The frequency of invariant natural killer T cells correlates with the severity of myocarditis.
Invariant natural killer T cells (iNKT) perform different functions in different diseases. The cells were reported to protect myocarditis. However, the detail relationships between iNKT and Coxsackievirus B3 (CVB3)-induced myocarditis remain unclear. In order to investigate the correlation between the severity of CVB3-induced inflammation infiltration and the proportion of iNKT in the spleen and circulating blood, BALB/c mice were grouped into three groups according to the inflammation infiltration area of heart sections. The proportion of iNKT in CD3-positive cells in the spleen correlated negatively with the inflammation area (linear fit; R(2)=0.93) and virus capsid protein VP1 (linear fit; R(2)=0.84) in the myocardial tissue, while the proportion of iNKT in CD3-positive cells in the PBMC positively correlated with the inflammation area (linear fit; R(2)=0.91) and virus capsid protein VP1 (linear fit; R(2)=0.93) in the myocardial tissue. The results imply that iNKT might be used as a parameter for the diagnosis of myocarditis in clinical practice.
Key Laboratory of Viral Heart Diseases, Ministry of Public Health, Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University , Shanghai, China .
Xinggang
X
Wang
Minghui
M
Li
Yeqing
Y
Xie
Ying
Y
Yu
Guijian
G
Liu
Yong
Y
Yu
Xiangdong
X
Yang
Yunzeng
Y
Zou
Junbo
J
Ge
Ruizhen
R
Chen
D000818:Animals; D001769:Blood / Q000276:immunology; D017252:CD3 Complex / Q000032:analysis; D003384:Coxsackievirus Infections / Q000150:complications / Q000276:immunology* / Q000473:pathology* / Q000821:virology; D004195:Disease Models, Animal; D029822:Enterovirus B, Human / Q000276:immunology*; D006651:Histocytochemistry; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D009205:Myocarditis / Q000473:pathology*; D009206:Myocardium / Q000473:pathology; D055611:Natural Killer T-Cells / Q000737:chemistry / Q000276:immunology*; D012720:Severity of Illness Index; D013154:Spleen / Q000276:immunology
D017252:CD3 Complex