Centralspindlin is required for thorax development during Drosophila metamorphosis.
Epithelial morphogenesis is an essential process in all metazoans during both normal development and pathological processes such as wound healing. The coordinated regulation of cell shape, cell size, and cell adhesion during the migration of epithelial sheets ultimately gives rise to the diversity of body plans among different organisms as well as the diversity of cellular structures and tissues within an organism. Metamorphosis of the Drosophila pupa is an excellent system to study these transformative events. During pupal development, the cells of the wing imaginal discs migrate dorsally and fuse to form the adult thorax. Here I show centralspindlin, a protein complex well known for its role in cytokinesis, is essential for migration of wing disc cells and proper thorax closure. I show the subcellular localization of centralspindlin is important for its function in thorax development. This study demonstrates the emerging role of centralspindlin in regulating cell migration and cell adhesion in addition to its previously known function during cytokinesis.
Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, Colorado.
Michael
M
Sfregola
D000818:Animals; D002454:Cell Differentiation; D002455:Cell Division; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D029721:Drosophila Proteins / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000254:growth & development* / Q000378:metabolism; D020690:GTPase-Activating Proteins / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D055785:Gene Knockdown Techniques; D060227:Imaginal Discs / Q000254:growth & development; D008675:Metamorphosis, Biological; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D021381:Protein Transport; D013909:Thorax / Q000254:growth & development* / Q000378:metabolism
D029721:Drosophila Proteins; D020690:GTPase-Activating Proteins; D008869:Microtubule-Associated Proteins; C490516:pav protein, Drosophila; C502951:tum protein, Drosophila
Pavarotti; Tumbleweed; cytokinesis; epithelium; morphogenesis; thorax closure