Arginase and C-reactive protein as potential serum-based biomarker of head and neck squamous cell carcinoma patients of north east India.
Head and neck squamous cell carcinoma (HNSCC) diagnosis involves histopathological investigations with intervention procedures. Widely accepted biochemical panels for HNSCC detection is not available yet. We tried to find out the effectiveness of the serum-based biochemical markers for their prognostic significance in HNSCC. We collected fresh blood samples of 80 HNSCC patients and 100 healthy subjects. Samples were analyzed for absolute levels of arginase (ARG), ornithine aminotransferase (OAT), catalase (CAT), lactate dehydrogenase (LDH), amylase (AMY) activity, and C-reactive protein (CRP). Receiver-operator characteristic curves and Fagan's nomograms were generated. Out of six parameters studied, four (OAT, CAT, LDH, and AMY) did not show biologically significant differences to be used as biomarkers. Significant elevation in serum ARG and CRP levels were observed in HNSCC. The individual estimation of ARG and CRP showed lower specificity, but their combination improved the specificity level to 95%. Our results suggest that serum ARG and CRP together can efficiently diagnose HNSCC.
Molecular Medicine Lab., Department of Biotechnology, Assam University, Silchar, PIN-788011, Assam, India.
Biswadeep
B
Choudhury
Shilpee
S
Srivastava
Haider H
HH
Choudhury
Alokananda
A
Purkayastha
Sumita
S
DuttaGupta
Sankar Kumar
SK
Ghosh
D000328:Adult; D000681:Amylases / Q000097:blood; D001119:Arginase / Q000097:blood; D014408:Biomarkers, Tumor / Q000097:blood*; D002097:C-Reactive Protein / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000097:blood* / Q000175:diagnosis / Q000473:pathology; D005260:Female; D006258:Head and Neck Neoplasms / Q000097:blood* / Q000175:diagnosis / Q000473:pathology; D006801:Humans; D007194:India; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008297:Male; D008875:Middle Aged; D011379:Prognosis
D014408:Biomarkers, Tumor; D002097:C-Reactive Protein; D007770:L-Lactate Dehydrogenase; D000681:Amylases; D001119:Arginase