[Overexpressed microRNA-93 inhibits the proliferation and promotes apoptosis of A172 glioma cells].
OBJECTIVE
To investigate the impact of microRNA-93 on the biological behaviors of A172 glioma cells by observing the changes of cell proliferation, cell cycle and apoptosis.


METHODS
Real-time quantitative PCR (qRT-PCR) was applied to detect the expression of microRNA-93 in 2 samples of human normal brain tissues, 10 samples of glioma tissues and 5 glioma cell lines. Artificially synthesized microRNA-93 mimic was transiently transfected into A172 glioma cells, and then the expression of microRNA-93 was tested by qRT-PCR. MTT assay was used to detect the cell proliferation of A172 glioma cells; apoptosis and cell cycle of A172 glioma cells were measured by flow cytometry.


RESULTS
MicroRNA-93 was over-expressed in glioma tissues and glioma cell lines as compared with normal samples. The transient transfection of microRNA-93 mimic into A172 glioma cells significantly increased the expression of microRNA-93 in A172 glioma cells, promoted cell proliferation, raised the cell proportion in S phase, reduced the cell proportion in G1 phase, and inhibited cell apoptosis.


CONCLUSIONS
MicroRNA-93 was aberrantly over-expressed in glioma tissues and cell lines. Transient transfection of microRNA-93 mimic led to increased proliferation, G1-to-S cell cycle progression and reduced apoptosis in A172 glioma cells, indicating that micro-RNA-93 might be a new target for the diagnosis and treatment of glioma.
Department of Neurosurgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Fan
F
Yang
Department of Immunology, School of Basic Medicine, Fourth Military Medical University, Xi'an 710032, China.
Wei
W
Wang
Department of Immunology and Pathogenic Biology, School of Medicine, Xi'an Jiaotong University, Xi'an 710061, China.
Lu
L
Yuan
Department of Neurosurgery, Navy General Hospital, Beijing 100037, China.
Chunhui
C
Zhou
Department of Immunology, School of Basic Medicine, Fourth Military Medical University, Xi'an 710032, China.
Wenjin
W
Xi
Department of Urology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Ming
M
Wei
Department of Immunology, School of Basic Medicine, Fourth Military Medical University, Xi'an 710032, China.
Guoxu
G
Zheng
Department of Immunology, School of Basic Medicine, Fourth Military Medical University, Xi'an 710032, China.
Angang
A
Yang
Department of Neurosurgery, Navy General Hospital, Beijing 100037, China.
Jianning
J
Zhang
Department of Immunology, School of Basic Medicine, Fourth Military Medical University, Xi'an 710032, China.
Tao
T
Wang
D017209:Apoptosis / Q000235:genetics*; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000235:genetics / Q000473:pathology*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*
C543350:MIRN93 microRNA, human; D035683:MicroRNAs