title: Verrucomicrobia are candidates for polysaccharide-degrading bacterioplankton in an arctic fjord of Svalbard.
abstract: In Arctic marine bacterial communities, members of the phylum Verrucomicrobia are consistently detected, although not typically abundant, in 16S rRNA gene clone libraries and pyrotag surveys of the marine water column and in sediments. In an Arctic fjord (Smeerenburgfjord) of Svalbard, members of the Verrucomicrobia, together with Flavobacteria and smaller proportions of Alpha- and Gammaproteobacteria, constituted the most frequently detected bacterioplankton community members in 16S rRNA gene-based clone library analyses of the water column. Parallel measurements in the water column of the activities of six endo-acting polysaccharide hydrolases showed that chondroitin sulfate, laminarin, and xylan hydrolysis accounted for most of the activity. Several Verrucomicrobia water column phylotypes were affiliated with previously sequenced, glycoside hydrolase-rich genomes of individual Verrucomicrobia cells that bound fluorescently labeled laminarin and xylan and therefore constituted candidates for laminarin and xylan hydrolysis. In sediments, the bacterial community was dominated by different lineages of Verrucomicrobia, Bacteroidetes, and Proteobacteria but also included members of multiple phylum-level lineages not observed in the water column. This community hydrolyzed laminarin, xylan, chondroitin sulfate, and three additional polysaccharide substrates at high rates. Comparisons with data from the same fjord in the previous summer showed that the bacterial community in Smeerenburgfjord changed in composition, most conspicuously in the changing detection frequency of Verrucomicrobia in the water column. Nonetheless, in both years the community hydrolyzed the same polysaccharide substrates.
authors.affiliation: Department of Marine Sciences, University of North Carolina-Chapel Hill, Chapel Hill, North Carolina, USA.
authors.forename: Z
authors.initials: Z
authors.lastname: Cardman
authors.affiliation: Department of Marine Sciences, University of North Carolina-Chapel Hill, Chapel Hill, North Carolina, USA.
authors.forename: C
authors.initials: C
authors.lastname: Arnosti
authors.affiliation: Department of Marine Sciences, University of North Carolina-Chapel Hill, Chapel Hill, North Carolina, USA.
authors.forename: A
authors.initials: A
authors.lastname: Durbin
authors.affiliation: Department of Marine Sciences, University of North Carolina-Chapel Hill, Chapel Hill, North Carolina, USA.
authors.forename: K
authors.initials: K
authors.lastname: Ziervogel
authors.affiliation: Department of Marine Sciences, University of North Carolina-Chapel Hill, Chapel Hill, North Carolina, USA.
authors.forename: C
authors.initials: C
authors.lastname: Cox
authors.affiliation: Department of Marine Sciences, University of North Carolina-Chapel Hill, Chapel Hill, North Carolina, USA.
authors.forename: A D
authors.initials: AD
authors.lastname: Steen
authors.affiliation: Department of Marine Sciences, University of North Carolina-Chapel Hill, Chapel Hill, North Carolina, USA teske@email.unc.edu.
authors.forename: A
authors.initials: A
authors.lastname: Teske
mesh_terms: D001110:Arctic Regions; D008969:Molecular Sequence Data; D010802:Phylogeny; D011134:Polysaccharides / Q000378:metabolism*; D012623:Seawater / Q000382:microbiology*; D013538:Svalbard; D061327:Verrucomicrobia / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*
chemical_list: D011134:Polysaccharides
references.citation: Mar Genomics. 2013 Mar;9:51-61
references.pmid: 23273849
references.citation: mBio. 2013 May 28;4(3):e00569-12
references.pmid: 23716574
references.citation: Proc Natl Acad Sci U S A. 2011 Aug 23;108(34):14288-93
references.pmid: 21825123
references.citation: Appl Environ Microbiol. 2005 Dec;71(12):8402-10
references.pmid: 16332828
references.citation: Ambio. 2004 Dec;33(8):565-75
references.pmid: 15666690
references.citation: FEMS Microbiol Ecol. 2007 Mar;59(3):671-82
references.pmid: 17381520
references.citation: FEMS Microbiol Ecol. 2005 Aug 1;53(3):379-91
references.pmid: 16329957
references.citation: Nature. 2007 Dec 6;450(7171):874-8
references.pmid: 18004305
references.citation: Appl Environ Microbiol. 2002 Feb;68(2):505-18
references.pmid: 11823184
references.citation: Science. 2011 Sep 2;333(6047):1296-300
references.pmid: 21885783
references.citation: Appl Environ Microbiol. 2009 Mar;75(6):1589-96
references.pmid: 19151179
references.citation: Appl Environ Microbiol. 1999 Sep;65(9):3982-9
references.pmid: 10473405
references.citation: Front Microbiol. 2013 Oct 31;4:318
references.pmid: 24198812
references.citation: Proc Natl Acad Sci U S A. 2012 Oct 23;109(43):17633-8
references.pmid: 23045668
references.citation: Appl Environ Microbiol. 2000 Apr;66(4):1741-3
references.pmid: 10742274
references.citation: Appl Environ Microbiol. 2005 Mar;71(3):1501-6
references.pmid: 15746353
references.citation: Int J Syst Evol Microbiol. 2001 Nov;51(Pt 6):1965-1968
references.pmid: 11760935
references.citation: Int J Syst Evol Microbiol. 2007 May;57(Pt 5):959-963
references.pmid: 17473241
references.citation: Arch Microbiol. 2008 Jun;189(6):531-9
references.pmid: 18253713
references.citation: Antonie Van Leeuwenhoek. 2013 Jan;103(1):99-119
references.pmid: 22903492
references.citation: Science. 1992 Mar 20;255(5051):1561-4
references.pmid: 17820170
references.citation: Bioinformatics. 2004 Sep 22;20(14):2317-9
references.pmid: 15073015
references.citation: Int J Syst Evol Microbiol. 2008 Apr;58(Pt 4):998-1007
references.pmid: 18398209
references.citation: Appl Environ Microbiol. 2008 Jan;74(2):503-15
references.pmid: 18039821
references.citation: Appl Environ Microbiol. 2002 Apr;68(4):1994-2007
references.pmid: 11916723
references.citation: Mol Biol Evol. 1987 Jul;4(4):406-25
references.pmid: 3447015
references.citation: Microbiol Mol Biol Rev. 2005 Sep;69(3):440-61
references.pmid: 16148306
references.citation: Proc Natl Acad Sci U S A. 2009 Dec 29;106(52):22427-32
references.pmid: 20018741
references.citation: FEMS Microbiol Ecol. 2007 Jan;59(1):108-17
references.pmid: 17233748
references.citation: Front Microbiol. 2013 May 09;4:112
references.pmid: 23675372
references.citation: FEMS Microbiol Lett. 1998 Jan 1;158(1):9-15
references.pmid: 9453151
references.citation: ISME J. 2010 Apr;4(4):564-76
references.pmid: 20010630
references.citation: Appl Environ Microbiol. 2000 Dec;66(12):5116-22
references.pmid: 11097877
references.citation: Nature. 2007 Dec 6;450(7171):879-82
references.pmid: 18004300
references.citation: FEMS Microbiol Ecol. 2005 Jan 1;51(2):265-77
references.pmid: 16329875
references.citation: Nucleic Acids Res. 2007;35(21):7188-96
references.pmid: 17947321
references.citation: J Chromatogr B Analyt Technol Biomed Life Sci. 2003 Aug 5;793(1):181-91
references.pmid: 12880865
references.citation: Environ Microbiol. 2012 Jan;14(1):52-66
references.pmid: 21895912
references.citation: Curr Microbiol. 2014 Feb;68(2):233-8
references.pmid: 24121613
references.citation: Ann Rev Mar Sci. 2011;3:401-25
references.pmid: 21329211
references.citation: Nat Rev Microbiol. 2008 Jun;6(6):441-54
references.pmid: 18461075
references.citation: PLoS One. 2012;7(4):e35314
references.pmid: 22536372
references.citation: Proc Natl Acad Sci U S A. 2003 Jul 8;100(14):8298-303
references.pmid: 12835416
references.citation: Antonie Van Leeuwenhoek. 1997 Jul;72(1):29-38
references.pmid: 9296261
references.citation: Appl Environ Microbiol. 2011 Mar;77(6):2008-18
references.pmid: 21257812
references.citation: Int J Syst Evol Microbiol. 2008 Nov;58(Pt 11):2491-500
references.pmid: 18984682
references.citation: Proc Natl Acad Sci U S A. 2008 Jan 8;105(1):300-4
references.pmid: 18172218
references.citation: Proc Natl Acad Sci U S A. 2011 Aug 2;108(31):12776-81
references.pmid: 21768380
references.citation: Proc Natl Acad Sci U S A. 2000 Feb 15;97(4):1813-7
references.pmid: 10660683
references.citation: Annu Rev Microbiol. 2006;60:255-80
references.pmid: 16719716
references.citation: Appl Environ Microbiol. 2000 Oct;66(10):4361-5
references.pmid: 11010883
references.citation: FEMS Microbiol Ecol. 2002 Feb 1;39(2):91-100
references.pmid: 19709188
references.citation: Appl Environ Microbiol. 2004 Feb;70(2):781-9
references.pmid: 14766555
references.citation: J Bacteriol. 1998 Sep;180(18):4765-74
references.pmid: 9733676
references.citation: Nat Rev Microbiol. 2011 Jun;9(6):403-13
references.pmid: 21572457
references.citation: Nat Rev Microbiol. 2007 Oct;5(10):782-91
references.pmid: 17853906
references.citation: PLoS Genet. 2008 May 30;4(5):e1000087
references.pmid: 18516288
references.citation: PLoS One. 2011;6(12):e28900
references.pmid: 22216139
references.citation: Science. 2012 May 4;336(6081):608-11
references.pmid: 22556258
references.citation: Proc Natl Acad Sci U S A. 2012 Jul 3;109(27):E1878-87
references.pmid: 22699508
references.citation: FEMS Microbiol Ecol. 2008 Nov;66(2):343-51
references.pmid: 18778275
references.citation: Int J Syst Bacteriol. 1996 Oct;46(4):960-6
references.pmid: 8863424
references.citation: Microb Ecol. 2011 Oct;62(3):537-48
references.pmid: 21556884
references.citation: Appl Environ Microbiol. 2004 Oct;70(10):5875-81
references.pmid: 15466527
references.citation: Brief Bioinform. 2008 Jul;9(4):299-306
references.pmid: 18417537
references.citation: ISME J. 2014 Nov;8(11):2167-79
references.pmid: 24763371
references.citation: Environ Microbiol. 2006 Dec;8(12):2201-13
references.pmid: 17107561
references.citation: Appl Environ Microbiol. 2003 May;69(5):2463-83
references.pmid: 12732511