Analyzed gene expression of oesophageal cells stimulated in a low pH environment by DNA microarray.
OBJECTIVE
The mechanism of gastroesophageal reflux disease (GERD) has gradually been understood at the molecular biological level, and acid is considered as the major cause of GERD. The aim of this study was to explore the molecular mechanism of GERD related with low pH by investigating the differential gene expression of oesophageal cells stimulated under a low pH environment for different time.


METHODS
Comparisons were made between the control normal samples (stimulated for 0 min) and low pH treat samples for various time points, and differentially expressed genes (DEGs) were identified, further bioinformatics analysis were carried out, including functional annotation and PPI network construction.


RESULTS
The result indicated that the number of DEGs was increased along with the time of acid exposure, and the EGR1, JUN and FOS were found in all enriched Gene Ontology terms with association scores between them was high.


CONCLUSIONS
All results suggested that EGR1, JUN, FOS may play important roles in the development of GERD. In a word, our results may reveal the contribution of gastric acid to GERD and the pathogenesis of GERD.
Department of Gastroenterology, Shanghai JiaoTong University Affiliated Sixth People's Hospital, Shanghai, China. yaohuixiang@hotmail.com.
H-X
HX
Yao
W-X
WX
Chen
D002460:Cell Line; D019295:Computational Biology / Q000379:methods; D051766:Early Growth Response Protein 1 / Q000235:genetics; D004947:Esophagus / Q000378:metabolism*; D005744:Gastric Acid / Q000378:metabolism; D005764:Gastroesophageal Reflux / Q000235:genetics*; D015870:Gene Expression / Q000235:genetics*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D016761:Oncogene Proteins v-fos / Q000235:genetics; D016755:Proto-Oncogene Proteins c-jun / Q000235:genetics
C497325:EGR1 protein, human; D051766:Early Growth Response Protein 1; D016761:Oncogene Proteins v-fos; D016755:Proto-Oncogene Proteins c-jun