title: Mucosal immunization of calves with recombinant bovine adenovirus-3 coexpressing truncated form of bovine herpesvirus-1 gD and bovine IL-6.
abstract: Previous studies have suggested an important role of the cytokine adjuvant IL-6 in the induction of mucosal immune responses in animals, including mice. Here, we report the in vivo ability of bovine adenovirus (BAdV)-3 expressing bovine (Bo) IL-6, to influence the systemic and mucosal immune responses against bovine herpesvirus (BHV)-1 gDt in calves. To co-express both antigen and cytokine, we first constructed a recombinant BAdV-3 expressing chimeric gDt.BoIL-6 protein (BAV326). Secondly, we constructed another recombinant BAdV-3 simultaneously expressing gDt and BoIL-6 using IRES containing a bicistronic cassette gDt-IRES.IL-6, (BAV327). Recombinant proteins expressed by BAV326 and BAV327 retained antigenicity (gDt) and biological activity (BoIL-6). Intranasal immunization of calves with recombinant BAV326, BAV327 or BAV308 (gDt alone) resulted in demonstrable levels of gDt-specific IgG responses in sera and IgA response in nasal secretions, in all animals. In addition, all calves developed complement-independent neutralizing antibody responses against BHV-1. However, no significant difference could be observed in the induction of systemic or mucosal immune response in animals immunized with recombinant BAV326 or BAV327 co-expressing BoIL-6. Moreover, there was no difference in the protection against BHV-1 challenge particularly in the amount of virus excretion in the nasal cavity in calves immunized with BAV326, BAV327 or BAV308. These data suggest that the BoIL-6 had no modulating effect on the induction of gDt specific mucosal and systemic immune responses in calves.
authors.affiliation: VIDO-InterVac, University of Saskatchewan, Saskatoon, SK, Canada.
authors.forename: Pankaj
authors.initials: P
authors.lastname: Kumar
authors.affiliation: VIDO-InterVac, University of Saskatchewan, Saskatoon, SK, Canada; Veterinary Microbiology, University of Saskatchewan, Saskatoon, SK, Canada.
authors.forename: Lisanework E
authors.initials: LE
authors.lastname: Ayalew
authors.affiliation: VIDO-InterVac, University of Saskatchewan, Saskatoon, SK, Canada.
authors.forename: Dale L
authors.initials: DL
authors.lastname: Godson
authors.affiliation: VIDO-InterVac, University of Saskatchewan, Saskatoon, SK, Canada; Veterinary Microbiology, University of Saskatchewan, Saskatoon, SK, Canada.
authors.forename: Amit
authors.initials: A
authors.lastname: Gaba
authors.affiliation: VIDO-InterVac, University of Saskatchewan, Saskatoon, SK, Canada; Veterinary Microbiology, University of Saskatchewan, Saskatoon, SK, Canada.
authors.forename: Lorne A
authors.initials: LA
authors.lastname: Babiuk
authors.affiliation: VIDO-InterVac, University of Saskatchewan, Saskatoon, SK, Canada; Veterinary Microbiology, University of Saskatchewan, Saskatoon, SK, Canada; Vaccinology & Immunotherapeutics Program, School of Public Health, University of Saskatchewan, Saskatoon, SK, Canada. Electronic address: Suresh.Tik@usask.ca.
authors.forename: Suresh K
authors.initials: SK
authors.lastname: Tikoo
mesh_terms: D000256:Adenoviridae; D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D000917:Antibody Formation; D002417:Cattle; D002418:Cattle Diseases / Q000517:prevention & control* / Q000821:virology; D005822:Genetic Vectors; D006566:Herpesviridae Infections / Q000517:prevention & control / Q000662:veterinary*; D007242:Herpesvirus 1, Bovine; D018928:Immunity, Mucosal; D007071:Immunoglobulin A, Secretory / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood; D015850:Interleukin-6 / Q000276:immunology*; D009500:Neutralization Tests; D011994:Recombinant Proteins / Q000276:immunology; D014764:Viral Proteins / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D007071:Immunoglobulin A, Secretory; D007074:Immunoglobulin G; D015850:Interleukin-6; D011994:Recombinant Proteins; D014764:Viral Proteins; D014765:Viral Vaccines; C046998:bovine herpesvirus type-1 glycoproteins
keywords: Bovine IL-6; Bovine adenovirus; Bovine herpesvirus; Mucosal immunization; gDt