Development of a routine analysis of 4-mercapto-4-methylpentan-2-one in wine by stable isotope dilution assay and mass tandem spectrometry.
The 4-mercapto-4-methylpentan-2-one (4MMP) is a key aroma compound in wines, especially in Sauvignon Blanc ones. Its accurate quantification is quite difficult due to its traces levels and its reactivity in wine conferred by the thiol function. In this paper, we proposed a new method for its quantification in wine without any sample preparation, based on automated derivatization procedure by methoximation and SIDA-SPME-GC-MS/MS analysis. The derivatization procedure was adapted from a previously published method in order to decrease the amount of reagents and the volume of wine (only 3mL are required). The use of SPME and the detection conditions have also been optimized to reach the best sensitivity as possible. The method was then validated according to the International Organization of Vine and Wine recommendations and exhibited excellent performances. Indeed, this method allowed us to quantify the 4MMP in wine at traces levels (LOD=0.19 ng L(-1)) with reproducible results (RSD<15%) and a very good accuracy (recovery=102%).
Nyseos, 2 place Pierre Viala, 34060 Montpellier Cedex 1, France.
Laurent
L
Dagan
Nyseos, 2 place Pierre Viala, 34060 Montpellier Cedex 1, France.
Florence
F
Reillon
Nyseos, 2 place Pierre Viala, 34060 Montpellier Cedex 1, France.
Aurélie
A
Roland
Nyseos, 2 place Pierre Viala, 34060 Montpellier Cedex 1, France. Electronic address: remi@nyseos.fr.
Rémi
R
Schneider
D004867:Equipment Design; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D007201:Indicator Dilution Techniques; D007554:Isotopes / Q000032:analysis; D057230:Limit of Detection; D009812:Odorants / Q000032:analysis*; D010422:Pentanones / Q000032:analysis*; D052616:Solid Phase Extraction / Q000295:instrumentation / Q000379:methods; D013438:Sulfhydryl Compounds / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D027843:Vitis / Q000737:chemistry; D014920:Wine / Q000032:analysis*
C501638:4-mercapto-4-methyl-pentan-2-one; D007554:Isotopes; D010422:Pentanones; D013438:Sulfhydryl Compounds
4-mercapto-4-methylpentan-2-one; Solid phase micro extraction; Stable isotope dilution assay; Validation; Varietal thiol; Wine