Dynamic encapsulation of hydrophilic nisin in hydrophobic poly (lactic acid) particles with controlled morphology by a single emulsion process.
Hydrophilic nisin-loaded hydrophobic poly (lactic acid) (PLA) particles with controlled size and shape were successfully produced utilizing a one-step single emulsification method. Preliminary shear stress and temperature tests showed that there was no significant loss in the nisin inhibition activity during this process. PLA/nisin composite particles were prepared into solid nanocomposite spheres (50-200 nm) or hollow microcomposite spheres (1-5 μm) under the operative conditions developed in our previous study, in which the hydrophilic nisin in the aqueous phase solution could be entrapped in the hydrophobic polymer in the emulsification process generating either single or multiple emulsions. The incorporation of nisin in PLA had little effect on key processing conditions, which allows the dynamic control of the morphology and property of resulting particles. Microscopic and surface analyses suggested that nisin was dispersed uniformly inside the polymer matrix and adsorbed on the particle surface. The encapsulation amount and efficiency were enhanced with the increase in nisin loading in the aqueous solution. Unique reversible control of particle size and shape by this process was successfully applied in the nisin encapsulation. Alternating temperature in the repeating emulsification steps improved the encapsulation efficiency while generated particles in desired size and shape.
Department of Chemical Engineering and Materials Science, Michigan State University, 2527 Engineering Building, East Lansing, MI 48824, USA.
Shaowen
S
Ji
Metna Co., 1926 Turner Street, Lansing, MI 48906, USA.
Jue
J
Lu
Department of Biosystems and Agricultural Engineering, Michigan State University, East Lansing, MI 48824, USA.
Zhiguo
Z
Liu
Department of Chemical Engineering and Materials Science, Michigan State University, 2527 Engineering Building, East Lansing, MI 48824, USA.
Devesh
D
Srivastava
Department of Chemical Engineering and Materials Science, Michigan State University, 2527 Engineering Building, East Lansing, MI 48824, USA.
Anna
A
Song
Department of Biosystems and Agricultural Engineering, Michigan State University, East Lansing, MI 48824, USA.
Yan
Y
Liu
Department of Chemical Engineering and Materials Science, Michigan State University, 2527 Engineering Building, East Lansing, MI 48824, USA. Electronic address: leeil@egr.msu.edu.
Ilsoon
I
Lee
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology; D004337:Drug Carriers / Q000737:chemistry*; D004655:Emulsions / Q000737:chemistry*; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry*; D008089:Listeria monocytogenes / Q000187:drug effects; D008088:Listeriosis / Q000188:drug therapy; D008863:Microspheres; D008969:Molecular Sequence Data; D009561:Nisin / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology; D010316:Particle Size; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*
D000900:Anti-Bacterial Agents; D004337:Drug Carriers; D004655:Emulsions; D011091:Polyesters; D011108:Polymers; D009561:Nisin; D019344:Lactic Acid; C033616:poly(lactide)
Emulsion process; Nisin encapsulation; PLA; Polymeric composites; Reversible morphology control