[Inhibition of the activity of sulfate-reducing bacteria in produced water from oil reservoir by nitrate].
Growth and metabolic activity of sulfate-reducing bacteria (SRB) can result in souring of oil reservoirs, leading to various problems in aspects of environmental pollution and corrosion. Nitrate addition and management of nitrate-reducing bacteria (NRB) offer potential solutions to controlling souring in oil reservoirs. In this paper, a facultive chemolithotrophic NRB, designated as DNB-8, was isolated from the produced fluid of a water-flooded oil reservoir at Daqing oilfield. Then the efficacies and mechanisms of various concentrations of nitrate in combination with DNB-8 in the inhibition of the activity of SRB enriched culture were compared. Results showed that 1.0 mmol x L(-1) of nitrate or 0.45 mmol x L(-1) of nitrite inhibited the sulfate-reducing activity of SRB enrichments; the competitive reduction of nitrate by DNB-8 and the nitrite produced were responsible for the suppression. Besides, the SRB enrichment cultures showed a metabolic pathway of dissimilatory nitrate reduction to ammonium (DNRA) via nitrite. The SRB cultures could possibly alleviate the nitrite inhibition by DNRA when they were subjected to high-strength nitrate.
De-Yu
DY
Yang
Ying
Y
Zhang
Rong-Jiu
RJ
Shi
Si-Qin
SQ
Han
Guang-Zhe
GZ
Li
Guo-Qiao
GQ
Li
Jin-Yi
JY
Zhao
D003343:Corrosion; D009566:Nitrates / Q000737:chemistry*; D009573:Nitrites / Q000737:chemistry; D061329:Oil and Gas Fields / Q000382:microbiology*; D016999:Sulfur-Reducing Bacteria / Q000187:drug effects / Q000378:metabolism*; D014867:Water
D009566:Nitrates; D009573:Nitrites; D014867:Water