title: GABAA receptor subunit composition and competition at synapses are tuned by GABAB receptor activity.
abstract: GABABRs have a well-established role in controlling neuronal excitability and presynaptic neurotransmitter release. We examined the role of GABABR activity in modulating the number and lateral diffusion of GABAARs at inhibitory synapses. Changes in diffusion of GABAARs at synapses were observed when subunit heterogeneity was taken into account. While α1-GABAARs were unaffected, α2- and α5-GABAARs showed inverse changes in enrichment and diffusion. The intracellular TM3-4 loop of α2 was sufficient to observe the changes in diffusion by GABABR activity, whereas the loop of α5 was not. The opposing effect on α2- and α5-GABAARs was caused by a competition between GABAARs for binding slots at synapses. Receptor immobilization by cross-linking revealed that α5-GABAAR trapping at synapses is regulated by modulation of α2-GABAAR mobility. Finally, PKC activity was determined to be part of the signaling pathway through which GABABR activity modulates α2-GABAAR diffusion at synapses. These results outline a novel mechanism for tuning inhibitory transmission in a subunit-specific manner, and for the first time describe competition between GABAARs with different subunit compositions for binding slots at synapses.
authors.affiliation: Institut de Biologie de l'ENS (IBENS), 46 rue d'Ulm, 75005 Paris, France.
authors.forename: K
authors.initials: K
authors.lastname: Gerrow
authors.affiliation: Institut de Biologie de l'ENS (IBENS), 46 rue d'Ulm, 75005 Paris, France. Electronic address: triller@biologie.ens.fr.
authors.forename: A
authors.initials: A
authors.lastname: Triller
mesh_terms: D000818:Animals; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism; D051379:Mice; D009474:Neurons / Q000378:metabolism / Q000502:physiology; D011493:Protein Kinase C / Q000378:metabolism; D055503:Protein Multimerization; D017434:Protein Structure, Tertiary; D021122:Protein Subunits / Q000737:chemistry / Q000378:metabolism; D021381:Protein Transport; D011963:Receptors, GABA-A / Q000737:chemistry / Q000378:metabolism*; D018080:Receptors, GABA-B / Q000737:chemistry / Q000378:metabolism*; D013569:Synapses / Q000378:metabolism* / Q000502:physiology; D009435:Synaptic Transmission
chemical_list: D021122:Protein Subunits; D011963:Receptors, GABA-A; D018080:Receptors, GABA-B; D011493:Protein Kinase C
keywords: GABA(A)R; Inhibitory synapse; Lateral diffusion; PKC