Association of human papilloma virus with atypical and malignant oral papillary lesions.
OBJECTIVE
This study aimed to examine atypical and malignant papillary oral lesions for low- and high-risk human papillomavirus (HPV) infection and to correlate HPV infection with clinical and pathologic features.


METHODS
Sections of 28 atypical papillary lesions (APLs) and 14 malignant papillary lesions (MPLs) were examined for HPV by in situ hybridization and for p16 and MIB-1 by immunohistochemistry; 24 conventional papillomas were studied for comparison.


RESULTS
Low-risk HPV was found in 10 of 66 cases, including 9 APLs and 1 papilloma. All low-risk HPV-positive cases showed suprabasilar MIB-1 staining, and the agreement was statistically significant (P < .0001). Diffuse p16 staining combined with high-risk HPV was not seen in any of the cases. A subset of HPV(-) APLs progressed to carcinoma.


CONCLUSIONS
Oral papillary lesions are a heterogeneous group. Low-risk HPV infection is associated with a subset of APLs with a benign clinical course. Potentially malignant APLs and MPLs are not associated with low- or high-risk HPV.
Faculty of Dentistry, University of Toronto, Toronto, Ontario, Canada. Electronic address: Christina.mccord@utoronto.ca.
Christina
C
McCord
Applied Molecular Profiling Laboratory, Ontario Cancer Institute, University of Toronto, Toronto, Ontario, Canada.
Jing
J
Xu
Department of Biostatistics, University Health Network, Toronto, Ontario, Canada.
Wei
W
Xu
Department of Biostatistics, University Health Network, Toronto, Ontario, Canada.
Xin
X
Qiu
Department of Surgical Oncology/Otolaryngology-Head and Neck Surgery, Princess Margaret Cancer Centre, University of Toronto, Toronto, Ontario, Canada.
Nidal
N
Muhanna
Department of Surgical Oncology/Otolaryngology-Head and Neck Surgery, Princess Margaret Cancer Centre, University of Toronto, Toronto, Ontario, Canada.
Jonathan
J
Irish
Faculty of Dentistry, University of Toronto, Toronto, Ontario, Canada; Department of Pathology and Laboratory Medicine, Mount Sinai Hospital, Toronto, Ontario, Canada.
Iona
I
Leong
Faculty of Dentistry, University of Toronto, Toronto, Ontario, Canada.
Richard John
RJ
McComb
Department of Pathology, University Health Network, Toronto, Ontario, Canada.
Bayardo
B
Perez-Ordonez
Faculty of Dentistry, University of Toronto, Toronto, Ontario, Canada; Ontario Cancer Institute, University Health Network, University of Toronto, Toronto, Ontario, Canada.
Grace
G
Bradley
D000368:Aged; D000974:Antibodies, Antinuclear / Q000494:pharmacology; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D014408:Biomarkers, Tumor / Q000378:metabolism; D001706:Biopsy; D004279:DNA, Viral / Q000378:metabolism; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D019394:Ki-67 Antigen / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000821:virology*; D010212:Papilloma / Q000821:virology*; D011230:Precancerous Conditions / Q000821:virology; D012189:Retrospective Studies; D012307:Risk Factors
D000974:Antibodies, Antinuclear; D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D004279:DNA, Viral; D019394:Ki-67 Antigen; C440408:MIB-1 antibody