A new method for yeast phagocytosis analysis by flow cytometry.
Herein we developed a method based on the quenching effect of propidium iodide over Sytox-Green fluorescence to assess yeast phagocytosis by flow cytometry. It allows accurate quantification of living from dead phagocytes; internalized from non-internalized cells, maintaining yeast fluorescence within phagocytes; and the different associations between phagocytes and fungal cells.
Centre of Molecular and Environmental Biology (CBMA), Department of Biology, University of Minho, Braga, Portugal.
Catarina
C
Carneiro
Centre of Molecular and Environmental Biology (CBMA), Department of Biology, University of Minho, Braga, Portugal.
Catarina
C
Vaz
Centre of Molecular and Environmental Biology (CBMA), Department of Biology, University of Minho, Braga, Portugal.
Joana
J
Carvalho-Pereira
Centre of Molecular and Environmental Biology (CBMA), Department of Biology, University of Minho, Braga, Portugal.
Célia
C
Pais
Centre of Molecular and Environmental Biology (CBMA), Department of Biology, University of Minho, Braga, Portugal. Electronic address: psampaio@bio.uminho.pt.
Paula
P
Sampaio
D000818:Animals; D002175:Candida / Q000166:cytology / Q000302:isolation & purification*; D002460:Cell Line; D005434:Flow Cytometry / Q000379:methods*; D008264:Macrophages / Q000502:physiology; D051379:Mice; D009930:Organic Chemicals; D010587:Phagocytosis / Q000502:physiology*; D011419:Propidium
D009930:Organic Chemicals; C402795:SYTOX Green; D011419:Propidium
Candida spp; Flow cytometry; PAMPs; Phagocytosis; Propidium iodide; Sytox Green