Crystallization and preliminary X-ray analysis of a novel type of lipolytic hydrolase from Bacillus licheniformis.
With increasing demand in biotechnological applications, the identification and characterization of novel lipolytic enzymes are of great importance. The crystallization and preliminary X-ray crystallographic study of a novel type of hydrolase from Bacillus licheniformis (BL28) are described here. Recombinant BL28 protein containing a C-terminal His tag was overproduced in Escherichia coli and purified to homogeneity. BL28 was crystallized using 0.2 M ammonium acetate, 0.1 M sodium citrate tribasic dihydrate pH 5.6, 30%(w/v) PEG 4000 as a crystallizing solution. X-ray diffraction data were collected to a resolution of 1.67 Å with an Rmerge of 5.8%. The BL28 crystals belonged to the tetragonal space group P41212, with unit-cell parameters a = b = 57.89, c = 167.25 Å. A molecular-replacement solution was obtained and structure refinement of BL28 is in progress.
Department of Applied Chemistry and Biological Engineering, College of Engineering, Ajou University, Suwon 443-749, Republic of Korea.
Hansol
H
Ju
Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon 440-746, Republic of Korea.
Ramesh
R
Pandian
Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon 440-746, Republic of Korea.
Kyungmin
K
Kim
Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon 440-746, Republic of Korea.
Kyeong Kyu
KK
Kim
Department of Applied Chemistry and Biological Engineering, College of Engineering, Ajou University, Suwon 443-749, Republic of Korea.
T Doohun
TD
Kim
D001407:Bacillus / Q000201:enzymology*; D003460:Crystallization / Q000379:methods*; D018360:Crystallography, X-Ray / Q000379:methods*; D006867:Hydrolases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008066:Lipolysis; D011994:Recombinant Proteins / Q000737:chemistry* / Q000235:genetics
D011994:Recombinant Proteins; D006867:Hydrolases
Bacillus licheniformis; lipolytic hydrolase