Characterization of developmental- and stress-mediated expression of cinnamoyl-CoA reductase in kenaf (Hibiscus cannabinus L.).
Cinnamoyl-CoA reductase (CCR) is an important enzyme for lignin biosynthesis as it catalyzes the first specific committed step in monolignol biosynthesis. We have cloned a full length coding sequence of CCR from kenaf (Hibiscus cannabinus L.), which contains a 1,020-bp open reading frame (ORF), encoding 339 amino acids of 37.37 kDa, with an isoelectric point (pI) of 6.27 (JX524276, HcCCR2). BLAST result found that it has high homology with other plant CCR orthologs. Multiple alignment with other plant CCR sequences showed that it contains two highly conserved motifs: NAD(P) binding domain (VTGAGGFIASWMVKLLLEKGY) at N-terminal and probable catalytic domain (NWYCYGK). According to phylogenetic analysis, it was closely related to CCR sequences of Gossypium hirsutum (ACQ59094) and Populus trichocarpa (CAC07424). HcCCR2 showed ubiquitous expression in various kenaf tissues and the highest expression was detected in mature flower. HcCCR2 was expressed differentially in response to various stresses, and the highest expression was observed by drought and NaCl treatments.
School of Biotechnology, Yeungnam University, Gyeongsan 712-749, Republic of Korea.
Ritesh
0000-0002-2528-7277
R
Ghosh
School of Biotechnology, Yeungnam University, Gyeongsan 712-749, Republic of Korea.
Bosung
0000-0003-0577-063X
B
Choi
Department of Biosystems Machinery Engineering, Chungnam National University, Daejeon 305-764, Republic of Korea.
Byoung-Kwan
0000-0002-8397-9853
BK
Cho
Department of Applied Biology, Chungnam National University, Daejeon 305-764, Republic of Korea.
Hyoun-Sub
HS
Lim
Department of Crop Science, Chungnam National University, Daejeon 305-754, Republic of Korea.
Sang-Un
SU
Park
Department of Bioenergy Science & Technology, Chonnam National University, Gwangju 500-757, Republic of Korea.
Hyeun-Jong
HJ
Bae
US Department of Agriculture, Agricultural Research Service, Invasive Insects Biocontrol & Behavior Laboratory, 10300 Baltimore Avenue, Beltsville, MD 20705, USA.
Savithiry
S
Natarajan
School of Biotechnology, Yeungnam University, Gyeongsan 712-749, Republic of Korea.
Hanhong
0000-0002-0428-5918
H
Bae
D000445:Aldehyde Oxidoreductases / Q000737:chemistry* / Q000145:classification / Q000378:metabolism*; D000595:Amino Acid Sequence; D018506:Gene Expression Regulation, Plant; D003368:Gossypium / Q000201:enzymology; D031584:Hibiscus / Q000201:enzymology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D032107:Populus / Q000201:enzymology; D017386:Sequence Homology, Amino Acid
D000445:Aldehyde Oxidoreductases; C019904:cinnamoyl CoA reductase