Analysis of affinity of dengue virus protein interaction using Biacore.
Surface plasmon resonance (SPR) biosensors have become the mainstream method for biomolecular interaction analysis. It offers many advantages over conventional methods by its label-free, real-time monitoring, low sample consumption, high throughput, and remarkable sensitivity. We have examined dengue virus protein interactions in the context of antibody affinity measurement, protein-protein interaction, and in the screening of small molecule inhibitors as well as the characterization of the interactions between the small molecule binders and the relevant dengue protein. Here we describe the basic methods involved in performing SPR assays as well as in data processing and evaluation using some examples of dengue proteins.
Division of Chemical Biology and Biotechnology, School of Biological Sciences, Nanyang Technological University, Singapore, Singapore.
Yin Hoe
YH
Yau
Susana Geifman
SG
Shochat
D015374:Biosensing Techniques / Q000379:methods*; D002021:Buffers; D003716:Dengue Virus / Q000378:metabolism*; D007700:Kinetics; D008024:Ligands; D008958:Models, Molecular; D011485:Protein Binding; D014764:Viral Proteins / Q000378:metabolism*
D002021:Buffers; D008024:Ligands; D014764:Viral Proteins