title: [Influence and analysis of seven kinds of hemoglobin variants on HbA1c detection].
abstract: OBJECTIVE
  To explore the effects of seven kinds of hemoglobin variants on two HbA1c detection methods.
  
  
  METHODS
  Twenty-five hemoglobin variant samples (Hb D, S, Q, G, J, E & F) and 40 control samples were from February 2012 to April 2013 collected. All samples were tested by ion exchange-high performance liquid chromatography system (IE-HPLC) and affinity chromatograghy high performance liquid chromatography (AC-HPLC) respectively.We compared the coincidence between HbA1c results of two instruments and blood glucose and observed the difference between variant and control groups for two methods using statistic software SPSS 19.0.
  
  
  RESULTS
  A high consistency existed between IE-HPLC and AC-HPLC in the control group with no hemoglobin variants (6.68% ± 1.87% vs 6.64% ± 1.99%, P > 0.05) . For the hemoglobin variants group, the results of HbA1c via IE-HPLC were interfered by hemoglobin variants (3.57% ± 3.51% than 4.95% ± 0.57%, P < 0.05). However, HbA1c detection of AC-HPLC had no interference with hemoglobin variants and it demonstrated an excellent correlation with blood glucose.
  
  
  CONCLUSIONS
  The results of HbA1c in blood samples containing common hemoglobin variants may be interfered on IE-HPLC to be falsely lower or higher.Only detecting glycated hemoglobin with strong specificity,AC-HPLC is well-correlated with blood glucose and its results are not interfered by common variant hemoglobin.
authors.affiliation: Department of Clinical Laboratory Medicine, Peking Union Medical College Hospital & Chinese Academy of Medical Sciences, Beijing 100730, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Clinical Laboratory Medicine, Peking Union Medical College Hospital & Chinese Academy of Medical Sciences, Beijing 100730, China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Department of Clinical Laboratory Medicine, Peking Union Medical College Hospital & Chinese Academy of Medical Sciences, Beijing 100730, China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Hao
authors.affiliation: Department of Clinical Laboratory Medicine, Peking Union Medical College Hospital & Chinese Academy of Medical Sciences, Beijing 100730, China.
authors.forename: Shuling
authors.initials: S
authors.lastname: Chi
authors.affiliation: Department of Clinical Laboratory Medicine, Peking Union Medical College Hospital & Chinese Academy of Medical Sciences, Beijing 100730, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Zhao
authors.affiliation: Department of Clinical Laboratory Medicine, Peking Union Medical College Hospital & Chinese Academy of Medical Sciences, Beijing 100730, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Clinical Laboratory Medicine, Peking Union Medical College Hospital & Chinese Academy of Medical Sciences, Beijing 100730, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Clinical Laboratory Medicine, Peking Union Medical College Hospital & Chinese Academy of Medical Sciences, Beijing 100730, China. Email: douyling@163.com.
authors.forename: Yaling
authors.initials: Y
authors.lastname: Dou
mesh_terms: D000328:Adult; D001786:Blood Glucose / Q000032:analysis; D016022:Case-Control Studies; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003920:Diabetes Mellitus / Q000097:blood*; D014644:Genetic Variation; D006442:Glycated Hemoglobin / Q000032:analysis*; D006454:Hemoglobins / Q000145:classification; D006801:Humans; D008875:Middle Aged; D055815:Young Adult
chemical_list: D001786:Blood Glucose; D006442:Glycated Hemoglobin; D006454:Hemoglobins