Immunohistochemical analysis of matrix metalloproteinase-13 in human caries dentin.
The immunoexpression profile of matrix metalloproteinase-13 was investigated for the first time in dentin of human caries and healthy teeth. Twelve permanent premolars (10 caries and 2 sound) were decalcified in ethylenediaminetetraacetic acid and processed for embedding in paraffin wax. Sections 3-4 µm in thickness were cut and processed for immunohistochemistry. A mouse monoclonal anti-metalloproteinase-13 antibody was used for localisation using an immunoperoxidase technique. Dentinal immunoreactivity was detected in all teeth; it was weak in sound teeth and strong close to the caries area. These in vivo findings suggest a role for metalloproteinase-13 in the development and progression of adult human dental tissue disorders.
University of Catania. carla.loreto@unict.it.
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Loreto
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Galanti
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Musumeci
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Rusu
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Leonardi
D000328:Adult; D000818:Animals; D001641:Bicuspid / Q000201:enzymology* / Q000473:pathology; D003731:Dental Caries / Q000201:enzymology* / Q000473:pathology; D003804:Dentin / Q000201:enzymology* / Q000473:pathology; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D008297:Male; D053509:Matrix Metalloproteinase 13 / Q000096:biosynthesis*; D051379:Mice
C507079:MMP13 protein, human; D053509:Matrix Metalloproteinase 13