Molecular characterization of a wheat-Psathyrostachys huashanica Keng 2Ns disomic addition line with resistance to stripe rust.
We characterized a wheat-Psathyrostachys huashanica derived line 3-6-4-1 based on genomic in situ hybridization (GISH), molecular marker analysis, and agronomic trait evaluations. The GISH investigations showed that the 3-6-4-1 contained 42 wheat chromosomes and a pair of P. huashanica chromosomes. The homoeologous relationships of the introduced P. huashanica chromosomes were determined using EST-STS multiple loci markers from seven wheat homoeologous groups in the parents and the addition line. Twelve EST-STS markers located on the homoeologous group 2 chromosomes of wheat amplified polymorphic bands in 3-6-4-1, which were unique to P. huashanica. An introduced Ns chromosome pair that belonged to homoeologous group 2 was identified using chromosome-specific markers. Inoculation with isolates of the stripe rust pathotypes, CYR31, CYR32, and SY11-14, and mixed races (CYR31, CYR32, and SY11-14) in the seeding and adult stage, respectively, showed that 3-6-4-1 was generally resistant to stripe rust, which was probably attributable to its P. huashanica parent. We also compared a complete set of wheat-P. huashanica disomic addition lines (1Ns-7Ns, 2n = 44 = 22II) to assess their agronomic traits and morphological characteristics, which showed that 3-6-4-1 had improved spike traits compared with its parents. The P. huashanica 2Ns chromosome-specific molecular markers in 3-6-4-1 could be useful for marker-assisted selection in breeding programs to combat stripe rust. This line can be used as a donor source to introduce novel excellent genes from P. huashanica into wheat to widen its genetic diversity, thereby providing new germplasms for wheat breeding.
Shaanxi Key Laboratory of Genetic Engineering for Plant Breeding, College of Agronomy, Northwest A&F University, Yangling, 712100, Shaanxi, China, dwl2105@163.com.
Wanli
W
Du
Jing
J
Wang
Liangming
L
Wang
Jun
J
Wu
Jixin
J
Zhao
Shuhui
S
Liu
Qunhui
Q
Yang
Xinhong
X
Chen
D001483:Base Sequence; D001487:Basidiomycota / Q000502:physiology; D032461:Chromosomes, Plant / Q000235:genetics; D004171:Diploidy; D060467:Disease Resistance / Q000235:genetics*; D020224:Expressed Sequence Tags; D017343:Genes, Plant; D008040:Genetic Linkage; D006824:Hybridization, Genetic; D010935:Plant Diseases / Q000382:microbiology*; D036226:Seedlings / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D014908:Triticum / Q000235:genetics* / Q000276:immunology / Q000382:microbiology