title: Differences in the expression of human papillomavirus type 16 (HPV-16) E6 oncogene mRNA in SiHa cell line inoculated with CMV, HSV or ureaplasmas.
abstract: One of the factors associated with an increased risk of HPV-related malignant transformation may be bacterial and/or viral infections. The aim of our study was to examine whether the presence of infectious agents commonly detected in the genitourinary tract such as herpesviruses (HSV, CMV), and ureaplasmas (Ureaplasma urealyticum, Ureaplasma parvum) may lead to alterations in the expression of the HPV-16 E6 oncogene. Quantitative RT-PCR analysis was used to assess the level of HPV-16 E6 mRNA expression in SiHa cells. The presence of HSV-1 or HSV-2 in SiHa cells caused a 1.5-fold increase in HPV-16 E6 mRNA expression as compared with non-inoculated SiHa cells. Ureaplasma urealyticum presence but not Ureaplasma parvum stimulated the expression of HPV-16 E6 resulting in a nearly five-fold (4.8) up-regulated E6 mRNA level in SiHa cells. Our study is the first to suggest that infection of Ureaplasma urealyticum in an urogenital tract could increase the risk of cervical cancer by overexpression of the HPV E6 oncogene.
authors.forename: Sława
authors.initials: S
authors.lastname: Szostek
authors.forename: Barbara
authors.initials: B
authors.lastname: Zawilińska
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Biernat-Sudolska
authors.forename: Jolanta
authors.initials: J
authors.lastname: Kopeć
authors.forename: Ewa
authors.initials: E
authors.lastname: Kłeszcz
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Koprynia
authors.forename: Danuta
authors.initials: D
authors.lastname: Rojek-Zakrzewska
authors.forename: Magdalena
authors.initials: M
authors.lastname: Kosz-Vnenchak
mesh_terms: D002460:Cell Line; D003587:Cytomegalovirus; D015967:Gene Expression Regulation, Viral / Q000502:physiology*; D024581:Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) / Q000235:genetics / Q000378:metabolism; D006801:Humans; D009856:Oncogene Proteins, Viral / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D018139:Simplexvirus; D014509:Ureaplasma
chemical_list: C068651:E6 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral; D012333:RNA, Messenger; D012097:Repressor Proteins; D024581:Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating)