Citrus medica var. sarcodactylis (Foshou) activates fibroblast growth factor-2 signaling to induce migration of RSC96 Schwann cells.
Factors that enhance the intrinsic growth potential of neurons play a major role in the regeneration and repair of adult neurons following an injury. Fibroblast growth factor (FGF-2) is one of the key players in the origin and growth of neuronal and glial cells through autocrine and paracrine signaling. Water extract of Citrus medica var. sarcodactylis (fingered citron, foshou), which is been used effectively as a Chinese herbal medicine, was found to activate the FGF-2 promoter in transgenic luciferase expression models. Foshou treatment on Schwann cells (RSC96) transfected with luciferase reporter plasmid under a FGF-2 promoter was found to induce the FGF-2 promoter and showed enhanced luciferase expression. The FGF-2 expression was accompanied with an increase in the expression of proteins involved in cell migration and cell proliferation in a dose dependent manner. Therefore, foshou potentially enhances nerve regeneration by inducing the Schwann cell proliferation and migration.
Graduate Institute of Basic Medical Science, China Medical University, Taichung 40402, Taiwan , Graduate Institute of Chinese Medical Science, China Medical University, Taichung 40402, Taiwan , Department of Health and Nutrition Biotechnology, Asia University, Taichung 413, Taiwan.
Chih-Yang
CY
Huang
Wei-Wen
WW
Kuo
Marthandam Asokan
MA
Shibu
Ming-Fang
MF
Hsueh
Yueh-Sheng
YS
Chen
Fuu-Jen
FJ
Tsai
Chun-Hsu
CH
Yao
Chien-Chung
CC
Lin
Lung-Fa
LF
Pan
Da-Tong
DT
Ju
D000818:Animals; D019898:Autocrine Communication / Q000187:drug effects* / Q000235:genetics; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D002957:Citrus; D004305:Dose-Response Relationship, Drug; D016222:Fibroblast Growth Factor 2 / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D015870:Gene Expression / Q000235:genetics; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D009416:Nerve Regeneration / Q000187:drug effects* / Q000235:genetics; D019899:Paracrine Communication / Q000187:drug effects / Q000235:genetics; D010936:Plant Extracts / Q000494:pharmacology*; D011401:Promoter Regions, Genetic; D051381:Rats; D012583:Schwann Cells / Q000502:physiology*; D014162:Transfection; D014867:Water
D010936:Plant Extracts; D014867:Water; D016222:Fibroblast Growth Factor 2; D008156:Luciferases