Cultivation of lipid-producing bacteria with lignocellulosic biomass: effects of inhibitory compounds of lignocellulosic hydrolysates.
Lignocellulosic biomass has been recognized as a promising feedstock for the fermentative production of biofuel. However, the pretreatment of lignocellulose generates a number of by-products, such as furfural, 5-hydroxylmethyl furfural (5-HMF), vanillin, vanillic acids and trans-p-coumaric acid (TPCA), which are known to inhibit microbial growth. This research explores the ability of Rhodococcus opacus PD630 to use lignocellulosic biomass for production of triacylglycerols (TAGs), a common lipid raw material for biodiesel production. This study reports that R. opacus PD630 can grow well in R2A broth in the presence of these model inhibitory compounds while accumulating TAGs. Furthermore, strain PD630 can use TPCA, vanillic acid, and vanillin as carbon sources, but can only use TPCA and vanillic acid for TAG accumulation. Strain PD630 can also grow rapidly on the hydrolysates of corn stover, sorghum, and grass to accumulate TAGs, suggesting that strain PD630 is well-suited for bacterial lipid production from lignocellulosic biomass.
Zachry Department of Civil Engineering, Texas A&M University, College Station, TX 77843, USA.
Baixin
B
Wang
Department of Chemistry, Texas A&M University, College Station, TX 77843-3136, USA.
Yohannes H
YH
Rezenom
Zachry Department of Civil Engineering, Texas A&M University, College Station, TX 77843, USA.
Kun-Ching
KC
Cho
Zachry Department of Civil Engineering, Texas A&M University, College Station, TX 77843, USA.
Janessa L
JL
Tran
Zachry Department of Civil Engineering, Texas A&M University, College Station, TX 77843, USA.
Do Gyun
DG
Lee
Department of Chemistry, Texas A&M University, College Station, TX 77843-3136, USA.
David H
DH
Russell
Center for Phage Technology, Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX 77843-3136, USA.
Jason J
JJ
Gill
Center for Phage Technology, Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX 77843-3136, USA.
Ryland
R
Young
Zachry Department of Civil Engineering, Texas A&M University, College Station, TX 77843, USA. Electronic address: kchu@civil.tamu.edu.
Kung-Hui
KH
Chu
D056804:Biofuels; D018533:Biomass; D006868:Hydrolysis; D008031:Lignin / Q000378:metabolism*; D050356:Lipid Metabolism; D006109:Poaceae; D012240:Rhodococcus / Q000254:growth & development / Q000378:metabolism*; D045868:Sorghum; D014280:Triglycerides / Q000378:metabolism*; D003313:Zea mays
D056804:Biofuels; D014280:Triglycerides; C036909:lignocellulose; D008031:Lignin
Inhibitory compounds; Lignocellulosic biomass; Pretreatment; Rhodococcus opacus PD630; Triacylglycerols (TAGs)