Characterization and antioxidant activities of degraded polysaccharides from Poria cocos sclerotium.
Poria cocos F.A.Wolf is a Chinese traditional medicine used to treat chronic gastritis, edema, nephrosis, gastric atony, and acute gastroenteric catarrh. Polysaccharides are the main active component of P. cocos. We obtained polysaccharides PCP-1, PCP-2, and PCP-3 from the degradation of P. cocos polysaccharides (PCP) with different concentrations of H2O2 solution. Molecular weights were determined by high performance size exclusion chromatography. HPLC analysis of monosaccharide composition confirmed that PCP-1, PCP-2, and PCP-3 are heteropolysaccharides composed of glucose and arabinose. IR spectra indicated obvious characteristic peaks of polysaccharides. The antioxidant activities of these polysaccharides were evaluated by established in vitro systems, including scavenging activity of hydroxyl radicals, ABTS radicals, and ferrous ions. The degradation polysaccharides exhibited obvious and concentration-dependent antioxidant properties. In addition, DNA binding analysis showed that PCP-1 had a stronger capacity than other polysaccharides to interact with DNA. However, each polysaccharide had a certain capacity for DNA damage protection.
School of Biology & Pharmacy Engineering, Wuhan Polytechnic University, 430023 Wuhan, China.
Jin
J
Tang
Hubei Institute for Food and Drug Control, 430023 Wuhan, China.
Jing
J
Nie
Hubei Institute for Food and Drug Control, 430023 Wuhan, China.
Danping
D
Li
School of Biology & Pharmacy Engineering, Wuhan Polytechnic University, 430023 Wuhan, China.
Wenjun
W
Zhu
School of Biology & Pharmacy Engineering, Wuhan Polytechnic University, 430023 Wuhan, China.
Shaopeng
S
Zhang
School of Biology & Pharmacy Engineering, Wuhan Polytechnic University, 430023 Wuhan, China.
Fang
F
Ma
School of Biology & Pharmacy Engineering, Wuhan Polytechnic University, 430023 Wuhan, China.
Qiao
Q
Sun
School of Biology & Pharmacy Engineering, Wuhan Polytechnic University, 430023 Wuhan, China.
Jia
J
Song
School of Biology & Pharmacy Engineering, Wuhan Polytechnic University, 430023 Wuhan, China.
Yonglian
Y
Zheng
School of Biology & Pharmacy Engineering, Wuhan Polytechnic University, 430023 Wuhan, China. Electronic address: chenpingvip24@163.com.
Ping
P
Chen
D000975:Antioxidants / Q000302:isolation & purification* / Q000378:metabolism* / Q000494:pharmacology; D004249:DNA Damage / Q000187:drug effects / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D016166:Free Radical Scavengers / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D011134:Polysaccharides / Q000302:isolation & purification* / Q000378:metabolism* / Q000494:pharmacology; D055441:Poria
D000975:Antioxidants; D016166:Free Radical Scavengers; D011134:Polysaccharides
Antioxidant activity; Degradation; Polysaccharide; Poria cocos F.A.Wolf