The inhibitory effect of vitexin on the agonist-induced regulation of vascular contractility.
The present study was undertaken to investigate the influence of vitexin on vascular smooth muscle contractility and to determine the mechanism involved. Intact or denuded aortic rings from male rats were used and isometric contractions were recorded and combined with molecular experiments. Vitexin more significantly relaxed phorbol ester-induced vascular contraction than thromboxane A2 or fluoride-induced contraction suggesting as a possible anti-hypertensive on the agonist-induced vascular contraction regardless of endothelial nitric oxide synthesis. Furthermore, vitexin significantly inhibited phorbol ester-induced increases in pERK1/2 levels. On the other hand, it did not significantly inhibit thromboxane A2-induced increases in pMYPT1 levels suggesting the mechanism involving the primarily inhibition of MEK activity and the subsequent phosphorylation of ERK1/2. This study provides evidence regarding the mechanism underlying the relaxation effect of vitexin on agonist-induced vascular contraction regardless of endothelial function.
Hyun Gon
HG
Je
Seok Myeong
SM
Hong
Hyun Dong
HD
Je
Uy Dong
UD
Sohn
Yun-Sik
YS
Choi
Seung-Yong
SY
Seo
Young Sil
YS
Min
Su Jin
SJ
Chung
Yong Kyoo
YK
Shin
Tae Jin
TJ
Lee
Eon Sub
ES
Park
Ji Hoon
JH
Jeong
D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects; D047310:Apigenin / Q000494:pharmacology*; D015153:Blotting, Western; D020536:Enzyme Activators / Q000494:pharmacology; D020935:MAP Kinase Signaling System / Q000187:drug effects; D008297:Male; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D009119:Muscle Contraction / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000187:drug effects*; D010703:Phorbol Esters / Q000494:pharmacology; D054645:Protein Phosphatase 1 / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012969:Sodium Fluoride / Q000494:pharmacology; D013928:Thromboxane A2 / Q000494:pharmacology; D054460:rho-Associated Kinases / Q000378:metabolism
D020536:Enzyme Activators; D010703:Phorbol Esters; D013928:Thromboxane A2; D047310:Apigenin; D012969:Sodium Fluoride; C032731:vitexin; D054460:rho-Associated Kinases; D020928:Mitogen-Activated Protein Kinases; C499850:Ppp1r12a protein, rat; D054645:Protein Phosphatase 1