Intravesical administration of green tea extract attenuates the inflammatory response of bacterial cystitis--a rat model.
OBJECTIVE
To explore the effect of intravesical instillation of green tea extract (GTE) on a rat model of bacterial cystitis.


METHODS
In vitro bactericidal properties of GTE were analysed by adding GTE to a suspension of uropathogenic E. coli (UPEC), streaking on MacConkey agar, and incubating overnight. In vivo effects of intravesical instillation of GTE on bacterial cystitis was analysed using a rat model of bacterial cystitis. In all, 42 female Sabra rats weighing 200-260 g were divided into five groups. Parameters measured were bladder weight (percentage of the total rat weight), dipstick urine analysis and histopathological changes in the bladder. Histological changes evaluated were degree of oedema, mixed inflammatory infiltration, urothelial epithelial invasion by neutrophils and reactive atypia.


RESULTS
No in vitro bactericidal activity was detected for GTE. Intravesical instillation of GTE did not cause damage to the rat bladders. Intravesical instillation of GTE attenuated the inflammatory response to UPEC-SR71-induced bacterial cystitis in this rat model.


CONCLUSIONS
Intravesical instillation of GTE attenuated the inflammatory response to UPEC-SR71-induced bacterial cystitis and is a novel approach to the treatment of bacterial cystitis. High concentrations of intravesical GTE did not cause histologically evident damage to the rat bladder. The results of this study are preliminary and further studies will be needed to explore the feasibility of using this approach in humans.
Department of Urology, Hadassah Medical Center, Hebrew University, Jerusalem, Israel.
Shilo
S
Rosenberg
Ray
R
Horowitz
Shunit
S
Coppenhagen-Glazer
Galina
G
Pizov
Anna
A
Elia
Ofer N
ON
Gofrit
Isaac
I
Ginsburg
Dov
D
Pode
D000283:Administration, Intravesical; D000818:Animals; D028241:Camellia sinensis; D003556:Cystitis / Q000188:drug therapy* / Q000382:microbiology; D004195:Disease Models, Animal; D004927:Escherichia coli Infections / Q000188:drug therapy*; D005260:Female; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage*; D018515:Plant Leaves; D051381:Rats
D010936:Plant Extracts
bacterial cystitis; green tea extract; intravesical instillation; uropathogenic E. coli (UPEC)