Jellyfish skin polysaccharides: extraction and inhibitory activity on macrophage-derived foam cell formation.
In this work, response surface methodology was used to determine optimum conditions for extraction of polysaccharides from jellyfish skin (JSP). The optimum parameters were found to be raw material to water ratio 1:7.5 (w/v), extraction temperature 100°C and extraction time 4h. Under these conditions, the JSP yield reached 1.007 mg/g. Papain (15 U/mL) in combination with Sevag reagent was beneficial in removing proteins from JSP. After precipitation with ethanol at final concentration of 40%, 60% and 80% in turn, three polysaccharide fractions of JSP1, JSP2 and JSP3 were obtained from JSP, respectively. The three fractions exhibited different physicochemical properties with respect to molecular weight distribution, monosaccharide composition, infrared absorption spectra, and glycosyl bond composition. In addition, JSP3 showed strong inhibitory effects on oxidized low-density lipoprotein (oxLDL) induced conversion of macrophages into foam cells, which possibly attributed to the down-regulation of some atherogenesis-related gene expressions.
School of Biotechnology and Food Engineering, Hefei University of Technology, No. 193 Tunxi Road, Hefei 230009, People's Republic of China.
Hai-Lin
HL
Zhang
School of Biotechnology and Food Engineering, Hefei University of Technology, No. 193 Tunxi Road, Hefei 230009, People's Republic of China.
Shao-Hua
SH
Cui
School of Biotechnology and Food Engineering, Hefei University of Technology, No. 193 Tunxi Road, Hefei 230009, People's Republic of China. Electronic address: zhaxueqiang@hfut.edu.cn.
Xue-Qiang
XQ
Zha
Department of Chemistry, University of Puerto Rico at Cayey, No. 225 Antonio R. Barcelo Avenue, Cayey 00736-9997, Puerto Rico. Electronic address: vibha.bansal@upr.edu.
Vibha
V
Bansal
School of Biotechnology and Food Engineering, Hefei University of Technology, No. 193 Tunxi Road, Hefei 230009, People's Republic of China.
Lei
L
Xue
School of Biotechnology and Food Engineering, Hefei University of Technology, No. 193 Tunxi Road, Hefei 230009, People's Republic of China.
Xiao-Long
XL
Li
School of Biotechnology and Food Engineering, Hefei University of Technology, No. 193 Tunxi Road, Hefei 230009, People's Republic of China.
Ran
R
Hao
School of Biotechnology and Food Engineering, Hefei University of Technology, No. 193 Tunxi Road, Hefei 230009, People's Republic of China.
Li-Hua
LH
Pan
School of Biotechnology and Food Engineering, Hefei University of Technology, No. 193 Tunxi Road, Hefei 230009, People's Republic of China. Electronic address: jianpingluo@hfut.edu.cn.
Jian-Ping
JP
Luo
D020001:1-Butanol / Q000494:pharmacology; D000818:Animals; D050197:Atherosclerosis / Q000235:genetics; D002460:Cell Line; D011232:Chemical Precipitation; D002725:Chloroform / Q000494:pharmacology; D002784:Cholesterol / Q000032:analysis; D015536:Down-Regulation / Q000187:drug effects; D004353:Drug Evaluation, Preclinical; D000431:Ethanol / Q000494:pharmacology; D005487:Foam Cells / Q000737:chemistry / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D008077:Lipoproteins, LDL / Q000494:pharmacology; D008264:Macrophages / Q000737:chemistry / Q000187:drug effects*; D051379:Mice; D008970:Molecular Weight; D010206:Papain / Q000494:pharmacology; D011134:Polysaccharides / Q000302:isolation & purification* / Q000494:pharmacology; D060888:Real-Time Polymerase Chain Reaction; D037401:Scyphozoa / Q000737:chemistry*; D012997:Solvents / Q000494:pharmacology; D013055:Spectrophotometry, Infrared
D008077:Lipoproteins, LDL; D011134:Polysaccharides; D012997:Solvents; C099252:oxidized low density lipoprotein; D000431:Ethanol; D002725:Chloroform; D020001:1-Butanol; D002784:Cholesterol; D010206:Papain
Atherosclerosis; Foam cell; Jellyfish; Polysaccharide; Rhopilema esculetum