title: Diversity and transport of microorganisms in intertidal sands of the California coast.
abstract: Forced by tides and waves, large volumes of seawater are flushed through the beach daily. Organic material and nutrients in seawater are remineralized and cycled as they pass through the beach. Microorganisms are responsible for most of the biogeochemical cycling in the beach; however, few studies have characterized their diversity in intertidal sands, and little work has characterized the extent to which microbes are transported between different compartments of the beach. The present study uses next-generation massively parallel sequencing to characterize the microbial community present at 49 beaches along the coast of California. In addition, we characterize the transport of microorganisms within intertidal sands using laboratory column experiments. We identified extensive diversity in the beach sands. Nearly 1,000 unique taxa were identified in sands from 10 or more unique beaches, suggesting the existence of a group of "cosmopolitan" sand microorganisms. A biogeographical analysis identified a taxon-distance relationship among the beaches. In addition, sands with similar grain size, organic carbon content, exposed to a similar wave climate, and having the same degree of anthropogenic influence tended to have similar microbial communities. Column experiments identified microbes readily mobilized by seawater infiltrating through unsaturated intertidal sands. The ease with which microbes were mobilized suggests that intertidal sands may represent a reservoir of bacteria that seed the beach aquifer where they may partake in biogeochemical cycling.
authors.affiliation: Environmental and Water Studies, Department of Civil and Environmental Engineering, Stanford University, Stanford, California, USA aboehm@stanford.edu.
authors.forename: Alexandria B
authors.initials: AB
authors.lastname: Boehm
authors.affiliation: Environmental and Water Studies, Department of Civil and Environmental Engineering, Stanford University, Stanford, California, USA.
authors.forename: Kevan M
authors.initials: KM
authors.lastname: Yamahara
authors.affiliation: Environmental and Water Studies, Department of Civil and Environmental Engineering, Stanford University, Stanford, California, USA.
authors.forename: Lauren M
authors.initials: LM
authors.lastname: Sassoubre
mesh_terms: D058448:Biota; D002140:California; D059014:High-Throughput Nucleotide Sequencing; D008969:Molecular Sequence Data; D058974:Phylogeography; D012988:Soil Microbiology
references.citation: Proc Natl Acad Sci U S A. 2010 May 11;107(19):8818-23
references.pmid: 20421470
references.citation: Ann Rev Mar Sci. 2014;6:23-51
references.pmid: 23987916
references.citation: Appl Environ Microbiol. 2012 Mar;78(6):1733-45
references.pmid: 22247142
references.citation: ISME J. 2012 Dec;6(12):2229-44
references.pmid: 22717884
references.citation: BMC Bioinformatics. 2003 Apr 3;4:12
references.pmid: 12697067
references.citation: PLoS One. 2013 Sep 10;8(9):e74265
references.pmid: 24040219
references.citation: Nature. 2003 Apr 10;422(6932):608-11
references.pmid: 12686999
references.citation: Environ Microbiol. 2006 Dec;8(12):2201-13
references.pmid: 17107561
references.citation: ISME J. 2009 Nov;3(11):1314-7
references.pmid: 19587772
references.citation: PLoS Genet. 2008 Nov;4(11):e1000255
references.pmid: 19023400
references.citation: Appl Environ Microbiol. 2013 Jun;79(12):3601-9
references.pmid: 23563940
references.citation: PLoS One. 2012;7(9):e44224
references.pmid: 22970184
references.citation: J Microbiol Methods. 2010 Nov;83(2):141-8
references.pmid: 20801169
references.citation: Environ Sci Technol. 2012 Jun 5;46(11):5988-96
references.pmid: 22533299
references.citation: J Ind Microbiol Biotechnol. 2009 Jul;36(7):981-8
references.pmid: 19408027
references.citation: Int Microbiol. 2010 Jun;13(2):59-65
references.pmid: 20890840
references.citation: Appl Environ Microbiol. 2011 Nov;77(22):7962-74
references.pmid: 21948834
references.citation: PLoS One. 2014 Mar 05;9(3):e90815
references.pmid: 24599478
references.citation: Arch Microbiol. 2003 Nov;180(5):327-38
references.pmid: 14504692
references.citation: Appl Environ Microbiol. 2007 Aug;73(16):5261-7
references.pmid: 17586664
references.citation: Syst Appl Microbiol. 2003 Mar;26(1):47-53
references.pmid: 12747409
references.citation: Appl Environ Microbiol. 2006 Sep;72(9):5689-701
references.pmid: 16957183
references.citation: Microbiol Rev. 1982 Mar;46(1):43-70
references.pmid: 7045624
references.citation: PLoS One. 2011;6(11):e28132
references.pmid: 22132229
references.citation: Appl Environ Microbiol. 2008 Jul;74(14):4440-53
references.pmid: 18487394
references.citation: Syst Appl Microbiol. 2006 Jun;29(4):333-48
references.pmid: 16431068
references.citation: Environ Microbiol. 2008 Apr;10(4):1068-79
references.pmid: 18266758
references.citation: PLoS One. 2011;6(6):e20956
references.pmid: 21738596
references.citation: Environ Microbiol. 2000 Oct;2(5):542-54
references.pmid: 11233162
references.citation: Genome Biol. 2010;11(10):R106
references.pmid: 20979621
references.citation: Appl Environ Microbiol. 2006 Mar;72(3):2102-9
references.pmid: 16517659
references.citation: Genome Biol. 2004;5(10):R80
references.pmid: 15461798
references.citation: Bioinformatics. 2010 Jan 1;26(1):139-40
references.pmid: 19910308
references.citation: Nat Methods. 2013 Dec;10(12):1200-2
references.pmid: 24076764
references.citation: PLoS One. 2009 Oct 09;4(10):e7401
references.pmid: 19816594
references.citation: ISME J. 2009 Jul;3(7):780-91
references.pmid: 19340087
references.citation: BMC Genomics. 2009 Dec 17;10:616
references.pmid: 20017926
references.citation: ISME J. 2012 Mar;6(3):542-53
references.pmid: 21975598
references.citation: Nat Rev Microbiol. 2012 May 14;10(7):497-506
references.pmid: 22580365
references.citation: Environ Microbiol. 2011 May;13(5):1138-52
references.pmid: 21176054
references.citation: FEMS Microbiol Ecol. 2003 May 1;44(1):89-100
references.pmid: 19719654
references.citation: Nat Methods. 2009 Sep;6(9):639-41
references.pmid: 19668203
references.citation: Nat Methods. 2010 May;7(5):335-6
references.pmid: 20383131
references.citation: Environ Sci Technol. 2014 Apr 1;48(7):4038-47
references.pmid: 24625288