Gene silencing of NOB1 by lentivirus suppresses growth and migration of human osteosarcoma cells.
NIN1/RPN12 binding protein 1 homolog (Saccharomyces cerevisiae) (NOB1) encodes a chaperone protein that joins the 20S proteasome with the 19S regulatory particle in the nucleus and facilitates the biogenesis of the 26S proteasome, which plays a role in maintaining cellular homeostasis by controlling protein degradation. In order to investigate the role of NOB1 in osteosarcoma, NOB1 protein expression in human osteosarcoma cell lines was assessed using western blot analysis. Lentivirus-mediated short hairpin RNA was employed to knock down NOB1, and the effects of NOB1 silencing on cell growth were assessed using MTT, colony formation and cell cycle assays. Cell migration was observed using the Transwell assay. In addition, the expression levels of E-cadherin and β-catenin were examined by western blot analysis. Functional analysis indicated that NOB1-knockdown markedly inhibited cell growth and caused G2/M-phase arrest in human osteosarcoma cells. Furthermore, NOB1 inhibition decreased cell migration and increased E-cadherin and β-catenin expression in U2OS cells. In conclusion, the present study suggested that NOB1 depletion may inhibit osteosarcoma development by increasing E-cadherin and β-catenin expression and, for the first time, indicated the potential of NOB1 as a target in osteosarcoma treatment.
Department of Orthopedics, The Second Hospital of Jilin University, Changchun, Jilin 130041, P.R. China.
Bingpeng
B
Chen
Department of Oncology, Jilin Tumor Hospital, Changchun, Jilin 130021, P.R. China.
Jingjing
J
Liu
Department of Orthopedics, The Second Hospital of Jilin University, Changchun, Jilin 130041, P.R. China.
Dankai
D
Wu
Department of Orthopedics, The Second Hospital of Jilin University, Changchun, Jilin 130041, P.R. China.
Yanguo
Y
Qin
Department of Orthopedics, The Second Hospital of Jilin University, Changchun, Jilin 130041, P.R. China.
Chuangang
C
Peng
Department of Orthopedics, The Second Hospital of Jilin University, Changchun, Jilin 130041, P.R. China.
Chen
C
Li
Department of Orthopedics, The Second Hospital of Jilin University, Changchun, Jilin 130041, P.R. China.
Jincheng
J
Wang
D001859:Bone Neoplasms / Q000235:genetics / Q000473:pathology; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation; D055785:Gene Knockdown Techniques; D020868:Gene Silencing; D005822:Genetic Vectors / Q000235:genetics*; D006801:Humans; D016086:Lentivirus / Q000235:genetics*; D009687:Nuclear Proteins / Q000235:genetics*; D012516:Osteosarcoma / Q000235:genetics / Q000473:pathology; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D016601:RNA-Binding Proteins / Q000235:genetics*
C506664:NOB1 protein, human; D009687:Nuclear Proteins; D034741:RNA, Small Interfering; D016601:RNA-Binding Proteins