Selection of a suitable disc bioassay for the screening of anti-tumor molecules.
The crown gall induced in potato discs by Agrobacterium tumefaciens is becoming largely utilised in screening anti-tumor agents. The present work is showing that beet discs are more adequate for the anti-tumor screening test. In fact, maximal tumor induction was observed on beet discs (87.5%), followed by carrot discs (75%) and potato discs (68.5%). Beet discs present the most sensibility to crown gall disease with a fast expression of symptoms and more visible galls without any staining need. The beet discs bioassay was carried out by using some synthesized organometallics known for their antitumor activity in mammalian cells. We found significant crown gall inhibition (20.7% to 40.55%) of the tested compounds. Overall results supported that beet bioassay might be a potential prescreen system of anti-tumor molecules in mammalian cells.
Centre de Biotechnologie de Sfax (Université de Sfax). Route de Sidi Mansour Km 6, BP 1177, 3018 Sfax, Tunisia;
Fatma
F
Trigui
Chimie ParisTech (Ecole Nationale Supérieure de Chimie de Paris), Laboratoire Charles Friedel, UMR CNRS 7223, 11 rue Pierre et Marie Curie, 75231 Paris Cedex 05, France.
Pascal
P
Pigeon
Centre de Biotechnologie de Sfax (Université de Sfax). Route de Sidi Mansour Km 6, BP 1177, 3018 Sfax, Tunisia;
Karim
K
Jalleli
Chimie ParisTech (Ecole Nationale Supérieure de Chimie de Paris), Laboratoire Charles Friedel, UMR CNRS 7223, 11 rue Pierre et Marie Curie, 75231 Paris Cedex 05, France.
Siden
S
Top
Centre de Biotechnologie de Sfax (Université de Sfax). Route de Sidi Mansour Km 6, BP 1177, 3018 Sfax, Tunisia;
Sami
S
Aifa
Centre de Biotechnologie de Sfax (Université de Sfax). Route de Sidi Mansour Km 6, BP 1177, 3018 Sfax, Tunisia; ; Chimie ParisTech (Ecole Nationale Supérieure de Chimie de Paris), Laboratoire Charles Friedel, UMR CNRS 7223, 11 rue Pierre et Marie Curie, 75231 Paris Cedex 05, France.
Mehdi
M
El Arbi
Agrobacterium tumefaciens; antitumor activity; beet discs; bioassay; chemical synthesis; organometalics