Comparison of commercial antimicrobial susceptibility test methods for testing of Staphylococcus aureus and Enterococci against vancomycin, daptomycin, and linezolid.
Three commercial antimicrobial susceptibility testing (AST) methods were compared to broth microdilution for testing of Staphylococcus aureus and enterococci against vancomycin, daptomycin, and linezolid. Despite high levels of categorical agreement and essential agreement, vancomycin MICs determined by MicroScan were often 1 log2 concentration higher and MICs determined by Phoenix 1 log2 concentration lower. Daptomycin MICs were 1 to 2 log2 concentrations higher by all AST methods, except Etest, potentially impacting definitive antimicrobial therapy for bloodstream infections due to these organisms.
The Johns Hopkins University, School of Medicine, Department of Pathology, Division of Microbiology, Baltimore, Maryland, USA Johns Hopkins Bayview Medical Center, Baltimore, Maryland, USA sriedel2@jhmi.edu.
Stefan
S
Riedel
The Johns Hopkins University, School of Medicine, Department of Pathology, Division of Microbiology, Baltimore, Maryland, USA.
Kar Mun
KM
Neoh
The Johns Hopkins University, School of Medicine, Department of Pathology, Division of Microbiology, Baltimore, Maryland, USA.
Stephen W
SW
Eisinger
Johns Hopkins Bayview Medical Center, Baltimore, Maryland, USA.
Lisa M
LM
Dam
The Johns Hopkins Hospital, Baltimore, Maryland, USA.
Tsigereda
T
Tekle
The Johns Hopkins University, School of Medicine, Department of Pathology, Division of Microbiology, Baltimore, Maryland, USA The Johns Hopkins Hospital, Baltimore, Maryland, USA.
Karen C
KC
Carroll
D000081:Acetamides / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D017576:Daptomycin / Q000494:pharmacology; D016983:Enterococcus / Q000187:drug effects*; D006801:Humans; D000069349:Linezolid; D008826:Microbial Sensitivity Tests / Q000379:methods*; D023303:Oxazolidinones / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000187:drug effects*; D014640:Vancomycin / Q000494:pharmacology
D000081:Acetamides; D000900:Anti-Bacterial Agents; D023303:Oxazolidinones; D014640:Vancomycin; D000069349:Linezolid; D017576:Daptomycin