Investigation of NF-κB1 and NF-κBIA gene polymorphism in non-small cell lung cancer.
Lung cancer is a complex, multifactorial disease which is the leading cause of cancer death in both men and women. NF-κB is a transcription factor which is known to affect the expression of more than 150 genes related to inflammation, lymphocyte activation, cell proliferation, differentiation, and apoptosis, as well as contributing to cell apoptosis and survival. However, NF-κBIA (IκBα) is the inhibitor of the transcription factor. The--94ins/delATTG polymorphism of the NF-κB1 gene promoter region which causes a functional effect and NF-κBIA 3'UTR A → G polymorphism has been shown to be related to various inflammatory diseases and cancer. Ninety-five NSCLC patients and 99 healthy controls were included in study. The NF-κB1-94ins/delATTG and NF-κBIA 3'UTR A → G polymorphism have been studied by using PCR-RFLP method. It was found that the NF-κB1 -94ins/delATTG DD genotype and D allele frequencies were higher in patients than healthy controls and the presence of the DD genotype has a 3.5-fold increased risk of the disease (P: 0.014). This study is the first to investigate the NF-κB1-94ins/delATTG and NF-κBIA 3'UTR A → G polymorphism together in the Turkish population. According to the results, the NF-κB1-94ins/del ATTG promoter polymorphism may have a role in lung carcinogenesis and prognosis.
Department of Molecular Medicine, Institute of Experimental Medicine Research, Istanbul University DETAE, P.O. Box 7, 34390 Çapa/İstanbul, Turkey.
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Oltulu
Department of Molecular Medicine, Institute of Experimental Medicine Research, Istanbul University DETAE, P.O. Box 7, 34390 Çapa/İstanbul, Turkey.
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Coskunpinar
Department of Molecular Medicine, Institute of Experimental Medicine Research, Istanbul University DETAE, P.O. Box 7, 34390 Çapa/İstanbul, Turkey.
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Ozkan
Department of Chest Diseases, Faculty of Medicine, Medipol University, Istanbul, Turkey.
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Aynaci
Third Clinics, Yedikule Chest Diseases and Thoracic Surgery Training Research Hospital, Istanbul, Turkey.
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Yildiz
Department of Medical Biology, School of Medicine, Yeditepe University, Istanbul, Turkey.
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Isbir
Department of Molecular Medicine, Institute of Experimental Medicine Research, Istanbul University DETAE, P.O. Box 7, 34390 Çapa/İstanbul, Turkey.
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Yaylim
D000368:Aged; D000483:Alleles; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000473:pathology; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D020672:I-kappa B Proteins / Q000235:genetics*; D007249:Inflammation / Q000235:genetics; D008297:Male; D008875:Middle Aged; D000072000:NF-KappaB Inhibitor alpha; D016328:NF-kappa B / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D011379:Prognosis; D011401:Promoter Regions, Genetic
D020672:I-kappa B Proteins; D016328:NF-kappa B; C000606204:NFKBIA protein, human; D000072000:NF-KappaB Inhibitor alpha