Sertraline increases the survival of retinoic acid induced neuronal cells but not glial cells from human mesenchymal stem cells.
An increase in the number of viable in vitro differentiated neuronal cells is important for their use in clinics. A proportion of differentiated cells lose their viability before being used, and therefore we decided to use a pharmacological agent, sertraline, to increase neural cell differentiation and their survival. Purified endometrial stem cells (EnSCs) were examined for neuronal and glial cell specific markers after retinoic acid (RA) and sertraline treatment via RT-PCR, immunocytochemistry and Western blot analysis. The survival of differentiated cells was measured by MTT assay and the frequency of apoptosis, demonstrated by caspase-3-like activity. EnSCs were differentiated into neuronal cells after RA induction. Sertraline increased neuronal cell differentiation by 1.2-fold and their survival by 1.4-fold, and decreased from glial cell differentiation significantly. The findings indicate that sertraline could be used to improve the in vitro differentiation process of stem cells into neuronal cells, and may be involved in regenerative pharmacology in future.
Applied Cell Sciences Department, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran; Stem cell and Tissue Engineering Department, Research Center for Science and Technology in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Javad
J
Verdi
Shiva
S
Sharif
Hamid Reza
HR
Banafshe
Alireza
A
Shoae-Hassani
D000943:Antigens, Differentiation / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects; D002454:Cell Differentiation; D002470:Cell Survival / Q000187:drug effects*; D002478:Cells, Cultured; D004717:Endometrium / Q000166:cytology; D005260:Female; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D009457:Neuroglia / Q000187:drug effects / Q000502:physiology*; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D018696:Neuroprotective Agents / Q000494:pharmacology; D020280:Sertraline / Q000494:pharmacology*; D014212:Tretinoin / Q000494:pharmacology*
D000943:Antigens, Differentiation; D018696:Neuroprotective Agents; D014212:Tretinoin; D020280:Sertraline
cell therapy; differentiation; endometrial stem cell; regenerative pharmacology