Tissue factor and tissue factor pathway inhibitor in chronically inflamed gallbladder mucosa.
We characterised a tissue factor (TF) and tissue factor pathway inhibitor (TFPI) expression in relation to severity of inflammatory infiltration of the gallbladder mucosa in a chronic cholecystitis. We prospectively studied the gallbladder specimens obtained from 54 patients who had undergone cholecystectomy due to chronic calculous cholecystitis and 16 calculosis-free gallbladder specimens obtained from patients who underwent cholecystectomy due to the polyp/polyps as well as in cases of gallbladder injury. To assess TF and TFPI immunoreactivity by immunohistochemistry, the monoclonal anti-human TF and TFPI antibodies were used. The inflammatory infiltration of the gallbladder mucosa was reflected by the number of CD3 and CD68 positive cells. The expression of TF and TFPI differed significantly between the cholecystitis and the control group. Most capillary endothelial cells of the cholecystitis group presented weak expression for TFPI. The mean number of CD3 positive lymphocytes in the cholecystitis group was 18.6 ± 12.2, but the mean number of CD68 positive cells was 29.7 ± 13.9. In the control sections, it was 3.1 ± 1.9 and 8.8 ± 3.9, respectively (P < 0.001). The results of the current study suggest that the tissue procoagulant state found may be engaged in the etiopathogenesis of the cholecystitis.
Department of Histology and Embryology, Medical University of Silesia in Katowice, Jordana Street 19, 41-808 Zabrze, Poland.
Jacek
J
Liczko
Municipal Hospital, Chełmońskiego Street 28, 43-600 Jaworzno, Poland.
Tomasz
0000-0003-0093-4172
T
Stawski
Department of Histology and Embryology, Medical University of Silesia in Katowice, Jordana Street 19, 41-808 Zabrze, Poland.
Małgorzata
M
Zaba
Municipal Hospital, Chełmońskiego Street 28, 43-600 Jaworzno, Poland.
Józef
J
Kurek
Department of Pathomorphology, Medical University of Silesia in Katowice, 3-go Maja Street 13-15, 41-800 Zabrze, Poland.
Daniel
D
Sabat
Department of Histology and Embryology, Medical University of Silesia in Katowice, Jordana Street 19, 41-808 Zabrze, Poland.
Grzegorz
G
Wyrobiec
Department of Biochemistry, Medical University of Silesia in Katowice, Jedności Street 8, 41-200 Sosnowiec, Poland.
Dorota
D
Domal-Kwiatkowska
Department of Histology and Embryology, Medical University of Silesia in Katowice, Jordana Street 19, 41-808 Zabrze, Poland.
Damian
D
Dudek
Department of Descriptive and Topographic Anatomy, Medical University of Silesia in Katowice, Jordana Street 19, 41-808 Zabrze, Poland.
Marek
0000-0001-7950-679X
M
Kucharzewski
Department of Histology and Embryology, Medical University of Silesia in Katowice, Jordana Street 19, 41-808 Zabrze, Poland.
Krzysztof
K
Helewski
D015703:Antigens, CD / Q000378:metabolism; D015214:Antigens, Differentiation, Myelomonocytic / Q000378:metabolism; D017252:CD3 Complex / Q000378:metabolism; D005260:Female; D005704:Gallbladder / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D007249:Inflammation / Q000378:metabolism*; D008074:Lipoproteins / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009092:Mucous Membrane / Q000378:metabolism*; D011446:Prospective Studies; D013925:Thromboplastin / Q000378:metabolism*; D000097382:CD68 Molecule
D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D017252:CD3 Complex; D008074:Lipoproteins; D013925:Thromboplastin; C067980:CD68 antigen, human; D000097382:CD68 Molecule; C051928:lipoprotein-associated coagulation inhibitor