Detection of breast cancer from blood through analysis of lymphocyte fluorescent intensity using MUC1 antigen.
BACKGROUND
Sensitive and reliable early diagnostic markers for breast cancer (BC) are still unavailable today. In this work, we proposed a new complementary method for detection of BC. This method is based on an observation that lymphocytes re-exposed in vitro to antigenic stimulation express cytoplasmic changes.


METHODS
In the new protocol, we recorded changes in the fluorescence intensity of light emitted from lymphocytes obtained from females with and without BC after stimulation with MUC1 antigen utilized flow cytometry.


RESULTS
Out of 55 BC patients tested, 46 were correctly diagnosed. Of 73 controls, 55 were correctly identified as healthy subjects. The sensitivity of the test was 84 %; the specificity was 75 %.


CONCLUSIONS
These results suggest a potentially valuable method for detection of BC. The clinical importance of this procedure relies on the ability to screen populations for BC with widely available flow cytometry by a relatively fast, accurate, and economical procedure. Another potential benefit would be identification of candidates for vaccination as a primary or secondary preventive measure.
Research Department, Ziv Medical Center, Safed, Israel. ayelet.o@ziv.health.gov.il.
Ayelet
A
Armon-Omer
Surgery Department, Ziv Medical Center, Safed, Israel.|Faculty of Medicine, Bar-Ilan University, 14302, Safed, Israel.
Amram
A
Hadary
Research Department, Ziv Medical Center, Safed, Israel.
Georgette
G
Hilu
Research Department, Ziv Medical Center, Safed, Israel.
Bat-El
BE
Tayar
Research Department, Ziv Medical Center, Safed, Israel.
Tali
T
Keren
Research Department, Ziv Medical Center, Safed, Israel.
Adi
A
Sharabi-Nov
Surgery Department, Western Galilee Hospital, Nahariya, Israel.|Faculty of Medicine, Bar-Ilan University, 14302, Safed, Israel.
Amitai
A
Bickel
Research Department, Ziv Medical Center, Safed, Israel.
Ofer
O
Klein
D000328:Adult; D014408:Biomarkers, Tumor / Q000097:blood; D001943:Breast Neoplasms / Q000097:blood* / Q000175:diagnosis* / Q000276:immunology; D016022:Case-Control Studies; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D008214:Lymphocytes / Q000737:chemistry* / Q000187:drug effects / Q000276:immunology; D008875:Middle Aged; D018396:Mucin-1 / Q000276:immunology / Q000494:pharmacology*; D012680:Sensitivity and Specificity
D014408:Biomarkers, Tumor; D018396:Mucin-1
Breast; Cancer; Flow cytometry; Lymphocyte; MUC1