Glycated albumin: an overview of the In Vitro models of an In Vivo potential disease marker.
Glycation is a general spontaneous process in proteins which has significant impact on their physical and functional properties. These changes in protein properties could be related to several pathological consequences such as cataract, arteriosclerosis and Alzheimer's disease. Among the proteins, glycation of Human serum albumin (HSA) is of special interest. Human serum albumin is the most abundant protein in the plasma and because of its high sensitivity for glycation, undergoes structural and functional changes due to binding of reducing sugars in vitro. The glycation process occurs by plasma glucose in vivo which has great impacts on the three dimensional structure of protein. These changes are efficient and stable enough which makes the protein to be considered as a new special disease marker instead of HbA1C for diabetes. In some cases, glycated albumin was used as an alternative marker for glycemic control. Glycated albumin reacts with glucose ten times more rapidly than HbA1C and has shorter half-life which makes it more reliable for indicating glycemic states. In this review, glycation of Human Serum Albumin has been overviewed, starting from overall concepts of glycation, followed by some Examples of pathological consequences of protein glycation. The BSA aggregation was reviewed in terms of structural and biological impacts of glycation on the protein followed by reporting documents which indicate possibility of glycated albumin to be used as specific marker for diabetes. Finally, some of the studies related to the models of glycated albumin have been briefly described, with an emphasis on In vitro studies. It is interesting to note the relationship found between in vitro glycation experiments and the propensity of proteins to form amyloid structures, a point that could be further explored as to its significance in hyperglycemic states.
Department of Microbiology, Faculty of Science, Rasht Branch, Islamic Azad University, Rasht, Iran.
Amir
A
Arasteh
School of Biology, College of Science, University of Tehran, Tehran, Iran.
Sara
S
Farahi
School of Biology, College of Science, University of Tehran, Tehran, Iran.
Mehran
M
Habibi-Rezaei
Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.
Ali Akbar
AA
Moosavi-Movahedi
Diabetes; Disease marker; Glycation; Human serum albumin