title: HLA-G expression on blasts and tolerogenic cells in patients affected by acute myeloid leukemia.
abstract: Human Leukocyte Antigen-G (HLA-G) contributes to cancer cell immune escape from host antitumor responses. The clinical relevance of HLA-G in several malignancies has been reported. However, the role of HLA-G expression and functions in Acute Myeloid Leukemia (AML) is still controversial. Our group identified a subset of tolerogenic dendritic cells, DC-10 that express HLA-G and secrete IL-10. DC-10 are present in the peripheral blood and are essential in promoting and maintaining tolerance via the induction of adaptive T regulatory (Treg) cells. We investigated HLA-G expression on blasts and the presence of HLA-G-expressing DC-10 and CD4(+) T cells in the peripheral blood of AML patients at diagnosis. Moreover, we explored the possible influence of the 3' untranslated region (3'UTR) of HLA-G, which has been associated with HLA-G expression, on AML susceptibility. Results showed that HLA-G-expressing DC-10 and CD4(+) T cells are highly represented in AML patients with HLA-G positive blasts. None of the HLA-G variation sites evaluated was associated with AML susceptibility. This is the first report describing HLA-G-expressing DC-10 and CD4(+) T cells in AML patients, suggesting that they may represent a strategy by which leukemic cells escape the host's immune system. Further studies on larger populations are required to verify our findings.
authors.affiliation: San Raffaele Telethon Institute for Gene Therapy (HSR-TIGET), Division of Regenerative Medicine, Stem Cells and Gene Therapy, San Raffaele Scientific Institute (HSR-DREaM-GENE), Via Olgettina 58, 20132 Milan, Italy ; Ph.D Program in Translational and Molecular Medicine (DIMET), University of Milan-Bicocca, Piazza della Scienza 3, 20126 Milan, Italy.
authors.forename: Grazia
authors.identifier: 0000-0002-6456-1320
authors.initials: G
authors.lastname: Locafaro
authors.affiliation: San Raffaele Telethon Institute for Gene Therapy (HSR-TIGET), Division of Regenerative Medicine, Stem Cells and Gene Therapy, San Raffaele Scientific Institute (HSR-DREaM-GENE), Via Olgettina 58, 20132 Milan, Italy.
authors.forename: Giada
authors.identifier: 0000-0002-2457-387X
authors.initials: G
authors.lastname: Amodio
authors.affiliation: San Raffaele Telethon Institute for Gene Therapy (HSR-TIGET), Division of Regenerative Medicine, Stem Cells and Gene Therapy, San Raffaele Scientific Institute (HSR-DREaM-GENE), Via Olgettina 58, 20132 Milan, Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: Tomasoni
authors.affiliation: Immunohematology Blood Transfusion Service (S.I.M.T.), San Raffaele Hospital, Via Olgettina 60, 20132 Milan, Italy.
authors.forename: Cristina
authors.identifier: 0000-0001-7711-5621
authors.initials: C
authors.lastname: Tresoldi
authors.affiliation: Hematology and Bone Marrow Transplantation Unit, Division of Regenerative Medicine, Stem Cells and Gene Therapy, San Raffaele Scientific Institute (HSR-DREaM-GENE), Via Olgettina 58, 20132 Milan, Italy.
authors.forename: Fabio
authors.initials: F
authors.lastname: Ciceri
authors.affiliation: San Raffaele Telethon Institute for Gene Therapy (HSR-TIGET), Division of Regenerative Medicine, Stem Cells and Gene Therapy, San Raffaele Scientific Institute (HSR-DREaM-GENE), Via Olgettina 58, 20132 Milan, Italy.
authors.forename: Silvia
authors.identifier: 0000-0002-3517-9683
authors.initials: S
authors.lastname: Gregori
mesh_terms: D020413:3' Untranslated Regions; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D003713:Dendritic Cells / Q000276:immunology / Q000473:pathology*; D004198:Disease Susceptibility; D005260:Female; D015870:Gene Expression / Q000276:immunology*; D059951:HLA-G Antigens / Q000235:genetics* / Q000276:immunology; D006801:Humans; D057131:Immune Evasion; D007108:Immune Tolerance; D016753:Interleukin-10 / Q000276:immunology / Q000378:metabolism; D015470:Leukemia, Myeloid, Acute / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D008297:Male; D008875:Middle Aged; D050378:T-Lymphocytes, Regulatory / Q000276:immunology / Q000473:pathology
chemical_list: D020413:3' Untranslated Regions; D059951:HLA-G Antigens; C508609:IL10 protein, human; D016753:Interleukin-10
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