Methyl salicylate lactoside inhibits inflammatory response of fibroblast-like synoviocytes and joint destruction in collagen-induced arthritis in mice.
OBJECTIVE
Methyl salicylate 2-O-β-d-lactoside (MSL), whose chemical structure is similar to that of salicylic acid, is a natural product derivative isolated from a traditional Chinese herb. The aim of this study was to investigate the therapeutic effect of MSL in mice with collagen-induced arthritis (CIA) and explore its underlying mechanism.


METHODS
The anti-arthritic effects of MSL were evaluated on human rheumatoid fibroblast-like synoviocytes (FLS) in vitro and CIA in mice in vivo by obtaining clinical scores, measuring hind paw thickness and inflammatory cytokine levels, radiographic evaluations and histopathological assessments.


RESULTS
Treatment with MSL after the onset of arthritis significantly prevented the progression and development of rheumatoid arthritis (RA) in CIA mice without megascopic gastric mucosa damage. In addition, MSL inhibited the production of pro-inflammatory mediators, the phosphorylation and translocation of NF-κB, and cell proliferation induced by TNF-α in FLS. MSL non-selectively inhibited the activity of COX in vitro, but was a more potent inhibitor of COX-2 than COX-1. MSL also inhibited the phosphorylation of inhibitor of NF-κB kinase, IκBα and p65, thus blocking the nuclear translocation of NF-κB in TNF-α-stimulated FLS.


CONCLUSIONS
MSL exerts therapeutic effects on CIA mice, suppressing the inflammatory response and joint destruction by non-selectively inhibiting the activity of COX and suppressing activation of the NF-κB signalling pathway, but without damaging the gastric mucosa. Therefore, MSL has great potential to be developed into a novel therapeutic agent for the treatment of RA.
State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China; Key Laboratory of Molecular Pharmacology and Drug Evaluation (Ministry of Education of China), School of Pharmacy, Yantai University, Yantai, China; Binzhou Medical University, Yantai, China.
Wenyu
W
Xin
Chao
C
Huang
Xue
X
Zhang
Sheng
S
Xin
Yiming
Y
Zhou
Xiaowei
X
Ma
Dan
D
Zhang
Yongjie
Y
Li
Sibai
S
Zhou
Dongming
D
Zhang
Tiantai
T
Zhang
Guanhua
G
Du
D000818:Animals; D000843:Ankle Joint / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology*; D018501:Antirheumatic Agents / Q000737:chemistry / Q000494:pharmacology*; D001169:Arthritis, Experimental / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D049109:Cell Proliferation / Q000187:drug effects; D003094:Collagen; D006801:Humans; D007249:Inflammation / Q000188:drug therapy / Q000378:metabolism; D018836:Inflammation Mediators / Q000037:antagonists & inhibitors / Q000378:metabolism; D007785:Lactose / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D051381:Rats; D017208:Rats, Wistar; D012459:Salicylates / Q000737:chemistry / Q000494:pharmacology*; D013583:Synovial Membrane / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology*; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors / Q000378:metabolism
D018501:Antirheumatic Agents; D018836:Inflammation Mediators; D012459:Salicylates; D014409:Tumor Necrosis Factor-alpha; C000597005:methyl salicylate 2-O-lactoside; D003094:Collagen; D007785:Lactose
CIA; NF-κB signal pathway; cyclooxygenase; fibroblast-like synoviocytes; methyl salicylate 2-O-β-d-lactoside; pro-inflammatory cytokine; rheumatoid arthritis