Rapid identification of antioxidant compounds of Genista saharae Coss. & Dur. by combination of DPPH scavenging assay and HPTLC-MS.
Genista species are sources of antioxidant phenolic compounds such as O- and C-glycosylﬂavonoids and isoflavonoids. A combination of a DPPH scavenging assay with HPTLC-MS, a fast and efficient method for identification of bioactive compounds, has been applied for evaluation of the radical scavenging activity of metabolites from Genista saharae Coss. &amp; Dur. Different organs collected at various periods have been compared. Identification of antioxidant compounds was obtained by elution of the major DPPH-inhibition zones. The resulting HPTLC-MS analysis under moderately polar conditions, coupled to the DPPH results led to the putative identification of two antioxidant isoflavone aglycones: 3',4',5,7-tetrahydroxyisoflavone (1) and ficuisoflavone (3), whereas polar migration conditions led to the identification of the glycosides 5-methoxy-4',7-trihydroxy-8-glucopyranosylisoflavone (4) and 4',5-dihydroxy-7-methoxyisoflavone-4'-O-β-D-gluco-pyranoside (5). Evaluation of percentage of inhibition of DPPH radical by the purified isoflavone 4 from the root extract showed that it affords a moderate contribution to the total radical scavenging activity of the extract.
Laboratoire de Phytothérapie Appliquée aux Maladies Chroniques, Université Ferhat Abbas, Sétif 19000, Algeria. merianed@yahoo.fr.
Djamila
D
Meriane
Laboratoire de Pharmacognosie, U.M.R./C.N.R.S. 8638, Faculté des Sciences Pharmaceutiques et Biologiques, Université Paris Descartes, Sorbonne Paris Cité, 4 Avenue de l'Observatoire, F-75006 Paris, France. gregory.genta-jouve@parisdescartes.fr.
Grégory
G
Genta-Jouve
Laboratoire de Phytothérapie Appliquée aux Maladies Chroniques, Université Ferhat Abbas, Sétif 19000, Algeria. Labo_brpdz19@hotmail.com.
Mohamed
M
Kaabeche
Laboratoire de Pharmacognosie, U.M.R./C.N.R.S. 8638, Faculté des Sciences Pharmaceutiques et Biologiques, Université Paris Descartes, Sorbonne Paris Cité, 4 Avenue de l'Observatoire, F-75006 Paris, France. sylvie.michel@parisdescartes.fr.
Sylvie
S
Michel
Laboratoire de Pharmacognosie, U.M.R./C.N.R.S. 8638, Faculté des Sciences Pharmaceutiques et Biologiques, Université Paris Descartes, Sorbonne Paris Cité, 4 Avenue de l'Observatoire, F-75006 Paris, France. sabrina.boutefnouchet@parisdescartes.fr.
Sabrina
S
Boutefnouchet
D001713:Biphenyl Compounds / Q000037:antagonists & inhibitors*; D002855:Chromatography, Thin Layer / Q000379:methods; D016166:Free Radical Scavengers / Q000737:chemistry* / Q000302:isolation & purification; D039761:Genista / Q000737:chemistry*; D006027:Glycosides / Q000737:chemistry* / Q000302:isolation & purification; D007529:Isoflavones / Q000737:chemistry* / Q000302:isolation & purification; D013058:Mass Spectrometry; D010851:Picrates / Q000037:antagonists & inhibitors*; D010936:Plant Extracts / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry
D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D006027:Glycosides; D007529:Isoflavones; D010851:Picrates; D010936:Plant Extracts; C004931:1,1-diphenyl-2-picrylhydrazyl