[Wif-1 methylation and β-catenin expression in colorectal serrated lesions].
OBJECTIVE
To investigate methylation status of Wif-1 and β-catenin expression in colorectal serrated lesions.


METHODS
Various colorectal lesions were collected including 52 cases of hyperplastic polyps, 41 cases of sessile serrated adenoma, 23 cases of traditional serrated adenoma, 24 cases of colorectal cancer and 24 cases of normal mucosa. All specimens were subject to immunohistochemical staining of β-catenin.SYBR Green PCR analysis of Wif-1 promoter methylation was performed in 29 cases of hyperplastic polyps, 29 cases of sessile serrated adenoma, 19 cases of traditional serrated adenoma, 14 cases of colorectal cancer and 16 cases of normal mucosa.


RESULTS
Abnormal expression rates of β-catenin in normal mucosa, hyperplastic polyps, sessile serrated adenoma, traditional serrated adenoma and colorectal cancer were 12.5% (3/24), 59.6% (31/52), 63.4% (26/41), 73.9% (17/23) and 100.0% (24/24), respectively. The corresponding methylation rates of Wif-1 promoter were 2/16, 10/29 (34.5%), 16/29 (55.2%), 15/19 and 13/14 (P < 0.05), respectively. Abnormal β-catenin expression was positively correlated with Wif-1 promoter methylation in traditional serrated adenomas (r = 0.536, P < 0.05).


CONCLUSIONS
Abnormal β-catenin expression and methylation rate of Wif-1 promoter are significantly higher in colorectal serrated lesions. Methylation of Wif-1 promoter may be related to the abnormal expression of β-catenin through activation of Wnt/β-catenin signaling pathway, which may contribute to the development of colorectal serrated lesions.
Department of Pathology, General Hospital of Beijing Military Area, Beijing 100700, China.
Yuan
Y
Fang
Department of Pathology, General Hospital of Beijing Military Area, Beijing 100700, China. E-mail: bzwlp@yahoo.com.
Luping
L
Wang
Department of Pathology, General Hospital of Beijing Military Area, Beijing 100700, China.
Yuping
Y
Zhang
Department of Pathology, General Hospital of Beijing Military Area, Beijing 100700, China.
Chang
C
Ge
Department of Pathology, General Hospital of Beijing Military Area, Beijing 100700, China.
Chunwei
C
Xu
D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics* / Q000378:metabolism; D000236:Adenoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D002277:Carcinoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D015179:Colorectal Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D019175:DNA Methylation; D006801:Humans; D006965:Hyperplasia; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000378:metabolism; D007417:Intestinal Polyps / Q000473:pathology; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism; D060449:Wnt Signaling Pathway; D051176:beta Catenin / Q000378:metabolism*
D048868:Adaptor Proteins, Signal Transducing; C495270:CTNNB1 protein, human; D012097:Repressor Proteins; C485569:WIF1 protein, human; D051176:beta Catenin