title: Roles of interleukin-9 in the growth and cholecystokinin-induced intracellular calcium signaling of cultured interstitial cells of Cajal.
abstract: Interstitial cells of Cajal (ICC) are pacemaker cells in the gastrointestinal (GI) tract and loss of ICC is associated with many GI motility disorders. Previous studies have shown that ICC have the capacity to regenerate or restore, and several growth factors are critical to their growth, maintenance or regeneration. The present study aimed to investigate the roles of interleukin-9 (IL-9) in the growth, maintenance and pacemaker functions of cultured ICC. Here, we report that IL-9 promotes proliferation of ICC, and culturing ICC with IL-9 enhances cholecystokinin-8-induced Ca²⁺ transients, which is probably caused by facilitating maintenance of ICC functions under culture condition. We also show co-localizations of cholecystokinin-1 receptor and IL-9 receptor with c-kit by double-immunohistochemical labeling. In conclusion, IL-9 can promote ICC growth and help maintain ICC functions; IL-9 probably performs its functions via IL-9 receptors on ICC.
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Yaoyao
authors.initials: Y
authors.lastname: Gong
authors.affiliation: Department of Pediatric Surgery, Nanjing Children's Hospital Affiliated to Nanjing Medical University, Nanjing, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Huang
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Wenfang
authors.initials: W
authors.lastname: Cheng
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Xueliang
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Lu
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Lin
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Xinmin
authors.initials: X
authors.lastname: Si
mesh_terms: D000818:Animals; D000075369:Anoctamin-1; D020013:Calcium Signaling; D049109:Cell Proliferation; D002478:Cells, Cultured; D018118:Chloride Channels / Q000378:metabolism; D002766:Cholecystokinin / Q000502:physiology*; D005260:Female; D016906:Interleukin-9 / Q000502:physiology*; D056885:Interstitial Cells of Cajal / Q000502:physiology*; D008297:Male; D008807:Mice, Inbred BALB C; D011706:Pyloric Antrum / Q000166:cytology; D053651:Receptors, Interleukin-9 / Q000378:metabolism; D012844:Sincalide / Q000502:physiology
chemical_list: C531181:ANO1 protein, mouse; D000075369:Anoctamin-1; D018118:Chloride Channels; D016906:Interleukin-9; D053651:Receptors, Interleukin-9; D002766:Cholecystokinin; D012844:Sincalide
references.citation: J Membr Biol. 2010 Apr;234(3):217-25
references.pmid: 20349180
references.citation: J Physiol. 2010 Nov 15;588(Pt 22):4453-74
references.pmid: 20876203
references.citation: Virchows Arch. 2006 Sep;449(3):348-57
references.pmid: 16912883
references.citation: J Leukoc Biol. 1995 Mar;57(3):353-60
references.pmid: 7884304
references.citation: J Immunol. 2004 Aug 15;173(4):2771-9
references.pmid: 15294996
references.citation: J Physiol. 2003 Jan 15;546(Pt 2):415-25
references.pmid: 12527728
references.citation: J Physiol. 2001 Oct 15;536(Pt 2):555-68
references.pmid: 11600689
references.citation: Mol Cell Biol. 1996 Sep;16(9):4710-6
references.pmid: 8756628
references.citation: J Immunol. 2003 Apr 1;170(7):3461-7
references.pmid: 12646606
references.citation: Gastroenterology. 2008 Apr;134(4):1083-93
references.pmid: 18395089
references.citation: J Physiol. 2001 Mar 15;531(Pt 3):827-33
references.pmid: 11251061
references.citation: J Physiol. 2010 Aug 15;588(Pt 16):3101-17
references.pmid: 20581042
references.citation: Annu Rev Physiol. 2006;68:307-43
references.pmid: 16460275
references.citation: Gastroenterology. 2006 Mar;130(3):759-70
references.pmid: 16530517
references.citation: Am J Physiol Cell Physiol. 2004 Feb;286(2):C448-56
references.pmid: 14534083
references.citation: J Biol Regul Homeost Agents. 2012 Jul-Sep;26(3):319-26
references.pmid: 23034251
references.citation: J Cell Sci. 2004 Jun 1;117(Pt 13):2813-25
references.pmid: 15169838
references.citation: J Immunol. 2001 Feb 15;166(4):2768-74
references.pmid: 11160343
references.citation: J Physiol. 2003 Dec 1;553(Pt 2):545-59
references.pmid: 14500772
references.citation: J Cell Mol Med. 2012 Feb;16(2):318-27
references.pmid: 21426484
references.citation: Am J Respir Crit Care Med. 2011 Apr 1;183(7):865-75
references.pmid: 20971830
references.citation: Am J Gastroenterol. 2002 Jan;97(1):118-25
references.pmid: 11808934
references.citation: Neurogastroenterol Motil. 2007 May;19(5):349-64
references.pmid: 17509017
references.citation: Diabetes. 2005 May;54(5):1528-33
references.pmid: 15855342
references.citation: J Cell Mol Med. 2006 Jul-Sep;10(3):687-94
references.pmid: 16989728
references.citation: Neurogastroenterol Motil. 2012 May;24(5):e221-34
references.pmid: 22292914
references.citation: J Physiol. 2008 Nov 1;586(21):5047-61
references.pmid: 18787034
references.citation: World J Gastroenterol. 2012 Dec 28;18(48):7184-93
references.pmid: 23326123
references.citation: PLoS One. 2011;6(9):e24928
references.pmid: 21949791
references.citation: Am J Physiol Gastrointest Liver Physiol. 2011 Nov;301(5):G835-45
references.pmid: 21836058
references.citation: J Immunol. 2011 Mar 15;186(6):3283-8
references.pmid: 21368237
references.citation: Exp Physiol. 2008 Mar;93(3):370-82
references.pmid: 18156167