Anti-inflammatory effects of Vitis thunbergii var. taiwaniana on knee damage associated with arthritis.
Vitis thunbergii Sieb. et Zucc. var. taiwaniana Lu (VT) is an indigenous plant in Taiwan that is traditionally used for promoting joint health. In this study, we used in vitro primary human chondrocytes (PHCs) and two in vivo animal models to evaluate the anti-inflammatory effects of VT on arthritis. Results showed that the water extract of the stems and roots from VT (VT-SR) was rich in flavones and phenols with 1.1 mg/g of resveratrol, 6.7 mg/g of hopeaphenol, and 5.1 mg/g of (+)-ɛ-viniferin. VT-SR significantly scavenged DPPH radicals and inhibited prostaglandin E2 (PGE2) production in lipopolysaccharide (LPS)-induced PHCs without exhibiting significant cytotoxicity. In in vivo models, the VT-SR (500 mg/kg) significantly decreased serum PGE2 and knee 2-(18)F-fluoro-2-deoxy-D-glucose ((18)F-FDG) levels in LPS-induced acute inflammatory arthritis in rabbits. In addition, dietary supplementation with VT-SR for 28 days significantly alleviated type II collagenase-induced rat osteoarthritis with improvements in weight bearing and range of motion tests. In conclusion, our results suggest that the VT-SR is a good candidate for developing dietary supplements to prevent joint deterioration and inhibit inflammation.
1 Department of Orthopedics, New Taipei City Hospital , New Taipei City, Taiwan .
Ching-Fent
CF
Tsai
Kun-Teng
KT
Wang
Lih-Geeng
LG
Chen
Chia-Jung
CJ
Lee
Sung-Hui
SH
Tseng
Ching-Chiung
CC
Wang
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage* / Q000737:chemistry; D001168:Arthritis / Q000188:drug therapy* / Q000276:immunology; D019902:Chondrocytes / Q000187:drug effects; D015232:Dinoprostone / Q000276:immunology; D006801:Humans; D007719:Knee Joint / Q000187:drug effects / Q000276:immunology*; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D027843:Vitis / Q000737:chemistry*
D000893:Anti-Inflammatory Agents; D010936:Plant Extracts; D015232:Dinoprostone