Improved reagents for newborn screening of mucopolysaccharidosis types I, II, and VI by tandem mass spectrometry.
Tandem mass spectrometry for the multiplex and quantitative analysis of enzyme activities in dried blood spots on newborn screening cards has emerged as a powerful technique for early assessment of lysosomal storage diseases. Here we report the design and process-scale synthesis of substrates for the enzymes α-l-iduronidase, iduronate-2-sulfatase, and N-acetylgalactosamine-4-sulfatase that are used for newborn screening of mucopolysaccharidosis types I, II, and VI. The products contain a bisamide unit that is hypothesized to readily protonate in the gas phase, which improves detection sensitivity by tandem mass spectrometry. The products contain a benzoyl group, which provides a useful site for inexpensive deuteration, thus facilitating the preparation of internal standards for the accurate quantification of enzymatic products. Finally, the reagents are designed with ease of synthesis in mind, thus permitting scale-up preparation to support worldwide newborn screening of lysosomal storage diseases. The new reagents provide the most sensitive assay for the three lysosomal enzymes reported to date as shown by their performance in reactions using dried blood spots as the enzyme source. Also, the ratio of assay signal to that measured in the absence of blood (background) is superior to all previously reported mucopolysaccharidosis types I, II, and VI assays.
Departments of Chemistry, ‡Pediatrics, and §Biochemistry, University of Washington , Seattle, Washington 98195-1700, United States.
Naveen Kumar
NK
Chennamaneni
Arun Babu
AB
Kumar
Mariana
M
Barcenas
Zdeněk
Z
Spáčil
C Ronald
CR
Scott
František
F
Tureček
Michael H
MH
Gelb
D006801:Humans; D007202:Indicators and Reagents / Q000737:chemistry*; D007231:Infant, Newborn; D009083:Mucopolysaccharidoses / Q000145:classification / Q000175:diagnosis*; D015997:Neonatal Screening; D053719:Tandem Mass Spectrometry / Q000379:methods*
D007202:Indicators and Reagents