title: The effect of mycotoxin deoxynivalenol on haematological and biochemical indicators and histopathological changes in rainbow trout (Oncorhynchus mykiss).
abstract: Deoxynivalenol (DON), produced by the Fusarium genus, is a major contaminant of cereal grains used in the production of fish feed. The effect of mycotoxin deoxynivalenol on rainbow trout (Oncorhynchus mykiss) was studied using a commercial feed with the addition of DON in a dose of 2 mg/kg feed. The fish (n=40) were exposed to the mycotoxin for 23 days. The trout were divided into two groups, control and experimental groups. Control groups were fed a commercial feed naturally contaminated with a low concentration of DON (225  μg/kg feed); experimental groups were fed a commercial feed with the addition of DON (1964  μg/kg feed). Plasma biochemical and haematological indices, biometric parameters, and histopathological changes were assessed at the end of the experiment. The experimental groups showed significantly lower values in MCH (P<0.05). In biochemical indices, after 23-day exposure, a significant decrease in glucose, cholesterol (P<0.05), and ammonia (P<0.01) was recorded in the experimental group compared to the control group. Our assessment showed no significant changes in biometric parameters. The histopathological examination revealed disorders in the caudal kidney of the exposed fish. The obtained data show the sensitivity of rainbow trout (O. mykiss) to deoxynivalenol.
authors.affiliation: Department of Veterinary Public Health and Welfare, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho tr. 1/3, 612 42 Brno, Czech Republic.
authors.forename: Iveta
authors.identifier: 0000-0001-8610-7033
authors.initials: I
authors.lastname: Matejova
authors.affiliation: Department of Veterinary Public Health and Welfare, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho tr. 1/3, 612 42 Brno, Czech Republic.
authors.forename: Helena
authors.initials: H
authors.lastname: Modra
authors.affiliation: Department of Veterinary Public Health and Welfare, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho tr. 1/3, 612 42 Brno, Czech Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Blahova
authors.affiliation: Department of Pharmaceutics and Biopharmacy, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho tr. 1/3, 612 42 Brno, Czech Republic.
authors.forename: Ales
authors.initials: A
authors.lastname: Franc
authors.affiliation: Department of Pathological Morphology and Parasitology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho tr. 1/3, 612 42 Brno, Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Fictum
authors.affiliation: Department of Veterinary Public Health and Welfare, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho tr. 1/3, 612 42 Brno, Czech Republic.
authors.forename: Marie
authors.initials: M
authors.lastname: Sevcikova
authors.affiliation: Department of Veterinary Public Health and Welfare, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho tr. 1/3, 612 42 Brno, Czech Republic.
authors.forename: Zdenka
authors.initials: Z
authors.lastname: Svobodova
mesh_terms: D000821:Animal Feed / Q000633:toxicity*; D000818:Animals; D001798:Blood Proteins / Q000032:analysis*; D005506:Food Contamination; D009183:Mycotoxins / Q000633:toxicity*; D017686:Oncorhynchus mykiss / Q000033:anatomy & histology* / Q000097:blood* / Q000254:growth & development; D009929:Organ Size / Q000187:drug effects; D014255:Trichothecenes / Q000633:toxicity*
chemical_list: D001798:Blood Proteins; D009183:Mycotoxins; D014255:Trichothecenes; C007262:deoxynivalenol
references.citation: Toxicol Lett. 2004 Oct 10;153(1):61-73
references.pmid: 15342082
references.citation: J Agric Food Chem. 2008 Feb 13;56(3):1029-34
references.pmid: 18197623
references.citation: Toxins (Basel). 2013 Jan 16;5(1):184-92
references.pmid: 23325300
references.citation: Food Chem Toxicol. 2012 Dec;50(12):4441-8
references.pmid: 22975143
references.citation: Toxins (Basel). 2012 Nov 06;4(11):1157-80
references.pmid: 23202310
references.citation: Exp Toxicol Pathol. 2012 Nov;64(7-8):681-7
references.pmid: 21282048
references.citation: Environ Toxicol Pharmacol. 2013 Jul;36(1):1-8
references.pmid: 23538322