Organic extracts of coke oven emissions can induce genetic damage in metabolically competent HepG2 cells.
Coke oven emissions (COEs) containing various carcinogenic polycyclic aromatic hydrocarbons (PAHs) represent the coal-burning pollution in the air. Organic pollutants in the aerosol and particulate matter of COEs were collected from the bottom, side, and top of a coke oven. The Comet assay and cytokinesis-block micronucleus cytome assay were conducted to analyze the genetic damage of extractable organic matter (EOM) of COEs on HepG2 cells. All the three EOMs could induce significant dose-dependent increases in Olive tail moment, tail DNA, and tail length, micronuclei, nucleoplasmic bridges, and nuclear buds frequencies, which were mostly positively correlated with the total PAHs concentration in each EOM. In conclusion, EOMs of COEs in the three typical working places of coke oven can induce DNA strand breaks and genomic instability in the metabolically competent HepG2 cells. The PAHs in EOMs may be important causative agents for the genotoxic effects of COEs.
Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, Hubei, China; Institute of Occupational and Environmental Health, School of Public Health, Medical College of Soochow University, 199 Renai Road, Suzhou 215123, Jiangsu, China.
Lili
L
Xin
KunShan Health Inspection Station, 458 Tongfeng Road, Kunshan 215301, Jiangsu, China.
Jianshu
J
Wang
Institute of Occupational and Environmental Health, School of Public Health, Medical College of Soochow University, 199 Renai Road, Suzhou 215123, Jiangsu, China.
Sifan
S
Guo
Institute of Occupational and Environmental Health, School of Public Health, Medical College of Soochow University, 199 Renai Road, Suzhou 215123, Jiangsu, China.
Yanhu
Y
Wu
Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, Hubei, China.
Xiaohai
X
Li
Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, Hubei, China.
Huaxin
H
Deng
Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, Hubei, China.
Dan
D
Kuang
Institute of Occupational and Environmental Health, School of Public Health, Medical College of Soochow University, 199 Renai Road, Suzhou 215123, Jiangsu, China.
Wei
W
Xiao
Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, Hubei, China.
Tangchun
T
Wu
Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, Hubei, China. Electronic address: sophie5011@163.com.
Huan
H
Guo
D000393:Air Pollutants / Q000032:analysis / Q000633:toxicity*; D002470:Cell Survival / Q000187:drug effects; D003077:Coke; D020552:Comet Assay; D045424:Complex Mixtures / Q000032:analysis / Q000633:toxicity*; D004249:DNA Damage; D056945:Hep G2 Cells; D006801:Humans; D015162:Micronucleus Tests; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis / Q000633:toxicity*
D000393:Air Pollutants; D003077:Coke; D045424:Complex Mixtures; D011084:Polycyclic Aromatic Hydrocarbons
Coke oven emissions; Comet assay; Cytokinesis-block micronucleus cytome assay; Extractable organic matter; Genotoxicity; Polycyclic aromatic hydrocarbon