Efficient synthesis of protein mimics by sequential native chemical ligation.
Synthetic mimics of protein surfaces have the potential to become inhibitors of protein-protein interactions or even synthetic vaccines. However, the synthesis of these complicated molecular constructs is still difficult. Here we describe an efficient and versatile synthesis of protein mimics containing up to three different cyclic peptides. Using a sequential native chemical ligation strategy, peptide loops containing a thioester handle were introduced onto a triazacyclophane scaffold bearing orthogonal protected cysteine residues.
Medicinal Chemistry & Chemical Biology, Utrecht Institute for Pharmaceutical Sciences, Department of Pharmaceutical Sciences, Faculty of Sciences, Utrecht University , P.O. Box 80082, 3508 TB, Utrecht, The Netherlands.
Helmus
H
van de Langemheen
H Linda C
HL
Quarles van Ufford
John A W
JA
Kruijtzer
Rob M J
RM
Liskamp
D001372:Aza Compounds / Q000737:chemistry*; D003545:Cysteine / Q000737:chemistry; D006574:Heterocyclic Compounds, 2-Ring / Q000737:chemistry*; D015394:Molecular Structure; D010456:Peptides, Cyclic / Q000138:chemical synthesis* / Q000737:chemistry
D001372:Aza Compounds; D006574:Heterocyclic Compounds, 2-Ring; D010456:Peptides, Cyclic; C539946:triazacyclophane; D003545:Cysteine