Systematically organized nanopillar arrays reveal differences in adhesion and alignment properties of BMSC and Saos-2 cells.
Polymeric test surfaces of P(L-D,L)LA and of a P(L-D,L)LA:PLGA blend decorated with 25 nanopillar covered fields, were used to investigate differences in growth of bone marrow stem cells (BMSC) and osteosarcoma cells (Saos-2). The fields were populated with pillars (ca. 900 nm tall, 200 nm×200 nm area) separated systematically from each other with 1-10 μm gaps. Saos-2 cells populated fields decorated with pillars 1 μm apart but they avoided pillar-free surfaces. In contrast, BMSCs avoided fields with interpillar distances <2 μm. Both BMSCs and Saos-2 cells aligned in the direction of the shorter distance when at least one of the interpillar distances was greater than 1.5 μm. Coating the P(L-D,L)LA surfaces with cell adhesive protein fibronectin enabled the BMSC to populate fields with high pillar density which they had avoided when uncoated. Decreasing the stiffness of the film surface by using a blend of (P(L-D,L)LA and PLGA) made them more acceptable for attachment by the BMSC cells.
METU, BIOMAT, Department of Biological Sciences, Biotechnology Research Unit, Ankara 06800, Turkey; BIOMATEN, METU Center of Excellence in Biomaterials and Tissue Engineering, Ankara 06800, Turkey.
Hayriye
H
Özçelik
Lab for Micro- and Nanotechnology, Paul Scherrer Institute, 5232 Villigen PSI, Switzerland.
Celestino
C
Padeste
METU, BIOMAT, Department of Biological Sciences, Biotechnology Research Unit, Ankara 06800, Turkey; BIOMATEN, METU Center of Excellence in Biomaterials and Tissue Engineering, Ankara 06800, Turkey. Electronic address: vhasirci@metu.edu.tr.
Vasif
V
Hasirci
D000818:Animals; D001672:Biocompatible Materials; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry / Q000494:pharmacology*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D009928:Organ Specificity; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D011091:Polyesters; D011100:Polyglycolic Acid / Q000737:chemistry / Q000494:pharmacology*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology*; D061251:Primary Cell Culture; D051381:Rats; D017207:Rats, Sprague-Dawley; D013499:Surface Properties; D054457:Tissue Scaffolds
D001672:Biocompatible Materials; D011091:Polyesters; D011108:Polymers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; C033616:poly(lactide)
Anisotropy; Cell alignment; Nanopillar array; Selective cell adhesion; Stiffness