Protective effect of Aronia melanocarpa fruit juice in a model of cisplatin-induced cytotoxicity in vitro.
The aim of the present study was to investigate the protective potential of Aronia melanocarpa fruit juice in a model of cisplatin-induced cytotoxicity in the human embryonal kidney cell line HEK293T.



The cellular viability was assessed using the MTT-dye reduction assay based on the reduction of the yellow tetrazolium dye MTT to a violet formazan product via the mitochondrial succinate dehydrogenase in viable cells. Cisplatin was applied in various concentrations either alone or after a 24-hour pretreatment of the cells with Aronia melanocarpa fruit juice at 0.1 and 0.05 mg/ml. The half maximal inhibitory concentrations (IC50 values) were derived from the concentration-response curves to cisplatin.



Applied alone, the anticancer drug caused a prominent decrease of cellular viability with IC50 8.3 +/- 1.1 microM. The juice proved to significantly ameliorate the in vitro cytotoxicity of the platinum drug, in a concentration-dependent manner. The pretreatment of the cells with Aronia melanocarpa fruit juice resulted in a significant increase (p < 0.001) of IC50 for cisplatin to 25.1 +/- 2.7 microM (at 0.05 mg/ml) and 34.4 +/- 3.4 microM (at 0.1 mg/ml), respectively.



The protective effect of Aronia melanocarpa fruit juice observed in this study is most probably due to its well appreciated antioxidant activity as oxidative stress plays a central role in the toxic effects of cisplatin.
Department of Preclinical and Clinical Pharmacology, Medical University Prof. Dr. Paraskev Stoyanov, Varna, Bulgaria
Stefka V
SV
Valcheva-Kuzmanova
Department of Preclinical and Clinical Pharmacology, Medical University Prof. Dr. Paraskev Stoyanov, Varna, Bulgaria
Anna B
AB
Beronova
Department of Pharmacology, Pharmacotherapy and Toxicology, Faculty of Pharmacy, Medical University of Sofia, Bulgaria
Georgi Tz
GT
Momekov
D000970:Antineoplastic Agents / Q000633:toxicity*; D001628:Beverages; D002945:Cisplatin / Q000633:toxicity*; D019610:Cytoprotection; D057809:HEK293 Cells; D006801:Humans; D020128:Inhibitory Concentration 50; D007668:Kidney / Q000187:drug effects*; D046936:Photinia
D000970:Antineoplastic Agents; D002945:Cisplatin