Analysis of five alkaloids using surfactant-coated multi-walled carbon nanotubes as the pseudostationary phase in nonaqueous capillary electrophoresis.
In this paper, surfactant-coated multi-walled carbon nanotubes (SC-MWNTs) have been proposed as a novel pseudostationary phase (PSP) to enhance the separation of isoquinoline alkaloids in nonaqueous capillary electrophoresis (NACE). Several parameters affecting NACE separation were studied including the MWNT concentration, the electrolyte concentration, pH* and the separation voltage. In comparison to conventional NACE, the addition of an MWNT dispersion using surfactant solutions in the electrolyte produced an important enhancement in the resolution due to the π-π interactions between the analytes and the surface of the carbon nanotubes. Using SC-MWNTs (6μgmL(-1)) as a PSP in the background electrolyte (BGE) (i.e., 20mM sodium acetate in methanol-acetonitrile (80:20, v/v)) provided the complete separation of five alkaloids. Finally, the developed method has been successfully applied to the detection and quantification of the tested compounds of Rhizoma Coptidis.
Area Major Laboratory of Protection and Utilization of Traditional Chinese Medicine, Beijing Normal University, Haidian District, Beijing 100088, PR China; State Key Laboratory Breeding Base of Dao-di Herbs, China Academy of Chinese Medical Sciences, Beijing 100700, PR China.
Jingyi
J
Hou
Area Major Laboratory of Protection and Utilization of Traditional Chinese Medicine, Beijing Normal University, Haidian District, Beijing 100088, PR China; State Key Laboratory Breeding Base of Dao-di Herbs, China Academy of Chinese Medical Sciences, Beijing 100700, PR China.
Geng
G
Li
Area Major Laboratory of Protection and Utilization of Traditional Chinese Medicine, Beijing Normal University, Haidian District, Beijing 100088, PR China; State Key Laboratory Breeding Base of Dao-di Herbs, China Academy of Chinese Medical Sciences, Beijing 100700, PR China; School of Chemistry and Pharmaceutical Engineering, Qilu University of Technology, Jinan 250353, PR China.
Yingqin
Y
Wei
Area Major Laboratory of Protection and Utilization of Traditional Chinese Medicine, Beijing Normal University, Haidian District, Beijing 100088, PR China; State Key Laboratory Breeding Base of Dao-di Herbs, China Academy of Chinese Medical Sciences, Beijing 100700, PR China.
Heng
H
Lu
Area Major Laboratory of Protection and Utilization of Traditional Chinese Medicine, Beijing Normal University, Haidian District, Beijing 100088, PR China; State Key Laboratory Breeding Base of Dao-di Herbs, China Academy of Chinese Medical Sciences, Beijing 100700, PR China.
Chao
C
Jiang
Area Major Laboratory of Protection and Utilization of Traditional Chinese Medicine, Beijing Normal University, Haidian District, Beijing 100088, PR China; State Key Laboratory Breeding Base of Dao-di Herbs, China Academy of Chinese Medical Sciences, Beijing 100700, PR China.
Xiaoteng
X
Zhou
Area Major Laboratory of Protection and Utilization of Traditional Chinese Medicine, Beijing Normal University, Haidian District, Beijing 100088, PR China; State Key Laboratory Breeding Base of Dao-di Herbs, China Academy of Chinese Medical Sciences, Beijing 100700, PR China. Electronic address: mfy@bnu.edu.cn.
Fanyun
F
Meng
College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 310036, PR China. Electronic address: caojun91@163.com.
Jun
J
Cao
Area Major Laboratory of Protection and Utilization of Traditional Chinese Medicine, Beijing Normal University, Haidian District, Beijing 100088, PR China; State Key Laboratory Breeding Base of Dao-di Herbs, China Academy of Chinese Medical Sciences, Beijing 100700, PR China. Electronic address: liujx_23@163.com.
Jinxin
J
Liu
D000470:Alkaloids / Q000032:analysis*; D029064:Araceae / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000032:analysis; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008855:Microscopy, Electron, Scanning; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D013501:Surface-Active Agents / Q000737:chemistry*
D000470:Alkaloids; D004365:Drugs, Chinese Herbal; D037742:Nanotubes, Carbon; D013501:Surface-Active Agents
Multi-walled carbon nanotubes; Nonaqueous capillary electrophoresis; Rhizoma Coptidis; Surfactant; Surfactant-coated multi-walled carbon nanotubes