Establishment of a swine model for validation of perfusion measurement by dynamic contrast-enhanced magnetic resonance imaging.
The aim of the study was to develop a suitable animal model for validating dynamic contrast-enhanced magnetic resonance imaging perfusion measurements. A total of 8 pigs were investigated by DCE-MRI. Perfusion was determined on the hind leg musculature. An ultrasound flow probe placed around the femoral artery provided flow measurements independent of MRI and served as the standard of reference. Images were acquired on a 1.5 T MRI scanner using a 3D T1-weighted gradient-echo sequence. An arterial catheter for local injection was implanted in the femoral artery. Continuous injection of adenosine for vasodilation resulted in steady blood flow levels up to four times the baseline level. In this way, three different stable perfusion levels were induced and measured. A central venous catheter was used for injection of two different types of contrast media. A low-molecular weight contrast medium and a blood pool contrast medium were used. A total of 6 perfusion measurements were performed with a time interval of about 20-25 min without significant differences in the arterial input functions. In conclusion the accuracy of DCE-MRI-based perfusion measurement can be validated by comparison of the integrated perfusion signal of the hind leg musculature with the blood flow values measured with the ultrasound flow probe around the femoral artery.
Radiation and Tumor Clinic, Essen University Hospital, Hufelandstraße. 55, 45147 Essen, Germany.
Anika
0000-0003-0912-7326
A
Sauerbrey
Radiation and Tumor Clinic, Essen University Hospital, Hufelandstraße. 55, 45147 Essen, Germany.
Stefan
S
Hindel
Evangelisches Krankenhaus Wesel GmbH, Schermbecker Landstraße 88, 46485 Wesel, Germany.
Marc
M
Maaß
Central Animal Laboratory, Essen University Hospital, Hufelandstraße. 55, 45147 Essen, Germany.
Christine
C
Krüger
Central Animal Laboratory, Essen University Hospital, Hufelandstraße. 55, 45147 Essen, Germany.
Andreas
A
Wissmann
Department of Small Animal Surgery, Hospital of Veterinary Medicine, Justus Liebig University Giessen, Frankfurter Straße 94, 35392 Gießen, Germany.
Martin
M
Kramer
Charité Universiätsmedizin Berlin, Charitéplatz 1, 10117 Berlin, Germany.
Benno
B
Nafz
Radiation and Tumor Clinic, Essen University Hospital, Hufelandstraße. 55, 45147 Essen, Germany.
Lutz
L
Lüdemann
D000818:Animals; D003287:Contrast Media / Q000737:chemistry; D006801:Humans; D007089:Image Enhancement / Q000379:methods; D008279:Magnetic Resonance Imaging / Q000379:methods*; D023421:Models, Animal; D010477:Perfusion / Q000379:methods*; D012039:Regional Blood Flow; D013552:Swine
D003287:Contrast Media