The osteoregenerative effects of platelet-derived growth factor BB cotransplanted with mesenchymal stem cells, loaded on freeze-dried mineral bone block: a pilot study in dog mandible.
Due to shortcomings associated with autogenous bone graft, the gold standard of craniofacial grafting, investigators seek alternatives that are accessible, efficient, and affordable. Accordingly, in the present pilot study, bone regeneration was induced using bone marrow-derived mesenchymal stem cells (BMSCs) loaded onto freeze-dried mineral bone block (FDMBB) in the presence or absence of recombinant platelet-derived growth factor-BB (rh PDGF-BB). Eight weeks after the bilateral extraction of premolars of four mongrel dogs, 25 × 10 mm defects were created at both sides of the mandible. The right mandible received autogenous-BMSC loaded on FDMBB (MSC group), whereas the left mandible received cellular blocks impregnated with rhPDGF-BB (MSC + PDGF Group). Animals were euthanized 8 weeks after grafting. Micro-computed tomography (micro-CT) and histomorphometric analysis demonstrated higher levels of bone formation for the test group (10.34% ± 0.20 and 26.63% ± 3.14, respectively) when compared to the control group (8.20% ± 0.20 and 21.38% ± 5.11). The differences were not statistically significant (P > 0.05). According to the performed micro-CT and histomorphometric analysis, adding 0.5 mg rhPDGF-BB (0.3 mg/mL) to the combination of BMSC/FDMBB did not significantly increase bone formation in supracrestal defect in dog mandible.
Department of Oral and Maxillofacial Surgery, Research Institute of Dental Sciences, Dental school, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Arash
A
Khojasteh
Seyedeh Ghazaleh
SG
Dashti
Mohammad Mehdi
MM
Dehghan
Hossein
H
Behnia
Pegah
P
Abbasnia
Golnaz
G
Morad
D043925:Angiogenesis Inducing Agents / Q000494:pharmacology*; D000818:Animals; D064592:Autografts; D000077214:Becaplermin; D001854:Bone Marrow Cells; D001861:Bone Regeneration / Q000187:drug effects*; D004285:Dogs; D005612:Freeze Drying; D008334:Mandible; D008338:Mandibular Injuries / Q000473:pathology / Q000628:therapy*; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells; D010865:Pilot Projects; D020574:Proto-Oncogene Proteins c-sis / Q000494:pharmacology*
D043925:Angiogenesis Inducing Agents; D020574:Proto-Oncogene Proteins c-sis; D000077214:Becaplermin
bone; cell therapy; growth factor; stem cell; tissue engineering