Immunoregulatory effects of bone marrow-derived mesenchymal stem cells in the nasal polyp microenvironment.
Nasal polyposis is a severe, chronic inflammatory condition of the paranasal sinuses and is frequently associated with asthma and aspirin sensitivity. Mesenchymal stem cells exhibit a potent immunosuppressive effect in several inflammatory conditions, and their role in nasal polyposis remains little explored. Hence, we investigated whether bone marrow-derived mesenchymal stem cells could modulate cell phenotype in the nasal polyp milieu. After coculture with mesenchymal stem cells, the frequency of these inflammatory cells was found to decrease. Furthermore, mesenchymal stem cells promoted strong inhibition of CD4+ and CD8+ T cell proliferation, increased the frequency of CD4+CD25+Foxp3 T cells, and changed the global cytokine profile from an inflammatory to an anti-inflammatory response. We believe that mesenchymal stem cells may be a very useful adjunct for investigation of the inflammatory process in nasal polyposis, contributing to better understanding of the inflammatory course of this condition.
Upper Airway Research Laboratory (URL), Department of Otorhinolaryngology, Ghent University Hospital, Ghent University, 9000 Ghent, Belgium ; Department of Otolaryngology-Head and Neck Surgery, Federal University of São Paulo, Rua dos Otonis, 700 Piso Superior, Vila Clementino, 04025-002 São Paulo, SP, Brazil.
Rogério
R
Pezato
Nephrology Division, Department of Medicine, Federal University of São Paulo, 04025-002 São Paulo, SP, Brazil.
Danilo Cândido
DC
de Almeida
Department of Otorhinolaryngology and Ophthalmology, University of São Paulo, 05508-070 São Paulo, SP, Brazil.
Thiago Freire
TF
Bezerra
Department of Immunology, Institute of Biomedical Sciences IV, University of São Paulo, 05508-070 São Paulo, SP, Brazil.
Fernando de Sá
Fde S
Silva
Upper Airway Research Laboratory (URL), Department of Otorhinolaryngology, Ghent University Hospital, Ghent University, 9000 Ghent, Belgium.
Claudina
C
Perez-Novo
Department of Otolaryngology-Head and Neck Surgery, Federal University of São Paulo, Rua dos Otonis, 700 Piso Superior, Vila Clementino, 04025-002 São Paulo, SP, Brazil.
Luís Carlos
LC
Gregório
Department of Otorhinolaryngology and Ophthalmology, University of São Paulo, 05508-070 São Paulo, SP, Brazil.
Richard Louis
RL
Voegels
Department of Immunology, Institute of Biomedical Sciences IV, University of São Paulo, 05508-070 São Paulo, SP, Brazil.
Niels Olsen
NO
Câmara
Upper Airway Research Laboratory (URL), Department of Otorhinolaryngology, Ghent University Hospital, Ghent University, 9000 Ghent, Belgium.
Claus
C
Bachert
D001854:Bone Marrow Cells / Q000166:cytology*; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology; D002478:Cells, Cultured; D018920:Coculture Techniques; D016207:Cytokines / Q000378:metabolism; D051858:Forkhead Transcription Factors / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D007249:Inflammation / Q000276:immunology*; D008213:Lymphocyte Activation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D009298:Nasal Polyps / Q000276:immunology* / Q000473:pathology*; D010641:Phenotype
D016207:Cytokines; C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors