Evidence of a conserved intrinsically disordered region in the C-terminus of the stringent response protein Rel from mycobacteria.
The RelA/SpoT enzyme produces (p)ppGpp that helps the bacterium survive during stress. The domains present in it are interspersed with connecting linkers whose functions have been poorly elucidated. We rationally analyzed the sequence and structural property of the regulatory C-terminal region in the Rel family of proteins and report the presence of an intrinsically disordered region between two successive domains in this region that are separated by a defined amino acid sequence length. We show that the length and secondary structure of this linker are conserved in Rel proteins, further signifying its importance in rendering flexibility for domain movement and domain-domain interaction.
Microbiology and Molecular Biology Laboratory, Department of Biological Sciences, Indian Institute of Science Education and Research (IISER), Bhopal 462023, India.
Lakhan
L
Ekal
Microbiology and Molecular Biology Laboratory, Department of Biological Sciences, Indian Institute of Science Education and Research (IISER), Bhopal 462023, India.
Bylapudi
B
Ganesh
Microbiology and Molecular Biology Laboratory, Department of Biological Sciences, Indian Institute of Science Education and Research (IISER), Bhopal 462023, India.
Himanshu
H
Joshi
Biomolecular Modeling and Design Division, Bioinformatics Institute, A(*)STAR, Singapore 138671, Singapore. Electronic address: dilrajl@bii.a-star.edu.sg.
Dilraj
D
Lama
Microbiology and Molecular Biology Laboratory, Department of Biological Sciences, Indian Institute of Science Education and Research (IISER), Bhopal 462023, India. Electronic address: vikas@iiserb.ac.in.
Vikas
V
Jain
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry*; D002942:Circular Dichroism; D017124:Conserved Sequence; D057927:Hydrophobic and Hydrophilic Interactions; D064267:Intrinsically Disordered Proteins; D008025:Ligases / Q000737:chemistry*; D008969:Molecular Sequence Data; D020102:Mycobacterium smegmatis / Q000201:enzymology*; D010802:Phylogeny; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D017386:Sequence Homology, Amino Acid
D001426:Bacterial Proteins; D064267:Intrinsically Disordered Proteins; D008025:Ligases; C028812:guanosine 3',5'-polyphosphate synthetases
Intrinsically disordered region; Mycobacteria; RelA/SpoT; Stringent response; ppGpp