Chromium downregulates the expression of Acetyl CoA Carboxylase 1 gene in lipogenic tissues of domestic goats: a potential strategy for meat quality improvement.
Acetyl CoA Carboxylase 1 (ACC1) is a biotin-dependent enzyme that catalyzes the carboxylation of Acetyl CoA to form Malonyl CoA, the key intermediate metabolite in fatty acid synthesis. In this study, the mRNA expression of the ACC1 gene was evaluated in four different tissues (liver, visceral fat, subcutaneous fat, and longissimus muscle) of the domestic goat (Capra hircus) kids feeding on four different levels of trivalent chromium (0, 0.5, 1, and 1.5mg/day) as food supplementation. RT-qPCR technique was used for expression analyses and heat shock protein 90 gene (HSP-90) was considered as reference gene for data normalization. Our results revealed that 1.5mg/day chromium significantly reduced the expression of the ACC1 gene in liver, visceral fat, and subcutaneous fat tissues, but not in longissimus muscles (P<0.05). We measured some phenotypic traits of kid's carcasses to detect their probable correlations with chromium-mediated downregulation of ACC1 expression. Interestingly, changes in ACC1 expression were accompanied with decreased accumulation of fats in adipose tissues such that the subcutaneous fat thickness and heart fat percentage decreased in kids feeding on chromium. By contrast, chromium supplemented kids showed higher percentage of muscles despite the fact that their total body weight did not differ from that of non-supplemented kids. Our study suggests that trivalent chromium alters the direction of energy accumulation towards muscles rather than fats and provides insights into application of chromium supplementation as a useful strategy for improvement of meat quality in domestic animals.
Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran. Electronic address: mjnajafpanah@ut.ac.ir.
Mohammad Javad
MJ
Najafpanah
Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran. Electronic address: sadeghimos@ut.ac.ir.
Mostafa
M
Sadeghi
Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.
Abolfazl
A
Zali
Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.
Hossein
H
Moradi-Shahrebabak
Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.
Hojatollah
H
Mousapour
D000103:Acetyl-CoA Carboxylase / Q000235:genetics*; D000818:Animals; D002857:Chromium / Q000008:administration & dosage*; D019587:Dietary Supplements; D015536:Down-Regulation; D006041:Goats / Q000235:genetics*; D050152:Intra-Abdominal Fat / Q000201:enzymology; D050155:Lipogenesis / Q000235:genetics; D008099:Liver / Q000201:enzymology; D008297:Male; D008460:Meat; D018482:Muscle, Skeletal / Q000201:enzymology; D058996:Quality Improvement; D050151:Subcutaneous Fat / Q000201:enzymology
D002857:Chromium; D000103:Acetyl-CoA Carboxylase
Adipose tissue; Chromium; Downregulation; Expression of ACC1; Meat quality