Nucleic acid detection technologies and marker molecules in bacterial diagnostics.
There is a growing need for quick and reliable methods for microorganism detection and identification worldwide. Although traditional culture-based technologies are trustworthy and accurate at a relatively low cost, they are also time- and labor-consuming and are limited to culturable bacteria. Those weaknesses have created a necessity for alternative technologies that are capable for faster and more precise bacterial identification from medical, food or environmental samples. The most common current approach is to analyze the nucleic acid component of analyte solution and determine the bacterial composition according to the specific nucleic acid profiles that are present. This review aims to give an up-to-date overview of different nucleic acid target sequences and respective analytical technologies.
Department of Biotechnology, IMCB, University of Tartu, Riia 23, Tartu 51010, Estonia.
Ott
O
Scheler
Barry
B
Glynn
Ants
A
Kurg
D000818:Animals; D001424:Bacterial Infections / Q000175:diagnosis* / Q000382:microbiology; D015415:Biomarkers / Q000378:metabolism; D015374:Biosensing Techniques; D004269:DNA, Bacterial / Q000235:genetics; D016680:Genome, Bacterial; D006801:Humans; D025202:Molecular Diagnostic Techniques; D020411:Oligonucleotide Array Sequence Analysis; D012329:RNA, Bacterial / Q000235:genetics
D015415:Biomarkers; D004269:DNA, Bacterial; D012329:RNA, Bacterial