Delay in cleavage of porcine embryos after intracytoplasmic sperm injection (ICSI) shows poorer embryonic development.
In pigs, the embryonic developmental ability after intracytoplasmic sperm injection (ICSI) is inferior to that resulting from in vitro fertilization (IVF). We evaluated the timing of cell division up to blastocyst formation on embryonic development after ICSI using either whole sperm (w-ICSI) or the sperm head alone (h-ICSI) and IVF as a control. At 10 h after ICSI or IVF, we selected only zygotes, and each of the zygotes/embryos was evaluated for cleavage every 24 h until 168 h. We then observed a delay in the 1st and 2nd cleavages of h-ICSI embryos and also in blastocoele formation by w-ICSI embryos in comparison with IVF embryos. The rate of blastocyst formation and the quality of blastocysts in both ICSI groups were inferior to those in the IVF group. In conclusion, the delay in cleavage of porcine ICSI embryos shows poorer embryonic development.
Genetically Modified Organism Research Center, National Institute of Agrobiological Sciences, Ibaraki 305-0901, Japan.
Michiko
M
Nakai
Manabu
M
Ozawa
Naoki
N
Maedomari
Junko
J
Noguchi
Hiroyuki
H
Kaneko
Junya
J
Ito
Akira
A
Onishi
Naomi
N
Kashiwazaki
Kazuhiro
K
Kikuchi
D000818:Animals; D002478:Cells, Cultured; D002970:Cleavage Stage, Ovum / Q000502:physiology*; D046149:Embryo Culture Techniques; D004622:Embryo, Mammalian / Q000166:cytology*; D047108:Embryonic Development; D005260:Female; D005307:Fertilization in Vitro / Q000662:veterinary; D008297:Male; D020554:Sperm Injections, Intracytoplasmic / Q000009:adverse effects / Q000662:veterinary; D013552:Swine / Q000196:embryology*; D013997:Time Factors