Structural identification and sulfated modification of an antiglycation Dendrobium huoshanense polysaccharide.
Dendrobium huoshanense, an important food material, has been used to make teas and soups in the folk of China for centuries. In the present study, an antiglycation polysaccharide DHPD2 with molecular weight of 8.09 × 10(6)Da was extracted from the protocorm-like bodies of D. huoshanense. The backbone of DHPD2 contained (1→5)-linked α-l-Araf, (1→6)-linked α-d-Glcp, (1→6)-linked β-d-Glcp, (1→4)-linked β-d-Glcp, (1→3,6)-linked β-d-Galp and (1→6)-linked β-d-Galp, with the branches of terminal α-d-Xlyp and β-d-Manp. DHPD2 was further modified using chlorosulfonic acid-pyridine method, giving two sulfated derivatives with the substitution degree of 0.475 and 0.940. The appearance of two new characteristic absorption bands at near 1250 and 822cm(-1) in FT-IR spectra revealed the success of sulfation occurred to DHPD2. Moreover, the sulfated derivatives exhibited stronger inhibitory abilities on protein glycation than those of DHPD2. NMR analysis disclosed that the sulfation on C2 and C6 of sugar residues was beneficial to enhance this activity.
School of Biotechnology and Food Engineering, Hefei University of Technology, No. 193, Tunxi Road, Hefei 230009, People's Republic of China.
Xiao-Long
XL
Li
School of Biotechnology and Food Engineering, Hefei University of Technology, No. 193, Tunxi Road, Hefei 230009, People's Republic of China.
Jing-Jing
JJ
Xiao
School of Biotechnology and Food Engineering, Hefei University of Technology, No. 193, Tunxi Road, Hefei 230009, People's Republic of China. Electronic address: xueqiang.zha@upr.edu.
Xue-Qiang
XQ
Zha
School of Biotechnology and Food Engineering, Hefei University of Technology, No. 193, Tunxi Road, Hefei 230009, People's Republic of China.
Li-Hua
LH
Pan
School of Biotechnology and Food Engineering, Hefei University of Technology, No. 193, Tunxi Road, Hefei 230009, People's Republic of China.
Muhammad-Naeem
MN
Asghar
School of Biotechnology and Food Engineering, Hefei University of Technology, No. 193, Tunxi Road, Hefei 230009, People's Republic of China. Electronic address: jianpingluo@hfut.edu.cn.
Jian-Ping
JP
Luo
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002848:Chromatography, DEAE-Cellulose; D031667:Dendrobium / Q000737:chemistry*; D004040:Dietary Carbohydrates / Q000378:metabolism; D017127:Glycation End Products, Advanced / Q000037:antagonists & inhibitors*; D006031:Glycosylation; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010945:Plants, Edible / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification*; D011725:Pyridines / Q000494:pharmacology; D012710:Serum Albumin, Bovine / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared; D013329:Structure-Activity Relationship; D013431:Sulfates / Q000378:metabolism; D013451:Sulfonic Acids / Q000494:pharmacology
D004040:Dietary Carbohydrates; D017127:Glycation End Products, Advanced; D011134:Polysaccharides; D011725:Pyridines; D013431:Sulfates; D013451:Sulfonic Acids; D012710:Serum Albumin, Bovine; C013880:chlorosulfonic acid; C023666:pyridine
Antiglycation; Dendrobium huoshanense; Polysaccharide; Sulfation