciences and Technology, Beijing Forestry University, Beijing, PR China.
Fei
F
Zheng
College of Biological Sciences and Technology, Beijing Forestry University, Beijing, PR China.
Weixuan
W
Wang
College of Biological Sciences and Technology, Beijing Forestry University, Beijing, PR China.
Di
D
Liang
College of Biological Sciences and Technology, Beijing Forestry University, Beijing, PR China.
Hailun
H
Yang
College of Biological Sciences and Technology, Beijing Forestry University, Beijing, PR China.
Yi
Y
Jin
College of Biological Sciences and Technology, Beijing Forestry University, Beijing, PR China.
Xiangming
X
Xie
D000045:Acacia / Q000235:genetics* / Q000254:growth & development*; D001483:Base Sequence; D016000:Cluster Analysis; D017124:Conserved Sequence / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D038081:Organogenesis / Q000235:genetics*; D063245:Plant Development / Q000235:genetics; D018520:Plant Shoots / Q000235:genetics* / Q000254:growth & development; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017423:Sequence Analysis, RNA
D035683:MicroRNAs; D020033:Protein Isoforms; D018749:RNA, Plant