Blue light-induced proteomic changes in etiolated Arabidopsis seedlings.
Plants adapt to environmental light conditions by photoreceptor-mediated physiological responses, but the mechanism by which photoreceptors perceive and transduce the signals is still unresolved. Here, we used 2D difference gel electrophoresis (2D DIGE) and mass spectrometry to characterize early molecular events induced by short blue light exposures in etiolated Arabidopsis seedlings. We observed the phosphorylation of phototropin 1 (phot1) and accumulation of weak chloroplast movement under blue light 1 (WEB1) in the membrane fraction after blue light irradiation. Over 50 spots could be observed for the two rows of phot1 spots in the 2-DE gels, and eight novel phosphorylated Ser/Thr sites were identified in the N-terminus and Hinge 1 regions of phot1 in vivo. Blue light caused ubiquitination of phot1, and K526 of phot1 was identified as a putative ubiquitination site. Our study indicates that post-translational modification of phot1 is more complex than previously reported.
Department of Plant Biology, Carnegie Institution for Science , Stanford, California 94305, United States.
Zhiping
Z
Deng
Juan A
JA
Oses-Prieto
Ulrich
U
Kutschera
Tong-Seung
TS
Tseng
Lingzhao
L
Hao
Alma L
AL
Burlingame
Zhi-Yong
ZY
Wang
Winslow R
WR
Briggs
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites / Q000235:genetics; D015153:Blotting, Western; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D002853:Chromatography, Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D064527:Etiolation / Q000528:radiation effects; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D008027:Light; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010748:Phosphopeptides / Q000235:genetics / Q000378:metabolism; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism; D010766:Phosphorylation / Q000528:radiation effects; D017346:Protein Serine-Threonine Kinases; D020543:Proteome / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D036226:Seedlings / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects*; D017386:Sequence Homology, Amino Acid; D012694:Serine / Q000378:metabolism; D013912:Threonine / Q000378:metabolism
D029681:Arabidopsis Proteins; D002352:Carrier Proteins; D010748:Phosphopeptides; D010750:Phosphoproteins; D020543:Proteome; C554340:Web1 protein, Arabidopsis; D049452:Green Fluorescent Proteins; D013912:Threonine; D012694:Serine; C103646:NPH1 protein, Arabidopsis; D017346:Protein Serine-Threonine Kinases