title: The role of Hypoxia-inducible factor-1 α , glucose transporter-1, (GLUT-1) and carbon anhydrase IX in endometrial cancer patients.
abstract: Hypoxia-inducible factor-1α (HIF-1α), glucose transporter-1 (GLUT-1), and carbon anhydrase IX (CAIX) are important molecules that allow adaptation to hypoxic environments. The aim of our study was to investigate the correlation between HIF-1α, GLUT-1, and CAIX protein level with the clinicopathological features of endometrial cancer patients. Materials and Methods. 92 endometrial cancer patients, aged 37-84, were enrolled to our study. In all patients clinical stage, histologic grade, myometrial invasion, lymph node, and distant metastases were determined. Moreover, the survival time was assessed. Immunohistochemical analyses were performed on archive formalin fixed paraffin embedded tissue sections. Results. High significant differences (P = 0.0115) were reported between HIF-1α expression and the histologic subtype of cancer. Higher HIF-1α expression was associated with the higher risk of recurrence (P = 0.0434). The results of GLUT-1 and CAIX expression did not reveal any significant differences between the proteins expression in the primary tumor and the clinicopathological features. Conclusion. The important role of HIF-1α in the group of patients with the high risk of recurrence and the negative histologic subtype of the tumor suggest that the expression of this factor might be useful in the panel of accessory pathomorphological tests and could be helpful in establishing more accurate prognosis in endometrial cancer patients.
authors.affiliation: Department of Obstetrics and Gynecology, The Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University of Torun, Ulica Ujejskiego 75, 85-168 Bydgoszcz, Poland.
authors.forename: Pawel
authors.initials: P
authors.lastname: Sadlecki
authors.affiliation: Department of Clinical Pathology, The Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University of Torun, 87-100 Torun, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Bodnar
authors.affiliation: Department of Obstetrics and Gynecology, The Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University of Torun, Ulica Ujejskiego 75, 85-168 Bydgoszcz, Poland.
authors.forename: Marek
authors.initials: M
authors.lastname: Grabiec
authors.affiliation: Department of Clinical Pathology, The Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University of Torun, 87-100 Torun, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Marszalek
authors.affiliation: Department of Obstetrics and Gynecology, The Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University of Torun, Ulica Ujejskiego 75, 85-168 Bydgoszcz, Poland.
authors.forename: Pawel
authors.identifier: 0000-0002-7748-343X
authors.initials: P
authors.lastname: Walentowicz
authors.affiliation: Department of Gastroenterology, Angiology and Internal Diseases, The Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University of Torun, 87-100 Torun, Poland.
authors.forename: Alina
authors.initials: A
authors.lastname: Sokup
authors.affiliation: Department of Obstetrics and Gynecology, The Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University of Torun, Ulica Ujejskiego 75, 85-168 Bydgoszcz, Poland.
authors.forename: Jolanta
authors.identifier: 0000-0002-3278-1708
authors.initials: J
authors.lastname: Zegarska
authors.affiliation: Department of Obstetrics and Gynecology, The Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University of Torun, Ulica Ujejskiego 75, 85-168 Bydgoszcz, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Walentowicz-Sadlecka
mesh_terms: D000328:Adult; D000368:Aged; D000951:Antigens, Neoplasm / Q000378:metabolism*; D000071231:Carbonic Anhydrase IX; D002256:Carbonic Anhydrases / Q000378:metabolism*; D018572:Disease-Free Survival; D016889:Endometrial Neoplasms / Q000378:metabolism / Q000401:mortality / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D051272:Glucose Transporter Type 1 / Q000378:metabolism*; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism*; D007150:Immunohistochemistry; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000378:metabolism*; D012189:Retrospective Studies; D015996:Survival Rate
chemical_list: D000951:Antigens, Neoplasm; D051272:Glucose Transporter Type 1; C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D009363:Neoplasm Proteins; C495677:SLC2A1 protein, human; C089226:CA9 protein, human; D000071231:Carbonic Anhydrase IX; D002256:Carbonic Anhydrases
references.citation: Cell Cycle. 2004 Feb;3(2):164-7
references.pmid: 14712082
references.citation: J Clin Oncol. 2011 Aug 20;29(24):3278-85
references.pmid: 21788564
references.citation: Proc Natl Acad Sci U S A. 1998 Nov 10;95(23):13513-8
references.pmid: 9811831
references.citation: Virology. 2009 May 10;387(2):442-8
references.pmid: 19321184
references.citation: Horm Metab Res. 2012 Jun;44(6):436-41
references.pmid: 22351475
references.citation: Cancer Biol Ther. 2009 Feb;8(4):322-30
references.pmid: 19305150
references.citation: J Biol Chem. 2009 Nov 13;284(46):32066-74
references.pmid: 19762474
references.citation: J Appl Physiol (1985). 2006 Sep;101(3):853-65
references.pmid: 16728520
references.citation: Cancer. 2002 Sep 1;95(5):1055-63
references.pmid: 12209691
references.citation: Histol Histopathol. 2012 Oct;27(10):1247-53
references.pmid: 22936443
references.citation: Endocrinology. 2006 Feb;147(2):744-53
references.pmid: 16282352
references.citation: BMC Cancer. 2010 Jun 19;10:307
references.pmid: 20565904
references.citation: Mod Pathol. 2007 Jan;20(1):35-43
references.pmid: 17099695
references.citation: Cell Oncol. 2007;29(3):219-27
references.pmid: 17452774
references.citation: Cancer Res. 1999 Nov 15;59(22):5830-5
references.pmid: 10582706
references.citation: Int J Biochem Mol Biol. 2012;3(2):165-78
references.pmid: 22773957
references.citation: Cancer. 2004 Jun 1;100(11):2376-86
references.pmid: 15160341
references.citation: Cancer Lett. 2000 Jun 30;154(2):175-82
references.pmid: 10806305
references.citation: APMIS. 2012 Feb;120(2):117-29
references.pmid: 22229267
references.citation: Circ Res. 2003 Nov 28;93(11):1074-81
references.pmid: 14576200
references.citation: Oncology. 2006;71(1-2):95-101
references.pmid: 17341889
references.citation: Brain Res. 2009 Dec 22;1304:149-54
references.pmid: 19782666
references.citation: J Endocrinol. 2004 Oct;183(1):145-54
references.pmid: 15525582
references.citation: J Clin Pathol. 2008 Feb;61(2):217-20
references.pmid: 17513511
references.citation: Semin Cell Dev Biol. 2012 Jun;23(4):389-94
references.pmid: 22525300
references.citation: Eur J Gynaecol Oncol. 2010;31(2):160-4
references.pmid: 20527231
references.citation: Obstet Gynecol Int. 2010;2010:580971
references.pmid: 20169098
references.citation: Clin Cancer Res. 2001 Oct;7(10):3097-105
references.pmid: 11595701
references.citation: Carcinogenesis. 2008 Oct;29(10):1853-61
references.pmid: 18339685
references.citation: Oncol Rep. 2009 Mar;21(3):707-12
references.pmid: 19212630
references.citation: CA Cancer J Clin. 2010 Sep-Oct;60(5):277-300
references.pmid: 20610543
references.citation: J Clin Endocrinol Metab. 2011 Aug;96(8):2475-83
references.pmid: 21677035
references.citation: Expert Rev Mol Diagn. 2009 Apr;9(3):231-41
references.pmid: 19379082
references.citation: J Cell Physiol. 2008 Jan;214(1):47-55
references.pmid: 17559070
references.citation: Cancer Detect Prev. 2007;31(2):102-9
references.pmid: 17418979
references.citation: Trends Pharmacol Sci. 2006 Nov;27(11):566-73
references.pmid: 16996620
references.citation: Mayo Clin Proc. 2008 Jan;83(1):97-112
references.pmid: 18174012
references.citation: PLoS One. 2011 May 12;6(5):e19209
references.pmid: 21589857
references.citation: J Biol Chem. 2001 Mar 23;276(12):9519-25
references.pmid: 11120745
references.citation: Pathologe. 1987 May;8(3):138-40
references.pmid: 3303008
references.citation: Lancet. 2005 Aug 6-12;366(9484):491-505
references.pmid: 16084259
references.citation: Science. 1956 Feb 24;123(3191):309-14
references.pmid: 13298683