CD4+FOXP3+ cells produce IL-10 in the spleens of dogs with visceral leishmaniasis.
Visceral Leishmaniasis (VL) is caused by intracellular parasites of the genus Leishmania that affect humans and several animal species. Dogs are one of the main urban reservoirs of the parasite and play a central role in the transmission cycle to humans via sandflies. Studies concerning the immune response in dogs with VL have demonstrated that protective immunity is associated with cellular immune response, while disease progression is associated with humoral response and IL-10 and TGF-β production. The study aimed to evaluate IL-10 and TGF-β production by regulatory T (Treg) cells in the blood and spleen of dogs naturally infected by Leishmania spp. and correlate this with parasite load. Five healthy dogs and 29 dogs with proven infection were selected for the study group. Real-time PCR was used to quantify parasite load and confirm infection by Leishmania spp. Treg cells producing IL-10 and TGF-β were quantified using flow cytometry. An increase in IL-10 production by Treg cells was verified in the spleen of dogs naturally infected by Leishmania spp. Concurrently, a decrease in the total number of T cells in these dogs was verified compared with healthy dogs. No association was determined between parasite load and the percentage of spleen Treg cells producing IL-10 and TGF-β. These findings suggest that Treg cells are an important source of IL-10 in the spleen, participating in immune response modulation, while the reduced percentage of these cells in infected dogs could be attributed to persistent immune activation.
Faculty of Veterinary Medicine, São Paulo State University (UNESP), Araçatuba, SP, Brazil.
Kathlenn Liezbeth Oliveira
KL
Silva
Faculty of Veterinary Medicine, São Paulo State University (UNESP), Araçatuba, SP, Brazil.
Mariana M C
MM
de Andrade
Faculty of Veterinary Medicine, São Paulo State University (UNESP), Araçatuba, SP, Brazil.
Larissa M
LM
Melo
Faculty of Veterinary Medicine, São Paulo State University (UNESP), Araçatuba, SP, Brazil.
Juliana
J
Perosso
Department of Veterinary Pathology, Faculty of Agricultural and Veterinary Sciences, São Paulo State University (UNESP), Jaboticabal, SP, Brazil.
Rosemeri O
RO
Vasconcelos
Department of Exact Sciences, Faculty of Agricultural and Veterinary Sciences, São Paulo State University (UNESP), Jaboticabal, SP, Brazil.
Danisio P
DP
Munari
Department of Clinical Surgery and Animal Reproduction, Faculty of Veterinary Medicine, São Paulo State University (UNESP), Araçatuba, SP, Brazil. Electronic address: vmflima@fmva.unesp.br.
Valéria M F
VM
Lima
D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D004283:Dog Diseases / Q000097:blood / Q000276:immunology*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D051858:Forkhead Transcription Factors / Q000378:metabolism*; D016753:Interleukin-10 / Q000378:metabolism*; D007898:Leishmaniasis, Visceral / Q000276:immunology / Q000662:veterinary*; D008297:Male; D059208:Parasite Load; D013154:Spleen / Q000276:immunology* / Q000469:parasitology; D016212:Transforming Growth Factor beta / Q000378:metabolism
D051858:Forkhead Transcription Factors; D016212:Transforming Growth Factor beta; D016753:Interleukin-10
Dog cytokines; IL-10; Leishmania; Regulatory T cells; TGF-β