title: Biotin-conjugated N-methylisatoic anhydride: a chemical tool for nucleic acid separation by selective 2'-hydroxyl acylation of RNA.
abstract: An isatoic anhydride derivative conjugated to a biotin and a disulfide linker was specifically designed for the separation of nucleic acids. Starting from a DNA-RNA mixture, a selective 2'-hydroxyl acylation of RNAs followed by capture with streptavidin-coated magnetic beads and cleavage of the disulfide led to elution of RNAs.
authors.affiliation: Normandie Univ, France.
authors.forename: S
authors.initials: S
authors.lastname: Ursuegui
authors.forename: N
authors.initials: N
authors.lastname: Chivot
authors.forename: S
authors.initials: S
authors.lastname: Moutin
authors.forename: A
authors.initials: A
authors.lastname: Burr
authors.forename: C
authors.initials: C
authors.lastname: Fossey
authors.forename: T
authors.initials: T
authors.lastname: Cailly
authors.forename: A
authors.initials: A
authors.lastname: Laayoun
authors.forename: F
authors.initials: F
authors.lastname: Fabis
authors.forename: A
authors.initials: A
authors.lastname: Laurent
mesh_terms: D000215:Acylation; D000812:Anhydrides / Q000737:chemistry*; D001710:Biotin / Q000737:chemistry*; D004247:DNA / Q000737:chemistry* / Q000302:isolation & purification*; D006878:Hydroxides / Q000737:chemistry*; D012313:RNA / Q000737:chemistry* / Q000302:isolation & purification*; D012367:RNA, Viral / Q000737:chemistry / Q000302:isolation & purification; D019809:Streptavidin / Q000737:chemistry; D062367:ortho-Aminobenzoates / Q000737:chemistry*
chemical_list: D000812:Anhydrides; D006878:Hydroxides; D012367:RNA, Viral; D062367:ortho-Aminobenzoates; C077537:N-methylisatoic anhydride; D012313:RNA; D001710:Biotin; D004247:DNA; D019809:Streptavidin; C031356:hydroxide ion