title: Determination of a dynamic feeding strategy for recombinant Pichia pastoris strains.
abstract: The knowledge of certain strain specific parameters of recombinant P. pastoris strains is required to be able to set up a feeding regime for fed-batch cultivations. To date, these parameters are commonly determined either by time-consuming and labor-intensive continuous cultivations or by several, consecutive fed-batch cultivations. Here, we describe a fast method based on batch experiments with methanol pulses to extract certain strain characteristic parameters, which are required to set up a dynamic feeding strategy for P. pastoris strains based on specific substrate uptake rate (q(s)). We further describe in detail the course of actions which have to be taken to obtain the desired dynamics during feeding.
authors.affiliation: Research Area Biochemical Engineering, Institute of Chemical Engineering, Vienna University of Technology, Gumpendorfer Strasse 1a, A-1060, Vienna, Austria.
authors.forename: Oliver
authors.initials: O
authors.lastname: Spadiut
authors.forename: Christian
authors.initials: C
authors.lastname: Dietzsch
authors.forename: Christoph
authors.initials: C
authors.lastname: Herwig
mesh_terms: D061249:Batch Cell Culture Techniques / Q000379:methods*; D019149:Bioreactors / Q000382:microbiology; D000432:Methanol / Q000494:pharmacology; D010843:Pichia / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development*; D011994:Recombinant Proteins / Q000096:biosynthesis*; D013045:Species Specificity; D013997:Time Factors
chemical_list: D011994:Recombinant Proteins; D000432:Methanol
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