Temperature dependence of photosynthesis and thylakoid lipid composition in the red snow alga Chlamydomonas cf. nivalis (Chlorophyceae).
Here, we report an effect of short acclimation to a wide span of temperatures on photosynthetic electron transfer, lipid and fatty acid composition in the snow alga Chlamydomonas cf. nivalis. The growth and oxygen evolution capacity were low at 2 °C yet progressively enhanced at 10 °C and were significantly higher at temperatures from 5 to 15 °C in comparison with the mesophilic control Chlamydomonas reinhardtii. In search of the molecular mechanisms responsible for the adaptation of photosynthesis to low temperatures, we have found unprecedented high rates of QA to QB electron transfer. The thermodynamics of the process revealed the existence of an increased structural flexibility that we explain with the amino acid changes in the D1 protein combined with the physico-chemical characteristics of the thylakoid membrane composed of > 80% negatively charged phosphatidylglycerol.
Faculty of Science, University of South Bohemia in České Budějovice, České Budějovice, Czech Republic; Department of Phototrophic Microorganisms, Institute of Microbiology CAS, Opatovický mlýn, Třeboň, Czech Republic.
Martin
M
Lukeš
Lenka
L
Procházková
Volha
V
Shmidt
Linda
L
Nedbalová
David
D
Kaftan
D000222:Adaptation, Physiological; D000595:Amino Acid Sequence; D002696:Chlamydomonas / Q000254:growth & development / Q000378:metabolism*; D003080:Cold Temperature; D017124:Conserved Sequence; D004579:Electron Transport; D007425:Intracellular Membranes / Q000378:metabolism; D050356:Lipid Metabolism; D008055:Lipids; D008969:Molecular Sequence Data; D010100:Oxygen / Q000378:metabolism; D010788:Photosynthesis; D045332:Photosystem II Protein Complex / Q000737:chemistry / Q000378:metabolism*; D011809:Quinones / Q000378:metabolism; D013696:Temperature; D013816:Thermodynamics; D020524:Thylakoids / Q000378:metabolism
D008055:Lipids; D045332:Photosystem II Protein Complex; D011809:Quinones; D010100:Oxygen
Chlamydomonas; electron transfer; phosphatidylglycerol; photosystem II; snow algae; temperature adaptation