Neuroprotective effect of melatonin against kainic acid-induced oxidative injury in hippocampal slice culture of rats.
Endogenous melatonin is a known free radical scavenger that removes reactive oxygen species (ROS), thus, alleviating oxidative stress. The purpose of this study was to demonstrate its effect against kainic acid (KA)-induced oxidative stress in organotypic hippocampal slice cultures (OHSCs). To observe neuroprotective effects of melatonin, different concentrations (0.01, 0.1 and 1 mM) of melatonin were administrated after KA treatment for 18 h in OHSCs of rat pups. Dose-response studies showed that neuronal cell death was significantly reduced after 0.1 and 1 mΜ melatonin treatments based on propidium iodide (PI) uptake and cresyl violet staining. The dichlorofluorescein (DCF) fluorescence which indicates ROS formation decreased more in the melatonin-treated group than in the KA group. The expression of 5-lipoxigenase (5-LO) and caspase-3 were reduced in the melatonin-treated groups compared to the KA group. These results suggest that melatonin may be an effective agent against KA-induced oxidative stress in the OHSC model.
Department of Physiology, Brain Korea 21 Project for Medical Science, Brain Research Institute, Yonsei University College of Medicine, Seoul 120-752, Korea. electrolab@daum.net.
Hyung A
HA
Kim
Division of Health Science, Department of Dental Hygiene, Dongseo University, Busan 617-716, Korea. kyhee@gdsu.dongseo.ac.kr.
Kyung Hee
KH
Lee
Department of Physiology, Brain Korea 21 Project for Medical Science, Brain Research Institute, Yonsei University College of Medicine, Seoul 120-752, Korea. bhlee@yuhs.ac.
Bae Hwan
BH
Lee
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001094:Arachidonate 5-Lipoxygenase / Q000096:biosynthesis; D053148:Caspase 3 / Q000096:biosynthesis; D016923:Cell Death / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D006624:Hippocampus / Q000166:cytology / Q000473:pathology*; D007608:Kainic Acid / Q000633:toxicity*; D008550:Melatonin / Q000494:pharmacology*; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism
D000975:Antioxidants; D018696:Neuroprotective Agents; D017382:Reactive Oxygen Species; D001094:Arachidonate 5-Lipoxygenase; D053148:Caspase 3; D008550:Melatonin; D007608:Kainic Acid