Mechanically modulating the photophysical properties of fluorescent protein biocomposites for ratio- and intensiometric sensors.
Mechanically sensitive biocomposites comprised of fluorescent proteins report stress through distinct pathways. Whereas a composite containing an enhanced yellow fluorescent protein (eYFP) exhibited hypsochromic shifts in its fluorescence emission maxima following compression, a composite containing a modified green fluorescent protein (GFPuv) exhibited fluorescence quenching under the action of mechanical force. These ratio- and intensiometric sensors demonstrate that insights garnered from disparate fields (that is, polymer mechanochemistry and biophysics) can be harnessed to guide the rational design of new classes of biomechanophore-containing materials.
Department of Chemistry, University of Texas at Austin, 1 University Station A1590, Austin, TX 78712 (USA) http://keatinge-clay.cm.utexas.edu http://bielawski.cm.utexas.edu.
Johnathan N
JN
Brantley
Constance B
CB
Bailey
Joe R
JR
Cannon
Katie A
KA
Clark
David A
DA
Vanden Bout
Jennifer S
JS
Brodbelt
Adrian T
AT
Keatinge-Clay
Christopher W
CW
Bielawski
D049452:Green Fluorescent Proteins / Q000737:chemistry*; D008027:Light; D010825:Physics
D049452:Green Fluorescent Proteins
biocomposites; fluorescent protein; mechanochemistry; sensors; stimulus-responsive materials