Chemical investigation of the roots of Polygala sibirica L.
OBJECTIVE
To investigate the chemical constituents of the roots of Polygala sibirica L. (Polygalaceae)


METHODS
The isolation was performed by solvent extraction and various chromatographic techniques, including silica gel, Sephadex LH-20, ODS, semi-preparative HPLC, and preparative TLC. The chemical structures were elucidated based on extensive spectroscopic analysis, including HR-ESI-MS and 1D- and 2D-NMR spectroscopic data.


RESULTS
A total of sixteen compounds, including five xanthones (5, 7-10), five saccharide esters (1, 3, 4, 12, 13), two flavonoids (14, 16), two triterpenoids (11, 15), one phenylpropanoid (6), and one benzophenone glycoside (2) were isolated. Their structures were determined as sibiricose A7 (1), sibiriphenone A (2), polygalatenoside A (3), polygalatenoside C (4), lancerin (5), 3, 4, 5-trimethoxycinnamic acid (6), 6-hydroxy-1, 2, 3, 7-tetramethoxyxanthone (7), 1, 3, 7-trihydroxy-2-methoxyxanthone (8), onjixanthone II (9), 1, 2, 3, 6, 7-pentamethoxyxanthone (10), presenegenin (11), 3'-O-3, 4, 5-trimethoxycinnamoyl-6-O-4-methoxy benzoyl sucrose (12), tenuifoliside C (13), 5, 3'-dihydroxy-7, 4'-dimethoxyflavonol-3-O-β-D-glucopyranoside (14), tenuifolin (15), and rhamnetin 3-O-β-D-glucopyranoside (16).


CONCLUSIONS
Compounds 1 and 2 are two new compounds from P. sibirica.
State Key Laboratory of Natural and Biomimetic Drugs, Peking University Health Science Center, Beijing 100191, China.
Yu-Hong
YH
Zhou
Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China.
Shui-Ying
SY
Zhang
Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China.
Qiang
Q
Guo
Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China.
Xing-Yun
XY
Chai
State Key Laboratory of Natural and Biomimetic Drugs, Peking University Health Science Center, Beijing 100191, China. Electronic address: yongjiang@bjmu.edu.cn.
Yong
Y
Jiang
State Key Laboratory of Natural and Biomimetic Drugs, Peking University Health Science Center, Beijing 100191, China; Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China. Electronic address: pengfeitu@vip.163.com.
Peng-Fei
PF
Tu
D001577:Benzophenones / Q000737:chemistry / Q000302:isolation & purification; D003373:Coumaric Acids / Q000737:chemistry / Q000302:isolation & purification; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D031801:Polygala / Q000737:chemistry; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification; D044004:Xanthones / Q000737:chemistry / Q000302:isolation & purification; D045786:Diterpenes, Kaurane
D001577:Benzophenones; D003373:Coumaric Acids; D005960:Glucosides; D010936:Plant Extracts; D014315:Triterpenes; D044004:Xanthones; C487713:presenegenin; C532370:tenuifolin diterpene; D045786:Diterpenes, Kaurane
Polygala sibirica; Polygalaceae; Sibiricose A7; Sibiriphenone A