title: Colonisation of a phage susceptible Campylobacter jejuni population in two phage positive broiler flocks.
abstract: The pathogens Campylobacter jejuni and Campylobacter coli are commensals in the poultry intestine and campylobacteriosis is one of the most frequent foodborne diseases in developed and developing countries. Phages were identified to be effective in reducing intestinal Campylobacter load and this was evaluated, in the first field trials which were recently carried out. The aim of this study was to further investigate Campylobacter population dynamics during phage application on a commercial broiler farm. This study determines the superiority in colonisation of a Campylobacter type found in a field trial that was susceptible to phages in in vitro tests. The colonisation factors, i.e. motility and gamma glutamyl transferase activity, were increased in this type. The clustering in phylogenetic comparisons of MALDI-TOF spectra did not match the ST, biochemical phenotype and phage susceptibility. Occurrence of Campylobacter jejuni strains and phage susceptibility types with different colonisation potential seem to play a very important role in the success of phage therapy in commercial broiler houses. Thus, mechanisms of both, phage susceptibility and Campylobacter colonisation should be further investigated and considered when composing phage cocktails.
authors.affiliation: Institute of Food Quality and Food Safety, University of Veterinary Medicine Hannover, Hannover, Germany.
authors.forename: Sophie
authors.initials: S
authors.lastname: Kittler
authors.affiliation: Clinic for Poultry, University of Veterinary Medicine Hannover, Hannover, Germany.
authors.forename: Samuel
authors.initials: S
authors.lastname: Fischer
authors.affiliation: Institute of Food Quality and Food Safety, University of Veterinary Medicine Hannover, Hannover, Germany.
authors.forename: Amir
authors.initials: A
authors.lastname: Abdulmawjood
authors.affiliation: Clinic for Poultry, University of Veterinary Medicine Hannover, Hannover, Germany.
authors.forename: Gerhard
authors.initials: G
authors.lastname: Glünder
authors.affiliation: Institute of Food Quality and Food Safety, University of Veterinary Medicine Hannover, Hannover, Germany.
authors.forename: Günter
authors.initials: G
authors.lastname: Klein
mesh_terms: D000465:Algorithms; D000818:Animals; D015373:Bacterial Typing Techniques; D002169:Campylobacter Infections / Q000382:microbiology / Q000662:veterinary*; D016123:Campylobacter jejuni / Q000145:classification / Q000821:virology*; D016000:Cluster Analysis; D019295:Computational Biology / Q000379:methods; D005516:Food Microbiology; D010641:Phenotype; D010802:Phylogeny; D011200:Poultry; D011201:Poultry Diseases / Q000382:microbiology*; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D005723:gamma-Glutamyltransferase / Q000378:metabolism
chemical_list: D005723:gamma-Glutamyltransferase
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