Characterization of interleukin-33 and matrix metalloproteinase-28 in serum and their association with disease severity in patients with coronary heart disease.
OBJECTIVE
To investigate serum levels of interleukin (IL)-33 and matrix metalloproteinase-28 (MMP-28) in patients with coronary heart disease (CHD) and to evaluate their association with disease severity.


METHODS
A total of 103 patients with CHD, including 27 cases of acute myocardial infarction (AMI), 33 cases of unstable angina pectoris (UAP) and 43 cases of stable angina pectoris were enrolled to detect serum levels of IL-33 and MMP-28 by enzyme-linked immunosorbent assays. Forty volunteers without CHD served as the control group.


RESULTS
Compared with stable angina pectoris and control groups, serum levels of IL-33 were significantly lower (P<0.01) and serum concentrations of MMP-28 were higher (P<0.05) in AMI and UAP groups. Serum levels of IL-33 in single-vessel, double-vessel and triple-vessel lesion groups were lower than that in the control group (P<0.05), and the differences among the three groups were not significant (P>0.05), whereas only levels of MMP-28 in double-vessel and triple-vessel lesion groups were higher than in the control group (P<0.05). Spearman's correlation analyses showed a negative correlation between serum levels of IL-33 and MMP-28 in AMI and UAP groups (r=-0.596, P<0.05 and r=-0.750, P<0.01). A binary logistic regression analysis showed that IL-33, low-density lipoprotein cholesterol, and MMP-28 may be independent predictors of the occurrence of acute coronary syndrome.


CONCLUSIONS
A decreased level of IL-33 and an elevated concentration of MMP-28 were found in CHD patients and correlated with disease severity. IL-33 and MMP-28 may play important roles in the development of CHD or as markers of disease severity.
aDepartment of Cardiology bInstitute of Clinical Medicine, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, People's Republic of China.
Cong-Lin
CL
Liu
De-Liang
DL
Shen
Kui
K
Zhu
Jun-Nan
JN
Tang
Xiao-Fang
XF
Wang
Li
L
Zhang
Jin-Ying
JY
Zhang
D000328:Adult; D000368:Aged; D060050:Angina, Stable / Q000097:blood* / Q000175:diagnosis / Q000201:enzymology / Q000276:immunology; D000789:Angina, Unstable / Q000097:blood* / Q000175:diagnosis / Q000201:enzymology / Q000276:immunology; D015415:Biomarkers / Q000097:blood; D016009:Chi-Square Distribution; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D000067596:Interleukin-33; D007378:Interleukins / Q000097:blood*; D016015:Logistic Models; D008297:Male; D053505:Matrix Metalloproteinases, Secreted / Q000097:blood*; D008875:Middle Aged; D009203:Myocardial Infarction / Q000097:blood* / Q000175:diagnosis / Q000201:enzymology / Q000276:immunology; D011237:Predictive Value of Tests; D012189:Retrospective Studies; D012720:Severity of Illness Index
D015415:Biomarkers; C506536:IL33 protein, human; D000067596:Interleukin-33; D007378:Interleukins; C424663:MMP28 protein, human; D053505:Matrix Metalloproteinases, Secreted