title: Comparative plant sphingolipidomic reveals specific lipids in seeds and oil.
abstract: Plant sphingolipids are a highly diverse family of structural and signal lipids. Owing to their chemical diversity and complexity, a powerful analytical method was required to identify and quantify a large number of individual molecules with a high degree of structural accuracy. By using ultra-performance liquid chromatography with a single elution system coupled to electrospray ionization tandem mass spectrometry (UPLC-ESI-MS/MS) in the positive multiple reaction monitoring (MRM) mode, detailed sphingolipid composition was analyzed in various tissues of two Brassicaceae species Arabidopsis thaliana and Camelina sativa. A total of 300 molecular species were identified defining nine classes of sphingolipids, including Cers, hCers, Glcs and GIPCs. High-resolution mass spectrometry identified sphingolipids including amino- and N-acylated-GIPCs. The comparative analysis of seedling, seed and oil sphingolipids showed tissue specific distribution suggesting metabolic channeling and compartmentalization.
authors.affiliation: Institut Jean-Pierre Bourgin, UMR1318, INRA-AgroParisTech, route de Saint-Cyr, 78026 Versailles Cedex, France. Electronic address: Frederique.Tellier@versailles.inra.fr.
authors.forename: Frédérique
authors.initials: F
authors.lastname: Tellier
authors.affiliation: Institut Jean-Pierre Bourgin, UMR1318, INRA-AgroParisTech, route de Saint-Cyr, 78026 Versailles Cedex, France.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Maia-Grondard
authors.affiliation: Centre de recherche de Gif, Institut de Chimie des Substances Naturelles, CNRS, Avenue de la Terrasse, 91198 Gif-sur-Yvette, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Schmitz-Afonso
authors.affiliation: Institut Jean-Pierre Bourgin, UMR1318, INRA-AgroParisTech, route de Saint-Cyr, 78026 Versailles Cedex, France.
authors.forename: Jean-Denis
authors.initials: JD
authors.lastname: Faure
mesh_terms: D002851:Chromatography, High Pressure Liquid; D008055:Lipids / Q000032:analysis* / Q000737:chemistry; D010938:Plant Oils / Q000032:analysis* / Q000737:chemistry; D010944:Plants / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D013107:Sphingolipids / Q000032:analysis* / Q000737:chemistry; D053719:Tandem Mass Spectrometry
chemical_list: D008055:Lipids; D010938:Plant Oils; D013107:Sphingolipids
keywords: Ceramide; GIPC; Mass spectrometry; Oil; Seed; Seedling; Sphingolipid