Polymorphisms of immunity genes and susceptibility to otitis media in children.
BACKGROUND
Acute otitis media (OM) is a common disease which often develops through complex interactions between the host, the pathogen and environmental factors. We studied single nucleotide polymorphisms (SNPs) of genes involved in innate and adaptive immunity, and other host and environmental factors for their role in OM.


METHODS
Using Sequenom Massarray platform, 21 SNPs were studied in 653 children from prospective (n = 202) and retrospective (n = 451) cohorts. Data were analyzed for the relationship between SNPs and upper respiratory infection (URI) frequency, risk of acute OM during URI episodes, and proneness to recurrent OM.


RESULTS
Increased risk for OM proneness was associated with CX3CR1 (Thr280Met) SNP and with a jointly interactive group of IL-10 (-1082) SNP, IL-1β (-511) wild type genotype and white race. Family history of OM proneness independently increased the risk for frequent URIs, OM occurrence during URI, and OM proneness. Additionally, IL-1β (-31) SNP was associated with increased risk for frequent URIs, but IL-10 (-592), IL-1β (-511), IL-5 (-746) and IL-8 (-251) SNPs were associated with decreased risk of URI.


CONCLUSIONS
IL-1β (-31), CX3CR1 (Thr280Met), IL-10 (-1082) and IL-1β (-511) SNPs were associated with increased risk for frequent URIs or OM proneness.
Department of Pediatrics, University of Texas Medical Branch, Galveston, Texas, United States of America.
Johanna
J
Nokso-Koivisto
Department of Pediatrics, University of Texas Medical Branch, Galveston, Texas, United States of America.
Tasnee
T
Chonmaitree
Department of Preventive Medicine and Community Health, University of Texas Medical Branch, Galveston, Texas, United States of America.
Kristofer
K
Jennings
Department of Pediatrics, University of Texas Medical Branch, Galveston, Texas, United States of America.
Reuben
R
Matalon
Kentucky Pediatric Research, Inc., Bardstown, Kentucky, United States of America.
Stan
S
Block
Department of Pediatrics, University of Texas Medical Branch, Galveston, Texas, United States of America.
Janak A
JA
Patel
D056704:Adaptive Immunity / Q000235:genetics*; D000483:Alleles; D000073883:CX3C Chemokine Receptor 1; D002648:Child; D002675:Child, Preschool; D005260:Female; D005500:Follow-Up Studies; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007113:Immunity, Innate / Q000235:genetics*; D007223:Infant; D007378:Interleukins / Q000235:genetics / Q000502:physiology; D008297:Male; D010033:Otitis Media / Q000453:epidemiology / Q000235:genetics* / Q000276:immunology; D020641:Polymorphism, Single Nucleotide; D011446:Prospective Studies; D019707:Receptors, Chemokine / Q000235:genetics / Q000502:physiology; D012141:Respiratory Tract Infections / Q000453:epidemiology / Q000235:genetics / Q000276:immunology; D012189:Retrospective Studies; D012306:Risk; D014777:Virus Diseases / Q000235:genetics / Q000276:immunology
D000073883:CX3C Chemokine Receptor 1; C095235:CX3CR1 protein, human; D007378:Interleukins; D019707:Receptors, Chemokine