Four-dimensional motility tracking of biological cells by digital holographic microscopy.
Three-dimensional profiling and tracking by digital holography microscopy (DHM) provide label-free and quantitative analysis of the characteristics and dynamic processes of objects, since DHM can record real-time data for microscale objects and produce a single hologram containing all the information about their three-dimensional structures. Here, we have utilized DHM to visualize suspended microspheres and microfibers in three dimensions, and record the four-dimensional trajectories of free-swimming cells in the absence of mechanical focus adjustment. The displacement of microfibers due to interactions with cells in three spatial dimensions has been measured as a function of time at subsecond and micrometer levels in a direct and straightforward manner. It has thus been shown that DHM is a highly efficient and versatile means for quantitative tracking and analysis of cell motility.
University of South Florida, Department of Physics, Digital Holography and Microscopy Laboratory, Tampa, Florida 33620.
Xiao
X
Yu
University of South Florida, Department of Physics, Digital Holography and Microscopy Laboratory, Tampa, Florida 33620.
Jisoo
J
Hong
University of South Florida, Department of Physics, Digital Holography and Microscopy Laboratory, Tampa, Florida 33620.
Changgeng
C
Liu
University of South Florida, Department of Physics, Nanomedicine and Nanobiotechnology Laboratory, Tampa, Florida 33620.
Michael
M
Cross
University of South Florida, Department of Physics, Nanomedicine and Nanobiotechnology Laboratory, Tampa, Florida 33620.
Donald T
DT
Haynie
University of South Florida, Department of Physics, Digital Holography and Microscopy Laboratory, Tampa, Florida 33620.
Myung K
MK
Kim
D002465:Cell Movement / Q000502:physiology*; D058948:Cell Tracking / Q000379:methods*; D044785:Cryptophyta / Q000166:cytology; D006696:Holography / Q000379:methods*; D008954:Models, Biological