Interaction of curcumin with manganese may compromise metal and neurotransmitter homeostasis in the hippocampus of young mice.
Manganese (Mn) exposure is related to industrial activities, where absorption by inhalation has high relevance. Manganism, a syndrome caused as a result of excessive accumulation of Mn in the central nervous system, has numerous symptoms similar to those seen in idiopathic Parkinson disease (IPD). Some of these symptoms, such as learning, memory, sensorial, and neurochemical changes, appear before the onset of motor deficits in both manganism and IPD. The aim of this study was to evaluate the possible neuroprotective effects of curcumin against behavioral deficits induced by Mn toxicity in young (2 months old) Swiss mice. We evaluated the effect of chronic inhalation of a Mn mixture [Mn(OAc)3 and MnCl2 (20:40 mM)], 1 h/session, three times a week, over a 14-week period on behavioral and neurochemical parameters. Curcumin was supplemented in the diet (500 or 1,500 ppm in food pellets). The Mn disrupted the motor performance evaluated in the single-pellet reach task, as well as the short- and long-term spatial memory evaluated in the step-down inhibitory avoidance task. Surprisingly, curcumin also produced similar deleterious effects in such behavioral tests. Moreover, the association of Mn plus curcumin significantly increased the levels of Mn and iron, and decreased the levels of dopamine and serotonin in the hippocampus. These alterations were not observed in the striatum. In conclusion, the current Mn treatment protocol resulted in mild deficits in motor and memory functions, resembling the early phases of IPD. Additionally, curcumin showed no beneficial effects against Mn-induced disruption of hippocampal metal and neurotransmitter homeostasis.
Department of Biochemistry, Federal University of Santa Catarina, Biological Sciences Centre, 88040-900, Florianópolis, SC, Brazil.
Ariana Ern
AE
Schmitz
Paulo Alexandre
PA
de Oliveira
Luiz F
LF
de Souza
Danilo Grünig Humberto
DG
da Silva
Samara
S
Danielski
Danúbia Bonfanti
DB
Santos
Eduardo Alves
EA
de Almeida
Rui Daniel
RD
Prediger
Andrew
A
Fisher
Marcelo
M
Farina
Alcir Luiz
AL
Dafre
D000085:Acetates / Q000008:administration & dosage / Q000494:pharmacology; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000494:pharmacology; D002712:Chlorides / Q000008:administration & dosage / Q000494:pharmacology; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism; D003474:Curcumin / Q000008:administration & dosage / Q000494:pharmacology*; D004298:Dopamine / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism; D007501:Iron / Q000378:metabolism; D008297:Male; D008345:Manganese / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology*; D017895:Manganese Compounds / Q000008:administration & dosage / Q000494:pharmacology; D008568:Memory / Q000187:drug effects; D008670:Metals / Q000378:metabolism*; D051379:Mice; D009043:Motor Activity / Q000187:drug effects; D018377:Neurotransmitter Agents / Q000378:metabolism*; D012701:Serotonin / Q000378:metabolism
D000085:Acetates; D000894:Anti-Inflammatory Agents, Non-Steroidal; D002712:Chlorides; D017895:Manganese Compounds; D008670:Metals; D018377:Neurotransmitter Agents; D012701:Serotonin; D008345:Manganese; D007501:Iron; D003474:Curcumin; C025340:manganese chloride; D004298:Dopamine