In vivo performance of a novel fluorinated magnetic resonance imaging agent for functional analysis of bile acid transport.
A novel trifluorinated cholic acid derivative, CA-lys-TFA, was designed and synthesized for use as a tool to measure bile acid transport noninvasively using magnetic resonance imaging (MRI). In the present study, the in vivo performance of CA-lys-TFA for measuring bile acid transport by MRI was investigated in mice. Gallbladder CA-lys-TFA content was quantified using MRI and liquid chromatography/tandem mass spectrometry. Results in wild-type (WT) C57BL/6J mice were compared to those in mice lacking expression of Asbt, the ileal bile acid transporter. (19)F signals emanating from the gallbladders of WT mice 7 h after oral gavage with 150 mg/kg CA-lys-TFA were reproducibly detected by MRI. Asbt-deficient mice administered the same dose had undetectable (19)F signals by MRI, and gallbladder bile CA-lys-TFA levels were 30-fold lower compared to WT animals. To our knowledge, this represents the first report of in vivo imaging of an orally absorbed drug using (19)F MRI. Fluorinated bile acid analogues have potential as tools to measure and detect abnormal bile acid transport by MRI.
Department of Pharmaceutical Sciences, University of Maryland School of Pharmacy , Baltimore, Maryland 21230, United States.
Diana
D
Vivian
Kunrong
K
Cheng
Sandeep
S
Khurana
Su
S
Xu
Edwin H
EH
Kriel
Paul A
PA
Dawson
Jean-Pierre
JP
Raufman
James E
JE
Polli
D000818:Animals; D001647:Bile Acids and Salts / Q000378:metabolism*; D001692:Biological Transport / Q000502:physiology; D002853:Chromatography, Liquid; D005704:Gallbladder / Q000378:metabolism; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D053719:Tandem Mass Spectrometry
D001647:Bile Acids and Salts