title: KSRP and MicroRNA 145 are negative regulators of lipolysis in white adipose tissue.
abstract: White adipose tissue (WAT) releases fatty acids from stored triacylglycerol for an energy source. Here, we report that targeted deletion of KH-type splicing regulatory protein (KSRP), an RNA-binding protein that regulates gene expression at multiple levels, enhances lipolysis in epididymal WAT (eWAT) because of the upregulation of genes promoting lipolytic activity. Expression of microRNA 145 (miR-145) is decreased because of impaired primary miR-145 processing in Ksrp(-/-) eWAT. We show that miR-145 directly targets and represses Foxo1 and Cgi58, activators of lipolytic activity, and forced expression of miR-145 attenuates lipolysis. This study reveals a novel in vivo function of KSRP in controlling adipose lipolysis through posttranscriptional regulation of miR-145 expression.
authors.affiliation: Department of Biochemistry & Molecular Genetics, University of Alabama at Birmingham, Birmingham, Alabama, USA.
authors.forename: Yi-Yu
authors.initials: YY
authors.lastname: Lin
authors.affiliation: Department of Biochemistry & Molecular Genetics, University of Alabama at Birmingham, Birmingham, Alabama, USA.
authors.forename: Chu-Fang
authors.initials: CF
authors.lastname: Chou
authors.affiliation: Gene Expression Regulation Laboratory, IRCCS AOU San Martino-IST, Genoa, Italy.
authors.forename: Matteo
authors.initials: M
authors.lastname: Giovarelli
authors.affiliation: Gene Expression Regulation Laboratory, IRCCS AOU San Martino-IST, Genoa, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Briata
authors.affiliation: Gene Expression Regulation Laboratory, IRCCS AOU San Martino-IST, Genoa, Italy.
authors.forename: Roberto
authors.initials: R
authors.lastname: Gherzi
authors.affiliation: Department of Biochemistry & Molecular Genetics, University of Alabama at Birmingham, Birmingham, Alabama, USA cchen@uab.edu.
authors.forename: Ching-Yi
authors.initials: CY
authors.lastname: Chen
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology / Q000378:metabolism; D052436:Adipose Tissue, White / Q000378:metabolism*; D050154:Adiposity; D000818:Animals; D002454:Cell Differentiation; D048429:Cell Size; D015536:Down-Regulation / Q000235:genetics; D004822:Epididymis / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D000071161:Forkhead Box Protein O1; D051858:Forkhead Transcription Factors / Q000378:metabolism; D017353:Gene Deletion; D008066:Lipolysis / Q000235:genetics*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009929:Organ Size; D010084:Oxidation-Reduction; D012323:RNA Processing, Post-Transcriptional / Q000235:genetics; D016601:RNA-Binding Proteins / Q000378:metabolism*; D022722:Thermogenesis / Q000235:genetics; D015534:Trans-Activators / Q000172:deficiency / Q000378:metabolism*; D014280:Triglycerides / Q000378:metabolism
chemical_list: D005227:Fatty Acids; D000071161:Forkhead Box Protein O1; D051858:Forkhead Transcription Factors; C467200:Foxo1 protein, mouse; C507913:Khsrp protein, mouse; C543499:MIRN145a microRNA, mouse; D035683:MicroRNAs; D016601:RNA-Binding Proteins; D015534:Trans-Activators; D014280:Triglycerides
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