Resistance training improves body composition and increases matrix metalloproteinase 2 activity in biceps and gastrocnemius muscles of diet-induced obese rats.
OBJECTIVE
We investigated the influence of resistance training on body composition and matrix metalloproteinase 2 activity in skeletal muscles of rats fed a high-fat diet.


METHODS
Thirty-two Wistar rats were divided into four experimental groups (n = 8/each) according to diet and exercise status: Control (standard diet), Obese Control (high-fat diet), Resistance Training (standard diet) and Obese Resistance Training (high-fat diet) groups. Animals were fed a high-fat diet for 12 weeks to promote excessive weight gain. Resistance Training groups performed 12 weeks of training periods after this period in a vertical ladder three times/week. Fat percentage, fat-free mass and fat mass were assessed using dual-energy X-ray absorptiometry, and matrix metalloproteinase 2 activity in biceps and gastrocnemius muscles was analyzed using zymography.


RESULTS
Resistance training significantly reduced body and fat masses and fat percentages in both trained groups (p<0.05). The maximal carrying load between trained groups was not different, but relative force was higher in the Resistance Training group (p<0.05). Of note, increased matrix metalloproteinase 2 activity was noted in the tested muscles of both trained groups (p<0.05).


CONCLUSIONS
In conclusion, altered body composition and muscle matrix metalloproteinase 2 activity promoted by excessive weight gain were positively modified by resistance training.
Department of Physiological Sciences, Laboratory of Exercise Physiology, Federal University of São Carlos, São Carlos, SP, Brazil.
Markus Vinicius Campos
MV
Souza
Department of Physical Education, Federal University of Maranhão, São Luis, MA, Brazil.
Richard Diego
RD
Leite
Department of Physiological Sciences, Laboratory of Exercise Physiology, Federal University of São Carlos, São Carlos, SP, Brazil.
Anderson Diogo de
AD
Souza Lino
University of Brasilia, Brasilia, DF, Brazil.
Rita de Cássia
Rde C
Marqueti
METROCAMP - IBMEC Educational Group and Catholic University of Campinas, Campinas, SP, Brazil.
Celene Fernandes
CF
Bernardes
Department of Physiological Sciences, Laboratory of Biochemistry and Molecular Biology, Federal University of São Carlos, São Carlos, SP, Brazil.
Heloisa Sobreiro Selistre de
HS
Araújo
Eliete
E
Bouskela
Clinical and Experimental Research Laboratory in Vascular Biology (Biovasc), State University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
Eliete
E
Bouskella
Department of Physiological Sciences, Laboratory of Exercise Physiology, Federal University of São Carlos, São Carlos, SP, Brazil.
Gilberto Eiji
GE
Shiguemoto
Department of Physiological Sciences, Laboratory of Exercise Physiology, Federal University of São Carlos, São Carlos, SP, Brazil.
Sérgio Eduardo de
SE
Andrade Perez
Clinical and Experimental Research Laboratory in Vascular Biology (Biovasc), State University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
Luiz Guilherme
LG
Kraemer-Aguiar
D015502:Absorptiometry, Photon; D000818:Animals; D001823:Body Composition / Q000502:physiology*; D059305:Diet, High-Fat; D005260:Female; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000201:enzymology*; D009765:Obesity / Q000201:enzymology / Q000503:physiopathology*; D010805:Physical Conditioning, Animal; D011897:Random Allocation; D017208:Rats, Wistar; D015203:Reproducibility of Results; D055070:Resistance Training / Q000379:methods*; D013997:Time Factors
D020778:Matrix Metalloproteinase 2