Bacterial cellulose and hyaluronic acid hybrid membranes: Production and characterization.
In this study, the effect of the addition of hyaluronic acid (HA) on bacterial cellulose (BC) production, under static conditions was evaluated in terms of the properties of the resulting BC hybrid membranes. HA was added to the fermentation process in three distinct time points: first day (BC-T0), third day (BC-T3) and sixth day (BC-T6). Analyses of FT-IR and CP/MAS (13)C NMR confirmed the presence of HA in bacterial cellulose membranes. The crystal structure, crystallinity index (Ic) surface roughness, thermal stability and hybrophobic/hydrophilic character changed. Membranes with higher roughness were produced with HA added on the first and third day of fermentation process. The surface energy of BC/HA membranes was calculated and more hydrophilic membranes were produced by the addition of HA on the third and sixth day, also resulting in more thermally stable materials. The results demonstrate that bacterial cellulose/hyaluronic acid hybrid membranes can be produced in situ and suggest that HA interacts with the sub-elementary bacterial cellulose fibrils, changing the properties of the membranes. The study and understanding of the factors that affect those properties are of utmost importance for the safe and efficient use of BC as biomaterials in numerous applications, specifically in the biological field.
Department of Biochemistry and Biotechnology, CCE, State University of Londrina, PO Box 6001, 86051-990 Londrina, PR, Brazil.
Tatyane Duran
TD
Lopes
Departamento de Química, Universidade Federal do Paraná (UFPR), CxP 19081, CEP 81531-980 Curitiba, PR, Brazil. Electronic address: izabel.riegel@ufpr.br.
Izabel Cristina
IC
Riegel-Vidotti
Departamento de Química, Universidade Federal do Paraná (UFPR), CxP 19081, CEP 81531-980 Curitiba, PR, Brazil.
Aline
A
Grein
Department of Biochemistry and Biotechnology, CCE, State University of Londrina, PO Box 6001, 86051-990 Londrina, PR, Brazil. Electronic address: cesar.tischer@uel.br.
Cesar Augusto
CA
Tischer
Department of Biochemistry and Biotechnology, CCE, State University of Londrina, PO Box 6001, 86051-990 Londrina, PR, Brazil. Electronic address: paula.tischer@pq.cnpq.br.
Paula Cristina de Sousa
PC
Faria-Tischer
D001419:Bacteria / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry; D002482:Cellulose / Q000138:chemical synthesis / Q000737:chemistry*; D018360:Crystallography, X-Ray; D005285:Fermentation; D006820:Hyaluronic Acid / Q000138:chemical synthesis / Q000737:chemistry*; D008567:Membranes, Artificial; D017550:Spectroscopy, Fourier Transform Infrared; D054457:Tissue Scaffolds / Q000737:chemistry
D001672:Biocompatible Materials; D008567:Membranes, Artificial; D002482:Cellulose; D006820:Hyaluronic Acid
Bacterial cellulose; Hyaluronic acid; Hybrid membranes; Properties; Scaffolds