Helicobacter pylori identification: a diagnostic/confirmatory method for evaluation.
The Helicobacter pylori extra gastric reservoir is probably the oral cavity. In order to evaluate the presence of this bacterium in patients with periodontitis and suspicious microbial cultures, saliva was collected from these and non-periodontitis subjects. PCRs targeting 16S rRNA gene and a 860 bp specific region were performed, and digested with the restriction enzyme DdeI. We observed that the PCR-RFLP approach augments the accuracy from 26.2 % (16/61), found in the PCR-based results, to 42.6 % (26/61), which is an excellent indicator for the establishment of this low-cost procedure as a diagnostic/confirmatory method for H. pylori evaluation.
Instituto de Investigação e Formação Avançada em Técnicas da Saúde (IINFACTS), Instituto Superior de Ciências da Saúde-Norte/CESPU, Gandra PRD, Portugal.
B
B
Mesquita
M J
MJ
Gonçalves
P
P
Pacheco
J
J
Lopes
F
F
Salazar
M
M
Relvas
C
C
Coelho
J J
JJ
Pacheco
C
C
Velazco
D002353:Carrier State / Q000175:diagnosis*; D004269:DNA, Bacterial / Q000235:genetics; D016481:Helicobacter Infections / Q000175:diagnosis; D016480:Helicobacter pylori / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008403:Mass Screening / Q000379:methods; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D009055:Mouth / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012680:Sensitivity and Specificity
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S