Comparison of monolithic capillary electrochromatography and micellar electrokinetic chromatography for the separation of polycyclic aromatic hydrocarbons.
Atmospheric pollution of anthropic origin is recognized as a major risk factor for health, in particular for respiratory and cardio-vascular systems. Among these pollutants, polycyclic aromatic hydrocarbons (PAHs) are placed on the list of US Environmental Protection Agency (EPA) as 'priority' pollutants and four of them are assigned as potential carcinogens by The International Agency for Research on Cancer (IARC). In the present work two capillary techniques-micellar electrokinetic chromatography (MEKC) and monolithic capillary electrochromatography (CEC)-were compared for the separation of eleven PAHs. Both techniques compared in the present work are fully compatible with every standard apparatus of capillary electrophoresis. For MEKC, enhancement of selectivity and decrease of the separation window of eleven PAHs were obtained with methanol:borate 25 mM (20/80, v/v) running buffer containing 10 mM of hydroxypropylated γ-cyclodextrins with low SDS content (25 mM). In case of CEC, two acrylate-based monolithic stationary phases (MSPs) were evaluated for their application in the separation of eleven PAHs. The best MSP based on butyl acrylate was compared with MEKC in terms of sample capacity, PAHs elution order, LOQ, efficiency and effect of pH. Influence of the hydrophobicity of mobile phase on the PAHs elution order was also studied.
Univ. Orléans, CNRS, ICOA, UMR-7311, F-45067 Orléans, France.
Aleksander
A
Salwiński
Normandie Univ, France; UNICAEN, ABTE EA4651, F-14032 Caen, France; Centre François Baclesse, F-14076 Caen, France. Electronic address: Raphael.Delepee@unicaen.fr.
Raphaël
R
Delépée
D053801:Capillary Electrochromatography / Q000379:methods / Q000592:standards*; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods / Q000592:standards*; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis* / Q000737:chemistry
D011084:Polycyclic Aromatic Hydrocarbons
Micellar electrokinetic chromatography; Monolithic capillary electrochromatography; Polycyclic aromatic hydrocarbons