The abnormal expressions of tristetraprolin and the VEGF family in uraemic rats with peritoneal dialysis.
The effect of peritoneal dialysis (PD) with high-glucose dialysis fluid on the VEGF family, tristetraprolin (TTP), angiogenesis and lymphangiogenesis was investigated. Forty male SD rats were randomised into five groups: normal group, sham operation group, uraemia group, PD 2-week group and PD4-week group. After 4 weeks of PD, microvessel density (MVD) and lymphatic vessel density (LVD) were measured. The expressions of both the VEGF family and TTP were detected. Compared with the normal group, the mRNA expression levels of the VEGF family were significantly increased in the uraemia group (P < 0.05), and also in the PD 2-week group and PD4-week group (P < 0.05) compared with uraemia group. The mRNAs of VEGF-A and VEGF-C in 4-week PD group likewise were significantly increased compared with the 2-week PD group. However, the mRNA expression of TTP was significantly decreased in the uraemia group compared with the normal group (P < 0.05), and also in the PD group compared with the uraemia group (P < 0.05). Compared with the normal group, the protein expressions of TTP were significantly decreased in the uraemia group (P < 0.05), and also in the PD group compared with the uraemia group (P < 0.05). Compared with the normal group, the MVD and LVD counts were gradually increased in the PD group, which was associated with PD time. In addition, the expression of TTP gradually decreased over PD time. High-glucose PD fluid and uraemic circumstance resulted in the abnormal expression of TTP and the VEGF family in a PD time-dependent manner; this may lead to UFF through angiogenesis and lymphangiogenesis.
Department of Nephrology, The First Affiliated Hospital of Zhengzhou University, No.1 Eastern Jianshe Road, Zhengzhou, 450052, Henan, China.
Jing
J
Xiao
Huanhuan
H
Gao
Yunfeng
Y
Jin
Zhihong
Z
Zhao
Jia
J
Guo
Zhangsuo
Z
Liu
Zhanzheng
Z
Zhao
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D008297:Male; D010530:Peritoneal Dialysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D051816:Tristetraprolin / Q000235:genetics / Q000378:metabolism*; D014511:Uremia / Q000378:metabolism* / Q000628:therapy; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism*
D017931:DNA Primers; D051816:Tristetraprolin; D042461:Vascular Endothelial Growth Factor A