Efficacy of a novel topical fipronil, (S)-methoprene, eprinomectin and praziquantel combination against naturally acquired intestinal nematode and cestode infections in cats.
The efficacy of a novel topical combination formulation of fipronil, (S)-methoprene, eprinomectin and praziquantel against naturally acquired intestinal nematode and cestode infections in cats was evaluated in seven negative control, blinded studies. Cats were selected based on a pre-treatment faecal examination indicating a patent infection with at least hookworms (two studies), Toxocara ascarids (one study), taeniid cestodes (two studies) or Dipylidium cestodes (two studies). In each study, cats were assigned randomly to blocks of two animals each, based on decreasing pre-treatment body weight and were randomly allocated to one of two groups of six to 12 cats: untreated (control) or treated with topical fipronil (8.3%, w/v), (S)-methoprene (10%, w/v), eprinomectin (0.4%, w/v) and praziquantel (8.3%, w/v) (BROADLINE(®), Merial) at 0.12 mL/kg body weight (providing a minimum of 10mg fipronil+12 mg S-methoprene+0.5mg eprinomectin+10mg praziquantel per kg body weight). The topical treatment was administered directly on the skin in the midline of the neck in a single spot once on Day 0. For parasite recovery and count, cats were euthanized humanely and necropsied seven or ten days after treatment. A single treatment with the novel topical combination product provided 91% efficacy against Ancylostoma braziliense, ≥ 99% efficacy against Ancylostoma tubaeforme, and >97% efficacy against Toxocara cati. Similarly, excellent efficacy was established against Taenia taeniaeformis, Dipylidium caninum and Diplopylidium spp. as demonstrated by >97% and up to 100% reductions of cestode counts in the treated cats when compared to the untreated controls (P<0.01). All cats accepted the treatment well based on health observations post-treatment and daily health observations. No adverse experiences or other health problems were observed throughout the studies. The results of this series of controlled studies demonstrated high efficacy and excellent acceptability of the novel topical combination formulation of fipronil, (S)-methoprene, eprinomectin and praziquantel against a broad range of feline intestinal nematode and cestode infections.
Merial GmbH, Kathrinenhof Research Center, 83101 Rohrdorf, Germany. Electronic address: martin.knaus@merial.com.
Martin
M
Knaus
Qatar University, Department of Health Sciences, College of Arts and Sciences, Doha, Qatar.
Marawan A
MA
Abu-Madi
Universidad Nacional Autónoma de México, Coyoacán CP 04510, México D.F., Mexico.
Froylán
F
Ibarra-Velarde
ClinVet International (Pty) Ltd., 9321 Bloemfontein, South Africa.
Dawie J
DJ
Kok
Universiteti Bujqësor, Fakulteti i Mjeksise Veterinare, Kodër Kamëz, Tirana, Albania.
Ilir
I
Kusi
Universiteti Bujqësor, Fakulteti i Mjeksise Veterinare, Kodër Kamëz, Tirana, Albania.
Rezart
R
Postoli
Merial Limited, Duluth, GA 30096, USA.
S Theodore
ST
Chester
Merial Limited, Duluth, GA 30096, USA.
Joseph
J
Rosentel
Merial Limited, Athens, GA 30601, USA.
Roberto
R
Alva
Merial Limited, Athens, GA 30601, USA.
Jennifer
J
Irwin
Merial GmbH, Kathrinenhof Research Center, 83101 Rohrdorf, Germany.
Martin
M
Visser
Merial GmbH, Kathrinenhof Research Center, 83101 Rohrdorf, Germany.
Renate
R
Winter
Merial GmbH, Kathrinenhof Research Center, 83101 Rohrdorf, Germany.
Steffen
S
Rehbein
D000818:Animals; D000977:Antiparasitic Agents / Q000008:administration & dosage*; D002371:Cat Diseases / Q000188:drug therapy*; D002415:Cats; D002589:Cestoda; D002590:Cestode Infections / Q000188:drug therapy / Q000662:veterinary*; D004338:Drug Combinations; D005260:Female; D007411:Intestinal Diseases, Parasitic / Q000188:drug therapy / Q000662:veterinary*; D007559:Ivermectin / Q000008:administration & dosage / Q000031:analogs & derivatives*; D008297:Male; D008726:Methoprene / Q000008:administration & dosage; D009348:Nematoda; D009349:Nematode Infections / Q000188:drug therapy / Q000662:veterinary*; D011223:Praziquantel / Q000008:administration & dosage; D011720:Pyrazoles / Q000008:administration & dosage; D011897:Random Allocation; D016896:Treatment Outcome
D000977:Antiparasitic Agents; D004338:Drug Combinations; D011720:Pyrazoles; D011223:Praziquantel; D007559:Ivermectin; C101434:eprinomectin; D008726:Methoprene; C082360:fipronil
Ancylostoma braziliense; Ancylostoma tubaeforme; Diplopylidium spp.; Dipylidium caninum; Taenia taeniaeformis; Toxocara cati