TNF-alpha gene polymorphisms in Iranian Azari Turkish patients with inflammatory bowel diseases.
BACKGROUND
Crohn's disease (CD) and ulcerative colitis (UC) are chronic inflammatory diseases of the bowel (IBD) whose causes are not fully known. Emerging data indicate that alterations in cytokine synthesis may play a role in IBD pathogenesis.


OBJECTIVE
We aimed to determine the association between tumor necrosis factor-alfa (TNFα) promoter polymorphisms (at positions - 308 and - 1031) and susceptibility to IBD among Iranian Azari Turkish patients.


METHODS
One hundred and one patients with IBD and 100 healthy subjects were analyzed.


METHODS
Both polymorphisms in the promoter region of the TNFα gene at positions -1031T/C and -308G/A were detected by polymerase chain reaction-restriction fragment length polymorphism assay. All statistical analyses were calculated with SPSS for Windows 16.0. The Fisher's exact test was used to test for departure from Hardy-Weinberg equilibrium of the genotype frequencies (P > 0.05).


RESULTS
The allele frequency of the TNFα-308G and -1031T were higher in IBD patients but did not reach statistical significance. However, the homozygous TT genotype for the SNP-1031 T > C was significantly higher in UC patients than in healthy controls (P = 0.01) and the heterozygous CT genotype for the SNP -1031 T > C was significantly lower in UC patients than in healthy controls (P = 0.03).


CONCLUSIONS
The TNFα-1031 T allele confers a significant risk for developing UC in Iranian Azeri Turkish patients. Also the frequency of TNFα-1031 C allele was considerably low among patients with UC and it may have protective role among them (OR = 0.43; P = 0.01).
Liver and Gastrointestinal Disease Research Centre of Tabriz University of Medical Sciences, Tabriz, Iran.
Mortaza
M
Bonyadi
Reza
R
Abdolmohammadi
Zohreh
Z
Jahanafrooz
Mohammad-Hosein
MH
Somy
Manoochehr
M
Khoshbaten
D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D003093:Colitis, Ulcerative / Q000235:genetics; D003424:Crohn Disease / Q000235:genetics; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D016036:Seroepidemiologic Studies; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*; D014421:Turkey / Q000208:ethnology; D055815:Young Adult
D014409:Tumor Necrosis Factor-alpha