Ruminal Prevotella spp. may play an important role in the conversion of plant lignans into human health beneficial antioxidants.
Secoisolariciresinol diglucoside (SDG), the most abundant lignan in flaxseed, is metabolized by the ruminal microbiota into enterolignans, which are strong antioxidants. Enterolactone (EL), the main mammalian enterolignan produced in the rumen, is transferred into physiological fluids, with potentially human health benefits with respect to menopausal symptoms, hormone-dependent cancers, cardiovascular diseases, osteoporosis and diabetes. However, no information exists to our knowledge on bacterial taxa that play a role in converting plant lignans into EL in ruminants. In order to investigate this, eight rumen cannulated cows were used in a double 4 × 4 Latin square design and fed with four treatments: control with no flax meal (FM), or 5%, 10% and 15% FM (on a dry matter basis). Concentration of EL in the rumen increased linearly with increasing FM inclusion. Total rumen bacterial 16S rRNA concentration obtained using Q-PCR did not differ among treatments. PCR-T-RFLP based dendrograms revealed no global clustering based on diet indicating between animal variation. PCR-DGGE showed a clustering by diet effect within four cows that had similar basal ruminal microbiota. DNA extracted from bands present following feeding 15% FM and absent with no FM supplementation were sequenced and it showed that many genera, in particular Prevotella spp., contributed to the metabolism of lignans. A subsequent in vitro study using selected pure cultures of ruminal bacteria incubated with SDG indicated that 11 ruminal bacteria were able to convert SDG into secoisolariciresinol (SECO), with Prevotella spp. being the main converters. These data suggest that Prevotella spp. is one genus playing an important role in the conversion of plant lignans to human health beneficial antioxidants in the rumen.
Institute of Biological, Environmental, and Rural Sciences (IBERS), Aberystwyth University, Aberystwyth, United Kingdom.
Ana L B
AL
Schogor
Institute of Biological, Environmental, and Rural Sciences (IBERS), Aberystwyth University, Aberystwyth, United Kingdom.
Sharon A
SA
Huws
Departamento de Zootecnia, Universidade Estadual de Maringá, Maringá, PR, Brazil.
Geraldo T D
GT
Santos
Institute of Biological, Environmental, and Rural Sciences (IBERS), Aberystwyth University, Aberystwyth, United Kingdom.
Nigel D
ND
Scollan
Institute of Biological, Environmental, and Rural Sciences (IBERS), Aberystwyth University, Aberystwyth, United Kingdom.
Barbara D
BD
Hauck
Institute of Biological, Environmental, and Rural Sciences (IBERS), Aberystwyth University, Aberystwyth, United Kingdom.
Ana L
AL
Winters
Department of Animal Science, Kyungpook National University, Sangju, Korea.
Eun J
EJ
Kim
Dairy and Swine Research and Development Centre, Agriculture and Agri-Food Canada, Sherbrooke, QC, Canada.
Hélène V
HV
Petit
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002417:Cattle; D005260:Female; D006801:Humans; D017705:Lignans / Q000378:metabolism*; D012150:Polymorphism, Restriction Fragment Length; D018720:Prevotella / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D013279:Stomach, Ruminant / Q000382:microbiology*
D000975:Antioxidants; D017705:Lignans; D012336:RNA, Ribosomal, 16S