title: Mechanosensitive physiology of Chlamydomonas reinhardtii under direct membrane distortion.
abstract: Cellular membrane distortion invokes variations in cellular physiology. However, lack of an appropriate system to control the stress and facilitate molecular analyses has hampered progress of relevant studies. In this study, a microfluidic system that finely manipulates membrane distortion of Chlamydomonas reinhardtii (C. reinhardtii) was developed. The device facilitated a first-time demonstration that directs membrane distortion invokes variations in deflagellation, cell cycle, and lipid metabolism. C. reinhardtii showed a prolonged G1 phase with an extended total cell cycle time, and upregulated Mat3 regulated a cell size and cell cycle. Additionally, increased TAG compensated for the loss of cell mass. Overall, this study suggest that cell biology that requires direct membrane distortion can be realized using this system, and the implication of cell cycle with Mat3 expression of C. reinhardtii was first demonstrated. Finally, membrane distortion can be an attractive inducer for biodiesel production since it is reliable and robust.
authors.affiliation: Department of Biological Engineering, Inha University, Incheon, 402-751, Korea.
authors.forename: Seul Ki
authors.initials: SK
authors.lastname: Min
authors.affiliation: Department of Biological Engineering, Inha University, Incheon, 402-751, Korea.
authors.forename: Gwang Heum
authors.initials: GH
authors.lastname: Yoon
authors.affiliation: Department of Biological Engineering, Inha University, Incheon, 402-751, Korea.
authors.forename: Jung Hyun
authors.initials: JH
authors.lastname: Joo
authors.affiliation: Department of Chemical and Biological Engineering, Korea University, Seoul, 136-713, Korea.
authors.forename: Sang Jun
authors.initials: SJ
authors.lastname: Sim
authors.affiliation: Department of Biological Engineering, Inha University, Incheon, 402-751, Korea.
authors.forename: Hwa Sung
authors.initials: HS
authors.lastname: Shin
mesh_terms: D017209:Apoptosis; D002118:Calcium / Q000378:metabolism; D049109:Cell Proliferation; D048429:Cell Size; D016825:Chlamydomonas reinhardtii / Q000378:metabolism*; D016193:G1 Phase; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation; D059828:Plant Cells / Q000502:physiology; D015800:Protozoan Proteins / Q000378:metabolism; D016160:Retinoblastoma Protein / Q000378:metabolism; D013314:Stress, Mechanical; D015854:Up-Regulation
chemical_list: D015800:Protozoan Proteins; D016160:Retinoblastoma Protein; C432865:mat3 protein, Chlamydomonas; D002118:Calcium
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