title: Regulation of branching dynamics by axon-intrinsic asymmetries in Tyrosine Kinase Receptor signaling.
abstract: Axonal branching allows a neuron to connect to several targets, increasing neuronal circuit complexity. While axonal branching is well described, the mechanisms that control it remain largely unknown. We find that in the Drosophila CNS branches develop through a process of excessive growth followed by pruning. In vivo high-resolution live imaging of developing brains as well as loss and gain of function experiments show that activation of Epidermal Growth Factor Receptor (EGFR) is necessary for branch dynamics and the final branching pattern. Live imaging also reveals that intrinsic asymmetry in EGFR localization regulates the balance between dynamic and static filopodia. Elimination of signaling asymmetry by either loss or gain of EGFR function results in reduced dynamics leading to excessive branch formation. In summary, we propose that the dynamic process of axon branch development is mediated by differential local distribution of signaling receptors. DOI: http://dx.doi.org/10.7554/eLife.01699.001.
authors.affiliation: Center for the Biology of Disease, Vlaams Instituut voor Biotechnologie, Leuven, Belgium.
authors.forename: Marlen
authors.initials: M
authors.lastname: Zschätzsch
authors.forename: Carlos
authors.initials: C
authors.lastname: Oliva
authors.forename: Marion
authors.initials: M
authors.lastname: Langen
authors.forename: Natalie
authors.initials: N
authors.lastname: De Geest
authors.forename: Mehmet Neset
authors.initials: MN
authors.lastname: Ozel
authors.forename: W Ryan
authors.initials: WR
authors.lastname: Williamson
authors.forename: William C
authors.initials: WC
authors.lastname: Lemon
authors.forename: Alessia
authors.initials: A
authors.lastname: Soldano
authors.forename: Sebastian
authors.initials: S
authors.lastname: Munck
authors.forename: P Robin
authors.initials: PR
authors.lastname: Hiesinger
authors.forename: Natalia
authors.initials: N
authors.lastname: Sanchez-Soriano
authors.forename: Bassem A
authors.initials: BA
authors.lastname: Hassan
mesh_terms: D000818:Animals; D001369:Axons / Q000502:physiology*; D004330:Drosophila; D029721:Drosophila Proteins / Q000378:metabolism; D066246:ErbB Receptors / Q000378:metabolism; D009473:Neuronal Plasticity; D061848:Optical Imaging; D020794:Receptor Protein-Tyrosine Kinases / Q000378:metabolism*; D018010:Receptors, Invertebrate Peptide / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D029721:Drosophila Proteins; D018010:Receptors, Invertebrate Peptide; C066532:Egfr protein, Drosophila; D066246:ErbB Receptors; D020794:Receptor Protein-Tyrosine Kinases
keywords: axonal branching; brain development; signaling
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