In vitro antiviral activity of circular triple helix forming oligonucleotide RNA towards Feline Infectious Peritonitis virus replication.
Feline Infectious Peritonitis (FIP) is a severe fatal immune-augmented disease in cat population. It is caused by FIP virus (FIPV), a virulent mutant strain of Feline Enteric Coronavirus (FECV). Current treatments and prophylactics are not effective. The in vitro antiviral properties of five circular Triple-Helix Forming Oligonucleotide (TFO) RNAs (TFO1 to TFO5), which target the different regions of virulent feline coronavirus (FCoV) strain FIPV WSU 79-1146 genome, were tested in FIPV-infected Crandell-Rees Feline Kidney (CRFK) cells. RT-qPCR results showed that the circular TFO RNAs, except TFO2, inhibit FIPV replication, where the viral genome copy numbers decreased significantly by 5-fold log10 from 10(14) in the virus-inoculated cells to 10(9) in the circular TFO RNAs-transfected cells. Furthermore, the binding of the circular TFO RNA with the targeted viral genome segment was also confirmed using electrophoretic mobility shift assay. The strength of binding kinetics between the TFO RNAs and their target regions was demonstrated by NanoITC assay. In conclusion, the circular TFOs have the potential to be further developed as antiviral agents against FIPV infection.
Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
Oi Kuan
OK
Choong
Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia ; Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
Parvaneh
0000-0002-8391-9228
P
Mehrbod
Faculty of Science, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
Bimo Ario
BA
Tejo
Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia ; Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
Abdul Rahman
AR
Omar
D000818:Animals; D000998:Antiviral Agents / Q000008:administration & dosage; D002415:Cats; D002460:Cell Line; D016765:Coronavirus, Feline / Q000187:drug effects / Q000235:genetics / Q000472:pathogenicity*; D016766:Feline Infectious Peritonitis / Q000235:genetics* / Q000628:therapy / Q000821:virology; D009841:Oligonucleotides / Q000008:administration & dosage / Q000737:chemistry*; D012313:RNA / Q000008:administration & dosage* / Q000737:chemistry; D000079962:RNA, Circular
D000998:Antiviral Agents; D009841:Oligonucleotides; D000079962:RNA, Circular; D012313:RNA