Effects of Nigella sativa, Lepidium sativum and Trigonella foenum-graecum on sildenafil disposition in beagle dogs.
The present study was conducted to investigate the effects of some commonly used herbs namely Nigella sativa, Lepidium sativum and Trigonella foenum-graecum on the pharmacokinetics of sildenafil in beagle dogs. The study design involved four treatments in a non-balanced crossover design. Sildenafil was given one tablet 100 mg orally to each dog and blood samples were obtained. After a suitable washout period, animals were commenced on a specific herb treatment for 1 week. Blood samples were withdrawn at different time intervals and sildenafil was analyzed by HPLC method. Oral administration of Nigella sativa resulted in reduction of AUC0-∞, C max and t 1/2 as compared to the control. Treatment of Lepidium sativum resulted in a significant reduction in the C max and AUC. There were no significant differences between the rests of the pharmacokinetic parameters relative to those of the control. For Trigonella foenum-graecum, the effects were similar to those obtained in case of Lepidium sativum. It was concluded that concurrent use of investigated herbs alters the pharmacokinetics of sildenafil. Co-administration of investigated herbs should be cautious since their concomitant use might result in decrease in sildenafil bioavailability.
Department of Pharmaceutics, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh, 11451, Saudi Arabia.
Abdullah M
AM
Al-Mohizea
Abdul
A
Ahad
Gamal M
GM
El-Maghraby
Fahad I
FI
Al-Jenoobi
Khalid M
KM
AlKharfy
Saleh A
SA
Al-Suwayeh
D000818:Animals; D019540:Area Under Curve; D004285:Dogs; D041743:Herb-Drug Interactions; D031220:Lepidium sativum; D008297:Male; D031881:Nigella sativa; D010879:Piperazines / Q000493:pharmacokinetics*; D011687:Purines / Q000493:pharmacokinetics; D000068677:Sildenafil Citrate; D013449:Sulfonamides / Q000493:pharmacokinetics*; D029910:Trigonella
D010879:Piperazines; D011687:Purines; D013449:Sulfonamides; D000068677:Sildenafil Citrate