Site-specific cross-linking of collagen peptides by lysyl advanced glycation endproducts.
Cross-linking of proteins by advanced glycation endproducts (AGEs) causes a host of pathological conditions but their exact roles are unknown. Cross-linking lysyl AGEs were synthesized and incorporated into two types of collagen peptides. The utility of these cross-linked peptides for biochemical investigations was demonstrated by proteolysis studies and circular dichroism.
School of Chemical Sciences, The University of Auckland, 23 Symonds Street, Auckland, New Zealand. m.brimble@auckland.ac.nz.
M
M
Kamalov
P W R
PW
Harris
G J S
GJ
Cooper
M A
MA
Brimble
D002851:Chromatography, High Pressure Liquid; D002942:Circular Dichroism; D003094:Collagen / Q000737:chemistry* / Q000378:metabolism; D019281:Dimerization; D017127:Glycation End Products, Advanced / Q000737:chemistry* / Q000378:metabolism; D006037:Glyoxal / Q000737:chemistry; D008239:Lysine / Q000737:chemistry; D011765:Pyruvaldehyde / Q000737:chemistry; D014357:Trypsin / Q000378:metabolism
D017127:Glycation End Products, Advanced; D006037:Glyoxal; D011765:Pyruvaldehyde; D003094:Collagen; D014357:Trypsin; D008239:Lysine