title: [Expression and regulatory mechanism of microRNA-155 in the villi of patients with unexplained recurrent spontaneous abortion patients].
abstract: OBJECTIVE
  To study the expression and the mechanism of miR-155in the villi of patients with unexplained recurrent spontaneous abortion (URSA).
  
  
  METHODS
  The expression of miR-155 in the villi of 36 cases with URSA (URSA group) and 25 women with normal early pregnancy (control group) were detected by stem-loop real-time reverse transcription (RT) qPCR.Expression of hypoxia inducible factor-1 (HIF-1α), vascular endothelial cell growth factor (VEGF) and micro lymphatic vessel density (MVD) in the villi of were measured by immnohistochemical staining among two groups.
  
  
  RESULTS
  (1)miR-155 expression:the mean miR-155 expression were 1.456 (0.489, 2.459) in URSA group and 2.833 (1.740, 3.794) in control group, which reached statistical difference (P < 0.05). The mean expression of miR-155 of 1.683 (0.902, 2.459) in URSA group with abortion times ( ≤ 3) was significantly higher than 1.229 (0.489, 1.719) in URSA group with more than 4 times abortion (P < 0.05). (2) Indexes :the expression of HIF-1α, VEGF and MVD value were 121 ± 12, 134 ± 12, 36 ± 6 in URSA group and 99 ± 10, 109 ± 10, 28 ± 4 in control group, which reached statistical difference (P < 0.01). The expression of HIF-1α, VEGF and MVD value of 119 ± 12, 134 ± 12, 35 ± 5 in URSA group with less than 3 times abortion was significantly lower than 128 ± 12, 138 ± 12, 43 ± 6 in URSA group with more than 4 times abortion (P < 0.01).
  
  
  CONCLUSIONS
  The expression of miR-155 and HIF-1α is topically stimulated by oxygen signal.HIF-1α adjusts the transcription and translation of VEGF, which together involved in placental trophoblast invasion and placental angiogenesis. The low expression of miR-155 could interfere with expression of HIF-1α and VEGF, which might be involved in villous vascular dysplasia in URSA.
authors.affiliation: Department of Obstetrics and Gynecology, First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Biru
authors.initials: B
authors.lastname: Xiao
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Xue
authors.forename: Feihong
authors.initials: F
authors.lastname: Hu
authors.forename: Rongrong
authors.initials: R
authors.lastname: Sun
authors.forename: Qiuyue
authors.initials: Q
authors.lastname: Chen
authors.forename: Mengmeng
authors.initials: M
authors.lastname: Yang
authors.affiliation: Email: zhwm63@126.com.
authors.forename: Wenmiao
authors.initials: W
authors.lastname: Zhang
mesh_terms: D000026:Abortion, Habitual / Q000378:metabolism* / Q000473:pathology; D000328:Adult; D016022:Case-Control Studies; D002824:Chorionic Villi / Q000098:blood supply / Q000378:metabolism*; D015536:Down-Regulation; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism*; D007150:Immunohistochemistry; D035683:MicroRNAs / Q000378:metabolism*; D008833:Microcirculation; D010920:Placenta / Q000098:blood supply / Q000378:metabolism; D011247:Pregnancy; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; C524942:MIRN155 microRNA, human; D035683:MicroRNAs; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A