Photocontrol of AMPA receptors with a photochromic ligand.
Photochromic ligands (PCLs), recently introduced by our group as a tool for researchers in neuroscience, offer the ability to control native receptors with light in a reversible fashion without the need for any genetic manipulation. Here we describe the application of the PCL Azo-Tetrazole-AMPA-3 (ATA-3) to reversibly gate native AMPA-receptors with blue light and thereby control the activity of cortical neurons in brain slices.
Department of Chemistry, Ludwig-Maximilians-Universität München, Butenandtstr. 5-13, 81377, Munich, Germany, martin.sumser@cup.lmu.de.
Martin
M
Sumser
Philipp
P
Stawski
D000818:Animals; D001921:Brain / Q000166:cytology / Q000187:drug effects; D057809:HEK293 Cells; D006801:Humans; D066298:In Vitro Techniques; D015640:Ion Channel Gating / Q000528:radiation effects; D008024:Ligands; D008564:Membrane Potentials; D008810:Mice, Inbred C57BL; D010080:Oxazoles / Q000737:chemistry / Q000494:pharmacology; D018408:Patch-Clamp Techniques; D055668:Photochemical Processes; D018091:Receptors, AMPA / Q000819:agonists* / Q000502:physiology; D013777:Tetrazoles / Q000737:chemistry / Q000494:pharmacology
C575234:ATA-3 (tetrazole); D008024:Ligands; D010080:Oxazoles; D018091:Receptors, AMPA; D013777:Tetrazoles