New quinoline alkaloid from Ruta graveolens aerial parts and evaluation of the antifertility activity.
Bioassay-guided isolation of methanol extract of Ruta graveolens L. leaves yielded a new quinoline alkaloid, (4S) 1,4-dihydro-4-methoxy-1,4-dimethyl-3-(3-methylbut-2-enyl)quinoline 2,7-diol, and nine phenolic compounds including rutin as a major compound. Structures of the isolated compounds were determined by using chromatography, UV, HR-ESI-MS and 1D/2D (1)H/(13)C NMR spectroscopy. The uterotonic activity of methanol extract fractions (ethyl acetate, n-butanol and aqueous fraction) as well as the isolated major compounds was tested in the isolated mouse uterus in vitro. The n-butanol-soluble fraction was found to demonstrate the most potent uterotonic activity in a dose-dependent manner, also the major isolated compound rutin revealed the occurrence of an uterotonic response, which was maximum at a concentration level of 0.25 mg/mL, accounting for 68.7% of that exhibited by the chosen concentration of oxytocin.
a Chemistry of Tannins Department , National Research Centre , Dokki, Cairo , Egypt.
Josline Y
JY
Salib
Sayed A
SA
El-Toumy
Emad M
EM
Hassan
Nabila H
NH
Shafik
Sally M
SM
Abdel-Latif
Ignacio
I
Brouard
D020001:1-Butanol / Q000737:chemistry; D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D000818:Animals; D004305:Dose-Response Relationship, Drug; D004534:Egypt; D005260:Female; D051379:Mice; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D010121:Oxytocin / Q000032:analysis; D018515:Plant Leaves / Q000737:chemistry; D011804:Quinolines / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D017207:Rats, Sprague-Dawley; D012098:Reproduction / Q000187:drug effects*; D032105:Ruta / Q000737:chemistry*; D012431:Rutin / Q000032:analysis
C000593284:(4S) 1,4-dihydro-4-methoxy-1,4-dimethyl-3-(3-methylbut-2-enyl)quinoline 2,7-diol; D000470:Alkaloids; D011804:Quinolines; D010121:Oxytocin; D012431:Rutin; D020001:1-Butanol
Ruta graveolens L.; polyphenolic metabolites; uterotonic activity