title: Progenitor cell dynamics in the Newt Telencephalon during homeostasis and neuronal regeneration.
abstract: The adult newt brain has a marked neurogenic potential and is highly regenerative. Ventricular, radial glia-like ependymoglia cells give rise to neurons both during normal homeostasis and after injury, but subpopulations among ependymoglia cells have not been defined. We show here that a substantial portion of GFAP(+) ependymoglia cells in the proliferative hot spots of the telencephalon has transit-amplifying characteristics. In contrast, proliferating ependymoglia cells, which are scattered along the ventricular wall, have stem cell features in terms of label retention and insensitivity to AraC treatment. Ablation of neurons remodels the proliferation dynamics and leads to de novo formation of regions displaying features of neurogenic niches, such as the appearance of cells with transit-amplifying features and proliferating neuroblasts. The results have implication both for our understanding of the evolutionary diversification of radial glia cells as well as the processes regulating neurogenesis and regeneration in the adult vertebrate brain.
authors.affiliation: Department of Cell and Molecular Biology, Karolinska Institutet, Berzelius väg 35, 171 77 Stockholm, Sweden.
authors.forename: Matthew
authors.initials: M
authors.lastname: Kirkham
authors.affiliation: Department of Cell and Molecular Biology, Karolinska Institutet, Berzelius väg 35, 171 77 Stockholm, Sweden.
authors.forename: L Shahul
authors.initials: LS
authors.lastname: Hameed
authors.affiliation: Department of Cell and Molecular Biology, Karolinska Institutet, Berzelius väg 35, 171 77 Stockholm, Sweden.
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Berg
authors.affiliation: Department of Cell and Molecular Biology, Karolinska Institutet, Berzelius väg 35, 171 77 Stockholm, Sweden.
authors.forename: Heng
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Cell and Molecular Biology, Karolinska Institutet, Berzelius väg 35, 171 77 Stockholm, Sweden.
authors.forename: András
authors.initials: A
authors.lastname: Simon
mesh_terms: D063928:Ependymoglial Cells / Q000166:cytology / Q000378:metabolism; D027341:Excitatory Amino Acid Transporter 1 / Q000378:metabolism; D006706:Homeostasis; D009416:Nerve Regeneration; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D009474:Neurons / Q000166:cytology* / Q000502:physiology*; D051880:Receptors, Notch / Q000378:metabolism; D055748:SOXB1 Transcription Factors / Q000378:metabolism; D015398:Signal Transduction; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism*; D013687:Telencephalon / Q000166:cytology* / Q000502:physiology*
chemical_list: D027341:Excitatory Amino Acid Transporter 1; D009419:Nerve Tissue Proteins; D051880:Receptors, Notch; D055748:SOXB1 Transcription Factors
references.citation: Cell Stem Cell. 2011 Apr 8;8(4):426-33
references.pmid: 21474106
references.citation: Development. 2010 Dec;137(24):4127-34
references.pmid: 21068061
references.citation: Nat Neurosci. 2009 Mar;12(3):259-67
references.pmid: 19234458
references.citation: Glia. 2012 Mar;60(3):343-57
references.pmid: 22105794
references.citation: Bioessays. 2007 Aug;29(8):745-57
references.pmid: 17621643
references.citation: Dev Biol. 2006 Jul 1;295(1):278-93
references.pmid: 16828638
references.citation: Glia. 2010 Aug 15;58(11):1345-63
references.pmid: 20607866
references.citation: Annu Rev Neurosci. 2009;32:149-84
references.pmid: 19555289
references.citation: EMBO Rep. 2002 Jul;3(7):688-94
references.pmid: 12101103
references.citation: Curr Opin Neurobiol. 2012 Oct;22(5):754-61
references.pmid: 22503352
references.citation: Development. 2007 Aug;134(15):2881-7
references.pmid: 17611231
references.citation: Nature. 2001 Feb 8;409(6821):714-20
references.pmid: 11217860
references.citation: Neural Dev. 2013 Jan 17;8:1
references.pmid: 23327114
references.citation: J Neurosci. 2012 Apr 18;32(16):5654-66
references.pmid: 22514327
references.citation: J Neurosci. 2010 Oct 13;30(41):13794-807
references.pmid: 20943920
references.citation: Brain Res Dev Brain Res. 1996 May 31;93(1-2):49-61
references.pmid: 8804691
references.citation: CNS Neurol Disord Drug Targets. 2007 Oct;6(5):326-35
references.pmid: 18045161
references.citation: Dev Cell. 2012 Dec 11;23(6):1230-7
references.pmid: 23168169
references.citation: J Comp Neurol. 2004 Oct 25;478(4):359-78
references.pmid: 15384070
references.citation: Science. 1992 Mar 27;255(5052):1707-10
references.pmid: 1553558
references.citation: J Neurosci. 2010 Aug 4;30(31):10484-92
references.pmid: 20685991
references.citation: Regen Med. 2006 Mar;1(2):207-16
references.pmid: 17465804
references.citation: Development. 2011 Nov;138(22):4831-41
references.pmid: 22007133
references.citation: J Neurosci. 2010 Jun 9;30(23):7961-74
references.pmid: 20534844
references.citation: J Neurosci. 2010 Mar 3;30(9):3489-98
references.pmid: 20203209
references.citation: Development. 2000 Dec;127(24):5253-63
references.pmid: 11076748
references.citation: Neurosci Lett. 2011 Jun 15;497(1):11-6
references.pmid: 21515337
references.citation: Dev Neurosci. 2006;28(1-2):118-27
references.pmid: 16508309
references.citation: Glia. 2010 May;58(7):870-88
references.pmid: 20155821
references.citation: Neurobiol Dis. 2010 Feb;37(2):267-74
references.pmid: 19909815
references.citation: Dis Model Mech. 2012 Mar;5(2):200-9
references.pmid: 22028327
references.citation: Exp Cell Res. 2013 May 1;319(8):1187-97
references.pmid: 23454602
references.citation: J Neurosci. 2009 May 13;29(19):6142-53
references.pmid: 19439592
references.citation: J Neural Transm Suppl. 2009;(73):279-85
references.pmid: 20411786
references.citation: Cell. 1999 Jun 11;97(6):703-16
references.pmid: 10380923
references.citation: Brain Res. 1979 Feb 2;161(2):303-10
references.pmid: 31966
references.citation: Dev Genes Evol. 2013 Mar;223(1-2):131-47
references.pmid: 23179636
references.citation: J Comp Neurol. 1995 Jan 2;351(1):51-61
references.pmid: 7896939