title: The role of phospholipase D1 in liver fibrosis induced by dimethylnitrosamine in vivo.
abstract: BACKGROUND
  Phospholipase D (PLD) has been proved to be involved in regulating function of fibroblasts and might play a role in mediating organic fibrosis.
  
  
  OBJECTIVE
  To investigate the role and mechanism of PLD on dimethylnitrosamine (DMN)-induced rat liver fibrosis.
  
  
  METHODS
  Fifty-five male Wistar rats were divided into normal control group, DMN model group, N-methylethanolamine (MEA) control group, and MEA-intervention group. We observed the effects of MEA, a PLD inhibitor on the development and progression of rat liver fibrosis by comparing the physical and biochemical indexes, tissue pathology, PLD activity, and typical markers and cytokines related to fibrosis in the four groups.
  
  
  RESULTS
  Accompanied by the down-regulation of PLD1 expression, the MEA-intervention group had improved outcomes compared with the DMN model group in terms of spleen weight, spleen/weight index, serum and tissue biochemical indexes, tissue hydroxyproline, and tissue pathology. The MEA-intervention group had lower TIMP1, COL1A1, and higher MMPs expression level than the DMN model group. The activity of PLD and PLD1, α-SMA expression level in the MEA-intervention group was much lower than those in the DMN model group. There was no significant difference between the two groups in the expression level of TGF-β1 and MCP1. Meanwhile, there were no significant differences between normal control group and MEA control group in the parameters stated above.
  
  
  CONCLUSIONS
  Phospholipase D1 may play an important role in the development and progression of rat liver fibrosis. Inhibition of PLD may become a new strategy to prevent or alleviate liver fibrosis.
authors.affiliation: Department of Gastroenterology and Digestive diseases Institute, Shanghai Tongji Hospital, Tongji University School of Medicine, Shanghai, 200065, China.
authors.forename: Xinyan
authors.initials: X
authors.lastname: Zhu
authors.forename: Ruilin
authors.initials: R
authors.lastname: Liu
authors.forename: Dapeng
authors.initials: D
authors.lastname: Kuang
authors.forename: Jingqi
authors.initials: J
authors.lastname: Liu
authors.forename: Xiaomeng
authors.initials: X
authors.lastname: Shi
authors.forename: Tingting
authors.initials: T
authors.lastname: Zhang
authors.forename: Yu
authors.initials: Y
authors.lastname: Zeng
authors.forename: Xianghua
authors.initials: X
authors.lastname: Sun
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Wenzhuo
authors.initials: W
authors.lastname: Yang
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D004128:Dimethylnitrosamine; D018450:Disease Progression; D015536:Down-Regulation; D008099:Liver / Q000473:pathology; D008103:Liver Cirrhosis / Q000139:chemically induced / Q000201:enzymology* / Q000473:pathology; D008297:Male; D010739:Phospholipase D / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D053773:Transforming Growth Factor beta1 / Q000378:metabolism
chemical_list: D000199:Actins; D004128:Dimethylnitrosamine; D010739:Phospholipase D; D053773:Transforming Growth Factor beta1; C508962:Tgfb1 protein, rat; C498862:Acta2 protein, rat; C105606:phospholipase D2; C106801:phospholipase D1
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