title: Development and characterization of 16 polymorphic microsatellite markers from Taiwan cow-tail fir, Keteleeria davidiana var. formosana (Pinaceae) and cross-species amplification in other Keteleeria taxa.
abstract: BACKGROUND
  Keteleeria davidiana var. formosana (Pinaceae), Taiwan cow-tail fir, is an endangered species listed on the IUCN Red List of Threatened Species and only two populations remain, both on the Taiwan Island. Sixteen polymorphic microsatellite loci were developed in an endangered and endemic gymnosperm species, Keteleeria davidiana var. formosana, and were tested in an additional 6 taxa, K. davidiana var. calcarea, K. davidiana var. chienpeii, K. evelyniana, K. fortunei, K. fortunei var. cyclolepis, and K. pubescens, to evaluate the genetic variation available for conservation management and to reconstruct the phylogeographic patterns of this ancient lineage.
  
  
  RESULTS
  Polymorphic primer sets were developed from K. davidiana var. formosana using the modified AFLP and magnetic bead enrichment method. The number of alleles ranged from 3 to 16, with the observed heterozygosity ranging from 0.28 to 1.00. All of the loci were found to be interspecifically amplifiable.
  
  
  CONCLUSIONS
  These polymorphic and transferable loci will be potentially useful for future studies that will focus on identifying distinct evolutionary units within species and establishing the phylogeographic patterns and the process of speciation among closely related species.
authors.forename: Chin-Shang
authors.initials: CS
authors.lastname: Ho
authors.forename: Huei-Chuan
authors.initials: HC
authors.lastname: Shih
authors.forename: Ho-Yih
authors.initials: HY
authors.lastname: Liu
authors.forename: Shau-Ting
authors.initials: ST
authors.lastname: Chiu
authors.forename: Mei-Hui
authors.initials: MH
authors.lastname: Chen
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Ju
authors.forename: Ya-Zhu
authors.initials: YZ
authors.lastname: Ko
authors.forename: Yu-Shen
authors.initials: YS
authors.lastname: Shih
authors.forename: Chaur-Tzuhn
authors.initials: CT
authors.lastname: Chen
authors.affiliation: Department of Biological Sciences, National Sun Yat-sen University, Kaohsiung 804, Taiwan. twhsu@tesri.gov.tw.
authors.forename: Tsai-Wen
authors.initials: TW
authors.lastname: Hsu
authors.forename: Yu-Chung
authors.initials: YC
authors.lastname: Chiang
mesh_terms: D000483:Alleles; D001483:Base Sequence; D001947:Breeding; D003247:Conservation of Natural Resources; D018744:DNA, Plant / Q000235:genetics*; D056727:Endangered Species; D056426:Genetic Loci; D005819:Genetic Markers; D049810:Genetic Speciation; D006579:Heterozygote; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D010802:Phylogeny; D028201:Pinaceae / Q000145:classification / Q000235:genetics*; D011110:Polymorphism, Genetic; D013624:Taiwan
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers
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