title: Non-healing old world cutaneous leishmaniasis caused by L. infantum in a patient from Spain.
abstract: BACKGROUND
  The prevalence of Old World Cutaneous Leishmaniasis in the Mediterranean region is increasing and in Southern Europe often caused by Leishmania infantum. Spontaneous healing of cutaneous leishmaniasis is commonly observed, especially if caused by L. major, whereas L. infantum associated lesions have been reported with longer disease duration and decreased tendency for self-limitation, however, available information is sparse.
  
  
  METHODS
  We report the case of an otherwise healthy woman from Southern Spain who presented with a seven years persistent, non-healing, painless, central ulcerated, nodular cutaneous lesion with a diameter of 2 cm of the forearm. Cutaneous leishmaniasis was diagnosed by smear and histology, showing large amounts of leishmania amastigotes in subepidermal histiocytes and extensive lymphocyte and plasma cell inflammation. L. infantum as the causative pathogen was confirmed by restriction fragment length polymorphism and microsatellite-PCR. Systemic or visceral involvement was excluded by negative leishmania serology and clinical presentation, relevant concomitant diseases or immunosuppression were excluded including quantification of immunoglobulin levels and lymphocyte phenotyping. Topical and systemic anti-infectious treatment options, often limited in terms of efficacy, tolerability and long lasting treatment duration, were considered. Treatment was successfully performed by surgical extraction in local anaesthesia only.
  
  
  CONCLUSIONS
  To our knowledge this is the longest reported duration of a L. infantum associated cutaneous leishmaniasis indicating a potential long lasting natural evolution of the disease in an otherwise healthy and immunocompetent patient, however, high parasite density may have reflected a lack of a L. infantum specific immune response. Complete surgical extraction can be successfully performed as treatment.
authors.affiliation: Division of Infectious Diseases and Tropical Medicine, Medical Center of the University of Munich, Leopoldstr, 5, 80802 Munich, Germany. akroidl@lrz.uni-muenchen.de.
authors.forename: Arne
authors.initials: A
authors.lastname: Kroidl
authors.forename: Inge
authors.initials: I
authors.lastname: Kroidl
authors.forename: Gisela
authors.initials: G
authors.lastname: Bretzel
authors.forename: Thomas
authors.initials: T
authors.lastname: Löscher
mesh_terms: D000328:Adult; D005260:Female; D006801:Humans; D018314:Leishmania infantum / Q000235:genetics / Q000302:isolation & purification*; D016773:Leishmaniasis, Cutaneous / Q000175:diagnosis* / Q000469:parasitology / Q000601:surgery; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D013030:Spain
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