title: Immune regulation and anti-inflammatory effects of isogarcinol extracted from Garcinia mangostana L. against collagen-induced arthritis.
abstract: Isogarcinol is a natural compound that we extracted from Garcinia mangostana L., and we were the first to report that it is a new immunosuppressant. In the present study, we investigated the immune regulation and anti-inflammatory effects of isogarcinol on collagen-induced arthritis (CIA) and explored its potential mechanism in the treatment of rheumatoid arthritis. The oral administration of isogarcinol significantly reduced clinical scores, alleviated cartilage and bone erosion, and reduced the levels of serum inflammatory cytokines in CIA mice. Isogarcinol inhibited xylene-induced mouse ear edema in vivo. In vitro, isogarcinol decreased iNOS and COX-2 mRNA expression and NO content by inhibiting NF-κB expression. Furthermore, isogarcinol decreased the activity of NFAT and inhibited IL-2 expression. The mechanism of action of isogarcinol is associated with down-regulation of both autoimmune and inflammatory reactions.
authors.affiliation: Department of Biochemistry and Molecular Biology, Beijing Normal University , Gene Engineering and Biotechnology Beijing Key Laboratory, Beijing 100875, People's Republic of China.
authors.forename: Yanxia
authors.initials: Y
authors.lastname: Fu
authors.forename: Hailing
authors.initials: H
authors.lastname: Zhou
authors.forename: Mengqi
authors.initials: M
authors.lastname: Wang
authors.forename: Juren
authors.initials: J
authors.lastname: Cen
authors.forename: Qun
authors.initials: Q
authors.lastname: Wei
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage*; D001169:Arthritis, Experimental / Q000188:drug therapy* / Q000235:genetics / Q000276:immunology; D004195:Disease Models, Animal; D029762:Garcinia mangostana / Q000737:chemistry*; D006801:Humans; D007155:Immunologic Factors / Q000235:genetics / Q000276:immunology; D007376:Interleukin-2 / Q000235:genetics / Q000276:immunology; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D008813:Mice, Inbred ICR; D016328:NF-kappa B / Q000235:genetics / Q000276:immunology; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000276:immunology; D010936:Plant Extracts / Q000008:administration & dosage*
chemical_list: D000893:Anti-Inflammatory Agents; D007155:Immunologic Factors; D007376:Interleukin-2; D016328:NF-kappa B; D010936:Plant Extracts; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse
keywords: Garcinia mangostana Linn.; collagen-induced arthritis; immunity; inflammation; isogarcinol