Proteomic insight into reduced cell elongation resulting from overexpression of patatin-related phospholipase pPLAIIIδ in Arabidopsis thaliana.
Patatin-containing phospholipase A (pPLA) hydrolyzes membrane glycerolipids, producing free fatty acids and lysoglycerolipids. Ten pPLAs in the Arabidopsis thaliana genome are grouped into 3 subfamilies, and pPLAIIIs differ from pPLAI and IIs in their catalytic motifs and overexpression (OE) of pPLAIIIs reduces cell elongation and cellulose content. To probe the question of how pPLAIII overexpression results in the changes, comparative proteomic analyses were conducted between pPLAIIIδ-OE and WT seedlings. The data indicate a change in the microtubule-associated protein, MAP18. MAP18 is involved in destabilizing cortical microtubules and modulating directional cell growth. The result suggests that pPLAIII and their derived products may regulate cytoskeletal dynamics leading to retardation in anisotropic growth.
Hubei Key Laboratory of Genetic Regulation and Integrative Biology; College of Life Sciences; Huazhong Normal University; Wuhan, PR China; Department of Biology; University of Missouri; St. Louis, MO USA; Donald Danforth Plant Science Center; St. Louis, MO USA.
Yong
Y
Zheng
Department of Biology; University of Missouri; St. Louis, MO USA; Donald Danforth Plant Science Center; St. Louis, MO USA.
Maoyin
M
Li
Department of Biology; University of Missouri; St. Louis, MO USA; Donald Danforth Plant Science Center; St. Louis, MO USA.
Xuemin
X
Wang
D017360:Arabidopsis / Q000201:enzymology* / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000378:metabolism*; D049108:Cell Enlargement; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D010741:Phospholipases A / Q000378:metabolism*; D040901:Proteomics
D029681:Arabidopsis Proteins; C520841:MAP18 protein, Arabidopsis; D008869:Microtubule-Associated Proteins; C585189:pPLAIIIdelta protein, Arabidopsis; D010741:Phospholipases A
cell elongation; cytoskeleton; lipid signaling; microtubule-associated protein; patatin; phopsholipase A