Bioengineered collagens: emerging directions for biomedical materials.
Mammalian collagen has been widely used as a biomedical material. Nevertheless, there are still concerns about the variability between preparations, particularly with the possibility that the products may transmit animal-based diseases. Many groups have examined the possible application of bioengineered mammalian collagens. However, translating laboratory studies into large-scale manufacturing has often proved difficult, although certain yeast and plant systems seem effective. Production of full-length mammalian collagens, with the required secondary modification to give proline hydroxylation, has proved difficult in E. coli. However, recently, a new group of collagens, which have the characteristic triple helical structure of collagen, has been identified in bacteria. These proteins are stable without the need for hydroxyproline and are able to be produced and purified from E. coli in high yield. Initial studies indicate that they would be suitable for biomedical applications.
CSIRO Materials Science and Engineering; Clayton, Australia.
John A M
JA
Ramshaw
CSIRO Materials Science and Engineering; Clayton, Australia.
Jerome A
JA
Werkmeister
CSIRO Materials Science and Engineering; Clayton, Australia.
Geoff J
GJ
Dumsday
D001426:Bacterial Proteins / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry*; D001697:Biomedical and Dental Materials / Q000737:chemistry*; D003094:Collagen / Q000096:biosynthesis*; D006909:Hydroxyproline / Q000737:chemistry; D015202:Protein Engineering; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000737:chemistry
D001426:Bacterial Proteins; D001672:Biocompatible Materials; D001697:Biomedical and Dental Materials; D011994:Recombinant Proteins; D003094:Collagen; D006909:Hydroxyproline
biomedical material; collagen; prokaryote; recombinant expression; thermal stability; tissue engineering; triple helix