Unraveling autoimmunity with the adoptive transfer of T cells from TCR-transgenic mice.
Transgenesis of rearranged α and β chains from the T-cell receptor has allowed the generation of a variety of mice with a predetermined T-cell repertoire. These mice have been extensively used as tools to circumvent the low precursor frequency of naturally occurring endogenous T cells. As such, they have been valuable to study pathways of T-cell development in the thymus. In addition, these mice can also be considered as a valuable source of naive and/or memory T cells with a defined specificity. I will comment in this chapter the use of this source of T cells with known antigen reactivity to study in vivo T-cell behavior in the periphery, including during autoimmune responses.
Team 8, Institut Albert Bonniot, 38700, La Tronche, France, Bertrand.Hard@unige.ch.
Huard
H
Bertrand
D019264:Adoptive Transfer; D000818:Animals; D015551:Autoimmunity; D049109:Cell Proliferation; D016207:Cytokines / Q000032:analysis / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D011948:Receptors, Antigen, T-Cell / Q000235:genetics* / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D019076:Transgenes
D016207:Cytokines; D011948:Receptors, Antigen, T-Cell