title: Recovery of amorphous polyhydroxybutyrate granules from Cupriavidus necator cells grown on used cooking oil.
abstract: Used cooking oil (UCO) was employed as the sole carbon source for the production of polyhydroxybutyrate (PHB) by cultivation in batch mode of Cupriavidus necator DSM 428. The produced biomass was used for extraction of the PHB granules with a solvent-free approach using sodium dodecyl sulfate (SDS), ethylenediaminetetraacetic acid (EDTA), and the enzyme Alcalase in an aqueous medium. The recovered PHB granules showed a degree of purity higher than 90% and no crystallization (i.e., granules were recovered in their 'native' amorphous state) as demonstrated by wide angle X-ray diffraction (WAXS). Granules were characterized according to their thermal properties and stability by differential scanning calorimetry (DSC), and thermogravimetric analysis (TGA). Results show that UCO can be used as a renewable resource to produce amorphous PHB granules with excellent properties in a biocompatible manner.
authors.affiliation: Department of Chemistry "G. Ciamician", Alma Mater Studiorum - University of Bologna, via Selmi 2, I-40126 Bologna, Italy.
authors.forename: Lucrezia
authors.initials: L
authors.lastname: Martino
authors.affiliation: REQUIMTE/CQFB, Chemistry Department, FCT/Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.
authors.forename: Madalena V
authors.initials: MV
authors.lastname: Cruz
authors.affiliation: DICAM, Department of Civil, Chemical, Environmental and Materials Engineering, Alma Mater Studiorum - University of Bologna, via Terracini 28, I-40131 Bologna, Italy. Electronic address: Alberto.Scoma@UGent.be.
authors.forename: Alberto
authors.initials: A
authors.lastname: Scoma
authors.affiliation: REQUIMTE/CQFB, Chemistry Department, FCT/Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.
authors.forename: Filomena
authors.initials: F
authors.lastname: Freitas
authors.affiliation: DICAM, Department of Civil, Chemical, Environmental and Materials Engineering, Alma Mater Studiorum - University of Bologna, via Terracini 28, I-40131 Bologna, Italy.
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Bertin
authors.affiliation: Department of Chemistry "G. Ciamician", Alma Mater Studiorum - University of Bologna, via Selmi 2, I-40126 Bologna, Italy.
authors.forename: Mariastella
authors.initials: M
authors.lastname: Scandola
authors.affiliation: REQUIMTE/CQFB, Chemistry Department, FCT/Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.
authors.forename: Maria A M
authors.initials: MA
authors.lastname: Reis
mesh_terms: D018533:Biomass; D001704:Biopolymers / Q000737:chemistry / Q000378:metabolism; D019149:Bioreactors; D003296:Cooking; D020118:Cupriavidus necator / Q000378:metabolism*; D005285:Fermentation; D006885:Hydroxybutyrates / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D059625:Liquid-Liquid Extraction; D010938:Plant Oils / Q000737:chemistry*; D013818:Thermogravimetry
chemical_list: D001704:Biopolymers; D006885:Hydroxybutyrates; D010938:Plant Oils
keywords: Biocompatible extraction; Biowaste; Native amorphous granule; Polyhydroxyalkanoate (PHA); Spent oil; Used cooking oil (UCO)