Comparing microspheres with different internal phase of polyelectrolyte as local drug delivery system for bone tuberculosis therapy.
We use hydrophobic poly(lactic-co-glycolic) acid (PLGA) to encapsulate hydrophilic ofloxacin to form drug loading microspheres. Hyaluronic acid (HA) and polylysine (Pls) were used as internal phase additives to see their influences on the drug loading and releasing. Double emulsion (water-in-oil-in-water) solvent extraction/evaporation method was used for the purpose. Particle size analysis display that the polyelectrolytes have low impact on the microsphere average size and distribution. Scanning electron microscope (SEM) pictures show the wrinkled surface resulted by the internal microcavity of the microspheres. Microspheres with HA inside have higher drug loading amounts than microspheres with Pls inside. The loading drug amounts of the microspheres increase with the HA amounts inside, while decreasing with the Pls amounts inside. All the polyelectrolytes adding groups have burst release observed in experiments. The microspheres with Pls internal phase have faster release rate than the HA groups. Among the same polyelectrolyte internal phase groups, the release rate increases with the amounts increasing when Pls is inside, while it decreases with the amounts increasing when HA is inside.
School of Materials Science and Engineering, South China University of Technology, Guangzhou 510640, China ; National Engineering Center for Tissue Restoration and Reconstruction, Guangzhou 510006, China.
Gang
0000-0002-4730-4971
G
Wu
School of Materials Science and Engineering, South China University of Technology, Guangzhou 510640, China ; National Engineering Center for Tissue Restoration and Reconstruction, Guangzhou 510006, China.
Long
L
Chen
Department of Materials Science and Engineering, Jinan University, Guangzhou 510632, China.
Hong
H
Li
School of Materials Science and Engineering, South China University of Technology, Guangzhou 510640, China ; National Engineering Center for Tissue Restoration and Reconstruction, Guangzhou 510006, China.
Chun-Ling
CL
Deng
School of Materials Science and Engineering, South China University of Technology, Guangzhou 510640, China ; National Engineering Center for Tissue Restoration and Reconstruction, Guangzhou 510006, China.
Xiao-Feng
XF
Chen
D016503:Drug Delivery Systems / Q000379:methods; D004655:Emulsions / Q000737:chemistry / Q000494:pharmacology; D006820:Hyaluronic Acid / Q000737:chemistry; D019344:Lactic Acid / Q000737:chemistry; D008863:Microspheres; D015242:Ofloxacin / Q000737:chemistry / Q000494:pharmacology; D009821:Oils / Q000737:chemistry; D010316:Particle Size; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011107:Polylysine / Q000737:chemistry; D011108:Polymers / Q000737:chemistry* / Q000494:pharmacology*; D012997:Solvents / Q000737:chemistry; D014394:Tuberculosis, Osteoarticular / Q000188:drug therapy*; D014867:Water / Q000737:chemistry
D004655:Emulsions; D009821:Oils; D011108:Polymers; D012997:Solvents; D014867:Water; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011107:Polylysine; D011100:Polyglycolic Acid; D019344:Lactic Acid; D006820:Hyaluronic Acid; D015242:Ofloxacin