title: Cloning of TTG1 gene and PCR identification of genomes A, B and C in Brassica species.
abstract: Arabidopsis Transparent Testa Glabra 1 (TTG1) genes were cloned from three diploid Brassica species (B. rapa, B. nigra and B. oleracea) and two amphidiploids species (B. juncea and B. carinata) by homology cloning. TTG1 homologues identified in all the accessions of the investigated species had a coding sequence of 1,014 bp. One copy was obtained from each diploid species and two copies from each amphidiploid species. Combined analysis of the TTG1 sequences cloned in this study with those obtained from public databases demonstrated that three, forty-five and seven nucleotides were specific variations in TTG1 genes from genomes A, B and C, respectively. Primers designed with genome-specific nucleotide variations were able to distinguish among TTG1 genes originating from genomes A, B and C in Brassica. Therefore, the TTG1 gene could serve as a candidate marker gene to detect the pollen flow of Brassica and provide an alternative method for the detection of pollen drift and risk assessment of gene flow in Brassica species.
authors.affiliation: School of Life Science, Hunan University of Science and Technology, Xiangtan, 411201, Hunan, China, ymljack@126.com.
authors.forename: Mingli
authors.initials: M
authors.lastname: Yan
authors.forename: Xianjun
authors.initials: X
authors.lastname: Liu
authors.forename: Chunyun
authors.initials: C
authors.lastname: Guan
authors.forename: Lili
authors.initials: L
authors.lastname: Liu
authors.forename: Jianhua
authors.initials: J
authors.lastname: Xiang
authors.forename: Ying
authors.initials: Y
authors.lastname: Lu
authors.forename: Zhongsong
authors.initials: Z
authors.lastname: Liu
mesh_terms: D001937:Brassica / Q000145:classification / Q000235:genetics*; D003001:Cloning, Molecular; D051456:Gene Flow; D017343:Genes, Plant; D014644:Genetic Variation; D018745:Genome, Plant; D008958:Models, Molecular; D010802:Phylogeny; D011058:Pollen / Q000235:genetics; D016133:Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid
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