title: Exopolysaccharides isolated from hydrothermal vent bacteria can modulate the complement system.
abstract: The complement system is involved in the defence against bacterial infection, or in the elimination of tumour cells. However, disturbances in this system contributes to the pathogenesis of various inflammatory diseases. The efficiency of therapeutic anti-tumour antibodies is enhanced when the complement system is stimulated. In contrast, cancer cells are able to inhibit the complement system and thus proliferate. Some marine molecules are currently being developed as new drugs for use in humans. Among them, known exopolyssacharides (EPSs) generally originate from fungi, but few studies have been performed on bacterial EPSs and even fewer on EPSs extracted from deep-sea hydrothermal vent microbes. For use in humans, these high molecular weight EPSs must be depolymerised. Furthermore, the over-sulphation of EPSs can modify their biological activity. The aim of this study was to investigate the immunodulation of the complement system by either native or over-sulphated low molecular weight EPSs isolated from vent bacteria in order to find pro or anti-activators of complement.
authors.affiliation: Biotechnology and Marine Molecules Laboratory, IFREMER, Brest, France; Cellular Therapy and Immunobiology of Cancer Laboratory, University Hospital of Brest, Brest, France.
authors.forename: Anthony
authors.initials: A
authors.lastname: Courtois
authors.affiliation: Cellular Therapy and Immunobiology of Cancer Laboratory, University Hospital of Brest, Brest, France.
authors.forename: Christian
authors.initials: C
authors.lastname: Berthou
authors.affiliation: Biotechnology and Marine Molecules Laboratory, IFREMER, Brest, France.
authors.forename: Jean
authors.initials: J
authors.lastname: Guézennec
authors.affiliation: Biotechnology and Marine Molecules Laboratory, IFREMER, Brest, France.
authors.forename: Claire
authors.initials: C
authors.lastname: Boisset
authors.affiliation: Cellular Therapy and Immunobiology of Cancer Laboratory, University Hospital of Brest, Brest, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Bordron
mesh_terms: D001419:Bacteria / Q000737:chemistry*; D003167:Complement Activation / Q000187:drug effects / Q000276:immunology; D015922:Complement C1q / Q000276:immunology; D003171:Complement Pathway, Classical / Q000187:drug effects / Q000276:immunology; D003165:Complement System Proteins / Q000276:immunology*; D006801:Humans; D060086:Hydrothermal Vents / Q000382:microbiology*; D056747:Immunomodulation / Q000187:drug effects*; D008970:Molecular Weight; D011135:Polysaccharides, Bacterial / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D011135:Polysaccharides, Bacterial; D015922:Complement C1q; D003165:Complement System Proteins
references.citation: Calcif Tissue Int. 2003 Sep;73(3):232-6
references.pmid: 14667135
references.citation: Int J Mol Sci. 2012 Oct 30;13(11):14002-15
references.pmid: 23203046
references.citation: J Appl Microbiol. 1997 Apr;82(4):422-30
references.pmid: 9134716
references.citation: Trends Biotechnol. 2004 Oct;22(10):524-30
references.pmid: 15450746
references.citation: J Biotechnol. 2000 Jan 28;77(1):123-35
references.pmid: 10674219
references.citation: Thromb Res. 2008;122(1):109-16
references.pmid: 17936880
references.citation: Int Immunopharmacol. 2001 Mar;1(3):495-506
references.pmid: 11367533
references.citation: J Immunol. 1997 Feb 1;158(3):1324-31
references.pmid: 9013976
references.citation: FEBS Lett. 1991 Apr 9;281(1-2):55-8
references.pmid: 2015909
references.citation: Infect Immun. 1986 Sep;53(3):480-5
references.pmid: 3744547
references.citation: Eur J Immunol. 2012 Apr;42(4):993-1004
references.pmid: 22531923
references.citation: Cancer Res. 2011 Mar 15;71(6):2298-307
references.pmid: 21252115
references.citation: Arch Biochem Biophys. 1999 Jan 15;361(2):215-22
references.pmid: 9882449
references.citation: Carbohydr Res. 1999 Nov 23;322(1-2):40-5
references.pmid: 10629947
references.citation: Carbohydr Res. 1996 Aug 19;289:201-8
references.pmid: 8805780
references.citation: Biochemistry. 2005 Feb 22;44(7):2602-9
references.pmid: 15709773
references.citation: Annu Rev Biochem. 1969;38:389-414
references.pmid: 4184995
references.citation: Exp Oncol. 2006 Dec;28(4):308-13
references.pmid: 17285116
references.citation: J Immunol. 2011 Oct 15;187(8):4369-73
references.pmid: 21930969
references.citation: J Dairy Sci. 2006 Aug;89(8):2873-81
references.pmid: 16840603
references.citation: Mar Drugs. 2008;6(3):407-17
references.pmid: 19005576
references.citation: Front Immunol. 2012 Mar 05;3:38
references.pmid: 22566921
references.citation: Am J Pathol. 2007 Sep;171(3):715-27
references.pmid: 17640961
references.citation: Biochim Biophys Acta. 2001 Oct 3;1528(2-3):141-51
references.pmid: 11687301
references.citation: Annu Rev Biochem. 1988;57:321-47
references.pmid: 3052276
references.citation: Biotechnol Prog. 2005 Nov-Dec;21(6):1644-52
references.pmid: 16321047
references.citation: Biomaterials. 1996 Mar;17(6):597-603
references.pmid: 8652778
references.citation: Calcif Tissue Int. 2003 Jan;72(1):74-9
references.pmid: 12370797
references.citation: Curr Opin Chem Biol. 2001 Oct;5(5):572-7
references.pmid: 11578932
references.citation: Blood. 1996 Jul 1;88(1):184-93
references.pmid: 8704173
references.citation: Clin Microbiol Rev. 2000 Oct;13(4):523-33
references.pmid: 11023954
references.citation: Immunohematology. 2004;20(1):3-22
references.pmid: 15373665
references.citation: Anal Biochem. 1994 Aug 1;220(2):244-8
references.pmid: 7978265
references.citation: Glycobiology. 2003 Dec;13(12):29G-30G
references.pmid: 14655675
references.citation: Int J Syst Bacteriol. 1997 Oct;47(4):989-95
references.pmid: 9336897
references.citation: Biochimie. 2001 Jul;83(7):557-63
references.pmid: 11522383
references.citation: Transplant Proc. 1992 Apr;24(2):559-62
references.pmid: 1566430
references.citation: Curr Opin Immunol. 2001 Feb;13(1):74-8
references.pmid: 11154921
references.citation: Pharmacol Rev. 1998 Mar;50(1):59-87
references.pmid: 9549758
references.citation: Clin Exp Rheumatol. 2003 Mar-Apr;21(2):225-8
references.pmid: 12747280
references.citation: Int Immunopharmacol. 2006 Jan;6(1):8-13
references.pmid: 16332508
references.citation: J Biol Chem. 1951 Nov;193(1):265-75
references.pmid: 14907713