title: Detection of Integrase Gene in E. coli Isolated from Pigs at Different Stages of Production System.
abstract: Integrons are one of the genetic elements involved in the acquisition of antibiotic resistance. The aim of the present research is to investigate the presence of integrons in commensal Escherichia coli (E. coli) strains, isolated from pigs at different stages of production system and from the environment in an Argentinian farm. Five sows postpartum and five randomly chosen piglets from each litter were sampled by rectal swabs. They were sampled again at day 21 and at day 70. Environmental samples from the farm were also obtained. E. coli containing any integron class or combination of both integrons was detected by polymerase chain reaction in 100% of sows and in piglets at different stages of production: farrowing pen stage 68.1%;, weaning 60%, and growing/finishing 85.8%, showing an increase along the production system. From environmental samples 78.4% of E. coli containing any integron class was detected. We conclude that animals and farm environment can act as reservoirs for potential spread of resistant bacteria by means of mobile genetic elements as integrons, which has a major impact on production of food animals and that can reach man through the food chain, constituting a problem for public health.
authors.affiliation: Área de Toxicología, Departamento de Fisiopatología, Centro de Investigación Veterinaria de Tandil-Consejo Nacional de Investigaciones Científicas y Técnicas-Comisión de Investigaciones Científicas Provincia de Buenos Aires (CIVETAN-CONICET-CICPBA), Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires (UNICEN), Campus Universitario, Paraje Arroyo Seco s/n, Tandil, Argentina.
authors.forename: Eulalia
authors.identifier: 0000-0002-8729-7999
authors.initials: E
authors.lastname: de la Torre
authors.affiliation: Área de Inmunoquímica y Biotecnología, Departamento de Sanidad Animal y Medicina Preventiva (SAMP), CIVETAN-CONICET-CICPBA, Facultad de Ciencias Veterinarias, UNICEN, Campus Universitario, Paraje Arroyo Seco s/n, Tandil, Argentina.
authors.forename: Rocío
authors.initials: R
authors.lastname: Colello
authors.affiliation: Área de Inmunoquímica y Biotecnología, Departamento de Sanidad Animal y Medicina Preventiva (SAMP), CIVETAN-CONICET-CICPBA, Facultad de Ciencias Veterinarias, UNICEN, Campus Universitario, Paraje Arroyo Seco s/n, Tandil, Argentina.
authors.forename: Nora Lía
authors.initials: NL
authors.lastname: Padola
authors.affiliation: Área de Inmunoquímica y Biotecnología, Departamento de Sanidad Animal y Medicina Preventiva (SAMP), CIVETAN-CONICET-CICPBA, Facultad de Ciencias Veterinarias, UNICEN, Campus Universitario, Paraje Arroyo Seco s/n, Tandil, Argentina.
authors.forename: Analía
authors.initials: A
authors.lastname: Etcheverría
authors.affiliation: Área de Bioestadística, Departamento de SAMP, CIVETAN-CONICET-CICPBA, Facultad de Ciencias Veterinarias, UNICEN, Campus Universitario, Paraje Arroyo Seco s/n, Tandil, Argentina.
authors.forename: Edgardo
authors.initials: E
authors.lastname: Rodríguez
authors.affiliation: Área de Producción Porcina, Departamento de Producción Animal, Facultad de Ciencias Veterinarias, UNICEN, Campus Universitario, Paraje Arroyo Seco s/n, Tandil, Argentina.
authors.forename: Fabián
authors.initials: F
authors.lastname: Amanto
authors.affiliation: Área de Toxicología, Departamento de Fisiopatología, Centro de Investigación Veterinaria de Tandil-Consejo Nacional de Investigaciones Científicas y Técnicas-Comisión de Investigaciones Científicas Provincia de Buenos Aires (CIVETAN-CONICET-CICPBA), Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires (UNICEN), Campus Universitario, Paraje Arroyo Seco s/n, Tandil, Argentina.
authors.forename: María Ofelia
authors.initials: MO
authors.lastname: Tapia
authors.affiliation: Área de Toxicología, Departamento de Fisiopatología, Centro de Investigación Veterinaria de Tandil-Consejo Nacional de Investigaciones Científicas y Técnicas-Comisión de Investigaciones Científicas Provincia de Buenos Aires (CIVETAN-CONICET-CICPBA), Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires (UNICEN), Campus Universitario, Paraje Arroyo Seco s/n, Tandil, Argentina.
authors.forename: Alejandro Luis
authors.initials: AL
authors.lastname: Soraci
references.citation: Clin Microbiol Infect. 2004 Apr;10(4):272-88
references.pmid: 15059115
references.citation: Appl Environ Microbiol. 2011 Jan;77(2):684-7
references.pmid: 21097590
references.citation: Acta Vet Scand Suppl. 2000;93:47-50; discussion 51-2
references.pmid: 10822857
references.citation: Nat Rev Microbiol. 2010 Aug;8(8):552-63
references.pmid: 20601965
references.citation: FEMS Microbiol Lett. 2008 Jan;278(2):193-9
references.pmid: 18053066
references.citation: Microb Drug Resist. 2009 Sep;15(3):229-37
references.pmid: 19728783
references.citation: J Vet Med B Infect Dis Vet Public Health. 2004 Oct-Nov;51(8-9):374-9
references.pmid: 15525369
references.citation: Annu Rev Genet. 2010;44:141-66
references.pmid: 20707672
references.citation: J Anim Sci. 2001 Oct;79(10):2542-9
references.pmid: 11721832
references.citation: Rev Argent Microbiol. 2010 Jan-Feb;42(1):63-78
references.pmid: 20461298
references.citation: Appl Environ Microbiol. 2011 Oct;77(20):7104-12
references.pmid: 21856835
references.citation: Int J Antimicrob Agents. 2006 May;27(5):383-91
references.pmid: 16621462
references.citation: J Appl Microbiol. 2002;92 Suppl:85S-9S
references.pmid: 12000617
references.citation: Appl Environ Microbiol. 2013 Feb;79(3):853-9
references.pmid: 23160136
references.citation: Front Microbiol. 2012 Apr 09;3:119
references.pmid: 22509175
references.citation: J Clin Microbiol. 2005 Jul;43(7):3074-82
references.pmid: 16000417
references.citation: J Antimicrob Chemother. 2003 Sep;52(3):489-92
references.pmid: 12888584
references.citation: J Antimicrob Chemother. 2006 Jul;58(1):101-7
references.pmid: 16709594
references.citation: Drug Resist Updat. 2000 Oct;3(5):303-311
references.pmid: 11498398
references.citation: Antimicrob Agents Chemother. 2002 Dec;46(12):3963-70
references.pmid: 12435702
references.citation: Environ Pollut. 2002;120(2):475-92
references.pmid: 12395861
references.citation: Microbiol Immunol. 2007;51(5):493-9
references.pmid: 17579258
references.citation: J Antimicrob Chemother. 2008 Aug;62(2):296-302
references.pmid: 18456652
references.citation: PLoS One. 2011 Mar 28;6(3):e17943
references.pmid: 21464930
references.citation: J Theor Biol. 2009 Feb 21;256(4):561-73
references.pmid: 19022263
references.citation: Lett Appl Microbiol. 1998 Dec;27(6):369-71
references.pmid: 9871356
references.citation: J Appl Microbiol. 2003;95(3):428-36
references.pmid: 12911689
references.citation: Emerg Infect Dis. 2001 Mar-Apr;7(2):286-92
references.pmid: 11294725
references.citation: Foodborne Pathog Dis. 2009 Oct;6(8):959-64
references.pmid: 19630513
references.citation: Foodborne Pathog Dis. 2010 Oct;7(10):1185-92
references.pmid: 20578916
references.citation: Antimicrob Agents Chemother. 2001 Mar;45(3):723-6
references.pmid: 11181350
references.citation: Microb Drug Resist. 2009 Dec;15(4):323-8
references.pmid: 19857140
references.citation: Int J Antimicrob Agents. 2008 Apr;31(4):360-3
references.pmid: 18162377
references.citation: Front Microbiol. 2011 Jul 26;2:158
references.pmid: 21845185
references.citation: Curr Opin Microbiol. 2003 Oct;6(5):439-45
references.pmid: 14572534
references.citation: J Food Prot. 2005 Dec;68(12):2693-7
references.pmid: 16355844
references.citation: Vet Res. 2011 Mar 02;42:44
references.pmid: 21366902
references.citation: Trends Microbiol. 2007 Jul;15(7):301-9
references.pmid: 17566739
references.citation: Nat Rev Microbiol. 2006 Aug;4(8):608-20
references.pmid: 16845431
references.citation: Anim Biotechnol. 2006;17(2):157-76
references.pmid: 17127527
references.citation: Int J Antimicrob Agents. 2000 May;14(4):295-301
references.pmid: 10794950
references.citation: J Antimicrob Chemother. 2004 Jun;53(6):906-17
references.pmid: 15102745
references.citation: Vet Microbiol. 2000 Mar 15;72(3-4):269-76
references.pmid: 10727836
references.citation: J Antimicrob Chemother. 2007 Dec;60(6):1243-50
references.pmid: 17913715
references.citation: Microb Drug Resist. 2008 Dec;14(4):265-72
references.pmid: 19090720
references.citation: Appl Environ Microbiol. 1994 Sep;60(9):3255-60
references.pmid: 7944364
references.citation: J Food Prot. 2007 Feb;70(2):292-9
references.pmid: 17340861
references.citation: Rev Peru Med Exp Salud Publica. 2011 Dec;28(4):648-56
references.pmid: 22241263