Comparison of ligation-mediated PCR methods in differentiation of Mycobacterium tuberculosis strains.
Fast and inexpensive identification of epidemiological links between limited number of Mycobacterium tuberculosis strains is required to initially evaluate hospital outbreaks, laboratory crosscontaminations, and family or small community transmissions. The ligation-mediated PCR methods (LM-PCR) appear sufficiently discriminative and reproducible to be considered as a good candidate for such initial, epidemiological analysis. Here, we compared the discriminative power of the recently developed in our laboratory fast ligation amplification polymorphism (FLAP) method with fast ligation-mediated PCR (FLiP). Verification of the results was based on analyzing a set of reference strains and RFLP-IS6110 typing. The HGDI value was very similar for both LM-PCR methods and RFLP-IS6110 typing. However, only 52% of strains were correspondingly grouped by both FLiP and FLAP methods. Differentiation by FLAP method demonstrated a limited similarity to IS6110-RFLP (37,7%). As much as 78,7% of strains were grouped identically when differentiated by FLiP and IS6110-RFLP methods. The analysis differentiated 31, 35, and 36 groups when using FLAP, FLiP, and RFLP-IS6110 methods, respectively.
Department of Biochemistry and Cell Biology, University of Rzeszow, 35-601 Rzeszow, Poland.
Anna
A
Zaczek
Institute of Medical Biology, Polish Academy of Science, 93-232 Lodz, Poland.
Anna
A
Brzostek
Institute of Medical Biology, Polish Academy of Science, 93-232 Lodz, Poland.
Arkadiusz
A
Wojtasik
Department of Microbial Genetics, Faculty of Biology and Environmental Protection, University of Lodz, 90-237 Lodz, Poland.
Anna
A
Sajduda
Department of Biochemistry and Cell Biology, University of Rzeszow, 35-601 Rzeszow, Poland ; Institute of Medical Biology, Polish Academy of Science, 93-232 Lodz, Poland.
Jaroslaw
0000-0003-1427-884X
J
Dziadek
D006801:Humans; D009169:Mycobacterium tuberculosis / Q000502:physiology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D014376:Tuberculosis / Q000175:diagnosis* / Q000382:microbiology*