Antioxidant and antifungal activities of Smilax campestris Griseb. (Smilacaceae).
Ethanol extract and fractions obtained from aerial parts of Smilax campestris were examined in order to determine their phenolic composition, antioxidant capacity and antifungal activities. High-performance liquid chromatography coupled with DAD analysis indicated that quercetin and rutin were the main phenolic compounds present in butanol fraction and ethanol extract, respectively. The antioxidant activity assessed by the scavenging ability on 1,1-diphenyl-2-picrylhydrazyl radical was significantly more pronounced for the ethanol extract and butanol fraction than that of the commercial antioxidant 2,6-di-tert-butyl-4-methylphenol. The antifungal activity of extract and fractions was investigated by using microdilution method against five Candida and two Cryptococcus yeast strains. Ethanol extract and fractions exhibited antifungal activities against Candida albicans, Candida glabrata, Candida krusei, Candida parapsilosis, Candida tropicalis and Cryptococcus gattii. This work provides the knowledge of profile and content of flavonoids and their antioxidant and antifungal activities in the extract and fractions of aerial parts of S. campestris.
a Campus Centro-Oeste Dona Lindu, Universidade Federal de São João Del-Rei , Rua Sebastião Gonçalves Coelho 400, Chanadour, Divinópolis , MG 35501-296 , Brazil.
Marcela Isis
MI
Morais
Maria Eduarda Amaral
ME
Pinto
Sthéfane Guimarães
SG
Araújo
Ana Hortência Fonsêca
AH
Castro
Joaquim Mauricio
JM
Duarte-Almeida
Luiz Henrique
LH
Rosa
Carlos Augusto
CA
Rosa
Susana
S
Johann
Luciana Alves Rodrigues dos Santos
LA
Lima
D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000494:pharmacology; D002084:Butylated Hydroxytoluene; D002175:Candida / Q000187:drug effects; D003454:Cryptococcus / Q000187:drug effects; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D015394:Molecular Structure; D010636:Phenols / Q000494:pharmacology; D010851:Picrates / Q000494:pharmacology; D032281:Smilax / Q000737:chemistry*
D000935:Antifungal Agents; D000975:Antioxidants; D001713:Biphenyl Compounds; D005419:Flavonoids; D016166:Free Radical Scavengers; D010636:Phenols; D010851:Picrates; D002084:Butylated Hydroxytoluene; C004931:1,1-diphenyl-2-picrylhydrazyl
DPPH; Smilax campestris; antifungal; antioxidant; flavonoids; yeast