title: Intracerebroventricular administration of nerve growth factor induces gliogenesis in sensory ganglia, dorsal root, and within the dorsal root entry zone.
abstract: Previous studies indicated that intracerebroventricular administration of nerve growth factor (NGF) leads to massive Schwann cell hyperplasia surrounding the medulla oblongata and spinal cord. This study was designed to characterize the proliferation of peripheral glial cells, that is, Schwann and satellite cells, in the trigeminal ganglia and dorsal root ganglia (DRG) of adult rats during two weeks of NGF infusion using bromodeoxyuridine (BrdU) to label dividing cells. The trigeminal ganglia as well as the cervical and lumbar DRG were analyzed. Along the entire neuraxis a small number of dividing cells were observed within these regions under physiological condition. NGF infusion has dramatically increased the generation of new cells in the neuronal soma and axonal compartments of sensory ganglia and along the dorsal root and the dorsal root entry zone. Quantification of BrdU positive cells within sensory ganglia revealed a 2.3- to 3-fold increase in glial cells compared to controls with a similar response to NGF for the different peripheral ganglia examined. Immunofluorescent labeling with S100β revealed that Schwann and satellite cells underwent mitosis after NGF administration. These data indicate that intracerebroventricular NGF infusion significantly induces gliogenesis in trigeminal ganglia and the spinal sensory ganglia and along the dorsal root entry zone as well as the dorsal root.
authors.affiliation: Department of Molecular Neurology, Friedrich-Alexander-University Erlangen-Nürnberg (FAU), University Hospital Erlangen, Schwabachanlage 6, 91054 Erlangen, Germany ; Department of Neurosciences, University of California, La Jolla, San Diego, CA 92093-9157, USA.
authors.forename: Johannes C M
authors.initials: JC
authors.lastname: Schlachetzki
authors.affiliation: Department of Neurosciences, University of California, La Jolla, San Diego, CA 92093-9157, USA ; Neurology Service, Veterans Affairs Medical Center, San Diego, CA 92161, USA.
authors.forename: Donald P
authors.initials: DP
authors.lastname: Pizzo
authors.affiliation: Department of Neurosciences, University of California, La Jolla, San Diego, CA 92093-9157, USA.
authors.forename: Debbi A
authors.initials: DA
authors.lastname: Morrissette
authors.affiliation: Department of Molecular Neurology, Friedrich-Alexander-University Erlangen-Nürnberg (FAU), University Hospital Erlangen, Schwabachanlage 6, 91054 Erlangen, Germany ; Department of Neurosciences, University of California, La Jolla, San Diego, CA 92093-9157, USA.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Winkler
mesh_terms: D000818:Animals; D001369:Axons / Q000187:drug effects; D017950:Ganglia, Sensory / Q000187:drug effects / Q000254:growth & development*; D057967:Infusions, Intraventricular; D020932:Nerve Growth Factor / Q000008:administration & dosage*; D009457:Neuroglia / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D013116:Spinal Cord / Q000187:drug effects / Q000254:growth & development; D013126:Spinal Nerve Roots / Q000187:drug effects / Q000254:growth & development*
chemical_list: D020932:Nerve Growth Factor
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