Aggregation/disaggregation of chlorophyll a in model phospholipid-detergent vesicles and micelles.
The photosynthetic pigments of higher plants exist in complex oligomeric states, which are difficult to study in vivo. To investigate aggregation processes of chlorophyll a (Chl a), we used an in vitro reconstitution procedure, with this pigment incorporated into liposomes of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), micelles and pre-micelle media of the detergent n-dodecyltrimethylammonium chloride (DTAC), and mixed, spontaneous, DMPC-DTAC vesicles and micelles. Chl a oligomers were characterized by UV-visible absorption, steady-state and time-resolved fluorescence, and fluorescence lifetime imaging microscopy. Equivalent diameters of the colloidal structures were obtained by fluorescence correlation spectroscopy. In DMPC liposomes and DMPC-DTAC vesicles and micelles, three fluorescence lifetimes indicated the coexistence of Chl a monomers (≈5 ns) and oligomers (≈1-2 to ≈0.1 ns). The increase in DTAC amount, in the mixed system, induces a progressive solubilization of DMPC liposomes (from vesicles to micelles) and simultaneous disruption of Chl a aggregates; in pure DTAC micelles, mostly monomers were found. The present work aims for a better understanding of chlorophyll-chlorophyll (Chl-Chl), Chl-lipid, and Chl-detergent interactions in spontaneous colloidal micro- and nanostructures.
Centro de Química Estrutural, Instituto Superior Técnico, Universidade de Lisboa, 1049-001 Lisboa, Portugal. isabelviseu@tecnico.ulisboa.pt.
Raquel F
RF
Correia
M Isabel
MI
Viseu
Suzana M
SM
Andrade
D000042:Absorption; D002734:Chlorophyll / Q000737:chemistry*; D000077194:Chlorophyll A; D003902:Detergents / Q000737:chemistry*; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry; D005453:Fluorescence; D008081:Liposomes; D008823:Micelles; D008856:Microscopy, Fluorescence; D008956:Models, Chemical; D010743:Phospholipids / Q000737:chemistry*; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013997:Time Factors; D014466:Ultraviolet Rays
D003902:Detergents; D008081:Liposomes; D008823:Micelles; D010743:Phospholipids; D000644:Quaternary Ammonium Compounds; D002734:Chlorophyll; C022806:dodecyltrimethylammonium; D004134:Dimyristoylphosphatidylcholine; D000077194:Chlorophyll A