Comparison of high-intensity ultraviolet and NB-UVB on the maturation of melanocytes derived from hair follicle neural crest stem cells.
Both high-intensity ultraviolet and narrowband ultraviolet B (NB-UVB) are important therapeutic options for vitiligo management, but high-intensity ultraviolet is more effective than NB-UVB. However, the underlying mechanisms have not been well investigated. Herein, we compare the effects of high-intensity ultraviolet and NB-UVB on the pigmentation of melanocytes derived from hair follicle-derived neural crest stem cells (HF-NCSCs) in vitro and study the underlying mechanisms. The HF-NCSCs were isolated from mouse whisker follicles. After radiation with high-intensity ultraviolet and NB-UVB, respectively, the cell viability by the CCK-8 assay showed gradual inhibitory effects in a dose-dependent manner, which has no apparent difference between the two modalities. The mRNA for melanogenesis factors such as tyrosinase and tyrp1 of the differentiated melanocytes increased significantly with high-intensity ultraviolet compared to the same dose of NB-UVB exposure. Furthermore, the expression of Mc1r was significantly increased by high-intensity ultraviolet in contrast to NB-UVB at the dosage of 0.5 J. By and large, these data suggest that high-intensity ultraviolet exhibited greater efficiency on the maturation of the melanocyte lineage differentiated from HF-NCSCs compared to NB-UVB with the same dose, which was probably due to the stronger stimulatory action of Mc1r. This may provide new insights into the different efficacies of high-intensity ultraviolet and NB-UVB in the treatment of vitiligo repigmentation.
Department of Dermatology, Wuxi Second Affiliated Hospital of Nanjing Medical University, 68 Zhongshan Road, Wuxi, 214002, Jiangsu Province, China.
Dake
D
Dong
Shujun
S
Chen
Xiaoli
X
Zhang
Cheng
C
Jin
Yuan
Y
Zheng
Lijia
L
Yang
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation / Q000528:radiation effects*; D002470:Cell Survival / Q000528:radiation effects; D018859:Hair Follicle / Q000166:cytology*; D008544:Melanocytes / Q000166:cytology* / Q000378:metabolism / Q000528:radiation effects*; D008810:Mice, Inbred C57BL; D009432:Neural Crest / Q000166:cytology*; D044102:Receptor, Melanocortin, Type 1 / Q000378:metabolism; D055757:SOXE Transcription Factors / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology* / Q000528:radiation effects; D014466:Ultraviolet Rays; D000521:alpha-MSH / Q000378:metabolism
D015415:Biomarkers; D044102:Receptor, Melanocortin, Type 1; D055757:SOXE Transcription Factors; D000521:alpha-MSH