Decrease of Both Cofilin and LIM Kinase Phosphorylation in the Skeletal Muscles of Immobilization-induced Atrophy Rats.
[Purpose] Immobilization-induced atrophy is a general phenomenon caused by prolonged muscle disuse associated with orthopaedic conditions. However, changes in the phosphorylation of atrophy-related cofilin and LIM kinases are still poorly understood. In this study, we examined whether or not phosphorylation of cofilin and LIM kinases is altered in the skeletal muscles of rats after 3, 7, 14, and 21 days of cast immobilization. [Methods] We used two-dimensional gel electrophoresis, mass spectrometry, and western blotting to examine protein expression and phosphorylation in atrophied rat gastrocnemius muscles. [Results] The expression of the cofilin was detected in gastrocnemius muscle strips using proteomic analysis. Cast immobilization after 3, 7, 14, and 21 days significantly diminished the phosphorylation of cofilin and LIM kinases. [Conclusion] The present results suggest that cast immobilization-induced atrophy may be in part related to changes in the phosphorylation of cofilin and LIM kinases in rat skeletal muscles.
Laboratory of Health Science and Nanophysiotherapy, Department of Physical Therapy, Graduate School, Yongin University, Republic of Korea.
Mee-Young
MY
Kim
Laboratory of Health Science and Nanophysiotherapy, Department of Physical Therapy, Graduate School, Yongin University, Republic of Korea.
Ju-Hyun
JH
Kim
Laboratory of Health Science and Nanophysiotherapy, Department of Physical Therapy, Graduate School, Yongin University, Republic of Korea.
Jeong-Uk
JU
Lee
Laboratory of Health Science and Nanophysiotherapy, Department of Physical Therapy, Graduate School, Yongin University, Republic of Korea.
Lim-Kyu
LK
Lee
Laboratory of Health Science and Nanophysiotherapy, Department of Physical Therapy, Graduate School, Yongin University, Republic of Korea.
Seung-Min
SM
Yang
Laboratory of Health Science and Nanophysiotherapy, Department of Physical Therapy, Graduate School, Yongin University, Republic of Korea.
Hye-Joo
HJ
Jeon
Laboratory of Health Science and Nanophysiotherapy, Department of Physical Therapy, Graduate School, Yongin University, Republic of Korea.
Won-Deok
WD
Lee
Laboratory of Health Science and Nanophysiotherapy, Department of Physical Therapy, Graduate School, Yongin University, Republic of Korea.
Ji-Woong
JW
Noh
Department of Combative Martial Arts Training, College of Martial Arts, Yongin University, Republic of Korea.
Tae-Hyun
TH
Lee
Taekwondo Instructor Education, College of Martial Arts, Yongin University, Republic of Korea.
Taek-Yong
TY
Kwak
Institute of Functional Genomics, Department of Physiology, School of Medicine, Konkuk University, Republic of Korea.
Bokyung
B
Kim
Department of Physical Therapy, College of Public Health and Welfare, Yongin University, Republic of Korea.
Junghwan
J
Kim
Cofilin; LIM kinases; Skeletal muscle atrophy