title: Ultrasensitive detection of human liver hepatocellular carcinoma cells using a label-free aptasensor.
abstract: Liver cancer is one of the most common cancers in the world and has no effective cure, especially in later stages. The development of a tangible protocol for early diagnosis of this disease remains a major challenge. In the present manuscript, an aptamer-based, label-free electrochemical biosensor for the sensitive detection of HepG2, a hepatocellular carcinoma cell line, is described. The target cells are captured in a sandwich architecture using TLS11a aptamer covalently attached to a gold surface and a secondary TLS11a aptamer. The application of TLS11a aptamer as a recognition layer resulted in a sensor with high affinity for HepG2 cancer cells in comparison with control cancer cells of human prostate, breast, and colon tumors. The aptasensor delivered a wide linear dynamic range over 1 × 10(2) to 1 × 10(6) cells/mL, with a detection limit of 2 cells/mL. This protocol provides a precise method for sensitive detection of liver cancer with significant advantages in terms of simplicity, low cost, and stability.
authors.affiliation: Biosensors and Bioelectronics Centre, Institute of Physics, Chemistry and Biology, Linköping University , Linköping S-58183, Sweden.
authors.forename: Leila
authors.initials: L
authors.lastname: Kashefi-Kheyrabadi
authors.forename: Masoud A
authors.initials: MA
authors.lastname: Mehrgardi
authors.forename: Emilia
authors.initials: E
authors.lastname: Wiechec
authors.forename: Anthony P F
authors.initials: AP
authors.lastname: Turner
authors.forename: Ashutosh
authors.initials: A
authors.lastname: Tiwari
mesh_terms: D052158:Aptamers, Peptide / Q000737:chemistry*; D015374:Biosensing Techniques; D006528:Carcinoma, Hepatocellular / Q000737:chemistry*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D006801:Humans
chemical_list: D052158:Aptamers, Peptide