Derivation of human decidua-like cells from amnion and menstrual blood.
We induced differentiation of human amnion-derived mesenchymal stem cells (AMCs) and menstrual blood-derived mesenchymal stem cells (MMCs) into endometrial stroma-like cells, which could be useful for cell therapy to support embryo implantation. Interestingly, the expression patterns of surface markers were similar among AMCs, MMCs, and endometrial stromal cells. In addition, whereas treatment with estrogen and progesterone was not very effective for decidualizing AMCs and MMCs, treatment with 8-Br-cAMP prompted remarkable morphological changes in these cells as well as increased expression of decidualization markers (prolactin and insulin-like growth factor binding protein-1) and attenuated expression of surface markers unique to mesenchymal stem cells. These results demonstrated that bone marrow-derived stem cells, which are considered a potential source of endometrial progenitor cells, as well as AMCs and MMCs show in vitro decidualization potential, which is characteristic of endometrial stromal cells.
1] Department of Obstetrics and Gynecology, Keio University School of Medicine, 35 Shinanomachi Shinjuku-ku, Tokyo 160-8582, Japan [2] Department of Reproductive Biology, National Research Institute for Child Health and Development, 2-10-1 Ohkura Setagaya-ku, Tokyo 157-8535, Japan.
Kana
K
Sugawara
Department of Obstetrics and Gynecology, Keio University School of Medicine, 35 Shinanomachi Shinjuku-ku, Tokyo 160-8582, Japan.
Toshio
T
Hamatani
Department of Obstetrics and Gynecology, Keio University School of Medicine, 35 Shinanomachi Shinjuku-ku, Tokyo 160-8582, Japan.
Mitsutoshi
M
Yamada
Department of Obstetrics and Gynecology, Keio University School of Medicine, 35 Shinanomachi Shinjuku-ku, Tokyo 160-8582, Japan.
Seiji
S
Ogawa
Department of Obstetrics and Gynecology, Keio University School of Medicine, 35 Shinanomachi Shinjuku-ku, Tokyo 160-8582, Japan.
Shintaro
S
Kamijo
Department of Obstetrics and Gynecology, Keio University School of Medicine, 35 Shinanomachi Shinjuku-ku, Tokyo 160-8582, Japan.
Naoaki
N
Kuji
Department of Reproductive Biology, National Research Institute for Child Health and Development, 2-10-1 Ohkura Setagaya-ku, Tokyo 157-8535, Japan.
Hidenori
H
Akutsu
Department of Reproductive Biology, National Research Institute for Child Health and Development, 2-10-1 Ohkura Setagaya-ku, Tokyo 157-8535, Japan.
Kenji
K
Miyado
Department of Obstetrics and Gynecology, Keio University School of Medicine, 35 Shinanomachi Shinjuku-ku, Tokyo 160-8582, Japan.
Yasunori
Y
Yoshimura
Department of Reproductive Biology, National Research Institute for Child Health and Development, 2-10-1 Ohkura Setagaya-ku, Tokyo 157-8535, Japan.
Akihiro
A
Umezawa
D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000650:Amnion / Q000166:cytology*; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation; D002460:Cell Line; D003656:Decidua / Q000166:cytology*; D004717:Endometrium / Q000166:cytology; D004967:Estrogens / Q000494:pharmacology; D005260:Female; D006367:HeLa Cells; D006801:Humans; D018970:Insulin-Like Growth Factor Binding Protein 1 / Q000096:biosynthesis; D007633:Keratins / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D008598:Menstruation / Q000097:blood*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D015260:Neprilysin / Q000378:metabolism; D011374:Progesterone / Q000494:pharmacology; D011388:Prolactin / Q000096:biosynthesis; D060245:Tetraspanin 29 / Q000378:metabolism; D014746:Vimentin / Q000378:metabolism
C554869:CD9 protein, human; D004967:Estrogens; D018970:Insulin-Like Growth Factor Binding Protein 1; D008565:Membrane Proteins; D060245:Tetraspanin 29; D014746:Vimentin; D015124:8-Bromo Cyclic Adenosine Monophosphate; D011374:Progesterone; D007633:Keratins; D011388:Prolactin; D015260:Neprilysin