title: Purification and characterization of novel cationic peroxidases from Asparagus acutifolius L. with biotechnological applications.
abstract: Four novel basic peroxidases, named AaP-1, AaP-2, AaP-3, and AaP-4, were purified from Asparagus acutifolius L. seeds by cation-exchange and gel filtration chromatographies. The four proteins showed a similar electrophoretic mobility of 46 kDa while, by MALDI-TOF MS, different Mr values of 42758.3, 41586.9, 42796.3, and 41595.5 were determined for AaP-1, AaP-2, AaP-3, and AaP-4, respectively. N-terminal sequences of AaPs 1-4 up to residue 20 showed a high percentage of identity with the peroxidase from Glycine max. In addition, AaP-1, AaP-2, AaP-3, and AaP-4 were found to be glycoproteins, containing 21.75, 22.27, 25.62, and 18.31 % of carbohydrates, respectively. Peptide mapping and MALDI-TOF MS analysis of AaPs 1-4 showed that the structural differences between AaP-1 and AaP-2 and AaP-3 and AaPs-4 were mainly due to their glycan content. We also demonstrate that AaPs were able to remove phenolic compounds from olive oil mill wastewaters with a higher catalytic efficiency with respect to horseradish peroxidase, thus representing candidate enzymes for potential biotechnological applications in the environmental field.
authors.affiliation: Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, Second University of Naples, Via Vivaldi 43, 81100, Caserta, Italy.
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Guida
authors.forename: Maria
authors.initials: M
authors.lastname: Cantarella
authors.forename: Angela
authors.initials: A
authors.lastname: Chambery
authors.forename: Maria C
authors.initials: MC
authors.lastname: Mezzacapo
authors.forename: Augusto
authors.initials: A
authors.lastname: Parente
authors.forename: Nicola
authors.initials: N
authors.lastname: Landi
authors.forename: Valeria
authors.initials: V
authors.lastname: Severino
authors.forename: Antimo
authors.initials: A
authors.lastname: Di Maro
mesh_terms: D000595:Amino Acid Sequence; D027761:Asparagus Plant / Q000201:enzymology* / Q000235:genetics; D001709:Biotechnology; D006031:Glycosylation; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D008970:Molecular Weight; D000069463:Olive Oil; D010449:Peptide Mapping; D010544:Peroxidases / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D010938:Plant Oils; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D012639:Seeds / Q000201:enzymology; D017386:Sequence Homology, Amino Acid; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D062065:Wastewater; D018508:Water Purification
chemical_list: D000069463:Olive Oil; D010544:Peroxidases; D010938:Plant Oils; D010940:Plant Proteins; D062065:Wastewater
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