Transmission of white spot syndrome virus (WSSV) from Dendronereis spp. (Peters) (Nereididae) to penaeid shrimp.
Dendronereis spp. (Peters) (Nereididae) is a common polychaete in shrimp ponds built on intertidal land and is natural food for shrimp in traditionally managed ponds in Indonesia. White spot syndrome virus (WSSV), an important viral pathogen of the shrimp, can replicate in this polychaete (Desrina et al. 2013); therefore, it is a potential propagative vector for virus transmission. The major aim of this study was to determine whether WSSV can be transmitted from naturally infected Dendronereis spp. to specific pathogen-free (SPF) Pacific white shrimp Litopenaeus vannamei (Boone) through feeding. WSSV was detected in naturally infected Dendronereis spp. and Penaeus monodon Fabricius from a traditional shrimp pond, and the positive animals were used in the current experiment. WSSV-infected Dendronereis spp. and P. monodon in a pond had a point prevalence of 90% and 80%, respectively, as measured by PCR. WSSV was detected in the head, gills, blood and mid-body of Dendronereis spp. WSSV from naturally infected Dendronereis spp was transmitted to SPF L. vannamei and subsequently from this shrimp to new naïve-SPF L. vannamei to cause transient infection. Our findings support the contention that Dendronereis spp, upon feeding, can be a source of WSSV infection of shrimp in ponds.
Faculty of Fisheries and Marine Sciences, Department of Fisheries, Diponegoro University, Semarang, Indonesia; Aquaculture and Fisheries Group, Wageningen University, Wageningen, The Netherlands.
D
D
Haryadi
J A J
JA
Verreth
M C J
MC
Verdegem
J M
JM
Vlak
D000818:Animals; D017756:Aquaculture; D033561:Penaeidae / Q000821:virology*; D011077:Polychaeta / Q000821:virology*; D013047:Specific Pathogen-Free Organisms; D046848:White spot syndrome virus 1 / Q000302:isolation & purification / Q000502:physiology*
Dendronereis spp.; infection; oral transmission; shrimp; white spot syndrome virus