Biobleaching of Acacia kraft pulp with extracellular enzymes secreted by Irpex lacteus KB-1.1 and Lentinus tigrinus LP-7 using low-cost media.
The white-rot fungi Irpex lacteus KB-1.1 and Lentinus tigrinus LP-7 have been shown in previous studies to have high biobleaching activity in vivo. The aim of this study was to investigate the activities and stabilities of extracellular enzymes, prepared from I. lacteus and L. tigrinus culture grown in three types of economical media of agricultural and forestry wastes, for biobleaching of Acacia oxygen-delignified kraft pulp using kappa number reduction as an indicator of delignification. After 3 days of incubation, the extracellular enzymes preparations from I. lacteus and L. tigrinus cultures in media of Acacia mangium wood powder supplemented with rice bran and addition 1 % glucose (WRBG), resulted in significant decrease of 4.4 and 6.7 %, respectively. A slightly higher kappa number reduction (7.4 %) was achieved with the combine extracellular enzymes from I. lacteus and L. tigrinus. One of the strategies for reducing the cost of enzyme production for treatment processes in the pulp and paper industry is the utilization of agricultural and forestry waste. Thus, WRBG has potential as a culture medium for producing stable lignolytic enzymes simply and economically.
Laboratory of Plant Nutrition, Graduate School of Agriculture, Hokkaido University, N9W9, Kita-ku, Sapporo, 060-8589, Japan.
Sitompul
S
Afrida
Yutaka
Y
Tamai
Toshihiro
T
Watanabe
Mitsuru
M
Osaki
D000045:Acacia / Q000737:chemistry*; D001673:Biodegradation, Environmental; D003470:Culture Media / Q000737:chemistry* / Q000191:economics; D004795:Enzyme Stability; D005656:Fungal Proteins / Q000096:biosynthesis*; D008031:Lignin / Q000378:metabolism*; D010100:Oxygen; D010209:Paper; D020072:Polyporales / Q000145:classification / Q000201:enzymology*; D014866:Waste Products; D014934:Wood
D003470:Culture Media; D005656:Fungal Proteins; D014866:Waste Products; D008031:Lignin; D010100:Oxygen