Effect of combination of hydrophilic and lipophilic permeation enhancers on the skin permeation of kahalalide F.
OBJECTIVE
The purpose of this study was to investigate the influence of combination of various lipophilic and hydrophilic chemical enhancers on skin delivery of kahalalide F (KF).


METHODS
KF formulations comprising a combination of lipophilic and hydrophilic chemical enhancers with varied per cent were prepared and evaluated for skin permeation studies. In vitro skin permeation of KF formulations was performed using Franz diffusion cell. Stability studies of KF formulations were performed according to the International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use guideline, and the therapeutic efficacy of KF formulation was evaluated using allergic contact dermatitis animal model.


RESULTS
The efficacy of KF formulations to improve skin delivery of KF was sequenced in the order of: formulation #4 > formulation #2 > formulation #1 > formulation #3, where formulation #4 contains labrasol (40% w/v), ethyl oleate (5% w/v) and span 80 (5% w/v) along with transcutol (40% w/v) and ethanol (10% w/v). Further, all the formulations were stable for 1 month when stored at 30°C/65% relative humidity.


CONCLUSIONS
The results of present study suggest that therapeutically effective concentrations of KF can be delivered in the skin using combination of lipophilic and hydrophilic chemical enhancers.
College of Pharmacy and Pharmaceutical Sciences, Florida A&M University, Tallahassee, FL, USA.
Punit P
PP
Shah
Pinaki R
PR
Desai
Ram
R
Patlolla
Larry
L
Klevans
Mandip
M
Singh
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D047630:Depsipeptides / Q000737:chemistry / Q000493:pharmacokinetics*; D017449:Dermatitis, Allergic Contact / Q000188:drug therapy; D004355:Drug Stability; D006801:Humans; D066298:In Vitro Techniques; D051379:Mice; D008810:Mice, Inbred C57BL; D010539:Permeability; D012869:Skin Absorption / Q000187:drug effects*; D012995:Solubility
D047630:Depsipeptides; C097897:kahalalide F
dermatitis; hydrophilic and lipophilic chemical enhancers; kahalalide F; skin inflammation; skin permeation