Protein structure determination by MicroED.
In this review we discuss the current advances relating to structure determination from protein microcrystals with special emphasis on the newly developed method called MicroED. This method uses a transmission electron cryo-microscope to collect electron diffraction data from extremely small 3-dimensional (3D) crystals. MicroED has been used to solve the 3D structure of the model protein lysozyme to 2.9Å resolution. As the method further matures, MicroED promises to offer a unique and widely applicable approach to protein crystallography using nanocrystals.
Janelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USA.
Brent L
BL
Nannenga
Janelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USA. Electronic address: gonent@janelia.hhmi.org.
Tamir
T
Gonen
D053000:Analytic Sample Preparation Methods; D020285:Cryoelectron Microscopy / Q000379:methods*; D018360:Crystallography, X-Ray; D011506:Proteins / Q000737:chemistry*
D011506:Proteins