Functional effect of local administration of glial derived neurotrophic factor combined with inside-out artery graft on sciatic nerve regeneration in rat.
Effect of local glial derived neurotrophic factor (GDNF) on nerve regeneration was assessed. Eighty male Wistar rats were divided into four experimental groups (n = 20), randomly: In transected group left sciatic nerve was transected and stumps were fixed in adjacent muscle. In treatment group defect was bridged using an artery graft filled with 10 μL GDNF. In artery group graft was filled with phosphate-buffered saline. In normal control group sciatic nerve was exposed and manipulated. Each group was subdivided into four subgroups of five animals each and nerve fibers were studied in a 16-week period. Behavioral, functional, electrophysiological and gastrocnemius muscle mass findings and morphometric indices confirmed faster recovery of regenerated axons in IOAG/GDNF than in IOAG group (p < 0.05). Immunohistochemical reactions to S-100 in IOAG/GDNF were more positive than that in IOAG group. GDNF improved functional recovery and morphometric indices of sciatic nerve. It could be considered as an effective treatment for peripheral nerve repair in practice.
Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia University, Nazloo Road, Urmia 57153 1177, Iran.
Nooshin
N
Ghayemi
Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia 57159 44867, Iran.
Amin
A
Haghighat
Department of Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan S7N 5B4, Canada.
Keyvan
K
Amini
Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia University, Nazloo Road, Urmia 57153 1177, Iran. Electronic address: r.mohammadivet@gmail.com.
Rahim
R
Mohammadi
D000818:Animals; D001158:Arteries / Q000737:chemistry / Q000637:transplantation*; D001522:Behavior, Animal / Q000187:drug effects; D004576:Electromyography; D051100:Glial Cell Line-Derived Neurotrophic Factor / Q000494:pharmacology*; D008297:Male; D018482:Muscle, Skeletal / Q000737:chemistry / Q000187:drug effects / Q000502:physiology; D009416:Nerve Regeneration / Q000187:drug effects*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D020127:Recovery of Function / Q000187:drug effects; D009418:S100 Proteins / Q000378:metabolism; D012584:Sciatic Nerve / Q000187:drug effects* / Q000502:physiology / Q000601:surgery
D051100:Glial Cell Line-Derived Neurotrophic Factor; D009418:S100 Proteins
GDNF; Local; Peripheral nerve repair; Rat; Sciatic