Cross-tissue comparisons of leptin and adiponectin: DNA methylation profiles.
DNA methylation has been mostly studied in circulating blood cells. Although being readily accessible, metabolically active tissues such as adipose tissue would be more informative for the study of metabolic disorders. However, whether or not the blood DNA methylation profile correlates with that of adipose tissue remains unknown. In this study, DNA methylation patterns of variation at LEP and ADIPOQ gene loci were similar between individual CpGs across the different tissues. We also report that DNA methylation levels at biologically relevant CpGs are correlated between blood, subcutaneous, and visceral adipose tissue, and that these nearby CpGs are associated with LEP and ADIPOQ gene expression in adipose tissues. These results will be highly relevant for future epigenetic studies in metabolic disorders.
Department of Biochemistry; Université de Sherbrooke; Sherbrooke, QC Canada ; ECOGENE-21 and Clinical Research Center and Lipid Clinic; Chicoutimi Hospital; Saguenay, QC Canada.
Andrée-Anne
AA
Houde
Department of Biochemistry; Université de Sherbrooke; Sherbrooke, QC Canada ; ECOGENE-21 and Clinical Research Center and Lipid Clinic; Chicoutimi Hospital; Saguenay, QC Canada.
Cécilia
C
Légaré
Department of Surgery; IUCPQ; Quebec, QC Canada.
Frédéric-Simon
FS
Hould
Department of Surgery; IUCPQ; Quebec, QC Canada.
Stéfane
S
Lebel
Department of Surgery; IUCPQ; Quebec, QC Canada.
Picard
P
Marceau
Department of Surgery; IUCPQ; Quebec, QC Canada ; INAF; Université Laval; Quebec, QC Canada ; Axe Endocrinologie et Néphrologie; Centre de Recherche du CHU de Québec; Quebec, QC Canada.
André
A
Tchernof
INAF; Université Laval; Quebec, QC Canada ; Axe Endocrinologie et Néphrologie; Centre de Recherche du CHU de Québec; Quebec, QC Canada.
Marie-Claude
MC
Vohl
Department of Medicine; Division of Endocrinology; Université de Sherbrooke; Sherbrooke, QC Canada ; Department of Population Medicine; Harvard Pilgrim Health Care Institute; Boston, MA USA ; General Medicine Division; Massachusetts General Hospital; Boston, MA USA.
Marie-France
MF
Hivert
Department of Biochemistry; Université de Sherbrooke; Sherbrooke, QC Canada ; ECOGENE-21 and Clinical Research Center and Lipid Clinic; Chicoutimi Hospital; Saguenay, QC Canada.
Luigi
L
Bouchard
blood; epigenetics; subcutaneous adipose tissue; visceral adipose tissue