A raspberry bushy dwarf virus isolate from Ecuadorean Rubus glaucus contains an additional RNA that is a rearrangement of RNA-2.
Sequencing of the complete genome of a raspberry bushy dwarf virus isolate from Rubus glaucus in Ecuador revealed that its RNA-1 and RNA-2 were 5449 and 2231 nucleotides (nt) long, respectively, and phylogenetically closest to isolates from Sweden and Slovenia. In dsRNA analysis of infected plants, an additional band of 3 kbp was observed. Sequencing of this band revealed that it was 3279 nt long. BLAST searches revealed that this band contained a modified version of RNA-2, which consisted of RNA-2 (2231 nt) plus an additional 1048-nt fragment that was concatenated in a reverse-complement fashion to its 5' terminus.
Centro de Investigaciones Biotecnologicas del Ecuador, Escuela Superior Politecnica del Litoral (CIBE-ESPOL), Km 30.5 Via Perimetral Campus Gustavo Galindo. Apartado 09-01-5863, Guayaquil, Ecuador, diego.quito.avila@gmail.com.
D F
DF
Quito-Avila
M A
MA
Ibarra
R
R
Alvarez
E L
EL
Peralta
R R
RR
Martin
D000818:Animals; D016000:Cluster Analysis; D004484:Ecuador; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D012328:RNA Viruses / Q000235:genetics* / Q000302:isolation & purification*; D012367:RNA, Viral / Q000235:genetics*; D011995:Recombination, Genetic; D027824:Rosaceae / Q000821:virology*; D017422:Sequence Analysis, DNA
D012367:RNA, Viral