title: Xenoimplantation of an extracellular-matrix-derived, biphasic, cell-scaffold construct for repairing a large femoral-head high-load-bearing osteochondral defect in a canine model.
abstract: This study was aimed to develop an ECM-derived biphasic scaffold and to investigate its regeneration potential loaded with BM-MSCs in repair of large, high-load-bearing osteochondral defects of the canine femoral head. The scaffolds were fabricated using cartilage and bone ECM as a cartilage and bone layer, respectively. Osteochondral constructs were fabricated using induced BM-MSCs and the scaffold. Osteochondral defects (11 mm diameter × 10 mm depth) were created on femoral heads of canine and treated with the constructs. The repaired tissue was evaluated for gross morphology, radiography, histological, biomechanics at 3 and 6 months after implantation. Radiography revealed that femoral heads slightly collapsed at 3 months and severely collapsed at 6 months. Histology revealed that some defects in femoral heads were repaired, but with fibrous tissue or fibrocartilage, and femoral heads with different degrees of collapse. The bone volume fraction was lower for subchondral bone than normal femoral bone at 3 and 6 months. Rigidity was lower in repaired subchondral bone than normal femoral bone at 6 months. The ECM-derived, biphasic scaffold combined with induced BM-MSCs did not successfully repair large, high-load-bearing osteochondral defects of the canine femoral head. However, the experience can help improve the technique of scaffold fabrication and vascularization.
authors.affiliation: Institute of Orthopedics, Chinese PLA General Hospital, Beijing 100853, China ; Department of Spine Surgery, Tianjin Hospital, Tianjin 300211, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Qiang
authors.affiliation: Stomatological Hospital of Tianjin Medical University, Tianjin 300070, China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Yanhong
authors.affiliation: Institute of Orthopedics, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Jiang
authors.affiliation: Institute of Orthopedics, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Shibi
authors.affiliation: Institute of Orthopedics, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Guo
authors.initials: G
authors.lastname: Quanyi
authors.affiliation: Department of Spine Surgery, Tianjin Hospital, Tianjin 300211, China.
authors.forename: Ma
authors.initials: M
authors.lastname: Xinlong
authors.affiliation: Department of Spine Surgery, Tianjin Hospital, Tianjin 300211, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Qun
authors.affiliation: Department of Spine Surgery, Tianjin Hospital, Tianjin 300211, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Baoshan
authors.affiliation: Institute of Orthopedics, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Bin
authors.affiliation: Institute of Orthopedics, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Wang
authors.initials: W
authors.lastname: Aiyuan
authors.affiliation: Institute of Orthopedics, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Li
authors.affiliation: Institute of Orthopedics, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Wengjing
authors.affiliation: Department of Spine Surgery, Tianjin Hospital, Tianjin 300211, China.
authors.forename: Zeng
authors.initials: Z
authors.lastname: Chao
mesh_terms: D000818:Animals; D004285:Dogs; D019544:Equipment Failure Analysis; D005109:Extracellular Matrix / Q000737:chemistry* / Q000637:transplantation*; D005271:Femur Head Necrosis / Q000175:diagnosis / Q000503:physiopathology* / Q000601:surgery*; D017102:Fracture Healing / Q000502:physiology; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000295:instrumentation* / Q000379:methods; D010012:Osteogenesis / Q000502:physiology; D011474:Prosthesis Design; D054457:Tissue Scaffolds; D016896:Treatment Outcome; D016474:Weight-Bearing
references.citation: Biomaterials. 2007 May;28(15):2479-90
references.pmid: 17258316
references.citation: Tissue Eng. 2000 Aug;6(4):441-8
references.pmid: 10992439
references.citation: Clin Orthop Relat Res. 2002 Feb;(395):81-98
references.pmid: 11937868
references.citation: Clin Orthop Relat Res. 2010 Oct;468(10):2715-24
references.pmid: 20224959
references.citation: Clin Orthop Relat Res. 2001 May;(386):71-8
references.pmid: 11347851
references.citation: J Bone Joint Surg Am. 2006 Nov;88 Suppl 3:167-72
references.pmid: 17079384
references.citation: Clin Orthop Relat Res. 2007 Jun;459:216-21
references.pmid: 17308484
references.citation: Am J Sports Med. 1989 May-Jun;17(3):393-400
references.pmid: 2729490
references.citation: Chin Med J (Engl). 2011 Dec;124(23):3930-8
references.pmid: 22340321
references.citation: Bone. 2002 May;30(5):759-64
references.pmid: 11996916
references.citation: Biomaterials. 2008 May;29(15):2378-87
references.pmid: 18313139
references.citation: Biomaterials. 2008 Sep;29(26):3539-46
references.pmid: 18538387
references.citation: J Biomed Mater Res B Appl Biomater. 2011 Feb;96(2):316-25
references.pmid: 21210512
references.citation: J Orthop Surg Res. 2011 Oct 17;6:55
references.pmid: 22004681
references.citation: Leukemia. 2002 Jun;16(6):1160-6
references.pmid: 12040448
references.citation: J Arthroplasty. 2001 Dec;16(8 Suppl 1):134-9
references.pmid: 11742465
references.citation: Int J Oncol. 2003 Sep;23(3):681-91
references.pmid: 12888904
references.citation: Bone. 2009 Apr;44(4):590-5
references.pmid: 19162254
references.citation: Biomaterials. 2001 Jun;22(11):1279-88
references.pmid: 11336300
references.citation: J Craniofac Surg. 2004 Jan;15(1):34-7
references.pmid: 14704559
references.citation: Science. 1999 Apr 2;284(5411):143-7
references.pmid: 10102814
references.citation: J Arthroplasty. 2003 Jan;18(1):83-8
references.pmid: 12555188
references.citation: J Bone Joint Surg Am. 2006 May;88(5):1117-32
references.pmid: 16651589
references.citation: Int Orthop. 2001;24(6):316-8
references.pmid: 11294420
references.citation: Clin Orthop Relat Res. 2001 May;(386):100-5
references.pmid: 11347821
references.citation: Int Orthop. 2009 Apr;33(2):573-80
references.pmid: 18956184
references.citation: Indian J Orthop. 2009 Jan;43(1):6-16
references.pmid: 19753173
references.citation: Clin Orthop Relat Res. 2003 Dec;(417):84-92
references.pmid: 14646705