title: Exploratory study of oral mucosal colonization of human gastric Helicobacter pylori in mice.
abstract: In this study, human gastric Helicobacter pylori (Hp) was closely attached to the pre-treated mouse buccal mucosa by using artificial oral film to induce the growth and colonization of Hp on the buccal mucosa in mice. Sixty BALB/c mice were divided into three groups, in which Hp biofilm colonization was detected in three mice in Hp film group (Hp mesh biofilm accumulation under an optical microscope; Hp accumulated colonization under an electron microscope). There were no Hp biofilms detected in Hp smear group or the control group with black film. In this study, human gastric Hp was first used to artificially induce the growth and colonization of Hp on the buccal mucosa in mice. The mouse model of oral infection with Hp was initially established, providing animal experimental evidences for oral conditions of growth and colonization of Hp on the buccal mucosa in mice, and providing a workable animal modeling method for further research of joint infection of Hp on the mouth and stomach, as well as the relationship between oral Hp and gastric Hp.
authors.affiliation: Department of Pathogenic Microbiology, Foshan University Foshan, Guangdong, 528000, China.
authors.forename: Xueqin
authors.initials: X
authors.lastname: Wan
authors.affiliation: Department of Pathogenic Microbiology, Foshan University Foshan, Guangdong, 528000, China.
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Tang
authors.affiliation: Guanghua School of Stomatology, Sun Yat-Sen University Guangzhou, Guangdong, 510000, China.
authors.forename: Xiaohuan
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Pathogenic Microbiology, Foshan University Foshan, Guangdong, 528000, China.
authors.forename: Hongming
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Pathogenic Microbiology, Foshan University Foshan, Guangdong, 528000, China.
authors.forename: Zhixin
authors.initials: Z
authors.lastname: Cui
authors.affiliation: Department of Gastroenterology, Xijing Hospital Xi'an, 710032, China.
authors.forename: Sijuan
authors.initials: S
authors.lastname: Hu
authors.affiliation: Department of Pathogenic Microbiology, Foshan University Foshan, Guangdong, 528000, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Huang
keywords: Helicobacter pylori; mouse model; oral colonization
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