title: [Isolation, culture and ultrastructure analysis of mesenchymal stem cells from human periodontal ligament].
abstract: OBJECTIVE
  To isolate and culture human periodontal ligament stem cells (PDLSCs) and observe its ultrastructure.
  
  
  METHODS
  The proliferation and growth characteristics of human periodontal ligament cells were observed in primary culture and colony culture. PDLSCs were isolated by magnetic activated cell sorting (MACS) and ultrastructural characterization was observed by electron microscopy.
  
  
  RESULTS
  When the cells were cultured at low density, PDLSCs grew in a colony-like manner. With the exception of a small amount of rough endoplasmic reticulum, ribosomes, and mitochondria, relatively few organelles were found in the cytoplasm, suggesting that they had remained undifferentiated.
  
  
  CONCLUSIONS
  PDLSCs showed colony-like growth capacity and had ultrastructural characterization with stem cells. This indicated that PDLSCs could act as the appropriate seed cells for cell-based periodontal tissue regeneration.
authors.affiliation: Department of Periodontology, Peking University School and Hospital of Stomatology, Beijing 100081, China; Department of Periodontology, Stomatological Hospital, Capital Medical University, Beijing, 100050, China.
authors.forename: Feng-qiu
authors.initials: FQ
authors.lastname: Zhang
authors.affiliation: Department of Periodontology, Peking University School and Hospital of Stomatology, Beijing 100081, China.
authors.forename: Huan-xin
authors.initials: HX
authors.lastname: Meng
authors.affiliation: Department of Periodontology, Peking University School and Hospital of Stomatology, Beijing 100081, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Han
authors.affiliation: Department of Periodontology, Stomatological Hospital, Capital Medical University, Beijing, 100050, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Ding
mesh_terms: D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002469:Cell Separation; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000648:ultrastructure*; D010513:Periodontal Ligament / Q000166:cytology*