TAL effector-mediated genome visualization (TGV).
The three-dimensional remodeling of chromatin within nucleus is being recognized as determinant for genome regulation. Recent technological advances in live imaging of chromosome loci begun to explore the biological roles of the movement of the chromatin within the nucleus. To facilitate better understanding of the functional relevance and mechanisms regulating genome architecture, we applied transcription activator-like effector (TALE) technology to visualize endogenous repetitive genomic sequences in mouse cells. The application, called TAL effector-mediated genome visualization (TGV), allows us to label specific repetitive sequences and trace nuclear remodeling in living cells. Using this system, parental origin of chromosomes was specifically traced by distinction of single-nucleotide polymorphisms (SNPs). This review will present our approaches to monitor nuclear dynamics of target sequences and highlights key properties and potential uses of TGV.
Okazaki Institute for Integrative Bioscience, Okazaki, Japan. Electronic address: miyanari@nibb.ac.jp.
Yusuke
Y
Miyanari
D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis* / Q000235:genetics*; D018390:Gene Targeting / Q000379:methods*; D016678:Genome; D057809:HEK293 Cells; D006801:Humans; D049409:Luciferases, Firefly; D049410:Luciferases, Renilla; D051379:Mice; D008969:Molecular Sequence Data
D001426:Bacterial Proteins; D049410:Luciferases, Renilla; D049409:Luciferases, Firefly
Live imaging; Nuclear architecture; TAL effector