title: Sizing up the poly(A) tail: insights from deep sequencing.
abstract: Global investigation of poly(A) tails has been hindered by technical challenges. In a recent advance, two groups developed deep sequencing methods to globally interrogate poly(A) tail length and sequence with high precision, opening new avenues for investigation of poly(A) tail functions in mRNA metabolism. Initial applications of these methods reveal insights into the relationship between poly(A) tail length and translational efficiency, and identify widespread uridylation and guanylation at the 3' ends of transcripts.
authors.affiliation: Department of Biochemistry and Molecular Biology, Rutgers New Jersey Medical School, Newark, NJ 07103.
authors.forename: Dinghai
authors.initials: D
authors.lastname: Zheng
authors.affiliation: Department of Biochemistry and Molecular Biology, Rutgers New Jersey Medical School, Newark, NJ 07103. Electronic address: btian@rutgers.edu.
authors.forename: Bin
authors.initials: B
authors.lastname: Tian
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics*; D000818:Animals; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D011061:Poly A / Q000235:genetics*; D012323:RNA Processing, Post-Transcriptional; D012333:RNA, Messenger / Q000235:genetics*
chemical_list: D020413:3' Untranslated Regions; D012333:RNA, Messenger; D011061:Poly A
keywords: mRNA stability; poly(A) tail; polyadenylation; post-transcriptional control; translation
references.citation: J Biol Chem. 1993 Nov 5;268(31):22955-8
references.pmid: 8226806
references.citation: Science. 2012 Apr 13;336(6078):233-7
references.pmid: 22422859
references.citation: Nature. 2010 Aug 12;466(7308):835-40
references.pmid: 20703300
references.citation: Nature. 2014 Apr 3;508(7494):66-71
references.pmid: 24476825
references.citation: RNA. 2007 Jul;13(7):982-97
references.pmid: 17586758
references.citation: Mol Cell. 2014 Mar 20;53(6):1044-52
references.pmid: 24582499
references.citation: Wiley Interdiscip Rev RNA. 2011 Mar-Apr;2(2):167-83
references.pmid: 21957004
references.citation: BMC Genomics. 2012 Jul 24;13:341
references.pmid: 22827831
references.citation: Nucleic Acids Res. 2007;35(19):e132
references.pmid: 17933768
references.citation: Nat Struct Mol Biol. 2012 Jun 05;19(6):577-85
references.pmid: 22664985