Morphological effects of HA on the cell compatibility of electrospun HA/PLGA composite nanofiber scaffolds.
Tissue engineering is faced with an uphill challenge to design a platform with appropriate topography and suitable surface chemistry, which could encourage desired cellular activities and guide bone tissue regeneration. To develop such scaffolds, composite nanofiber scaffolds of nHA and sHA with PLGA were fabricated using electrospinning technique. nHA was synthesized using precipitation method, whereas sHA was purchased. The nHA and sHA were suspended in PLGA solution separately and electrospun at optimized electrospinning parameters. The composite nanofiber scaffolds were characterized by FE-SEM, EDX analysis, TEM, XRD analysis, FTIR, and X-ray photoelectron. The potential of the HA/PLGA composite nanofiber as bone scaffolds in terms of their bioactivity and biocompatibility was assessed by culturing the osteoblastic cells onto the composite nanofiber scaffolds. The results from in vitro studies revealed that the nHA/PLGA composite nanofiber scaffolds showed higher cellular adhesion, proliferation, and enhanced osteogenesis performance, along with increased Ca(+2) ions release compared to the sHA/PLGA composite nanofiber scaffolds and pristine PLGA nanofiber scaffold. The results show that the structural dependent property of HA might affect its potential as bone scaffold and implantable materials in regenerative medicine and clinical tissue engineering.
Department of Polymer Science and Engineering, School of Applied Chemical Engineering, Kyungpook National University, Daegu 702-701, Republic of Korea.
Adnan
A
Haider
Department of Polymer Science and Engineering, School of Applied Chemical Engineering, Kyungpook National University, Daegu 702-701, Republic of Korea.
Kailash Chandra
KC
Gupta
Department of Polymer Science and Engineering, School of Applied Chemical Engineering, Kyungpook National University, Daegu 702-701, Republic of Korea.
Inn-Kyu
IK
Kang
D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects; D001842:Bone and Bones / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D017886:Durapatite / Q000737:chemistry / Q000627:therapeutic use; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry / Q000627:therapeutic use; D051379:Mice; D057139:Nanofibers / Q000737:chemistry* / Q000627:therapeutic use; D043942:Nanotubes / Q000737:chemistry; D010012:Osteogenesis / Q000187:drug effects*; D011100:Polyglycolic Acid / Q000737:chemistry / Q000627:therapeutic use; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D016062:Porosity; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D017886:Durapatite