Plasma Membranes Are Subcompartmentalized into a Plethora of Coexisting and Diverse Microdomains in Arabidopsis and Nicotiana benthamiana.
Eukaryotic plasma membranes are highly compartmentalized structures. So far, only a few individual proteins that function in a wide range of cellular processes have been shown to segregate into microdomains. However, the biological roles of most microdomain-associated proteins are unknown. Here, we investigated the heterogeneity of distinct microdomains and the complexity of their coexistence. This diversity was determined in living cells of intact multicellular tissues using 20 different marker proteins from Arabidopsis thaliana, mostly belonging to the Remorin protein family. These proteins associate with microdomains at the cytosolic leaflet of the plasma membrane. We characterized these membrane domains and determined their lateral dynamics by extensive quantitative image analysis. Systematic colocalization experiments with an extended subset of marker proteins tested in 45 different combinations revealed the coexistence of highly distinct membrane domains on individual cell surfaces. These data provide valuable tools to study the lateral segregation of membrane proteins and their biological functions in living plant cells. They also demonstrate that widely used biochemical approaches such as detergent-resistant membranes cannot resolve this biological complexity of membrane compartmentalization in vivo.
Ludwig-Maximilians-University of Munich, Faculty of Biology, Institute of Genetics, 82152 Martinsried, Germany.
Iris K
IK
Jarsch
Ludwig-Maximilians-University of Munich, Faculty of Biology, Institute of Genetics, 82152 Martinsried, Germany.
Sebastian S A
SS
Konrad
Ludwig-Maximilians-University of Munich, Faculty of Biology, Institute of Genetics, 82152 Martinsried, Germany.
Thomas F
TF
Stratil
Ludwig-Maximilians-University of Munich, Faculty of Biology, Institute of Genetics, 82152 Martinsried, Germany.
Susan L
SL
Urbanus
Max-Planck-Institute of Molecular Plant Physiology, 14476 Potsdam-Golm, Germany.
Witold
W
Szymanski
Department of Plant Systems Biology, Center for Life and Food Sciences Weihenstephan, Technische Universität München, 85354 Freising-Weihenstephan, Germany.
Pascal
P
Braun
Ludwig-Maximilians-University of Munich, Faculty of Biology, Institute of Genetics, 82152 Martinsried, Germany.
Karl-Heinz
KH
Braun
Ludwig-Maximilians-University of Munich, Faculty of Biology, Institute of Genetics, 82152 Martinsried, Germany thomas.ott@bio.lmu.de.
Thomas
T
Ott