Purification and characterization of a novel marine Arthrobacter oxydans KQ11 dextranase.
Dextranases can hydrolyze dextran deposits and have been used in the sugar industry. Microbial strains which produce dextranases for industrial use are chiefly molds, which present safety issues, and dextranase production from them is impractically long. Thus, marine bacteria to produce dextranases may overcome these problems. Crude dextranase was purified by a combination of ammonium sulfate fractionation and ion-exchange chromatography, and then the enzyme was characterized. The enzyme was 66.2 kDa with an optimal temperature of 50°C and a pH of 7. The enzyme had greater than 60% activity at 60°C for 1h. Moreover, 10mM Co(2+) enhanced dextranase activity (196%), whereas Ni(2+) and Fe(3+) negatively affected activity. 0.02% xylitol and 1% alcohol enhanced activity (132.25% and 110.37%, respectively) whereas 0.05% SDS inhibited activity (14.07%). The thickness of S. mutans and mixed-species oral biofilm decreased from 54,340 nm to 36,670 nm and from 64,260 nm to 43,320 nm, respectively.
School of Marine Science and Technology, Huaihai Institute of Technology, Lianyungang, Jiangsu 222005, China; Key Laboratory of Marine Biology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China.
Delong
D
Wang
School of Marine Science and Technology, Huaihai Institute of Technology, Lianyungang, Jiangsu 222005, China; Jiangsu Marine Resources Development Research Insititute, Lianyungang, Jiangsu 222005, China.
Mingsheng
M
Lu
School of Marine Science and Technology, Huaihai Institute of Technology, Lianyungang, Jiangsu 222005, China. Electronic address: shujunwang86@163.com.
Shujun
S
Wang
School of Marine Science and Technology, Huaihai Institute of Technology, Lianyungang, Jiangsu 222005, China.
Yuliang
Y
Jiao
Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, Jiangsu 214122, China.
Weijuan
W
Li
School of Marine Science and Technology, Huaihai Institute of Technology, Lianyungang, Jiangsu 222005, China.
Qiang
Q
Zhu
Key Laboratory of Marine Biology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China. Electronic address: sea@njau.edu.cn.
Zhaopu
Z
Liu
D001173:Arthrobacter / Q000201:enzymology*; D018441:Biofilms; D002852:Chromatography, Ion Exchange; D003910:Dextranase / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D003911:Dextrans / Q000737:chemistry / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008670:Metals / Q000737:chemistry / Q000378:metabolism; D008970:Molecular Weight; D013379:Substrate Specificity; D013696:Temperature
D003911:Dextrans; D008670:Metals; D003910:Dextranase
Dextranase; Marine Arthrobacteroxydans KQ11; Purification and characteristics