Protective effect of flavonoid extract from Chinese bayberry (Myrica rubra Sieb. et Zucc.) fruit on alcoholic liver oxidative injury in mice.
To evaluate the beneficial effects of Chinese bayberry (Myrica rubra Sieb. et Zucc.) flavonoid extract (CBFE) on chronic alcohol-induced liver oxidative injury in mice, experimental mice were pretreated with different doses of CBFE (50-200 mg/kg) for 4 weeks by gavage feeding. Biochemical markers and enzymatic antioxidants from serum, liver tissue, mitochondria and microsomes were examined. Our results showed that the activities of TC, TG, L-DLC in serum, the activity of CYP2E1 in microsomes, and the levels of MDA in liver tissue and mitochondria, decreased significantly (P < 0.05) in the CBFE-treated group compared with the alcohol group. On the contrary, the activities of ALT, AST, and H-DLC in serum, enzymatic antioxidants GSH-Px, SOD and GST in liver tissue and mitochondria, and HO-1 in microsomes rose markedly (P < 0.05). Histopathological examination revealed that CBFE (200 mg/kg) pretreatment noticeably prevented alcohol-induced hepatocyte apoptosis and fatty degeneration. It was suggested that the hepatoprotective effects exhibited by CBFE on alcohol-induced liver oxidative injury may be due to its potent antioxidant properties.
College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo, 315100, People's Republic of China.
Hesheng
H
Liu
Xiangyang
X
Qi
Shaoqian
S
Cao
Peipei
P
Li
D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000627:therapeutic use*; D056486:Chemical and Drug Induced Liver Injury / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D000431:Ethanol; D005260:Female; D005419:Flavonoids / Q000627:therapeutic use*; D005638:Fruit / Q000737:chemistry; D008099:Liver / Q000201:enzymology / Q000473:pathology; D051379:Mice; D008862:Microsomes, Liver / Q000201:enzymology; D008928:Mitochondria / Q000201:enzymology; D031641:Myrica / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000627:therapeutic use; D020011:Protective Agents / Q000627:therapeutic use
D000975:Antioxidants; D005419:Flavonoids; D010936:Plant Extracts; D020011:Protective Agents; D000431:Ethanol