Influence of pastes containing casein phosphopeptide-amorphous calcium phosphate on surface of demineralized enamel.
OBJECTIVE
The purpose of the study was to evaluate the surface characteristics of demineralized enamel after treatment with pastes containing casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) or casein phosphopeptide-amorphous calcium fluoride phosphate (CPP-ACFP) and to compare their efficacy with that of 0.05% NaF.


METHODS
Following formation of the artificial carious lesion, enamel slabs were divided into 4 groups (CPP-ACP, CPP-ACFP, 0.05% NaF and control) and submitted to a chemical caries model. Remineralization potential was examined using scanning electron microscope, energy-dispersive spectroscopy (EDS) and microhardness test. Scanning electron microphotographs were analyzed for area, minimal, maximal and mean diameter, perimeter, roundness and number of enamel defects and percentage of tooth surface affected by defects.


RESULTS
Treatment with 0.05% NaF partly reduced the appearance of enamel defects when compared with irregular demineralized enamel. Treatment with CPP-ACP or CPP-ACFP resulted in occlusion of defects which produced more flattened enamel surface. Image analysis revealed reduction of the dimensions of the defects in the 3 experimental groups. Treatment with CPP-ACFP decreased the number of enamel defects when compared with demineralized enamel. The EDS analysis did not show differences in Ca/O, P/O and Ca/P ratios between the groups (P>0.05). Microhardness test revealed significant effects of CPP-ACP and CPP-ACFP (P<0.05).


CONCLUSIONS
Pastes containing CPP-ACP or CPP-ACFP showed potential to remineralize enamel surface lesions.
1 Department of Pediatric and Preventive Dentistry, Faculty of Dentistry, University of Belgrade, Belgrade - Serbia.
Tamara O
TO
Peric
Dejan L J
DL
Markovic
Vesna J
VJ
Radojevic
Radmila M Jancic
RM
Heinemann
Bojan B
BB
Petrovic
Jelena S
JS
Lamovec
D002364:Caseins / Q000008:administration & dosage* / Q000737:chemistry*; D003731:Dental Caries / Q000188:drug therapy* / Q000473:pathology*; D003804:Dentin / Q000737:chemistry* / Q000187:drug effects / Q000648:ultrastructure; D006801:Humans; D066298:In Vitro Techniques; D008422:Materials Testing; D009824:Ointments; D013499:Surface Properties; D014089:Tooth Remineralization / Q000379:methods*; D016896:Treatment Outcome
D002364:Caseins; C093073:Demineralized Dentin Matrix; D009824:Ointments; C447032:casein phosphopeptide-amorphous calcium phosphate nanocomplex