Protective effect of Disporum sessile D.Don extract against UVB-induced photoaging via suppressing MMP-1 expression and collagen degradation in human skin cells.
In the present study, we report that Disporum sessile D.Don herbal extract (DDE) possesses anti-skin photoaging effect through inhibition of MMP-1 mRNA and protein expression levels and increase collagen production in UVB-irradiated human dermal fibroblast cells (NHDF). To delineate the molecular mechanism by which DDE inhibited MMP-1 expression, immortal human keratinocytes cells (HaCaT) have been used. We have found that DDE inhibited UVB-induced MMP-1 mRNA and protein expression levels in HaCaT cells through inhibition of UVB-induced activation of NF-κB in HaCaT cells. Inhibitors of NF-κB (Bay11-7082), and mitogen-activated protein kinases such as extracellular regulated kinase (PD98059), c-Jun N-terminal kinase (SP600125), and p38 (SB203580) suppressed expression of MMP-1, and phosphorylation of these signaling molecules were attenuated by DDE. DDE also inhibited phosphorylation of IKKα and IκBα, and reduced nuclear translocation of NF-κB. Our results also demonstrated that DDE inhibited NF-κB driven expression of luciferase reporter gene and the DNA binding of NF-κB to its cognate binding site in UV-irradiated cells. Therefore, these results strongly suggest that DDE can be utilized as a potential agent for prevention and treatment of skin photoaging.
College of Life Sciences and Graduate School of Biotechnology Kyung Hee University, Yongin 4460-701, Republic of Korea.
Mohamed Antar Aziz
MA
Mohamed
College of Life Sciences and Graduate School of Biotechnology Kyung Hee University, Yongin 4460-701, Republic of Korea.
Mira
M
Jung
College of Life Sciences and Graduate School of Biotechnology Kyung Hee University, Yongin 4460-701, Republic of Korea.
Sang Min
SM
Lee
College of Life Sciences and Graduate School of Biotechnology Kyung Hee University, Yongin 4460-701, Republic of Korea. Electronic address: thlee@khu.ac.kr.
Tae Hoon
TH
Lee
College of Life Sciences and Graduate School of Biotechnology Kyung Hee University, Yongin 4460-701, Republic of Korea. Electronic address: jkim@khu.ac.kr.
Jiyoung
J
Kim
D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects; D015870:Gene Expression / Q000187:drug effects* / Q000528:radiation effects; D006801:Humans; D051550:I-kappa B Kinase / Q000378:metabolism; D020672:I-kappa B Proteins / Q000378:metabolism; D019668:Liliaceae / Q000737:chemistry* / Q000378:metabolism; D020781:Matrix Metalloproteinase 1 / Q000235:genetics / Q000378:metabolism*; D020929:Mitogen-Activated Protein Kinase Kinases / Q000378:metabolism; D000072000:NF-KappaB Inhibitor alpha; D016328:NF-kappa B / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects / Q000528:radiation effects; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects / Q000528:radiation effects; D014466:Ultraviolet Rays
D020672:I-kappa B Proteins; D016328:NF-kappa B; C000606204:NFKBIA protein, human; D010936:Plant Extracts; D012333:RNA, Messenger; D000072000:NF-KappaB Inhibitor alpha; D003094:Collagen; D051550:I-kappa B Kinase; D020929:Mitogen-Activated Protein Kinase Kinases; D020781:Matrix Metalloproteinase 1
Collagen; Disporum sessile D.Don extract; MMP-1; NF-κB; UVB-irradiation