title: Interaction between pirenoxine and bovine serum albumin in aqueous solution.
abstract: This work concerns the interaction of prenoxine sodium (PRX) and bovine serum albumin (BSA), which was conducted by spectroscopic means: fluorescence spectra, ultraviolet-visible spectra (UV-vis) and circular dichroism spectra (CD spectra) in physiological conditions. The results revealed the PRX can quench the fluorescence of BSA remarkably in aqueous solution. The quench mechanism has been obtained after corrected the fluorescence intensities for inner filter effects. The binding constants (Ka) were calculated according to the relevant fluorescence data at different temperatures. Moreover, from a series of analyses, we have obtained the binding sites, the binding distance and binding force. The effect of PRX on the conformation of BSA has been analyzed using synchronous fluorescence under experimental conditions. In addition, the CD spectra proved that the secondary structure of BSA changed in the presence of PRX in aqueous solution.
authors.affiliation: Key Laboratory of Theoretical Chemistry and Molecular Simulation of Ministry of Education, Hunan Province College Key Laboratory of QSAR/QSPR, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Xiangtan 411201, PR China.
authors.forename: Zhixi
authors.initials: Z
authors.lastname: Liao
authors.affiliation: Key Laboratory of Theoretical Chemistry and Molecular Simulation of Ministry of Education, Hunan Province College Key Laboratory of QSAR/QSPR, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Xiangtan 411201, PR China; State Key Laboratory of Physical Chemistry of Solid Surfaces, Xiamen University, Xiamen 361005, PR China; Key Laboratory of Computational Physical Sciences (Fudan University), Ministry of Education, Shanghai 200433, PR China. Electronic address: yu_xianyong@163.com.
authors.forename: Xianyong
authors.initials: X
authors.lastname: Yu
authors.affiliation: Key Laboratory of Theoretical Chemistry and Molecular Simulation of Ministry of Education, Hunan Province College Key Laboratory of QSAR/QSPR, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Xiangtan 411201, PR China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Yao
authors.affiliation: Key Laboratory of Theoretical Chemistry and Molecular Simulation of Ministry of Education, Hunan Province College Key Laboratory of QSAR/QSPR, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Xiangtan 411201, PR China. Electronic address: pgyi@hnust.cn.
authors.forename: Pinggui
authors.initials: P
authors.lastname: Yi
mesh_terms: D000818:Animals; D001665:Binding Sites; D002386:Cataract / Q000188:drug therapy; D002417:Cattle; D002942:Circular Dichroism; D006801:Humans; D010078:Oxazines / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D011487:Protein Conformation / Q000187:drug effects; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism*; D012996:Solutions; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D014867:Water / Q000737:chemistry
chemical_list: D010078:Oxazines; D012996:Solutions; D014867:Water; D012710:Serum Albumin, Bovine; C004580:pirenoxine
keywords: Bovine serum albumin; Circular dichroism spectra; Interaction; Prenoxine; Spectroscopic means