Antiinflammatory Activity of Gynura bicolor ( Hóng Fèng Cài) Ether Extract Through Inhibits Nuclear Factor Kappa B Activation.
This study investigated effects of the Gynura bicolor (Roxb. and Willd.) DC. ether extract (GBEE) on nitric oxide (NO) and prostaglandin (PG)E2 production on the lipopolysaccharide (LPS)-induced inflammatory response in RAW 264.7 cells. A composition analysis of GBEE showed that the major compounds were b-carotene, chlorophyll, and quercetin, respectively. Furthermore, NO and PGE2 levels of 120 μg/ml GBEE-treated cells were 70% and 9.8%, respectively, than those of cells treated with LPS alone. Immunoblots assays showed that the GBEE dose-dependently suppressed LPS-induced inducible NO synthase (iNOS) and cyclooxygenase (COX)-2 protein levels. The GBEE significantly decreased cytosolic phosphorylated (p)-IκBa and nuclear p65 protein expressions. Electrophoresis mobility shift assays indicated that the GBEE effectively inhibited nuclear factor kappa B (NF-κB) activation induced by LPS. These results support a role of the GBEE in suppressing activation of NF-κB to inhibit NO and PGE2 production in the LPS-induced inflammatory response by RAW 264.7 cells.
Department of Nutrition and Health Sciences, Chang Jung Christian University, Tainan 711, Taiwan.
Chih-Chung
CC
Wu
Department of Nutrition, China Medical University, Taichung 404, Taiwan.
Chong-Kuei
CK
Lii
Department of Nutrition, Chung Shan Medical University, Taichung 402, Taiwan.
Kai-Li
KL
Liu
Department of Nutrition, Chung Shan Medical University, Taichung 402, Taiwan.
Pei-Yin
PY
Chen
Department of Seafood Sciences, National Kaohsiung Marine University, Kaohsiung 811, Taiwan.
Shu-Ling
SL
Hsieh
Cells; Cyclooxygenase; Gynura bicolor; Inducible nitric oxide synthase; Nuclear transcription factor-κB