title: Application of a real-time PCR method for Salmonella spp., Escherichia coli, Staphylococcus aureus and Clostridium perfringens detection in water samples.
abstract: The diagnostic assessment of water sanitary state is based mainly on the cultivation of bacteria retained on membrane filters. However classical microbiology methods have a lot of disadvantages. More and more frequently, rapid detection and identification of pathogens present in water is based on molecular biology techniques. The aim of this study was to determine the effectiveness and usefulness of a real-time PCR method, when compared to the recommended bacteria culture method, in diagnostics of pathogens in water samples. The research concerned the detection and identification of main sanitary indicators of water such as: Salmonella spp., Escherichia coli, Staphylococcus aureus and Clostridium perfringens. The analyses were conducted in water samples contaminated with the reference material (the aforementioned bacteria) and real environmental samples, which were examined for the presence of nucleic acid of: Salmonella spp., E. coli, S. aureus and C. perfringens using a real-time PCR method.
authors.forename: Beata
authors.initials: B
authors.lastname: Rozwadowska
authors.forename: Marta
authors.initials: M
authors.lastname: Albertyńska
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Hudzik
authors.forename: Hubert
authors.initials: H
authors.lastname: Okła
authors.forename: Krzysztof P
authors.initials: KP
authors.lastname: Jasik
authors.forename: Jan
authors.initials: J
authors.lastname: Słodki
mesh_terms: D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification*; D003016:Clostridium perfringens / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012475:Salmonella / Q000235:genetics / Q000302:isolation & purification; D013211:Staphylococcus aureus / Q000235:genetics / Q000302:isolation & purification; D014871:Water Microbiology; D014873:Water Pollutants
chemical_list: D004269:DNA, Bacterial; D014873:Water Pollutants