Synthesis and identification of proposed biosynthetic intermediates of saxitoxin in the cyanobacterium Anabaena circinalis (TA04) and the dinoflagellate Alexandrium tamarense (Axat-2).
Here, we describe the synthesis of the genetically predicted biosynthetic intermediates of the neurotoxin saxitoxin (STX) (1), 2, 6 and 7, and identification of 2 and 6 in toxin-producing microorganisms. This is the first chemical evidence supporting the genetically predicted biosynthetic route toward 1.
Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiya, Aoba-ku, Sendai 981-8555, Japan. myama@biochem.tohoku.ac.jp.
Shigeki
S
Tsuchiya
Yuko
Y
Cho
Keiichi
K
Konoki
Kazuo
K
Nagasawa
Yasukatsu
Y
Oshima
Mari
M
Yotsu-Yamashita
D017033:Anabaena / Q000378:metabolism*; D053898:Biosynthetic Pathways; D002853:Chromatography, Liquid; D004141:Dinoflagellida / Q000378:metabolism*; D007202:Indicators and Reagents; D013058:Mass Spectrometry; D012530:Saxitoxin / Q000096:biosynthesis* / Q000737:chemistry
D007202:Indicators and Reagents; D012530:Saxitoxin