Protein carbonylation during electron beam irradiation may be responsible for changes in IgE binding to turbot parvalbumin.
The present study aimed to investigate the relationship between protein carbonylation and changes of the IgE reactivity of turbot parvalbumin (PV) following electron beam (EB) irradiation. The concentration of protein carbonyls, specific IgE binding, and IgE binding inhibition between irradiated and oxidized PV were assessed. Irradiation resulted in a 3-fold enhancement in the protein carbonyl content. In purified PV irradiated with a 10-kGy dose, specific IgE binding was reduced by 91.2±6.2%. When raw PV was treated with reactive oxygen species (ROS), the protein carbonyl content increased 17.6-fold, with the specific IgE binding being reduced by 87.9±6.5% at an ROS concentration of 10 nmol/mL. The IgE binding inhibition between irradiated and oxidized PV was investigated using an inhibition ELISA. Results showed that oxidized PV can inhibit the binding between irradiated PV and specific IgE with an IC50 of 8.2-58 ng according to different doses of irradiation. These findings suggest that EB irradiation reduces specific IgE binding, probably by the induction of protein carbonylation.
Food Safety Laboratory, Ocean University of China, Qingdao 266003, PR China. Electronic address: lizhenxing@ouc.edu.cn.
Zhenxing
Z
Li
Food Safety Laboratory, Ocean University of China, Qingdao 266003, PR China.
Zongchao
Z
Lu
Food Safety Laboratory, Ocean University of China, Qingdao 266003, PR China; Microbiological Analytical Centre, PCSIR Labs. Complex, Karachi 75280, Pakistan.
Muhammad Naseem
MN
Khan
Food Safety Laboratory, Ocean University of China, Qingdao 266003, PR China.
Hong
H
Lin
Food Safety Laboratory, Ocean University of China, Qingdao 266003, PR China.
Limin
L
Zhang
D000818:Animals; D004307:Dose-Response Relationship, Radiation; D004797:Enzyme-Linked Immunosorbent Assay; D005296:Ferrous Compounds / Q000494:pharmacology; D005396:Fish Products / Q000009:adverse effects / Q000528:radiation effects*; D029941:Fish Proteins / Q000378:metabolism; D005412:Flatfishes / Q000276:immunology*; D005512:Food Hypersensitivity / Q000276:immunology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007073:Immunoglobulin E / Q000378:metabolism*; D010084:Oxidation-Reduction; D010320:Parvalbumins / Q000276:immunology / Q000378:metabolism*; D050050:Protein Carbonylation / Q000187:drug effects / Q000276:immunology / Q000528:radiation effects*; D017382:Reactive Oxygen Species / Q000378:metabolism
D005296:Ferrous Compounds; D029941:Fish Proteins; D010320:Parvalbumins; D017382:Reactive Oxygen Species; D007073:Immunoglobulin E; D006861:Hydrogen Peroxide; C029451:ferrous chloride
Allergenicity; EB irradiation; Parvalbumin; Protein carbonyls; ROS