Structure and antimicrobial activity of platypus 'intermediate' defensin-like peptide.
The three-dimensional structure of a chemically synthesized peptide that we have called 'intermediate' defensin-like peptide (Int-DLP), from the platypus genome, was determined by nuclear magnetic resonance (NMR) spectroscopy; and its antimicrobial activity was investigated. The overall structural fold of Int-DLP was similar to that of the DLPs and β-defensins, however the presence of a third antiparallel β-strand makes its structure more similar to the β-defensins than the DLPs. Int-DLP displayed potent antimicrobial activity against Staphylococcus aureus and Pseudomonas aeruginosa. The four arginine residues at the N-terminus of Int-DLP did not affect the overall fold, but were important for its antimicrobial potency.
Nanoscale Organisation and Dynamics Group, School of Science and Health, University of Western Sydney, Penrith, NSW 2751, Australia. Electronic address: a.torres@uws.edu.au.
Allan M
AM
Torres
Queensland Tropical Health Alliance, James Cook University, Cairns, Qld 4878, Australia.
Paramjit
P
Bansal
Neurotoxin Research Group, School of Medical & Molecular Biosciences, University of Technology, Sydney, NSW 2007, Australia.
Jennifer M S
JM
Koh
Singapore Bioimaging Consortium, Biomedical Sciences Institutes, A*STAR, Singapore 138667, Singapore.
Guilhem
G
Pagès
Nanoscale Organisation and Dynamics Group, School of Science and Health, University of Western Sydney, Penrith, NSW 2751, Australia.
Ming J
MJ
Wu
School of Molecular Bioscience, University of Sydney, NSW 2006, Australia.
Philip W
PW
Kuchel
D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D023082:Defensins / Q000737:chemistry* / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D010987:Platypus; D017433:Protein Structure, Secondary; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects
D000900:Anti-Bacterial Agents; D023082:Defensins
Defensin like peptide; Intermediate-DLP; NMR spectroscopy; Peptide fold; Platypus; β-Defensin