[Application of multiplex PCR for the screening of genotyping system for the rare blood groups Fy(a-), s-,k-,Di(b-) and Js(b-)].
OBJECTIVE
To screen rare blood groups Fy(a-), s-, k-, Di(b-) and Js(b-) in an ethnic Zhuang population.


METHODS
Sequence-specific primers were designed based on single nucleotide polymorphism (SNP) sites of blood group antigens Fy(b) and s. A specific multiplex PCR system I was established. Multiplex PCR system II was applied to detect alleles antigens Di(b), k, Js(b)1910 and Js(b) 2019 at the same time. The two systems was were used to screen for rare blood group antigens in 4490 randomly selected healthy donors of Guangxi Zhuang ethnic origin.


RESULTS
We successfully made the multiplex PCR system I. We detected the rare blood group antigens using the two PCR system. There are five Fy(a-), three s(-), two Di(b-) in 4490 Guangxi zhuang random samples. The multiplex PCR system I has achieved good accuracy and stability. With multiplex PCR systems I and II, 4490 samples were screened. Five Fy(a-), three s(-) and two Di(b-) samples were discovered.


CONCLUSIONS
Multiplex PCR is an effective methods, which can be used for high throughput screening of rare blood groups. The rare blood types of Guangxi Zhuang ethnic origin obtained through the screening can provide valuable information for compatible blood transfusion. Through screening we obtained precious rare blood type materials which can be used to improve the capability of compatible infusion and reduce the transfusion reactions.
Blood Transfusion of Guangxi Zhuang Autonomous Region People's Hospital, Guangxi 530021, P. R. China. gxjw2005@163.com; zhuziyan@sbc.org.cn.
Wei
W
Jiao
Li
L
Xie
Hailan
H
Li
Jiao
J
Lan
Zhuning
Z
Mo
Ziji
Z
Yang
Fei
F
Liu
Ruiping
R
Xiao
Yunlei
Y
He
Luyi
L
Ye
Ziyan
Z
Zhu
D001789:Blood Group Antigens / Q000235:genetics*; D004375:Duffy Blood-Group System / Q000235:genetics*; D005838:Genotype; D006801:Humans; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D011956:Receptors, Cell Surface / Q000235:genetics*
C473745:ACKR1 protein, human; D001789:Blood Group Antigens; D004375:Duffy Blood-Group System; D011956:Receptors, Cell Surface