Photo-patterning of non-fouling polymers and biomolecules on paper.
Functional cellulose substrates with tetrazole moieties are generated to serve as universal platforms for the spatio-temporal immobilization of synthetic ultra-low fouling polymer brushes and protein species via a nitrile imine-mediated tetrazole-ene cycloaddition (NITEC)-based protocol. Poly(carboxybetaine acrylamide) brushes are grafted from initiators photo-patterned by NITEC utilizing single electron transfer living radical polymerization. Streptavidin is photo-immobilized with remarkable efficiency, opening the possibility to generate new materials for biomedical and biosensing applications.
Preparative Macromolecular Chemistry, Institut für Technische Chemie und Polymerchemie, Karlsruhe Institute of Technology (KIT), Engesserstr. 18, 76128, Karlsruhe, Germany; Institut für Biologische Grenzflächen (IBG), Karlsruhe Institute of Technology (KIT), Hermann-von-Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, Germany.
Thomas
T
Tischer
Cesar
C
Rodriguez-Emmenegger
Vanessa
V
Trouillet
Alexander
A
Welle
Vincent
V
Schueler
Jan O
JO
Mueller
Anja S
AS
Goldmann
Eduard
E
Brynda
Christopher
C
Barner-Kowollik
D000327:Adsorption / Q000528:radiation effects; D015374:Biosensing Techniques / Q000379:methods*; D007118:Immunoassay / Q000379:methods*; D008422:Materials Testing; D054802:Molecular Imprinting / Q000379:methods*; D010209:Paper; D010777:Photochemistry / Q000379:methods; D010781:Photography / Q000379:methods*; D040081:Protein Array Analysis / Q000379:methods*; D019809:Streptavidin / Q000737:chemistry*; D013499:Surface Properties / Q000528:radiation effects
D019809:Streptavidin
antifouling polymer brushes; bioactive paper; cellulose; micropatterning; nitrile imine-mediated tetrazole-ene cycloaddition (NITEC)