Modern techniques for the analysis of chromatin and nuclear organization in C. elegans.
In recent years, Caenorhabditis elegans has emerged as a new model to investigate the relationships between nuclear architecture, cellular differentiation, and organismal development. On one hand, C. elegans with its fixed lineage and transparent body is a great model organism to observe gene functions in vivo in specific cell types using microscopy. On the other hand, two different techniques have been applied in nematodes to identify binding sites for chromatin-associated proteins genome-wide: chromatin immunoprecipitation (ChIP), and Dam-mediated identification (DamID). We summarize here all three techniques together as they are complementary. We also highlight strengths and differences of the individual approaches.
Andalusian Center for Developmental Biology (CABD), CSIC/JA/Universidad Pablo de Olavide, Seville, Spain. pask@upo.es.
Peter
P
Askjaer
Sevinç
S
Ercan
Peter
P
Meister
D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics*; D002843:Chromatin; D047369:Chromatin Immunoprecipitation; D018509:DNA, Helminth; D005821:Genetic Techniques; D020411:Oligonucleotide Array Sequence Analysis
D002843:Chromatin; D018509:DNA, Helminth