Effect of edaravone on apoptosis of hippocampus neuron in seizures rats kindled by pentylenetetrazole.
OBJECTIVE
To explore the effect of edaravone (ED) on apoptosis of hippocampus neurons in seizures rats induced by pentylenetetrazole (PTZ).


METHODS
Forty-eight adult Wistar rats were randomly divided into normal control (NC) group, PTZ group, and ED group. A dose of PTZ [35 mg/(kg·day)] was intraperitoneally (i.p.) injected into the rats of PTZ group and ED group until the kindling criterion was reached. After kindling, the rats of ED group were administered i.p. with ED [0.8 mg/(kg·day)]; the rats of PTZ group were administered i.p. with normal saline. After 30 min, seizures were induced by administering PTZ i.p. The influence of ED on Fas, Caspase-3, and Survivin protein immunoreactivity on hippocampus neurons was studied by immunohistochemistry method.


RESULTS
Fas and Caspase-3 positive cells and optical density in hippocampus in PTZ group were more than that of ED group and NC group (all p < 0.01), but Survivin-positive cells and optical density in hippocampus in PTZ group were less than that of ED group (p < 0.01) and were more than that of NC group (p < 0.05); Fas- and Caspase-3-positive cells and optical density in hippocampus in ED group were more than that of NC group (all p < 0.05), but Survivin-positive cells and optical density in hippocampus in ED group were more than that of NC group (p < 0.01).


CONCLUSIONS
Seizure can induce apoptosis of hippocampus neurons on seizures rats, but ED can resist the apoptosis of hippocampus neurons by increased expression of Survivin and decreased expression of Fas and Caspase-3.
Department of Neurology, Zhongnan Hospital of Wuhan University; Department of Neurology, The First Affiliated Hospital of Yangtze University, China. jiubingcheng@126.com.
X-L
XL
Cheng
J J
JJ
Zhang
D000818:Animals; D000983:Antipyrine / Q000031:analogs & derivatives* / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D000077553:Edaravone; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism; D007696:Kindling, Neurologic / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D010433:Pentylenetetrazole / Q000009:adverse effects; D051381:Rats; D017208:Rats, Wistar; D012640:Seizures / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D000077022:Survivin; D019014:fas Receptor / Q000378:metabolism
C516989:Birc5 protein, rat; C491898:Fas protein, rat; D008869:Microtubule-Associated Proteins; D000077022:Survivin; D019014:fas Receptor; D053148:Caspase 3; D000077553:Edaravone; D000983:Antipyrine; D010433:Pentylenetetrazole