title: Synthesis and self-assembly of amphiphilic aptamer-functionalized hyperbranched multiarm copolymers for targeted cancer imaging.
abstract: A novel targeting cancer imaging platform based on aptamer-functionalized amphiphilic hyperbranched copolymer conjugates, which can self-assemble into nanoscopic micelles with a core-shell structure and a narrow size distribution, has been designed and synthesized. The size, morphology, fluorescence performance, and cytotoxicity of micelles were studied by dynamic light scattering, transmission electron microscopy, fluorescence spectroscopy, and a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide colorimetric assay. The results indicate that these micelles have low cytotoxicity against MCF-7 cells and can be easily internalized by MCF-7 cells. In addition, they also exhibit enhanced cell uptake, excellent fluorescence properties, and smart targeting capability in vitro, indicating great potential to be promising carriers for bioimaging and cancer specific delivery.
authors.affiliation: School of Chemistry and Chemical Engineering, State Key Laboratory of Metal Matrix Composites, Shanghai Jiao Tong University , 800 Dongchuan Road, Shanghai 200240, People's Republic of China.
authors.forename: Songrui
authors.initials: S
authors.lastname: Yu
authors.forename: Ruijiao
authors.initials: R
authors.lastname: Dong
authors.forename: Jianxin
authors.initials: J
authors.lastname: Chen
authors.forename: Feng
authors.initials: F
authors.lastname: Chen
authors.forename: Wenfeng
authors.initials: W
authors.lastname: Jiang
authors.forename: Yongfeng
authors.initials: Y
authors.lastname: Zhou
authors.forename: Xinyuan
authors.initials: X
authors.lastname: Zhu
authors.forename: Deyue
authors.initials: D
authors.lastname: Yan
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004705:Endocytosis; D006801:Humans; D061986:MCF-7 Cells; D051379:Mice; D057054:Molecular Imaging / Q000379:methods*; D015394:Molecular Structure; D041681:NIH 3T3 Cells; D053758:Nanoparticles / Q000737:chemistry / Q000633:toxicity; D010316:Particle Size; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D013499:Surface Properties; D013501:Surface-Active Agents / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology
chemical_list: D052157:Aptamers, Nucleotide; D011108:Polymers; D013501:Surface-Active Agents