Structural characterisation of algae Costaria costata fucoidan and its effects on CCl₄-induced liver injury.
Fucoidan is a well-known natural product that is commonly found in brown algae and shows a variety of activities, including immunomodulation, antioxidation, and the combat of carcinogens. The fucoidan fractions of Costaria costata, a brown algae introduced from Japan and cultured in northern China, were studied. The fucoidan fractions were extracted, separated, and purified using a combinatorial procedure consisting of enzymolysis, ethanol precipitation, and DEAE and size-exclusion chromatographies. The fundamental characteristics of the four enriched fucoidan fractions (F1-F4), such as their sulphate content and monosaccharide composition, were investigated. FTIR and NMR spectroscopy were employed to further elucidate the structural features of the four fractions. It was found that the F1-F4 fractions all showed oxidative activity against hydroxyl radicals. The bioactive effects of the fucoidan fractions on CCl4-induced liver injury suggest their potential use as ingredients for functional foods or pharmaceuticals.
Key Laboratory of Aquatic Products Processing and Utilisation of Leaning Province, Dalian Ocean University, Dalian 116023, PR China. Electronic address: wqk320@dlou.edu.cn.
Qiukuan
Q
Wang
Key Laboratory of Aquatic Products Processing and Utilisation of Leaning Province, Dalian Ocean University, Dalian 116023, PR China. Electronic address: syf@dlou.edu.cn.
Yuefan
Y
Song
Key Laboratory of Aquatic Products Processing and Utilisation of Leaning Province, Dalian Ocean University, Dalian 116023, PR China. Electronic address: hyh@dlou.edu.cn.
Yunhai
Y
He
Key Laboratory of Aquatic Products Processing and Utilisation of Leaning Province, Dalian Ocean University, Dalian 116023, PR China. Electronic address: rdd@dlou.edu.cn.
Dandan
D
Ren
Australian Maritime College, Launceston, Tasmania 7250, Australia. Electronic address: fkow@utas.edu.au.
Felicia
F
Kow
Key Laboratory of Aquatic Products Processing and Utilisation of Leaning Province, Dalian Ocean University, Dalian 116023, PR China. Electronic address: qzy@dlou.edu.cn.
Zhiyong
Z
Qiao
Key Laboratory of Aquatic Products Processing and Utilisation of Leaning Province, Dalian Ocean University, Dalian 116023, PR China. Electronic address: ls@dlou.edu.cn.
Shu
S
Liu
Marine Bioproducts Engineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 116023, PR China. Electronic address: xjy@dicp.ac.cn.
Xingju
X
Yu
D002251:Carbon Tetrachloride / Q000633:toxicity*; D056486:Chemical and Drug Induced Liver Injury / Q000517:prevention & control*; D019610:Cytoprotection / Q000187:drug effects; D017665:Hydroxyl Radical / Q000737:chemistry; D000459:Phaeophyceae / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*
D011134:Polysaccharides; D017665:Hydroxyl Radical; C007789:fucoidan; D002251:Carbon Tetrachloride
Costaria costata; Fucoidan; Liver injury; Structural characterisation