Quantifying the forces guiding microbial cell adhesion using single-cell force spectroscopy.
During the past decades, several methods (e.g., electron microscopy, flow chamber experiments, surface chemical analysis, surface charge and surface hydrophobicity measurements) have been developed to investigate the mechanisms controlling the adhesion of microbial cells to other cells and to various other substrates. However, none of the traditional approaches are capable of looking at adhesion forces at the single-cell level. In recent years, atomic force microscopy (AFM) has been instrumental in measuring the forces driving microbial adhesion on a single-cell basis. The method, known as single-cell force spectroscopy (SCFS), consists of immobilizing a single living cell on an AFM cantilever and measuring the interaction forces between the cellular probe and a solid substrate or another cell. Here we present SCFS protocols that we have developed for quantifying the cell adhesion forces of medically important microbes. Although we focus mainly on the probiotic bacterium Lactobacillus plantarum, we also show that our procedures are applicable to pathogens, such as the bacterium Staphylococcus epidermidis and the yeast Candida albicans. For well-trained microscopists, the entire protocol can be mastered in 1 week.
Université Catholique de Louvain, Institute of Life Sciences, Louvain-la-Neuve, Belgium.
Audrey
A
Beaussart
Université Catholique de Louvain, Institute of Life Sciences, Louvain-la-Neuve, Belgium.
Sofiane
S
El-Kirat-Chatel
Université Catholique de Louvain, Institute of Life Sciences, Louvain-la-Neuve, Belgium.
Ruby May A
RM
Sullan
Université Catholique de Louvain, Institute of Life Sciences, Louvain-la-Neuve, Belgium.
David
D
Alsteens
Université Catholique de Louvain, Institute of Life Sciences, Louvain-la-Neuve, Belgium.
Philippe
P
Herman
Université Catholique de Louvain, Institute of Life Sciences, Louvain-la-Neuve, Belgium.
Sylvie
S
Derclaye
Université Catholique de Louvain, Institute of Life Sciences, Louvain-la-Neuve, Belgium.
Yves F
YF
Dufrêne
D001696:Biomechanical Phenomena; D002176:Candida albicans / Q000502:physiology / Q000648:ultrastructure; D002448:Cell Adhesion / Q000502:physiology*; D048191:Lactiplantibacillus plantarum / Q000502:physiology* / Q000648:ultrastructure; D018625:Microscopy, Atomic Force / Q000379:methods*; D059010:Single-Cell Analysis / Q000379:methods*; D013212:Staphylococcus epidermidis / Q000502:physiology / Q000648:ultrastructure