Expression of cancer-related carbonic anhydrases IX and XII in normal skin and skin neoplasms.
Purpose of the study was to evaluate the presence of hypoxia-inducible, tumour-associated carbonic anhydrases IX and XII in normal skin and a series of cutaneous tumours. Human tumour samples were taken during surgical operations performed on 245 patients and were immunohistochemically stained. A histological score value was calculated for statistical analyses which were performed using SPSS for Windows, versions 17.0 and 20.0. In normal skin, the highest expression of CA IX was detected in hair follicles, sebaceous glands, and basal parts of epidermis. CA XII was detected in all epithelial components of skin. Both CA IX and CA XII expression levels were significantly different in epidermal, appendigeal, and melanocytic tumour categories. Both CA IX and XII showed the most intense immunostaining in epidermal tumours, whereas virtually all melanocytic tumours were devoid of CA IX and XII immunostaining. In premalignant lesions, CA IX expression significantly increased when the tumours progressed to more severe dysplasia forms. Both CA IX and XII are highly expressed in different epithelial components of skin. They are also highly expressed in epidermal tumours, in which CA IX expression levels also correlate with the dysplasia grade. Interestingly, both isozymes are absent in melanocytic tumours.
Institute of Biomedical Technology and School of Medicine, University of Tampere, Tampere, Finland.
Leo
L
Syrjänen
Tiina
T
Luukkaala
Mari
M
Leppilampi
Matti
M
Kallioinen
Silvia
S
Pastorekova
Jaromir
J
Pastorek
Abdul
A
Waheed
William S
WS
Sly
Seppo
S
Parkkila
Tuomo
T
Karttunen
D049309:Acanthoma / Q000473:pathology; D000951:Antigens, Neoplasm / Q000096:biosynthesis*; D014408:Biomarkers, Tumor; D000071231:Carbonic Anhydrase IX; D002256:Carbonic Anhydrases / Q000096:biosynthesis*; D002280:Basal Cell Carcinoma / Q000473:pathology; D002281:Carcinoma, Basosquamous / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000473:pathology; D006801:Humans; D008545:Melanoma / Q000473:pathology; D009182:Mycosis Fungoides / Q000473:pathology; D012867:Skin / Q000378:metabolism*; D015595:Skin Aging; D012878:Skin Neoplasms / Q000473:pathology*; D013194:Staining and Labeling; D013472:Sunlight / Q000009:adverse effects
D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; C089226:CA9 protein, human; D000071231:Carbonic Anhydrase IX; D002256:Carbonic Anhydrases; C477378:carbonic anhydrase XII
CA IX; CA XI; skin; skin neoplasm