title: Effects of realistic doses of atrazine, metolachlor, and glyphosate on lipid peroxidation and diet-derived antioxidants in caged honey bees (Apis mellifera).
abstract: The decline in the population of pollinators is a worrying phenomenon worldwide. In North America, the extensive use of herbicides in maize and soya crops may affect the health of nontarget organisms like the honey bee. In this study, caged honey bees were exposed to realistic doses of atrazine, metolachlor, and glyphosate for 10 days via contaminated syrup. Peroxidation of lipids was evaluated using the thiobarbituric acid reactive substance (TBARS) test, and diet-derived antioxidants-carotenoids, all-trans-retinol (at-ROH) and α-tocopherol-were detected and quantified using reversed-phase HPLC techniques. Significant increases in syrup consumption were observed in honey bees exposed to metolachlor, and a lower TBARS value was recorded for the highest dose. No relationship was observed between the peroxidation of lipids and the levels of antioxidants. However, β-carotene, which was found to be the most abundant carotenoid, and at-ROH (derived from β-carotene) both decreased with increasing doses of atrazine and glyphosate. In contrast, metolachlor increased levels of at-ROH without any effects on β-carotene. These results show that the honey bee carotenoid-retinoid system may be altered by sublethal field-realistic doses of herbicides.
authors.affiliation: Département des Sciences Biologiques, Université du Québec à Montréal, C.P. 8888, Succursale Centre-Ville, Montréal, QC, H3C 3P8, Canada.
authors.forename: Stephanie Hedrei
authors.initials: SH
authors.lastname: Helmer
authors.forename: Anahi
authors.initials: A
authors.lastname: Kerbaol
authors.forename: Philippe
authors.initials: P
authors.lastname: Aras
authors.forename: Catherine
authors.initials: C
authors.lastname: Jumarie
authors.forename: Monique
authors.initials: M
authors.lastname: Boily
mesh_terms: D000081:Acetamides / Q000633:toxicity; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001280:Atrazine / Q000633:toxicity; D001516:Bees / Q000187:drug effects*; D002338:Carotenoids / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D005998:Glycine / Q000031:analogs & derivatives / Q000633:toxicity; D006540:Herbicides / Q000633:toxicity*; D015227:Lipid Peroxidation / Q000187:drug effects*; D018384:Oxidative Stress / Q000187:drug effects*; D018709:Statistics, Nonparametric; D017392:Thiobarbituric Acid Reactive Substances; D024502:alpha-Tocopherol / Q000378:metabolism; D000097797:Glyphosate
chemical_list: D000081:Acetamides; D000975:Antioxidants; D001280:Atrazine; D002338:Carotenoids; D005998:Glycine; D006540:Herbicides; D017392:Thiobarbituric Acid Reactive Substances; D024502:alpha-Tocopherol; D000097797:Glyphosate; C051786:metolachlor
references.citation: J Appl Toxicol. 2010 Aug;30(6):582-9
references.pmid: 20809547
references.citation: Vision Res. 1999 Jan;39(2):219-29
references.pmid: 10326132
references.citation: Anal Biochem. 2008 Jul 1;378(1):71-9
references.pmid: 18410739
references.citation: PLoS One. 2013;8(1):e54092
references.pmid: 23382869
references.citation: PLoS One. 2013 Aug 05;8(8):e72016
references.pmid: 23940803
references.citation: Acta Trop. 2007 Sep;103(3):222-30
references.pmid: 17686447
references.citation: PLoS One. 2012;7(1):e29268
references.pmid: 22235278
references.citation: Molecules. 2011 Jan 25;16(2):1055-69
references.pmid: 21350393
references.citation: Chem Rev. 2014 Jan 8;114(1):1-125
references.pmid: 24266866
references.citation: Sci Total Environ. 2007 Aug 1;381(1-3):134-45
references.pmid: 17467774
references.citation: Environ Pollut. 2009 Nov;157(11):2903-27
references.pmid: 19500891
references.citation: Curr Biol. 2010 Mar 9;20(5):458-63
references.pmid: 20189388
references.citation: J Insect Physiol. 2007 Aug;53(8):760-73
references.pmid: 17532002
references.citation: Curr Biol. 2010 Feb 9;20(3):R96-8
references.pmid: 20144777
references.citation: Anal Biochem. 1976 May 7;72:248-54
references.pmid: 942051
references.citation: Interdiscip Toxicol. 2012 Sep;5(3):133-40
references.pmid: 23554553
references.citation: Biogerontology. 2014 Apr;15(2):129-37
references.pmid: 24305779
references.citation: PLoS One. 2010 Mar 19;5(3):e9754
references.pmid: 20333298
references.citation: BMC Complement Altern Med. 2012 Apr 18;12:45
references.pmid: 22513008