Vasorelaxing activity of two coumarins from Murraya paniculata leaves.
In the search for novel chemical scaffolds leading to potential antihypertensive agents, the methanol extract of Murraya paniculata leaves was assessed for its effects on isolated rat aorta rings. The vasorelaxing effect of the chloroform fraction of the methanol plant extract was the most potent for its vasorelaxing activity on rat aorta rings contracted by 60 mM K(+) (K60). Two coumarins were isolated from the chloroform fraction: the novel kimcuongin (1) and the known murracarpin (2). Their structures were determined from spectroscopic evidences including (1)H- and (13)C-NMR, correlation spectroscopy (COSY), nuclear Overhauser effect spectroscopy (NOESY), heteronuclear multiple bond correlation (HMBC), heteronuclear single quantum correlation (HSQC), and high resolution mass spectrometry (HR-MS). Kimcuongin and, to a lesser extent, murracarpin, showed vasorelaxing activity with IC50 values of 37.7 µM and 139.3 µM, respectively. The coumarins kimcuongin and murracarpin may thus represent a novel class of vasodilators of natural source.
Department of Bioactive Products, Institute of Natural Products Chemistry, Vietnam Academy of Science and Technology (VAST).
Nguyen Manh
NM
Cuong
Pham Ngoc
PN
Khanh
Ho Viet
HV
Duc
Tran Thu
TT
Huong
Bui Huu
BH
Tai
Nguyen Quoc
NQ
Binh
Miriam
M
Durante
Fabio
F
Fusi
D000818:Animals; D001011:Aorta / Q000187:drug effects / Q000502:physiology; D003374:Coumarins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D066298:In Vitro Techniques; D032101:Murraya / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D051381:Rats; D014664:Vasodilation / Q000187:drug effects*; D014665:Vasodilator Agents / Q000494:pharmacology*
C000598345:2H-8-(2-hydroxy-1-methoxy-3-methyl-3-buten-1-yl)-7-methoxy-1-benzopyran-2-one; C000598344:2H-8-(2-senecioyloxy-3-methylbut-2-enoyl)-7-methoxy-1-benzopyran-2-one; D003374:Coumarins; D010936:Plant Extracts; D014665:Vasodilator Agents