Current trends in polyhydroxyalkanoates (PHAs) biosynthesis: insights from the recombinant Escherichia coli.
Pursuing the current trend, the "green-polymers", polyhydroxyalkanoates (PHAs) which are degradable and made from renewable sources have been a potential substitute for synthetic plastics. Due to the increasing concern towards escalating crude oil price, depleting petroleum resource and environmental damages done by plastics, PHAs have gained more and more attractions, both from industry and research. From the view point of Escherichia coli, a microorganism that used in the biopolymer large scale production, this paper describes the backgrounds of PHA and summarizes the current advances in PHA developments. In the short-chain-length (scl) PHAs section, the study of poly[(R)-3-hydroxybutyrate] [P(3HB)] as model polymer, ultra-high-molecular-weight P(3HB) which rarely discussed, and P(3HB-co-3HV), another commercialized PHA polymer are included. Other than that, this review also shed some light on the new members of PHA family, lactate-based PHAs and P(3HP) with topics such as block copolymers and invention of novel biopolymers. Flexibility of microorganisms in utilizing different carbon sources to accumulate medium-chain-length (mcl) PHAs and lastly, the promising scl-mcl-PHAs with interesting properties are also discussed.
Department of Chemical and Environmental Engineering, Faculty of Engineering, University of Nottingham Malaysia Campus, Jalan Broga, 43500 Semenyih, Selangor Darul Ehsan, Malaysia.
Yoong Kit
YK
Leong
Department of Chemical and Environmental Engineering, Faculty of Engineering, University of Nottingham Malaysia Campus, Jalan Broga, 43500 Semenyih, Selangor Darul Ehsan, Malaysia; Manufacturing and Industrial Processes Division, Faculty of Engineering, Centre for Food and Bioproduct Processing, University of Nottingham Malaysia Campus, Jalan Broga, 43500 Semenyih, Selangor Darul Ehsan, Malaysia.
Pau Loke
PL
Show
Chemical Engineering, School of Engineering, Monash University, 46150 Bandar Sunway, Selangor Darul Ehsan, Malaysia.
Chien Wei
CW
Ooi
Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Tau Chuan
TC
Ling
Biorefinery & Bioprocess Engineering Laboratory, Department of Chemical Engineering and Material Science, Yuan Ze University, No. 135 Yuan-Tung Road, Chungli, Taoyuan, 320, Taiwan. Electronic address: lanchiwei@saturn.yzu.edu.tw.
John Chi-Wei
JC
Lan
D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D060847:Metabolic Engineering / Q000639:trends*; D054813:Polyhydroxyalkanoates / Q000032:analysis / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
D054813:Polyhydroxyalkanoates; D011994:Recombinant Proteins
Escherichia coli; Metabolic engineering; Polyhydroxyalkanoates