title: Bioconjugation of poly(acrylic acid)-capped BaYF5:Yb3+/Er3+ up-conversion nanoparticles to bovine serum albumin: synthesis and photoluminescent properties.
abstract: Water-soluble BaYF5:Yb3+/Er3+ nanoparticles with the surface functionalized by a layer of poly(acrylic acid) (PAA) were synthesized via a facile one-step PAA-assisted hydrothermal method. Bovine serum albumin (BSA) protein was conjugated with BaYF5:Yb3+/Er3+ upconversion nanoparticles via free carboxylic acid groups on the surface of nanoparticles. The final products were characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), infrared (IR) spectrophotometry, ultraviolet (UV) spectrophotometry and photoluminescence spectroscopy (PL). The XRD results showed that PAA-capped BaYF5:Yb3+/Er3+ upconversion nanoparticles could be obtained via a PAA assisted hydrothermal process with the pH value of 8 at 200 degrees C for 24 h. The TEM results showed that the morphology of BaYF5:Yb3+/Er3+ nanoparticles was spherical particles with an average diameter of about 4 nm. The IR and UV spectra showed that BSA has been conjugated with BaYF5:Yb3+/Er3+ up-conversion nanoparticles. The luminescence properties of BaYF5:Yb3+/Er3+ up-conversion nanoparticles were also studied. The luminescence properties of the products suggest that BaYF5:Yb3+/Er3+ upconversion nanoparticles have promising applications for labels in biological assays.
authors.forename: Wanyue
authors.initials: W
authors.lastname: Shao
authors.forename: Zhengang
authors.initials: Z
authors.lastname: Sun
authors.forename: Ruinian
authors.initials: R
authors.lastname: Hua
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Jun
authors.initials: J
authors.lastname: Zhao
authors.forename: Liyan
authors.initials: L
authors.lastname: Na
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D004871:Erbium / Q000737:chemistry*; D049449:Luminescence; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles; D033362:Powder Diffraction; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D015018:Ytterbium / Q000737:chemistry*
chemical_list: D000180:Acrylic Resins; D012710:Serum Albumin, Bovine; C006903:carbopol 940; D004871:Erbium; D015018:Ytterbium