Transplantation with cultured stem cells derived from the human amniotic membrane for corneal alkali burns: an experimental study.
Amniotic membranes (AM) have been used in a wide range of clinical applications. We successfully extracted mesenchymal stem cells (MSCs) from human AM, but little is known about the use and efficacy of human amniotic membrane-derived mesenchymal stem cells (hAM-dMSCs) for the treatment of alkali burns. We utilized hAM-dMSCs transplantation, AM grafting, and their combined use in the treatment of alkali burns. An experimental model in rabbits was devised to analyze the use of these techniques with immunocytochemistry and ELISA. The survival and migration of hAM-dMSCs labeled by SPION in the host were assessed with Prussian blue staining. Compared with the control group, the treated groups demonstrated faster reconstruction of the corneal epithelium, and lower levels of corneal opacification and neovascularization within corneal alkali burns. Furthermore, dark blue-stained particles were detected in the limbus corneae at day 28. These results demonstrated the ability of hAM-dMSCs to enhance epithelial healing and reduce corneal opacification and neovascularization in corneal alkali wounds.
Department of Ophthalmology, Affiliated First Hospital of Zhengzhou University, Zhengzhou 450052, China; phone:+86 13233958993; e mail: lylyw78@163.com.
Wei
W
Zeng
Yanwei
Y
Li
Guangwei
G
Zeng
Bo
B
Yang
Yu
Y
Zhu
D000468:Alkalies; D000650:Amnion / Q000166:cytology*; D000818:Animals; D002056:Burns / Q000473:pathology / Q000628:therapy*; D002465:Cell Movement; D049109:Cell Proliferation; D002478:Cells, Cultured; D016510:Corneal Neovascularization / Q000473:pathology / Q000628:therapy; D003318:Corneal Opacity; D003911:Dextrans / Q000378:metabolism; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D019573:Epithelium, Corneal / Q000473:pathology*; D005260:Female; D006801:Humans; D007249:Inflammation / Q000473:pathology; D058185:Magnetite Nanoparticles; D008297:Male; D011817:Rabbits; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014945:Wound Healing
D000468:Alkalies; D003911:Dextrans; D058185:Magnetite Nanoparticles; D014409:Tumor Necrosis Factor-alpha; C065507:ferumoxides
Amniotic membrane; alkali burns; mesenchymal stem cells