Dendronized nanoconjugates of lysine and folate for treatment of cancer.
Poly-L-lysine (PLL) dendrimers are currently being investigated as antiangiogenic agent for therapy of cancer. In this study, we report folate conjugated poly-l-lysine dendrimers (FPLL) as an efficient carrier for model anticancer drug, doxorubicin hydrochloride (Dox); for pH sensitive drug release, selective targeting to cancer cells, anticancer activity and antiangiogenic activity. This nanoconjugate of Dox showed initial rapid in vitro release followed by gradual slow release, and the drug release was found to be pH sensitive with greater release at acidic pH. In the CAM assay and tubule formation assay with HUVEC, Dox-FPLL formulation showed the significant antiangiogenic activity confirming that activity of PLL was not compromised by the presence of Dox and folic acid. The ex vivo investigations with human breast cancer cell lines MCF-7 showed enhanced cytotoxicity of Dox-FPLL with significantly enhanced intracellular uptake (p<0.001). The in vivo therapeutic potential of nanoconjugate was determined in MCF-7 breast cancer xenograft model in tumor-bearing mice. Dox-FPLL increased the concentration of Dox in tumor by 121.5-fold after 24 h in comparison with free Dox formulation. The folate conjugated dendrimeric Dox showed superior anti-tumor activity in tumor xenograft model with significantly prolonged survival determined by Kaplan Meier survival analysis (p<0.001).
Department of Pharmaceutical Sciences, Dr. H.S. Gour Central University, Sagar, India. Electronic address: keertijain02@gmail.com.
Keerti
K
Jain
Department of Pharmaceutical Sciences, Dr. H.S. Gour Central University, Sagar, India.
Umesh
U
Gupta
Department of Pharmaceutical Sciences, Dr. H.S. Gour Central University, Sagar, India. Electronic address: jnarendr@yahoo.co.in.
Narendra K
NK
Jain
D020533:Angiogenesis Inhibitors / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002626:Chemistry, Pharmaceutical / Q000379:methods; D050091:Dendrimers / Q000737:chemistry*; D004317:Doxorubicin / Q000737:chemistry / Q000494:pharmacology; D004337:Drug Carriers / Q000737:chemistry; D016503:Drug Delivery Systems / Q000379:methods; D005492:Folic Acid / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008239:Lysine / Q000737:chemistry*; D061986:MCF-7 Cells; D051379:Mice; D008807:Mice, Inbred BALB C; D058726:Nanoconjugates / Q000737:chemistry*; D023041:Xenograft Model Antitumor Assays
D020533:Angiogenesis Inhibitors; D000970:Antineoplastic Agents; D050091:Dendrimers; D004337:Drug Carriers; D058726:Nanoconjugates; D004317:Doxorubicin; D005492:Folic Acid; D008239:Lysine
Angiogenesis; Dendrimers; Drug delivery; Folic acid; Lysine; Nanoconjugate