Analysis of oxytocin in milk samples and intake pattern in different age groups of Indian population.
BACKGROUND
Oxytocin (OT) injections have been indiscriminately used to milk cattle in dairy industries. There is no study available regarding surveillance of OT in market milk samples.


METHODS
OT from milk samples was extracted by precipitation with trichloroacetic acid and passed through the solid phase extraction column. OT was eluted and evaporated to dryness under a gentle stream of nitrogen. The residue was either dissolved in milli Q water or buffer for analysis through HPLC or EIA. The intake assessment of OT through milk was assessed through the Food Frequency Recall method employing a Food Frequency Questionnaire. On the basis of milk consumption and the values of OT in milk, the actual intake of OT was calculated.


RESULTS
In the present study, a total of 55 milk samples (39 milkman and 16 branded) were analyzed for occurrence of OT by EIA and UV-HPLC from different locations of Lucknow, Uttar Pradesh (India). OT contamination in milkman samples was found to be 21 pg/mL to 18.9 ng/mL with the mean value of 8.9 ng/mL. The average daily intake of OT in terms of µg/day/person was highest (2.3-2.4 µg/day/person) in 1-3-year age group.


CONCLUSIONS
Since there is no prescribed level of OT in milk and the intake of OT through this commodity is quite high there is need to implement regulatory laws so that non-physiological OT exposure may not occur in children which may have deleterious effects.
Department of Food, Drug and Chemical Toxicology, CSIR-Indian Institute of Toxicology Research (IITR) , Lucknow, Uttar Pradesh , India and.
Manjari
M
Mishra
Shakir
S
Ali
Mukul
M
Das
D000293:Adolescent; D000367:Age Factors; D000818:Animals; D002417:Cattle; D002648:Child; D002675:Child, Preschool; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007194:India; D007223:Infant; D008297:Male; D008892:Milk / Q000737:chemistry*; D010121:Oxytocin / Q000008:administration & dosage / Q000032:analysis*
D010121:Oxytocin
Enzyme immunoassay; SPE column; high-performance liquid chromatography; milk; oxytocin