Microfluidic perfusion cell culture system confined in 35 mm culture dish for standard biological laboratories.
An extremely simple, self-standing microfluidic cell culture system is reported. The whole system is confined in a 35 mm culture dish, and requires only a standard CO2 incubator. The culture medium is perfused by gravity. We successfully cultured NIH3T3-derived cells up to 10 days with a viability of ∼90%.
Bioengineering Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan; Department of Advanced Materials Science, University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba 227-8561, Japan.
Eitaro
E
Kondo
Bioengineering Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
Ken-Ichi
K
Wada
Bioengineering Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan. Electronic address: k-hoso@riken.go.jp.
Kazuo
K
Hosokawa
Bioengineering Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan; Department of Advanced Materials Science, University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba 227-8561, Japan.
Mizuo
M
Maeda
D000818:Animals; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000592:standards; D049109:Cell Proliferation; D003470:Culture Media; D051379:Mice; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000592:standards; D041681:NIH 3T3 Cells; D010477:Perfusion / Q000379:methods
D003470:Culture Media
Cell culture; Gravity; Microfluidics; NIH3T3; Perfusion