title: Population dynamics of nitrifying bacteria for nitritation achieved in Johannesburg (JHB) process treating municipal wastewater.
abstract: Population dynamic of nitrifying bacteria was investigated for nitrogen removal from municipal wastewater. Nitritation was established with nitrite accumulation ratios above 85%. Quantitative PCR indicated that Nitrospira was dominant nitrite oxidizing bacteria (NOB) and Nitrobacter was few. During nitritation achieving, Nitrobacter was firstly eliminated, along with inhibition of Nitrospira bioactivities, then Nitrospira percentage declined and was finally washed out. Nitritation establishment depended on inhibiting and eliminating of NOB rather than ammonia oxidizing bacteria (AOB) enriching. This is the first study where population dynamics of Nitrobacter and Nitrospira were investigated to reveal mechanism of nitritation in a continuous-flow process. Phylogenetic analysis of AOB indicated that Nitrosomonas-like cluster and Nitrosomonas oligotropha were dominant AOB, accounting for 81.6% of amoA gene clone library. Community structure of AOB was similar to that of complete nitrification system with long hydraulic retention time, but different from that of nitritation reactor with low DO concentration.
authors.affiliation: Key Laboratory of Beijing for Water Environment Recovery, Department of Environmental Engineering, Beijing University of Technology, Beijing 100124, China. Electronic address: zengwei_1@263.net.
authors.forename: Wei
authors.initials: W
authors.lastname: Zeng
authors.affiliation: Key Laboratory of Beijing for Water Environment Recovery, Department of Environmental Engineering, Beijing University of Technology, Beijing 100124, China.
authors.forename: Xinlong
authors.initials: X
authors.lastname: Bai
authors.affiliation: Key Laboratory of Beijing for Water Environment Recovery, Department of Environmental Engineering, Beijing University of Technology, Beijing 100124, China.
authors.forename: Limin
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Beijing for Water Environment Recovery, Department of Environmental Engineering, Beijing University of Technology, Beijing 100124, China.
authors.forename: Anqi
authors.initials: A
authors.lastname: Wang
authors.affiliation: Key Laboratory of Beijing for Water Environment Recovery, Department of Environmental Engineering, Beijing University of Technology, Beijing 100124, China.
authors.forename: Yongzhen
authors.initials: Y
authors.lastname: Peng
mesh_terms: D064751:Ammonium Compounds / Q000302:isolation & purification; D001419:Bacteria / Q000254:growth & development*; D008969:Molecular Sequence Data; D058465:Nitrification; D009573:Nitrites / Q000378:metabolism; D009576:Nitrobacter / Q000254:growth & development; D009584:Nitrogen / Q000302:isolation & purification; D009606:Nitrosomonas / Q000254:growth & development; D010084:Oxidation-Reduction; D010802:Phylogeny; D062065:Wastewater / Q000382:microbiology*; D018508:Water Purification / Q000379:methods*
chemical_list: D064751:Ammonium Compounds; D009573:Nitrites; D009584:Nitrogen; D062065:Wastewater
keywords: Ammonia oxidizing bacteria (AOB); Johannesburg (JHB) process; Municipal wastewater; Nitritation; Nitrite oxidizing bacteria (NOB)