Prognostic significance of specific anti-WT1 IgG antibody level in plasma in patients with ovarian carcinoma.
Ovarian carcinoma (OC) has a poor prognosis and lack early effective screening markers. Wilm's tumor gene 1 (WT1) is overexpressed in OCs. Therefore, it is of great interest to investigate whether WT1-specific antibody (Ab) measurements in plasma can serve as a biomarker of anti-OC response, and is of importance in relation to patient prognosis. Peripheral blood samples were obtained from a total of 103 women with ovarian tumors with median being 1 day (range 0-48 days) before operation. WT1 IgG Ab levels were evaluated using enzyme-linked immunosorbent assay (ELISA). Immunohistochemical analysis of WT1 protein expression was performed on OC tissue samples. We found that low-WT1 Ab level in plasma was related to improved survival in patients diagnosed at stages III-IV and grade 3 carcinomas. Positive WT1 protein staining on OC tissue samples had a negative impact on survival in the entire cohort, both overall survival (OS) (P = 0.046) and progression-free survival (PFS) (P = 0.006), but not in the serous OC subtype. Combining WT1 IgG Ab levels and WT1 staining, patients with high-WT1 IgG Ab levels in plasma and positive WT1 protein staining in cancer tissues had shorter survival, with a significant association in PFS (P = 0.016). These results indicated that WT1 Ab measurements in plasma and WT1 staining in tissue specimens could be useful as biomarkers for patient outcome in the high-risk subtypes of OCs for postoperative individualized therapy.
Clinical Chemistry, Department of Medical Biosciences, Umeå University, Umeå, Sweden; Pathology, Department of Medical Biosciences, Umeå University, Umeå, Sweden.
Charlotta
C
Andersson
Yusuke
Y
Oji
Nina
N
Ohlson
Sihan
S
Wang
Xingru
X
Li
Ulrika
U
Ottander
Eva
E
Lundin
Haruo
H
Sugiyama
Aihong
A
Li
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000912:Antibodies, Neoplasm / Q000097:blood*; D014408:Biomarkers, Tumor / Q000097:blood*; D018572:Disease-Free Survival; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D053208:Kaplan-Meier Estimate; D008875:Middle Aged; D018297:Neoplasms, Cystic, Mucinous, and Serous / Q000097:blood* / Q000175:diagnosis / Q000401:mortality; D010051:Ovarian Neoplasms / Q000097:blood* / Q000175:diagnosis / Q000401:mortality; D011379:Prognosis; D025721:WT1 Proteins / Q000276:immunology*; D055815:Young Adult
D000912:Antibodies, Neoplasm; D014408:Biomarkers, Tumor; D007074:Immunoglobulin G; D025721:WT1 Proteins; C583751:WT1 protein, human
Ovarian carcinoma; WT1; WT1 IgG antibody; prognosis