Antioxidant activity of extract and its major constituents from okra seed on rat hepatocytes injured by carbon tetrachloride.
The antioxidant activities and protective effects of total phenolic extracts (TPE) and their major components from okra seeds on oxidative stress induced by carbon tetrachloride (CCl4) in rat hepatocyte cell line were investigated. The major phenolic compounds were identified as quercetin 3-O-glucosyl (1 → 6) glucoside (QDG) and quercetin 3-O-glucoside (QG). TPE, QG, and QDG from okra seeds exhibited excellent reducing power and free radical scavenging capabilities including α, α-diphenyl-β-picrylhydrazyl (DPPH), superoxide anions, and hydroxyl radical. Overall, DPPH radical scavenging activity and reducing power of QG and QDG were higher than those of TPE while superoxide and hydroxyl radical scavenging activities of QG and TPE were higher than those of QDG. Furthermore, TPE, QG, and QDG pretreatments significantly alleviated the cytotoxicity of CCl4 on rat hepatocytes, with attenuated lipid peroxidation, increased SOD and CAT activities, and decreased GPT and GOT activities. The protective effects of TPE and QG on rat hepatocytes were stronger than those of QDG. However, the cytotoxicity of CCl4 on rat hepatocytes was not affected by TPE, QG, and QDG posttreatments. It was suggested that the protective effects of TPE, QG, and QDG on rat hepatocyte against oxidative stress were related to the direct antioxidant capabilities and the induced antioxidant enzymes activities.
College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
Lianmei
L
Hu
College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
Wenlan
W
Yu
College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
Ying
Y
Li
Chemical Engineering Discipline, School of Engineering, Monash University, 46150 Bandar Sunway, Malaysia.
Nagendra
0000-0002-1905-1620
N
Prasad
College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
Zhaoxin
Z
Tang
D028483:Abelmoschus / Q000737:chemistry; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002251:Carbon Tetrachloride / Q000633:toxicity; D022781:Hepatocytes / Q000187:drug effects* / Q000473:pathology; D015227:Lipid Peroxidation / Q000187:drug effects; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry; D051381:Rats; D012639:Seeds / Q000737:chemistry
D000975:Antioxidants; D010936:Plant Extracts; D002251:Carbon Tetrachloride