In vitro assessment of antineoplastic effects of deuterium depleted water.
BACKGROUND
In vitro, in vivo and clinical studies have demonstrated anti-cancer effects of deuterium depleted water (DDW). The nature of this agents action, cytotoxic or cytostatic, remains to be elucidated. We here aimed to address the point by examining effects on different cell lines.


METHODS
3-(4, 5-dimethylthiazol- 2-yl)-2, 5-diphenyltetrazolium bromide (MTT) -based cytotoxicity analysis was conducted for human breast, stomach, colon, prostate cancer and glioblastoma multiforme cell lines as well as human dermal fibroblasts. The cell lines were treated with decreasing deuterium concentrations of DDW alone, paclitaxel alone and both. One way analysis of variance (ANOVA) was used for statistical analysis.


RESULTS
Treatment with different deuterium concentrations of DDW alone did not impose any significant inhibitory effects on growth of cell lines. Paclitaxel significantly decreased the survival fractions of all cell lines. DDW augmented paclitaxel inhibitory effects on breast, prostate, stomach cancer and glioblastoma cell lines, with influence being more pronounced in breast and prostate cases.


CONCLUSIONS
DDW per se does not appear to have inhibitory effects on the assessed tumor cell lines as well as normal fibroblasts. As an adjuvant, however, DDW augmented inhibitory effects of paclitaxel and thus it could be considered as an adjuvant to conventional anticancer agents in future trials.
Cancer Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, Iran E-mail : smuhammadnejad@gmail.com.
Saeed
S
Soleyman-Jahi
Kazem
K
Zendehdel
Kambiz
K
Akbarzadeh
Mahnaz
M
Haddadi
Saeid
S
Amanpour
Samad
S
Muhammadnejad
D000970:Antineoplastic Agents / Q000494:pharmacology*; D002460:Cell Line; D045744:Cell Line, Tumor; D003903:Deuterium / Q000494:pharmacology*; D045325:HCT116 Cells; D006801:Humans; D017239:Paclitaxel / Q000494:pharmacology; D014867:Water / Q000494:pharmacology*
D000970:Antineoplastic Agents; D014867:Water; D003903:Deuterium; D017239:Paclitaxel