title: mRNA expression of IGF-1 and IGF-1R in patients with colorectal adenocarcinoma and type 2 diabetes.
abstract: OBJECTIVE
  Increasing studies show that messenger RNA (mRNA) levels of local IGF-system are overexpressed in cancer tissue of patients with colorectal cancer (CRC). However, the influence of type 2 diabetes (T2DM) on the expression of insulin-like growth factor-1 (IGF-1) and IGF-1 receptor (IGF-1R) mRNA in colorectal cancer tissue and adjacent non-cancerous tissue (ANCT) is unknown. The aim of this study was to assess mRNA expression of IGF-1 and IGF-1R in paired samples of cancer tissue and ANCT between colorectal adenocarcinoma (CA) patients with and without T2DM.
  
  
  METHODS
  To quantify the levels of IGF-1 and IGF-1R mRNA in CA, we analyzed the expression of IGF-1 and IGF-1R mRNA levels in paired samples of cancer tissue and ANCT in CA patients with and without T2DM using real-time reverse transcription-polymerase chain reaction (RT-PCR).
  
  
  RESULTS
  mRNA levels of IGF-1 and IGF-1R were significantly higher in cancer tissue compared with its ANCT in CA patients with and without T2DM. Compared with the CA group, significantly higher levels of IGF-1 and IGF-1R mRNA were observed in cancer tissue in CA with T2DM group. No significant differences were observed in the role of cancer locations, Dukes stages and diabetes duration on mRNA expression of IGF-1. After adjusting for age, gender and Dukes stages, multivariate analysis indicated IGF-1 mRNA level was a risk factor for prognosis (p <0.05).
  
  
  CONCLUSIONS
  Our results support the hypothesis that IGF system plays an important role in CRC. Further larger studies are needed.
authors.affiliation: Department of Clinical Laboratory, Tianjin Union Medical Center, Tianjin, PR China. Electronic address: 13752062169@126.com.
authors.forename: Rui
authors.initials: R
authors.lastname: Liu
authors.affiliation: Department of Clinical Laboratory, Tianjin Union Medical Center, Tianjin, PR China.
authors.forename: Li-Ling
authors.initials: LL
authors.lastname: Hu
authors.affiliation: Department of Clinical Laboratory, Tianjin Union Medical Center, Tianjin, PR China.
authors.forename: Ao
authors.initials: A
authors.lastname: Sun
authors.affiliation: Department of Pathology, Tianjin Union Medical Center, Tianjin, PR China.
authors.forename: Ya-Jing
authors.initials: YJ
authors.lastname: Cao
authors.affiliation: Department of Pathology, Tianjin Union Medical Center, Tianjin, PR China.
authors.forename: Tao
authors.initials: T
authors.lastname: Tang
authors.affiliation: Department of Colorectal Surgery, Tianjin Union Medical Center, Tianjin, PR China.
authors.forename: Xi-Peng
authors.initials: XP
authors.lastname: Zhang
authors.affiliation: Department of Colorectal Surgery, Tianjin Union Medical Center, Tianjin, PR China.
authors.forename: Qing-Huai
authors.initials: QH
authors.lastname: Zhang
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D000368:Aged; D000369:Aged, 80 and over; D015179:Colorectal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics / Q000378:metabolism* / Q000401:mortality; D005260:Female; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D017526:Receptor, IGF Type 1 / Q000096:biosynthesis* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016019:Survival Analysis
chemical_list: C583557:IGF1 protein, human; D012333:RNA, Messenger; D007334:Insulin-Like Growth Factor I; D017526:Receptor, IGF Type 1
keywords: Colorectal adenocarcinoma; Insulin-like growth factor-1; Insulin-like growth factor-1 receptor; Messenger RNA; Type 2 diabetes