The preparation, characterization and evaluation of regenerated cellulose/collagen composite hydrogel films.
Porous structured regenerated cellulose films were oxidized by periodate oxidation to obtain 2,3-dialdehyde cellulose (DARC) films, which were then reacted with collagen to obtain DARC/Col composite films. The subsequent FT-IR spectra indicated that collagen was immobilized on the DARC matrix via the Schiff base reaction between NH2 in collagen and CHO in DARC backbone. Scanning electron microscopy revealed that DARC/Col exhibited a refined 3D network structure and its porosity and pore size decreased with increasing of collagen concentration. The composite films demonstrated a good equilibrium-swelling ratio, air permeability and water retention properties. The composite films also showed excellent mechanical properties, which was vital for practical application. Finally, the cytotoxicity of the composite film was evaluated using NIH3T3 mice fibroblast cells, the results revealed that DARC/Col composite films have good biocompatibility for use as scaffold material in tissue engineering.
School of Pharmaceutical Science, Jiangnan University, Wuxi 214122, China.
Yongmei
Y
Cheng
School of Pharmaceutical Science, Jiangnan University, Wuxi 214122, China.
Jinting
J
Lu
College of Food Science & Technology, Huazhong Agricultural University, Wuhan 430070, China.
Shilin
S
Liu
The Third Affiliated Hospital of Nantong University, Wuxi 214041, China.
Peng
P
Zhao
The Third Affiliated Hospital of Nantong University, Wuxi 214041, China.
Guozhong
G
Lu
School of Pharmaceutical Science, Jiangnan University, Wuxi 214122, China. Electronic address: jhchenwhut@126.com.
Jinghua
J
Chen
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D002482:Cellulose / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D003094:Collagen / Q000737:chemistry*; D020100:Hydrogels / Q000737:chemistry*; D055595:Mechanical Phenomena; D051379:Mice; D041681:NIH 3T3 Cells; D016062:Porosity; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*; D014835:Volatilization; D014867:Water / Q000737:chemistry
D001672:Biocompatible Materials; D020100:Hydrogels; D014867:Water; D002482:Cellulose; D003094:Collagen; C058684:2,3-dialdehydocellulose
Cellulose; Collagen; Film; Scaffolds