title: Identification and characterisation of a novel 1-Cys thioredoxin peroxidase gene (AccTpx5) from Apis cerana cerana.
abstract: Thioredoxin peroxidases (Tpxs), members of the antioxidant protein family, play critical roles in resisting oxidative stress. In this work, a novel 1-Cys thioredoxin peroxidase gene was isolated from Apis cerana cerana and was named AccTpx5. The open reading frame (ORF) of AccTpx5 is 663bp in length and encodes a 220-amino acid protein with a predicted molecular mass and isoelectric point of 24,921kDa and 5.45, respectively. Promoter sequence analysis of AccTpx5 revealed the presence of putative transcription factor binding sites related to early development and stress responses. Additionally, real-time quantitative PCR (Q-PCR) analysis indicated that AccTpx5 was primarily present in some developmental stages, with the highest expression levels in the first-instar larvae. The expression level of AccTpx5 was up-regulated under various abiotic stresses, including 4°C, 42°C, HgCl2, H2O2, phoxim and acaricide treatments. Conversely, it was down-regulated by UV and pyriproxyfen treatments. Moreover, H2O2 concentration dramatically increased under a variety of stressful conditions. Finally, the purified recombinant AccTpx5 protein protected the supercoiled form of plasmid DNA from damage in the thiol-dependent mixed-function oxidation (MFO) system. These results suggest that AccTpx5 most likely plays an essential role in antioxidant defence.
authors.affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Yan
authors.affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China; School of Basic Medical Sciences, Taishan Medical University, Taian, Shandong 271000, PR China.
authors.forename: Yuanying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China.
authors.forename: Yifeng
authors.initials: Y
authors.lastname: Huaxia
authors.affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China.
authors.forename: Xiuling
authors.initials: X
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China.
authors.forename: Pengbo
authors.initials: P
authors.lastname: Yao
authors.affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China. Electronic address: xqguo@sdau.edu.cn.
authors.forename: Xingqi
authors.initials: X
authors.lastname: Guo
authors.affiliation: College of Animal Science and Technology, Shandong Agricultural University, Taian, Shandong 271018, PR China. Electronic address: bhxu@sdau.edu.cn.
authors.forename: Baohua
authors.initials: B
authors.lastname: Xu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001483:Base Sequence; D001516:Bees / Q000187:drug effects / Q000235:genetics* / Q000502:physiology / Q000528:radiation effects; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism; D007605:Juvenile Hormones / Q000494:pharmacology; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D009946:Organothiophosphorus Compounds / Q000494:pharmacology; D018384:Oxidative Stress; D054464:Peroxiredoxins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D010802:Phylogeny; D011487:Protein Conformation; D011725:Pyridines / Q000494:pharmacology; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013312:Stress, Physiological; D014466:Ultraviolet Rays
chemical_list: D000975:Antioxidants; D019476:Insect Proteins; D007605:Juvenile Hormones; D009946:Organothiophosphorus Compounds; D011725:Pyridines; D011994:Recombinant Proteins; C055613:pyriproxyfen; C003135:phoxim; D054464:Peroxiredoxins
keywords: Abiotic stress; Antioxidant defence; Apis cerana cerana; Q-PCR; Thioredoxin peroxidases 5