Furfural and hydroxymethylfurfural tolerance in Escherichia coli ΔacrR regulatory mutants.
The presence of the highly toxic furfural and hydroxymethylfurfural (HMF) in the hydrolysate of lignocellulosic biomass prompted the investigation of the Escherichia coli ΔacrR regulatory mutant for higher tolerance to these compounds, to facilitate the production of biofuels and biochemicals, and further biocatalytic conversions. In comparison with the parental strain, the regulatory mutant with the upregulated efflux pump AcrAB-TolC produced moderately better growth and higher tolerance to concentrations of furfural and HMF between 1 and 2 g L(-1) .
Industrial Biotechnology Program, Institute of Chemical & Engineering Sciences, Agency for Science, Technology and Research (A*STAR), Jurong Island, Singapore.
Annette Lin
AL
Luhe
Chan Yuen
CY
Lim
Henri
H
Gerken
Jinchuan
J
Wu
Hua
H
Zhao
D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics* / Q000502:physiology; D029968:Escherichia coli Proteins / Q000235:genetics*; D005662:Furaldehyde / Q000031:analogs & derivatives* / Q000633:toxicity; D017353:Gene Deletion; D006586:Hexanes / Q000633:toxicity; D012097:Repressor Proteins / Q000172:deficiency / Q000235:genetics*
C515217:AcrR protein, E coli; D029968:Escherichia coli Proteins; D006586:Hexanes; D012097:Repressor Proteins; C008046:5-hydroxymethylfurfural; D005662:Furaldehyde
efflux pump; furfural and HMF tolerance