title: Interaction of a partially disordered antisigma factor with its partner, the signaling domain of the TonB-dependent transporter HasR.
abstract: Bacteria use diverse signaling pathways to control gene expression in response to external stimuli. In Gram-negative bacteria, the binding of a nutrient is sensed by an outer membrane transporter. This signal is then transmitted to an antisigma factor and subsequently to the cytoplasm where an ECF sigma factor induces expression of genes related to the acquisition of this nutrient. The molecular interactions involved in this transmembrane signaling are poorly understood and structural data on this family of antisigma factor are rare. Here, we present the first structural study of the periplasmic domain of an antisigma factor and its interaction with the transporter. The study concerns the signaling in the heme acquisition system (Has) of Serratia marcescens. Our data support unprecedented partially disordered periplasmic domain of an anti-sigma factor HasS in contact with a membrane-mimicking environment. We solved the 3D structure of the signaling domain of HasR transporter and identified the residues at the HasS-HasR interface. Their conservation in several bacteria suggests wider significance of the proposed model for the understanding of bacterial transmembrane signaling.
authors.affiliation: Institut Pasteur, Unité de RMN des Biomolécules, Département de Biologie Structurale et Chimie, Paris, France; CNRS, UMR 3528, Paris, France; Université Pierre et Marie Curie, Cellule Pasteur UPMC, Paris, France.
authors.forename: Idir
authors.initials: I
authors.lastname: Malki
authors.affiliation: Institut Pasteur, Unité de RMN des Biomolécules, Département de Biologie Structurale et Chimie, Paris, France; CNRS, UMR 3528, Paris, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Simenel
authors.affiliation: Institut Pasteur, Unité de RMN des Biomolécules, Département de Biologie Structurale et Chimie, Paris, France; CNRS, UMR 3528, Paris, France.
authors.forename: Halina
authors.initials: H
authors.lastname: Wojtowicz
authors.affiliation: Institut Pasteur, Unité de RMN des Biomolécules, Département de Biologie Structurale et Chimie, Paris, France; CNRS, UMR 3528, Paris, France.
authors.forename: Gisele Cardoso
authors.initials: GC
authors.lastname: de Amorim
authors.affiliation: Institut Pasteur, Unité de RMN des Biomolécules, Département de Biologie Structurale et Chimie, Paris, France; CNRS, UMR 3528, Paris, France.
authors.forename: Ada
authors.initials: A
authors.lastname: Prochnicka-Chalufour
authors.affiliation: Institut Pasteur, Plate-forme de Biophysique des Macromolécules et de leurs Interactions, Département de Biologie Structurale et Chimie, Paris, France.
authors.forename: Sylviane
authors.initials: S
authors.lastname: Hoos
authors.affiliation: Institut Pasteur, Plate-forme de Biophysique des Macromolécules et de leurs Interactions, Département de Biologie Structurale et Chimie, Paris, France.
authors.forename: Bertrand
authors.initials: B
authors.lastname: Raynal
authors.affiliation: Institut Pasteur, Plate-forme de Biophysique des Macromolécules et de leurs Interactions, Département de Biologie Structurale et Chimie, Paris, France.
authors.forename: Patrick
authors.initials: P
authors.lastname: England
authors.affiliation: Institut Pasteur, Unité de RMN des Biomolécules, Département de Biologie Structurale et Chimie, Paris, France; CNRS, UMR 3528, Paris, France.
authors.forename: Alain
authors.initials: A
authors.lastname: Chaffotte
authors.affiliation: Institut Pasteur, Unité de RMN des Biomolécules, Département de Biologie Structurale et Chimie, Paris, France; CNRS, UMR 3528, Paris, France.
authors.forename: Muriel
authors.initials: M
authors.lastname: Delepierre
authors.affiliation: Institut de Biologie Physico-Chimique, CNRS Université Paris-Diderot UMR 7099, Paris, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Delepelaire
authors.affiliation: Institut Pasteur, Unité de RMN des Biomolécules, Département de Biologie Structurale et Chimie, Paris, France; CNRS, UMR 3528, Paris, France.
authors.forename: Nadia
authors.initials: N
authors.lastname: Izadi-Pruneyre
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000378:metabolism*; D019897:Periplasm / Q000378:metabolism; D011485:Protein Binding; D012706:Serratia marcescens / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology*
chemical_list: D001426:Bacterial Proteins; D026901:Membrane Transport Proteins
references.citation: Infect Immun. 2004 Feb;72(2):896-907
references.pmid: 14742534
references.citation: Mol Microbiol. 2004 Aug;53(4):1267-77
references.pmid: 15306027
references.citation: J Mol Biol. 2012 Nov 9;423(5):818-30
references.pmid: 22982293
references.citation: J Mol Biol. 2007 Apr 27;368(2):398-406
references.pmid: 17349657
references.citation: PLoS One. 2013;8(3):e58964
references.pmid: 23527057
references.citation: Methods. 2013 Mar;59(3):372-81
references.pmid: 23267861
references.citation: PLoS Pathog. 2009 Sep;5(9):e1000572
references.pmid: 19730690
references.citation: J Am Chem Soc. 2009 Dec 16;131(49):17972-9
references.pmid: 19995078
references.citation: Biochim Biophys Acta. 2008 Sep;1778(9):1781-804
references.pmid: 17916327
references.citation: Methods. 2011 May;54(1):56-66
references.pmid: 21112401
references.citation: Curr Opin Microbiol. 2003 Apr;6(2):173-80
references.pmid: 12732308
references.citation: Biochim Biophys Acta. 2013 Apr;1834(4):808-16
references.pmid: 23352839
references.citation: J Mol Biol. 2009 Nov 13;393(5):1129-42
references.pmid: 19747487
references.citation: Nature. 1996 May 23;381(6580):272
references.pmid: 8692262
references.citation: Structure. 2007 Nov;15(11):1383-91
references.pmid: 17997964
references.citation: Proteins. 2005 Jun 1;59(4):687-96
references.pmid: 15815974
references.citation: Nucleic Acids Res. 2010 Jul;38(Web Server issue):W545-9
references.pmid: 20457744
references.citation: Mol Microbiol. 2003 Jun;48(6):1467-80
references.pmid: 12791131
references.citation: Biometals. 2009 Aug;22(4):671-8
references.pmid: 19153809
references.citation: Methods Enzymol. 2001;339:71-90
references.pmid: 11462826
references.citation: Biochim Biophys Acta. 2008 Sep;1778(9):1930-45
references.pmid: 17673165
references.citation: Mol Microbiol. 2005 Dec;58(5):1226-37
references.pmid: 16313612
references.citation: J Biomol NMR. 2000 Jan;16(1):23-8
references.pmid: 10718609
references.citation: Proc Natl Acad Sci U S A. 2007 Jan 9;104(2):513-8
references.pmid: 17197416
references.citation: Nat Rev Mol Cell Biol. 2005 Mar;6(3):197-208
references.pmid: 15738986
references.citation: J Bacteriol. 2006 Sep;188(17):6440-2
references.pmid: 16923915
references.citation: Methods. 2004 Sep;34(1):121-32
references.pmid: 15283921
references.citation: Acta Crystallogr D Biol Crystallogr. 1998 Sep 1;54(Pt 5):905-21
references.pmid: 9757107
references.citation: Methods. 2007 Apr;41(4):398-408
references.pmid: 17367712
references.citation: Mol Microbiol. 2012 Aug;85(4):618-31
references.pmid: 22715905
references.citation: Biochemistry. 1981 Jan 6;20(1):33-7
references.pmid: 7470476
references.citation: J Mol Recognit. 2010 Mar-Apr;23(2):105-16
references.pmid: 19585546
references.citation: J Bacteriol. 1997 Jun;179(11):3572-9
references.pmid: 9171402
references.citation: Structure. 2006 Apr;14(4):683-93
references.pmid: 16615910
references.citation: Biophys J. 2006 Jun 15;90(12):4651-61
references.pmid: 16565040
references.citation: J Biomol NMR. 1995 Nov;6(3):277-93
references.pmid: 8520220
references.citation: J Am Chem Soc. 2012 Jun 20;134(24):10080-8
references.pmid: 22621369
references.citation: Curr Opin Struct Biol. 2006 Feb;16(1):109-17
references.pmid: 16427776
references.citation: J Bacteriol. 2003 Jul;185(13):3745-52
references.pmid: 12813067
references.citation: J Bacteriol. 2000 Feb;182(3):637-46
references.pmid: 10633096
references.citation: Biochemistry. 2002 Jan 8;41(1):1-7
references.pmid: 11771996
references.citation: Biomol NMR Assign. 2013 Apr;7(1):43-6
references.pmid: 22415545
references.citation: Proc Natl Acad Sci U S A. 1994 Oct 11;91(21):9876-80
references.pmid: 7937909
references.citation: PLoS One. 2013;8(3):e58769
references.pmid: 23516555
references.citation: Biochemistry. 1997 Oct 21;36(42):13043-53
references.pmid: 9335566
references.citation: Annu Rev Microbiol. 2011;65:37-55
references.pmid: 21639785
references.citation: Proc Natl Acad Sci U S A. 2009 Jan 27;106(4):1045-50
references.pmid: 19144921
references.citation: J Biomol NMR. 1994 Mar;4(2):171-80
references.pmid: 8019132