Mitotic position and morphology of committed precursor cells in the zebrafish retina adapt to architectural changes upon tissue maturation.
The development of complex neuronal tissues like the vertebrate retina requires the tight orchestration of cell proliferation and differentiation. Although the complexity of transcription factors and signaling pathways involved in retinogenesis has been studied extensively, the influence of tissue maturation itself has not yet been systematically explored. Here, we present a quantitative analysis of mitotic events during zebrafish retinogenesis that reveals three types of committed neuronal precursors in addition to the previously known apical progenitors. The identified precursor types present at distinct developmental stages and exhibit different mitotic location (apical versus nonapical), cleavage plane orientation, and morphology. Interestingly, the emergence of nonapically dividing committed bipolar cell precursors can be linked to an increase in apical crowding caused by the developing photoreceptor cell layer. Furthermore, genetic interference with neuronal subset specification induces ectopic divisions of committed precursors, underlining the finding that progressing morphogenesis can effect precursor division position.
Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstraße 108, 01307 Dresden, Germany.
Isabell P
IP
Weber
Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstraße 108, 01307 Dresden, Germany.
Ana P
AP
Ramos
Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstraße 108, 01307 Dresden, Germany.
Paulina J
PJ
Strzyz
Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstraße 108, 01307 Dresden, Germany.
Louis C
LC
Leung
RIKEN Quantitative Biology Center, Osaka 565-0874, Japan; Department of Biological Sciences, Osaka University, Osaka 560-0043, Japan.
Stephen
S
Young
Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstraße 108, 01307 Dresden, Germany. Electronic address: norden@mpi-cbg.de.
Caren
C
Norden
D000222:Adaptation, Physiological; D000818:Animals; D019070:Cell Lineage; D008938:Mitosis; D058953:Neural Stem Cells / Q000166:cytology* / Q000378:metabolism / Q000502:physiology; D055495:Neurogenesis; D020419:Photoreceptor Cells, Vertebrate / Q000166:cytology* / Q000378:metabolism / Q000502:physiology; D012165:Retinal Ganglion Cells / Q000166:cytology* / Q000378:metabolism / Q000502:physiology; D015027:Zebrafish / Q000196:embryology* / Q000378:metabolism