Neurobehavioral evaluation of adolescent male rats following repeated exposure to chlorpyrifos.
Chlorpyrifos (CPF), a highly effective organophosphate pesticide (OP), is extensively used worldwide. However, its agricultural use was extensively reduced in 2001. Several studies have suggested an association between mood disorders and CPF exposure in humans, especially in children, a subgroup that is highly susceptible to xenobiotics. We investigated the hypothesis that repeated CPF exposure in animals would elicit depressive-like behavioral alterations that reflected depression-related symptoms. Adolescent male rats were subcutaneously injected with either olive oil or 2.5, 5, 10, or 20mg/kg CPF from postnatal day 27 to 36, then were followed by a series of neurobehavioral evaluation. Our studies revealed depressive-like alterations that were manifested by increased despair behavior in the forced swimming test, increased escape failure in the learned helplessness test, and altered approach-avoidance conflict in the novelty-suppressed feeding test. There was no effect on locomotor activity in the open-field activity test. This study indicates that repeated exposure to CPF elicits depressive-like behavioral alterations in adolescent male rats.
Department of New Drug Evaluation, Beijing Institute of Pharmacology and Toxicology, 27 Taiping Road, Beijing 100850, PR China.
Wen-Qiang
WQ
Chen
Department of New Drug Evaluation, Beijing Institute of Pharmacology and Toxicology, 27 Taiping Road, Beijing 100850, PR China. Electronic address: zhyouzhi@aliyun.com.
You-Zhi
YZ
Zhang
Department of New Drug Evaluation, Beijing Institute of Pharmacology and Toxicology, 27 Taiping Road, Beijing 100850, PR China.
Li
L
Yuan
Department of New Drug Evaluation, Beijing Institute of Pharmacology and Toxicology, 27 Taiping Road, Beijing 100850, PR China.
Yun-Feng
YF
Li
Department of New Drug Evaluation, Beijing Institute of Pharmacology and Toxicology, 27 Taiping Road, Beijing 100850, PR China. Electronic address: jinli9802@163.com.
Jin
J
Li
D000367:Age Factors; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects*; D004390:Chlorpyrifos / Q000633:toxicity*; D003863:Depression / Q000139:chemically induced* / Q000523:psychology; D005247:Feeding Behavior / Q000187:drug effects; D006380:Helplessness, Learned; D007306:Insecticides / Q000633:toxicity*; D008297:Male; D017207:Rats, Sprague-Dawley; D013315:Stress, Psychological / Q000139:chemically induced* / Q000523:psychology; D013550:Swimming
D007306:Insecticides; D004390:Chlorpyrifos
Adolescence; Animal model; Children; Chlorpyrifos; Depression; Organophosphate pesticide