title: Reprogramming somatic cells into pluripotent stem cells using miRNAs.
abstract: Reversal of terminally differentiated somatic cells to ground-state pluripotency has rejuvenated our hopes of generating patient-specific stem cells for therapeutic use in regenerative medicine and drug screening. Originally generated using defined exogenous protein-coding DNA, several methods have been described in reprogramming somatic cells into iPSC. Majority of published methods seek to improve or refine the techniques of reprogramming. This chapter describes reprogramming to pluripotency using miRNAs.
authors.affiliation: Department of Medicine, Perelman School of Medicine, University of Pennsylvania, 3400 Civic Center Boulevard, Bldg 421, Philadelphia, PA, 19104, USA, afred@mail.med.upenn.edu.
authors.forename: Frederick
authors.initials: F
authors.lastname: Anokye-Danso
mesh_terms: D000818:Animals; D065150:Cellular Reprogramming / Q000235:genetics*; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism / Q000821:virology; D057809:HEK293 Cells; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism*; D016086:Lentivirus / Q000235:genetics / Q000502:physiology; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*
chemical_list: D035683:MicroRNAs