Vitamin C treatment of mouse bone marrow-derived dendritic cells enhanced CD8(+) memory T cell production capacity of these cells in vivo.
Vitamin C has been found to stimulate dendritic cells (DCs) to secrete more IL-12 and thereby drive naïve CD4(+) T cells to differentiate into Th1 cells. In the present study, we evaluated the effect of these vitamin C-treated DCs on CD8(+) T cell differentiation both in vitro and in vivo. Mouse bone marrow-derived DCs were prepared in the presence of GM-CSF and IL-15. With vitamin C treatment, these DCs, when LPS-stimulated, secreted more IL-12p70 and IL-15 than did untreated DCs. And when co-cultured with T cells, they yielded a higher frequency of IFN-γ(+) CD8(+) T cells. Moreover, we found that administering vitamin C-treated and tumor lysate-loaded DCs into mice yielded a higher frequency of CD44(high) CD62L(low) CD8(+) effector and effector memory T cells, which showed an increased ex vivo killing effect of the tumor cells. These DCs also elicited enhanced protective effects against inoculated tumor cells, most probably by way of the increased cytotoxic T cells, as was revealed by the decreased growth of the inoculated tumor cells in these mice. This ex vivo vitamin C treatment effect on DCs can be considered as a strategy for boosting DC vaccination potency against tumors.
Department of Anatomy, Seoul National University College of Medicine, Seoul 110-799, Republic of Korea.
Young-Joo
YJ
Jeong
Department of Anatomy, Seoul National University College of Medicine, Seoul 110-799, Republic of Korea.
Jin-Hee
JH
Kim
Department of Anatomy, Seoul National University College of Medicine, Seoul 110-799, Republic of Korea.
Jun-Man
JM
Hong
Department of Anatomy, Seoul National University College of Medicine, Seoul 110-799, Republic of Korea.
Jae Seung
JS
Kang
Department of Anatomy, Seoul National University College of Medicine, Seoul 110-799, Republic of Korea.
Hang-Rae
HR
Kim
Department of Anatomy, Seoul National University College of Medicine, Seoul 110-799, Republic of Korea.
Wang Jae
WJ
Lee
Department of Anatomy, Seoul National University College of Medicine, Seoul 110-799, Republic of Korea. Electronic address: Hyi830@snu.ac.kr.
Young-il
YI
Hwang
D000818:Animals; D001205:Ascorbic Acid / Q000276:immunology* / Q000494:pharmacology; D001854:Bone Marrow Cells / Q000187:drug effects / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology* / Q000637:transplantation; D005434:Flow Cytometry; D018960:Hyaluronan Receptors / Q000276:immunology / Q000378:metabolism; D007156:Immunologic Memory / Q000276:immunology*; D016219:Immunotherapy, Adoptive / Q000379:methods; D007371:Interferon-gamma / Q000276:immunology / Q000378:metabolism; D018664:Interleukin-12 / Q000276:immunology / Q000378:metabolism; D019409:Interleukin-15 / Q000276:immunology / Q000378:metabolism; D019041:L-Selectin / Q000276:immunology / Q000378:metabolism; D008070:Lipopolysaccharides / Q000276:immunology / Q000494:pharmacology; D008297:Male; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009374:Neoplasms, Experimental / Q000276:immunology / Q000473:pathology / Q000628:therapy; D016019:Survival Analysis; D014611:Vaccination / Q000379:methods; D014815:Vitamins / Q000276:immunology / Q000494:pharmacology
D018960:Hyaluronan Receptors; D019409:Interleukin-15; D008070:Lipopolysaccharides; D014815:Vitamins; D019041:L-Selectin; D018664:Interleukin-12; D007371:Interferon-gamma; D001205:Ascorbic Acid
CD8(+) T cells; Dendritic cell vaccination; Dendritic cells; Memory cell differentiation; Vitamin C