Simple and sure methodology for massive hepatectomy in the mouse.
BACKGROUND
Reliable models for massive hepatectomy in the mouse are required for experimental liver research.


METHODS
We analyzed anatomical findings in 100 mice following massive hepatectomy induced by liver reduction >70%. The impact of various factors in the different models was also analyzed, including learning curves, operative time, survival curves and histopathological findings.


RESULTS
According to anatomical results, murine models with 75%, 80% and 90% of liver resection produced massive hepatectomy. Learning curves and operative times were most optimal with the clip technique. Each hepatectomy performed using the clip technique produced a reasonable survival curve, and there were no differences in histopathological findings between the suture and clip techniques.


CONCLUSIONS
Massive hepatectomy by the clip technique is simple and can provide reliable and relevant data.
Department of Neuroscience, Mayo Clinic in Florida, Jacksonville, FL 32224, USA (Tomohide Hori, Norifumi Ohashi, Feng Chen, Ann-Marie T. Baine, Lindsay B. Gardner, Sura Jermanus).
Tomohide
T
Hori
Department of Neuroscience, Mayo Clinic in Florida, Jacksonville, FL 32224, USA (Tomohide Hori, Norifumi Ohashi, Feng Chen, Ann-Marie T. Baine, Lindsay B. Gardner, Sura Jermanus).
Norifumi
N
Ohashi
Department of Neuroscience, Mayo Clinic in Florida, Jacksonville, FL 32224, USA (Tomohide Hori, Norifumi Ohashi, Feng Chen, Ann-Marie T. Baine, Lindsay B. Gardner, Sura Jermanus).
Feng
F
Chen
Department of Neuroscience, Mayo Clinic in Florida, Jacksonville, FL 32224, USA (Tomohide Hori, Norifumi Ohashi, Feng Chen, Ann-Marie T. Baine, Lindsay B. Gardner, Sura Jermanus).
Ann-Marie T
AM
Baine
Department of Neuroscience, Mayo Clinic in Florida, Jacksonville, FL 32224, USA (Tomohide Hori, Norifumi Ohashi, Feng Chen, Ann-Marie T. Baine, Lindsay B. Gardner, Sura Jermanus).
Lindsay B
LB
Gardner
Department of Neuroscience, Mayo Clinic in Florida, Jacksonville, FL 32224, USA (Tomohide Hori, Norifumi Ohashi, Feng Chen, Ann-Marie T. Baine, Lindsay B. Gardner, Sura Jermanus).
Sura
S
Jermanus
Division of Transplant Surgery, Department of Transplantation, Mayo Clinic in Florida, Jacksonville, FL 32224, USA (Justin H. Nguyen).
Justin H
JH
Nguyen
Hepatectomy; animal model; clip; microsurgery; mouse; surgical technique