[A retrospective study of the relationship between bacterial numbers from central venous catheter tip cultures and blood cultures for evaluating central line-associated bloodstream infections].
Catheter-related bloodstream infection (CRBSI) is an infectious disease requiring special attention. It is a common cause of nosocomial infections; catheter insertion into the central veins particularly increases the risk of infection (CLA-BSI: central line-associated bloodstream infection). We examined the relationship between the number of bacterial colonies cultured from shredded central venous catheter (CVC) tips and from blood cultures in our hospital from 2011 to 2012. Coagulase-negative staphylococci topped the list of microbe isolated from the CVC tip culture, followed by Pseudomonas aeruginosa, Staphylococcus aureus, and Candida spp. S. aureus and Candida spp., with growth of over 15 colony-forming units in the CVC tip culture, were also detected at high rates in the blood culture. However, gramnegative bacilli (Enterobacteriaceae and P. aeruginosa) did not show a similar increase in colony number in the CVC tip culture. Because microbes adhering to shredded catheter tips are readily detected by culture, this method is useful as a routine diagnostic test. In addition, prompt clinical reporting of the bacterial number of serious CLA-BSI-causing S. aureus and Candida spp. isolated from CVC tips could contribute to earlier CLA-BSI diagnosis.
Division of Clinical Laboratory, Gifu University Hospital, 1-1 Yanagido, Gifu 501-1194, Japan.
Hirofumi
H
Ohtaki
Department of Microbiology, Gifu University Graduate School of Medicine, 1-1 Yanagido, Gifu 501-1194, Japan.
Kiyofumi
K
Ohkusu
Division of Clinical Laboratory, Gifu University Hospital, 1-1 Yanagido, Gifu 501-1194, Japan.
Asami
A
Nakayama
Division of Clinical Laboratory, Gifu University Hospital, 1-1 Yanagido, Gifu 501-1194, Japan.
Jun
J
Yonetamari
Division of Clinical Laboratory, Gifu University Hospital, 1-1 Yanagido, Gifu 501-1194, Japan.
Kohei
K
Ando
Division of Clinical Laboratory, Gifu University Hospital, 1-1 Yanagido, Gifu 501-1194, Japan.
Takashi
T
Miyazaki
Division of Clinical Laboratory, Gifu University Hospital, 1-1 Yanagido, Gifu 501-1194, Japan.
Hirotoshi
H
Ohta
Division of Clinical Laboratory, Gifu University Hospital, 1-1 Yanagido, Gifu 501-1194, Japan.
Nobuyuki
N
Furuta
Center for Nutritional Support and Infection Control, Gifu University Hospital, 1-1 Yanagido, Gifu 501-1194, Japan.
Tamayo
T
Watanabe
Department of Informative Clinical Medicine, Gifu University Graduate School of Medicine, 1-1 Yanagido, Gifu 501-1194, Japan.
Hiroyasu
H
Ito
Center for Nutritional Support and Infection Control, Gifu University Hospital, 1-1 Yanagido, Gifu 501-1194, Japan.
Nobuo
N
Murakami
Department of Informative Clinical Medicine, Gifu University Graduate School of Medicine, 1-1 Yanagido, Gifu 501-1194, Japan.
Mitsuru
M
Seishima
D058491:Bacterial Load; D001769:Blood / Q000382:microbiology*; D002175:Candida / Q000302:isolation & purification; D002405:Catheterization, Central Venous / Q000009:adverse effects* / Q000295:instrumentation*; D003428:Cross Infection / Q000209:etiology; D006801:Humans; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification; D012189:Retrospective Studies; D018805:Sepsis / Q000209:etiology*; D013211:Staphylococcus aureus / Q000302:isolation & purification