title: Validated spectrophtometric method for determination of tamsulosin in bulk and pharmaceutical dosage forms.
abstract: In this study a sensitive, simple and accurate spectrophotometric method was suggested for determination of tamsulosin in bulk powder and pharmaceutical dosage form based on the formation of an ion-pair complex between the drug and bromocresol green in a buffer solution at pH 3.5. The formed yellow color complex was extracted with chloroform and measured at 415 nm. The optimum reaction conditions such as pH, reagent amount, extracting solvent and the stoichiometry of the ion-pair complex were investigated. Under the optimized conditions, the Beer's law was obeyed in the concentration range of 1-160 g/mL with acceptable correlation coefficient (r(2) > 0.9997) and precision (CV < 3%) and accuracy (error < 2%). The proposed method was successfully used for the determination of tamsulosin in pharmaceutical capsule with nosignificant interferences of excipients.
authors.forename: Massoud
authors.initials: M
authors.lastname: Amanlou
authors.forename: Amin
authors.initials: A
authors.lastname: Ghazi Moghadam
authors.forename: Maliheh
authors.initials: M
authors.lastname: Barazandeh Tehrani
authors.forename: Effat
authors.initials: E
authors.lastname: Souri
keywords: Bromocresol green; Ion-pair complex; Spectrophotometry; Tamsulosin
references.citation: Methods Enzymol. 1982;87:509-25
references.pmid: 7176926
references.citation: Biomed Chromatogr. 2005 Dec;19(10):709-19
references.pmid: 15828055
references.citation: J Pharm Biomed Anal. 2007 Jan 17;43(2):606-12
references.pmid: 16920322
references.citation: J Pharm Biomed Anal. 2005 Sep 15;39(3-4):691-6
references.pmid: 15935602
references.citation: Pak J Pharm Sci. 2008 Jul;21(3):307-10
references.pmid: 18614430
references.citation: J Chromatogr A. 2003 Feb 14;987(1-2):57-66
references.pmid: 12613797
references.citation: J Chromatogr B Biomed Sci Appl. 1997 Aug 1;695(2):317-27
references.pmid: 9300868
references.citation: J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Oct 5;809(2):307-11
references.pmid: 15315781
references.citation: J Chromatogr B Analyt Technol Biomed Life Sci. 2002 Feb 5;767(1):75-81
references.pmid: 11863298
references.citation: J Pharm Biomed Anal. 2004 Feb 18;34(3):689-93
references.pmid: 15127825
references.citation: Pharm Methods. 2011 Jan;2(1):53-60
references.pmid: 23781431
references.citation: J Pharm Biomed Anal. 2008 Jan 7;46(1):94-103
references.pmid: 17951020