Vicenin 2 isolated from Artemisia capillaris exhibited potent anti-glycation properties.
Vicenin 2, isolated from a traditionally used medicinal plant Artemisia capillaris, is a 6,8-di-C-glucoside of apigenin which has been previously reported to possess a wide variety of pharmacological activities including antioxidant, anti-inflammatory, anti-cancer, and hepatoprotective. However, there have not been any reports concerning its anti-diabetic potential until now. Therefore, in the present study, we evaluated the anti-diabetic potential of vicenin 2 via α-glucosidase, protein tyrosine phosphatase 1B (PTP1B), rat lens aldose reductase (RLAR), and advanced glycation end products (AGE) formation inhibitory assays. Vicenin 2 strongly inhibited α-glucosidase, PTP1B, and RLAR in the corresponding assays. In addition, vicenin 2 inhibited the formation of both fluorescent AGE and nonfluorescent AGE, e.g., CML, as well as the level of fructosamine in glucose-fructose-induced bovine serum albumin (BSA) glycation. In the test system, vicenin 2 suppressed glycation-induced protein oxidation by attenuating the formation of protein carbonyl groups as well as by inhibiting the modification of protein thiol groups. Moreover, vicenin 2 was found to be a potent inhibitor of glycation-induced formation of amyloid cross-β structures in BSA. Taken together, vicenin 2 might be a useful lead for the development of multiple target-oriented therapeutic modalities for the treatment of diabetes and diabetes-associated complications.
Department of Food Science and Nutrition, Pukyong National University, Busan 608-737, Republic of Korea.
Md Nurul
MN
Islam
Department of Food Science and Nutrition, Pukyong National University, Busan 608-737, Republic of Korea.
Ishrat Jahan
IJ
Ishita
Department of Food Science and Human Nutrition, Chonbuk National University, Jeonju 561-756, Republic of Korea. Electronic address: jungha@jbnu.ac.kr.
Hyun Ah
HA
Jung
Department of Food Science and Nutrition, Pukyong National University, Busan 608-737, Republic of Korea. Electronic address: choijs@pknu.ac.kr.
Jae Sue
JS
Choi
D000449:Aldehyde Reductase / Q000037:antagonists & inhibitors / Q000378:metabolism; D000682:Amyloid / Q000737:chemistry / Q000187:drug effects; D000818:Animals; D047310:Apigenin / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D019306:Artemisia / Q000737:chemistry*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D019270:Fructosamine / Q000378:metabolism; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D017127:Glycation End Products, Advanced / Q000187:drug effects / Q000378:metabolism*; D065089:Glycoside Hydrolase Inhibitors / Q000494:pharmacology; D007908:Lens, Crystalline / Q000201:enzymology; D008239:Lysine / Q000031:analogs & derivatives / Q000378:metabolism; D010084:Oxidation-Reduction; D010946:Plants, Medicinal / Q000737:chemistry; D050050:Protein Carbonylation / Q000187:drug effects; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1 / Q000037:antagonists & inhibitors / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism; D000520:alpha-Glucosidases / Q000378:metabolism
D000682:Amyloid; D004791:Enzyme Inhibitors; D005960:Glucosides; D017127:Glycation End Products, Advanced; D065089:Glycoside Hydrolase Inhibitors; C530449:apigenin-6,8-di-C-glycopyranoside; D012710:Serum Albumin, Bovine; D019270:Fructosamine; C048496:N(6)-carboxymethyllysine; D047310:Apigenin; D000449:Aldehyde Reductase; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1; D000520:alpha-Glucosidases; D008239:Lysine
Advanced glycation end products; Aldose reductase; Anti-diabetic; Protein tyrosine phosphatase 1B; Vicenin 2; α-Glucosidase