Bactericidal activity of elastin-like polypeptide biopolymer with polyhistidine domain and silver.
In the present study, elastin-like biopolymer (ELP) composed of a polyhistidine domain has been investigated as a silver binding agent for antibacterial activity against Escherichia coli, a model test strain for Gram-negative bacteria for antibacterial assays of nanoparticles, and Vibrio harveyi, an opportunistic pathogen which cause mass mortality in shrimp Penaeus monodon reared in coastal aquaculture. The concentration dependent antimicrobial activity of ELPH-Ag on E. coli and V. harveyi was examined by agar well diffusion method and further confirmed through growth curves using spectrophotometer assisted absorption observations. The increased concentrations of ELP-Ag effectively checked the bacterial growth and increased the diameter of inhibition zone. The results showed a minimum inhibitory concentration of 37 μg/ml. This study has an application in formulating artificial protein based antibacterial in diverse fields of healthcare and management of disease in coastal aquaculture.
Department of Chemical and Environmental Engineering, University of California, Riverside, CA, USA.
Kishore K
KK
Krishnani
Department of Chemical and Environmental Engineering, University of California, Riverside, CA, USA; Department of Chemical and Biomolecular Engineering, University of Delaware, DE, USA.
Wilfred
W
Chen
Department of Chemical and Environmental Engineering, University of California, Riverside, CA, USA. Electronic address: adani@engr.ucr.edu.
Ashok
A
Mulchandani
D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000633:toxicity*; D058491:Bacterial Load; D004549:Elastin / Q000737:chemistry*; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development; D006639:Histidine / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry / Q000633:toxicity; D008826:Microbial Sensitivity Tests; D012834:Silver / Q000737:chemistry*; D014733:Vibrio / Q000187:drug effects* / Q000254:growth & development
D000900:Anti-Bacterial Agents; C033223:polyhistidine; D012834:Silver; D006639:Histidine; D004549:Elastin
Aquaculture; Bactericidal activity; Biopolymer; Health care; Silver binding