Identification of lymphogranuloma venereum-associated Chlamydia trachomatis serovars by fluorescence in situ hybridisation--a proof-of-principle analysis.
We describe a proof-of-principle evaluation of a fluorescence in situ hybridisation (FISH) procedure to identify Chlamydia trachomatis serovars L1-L3, the causative agents of lymphogranuloma venereum, in cell cultures based on newly designed DNA probes. Rapid and easy-to-perform FISH could facilitate the diagnosis of lymphogranuloma venereum without nucleic acid amplification or serotyping, but requires broader evaluation studies, for example, in tropical high-endemicity regions.
Department of Tropical Medicine at the Bernhard Nocht Institute, German Armed Forces Hospital of Hamburg, Hamburg, Germany; Institute for Microbiology, Virology and Hygiene, University Hospital Rostock, Rostock, Germany.
Hagen
H
Frickmann
Andreas
A
Essig
Sven
S
Poppert
D002690:Chlamydia Infections / Q000175:diagnosis* / Q000382:microbiology; D002692:Chlamydia trachomatis / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008219:Lymphogranuloma Venereum / Q000175:diagnosis / Q000382:microbiology*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012703:Serotyping / Q000379:methods
Chlamydia trachomatis; cell culture; diagnostic microbiology; fluorescence in situ hybridisation; lymphogranuloma venereum