RuSi@Ru(bpy)3(2+)/Au@Ag2S nanoparticles electrochemiluminescence resonance energy transfer system for sensitive DNA detection.
This work describes a new electrochemiluminescence resonance energy transfer (ECL-RET) system with graphene oxide(GO)-Au/RuSi@Ru(bpy)3(2+)/chitosan (CS) composites as the ECL donor and Au@Ag2S nanoparticles (NPs) as ECL the acceptor for the first time. The ECL signal observed by the application of GO-Au/RuSi@Ru(bpy)3(2+)/CS composites was enhanced for 5-fold compared to that of RuSi@Ru(bpy)3(2+)/CS in the presence of coreactant tripropylamine (TPA) due to the increased surface area and improved electrical conductivity by using graphene oxide-gold nanoparticles (GO-Au) composite materials. In addition, we synthesized Au@Ag2S core-shell NPs, whose UV-vis absorption spectrum shows good spectral overlap with the ECL spectrum of GO-Au/RuSi@Ru(bpy)3(2+)/CS composites by adjusting the amount of Na2S and AgNO3 in the process of synthesis. The distance between energy donor and acceptor was studied to get the highly effective ECL-RET. Then, this ECL-RET system was developed for sensitive and specific detection of target DNA, and the ECL quenching efficiency (ΔI/I0, ΔI = I0 - I) was found to be logarithmically related to the concentration of the target DNA in the range from 10 aM to 10 pM.
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing 210093, China.
Mei-Sheng
MS
Wu
Li-Jing
LJ
He
Jing-Juan
JJ
Xu
Hong-Yuan
HY
Chen
D001483:Base Sequence; D004247:DNA / Q000032:analysis*; D015342:DNA Probes; D055664:Electrochemical Techniques; D004735:Energy Transfer; D006046:Gold / Q000737:chemistry; D049449:Luminescence; D053768:Metal Nanoparticles; D046529:Microscopy, Electron, Transmission; D012428:Ruthenium / Q000737:chemistry; D012834:Silver / Q000737:chemistry
D015342:DNA Probes; D012834:Silver; D006046:Gold; D012428:Ruthenium; D004247:DNA