title: Maternal micronutrients, omega-3 fatty acids, and placental PPARγ expression.
abstract: An altered one-carbon cycle is known to influence placental and fetal development. We hypothesize that deficiency of maternal micronutrients such as folic acid and vitamin B12 will lead to increased oxidative stress, reduced long-chain polyunsaturated fatty acids, and altered expression of peroxisome proliferator activated receptor (PPARγ) in the placenta, and omega-3 fatty acid supplementation to these diets will increase the expression of PPARγ. Female rats were divided into 5 groups: control, folic acid deficient, vitamin B12 deficient, folic acid deficient + omega-3 fatty acid supplemented, and vitamin B12 deficient + omega-3 fatty acid supplemented. Dams were dissected on gestational day 20. Maternal micronutrient deficiency leads to lower (p < 0.05) levels of placental docosahexaenoic acid, arachidonic acid, PPARγ expression and higher (p < 0.05) levels of plasma malonidialdehyde, placental IL-6, and TNF-α. Omega-3 fatty acid supplementation to a vitamin B12 deficient diet normalized the expression of PPARγ and lowered the levels of placental TNF-α. In the case of supplementation to a folic acid deficient diet it lowered the levels of malonidialdehyde and placental IL-6 and TNF-α. This study has implications for fetal growth as oxidative stress, inflammation, and PPARγ are known to play a key role in the placental development.
authors.affiliation: Department of Nutritional Medicine, Interactive Research School for Health Affairs, Bharati Vidyapeeth University, Pune 411043, India.
authors.forename: Akshaya P
authors.initials: AP
authors.lastname: Meher
authors.forename: Asmita A
authors.initials: AA
authors.lastname: Joshi
authors.forename: Sadhana R
authors.initials: SR
authors.lastname: Joshi
mesh_terms: D000818:Animals; D019587:Dietary Supplements; D015525:Fatty Acids, Omega-3 / Q000627:therapeutic use*; D005260:Female; D005494:Folic Acid Deficiency / Q000178:diet therapy* / Q000378:metabolism*; D018977:Micronutrients / Q000172:deficiency*; D047495:PPAR gamma / Q000096:biosynthesis*; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D051381:Rats; D014806:Vitamin B 12 Deficiency / Q000178:diet therapy* / Q000378:metabolism*
chemical_list: D015525:Fatty Acids, Omega-3; D018977:Micronutrients; D047495:PPAR gamma
keywords: IL-6; PPARγ; TNF-α; acide arachidonique; acide docosahexanoïque; acide folique; arachidonic acid; docosahexaenoic acid; folic acid; vitamin B12; vitamine B12