Structure and properties of a (1→3)-β-D-glucan from ultrasound-degraded exopolysaccharides of a medicinal fungus.
A high molecular weight (MW) exopolysaccaride (EPS) fraction EPS1 was isolated from the fermentation broth of a medicinal fungus Cordyceps sinensis Cs-HK1 and partially degraded by high-intensity ultrasound (US) into a lower MW fraction EPS1U. EPS1U exhibited a single, symmetric peak on size exclusion chromatography with an average MW of 730kDa by light scattering analysis. It had a much lower intrinsic viscosity (1.7 versus 15.6dL/g) but a much higher solubility in water (77.5 versus 5.1g/L) than EPS1. Based on methylation analysis and NMR spectrometry, the structure of EPS1U was deduced as a (1→3)-β-d-glucan with glucose side chains attached to O-6 position at the branching points. EPS1U showed a high moisture absorption capability comparable to chitosan and urea, suggesting its potential as a moisturizing agent for food and cosmeceutical application. This is the first report on a high MW (1→3)-β-d-glucan isolated from EPS produced by Cordyceps sinensis.
Department of Applied Biology & Chemical Technology, State Key Laboratory of Chinese Medicine and Molecular Pharmacology in Shenzhen, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong.
Xia
X
Chen
Department of Applied Biology & Chemical Technology, State Key Laboratory of Chinese Medicine and Molecular Pharmacology in Shenzhen, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong.
Ka-Chai
KC
Siu
Department of Applied Biology & Chemical Technology, State Key Laboratory of Chinese Medicine and Molecular Pharmacology in Shenzhen, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong.
Yi-Ching
YC
Cheung
Department of Applied Biology & Chemical Technology, State Key Laboratory of Chinese Medicine and Molecular Pharmacology in Shenzhen, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong. Electronic address: jian-yong.wu@polyu.edu.hk.
Jian-Yong
JY
Wu
D065966:Absorption, Physicochemical; D002236:Carbohydrate Conformation; D048271:Chitosan / Q000737:chemistry; D002850:Chromatography, Gel; D032661:Cordyceps / Q000737:chemistry*; D005285:Fermentation; D008745:Methylation; D008970:Molecular Weight; D025282:Mycelium / Q000737:chemistry; D019906:Nuclear Magnetic Resonance, Biomolecular; D011134:Polysaccharides / Q000737:chemistry*; D012995:Solubility; D013010:Sonication; D014508:Urea / Q000737:chemistry; D014783:Viscosity; D047071:beta-Glucans / Q000737:chemistry / Q000302:isolation & purification*
D011134:Polysaccharides; D047071:beta-Glucans; D014508:Urea; D048271:Chitosan
(1→3)-β-d-glucan; Cordyceps sinensis; Exopolysaccharide; Moisture absorption; Mycelial fermentation; Ultrasonic degradation