title: Investigation of a new core-shell particle column for ion-pair reversed-phase liquid chromatography analysis of oligonucleotides.
abstract: A new core-shell particle column showed excellent performance and durability for separation of short (∼21-mer) ribonucleic acid (RNA) oligonucleotides by ion-pair reversed-phase liquid chromatography (IP-RPLC). Previously investigated core-shell C18 columns showed excellent peak shapes and separations of closely eluting impurities by IP-RPLC. However, these columns showed only modest long-term stability at the neutral pH and elevated column temperatures of ≥60°C, typically used for IP-RPLC analysis of oligonucleotides. The newly introduced SunShell C18 column provided separations comparable to the previously evaluated core-shell columns, but with significantly improved long-term column stability when operated at neutral pH and elevated column temperature.
authors.affiliation: Merck Research Laboratories, Rahway, NJ 07065, USA; Department of Chemistry, Drexel University, Philadelphia, PA 19101, USA. Electronic address: mirlinda_biba@merck.com.
authors.forename: Mirlinda
authors.initials: M
authors.lastname: Biba
authors.affiliation: Merck Research Laboratories, Rahway, NJ 07065, USA.
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Welch
authors.affiliation: Department of Chemistry, Drexel University, Philadelphia, PA 19101, USA.
authors.forename: Joe P
authors.initials: JP
authors.lastname: Foley
mesh_terms: D056148:Chromatography, Reverse-Phase / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D009841:Oligonucleotides / Q000032:analysis* / Q000737:chemistry; D013696:Temperature; D013997:Time Factors
chemical_list: D009841:Oligonucleotides
keywords: Column stability; Core–shell particle column; High temperature RPLC; Ion-pair reversed-phase liquid chromatography; Oligonucleotides