A paper-based calorimetric microfluidics platform for bio-chemical sensing.
In this report, a paper-based micro-calorimetric biochemical detection method is presented. Calorimetric detection of biochemical reactions is demonstrated as an extension of current colorimetric and electrochemical detection mechanisms of paper-based biochemical analytical systems. Reaction and/or binding temperature of glucose/glucose oxidase, DNA/hydrogen peroxide, and biotin/streptavidin, are measured by the paper-based micro-calorimeter. Commercially available glucose calibration samples of 0.05, 0.15 and 0.3% wt/vol concentration are used for comparing the device performance with a commercially available glucose meter (electrochemical detection). The calorimetric glucose detection demonstrates a measurement error less than 2%. The calorimetric detection results of DNA concentrations from 0.9 to 7.3 mg/mL and temperature changes in biotin and streptavidin reaction are presented to demonstrate the feasibility of integrating the calorimetric detection method with paper based microfluidic devices.
Nanoscale Devices Laboratory, Marquette University, Milwaukee, WI 53233, United States.
Benyamin
B
Davaji
Nanoscale Devices Laboratory, Marquette University, Milwaukee, WI 53233, United States. Electronic address: chunghoon.lee@marquette.edu.
Chung Hoon
CH
Lee
D015374:Biosensing Techniques / Q000295:instrumentation*; D002138:Calibration; D002151:Calorimetry / Q000295:instrumentation*; D003124:Colorimetry; D004247:DNA / Q000032:analysis*; D004867:Equipment Design; D005947:Glucose / Q000032:analysis*; D057230:Limit of Detection; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D010209:Paper
D004247:DNA; D005947:Glucose
Label-free detection; Micro-calorimetry; Paper-based sensor; Thermal bio-sensor