Notch ligand Delta-like 1 promotes the metastasis of melanoma by enhancing tumor adhesion.
Notch signaling plays a vital role in tumorigenicity and tumor progression by regulating proliferation, invasion, and the tumor microenvironment. Previous research by our group indicated that Notch ligand Delta-like 1 (Dll1) is involved in angiogenesis in melanoma, and we noticed that it took a longer time to trypsinize Dll1-expressing B16 melanoma cells than the control cells. In this article, we extended our study to investigate the effects of Dll1 on tumor cell adhesion and metastasis. Dll1 overexpression activated Notch signaling in B16 tumor cells and significantly enhanced the adhering capacity of B16 tumor cells both in vitro and in vivo. B16-Dll1 cells also had a higher metastatic potential than their counterpart in the mouse model of lung metastasis. Along with increased Dll1 expression, N-cadherin, but not E-cadherin, was upregulated in B16-Dll1 cells. These data suggested that Notch ligand Dll1 may enhance the adhesion and metastasis of melanoma cells by upregulation of N-cadherin.
Department of Orthopedic Surgery, Tangdu Hospital, The Fourth Military Medical University, Xi'an, China.
J P
JP
Zhang
Department of Oncology, Tangdu Hospital, The Fourth Military Medical University, Xi'an, China.
N
N
Li
Department of Orthopedic Surgery, Tangdu Hospital, The Fourth Military Medical University, Xi'an, China.
W Z
WZ
Bai
Department of Orthopedic Surgery, Tangdu Hospital, The Fourth Military Medical University, Xi'an, China.
X C
XC
Qiu
Department of Orthopedic Surgery, Tangdu Hospital, The Fourth Military Medical University, Xi'an, China.
B A
BA
Ma
Department of Orthopedic Surgery, Tangdu Hospital, The Fourth Military Medical University, Xi'an, China.
Y
Y
Zhou
Department of Orthopedic Surgery, Tangdu Hospital, The Fourth Military Medical University, Xi'an, China.
Q Y
QY
Fan
Department of Orthopedic Surgery, Tangdu Hospital, The Fourth Military Medical University, Xi'an, China.
L Q
LQ
Shan
D000818:Animals; D015153:Blotting, Western; D015820:Cadherins / Q000096:biosynthesis*; D002135:Calcium-Binding Proteins; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D015870:Gene Expression; D049452:Green Fluorescent Proteins; D061307:Human Umbilical Vein Endothelial Cells / Q000502:physiology; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism*; D008175:Lung Neoplasms / Q000556:secondary*; D008546:Melanoma, Experimental / Q000473:pathology / Q000556:secondary*; D008565:Membrane Proteins / Q000378:metabolism*; D008810:Mice, Inbred C57BL; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction / Q000235:genetics*; D015854:Up-Regulation
D015820:Cadherins; D002135:Calcium-Binding Proteins; C512941:DLK1 protein, human; D036341:Intercellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D049452:Green Fluorescent Proteins