Cytokine concentration in stored canine erythrocyte concentrates.
OBJECTIVE
To evaluate the effect of leukoreduction (LR) as compared to standard nonleukoreduced (NLR) units on cytokine concentrations in canine erythrocyte concentrates during regular storage time.


METHODS
Randomized, experimental study.


METHODS
University teaching hospital.


METHODS
Ten random-source research dogs.


METHODS
One unit of whole blood was collected from each dog and randomized to standard processing (NLR, n = 5) or prestorage LR (n = 5). All units were stored at 4°C. Samples were aseptically collected from each unit weekly for 5 weeks. On day 35, 1 mL of blood was collected from each unit and submitted for aerobic culture.


RESULTS
An ELISA assay was used to analyze the concentrations of IL-1β, IL-8, TNF-α, and IL-10. There were no significant effects of either group or storage time for IL-1β, IL-10, or TNF-α. IL-8 concentration was significantly increased over the storage period in NLR units, and was significantly higher compared to LR units on days 28 and 35. Aerobic culture was negative on all units.


CONCLUSIONS
This study demonstrated a marked, storage time-dependent accumulation of IL-8 in canine erythrocyte concentrates. Prestorage LR attenuated the accumulation of IL-8. This chemokine may contribute to the proinflammatory effects of transfusion of stored erythrocyte concentrates.
Department of Veterinary Clinical Sciences, College of Veterinary Medicine, University of Illinois at Urbana-Cham-paign, Urbana, IL, 61802.
Raffaella
R
Corsi
Maureen A
MA
McMichael
Stephanie A
SA
Smith
Mauria
M
O'Brien
Jennifer
J
Herring
Thandeka R
TR
Ngwenyama
Alyssa
A
Galligan
Alison N
AN
Beloshapka
Ping
P
Deng
Kelly S
KS
Swanson
D000818:Animals; D001793:Blood Preservation / Q000662:veterinary*; D004285:Dogs / Q000097:blood / Q000502:physiology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D004912:Erythrocytes / Q000502:physiology*; D007378:Interleukins / Q000502:physiology*; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology*
D007378:Interleukins; D014409:Tumor Necrosis Factor-alpha
dogs; inflammation; storage lesion; transfusion