Rational design of hyperpolarized xenon NMR molecular sensor for the selective and sensitive determination of zinc ions.
Although Zn(2+) ions are involved in large numbers of physiopathological processes, non-invasive detection of Zn(2+) ions in opaque biological samples remains a huge challenge. Here, we developed a novel zinc-responsive hyperpolarized (HP) (129)Xe-based NMR molecular sensor. This HP (129)Xe-based NMR molecular sensor was synthesized by attaching 2-(diphenylphosphino) benzenamine as ligand for zinc ions to the xenon-binding supramolecular cage, cryptophane. The (129)Xe NMR spectroscopy of such molecular sensor was shifted up to 6.4 ppm in the presence of Zn(2+) ions, which was nearly four times larger than that of the reported similar sensor. The application of the sensor would benefit low concentration detection by using indirect NMR/MRI method. The response exhibited high sensitivity and selectivity as discriminated from other six potentially competing metal ions. The application of this sensor in the analysis of zinc ions in the rat serum samples was also evaluated. The strategy is generally applicable in developing sensitive and selective sensors for quantitative determination of zinc ions.
Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, the Chinese Academy of Sciences, Wuhan 430071, PR China.
Ji
J
Zhang
Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, the Chinese Academy of Sciences, Wuhan 430071, PR China.
Weiping
W
Jiang
Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, the Chinese Academy of Sciences, Wuhan 430071, PR China.
Qing
Q
Luo
Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, the Chinese Academy of Sciences, Wuhan 430071, PR China.
Xiaoxiao
X
Zhang
Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, the Chinese Academy of Sciences, Wuhan 430071, PR China.
Qianni
Q
Guo
Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, the Chinese Academy of Sciences, Wuhan 430071, PR China.
Maili
M
Liu
Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, the Chinese Academy of Sciences, Wuhan 430071, PR China. Electronic address: xinzhou@wipm.ac.cn.
Xin
X
Zhou
D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D004867:Equipment Design / Q000592:standards; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D051381:Rats; D014978:Xenon / Q000737:chemistry*; D015032:Zinc / Q000032:analysis*
D014978:Xenon; D015032:Zinc
Cryptophane; Hyperpolarized xenon; Molecular sensor; Nuclear magnetic resonance; Zinc ions