Direct conversion of inulin into cell lipid by an inulinase-producing yeast Rhodosporidium toruloides 2F5.
In this study, an inulinase-producing yeast strain 2F5 of Rhodosporidium toruloides was obtained. It was found that the yeast strain 2F5 could produce higher amount of oil from inulin and larger lipid bodies in its cells than any other yeast strains tested in this study. Under the optimal conditions, 62.14% (w/w) of lipid based on cell dry weight and 15.82g/l of the dry cell mass were produced from 6.0% (w/v) inulin at flask level, leaving 0.92% (w/v) of total sugar in the fermented medium. During 2-l fermentation, 70.36% (w/w) of lipid based on cell dry weight and 15.64g/l of the dry cell mass were produced from 6.0% (w/v) inulin. Over 99.09% of the fatty acids from the yeast strain 2F5 grown on inulin was C16:0, C18:0, C18:1 and C18:2, especially C18:1 (52.2%). The biodiesel prepared using the lipids produced by the yeast strain 2F5 could be burnt well.
Unesco Chinese Center of Marine Biotechnology, Ocean University of China, Yushan Road, No. 5, Qingdao 266003, China.
Zhi-Peng
ZP
Wang
Unesco Chinese Center of Marine Biotechnology, Ocean University of China, Yushan Road, No. 5, Qingdao 266003, China.
Wen-Juan
WJ
Fu
Unesco Chinese Center of Marine Biotechnology, Ocean University of China, Yushan Road, No. 5, Qingdao 266003, China.
Hong-Mei
HM
Xu
Unesco Chinese Center of Marine Biotechnology, Ocean University of China, Yushan Road, No. 5, Qingdao 266003, China. Electronic address: zhenming@sdu.edu.cn.
Zhen-Ming
ZM
Chi
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D005285:Fermentation; D006026:Glycoside Hydrolases / Q000235:genetics / Q000378:metabolism*; D007444:Inulin / Q000378:metabolism*; D050356:Lipid Metabolism; D008969:Molecular Sequence Data; D009821:Oils / Q000378:metabolism*; D015003:Yeasts / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*
D009821:Oils; D007444:Inulin; D006026:Glycoside Hydrolases; C023086:inulinase
Biodiesel; Fermentation; Inulin; Rhodosporidium toruloides; Single cell oil