Evaluation of methods of detecting cell reactive oxygen species production for drug screening and cell cycle studies.
Intracellular reactive oxygen species (ROS) production is essential to normal cell function. However, excessive ROS production causes oxidative damage and cell death. Many pharmacological compounds exert their effects on cell cycle progression by changing intracellular redox state and in many cases cause oxidative damage leading to drug cytotoxicity. Appropriate measurement of intracellular ROS levels during cell cycle progression is therefore crucial in understanding redox-regulation of cell function and drug toxicity and for the development of new drugs. However, due to the extremely short half-life of ROS, measuring the changes in intracellular ROS levels during a particular phase of cell cycle for drug intervention can be challenging. In this article, we have provided updated information on the rationale, the applications, the advantages and limitations of common methods for screening drug effects on intracellular ROS production linked to cell cycle study. Our aim is to facilitate biomedical scientists and researchers in the pharmaceutical industry in choosing or developing specific experimental regimens to suit their research needs.
John Radcliffe Hospital, University of Oxford, Oxford OX3 9DU, UK.
Lampson M
LM
Fan
Faculty of Health and Medical Sciences, University of Surrey, Guildford, UK. Electronic address: j.li@surrey.ac.uk.
Jian-Mei
JM
Li
D002453:Cell Cycle / Q000187:drug effects*; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D006207:Half-Life; D006801:Humans; D010084:Oxidation-Reduction; D004364:Pharmaceutical Preparations / Q000008:administration & dosage; D017382:Reactive Oxygen Species / Q000378:metabolism*
D004364:Pharmaceutical Preparations; D017382:Reactive Oxygen Species
Cell cycle; Cytotoxicity; Drug screen; Methods; Reactive oxygen species