Staphylococcal enterotoxin B suppresses Alix and compromises intestinal epithelial barrier functions.
BACKGROUND
The epithelial barrier dysfunction plays a critical role in the pathogenesis of a broad array of immune diseases. Alix protein is involved in the endolysosome system. This study aims to elucidate the role of Alix in the maintenance of epithelial barrier function.


RESULTS
The results showed that Alix was detected in T84 cells at both mRNA and protein levels. Exposure to Staphylococcal enterotoxin B (SEB) markedly suppressed the expression of Alix in T84 cells, which could be blocked by knocking down the Toll like receptor 2. The exposure to SEB did not affect the TER, but markedly increased the permeability of T84 monolayers to OVA; the OVA passing through T84 monolayers still preserved the antigenicity manifesting inducing antigen specific T cells proliferation.


CONCLUSIONS
Alix protein plays a critical role in the maintenance of the barrier function of T84 monolayers.
Hao
H
Yan
Haitao
H
Yi
Lixin
L
Xia
Zhenke
Z
Zhan
Weiyi
W
He
Jijuan
J
Cao
Ping-Chang
PC
Yang
The first affiliated hospital of Liaoning Medical college, Jinzhou, China. lzg@szu.edu.cn.
Zhigang
Z
Liu
D002135:Calcium-Binding Proteins / Q000096:biosynthesis*; D018797:Cell Cycle Proteins / Q000096:biosynthesis*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D056827:Endosomal Sorting Complexes Required for Transport / Q000096:biosynthesis*; D004768:Enterotoxins / Q000008:administration & dosage*; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007154:Immune System Diseases / Q000378:metabolism / Q000473:pathology*; D007413:Intestinal Mucosa; D008247:Lysosomes / Q000187:drug effects; D051195:Toll-Like Receptor 2 / Q000378:metabolism*
D002135:Calcium-Binding Proteins; D018797:Cell Cycle Proteins; D056827:Endosomal Sorting Complexes Required for Transport; D004768:Enterotoxins; C117020:PDCD6IP protein, human; D051195:Toll-Like Receptor 2; C031818:enterotoxin B, staphylococcal