Soil mesocosm studies on atrazine bioremediation.
Accumulation of pesticides in the environment causes serious issues of contamination and toxicity. Bioremediation is an ecologically sound method to manage soil pollution, but the bottleneck here, is the successful scale-up of lab-scale experiments to field applications. This study demonstrates pilot-scale bioremediation in tropical soil using atrazine as model pollutant. Mimicking field conditions, three different bioremediation strategies for atrazine degradation were explored. 100 kg soil mesocosms were set-up, with or without atrazine application history. Natural attenuation and enhanced bioremediation were tested, where augmentation with an atrazine degrading consortium demonstrated best pollutant removal. 90% atrazine degradation was observed in six days in soil previously exposed to atrazine, while soil without history of atrazine use, needed 15 days to remove the same amount of amended atrazine. The bacterial consortium comprised of 3 novel bacterial strains with different genetic atrazine degrading potential. The progress of bioremediation was monitored by measuring the levels of atrazine and its intermediate, cyanuric acid. Genes from the atrazine degradation pathway, namely, atzA, atzB, atzD, trzN and trzD were quantified in all mesocosms for 60 days. The highest abundance of all target genes was observed on the 6th day of treatment. trzD was observed in the bioaugmented mesocosms only. The bacterial community profile in all mesocosms was monitored by LH-PCR over a period of two months. Results indicate that the communities changed rapidly after inoculation, but there was no drastic change in microbial community profile after 1 month. Results indicated that efficient bioremediation of atrazine using a microbial consortium could be successfully up-scaled to pilot scale.
Environmental Genomics Division, National Environmental Engineering Research Institute (CSIR-NEERI), Nehru Marg, Nagpur 440 020, India.
Sneha
S
Sagarkar
Finnish Environment Institute, PO Box 140, FI-00251 Helsinki, Finland.
Aura
A
Nousiainen
Environmental Genomics Division, National Environmental Engineering Research Institute (CSIR-NEERI), Nehru Marg, Nagpur 440 020, India.
Shraddha
S
Shaligram
Finnish Environment Institute, PO Box 140, FI-00251 Helsinki, Finland.
Katarina
K
Björklöf
Department of Food and Environmental Sciences, Division of Microbiology, University of Helsinki, FI-00014 Helsinki, Finland.
Kristina
K
Lindström
Finnish Environment Institute, PO Box 140, FI-00251 Helsinki, Finland.
Kirsten S
KS
Jørgensen
Environmental Genomics Division, National Environmental Engineering Research Institute (CSIR-NEERI), Nehru Marg, Nagpur 440 020, India. Electronic address: a_kapley@neeri.res.in.
Atya
A
Kapley
D001280:Atrazine / Q000032:analysis / Q000378:metabolism*; D001419:Bacteria / Q000235:genetics; D001673:Biodegradation, Environmental; D004269:DNA, Bacterial / Q000032:analysis; D006540:Herbicides / Q000032:analysis / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000032:analysis / Q000378:metabolism*; D014227:Triazines / Q000032:analysis
D004269:DNA, Bacterial; D006540:Herbicides; D012989:Soil Pollutants; D014227:Triazines; C004632:cyanuric acid; D001280:Atrazine
Atrazine; Bioremediation; Consortium; LH-PCR; Mesocosm