Robust overlay schemes for the fusion of fluorescence and color channels in biological imaging.
Molecular fluorescence imaging is a commonly used method in various biomedical fields and is undergoing rapid translation toward clinical applications. Color images are commonly superimposed with fluorescence measurements to provide orientation, anatomical information, and molecular tissue properties in a single image. New adaptive methods that produce a more robust composite image than conventional lime green alpha blending are presented and demonstrated herein. Moreover, visualization through temporal changes is showcased as an alternative for real-time imaging systems.
Technische Universität München, Chair for Biological Imaging, Trogerstr. 9, D-81675 Munich, Germany.
Jürgen
J
Glatz
Institute for Biological and Medical Imaging, Helmholtz Zentrum München, Ingolstädter Landstr. 1, D-85764 Neuherberg, Germany.
Panagiotis
P
Symvoulidis
Technische Universität München, Chair for Biological Imaging, Trogerstr. 9, D-81675 Munich, Germany.
P Beatriz
PB
Garcia-Allende
Technische Universität München, Chair for Biological Imaging, Trogerstr. 9, D-81675 Munich, GermanybInstitute for Biological and Medical Imaging, Helmholtz Zentrum München, Ingolstädter Landstr. 1, D-85764 Neuherberg, Germany.
Vasilis
V
Ntziachristos
D000818:Animals; D005456:Fluorescent Dyes; D049452:Green Fluorescent Proteins; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D051379:Mice; D061848:Optical Imaging / Q000379:methods*; D018515:Plant Leaves; D013050:Spectrometry, Fluorescence / Q000379:methods*; D015027:Zebrafish
D005456:Fluorescent Dyes; D049452:Green Fluorescent Proteins