Ispaghula mucilage-gellan mucoadhesive beads of metformin HCl: development by response surface methodology.
Response surface methodology based on 3(2) factorial design was used to develop ispaghula (Plantago ovata F.) husk mucilage (IHM)-gellan gum (GG) mucoadhesive beads containing metformin HCl through Ca(2+)-ion cross-linked ionotropic-gelation technique for the use in oral drug delivery. GG to IHM ratio and cross-linker (CaCl2) concentration were investigated as independent variables. Drug encapsulation efficiency (DEE, %) and cumulative drug release after 10h (R10h, %) were analyzed as dependent variables. The optimized mucoadhesive beads (F-O) showed DEE of 94.24 ± 4.18%, R10h of 59.13 ± 2.27%. These beads were also characterized by SEM and FTIR analyses. The in vitro drug release from these beads showed controlled-release (zero-order) pattern with super case-II transport mechanism over 10h. The optimized beads showed pH-dependent swelling and good mucoadhesivity with the goat intestinal mucosa. The optimized IHM-GG mucoadhesive beads containing metformin HCl exhibited significant antidiabetic effect in alloxan-induced diabetic rats over 10h.
Department of Pharmaceutics, Seemanta Institute of Pharmaceutical Sciences, Mayurbhanj 757086, Odisha, India. Electronic address: amitkrnayak@yahoo.co.in.
Amit Kumar
AK
Nayak
Department of Pharmaceutical Sciences, Guru Ghasidas Vishwavidyalaya (A Central University), Koni, Bilaspur 495 009, C.G., India.
Dilipkumar
D
Pal
Department of Pharmaceutics, Seemanta Institute of Pharmaceutical Sciences, Mayurbhanj 757086, Odisha, India.
Kousik
K
Santra
D000268:Adhesiveness; D000818:Animals; D017076:Computer-Aided Design; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy; D004337:Drug Carriers / Q000737:chemistry*; D005260:Female; D006863:Hydrogen-Ion Concentration; D008297:Male; D008687:Metformin / Q000737:chemistry* / Q000494:pharmacology / Q000627:therapeutic use; D008863:Microspheres; D009092:Mucous Membrane / Q000737:chemistry*; D062087:Plant Mucilage / Q000737:chemistry*; D010943:Plantago / Q000737:chemistry*; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D051381:Rats
D004337:Drug Carriers; D062087:Plant Mucilage; D011135:Polysaccharides, Bacterial; C048288:gellan gum; D008687:Metformin
Controlled drug release; Gellan gum; Ispaghula husk; Mucilage; Mucoadhesion; Response surface methodology