title: Characterization of Chlorococcum pamirum as a potential biodiesel feedstock.
abstract: To evaluate the potential of Chlorococcum pamirum for producing biodiesel, the effects of nitrogen, phosphate, initial cell concentrations and NaCl on lipid accumulation and growth were studied. The highest specific growth rate (μ(max)), biomass productivity and lipid content achieved was 1.888 d(-1), 350.1 mg L(-1) day(-1), and 64.9%, respectively. Under nitrogen-deficient condition, the cells accumulated lipids faster at low initial cell concentration. Additional NaCl to nitrogen-deficient media accelerated the lipid accumulation. When adding 10 g L(-1) NaCl to nitrogen-deficient media, the lipid content and productivity of cells cultured outdoors with high initial cell concentration increased from 38.2% and 153 mg L(-1) day(-1) to 54.3% and 192 mg L(-1) day(-1) respectively. Moreover, NaCl enhanced the saturated fatty acids content from 56.40% to 73.41% of total fatty acids. The results show that C. pamirum is a promising organism for biofuel production.
authors.affiliation: Microalgae Laboratory, School of Resource & Environmental Engineering, Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Hubei University of Technology, Wuhan 430068, China; Key Laboratory of Renewable Energy and Gas Hydrate, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, China; Algaen Corporation, 925 West Northwest Blvd., Winston-Salem, NC 27101, USA.
authors.forename: Pingzhong
authors.initials: P
authors.lastname: Feng
authors.affiliation: Microalgae Laboratory, School of Resource & Environmental Engineering, Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Hubei University of Technology, Wuhan 430068, China.
authors.forename: Zhongyang
authors.initials: Z
authors.lastname: Deng
authors.affiliation: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei 430072, China.
authors.forename: Zhengyu
authors.initials: Z
authors.lastname: Hu
authors.affiliation: Key Laboratory of Renewable Energy and Gas Hydrate, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, China.
authors.forename: Zhongming
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Microalgae Laboratory, School of Resource & Environmental Engineering, Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Hubei University of Technology, Wuhan 430068, China; Algaen Corporation, 925 West Northwest Blvd., Winston-Salem, NC 27101, USA. Electronic address: lf1230nc@yahoo.com.
authors.forename: Lu
authors.initials: L
authors.lastname: Fan
mesh_terms: D056804:Biofuels; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology; D000460:Chlorophyta / Q000166:cytology / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D008055:Lipids / Q000096:biosynthesis; D009566:Nitrates / Q000494:pharmacology; D009584:Nitrogen / Q000494:pharmacology; D010710:Phosphates / Q000494:pharmacology; D010758:Phosphorus / Q000494:pharmacology; D017680:Potassium Compounds / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology
chemical_list: D056804:Biofuels; D008055:Lipids; D009566:Nitrates; D010710:Phosphates; D017680:Potassium Compounds; D010758:Phosphorus; D012965:Sodium Chloride; C031618:sodium nitrate; C013216:potassium phosphate; D009584:Nitrogen
keywords: Biodiesel; Chlorococcum pamirum; Lipid accumulation; Microalgae cultivation; Salt stress