title: The application of targeted mass spectrometry-based strategies to the detection and localization of post-translational modifications.
abstract: This review describes some of the more interesting and imaginative ways in which mass spectrometry has been utilized to study a number of important post-translational modifications over the past two decades; from circa 1990 to 2013. A diverse range of modifications is covered, including citrullination, sulfation, hydroxylation and sumoylation. A summary of the biological role of each modification described, along with some brief mechanistic detail, is also included. Emphasis has been placed on strategies specifically aimed at detecting target modifications, as opposed to more serendipitous modification discovery approaches, which rely upon straightforward product ion scanning methods. The authors have intentionally excluded from this review both phosphorylation and glycosylation since these major modifications have been extensively reviewed elsewhere.
authors.affiliation: CRUK Manchester Institute, University of Manchester, Wilmslow Road, Manchester, M20 4BX, UK.|School of Chemistry, University of Manchester, Brunswick Street, Manchester, M13 9SU, UK.
authors.forename: Navin
authors.initials: N
authors.lastname: Chicooree
authors.affiliation: Centre for Advanced Discovery and Experimental Therapeutics (CADET), Central Manchester University Hospitals NHS Foundation Trust, Manchester Academic Health Sciences Centre, Manchester, M13 9WL, UK.|Institute of Human Development, Faculty of Medical and Human Sciences, University of Manchester, Manchester, UK.
authors.forename: Richard D
authors.initials: RD
authors.lastname: Unwin
authors.affiliation: CRUK Manchester Institute, University of Manchester, Wilmslow Road, Manchester, M20 4BX, UK.
authors.forename: John R
authors.initials: JR
authors.lastname: Griffiths
mesh_terms: D000107:Acetylation; D000595:Amino Acid Sequence; D000818:Animals; D006801:Humans; D006900:Hydroxylation; D013058:Mass Spectrometry / Q000379:methods*; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009574:Nitro Compounds / Q000032:analysis; D009603:Nitroso Compounds / Q000032:analysis; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D012984:Software; D013451:Sulfonic Acids / Q000032:analysis
chemical_list: D009574:Nitro Compounds; D009603:Nitroso Compounds; D011506:Proteins; D013451:Sulfonic Acids
keywords: PTMs; acetylation; citrullination; hydroxylation; mass spectrometry; nitration; nitrosylation; sulfation; sumoylation; ubiquitination