Endocytic capsule sensors for probing cellular internalization.
A new class of polymer capsules with an in-built endocytic pH-coupled fluorescence switch is reported. These capsules display reversible "on/off" fluorescence in response to cellular pH variations. Using this system, the high-throughput quantification between surface-bound and internalized capsules is demonstrated. This system allows a fundamental study of the interaction between nanoengineered materials and biological systems at a cellular level.
Department of Chemical and Biomolecular Engineering, The University of Melbourne, Parkville, Victoria, 3010, Australia.
Kang
K
Liang
Sylvia T
ST
Gunawan
Joseph J
JJ
Richardson
Georgina K
GK
Such
Jiwei
J
Cui
Frank
F
Caruso
D000818:Animals; D001854:Bone Marrow Cells / Q000737:chemistry / Q000378:metabolism; D002214:Capsules / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics; D002460:Cell Line; D003713:Dendritic Cells / Q000737:chemistry / Q000378:metabolism; D004337:Drug Carriers / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics; D016503:Drug Delivery Systems / Q000295:instrumentation / Q000379:methods*; D004705:Endocytosis / Q000502:physiology*; D005456:Fluorescent Dyes / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics; D006863:Hydrogen-Ion Concentration; D051379:Mice; D008856:Microscopy, Fluorescence; D008958:Models, Molecular; D011109:Polymethacrylic Acids / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics
D002214:Capsules; D004337:Drug Carriers; D005456:Fluorescent Dyes; D011109:Polymethacrylic Acids; C511861:poly(2-(diisopropylamino)ethyl methacrylate)
cell uptake; internalization; layer-by-layer; nanotechnology; polymer capsules; sensors