Delta-ALA-D inhibitory potential and protective action of Syzygium jambos and Solanum guaraniticum leaf extracts on oxidatively stressed erythrocytes.
OBJECTIVE
This study aimed to investigate the effect of the leaf extracts of Syzygium jambos and Solanum guaraniticum on the δ-aminolevulinate dehydratase (δ-ALA-D) activity, their antioxidant activity and potential protective action on oxidatively stressed erythrocytes, in order to demonstrate the safety or toxicity of the plant.


METHODS
In erythrocyte samples, the effect of both extracts on δ-ALA-D activity, H2O2-induced oxidative stress, and 2,2'azobis (2-amidinopropane) (AAPH)-induced hemolysis was evaluated, as well as some antioxidant mechanisms.


RESULTS
Both extracts inhibited δ-ALA-D activity (S. guaraniticum > S. jambos), and an involvement of the zinc ion of the δ-ALA-D structure on the inhibition of enzyme activity was verified. S. jambos leaf extract showed marked efficiency in countering H2O2-induced lipid peroxidation and in maintaining cellular integrity against AAPH-induced hemolysis. Furthermore, S. jambos exhibited greater H2O2 scavenging activity and stronger reduction power than S. guaraniticum.


CONCLUSIONS
Both extracts bear potent antioxidant property as an important beneficial effect. However, the inhibition of δ-ALA-D activity suggests a possible harmful effect of these vegetal preparations and indicates the need for further investigation regarding their toxicological properties. All together, these data represent a significant contribution to the knowledge of these plants, both to the scientific community and to the folk medicine.
Gabriela
G
Bonfanti
Karine Santos De
KS
Bona
Leidiane de
Ld
Lucca
Leticia
L
Jantsch
Aline Schirmer
AS
Pigatto
Aline A
AA
Boligon
Margareth L
ML
Athayde
Maria Beatriz
MB
Moretto
Thissiane de Lima
Tde L
Gonçalves
D000578:Amidines / Q000494:pharmacology; D000975:Antioxidants / Q000378:metabolism; D004912:Erythrocytes / Q000187:drug effects* / Q000201:enzymology; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects; D016877:Oxidants / Q000494:pharmacology; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D000623:Porphobilinogen Synthase / Q000378:metabolism*; D032322:Solanum / Q000737:chemistry*; D027842:Syzygium / Q000737:chemistry*
D000578:Amidines; D000975:Antioxidants; D016877:Oxidants; D010936:Plant Extracts; C046728:2,2'-azobis(2-amidinopropane); D006861:Hydrogen Peroxide; D000623:Porphobilinogen Synthase
Antioxidant; Delta-aminolevulinate dehydratase; Erythrocytes; Hemolysis; Solanum guaraniticum; Syzygium jambos