Fever-range temperature modulates activation and function of human dendritic cells stimulated with the pathogenic mould Aspergillus fumigatus.
In immunocompromised patients, invasive aspergillosis (IA) is the most frequent disease caused by the pathogenic mould Aspergillus fumigatus. Fever is one of the most common yet nonspecific clinical symptoms of IA. To evaluate the role of hyperthermia in the innate immune response to A. fumigatus in vitro, human monocyte-derived dendritic cells (DCs) were stimulated with germ tubes of A. fumigatus or the fungal cell wall component zymosan at 37°C or 40°C, followed by characterization of specific DC functions. While maturation of DCs was enhanced and DC phagocytic capacity was reduced at 40°C, we observed that DC viability and cytokine release were unaffected. Thus, our results suggest that hyperthermia has substantial impacts on DC function in vitro, which might also influence the course and outcome of IA in immunocompromised patients.
Medical Clinic II.
Anna
A
Semmlinger
Mirjam
M
Fliesser
Ana Maria
AM
Waaga-Gasser
Mariola
M
Dragan
C Oliver
CO
Morton
Hermann
H
Einsele
Juergen
J
Loeffler
D001232:Aspergillus fumigatus / Q000276:immunology*; D002454:Cell Differentiation / Q000528:radiation effects; D002470:Cell Survival / Q000528:radiation effects; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000276:immunology* / Q000528:radiation effects*; D006358:Hot Temperature; D006801:Humans; D010587:Phagocytosis / Q000528:radiation effects
D016207:Cytokines
Aspergillus fumigatus; dendritic cells; hyperthermia; invasive aspergillosis