A new series of vectors for constitutive, inducible or repressible gene expression in Candida guilliermondii.
The biotechnological potential of C. guilliermondii is now well established. This yeast species currently benefits from the availability of a convenient molecular toolbox including recipient strains, selectable markers and optimized transformation protocols. However, the number of expression systems for biotechnological applications in C. guilliermondii remains limited. We have therefore developed and characterized a new series of versatile controllable expression vectors for this yeast. While previous studies firmly demonstrated that knock-out systems represent efficient genetic strategies to interrupt yeast biochemical pathways at a specific step in C. guilliermondii, the set of expression plasmids described in this study will provide new powerful opportunities to boost homologous or heterologous biosynthetic routes by fine controlled over-expression approaches.
Université François-Rabelais, EA2106, Biomolécules et Biotechnologies Végétales, Tours, France.
Tatiana A
TA
Defosse
Université François-Rabelais, EA2106, Biomolécules et Biotechnologies Végétales, Tours, France.
Céline
C
Melin
Université François-Rabelais, EA2106, Biomolécules et Biotechnologies Végétales, Tours, France; Laboratorio de Biotecnología, Sede de Investigación Universitaria, Universidad de Antioquia, Colombia.
Erika J
EJ
Obando Montoya
Université François-Rabelais, EA2106, Biomolécules et Biotechnologies Végétales, Tours, France.
Arnaud
A
Lanoue
Université François-Rabelais, EA2106, Biomolécules et Biotechnologies Végétales, Tours, France.
Emilien
E
Foureau
Université François-Rabelais, EA2106, Biomolécules et Biotechnologies Végétales, Tours, France.
Gaëlle
G
Glévarec
Université François-Rabelais, EA2106, Biomolécules et Biotechnologies Végétales, Tours, France.
Audrey
A
Oudin
School of Biological Sciences, Wivenhoe Park, University of Essex, Colchester, United Kingdom.
Andrew J
AJ
Simkin
Université François-Rabelais, EA2106, Biomolécules et Biotechnologies Végétales, Tours, France.
Joël
J
Crèche
Laboratorio de Biotecnología, Sede de Investigación Universitaria, Universidad de Antioquia, Colombia.
Lucia
L
Atehortùa
Université François-Rabelais, EA2106, Biomolécules et Biotechnologies Végétales, Tours, France.
Nathalie
N
Giglioli-Guivarc'h
Université François-Rabelais, EA2106, Biomolécules et Biotechnologies Végétales, Tours, France.
Marc
M
Clastre
Université François-Rabelais, EA2106, Biomolécules et Biotechnologies Végétales, Tours, France.
Vincent
V
Courdavault
Université François-Rabelais, EA2106, Biomolécules et Biotechnologies Végétales, Tours, France. Electronic address: nicolas.papon@univ-tours.fr.
Nicolas
N
Papon
D002175:Candida / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular / Q000379:methods; D015870:Gene Expression / Q000235:genetics*; D005818:Genetic Engineering / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics*; D007763:Lac Operon; D010957:Plasmids / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
D011994:Recombinant Proteins
Candida guilliermondii; Constitutive promoter; Expression vectors; Inducible promoter; Repressible promoter