title: Silk screen based dual spin-filter module for perfusion culture of adherent and non-adherent mammalian cells.
abstract: Spin-filters have been primarily used for producing therapeutic proteins from mammalian cells. However, disposability and/or high filter clogging of the existing spin-filter systems affect the process economy and productivity. Hence, to address these drawbacks a reusable dual spin-filter module for perfusion culture of adherent and non-adherent mammalian cells was designed. Two non-woven Bombyx mori silk layers were used as filter screen; the outer layer was conducive to cell attachment whilst the inner was non-conducive. Adherent cells can be cultured either in suspended mode using its inner single module or as monolayer of cells using its dual concentric module. We achieved 30 % higher urokinase productivity as compared to the stainless-steel spin-filter during perfusion experiments of adherent human kidney cells in suspended mode. This was due to the hydrophobic and negatively-charged silk screen that allows clog-free perfusion culture for prolonged periods.
authors.affiliation: Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, Hauz Khas, Block 1, Room No. I-138, New Delhi, 110016, India.
authors.forename: Shweta
authors.initials: S
authors.lastname: Kamthan
authors.forename: James
authors.initials: J
authors.lastname: Gomes
authors.forename: Pradip K
authors.initials: PK
authors.lastname: Roychoudhury
mesh_terms: D000818:Animals; D061249:Batch Cell Culture Techniques; D012831:Bombyx; D002448:Cell Adhesion; D002452:Cell Count; D018929:Cell Culture Techniques / Q000295:instrumentation*; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival; D006801:Humans; D008322:Mammals / Q000378:metabolism*; D010477:Perfusion / Q000295:instrumentation* / Q000379:methods*; D011108:Polymers / Q000737:chemistry; D047011:Silk / Q000737:chemistry* / Q000648:ultrastructure; D013193:Stainless Steel; D013499:Surface Properties; D013997:Time Factors
chemical_list: D011108:Polymers; D047011:Silk; D013193:Stainless Steel