Stress mitigating and growth enhancing effect of dietary tryptophan in rohu (Labeo rohita, Hamilton, 1822) fingerlings.
An experiment was conducted to study the stress mitigation and growth enhancing role of dietary L-tryptophan (TRP) under thermal stress in rohu, Labeo rohita fingerlings for 45 days. Seven hundred and twenty fishes were distributed in three major groups that are ambient temperature (26 °C), 34 and 38 °C in triplicate following a complete randomized design. Acclimation of fishes to 34 and 38 °C over average ambient temperatures were carried out at 1 °C/day. Each group was fed with a diet supplemented with 0, 0.36, 0.72 or 1.42 % L-TRP. Results showed that blood glucose and serum cortisol level were found to be significantly higher (p < 0.05) in the higher temperature groups than the ambient temperature group. Similarly, aminotransferase, lactate dehydrogenase, malate dehydrogenase, CAT, superoxide dismutase activities were found to be significantly higher (p < 0.05) in the control groups (0 % L-TRP) and decreasing activities of these enzymes were observed with the increasing level of dietary L-TRP. In different temperature groups, L-TRP-supplemented groups were found to have higher (p < 0.05) growth, RGR and PER. The results obtained in the present study indicate that dietary L-TRP mitigates thermal stress and enhances growth. From the present study, we can conclude that dietary supplementation of L-TRP at the 0.72 % level in the diet is found to be optimum to reduce thermal stress even up to 38 °C in rohu, L. rohita. The baseline data obtained here could be useful for the farmers to formulate feeds to culture the fish in different agro-climatic zones.
Central Institute of Fisheries Education, Yari Road, Versova, Mumbai, 400061, India.
Prem
P
Kumar
Shailesh
S
Saurabh
A K
AK
Pal
N P
NP
Sahu
A R T
AR
Arasu
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D003530:Cyprinidae / Q000254:growth & development*; D019587:Dietary Supplements; D006854:Hydrocortisone / Q000097:blood; D007194:India; D010088:Oxidoreductases / Q000097:blood; D011863:Radioimmunoassay / Q000662:veterinary; D013312:Stress, Physiological / Q000187:drug effects*; D013696:Temperature; D014364:Tryptophan / Q000494:pharmacology*; D015430:Weight Gain / Q000187:drug effects
D001786:Blood Glucose; D014364:Tryptophan; D010088:Oxidoreductases; D006854:Hydrocortisone