Recommended minimal standards for description of new taxa of the genera Bifidobacterium, Lactobacillus and related genera.
Minimal standards for the description of new cultivable strains that represent novel genera and species belonging to the genera Bifidobacterium, Lactobacillus and related genera are proposed in accordance with Recommendation 30b of the Bacteriological Code (1990 Revision): the description of novel species should be based on phenotypic, genotypic and ecological characteristics to ensure a rich polyphasic characterization. Concerning genotypic characterization, in addition to DNA G+C content (mol%) data, the description should be based on DNA-DNA hybridization (DDH), 16S rRNA gene sequence similarities and at least two housekeeping gene (e.g. hsp60 and recA) sequence similarities. DDH might not be needed if the 16S rRNA gene sequence similarity to the closest known species is lower than 97 %. This proposal has been endorsed by members of the Subcommittee on the Taxonomy of Bifidobacterium, Lactobacillus and related organisms of the International Committee on the Systematics of Prokaryotes.
Department of Agricultural Sciences, University of Bologna, Viale Fanin 42, 40127 Bologna, Italy.
Paola
P
Mattarelli
School of Life Sciences, Handong Global University, Pohang, Gyeongbuk 791-798, Republic of Korea.
Wilhelm
W
Holzapfel
Max Rubner-Institut, Federal Research Institute for Nutrition and Food, Department of Safety and Quality of Fruit and Vegetables, Haid-und-Neu-Strasse 9, D-76131 Karlsruhe, Germany.
Charles M A P
CMAP
Franz
Department of Food and Cosmetic Science, Faculty of Bioindustry, Tokyo University of Agriculture, 099-2493 Hokkaido, Japan.
Akihito
A
Endo
Department of Biotechnology, University of Verona, Strada Le Grazie 15, 37134 Verona, Italy.
Giovanna E
GE
Felis
Talstr. 60/1, D-70794 Filderstadt, Germany.
Walter
W
Hammes
Institut Pasteur de Lille, Lactic acid Bacteria & Mucosal Immunity, Center for Infection and Immunity of Lille, 1 rue du Pr Calmette, BP 245, F-59019 Lille, France.
Bruno
B
Pot
Department of Microbiology, University of Stellenbosch, 7600 Stellenbosch, South Africa.
Leon
L
Dicks
Department of Biotechnology, University of Verona, Strada Le Grazie 15, 37134 Verona, Italy.
Franco
F
Dellaglio
D015373:Bacterial Typing Techniques / Q000592:standards*; D001482:Base Composition; D001644:Bifidobacterium / Q000145:classification*; D018834:Chaperonin 60 / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D005838:Genotype; D007778:Lactobacillus / Q000145:classification*; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D011938:Rec A Recombinases / Q000235:genetics
D018834:Chaperonin 60; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D011938:Rec A Recombinases