title: Evaluation of monoclonal antibody-based sandwich direct ELISA (MSD-ELISA) for antigen detection of foot-and-mouth disease virus using clinical samples.
abstract: A monoclonal antibody-based sandwich direct ELISA (MSD-ELISA) method was previously developed for foot-and-mouth disease (FMD) viral antigen detection. Here we evaluated the sensitivity and specificity of two FMD viral antigen detection MSD-ELISAs and compared them with conventional indirect sandwich (IS)-ELISA. The MSD-ELISAs were able to detect the antigen in saliva samples of experimentally-infected pigs for a longer term compared to the IS-ELISA. We also used 178 RT-PCR-positive field samples from cattle and pigs affected by the 2010 type-O FMD outbreak in Japan, and we found that the sensitivities of both MSD-ELISAs were about 7 times higher than that of the IS-ELISA against each sample (P<0.01). In terms of the FMD-positive farm detection rate, the sensitivities of the MSD-ELISAs were about 6 times higher than that of the IS-ELISA against each farm (P<0.01). Although it is necessary to conduct further validation study using the other virus strains, MSD-ELISAs could be appropriate as a method to replace IS-ELISA for FMD antigen detection.
authors.affiliation: Exotic Disease Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization, Josuihoncho Kodaira, Tokyo, Japan.
authors.forename: Kazuki
authors.initials: K
authors.lastname: Morioka
authors.affiliation: Exotic Disease Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization, Josuihoncho Kodaira, Tokyo, Japan.
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Fukai
authors.affiliation: Exotic Disease Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization, Josuihoncho Kodaira, Tokyo, Japan.
authors.forename: Kenichi
authors.initials: K
authors.lastname: Sakamoto
authors.affiliation: Exotic Disease Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization, Josuihoncho Kodaira, Tokyo, Japan.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Yoshida
authors.affiliation: Exotic Disease Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization, Josuihoncho Kodaira, Tokyo, Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Kanno
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology*; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay; D005536:Foot-and-Mouth Disease / Q000175:diagnosis* / Q000821:virology*; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics / Q000276:immunology*; D012463:Saliva / Q000821:virology; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis / Q000821:virology
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral
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