Immunohistochemical and electron microscopy aspects of the nerve structures from the dental pulp.
In this study, we have done an immunohistochemical and an electron microscopy examination of normal and inflamed human dental pulp specimens in order to evaluate the morphological aspects of the nerve structures from the dental pulp. The S100 protein immunohistochemical marking allowed us to observe the trajectory of the pulp nervous structures, which appear as continuous bands of high intensity at radicular level, coronary branch out and some branches cross the odontoblastic layer and penetrate in predentin along the dentinal tubules. It appears that not only the nerve structures are positive S100 protein but also macrophages or dendritic cells. The electron microscopic part presents the ultrastructure details of the nervous structures observed on the samples from normal and inflamed pulp conjunctive tissues. Even in acute pulpitis no ultrastructural changes occur in the nerve fibers, prolonged exposure to noxious factors may lead to changes like nerve sprouting.
Department of Dental Materials, Faculty of Dental Medicine, University of Medicine and Pharmacy of Craiova, Romania; manoleahoria@gmail.com.
Horia
H
Manolea
Nicolae
N
Vasile
Mirela
M
Opri
Adrian
A
Fronie
Mihai Raul
MR
Popescu
D003782:Dental Pulp / Q000294:innervation*; D006801:Humans; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D009417:Nerve Tissue / Q000166:cytology* / Q000648:ultrastructure*; D011901:Ranvier's Nodes / Q000648:ultrastructure; D009418:S100 Proteins / Q000378:metabolism; D012583:Schwann Cells / Q000166:cytology / Q000648:ultrastructure
D009418:S100 Proteins