Competing endogenous RNA and interactome bioinformatic analyses on human telomerase.
We present a classic interactome bioinformatic analysis and a study on competing endogenous (ce) RNAs for hTERT. The hTERT gene codes for the catalytic subunit and limiting component of the human telomerase complex. Human telomerase reverse transcriptase (hTERT) is essential for the integrity of telomeres. Telomere dysfunctions have been widely reported to be involved in aging, cancer, and cellular senescence. The hTERT gene network has been analyzed using the BioGRID interaction database (http://thebiogrid.org/) and related analysis tools such as Osprey (http://biodata.mshri.on.ca/osprey/servlet/Index) and GeneMANIA (http://genemania.org/). The network of interaction of hTERT transcripts has been further analyzed following the competing endogenous (ce) RNA hypotheses (messenger [m] RNAs cross-talk via micro [mi] RNAs) using the miRWalk database and tools (www.ma.uni-heidelberg.de/apps/zmf/mirwalk/). These analyses suggest a role for Akt, nuclear factor-κB (NF-κB), heat shock protein 90 (HSP90), p70/p80 autoantigen, 14-3-3 proteins, and dynein in telomere functions. Roles for histone acetylation/deacetylation and proteoglycan metabolism are also proposed.
1 Dipartimento Biomedico di Medicina Interna e Specialistica (Biomedical Department of Internal and Specialized Medicine), University of Palermo , Palermo, Italy .
Walter
W
Arancio
Giuseppe
G
Pizzolanti
Swonild Ilenia
SI
Genovese
Concetta
C
Baiamonte
Carla
C
Giordano
D019295:Computational Biology / Q000379:methods*; D005786:Gene Expression Regulation; D006801:Humans; D011485:Protein Binding; D012313:RNA / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D019098:Telomerase / Q000235:genetics / Q000378:metabolism*
D012333:RNA, Messenger; D012313:RNA; C509186:TERT protein, human; D019098:Telomerase