Retention-property relationships of 1,2,4-triazoles by micellar and reversed-phase liquid chromatography.
The properties of 21 1,2,4-triazoles, relevant to their pharmacokinetics, were investigated using experimental methods and in silico calculations. The lipophilicities of the compounds were determined experimentally using reversed-phase chromatography and liquid chromatography with micellar mobile phases, or they were calculated in silico from their molecular structures. In the reversed-phase technique, an octadecylsilyl, immobilized artificial membrane and immobilized cholesterol were used as the stationary phases. Biological descriptors of compounds, i.e. log BB, log KHSA, Caco-2, and fu,brain, were determined in silico by using ACD/Percepta software. Principal component analysis was used to indicate similarities between chromatographic, partitioning, and biological variables. Highly significant relationships were determined to exist between the chromatographic parameters and the biological descriptors, especially for log BB and log KHSA. The chromatographic parameters measured on the cholesterol column were recommended for use in predicting the lipophilic and biological properties of the 1,2,4-triazoles that were tested.
Department of Physical Chemistry, Faculty of Chemistry, Maria Curie-Skłodowska University, Lublin, Poland.
Małgorzata
M
Janicka
Anna
A
Pachuta-Stec
D018938:Caco-2 Cells; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D056148:Chromatography, Reverse-Phase / Q000295:instrumentation / Q000379:methods; D006801:Humans; D007700:Kinetics; D008823:Micelles; D014230:Triazoles / Q000097:blood / Q000737:chemistry*
D008823:Micelles; D014230:Triazoles; C045575:1,2,4-triazole
High-performance liquid chromatography; Immobilized artificial membranes; Immobilized cholesterol; Micellar chromatography; Principal component analysis