title: Composition and cytotoxicity of a novel polysaccharide from brown alga (Laminaria japonica).
abstract: A novel polysaccharide WPS-2-1, with an average molecular weight of 80 kDa, was purified from aqueous extracts of Laminaria japonica. Monosaccharides analysis revealed that WPS-2-1 was composed of mannose, rhamnose and fucose with a molar ratio of 1.0:2.3:1.2. Analysis by periodate oxidation-Smith degradation indicated that WPS-2-1 had a backbone of array by (1→4)-glycosidic linkages. Cytotoxicity assay showed that WPS-2-1 presented significantly higher antitumor activities against A375 and BGC823 cells with a dose-dependent manner, and exhibited lower cytotoxicity to vascular smooth muscle cells. The results suggested that WPS-2-1 should be explored as a potential antitumor agent with low toxicity.
authors.affiliation: Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou, China; College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou, China.
authors.forename: Zhenfei
authors.initials: Z
authors.lastname: Peng
authors.affiliation: Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou, China; College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou, China.
authors.forename: Min
authors.initials: M
authors.lastname: Liu
authors.affiliation: Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou, China.
authors.forename: Zhexiang
authors.initials: Z
authors.lastname: Fang
authors.affiliation: Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou, China.
authors.forename: Jiulin
authors.initials: J
authors.lastname: Wu
authors.affiliation: Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou, China; Institute of Biomedical Engineering, Chinese Academy of Medical Science and Peking Union Medical College, Tianjin, China.
authors.forename: Qiqing
authors.initials: Q
authors.lastname: Zhang
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D002236:Carbohydrate Conformation; D045744:Cell Line, Tumor; D003603:Cytotoxins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D007795:Laminaria / Q000737:chemistry*; D009131:Muscle, Smooth, Vascular / Q000378:metabolism / Q000473:pathology; D032389:Myocytes, Smooth Muscle / Q000378:metabolism / Q000473:pathology; D009369:Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D000970:Antineoplastic Agents; D003603:Cytotoxins; D011134:Polysaccharides