Current impact and future directions of high throughput sequencing in plant virus diagnostics.
The ability to provide a fast, inexpensive and reliable diagnostic for any given viral infection is a key parameter in efforts to fight and control these ubiquitous pathogens. The recent developments of high-throughput sequencing (also called Next Generation Sequencing - NGS) technologies and bioinformatics have drastically changed the research on viral pathogens. It is now raising a growing interest for virus diagnostics. This review provides a snapshot vision on the current use and impact of high throughput sequencing approaches in plant virus characterization. More specifically, this review highlights the potential of these new technologies and their interplay with current protocols in the future of molecular diagnostic of plant viruses. The current limitations that will need to be addressed for a wider adoption of high-throughput sequencing in plant virus diagnostics are thoroughly discussed.
Laboratory of Phytopathology, University of Liège, Gembloux Agro-BioTech, Passage des déportés, 2, 5030 Gembloux, Belgium. Electronic address: sebastien.massart@ulg.ac.be.
Sebastien
S
Massart
Centro de Protección Vegetal, Instituto Valenciano de Investigaciones Agrarias (IVIA), Apartado Oficial, 46113 Moncada, Valencia, Spain.
Antonio
A
Olmos
Laboratory of Phytopathology, University of Liège, Gembloux Agro-BioTech, Passage des déportés, 2, 5030 Gembloux, Belgium.
Haissam
H
Jijakli
UMR 1332 de Biologie du fruit et Pathologie, INRA, CS20032, 33882 Villenave d'Ornon cedex, France; UMR 1332 de Biologie du fruit et Pathologie, Université de Bordeaux, CS20032, 33882 Villenave d'Ornon cedex, France.
Thierry
T
Candresse
D019295:Computational Biology / Q000379:methods / Q000639:trends; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods* / Q000639:trends; D010935:Plant Diseases / Q000821:virology*; D010942:Plant Viruses / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D010944:Plants / Q000821:virology*
High throughput sequencing; Next Generation Sequencing; Plant certification; Plant virus; Trends; Virus detection; Virus diagnostic