title: Molecular cloning and characterization of a novel SK3-type dehydrin gene from Stipa purpurea.
abstract: Stipa purpurea, an endemic forage of the grass family in the Tibetan Plateau, is highly resistant to drought. Dehydrins (DHNs) are stress proteins involved in plant protective reactions against environmental stress. In this study, the full-length DHN open reading frame (ORF) cloned from S. purpurea, named SpDHN1, was 816 nucleotides length and encoded a protein of 271 amino acids. Phylogenetic and sequence characterization analysis revealed that the DHN gene was an SK3-type DHNs. Subcellular localization analysis indicated that SpDHN1 was localized in the cytoplasm and the plasma membrane. SpDHN1 function analysis provided new evidence to support the antioxidation of SpDHN1 in plant responses drought stress. Ectopic expression of SpDHN1 in Arabidopsis thaliana plants showed more resistance to drought stress than the wild-type, indicating that SpDHN1 may be a potential candidate gene for genetic improvement of crops to improve stress tolerance.
authors.affiliation: Key Laboratory for Plant Diversity and Biogeography of Easst Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China; Plant Germplasm and Genomics Center, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China; Institute of Tibetan Plateau Research at Kunming, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Yunqiang
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Plant Germplasm and Genomics Center, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China; Institute of Tibetan Plateau Research at Kunming, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
authors.forename: Xudong
authors.initials: X
authors.lastname: Sun
authors.affiliation: Key Laboratory of Tibetan Environment Changes and Land Surface Processes, Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing 100085, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Shihai
authors.initials: S
authors.lastname: Yang
authors.affiliation: Plant Germplasm and Genomics Center, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China; Institute of Tibetan Plateau Research at Kunming, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Xiong
authors.initials: X
authors.lastname: Li
authors.affiliation: Plant Germplasm and Genomics Center, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China; Institute of Tibetan Plateau Research at Kunming, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China. Electronic address: yangyp@mail.kib.ac.cn.
authors.forename: Yongping
authors.initials: Y
authors.lastname: Yang
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics / Q000502:physiology; D001483:Base Sequence; D003001:Cloning, Molecular; D055864:Droughts; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D006109:Poaceae / Q000235:genetics* / Q000502:physiology; D013312:Stress, Physiological; D018609:Tibet
chemical_list: D010940:Plant Proteins; C070556:dehydrin proteins, plant
keywords: Antioxidation; Cloning; Dehydrin; Drought; Stipa purpurea