title: The +1245g/t polymorphisms in the collagen type I alpha 1 (col1a1) gene in polish skiers with anterior cruciate ligament injury.
abstract: OBJECTIVE
  The aim of this study was to examine the association of +1245G/T polymorphisms in the COL1A1 gene with ACL ruptures in Polish male recreational skiers in a case-control study.
  
  
  METHODS
  A total of 138 male recreational skiers with surgically diagnosed primary ACL ruptures, all of whom qualified for ligament reconstruction, were recruited for this study. The control group comprised 183 apparently healthy male skiers with a comparable level of exposure to ACL injury, none of whom had any self-reported history of ligament or tendon injury. DNA samples extracted from the oral epithelial cells were genotyped for the +1245G/T polymorphisms using real-time PCR method.
  
  
  RESULTS
  Genotype distributions among cases and controls conformed to Hardy-Weinberg equilibrium (p = 0.2469 and p = 0.33, respectively). There was a significant difference in the genotype distribution between skiers and controls (p = 0.045, Fisher's exact test). There was no statistical difference in allele distribution: OR 1.43 (0.91-2.25), p = 0.101 (two-sided Fisher's exact test).
  
  
  CONCLUSIONS
  The risk of ACL ruptures was around 1.43 times lower in carriers of a minor allele G as compared to carriers of the allele T.
authors.affiliation: University of Szczecin, Department of Physical Culture and Health Promotion, Szczecin, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Stępien-Słodkowska
authors.affiliation: University of Szczecin, Department of Physical Culture and Health Promotion, Szczecin, Poland ; Galen Medical Center, Bieruń, Poland.
authors.forename: K
authors.initials: K
authors.lastname: Ficek
authors.affiliation: University of Szczecin, Department of Physical Culture and Health Promotion, Szczecin, Poland.
authors.forename: J
authors.initials: J
authors.lastname: Eider
authors.affiliation: University of Szczecin, Department of Physical Culture and Health Promotion, Szczecin, Poland ; Academy of Physical Education and Sport, Department of Sport Education, Gdańsk, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Leońska-Duniec
authors.affiliation: University of Szczecin, Department of Physical Culture and Health Promotion, Szczecin, Poland ; University of Szczecin, Department of Genetics, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Maciejewska-Karłowska
authors.affiliation: University of Szczecin, Department of Physical Culture and Health Promotion, Szczecin, Poland ; University of Szczecin, Department of Genetics, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Sawczuk
authors.affiliation: Academy of Physical Education and Sport, Department of Sport Education, Gdańsk, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Zarębska
authors.affiliation: Academy of Physical Education and Sport, Department of Sport Education, Gdańsk, Poland.
authors.forename: Z
authors.initials: Z
authors.lastname: Jastrzębski
authors.affiliation: West Pomeranian Technological University, Department of Physical Education and Sport, Szczecin, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Grenda
authors.affiliation: University of Szczecin, Department of Physical Culture and Health Promotion, Szczecin, Poland.
authors.forename: K
authors.initials: K
authors.lastname: Kotarska
authors.affiliation: Academy of Physical Education and Sport, Department of Sport Education, Gdańsk, Poland.
authors.forename: P
authors.initials: P
authors.lastname: Cięszczyk
keywords: COL1A1 gene; anterior cruciate ligament (ACL) rupture; collagen; polymorphism; skiers
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