Comparisons of responses by planarian to micromolar to attomolar dosages of morphine or naloxone and/or weak pulsed magnetic fields: revealing receptor subtype affinities and non-specific effects.
OBJECTIVE
The behavioral responses of planaria to the exposures of a range of concentrations of morphine (μM to attoM) or the μ-opiate antagonist naloxone or to either of these compounds and a burst-firing magnetic field (5 μT) were studied.


METHODS
The locomotor velocity (LMV) of planaria was measured after individual worms were exposed to increasing concentrations from attomolar to micromolar of morphine or naloxone, physiologically patterned magnetic fields or a combination of the two.


RESULTS
Compared to spring water controls, the 2-hour exposure to the patterned magnetic field before measurement reduced activity by about 50% which was comparable to the non-specific effects of morphine and naloxone across all dosages except 1 attomolar that did not differ from spring water. The specific dosage of 100 nM produced additional marked reduction in activity for planaria exposed to either morphine or naloxone while only 1 pM of morphine produced this effect.


CONCLUSIONS
The results support the presence of at least two receptor subtypes that mediate the diminished activity effects elicited by morphine specifically and suggests that exposure to the specifically patterned magnetic field produces a behavioral suppression whose magnitude is similar to the 'dose independent' effects from this opiate.
Department of Biomolecular Sciences and Behavioural Neuroscience Program, Laurentian University , Sudbury, Ontario , Canada.
Nirosha J
NJ
Murugan
Michael A
MA
Persinger
D000701:Analgesics, Opioid / Q000008:administration & dosage; D000818:Animals; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D008124:Locomotion / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D060526:Magnetic Fields; D009020:Morphine / Q000008:administration & dosage*; D009043:Motor Activity / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D009270:Naloxone / Q000008:administration & dosage*; D009292:Narcotic Antagonists / Q000008:administration & dosage; D010932:Planarians / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D011829:Radiation Dosage; D011957:Receptors, Opioid / Q000819:agonists / Q000378:metabolism*
D000701:Analgesics, Opioid; D009292:Narcotic Antagonists; D011957:Receptors, Opioid; D009270:Naloxone; D009020:Morphine
Planaria; morphine; naloxone; receptor subtypes; weak analgesic magnetic fields