title: Transamidase subunit GAA1/GPAA1 is a M28 family metallo-peptide-synthetase that catalyzes the peptide bond formation between the substrate protein's omega-site and the GPI lipid anchor's phosphoethanolamine.
abstract: The transamidase subunit GAA1/GPAA1 is predicted to be the enzyme that catalyzes the attachment of the glycosylphosphatidyl (GPI) lipid anchor to the carbonyl intermediate of the substrate protein at the ω-site. Its ~300-amino acid residue lumenal domain is a M28 family metallo-peptide-synthetase with an α/β hydrolase fold, including a central 8-strand β-sheet and a single metal (most likely zinc) ion coordinated by 3 conserved polar residues. Phosphoethanolamine is used as an adaptor to make the non-peptide GPI lipid anchor look chemically similar to the N terminus of a peptide.
authors.affiliation: Bioinformatics Institute (BII); A*STAR; Singapore, Republic of Singapore.
authors.forename: Birgit
authors.initials: B
authors.lastname: Eisenhaber
authors.affiliation: Department of Physical Chemistry; University of Vienna; Wien/Vienna, Republic of Austria.
authors.forename: Stephan
authors.initials: S
authors.lastname: Eisenhaber
authors.affiliation: Bioinformatics Institute (BII); A*STAR; Singapore, Republic of Singapore.
authors.forename: Toh Yew
authors.initials: TY
authors.lastname: Kwang
authors.affiliation: Bioinformatics Institute (BII); A*STAR; Singapore, Republic of Singapore; Nanyang Technological University; School of Biological Sciences; Singapore, Republic of Singapore.
authors.forename: Gerhard
authors.initials: G
authors.lastname: Grüber
authors.affiliation: Bioinformatics Institute (BII); A*STAR; Singapore, Republic of Singapore; Department of Biological Sciences (DBS); National University of Singapore (NUS); Singapore, Republic of Singapore; School of Computer Engineering (SCE); Nanyang Technological University (NTU); Singapore, Republic of Singapore.
authors.forename: Frank
authors.initials: F
authors.lastname: Eisenhaber
mesh_terms: D019881:Aminoacyltransferases / Q000737:chemistry / Q000378:metabolism*; D004983:Ethanolamines / Q000378:metabolism*; D017261:Glycosylphosphatidylinositols / Q000378:metabolism*; D006801:Humans; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000378:metabolism*; D010453:Peptide Synthases / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry*; D050050:Protein Carbonylation; D021122:Protein Subunits / Q000737:chemistry / Q000378:metabolism*
chemical_list: D004983:Ethanolamines; C093316:GPAA1 protein, human; D017261:Glycosylphosphatidylinositols; D008562:Membrane Glycoproteins; D010455:Peptides; D021122:Protein Subunits; C005448:phosphorylethanolamine; D019881:Aminoacyltransferases; C023175:transamidases; D010453:Peptide Synthases
keywords: GAA1; GPAA1; GPI lipid anchor; GPI transamidase; M28 peptidase; metallo-peptide-synthetase; protein sequence; sequence analysis
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