title: Extraction, characterization and antioxidant activities of polysaccharides from E. corneum gigeriae galli.
abstract: In the present study, optimization of enzyme-assisted extraction, characterization and antioxidant activities in vitro of polysaccharides from Endothelium corneum gigeriae galli (PEGG) were investigated. It was found that the optimum extraction conditions were determined as follows: extraction temperature 87.0°C, extraction time 177.0 min, enzyme concentration 1.65%, enzymatic hydrolysis time 141.0 min, liquid-to-solid ratio 20, enzymatic hydrolysis temperature 55°C and enzymatic hydrolysis pH 3.6. Under these conditions, the experimental yield of polysaccharides was 5.08%. In addition, PEGG had a relatively high sulfate radical content. PEGG was composed of rhamnose, fucose, mannose, glucose and galactose, with molar percentages of 13.1, 4.5, 72.8 and 9.6%, respectively. The average molecular weight was 83 kDa. And there were infrared characteristic absorption peaks of polysaccharides in the FT-IR spectroscopy of PEGG. For antioxidant activities in vitro, PEGG showed possessed strong hydroxyl radical scavenging, Fe(2+) chelating and lipid peroxidation inhibitory activities.
authors.affiliation: School of Life Science and Chemical Engineering, Huaiyin Institute of Technology, Huaian 223001, Jiangsu, PR China; Jiangsu Provincial Engineering Laboratory for Biomass Conversion and Process Integration, Huaiyin Institute of Technology, Huaian 223003, Jiangsu, PR China.
authors.forename: Qingping
authors.initials: Q
authors.lastname: Xiong
authors.affiliation: Affiliated Huaian Hospital of Xuzhou Medical College, Huaian 223002, Jiangsu, PR China.
authors.forename: Xia
authors.initials: X
authors.lastname: Li
authors.affiliation: School of Basic Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, Guangdong, PR China.
authors.forename: Ruizhen
authors.initials: R
authors.lastname: Zhou
authors.affiliation: Affiliated Huaian Hospital of Xuzhou Medical College, Huaian 223002, Jiangsu, PR China.
authors.forename: Hairong
authors.initials: H
authors.lastname: Hao
authors.affiliation: School of Life Science and Chemical Engineering, Huaiyin Institute of Technology, Huaian 223001, Jiangsu, PR China.
authors.forename: Songlin
authors.initials: S
authors.lastname: Li
authors.affiliation: School of Life Science and Chemical Engineering, Huaiyin Institute of Technology, Huaian 223001, Jiangsu, PR China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Jing
authors.affiliation: School of Life Science and Chemical Engineering, Huaiyin Institute of Technology, Huaian 223001, Jiangsu, PR China; Jiangsu Provincial Engineering Laboratory for Biomass Conversion and Process Integration, Huaiyin Institute of Technology, Huaian 223003, Jiangsu, PR China.
authors.forename: Chun
authors.initials: C
authors.lastname: Zhu
authors.affiliation: School of Life Science and Chemical Engineering, Huaiyin Institute of Technology, Huaian 223001, Jiangsu, PR China.
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: School of Life Science and Chemical Engineering, Huaiyin Institute of Technology, Huaian 223001, Jiangsu, PR China. Electronic address: shiyingying82012@163.com.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Shi
mesh_terms: D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification*; D008419:Materia Medica / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification*
chemical_list: D000975:Antioxidants; D008419:Materia Medica; D011134:Polysaccharides
keywords: Antioxidant activity; Characterization; E. corneum gigeriae galli; Enzyme-assisted extraction; Polysaccharide