title: Expression response of duplicated metallothionein 3 gene to copper stress in Silene vulgaris ecotypes.
abstract: Metallothioneins (MTs) were identified as important players in metal metabolism. MT3 gene presents a key metallothionein controlling copper homeostasis in plants. We have selected one cupricolous and one non-cupricolous ecotype to isolate and analyse the MT3 gene in Silene vulgaris. For expression data comparison, we have also included other metal-tolerant ecotypes. Based on a S. vulgaris BAC library screening, we have identified and sequenced a genomic clone containing MT3 gene (SvMT3). We found that SvMT3 gene has been locally duplicated in a tandem arrangement. Expression analysis and complementation studies using yeast mutants showed that both copies of the SvMT3 gene were functional. Moreover, we examined the expression of MT3 gene(s) in selected ecotypes under different copper treatments to show the tissue-specific expression response to copper stress. We demonstrated that higher copper concentrations specifically affected MT3 expression among ecotypes. Our analysis shows that MT3a has similar expression pattern in cupricolous ecotypes while MT3b has common expression features shared by all metallophyte S. vulgaris ecotypes. Our data indicate that down-regulation of MT3b root expression in higher copper concentrations is associated with copper stress. We propose that there might be a specific regulation of SvMT3s transcription depending on the type of heavy metal tolerance.
authors.affiliation: Department of Plant Developmental Genetics, Institute of Biophysics, Academy of Sciences of the Czech Republic, v.v.i., Kralovopolska 135, 612 65, Brno, Czech Republic, nevrtalova@ibp.cz.
authors.forename: Eva
authors.initials: E
authors.lastname: Nevrtalova
authors.forename: Jiri
authors.initials: J
authors.lastname: Baloun
authors.forename: Vojtech
authors.initials: V
authors.lastname: Hudzieczek
authors.forename: Radim
authors.initials: R
authors.lastname: Cegan
authors.forename: Boris
authors.initials: B
authors.lastname: Vyskot
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Dolezel
authors.forename: Jan
authors.initials: J
authors.lastname: Safar
authors.forename: David
authors.initials: D
authors.lastname: Milde
authors.forename: Roman
authors.initials: R
authors.lastname: Hobza
mesh_terms: D000222:Adaptation, Physiological / Q000187:drug effects / Q000235:genetics; D017360:Arabidopsis / Q000235:genetics; D003300:Copper / Q000378:metabolism / Q000633:toxicity*; D060146:Ecotype; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D020131:Genes, Duplicate; D017343:Genes, Plant; D006861:Hydrogen Peroxide / Q000378:metabolism; D000090023:Metallothionein 3; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics; D018517:Plant Roots / Q000033:anatomy & histology / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D060888:Real-Time Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D029751:Silene / Q000235:genetics* / Q000502:physiology*
chemical_list: D000090023:Metallothionein 3; D009419:Nerve Tissue Proteins; D003300:Copper; D006861:Hydrogen Peroxide
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