A new and highly sensitive resonance Rayleigh scattering assay for lysozyme using aptamer-nanogold as a probe.
Gold nanoparticles (GN), 10 nm in size, were modified by using lysozyme aptamer (Apt) to obtain a stable Apt–GN probe in pH 8.05 Tris/HCl buffer solutions containing 0.04 mol/L NaCl. Upon addition of lysozyme (LYS), it reacted with the Apt of the probe to form a very stable Apt–LYS complex and to release GNs, which aggregated to form large clusters with a resonance Rayleigh scattering (RRS) peak at 368 nm. The enhanced peak intensity, ΔI, was linear to the LYS concentration in the range 0.2–5.2 nmol/L, with a detection limit of 0.05 nmol/L. The influence of foreign substance was tested, and the results showed that this RRS method has high selectivity. This Apt–GN RRS method was applied to the analysis of LYS in a real sample, with satisfactory results.
Key Laboratory of Ecology of Rare and Endangered Species and Environmental Conservation of Education Ministry and Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guangxi Normal University, Guilin, 541004, China.
Lu
L
Ma
Xinghui
X
Zhang
Aihui
A
Liang
Qingye
Q
Liu
Zhiliang
Z
Jiang
D052157:Aptamers, Nucleotide / Q000737:chemistry*; D004529:Egg White / Q000737:chemistry; D006046:Gold; D006863:Hydrogen-Ion Concentration; D053768:Metal Nanoparticles / Q000737:chemistry; D015335:Molecular Probes / Q000737:chemistry; D009113:Muramidase / Q000032:analysis* / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity; D012965:Sodium Chloride / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods
D052157:Aptamers, Nucleotide; D015335:Molecular Probes; D012965:Sodium Chloride; D006046:Gold; D009113:Muramidase
aptamer; gold nanoparticle; lysozyme; resonance Rayleigh scattering