The immunomodulation of inducible hydrogen sulfide in Pacific oyster Crassostrea gigas.
Hydrogen sulfide (H2S) is an important gasotransmitter, which plays indispensable roles in cardiovascular, nervous and immune systems of vertebrates. However, the information about the immunomodulation of H2S in invertebrates is still very limited. In the present study, the temporal expression profile of cystathionine γ lyase in oyster Crassostrea gigas (CgCSE) was investigated after the oysters were stimulated by lipopolysaccharide. The expression levels of CgCSE mRNA transcripts in hemocytes increased significantly at 12h (1.31-fold of the PBS group, P<0.05) after LPS stimulation. The immunomodulation of inducible H2S in oyster was examined by monitoring the alterations of both cellular and humoral immune parameters in response to the stimulations of LPS, LPS+Na2S and LPS+propargylglycine (PAG). The total hemocyte counts (THC) and hemolymph PO activity increased significantly after LPS stimulation, and the increase could be further enhanced by adding PAG, while inhibited by appending Na2S. The phagocytosis activity of hemocytes was also increased firstly after LPS treatment, and the increase was enhanced by adding Na2S but inhibited after appending PAG. The anti-bacterial activity in hemolymph increased at 3h post LPS treatment, and then decreased after adding PAG. The total SOD activity of hemolymph was also elevated at 6h post LPS treatment, and the elevated activity was depressed by adding Na2S. These results collectively indicated that H2S might play crucial roles in the immune response of oyster via modulating the turnover and phagocytosis of hemocytes, and regulating the anti-bacterial activity and proPO activation in the hemolymph.
Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.
Zhibin
Z
Sun
Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.
Lingling
L
Wang
Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Tao
T
Zhang
Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.
Zhi
Z
Zhou
Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Qiufen
Q
Jiang
Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Qilin
Q
Yi
Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.
Chuanyan
C
Yang
Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.
Limei
L
Qiu
Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. Electronic address: lshsong@qdio.ac.cn.
Linsheng
L
Song
D000818:Animals; D004156:Catechol Oxidase / Q000378:metabolism; D002478:Cells, Cultured; D049896:Crassostrea / Q000276:immunology / Q000378:metabolism*; D003542:Cystathionine gamma-Lyase / Q000378:metabolism; D004790:Enzyme Induction / Q000276:immunology; D004792:Enzyme Precursors / Q000378:metabolism; D004926:Escherichia coli / Q000276:immunology; D006434:Hemocytes / Q000201:enzymology / Q000276:immunology; D006862:Hydrogen Sulfide / Q000378:metabolism*; D056747:Immunomodulation; D008070:Lipopolysaccharides / Q000494:pharmacology; D010587:Phagocytosis; D013482:Superoxide Dismutase / Q000378:metabolism
D004792:Enzyme Precursors; D008070:Lipopolysaccharides; C038451:pro-phenoloxidase; D004156:Catechol Oxidase; D013482:Superoxide Dismutase; D003542:Cystathionine gamma-Lyase; D006862:Hydrogen Sulfide
Crassostrea gigas; Cystathionine γ lyase (CSE); Hydrogen sulfide (H(2)S); Immunity; LPS; Propargylglycine (PAG)