Detection and differentiation of herpes simplex viruses by use of the viper platform: advantages, limitations, and concerns.
The Viper HSV-Q(x) assay was evaluated for the detection of herpes simplex virus 1 (HSV-1) and HSV-2 in specimens from oral, anogenital, and other miscellaneous sites. The HSV-Q(x) assay was found to be highly sensitive and accurate; however, a gray zone may be required for specimens with values falling between 50 and 800 maximum relative fluorescence units.
Dalhousie University, Halifax, Nova Scotia, Canada.
Amanda L S
AL
Lang
Division of Microbiology, Department of Pathology and Laboratory Medicine, Capital District Health Authority (CDHA), Halifax, Nova Scotia, Canada.
Catherine
C
Roberts
Department of Microbiology, Mount Sinai Hospital/University Health Network and University of Toronto, Toronto, Ontario, Canada.
Tony
T
Mazzulli
Division of Microbiology, Department of Pathology and Laboratory Medicine, Capital District Health Authority (CDHA), Halifax, Nova Scotia, Canada Dalhousie University, Halifax, Nova Scotia, Canada.
Todd F
TF
Hatchette
Division of Microbiology, Department of Pathology and Laboratory Medicine, Capital District Health Authority (CDHA), Halifax, Nova Scotia, Canada Dalhousie University, Halifax, Nova Scotia, Canada jason.leblanc@cdha.nshealth.ca.
Jason J
JJ
LeBlanc
D005453:Fluorescence; D005835:Genitalia / Q000821:virology; D006558:Herpes Genitalis / Q000175:diagnosis* / Q000821:virology*; D006561:Herpes Simplex / Q000175:diagnosis* / Q000821:virology*; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods* / Q000592:standards; D009055:Mouth / Q000821:virology; D012680:Sensitivity and Specificity; D018139:Simplexvirus / Q000145:classification* / Q000302:isolation & purification*