Surfactant protein SP-D modulates activity of immune cells: proteomic profiling of its interaction with eosinophilic cells.
Surfactant protein D (SP-D), a C-type lectin, is known to protect against lung infection, allergy and inflammation. Its recombinant truncated form comprising homotrimeric neck and CRD region (rhSP-D) has been shown to bring down specific IgE levels, eosinophilia and restore Th2-Th1 homeostasis in murine models of lung hypersensitivity. SP-D knockout mice show intrinsic hypereosinophilia and airway hyper-responsiveness that can be alleviated by rhSP-D. The rhSP-D can bind activated eosinophils, inhibit chemotaxis and degranulation, and selectively induce oxidative burst and apoptosis in sensitized eosinophils. A global proteomics study of rhSP-D-treated eosinophilic cell line AML14.3D10 identified large-scale molecular changes associated with oxidative burst, cell stress and survival-related proteins potentially responsible for apoptosis induction. The data also suggested an involvement of RNA binding- and RNA splicing-related proteins. Thus, the proteomics approach yielded a catalog of differentially expressed proteins that may be protein signatures defining mechanisms of SP-D-mediated maintenance of homeostasis during allergy.
CSIR Institute of Genomics and Integrative Biology, Mall road, Delhi 110007, India.
Lakshna
L
Mahajan
Poonam
P
Gautam
Eswari
E
Dodagatta-Marri
Taruna
T
Madan
Uday
U
Kishore
D056704:Adaptive Immunity; D000818:Animals; D004802:Eosinophilia / Q000276:immunology / Q000378:metabolism; D004804:Eosinophils / Q000378:metabolism*; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology / Q000378:metabolism; D007113:Immunity, Innate; D015470:Leukemia, Myeloid, Acute / Q000276:immunology / Q000378:metabolism; D020543:Proteome / Q000378:metabolism*; D037663:Pulmonary Surfactant-Associated Protein D / Q000378:metabolism*; D011994:Recombinant Proteins / Q000494:pharmacology
D020543:Proteome; D037663:Pulmonary Surfactant-Associated Protein D; D011994:Recombinant Proteins
SP-D; allergy; apoptosis; eosinophilia; heterogeneous nuclear ribonucleoproteins; p53 pathway; proteomics; surfactant