title: Influence of dilute acid and alkali pretreatment on reducing sugar production from corncobs by crude enzymatic method: a comparative study.
abstract: In the present study, two commonly used catalysts in chemical pretreatment, sulfuric acid and sodium hydroxide, were tested to evaluate the effect of solid-to-liquid ratio on pretreatment and enzymatic hydrolysis. Solid to liquid ratio (S/L) was influential on sugars released with an increase in the S/L ratio between 0.03 and 0.2. Enzymatic digestibility of 0.25 M H2SO4 pretreated corncobs were released more sugars (415.12 mg/mL); whereas, corncobs pretreated with NaOH released 350.12 mg/mL of reducing sugars at S/L 0.05. Further, in comparison with NaOH pretreated corncobs, acid treated material substantially increased the accessibility and digestibility of cellulose during crude enzymatic hydrolysis (28.96 FPU) and released 398.95 mg/mL reducing sugars.
authors.affiliation: Department of Biotechnology, National Institute of Technology, Warangal 506004, India.
authors.forename: Rama Raju
authors.initials: RR
authors.lastname: Baadhe
authors.affiliation: Department of Chemical Engineering, Monash University, Clayton, Victoria 3800, Australia. Electronic address: pravichandra@gmail.com.
authors.forename: Ravichandra
authors.initials: R
authors.lastname: Potumarthi
authors.affiliation: Department of Biotechnology, National Institute of Technology, Warangal 506004, India.
authors.forename: Naveen Kumar
authors.initials: NK
authors.lastname: Mekala
mesh_terms: D001709:Biotechnology / Q000379:methods*; D002241:Carbohydrates / Q000096:biosynthesis*; D002480:Cellulase / Q000378:metabolism*; D006868:Hydrolysis; D012972:Sodium Hydroxide / Q000494:pharmacology*; D013464:Sulfuric Acids / Q000494:pharmacology*; D003313:Zea mays / Q000187:drug effects* / Q000378:metabolism*
chemical_list: D002241:Carbohydrates; D013464:Sulfuric Acids; D012972:Sodium Hydroxide; D002480:Cellulase; C033158:sulfuric acid
keywords: Bioethanol; Corncobs; Enzymatic hydrolysis; Pretreatment; Reducing sugars