Macrophage Infiltrate Is Elevated in CRSwNP Sinonasal Tissue Regardless of Atopic Status.
Macrophages are major producers of inflammatory cytokines; however, their role in chronic rhinosinusitis (CRS) has not been clearly defined. The aim of this study was to quantify macrophages in sinus tissue of patients with various subtypes of CRS and determine the impact of atopic status on macrophage infiltrate.



Prospective immunohistochemical study of human sinonasal tissue.



Academic medical center.



Human sinonasal tissue was taken from patients with CRS with nasal polyposis (CRSwNP, n = 8), CRS without nasal polyposis (CRSsNP, n = 8), and controls (n = 8) undergoing surgery for CSF leak repair or endoscopic excision of non-secreting pituitary tumor. Samples were immunohistochemically stained for macrophage/monocyte markers Mac387 and CD68.



CRSwNP patients had significantly increased numbers of Mac387 and CD68 cells compared to control patients (P < .05) or CRSsNP patients (P < .01). CRSsNP had significantly increased number of cells staining for CD68 compared to controls (P < .05). The increased presence of macrophages measured by either marker in CRSwNP was independent of atopic status.



Macrophages are increased in CSRwNP patients regardless of atopic status and may contribute to the immunopathology of CRS.
Department of Otolaryngology, Medical University of South Carolina, Charleston, South Carolina, USA Caroline_Banks@MEEI.HARVARD.EDU.
Caroline A
CA
Banks
Department of Otolaryngology, Medical University of South Carolina, Charleston, South Carolina, USA Department of Surgery, Ralph H. Johnson VA Medical Center, Charleston, South Carolina, USA.
Rodney J
RJ
Schlosser
Department of Otolaryngology, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.
Eric W
EW
Wang
Medical University of South Carolina, Charleston, South Carolina, USA.
Sarah E
SE
Casey
Medical University of South Carolina, Charleston, South Carolina, USA.
Ryan M
RM
Mulligan
Department of Otolaryngology, Medical University of South Carolina, Charleston, South Carolina, USA Ralph H. Johnson VA Medical Center, Research Service, Charleston, South Carolina, USA Department of Pediatrics, Medical University of South Carolina, Charleston, South Carolina, USA.
Jennifer K
JK
Mulligan
D000328:Adult; D015703:Antigens, CD / Q000276:immunology; D015214:Antigens, Differentiation, Myelomonocytic / Q000276:immunology; D015415:Biomarkers / Q000032:analysis; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008264:Macrophages / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D009298:Nasal Polyps / Q000276:immunology*; D011446:Prospective Studies; D012220:Rhinitis / Q000276:immunology*; D012852:Sinusitis / Q000276:immunology*; D000097382:CD68 Molecule
D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D015415:Biomarkers; C067980:CD68 antigen, human; D000097382:CD68 Molecule
atopy; chronic rhinosinusitis; macrophage; nasal polyps