Sequence variation in mitochondrial cox1 and nad1 genes of ascaridoid nematodes in cats and dogs from Iran.
The study was conducted to determine the sequence variation in two mitochondrial genes, namely cytochrome c oxidase 1 (pcox1) and NADH dehydrogenase 1 (pnad1) within and among isolates of Toxocara cati, Toxocara canis and Toxascaris leonina. Genomic DNA was extracted from 32 isolates of T. cati, 9 isolates of T. canis and 19 isolates of T. leonina collected from cats and dogs in different geographical areas of Iran. Mitochondrial genes were amplified by polymerase chain reaction (PCR) and sequenced. Sequence data were aligned using the BioEdit software and compared with published sequences in GenBank. Phylogenetic analysis was performed using Bayesian inference and maximum likelihood methods. Based on pairwise comparison, intra-species genetic diversity within Iranian isolates of T. cati, T. canis and T. leonina amounted to 0-2.3%, 0-1.3% and 0-1.0% for pcox1 and 0-2.0%, 0-1.7% and 0-2.6% for pnad1, respectively. Inter-species sequence variation among the three ascaridoid nematodes was significantly higher, being 9.5-16.6% for pcox1 and 11.9-26.7% for pnad1. Sequence and phylogenetic analysis of the pcox1 and pnad1 genes indicated that there is significant genetic diversity within and among isolates of T. cati, T. canis and T. leonina from different areas of Iran, and these genes can be used for studying genetic variation of ascaridoid nematodes.
Department of Medical Parasitology and Mycology,School of Public Health, Tehran University of Medical Sciences,Tehran,Iran.
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Mikaeili
Department of Medical Parasitology and Mycology,School of Public Health, National Institute of Health Research, Tehran University of Medical Sciences,Tehran,Iran.
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Mirhendi
Department of Medical Parasitology and Mycology,School of Public Health, Tehran University of Medical Sciences,Tehran,Iran.
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Mohebali
Department of Epidemiology and Biostatistics,School of Public Health, Tehran University of Medical Sciences,Tehran,Iran.
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Hosseini
Laboratory Science Research Center, Golestan University of Medical Sciences,Gorgan,Iran.
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Sharbatkhori
Department of Medical Parasitology and Mycology,School of Public Health, Tehran University of Medical Sciences,Tehran,Iran.
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Zarei
Department of Medical Parasitology and Mycology,School of Public Health, Tehran University of Medical Sciences,Tehran,Iran.
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D000818:Animals; D002371:Cat Diseases / Q000453:epidemiology / Q000469:parasitology*; D002415:Cats; D004272:DNA, Mitochondrial / Q000235:genetics; D004283:Dog Diseases / Q000453:epidemiology / Q000469:parasitology*; D004285:Dogs; D003576:Electron Transport Complex IV / Q000235:genetics / Q000378:metabolism*; D007492:Iran / Q000453:epidemiology; D009245:NADH Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D010802:Phylogeny; D017227:Toxascariasis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary; D014114:Toxascaris / Q000235:genetics*; D014119:Toxocara / Q000235:genetics*; D014120:Toxocariasis / Q000453:epidemiology / Q000469:parasitology
D004272:DNA, Mitochondrial; D009245:NADH Dehydrogenase; D003576:Electron Transport Complex IV