Novel and remarkable enhanced-fluorescence system based on gold nanoclusters for detection of tetracycline.
Tetracycline and Eu(3+), while coexisting, usually appear as a complex by chelating. This complex shows low fluorescence intensity, leading to its limitation of analytical goals. Gold nanoclusters (AuNCs), emerging as novel nano-material, are attracting increasing attentions in multiple fields. Herein, gold nanoclusters first function as a fluorescence-enhanced reagent rather than a conventional fluorescent-probe, and a dramatic enhanced-fluorescence system was built based on Eu(3+)-Tetracycline complex (EuTC) by introducing gold nanoclusters. Simultaneously, three types of gold nanoclusters were employed for exploring various conditions likely affecting the system, which demonstrate that no other gold nanoclusters than DNA-templated gold nanoclusters enormously caused fluorescence-enhancement of EuTC. Moreover, this enhanced-fluorescence system permitted available detection of tetracycline (TC) in a linear range of 0.01-5 μM, with a detection limit of 4 nM at a signal-to-noise ratio of 3. Significantly, the practicality of this method for detection of TC in human urine and milk samples was validated, demonstrating its advantages of simplicity, sensitivity and low cost. Interestingly, this system described here is probably promising for kinds of applications based on its dramatically enhanced-fluorescence.
College of Pharmaceutical Sciences, Southwest University, Chongqing 400716, China. Electronic address: ming4444@swu.edu.cn.
Xiaoming
X
Yang
College of Pharmaceutical Sciences, Southwest University, Chongqing 400716, China.
Shanshan
S
Zhu
College of Pharmaceutical Sciences, Southwest University, Chongqing 400716, China.
Yao
Y
Dou
College of Pharmaceutical Sciences, Southwest University, Chongqing 400716, China.
Yan
Y
Zhuo
College of Pharmaceutical Sciences, Southwest University, Chongqing 400716, China.
Yawen
Y
Luo
College of Pharmaceutical Sciences, Southwest University, Chongqing 400716, China.
Yuanjiao
Y
Feng
D000818:Animals; D002417:Cattle; D005456:Fluorescent Dyes / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000032:analysis; D013050:Spectrometry, Fluorescence / Q000379:methods; D013752:Tetracycline / Q000032:analysis* / Q000097:blood / Q000652:urine
D005456:Fluorescent Dyes; D012710:Serum Albumin, Bovine; D006046:Gold; D013752:Tetracycline
Detection; Enhanced fluorescence; Gold nanoclusters; Tetracycline