[Validation of a liquid chromatography method for rifampicin determination in human plasma].
OBJECTIVE
To validate the high-performance liquid chromatography method (HPLC) for rifampicin (RFP) determination in human plasma.


METHODS
A HPLC method for RFP determination in plasma was developed. The separation was performed by reversed-phase chromatography with C18 column and a mobile phase composed of a mixture of acetonitrile and monobasic potassium phosphate buffer solution 0.05 M (38:62 v/v) at 335 nm in which standard rifampicin quinone (RFP-QN) was used.


RESULTS
The retention times of RFP and RFP-QN were 7.81 and 12.26 minutes, respectively. The trial was linear from 0.5 to 250 ug/mL. The evaluated parameters of precision, accuracy, selectivity, linearity, and recovery complied with the established international standards for validation of bioanalytical methods.


CONCLUSIONS
The developed HPLC method is simple, specific, sensitive, selective and linear for a wide range of RFP concentrations in plasma.
Centro Nacional de Control de Calidad, Instituto Nacional de Salud, Lima, Perú
Luis
L
Moreno-Exebio
Centro Nacional de Control de Calidad, Instituto Nacional de Salud, Lima, Perú
Miguel
M
Grande-Ortiz
D000904:Antibiotics, Antitubercular / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D016903:Drug Monitoring / Q000379:methods*; D006801:Humans; D012293:Rifampin / Q000097:blood*
D000904:Antibiotics, Antitubercular; D012293:Rifampin