Electrochemical immunosensor based on hydrophilic polydopamine-coated prussian blue-mesoporous carbon for the rapid screening of 3-bromobiphenyl.
A sensitive electrochemical immunosensor for 3-bromobiphenyl (3-BBP) detection was constructed by employing a new polydopamine coated prussian blue-mesoporous carbon (PDOP/PB/CMK-3) nanocomposite as the substrate platform and multi-horseradish peroxidase-double helix carbon nanotubes-secondary antibody (multi-HRP-DHCNTs-Ab2) as the signal label. PB/CMK-3 was firstly successfully in-situ synthesized with the aid of the CMK-3 reduction, which was characterized by transmission electron microscope (TEM), infrared spectroscopy (IR), X-ray diffraction (XRD) and N2 adsorption-desorption analysis. By using PDOP/PB/CMK-3 as the substrate, it can effectively enhance the specific surface for antigen loading due to the three-dimensional structure of the nanocomposites, while large amount of PB that fixed inside or outside the pore of CMK-3 successfully improved the electrochemical response and the PDOP film can provide a biocompatible environment to maintain the activity of antigen availability. Under the optimized conditions, the proposed immunosensor shows a good current response to 3-BBP in a linear range from 5 pM to 2 nM with a detection limit of 2.25 pM. In addition, the specificity, reproducibility and stability of the immunosensor were also proved to be acceptable, indicating its potential application in environmental monitoring.
Institute of Biomaterials, College of Sciences, South China Agricultural University, Guangzhou 510642, China.
Zihong
Z
Sun
Institute of Biomaterials, College of Sciences, South China Agricultural University, Guangzhou 510642, China.
Zhigang
Z
Luo
Institute of Biomaterials, College of Sciences, South China Agricultural University, Guangzhou 510642, China.
Cuifen
C
Gan
Shenzhen Water Quality Center, Shenzhen 518036, China.
Shidong
S
Fei
Institute of Biomaterials, College of Sciences, South China Agricultural University, Guangzhou 510642, China; State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, China. Electronic address: liuyingju@hotmail.com.
Yingju
Y
Liu
The Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642, China.
Hongtao
H
Lei
D055910:Antibodies, Immobilized / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation; D001713:Biphenyl Compounds / Q000032:analysis*; D055664:Electrochemical Techniques / Q000295:instrumentation*; D004867:Equipment Design; D005295:Ferrocyanides / Q000737:chemistry*; D007118:Immunoassay / Q000295:instrumentation; D007211:Indoles / Q000737:chemistry*; D057230:Limit of Detection; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D011108:Polymers / Q000737:chemistry*; D016062:Porosity
D055910:Antibodies, Immobilized; D001713:Biphenyl Compounds; D005295:Ferrocyanides; D007211:Indoles; D037742:Nanotubes, Carbon; D011108:Polymers; C568283:polydopamine; C034949:4-bromobiphenyl; C000170:ferric ferrocyanide
3-Bromobiphenyl; Double helix carbon nanotubes; Immunosensor; Mesoporous carbon; Polydopamine