title: Plant autophagy is responsible for peroxisomal transition and plays an important role in the maintenance of peroxisomal quality.
abstract: In photosynthetic cells, a large amount of hydrogen peroxide is produced in peroxisomes through photorespiration, which is a metabolic pathway related to photosynthesis. Hydrogen peroxide, a reactive oxygen species, oxidizes peroxisomal proteins and membrane lipids, resulting in a decrease in peroxisomal quality. We demonstrate that the autophagic system is responsible for the elimination of oxidized peroxisomes in plant. We isolated Arabidopsis mutants that accumulated oxidized peroxisomes, which formed large aggregates. We revealed that these mutants were defective in autophagy-related (ATG) genes and that the aggregated peroxisomes were selectively targeted by the autophagic machinery. These findings suggest that autophagy plays an important role in the quality control of peroxisomes by the selective degradation of oxidized peroxisomes. In addition, the results suggest that autophagy is also responsible for the functional transition of glyoxysomes to leaf peroxisomes.
authors.affiliation: Department of Cell Biology; National Institute for Basic Biology; Okazaki, Japan; Department of Basic Biology; School of Life Science; Graduate University for Advanced Studies; Okazaki, Japan.
authors.forename: Michitaro
authors.initials: M
authors.lastname: Shibata
authors.affiliation: Department of Applied Biological Chemistry; Niigata University; Niigata, Japan.
authors.forename: Kazusato
authors.initials: K
authors.lastname: Oikawa
authors.affiliation: INRA; UMR1318; Institut Jean-Pierre Bourgin; Versailles, France; AgroParisTech; Institut Jean-Pierre Bourgin; Versailles, France.
authors.forename: Kohki
authors.initials: K
authors.lastname: Yoshimoto
authors.affiliation: Department of Cell Biology; National Institute for Basic Biology; Okazaki, Japan.
authors.forename: Shino
authors.initials: S
authors.lastname: Goto-Yamada
authors.affiliation: Department of Cell Biology; National Institute for Basic Biology; Okazaki, Japan; Department of Basic Biology; School of Life Science; Graduate University for Advanced Studies; Okazaki, Japan.
authors.forename: Shoji
authors.initials: S
authors.lastname: Mano
authors.affiliation: Department of Cell Biology; National Institute for Basic Biology; Okazaki, Japan; Department of Basic Biology; School of Life Science; Graduate University for Advanced Studies; Okazaki, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Yamada
authors.affiliation: Department of Cell Biology; National Institute for Basic Biology; Okazaki, Japan.
authors.forename: Maki
authors.initials: M
authors.lastname: Kondo
authors.affiliation: Department of Bioscience; Nagahama Institute of Bio-Science and Technology; Nagahama, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Hayashi
authors.affiliation: Institute of Plant Science and Resources; Okayama University; Okayama, Japan.
authors.forename: Wataru
authors.initials: W
authors.lastname: Sakamoto
authors.affiliation: Frontier Research Center; Tokyo Institute of Technology; Yokohama, Japan.
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Ohsumi
authors.affiliation: Department of Cell Biology; National Institute for Basic Biology; Okazaki, Japan; Department of Basic Biology; School of Life Science; Graduate University for Advanced Studies; Okazaki, Japan.
authors.forename: Mikio
authors.initials: M
authors.lastname: Nishimura
mesh_terms: D017360:Arabidopsis / Q000502:physiology*; D001343:Autophagy / Q000502:physiology*; D006861:Hydrogen Peroxide / Q000378:metabolism; D053858:Metabolic Networks and Pathways; D010084:Oxidation-Reduction; D020675:Peroxisomes / Q000378:metabolism*; D018515:Plant Leaves / Q000378:metabolism; D030821:Plants, Genetically Modified; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide
keywords: Arabidopsis thaliana; glyoxysome leaf peroxisome; hydrogen peroxide; peroxisome; peroxisome transition; pexophagy