title: [Chemical structural features and anti-complementary activity of polysaccharide HPS1-D from Hedysarum polybotrys].
abstract: HPS1-D, an active polysaccharide,was isolated and purified from Hedysarum polybotrys. HPS1-D was obtained after treated with Savage method and H2O2, and purified with DEAE-cellulose 52 and Sephadex G-100 gel filtration chromatography. Then physicochemical property analysis, GC, methylation, partial acid hydrolysis, and NMR method were used to study chemical structural of HPS1-D. The conformation was primarily analyzed with GPC-MALLS method and Congo red reaction. The anti-complementary activity of HPS1-D was evaluated with the hemolysis assay. HPS1-D was a heteropolysaccharide and consisted of D-glucose, L-arabinose, (7.2:1.3). HPS1-D proved to be a neutral sugar, with 1, 4-and 1, 4, 6-alpha-D-glucopyranosyl residues in backbone ,and 1, 5-and 1, 3, 5-alpha-L-arabinofuranosyl residues in branches. HPS1-D has a random coil state conformation with monodisperse mass distribution in 0.9% NaCl solution. And HPS1-D had triple-helix conformation in concentrate of NaOH solution. Anti-complementary activity of HPS1-D was closed to its positive control heparin.
authors.forename: Tao
authors.initials: T
authors.lastname: Yang
authors.forename: Long
authors.initials: L
authors.lastname: Guo
authors.forename: Can
authors.initials: C
authors.lastname: Li
authors.forename: Ying-Lai
authors.initials: YL
authors.lastname: Yang
authors.forename: Shi-Lan
authors.initials: SL
authors.lastname: Feng
mesh_terms: D000818:Animals; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000494:pharmacology*; D006461:Hemolysis / Q000187:drug effects; D051379:Mice; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; D011134:Polysaccharides