Protective effect of boric acid on lead- and cadmium-induced genotoxicity in V79 cells.
The toxic heavy metals cadmium (Cd) and lead (Pb) are important environmental pollutants which can cause serious damage to human health. As the metal ions (Cd(2+) and Pb(2+)) accumulate in the organism, there is special concern regarding chronic toxicity and damage to the genetic material. Metal-induced genotoxicity has been attributed to indirect mechanisms, such as induction of oxidative stress and interference with DNA repair. Boron is a naturally occurring element and considered to be an essential micronutrient, although the cellular activities of boron compounds remain largely unexplored. The present study has been conducted to evaluate potential protective effects of boric acid (BA) against genotoxicity induced by cadmium chloride (CdCl2) and lead chloride (PbCl2) in V79 cell cultures. Cytotoxicity assays (neutral red uptake and cell titer blue assay) served to determine suitable concentrations for subsequent genotoxicity assays. Chromosomal damage and DNA strand breaks were assessed by micronucleus tests and comet assays. Both PbCl2 and CdCl2 (at 3, 5 and 10 µM) were shown to induce concentration-dependent increases in micronucleus frequencies and DNA strand breaks in V79 cells. BA itself was not cytotoxic (up to 300 µM) and showed no genotoxic effects. Pretreatment of cells with low levels of BA (2.5 and 10 µM) was found to strongly reduce the genotoxic effects of the tested metals. Based on the findings of this in vitro study, it can be suggested that boron provides an efficient protection against the induction of DNA strand breaks and micronuclei by lead and cadmium. Further studies on the underlying mechanisms for the protective effect of boron are needed.
Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Ankara University, 06100, Tandogan, Ankara, Turkey, dur@pharmacy.ankara.edu.tr.
Aylin
A
Ustündağ
Claudia
C
Behm
Wolfram
W
Föllmann
Yalçin
Y
Duydu
Gisela H
GH
Degen
D000818:Animals; D001888:Boric Acids / Q000008:administration & dosage / Q000494:pharmacology*; D019256:Cadmium Chloride / Q000633:toxicity*; D002478:Cells, Cultured; D020552:Comet Assay; D006224:Cricetinae; D003412:Cricetulus; D004249:DNA Damage / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D000075322:Heavy Metal Poisoning; D007854:Lead / Q000633:toxicity*; D015162:Micronucleus Tests; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D011041:Poisoning / Q000517:prevention & control
D001888:Boric Acids; D009153:Mutagens; D007854:Lead; C029891:lead chloride; D019256:Cadmium Chloride; C032688:boric acid