Effects of dietary cholesterol and its oxidation products on pathological lesions and cholesterol and lipid oxidation in the rabbit liver.
This study was conducted to determine the effects of dietary cholesterol (CHO) and cholesterol oxidation products (COPs) on the induction of pathological lesions in rabbit liver tissues. Liver lesions were induced only when the levels of CHO and COPs in the diet were very high. The amount of CHO measured in the liver increased when dietary CHO was increased; by comparison, dietary COPs affected liver CHO amounts to a lesser extent. The TBARS (thiobarbituric acid reactive substances) value measured for the liver samples also increased when dietary CHO and COP levels were elevated, and the TBARS value was more strongly affected by the amount of COPs in the diet than by the amount of CHO. At 6 and 12 weeks, COP levels were the highest in the group that received 1.2 g CHO + 0.8 g COPs, followed by the 0.5 g CHO + 0.5 g COPs and 1.6 g CHO + 0.4 g COPs groups; the control (0 g) group showed the lowest COP levels among all groups. In this study, we found that not only dietary CHO but also COPs were involved in hypercholesterolemia induced liver lesions when the amount of CHO and COPs was high.
Department of Animal Science and Technology, Chung-Ang University, 4726 Seodong-daero, Daedeok-myeon, Anseong-si, Gyeonggi-do 456-756, Republic of Korea.
Sun Jin
SJ
Hur
Department of Animal Science and Technology, Sunchon National University, Suncheon 540-742, Republic of Korea.
Ki Chang
KC
Nam
Food Science and Technology Program, Department of Agriculture, Food, and Resource Sciences, University of Maryland Eastern Shore, Princess Anne, MD 21853, USA.
Byungrok
0000-0003-4108-6579
B
Min
Department of Animal Science, Washington State University, Pullman, WA 99164-6310, USA.
Min
0000-0002-7232-072X
M
Du
Department of Food and Nutrition, Sunchon National University, Suncheon 540-742, Republic of Korea.
Kwon Il
KI
Seo
Department of Animal Science and Technology, Sunchon National University, Suncheon 540-742, Republic of Korea ; Department of Animal Science, College of Agriculture, Iowa State University, Ames, IA 50011, USA.
Dong Uk
DU
Ahn
D000818:Animals; D002791:Cholesterol, Dietary / Q000494:pharmacology*; D005227:Fatty Acids / Q000378:metabolism; D050356:Lipid Metabolism / Q000187:drug effects*; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D008297:Male; D010084:Oxidation-Reduction / Q000187:drug effects; D011817:Rabbits; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D002791:Cholesterol, Dietary; D005227:Fatty Acids; D017392:Thiobarbituric Acid Reactive Substances