ROCK-2 is associated with focal adhesion maturation during myoblast migration.
Satellite cell migration is critical for skeletal muscle growth and regeneration. Controlled cell migration is dependent on the formation of mature focal adhesions between the cell and the underlying extracellular matrix (ECM). These cell-ECM interactions trigger the activation of signalling events such as the Rho/ROCK pathway. We have previously identified a specific role for ROCK-2 during myoblast migration. In this study we report that ROCK inhibition with Y-27632 increases C2C12 myoblast velocity, but at the expense of directional migration. In response to Y-27632 an increased number of smaller focal adhesions were distributed across adhesion sites that in turn were clearly larger than sites in untreated cells, suggesting a reduction in focal adhesion maturation. We also confirm ROCK-2 localisation to the focal adhesion sites in migrating myoblasts and demonstrate a change in the distribution of these ROCK-2 containing adhesions in response to Y-27632. Taken together, our observations provide further proof that ROCK-2 regulates directional myoblast migration through focal adhesion formation and maturation.
Discipline of Biochemistry, School of Life Sciences, University of KwaZulu-Natal, Pietermaritzburg, South Africa.
K P
KP
Goetsch
C
C
Snyman
K H
KH
Myburgh
C U
CU
Niesler
D000577:Amides / Q000494:pharmacology; D000818:Animals; D002448:Cell Adhesion; D002460:Cell Line; D002465:Cell Movement / Q000502:physiology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005109:Extracellular Matrix; D022001:Focal Adhesions / Q000502:physiology*; D051379:Mice; D024510:Muscle Development; D018482:Muscle, Skeletal / Q000254:growth & development; D032446:Myoblasts / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D011725:Pyridines / Q000494:pharmacology; D016596:Vinculin / Q000096:biosynthesis; D054460:rho-Associated Kinases / Q000037:antagonists & inhibitors*
D000577:Amides; D004791:Enzyme Inhibitors; D011725:Pyridines; D016596:Vinculin; C108830:Y 27632; C515855:Rock2 protein, mouse; D054460:rho-Associated Kinases
FOCAL ADHESIONS; MYOBLAST MIGRATION; Rho-KINASE\ROCK; WOUND REPAIR