Molecular characterization of ESBL-producing Enterobacteriaceae in northern Portugal.
Extended-spectrum β-lactamases (ESBLs) prevalence was studied in the north of Portugal, among 193 clinical isolates belonging to citizens in a district in the boundaries between this country and Spain from a total of 7529 clinical strains. In the present study we recovered some members of Enterobacteriaceae family, producing ESBL enzymes, including Escherichia coli (67.9%), Klebsiella pneumoniae (30.6%), Klebsiella oxytoca (0.5%), Enterobacter aerogenes (0.5%), and Citrobacter freundii (0.5%). β -lactamases genes blaTEM, blaSHV, and blaCTX-M were screened by polymerase chain reaction (PCR) and sequencing approaches. TEM enzymes were among the most prevalent types (40.9%) followed by CTX-M (37.3%) and SHV (23.3%). Among our sample of 193 ESBL-producing strains 99.0% were resistant to the fourth-generation cephalosporin cefepime. Of the 193 isolates 81.3% presented transferable plasmids harboring bla ESBL genes. Clonal studies were performed by PCR for the enterobacterial repetitive intragenic consensus (ERIC) sequences. This study reports a high diversity of genetic patterns. Ten clusters were found for E. coli isolates and five clusters for K. pneumoniae strains by means of ERIC analysis. In conclusion, in this country, the most prevalent type is still the TEM-type, but CTX-M is growing rapidly.
Ciências Químicas e das Biomoléculas (CQB) e Centro de Investigação em Saúde e Ambiente (CISA), Escola Superior de Tecnologia da Saúde do Porto (ESTSP), Instituto Politécnico do Porto (IPP), 4400-330 Porto, Portugal ; CFBQ-Centro de Farmacologia e Biopatologia Química (U38-FCT), Faculdade de Medicina do Porto, Porto, Portugal.
Rúben
0000-0001-8933-3984
R
Fernandes
Departamento de Ciências do Ambiente, Escola Superior Agrária de Coimbra, Instituto Politécnico de Coimbra, Coimbra, Portugal.
Paula
P
Amador
Departamento de Biologia Molecular, Instituto de Ciência Biomédicas de Abel Salazar (ICBAS), Universidade do Porto, Porto, Portugal.
Carla
C
Oliveira
Ciências Químicas e das Biomoléculas (CQB) e Centro de Investigação em Saúde e Ambiente (CISA), Escola Superior de Tecnologia da Saúde do Porto (ESTSP), Instituto Politécnico do Porto (IPP), 4400-330 Porto, Portugal ; CFBQ-Centro de Farmacologia e Biopatologia Química (U38-FCT), Faculdade de Medicina do Porto, Porto, Portugal ; Centro Hospitalar da Universidade de Coimbra (CHUC), Coimbra, Portugal.
Cristina
C
Prudêncio
D004755:Enterobacteriaceae / Q000201:enzymology / Q000235:genetics*; D007525:Isoelectric Focusing; D016133:Polymerase Chain Reaction; D011174:Portugal; D001618:beta-Lactamases / Q000235:genetics*
D001618:beta-Lactamases