GradientOptimizer: an open-source graphical environment for calculating optimized gradients in reversed-phase liquid chromatography.
We here present GradientOptimizer, an intuitive, lightweight graphical user interface to design nonlinear gradients for separation of peptides by reversed-phase liquid chromatography. The software allows to calculate three types of nonlinear gradients, each of them optimizing a certain retention time distribution of interest. GradientOptimizer is straightforward to use, requires minimum processing of the input files, and is supported under Windows, Linux, and OS X platforms. The software is open-source and can be downloaded under an Apache 2.0 license at https://github.com/statisticalbiotechnology/NonlinearGradientsUI.
Science for Life Laboratory, Department of Biochemistry and Biophysics, Stockholm University, Solna, Sweden.
Luminita
L
Moruz
Lukas
L
Käll
D000465:Algorithms; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D019295:Computational Biology; D003196:Computer Graphics; D006801:Humans; D012984:Software; D014584:User-Computer Interface
Bioinformatics; Chromatography; RPLC; Retention time