Microbial profile of root canals of primary teeth with pulp necrosis and periradicular lesion.
OBJECTIVE
The purpose of this study was to assess the microbial content of root canals of human primary teeth with pulp necrosis and periradicular lesion.


METHODS
Microbial samples were collected from 24 canals of children treated at a pediatric dentistry clinic. Microbiological identification was performed using checker-board DNA-DNA hybridization for 40 different bacteria. Data were analyzed per canal based on the mean count and frequency of each bacterial species.


RESULTS
Detectable levels of bacterial species were observed for 35 probes (88%). The most frequent bacteria were Fusobacterium nucleatum sp. nucleatum, Fusobacterium periodonticum, Prevotella melaninogenica, Prevotella nigrescens, and Prevotella intermedia. Facultative species were identified in 20 root canals (83%), anaerobic species were identified in 24 root canals (100%), and aerobic species in 18 root canals (75%). Black-pigmented bacilli were found in 23 samples (96%). The number of different bacterial species detected per canal ranged from five to 33.


CONCLUSIONS
Endodontic infection in primary teeth with pulp necrosis and periradicular lesion is multimicrobial, including aerobic, facultative, and anaerobic micro-organisms.
Private practice, Florianópolis, Santa Catarina, Brazil. ttriches@gmail.com.
Thaisa Cezária
TC
Triches
Department of Periodontology, Dental Research Division, Guarulhos University, Guarulhos, São Paulo, Brazil.
Luciene Cristina
LC
de Figueiredo
Graduate Program in Dentistry, Department of Periodontology, Dental Research Division, Guarulhos University, Guarulhos, São Paulo, Brazil.
Magda
M
Feres
Department of Public Health, at the School of Dentistry, Federal University of Santa Catarina, Florianópolis, Santa Catarina, Brazil.
Sérgio Fernando Torres
SF
de Freitas
Department of Periodontology, at the School of Dentistry, Federal University of Santa Catarina, Florianópolis, Santa Catarina, Brazil.
Gláucia Santos
GS
Zimmermann
Department of Morphological Sciences, at the School of Dentistry, Federal University of Santa Catarina, Florianópolis, Santa Catarina, Brazil.
Mabel Mariela Rodríguez
MM
Cordeiro
D001419:Bacteria / Q000302:isolation & purification*; D002648:Child; D015342:DNA Probes; D003786:Dental Pulp Cavity / Q000382:microbiology*; D003790:Dental Pulp Necrosis / Q000382:microbiology* / Q000601:surgery; D005260:Female; D006801:Humans; D008297:Male; D010485:Periapical Periodontitis / Q000382:microbiology*; D011670:Pulpectomy; D014094:Tooth, Deciduous
D015342:DNA Probes