title: S100A6 and its extracellular targets in Wharton's jelly of healthy and preeclamptic patients.
abstract: BACKGROUND
  In this work we compared the level, localization and binding partners of a calcium binding protein, S100A6, in extracellular matrix of Wharton's jelly of healthy and preeclamptic patients.
  
  
  METHODS
  Studies were performed on the umbilical cords taken from 10 newborns delivered by healthy and 10 newborns delivered by preeclamptic mothers. To characterize S100A6 in Wharton's jelly immunoblotting and immunohistochemistry were applied. For identification of S100A6 targets pull down assays and mass spectrometry were performed. Direct interaction of S100A6 with its targets was checked by ELISA while co-localization of these proteins was analyzed by immunofluorescence staining.
  
  
  RESULTS
  We have found that the level of S100A6 in Wharton's jelly is higher in patients with preeclampsia than in healthy ones and that post-translational modifications of S100A6 in preeclamptic tissue are different than those of S100A6 in control. We have identified several proteins that might interact with S100A6, among them are lumican and PRELP, found in Wharton's jelly of healthy and preeclamptic patients, and IGFBP-1 identified, as an S100A6 target, only in preeclamptic tissue. We have shown that the interactions between S100A6 and these proteins are direct and that IGF-1 competes with S100A6 for binding to IGFBP-1.
  
  
  CONCLUSIONS
  In Wharton's jelly of preeclamptic tissue S100A6 is up-regulated and binds to different targets than in control. This suggests involvement of S100A6 in development of preeclampsia.
authors.affiliation: Nencki Institute of Experimental Biology, 3 Pasteur Street, 02-093 Warsaw, Poland.
authors.forename: E
authors.initials: E
authors.lastname: Jurewicz
authors.affiliation: Medical University of Bialystok, 1 Kilinski Street, 15-089 Bialystok, Poland.
authors.forename: I
authors.initials: I
authors.lastname: Kasacka
authors.affiliation: Medical University of Bialystok, 1 Kilinski Street, 15-089 Bialystok, Poland.
authors.forename: E
authors.initials: E
authors.lastname: Bankowski
authors.affiliation: Nencki Institute of Experimental Biology, 3 Pasteur Street, 02-093 Warsaw, Poland. Electronic address: a.filipek@nencki.gov.pl.
authors.forename: A
authors.initials: A
authors.lastname: Filipek
mesh_terms: D000328:Adult; D018797:Cell Cycle Proteins / Q000032:analysis* / Q000378:metabolism; D011508:Chondroitin Sulfate Proteoglycans / Q000378:metabolism; D005109:Extracellular Matrix / Q000737:chemistry*; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D005260:Female; D005455:Fluorescent Antibody Technique; D005865:Gestational Age; D006023:Glycoproteins / Q000378:metabolism; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D007231:Infant, Newborn; D018970:Insulin-Like Growth Factor Binding Protein 1 / Q000378:metabolism; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology; D007632:Keratan Sulfate / Q000378:metabolism; D000071234:Lumican; D011225:Pre-Eclampsia / Q000378:metabolism*; D011247:Pregnancy; D011499:Protein Processing, Post-Translational; D000075383:S100 Calcium Binding Protein A6; D009418:S100 Proteins / Q000032:analysis* / Q000378:metabolism; D059631:Wharton Jelly / Q000737:chemistry*
chemical_list: D018797:Cell Cycle Proteins; D011508:Chondroitin Sulfate Proteoglycans; D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; D018970:Insulin-Like Growth Factor Binding Protein 1; C000604306:LUM protein, human; D000071234:Lumican; C096382:PRELP protein, human; D000075383:S100 Calcium Binding Protein A6; D009418:S100 Proteins; C055351:S100A6 protein, human; D007334:Insulin-Like Growth Factor I; D007632:Keratan Sulfate
keywords: Preeclampsia; S100A6; Target proteins; Umbilical cord; Wharton's jelly