Development of drug-loaded chitosan-vanillin nanoparticles and its cytotoxicity against HT-29 cells.
Chitosan as a natural polysaccharide derived from chitin of arthropods like shrimp and crab, attracts much interest due to its inherent properties, especially for application in biomedical materials. Presently, biodegradable and biocompatible chitosan nanoparticles are attractive for drug delivery. However, some physicochemical characteristics of chitosan nanoparticles still need to be further improved in practice. In this work, chitosan nanoparticles were produced by crosslinking chitosan with 3-methoxy-4-hydroxybenzaldehyde (vanillin) through a Schiff reaction. Chitosan nanoparticles were 200-250 nm in diameter with smooth surface and were negatively charged with a zeta potential of - 17.4 mV in neutral solution. Efficient drug loading and drug encapsulation were achieved using 5-fluorouracil as a model of hydrophilic drug. Drug release from the nanoparticles was constant and controllable. The in vitro cytotoxicity against HT-29 cells and cellular uptake of the chitosan nanoparticles were evaluated by methyl thiazolyl tetrazolium method, confocal laser scanning microscope and flow cytometer, respectively. The results indicate that the chitosan nanoparticles crosslinked with vanillin are a promising vehicle for the delivery of anticancer drugs.
a Agricultural Product Processing Research Institute, Chinese Academy of Tropical Agricultural Sciences , Zhanjiang , P.R. China .
Pu-Wang
PW
Li
a Agricultural Product Processing Research Institute, Chinese Academy of Tropical Agricultural Sciences , Zhanjiang , P.R. China .
Guang
G
Wang
a Agricultural Product Processing Research Institute, Chinese Academy of Tropical Agricultural Sciences , Zhanjiang , P.R. China .
Zi-Ming
ZM
Yang
b Institute for Frontier Materials , Deakin University , Geelong Waurn Ponds Campus , Australia , and.
Wei
W
Duan
a Agricultural Product Processing Research Institute, Chinese Academy of Tropical Agricultural Sciences , Zhanjiang , P.R. China .
Zheng
Z
Peng
b Institute for Frontier Materials , Deakin University , Geelong Waurn Ponds Campus , Australia , and.
Ling-Xue
LX
Kong
a Agricultural Product Processing Research Institute, Chinese Academy of Tropical Agricultural Sciences , Zhanjiang , P.R. China .|c National Center for Important Tropical Crops Engineering and Technology Research , Haikou , P.R. China.
Qing-Huang
QH
Wang
D000963:Antimetabolites / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics; D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000737:chemistry*; D001547:Benzaldehydes / Q000737:chemistry*; D048271:Chitosan / Q000737:chemistry*; D003432:Cross-Linking Reagents; D004339:Drug Compounding; D016503:Drug Delivery Systems; D005079:Excipients; D005472:Fluorouracil / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics; D019073:HT29 Cells; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size
D000963:Antimetabolites; D000970:Antineoplastic Agents; D001547:Benzaldehydes; D003432:Cross-Linking Reagents; D005079:Excipients; D048271:Chitosan; C100058:vanillin; D005472:Fluorouracil
Chitosan; cytotoxicity; drug delivery system; nanoparticles; vanillin