[Expressions of protease-activated receptors in human gingival fibroblasts and its functions in periodontitis].
OBJECTIVE
To investigate the expression types of protease-activated receptors(PAR) in human gingival fibroblasts(HGF) and the functions of PAR in periodontitis.


METHODS
Primary HGF were cultured.Reverse transcription PCR(RT-PCR) was used to detect the expression of PAR in HGF. Recombinant gingipain R (rRgp) was applied to HGF. The change of PAR expression on the cell surface was analyzed by real-time quantitative RT-PCR, and enzyme-linked immunosorbent assay (ELISA) was used to detect the change of the interleukin (IL)-6 production from HGF. The results of RT-PCR and ELISA were statistically analyzed using the two independent samples t-test of SPSS10.0 software.


RESULTS
HGF expressed PAR-1 and PAR-3. The expression of PAR-1 and PAR-3 changed after two rRgp treatment with HGF cells. The relative expression of PAR-1 was decreased from 1.04 ± 0.31 to 0.67 ± 0.11 and 0.31 ± 0.11. The relative expression of PAR-3 was decreased from 1.01 ± 0.44 to 0.79 ± 0.13 and 0.44 ± 0.12(P < 0.05). The level of IL-6 was increased after rRgp treatment for 8 h. The control group was (18.77 ± 4.09) µg/L, the rRgp treatment groups were (179.36 ± 15.81) and (320.56 ± 26.19) µg/L respectively.


CONCLUSIONS
HGF expressed PAR-1 and PAR-3 and were involved in periodontal inflammation.
Dental Department, Sir Run Shaw Hospital, School of Medicine,Zhejiang University, Hangzhou 310016, China.
Diya
D
Zhang
Lingjing
L
Hu
Shenglai
S
Li
Lieping
L
Sheng
Zheng
Z
Cao
Department of Oral Medicine, The Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, China, Email: chenlili_1030@163.com.
Lili
L
Chen
D018829:Adhesins, Bacterial; D002462:Cell Membrane; D003546:Cysteine Endopeptidases; D004797:Enzyme-Linked Immunosorbent Assay; D005347:Fibroblasts; D000080867:Gingipain Cysteine Endopeptidases; D005881:Gingiva / Q000378:metabolism*; D006801:Humans; D015850:Interleukin-6; D010518:Periodontitis / Q000378:metabolism*; D044462:Receptors, Proteinase-Activated / Q000096:biosynthesis*
D018829:Adhesins, Bacterial; D000080867:Gingipain Cysteine Endopeptidases; D015850:Interleukin-6; D044462:Receptors, Proteinase-Activated; D003546:Cysteine Endopeptidases