Spirosoma arcticum sp. nov., isolated from high Arctic glacial till.
A novel orange-pigmented strain, designated R2-35(T), was isolated from a glacier till near Ny-Alesund, Svalbard Archipelago, Norway. The cells were aerobic, Gram-negative, rod-shaped and sometimes filamentous. Growth occurred at 4-28 °C (optimum, 20 °C), at pH 7.0-9.0 (optimum, pH 8.0) and with 0-1% NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain R2-35(T) belonged to the genus Spirosoma with sequence similarity to related species ranging from 91.65 to 95.19%. Strain R2-35(T) contained C16 : 0 (10.7%), C18 : 0 (9.2%), C16 : 1ω5c (16.5%) and summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c) (24.6%) as the major cellular fatty acids, MK-7 as the major respiratory quinone, and phosphatidylethanolamine as the main polar lipid. The DNA G+C content of strain R2-35(T) was 54.9 mol%. On the basis of phylogenetic, physiological and chemotaxonomic data, strain R2-35(T) is considered to represent a novel species of the genus Spirosoma, for which the name Spirosoma arcticum sp. nov., is proposed, The type strain is R2-35(T) ( = CCTCC AB 2012849(T) = LMG 28141(T)).
China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China; Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Wuhan 430072, China.
Xulu
X
Chang
China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China; Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Wuhan 430072, China.
Fan
F
Jiang
China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China; Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Wuhan 430072, China.
Tao
T
Wang
China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China; Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Wuhan 430072, China.
Wenjing
W
Kan
China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China; Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Wuhan 430072, China.
Zhihao
Z
Qu
China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China; Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Wuhan 430072, China.
Lvzhi
L
Ren
China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China; Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Wuhan 430072, China.
Chengxiang
C
Fang
China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China; Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Wuhan 430072, China.
Fang
F
Peng
D001110:Arctic Regions; D015373:Bacterial Typing Techniques; D001482:Base Composition; D041964:Cytophagaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D046448:Ice Cover; D008969:Molecular Sequence Data; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010802:Phylogeny; D010858:Pigmentation; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013538:Svalbard; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C483858:phosphatidylethanolamine; C062629:menaquinone 7