Adsorption behavior of tightly bound extracellular polymeric substances on model organic surfaces under different pH and cations with surface plasmon resonance.
Tightly bound extracellular polymeric substances (TB-EPS) play a substantial role on microbial aggregates, which can promote microbial cells to aggregate and adhere onto the carrier in bioreactor. However, the attachment and adsorption of TB-EPS on different surfaces were awaited to be elucidated. In this study, four self-assembled monolayers (SAMs) carrying methyl (CH3-SAM), amino (NH2-SAM), hydroxyl (OH-SAM), and carboxyl (COOH-SAM) terminal groups were prepared to model different surfaces. TB-EPS adsorption on these surfaces under different pH conditions and additional cations were investigated using surface plasmon resonance. The adsorption of TB-EPS dramatically decreased with the decreasing pH values. CH3-SAM surface achieved the maximum adsorption at the same condition. Na(+) promoted the TB-EPS adsorbed on COOH-SAM surface. The Ca(2+)-mediated complexes were attracted by COOH-SAM and repelled by NH2-SAM, respectively. The adsorptions of TB-EPS on the four SAM surfaces were significantly increased by adding Fe(3+). These results demonstrated that the TB-EPS adsorption on the organic surfaces were dependent on the pH and cation of solution.
Key Laboratory of the Three Gorges Reservoir Region's Eco-Environments of MOE, Chongqing University, Chongqing 400045, China.
Peng
P
Zhang
Key Laboratory of the Three Gorges Reservoir Region's Eco-Environments of MOE, Chongqing University, Chongqing 400045, China; Key Laboratory of Reservoir Aquatic Environment, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, China. Electronic address: ypchen@cigit.ac.cn.
You-Peng
YP
Chen
Key Laboratory of the Three Gorges Reservoir Region's Eco-Environments of MOE, Chongqing University, Chongqing 400045, China; Key Laboratory of Reservoir Aquatic Environment, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, China.
Jin-Song
JS
Guo
Key Laboratory of Reservoir Aquatic Environment, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, China.
Yu
Y
Shen
Key Laboratory of Reservoir Aquatic Environment, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, China.
Ji-Xiang
JX
Yang
Key Laboratory of the Three Gorges Reservoir Region's Eco-Environments of MOE, Chongqing University, Chongqing 400045, China.
Fang
F
Fang
Key Laboratory of the Three Gorges Reservoir Region's Eco-Environments of MOE, Chongqing University, Chongqing 400045, China.
Chun
C
Li
Key Laboratory of the Three Gorges Reservoir Region's Eco-Environments of MOE, Chongqing University, Chongqing 400045, China.
Xu
X
Gao
College of Bioengineering, Chongqing University, Chongqing 400045, China.
Gui-Xue
GX
Wang
D000327:Adsorption; D019149:Bioreactors; D002412:Cations / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D011108:Polymers / Q000737:chemistry*; D020349:Surface Plasmon Resonance; D013499:Surface Properties; D014874:Water Pollutants, Chemical / Q000737:chemistry*
D002412:Cations; D011108:Polymers; D014874:Water Pollutants, Chemical
Adsorption; Extracellular polymeric substances; Self-assembled monolayer; Surface plasmon resonance