Electroporation-based DNA delivery technology: methods for gene electrotransfer to skin.
DNA delivery to for example skin and muscle can easily be performed with electroporation. The method is efficient, feasible, and inexpensive and the future possibilities are numerous. Here we present our protocol for gene transfection to mouse skin using naked plasmid DNA and electric pulses.
Center for Experimental Drug and Gene Electrotransfer (C*EDGE), Department of Oncology, Copenhagen University Hospital Herlev, 75 Herlev Ringvej, 2730, Herlev, Denmark.
Anita
A
Gothelf
Julie
J
Gehl
D000818:Animals; D018274:Electroporation / Q000379:methods*; D015870:Gene Expression; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D051379:Mice; D010957:Plasmids / Q000235:genetics; D012867:Skin / Q000276:immunology / Q000378:metabolism*