title: Excess α-tocopherol decreases extrahepatic phylloquinone in phylloquinone-fed rats but not menaquinone-4 in menaquinone-4-fed rats.
abstract: METHODS
  The effects of vitamin E on vitamin K metabolism were elucidated by comparing the effect of tocopherol intake on vitamin K concentrations in rats fed phylloquinone (PK) or menaquinone (MK)-4.
  
  
  RESULTS
  Initially, the dietary effect of RRR-α-tocopherol, but not RRR-γ-tocopherol, in decreasing extrahepatic PK concentrations was confirmed. Subsequently, rats were fed a PK or MK-4-containing diet (0.75 mg/kg) with RRR-α-tocopherol (0, 10, 50, or 500 mg/kg) for 6 weeks. In rats fed PK, α-tocopherol consumption decreased PK in kidney, lung, heart, muscle, testis, and brain but not in serum and liver. However, in rats fed MK-4, α-tocopherol consumption did not decrease MK-4 in serum and tissues. Finally, vitamin K- and E-depleted rats were administered PK or MK-4 (0.2 mg) with RRR-α-tocopherol (0, 1, or 10 mg) by gavage. After PK administration, α-tocopherol was observed to decrease PK in kidney, adrenal gland, lung, testis, and brain but not in serum and liver, whereas, after MK-4 administration, α-tocopherol did not affect MK-4 in serum and tissues.
  
  
  CONCLUSIONS
  Excess α-tocopherol decreased extrahepatic PK in rats fed PK but not MK-4 in rats fed MK-4.
authors.affiliation: Department of Nutritional Sciences, Nagoya University of Arts and Sciences, Nisshin, Japan.
authors.forename: Fumiaki
authors.initials: F
authors.lastname: Hanzawa
authors.forename: Eri
authors.initials: E
authors.lastname: Sakuma
authors.forename: Saki
authors.initials: S
authors.lastname: Nomura
authors.forename: Tomono
authors.initials: T
authors.lastname: Uchida
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Oda
authors.forename: Saiko
authors.initials: S
authors.lastname: Ikeda
mesh_terms: D000818:Animals; D019587:Dietary Supplements; D015536:Down-Regulation; D008297:Male; D009928:Organ Specificity; D017208:Rats, Wistar; D013047:Specific Pathogen-Free Organisms; D014811:Vitamin E Deficiency / Q000097:blood / Q000139:chemically induced / Q000178:diet therapy / Q000378:metabolism; D010837:Vitamin K 1 / Q000008:administration & dosage / Q000037:antagonists & inhibitors* / Q000378:metabolism / Q000627:therapeutic use; D024482:Vitamin K 2 / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000097:blood / Q000378:metabolism / Q000627:therapeutic use; D014813:Vitamin K Deficiency / Q000097:blood / Q000139:chemically induced* / Q000178:diet therapy / Q000378:metabolism; D024502:alpha-Tocopherol / Q000008:administration & dosage / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000506:poisoning*; D024504:gamma-Tocopherol / Q000008:administration & dosage / Q000378:metabolism
chemical_list: D024482:Vitamin K 2; C030814:menatetrenone; D010837:Vitamin K 1; D024504:gamma-Tocopherol; D024502:alpha-Tocopherol
keywords: Menaquinone-4; Phylloquinone; Tocopherol; Vitamin E; Vitamin K