Test-methods on the test-bench: a comparison of complete exhaust and exhaust particle extracts for genotoxicity/mutagenicity assessment.
With the growing number of new exhaust after-treatment systems, fuels and fuel additives for internal combustion engines, efficient and reliable methods for detecting exhaust genotoxicity and mutagenicity are needed to avoid the widespread application of technologies with undesirable effects toward public health. In a commonly used approach, organic extracts of particulates rather than complete exhaust is used for genotoxicity/mutagenicity assessment, which may reduce the reliability of the results. In the present study, we assessed the mutagenicity and the genotoxicity of complete diesel exhaust compared to an organic exhaust particle extract from the same diesel exhaust in a bacterial and a eukaryotic system, that is, a complex human lung cell model. Both, complete exhaust and organic extract were found to act mutagenic/genotoxic, but the amplitudes of the effects differed considerably. Furthermore, our data indicate that the nature of the mutagenicity may not be identical for complete exhaust and particle extracts. Because in addition, differences between the responses of the different biological systems were found, we suggest that a comprehensive assessment of exhaust toxicity is preferably performed with complete exhaust and with biological systems representative for the organisms and organs of interest (i.e., human lungs) and not only with the Ames test.
Adolphe Merkle Institute, University of Fribourg , 1700 Fribourg, Switzerland.
Sandro
S
Steiner
Norbert V
NV
Heeb
Jan
J
Czerwinski
Pierre
P
Comte
Andreas
A
Mayer
Alke
A
Petri-Fink
Barbara
B
Rothen-Rutishauser
D004249:DNA Damage; D020869:Gene Expression Profiling; D006801:Humans; D008168:Lung / Q000187:drug effects; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D052638:Particulate Matter; D015203:Reproducibility of Results; D012486:Salmonella typhimurium / Q000235:genetics; D001335:Vehicle Emissions / Q000633:toxicity*
D009153:Mutagens; D052638:Particulate Matter; D001335:Vehicle Emissions