Stachyose: One of the Active Fibroblast-proliferating Components in the Root of Rehmanniae Radix ( dì huáng).
This study aimed to investigate and compare the fibroblast-proliferating activities of different Rehmanniae Radix (RR) samples and its chemical components using human normal fibroblast cells Hs27. Those active components were quantified in differently treated RR samples using UPLC so as to correlate activity with component content. Our results showed that dried RR aqueous extract exhibited the most potent fibroblast-proliferating activity. Stronger effect was observed when ethanol with heating was applied in the extraction process. Stachyose and verbascoside were demonstrated for their first time to exhibit significant stimulatory effects on fibroblast proliferation. However, the proliferating effect of dried RR extract did not correlate with the stachyose content, and verbascoside was not responsible for the fibroblast proliferative effect of RR since it was undetectable in all samples. In conclusion, stachyose only contributed in part to the activity of RR, suggesting that other active components might be present and yet to be found.
Institute of Chinese Medicine; ; State Key Laboratory of Phytochemistry & Plant Resources in West China (CUHK);
Patrick Kwok-Kin
PK
Lai
Institute of Chinese Medicine; ; State Key Laboratory of Phytochemistry & Plant Resources in West China (CUHK);
Ming-Ho
MH
To
Institute of Chinese Medicine; ; State Key Laboratory of Phytochemistry & Plant Resources in West China (CUHK);
Kit-Man
KM
Lau
Institute of Chinese Medicine; ; State Key Laboratory of Phytochemistry & Plant Resources in West China (CUHK);
Cheuk-Lun
CL
Liu
Institute of Chinese Medicine; ; State Key Laboratory of Phytochemistry & Plant Resources in West China (CUHK);
Ling
L
Cheng
Institute of Chinese Medicine; ; State Key Laboratory of Phytochemistry & Plant Resources in West China (CUHK); ; School of Biomedical Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong.
Kwok-Pui
KP
Fung
Institute of Chinese Medicine; ; State Key Laboratory of Phytochemistry & Plant Resources in West China (CUHK);
Ping-Chung
PC
Leung
Institute of Chinese Medicine; ; State Key Laboratory of Phytochemistry & Plant Resources in West China (CUHK);
Clara Bik-San
CB
Lau
Fibroblast proliferation; Rehmannia glutinosa; Rehmanniae Radix; Stachyose; Verbascoside