Comparison of anesthesia protocols for intravenous glucose tolerance testing in rhesus monkeys.
BACKGROUND
Drugs commonly used to sedate non-human primates for physiological sample collection can affect the metabolic system and alter rates of glucose metabolism. This study was designed to compare the physiological and metabolic effects of ketamine/diazepam, telazol, and ketamine/dexmedetomidine.


METHODS
Seven female rhesus monkeys underwent intravenous glucose tolerance testing under each of three anesthesia conditions. Blood glucose, insulin, physiological parameters, and sedation characteristics were measured and recorded.


RESULTS
Glucose and insulin values were both significantly impacted by ketamine/dexmedetomidine sedation while remaining consistent during ketamine and telazol sedation. Heart rate was also significantly lowered during ketamine/dexmedetomidine anesthesia. Though, ketamine/dexmedetomidine resulted in a longer time between induction of anesthesia and need for a supplemental dose of anesthesia drug.


CONCLUSIONS
Telazol and ketamine have minimal cardiorespiratory and metabolic effects compared to ketamine/dexmedetomidine. Although practicably interchangeable, telazol appears to be the most efficient for intravenous glucose tolerance testings with non-human primates.
Translational Gerontology Branch, Intramural Research Program, National Institute on Aging, NIH, Dickerson, MD, USA; SoBran, Inc., Burtonsville, MD, USA.
Kelli L
KL
Vaughan
Mark D
MD
Szarowicz
Richard L
RL
Herbert
Julie A
JA
Mattison
D000758:Anesthesia / Q000379:methods*; D000777:Anesthetics / Q000494:pharmacology*; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects*; D020927:Dexmedetomidine / Q000494:pharmacology; D003975:Diazepam / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D005260:Female; D005951:Glucose Tolerance Test; D006339:Heart Rate / Q000187:drug effects*; D007649:Ketamine / Q000494:pharmacology; D008253:Macaca mulatta / Q000378:metabolism*; D056152:Respiratory Rate / Q000187:drug effects*; D013992:Tiletamine / Q000494:pharmacology; D015041:Zolazepam / Q000494:pharmacology
D000777:Anesthetics; D004338:Drug Combinations; C006131:tiletamine, zolazepam drug combination; D013992:Tiletamine; D020927:Dexmedetomidine; D007649:Ketamine; D015041:Zolazepam; D003975:Diazepam
IVGTT; glucose regulation; non-human primate; pharmacology; rhesus macaque