Application of the BIOMED-2 standardized primer system for the diagnosis of primary gastric B-cell non-Hodgkin's lymphoma.
Primary gastric B-cell non-Hodgkin's lymphoma (B-NHL) has a similar morphocytological presentation to severe chronic gastritis, which complicates the pathological diagnosis of this disease. To investigate the practicality and utility of the BIOMED-2 standardized primer system for the diagnosis of primary gastric B-NHL from endoscopic biopsy specimens, we selected 65 cases of archived paraffin-embedded primary gastric B-NHL specimens as well as 27 cases of severe chronic gastritis samples to serve as a negative control group. The positivity rates of immunoglobulin heavy chain (IgH) gene rearrangements detected by the BIOMED-2 standardized primer system for the two groups were 86.4% and 12.0%, respectively, which are significantly different (p < 0.05). Importantly, the combined detection of the five groups of the IgH primer system increased the detection rate of B-NHL. These findings indicate that the BIOMED-2 standardized primer system is suitable for formalin-fixed and paraffin-embedded (FFPE) specimens and is valuable as a secondary diagnostic tool for primary gastric B-NHL as well as the differential diagnosis of severe chronic gastritis.
Department of Pathology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Cong-Wei
CW
Jia
Jian
J
Sun
Yu-Mei
YM
Gu
Ting-Ting
TT
Zhang
Ming-Yang
MY
Liu
Jie
J
Chen
Zhao-Hui
ZH
Lu
D000293:Adolescent; D000328:Adult; D000368:Aged; D001706:Biopsy; D017931:DNA Primers; D016099:Endoscopy, Gastrointestinal; D005260:Female; D005756:Gastritis / Q000175:diagnosis*; D016480:Helicobacter pylori / Q000302:isolation & purification; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D016393:Lymphoma, B-Cell / Q000175:diagnosis*; D008228:Lymphoma, Non-Hodgkin / Q000175:diagnosis*; D008297:Male; D008875:Middle Aged; D016612:Paraffin Embedding; D013274:Stomach Neoplasms / Q000175:diagnosis*; D055815:Young Adult
D017931:DNA Primers; D007143:Immunoglobulin Heavy Chains
BIOMED-2; diagnosis; gene rearrangement; immunoglobulin heavy chain; primary gastric B-NHL