Proteomic Analysis of RBP4/Vitamin A in Children with Cleft Lip and/or Palate.
Cleft of the lip and/or palate (CLP) is one of the most common congenital craniofacial defects. Non-syndromic CLP (NSCLP) is a multifactorial disease influenced by the interaction of genetic and environmental factors. However, there are few studies reporting on the developmental or metabolic status of babies with NSCLP after birth. In our study, we sought to identify and evaluate the differential expression of serum protein profiles in NSCLP children and unaffected babies. Thus, a 'shotgun proteomics' approach was first used to analyze the plasma proteome of 13 children with NSCLP and 10 control children, aged 2 to 3.5 years. In total, more than 300 proteins were identified in the serum sample. With gene ontology (GO) analysis, we detected many differentially expressed proteins that could be related to NSCLP, including those involved in lipoprotein metabolism, insulin-like growth-factor-related processes, and so on, especially the proteins involved in retinol transport. Retinol binding protein 4 (RBP4), one protein of the retinol transport category, was significantly decreased in the NSCLP group. Thus, serum vitamin A levels were further determined by high-performance liquid chromatography (HPLC). A significant difference (p < .01) was also found in vitamin A concentrations, consistent with the trend of RBP4. Our results indicated that reduced levels of RBP4 and vitamin A were related to newborns with NSCLP and should thus receive more attention. These results also suggest that vitamin A supplementation might be necessary at an early stage.
The Department of Orthodontics, Peking University School and Hospital of Stomatology, Beijing, China.
J
J
Zhang
The Department of Orthodontics, Peking University School and Hospital of Stomatology, Beijing, China.
S
S
Zhou
The Center of Laboratory, Peking University School and Hospital of Stomatology, Beijing, China.
Q
Q
Zhang
The School of Life Sciences, Tsinghua University, Beijing, China.
S
S
Feng
The School of Life Sciences, Tsinghua University, Beijing, China.
Y
Y
Chen
The Department of Orthodontics, Peking University School and Hospital of Stomatology, Beijing, China.
H
H
Zheng
The Department of Orthodontics, Peking University School and Hospital of Stomatology, Beijing, China.
X
X
Wang
The Department of Orthodontics, Nanfang Hospital, Southern Medical University, Guangdong, China.
W
W
Zhao
Yantai Stomatology Hospital, Shandong, China.
T
T
Zhang
The Department of Orthodontics, Peking University School and Hospital of Stomatology, Beijing, China.
Y
Y
Zhou
The School of Life Sciences, Tsinghua University, Beijing, China.
H
H
Deng
The Department of Orthodontics, Peking University School and Hospital of Stomatology, Beijing, China jxlin@pku.edu.cn moleculecf@gmail.com.
J
J
Lin
The Center of Laboratory, Peking University School and Hospital of Stomatology, Beijing, China jxlin@pku.edu.cn moleculecf@gmail.com.
F
F
Chen
D001798:Blood Proteins / Q000032:analysis; D015153:Blotting, Western / Q000379:methods; D016022:Case-Control Studies; D002675:Child, Preschool; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002971:Cleft Lip / Q000097:blood*; D002972:Cleft Palate / Q000097:blood*; D005260:Female; D005786:Gene Expression Regulation / Q000235:genetics; D063990:Gene Ontology; D006801:Humans; D008074:Lipoproteins / Q000378:metabolism; D008297:Male; D020543:Proteome / Q000032:analysis*; D054839:Retinol-Binding Proteins, Plasma / Q000032:analysis*; D013002:Somatomedins / Q000502:physiology; D014801:Vitamin A / Q000097:blood* / Q000378:metabolism
D001798:Blood Proteins; D008074:Lipoproteins; D020543:Proteome; C101326:RBP4 protein, human; D054839:Retinol-Binding Proteins, Plasma; D013002:Somatomedins; D014801:Vitamin A
congenital; craniofacial abnormalities; nonsyndromic; proteomics; retinol-binding proteins; vitamins