Molecularly imprinted polymers with novel functional monomer for selective solid-phase extraction of gastrodin from the aqueous extract of Gastrodia elata.
Molecular imprinted polymers (MIPs) with high selectivity and affinity to gastrodin in water were designed using allyl 2,3,4,6-tetra-O-acetyl-glucopyranoside (TAGL) and 1,2,3,4,5-pentafluoro-6-vinylbenzene (PFVB) as novel functional monomers. Binding characterization of pre-polymerization complexes was researched by nuclear magnetic resonance (NMR) and the MIPs were characterized by scanning electron microscopy (SEM). The properties involving adsorption isotherm, adsorption kinetics and selective recognition capacity were evaluated. The MIPs/TAGL exhibited good site accessibility in which it only took 30min to achieve adsorption equilibrium and highly selective recognition for the template. Furthermore, the performance of the MIPs/TAGL as solid phase extraction material was investigated in detail and hot water at 50°C served as the eluting solvent. Pure gastrodin with the recovery of 76.6% was obtained from the aqueous extract of Gastrodia elata roots.
Shandong Analysis and Test Center, Shandong Academy of Sciences, 19 Keyuan Street, Jinan, 250014, China.
Wenhua
W
Ji
Shandong Analysis and Test Center, Shandong Academy of Sciences, 19 Keyuan Street, Jinan, 250014, China; College of Food Science and Engineering, Shandong Agricultural University, Taian, China.
Lingxiao
L
Chen
Shandong Analysis and Test Center, Shandong Academy of Sciences, 19 Keyuan Street, Jinan, 250014, China; College of Food Science and Engineering, Shandong Agricultural University, Taian, China.
Xiuli
X
Ma
Shandong Analysis and Test Center, Shandong Academy of Sciences, 19 Keyuan Street, Jinan, 250014, China; National Resource Center for Chinese Materia Medica, State Key Laboratory Breeding Base of Dao-di Herbs, China Academy of Chinese Medical Sciences, Beijing, 100700, China. Electronic address: wangx@sdas.org.
Xiao
X
Wang
Shandong Analysis and Test Center, Shandong Academy of Sciences, 19 Keyuan Street, Jinan, 250014, China.
Qianshan
Q
Gao
Shandong Analysis and Test Center, Shandong Academy of Sciences, 19 Keyuan Street, Jinan, 250014, China.
Yanling
Y
Geng
National Resource Center for Chinese Materia Medica, State Key Laboratory Breeding Base of Dao-di Herbs, China Academy of Chinese Medical Sciences, Beijing, 100700, China. Electronic address: luqihuang@126.com.
Luqi
L
Huang
D000498:Allyl Compounds / Q000737:chemistry*; D001592:Benzyl Alcohols / Q000302:isolation & purification*; D031668:Gastrodia / Q000737:chemistry*; D005960:Glucosides / Q000737:chemistry* / Q000302:isolation & purification; D006860:Hydrogen Bonding; D054802:Molecular Imprinting; D010936:Plant Extracts / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry; D058105:Polymerization; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry; D052616:Solid Phase Extraction; D012997:Solvents; D013343:Styrenes / Q000737:chemistry*; D014867:Water
D000498:Allyl Compounds; D001592:Benzyl Alcohols; D005960:Glucosides; D010936:Plant Extracts; D011108:Polymers; D012997:Solvents; D013343:Styrenes; D014867:Water; C045345:gastrodin
Gastrodin; Molecular imprinted polymers; Novel functional monomer; Solid-phase extraction