VE-statin/Egfl7 siRNA inhibits angiogenesis in malignant glioma in vitro.
This study investigated the role of VE-statin/Egfl7 and its mechanism in angiogenesis in malignant glioma. Transwell culture plates were used to establish an U251-HUVEC co-culture system, which was used to mimic the interaction between malignant glioma and endothelial cells. Lentiviral vectors expressing VE-statin/Egfl7 siRNA were constructed, and U251 cells and HUVECs were transfected to inhibit VE-statin/Egfl7 expression. The proliferation, adherence, migration, and lumen formation of endothelial cells were assayed to investigate the influence of VE-statin/Egfl7 on angiogenesis in malignant glioma in vitro. Data showed that HUVEC growth was temporarily slowed after silencing the VE-statin/Egfl7 gene but rapidly returned to normal. Although endothelial cell migration was not influenced, cell adherence was markedly inhibited. Furthermore, the endothelial cells failed to generate a capillary-like lumen after VE-statin/Egfl7 gene silencing. Therefore, it can be concluded that VE-statin/Egfl7 may regulate the adherence of endothelial cells, thus playing an important role in endothelium-induced lumen formation during angiogenesis in malignant glioma.
Department of Neurosurgery, First Affiliated Hospital of Jishou University Jishou 416000, Hunan.
Chunhai
C
Huang
Department of Neurosurgery, Xiangya Hospital, Central South University Changsha 410008, Hunan, China ; The Institute of Skull Base Surgery and Neurooncology at Hunan Changsha 410008, Hunan, China.
Xianrui
X
Yuan
Department of Neurosurgery, Xiangya Hospital, Central South University Changsha 410008, Hunan, China ; The Institute of Skull Base Surgery and Neurooncology at Hunan Changsha 410008, Hunan, China.
Zhao
Z
Li
Department of Neurosurgery, First Affiliated Hospital of Jishou University Jishou 416000, Hunan.
Zhi
Z
Tian
Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya Hospital, Central South University Changsha 410008, Hunan, China.
Xianquan
X
Zhan
Department of Neurosurgery, First Affiliated Hospital of Jishou University Jishou 416000, Hunan.
Jingjing
J
Zhang
Department of Neurosurgery, Xiangya Hospital, Central South University Changsha 410008, Hunan, China ; The Institute of Skull Base Surgery and Neurooncology at Hunan Changsha 410008, Hunan, China.
Xuejun
X
Li
D002135:Calcium-Binding Proteins; D002448:Cell Adhesion / Q000502:physiology*; D002478:Cells, Cultured; D018920:Coculture Techniques; D066255:EGF Family of Proteins; D042783:Endothelial Cells / Q000378:metabolism / Q000473:pathology; D016228:Endothelial Growth Factors / Q000235:genetics / Q000378:metabolism*; D005910:Glioma / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D009389:Neovascularization, Pathologic / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D034741:RNA, Small Interfering / Q000235:genetics; D014162:Transfection; D014471:Umbilical Veins
D002135:Calcium-Binding Proteins; D066255:EGF Family of Proteins; C527877:EGFL7 protein, human; D016228:Endothelial Growth Factors; D034741:RNA, Small Interfering
Epidermal growth factor-like domain 7; RNA interference; angiogenesis; malignant glioma