Nitrite treatment rescues cardiac dysfunction in aged mice treated with conjugated linoleic acid.
Conjugated linoleic acid (cLA) is a commercially available weight-loss supplement that is not currently regulated by the U.S. FDA. Numerous studies suggest that cLA mediates protection against diseases including cancer, diabetes, atherosclerosis, immune function, and obesity. Based upon these reports, it was hypothesized that supplementation with cLA would improve heart function in aged wild-type (WT) mice. At 10 months of age, mice were treated with cLA, nitrite, or the combination of the two. Echocardiograms revealed that cardiac function was decreased in aged compared to young WT mice, as determined by percentage of fractional shortening. Also, contrary to the hypothesis, mice that received cLA (6-week treatment) had significantly worse cardiac function compared to controls. This effect was attenuated when mice were cotreated with cLA and nitrite. Taken together, these results suggest that cLA-mediated cardiac injury can be circumvented by nitrite supplementation in a murine model of aging.
Department of Biochemistry and Molecular Biology, School of Medicine, University of Louisville, Louisville, KY 40202, USA.
Kellianne M
KM
Piell
Department of Biochemistry and Molecular Biology, School of Medicine, University of Louisville, Louisville, KY 40202, USA; Department of Physiology and Biophysics, School of Medicine, University of Louisville, Louisville, KY 40202, USA.
Natia
N
Qipshidze Kelm
Department of Biochemistry and Molecular Biology, School of Medicine, University of Louisville, Louisville, KY 40202, USA.
Megan P
MP
Caroway
Department of Biochemistry and Molecular Biology, School of Medicine, University of Louisville, Louisville, KY 40202, USA.
Masarath
M
Aman
Department of Biochemistry and Molecular Biology, School of Medicine, University of Louisville, Louisville, KY 40202, USA; Department of Physiology and Biophysics, School of Medicine, University of Louisville, Louisville, KY 40202, USA. Electronic address: marcie.cole@louisville.edu.
Marsha P
MP
Cole
D000375:Aging; D000818:Animals; D015153:Blotting, Western; D002417:Cattle; D042783:Endothelial Cells / Q000187:drug effects*; D006801:Humans; D044243:Linoleic Acids, Conjugated / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009566:Nitrates / Q000494:pharmacology*; D016277:Ventricular Function, Left / Q000187:drug effects*
D044243:Linoleic Acids, Conjugated; D009566:Nitrates
Conjugated linoleic acid; Free radicals; Heart function; Nitrite; eNOS