A gas chromatography-mass spectrometry method for the determination of pogostone in canine plasma and its application to a pharmacokinetic study.
In this study, a simple and selective gas chromatography-mass spectrometry method was developed and validated for the determination of pogostone in canine plasma. Liquid-liquid extraction was used to separate pogostone from canine plasma, and the mean extraction recovery rates of pogostone and the internal standard (isoalantolactone) were 80.61 and 75.89%, respectively. Plastic centrifuge tubes were inadequate for the plasma sample treatment procedure because of the adsorption effect of pogostone on the inner surface. The chromatographic separation was performed on a capillary column of Agilent HP-5ms, and the spectrometer was operated in an electron-impact ionization with an electron multiplier voltage mode. The standard curve was linear over the concentration range of 1.02-406 ng/mL (r > 0.99). The intra- and interday accuracies for pogostone at four concentrations were 97.77-99.92% and 98.51-100.22%, respectively. The relative standard deviations were <15%. The method was successfully applied to a pharmacokinetic study after the oral administration of pogostone to beagle dogs.
Pharmacy College, Chengdu University of Traditional Chinese Medicine, No.1166, Liutai Avenue, Chengdu 61137, People's Republic of China.
Ruoqi
R
Zhang
Pharmacy College, Chengdu University of Traditional Chinese Medicine, No.1166, Liutai Avenue, Chengdu 61137, People's Republic of China pengchengchengdu@126.com.
Cheng
C
Peng
Pharmacy College, Chengdu University of Traditional Chinese Medicine, No.1166, Liutai Avenue, Chengdu 61137, People's Republic of China.
Yunxia
Y
Li
Pharmacy College, Chengdu University of Traditional Chinese Medicine, No.1166, Liutai Avenue, Chengdu 61137, People's Republic of China.
Liang
L
Xiong
Pharmacy College, Chengdu University of Traditional Chinese Medicine, No.1166, Liutai Avenue, Chengdu 61137, People's Republic of China.
Xiaohong
X
Gong
D000818:Animals; D001774:Blood Chemical Analysis; D004285:Dogs; D004355:Drug Stability; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D016014:Linear Models; D059625:Liquid-Liquid Extraction; D008297:Male; D009822:Oils, Volatile / Q000032:analysis* / Q000737:chemistry / Q000493:pharmacokinetics*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012717:Sesquiterpenes
D009822:Oils, Volatile; C483658:Pogostone; D012717:Sesquiterpenes; C032787:isoalantolactone