In vivo study of chitosan-natural nano hydroxyapatite scaffolds for bone tissue regeneration.
Significant development has been achieved with bioceramics and biopolymer scaffolds in the construction of artificial bone. In the present study, we have developed and compared chitosan-micro hydroxyapatite (chitosan-mHA) and chitosan-nano hydroxyapatite (chitosan-nHA) scaffolds as bone graft substitutes. The biocompatibility and cell proliferation of the prepared scaffolds were checked with preosteoblast (MC3T3-E1) cells. Total Volume (TV), bone volume (BV), bone surface (BS), trabecular thickness (Tb.Th), trabecular number (Tb.N) and trabecular separation (Tb.Sp) were found to be higher in chitosan-nHA than chitosan-mHA scaffold. Hence, we suggest that chitosan-nHA scaffold could be a promising biomaterial for bone tissue engineering.
Department of Orthopaedic Surgery, Pusan National University Hospital, Busan 602-739, Republic of Korea.
Jong Seo
JS
Lee
Department of Medicine, Graduate School, Pusan National University, Busan 602-739, Republic of Korea.
Sang Dae
SD
Baek
Department of Marine Bio Convergence Science and Marine Bioprocess Research Center, Pukyong National University, Busan 608-737, Republic of Korea. Electronic address: venkatjchem@pknu.ac.kr.
Jayachandran
J
Venkatesan
Nanotheranostics Laboratory, Centre for Cellular and Molecular Biology, Hyderabad 500-007, India.
Ira
I
Bhatnagar
Department of Pathology, Medical College, Kosin University, Busan 602-739, Republic of Korea.
Hee Kyung
HK
Chang
Department of Orthopaedic Surgery, Pusan National University Hospital, Busan 602-739, Republic of Korea. Electronic address: kimht@pusan.ac.kr.
Hui Taek
HT
Kim
Department of Marine Bio Convergence Science and Marine Bioprocess Research Center, Pukyong National University, Busan 608-737, Republic of Korea. Electronic address: sknkim@pknu.ac.kr.
Se-Kwon
SK
Kim
D000818:Animals; D001861:Bone Regeneration; D001842:Bone and Bones / Q000187:drug effects / Q000293:injuries; D049109:Cell Proliferation / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry* / Q000627:therapeutic use; D017886:Durapatite / Q000737:chemistry / Q000627:therapeutic use; D006801:Humans; D051379:Mice; D011817:Rabbits; D023822:Tissue Engineering; D054457:Tissue Scaffolds
D048271:Chitosan; D017886:Durapatite
Chitosan; Marine biomaterials; Natural hydroxyapatite