Inferring the gene network underlying the branching of tomato inflorescence.
The architecture of tomato inflorescence strongly affects flower production and subsequent crop yield. To understand the genetic activities involved, insight into the underlying network of genes that initiate and control the sympodial growth in the tomato is essential. In this paper, we show how the structure of this network can be derived from available data of the expressions of the involved genes. Our approach starts from employing biological expert knowledge to select the most probable gene candidates behind branching behavior. To find how these genes interact, we develop a stepwise procedure for computational inference of the network structure. Our data consists of expression levels from primary shoot meristems, measured at different developmental stages on three different genotypes of tomato. With the network inferred by our algorithm, we can explain the dynamics corresponding to all three genotypes simultaneously, despite their apparent dissimilarities. We also correctly predict the chronological order of expression peaks for the main hubs in the network. Based on the inferred network, using optimal experimental design criteria, we are able to suggest an informative set of experiments for further investigation of the mechanisms underlying branching behavior.
Biometris, Wageningen University and Research Centre, Wageningen, The Netherlands; Netherlands Consortium for Systems Biology, Amsterdam, The Netherlands.
Laura
L
Astola
Biometris, Wageningen University and Research Centre, Wageningen, The Netherlands.
Hans
H
Stigter
Biometris, Wageningen University and Research Centre, Wageningen, The Netherlands; Netherlands Consortium for Systems Biology, Amsterdam, The Netherlands.
Aalt D J
AD
van Dijk
Netherlands Consortium for Systems Biology, Amsterdam, The Netherlands; Keygene N.V., Wageningen, The Netherlands.
Raymond
R
van Daelen
Biometris, Wageningen University and Research Centre, Wageningen, The Netherlands; Netherlands Consortium for Systems Biology, Amsterdam, The Netherlands.
Jaap
J
Molenaar
D035264:Flowers / Q000033:anatomy & histology* / Q000254:growth & development*; D018506:Gene Expression Regulation, Plant; D053263:Gene Regulatory Networks; D017343:Genes, Plant; D005838:Genotype; D056626:Inflorescence / Q000235:genetics*; D018551:Solanum lycopersicum / Q000235:genetics* / Q000254:growth & development; D010641:Phenotype