Prostate stem cell antigen antibody-conjugated multiwalled carbon nanotubes for targeted ultrasound imaging and drug delivery.
Multiwalled carbon nanotubes (MWCNTs) are cut short and grafted with polyethylenimine (PEI) for further covalent conjugation to fluorescein isothiocyanate (FITC) and prostate stem cell antigen (PSCA) monoclonal antibody (mAb). The in vitro and in vivo toxicity data reveal that the as-prepared CNT-PEI(FITC)-mAb has good biocompatibility. Combined flow cytometry and confocal luminescence imaging experiments confirm that the CNT-PEI(FITC)-mAb can specifically target the cancer cells which overexpress PSCA. The results of in vitro and in vivo ultrasound (US) imaging indicate that CNT-PEI(FITC)-mAb has great potential to be used as a targeted US contrast agent. The in vivo anti-cancer efficacy testing using PC-3 tumor-bearing mice as animal models demonstrates that CNT-PEI(FITC)-mAb can targetedly deliver drug to the tumors and suppress tumor growth. Findings from this study suggest that the CNT-PEI(FITC)-mAb could be used as a multifunctional platform for simultaneous US imaging and drug delivery applications.
The Key Laboratory of Resource Chemistry of Ministry of Education and the Shanghai Key Laboratory of the Rare Earth Functional Materials, Department of Chemistry, College of Life and Environmental Science, Shanghai Normal University, Shanghai 200234, PR China. Electronic address: wuhuixia@shnu.edu.cn.
Huixia
H
Wu
The Key Laboratory of Resource Chemistry of Ministry of Education and the Shanghai Key Laboratory of the Rare Earth Functional Materials, Department of Chemistry, College of Life and Environmental Science, Shanghai Normal University, Shanghai 200234, PR China.
Haili
H
Shi
The Key Laboratory of Resource Chemistry of Ministry of Education and the Shanghai Key Laboratory of the Rare Earth Functional Materials, Department of Chemistry, College of Life and Environmental Science, Shanghai Normal University, Shanghai 200234, PR China.
Hao
H
Zhang
The Key Laboratory of Resource Chemistry of Ministry of Education and the Shanghai Key Laboratory of the Rare Earth Functional Materials, Department of Chemistry, College of Life and Environmental Science, Shanghai Normal University, Shanghai 200234, PR China.
Xue
X
Wang
The Key Laboratory of Resource Chemistry of Ministry of Education and the Shanghai Key Laboratory of the Rare Earth Functional Materials, Department of Chemistry, College of Life and Environmental Science, Shanghai Normal University, Shanghai 200234, PR China.
Yan
Y
Yang
The Key Laboratory of Resource Chemistry of Ministry of Education and the Shanghai Key Laboratory of the Rare Earth Functional Materials, Department of Chemistry, College of Life and Environmental Science, Shanghai Normal University, Shanghai 200234, PR China.
Chao
C
Yu
Department of Biology, College of Life and Environmental Science, Shanghai Normal University, Shanghai 200234, PR China.
Caiqin
C
Hao
Department of Ultrasound, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200127, PR China.
Jing
J
Du
The Key Laboratory of Resource Chemistry of Ministry of Education and the Shanghai Key Laboratory of the Rare Earth Functional Materials, Department of Chemistry, College of Life and Environmental Science, Shanghai Normal University, Shanghai 200234, PR China.
He
H
Hu
The Key Laboratory of Resource Chemistry of Ministry of Education and the Shanghai Key Laboratory of the Rare Earth Functional Materials, Department of Chemistry, College of Life and Environmental Science, Shanghai Normal University, Shanghai 200234, PR China. Electronic address: shipingy@shnu.edu.cn.
Shiping
S
Yang
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D000951:Antigens, Neoplasm / Q000276:immunology* / Q000378:metabolism; D000970:Antineoplastic Agents / Q000494:pharmacology; D045744:Cell Line, Tumor; D003952:Diagnostic Imaging; D004195:Disease Models, Animal; D016503:Drug Delivery Systems; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D058851:GPI-Linked Proteins / Q000276:immunology / Q000378:metabolism; D006801:Humans; D008297:Male; D051379:Mice; D037742:Nanotubes, Carbon / Q000737:chemistry*; D009363:Neoplasm Proteins / Q000276:immunology* / Q000378:metabolism; D011094:Polyethyleneimine / Q000737:chemistry; D011471:Prostatic Neoplasms / Q000000981:diagnostic imaging* / Q000188:drug therapy; D014463:Ultrasonography
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D000970:Antineoplastic Agents; D058851:GPI-Linked Proteins; D037742:Nanotubes, Carbon; D009363:Neoplasm Proteins; C110843:PSCA protein, human; D011094:Polyethyleneimine; D016650:Fluorescein-5-isothiocyanate
Antibody; Carbon nanotubes; Drug delivery; Prostate cancer; Ultrasound imaging