title: Beacon-based (bbFISH®) technology for rapid pathogens identification in blood cultures.
abstract: BACKGROUND
  Diagnosis and treatment of bloodstream infections (BSI) are often hampered by the delay in obtaining the final results of blood cultures. Rapid identification of pathogens involved in BSI is of great importance in order to improve survival of septic patients. Beacon-based fluorescent in situ hybridization (hemoFISH® Gram positive and hemoFISH® Gram negative test kits, miacom diagnostics GmbH Düsseldorf, Germany) accelerates the identification of most frequent bacterial pathogens of sepsis.
  
  
  RESULTS
  In this study a total of 558 blood culture (377 blood culture positive and 181 negative) were tested with the hemoFISH® method and the results were evaluated in comparison with the traditional culture based methods. The overall sensitivity and specificity of the hemoFISH® tests were 94.16% and 100%, while, the PPV and NPV were 100 and 89.16%, respectively. As the hemoFISH® results were obtained within 45 mins, the time difference between the final results of the traditional culture method and the hemoFISH® assay was about two days.
  
  
  CONCLUSIONS
  Considering the good sensitivity and specificity of the hemoFISH® assays as well as the significant time saving in obtaining the final results (p-value 0.0001), the introduction of the system could be rialable in the microbiology laboratories, even alongside the traditional systems.
authors.forename: Christina
authors.initials: C
authors.lastname: Sakarikou
authors.forename: Martina
authors.initials: M
authors.lastname: Parisato
authors.forename: Giuliana
authors.initials: G
authors.lastname: Lo Cascio
authors.affiliation: Department of Experimental Medicine and Surgery, "Tor Vergata" University of Rome, Via Montpellier 1, 00133 Rome, Italy. carla.fontana@uniroma2.it.
authors.forename: Carla
authors.initials: C
authors.lastname: Fontana
mesh_terms: D001769:Blood / Q000382:microbiology*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008828:Microbiological Techniques / Q000379:methods*; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D012680:Sensitivity and Specificity; D018805:Sepsis / Q000175:diagnosis*; D013997:Time Factors
references.citation: Am J Clin Pathol. 1996 Jun;105(6):676-88
references.pmid: 8659441
references.citation: Clin Chem. 2011 Jul;57(7):1057-67
references.pmid: 21576270
references.citation: J Clin Microbiol. 2005 Jul;43(7):3390-7
references.pmid: 16000464
references.citation: BMJ Open. 2011 Jun 30;1(1):e000181
references.pmid: 22021785
references.citation: Nature. 2002 Dec 19-26;420(6917):885-91
references.pmid: 12490963
references.citation: Am J Clin Pathol. 2008 Dec;130(6):870-6
references.pmid: 19019762
references.citation: J Clin Microbiol. 2010 May;48(5):1584-91
references.pmid: 20237093
references.citation: Intensive Care Med. 2008 Jan;34(1):17-60
references.pmid: 18058085
references.citation: J Clin Microbiol. 2006 May;44(5):1828-30
references.pmid: 16672413
references.citation: J Clin Microbiol. 2005 Aug;43(8):4070-5
references.pmid: 16081953
references.citation: Arch Pathol Lab Med. 2001 Jul;125(7):863-71
references.pmid: 11419969
references.citation: Clin Microbiol Infect. 2010 Jun;16(6):767-73
references.pmid: 19694763
references.citation: BMJ. 2007 Oct 27;335(7625):879-83
references.pmid: 17962288
references.citation: Int J Antimicrob Agents. 2009;34 Suppl 4:S9-12
references.pmid: 19931822
references.citation: Nat Biotechnol. 1996 Mar;14(3):303-8
references.pmid: 9630890
references.citation: Am J Clin Pathol. 1989 Mar;91(3):331-5
references.pmid: 2923096
references.citation: N Engl J Med. 2003 Jan 9;348(2):138-50
references.pmid: 12519925
references.citation: Med Sci Monit. 2009 Feb;15(2):BR55-60
references.pmid: 19179962
references.citation: BMC Microbiol. 2010 Mar 08;10:72
references.pmid: 20205957
references.citation: J Microbiol Methods. 2000 Jul;41(2):85-112
references.pmid: 10991623
references.citation: J Clin Microbiol. 2003 Jul;41(7):3119-25
references.pmid: 12843051
references.citation: J Clin Pathol. 2007 Jul;60(7):842-3
references.pmid: 17596553
references.citation: Crit Care Med. 2005 Jul;33(7):1538-48
references.pmid: 16003060
references.citation: Crit Care Med. 2006 Jun;34(6):1589-96
references.pmid: 16625125
references.citation: J Bacteriol. 1990 Feb;172(2):762-70
references.pmid: 1688842
references.citation: Chest. 1999 Feb;115(2):462-74
references.pmid: 10027448
references.citation: Clin Biochem Rev. 2007 Nov;28(4):179-94
references.pmid: 18392122
references.citation: Am J Clin Pathol. 1989 Apr;91(4):452-7
references.pmid: 2929500