Structure and immunobiological activity of a new polysaccharide from Bletilla striata.
A new polysaccharide (designated BSPF2), with a molecular weight of 2.35 × 10(5)Da, was isolated from the tubers of Bletilla striata. It contained mannose, glucose, and galactose in a molar ratio of 9.4:2.6:1.0. The acetyl content of BSPF2 was estimated to be 2.9%, and acetyl groups were located in positions 3 and 6 of mannosyl residues. The structural features were elucidated by a combination of monosaccharide composition analysis, periodate oxidation, partial acid hydrolysis, acetolysis, and methylation analysis. The results indicated that the backbone of BSPF2 consisted of (1→4)-linked mannosyl residues and (1→4)-linked glucosyl residues in a molar ratio of 2:1. About three fifths of glucosyl residues in the backbone were branched at O-6 position, and the terminal sugar residues were composed of mannosyl residues. Immunological assay results demonstrated that BSPF2 significantly induced the spleen cell proliferation in a dose-dependent manner.
College of Food Science and Engineering, Northwest A&F University, YangLing 712100, China. Electronic address: pengqiang@nwsuaf.edu.cn.
Qiang
Q
Peng
College of Resources and Environment, Northwest A&F University, YangLing 712100, China. Electronic address: lileaf@163.com.
Ming
M
Li
College of Food Science and Engineering, Northwest A&F University, YangLing 712100, China. Electronic address: xuefengscholar@126.com.
Feng
F
Xue
College of Resources and Environmental Sciences, Northeast Agricultural University, Harbin 150030, China. Electronic address: huajingliu@neau.edu.cn.
Huajing
H
Liu
D000276:Adjuvants, Immunologic / Q000737:chemistry* / Q000494:pharmacology*; D000818:Animals; D002240:Carbohydrate Sequence; D049109:Cell Proliferation / Q000187:drug effects; D006868:Hydrolysis; D008745:Methylation; D051379:Mice; D008969:Molecular Sequence Data; D029595:Orchidaceae / Q000737:chemistry*; D010084:Oxidation-Reduction; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology*; D013154:Spleen / Q000276:immunology
D000276:Adjuvants, Immunologic; D011134:Polysaccharides
Bletilla striata; Glucomannan; Immunobiological activity; Polysaccharide; Structure