Detection of follicular transport of lidocaine and metabolism in adipose tissue in pig ear skin by DESI mass spectrometry imaging.
Desorption electrospray ionization (DESI) mass spectrometry imaging is demonstrated as a detection technique for penetration experiments of drugs in skin. Lidocaine ointment was used as the model compound in ex vivo experiments with whole pig ears as the skin model. Follicular transport of lidocaine into the deeper skin layers is demonstrated for the first time. Furthermore, metabolism of lidocaine to 3-OH-lidocaine was observed in subcutaneous tissue as well as in lobules of white adipose tissue surrounding the hair follicles. These results suggest that it is advantageous to use full thickness skin, including subcutaneous tissue, for skin metabolism studies.
Department of Pharmacy, University of Copenhagen, Universitetsparken 2, Copenhagen, 2100, Denmark.
Janina
J
D'Alvise
Rasmus
R
Mortensen
Steen H
SH
Hansen
Christian
C
Janfelt
D000273:Adipose Tissue / Q000187:drug effects*; D000287:Administration, Topical; D000818:Animals; D001692:Biological Transport; D004423:Ear; D004817:Epidermis / Q000378:metabolism; D008012:Lidocaine / Q000032:analysis* / Q000737:chemistry; D012867:Skin / Q000187:drug effects*; D012997:Solvents / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D013552:Swine
D012997:Solvents; D008012:Lidocaine