title: [Methods of testing potential inhibitors of hepatitis C in Huh-7.5 cell line].
abstract: BACKGROUND
  According to WHO reports, there are 130-170 million persons chronically infected with hepatitis C virus on a global scale. There is no effective vaccine against HCV, and the current standard of chronic hepatitis C therapy has limited efficiency and undesirable side effects. Current studies are focused on searching for a new therapeutic agents, which are specifically targeted against the virus. The aim of the study was to develop a methodology for testing the activity and cytotoxicity of potential helicase inhibitors (derivatives of anthracycline antibiotics) in Huh-7.5 cell line infected with HCV.
  
  
  METHODS
  The Huh-7.5 cell line was infected with the JFH1 (Japanese Fulminant Hepatitis) RNA by lipofection. The cytotoxicity of anthracycline antibiotics was measured by Cell Proliferation Kit II(XTT), after 1, 2, 3, 4 and 24 hours after incubation with tetrazolium salt XTT. The activity ofanthracycline antibiotics was examined by Real-Time PCR method.
  
  
  RESULTS
  The study allowed to optimize the conditions of cytotoxicity and activity studies of anthracycline antibiotics.
  
  
  CONCLUSIONS
  Huh-7.5 cell line infected with HCV is a robust cell culture model for screening new antivirals against HCV.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Kołakowska
authors.forename: Paulina
authors.initials: P
authors.lastname: Godzik
authors.forename: Kazimierz
authors.initials: K
authors.lastname: Madaliński
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology*; D002460:Cell Line; D055808:Drug Discovery / Q000379:methods*; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D016174:Hepacivirus / Q000187:drug effects*; D006526:Hepatitis C / Q000188:drug therapy*; D006801:Humans; D020365:RNA Helicases / Q000037:antagonists & inhibitors*
chemical_list: D000998:Antiviral Agents; D020365:RNA Helicases