Enhancing exopolysaccharide antioxidant formation and yield from Phellinus species through medium optimization studies.
The study was conducted to enhance exopolysaccharide (EPS) antioxidant formation (as measured by Trolox-Equivalent Antioxidant Capacity, TEAC) by Phellinus sp. P0988 through medium studies, while jointly increasing EPS yield. Out of seven medium components, four significant variables were identified based on linear models with statistical significance (p<0.05), namely rutin, FeSO4, and aspartate, which influenced TEAC, and malt extract, which influenced yield. Based on regression analysis of the experimental results from central composite design in response surface models, two second-order polynomial models adequately representing the relationship between these variables and EPS TEAC and yield were obtained, and the optimum concentration of these variables were determined and validated. Variable combinations for high EPS TEAC and yield were optimized through a desirability function test. The maximum EPS TEAC and yield reached 8.49 ± 0.32 mM and 10.92 ± 0.68 g/L, respectively, in a 7 L bioreactor under the optimum variable combinations.
Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, Ministry of Education, National Engineering Laboratory for Resource Developing of Endangered Chinese Crude Drugs in Northwest of China, College of Life Sciences, Shaanxi Normal University, Xi'an 710062, Shaanxi, China. Electronic address: biomarkuis@gmail.com.
Xiao-Kui
XK
Ma
Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, Ministry of Education, National Engineering Laboratory for Resource Developing of Endangered Chinese Crude Drugs in Northwest of China, College of Life Sciences, Shaanxi Normal University, Xi'an 710062, Shaanxi, China. Electronic address: bioma2003@163.com.
Hong
H
Zhang
Department of Chemical Engineering, Queen's University, Canada. Electronic address: ecp.eric@gmail.com.
Eric Charles
EC
Peterson
Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, Ministry of Education, National Engineering Laboratory for Resource Developing of Endangered Chinese Crude Drugs in Northwest of China, College of Life Sciences, Shaanxi Normal University, Xi'an 710062, Shaanxi, China. Electronic address: markuis@163.com.
Ling
L
Chen
D000975:Antioxidants / Q000378:metabolism*; D001487:Basidiomycota / Q000378:metabolism*; D019149:Bioreactors / Q000382:microbiology; D017076:Computer-Aided Design; D011134:Polysaccharides / Q000096:biosynthesis*
D000975:Antioxidants; D011134:Polysaccharides
Bioprocess design; Biosynthesis; Exopolysaccharide (EPS); Fermentation; Modeling; Phellinus sp. P0988