Direct patterning of probe proteins on an antifouling PLL-g-dextran coating for reducing the background signal of fluorescent immunoassays.
The limit of detection of advanced immunoassays, biochips and micro/nano biodetection devices is impacted by the non-specific adsorption of target molecules at the sample surface. In this paper, we present a simple and versatile low cost method for generating active surfaces composed of antibodies arrays surrounded by an efficient anti-fouling layer, capable to decrease drastically the fluorescence background signal obtained after interaction with a solution to be analyzed. The technological process involves the direct micro-contact printing of the antibodies probe molecules on a pre-coated PLL-g-dextran thin layer obtained by contact printing using a flat PDMS stamp. Compared to other blocking strategies (ethanolamine blocking treatment, PLL-g-PEG incubation, PLL-g-dextran incubation, printing on a plasma-deposited PEO layer), our surface chemistry method is more efficient for reducing non-specific interactions responsible for a degraded signal/noise ratio.
CNRS, LAAS, 7 avenue du colonel Roche, F-31400, Toulouse, France, aegea@laas.fr.
Amandine M C
AM
Egea
Emmanuelle
E
Trévisiol
Christophe
C
Vieu
D057219:Biofouling / Q000517:prevention & control; D020099:Coated Materials, Biocompatible / Q000737:chemistry* / Q000494:pharmacology; D003911:Dextrans / Q000737:chemistry*; D007118:Immunoassay / Q000379:methods*; D013499:Surface Properties
D020099:Coated Materials, Biocompatible; D003911:Dextrans