Firing modes of dopamine neurons drive bidirectional GIRK channel plasticity.
G-protein-coupled inwardly rectifying potassium (GIRK) channels contribute to the resting membrane potential of many neurons, including dopamine (DA) neurons in the ventral tegmental area (VTA). VTA DA neurons are bistable, firing in two modes: one characterized by bursts of action potentials, the other by tonic firing at a lower frequency. Here we provide evidence that these firing modes drive bidirectional plasticity of GIRK channel-mediated currents. In acute midbrain slices of mice, we observed that in vitro burst activation of VTA DA neurons potentiated GIRK currents whereas tonic firing depressed these currents. This plasticity was not specific to the metabotropic receptor activating the GIRK channels, as direct activation of GIRK channels by nonhydrolyzable GTP also potentiated the currents. The plasticity of GIRK currents required NMDA receptor and CaMKII activation, and involved protein trafficking through specific PDZ domains of GIRK2c and GIRK3 subunit isoforms. Prolonged tonic firing may thus enhance the probability to switch into burst-firing mode, which then potentiates GIRK currents and favors the return to baseline. In conclusion, activity-dependent GIRK channel plasticity may represent a slow destabilization process favoring the switch between the two firing modes of VTA DA neurons.
Department of Basis Neurosciences, Medical Faculty, University of Geneva, CH-1211 Geneva, Switzerland, Peptide Biology Laboratories, Salk Institute for Biological Studies, La Jolla, California 92037, Department of Neuroscience, Icahn School of Medicine at Mount Sinai, New York, New York 10029, and Department of Clinical Neurosciences, Clinic of Neurology, Geneva University Hospital, CH-1211 Geneva, Switzerland.
Arnaud L
AL
Lalive
Michaelanne B
MB
Munoz
Camilla
C
Bellone
Paul A
PA
Slesinger
Christian
C
Lüscher
Kelly R
KR
Tan
D000200:Action Potentials; D000818:Animals; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2 / Q000378:metabolism; D059290:Dopaminergic Neurons / Q000378:metabolism / Q000502:physiology*; D005260:Female; D051676:G Protein-Coupled Inwardly-Rectifying Potassium Channels / Q000378:metabolism*; D006160:Guanosine Triphosphate / Q000378:metabolism; D053444:Inhibitory Postsynaptic Potentials; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009473:Neuronal Plasticity; D020033:Protein Isoforms / Q000378:metabolism; D021381:Protein Transport; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism; D017557:Ventral Tegmental Area / Q000166:cytology / Q000378:metabolism / Q000502:physiology
D051676:G Protein-Coupled Inwardly-Rectifying Potassium Channels; D020033:Protein Isoforms; D016194:Receptors, N-Methyl-D-Aspartate; D006160:Guanosine Triphosphate; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2
G-protein-coupled receptors; GIRK channels; dopamine neuron; phasic/tonic firing; plasticity; slow inhibition