Poly (hexamethylene biguanide) adsorption on hydrogen peroxide treated Ti-Al-V alloys and effects on wettability, antimicrobial efficacy, and cytotoxicity.
An effective amount of the antiseptic agent PHMB cannot simply be placed on the surface of titanium alloys where hydrocarbons were removed by different purification procedures. Pre-treatment of Ti6Al4V specimen with 5% H2O2 in 24 h results in extra introduced -OH and -COOH groups as well as an adsorbed water film on the surface, which provide the base for the subsequent formation of a relatively stable and multi-layered PHMB film. The superficially adhering PHMB film produces no adverse effects on MG63 cells within a 48 h-cell culture, but promotes the initial attachment and spreading of the osteoblasts on the modified Ti6Al4V surface within 15 min. After direct bacterial inoculation of the active sample, the PHMB film reacts antimicrobially against Gram-positive (Staphylococcus aureus, Staphylococcus epidermidis) and Gram-negative strains (Pseudomonas aeruginosa, Escherichia coli) after surface contact. The bactericidal efficacy is only slightly reduced after using of the same specimen for re-testing the antibacterial activity. MG63 cells adhere and proliferate within 48 h on a PHMB film-containing Ti6Al4V surface, which has been pre-contaminated with S. aureus. Bacterial biofilms were only revealed in controls without PHMB.
Institute of Hygiene and Environmental Medicine, Walther-Rathenau-Str. 49a, University Medicine, D-17475 Greifswald, Germany.
Gerald
G
Müller
Institute of Hygiene and Environmental Medicine, Walther-Rathenau-Str. 49a, University Medicine, D-17475 Greifswald, Germany.
Hicham
H
Benkhai
Institute of Hygiene and Environmental Medicine, Walther-Rathenau-Str. 49a, University Medicine, D-17475 Greifswald, Germany.
Rutger
R
Matthes
Leibniz-Institute for Plasma Science and Technology (INP e.V.), Felix-Hausdorff-Str. 2, D-17489 Greifswald, Germany.
Birgit
B
Finke
Institute of Biochemistry, Department of Biophysical Chemistry, Felix-Hausdorff-Str. 4, D-17487 Greifswald, Germany.
Wenke
W
Friedrichs
Institute of Biochemistry, Department of Biophysical Chemistry, Felix-Hausdorff-Str. 4, D-17487 Greifswald, Germany.
Norman
N
Geist
Institute of Biochemistry, Department of Biophysical Chemistry, Felix-Hausdorff-Str. 4, D-17487 Greifswald, Germany.
Walter
W
Langel
Institute of Hygiene and Environmental Medicine, Walther-Rathenau-Str. 49a, University Medicine, D-17475 Greifswald, Germany. Electronic address: Kramer@uni-greifswald.de.
Axel
A
Kramer
D000327:Adsorption; D000497:Alloys; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D000891:Anti-Infective Agents, Local / Q000737:chemistry / Q000494:pharmacology; D001645:Biguanides / Q000737:chemistry / Q000494:pharmacology*; D018441:Biofilms; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry*; D010006:Osteoblasts / Q000187:drug effects / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects; D013212:Staphylococcus epidermidis / Q000187:drug effects; D014025:Titanium / Q000737:chemistry*; D017155:Wettability / Q000187:drug effects*
D000497:Alloys; D000900:Anti-Bacterial Agents; D000891:Anti-Infective Agents, Local; D001645:Biguanides; C031462:titanium alloy (TiAl6V4); C031233:polihexanide; D006861:Hydrogen Peroxide; D014025:Titanium
Antimicrobial; Biocompatibility; Hydrogen peroxide; In vitro tests; PHMB; Titanium-aluminum-vanadium alloy