Differential expression of ARID3B in normal adult tissue and carcinomas.
ARID3B is a DNA binding protein that is overexpressed in neuroblastoma and ovarian cancer. To understand the extent that ARID3B participates in tumor development, we assessed protein expression of ARID3B in normal adult and malignant tissues. We found that ARID3B is highly expressed in differentiated layers of squamous epithelium. We also examined expression of an alternative splice form of ARID3B and found that it has similar but not identical expression patterns to the full length ARID3B isoform. ARID3B has two closely related paralogues, ARID3A and ARID3C. Each of these 3 family members exhibits different patterns of expression. Of the ARID3 family members, ARID3B is the most widely expressed and is particularly expressed in epithelium. In addition to examining normal tissue, we investigated ARID3B expression in a variety of tumor types. Most notably we found that ARID3B expression is decreased in esophagus and stomach tumors compared to normal corresponding tissues. Our results indicate that the different patterns of ARID3B in normal tissues translate into different roles for ARID3B in carcinomas.
Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, South Bend, IN, USA. Electronic address: serenejudith@gmail.com.
Serene J
SJ
Samyesudhas
Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, South Bend, IN, USA. Electronic address: roy.lynnm@gmail.com.
Lynn
L
Roy
Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, South Bend, IN, USA; Department of Chemistry and Biochemistry, Notre Dame University, Notre Dame, IN, USA; Eck Institute for Global Health, Notre Dame University, Notre Dame, IN, USA; Indiana University Melvin and Bren Simon Cancer Center, Indianapolis, IN, USA. Electronic address: kcowdend@iupui.edu.
Karen D
KD
Cowden Dahl
D000328:Adult; D017398:Alternative Splicing / Q000235:genetics; D000818:Animals; D002277:Carcinoma / Q000235:genetics* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D004848:Epithelium / Q000378:metabolism; D005260:Female; D005333:Fetus / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D051379:Mice; D014018:Tissue Distribution
C120893:ARID3B protein, human; C510769:Arid3b protein, mouse; D004268:DNA-Binding Proteins
ARID3B; Differentiation; Immunohistochemistry; Stratified epithelium; Transcription factor