CMTM5 exhibits tumor suppressor activity through promoter methylation in oral squamous cell carcinoma.
Oral squamous cell carcinoma (OSCC) is one of the most common types of malignancies in the head and neck region. CKLF-like MARVEL transmembrane domain-containing member 5 (CMTM5) has been recently implicated as a tumor suppressor gene in several cancer types. Herein, we examined the expression and function of CMTM5 in oral squamous cell carcinoma. CMTM5 was down-regulated in oral squamous cell lines and tumor samples from patients with promoter methylation. Treatment with the demethylating agent 5-aza-2'-deoxycytidine restored CMTM5 expression. In the OSCC cell lines CAL27 and GNM, the ectopic expression of CMTM5-v1 strongly inhibited cell proliferation and migration and induced apoptosis. In addition, CMTM5-v1 inhibited tumor formation in vivo. Therefore, CMTM5 might act as a putative tumor suppressor gene through promoter methylation in oral squamous cell carcinoma.
Central Laboratory, Peking University School of Stomatology, Beijing, China.
Heyu
H
Zhang
Center for Human Disease Genomics, Department of Immunology, Key Laboratory of Medical Immunology, Ministry of Health, School of Basic Medical Sciences, Peking University, Beijing, China.
Xu
X
Nan
Central Laboratory, Peking University School of Stomatology, Beijing, China.
Xuefen
X
Li
Department of Oral Pathology, Peking University School of Stomatology, Beijing, China.
Yan
Y
Chen
Department of Oral Pathology, Peking University School of Stomatology, Beijing, China.
Jianyun
J
Zhang
Central Laboratory, Peking University School of Stomatology, Beijing, China.
Lisha
L
Sun
Center for Human Disease Genomics, Department of Immunology, Key Laboratory of Medical Immunology, Ministry of Health, School of Basic Medical Sciences, Peking University, Beijing, China.
Wenlin
W
Han
Department of Oral Pathology, Peking University School of Stomatology, Beijing, China. Electronic address: litiejun22@vip.sina.com.
Tiejun
T
Li
D000328:Adult; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D018925:Chemokines / Q000235:genetics / Q000378:metabolism*; D019175:DNA Methylation; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D062792:MARVEL Domain-Containing Proteins / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000235:genetics* / Q000473:pathology; D011401:Promoter Regions, Genetic; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism*; D055815:Young Adult
C525020:CMTM5 protein, human; D018925:Chemokines; D062792:MARVEL Domain-Containing Proteins; D025521:Tumor Suppressor Proteins
CMTM5; Oral squamous cell carcinoma; Promoter methylation; Tumor suppressor