Isolation of stem cells using spheroids from fresh surgical specimen: an analytic mini-review.
It is a commonly held belief that adult stem cells represent the "seeds" for normal cellular replenishment and also for carcinogenesis. The identification and characterization of stem cells for clinical therapeutic applications, however, is extremely challenging for a number of reasons. Recently, our group and others have attempted to isolate stem cells using spheroids from fresh surgical specimens and utilize them for in vitro and in vivo studies. This mini-review summarizes the major technical steps of these methods along with the primary findings. Besides, it critically analyzes the advantages and limitations of the concept and technical approaches. Finally, this mini-review presents our thoughts on the potential future directions of stem cell isolation and cancer stem cell-related research and clinical applications.
FACS, Executive Medical Director, Bon Secours Cancer Institute, 5855 Bremo Road, Suite 606, Richmond, VA 23226, U.S.A. Tel: +1 8042217364, Itzhak.Avital@gmail.com or Yan-gao Man, Research Lab and International Collaboration Director, Bon Secours Cancer Institute, 5855 Bremo Road, Suite 606, Richmond, VA 23226, U.S.A. yanmann@aol.com.
Itzhak
I
Avital
Alexander
A
Stojadinovic
Hongjian
H
Wang
Ciaran
C
Mannion
Williams C S
WC
Cho
Jinlian
J
Wang
Yan Gao
YG
Man
D000818:Animals; D002469:Cell Separation; D006801:Humans; D009369:Neoplasms / Q000473:pathology* / Q000601:surgery; D014411:Neoplastic Stem Cells / Q000473:pathology*; D018874:Spheroids, Cellular / Q000473:pathology; D014407:Tumor Cells, Cultured
Adult stem cells; carcinogenesis; cellular replenishment; review; spheroid; surgical specimens