title: Simple and rapid quality control of sulfated glycans by a fluorescence sensor assay--exemplarily developed for the sulfated polysaccharides from red algae Delesseria sanguinea.
abstract: Sulfated polysaccharides (SP) from algae are of great interest due to their manifold biological activities. Obstacles to commercial (especially medical) application include considerable variability and complex chemical composition making the analysis and the quality control challenging. The aim of this study was to evaluate a simple microplate assay for screening the quality of SP. It is based on the fluorescence intensity (FI) increase of the sensor molecule Polymer-H by SP and was originally developed for direct quantification of SP. Exemplarily, 65 SP batches isolated from the red alga Delesseria sanguinea (D.s.-SP) and several other algae polysaccharides were investigated. Their FI increase in the Polymer-H assay was compared with other analytical parameters. By testing just one concentration of a D.s.-SP sample, quality deviations from the reference D.s.-SP and thus both batch-to-batch variability and stability can be detected. Further, structurally distinct SP showed to differ in their concentration-dependent FI profiles. By using corresponding reference compounds, the Polymer-H assay is therefore applicable as identification assay with high negative predictability. In conclusion, the Polymer-H assay showed to represent not only a simple method for quantification, but also for characterization identification and differentiation of SP of marine origin.
authors.affiliation: Pharmaceutical Institute, Pharmaceutical Biology, Christian-Albrechts-University of Kiel, Gutenbergstr. 76, D-24118 Kiel, Germany. sluehn@pharmazie.uni-kiel.de.
authors.forename: Susanne
authors.initials: S
authors.lastname: Lühn
authors.affiliation: Pharmaceutical Institute, Pharmaceutical Biology, Christian-Albrechts-University of Kiel, Gutenbergstr. 76, D-24118 Kiel, Germany. jgrimm@pharmazie.uni-kiel.de.
authors.forename: Juliane C
authors.initials: JC
authors.lastname: Grimm
authors.affiliation: Pharmaceutical Institute, Pharmaceutical Biology, Christian-Albrechts-University of Kiel, Gutenbergstr. 76, D-24118 Kiel, Germany. salban@pharmazie.uni-kiel.de.
authors.forename: Susanne
authors.initials: S
authors.lastname: Alban
mesh_terms: D005453:Fluorescence; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000592:standards; D011786:Quality Control; D000461:Rhodophyta / Q000737:chemistry*
chemical_list: D011134:Polysaccharides
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