X-ray and neutron investigation of self-assembled lipid layers on a titanium surface.
Titanium is the most widely preferred metal material for bone reconstruction in orthopedics and dentistry. To improve its biological performance, various coatings can be applied. In this investigation, a biomimetic coating on a model implant surface was studied in X-ray and neutron reflectivity experiments to probe the quality of this coating, which is only few nanometers thick. Titanium was deposited on polished silicon surfaces using a magnetron sputtering technique. To improve the lipid coating's stability, a stronger van der Waals interaction was first created between the implant surface and the biomimetic coating by adding a phosphonic acid (n-octadecylphosphonic acid - OPA) monolayer onto the surfaces. Then, three monolayers of POPE (phospholipid 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-ethanolamine) were transferred using the Langmuir-Blodgett (LB) and Langmuir-Schaefer (LS) techniques. The analysis of X-ray and neutron specular reflectivity data shows that OPA molecules cover the model implant surface completely and that approximately 50% coverage of POPE can be achieved by LB and LS transfer.
Institute of Materials Research, Helmholtz-Zentrum Geesthacht Zentrum für Material und Küstenforschung (HZG), Geesthacht, Germany, Maksym.Golub@hzg.de.
Maksym
M
Golub
Dieter
D
Lott
Erik B
EB
Watkins
Vasyl
V
Garamus
Berengere
B
Luthringer
Michael
M
Stoermer
Andreas
A
Schreyer
Regine
R
Willumeit
D008055:Lipids / Q000737:chemistry*; D010714:Phosphatidylethanolamines / Q000737:chemistry; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
D008055:Lipids; D010714:Phosphatidylethanolamines; C057561:1-palmitoyl-2-oleoylphosphatidylethanolamine; D014025:Titanium