Aspergillus 6V4, a Strain Isolated from Manipueira, Produces High Amylases Levels by Using Wheat Bran as a Substrate.
The aim of this study was screening fungi strains, isolated from manipueira (a liquid subproduct obtained from the flour production of Manihot esculenta), for amylases production and investigating production of these enzymes by the strain Aspergillus 6V4. The fungi isolated from manipueira belonged to Ascomycota phylum. The strain Aspergillus 6V4 was the best amylase producer in the screening assay of starch hydrolysis in petri dishes (ASHPD) and in the assay in submerged fermentation (ASbF). The strain Aspergillus 6V4 produced high amylase levels (335 UI/L) using wheat bran infusion as the exclusive substrate and the supplementation of this substrate with peptone decreased the production of this enzyme. The moisture content of 70% was the best condition for the production of Aspergillus 6V4 amylases (385 IU/g) in solid state fermentation (SSF).
Universidade Federal do Amazonas, UFAM, Faculdade de Ciências Farmacêuticas, Manaus, AM, Brazil.
Jessyca Dos Reis
Jdos R
Celestino
Instituto Nacional de Pesquisas da Amazônia, INPA, Laboratório de Microbiologia Médica, Manaus, AM, Brazil.
Ana Caroline
AC
Duarte
Universidade Federal do Amazonas, UFAM, Faculdade de Ciências Farmacêuticas, Manaus, AM, Brazil.
Cláudia Maria de Melo
CM
Silva
Universidade Federal do Amazonas, UFAM, Faculdade de Ciências Farmacêuticas, Manaus, AM, Brazil.
Hellen Holanda
HH
Sena
Universidade Federal do Amazonas, UFAM, Faculdade de Ciências Farmacêuticas, Manaus, AM, Brazil.
Maria do Perpétuo Socorro Borges Carriço
Mdo P
Ferreira
Universidade Federal do Amazonas, UFAM, Faculdade de Ciências Farmacêuticas, Manaus, AM, Brazil.
Neila Hiraishi
NH
Mallmann
Universidade Federal do Amazonas, UFAM, Faculdade de Ciências Farmacêuticas, Manaus, AM, Brazil.
Natacha Pinheiro Costa
NP
Lima
Universidade Federal do Amazonas, UFAM, Faculdade de Ciências Farmacêuticas, Manaus, AM, Brazil.
Chanderlei de Castro
Cde C
Tavares
Universidade Federal do Amazonas, UFAM, Faculdade de Ciências Farmacêuticas, Manaus, AM, Brazil.
Rodrigo Otávio Silva
RO
de Souza
Universidade Estadual do Amazonas, UEA, Escola Superior de Tecnologia, Manaus, AM, Brazil.
Erica Simplício
ES
Souza
Instituto Nacional de Pesquisas da Amazônia, INPA, Laboratório de Microbiologia Médica, Manaus, AM, Brazil.
João Vicente Braga
JV
Souza