Prooxidant effects of verbascoside, a bioactive compound from olive oil mill wastewater, on in vitro developmental potential of ovine prepubertal oocytes and bioenergetic/oxidative stress parameters of fresh and vitrified oocytes.
Verbascoside (VB) is a bioactive polyphenol from olive oil mill wastewater with known antioxidant activity. Oxidative stress is an emerging problem in assisted reproductive technology (ART). Juvenile ART is a promising topic because, in farm animals, it reduces the generation gap and, in human reproductive medicine, it helps to overcome premature ovarian failure. The aim of this study was to test the effects of VB on the developmental competence of ovine prepubertal oocytes and the bioenergetic/oxidative stress status of fresh and vitrified oocytes. In fresh oocytes, VB exerted prooxidant short-term effects, that is, catalase activity increase and uncoupled increases of mitochondria and reactive oxygen species (ROS) fluorescence signals, and long-term effects, that is, reduced blastocyst formation rate. In vitrified oocytes, VB increased ROS levels. Prooxidant VB effects in ovine prepubertal oocytes could be related to higher VB accumulation, which was found as almost one thousand times higher than that reported in other cell systems in previous studies. Also, long exposure times of oocytes to VB, throughout the duration of in vitro maturation culture, may have contributed to significant increase of oocyte oxidation. Further studies are needed to identify lower concentrations and/or shorter exposure times to figure out VB antioxidant effects in juvenile ARTs.
Section of Veterinary Clinics and Animal Production, Department of Emergency and Organ Transplantation (DETO), University of Bari "Aldo Moro", Strada Provincial Casamassima Km 3, Valenzano, 70010 Bari, Italy.
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Dell'Aquila
Obstetric and Gynecological Section, Department of Veterinary Medicine, Via Vienna 2, 07100 Sassari, Italy.
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Bogliolo
Section of Veterinary Clinics and Animal Production, Department of Emergency and Organ Transplantation (DETO), University of Bari "Aldo Moro", Strada Provincial Casamassima Km 3, Valenzano, 70010 Bari, Italy.
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Russo
Section of Veterinary Clinics and Animal Production, Department of Emergency and Organ Transplantation (DETO), University of Bari "Aldo Moro", Strada Provincial Casamassima Km 3, Valenzano, 70010 Bari, Italy.
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Martino
Section of Veterinary Clinics and Animal Production, Department of Emergency and Organ Transplantation (DETO), University of Bari "Aldo Moro", Strada Provincial Casamassima Km 3, Valenzano, 70010 Bari, Italy.
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Filioli Uranio
Obstetric and Gynecological Section, Department of Veterinary Medicine, Via Vienna 2, 07100 Sassari, Italy.
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Ariu
Department of Basic Medical Sciences, Neurosciences and Sense Organs, University of Bari "Aldo Moro", Piazza Giulio Cesare, 70124 Bari, Italy.
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Amati
Department of Basic Medical Sciences, Neurosciences and Sense Organs, University of Bari "Aldo Moro", Piazza Giulio Cesare, 70124 Bari, Italy ; Institute of Translational Pharmacology (IFT), National Research Council (CNR), Via Fosso del Cavaliere No. 100, 00133 Rome, Italy.
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Sardanelli
Institute of Sciences of Food Production (ISPA), National Research Council (CNR), Via G. Amendola 122/O, 70126 Bari, Italy.
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Linsalata
Department of Food Science, 745 Agriculture Mall Drive, West Lafayette, IN 47907, USA.
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Ferruzzi
Institute of Sciences of Food Production (ISPA), National Research Council (CNR), Via G. Amendola 122/O, 70126 Bari, Italy.
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Cardinali
Institute of Sciences of Food Production (ISPA), National Research Council (CNR), Via G. Amendola 122/O, 70126 Bari, Italy.
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Minervini
D000818:Animals; D000890:Anti-Infective Agents / Q000009:adverse effects / Q000494:pharmacology; D005260:Female; D005960:Glucosides / Q000009:adverse effects* / Q000494:pharmacology; D006801:Humans; D008928:Mitochondria / Q000378:metabolism / Q000473:pathology; D000069463:Olive Oil; D009865:Oocytes / Q000378:metabolism* / Q000473:pathology; D016877:Oxidants / Q000009:adverse effects* / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects*; D010636:Phenols / Q000009:adverse effects* / Q000494:pharmacology; D010938:Plant Oils; D017382:Reactive Oxygen Species / Q000378:metabolism; D012756:Sheep; D062065:Wastewater; D059808:Polyphenols
D000890:Anti-Infective Agents; D005960:Glucosides; D000069463:Olive Oil; D016877:Oxidants; D010636:Phenols; D010938:Plant Oils; D017382:Reactive Oxygen Species; D062065:Wastewater; C058956:acteoside; D059808:Polyphenols