In vitro and in vivo anti-Helicobacter pylori activities of FEMY-R7 composed of fucoidan and evening primrose extract.
Effects of FEMY-R7, composed of fucoidan and evening primrose extract, on the bacterial growth and intragastric infection of Helicobacter pylori as well as gastric secretion were investigated in comparison with a proton-pump inhibitor pantoprazole. For in vitro anti-bacterial activity test, H. pylori (1×10(8) CFU/mL) was incubated with a serially-diluted FEMY-R7 for 3 days. As a result, FEMY-R7 fully inhibited the bacterial growth at 100 µg/mL, which was determined to be a minimal inhibitory concentration. In addition, 6-hour incubation with H. pylori, FEMY-R7 inhibited urease activity in a concentration-dependent manner, showing a median inhibitory concentration of 1,500 µg/mL. In vivo elimination study, male C57BL/6 mice were infected with the bacteria by intragastric inoculation (5×10(9) CFU/mouse) 3 times at 2-day intervals, and simultaneously, orally treated twice a day with 10, 30 or 100 mg/kg FEMY-R7 for 7 days. In Campylobcter-like organism-detection test and bacterial identification, FEMY-R7 exerted a high bacteria-eliminating capacity at 30-100 mg/kg, comparably to 30 mg/kg pantoprazole. In contrast to a strong antacid activity of pantoprazole in a pylorus-ligation study, FEMY-R7 did not significantly affect gastric pH, free HCl, and total acidity, although it significantly decreased fluid volume at a low dose (10 mg/kg). The results indicate that FEMY-R7 eliminate H. pylori from gastric mucosa by directly killing the bacteria and preventing their adhesion and invasion, rather than by inhibiting gastric secretion or mucosal damage.
College of Veterinary Medicine, Chungbuk National University, Cheongju, Korea.
Jingmei
J
Cai
Misuba RTech Co., Ltd., Asan, Korea.
Tae-Su
TS
Kim
College of Veterinary Medicine, Chungbuk National University, Cheongju, Korea.
Ja Young
JY
Jang
College of Veterinary Medicine, Chungbuk National University, Cheongju, Korea.
Jihyun
J
Kim
College of Veterinary Medicine, Chungbuk National University, Cheongju, Korea.
Kyungha
K
Shin
Misuba RTech Co., Ltd., Asan, Korea.
Sung-Pyo
SP
Lee
College of Veterinary Medicine, Chungbuk National University, Cheongju, Korea.
Ehn-Kyoung
EK
Choi
Department of Biomedical Laboratory Sciences, Yonsei University, Wonju, Korea.
Sa-Hyun
SH
Kim
Department of Biomedical Laboratory Sciences, Yonsei University, Wonju, Korea.
Min
M
Park
Department of Biomedical Laboratory Sciences, Yonsei University, Wonju, Korea.
Jong Bae
JB
Kim
College of Veterinary Medicine, Chungbuk National University, Cheongju, Korea.
Yun-Bae
YB
Kim
CLO test; FEMY-R7; Helicobacter pylori; evening primrose extract; fucoidan; gastric secretion; minimal inhibitory concentration (MIC)