Immunopotentiating properties of extracellular polysaccharide from Trametes hirsuta strain VKESR.
The effectiveness of extracellular polysaccharides isolated from Trametes hirsuta strain VKESR was evaluated for their immunostimulating properties by in vitro lymphocyte proliferation, phagocytic assay, NK cell activity and cytokine quantification. The splenocyte proliferation assay showed that the extracellular glucans found to have good stimulation index and enhances the NK cell mediated tumor killing. It promotes phagocytosis in treated macrophages in a dose dependent manner. Furthermore, the polysaccharides exhibit significant stimulatory effect on cytokines IL-2 and IFN-γ whereas the polysaccharide has moderate stimulatory effect pro-inflammatory cytokine TNF-α. These immunostimulating properties help in combating various diseases and could be a promising beginning for further research to study the role of extracellular polysaccharide on the host immune systems.
Centre for Advanced Studies in Botany, University of Madras, Chennai, India. Electronic address: shenbhagaraman@gmail.com.
Shenbhagaraman
S
R
Centre for Advanced Studies in Botany, University of Madras, Chennai, India.
Premalatha
P
M K
Centre for Advanced Studies in Botany, University of Madras, Chennai, India.
Jenefar
J
S
Cancer Research Laboratory, Methodist Research Institute, Indiana University Health, Indianapolis, IN, USA.
Jagadish
J
L K
Department of Virology, King Institute of Preventive Medicine, Chennai, India.
Saravanamurali
S
K
Department of Virology, King Institute of Preventive Medicine, Chennai, India.
Kaveri
K
K
Centre for Advanced Studies in Botany, University of Madras, Chennai, India.
Karthik
K
S N
Centre for Advanced Studies in Botany, University of Madras, Chennai, India.
Kaviyarasan
K
V
D000276:Adjuvants, Immunologic / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D016207:Cytokines / Q000096:biosynthesis; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical; D062610:Fungal Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D007694:Killer Cells, Natural / Q000187:drug effects; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000276:immunology; D010587:Phagocytosis / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000166:cytology; D055454:Trametes / Q000737:chemistry*
D000276:Adjuvants, Immunologic; D016207:Cytokines; D062610:Fungal Polysaccharides
Extracellular polysaccharides; Immunostimulation; NK cell activity; Phagocytic assay; Splenocyte proliferation