Conscious and anaesthetised Göttingen mini-pigs as an in-vivo model for buccal absorption - pH-dependent absorption of metoprolol from bioadhesive tablets.
The potential of buccal mucosa as a site for systemic absorption has attracted increased attention in recent years creating a need for new predictive in-vivo models. The aim of this study was to evaluate anaesthetised and conscious Göttingen mini-pigs as a model for buccal drug absorption by testing pH-dependent absorption of metoprolol from a solid dosage form. Buccal tablets buffered to pH 6.2 and pH 8.9, oral liquid and intravenous injection were tested in four conscious and anaesthetised Göttingen mini-pigs in a non-randomised cross-over study. Blood samples were collected and processed before analysis by ultra-performance liquid chromatography with tandem mass spectrometry detection. An ex-vivo flow retention model was applied to study release and retention of the bioadhesive buccal tablets. The Tmax obtained from the two buccal conscious groups (55 ± 5 and 35 ± 5 min) were significantly different to the buccal anaesthetised groups (120 ± 0 and 165 ± 15 min) for buccal tablet pH 6.2 and pH 8.9, respectively. Also, the absolute bioavailability from the anaesthetised buccal tablet pH 8.9 (20.7 ± 4.0%) had a significant increase compared to all other buccal tablet groups. In conclusion, this study showed a pH-dependent absolute bioavailability of metoprolol when administrated as bioadhesive buccal tablets to anaesthetised mini-pigs. The anaesthesia was found to delay the time to reach maximal plasma concentration of metoprolol as compared to the conscious pig model when administrated as buccal tablets.
Department of Pharmacy, Faculty of Health and Medical Sciences, University of Copenhagen , Copenhagen , Denmark .
Emil
E
Meng-Lund
Jette
J
Jacobsen
Morten B
MB
Andersen
Mads L
ML
Jespersen
Jens-Jacob
JJ
Karlsson
Mats
M
Garmer
Erling B
EB
Jørgensen
René
R
Holm
D000269:Adhesives; D000758:Anesthesia; D000818:Animals; D001682:Biological Availability; D002626:Chemistry, Pharmaceutical; D003243:Consciousness; D018592:Cross-Over Studies; D004304:Dosage Forms; D006863:Hydrogen-Ion Concentration; D007275:Injections, Intravenous; D008297:Male; D008790:Metoprolol / Q000008:administration & dosage* / Q000097:blood / Q000493:pharmacokinetics*; D023421:Models, Animal; D065568:Oral Mucosal Absorption / Q000502:physiology*; D013552:Swine; D013556:Swine, Miniature / Q000378:metabolism*; D013607:Tablets
D000269:Adhesives; D004304:Dosage Forms; D013607:Tablets; D008790:Metoprolol