Development of a novel anti-canine CD20 monoclonal antibody with diagnostic and therapeutic potential.
In humans, passive immunotherapy with anti-CD20 monoclonal antibodies (mAbs) has created immeasurable improvements in outcomes of patients with B-cell malignancies. However, the lack of comparable reagents has precluded development of this approach in dogs. We developed a novel anti-canine CD20 mAb designated as 6C8. 6C8 recognized the extracellular domain of canine CD20 and showed high-affinity binding to canine CD20 in solution, as well as in its native conformation on canine B-cells. The 6C8 target was expressed invariably in B-cell lineage cells, but not in T-cells or myeloid cells. 6C8 promoted phagocytosis of B-cell lymphoma cells by macrophages, but in its current framework, it did not induce direct cytotoxicity or complement dependent cytotoxicity. In summary, we have established a novel anti-canine CD20 mAb that is useful as a diagnostic tool to phenotype B-cells, and which could be integrated as a tool for passive immunotherapy to treat dogs with B-cell disorders.
Masonic Cancer Center, University of Minnesota , Minneapolis, MN , USA.
Daisuke
D
Ito
Susan
S
Brewer
Jaime F
JF
Modiano
Melissa J
MJ
Beall
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000627:therapeutic use; D000920:Antibody-Dependent Cell Cytotoxicity / Q000276:immunology; D018951:Antigens, CD20 / Q000737:chemistry / Q000276:immunology; D001402:B-Lymphocytes / Q000378:metabolism / Q000473:pathology; D002460:Cell Line; D045744:Cell Line, Tumor; D004285:Dogs; D006801:Humans; D016393:Lymphoma, B-Cell / Q000175:diagnosis / Q000188:drug therapy; D008264:Macrophages / Q000276:immunology; D051379:Mice; D008969:Molecular Sequence Data; D010587:Phagocytosis / Q000276:immunology; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs / Q000276:immunology; D016415:Sequence Alignment
D000911:Antibodies, Monoclonal; D018951:Antigens, CD20
B-cell lymphoma; CD20; dogs; monoclonal antibody; phagocytosis