Heterotopic ossification in metastatic colorectal carcinoma: case report with morphoproteomic insights into the histogenesis.
A case of metastatic colorectal carcinoma with heterotopic ossification is described. Objective Morphoproteomic analysis was performed to help define the histogenesis of the heterotopic ossification in this context. Design Immunohistochemical stains for Gli2, α-SMA, SPARC (osteonectin), and nestin were performed and the expression level (chromogenic signal intensity) and subcellular compartmentalization of these protein analytes were assessed in the tumor cells vis-à-vis the companionate stromal cells and osteoblasts in a morphoproteomic application. Results This analysis revealed that the heterotopic ossification is more likely the result of pluripotent stromal cells that undergo differentiation to form osteoblasts rather than the tumor cells undergoing osseous metaplasia. Conclusion Morphoproteomics provides evidence that the histogenesis of heterotopic ossification in this case of metastatic colon cancer is from the stromal cells in the tumoral microenvironment.
M.D.; UT Health-Medical School at Houston, 6431 Fannin Street MSB 2.260B, Houston, TX 77030; phone: 1-713 500 5362; fax: 1-713 500 0695; e mail: jamie.buryanek@uth.tmc.edu.
Richard Sheng Poe
RS
Huang
Robert E
RE
Brown
Jamie
J
Buryanek
D000368:Aged; D015179:Colorectal Neoplasms / Q000150:complications / Q000378:metabolism / Q000473:pathology* / Q000556:secondary*; D006801:Humans; D007150:Immunohistochemistry; D051741:Kruppel-Like Transcription Factors / Q000378:metabolism; D008297:Male; D009687:Nuclear Proteins / Q000378:metabolism; D009999:Ossification, Heterotopic / Q000150:complications* / Q000378:metabolism* / Q000473:pathology; D010006:Osteoblasts / Q000473:pathology; D040901:Proteomics; D017154:Stromal Cells / Q000473:pathology; D000075746:Zinc Finger Protein Gli2
C082593:GLI2 protein, human; D051741:Kruppel-Like Transcription Factors; D009687:Nuclear Proteins; D000075746:Zinc Finger Protein Gli2
Heterotopic Ossification; and Morphoproteomics