Neuroprotective effects of ginsenoside Rg1 on oxygen-glucose deprivation reperfusion in PC12 cells.
The present study was aimed to investigate the protective effects of ginsenoside Rg1 (GRg1), an important component of ginseng, in oxygen-glucose deprivation/reperfusion (OGDR) and to elucidate the related mechanisms. PC12 cells were used as the model of OGDR. GRg1 administration was started 12 h before OGD and lasted for 12 h. After OGD, the cells were incubated in drug-free and full culture medium under normoxic condition for 24 h. Cell viability was then measured using MTT assay. Cell morphology was studied under a microscope. The expressions of survivin, caspase-3 and Terminal dUTP nick-end labeling (TUNEL) were measured by immunocytology. Results showed that pretreatment with GRg1 significantly increased the viability and survivin expression, and decreased the expressions of caspase-3 and TUNEL in a dose-dependent manner. In addition, it dramatically increased the number of cells and improved the cellular morphology. These results demonstrate the effect of GRg1 in preventing OGDR-induced PC12 cell apoptosis and partly reveal the mechanisms of the protective effect. It is suggested that GRg1 has potential beneficial effects in ischemic diseases.
Sheng-Li
SL
Huang
Xi-Jing
XJ
He
Zong-Fang
ZF
Li
Lei
L
Lin
Bin
B
Cheng
D000818:Animals; D015687:Cell Hypoxia / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D004396:Coloring Agents; D036145:Ginsenosides / Q000494:pharmacology*; D005947:Glucose / Q000172:deficiency*; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D018696:Neuroprotective Agents; D016716:PC12 Cells; D051381:Rats; D013778:Tetrazolium Salts; D013844:Thiazoles
D004396:Coloring Agents; D036145:Ginsenosides; D018696:Neuroprotective Agents; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue; D005947:Glucose; C035054:ginsenoside Rg1