Regeneration of Aplysia bag cell neurons is synergistically enhanced by substrate-bound hemolymph proteins and laminin.
We have investigated Aplysia hemolymph as a source of endogenous factors to promote regeneration of bag cell neurons. We describe a novel synergistic effect between substrate-bound hemolymph proteins and laminin. This combination increased outgrowth and branching relative to either laminin or hemolymph alone. Notably, the addition of hemolymph to laminin substrates accelerated growth cone migration rate over ten-fold. Our results indicate that the active factor is either a high molecular weight protein or protein complex and is not the respiratory protein hemocyanin. Substrate-bound factor(s) from central nervous system-conditioned media also had a synergistic effect with laminin, suggesting a possible cooperation between humoral proteins and nervous system extracellular matrix. Further molecular characterization of active factors and their cellular targets is warranted on account of the magnitude of the effects reported here and their potential relevance for nervous system repair.
Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520.
Callen
C
Hyland
Departments of Mechanical Engineering & Materials Science, Chemical & Environmental Engineering, Physics, and Cell Biology, Yale University, New Haven, CT 06520.
Eric R
ER
Dufresne
Departments of Mechanical Engineering & Materials Science, Chemical & Environmental Engineering, Physics, and Cell Biology, Yale University, New Haven, CT 06520.
Eric R
ER
Dufrense
Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520.
Paul
P
Forscher
D000818:Animals; D001048:Aplysia / Q000502:physiology*; D002478:Cells, Cultured; D020439:Growth Cones / Q000502:physiology; D006433:Hemocyanins / Q000378:metabolism; D006458:Hemolymph / Q000378:metabolism*; D007797:Laminin / Q000378:metabolism*; D016501:Neurites / Q000502:physiology; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D012038:Regeneration; D013379:Substrate Specificity
D007797:Laminin; D006433:Hemocyanins