Determination of Polyphenol Components of Korean Prostrate Spurge (Euphorbia supina) by Using Liquid Chromatography-Tandem Mass Spectrometry: Overall Contribution to Antioxidant Activity.
The Korean prostrate spurge Euphorbia supina is a weed that has been used in folk medicine in Korea against a variety of diseases. Nine polyphenols were characterized for this plant by using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) and the results were compared with the literature data. The individual components were validated using the calibration curves of structurally related external standards and quantified for the first time by using the validated method. Correlation coefficients (r (2)) were >0.9907. The limit of detection and limit of quantification of the method were >0.028 mg/L and 0.094 mg/L, respectively. Recoveries measured at 50 mg/L and 100 mg/L were 76.1-102.8% and 85.2-98.6%, respectively. The total amount of the identified polyphenols was 3352.9 ± 2.8 mg/kg fresh plant. Quercetin and kaempferol derivatives formed 84.8% of the total polyphenols. The antioxidant activities of the flavonoids were evaluated in terms of 1,1-diphenyl-2-picrylhydrazyl and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation-scavenging activity, and the reducing power showed a dose-dependent increase. Cell viability was effectively suppressed at polyphenol mixture concentrations >250 mg/L.
Department of Chemistry and Research Institute of Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Yi
Y
Song
Department of Chemistry and Research Institute of Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Sung Woo
SW
Jeong
Department of Internal Medicine, Institute of Health Sciences and Gyeongnam Regional Cancer Center, Gyeongsang National University, Jinju 660-702, Republic of Korea.
Won Sup
WS
Lee
Department of Chemistry and Research Institute of Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Semin
S
Park
Department of Chemistry and Research Institute of Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Yun-Hi
YH
Kim
Research Institute of Life Science and College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Gon-Sup
GS
Kim
Department of Food and Nutrition, Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Soo Jung
SJ
Lee
Division of High Technology Materials Research, Busan Center, Korea Basic Science Institute (KBSI), Busan 618-230, Republic of Korea.
Jong Sung
JS
Jin
Department of Dermatology Institute of Health Science, Gyeongsang National University Hospital, Jinju 660-702, Republic of Korea.
Chi-Yeon
CY
Kim
Department of Chemistry and Research Institute of Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Ji Eun
JE
Lee
Department of Chemistry and Research Institute of Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Se Yun
SY
Ok
Department of Chemical Education and Research Institute of Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Ki-Min
KM
Bark
Department of Chemistry and Research Institute of Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Sung Chul
SC
Shin