Biomechanical properties: effects of low-level laser therapy and Biosilicate® on tibial bone defects in osteopenic rats.
OBJECTIVE
The aim of this study was to investigate the effects of laser therapy and Biosilicate® on the biomechanical properties of bone callus in osteopenic rats.


METHODS
Fifty female Wistar rats were equally divided into 5 groups (n=10/group): osteopenic rats with intact tibiae (SC); osteopenic rats with unfilled and untreated tibial bone defects (OC); osteopenic rats whose bone defects were treated with Biosilicate® (B); osteopenic rats whose bone defects were treated with 830-nm laser, at 120 J/cm2 (L120) and osteopenic rats whose bone defects were treated with Biosilicate® and 830-nm laser, at 120 J/cm2 (BL120). Ovariectomy (OVX) was used to induce osteopenia. A non-critical bone defect was created on the tibia of the osteopenic animals 8 weeks after OVX. In Biosilicate® groups, bone defects were completely filled with the biomaterial. For the laser therapy, an 830-nm laser, 120 J/cm2 was used. On day 14 postsurgery, rats were euthanized, and tibiae were removed for biomechanical analysis.


RESULTS
Maximal load and energy absorption were higher in groups B and BL120, according to the indentation test. Animals submitted to low-level laser therapy (LLLT) did not show any significant biomechanical improvement, but the association between Biosilicate® and LLLT was shown to be efficient to enhance callus biomechanical properties. Conversely, no differences were found between study groups in the bending test.


CONCLUSIONS
Biosilicate® alone or in association with low level laser therapy improves biomechanical properties of tibial bone callus in osteopenic rats.
1 Department of Physiotherapy, Federal University of São Carlos (UFSCar), São Carlos, SP - Brazil.
Renan
R
Fangel
Paulo S
PS
Bossini
Ana Cláudia
AC
Renno
Renata N
RN
Granito
Charles C
CC
Wang
Keico O
KO
Nonaka
Patricia
P
Driusso
Nivaldo A
NA
Parizotto
Jorge
J
Oishi
D000818:Animals; D003131:Combined Modality Therapy / Q000379:methods; D005260:Female; D017102:Fracture Healing; D005898:Glass; D006244:Hardness; D028022:Low-Level Light Therapy / Q000379:methods*; D058866:Osteoporotic Fractures / Q000175:diagnosis / Q000503:physiopathology* / Q000628:therapy*; D051381:Rats; D017208:Rats, Wistar; D013978:Tibial Fractures / Q000175:diagnosis / Q000503:physiopathology* / Q000628:therapy*; D016896:Treatment Outcome; D016474:Weight-Bearing
C120461:sodium oxide-calcium oxide-silicon dioxide-diphosphorus pentoxide