A signal-on fluorescent assay for DNA methyltransferase activity using a methylation-resistant endonuclease.
A simple, rapid, and signal-on fluorescent assay was developed for activity analysis of DNA methyltransferase and for screening of its inhibitors based on a methylation-resistant endonuclease and SYBR Green I.
BioNano Heath Guard Research Center, Korea Research Institute of Bioscience and Biotechnology, P.O Box 125 Gwahak-ro, Yuseong-gu, Daejeon, 305-806, South Korea. chungbh@kribb.re.kr.
Quang Huy
QH
Quach
Bong Hyun
BH
Chung
D019175:DNA Methylation; D015254:DNA Modification Methylases / Q000378:metabolism*; D004720:Endonucleases / Q000378:metabolism*
D015254:DNA Modification Methylases; D004720:Endonucleases