title: The Polyphenol Chlorogenic Acid Attenuates UVB-mediated Oxidative Stress in Human HaCaT Keratinocytes.
abstract: We investigated the protective effects of chlorogenic acid (CGA), a polyphenol compound, on oxidative damage induced by UVB exposure on human HaCaT cells. In a cell-free system, CGA scavenged 1,1-diphenyl-2-picrylhydrazyl radicals, superoxide anions, hydroxyl radicals, and intracellular reactive oxygen species (ROS) generated by hydrogen peroxide and ultraviolet B (UVB). Furthermore, CGA absorbed electromagnetic radiation in the UVB range (280-320 nm). UVB exposure resulted in damage to cellular DNA, as demonstrated in a comet assay; pre-treatment of cells with CGA prior to UVB irradiation prevented DNA damage and increased cell viability. Furthermore, CGA pre-treatment prevented or ameliorated apoptosis-related changes in UVB-exposed cells, including the formation of apoptotic bodies, disruption of mitochondrial membrane potential, and alterations in the levels of the apoptosis-related proteins Bcl-2, Bax, and caspase-3. Our findings suggest that CGA protects cells from oxidative stress induced by UVB radiation.
authors.affiliation: School of Medicine and Institute for Nuclear Science and Technology, Jeju National University, Jeju 690-756, Republic of Korea.
authors.forename: Ji Won
authors.initials: JW
authors.lastname: Cha
authors.affiliation: School of Medicine and Institute for Nuclear Science and Technology, Jeju National University, Jeju 690-756, Republic of Korea.
authors.forename: Mei Jing
authors.initials: MJ
authors.lastname: Piao
authors.affiliation: School of Medicine and Institute for Nuclear Science and Technology, Jeju National University, Jeju 690-756, Republic of Korea.
authors.forename: Ki Cheon
authors.initials: KC
authors.lastname: Kim
authors.affiliation: School of Medicine and Institute for Nuclear Science and Technology, Jeju National University, Jeju 690-756, Republic of Korea.
authors.forename: Cheng Wen
authors.initials: CW
authors.lastname: Yao
authors.affiliation: School of Medicine and Institute for Nuclear Science and Technology, Jeju National University, Jeju 690-756, Republic of Korea.
authors.forename: Jian
authors.initials: J
authors.lastname: Zheng
authors.affiliation: School of Medicine and Institute for Nuclear Science and Technology, Jeju National University, Jeju 690-756, Republic of Korea.
authors.forename: Seong Min
authors.initials: SM
authors.lastname: Kim
authors.affiliation: School of Medicine and Institute for Nuclear Science and Technology, Jeju National University, Jeju 690-756, Republic of Korea.
authors.forename: Chang Lim
authors.initials: CL
authors.lastname: Hyun
authors.affiliation: Research Institute of Processing from Jeju Fisher Food, Choung Ryong Fisheries Co., LTD, Jeju 697-943, Republic of Korea.
authors.forename: Yong Seok
authors.initials: YS
authors.lastname: Ahn
authors.affiliation: School of Medicine and Institute for Nuclear Science and Technology, Jeju National University, Jeju 690-756, Republic of Korea.
authors.forename: Jin Won
authors.initials: JW
authors.lastname: Hyun
keywords: Apoptosis; Chlorogenic acid; Human keratinocyte; Oxidative stress; Ultraviolet B
references.citation: Free Radic Biol Med. 1996;20(7):933-56
references.pmid: 8743980
references.citation: Chem Pharm Bull (Tokyo). 2011;59(6):793-6
references.pmid: 21628922
references.citation: Biomol Ther (Seoul). 2013 Nov;21(6):470-5
references.pmid: 24404338
references.citation: J Immunol Methods. 1992 Nov 25;156(1):39-45
references.pmid: 1431161
references.citation: Toxicol Appl Pharmacol. 2004 Mar 15;195(3):298-308
references.pmid: 15020192
references.citation: Int J Dermatol. 2010 Sep;49(9):978-86
references.pmid: 20883261
references.citation: Mol Carcinog. 1999 Mar;24(3):169-76
references.pmid: 10204801
references.citation: Photochem Photobiol Sci. 2013 Jan;12(1):54-64
references.pmid: 23111621
references.citation: J Agric Food Chem. 2008 Apr 23;56(8):2660-7
references.pmid: 18348529
references.citation: Biochim Biophys Acta. 1998 Aug 10;1366(1-2):151-65
references.pmid: 9714783
references.citation: Science. 1998 Aug 28;281(5381):1309-12
references.pmid: 9721092
references.citation: J Cosmet Sci. 2013 May-Jun;64(3):207-17
references.pmid: 23752035
references.citation: J Zhejiang Univ Sci B. 2007 Sep;8(9):673-9
references.pmid: 17726749
references.citation: Nat Protoc. 2007;2(11):2719-27
references.pmid: 18007607
references.citation: Cancer Res. 1997 Aug 15;57(16):3554-61
references.pmid: 9270028
references.citation: Radiat Environ Biophys. 2007 Mar;46(1):61-8
references.pmid: 17279358
references.citation: Mutat Res. 2005 Apr 1;571(1-2):153-73
references.pmid: 15748645
references.citation: Mutat Res. 2003 Apr 20;536(1-2):15-25
references.pmid: 12694742
references.citation: Int J Pharm. 2008 Nov 3;363(1-2):183-91
references.pmid: 18718515
references.citation: Photochem Photobiol Sci. 2013 Oct;12(10):1830-40
references.pmid: 23896761
references.citation: Proc Natl Acad Sci U S A. 1993 Sep 1;90(17):7915-22
references.pmid: 8367443
references.citation: Food Chem Toxicol. 2013 Oct;60:123-9
references.pmid: 23871788
references.citation: Eur J Pharmacol. 2012 Jan 15;674(2-3):65-72
references.pmid: 21749866
references.citation: Mutat Res. 2005 Apr 1;571(1-2):107-20
references.pmid: 15748642
references.citation: Z Lebensm Unters Forsch. 1996 Apr;202(4):299-302
references.pmid: 8638432
references.citation: Int J Biochem Cell Biol. 2007;39(1):44-84
references.pmid: 16978905
references.citation: J Exp Med. 1996 Apr 1;183(4):1533-44
references.pmid: 8666911
references.citation: Nat Rev Cancer. 2004 Jan;4(1):23-35
references.pmid: 14681688
references.citation: J Exp Med. 1995 Aug 1;182(2):367-77
references.pmid: 7629499
references.citation: Carcinogenesis. 2003 May;24(5):927-36
references.pmid: 12771038
references.citation: Photochem Photobiol. 2004 Feb;79(2):193-8
references.pmid: 15068032
references.citation: J Photochem Photobiol B. 2013 Oct 5;127:153-60
references.pmid: 24041853
references.citation: J Invest Dermatol. 2001 Jun;116(6):860-6
references.pmid: 11407972
references.citation: J Nutr. 2003 Mar;133 Suppl 3:933S-940S
references.pmid: 12612179
references.citation: Free Radic Res. 1999 Feb;30(2):153-62
references.pmid: 10193583
references.citation: Biochem J. 1999 Sep 15;342 Pt 3:481-96
references.pmid: 10477257
references.citation: Anal Sci. 2003 Jul;19(7):1083-4
references.pmid: 12880098
references.citation: J Biol Chem. 2005 Jul 29;280(30):27888-95
references.pmid: 15944151
references.citation: Antioxid Redox Signal. 2007 Jan;9(1):49-89
references.pmid: 17115887
references.citation: Mutat Res. 2000 Nov 20;455(1-2):111-27
references.pmid: 11113471
references.citation: Photodermatol Photoimmunol Photomed. 2003 Apr;19(2):56-72
references.pmid: 12945805
references.citation: Int J Mol Sci. 2012 Dec 27;14(1):532-46
references.pmid: 23271369
references.citation: Cancer Res. 1987 Feb 15;47(4):936-42
references.pmid: 3802100
references.citation: Pharmacognosy Res. 2012 Jul;4(3):148-53
references.pmid: 22923952