Rapid separation of mononuclear hodgkin from multinuclear reed-sternberg cells.
We describe a method to isolate small mononucleated Hodgkin (H) cells from multinucleated Reed Sternberg (RS) cells of Hodgkin lymphoma using the ScreenCell filter device. This filtration-based approach lends itself to future clinical applications in that it enables the separation of H and RS cells from lymph node biopsies, bone marrow aspirates, pleural effusions, and blood, including the isolation of monoclonal Hodgkin precursor cells from the blood.
Manitoba Institute of Cell Biology, Cancer Care Manitoba, The Genomic Centre for Cancer Research and Diagnosis, The University of Manitoba, Winnipeg, Manitoba, Canada Department of Laboratory Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
Narisorn
N
Kongruttanachok
Hôpital Robert Debré and Pierre, Marie Curie University, Paris, France.
Yvon E
YE
Cayre
Division of Haematology/Oncology, CHUS University Hospital, Sherbrooke, Quebec, Canada.
Hans
H
Knecht
Manitoba Institute of Cell Biology, Cancer Care Manitoba, The Genomic Centre for Cancer Research and Diagnosis, The University of Manitoba, Winnipeg, Manitoba, Canada.
Sabine
S
Mai
D045744:Cell Line, Tumor; D002469:Cell Separation / Q000379:methods; D006689:Hodgkin Disease / Q000473:pathology*; D006801:Humans; D016539:Reed-Sternberg Cells / Q000473:pathology*; D021701:Sentinel Lymph Node Biopsy
Hodgkin; Reed Sternberg; cell separation; filtration device