Identification and pharmacokinetics of multiple constituents in rat plasma after oral administration of Yinchenzhufu decoction.
BACKGROUND
Yinchenzhufu decoction (YCZFD) is a classical Chinese herbal formula and has been used to treat severe jaundice in chronic liver injuries since the Qing Dynasty (18th century CE). To identify the components absorbed into the blood in YCZFD and explore their pharmacokinetic profile for understanding the effective ingredients of YCZFD.


METHODS
After rats were given YCZFD by intragastric administration, the plasma was processed by precipitation of protein. The compounds in YCZFD extract and the plasma were identified by using high-resolution mass spectrometry with a database-directed strategy. The pharmacokinetics of multiple compounds from YCZFD in rat plasma was studied by using the established UPLC-MS/MS method.


RESULTS
Forty compounds in YCZFD extract and 21 prototype compounds with 11 metabolites in rat plasma were detected after oral administration. The pharmacokinetic parameters of glycyrrhizic acid, glycyrrhetic acid, cinnamic acid, ononin, atractylenolide III, and liquiritin from YCZFD were obtained in rats.


CONCLUSIONS
The identified constituents and the pharmacokinetic features of YCZFD are helpful for understanding the material bases of its therapeutic effects.
Department of Pharmacology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Qian
Q
Wang
Department of Pharmacology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Peng
P
Jiang
Instrumental Analysis Center, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Fu-Yuan
FY
Ye
Department of Pharmacology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Rong
R
Shi
Department of Pharmacology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. Electronic address: mayueming_117@hotmail.com.
Yue-Ming
YM
Ma
Department of Pharmacology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Jie
J
Zhong
Department of Pharmacology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Jia-Sheng
JS
Wu
Key Laboratory of Liver and Kidney Diseases (Ministry of Education), Institute of Liver Diseases, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Ping
P
Liu
Key Laboratory of Liver and Kidney Diseases (Ministry of Education), Institute of Liver Diseases, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Cheng-Hai
CH
Liu
Instrumental Analysis Center, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Yi-Qun
YQ
Jia
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000737:chemistry* / Q000493:pharmacokinetics*; D005260:Female; D008297:Male; D064209:Phytochemicals / Q000302:isolation & purification / Q000493:pharmacokinetics; D017207:Rats, Sprague-Dawley; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
D004365:Drugs, Chinese Herbal; D064209:Phytochemicals; C000594294:yinchenzhufu
Database-directed strategy; HRMS; Multiple constituents; Pharmacokinetics; UPLC-MS/MS; Yinchenzhufu decoction