title: Cellular extract facilitates nuclear reprogramming by altering DNA methylation and pluripotency gene expression.
abstract: The functional reprogramming of a differentiated cell to a pluripotent state presents potential beneficial applications in disease mechanisms and regenerative medicine. Epigenetic modifications enable differentiated cells to perpetuate molecular memory to retain their identity. Therefore, the aim of this study was to investigate the reprogramming modification of yak fibroblast cells that were permeabilized and incubated in the extracts of mesenchymal stem cells derived from mice adipose tissue [adipose-derived stem cells (ADSCs)]. According to the results, the treatment of ADSC extracts promoted colony formation. Moreover, pluripotent gene expression was associated with the loss of repressive histone modifications and increased global demethylation. The genes Col1a1 and Col1a2, which are typically found in differentiated cells only, demonstrated decreased expression and increased methylation in the 5'-flanking regulatory regions. Moreover, yak fibroblast cells that were exposed to ADSC extracts resulted in significantly different eight-cell and blastocyst formation rates of cloned embryos compared with their untreated counterparts. This investigation provides the first evidence that nuclear reprogramming of yak fibroblast cells is modified after the ADSC extract treatment. This research also presents a methodology for studying the dedifferentiation of somatic cells that can potentially lead to an efficient way of reprogramming somatic cells toward a pluripotent state without genetic alteration.
authors.affiliation: 1 College of Life Science and Technology, Southwest University for Nationalities , Chengdu, Sichuan, 610041, China .
authors.forename: Xian-Rong
authors.initials: XR
authors.lastname: Xiong
authors.forename: Dao-Liang
authors.initials: DL
authors.lastname: Lan
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.forename: Xiang-Dong
authors.initials: XD
authors.lastname: Zi
authors.forename: Li
authors.initials: L
authors.lastname: Ma
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
mesh_terms: D024506:5' Flanking Region; D000107:Acetylation; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002478:Cells, Cultured; D065150:Cellular Reprogramming; D003094:Collagen / Q000235:genetics; D019175:DNA Methylation; D017931:DNA Primers; D005260:Female; D015870:Gene Expression; D006657:Histones / Q000378:metabolism; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D016133:Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D006657:Histones; D003094:Collagen
references.citation: Cloning Stem Cells. 2008 Dec;10(4):535-42
references.pmid: 19049416
references.citation: Nature. 2010 Feb 25;463(7284):1042-7
references.pmid: 20027182
references.citation: Science. 2001 Aug 10;293(5532):1093-8
references.pmid: 11498580
references.citation: Nature. 2009 Sep 3;461(7260):91-4
references.pmid: 19672243
references.citation: Stem Cells. 2007 Apr;25(4):818-27
references.pmid: 17420225
references.citation: Theriogenology. 2010 Nov;74(8):1439-49
references.pmid: 20708232
references.citation: Cell. 2007 Nov 30;131(5):861-72
references.pmid: 18035408
references.citation: Theriogenology. 2013 Feb;79(3):462-9
references.pmid: 23186945
references.citation: Reprod Fertil Dev. 2014 Aug;26(7):1017-31
references.pmid: 25145414
references.citation: Biores Open Access. 2012 Apr;1(2):79-87
references.pmid: 23515109
references.citation: Science. 2007 Dec 21;318(5858):1917-20
references.pmid: 18029452
references.citation: Theriogenology. 2006 Jan 7;65(1):178-91
references.pmid: 16266745
references.citation: Cell Reprogram. 2010 Oct;12(5):609-16
references.pmid: 20936909
references.citation: Science. 2005 Aug 26;309(5739):1369-73
references.pmid: 16123299
references.citation: Trends Genet. 2011 Dec;27(12):516-25
references.pmid: 21940062
references.citation: Cell. 2008 Feb 22;132(4):567-82
references.pmid: 18295576
references.citation: Mol Biol Rep. 2010 Mar;37(3):1197-202
references.pmid: 19263240
references.citation: Cell. 2006 Aug 25;126(4):663-76
references.pmid: 16904174
references.citation: Cell Reprogram. 2011 Aug;13(4):315-29
references.pmid: 21718106
references.citation: Nat Genet. 2007 Mar;39(3):295-302
references.pmid: 17325680
references.citation: Cell Res. 2011 Mar;21(3):381-95
references.pmid: 21321607
references.citation: Theriogenology. 2012 Dec;78(9):2110-9
references.pmid: 23110954
references.citation: Nature. 1997 Feb 27;385(6619):810-3
references.pmid: 9039911
references.citation: Biochem Biophys Res Commun. 2004 Apr 9;316(3):834-41
references.pmid: 15033476
references.citation: Mol Biol Cell. 2007 May;18(5):1543-53
references.pmid: 17314394
references.citation: Mol Biol Cell. 2005 Dec;16(12):5719-35
references.pmid: 16195347
references.citation: Science. 2008 Dec 19;322(5909):1811-5
references.pmid: 19095934
references.citation: Nature. 2008 Jul 3;454(7200):49-55
references.pmid: 18509334
references.citation: Cell Reprogram. 2012 Jun;14(3):235-47
references.pmid: 22468998
references.citation: Exp Cell Res. 2008 Aug 15;314(14):2634-42
references.pmid: 18571647
references.citation: Bioinformatics. 2005 Nov 1;21(21):4067-8
references.pmid: 16141249
references.citation: Nucleic Acids Res. 2009 Apr;37(6):e45
references.pmid: 19237396
references.citation: Cell Reprogram. 2013 Aug;15(4):293-300
references.pmid: 23790013