Metabolic adaptations in skeletal muscle, adipose tissue, and whole-body oxidative capacity in response to resistance training.
OBJECTIVE
The effects of resistance training on mitochondrial biogenesis and oxidative capacity in skeletal muscle are not fully characterized, and even less is known about alterations in adipose tissue. We aimed to investigate adaptations in oxidative metabolism in skeletal muscle and adipose tissue after 8 weeks of heavy resistance training in apparently healthy young men.


METHODS
Expression of genes linked to oxidative metabolism in the skeletal muscle and adipose tissue was assessed before and after the training program. Body composition, peak oxygen uptake (VO2 peak), fat oxidation, activity of mitochondrial enzyme in muscle, and serum adiponectin levels were also determined before and after resistance training.


RESULTS
In muscle, the expression of the genes AdipoR1 and COX4 increased after resistance training (9 and 13 %, respectively), whereas the expression levels of the genes PGC-1α, SIRT1, TFAM, CPT1b, and FNDC5 did not change. In adipose tissue, the expression of the genes SIRT1 and CPT1b decreased after training (20 and 23 %, respectively). There was an increase in lean mass (from 59.7 ± 6.1 to 61.9 ± 6.2 kg), VO2 peak (from 49.7 ± 5.5 to 56.3 ± 5.0 ml/kg/min), and fat oxidation (from 6.8 ± 2.1 to 9.1 ± 2.7 mg/kg fat-free mass/min) after training, whereas serum adiponectin levels decreased significantly and enzyme activity of citrate synthase and 3-hydroxyacyl-CoA dehydrogenase did not change.


CONCLUSIONS
Despite significant increases in VO2 peak, fat oxidation, and lean mass following resistance training, the total effect on gene expression and enzyme activity linked to oxidative metabolism was moderate.
Department of Public Health and Clinical Medicine, Medicine, Umeå University, Umeå, Sweden, malin.alvehus@medicin.umu.se.
Malin
M
Alvehus
Niklas
N
Boman
Karin
K
Söderlund
Michael B
MB
Svensson
Jonas
J
Burén
D000222:Adaptation, Physiological; D000273:Adipose Tissue / Q000378:metabolism*; D000328:Adult; D015415:Biomarkers / Q000097:blood; D001823:Body Composition; D004734:Energy Metabolism / Q000235:genetics; D005786:Gene Expression Regulation; D064368:Healthy Volunteers; D006801:Humans; D008297:Male; D008931:Mitochondria, Muscle / Q000378:metabolism; D018482:Muscle, Skeletal / Q000378:metabolism*; D010084:Oxidation-Reduction; D010101:Oxygen Consumption; D055070:Resistance Training; D013997:Time Factors; D055815:Young Adult
D015415:Biomarkers