Surface modification of a polyhedral oligomeric silsesquioxane poly(carbonate-urea) urethane (POSS-PCU) nanocomposite polymer as a stent coating for enhanced capture of endothelial progenitor cells.
An unmet need exists for the development of next-generation multifunctional nanocomposite materials for biomedical applications, particularly in the field of cardiovascular regenerative biology. Herein, we describe the preparation and characterization of a novel polyhedral oligomeric silsesquioxane poly(carbonate-urea) urethane (POSS-PCU) nanocomposite polymer with covalently attached anti-CD34 antibodies to enhance capture of circulating endothelial progenitor cells (EPC). This material may be used as a new coating for bare metal stents used after balloon angioplasty to improve re-endothelialization. Biophysical characterization techniques were used to assess POSS-PCU and its subsequent functionalization with anti-CD34 antibodies. Results indicated successful covalent attachment of anti-CD34 antibodies on the surface of POSS-PCU leading to an increased propensity for EPC capture, whilst maintaining in vitro biocompatibility and hemocompatibility. POSS-PCU has already been used in 3 first-in-man studies, as a bypass graft, lacrimal duct and a bioartificial trachea. We therefore postulate that its superior biocompatibility and unique biophysical properties would render it an ideal candidate for coating medical devices, with stents as a prime example. Taken together, anti-CD34 functionalized POSS-PCU could form the basis of a nano-inspired polymer platform for the next generation stent coatings.
Harvard-MIT Division of Health Sciences & Technology, Cambridge, MA, USA, aaron.tan@ucl.ac.uk.
Aaron
A
Tan
Yasmin
Y
Farhatnia
Debbie
D
Goh
Natasha
N
G
Achala
A
de Mel
Jing
J
Lim
Swee-Hin
SH
Teoh
Andrey V
AV
Malkovskiy
Reema
R
Chawla
Jayakumar
J
Rajadas
Brian G
BG
Cousins
Michael R
MR
Hamblin
Mohammad S
MS
Alavijeh
Alexander M
AM
Seifalian
D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D066026:Endothelial Progenitor Cells / Q000502:physiology*; D006801:Humans; D053761:Nanocomposites / Q000737:chemistry*; D017646:Organosilicon Compounds / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry* / Q000494:pharmacology; D011140:Polyurethanes / Q000737:chemistry*
D017646:Organosilicon Compounds; D011108:Polymers; D011140:Polyurethanes; C110029:poly(carbonate urea) urethane; C521417:polyhedraloligosilsesquioxane