Cell-based assay system to estimate the effect of 125I seeds on cancer cells: effect of osteopontin.
OBJECTIVE
A number of recent patents related to 125I seed and osteopontin have been awarded. In this study, we developed a new in vitro model to study the radiobiological effects of 125I seeds on cancer cells.


METHODS
We placed 125I seeds in a cell culture plate with cells seeded on a transwell chamber to evaluate the effects of 125I seeds on cell motility. We placed 125I seeds in a transwell chamber with cells seeded on the cell culture plate to study cell proliferation, apoptosis, and to perform western blot.


RESULTS
We used SK-Hep-1 liver cancer cells to evaluate the effectiveness of our model, and investigated the role of osteopontin in the therapeutic effects of 125I seeds. We found that 125I seeds inhibited the cell proliferation and motility in this model while osteopontin reduced these effects. We also measured the effects of 125I seeds on apoptosis. Furthermore, when treated with 125I seeds, we detected changes in several signaling pathways that are involved in the proliferation and invasion of cancer cells.


CONCLUSIONS
The model can be used to assess the biological effects of 125I seeds in vitro. Osteopontin may be involved in the 125I seed-induced inhibition of SK-Hep-1 cells.
Guang
G
Yang
Sheng
S
Peng
Yanling
Y
Zhang
Zhenyin
Z
Liu
Mingjian
M
Lu
Tao
T
Zhang
Fei
F
Gao
State Key Laboratory of Oncology in South China, Department of Imaging and Interventional Radiology, Cancer Center, Sun Yat-Sen University, Guangzhou, Guangdong 510060, P.R. China. zhangfj@sysucc.org.cn.
Fujun
F
Zhang
D017209:Apoptosis / Q000187:drug effects* / Q000528:radiation effects; D001918:Brachytherapy; D006528:Carcinoma, Hepatocellular; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects* / Q000528:radiation effects; D049109:Cell Proliferation / Q000187:drug effects* / Q000528:radiation effects; D006801:Humans; D066298:In Vitro Techniques; D007457:Iodine Radioisotopes / Q000494:pharmacology*; D008113:Liver Neoplasms; D053495:Osteopontin / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects
D007457:Iodine Radioisotopes; D053495:Osteopontin