Long-term exposure to decabrominated diphenyl ether impairs CD8 T-cell function in adult mice.
Polybrominated diphenyl ethers (PBDEs) are ubiquitous environmental pollutants that accumulate to high levels in human populations that are subject to occupational or regional industry exposure. PBDEs have been shown to affect human neuronal, endocrine and reproductive systems, but their effect on the immune system is not well understood. In this study, experimental adult mice were intragastrically administered 2,2',3,3',4,4',5,5',6,6'-decabromodiphenyl ether (BDE-209) at doses of 8, 80 or 800 mg/kg of body weight (bw) at 2-day intervals. Our results showed that continuous exposure to BDE-209 resulted in high levels of BDE-209 in the plasma that approached the levels found in people who work in professions with high risks of PDBE exposure. Reduced leukocytes, decreased cytokine (IFN-γ, IL-2 and TNF-α) production and lower CD8 T-cell proliferation were observed in the mice exposed to BDE-209. Additionally, mice with long-term BDE-209 exposure had lower numbers of antigen-specific CD8 T cells after immunization with recombinant Listeria monocytogenes expressing ovalbumin (rLm-OVA) and the OVA-specific CD8 T cells had reduced functionality. Taken together, our study demonstrates that continuous BDE-209 exposure causes adverse effects on the number and functionality of immune cells in adult mice.
1] Shanghai Institute of Immunology, Institute of Medical Sciences, Shanghai Jiaotong University School of Medicine, Shanghai, China [2] College of Public Health, Shanghai Jiaotong University School of Medicine, Shanghai, China.
Weihong
W
Zeng
Shanghai Institute of Immunology, Institute of Medical Sciences, Shanghai Jiaotong University School of Medicine, Shanghai, China.
Ying
Y
Wang
1] Shanghai Institute of Immunology, Institute of Medical Sciences, Shanghai Jiaotong University School of Medicine, Shanghai, China [2] Department of Dermatology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.
Zhicui
Z
Liu
Shanghai Institute of Immunology, Institute of Medical Sciences, Shanghai Jiaotong University School of Medicine, Shanghai, China.
Asma
A
Khanniche
College of Public Health, Shanghai Jiaotong University School of Medicine, Shanghai, China.
Qingliang
Q
Hu
College of Public Health, Shanghai Jiaotong University School of Medicine, Shanghai, China.
Yan
Y
Feng
Shanghai Institute of Immunology, Institute of Medical Sciences, Shanghai Jiaotong University School of Medicine, Shanghai, China.
Weiyi
W
Ye
Shanghai Institute of Immunology, Institute of Medical Sciences, Shanghai Jiaotong University School of Medicine, Shanghai, China.
Jianglong
J
Yang
Shanghai Institute of Immunology, Institute of Medical Sciences, Shanghai Jiaotong University School of Medicine, Shanghai, China.
Shujun
S
Wang
Shanghai Institute of Immunology, Institute of Medical Sciences, Shanghai Jiaotong University School of Medicine, Shanghai, China.
Lin
L
Zhou
1] Shanghai Institute of Immunology, Institute of Medical Sciences, Shanghai Jiaotong University School of Medicine, Shanghai, China [2] Department of Microbiology, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA.
Hao
H
Shen
College of Public Health, Shanghai Jiaotong University School of Medicine, Shanghai, China.
Yan
Y
Wang
D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000187:drug effects* / Q000276:immunology / Q000382:microbiology; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D015536:Down-Regulation; D004781:Environmental Exposure / Q000009:adverse effects; D005260:Female; D055768:Halogenated Diphenyl Ethers / Q000008:administration & dosage* / Q000009:adverse effects; D006801:Humans; D008088:Listeriosis / Q000235:genetics / Q000276:immunology*; D018655:Lymphocyte Count; D051379:Mice; D008810:Mice, Inbred C57BL; D023421:Models, Animal; D010047:Ovalbumin / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D013997:Time Factors
D000942:Antigens, Bacterial; D016207:Cytokines; D055768:Halogenated Diphenyl Ethers; D010047:Ovalbumin; C010902:decabromobiphenyl ether