title: A novel in vitro co-culture system allows the concurrent analysis of mature biofilm and planktonic bacteria with human lung epithelia.
abstract: Pseudomonas aeruginosa establishes chronic infections by forming biofilms; however studies of the virulence have focused on the planktonic form. Few in vitro co-culture models exist to study biofilm infections. We present a novel in vitro co-culture method examining the interactions between mature P. aeruginosa biofilms and human lung epithelial cells.
authors.affiliation: University of Manitoba, Winnipeg, Manitoba, Canada; Cangene Corporation, Winnipeg, Manitoba, Canada. Electronic address: lbowler@cangene.com.
authors.forename: Laura L
authors.initials: LL
authors.lastname: Bowler
authors.affiliation: University of Manitoba, Winnipeg, Manitoba, Canada; National HIV and Retrovirology Laboratories, Public Health Agency of Canada, Canada.
authors.forename: T Blake
authors.initials: TB
authors.lastname: Ball
authors.affiliation: University of Manitoba, Winnipeg, Manitoba, Canada; Cangene Corporation, Winnipeg, Manitoba, Canada.
authors.forename: Laura L
authors.initials: LL
authors.lastname: Saward
mesh_terms: D017209:Apoptosis; D001422:Bacterial Adhesion; D018441:Biofilms / Q000254:growth & development*; D018920:Coculture Techniques / Q000379:methods*; D006801:Humans; D016209:Interleukin-8; D008954:Models, Biological; D010933:Plankton / Q000502:physiology*; D020545:Respiratory Mucosa / Q000166:cytology* / Q000382:microbiology*
chemical_list: D016209:Interleukin-8
keywords: Chronic lung infection; Co-culture; Human lung epithelia; Innate immunity; Mature biofilm; Pseudomonas aeruginosa