Regulation of NAMPT in human gingival fibroblasts and biopsies.
Adipokines, such as nicotinamide phosphoribosyltransferase (NAMPT), are molecules, which are produced in adipose tissue. Recent studies suggest that NAMPT might also be produced in the tooth-supporting tissues, that is, periodontium, which also includes the gingiva. The aim of this study was to examine if and under what conditions NAMPT is produced in gingival fibroblasts and biopsies from healthy and inflamed gingiva. Gingival fibroblasts produced constitutively NAMPT, and this synthesis was significantly increased by interleukin-1β and the oral bacteria P. gingivalis and F. nucleatum. Inhibition of the MEK1/2 and NFκB pathways abrogated the stimulatory effects of F. nucleatum on NAMPT. Furthermore, the expression and protein levels of NAMPT were significantly enhanced in gingival biopsies from patients with periodontitis, a chronic inflammatory infectious disease of the periodontium, as compared to gingiva from periodontally healthy individuals. In summary, the present study provides original evidence that gingival fibroblasts produce NAMPT and that this synthesis is increased under inflammatory and infectious conditions. Local synthesis of NAMPT in the inflamed gingiva may contribute to the enhanced gingival and serum levels of NAMPT, as observed in periodontitis patients. Moreover, local production of NAMPT by gingival fibroblasts may represent a possible mechanism whereby periodontitis may impact on systemic diseases.
Clinical Research Unit 208, Center of Dento-Maxillo-Facial Medicine, University of Bonn, Welschnonnenstrasse 17, 53111 Bonn, Germany ; Experimental Dento-Maxillo-Facial Medicine, Center of Dento-Maxillo-Facial Medicine, University of Bonn, 53111 Bonn, Germany ; Department of Oral Surgery, Center of Dento-Maxillo-Facial Medicine, University of Bonn, 53111 Bonn, Germany.
Anna
A
Damanaki
Clinical Research Unit 208, Center of Dento-Maxillo-Facial Medicine, University of Bonn, Welschnonnenstrasse 17, 53111 Bonn, Germany ; Experimental Dento-Maxillo-Facial Medicine, Center of Dento-Maxillo-Facial Medicine, University of Bonn, 53111 Bonn, Germany.
Marjan
M
Nokhbehsaim
Department of Periodontology, Laboratory of Oral Microbiology, University of Bern, 3010 Bern, Switzerland.
Sigrun
0000-0002-4619-2461
S
Eick
Clinical Research Unit 208, Center of Dento-Maxillo-Facial Medicine, University of Bonn, Welschnonnenstrasse 17, 53111 Bonn, Germany ; Department of Orthodontics, Center of Dento-Maxillo-Facial Medicine, University of Bonn, 53111 Bonn, Germany.
Werner
W
Götz
Clinical Research Unit 208, Center of Dento-Maxillo-Facial Medicine, University of Bonn, Welschnonnenstrasse 17, 53111 Bonn, Germany ; Department of Periodontology, Operative and Preventive Dentistry, Center of Dento-Maxillo-Facial Medicine, University of Bonn, 53111 Bonn, Germany.
Jochen
J
Winter
Department of Oral Surgery, Center of Dento-Maxillo-Facial Medicine, University of Bonn, 53111 Bonn, Germany.
Gerhard
G
Wahl
Clinical Research Unit 208, Center of Dento-Maxillo-Facial Medicine, University of Bonn, Welschnonnenstrasse 17, 53111 Bonn, Germany ; Department of Orthodontics, Center of Dento-Maxillo-Facial Medicine, University of Bonn, 53111 Bonn, Germany.
Andreas
A
Jäger
Clinical Research Unit 208, Center of Dento-Maxillo-Facial Medicine, University of Bonn, Welschnonnenstrasse 17, 53111 Bonn, Germany ; Department of Periodontology, Operative and Preventive Dentistry, Center of Dento-Maxillo-Facial Medicine, University of Bonn, 53111 Bonn, Germany.
Søren
S
Jepsen
Clinical Research Unit 208, Center of Dento-Maxillo-Facial Medicine, University of Bonn, Welschnonnenstrasse 17, 53111 Bonn, Germany ; Experimental Dento-Maxillo-Facial Medicine, Center of Dento-Maxillo-Facial Medicine, University of Bonn, 53111 Bonn, Germany.
James
0000-0002-8808-8769
J
Deschner
D052242:Adiponectin / Q000378:metabolism; D000293:Adolescent; D000328:Adult; D001706:Biopsy; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism*; D005260:Female; D005347:Fibroblasts / Q000201:enzymology*; D015971:Gene Expression Regulation, Enzymologic; D005881:Gingiva / Q000166:cytology* / Q000201:enzymology; D006801:Humans; D007249:Inflammation; D020738:Leptin / Q000378:metabolism; D008297:Male; D054409:Nicotinamide Phosphoribosyltransferase / Q000378:metabolism*; D010518:Periodontitis / Q000201:enzymology; D052243:Resistin / Q000378:metabolism; D055815:Young Adult
D052242:Adiponectin; D016207:Cytokines; D020738:Leptin; D052243:Resistin; D054409:Nicotinamide Phosphoribosyltransferase; C085455:nicotinamide phosphoribosyltransferase, human