title: Comparative Assessment of Factors Involved in Acetoin Synthesis by Bacillus subtilis 168.
abstract: Acetoin is widely used as flavor agent and serves as a precursor for chemical synthesis. Here we focused on identifying the best physiological conditions (initial substrate concentrations, pH, temperature, and agitation) for enhanced acetoin accumulation by Bacillus subtilis 168. The optimal physiological conditions support maximum acetoin accumulation by minimizing byproduct (acetate and butanediol) synthesis and a maximum of 75% enhancement in acetoin yield could be achieved. Additionally, the effect of change in ALS (acetolactate synthase) and ALDC (acetolactate decarboxylase) activities was evaluated on acetoin accumulation. Increasing ALS and ALDC enzyme activities led to efficient utilization of pyruvate towards acetoin accumulation and about 80% enhancement in acetoin accumulation was observed.
authors.affiliation: Department of Bioscience and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India.
authors.forename: Pratibha
authors.initials: P
authors.lastname: Sharma
authors.affiliation: Department of Chemical Engineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India.
authors.forename: Santosh
authors.initials: S
authors.lastname: Noronha
references.citation: J Bacteriol. 1995 Sep;177(18):5261-9
references.pmid: 7665514
references.citation: Metab Eng. 2003 Apr;5(2):133-49
references.pmid: 12850135
references.citation: Cell. 2009 Dec 24;139(7):1366-75
references.pmid: 20064380
references.citation: Appl Environ Microbiol. 2009 Oct;75(19):6306-11
references.pmid: 19684168
references.citation: Appl Environ Microbiol. 2009 Feb;75(4):981-90
references.pmid: 19114526
references.citation: Bioresour Technol. 2003 Sep;89(2):125-31
references.pmid: 12699930
references.citation: J Bacteriol. 1993 Jun;175(12):3863-75
references.pmid: 7685336
references.citation: J Bacteriol. 2000 Jun;182(11):3072-80
references.pmid: 10809684
references.citation: J Gen Microbiol. 1991 Jul;137(7):1715-20
references.pmid: 1683400
references.citation: Appl Environ Microbiol. 1990 Sep;56(9):2644-9
references.pmid: 16348274
references.citation: Biotechnol Lett. 2012 Oct;34(10):1877-85
references.pmid: 22714279
references.citation: Plasmid. 2005 Nov;54(3):241-8
references.pmid: 16005967
references.citation: J Biol Chem. 1945 Dec;161:495-502
references.pmid: 21006932
references.citation: PLoS One. 2011;6(5):e20092
references.pmid: 21625606
references.citation: Crit Rev Microbiol. 2007 Apr-Jun;33(2):127-40
references.pmid: 17558661
references.citation: J Bacteriol. 1999 Jun;181(12):3837-41
references.pmid: 10368162
references.citation: Arch Microbiol. 2004 Dec;182(6):467-74
references.pmid: 15365694