Prevalence, molecular characterization and zoonotic potential of Cryptosporidium spp. in goats in Henan and Chongqing, China.
To estimate the prevalence and public health significance of cryptosporidiosis in goats in China, 1265 fecal samples from seven farms in Henan province and Chongqing city were examined for Cryptosporidium oocysts. The overall infection rate of Cryptosporidium spp. was 3.48% (44/1256). Significant difference was observed among age groups, with the post weaned kids having the highest infection rate (4.58%; ρ<0.01). Cryptosporidium spp. were characterized by PCR-restriction fragment length polymorphism (RFLP) analysis and DNA sequence analysis of the small subunit (SSU) rRNA gene. The SSU rRNA-based PCR identified three Cryptosporidium species, including Cryptosporidium ubiquitum (24/44) in Henan and Chongqing, and Cryptosporidium andersoni (16/44) and Cryptosporidium xiaoi (4/44) in Henan. Among which, the C. ubiquitum and C. andersoni were first identified in goats thus far and were found in all age groups except no C. andersoni being found in the postparturition nannies, whereas the C. xiaoi was detected in pre-weaned kids and pregnant nannies. Subtyping C. ubiquitum by DNA sequence analysis of the 60 kDa glycoprotein (gp60) gene suggested the isolates identified all belonged to zoonotic XIIa subtype 2. Thus, the dominant C. ubiquitum found in this study and the XIIa subtype 2 has been found in humans indicated goats are a potential source for zoonotic infections with the C. ubiquitum. More studies are needed for better understanding of differences in the transmission and public health significance of cryptosporidiosis in goats.
College of Veterinary Medicine, China Agricultural University, Beijing 100193, China; College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China; International Joint Research Laboratory for Zoonotic Diseases of Henan, Zhengzhou 450002, China.
Rongjun
R
Wang
College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China; International Joint Research Laboratory for Zoonotic Diseases of Henan, Zhengzhou 450002, China.
Guoquan
G
Li
College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China; International Joint Research Laboratory for Zoonotic Diseases of Henan, Zhengzhou 450002, China.
Bin
B
Cui
College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China; International Joint Research Laboratory for Zoonotic Diseases of Henan, Zhengzhou 450002, China.
Jianying
J
Huang
College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China; International Joint Research Laboratory for Zoonotic Diseases of Henan, Zhengzhou 450002, China.
Zhaohui
Z
Cui
College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China; International Joint Research Laboratory for Zoonotic Diseases of Henan, Zhengzhou 450002, China.
Sumei
S
Zhang
College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China; International Joint Research Laboratory for Zoonotic Diseases of Henan, Zhengzhou 450002, China.
Haiju
H
Dong
College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China; International Joint Research Laboratory for Zoonotic Diseases of Henan, Zhengzhou 450002, China.
Daoyou
D
Yue
College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China; International Joint Research Laboratory for Zoonotic Diseases of Henan, Zhengzhou 450002, China. Electronic address: zhanglx8999@gmail.com.
Longxian
L
Zhang
College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China; International Joint Research Laboratory for Zoonotic Diseases of Henan, Zhengzhou 450002, China.
Changshen
C
Ning
College of Veterinary Medicine, China Agricultural University, Beijing 100193, China. Electronic address: vetdean@cau.edu.cn.
Ming
M
Wang
D017677:Age Distribution; D000818:Animals; D001483:Base Sequence; D002681:China / Q000453:epidemiology; D003457:Cryptosporidiosis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D003458:Cryptosporidium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D016054:DNA, Protozoan / Q000737:chemistry / Q000302:isolation & purification; D005243:Feces / Q000469:parasitology; D005260:Female; D060005:Genotyping Techniques / Q000662:veterinary; D015511:Goat Diseases / Q000453:epidemiology / Q000469:parasitology*; D006041:Goats; D008969:Molecular Sequence Data; D033661:Oocysts / Q000737:chemistry; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012150:Polymorphism, Restriction Fragment Length; D011247:Pregnancy; D015597:Pregnancy Complications, Parasitic / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary; D015995:Prevalence; D012335:RNA, Ribosomal / Q000235:genetics; D015183:Restriction Mapping / Q000662:veterinary; D015047:Zoonoses / Q000453:epidemiology / Q000469:parasitology
D016054:DNA, Protozoan; D012335:RNA, Ribosomal
C. andersoni; C. ubiquitum; C. xiaoi; Cryptosporidium; Goats; SSU rRNA; gp60