Glutathione and glutathione-related enzymes in rats exposed to dimethoate and/or pyrantel.
The study was undertaken to examine the effect of single and combined administration of dimethoate (an OP insecticide) and pyrantel embonate (an anthelmintic agent) on the concentration of reduced glutathione (GSH) and the activity of glutathione peroxidase (GPx) and glutathione reductase (GR) in rats. Dimethoate (Group I) was administered to rats at a dose of 1/10 LD50 for 5 consecutive days and pyrantel embonate (Group II) at a dose of 1/5 LD50 for 3 consecutive days. The animals of group III were given both of the mentioned above compounds in the same manner as group I and II, but pyrantel embonate was applied on day 3, 4, and 5 from the beginning of dimethoate intoxication. Material from 6 rats randomly selected from each group was obtained after 3, 6 and 12 hours and 2, 7 and 14 days following the last applied dose of the compounds under study. It was found that application of pyrantel embonate caused only slight changes in the analysed parameters i.e. GSH, GPx and GR. Dimethoate administration caused disturbances in the antioxidative system manifested as a decrease in GSH concentration in the liver (max.--37.7% after 6 hours) and an increase of GPx and GR activities in erythrocytes (max.--21.7% and 29.6% after 3 hours, respectively), compared to the control group. The profile of changes after combined intoxication was similar, but their intensity was higher compared to the group of animals exposed to dimethoate only. Based on current studies, it was concluded that both dimethoate and pyrantel embonate at the applied doses showed a pro-oxidative activity.
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Spodniewska
D000818:Animals; D000969:Antinematodal Agents / Q000009:adverse effects / Q000097:blood / Q000493:pharmacokinetics; D004117:Dimethoate / Q000009:adverse effects / Q000097:blood / Q000493:pharmacokinetics*; D004347:Drug Interactions; D005978:Glutathione / Q000378:metabolism*; D005979:Glutathione Peroxidase / Q000378:metabolism; D007306:Insecticides / Q000009:adverse effects / Q000097:blood / Q000493:pharmacokinetics; D008099:Liver / Q000187:drug effects; D008297:Male; D011716:Pyrantel Pamoate / Q000009:adverse effects / Q000097:blood / Q000493:pharmacokinetics*; D017208:Rats, Wistar
D000969:Antinematodal Agents; D007306:Insecticides; D011716:Pyrantel Pamoate; D005979:Glutathione Peroxidase; D005978:Glutathione; D004117:Dimethoate