title: [SCF/c-Kit signaling promotes invasion of T24 cells via PI3K pathway].
abstract: OBJECTIVE
  To explore the role of SCF/c-Kit signaling in the invasion of bladder cancer T24 cells.
  
  
  METHODS
  Western blotting was used to detect the expression of c-Kit and PI3K pathway activation stimulated by stem cell factor (SCF) in T24 cells. The invasiveness of T24 cells before and after SCF stimulation and Wortmannin (aspecific PI3K inhibitor) treatment was evaluated using Transwell invasion assay (direct and indirect counting methods).
  
  
  RESULTS
  T24 cells expressed c-Kit protein and showed obvious Akt phosphorylation after stimulation with SCF (1 ng/ml) for 24 h. Compared to the control group, SCF stimulation (1 ng/ml) caused a greater number of T24 cells to migrate through the polycarbonate film (P<0.01), and this effect was blocked by the application of Wortmannin before the stimulation.
  
  
  CONCLUSIONS
  SCF/c-Kit signaling promotes the invasiveness of T24 cells, and this effect is mediated by the PI3K pathway.
authors.affiliation: Department of Immunology/Anhui Provincial Key Laboratory of Infection and Immunity, Bengbu Medical College, Bengbu 233030, China.E-mail: gsj@bbmc.edu.cn.
authors.forename: Shujun
authors.initials: S
authors.lastname: Guo
authors.forename: Xiangnan
authors.initials: X
authors.lastname: Tao
authors.forename: Yimeng
authors.initials: Y
authors.lastname: Wang
authors.forename: Jie
authors.initials: J
authors.lastname: Tang
authors.forename: Lin
authors.initials: L
authors.lastname: Shen
authors.forename: Chuanwang
authors.initials: C
authors.lastname: Song
mesh_terms: D002295:Carcinoma, Transitional Cell / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D006801:Humans; D009361:Neoplasm Invasiveness; D019009:Proto-Oncogene Proteins c-kit / Q000378:metabolism*; D015398:Signal Transduction; D019089:Stem Cell Factor / Q000378:metabolism*; D001749:Urinary Bladder Neoplasms / Q000378:metabolism / Q000473:pathology*
chemical_list: D019089:Stem Cell Factor; D019009:Proto-Oncogene Proteins c-kit