In vivo optogenetics for light-induced oxidative stress in transgenic zebrafish expressing the KillerRed photosensitizer protein.
Optogenetic methods are gaining broad recognition. The zebrafish is particularly useful for these applications as a model vertebrate due to a unique combination of translucent embryos/larvae and efficient transgenesis. Here, we describe a zebrafish model of light-induced cardiac deficiency. Upon illumination with intense green light, the membrane-tethered photosensitizer protein KillerRed acts as a photoinducer of reactive oxygen species which in turn cause changes in heart rate and contractility in hearts that express this transgene.
Institute of Molecular and Cell Biology, Agency for Science, Technology and Research, 61 Biopolis Drive, Proteos, Singapore, Singapore, 138673.
Cathleen
C
Teh
Vladimir
V
Korzh
D000818:Animals; D030801:Animals, Genetically Modified; D005260:Female; D015870:Gene Expression; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006339:Heart Rate; D007814:Larva / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects; D008027:Light; D008297:Male; D009200:Myocardial Contraction; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D062308:Optogenetics; D018384:Oxidative Stress / Q000528:radiation effects*; D017319:Photosensitizing Agents / Q000378:metabolism; D016276:Ventricular Function; D015027:Zebrafish / Q000235:genetics* / Q000378:metabolism
D017319:Photosensitizing Agents; C508771:killer red protein, Anthomedusae; D049452:Green Fluorescent Proteins