In Vivo Detection of Diffuse Inflammation in Secondary Progressive Multiple Sclerosis Using PET Imaging and the Radioligand ¹¹C-PK11195.
Patients with secondary progressive multiple sclerosis (SPMS) are lacking efficient medication to slow down the progression of their disease. PET imaging holds promise as a method to study, at the molecular level and in vivo, the central nervous system pathology of SPMS. PET might thus help to elucidate potential therapeutic targets and be useful as an imaging biomarker in future treatment trials of progressive multiple sclerosis. The objective of this study was to evaluate whether translocator protein (TSPO) imaging could be used to visualize the diffuse inflammation located in the periplaque area and in the normal-appearing white matter (NAWM) in the brains of patients with SPMS.


METHODS
This was an imaging study using MR imaging and PET with (11)C-PK11195 binding to TSPO, which is expressed in activated, but not in resting, microglia. Ten SPMS patients with a mean expanded disability status scale score of 6.3 (SD, 1.5) and eight age-matched healthy controls were studied. The imaging was performed using High-Resolution Research Tomograph PET and 1.5-T MR imaging scanners. Microglial activation was evaluated as the distribution volume ratio (DVR) of (11)C-PK11195 from dynamic PET images. DVR estimations were performed with special interest in NAWM and gray matter using region-of-interest and parametric image-based approaches.


RESULTS
The DVR of (11)C-PK11195 was significantly increased in the periventricular and total NAWM (P = 0.016 and P < 0.001, respectively) and in the thalamic ROIs (P = 0.027) of SPMS patients, compared with the control group. Similarly, parametric image analysis showed widespread increases of (11)C-PK11195 in the white matter of SPMS patients, compared with healthy controls. Increased perilesional TSPO uptake was present in 57% of the chronic T1 lesions in MR imaging.


CONCLUSIONS
The finding of increased (11)C-PK11195 binding in the NAWM of SPMS patients is in line with the neuropathologic demonstration that activated microglial cells are the source of diffuse NAWM inflammation. Evaluating microglial activation with TSPO-binding PET ligands provides a unique tool to assess diffuse brain inflammation and perilesional activity in progressive multiple sclerosis in vivo.
Turku PET Centre, Turku University Hospital and University of Turku, Turku, Finland Division of Clinical Neurosciences, Turku University Hospital and University of Turku, Turku, Finland eerris@utu.fi.
Eero
E
Rissanen
Turku PET Centre, Turku University Hospital and University of Turku, Turku, Finland.
Jouni
J
Tuisku
Turku PET Centre, Turku University Hospital and University of Turku, Turku, Finland.
Johanna
J
Rokka
Medical Imaging Centre of Southwest Finland, Turku University Hospital and University of Turku, Turku, Finland; and.
Teemu
T
Paavilainen
Turku PET Centre, Turku University Hospital and University of Turku, Turku, Finland Department of Radiology, University Hospital of Tampere, Tampere, Finland.
Riitta
R
Parkkola
Turku PET Centre, Turku University Hospital and University of Turku, Turku, Finland.
Juha O
JO
Rinne
Division of Clinical Neurosciences, Turku University Hospital and University of Turku, Turku, Finland.
Laura
L
Airas
D000328:Adult; D000368:Aged; D001921:Brain / Q000000981:diagnostic imaging / Q000378:metabolism; D002250:Carbon Radioisotopes; D016022:Case-Control Studies; D018450:Disease Progression; D005260:Female; D006801:Humans; D007546:Isoquinolines / Q000378:metabolism*; D008024:Ligands; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000000981:diagnostic imaging* / Q000378:metabolism; D049268:Positron-Emission Tomography; D018079:Receptors, GABA / Q000378:metabolism
D002250:Carbon Radioisotopes; D007546:Isoquinolines; D008024:Ligands; D018079:Receptors, GABA; C499229:TSPO protein, human; C037850:PK 11195
PET; disease progression; microglia; multiple sclerosis