Prevention of dentine erosion by brushing with anti-erosive toothpastes.
OBJECTIVE
This in vitro study aimed to investigate the preventive effect of brushing with anti-erosive toothpastes compared to a conventional fluoride toothpaste on dentine erosion.


METHODS
Bovine dentine specimens (n=12 per subgroup) were eroded in an artificial mouth (6 days, 6×30 s/day) using either citric acid (pH:2.5) or a hydrochloric acid/pepsin solution (pH:1.6), simulating extrinsic or intrinsic erosive conditions, respectively. In between, the specimens were rinsed with artificial saliva. Twice daily, the specimens were brushed for 15 s in an automatic brushing machine at 2.5 N with a conventional fluoride toothpaste slurry (elmex, AmF) or toothpaste slurries with anti-erosive formulations: Apacare (NaF/1% nHAP), Biorepair (ZnCO3-HAP), Chitodent (Chitosan), elmex Erosionsschutz (NaF/AmF/SnCl2/Chitosan), mirasensitive hap (NaF/30% HAP), Sensodyne Proschmelz (NaF/KNO3). Unbrushed specimens served as control. Dentine loss was measured profilometrically and statistically analysed using two-way and one-way ANOVA followed by Scheffe's post hoc tests. RDA-values of all toothpastes were determined, and linear mixed models were applied to analyse the influence of toothpaste abrasivity on dentine wear (p<0.05).


RESULTS
Dentine erosion of unbrushed specimens amounted to 5.1±1.0 μm (extrinsic conditions) and 12.9±1.4 μm (intrinsic conditions). All toothpastes significantly reduced dentine erosion by 24-67% (extrinsic conditions) and 21-40% (intrinsic conditions). Biorepair was least effective, while all other toothpastes were not significantly different from each other. Linear mixed models did not show a significant effect of the RDA-value of the respective toothpaste on dentine loss.


CONCLUSIONS
Toothpastes with anti-erosive formulations reduced dentine erosion, especially under simulated extrinsic erosive conditions, but were not superior to a conventional fluoride toothpaste.
Department of Preventive Dentistry, Periodontology and Cariology, University of Zurich, Switzerland; Department of Pedodontics, Faculty of Dentistry, University of Ege, Izmir, Turkey. Electronic address: a_aykut@hotmail.com.
Arzu
A
Aykut-Yetkiner
Department of Preventive Dentistry, Periodontology and Cariology, University of Zurich, Switzerland.
Thomas
T
Attin
Department for Preventive Dentistry, Periodontology and Cariology, University of Göttingen, Germany.
Annette
A
Wiegand
D000818:Animals; D002254:Carbonates / Q000627:therapeutic use; D002327:Cariostatic Agents / Q000627:therapeutic use; D002417:Cattle; D048271:Chitosan / Q000627:therapeutic use; D019343:Citric Acid / Q000009:adverse effects; D003804:Dentin / Q000187:drug effects* / Q000473:pathology; D003959:Diamines / Q000627:therapeutic use; D004338:Drug Combinations; D017886:Durapatite / Q000627:therapeutic use; D005459:Fluorides / Q000627:therapeutic use; D006851:Hydrochloric Acid / Q000009:adverse effects; D008422:Materials Testing; D009566:Nitrates / Q000627:therapeutic use; D010434:Pepsin A / Q000009:adverse effects; D010710:Phosphates / Q000627:therapeutic use; D020011:Protective Agents / Q000627:therapeutic use; D012464:Saliva, Artificial / Q000737:chemistry; D012969:Sodium Fluoride / Q000627:therapeutic use; D017971:Tin Compounds / Q000627:therapeutic use; D014077:Tooth Erosion / Q000473:pathology / Q000517:prevention & control*; D014100:Toothpastes / Q000627:therapeutic use*; D017967:Zinc Compounds / Q000627:therapeutic use
D002254:Carbonates; D002327:Cariostatic Agents; D003959:Diamines; D004338:Drug Combinations; D009566:Nitrates; D010710:Phosphates; D020011:Protective Agents; D012464:Saliva, Artificial; C097427:Sensodyne; D017971:Tin Compounds; D014100:Toothpastes; D017967:Zinc Compounds; C023599:stannous chloride; D019343:Citric Acid; C495399:Olaflur; D012969:Sodium Fluoride; D048271:Chitosan; D017886:Durapatite; D010434:Pepsin A; C036650:zinc carbonate; D005459:Fluorides; D006851:Hydrochloric Acid
Dentine; Erosion; Toothpaste