Protective effects of apocynin nitrone on acute lung injury induced by lipopolysaccharide in rats.
Acute lung injury (ALI) is a major cause of mortality and morbidity worldwide. In a previous study we reported the synthesis of a series of apocynin derivatives. Although the anti-inflammatory activity of them contributes to these cytoprotective effects. However, the mechanisms and effects of improving LPS-induced ALI in vivo remain unknown, so the purpose of our investigation was designed to reveal the effect of apocynin derivatives on LPS-induced acute lung injury in rats. The present study showed that apocynin derivatives reduces overall protein levels and tumor necrosis factor α (TNF-α) level, inhibits the activation of NADPH oxidase, and increases the levels of superoxide dismutase (SOD). Especially, compound 11 significantly reduces pulmonary vascular permeability, white blood cell content and protein expressions of p67(phox) and p47(phox). These results suggest that compound 11 can ameliorate ALI induced by LPS through inhibition of NADPH oxidase.
Institute of New Drug Research, Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine, Jinan University College of Pharmacy, Guangzhou 510632, China.
Lipeng
L
Xu
Institute of New Drug Research, Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine, Jinan University College of Pharmacy, Guangzhou 510632, China.
Yongmei
Y
Li
Institute of New Drug Research, Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine, Jinan University College of Pharmacy, Guangzhou 510632, China.
Sainan
S
Wan
Institute of New Drug Research, Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine, Jinan University College of Pharmacy, Guangzhou 510632, China.
Yuqiang
Y
Wang
Institute of New Drug Research, Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine, Jinan University College of Pharmacy, Guangzhou 510632, China. Electronic address: pennypeiyu@163.com.
Pei
P
Yu
D000098:Acetophenones / Q000008:administration & dosage* / Q000737:chemistry; D055371:Acute Lung Injury / Q000139:chemically induced / Q000188:drug therapy*; D000818:Animals; D002199:Capillary Permeability / Q000187:drug effects; D045424:Complex Mixtures / Q000008:administration & dosage*; D006801:Humans; D007962:Leukocytes / Q000187:drug effects* / Q000473:pathology; D008070:Lipopolysaccharides / Q000276:immunology; D008297:Male; D019255:NADPH Oxidases / Q000378:metabolism; D009589:Nitrogen Oxides / Q000008:administration & dosage*; D010750:Phosphoproteins / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D000098:Acetophenones; D045424:Complex Mixtures; D008070:Lipopolysaccharides; D009589:Nitrogen Oxides; D010750:Phosphoproteins; D014409:Tumor Necrosis Factor-alpha; C079789:neutrophil cytosol factor 67K; C015388:nitrones; C056165:acetovanillone; D013482:Superoxide Dismutase; D019255:NADPH Oxidases; C060505:neutrophil cytosolic factor 1
Acute lung injury; Apocynin; LPS; NADPH oxidase; ROS