Wound healing efficacy of a chitosan-based film-forming gel containing tyrothricin in various rat wound models.
The objective of this study was to evaluate the healing effects of a chitosan-based, film-forming gel containing tyrothricin (TYR) in various rat wound models, including burn, abrasion, incision, and excision models. After solidification, the chitosan film layer successfully covered and protected a variety of wounds. Wound size was measured at predetermined timepoints after wound induction, and the effects of the film-forming gel were compared with negative (no treatment) and positive control groups (commercially available sodium fusidate ointment and TYR gel). In burn, abrasion and excision wound models, the film-forming gel enabled significantly better healing from 1 to 6 days after wound induction, compared with the negative control. Importantly, the film-forming gel also enabled significantly better healing compared with the positive control treatments. In the incision wound model, the breaking strength of wound strips from the group treated with the film-forming gel was significantly increased compared with both the negative and positive control groups. Histological studies revealed advanced granulation tissue formation and epithelialization in wounds treated with the film-forming gel. We hypothesize that the superior healing effects of the film-forming gel are due to wound occlusion, conferred by the chitosan film. Our data suggest that this film-forming gel may be useful in treating various wounds, including burn, abrasion, incision and excision wounds.
School of Pharmacy, Sungkyunkwan University, Jangan-gu, Suwon, Gyeonggi-do, 440-746, Republic of Korea.
Ju-Young
JY
Kim
Joon-Ho
JH
Jun
Sang-Joon
SJ
Kim
Kyu-Mok
KM
Hwang
Sung Rak
SR
Choi
Sang Duk
SD
Han
Mi-Won
MW
Son
Eun-Seok
ES
Park
D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D048271:Chitosan / Q000737:chemistry*; D004195:Disease Models, Animal; D004337:Drug Carriers / Q000737:chemistry*; D005260:Female; D005782:Gels; D015394:Molecular Structure; D017207:Rats, Sprague-Dawley; D014449:Tyrothricin / Q000008:administration & dosage / Q000627:therapeutic use*; D014945:Wound Healing / Q000187:drug effects*; D014947:Wounds and Injuries / Q000188:drug therapy*
D000900:Anti-Bacterial Agents; D004337:Drug Carriers; D005782:Gels; D014449:Tyrothricin; D048271:Chitosan