title: Spatially variant red blood cell crenation in alternating current non-uniform fields.
abstract: Alternating-current (AC) electrokinetics involve the movement and behaviors of particles or cells. Many applications, including dielectrophoretic manipulations, are dependent upon charge interactions between the cell or particle and the surrounding medium. Medium concentrations are traditionally treated as spatially uniform in both theoretical models and experiments. Human red blood cells (RBCs) are observed to crenate, or shrink due to changing osmotic pressure, over 10 min experiments in non-uniform AC electric fields. Cell crenation magnitude is examined as functions of frequency from 250 kHz to 1 MHz and potential from 10 Vpp to 17.5 Vpp over a 100 μm perpendicular electrode gap. Experimental results show higher peak to peak potential and lower frequency lead to greater cell volume crenation up to a maximum volume loss of 20%. A series of experiments are conducted to elucidate the physical mechanisms behind the red blood cell crenation. Non-uniform and uniform electrode systems as well as high and low ion concentration experiments are compared and illustrate that AC electroporation, system temperature, rapid temperature changes, medium pH, electrode reactions, and convection do not account for the crenation behaviors observed. AC electroosmotic was found to be negligible at these conditions and AC electrothermal fluid flows were found to reduce RBC crenation behaviors. These cell deformations were attributed to medium hypertonicity induced by ion concentration gradients in the spatially nonuniform AC electric fields.
authors.affiliation: Department of Chemical Engineering, Michigan Technological University, Houghton, Michigan 49931, USA.
authors.forename: Ran
authors.initials: R
authors.lastname: An
authors.affiliation: Department of Chemistry, Mississippi State University, Mississippi State, Mississippi 39762, USA.
authors.forename: David O
authors.initials: DO
authors.lastname: Wipf
authors.affiliation: Department of Chemical Engineering, Michigan Technological University, Houghton, Michigan 49931, USA.
authors.forename: Adrienne R
authors.initials: AR
authors.lastname: Minerick
references.citation: Biophys J. 1999 Sep;77(3):1213-24
references.pmid: 10465736
references.citation: Langmuir. 2009 May 5;25(9):4988-97
references.pmid: 19320476
references.citation: Biomicrofluidics. 2009 Apr 23;3(2):22405
references.pmid: 19693340
references.citation: Electrophoresis. 2003 Nov;24(21):3703-17
references.pmid: 14613196
references.citation: Langmuir. 2008 Sep 2;24(17):9361-9
references.pmid: 18672919
references.citation: Clin Sci (Lond). 1992 Jan;82(1):9-11
references.pmid: 1310923
references.citation: IEEE Trans Ind Appl. 1997;33(3):670-678
references.pmid: 20011619
references.citation: Anal Chem. 2004 Mar 15;76(6):1571-9
references.pmid: 15018553
references.citation: Electrophoresis. 2011 Sep;32(18):2512-22
references.pmid: 21874652
references.citation: Electrophoresis. 2011 Sep;32(18):2530-40
references.pmid: 21922495
references.citation: Biochim Biophys Acta. 1998 Jul 17;1372(2):198-204
references.pmid: 9675280
references.citation: Blood. 1995 Aug 15;86(4):1595-9
references.pmid: 7632969
references.citation: Biophys J. 1999 Sep;77(3):1225-33
references.pmid: 10465737
references.citation: Biochim Biophys Acta. 1996 Oct 23;1284(2):143-52
references.pmid: 8914578
references.citation: Integr Biol (Camb). 2010 Mar;2(2-3):113-20
references.pmid: 20473389
references.citation: J Crit Care. 2010 Sep;25(3):539.e1-12
references.pmid: 20381299
references.citation: Biophys J. 1990 Jul;58(1):1-12
references.pmid: 2383626
references.citation: IET Nanobiotechnol. 2007 Jun;1(3):36-43
references.pmid: 17506595
references.citation: J Biol Chem. 1975 Jan 25;250(2):786-9
references.pmid: 1112789
references.citation: Bioelectrochemistry. 2000 Jun;51(2):133-43
references.pmid: 10910161
references.citation: Nature. 1977 Aug 4;268(5619):438-41
references.pmid: 895849
references.citation: Lab Chip. 2012 Jul 7;12(13):2362-8
references.pmid: 22513827
references.citation: Anal Chem. 1998 May 1;70(9):1909-15
references.pmid: 9599586
references.citation: Electrophoresis. 2011 Sep;32(18):2436-47
references.pmid: 21874654
references.citation: Biomicrofluidics. 2013 Dec 10;7(6):64114
references.pmid: 24396548
references.citation: Biophys J. 2004 May;86(5):3304-13
references.pmid: 15111443
references.citation: Transfus Med Rev. 2002 Jan;16(1):46-60
references.pmid: 11788929