Evaluation of femoral perfusion in a rabbit model of steroid-induced osteonecrosis by dynamic contrast-enhanced MRI with a high magnetic field MRI system.
OBJECTIVE
To evaluate perfusion during the early phase after steroid administration in vivo using dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) with a high magnetic field MRI system. The main pathogenesis of steroid-induced osteonecrosis is considered to be ischemia.


METHODS
A single dose of methylprednisolone (MPSL) was injected into nine rabbits. DCE-MRI was performed for these rabbits before MPSL administration and 1, 5, 10, and 14 days after administration. Time-signal intensity curves were created for each femur based on the signal intensity to evaluate perfusion. Enhancement ratio (ER), initial slope (IS), and area under the curve (AUC) were calculated and the value before MPSL administration and the minimal value after administration were compared statistically.


RESULTS
ER, IS, and AUC values after MPSL administration significantly decreased (P < 0.05, P < 0.01, and P < 0.01, respectively). All of them decreased by the 5th day in 56% of the femora and by the 14th day in 83%, and some femora even showed a decrease from the 1st day.


CONCLUSIONS
In this study, decreased perfusion in the femora after steroid administration was proven. Additionally, we could show that it occurred from the early days after steroid administration.
Department of Orthopaedics, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto, Japan.
Shigeki
S
Hayashi
Mikihiro
M
Fujioka
Kazuya
K
Ikoma
Masazumi
M
Saito
Keiichiro
K
Ueshima
Masashi
M
Ishida
Masaaki
M
Kuribayashi
Akira
A
Ikegami
Osam
O
Mazda
Toshikazu
T
Kubo
D000465:Algorithms; D000818:Animals; D001783:Blood Flow Velocity; D003287:Contrast Media; D005263:Femoral Artery / Q000503:physiopathology*; D007089:Image Enhancement / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000379:methods; D018810:Magnetic Resonance Angiography / Q000379:methods*; D008297:Male; D008775:Methylprednisolone; D010020:Osteonecrosis / Q000139:chemically induced* / Q000473:pathology / Q000503:physiopathology*; D011817:Rabbits; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013256:Steroids
D003287:Contrast Media; D013256:Steroids; D008775:Methylprednisolone
dynamic contrast-enhanced MRI; evaluation of femoral perfusion; high magnetic field MRI system; steroid-induced osteonecrosis