Epoxyeicosatrienoic acids attenuating hypotonic-induced apoptosis of IMCD cells via γ-ENaC inhibition.
Inner medulla collecting duct (IMCD) cells are the key part for urinary concentration. Hypotonic stress may trigger apoptosis of IMCD cells and induce renal injury. Epoxyeicosatrienoic acids (EETs) play an important role in anti-apoptosis, but their roles in hypotonic-induced apoptosis of IMCD cells are still unclear. Here we found increasing exogenous 11, 12-EET or endogenous EETs with Ad-CMV-CYP2C23-EGFP transfection decreased apoptosis of IMCD cells induced by hypotonic stress. Moreover, up-regulation of γ-ENaC induced by hypotonic stress was abolished by elevation of exogenous or endogenous EETs. Collectively, this study illustrated that EETs attenuated hypotonic-induced apoptosis of IMCD cells, and that regulation of γ-ENAC may be a possible mechanism contributing to the anti-apoptotic effect of EETs in response to hypotonic stress.
Guangdong Province Key Laboratory of Arrhythmia and Electrophysiology, Guangzhou, China; Department of Critical Care Medicine, Sun Yat-sen Memorial Hospital of Sun Yat-sen University, Guangzhou, China.
Luyun
L
Wang
Guangdong Province Key Laboratory of Arrhythmia and Electrophysiology, Guangzhou, China; Department of Cardiology, Sun Yat-sen Memorial Hospital of Sun Yat-sen University, Guangzhou, China.
Yang
Y
Liu
Zhongshan City Hospital of Chinese Medicine,Affiliated Hospital of Guangzhou University of Chinese Medicine, Zhongshan, China.
Huamin
H
Wang
Division of Nephrology, Department of Internal Medicine, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
Xun
X
Liu
Guangdong Province Key Laboratory of Arrhythmia and Electrophysiology, Guangzhou, China; Radiotherapy Department, Sun Yat-sen Memorial Hospital of Sun Yat-sen University, Guangzhou, China.
Jie
J
Chen
Department of Physiology, Georgia Regents University, Augusta, Georgia, United States of America.
Mong-Heng
MH
Wang
Guangdong Province Key Laboratory of Arrhythmia and Electrophysiology, Guangzhou, China; Department of Cardiology, Sun Yat-sen Memorial Hospital of Sun Yat-sen University, Guangzhou, China.
Jingfeng
J
Wang
Guangdong Province Key Laboratory of Arrhythmia and Electrophysiology, Guangzhou, China; Department of Cardiology, Sun Yat-sen Memorial Hospital of Sun Yat-sen University, Guangzhou, China.
Hui
H
Huang
D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D000090762:Cytochrome P-450 CYP2J2; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism; D015777:Eicosanoids / Q000494:pharmacology*; D053503:Epithelial Sodium Channels / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D005786:Gene Expression Regulation / Q000187:drug effects; D005822:Genetic Vectors / Q000235:genetics; D007685:Kidney Tubules, Collecting / Q000166:cytology* / Q000378:metabolism*; D008297:Male; D009997:Osmotic Pressure; D051381:Rats; D014161:Transduction, Genetic
C000717351:Cyp2c23 protein, rat; D015777:Eicosanoids; D053503:Epithelial Sodium Channels; D003577:Cytochrome P-450 Enzyme System; D000090762:Cytochrome P-450 CYP2J2