Effect of 1-octyl-3-methylimidazolium chloride on cell replication and membrane permeability of Escherichia coli DH5α.
Ionic liquids (ILs) have elicited attention due to their unique properties. ILs may pose environmental risks to aquatic ecosystems once released into water during generation and application. Therefore, the toxic and antimicrobial properties of ILs should be analysed. This study aims to evaluate the cytotoxicity of 1-octyl-3-methylimidazolium chloride ([C8mim] [Cl]) on Escherichia coli DH5α by MTT (3-[4,5-dimethylthiazol-2yl]-2,5 diphenyl tetrazolium bromide) assay, and to determine the effect of [C8mim] [Cl] on the replication and membrane permeability of E. coli DH5α. The results showed that [C8mim] [Cl] decreased cell viability and inhibited bacterial cell replication. [C8mim] [Cl] increased protein and nucleic acid contents in the extracellular fluid, damaged the cell membrane, and increased membrane permeability. The increase of cell membrane permeability induced by [C8mim] [Cl] could be the cause of decreased cell viability and replication.
College of Life Sciences and Technology, Xinxiang Medical University, 601 Jinsui Road, Xinxiang, 453003, Henan, People's Republic of China, jingchangqin@126.com.
Changqin
C
Jing
Lingmin
L
Mu
Taifang
T
Ren
Bingnan
B
Li
Sujuan
S
Chen
Wenbin
W
Nan
D001881:Borates / Q000633:toxicity*; D002455:Cell Division / Q000187:drug effects*; D002462:Cell Membrane / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects*; D007093:Imidazoles / Q000633:toxicity*; D010539:Permeability / Q000187:drug effects; D014874:Water Pollutants, Chemical / Q000633:toxicity*
C528768:1-octyl-3-methylimidazolium; D001881:Borates; D007093:Imidazoles; D014874:Water Pollutants, Chemical