Transcriptional changes in organoculture of full-thickness human skin following topical application of all-trans retinoic acid.
In this study, we developed an organoculture of human skin to investigate the effect of topical applied all-trans retinoic acid using a gene array approach. We could by using this approach confirm previous studies on genes activated by RA in keratinocyte monocultures and also provide new insights on genes that are relevant to RA-activation in human skin. The results in the present study show this model represent a valuable pre-clinical model for studying the effects of retinoids in skin.
Oriflame Skin Research Institute, Mäster Samuelsgatan 56, Stockholm, 11121, Sweden.
J M
JM
Gillbro
T
T
Al-Bader
M
M
Westman
M J
MJ
Olsson
A
A
Mavon
D000328:Adult; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D012313:RNA / Q000737:chemistry / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D012867:Skin / Q000187:drug effects* / Q000378:metabolism*; D012879:Skin Physiological Phenomena / Q000187:drug effects*; D014158:Transcription, Genetic / Q000187:drug effects; D014212:Tretinoin / Q000008:administration & dosage / Q000494:pharmacology*; D055815:Young Adult
D014212:Tretinoin; D012313:RNA
acne; cell culture; dandruff; elisa; genomics; proteomics; rosacea; skin culture; striae skin physiology; structure