The role of ROS in hydroquinone-induced inhibition of K562 cell erythroid differentiation.
The role of ROS in hydroquinone-induced inhibition of K562 cell erythroid differentiation was investigated. After K562 cells were treated with hydroquinone for 24 h, and hemin was later added to induce erythroid differentiation for 48 h, hydroquinone inhibited hemin-induced hemoglobin synthesis and mRNA expression of γ-globin in K562 cells in a concentration-dependent manner. The 24-h exposure to hydroquinone also caused a concentration-dependent increase at an intracellular ROS level, while the presence of N- acetyl-L-cysteine prevented hydroquinone- induced ROS production in K562 cells. The presence of N-acetyl-L-cysteine also prevented hydroquinone inhibiting hemin-induced hemoglobin synthesis and mRNA expression of γ-globin in K562 cells. These evidences indicated that ROS production played a role in hydroquinone-induced inhibition of erythroid differentiation.
School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China.
Chun Hong
CH
Yu
School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China.
Suriguga
School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China.
Yang
Y
Li
School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China.
Yi Ran
YR
Li
School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China.
Ke Ya
KY
Tang
School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China.
Liang
L
Jiang
School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China.
Zong Chun
ZC
Yi
D000111:Acetylcysteine / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D006427:Hemin / Q000494:pharmacology; D006801:Humans; D006873:Hydroquinones / Q000494:pharmacology*; D020014:K562 Cells / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism*; D055545:gamma-Globins / Q000235:genetics
D006873:Hydroquinones; D017382:Reactive Oxygen Species; D055545:gamma-Globins; D006427:Hemin; D000111:Acetylcysteine; C031927:hydroquinone