Interleukins affect equine endometrial cell function: modulatory action of ovarian steroids.
The aim of the present study was to investigate the interaction between ovarian steroids, interleukins and prostaglandins (PG) in equine epithelial and stromal cells in vitro. In Experiment 1, cells were exposed to IL-1α (10 ng/mL), IL-1β (10 ng/mL) or IL-6 (10 ng/mL) for 24 h and cell proliferation was determined using MTT. In Experiment 2, cells were exposed to progesterone (P4; 10(-7) M); 17-β estradiol (E2; 10(-9) M) or P4+E2 for 24 h and later medium was replaced with a fresh one treated with IL-1α, IL-1β or IL-6 (10 ng/mL, each) for 24 h. The oxytocin (OT; 10(-7) M) was used as a positive control. In Experiment 3, cells were exposed to P4 (10(-7) M), E2 (10(-9) M) or P4+E2 for 24 h and the IL receptor mRNAs transcription was determined using Real-time PCR. Prostaglandins concentration was determined using the direct enzyme immunoassay (EIA) method. Our findings reveal a functional linking between ovarian steroids and IL-stimulated PG secretion by equine endometrial cells. This interaction could be one of the mechanisms responsible for endometrial local orchestrating events during the estrous cycle and early pregnancy.
Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, 10-748 Olsztyn, Poland.
Anna Z
AZ
Szóstek
Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, 10-748 Olsztyn, Poland.
Antonio M
AM
Galvão
Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, 10-748 Olsztyn, Poland.
Takuo
T
Hojo
Laboratory of Reproductive Endocrinology Graduate School of Natural Science and Technology, Okayama University, 700-8530 Okayama, Japan.
Kiyoshi
K
Okuda
Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, 10-748 Olsztyn, Poland.
Dariusz J
DJ
Skarzynski
D000818:Animals; D049109:Cell Proliferation; D002478:Cells, Cultured; D003470:Culture Media; D004717:Endometrium / Q000166:cytology*; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D005786:Gene Expression Regulation; D006736:Horses; D007124:Immunoenzyme Techniques; D053582:Interleukin-1alpha / Q000494:pharmacology; D053583:Interleukin-1beta / Q000494:pharmacology; D015850:Interleukin-6 / Q000494:pharmacology; D007378:Interleukins / Q000494:pharmacology*; D010053:Ovary / Q000378:metabolism*; D011374:Progesterone / Q000494:pharmacology; D011453:Prostaglandins / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D013256:Steroids / Q000378:metabolism*
D003470:Culture Media; D053582:Interleukin-1alpha; D053583:Interleukin-1beta; D015850:Interleukin-6; D007378:Interleukins; D011453:Prostaglandins; D012333:RNA, Messenger; D013256:Steroids; D011374:Progesterone; D004958:Estradiol