Comparing and visualizing titanium implant integration in rat bone using 2D and 3D techniques.
The aim was to compare the osseointegration of grit-blasted implants with and without a hydrogen fluoride treatment in rat tibia and femur, and to visualize bone formation using state-of-the-art 3D visualization techniques. Grit-blasted implants were inserted in femur and tibia of 10 Sprague-Dawley rats (4 implants/rat). Four weeks after insertion, bone implant samples were retrieved. Selected samples were imaged in 3D using Synchrotron Radiation-based μCT (SRμCT). The 3D data was quantified and visualized using two novel visualization techniques, thread fly-through and 2D unfolding. All samples were processed to cut and ground sections and 2D histomorphometrical comparisons of bone implant contact (BIC), bone area (BA), and mirror image area (MI) were performed. BA values were statistically significantly higher for test implants than controls (p < 0.05), but BIC and MI data did not differ significantly. Thus, the results partly indicate improved bone formation at blasted and hydrogen fluoride treated implants, compared to blasted implants. The 3D analysis was a valuable complement to 2D analysis, facilitating improved visualization. However, further studies are required to evaluate aspects of 3D quantitative techniques, with relation to light microscopy that traditionally is used for osseointegration studies.
Dentsply IH AB, Box 14, SE 431 21 Mölndal, Sweden; University of Gothenburg, The Sahlgrenska Academy, Institute of Odontology, Department of Prosthodontics/Dental Materials Science., P.O. Box 450, SE 405 30 Göteborg, Sweden.
Anna
A
Arvidsson
Hamid
H
Sarve
Carina B
CB
Johansson
D000818:Animals; D001863:Bone Screws / Q000009:adverse effects*; D020099:Coated Materials, Biocompatible / Q000009:adverse effects*; D005269:Femur / Q000648:ultrastructure*; D021621:Imaging, Three-Dimensional; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013977:Tibia / Q000648:ultrastructure*; D014025:Titanium / Q000009:adverse effects*
D020099:Coated Materials, Biocompatible; D014025:Titanium
SRµCT; histomorphometry; osseointegration; rat model; titanium