Extracellular matrix production in vitro in cartilage tissue engineering.
Cartilage tissue engineering is arising as a technique for the repair of cartilage lesions in clinical applications. However, fibrocartilage formation weakened the mechanical functions of the articular, which compromises the clinical outcomes. Due to the low proliferation ability, dedifferentiation property and low production of cartilage-specific extracellular matrix (ECM) of the chondrocytes, the cartilage synthesis in vitro has been one of the major limitations for obtaining high-quality engineered cartilage constructs. This review discusses cells, biomaterial scaffolds and stimulating factors that can facilitate the cartilage-specific ECM production and accumulation in the in vitro culture system. Special emphasis has been put on the factors that affect the production of ECM macromolecules such as collagen type II and proteoglycans in the review, aiming at providing new strategies to improve the quality of tissue-engineered cartilage.
Jie-Lin
JL
Chen
Li
L
Duan
Weimin
W
Zhu
Jianyi
J
Xiong
Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Second People's Hospital (The First Affiliated Hospital of Shenzhen University), Shenzhen 518035, Guangdong Province, China. dapingwang1963@qq.com.
Daping
D
Wang
D000818:Animals; D002358:Cartilage, Articular / Q000378:metabolism*; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis*; D015316:Genetic Therapy; D006801:Humans; D066298:In Vitro Techniques; D023822:Tissue Engineering; D054457:Tissue Scaffolds
D016326:Extracellular Matrix Proteins