Highly flexible and rapidly degradable papaverine-loaded electrospun fibrous membranes for preventing vasospasm and repairing vascular tissue.
Vasospasm is a common post-operative complication after vascular anastomosis. Currently, the main treatment is a local injection of antispasmodic drugs. However, this method has a high rate of relapse and is subject to a large degree of individual variation, and repeated injections cause additional pain for patients. In this study, we developed highly flexible and rapidly degradable papaverine-loaded electrospun fibrous membranes to be wrapped around vascular suturing to prevent vasospasm. Poly-l-lactic acid/polyethylene glycol (PLLA/PEG) electrospun fibers containing papaverine maintained a high degree of flexibility and could withstand any folding, and are therefore suitable for wrapping vascular suturing. A rapid release of papaverine, between 2 and 7 days, was achieved by adjusting the proportions of PEG and PLLA. PLLA electrospun fibers containing 40% PEG (PLLA-40%) could control drug release and polymer degradation most effectively during the first 2 weeks post-operation. Testing using an in vivo rabbit model showed that PLLA-40% fibrous membranes produced significant antispasmodic effect without observable inflammation or hyperplasia, and the fibrous membranes were ideally biodegradable, with no impact on regional blood flow, pressure, vessel diameter or surrounding tissue hyperplasia. Therefore, papaverine-loaded electrospun fibrous membranes show the potential to greatly reduce post-operative vasospasm and maintain regular vascular morphology during antispasmodic therapy.
Orthopedic Institute, Soochow University, 708 Renmin Rd, Suzhou, Jiangsu 215006, People's Republic of China; School of Biomedical Engineering and Med-X Research Institute, Shanghai Jiao Tong University, 1954 Huashan Rd, Shanghai 200030, People's Republic of China.
Wankun
W
Zhu
Department of Orthopaedics, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University School of Medicine, 600 Yishan Rd, Shanghai 200233, People's Republic of China.
Shenghe
S
Liu
Orthopedic Institute, Soochow University, 708 Renmin Rd, Suzhou, Jiangsu 215006, People's Republic of China; School of Biomedical Engineering and Med-X Research Institute, Shanghai Jiao Tong University, 1954 Huashan Rd, Shanghai 200030, People's Republic of China.
Jingwen
J
Zhao
Department of Orthopaedics, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University School of Medicine, 600 Yishan Rd, Shanghai 200233, People's Republic of China.
Shen
S
Liu
Department of Orthopaedics, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University School of Medicine, 600 Yishan Rd, Shanghai 200233, People's Republic of China.
Shichao
S
Jiang
Orthopedic Institute, Soochow University, 708 Renmin Rd, Suzhou, Jiangsu 215006, People's Republic of China; Department of Orthopedics, The First Affiliated Hospital of Soochow University, 188 Shizi St, Suzhou, Jiangsu 215006, People's Republic of China.
Bin
B
Li
Orthopedic Institute, Soochow University, 708 Renmin Rd, Suzhou, Jiangsu 215006, People's Republic of China; Department of Orthopedics, The First Affiliated Hospital of Soochow University, 188 Shizi St, Suzhou, Jiangsu 215006, People's Republic of China.
Huilin
H
Yang
Department of Orthopaedics, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University School of Medicine, 600 Yishan Rd, Shanghai 200233, People's Republic of China. Electronic address: fancunyi888@hotmail.com.
Cunyi
C
Fan
Orthopedic Institute, Soochow University, 708 Renmin Rd, Suzhou, Jiangsu 215006, People's Republic of China; Department of Orthopedics, The First Affiliated Hospital of Soochow University, 188 Shizi St, Suzhou, Jiangsu 215006, People's Republic of China. Electronic address: wgcui80@hotmail.com.
Wenguo
W
Cui
D000818:Animals; D003329:Coronary Vasospasm / Q000517:prevention & control*; D008567:Membranes, Artificial; D008855:Microscopy, Electron, Scanning; D010208:Papaverine / Q000008:administration & dosage* / Q000097:blood; D011817:Rabbits; D018615:Ultrasonography, Doppler, Color
D008567:Membranes, Artificial; D010208:Papaverine
Electrospun fibers; Papaverine; Rapid degradation; Vascular; Vasospasm