Analysis of gingival microvessels ultrastructure in the animal model study.
OBJECTIVE
The aim of this study was to investigate ultrastructural changes that occur in gingival microvessels on acidotic model of periodontitis in rats.


METHODS
Gingival tissue samples were obtained from 80 mongrel male rats, from which 16 represented the negative control group, 16 the positive control group and the rest of 48 the three study groups with acid-induced periodontitis. Specimens were fixed in 1.5% osmium tetroxide solution and examined (×4000-×6000) in transmission electron microscope JEM-100 CX II (JEOL Ltd., Japan) with an embedded AMT Digital Camera.


RESULTS
In study groups, substantially reduced vascular permeability, preserved mitochondrial cristae and enhanced microvesiculation in endothelial cells, condensed basement membranes were observed at the closing stage of experiment that contributed to the significantly lower severity of gingival inflammation on visual examination than in positive controls.


CONCLUSIONS
Our data suggest that the key role in the recovery of endothelial and perivascular integrity is attributed to the stabilization of vascular cells membranes in rats gingiva as the result of protein and lipid metabolism regulation.
Department of Therapeutic Stomatology, Faculty of Postgraduate Education, Danylo Halytskyi Lviv National Medical University, Lviv, Ukraine; kordiyakolena68@gmail.com.
Olena J
OJ
Kordiyak
Zoryana Z
ZZ
Masna
D000818:Animals; D002196:Capillaries / Q000648:ultrastructure; D004903:Erythrocyte Aggregation; D004912:Erythrocytes / Q000648:ultrastructure; D005881:Gingiva / Q000098:blood supply*; D008297:Male; D055806:Microvessels / Q000648:ultrastructure*; D023421:Models, Animal; D020286:Pericytes / Q000648:ultrastructure; D051381:Rats