Polyaniline sheathed electrospun nanofiber bar for in vivo extraction of trace acidic phytohormones in plant tissue.
For in vivo extraction of trace and polar components in tissues, extraction materials should be biocompatible, highly permeable to matrix and have high extraction capacity for polar and ionic compounds. In this study, nanofibers (∼200nm) with rough polyaniline (PAn) sheath were prepared successfully for the first time by coaxial electrospinning and coelectrospun polymer sacrificing method. The nanofibers (0.5mg) were then compressed into tiny bar (Φ1mm×2mm) and inserted directly into the aloe leaf for in vivo extraction of trace phytohormones. Due to the large surface area (13.97m(2)/g) of nanofibers and large through pores formed between fibers, high extraction capacity (up to 10μg/bar) and permeability to tissue matrix of the tiny bar can be obtained. Moreover, PAn sheath has high adsorption equilibrium constants (10(7)-10(8)mL/mol) for acidic phytohormones in aloe leaf. Four phytohormones were detected by in vivo extraction with a spatial resolution of 3-8mm(3), temporal resolution of 20min, and limit of detection down to 60pg/g. The quantification results were well met with that by traditional in vitro organic solvent extraction.
Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, PR China; Dalian Institute of Chemical Physics, Graduate School of the Chinese Academy of Sciences, Beijing 100039, PR China.
Qian
Q
Wu
Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, PR China.
Dapeng
D
Wu
Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, PR China. Electronic address: guanyafeng@dicp.ac.cn.
Yafeng
Y
Guan
D000504:Aloe / Q000737:chemistry*; D000814:Aniline Compounds / Q000737:chemistry*; D057139:Nanofibers; D010937:Plant Growth Regulators / Q000302:isolation & purification*; D018515:Plant Leaves / Q000737:chemistry; D011108:Polymers
D000814:Aniline Compounds; D010937:Plant Growth Regulators; D011108:Polymers; C416807:polyaniline
Aloe leaf; Electrospun nanofibers; In vivo solid-phase micro-extraction; Phytohormones; Polyaniline