Identification of key genes in the response to Salmonella enterica Enteritidis, Salmonella enterica Pullorum, and poly(I:C) in chicken spleen and caecum.
Salmonella enterica Enteritidis (S. Enteritidis) and Salmonella enterica Pullorum (S. pullorum) are regarded as a threat to poultry production. This study's aim is to characterize the expression profiles in response to three different challenges and to identify infection-related genes in the chicken spleen and caecum. Groups of the Chinese chicken breed Langshan were challenged with either S. Enteritidis, S. pullorum, or poly(I:C). The concentrations of cytokines and antibodies and the Salmonella colonization level of the caecum and liver were detected in each group at 7 days postinfection. Expression microarray experiments were conducted using mRNA isolated from both spleen and caecum. Crucial differentially expressed genes (DEGs) associated with immunity were identified. Four DEGs were identified in spleen of all three challenge groups (RBM16, FAH, SOX5, and RBM9) and different four genes in caecum (SOUL, FCN2, ANLN, and ACSL1). Expression profiles were clearly different among the three challenged groups. Genes enriched in the spleen of birds infected with S. pullorum were enriched in lymphocyte proliferation related pathways, but the enriched genes in the caecum of the same group were primarily enriched in innate immunity or antibacterial responses. The DEGs that appear across all three challenge groups might represent global response factors for different pathogens.
Animal Genetic Resources Laboratory, College of Animal Science and Technology, Yangzhou University, 88 South of University Avenue, Yangzhou, Jiangsu 225009, China.
Teng
0000-0003-4397-4035
T
Ma
Animal Genetic Resources Laboratory, College of Animal Science and Technology, Yangzhou University, 88 South of University Avenue, Yangzhou, Jiangsu 225009, China.
Guobin
0000-0002-5915-3752
G
Chang
Animal Genetic Resources Laboratory, College of Animal Science and Technology, Yangzhou University, 88 South of University Avenue, Yangzhou, Jiangsu 225009, China.
Rong
0000-0003-2132-4656
R
Chen
Animal Genetic Resources Laboratory, College of Animal Science and Technology, Yangzhou University, 88 South of University Avenue, Yangzhou, Jiangsu 225009, China.
Zhongwei
Z
Sheng
Animal Genetic Resources Laboratory, College of Animal Science and Technology, Yangzhou University, 88 South of University Avenue, Yangzhou, Jiangsu 225009, China.
Aiqin
A
Dai
Animal Genetic Resources Laboratory, College of Animal Science and Technology, Yangzhou University, 88 South of University Avenue, Yangzhou, Jiangsu 225009, China.
Fei
F
Zhai
Animal Genetic Resources Laboratory, College of Animal Science and Technology, Yangzhou University, 88 South of University Avenue, Yangzhou, Jiangsu 225009, China.
Jianchao
J
Li
Animal Genetic Resources Laboratory, College of Animal Science and Technology, Yangzhou University, 88 South of University Avenue, Yangzhou, Jiangsu 225009, China.
Mingxiu
M
Xia
Animal Genetic Resources Laboratory, College of Animal Science and Technology, Yangzhou University, 88 South of University Avenue, Yangzhou, Jiangsu 225009, China.
Dengke
D
Hua
Animal Genetic Resources Laboratory, College of Animal Science and Technology, Yangzhou University, 88 South of University Avenue, Yangzhou, Jiangsu 225009, China.
Lu
L
Xu
Animal Genetic Resources Laboratory, College of Animal Science and Technology, Yangzhou University, 88 South of University Avenue, Yangzhou, Jiangsu 225009, China.
Hongzhi
H
Wang
Animal Genetic Resources Laboratory, College of Animal Science and Technology, Yangzhou University, 88 South of University Avenue, Yangzhou, Jiangsu 225009, China.
Jing
J
Chen
Animal Genetic Resources Laboratory, College of Animal Science and Technology, Yangzhou University, 88 South of University Avenue, Yangzhou, Jiangsu 225009, China.
Lu
L
Liu
Animal Genetic Resources Laboratory, College of Animal Science and Technology, Yangzhou University, 88 South of University Avenue, Yangzhou, Jiangsu 225009, China.
Guohong
G
Chen
D000818:Animals; D002432:Cecum / Q000276:immunology* / Q000382:microbiology; D002645:Chickens / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D016207:Cytokines / Q000235:genetics / Q000276:immunology; D007113:Immunity, Innate / Q000235:genetics / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology; D011201:Poultry Diseases / Q000235:genetics / Q000276:immunology / Q000382:microbiology; D012333:RNA, Messenger / Q000235:genetics; D012480:Salmonella Infections / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D019779:Salmonella enterica / Q000276:immunology*; D013154:Spleen / Q000276:immunology* / Q000382:microbiology; D059467:Transcriptome / Q000235:genetics* / Q000276:immunology
D016207:Cytokines; D012333:RNA, Messenger