Streptococcus suis serotyping by a new multiplex PCR.
A multiplex PCR was developed to detect all true serotypes of Streptococcus suis. This multiplex PCR was composed of four reaction sets. The first set identified nine serotypes (serotypes 1/2, 1, 2, 3, 7, 9, 11, 14 and 16), the second set identified eight serotypes (serotypes 4, 5, 8, 12, 18, 19, 24 and 25), the third set identified seven serotypes (serotypes 6, 10, 13, 15, 17, 23 and 31), and the last set identified five serotypes (serotypes 21, 27, 28, 29 and 30). This assay correctly detected serotypes 2, 5, 14 and 24 in human isolates, and serotypes 1, 2, 1/2, 3, 4, 5, 6, 7, 8, 9, 11, 12, 14, 15, 16, 17, 19, 24, 28 and 31 in pig isolates from Thailand. No cross-reaction was observed with other bacterial species. Our multiplex PCR was able to simultaneously amplify a DNA mixture of reference Streptococcus suis serotypes. This assay should be useful for serotype surveillance of human and pig isolates of Streptococcus suis.
National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand.
Anusak
A
Kerdsin
Laboratory of Clinical Research on Infectious Diseases, International Research Center for Infectious Diseases, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
Yukihiro
Y
Akeda
Faculty of Science and Engineering, Kasetsart University, Chalermphrakiat Sakon Nakhon Province Campus, Sakon Nakhon, Thailand.
Rujirat
R
Hatrongjit
National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand.
Unchaya
U
Detchawna
Research Center for Food Safety, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
Tsutomu
T
Sekizaki
Thailand-Japan Research Collaboration Center for Emerging and Re-emerging Infections, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
Shigeyuki
S
Hamada
Faculty of Veterinary Medicine, University of Montreal, Québec, Canada.
Marcelo
M
Gottschalk
Infectious Disease Surveillance Center, National Institute of Infectious Diseases, Tokyo, Japan.|Laboratory of Clinical Research on Infectious Diseases, International Research Center for Infectious Diseases, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
Kazunori
K
Oishi
D000818:Animals; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012703:Serotyping / Q000379:methods / Q000662:veterinary*; D013290:Streptococcal Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary; D017011:Streptococcus suis / Q000145:classification* / Q000235:genetics*; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology; D013785:Thailand / Q000453:epidemiology