A fast and mild decellularization protocol for obtaining extracellular matrix.
Degradation of extracellular matrix (ECM) function with age is a major cause of loss of tissue function with age that we would wish to reverse. Tissue engineering to provide replacement tissue requires an ECM-mimicking scaffold for cell organization. The standard protocols for achieving this take 10 days and include steps that may change the protein structure of the ECM. Here we describe a much shorter protocol for decellularizing chicken muscle, skin, and tendon samples that achieves the same efficiency as the original protocol without protein cross-link interference. Our protocol can be completed in 72 hr.
Department of Chemical Engineering and Biotechnology, University of Cambridge , Cambridge, United Kingdom .
Ariana
A
Mirzarafie
Rhian K
RK
Grainger
Ben
B
Thomas
William
W
Bains
Fatma I
FI
Ustok
Chris R
CR
Lowe
D000818:Animals; D002645:Chickens; D004247:DNA / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism*; D013710:Tendons / Q000166:cytology; D023822:Tissue Engineering / Q000379:methods*
D004247:DNA