Quantitation of oxidative stress gene expression in MCF-7 human cell lines treated with water-dispersible CuO nanoparticles.
The objective of this study was to assess the cytotoxicity of water-dispersible CuO nanoparticles by quantifying the reactive oxygen species (ROS)-related genes (glutathione S-transferase (GST) and catalase) using real-time polymerase chain reaction (RT-PCR). Monodisperse CuO nanoparticles of 14 nm in size were used. Cytotoxicity of CuO nanoparticles was evaluated under in vitro condition at different concentrations (10, 50, and 100 μg/ml) and incubation times (12, 24, and 48 h) with human cancer cell lines (breast cancer epithelial cells). The genetic level cytotoxic screening produced consistent results showing that GST and catalase ROS gene expression was maximized in 24 h incubation at 100 μg/ml concentration of CuO nanoparticles. However, the cytotoxicity of water-dispersible CuO nanoparticle was not significant compared with control experiments, demonstrating its high potential in the application of nanomedicines for a diagnostic and therapeutic tool.
Department of Biological Engineering, Konkuk University, 120 Neungdong-ro, Gwangjin-Gu, Seoul, 143701, Republic of Korea, kumaran.ran@gmail.com.
Rangarajulu Senthil
RS
Kumaran
Yong-Keun
YK
Choi
Hyung Joo
HJ
Kim
Kwang Jin
KJ
Kim
D002374:Catalase / Q000096:biosynthesis*; D045744:Cell Line, Tumor; D003300:Copper / Q000737:chemistry / Q000494:pharmacology; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D005982:Glutathione Transferase / Q000096:biosynthesis*; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D018384:Oxidative Stress / Q000187:drug effects*; D010316:Particle Size
D003300:Copper; D002374:Catalase; D005982:Glutathione Transferase; C030973:cupric oxide