PET Demonstrates Functional Recovery after Treatment by Danhong Injection in a Rat Model of Cerebral Ischemic-Reperfusion Injury.
This study aimed to investigate neuroprotection of Danhong injection (DHI) in a rat model of cerebral ischemia using (18)F-fluorodeoxyglucose positron emission tomography ((18)F-FDG-PET). Method. Rats were divided into 5 groups: sham group, ischemia-reperfusion untreated (IRU) group, DHI-1 group (DHI 1 mL/kg/d), DHI-2 group (DHI 2 mL/kg/d), and DHI-4 group (DHI 4 mL/kg/d). AII the treated groups were intraperitoneally injected with DHI daily for 14 days. The therapeutic effects in terms of cerebral infarct volume, neurological function, and cerebral glucose metabolism were evaluated. Expression of TNF-α and IL-1β was detected with enzyme-linked immunosorbent assay (ELISA). Levels of mature neuronal marker (NeuN), glial marker (GFAP), vascular density factor (vWF), and glucose transporter 1 (GLUT1) were assessed by immunohistochemistry. Results. Compared with the IRU group, rats treated with DHI showed dose dependent reductions in cerebral infarct volume and levels of proinflammatory cytokines, improvement of neurological function, and recovery of cerebral glucose metabolism. Meanwhile, the significantly increased numbers of neurons, gliocytes, and vessels and the recovery of glucose utilization were found in the peri-infarct region after DHI treatment using immunohistochemical analysis. Conclusion. This study demonstrated the metabolic recovery after DHI treatment by micro-PET imaging with (18)F-FDG and the neuroprotective effects of DHI in a rat model of cerebral ischemic-reperfusion injury.
Institute of Cardio-Cerebrovascular Diseases, Zhejiang Chinese Medical University, 548 Binwen Road, Hangzhou, Zhejiang 310053, China ; Department of Nuclear Medicine, The Second Affiliated Hospital of Zhejiang University School of Medicine, 88 Jiefang Road, Hangzhou, Zhejiang 310009, China ; Zhejiang University Medical PET Center, Zhejiang University, Hangzhou 310009, China ; Key Laboratory of Medical Molecular Imaging of Zhejiang Province, Hangzhou 310009, China.
Zefeng
0000-0002-3089-6886
Z
Wang
Department of Nuclear Medicine, The Second Affiliated Hospital of Zhejiang University School of Medicine, 88 Jiefang Road, Hangzhou, Zhejiang 310009, China ; Zhejiang University Medical PET Center, Zhejiang University, Hangzhou 310009, China ; Key Laboratory of Medical Molecular Imaging of Zhejiang Province, Hangzhou 310009, China.
Fahuan
0000-0002-4110-5912
F
Song
Department of Nuclear Medicine, The Second Affiliated Hospital of Zhejiang University School of Medicine, 88 Jiefang Road, Hangzhou, Zhejiang 310009, China ; Zhejiang University Medical PET Center, Zhejiang University, Hangzhou 310009, China ; Key Laboratory of Medical Molecular Imaging of Zhejiang Province, Hangzhou 310009, China.
Jinhui
J
Li
Institute of Cardio-Cerebrovascular Diseases, Zhejiang Chinese Medical University, 548 Binwen Road, Hangzhou, Zhejiang 310053, China.
Yuyan
Y
Zhang
Institute of Cardio-Cerebrovascular Diseases, Zhejiang Chinese Medical University, 548 Binwen Road, Hangzhou, Zhejiang 310053, China.
Yu
Y
He
Institute of Cardio-Cerebrovascular Diseases, Zhejiang Chinese Medical University, 548 Binwen Road, Hangzhou, Zhejiang 310053, China.
Jiehong
J
Yang
Institute of Cardio-Cerebrovascular Diseases, Zhejiang Chinese Medical University, 548 Binwen Road, Hangzhou, Zhejiang 310053, China.
Huifen
0000-0001-8762-8519
H
Zhou
Buchang Pharmaceutical Co., Ltd., Xi'an 712000, China.
Tao
T
Zhao
Buchang Pharmaceutical Co., Ltd., Xi'an 712000, China.
Wei
W
Fu
Buchang Pharmaceutical Co., Ltd., Xi'an 712000, China.
Panke
P
Xing
Institute of Cardio-Cerebrovascular Diseases, Zhejiang Chinese Medical University, 548 Binwen Road, Hangzhou, Zhejiang 310053, China.
Haitong
H
Wan
Department of Nuclear Medicine, The Second Affiliated Hospital of Zhejiang University School of Medicine, 88 Jiefang Road, Hangzhou, Zhejiang 310009, China ; Zhejiang University Medical PET Center, Zhejiang University, Hangzhou 310009, China ; Key Laboratory of Medical Molecular Imaging of Zhejiang Province, Hangzhou 310009, China.
Mei
M
Tian
Department of Nuclear Medicine, The Second Affiliated Hospital of Zhejiang University School of Medicine, 88 Jiefang Road, Hangzhou, Zhejiang 310009, China ; Zhejiang University Medical PET Center, Zhejiang University, Hangzhou 310009, China ; Key Laboratory of Medical Molecular Imaging of Zhejiang Province, Hangzhou 310009, China.
Hong
H
Zhang