HPLC-ED of low-molecular weight brominated phenols and tetrabromobisphenol A using pretreated carbon fiber microelectrode.
Electrochemically pretreated carbon fiber microelectrode was used to develop a simple, fast and sensitive HPLC-ECD method for the determination of brominated phenols. In addition to simple mono-, di- and tri-bromophenols (4-bromophenol, 2,4-dibromophenol, 2,6-dibromophenol, 2,4,6-tri-bromophenol) the possibility of electrochemical detection of 3,3',5,5'-tetrabromobisphenol A in oxidation mode is reported for the first time. The isocratic separation was achieved within 14 min using ternary mobile phase consisting of 50mM-phosphate buffer (pH 3.5), acetonitrile and methanol (35/15/50, v/v), and detection potential of E=+1450 mV (vs. Ag/AgCl). The carbon fiber microelectrode permitted to use high anodic potentials (up to +1800 mV vs. Ag/AgCl), the optimum analytical response was achieved at +1450 mV vs. Ag/AgCl. The limits of detection (LOD) for the studied analytes were within the range of 1.8-56.6 ng mL(-1). The developed method was applied to determination of brominated phenols in spiked water samples. Furthermore, after simple extraction with methyl tert-butyl ether, it was possible to quantify tetrabromobisphenol A (TBBA) in a piece of CRT monitor plastic casing. The found amount of TBBA was 10.22 mg kg(-1) (±0.43).
Department of Analytical Chemistry, Palacky University, Faculty of Science, 17. listopadu 12, CZ-771 46 Olomouc, Czech Republic.
Zdenka
Z
Bartosova
Department of Analytical Chemistry, Palacky University, Faculty of Science, 17. listopadu 12, CZ-771 46 Olomouc, Czech Republic. Electronic address: david.jirovsky@upol.cz.
David
D
Jirovsky
Department of Analytical Chemistry, Palacky University, Faculty of Science, 17. listopadu 12, CZ-771 46 Olomouc, Czech Republic.
Daniel
D
Riman
Department of Physical Chemistry, Palacky University, Faculty of Science, 17. listopadu 12, CZ-771 46 Olomouc, Czech Republic.
Vladimir
V
Halouzka
Department of Analytical Chemistry, Palacky University, Faculty of Science, 17. listopadu 12, CZ-771 46 Olomouc, Czech Republic.
Martin
M
Svidrnoch
Department of Physical Chemistry, Palacky University, Faculty of Science, 17. listopadu 12, CZ-771 46 Olomouc, Czech Republic.
Jan
J
Hrbac
D002244:Carbon / Q000737:chemistry*; D000077482:Carbon Fiber; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D055664:Electrochemical Techniques / Q000379:methods; D008839:Microelectrodes; D008970:Molecular Weight; D010636:Phenols / Q000032:analysis*; D011075:Polybrominated Biphenyls / Q000032:analysis*
D000077482:Carbon Fiber; D010636:Phenols; D011075:Polybrominated Biphenyls; C038964:2,6-dibromophenol; D002244:Carbon; C020806:tetrabromobisphenol A; C013930:2,4-dibromophenol; C032037:4-bromophenol
Brominated phenols; Carbon fiber; Electrochemical detection; Flame retardants; HPLC