How air influences radiation dose deposition in multiwell culture plates: a Monte Carlo simulation of radiation geometry.
Radiation of experimental culture cells on plates with various wells can cause a risk of underdosage as a result of the existence of multiple air-water interfaces. The objective of our study was to quantify this error in culture plates with multiple wells. Radiation conditions were simulated with the GAMOS code, based on the GEANT4 code, and this was compared with a simulation performed with PENELOPE and measured data. We observed a slight underdosage of ∼ 4% on the most superficial half of the culture medium. We believe that this underdosage does not have a significant effect on the dose received by culture cells deposited in a monolayer and adhered to the base of the wells.
Department of Radiation Oncology, Complejo Hospitalario Universitario de Albacete (CHUA), C/ Hnos Falcó 37, 02006 Albacete, Spain ssabaterm@gmail.com.
Sebastia
S
Sabater
Department of Medical Physics, Complejo Hospitalario Universitario de Albacete (CHUA), C/ Hnos Falcó 37, 02006 Albacete, Spain.
Roberto
R
Berenguer
Centro Regional de Investigaciones Biomedicas (CRIB), Universidad de Castilla-la Mancha (UM), C/ Almansa 14, 02006 Albacete, Spain.
Paloma
P
Honrubia-Gomez
Department of Medical Physics, Complejo Hospitalario Universitario de Albacete (CHUA), C/ Hnos Falcó 37, 02006 Albacete, Spain.
Miguel
M
Rivera
Department of Medical Physics, Complejo Hospitalario Universitario de Albacete (CHUA), C/ Hnos Falcó 37, 02006 Albacete, Spain.
Ana
A
Nuñez
Department of Radiation Oncology, Hospital Son Espases, Carretera de Valldemossa 79, 07120 Palma de Mallorca, Spain.
Esther
E
Jimenez-Jimenez
Department of Radiation Oncology, Complejo Hospitalario Universitario de Albacete (CHUA), C/ Hnos Falcó 37, 02006 Albacete, Spain.
Ana
A
Martos
Centro Regional de Investigaciones Biomedicas (CRIB), Universidad de Castilla-la Mancha (UM), C/ Almansa 14, 02006 Albacete, Spain Instituto de Salud Carlos III. Av Monforte de Lemos 5, 28029 Madrid, Spain.
Carmen
C
Ramirez-Castillejo
D065638:Absorption, Radiation; D000388:Air; D000818:Animals; D018929:Cell Culture Techniques / Q000295:instrumentation*; D002468:Cell Physiological Phenomena / Q000528:radiation effects*; D003198:Computer Simulation; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D015233:Models, Statistical; D009010:Monte Carlo Method; D012542:Scattering, Radiation
Monte Carlo; culture cells; plate irradiation