Determination of EBV serostatus prior to kidney transplantation: comparison of VIDAS®, LIAISON® and immunofluorescence assays.
Immunosuppression following solid organ transplantation reduces T cell-mediated immune control of Epstein-Barr Virus (EBV), which may then drive development of post-transplant lymphoproliferative disease. Serology plays a key role in determination of risk of outgrowth of such lesions following transplantation. The study compared the VIDAS(®) (bioMérieux) and LIAISON(®) (DiaSorin) enzyme immunoassays (EIAs) and immunofluorescence assays (IFA; MBL-Bion) in the kidney transplantation setting. Sera from 100 live kidney donors [51 males; age range 20-82 years (mean 51.2 years)] and 100 cadaveric kidney recipients [70 males; age range 17-77 years (mean 51.0 years)] were tested. Overall proportional agreement ranged from 96% to 100% for VIDAS(®) and LIAISON(®). Sensitivity ranged from 91% to 100% and 92% to 100% for VIDAS(®)/IFA and LIAISON(®)/IFA, respectively. The VIDAS(®) and LIAISON(®) approaches gave similar results. Such automated random access EIAs are well suited to busy clinical virology laboratories and rapid determination of donor and recipient EBV serostatus prior to transplantation.
Department of Laboratory Medicine (Virology), Royal Infirmary of Edinburgh, Little France, Edinburgh EH16 4SA, UK. Electronic address: i.johannessen@ed.ac.uk.
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Johannessen
Department of Laboratory Medicine (Virology), Royal Infirmary of Edinburgh, Little France, Edinburgh EH16 4SA, UK.
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Noel
Department of Laboratory Medicine (Virology), Royal Infirmary of Edinburgh, Little France, Edinburgh EH16 4SA, UK.
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Galloway
Department of Laboratory Medicine (Virology), Royal Infirmary of Edinburgh, Little France, Edinburgh EH16 4SA, UK.
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Black
Department of Laboratory Medicine (Virology), Royal Infirmary of Edinburgh, Little France, Edinburgh EH16 4SA, UK.
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Shearman
Epidemiology and Statistics Core, Wellcome Trust Clinical Research Facility, Western General Hospital, Edinburgh EH4 2XU, UK.
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Graham
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000914:Antibodies, Viral / Q000097:blood*; D057205:Automation, Laboratory / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D020031:Epstein-Barr Virus Infections / Q000175:diagnosis*; D005260:Female; D005455:Fluorescent Antibody Technique / Q000379:methods; D004854:Herpesvirus 4, Human / Q000276:immunology*; D006801:Humans; D016030:Kidney Transplantation; D008297:Male; D008875:Middle Aged; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods; D055815:Young Adult
D000914:Antibodies, Viral
Enzyme immunosorbent assay; Epstein–Barr Virus; Immunofluorescent assay; Kidney transplantation; Post-transplant lymphoproliferative disease; Serostatus