Characteristic hydrolyzing of megalosaccharide by human salivary α-amylase and small intestinal enzymes, and its bioavailability in healthy subjects.
The digestibility of Megalosaccharide® (newly developed carbohydrate comprising α-1,4-glucosaccharide) was investigated in vitro and in vivo. Isomaltosyl-megalosaccharide® (IMS) and nigerosyl-megalosaccharide® (NMS) contain 20% and 50% of the megalosaccharide fraction (degree of polymerization (DP) 10-35), respectively. IMS was hydrolyzed readily by α-amylase to oligosaccharides (DP ≤ 7), and a small amount of glucose was produced from oligosaccharides by small intestinal enzymes (SIEs). NMS was partially hydrolyzed by α-amylase to oligosaccharides, and a small amount of glucose produced by SIEs. When IMS and NMS were treated by SIEs after treatment with human saliva α-amylase for a few minutes, IMS and NMS were hydrolyzed readily to glucose. Plasma levels of glucose and insulin upon ingestion of 50 g of IMS or NMS were elevated the same as those for 50 g of glucose, and breath hydrogen was not excreted. These results suggest that IMS and NMS are digestible carbohydrates.
Graduate School of Human Health Science, University of Nagasaki Siebold , Nagayo, Nagasaki , Japan.
Sadako
S
Nakamura
Masayuki
M
Takami
Kenichi
K
Tanabe
Tsuneyuki
T
Oku
D000328:Adult; D001682:Biological Availability; D001786:Blood Glucose / Q000378:metabolism; D004040:Dietary Carbohydrates / Q000378:metabolism*; D004063:Digestion; D005260:Female; D064368:Healthy Volunteers; D006801:Humans; D007421:Intestine, Small / Q000201:enzymology*; D008297:Male; D011134:Polysaccharides / Q000378:metabolism* / Q000493:pharmacokinetics; D055291:Salivary alpha-Amylases / Q000378:metabolism*; D055815:Young Adult
D001786:Blood Glucose; D004040:Dietary Carbohydrates; D011134:Polysaccharides; D055291:Salivary alpha-Amylases
Bioavailability; characteristic hydrolyzing; megalosaccharide; salivary α-amylase; small intestinal enzymes