title: [Mutational analysis of MYO1E in Chinese children with familial steroid-resistant nephrotic syndrome].
abstract: OBJECTIVE
  Steroid-resistant nephrotic syndrome (SRNS) with MYO1E mutations has been identified as autosomal recessive focal segmental glomerulosclerosis (FSGS). To date, only two homozygous mutations in the MYO1E gene were reported in three families with FSGS. This study aimed to examine mutations in the MYO1E gene in children with familial SRNS in the Han Chinese ethnic group.
  
  
  METHODS
  Between 2005 and 2010, peripheral blood samples were collected from the probands, their siblings and parents of four families with autosomal recessive SRNS in the Han Chinese ethnic group. Four probands were studied from nine patients. The mutational analysis of MYO1E was performed by polymerase chain reaction and direct DNA sequencing. Fifty-nine healthy volunteers with normal urine analysis were included as controls.
  
  
  RESULTS
  Twenty-five MYO1E variants in the prohands from 4 families with SRNS were identified in this study. Among them, 24 variants were found in NCBI dbSNP. One heterozygous mutation IVS21-85G>A was found in the prohand from Family D, whereas it was absent in 59 normal Chinese controls. No splice site change caused by IVS21-85G>A was reported by analysis with NetGene2.
  
  
  CONCLUSIONS
  MYO1E mutations are not a major cause of Chinese familial SRNS in this study.
authors.affiliation: Department of Pediatrics, Fuzhou General Hospital, Nanjing Command, Fuzhou 350025, China. zihuayu@vip.sina.com.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhao
authors.forename: Zi-Hua
authors.initials: ZH
authors.lastname: Yu
authors.forename: Yong-Hui
authors.initials: YH
authors.lastname: Yang
authors.forename: Xiao-Jing
authors.initials: XJ
authors.lastname: Nie
authors.forename: Jun
authors.initials: J
authors.lastname: Huang
authors.forename: Cheng-Feng
authors.initials: CF
authors.lastname: Wang
authors.forename: Gui-Zhi
authors.initials: GZ
authors.lastname: Xia
authors.forename: Guang-Ming
authors.initials: GM
authors.lastname: Chen
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D002681:China / Q000208:ethnology; D004252:DNA Mutational Analysis; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D009154:Mutation; D024461:Myosin Type I / Q000235:genetics*; D009404:Nephrotic Syndrome / Q000151:congenital* / Q000235:genetics
chemical_list: C511011:MYO1E protein, human; D024461:Myosin Type I