Bone marrow mesenchymal stem cell-derived microvesicles protect rat pheochromocytoma PC12 cells from glutamate-induced injury via a PI3K/Akt dependent pathway.
Studies have suggested that mesenchymal stem cells (MSCs) can protect neuronal cells from excitotoxicity, but the underlying mechanisms are still remaining elusive. In the study, we show that microvesicles released by rat bone marrow-derived MSCs (rBMSC-MVs) protect rat pheochromocytoma PC12 cells from glutamate-induced excitotoxicity. BMSC-MVs upregulate Akt phosphorylation and Bcl-2 expression, downregulate Bax expression, and reduce the cleavage of caspase-3 in glutamate-treated PC12 cells. Such protective effects are partially abrogated by inhibiting PI3K, indicating that rBMSC-MVs act via the PI3K/Akt pathway. Transplantation of rBMSC-MVs may, therefore, be a promising strategy to treat cerebral injury or some other neuronal diseases involving excitotoxicity.
Department of Rehabilitation Medicine, Zhujiang Hospital, Southern Medical University, 253 Gongye Road, Guangzhou, 510282, China.
Shan-Shan
SS
Lin
Bo
B
Zhu
Zi-Kuan
ZK
Guo
Guo-Zhi
GZ
Huang
Zi
Z
Wang
Jin
J
Chen
Xiao-Juan
XJ
Wei
Qi
Q
Li
D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism; D005971:Glutamates / Q000633:toxicity*; D008297:Male; D059630:Mesenchymal Stem Cells; D016716:PC12 Cells; D010766:Phosphorylation; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D022161:Transport Vesicles / Q000502:physiology*
D005971:Glutamates; D051057:Proto-Oncogene Proteins c-akt