Cellular uptake of Nigella sativa oil-PLGA microparticle by PC-12 cell line.
The aim of this study is to investigate the cell uptake of Nigella sativa oil (NSO)-PLGA microparticle by neuron-like PC-12 cells in comparison to surfactants; hydrophilic (Tween 80 & Triton X100) and hydrophobic (Span 80). Solvent evaporation was used to precisely control the size, zeta potential and morphology of the particle. The results revealed varying efficiencies of the cell uptake by PC-12 cells, which may be partially attributed to the surface hydrophobicity of the microparticles. Interestingly, the uptake efficiency of PC-12 cells was higher with the more hydrophilic microparticle. NSO microparticle showed evidence of being preferably internalised by mitotic cells. Tween 80 microparticle showed the highest cell uptake efficiency with a concentration-dependent pattern suggesting its use as uptake enhancer for non-scavenging cells. In conclusion, PC-12 cells can take up NSO-PLGA microparticle which may have potential in the treatment of neurodegenerative disease.
Department of Pharmaceutical Technology, Faculty of Pharmacy, International Islamic University Malaysia (IIUM) , Kuantan , Malaysia .
Abd Almonem
AA
Doolaanea
Nur 'Izzati
N'
Mansor
Nurul Hafizah
NH
Mohd Nor
Farahidah
F
Mohamed
D000818:Animals; D006601:Hexoses / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D019344:Lactic Acid; D019636:Neurodegenerative Diseases / Q000188:drug therapy*; D017830:Octoxynol / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D016716:PC12 Cells; D010938:Plant Oils / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011136:Polysorbates / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D051381:Rats; D013501:Surface-Active Agents / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D029974:Carum
D006601:Hexoses; D010938:Plant Oils; D011136:Polysorbates; D013501:Surface-Active Agents; C018665:sorbitan monooleate; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D017830:Octoxynol; C104525:caraway oil
Hydrophobicity; Nigella sativa oil; PLGA; microparticles; neurodegenerative; surfactant; uptake