The effect of near-infrared MLS laser radiation on cell membrane structure and radical generation.
The therapeutic effects of low-power laser radiation of different wavelengths and light doses are well known, but the biochemical mechanism of the interaction of laser light with living cells is not fully understood. We have investigated the effect of MLS (Multiwave Locked System) laser near-infrared irradiation on cell membrane structure, functional properties, and free radical generation using human red blood cells and breast cancer MCF-4 cells. The cells were irradiated with low-intensity MLS near-infrared (simultaneously 808 nm, continuous emission and 905 nm, pulse emission, pulse-wave frequency, 1,000 or 2,000 Hz) laser light at light doses from 0 to 15 J (average power density 212.5 mW/cm(2), spot size was 3.18 cm(2)) at 22 °C, the activity membrane bound acetylcholinesterase, cell stability, anti-oxidative activity, and free radical generation were the parameters used in characterizing the structural and functional changes of the cell. Near-infrared low-intensity laser radiation changed the acetylcholinesterase activity of the red blood cell membrane in a dose-dependent manner: There was a considerable increase of maximal enzymatic rate and Michaelis constant due to changes in the membrane structure. Integral parameters such as erythrocyte stability, membrane lipid peroxidation, or methemoglobin levels remained unchanged. Anti-oxidative capacity of the red blood cells increased after MLS laser irradiation. This irradiation induced a time-dependent increase in free radical generation in MCF-4 cells. Low-intensity near-infrared MLS laser radiation induces free radical generation and changes enzymatic and anti-oxidative activities of cellular components. Free radical generation may be the mechanism of the biomodulative effect of laser radiation.
Clinic of Medical Rehabilitation, Medical University of Lodz, 75 Drewnowska Str., Lodz, 91-002, Poland, jolanta.kujawa@umed.lodz.pl.
Jolanta
J
Kujawa
Kamila
K
Pasternak
Ilya
I
Zavodnik
Robert
R
Irzmański
Dominika
D
Wróbel
Maria
M
Bryszewska
D000110:Acetylcholinesterase / Q000378:metabolism; D045744:Cell Line, Tumor; D021961:Cell Membrane Structures / Q000528:radiation effects*; D004912:Erythrocytes / Q000378:metabolism / Q000528:radiation effects; D005609:Free Radicals / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism; D006461:Hemolysis / Q000528:radiation effects; D006801:Humans; D007259:Infrared Rays; D007834:Lasers; D008706:Methemoglobin / Q000378:metabolism; D010084:Oxidation-Reduction / Q000528:radiation effects; D018384:Oxidative Stress / Q000528:radiation effects; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D005609:Free Radicals; D017392:Thiobarbituric Acid Reactive Substances; D008706:Methemoglobin; D000110:Acetylcholinesterase; D005978:Glutathione