Analytical performance and clinical decision limit of a new release for cardiac troponin I assay.
BACKGROUND
Cardiac troponins (cTns) are the 'gold standard' biomarker for the diagnosis and prognosis of acute coronary syndrome. Analytical performance is critical at low concentrations of cTn, and many of the current assays do not meet the guideline requirement of a 10% coefficient of variation (CV) at the 99th percentile concentrations. The aim of the study was to establish if the newly released Access® AccuTnI®+3 (AccuTnI+3) cardiac troponin I assay (Beckman Coulter Inc., Brea, CA, USA) reached this objective.


METHODS
All AccuTnI+3 assays were performed on UniCel® DxI800 analyzer (Beckman Coulter Inc). Limit of Blank (LoB), Limit of Detection (LoD) and Limit of Quantitation (LoQ) were determined according to Clinical Laboratory Standard Institute EP17-A and EP5-A2 protocols. The 99th percentile upper reference limit (URL) was determined by analysing serum samples from 330 apparently healthy blood donors (260 men, 70 women, age range 18-70 years, median age 36 years).


RESULTS
LoB and LoD values were 2.6 and 12 ng/L, respectively. The 10% CV was at 18 ng/L (95% confidence interval [CI] 8-25). The 99th percentile URL was 22 ng/L (95% CI 11-34).


CONCLUSIONS
The newly released assay has improved low-end analytical performance and reaches the goal of having a total imprecision ≤ 10% at 99th percentile of a healthy reference population (guideline acceptable). With this assay, it is now possible to utilize the 99th percentile as decision level for myocardial injury detection.
Clinical Pathology Laboratory, Azienda Ospedaliera Ospedali Riuniti Marche Nord, Fano, Pesaro (PU), Italy marcomoretti18@libero.it.
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Moretti
Clinical Pathology Laboratory, Azienda Ospedaliera Ospedali Riuniti Marche Nord, Fano, Pesaro (PU), Italy.
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Pieretti
Dipartimento SFS, Sezione di Biostatistica, Università "Carlo Bo", Urbino (PU), Italy.
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Sisti
Dipartimento SFS, Sezione di Biostatistica, Università "Carlo Bo", Urbino (PU), Italy.
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Rocchi
Clinical Pathology Laboratory, Azienda Ospedaliera Ospedali Riuniti Marche Nord, Fano, Pesaro (PU), Italy.
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Gasperoni
D054058:Acute Coronary Syndrome / Q000097:blood* / Q000175:diagnosis; D000293:Adolescent; D000328:Adult; D000368:Aged; D057205:Automation, Laboratory; D001681:Biological Assay / Q000592:standards*; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D057230:Limit of Detection; D008297:Male; D008875:Middle Aged; D012016:Reference Values; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D019210:Troponin I / Q000097:blood*
D015415:Biomarkers; D019210:Troponin I
Access® AccuTnI®+3 assay; EP17-A; EP5-A; cTnI; cardiology; decision limit; immunoassay; low end; troponin