Low dose of propranolol does not affect rat osteotomy healing and callus strength.
Experimental studies suggest that the β-blocker propranolol stimulates bone formation but little work has investigated its effect on fracture healing. In this study, we examined if a low dose of propranolol, previously shown to be preventive against bone loss in rats, improves bone repair. Female Wistar rats were injected with saline or propranolol (0.1 mg/kg/day) (n = 20/group), 5 days a week for 8 weeks. Three weeks after the beginning of treatment, all rats underwent a mid-diaphyseal transverse osteotomy in the left femur. Radiographic analysis of ostetomy healing was performed 2 and 5 weeks after osteotomy. Rats were sacrificed at 5 weeks and femora collected for measurements of fracture strength by torsional testing, callus volume, and mineral content by micro-CT analysis and histology of fracture callus. Eighty nine percent of osteotomies achieved apparent radiological union by 5 weeks in both groups. Propranolol treatment did not significantly alter the torsional strength of the fractured femur compared with controls. The volume and mineralization of fracture callus at 5 weeks were not significantly different in both groups. Histology showed that endochondral ossification was not affected by propranolol. Altogether, our results demonstrate that propranolol using the regimen described does not significantly improve or inhibit rat osteotomy healing and mechanical strength.
Institute of Orthopaedics and Musculoskeletal Science, UCL, Royal National Orthopaedic Hospital, London, Stanmore, United Kingdom.
Peter
P
Smitham
Lawrence
L
Crossfield
Gillian
G
Hughes
Allen
A
Goodship
Gordon
G
Blunn
Chantal
C
Chenu
D000319:Adrenergic beta-Antagonists / Q000008:administration & dosage*; D000818:Animals; D001696:Biomechanical Phenomena; D001842:Bone and Bones / Q000187:drug effects*; D002146:Bony Callus / Q000187:drug effects / Q000503:physiopathology*; D005260:Female; D005264:Femoral Fractures / Q000000981:diagnostic imaging / Q000601:surgery; D005269:Femur / Q000000981:diagnostic imaging / Q000473:pathology; D017102:Fracture Healing / Q000187:drug effects*; D006801:Humans; D010012:Osteogenesis; D010027:Osteotomy; D011433:Propranolol / Q000008:administration & dosage*; D051381:Rats; D017208:Rats, Wistar; D018343:Receptors, Adrenergic, beta-2 / Q000378:metabolism; D013314:Stress, Mechanical; D013997:Time Factors; D055114:X-Ray Microtomography
D000319:Adrenergic beta-Antagonists; D018343:Receptors, Adrenergic, beta-2; D011433:Propranolol
bone architecture; fracture healing; mechanical testing; micro-CT; propranolol