A mesoscale connectome of the mouse brain.
Comprehensive knowledge of the brain's wiring diagram is fundamental for understanding how the nervous system processes information at both local and global scales. However, with the singular exception of the C. elegans microscale connectome, there are no complete connectivity data sets in other species. Here we report a brain-wide, cellular-level, mesoscale connectome for the mouse. The Allen Mouse Brain Connectivity Atlas uses enhanced green fluorescent protein (EGFP)-expressing adeno-associated viral vectors to trace axonal projections from defined regions and cell types, and high-throughput serial two-photon tomography to image the EGFP-labelled axons throughout the brain. This systematic and standardized approach allows spatial registration of individual experiments into a common three dimensional (3D) reference space, resulting in a whole-brain connectivity matrix. A computational model yields insights into connectional strength distribution, symmetry and other network properties. Virtual tractography illustrates 3D topography among interconnected regions. Cortico-thalamic pathway analysis demonstrates segregation and integration of parallel pathways. The Allen Mouse Brain Connectivity Atlas is a freely available, foundational resource for structural and functional investigations into the neural circuits that support behavioural and cognitive processes in health and disease.
1] Allen Institute for Brain Science, Seattle, Washington 98103, USA [2].
Seung Wook
SW
Oh
1] Allen Institute for Brain Science, Seattle, Washington 98103, USA [2].
Julie A
JA
Harris
1] Allen Institute for Brain Science, Seattle, Washington 98103, USA [2].
Lydia
L
Ng
Allen Institute for Brain Science, Seattle, Washington 98103, USA.
Brent
B
Winslow
Allen Institute for Brain Science, Seattle, Washington 98103, USA.
Nicholas
N
Cain
Allen Institute for Brain Science, Seattle, Washington 98103, USA.
Stefan
S
Mihalas
Allen Institute for Brain Science, Seattle, Washington 98103, USA.
Quanxin
Q
Wang
Allen Institute for Brain Science, Seattle, Washington 98103, USA.
Chris
C
Lau
Allen Institute for Brain Science, Seattle, Washington 98103, USA.
Leonard
L
Kuan
Allen Institute for Brain Science, Seattle, Washington 98103, USA.
Alex M
AM
Henry
Allen Institute for Brain Science, Seattle, Washington 98103, USA.
Marty T
MT
Mortrud
Allen Institute for Brain Science, Seattle, Washington 98103, USA.
Benjamin
B
Ouellette
Allen Institute for Brain Science, Seattle, Washington 98103, USA.
Thuc Nghi
TN
Nguyen
Allen Institute for Brain Science, Seattle, Washington 98103, USA.
Staci A
SA
Sorensen
Allen Institute for Brain Science, Seattle, Washington 98103, USA.
Clifford R
CR
Slaughterbeck
Allen Institute for Brain Science, Seattle, Washington 98103, USA.
Wayne
W
Wakeman
Allen Institute for Brain Science, Seattle, Washington 98103, USA.
Yang
Y
Li
Allen Institute for Brain Science, Seattle, Washington 98103, USA.
David
D
Feng
Allen Institute for Brain Science, Seattle, Washington 98103, USA.
Anh
A
Ho
Allen Institute for Brain Science, Seattle, Washington 98103, USA.
Eric
E
Nicholas
Allen Institute for Brain Science, Seattle, Washington 98103, USA.
Karla E
KE
Hirokawa
Allen Institute for Brain Science, Seattle, Washington 98103, USA.
Phillip
P
Bohn
Allen Institute for Brain Science, Seattle, Washington 98103, USA.
Kevin M
KM
Joines
Allen Institute for Brain Science, Seattle, Washington 98103, USA.
Hanchuan
H
Peng
Allen Institute for Brain Science, Seattle, Washington 98103, USA.
Michael J
MJ
Hawrylycz
Allen Institute for Brain Science, Seattle, Washington 98103, USA.
John W
JW
Phillips
Allen Institute for Brain Science, Seattle, Washington 98103, USA.
John G
JG
Hohmann
Allen Institute for Brain Science, Seattle, Washington 98103, USA.
Paul
P
Wohnoutka
Laboratory of Systems Neuroscience, National Institute of Mental Health, Bethesda, Maryland 20892, USA.
Charles R
CR
Gerfen
Allen Institute for Brain Science, Seattle, Washington 98103, USA.
Christof
C
Koch
Allen Institute for Brain Science, Seattle, Washington 98103, USA.
Amy
A
Bernard
Allen Institute for Brain Science, Seattle, Washington 98103, USA.
Chinh
C
Dang
Allen Institute for Brain Science, Seattle, Washington 98103, USA.
Allan R
AR
Jones
Allen Institute for Brain Science, Seattle, Washington 98103, USA.
Hongkui
H
Zeng
D000818:Animals; D001271:Atlases as Topic; D001369:Axons / Q000502:physiology; D001921:Brain / Q000033:anatomy & histology* / Q000166:cytology*; D002540:Cerebral Cortex / Q000166:cytology; D063132:Connectome; D003342:Corpus Striatum / Q000166:cytology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008959:Models, Neurological; D056347:Neuroanatomical Tract-Tracing Techniques; D013788:Thalamus / Q000166:cytology