A fluorescent probe for estimation of adenosine diphosphate and monitoring of glucose metabolism.
An ADP selective fluorescent probe working in aqueous medium was identified and the change in fluorescence as a function of ADP concentration was standardized. Using this probe, all the steps of glycolysis coupled with ATP/ADP inter-conversion and oxidative breakdown of pyruvate in the mitochondria were monitored and the consumption/production of ATP/ADP at each step was quantified. The quantity of ADP present in the mitochondria, taken from different body parts of a pig, was also determined. It is hypothesized that an appropriate modification of the technique may provide a diagnostic tool for monitoring biochemical pathways as well as for quick estimation of ADP in the mitochondria and other cell organelles.
UGC Sponsored Centre for Advanced Studies, Department of Chemistry, Guru Nanak Dev University, Amritsar-143005, India. palwinder_singh_2000@yahoo.com.
Arun
A
Kumar
Parteek
P
Prasher
Palwinder
P
Singh
D000244:Adenosine Diphosphate / Q000032:analysis*; D000818:Animals; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D005636:Fructosephosphates / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D005988:Glyceric Acids / Q000378:metabolism; D006019:Glycolysis; D008928:Mitochondria / Q000378:metabolism; D010084:Oxidation-Reduction; D010728:Phosphoenolpyruvate / Q000378:metabolism; D010766:Phosphorylation; D019289:Pyruvic Acid / Q000378:metabolism; D012996:Solutions; D034421:Sus scrofa
D005456:Fluorescent Dyes; D005636:Fructosephosphates; D005988:Glyceric Acids; D012996:Solutions; D000244:Adenosine Diphosphate; C027618:fructose-6-phosphate; D010728:Phosphoenolpyruvate; C005156:3-phosphoglycerate; D019289:Pyruvic Acid; D005947:Glucose