Mitochondrial ultrastructure and markers of dynamics in hepatocytes from aged, calorie restricted mice fed with different dietary fats.
In this paper we analyzed changes in hepatocyte mitochondrial mass and ultrastructure as well as in mitochondrial markers of fission/fusion and biogenesis in mice subjected to 40% calorie restriction (CR) for 18 months versus ad libitum-fed controls. Animals subjected to CR were separated into three groups with different dietary fats: soybean oil (also in controls), fish oil and lard. Therefore, the effect of the dietary fat under CR was studied as well. Our results show that CR induced changes in hepatocyte and mitochondrial size, in the volume fraction occupied by mitochondria, and in the number of mitochondria per hepatocyte. Also, mean number of mitochondrial cristae and lengths were significantly higher in all CR groups compared with controls. Finally, CR had no remarkable effects on the expression levels of fission and fusion protein markers. However, considerable differences in many of these parameters were found when comparing the CR groups, supporting the idea that dietary fat plays a relevant role in the modulation of CR effects in aged mice.
Departamento de Biología Celular, Fisiología e Inmunología, Universidad de Córdoba, Campus de Excelencia Internacional Agroalimentario, ceiA3, Córdoba, Spain.
Husam
H
Khraiwesh
Departamento de Biología Celular, Fisiología e Inmunología, Universidad de Córdoba, Campus de Excelencia Internacional Agroalimentario, ceiA3, Córdoba, Spain; VM Molecular Biosciences, University of California, Davis, United States.
José A
JA
López-Domínguez
Departamento de Biología Celular, Fisiología e Inmunología, Universidad de Córdoba, Campus de Excelencia Internacional Agroalimentario, ceiA3, Córdoba, Spain.
Lucía
L
Fernández del Río
Departamento de Biología Celular, Fisiología e Inmunología, Universidad de Córdoba, Campus de Excelencia Internacional Agroalimentario, ceiA3, Córdoba, Spain.
Elena
E
Gutierrez-Casado
Centro Andaluz de Biologia del Desarrollo, Universidad Pablo de Olavide-CSIC, CIBERER, Instituto de Salud Carlos III, Sevilla, Spain.
Guillermo
G
López-Lluch
Centro Andaluz de Biologia del Desarrollo, Universidad Pablo de Olavide-CSIC, CIBERER, Instituto de Salud Carlos III, Sevilla, Spain.
Plácido
P
Navas
Laboratory of Experimental Gerontology, National Institute of Aging, National Institutes of Health, Baltimore, MD, United States.
Rafael
R
de Cabo
VM Molecular Biosciences, University of California, Davis, United States.
Jon J
JJ
Ramsey
Departamento de Biología Celular, Fisiología e Inmunología, Universidad de Córdoba, Campus de Excelencia Internacional Agroalimentario, ceiA3, Córdoba, Spain.
María I
MI
Burón
Departamento de Biología Celular, Fisiología e Inmunología, Universidad de Córdoba, Campus de Excelencia Internacional Agroalimentario, ceiA3, Córdoba, Spain.
José M
JM
Villalba
Departamento de Biología Celular, Fisiología e Inmunología, Universidad de Córdoba, Campus de Excelencia Internacional Agroalimentario, ceiA3, Córdoba, Spain. Electronic address: bc1gorej@uco.es.
José A
JA
González-Reyes
D000367:Age Factors; D000375:Aging / Q000378:metabolism / Q000473:pathology*; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D031204:Caloric Restriction; D048429:Cell Size; D004041:Dietary Fats / Q000008:administration & dosage*; D005395:Fish Oils / Q000008:administration & dosage; D022781:Hepatocytes / Q000378:metabolism / Q000648:ultrastructure*; D008054:Lipid Peroxides / Q000378:metabolism; D008297:Male; D008810:Mice, Inbred C57BL; D008930:Mitochondria, Liver / Q000378:metabolism / Q000648:ultrastructure*; D063154:Mitochondrial Dynamics; D053142:Mitochondrial Size; D063269:Mitochondrial Turnover; D051266:Nuclear Respiratory Factor 1 / Q000378:metabolism; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D013024:Soybean Oil / Q000008:administration & dosage; D013997:Time Factors; D014157:Transcription Factors / Q000378:metabolism
D015415:Biomarkers; D004041:Dietary Fats; D005395:Fish Oils; D008054:Lipid Peroxides; C495634:Nrf1 protein, mouse; D051266:Nuclear Respiratory Factor 1; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; C485290:Ppargc1a protein, mouse; D014157:Transcription Factors; D013024:Soybean Oil; C029310:lard
Calorie restriction; Dietary fat; Hepatocyte; Mature/old-aged mice; Mitochondria