Impact of short-time urine freezing on the sensitivity of an established Schistosoma real-time PCR assay.
Urogenital schistosomiaisis is a serious public health problem in sub-Saharan Africa. In this study, we have updated an established real-time polymerase chain reaction (PCR) routinely used in our laboratory. Schistosoma genus-specific real-time PCR was performed on DNA isolated from 85 urine samples and pellets obtained after centrifugation without and after frozen storage. The results revealed that concentration by centrifugation of the urine samples and freezing of the samples before extracting DNA improves the sensitivity of the PCR.
Centre de Recherches Médicales de Lambaréné (CERMEL), Lambaréné, Hôpital Albert Schweitzer Gabon; Institut für Tropenmedizin, Universität Tübingen, Tübingen, Germany; Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands; Laboratory for Medical Microbiology and Immunology, St. Elisabeth Hospital, Tilburg, The Netherlands hkenguele@gmail.com.
Hilaire M
HM
Kenguele
Centre de Recherches Médicales de Lambaréné (CERMEL), Lambaréné, Hôpital Albert Schweitzer Gabon; Institut für Tropenmedizin, Universität Tübingen, Tübingen, Germany; Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands; Laboratory for Medical Microbiology and Immunology, St. Elisabeth Hospital, Tilburg, The Netherlands.
Ayola A
AA
Adegnika
Centre de Recherches Médicales de Lambaréné (CERMEL), Lambaréné, Hôpital Albert Schweitzer Gabon; Institut für Tropenmedizin, Universität Tübingen, Tübingen, Germany; Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands; Laboratory for Medical Microbiology and Immunology, St. Elisabeth Hospital, Tilburg, The Netherlands.
Anne-Marie
AM
Nkoma
Centre de Recherches Médicales de Lambaréné (CERMEL), Lambaréné, Hôpital Albert Schweitzer Gabon; Institut für Tropenmedizin, Universität Tübingen, Tübingen, Germany; Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands; Laboratory for Medical Microbiology and Immunology, St. Elisabeth Hospital, Tilburg, The Netherlands.
Ulysse
U
Ateba-Ngoa
Centre de Recherches Médicales de Lambaréné (CERMEL), Lambaréné, Hôpital Albert Schweitzer Gabon; Institut für Tropenmedizin, Universität Tübingen, Tübingen, Germany; Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands; Laboratory for Medical Microbiology and Immunology, St. Elisabeth Hospital, Tilburg, The Netherlands.
Mirabeau
M
Mbong
Centre de Recherches Médicales de Lambaréné (CERMEL), Lambaréné, Hôpital Albert Schweitzer Gabon; Institut für Tropenmedizin, Universität Tübingen, Tübingen, Germany; Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands; Laboratory for Medical Microbiology and Immunology, St. Elisabeth Hospital, Tilburg, The Netherlands.
Jeannot
J
Zinsou
Centre de Recherches Médicales de Lambaréné (CERMEL), Lambaréné, Hôpital Albert Schweitzer Gabon; Institut für Tropenmedizin, Universität Tübingen, Tübingen, Germany; Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands; Laboratory for Medical Microbiology and Immunology, St. Elisabeth Hospital, Tilburg, The Netherlands.
Bertrand
B
Lell
Centre de Recherches Médicales de Lambaréné (CERMEL), Lambaréné, Hôpital Albert Schweitzer Gabon; Institut für Tropenmedizin, Universität Tübingen, Tübingen, Germany; Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands; Laboratory for Medical Microbiology and Immunology, St. Elisabeth Hospital, Tilburg, The Netherlands.
Jaco J
JJ
Verweij
D000293:Adolescent; D000328:Adult; D000818:Animals; D002648:Child; D018509:DNA, Helminth / Q000652:urine*; D005260:Female; D005615:Freezing; D006801:Humans; D008297:Male; D010270:Parasite Egg Count; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012548:Schistosoma haematobium / Q000235:genetics / Q000302:isolation & purification*; D012553:Schistosomiasis haematobia / Q000175:diagnosis* / Q000469:parasitology; D012680:Sensitivity and Specificity; D013045:Species Specificity; D055815:Young Adult
D018509:DNA, Helminth