Electrical and neurotrophin enhancement of neurite outgrowth within a 3D collagen scaffold.
Electrical and chemical stimulation have been studied as potent mechanisms of enhancing nerve regeneration and wound healing. However, it remains unclear how electrical stimuli affect nerve growth, particularly in the presence of neurotrophic factors. The objective of this study was to explore (1) the effect of brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) supplementation to support neurite outgrowth in a 3D scaffold, and (2) the effect of brief, low voltage, electrical stimulation (ES) on neurite outgrowth prior to neurotrophin supplementation. Dissociated E11 chick dorsal root ganglia (DRG) were seeded within a 1.5 mg/mL type-I collagen scaffold. For neurotrophin treatments, scaffolds were incubated for 24 h in culture media containing NGF (10 ng/mL) or BDNF (200 ng/mL), or both. For ES groups, scaffolds containing neurons were stimulated for 10 min at 8-10 V/m DC, then incubated for 24 h with neurotrophin. Fixed and labeled neurons were imaged to measure neurite growth and directionality. BDNF supplementation was not as effective as NGF at supporting DRG neurite outgrowth. ES prior to NGF supplementation improved DRG neurite outgrowth compared to NGF alone. This combination of brief ES with NGF treatment was the most effective treatment compared to NGF or BDNF alone. Brief ES had no impact on neurite directionality in the 3D scaffolds. These results demonstrate that ES improves neurite outgrowth in the presence of neurotrophins, and could provide a potential therapeutic approach to improve nerve regeneration when coupled with neurotrophin treatment.
Department of Pharmacological and Physiological Science, Saint Louis University, 1402 S. Grand Blvd, St. Louis, MO, 63104, USA.
Robert D
RD
Adams
Sara R
SR
Rendell
Lauren R
LR
Counts
Jason B
JB
Papke
Rebecca K
RK
Willits
Amy B
AB
Harkins
D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism; D002478:Cells, Cultured; D002642:Chick Embryo; D003094:Collagen / Q000737:chemistry*; D004558:Electric Stimulation; D005727:Ganglia, Spinal / Q000166:cytology / Q000378:metabolism*; D020932:Nerve Growth Factor / Q000378:metabolism; D009414:Nerve Growth Factors / Q000378:metabolism; D016501:Neurites / Q000378:metabolism*; D054457:Tissue Scaffolds / Q000737:chemistry*
D019208:Brain-Derived Neurotrophic Factor; D009414:Nerve Growth Factors; D003094:Collagen; D020932:Nerve Growth Factor