Bone marrow-origin stem/progenitor cells in the mammary gland of heifers.
The aim of the study was to estimate the size of bone marrow-origin stem/progenitor population in 2-year old nonpregnant Holstein-Friesian heifers. Quantitative and qualitative analysis was done using scanning cytometry and confocal microscopy of mammary tissue slices labelled with the combination of two markers: Sca-1 (marker of stem-progenitor cells) and CD45 (marker of hematopoietic cells). The average (+/- SEM) percentage of Sca-1POS CD45 POS cells was 0.89 +/- 0.21. They were localized mainly outside of mammary ducts, in the stroma and sometimes intraluminally. Our results indicate that the subpopulation of Sca-1POS cells bearing CD45 antigen may enrich the niche of mammary stem/progenitor cells from the bone marrow and participate in the growth of the mammary gland in post-pubertal heifers.
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Osińska
M M
MM
Godlewski
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M
Wierzbicki
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T
Motyl
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002417:Cattle / Q000502:physiology*; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology; D047569:Laser Scanning Cytometry; D008321:Mammary Glands, Animal / Q000166:cytology*; D018613:Microscopy, Confocal; D013234:Stem Cells / Q000166:cytology*