Microarray-based gene expression profiling to elucidate effectiveness of fermented Codonopsis lanceolata in mice.
In this study, the effect of Codonopsis lanceolata fermented by lactic acid on controlling gene expression levels related to obesity was observed in an oligonucleotide chip microarray. Among 8170 genes, 393 genes were up regulated and 760 genes were down regulated in feeding the fermented C. lanceolata (FCL). Another 374 genes were up regulated and 527 genes down regulated without feeding the sample. The genes were not affected by the FCL sample. It was interesting that among those genes, Chytochrome P450, Dmbt1, LOC76487, and thyroid hormones, etc., were mostly up or down regulated. These genes are more related to lipid synthesis. We could conclude that the FCL possibly controlled the gene expression levels related to lipid synthesis, which resulted in reducing obesity. However, more detailed protein expression experiments should be carried out.
Department of Medical Biomaterials Engineering, Kangwon National University, Chuncheon 200-701, Korea. cwy1012@hanmail.net.
Woon Yong
WY
Choi
Department of Medical Biomaterials Engineering, Kangwon National University, Chuncheon 200-701, Korea. kimjiseon@kangwon.ac.kr.
Ji Seon
JS
Kim
Department of Tourism Food Service Cuisine, Hallym Polytechnic University, Gangwon 200-711, Korea. psjmsj@hanmail.net.
Sung Jin
SJ
Park
Department of Medical Biomaterials Engineering, Kangwon National University, Chuncheon 200-701, Korea. cjma@kangwon.ac.kr.
Choong Je
CJ
Ma
Department of Food Science and Technology, Seowon University, Cheongju, Chungbuk 361-742, Korea. hyeonl@seowon.ac.kr.
Hyeon Yong
HY
Lee
D000818:Animals; D002135:Calcium-Binding Proteins; D029745:Codonopsis / Q000378:metabolism*; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis; D004268:DNA-Binding Proteins; D005285:Fermentation; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D019344:Lactic Acid / Q000737:chemistry; D008055:Lipids / Q000096:biosynthesis; D008099:Liver / Q000201:enzymology / Q000378:metabolism; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009077:Mucins / Q000096:biosynthesis; D009765:Obesity / Q000188:drug therapy* / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000378:metabolism; D013963:Thyroid Hormones / Q000096:biosynthesis; D025521:Tumor Suppressor Proteins
D002135:Calcium-Binding Proteins; D004268:DNA-Binding Proteins; C114202:Dmbt1 protein, mouse; D008055:Lipids; D009077:Mucins; D010936:Plant Extracts; D013963:Thyroid Hormones; D025521:Tumor Suppressor Proteins; D019344:Lactic Acid; D003577:Cytochrome P-450 Enzyme System