title: Granulocytes of the red claw crayfish Cherax quadricarinatus can endocytose beads, E. coli and WSSV, but in different ways.
abstract: The hemocytes of the red claw crayfish Cherax quadricarinatus are classified by morphologic observation into the following types: hyalinocytes (H), semi-granulocytes (SG) and granulocytes (G). Density gradient centrifugation with Percoll was developed to separate these three subpopulations of hemocytes. Beads, Escherichia coli, and FITC labeling WSSV were used to investigate the characteristics of granulocytes by using scanning electron microscope, transmission electron microscope, and laser scan confocal microscope. Results showed that granulocytes could phagocytose beads and E. coli by endocytic pathways. WSSV could rely on caveolae-mediated endocytosis to mainly enter into granulocytes. These results could elucidate the mechanism of the innate immunity function of granulocytes, and it also showed the mechanism by which WSSV invaded granulocytes in the red claw crayfish.
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Rd., Qingdao 266071, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Hu
authors.initials: H
authors.lastname: Duan
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Rd., Qingdao 266071, China.
authors.forename: Songjun
authors.initials: S
authors.lastname: Jin
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Rd., Qingdao 266071, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Rd., Qingdao 266071, China.
authors.forename: Fuhua
authors.initials: F
authors.lastname: Li
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Rd., Qingdao 266071, China. Electronic address: jhxiang@qdio.ac.cn.
authors.forename: Jianhai
authors.initials: J
authors.lastname: Xiang
mesh_terms: D000818:Animals; D003400:Astacoidea / Q000166:cytology* / Q000276:immunology; D002478:Cells, Cultured; D004926:Escherichia coli / Q000502:physiology*; D005456:Fluorescent Dyes / Q000378:metabolism; D006098:Granulocytes / Q000502:physiology* / Q000648:ultrastructure / Q000821:virology; D006434:Hemocytes / Q000502:physiology / Q000648:ultrastructure; D054884:Host-Pathogen Interactions; D007113:Immunity, Innate; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D010587:Phagocytosis; D053586:Virus Internalization; D046848:White spot syndrome virus 1 / Q000502:physiology*
chemical_list: D005456:Fluorescent Dyes
keywords: Beads; Cherax quadricarinatus; E. coli; Endocytosis; Granulocyte; WSSV