Development of an aptamer-based impedimetric bioassay using microfluidic system and magnetic separation for protein detection.
An aptamer-based impedimetric bioassay using the microfluidic system and magnetic separation was developed for the sensitive and rapid detection of protein. The microfluidic impedance device was fabricated through integrating the gold interdigitated array microelectrode into a flow cell made of polydimethylsiloxane (PDMS). Aptamer modified magnetic beads were used to capture and separate the target protein, and concentrated into a suitable volume. Then the complexes were injected into the microfluidic flow cell for impedance measurement. To demonstrate the high performance of this novel detection system, thrombin was employed as the target protein. The results showed that the impedance signals at the frequency of 90 kHz have a good linearity with the concentrations of thrombin in a range from 0.1 nM to 10nM and the detection limit is 0.01 nM. Compared with the reported impedimetric aptasensors for thrombin detection, this method possesses several advantages, such as the increasing sensitivity, improving reproducibility, reducing sample volume and assay time. All these demonstrate the proposed detection system is an alternative way to enable sensitive, rapid and specific detection of protein.
College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, PR China.
Yixian
Y
Wang
College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, PR China.
Zunzhong
Z
Ye
College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, PR China.
Jianfeng
J
Ping
College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, PR China.
Shunru
S
Jing
College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, PR China. Electronic address: ybying@zju.edu.cn.
Yibin
Y
Ying
D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation*; D017097:Electric Impedance; D004867:Equipment Design; D006801:Humans; D057230:Limit of Detection; D060328:Magnetic Phenomena; D008839:Microelectrodes; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D013917:Thrombin / Q000032:analysis* / Q000302:isolation & purification
D052157:Aptamers, Nucleotide; D013917:Thrombin
Aptamer; Biosensor; Impedance; Magnetic separation; Microfluidic device; Protein detection