Protein-protein interactions leading to recruitment of the host DNA sliding clamp by the hyperthermophilic Sulfolobus islandicus rod-shaped virus 2.
Viruses infecting hyperthermophilic archaea typically do not encode DNA polymerases, raising questions regarding their genome replication. Here, using a yeast two-hybrid approach, we have assessed interactions between proteins of Sulfolobus islandicus rod-shaped virus 2 (SIRV2) and the host-encoded proliferating cell nuclear antigen (PCNA), a key DNA replication protein in archaea. Five SIRV2 proteins were found to interact with PCNA, providing insights into the recruitment of host replisome for viral DNA replication.
New England Biolabs, Ipswich, Massachusetts, USA.
Andrew F
AF
Gardner
Molecular and Cellular Biochemistry Department and Biology Department, Indiana University, Bloomington, Indiana, USA.
Stephen D
SD
Bell
Biomedical Sciences Research Complex, University of St Andrews, Fife, United Kingdom.
Malcolm F
MF
White
Institut Pasteur, Unité Biologie Moléculaire du Gène chez les Extrêmophiles, Département de Microbiologie, Paris, France krupovic@pasteur.fr david.prangishvili@pasteur.fr.
David
D
Prangishvili
Institut Pasteur, Unité Biologie Moléculaire du Gène chez les Extrêmophiles, Département de Microbiologie, Paris, France krupovic@pasteur.fr david.prangishvili@pasteur.fr.
Mart
M
Krupovic
D000595:Amino Acid Sequence; D019843:Archaeal Proteins / Q000235:genetics / Q000378:metabolism*; D019641:DNA, Archaeal / Q000235:genetics / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D023642:Rudiviridae / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016944:Sulfolobus / Q000235:genetics / Q000378:metabolism* / Q000821:virology*; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014779:Virus Replication
D019843:Archaeal Proteins; D019641:DNA, Archaeal; D018809:Proliferating Cell Nuclear Antigen; D014764:Viral Proteins