title: Penetrability of AH plus and MTA fillapex after endodontic treatment and retreatment: a confocal laser scanning microscopy study.
abstract: The aim of the study was to assess the penetrability of two endodontic sealers (AH Plus and MTA Fillapex) into dentinal tubules, submitted to endodontic treatment and subsequently to endodontic retreatment. Thirty ex vivo incisors were prepared using ProTaper rotary system up to F3 instrument and divided in three groups according to the endodontic sealer used for root canal filling: AH Plus (AHP), MTA Fillapex (MTAF), and control group (CG) without using EDTA previously to the root canal filling. Rhodamine B dye (red) was incorporated to the sealers in order to provide the fluorescence which will enable confocal laser scanning microscopy (CLSM) assessment. All specimens were filled with gutta-percha cones using the lateral compaction technique. The specimens were submitted to endodontic retreatment using ProTaper Retreatment system, re-prepared up to F5 instruments and filled with gutta-percha cones and the same sealer used during endodontic retreatment. Fluorescein dye (green) was incorporated to the sealer in order to distinguish from the first filling. The roots were sectioned 2 mm from the apex and assessed by CLSM. No difference was found between the two experimental groups (P > 0.05). On the other hand, in the control group the sealers were not capable to penetrate into dentinal tubules after endodontic treatment (P > 0.05). In retreatment cases, none of the sealers were able to penetrate into dentin tubules. It can be concluded that sealer penetrability is high during endodontic treatment. However, MTA Fillapex and AH Plus do not penetrate into dentinal tubules after endodontic retreatment.
authors.affiliation: Private Practioner, Porto Alegre, RS, Brazil.
authors.forename: Daniela
authors.initials: D
authors.lastname: Kok
authors.forename: Ricardo Abreu da
authors.initials: RA
authors.lastname: Rosa
authors.forename: Mirela Sangoi
authors.initials: MS
authors.lastname: Barreto
authors.forename: Fernanda Hoffmann
authors.initials: FH
authors.lastname: Busanello
authors.forename: Manuela Favarin
authors.initials: MF
authors.lastname: Santini
authors.forename: Jefferson Ricardo
authors.initials: JR
authors.lastname: Pereira
authors.forename: Marcus Vinícius Reis
authors.initials: MV
authors.lastname: Só
mesh_terms: D017607:Aluminum Compounds / Q000627:therapeutic use*; D017610:Calcium Compounds / Q000627:therapeutic use*; D004338:Drug Combinations; D004853:Epoxy Resins / Q000627:therapeutic use*; D006801:Humans; D018613:Microscopy, Confocal; D010087:Oxides / Q000627:therapeutic use*; D019233:Retreatment / Q000379:methods; D012387:Root Canal Filling Materials / Q000627:therapeutic use*; D012390:Root Canal Therapy / Q000379:methods*; D017640:Silicates / Q000627:therapeutic use*; D016896:Treatment Outcome
chemical_list: D017607:Aluminum Compounds; D017610:Calcium Compounds; D004338:Drug Combinations; D004853:Epoxy Resins; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; C534916:epoxy resin-based root canal sealer; C086631:mineral trioxide aggregate
keywords: confocal laser scanning microscopy; endodontics; retreatment; root canal filling