title: DNA damage and oxidative stress induced by acetylsalicylic acid in Daphnia magna.
abstract: Acetylsalicylic acid is a nonsteroidal anti-inflammatory widely used due to its low cost and high effectiveness. This compound has been found in water bodies worldwide and is toxic to aquatic organisms; nevertheless its capacity to induce oxidative stress in bioindicators like Daphnia magna remains unknown. This study aimed to evaluate toxicity in D. magna induced by acetylsalicylic acid in water, using oxidative stress and DNA damage biomarkers. An acute toxicity test was conducted in order to determine the median lethal concentration (48-h LC50) and the concentrations to be used in the subsequent subacute toxicity test in which the following biomarkers were evaluated: lipid peroxidation, oxidized protein content, activity of the antioxidant enzymes superoxide dismutase, catalase, and glutathione peroxidase, and level of DNA damage. Lipid peroxidation level and oxidized protein content were significantly increased (p<0.05), and antioxidant enzymes significantly altered with respect to controls; while the DNA damage were significantly increased (p<0.05) too. In conclusion, acetylsalicylic acid induces oxidative stress and DNA damage in D. magna.
authors.affiliation: Laboratorio de Toxicología Ambiental, Facultad de Química, Universidad Autónoma del Estado de México, Paseo Colón intersección Paseo Tollocan s/n. Col. Residencial Colón, 50120 Toluca, Estado de México, Mexico. Electronic address: lmgomezo@uaemex.mx.
authors.forename: Leobardo Manuel
authors.initials: LM
authors.lastname: Gómez-Oliván
authors.affiliation: Laboratorio de Toxicología Acuática, Departamento de Farmacia, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Plan de Ayala y Carpio s/n, 11340 México D.F., Mexico.
authors.forename: Marcela
authors.initials: M
authors.lastname: Galar-Martínez
authors.affiliation: Laboratorio de Toxicología Ambiental, Facultad de Química, Universidad Autónoma del Estado de México, Paseo Colón intersección Paseo Tollocan s/n. Col. Residencial Colón, 50120 Toluca, Estado de México, Mexico.
authors.forename: Hariz
authors.initials: H
authors.lastname: Islas-Flores
authors.affiliation: Laboratorio de Toxicología Acuática, Departamento de Farmacia, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Plan de Ayala y Carpio s/n, 11340 México D.F., Mexico.
authors.forename: Sandra
authors.initials: S
authors.lastname: García-Medina
authors.affiliation: Laboratorio de Toxicología Ambiental, Facultad de Química, Universidad Autónoma del Estado de México, Paseo Colón intersección Paseo Tollocan s/n. Col. Residencial Colón, 50120 Toluca, Estado de México, Mexico.
authors.forename: Nely
authors.initials: N
authors.lastname: SanJuan-Reyes
mesh_terms: D000818:Animals; D001241:Aspirin / Q000633:toxicity*; D002374:Catalase / Q000378:metabolism; D004249:DNA Damage / Q000187:drug effects*; D003621:Daphnia / Q000378:metabolism*; D005979:Glutathione Peroxidase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D013482:Superoxide Dismutase / Q000378:metabolism; D023382:Toxicity Tests, Acute; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D014874:Water Pollutants, Chemical; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D001241:Aspirin
keywords: Acetylsalicylic acid; DNA damage; Daphnia magna; Oxidative stress