[Suppression of abdominal aortic aneurysm by hydrogen through chemokine-like factor1].
OBJECTIVE
To explore the mechanism of hydrogen on the intervention of abdominal aortic aneurysm (AAA).


METHODS
Healthy male Sprague-Dawley rats were divided into AAA group and AAA intervention group (saturated hydrogen saline administered intraperitoneally once daily). AAA was induced by infiltration of abdominal aorta with 0.5 mol/L calcium chloride. Twenty-eight days later, the diameter of the aorta was measured, and the aortic tissue was exercised for histological examination. The protein location and expression of rCklf1 and matrix metalloproteinase 2 (MMP2) in aortic tissue were observed by immunohistochemistry staining. The mRNA expression of rCklf1 and MMP2 underwent real-time PCR.


RESULTS
Dilatation rate of abdominal aorta of AAA group and AAA intervention group was (174 ± 21)% and [(82 ± 23)%, P < 0.01] respectively. The tissue relative mRNA expression of rCklf1 was 5.10 ± 0.33 and 1.34 ± 0.29 (P < 0.01). The relative mRNA expression of MMP2 was 10.28 ± 0.35 and 2.40 ± 0.97 (P < 0.01). The proteins expression of rCklf1 and MMP2 were mainly increased in the damaged elastic fibers of AAA group. Compared with AAA group, the AAA intervention group had less significant positive expression of rCklf1 and MMP2 protein, infiltration of inflammation, destruction and degradation of elastic fibers.


CONCLUSIONS
Hydrogen which may contribute to reduce rCklf1 expression prevents infiltration of inflammation and expression of MMP2 thus decreasing destruction and degradation of elastic fibers, therefore ameliorates development of AAA.
Department of Vascular Surgery,General Hospital of People's Liberation Army, Beijing 100853, China.
Feng
F
Chen
Tao
T
Zhang
Jiang
J
Xiong
Email: pla301dml@vip.sina.com.
Wei
W
Guo
Xiujie
X
Pan
Chenyang
C
Shen
Yuxiang
Y
Song
Senhao
S
Jia
Jie
J
Liu
D000818:Animals; D001012:Aorta, Abdominal / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D017544:Aortic Aneurysm, Abdominal / Q000378:metabolism* / Q000473:pathology; D018925:Chemokines / Q000378:metabolism*; D004195:Disease Models, Animal; D006859:Hydrogen / Q000494:pharmacology*; D062792:MARVEL Domain-Containing Proteins / Q000378:metabolism*; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
D018925:Chemokines; C486623:Cklf protein, rat; D062792:MARVEL Domain-Containing Proteins; D006859:Hydrogen; D020778:Matrix Metalloproteinase 2; C522363:Mmp2 protein, rat