Functional annotation of putative hypothetical proteins from Candida dubliniensis.
An extensive analysis of C. dubliniensis proteomics data showed that ~22% protein are conserved hypothetical proteins (HPs) whose function is still not determined precisely. Analysis of gene sequence of HPs provides a platform to establish sequence-function relationships to a more profound understanding of the molecular machinery of organisms at systems level. Here we have combined the latest versions of bioinformatics tools including, protein family, motifs, intrinsic features from the amino acid sequence, sequence-function relationship, pathway analysis, etc. to assign a precise function to HPs for which no any experimental information is available. Our results show that 27 HPs have well defined functions and we categorized them as enzyme, nucleic acid binding, transport protein, etc. Five HPs showed adhesin character that is likely to be essential for the survival of yeast and pathogenesis. We also addressed issues related to the sub-cellular localization and signal peptide identification which provides an idea about its colocalization and function. The outcome of the present study may facilitate better understanding of mechanism of virulence, drug resistance, pathogenesis, adaptability to host, tolerance for host immune response, and drug discovery for treatment of C. dubliniensis infections.
Center for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, Jamia Nagar, New Delhi 110025, India.
Kundan
K
Kumar
Center for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, Jamia Nagar, New Delhi 110025, India.
Amresh
A
Prakash
Center for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, Jamia Nagar, New Delhi 110025, India.
Munazzah
M
Tasleem
Center for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, Jamia Nagar, New Delhi 110025, India.
Asimul
A
Islam
Center for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, Jamia Nagar, New Delhi 110025, India.
Faizan
F
Ahmad
Center for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, Jamia Nagar, New Delhi 110025, India. Electronic address: mihassan@jmi.ac.in.
Md Imtaiyaz
MI
Hassan
D002175:Candida / Q000235:genetics*; D002352:Carrier Proteins / Q000235:genetics; D015815:Cell Adhesion Molecules / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics*; D006867:Hydrolases / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics; D058977:Molecular Sequence Annotation; D010088:Oxidoreductases / Q000235:genetics; D054464:Peroxiredoxins / Q000235:genetics; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics; D010770:Phosphotransferases / Q000235:genetics; D017422:Sequence Analysis, DNA; D014166:Transferases / Q000235:genetics
D002352:Carrier Proteins; D015815:Cell Adhesion Molecules; D005656:Fungal Proteins; D008565:Membrane Proteins; D010088:Oxidoreductases; D054464:Peroxiredoxins; D014166:Transferases; D010770:Phosphotransferases; D006867:Hydrolases; D010744:Phosphoric Monoester Hydrolases
Candida dubliniensis; Functional annotation; Functional genomics; Hypothetical protein; Sequence analysis