Catalyzed ester synthesis using Candida rugosa lipase entrapped by poly(N-isopropylacrylamide-co-itaconic acid) hydrogel.
This study reports the synthesis of polymeric matrices based on N-isopropylacrylamide and itaconic acid and its application for immobilization of lipase from Candida rugosa. The lipase was immobilized by entrapment method. Free and immobilized lipase activities, pH and temperature optima, and storage stability were investigated. The optimum temperature for free and entrapped lipase was found to be 40 and 45 °C, while the optimum pH was observed at pH 7 and 8, respectively. Both hydrolytic activity in an aqueous medium and esterolytic activity in an organic medium have been evaluated. Maximum reaction rate (V max) and Michaelis-Menten constants (K m ) were also determined for immobilized lipase. Storage stability of lipase was increased as a result of immobilization process. Furthermore, the operational stability and reusability of the immobilized lipase in esterification reaction have been studied, and it was observed that after 10 cycles, the residual activity for entrapped lipase was as high as 50%, implying that the developed hydrogel and immobilized system could provide a promising solution for the flavor ester synthesis at the industrial scale.
Department of Criminalistic Sciences, The Academy of Criminalistic and Police Studies, Cara Dušana 196, 11080 Belgrade, Serbia.
Nikola
N
Milašinović
Department of Biochemical Engineering and Biotechnology, Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, 11000 Belgrade, Serbia.
Sonja
S
Jakovetić
Department of Biochemical Engineering and Biotechnology, Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, 11000 Belgrade, Serbia.
Zorica
Z
Knežević-Jugović
Department of Organic Chemical Technology, Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, 11000 Belgrade, Serbia.
Nedeljko
N
Milosavljević
Department of Organic Chemical Technology, Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, 11000 Belgrade, Serbia.
Marija
M
Lučić
Department of Organic Chemical Technology, Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, 11000 Belgrade, Serbia.
Jovanka
J
Filipović
Department of Organic Chemical Technology, Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, 11000 Belgrade, Serbia.
Melina
M
Kalagasidis Krušić
D000178:Acrylamides / Q000737:chemistry*; D002175:Candida / Q000737:chemistry* / Q000201:enzymology*; D002384:Catalysis; D004951:Esterification / Q000502:physiology; D004952:Esters; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D008049:Lipase / Q000032:analysis* / Q000378:metabolism; D013386:Succinates / Q000737:chemistry*
D000178:Acrylamides; D004952:Esters; D013386:Succinates; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; C067295:N-isopropylacrylamide; D008049:Lipase; C005229:itaconic acid