Biochemical changes and oxidative stress induced by zearalenone in the liver of pregnant rats.
The aim of the present research was to examine the toxic influence of different doses of zearalenone (ZEN) on the liver, especially oxidative stress induced by ZEN on the liver. A total of 48 pregnant Sprague-Dawley rats were randomly assigned into 4 treatments groups with 12 animals in each. The rats were fed with a normal diet treated with 0 mg/kg (control), 50 mg/kg (treatment 1), 100 mg/kg (treatment 2), or 150 mg/kg (treatment 3) ZEN in feed on gestation days (GDs) 0-7 and then all the rats were fed with a normal diet on GDs 8-20. The experimental period lasted 21 days. The results showed that exposure to ZEN induced increase in aspartate amino transferase, alanine aminotransferase, and alkaline phosphatase activities and decrease in total protein and albumin content in a dose-dependent manner and also induce decrease in superoxide dismutase and glutathione peroxidase activities and increase in malondialdehyde content in a dose-dependent manner in the serum and the liver. The increased transcription of cytochrome P450 2E1 (CYP2E1) was detected in the liver after exposure to ZEN. These results suggested that ZEN not only caused damage in the liver of pregnant rats in a dose-dependent manner but also induced the messenger RNA expression of CYP2E1 in the liver.
Institute of Animal Nutrition, Northeast Agricultural University, Harbin, People's Republic of China.
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Zhou
Institute of Animal Nutrition, Northeast Agricultural University, Harbin, People's Republic of China.
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Zhang
Institute of Animal Nutrition, Northeast Agricultural University, Harbin, People's Republic of China.
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Yin
Institute of Animal Nutrition, Northeast Agricultural University, Harbin, People's Republic of China.
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Jia
Institute of Animal Nutrition, Northeast Agricultural University, Harbin, People's Republic of China asshan@neau.edu.cn.
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Shan
D000410:Alanine Transaminase / Q000097:blood; D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001663:Bilirubin / Q000097:blood; D019392:Cytochrome P-450 CYP2E1 / Q000235:genetics; D005260:Female; D014453:Glucuronosyltransferase; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D011247:Pregnancy; D012333:RNA, Messenger / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D012709:Serum Albumin / Q000032:analysis; D015025:Zearalenone / Q000494:pharmacology*; D000099148:UGT1A1 Enzyme
D000410:Alanine Transaminase; D000469:Alkaline Phosphatase; D001219:Aspartate Aminotransferases; D001663:Bilirubin; D019392:Cytochrome P-450 CYP2E1; D014453:Glucuronosyltransferase; D012333:RNA, Messenger; D012709:Serum Albumin; D015025:Zearalenone; C499946:Ugt1a1 protein, rat; D000099148:UGT1A1 Enzyme
Zearalenone; biochemical changes; liver; oxidative stress; pregnant rats