Glucuronoarabinoxylan from coconut palm gum exudate: chemical structure and gastroprotective effect.
A glucuronoarabinoxylan (CNAL) was extracted with 1% aq. KOH (25°C) from Cocos nucifera gum exudate. It had a homogeneous profile on HPSEC-MALLS-RI (Mw 4.6 × 10(4)g/mol) and was composed of Fuc, Ara, Xyl, GlcpA (and 4-O-GlcpA) in a 7:28:62:3 molar ratio. Methylation data showed a branched structure with 39% of non-reducing end units, 3-O-substituted Araf (8%), 3,4-di-O- (15%), 2,4-di-O- (5%) and 2,3,4-tri-O-substituted Xylp units (17%). The anomeric region of CNAL (13)C NMR spectrum contained 9 signals, indicating a complex structure. The main chain of CNAL was characterized by analysis of a Smith-degraded polysaccharide. Its (13)C NMR spectrum showed 5 main signals at δ 101.6, δ 75.5, δ 73.9, δ 72.5, and δ 63.1 that were attributed to C-1, C-4, C-3, C-2 and C-5 of (1→4)-linked β-Xylp-main chain units, respectively. CNAL exhibited gastroprotective effect, by reducing gastric hemorrhagic lesions, when orally administered (1 and 3mg/kg) to rats prior to ethanol administration.
Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CP 19046, CEP 81531-990 Curitiba, PR, Brazil.
Fernanda F
FF
Simas-Tosin
Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CP 19046, CEP 81531-990 Curitiba, PR, Brazil.
Ruth R
RR
Barraza
Departamento de Farmacologia, Universidade Federal do Paraná, CP 19046, CEP 81531-990 Curitiba, PR, Brazil.
Daniele
D
Maria-Ferreira
Departamento de Farmacologia, Universidade Federal do Paraná, CP 19046, CEP 81531-990 Curitiba, PR, Brazil.
Maria Fernanda de P
MF
Werner
Departamento de Farmacologia, Universidade Federal do Paraná, CP 19046, CEP 81531-990 Curitiba, PR, Brazil.
Cristiane H
CH
Baggio
Departamento de Medicina Forense e Psiquiatria, Universidade Federal do Paraná, CP 19046, CEP 81531-990 Curitiba, PR, Brazil.
Ricardo
R
Wagner
Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CP 19046, CEP 81531-990 Curitiba, PR, Brazil.
Fhernanda R
FR
Smiderle
Departamento de Química, Universidade Federal de Goiás, CEP 75702-040 Catalão, GO, Brazil.
Elaine R
ER
Carbonero
Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CP 19046, CEP 81531-990 Curitiba, PR, Brazil.
Guilherme L
GL
Sassaki
Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CP 19046, CEP 81531-990 Curitiba, PR, Brazil. Electronic address: iacomini@ufpr.br.
Marcello
M
Iacomini
Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CP 19046, CEP 81531-990 Curitiba, PR, Brazil. Electronic address: cesarat@ufpr.br.
Philip A J
PA
Gorin
D000818:Animals; D003059:Cocos / Q000737:chemistry*; D019610:Cytoprotection / Q000187:drug effects*; D005260:Female; D008970:Molecular Weight; D053149:Plant Gums / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D013270:Stomach / Q000187:drug effects*; D014990:Xylans / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*
D053149:Plant Gums; D014990:Xylans; C114470:glucuronoarabinoxylan
Cocos nucifera; Gastroprotective effect; Glucuronoarabinoxylan; Gum exudate