Influence of different iodinated contrast media on the induction of DNA double-strand breaks after in vitro X-ray irradiation.
The objective of this work was to examine differences in DNA double-strand break induction in peripheral blood lymphocytes after in vitro X-ray irradiation between iodinated contrast agents. Four different iodinated X-ray contrast agents--three of them with two different iodine concentrations--and mannitol (negative control; concentration of 150 mg mannitol per ml blood) were pipetted into blood samples so that there was a concentration of 0, 7.5 or 15 mg of iodine per ml blood in the samples. Negative controls without contrast medium (0 mg of iodine per ml blood) were also processed for every irradiation dose. The tubes were exposed to 0, 20 or 500 mGy in vitro X-ray irradiation. After that, the lymphocytes were separated by using density-gradient centrifugation. Fluorescence microscopy was applied to determine the average number of γH2AX-foci per lymphocyte in the presence or absence of different contrast media or mannitol. Differences in the number of γH2AX-foci were statistically analysed by one-way ANOVA and post-hoc Tukey's honestly significant difference test. Iodinated contrast agents led to a statistically significant increase in DNA double-strand breaks after in vitro irradiation. This effect increased statistically significant with rising radiation dose and appeared independent of the contrast agent used (iopromid, iodixanol, iomeprol, iopamidol). A statistically significant difference in DNA damage between the different tested contrast agents was not found. Therefore, the increase in DNA double-strand breaks depends solely on the amount of iodine applied. For evaluation of clinical consequences, our findings could be tested in further animal studies.
Department of Diagnostic and Interventional Radiology, Philipps University, Marburg, Germany.
Christoph K W
CK
Deinzer
Daniela
D
Danova
Beate
B
Kleb
Klaus J
KJ
Klose
Johannes T
JT
Heverhagen
D000328:Adult; D003287:Contrast Media / Q000009:adverse effects*; D053903:DNA Breaks, Double-Stranded / Q000187:drug effects / Q000528:radiation effects; D004249:DNA Damage / Q000187:drug effects / Q000528:radiation effects; D006657:Histones / Q000378:metabolism; D006801:Humans; D007455:Iodine / Q000008:administration & dosage* / Q000009:adverse effects / Q000097:blood; D008214:Lymphocytes / Q000187:drug effects* / Q000528:radiation effects; D008297:Male; D008353:Mannitol / Q000008:administration & dosage / Q000097:blood; D008856:Microscopy, Fluorescence; D014057:Tomography, X-Ray Computed; D014965:X-Rays
D003287:Contrast Media; C468783:H2AX protein, human; D006657:Histones; D008353:Mannitol; D007455:Iodine
DNA double-strand breaks; experimental studies; fluorescence microscopy; iodinated contrast media; radiation damage; γH2AX-foci