title: [Expression change and significance of homebox gene-A10 in rat cryptorchidism].
abstract: OBJECTIVE
  To explore the effects of HOXA10 gene expression in undescended and descended testis.
  
  
  METHODS
  Twenty-four male Sprague Dawley rats were surgically prepared for unilateral cryptorchidism model. Their undescended and descended testes were removed at days 7 (infancy period, group B7) and 60 (sexual maturity period, group B60) post-operation. And contralateral descended testis served as controls (group A7, group A60). The expression of HOXA10 with histologic changes in experimental cryptorchidism were detected with one-step real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry.
  
  
  RESULTS
  Compared with the control group, the levels of HOXA10 gene for groups B7 and B60 decreased markedly to 0.78 ± 0.11 (P < 0.01) and 0.56 ± 0.03 (P < 0.01) respectively.However the levels of HOXA10 gene and protein in group B60 were significantly lower than those in group B7 (P < 0.01).
  
  
  CONCLUSIONS
  There is a lower expression of HOXA10 in undescended testes than in normally developed counterparts. And the expression of HOXA10 decreases with the elapsing time of cryptorchidism.
authors.affiliation: Department of Pediatric Surgery, Affiliated Hospital, Nantong University, Nantong 226001, China.
authors.forename: Yuehua
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Pediatric Surgery, Affiliated Hospital, Nantong University, Nantong 226001, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Pediatric Surgery, Affiliated Hospital, Nantong University, Nantong 226001, China.
authors.forename: Jianfei
authors.initials: J
authors.lastname: Huang
authors.affiliation: Department of Pediatric Surgery, Affiliated Hospital, Nantong University, Nantong 226001, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Pediatric Surgery, Affiliated Hospital, Nantong University, Nantong 226001, China.
authors.forename: Qiyou
authors.initials: Q
authors.lastname: Yin
authors.affiliation: Department of Pediatric Surgery, Affiliated Hospital, Nantong University, Nantong 226001, China.
authors.forename: Wenliang
authors.initials: W
authors.lastname: Ge
authors.affiliation: Department of Pediatric Surgery, Affiliated Hospital, Nantong University, Nantong 226001, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of Pediatric Surgery, Affiliated Hospital, Nantong University, Nantong 226001, China. Email: xianhua@ntu.edu.cn.
authors.forename: Hua
authors.initials: H
authors.lastname: Xian
mesh_terms: D000818:Animals; D003456:Cryptorchidism / Q000378:metabolism*; D004195:Disease Models, Animal; D005801:Genes, Homeobox; D000080905:Homeobox A10 Proteins; D018398:Homeodomain Proteins / Q000378:metabolism*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013737:Testis / Q000378:metabolism*
chemical_list: C534680:HOXA10 protein, rat; D000080905:Homeobox A10 Proteins; D018398:Homeodomain Proteins