S phase cell percentage normalized BrdU incorporation rate, a new parameter for determining S arrest.
In this study, a new parameter, S phase cell percentage (S fraction) normalized BrdU (SFN-BrdU) incorporation rate, was introduced to detect S arrest. The results showed a positive linear correlation between the BrdU incorporation rate and the S fraction in unperturbed 16HBE cells. Theoretical analysis indicated that only S arrest could result in a decrease in the SFN-BrdU incorporation rate. Additionally, the decrease in SFN-BrdU incorporation rate and the activation of DNA damage checkpoints further demonstrated that S arrest was induced by diethyl sulfate treatment of 16HBE cells. In conclusion, SFN-BrdU incorporation rate can be used to detecting S arrest.
Department of Toxicology, Peking University Health Science Center, Beijing 100191, China; Beijing Key Laboratory of Toxicological Research and Risk Assessment for Food Safety, Peking University Health Science Center, Beijing 100191, China.
Peng
P
Zhao
Department of Toxicology, Peking University Health Science Center, Beijing 100191, China.
Juan Ling
JL
Fu
Department of Toxicology, Peking University Health Science Center, Beijing 100191, China; Beijing Key Laboratory of Toxicological Research and Risk Assessment for Food Safety, Peking University Health Science Center, Beijing 100191, China.
Bi Yun
BY
Yao
Medical and Healthy Analytical Center, Peking University Health Science Center, Beijing 100191, China.
Yong Rui
YR
Jia
Department of Toxicology, Peking University Health Science Center, Beijing 100191, China.
Zong Can
ZC
Zhou
D001973:Bromodeoxyuridine / Q000493:pharmacokinetics*; D049109:Cell Proliferation; D004249:DNA Damage; D004847:Epithelial Cells / Q000166:cytology*; D006801:Humans; D016196:S Phase; D059807:S Phase Cell Cycle Checkpoints
D001973:Bromodeoxyuridine