title: Controlled charged amino acids of Ti-binding peptide for surfactant-free selective adsorption.
abstract: The adsorption mechanism of titanium-binding peptide (TBP) on metal oxide substrates was investigated by evaluating the adsorption behavior of ferritins with various alanine-substituted TBPs. Results revealed that (a) a positively charged amino acid, lysine (K) or arginine (R), in TBP can anchor ferritin to negative zeta-potential substrates, (b) the adsorption force of K is stronger than R, and (c) local electrostatic interactions and flexibility of TBP directly affect adsorption. Based on these findings, selective ferritin adsorption on SiO2 with TiOX patterned surfaces in a surfactant-free condition was demonstrated. Alanine-substituted TBP with one positively charged amino acid (K) and one negatively charged amino acid (D), achieved ferritin-selective adsorption without a surfactant. The importance of controlled electrostatic forces between TBP and a substrate for selective adsorption without a surfactant was clearly demonstrated.
authors.affiliation: Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan; Japan Science and Technology Agency, CREST, 5 Sanbancho, Chiyoda-ku, Tokyo 102-0075, Japan. Electronic address: fukuta@cc.tuat.ac.jp.
authors.forename: Megumi
authors.initials: M
authors.lastname: Fukuta
authors.affiliation: Graduate School of Materials Science, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan; Japan Science and Technology Agency, CREST, 5 Sanbancho, Chiyoda-ku, Tokyo 102-0075, Japan.
authors.forename: Bin
authors.initials: B
authors.lastname: Zheng
authors.affiliation: Graduate School of Materials Science, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan; Japan Science and Technology Agency, CREST, 5 Sanbancho, Chiyoda-ku, Tokyo 102-0075, Japan.
authors.forename: Mutsunori
authors.initials: M
authors.lastname: Uenuma
authors.affiliation: Graduate School of Materials Science, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan.
authors.forename: Naofumi
authors.initials: N
authors.lastname: Okamoto
authors.affiliation: Graduate School of Materials Science, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan; Japan Science and Technology Agency, CREST, 5 Sanbancho, Chiyoda-ku, Tokyo 102-0075, Japan.
authors.forename: Yukiharu
authors.initials: Y
authors.lastname: Uraoka
authors.affiliation: Graduate School of Materials Science, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan; Japan Science and Technology Agency, CREST, 5 Sanbancho, Chiyoda-ku, Tokyo 102-0075, Japan.
authors.forename: Ichiro
authors.initials: I
authors.lastname: Yamashita
authors.affiliation: Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan; Japan Science and Technology Agency, CREST, 5 Sanbancho, Chiyoda-ku, Tokyo 102-0075, Japan.
authors.forename: Heiji
authors.initials: H
authors.lastname: Watanabe
mesh_terms: D000327:Adsorption; D000596:Amino Acids / Q000737:chemistry*; D002852:Chromatography, Ion Exchange; D005293:Ferritins / Q000737:chemistry; D008958:Models, Molecular; D010455:Peptides / Q000737:chemistry*; D011136:Polysorbates / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D013057:Spectrum Analysis; D055672:Static Electricity; D013501:Surface-Active Agents / Q000737:chemistry*; D014025:Titanium / Q000737:chemistry*
chemical_list: D000596:Amino Acids; D010455:Peptides; D011136:Polysorbates; D013501:Surface-Active Agents; D012822:Silicon Dioxide; D005293:Ferritins; D014025:Titanium
keywords: Adsorption mechanism; Ferritin; Selective adsorption; Titanium-binding peptide