Atomic force microscopy-coupled microcoils for cellular-scale nuclear magnetic resonance spectroscopy.
We present the coupling of atomic force microscopy (AFM) and nuclear magnetic resonance (NMR) technologies to enable topographical, mechanical, and chemical profiling of biological samples. Here, we fabricate and perform proof-of-concept testing of radiofrequency planar microcoils on commercial AFM cantilevers. The sensitive region of the coil was estimated to cover an approximate volume of 19.4 × 103μm3(19.4 pl). Functionality of the spectroscopic module of the prototype device is illustrated through the detection of1Η resonance in deionized water. The acquired spectra depict combined NMR capability with AFM that may ultimately enable biophysical and biochemical studies at the single cell level.
Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana 47907, USA.
Charilaos
C
Mousoulis
Department of Electrical and Computer Engineering, Purdue University, West Lafayette, Indiana 47907, USA.
Teimour
T
Maleki
Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana 47907, USA ; Department of Electrical and Computer Engineering, Purdue University, West Lafayette, Indiana 47907, USA ; Birck Nanotechnology Center, Purdue University, West Lafayette, Indiana 47907.
Babak
B
Ziaie
Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana 47907, USA.
Corey P
CP
Neu