Inhibition of inflammatory injure by polysaccharides from Bupleurum chinense through antagonizing P-selectin.
P-selectin-mediated adhesion between endothelium and neutrophils is a crucial process leading to acute inflammatory injure. Thus, P-selectin has been considered as promising target for therapeutics of acute inflammatory-related diseases. In the present study, the water-soluble polysaccharides (BCPs) were isolated from Bupleurum chinense, and we evaluated their therapeutical effects on acute inflammatory injure and antagonistic function against P-selectin-mediated neutrophil adhesion. Our results showed that BCPs significantly impaired the leukocyte infiltration and relieve lung injury in LPS-induced acute pneumonia model. BCPs significantly blocked the binding of P-selectin to neutrophils and inhibited P-selectin-mediated neutrophils rolling along CHO-P cell monolayer. The result from in vitro protein binding assay showed a direct evidence indicating that BCPs-treatment significantly eliminated the interaction between rhP-Fc and its physiological ligand PSGL-1 at protein level. Together, these results provide a novel therapeutical strategy for amelioration of inflammation-related disease processes by polysaccharides from B. chinense.
Life Science Research Center, Beihua University, Jilin 132013, China. Electronic address: tonghaibin@gmail.com.
Haibin
H
Tong
Life Science Research Center, Beihua University, Jilin 132013, China.
Dan
D
Tian
Jiangmen Entry-exit Inspection and Quarantine Bureau, Jiangmen 529000, China.
Tianbao
T
Li
College of Chemistry and Biology, Beihua University, Jilin 132013, China.
Bo
B
Wang
College of Forestry, Beihua University, Jilin 132013, China.
Guiquan
G
Jiang
Life Science Research Center, Beihua University, Jilin 132013, China.
Xin
X
Sun
D000818:Animals; D029972:Bupleurum; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D006801:Humans; D007249:Inflammation / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D019007:P-Selectin / Q000037:antagonists & inhibitors*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use
D019007:P-Selectin; D010936:Plant Extracts; D011134:Polysaccharides
Acute inflammation; Bupleurum chinense; Neutrophil adhesion; P-selectin; Polysaccharide