Cell-based flavivirus infection (CFI) assay for the evaluation of dengue antiviral candidates using high-content imaging.
Large-scale screening of antiviral compounds that target dengue virus life cycle requires a robust cell-based assay that is rapid, easy to conduct, and sensitive enough to be able to assess viral infectivity and cell viability so that antiviral efficacy can be measured. In this chapter we describe a method that uses high-content imaging to evaluate the in vitro antiviral efficacy in a modification to the cell-based flavivirus immunodetection (CFI) assay that was described previously in Wang et al. (Antimicrob Agents Chemother 53(5):1823-1831, 2009).
Emerging Infectious Diseases Program, Duke-NUS Graduate Medical School, Singapore, Singapore.
Kah Hin
KH
Tan
Kitti Chan Wing
KC
Ki
Satoru
S
Watanabe
Subhash G
SG
Vasudevan
Manoj
M
Krishnan
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology* / Q000627:therapeutic use; D002460:Cell Line; D002470:Cell Survival; D003715:Dengue / Q000188:drug therapy / Q000821:virology*; D003716:Dengue Virus / Q000187:drug effects*; D057166:High-Throughput Screening Assays / Q000379:methods*; D021621:Imaging, Three-Dimensional; D007211:Indoles / Q000378:metabolism; D013194:Staining and Labeling; D013223:Statistics as Topic
D000998:Antiviral Agents; D007211:Indoles; C007293:DAPI