Liquid chromatography-tandem mass spectrometry method for toxicokinetics, tissue distribution, and excretion studies of T-2 toxin and its major metabolites in pigs.
A rapid and sensitive high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for quantitatively analyzing T-2 toxin and its major metabolites (HT-2 toxin and T-2 triol) in swine biological samples. For all matrices, liquid-liquid extraction (ethyl acetate or acetonitrile) and Varian Bond-Elut MycoSep cartridges for solid phase extraction were used for sample preparation. The analytes were separated via a Zorbax XDB-C18 column and were detected using LC-MS/MS with an electrospray ionization interface in positive ion mode. The resulting calibration curves offered satisfactory linearity (r(2)>0.992) within the test range. The limits of quantification for T-2 toxin, HT-2 toxin, and T-2 triol were 1ng/mL (μg/kg), 2ng/mL (μg/kg), and 5ng/mL (μg/kg), respectively. The recovery rates in different matrices ranged from 74.3% to 102.4%, and the interday and intraday precisions were all less than 10.2% for the target analytes. The developed method was successfully applied to toxicokinetics, tissue distribution, and excretion studies of T-2 toxin and its major metabolites after intravenous (i.v.) administration in pigs. The results provide important information for evaluating and controlling human exposure to residual T-2 toxin and its major metabolites in animal-derived food.
Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
Yongxue
Y
Sun
Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
Guijun
G
Zhang
Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
Haiyan
H
Zhao
Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
Jianlong
J
Zheng
Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
Fangyuan
F
Hu
Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China. Electronic address: fangbh@scau.edu.cn.
Binghu
B
Fang
D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods; D057230:Limit of Detection; D059625:Liquid-Liquid Extraction; D009183:Mycotoxins / Q000008:administration & dosage / Q000378:metabolism* / Q000493:pharmacokinetics*; D013552:Swine; D013605:T-2 Toxin / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000378:metabolism* / Q000493:pharmacokinetics*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014018:Tissue Distribution; D066007:Toxicokinetics
D009183:Mycotoxins; C038854:scirpentriol; D013605:T-2 Toxin; C012351:HT-2 toxin
Excretion; LC–MS/MS; Pigs; T-2 toxin; Tissue distribution; Toxicokinetics