title: Down-regulation of FcεRI-mediated CD63 basophil response during short-term VIT determined venom-nonspecific desensitization.
abstract: BACKGROUND
  We recently showed a desensitization of FcεRI-mediated basophil response after short-term VIT. Our aim was to evaluate the allergen specificity of this desensitization.
  
  
  METHODS
  In 11 Hymenoptera-venom double positive subjects, basophil threshold sensitivity (CD-sens) to anti-FcεRI, honeybee, and Vespula venom was assessed at the beginning and just before the first maintenance dose (MD) of single ultra-rush VIT. In some patients we also monitored CD-sens to rApi m 1 and/or rVes v 5 or other co-sensitizations (i.e., grass pollen). In additional 7 patients, basophils were stripped and sensitized with house dust mite (HDM) IgEs at the same time points.
  
  
  RESULTS
  We demonstrated a marked reduction of CD-sens to anti-FcεRI and VIT-specific venom before the first MD in all 18 subjects included. Furthermore, in 10 out of 11 double positive subjects, a significant and comparable decrease before the first MD was also evident for non-VIT venom; this nonspecific decrease was further supported by the opposite recombinant species-specific major allergen. In one subject with additional grass pollen allergy, a decrease of CD-sens to grass allergen was also demonstrated. Similarly, in 7 cases of patients with passively HDM-sensitized basophils, a significant reduction of CD-sens was also evident to de novo sensitized HDM allergen.
  
  
  CONCLUSIONS
  Short-term VIT induced basophil desensitization to VIT-specific as well as to VIT-nonspecific venom. As opposed to long-term VIT, which induces venom-specific changes, the effect of short-term VIT seems to be venom-nonspecific.
authors.affiliation: University Clinic of Respiratory and Allergic Diseases Golnik, Golnik, Slovenia.
authors.forename: Nina
authors.initials: N
authors.lastname: Čelesnik Smodiš
authors.affiliation: University Clinic of Respiratory and Allergic Diseases Golnik, Golnik, Slovenia.
authors.forename: Mira
authors.initials: M
authors.lastname: Šilar
authors.affiliation: University Clinic of Respiratory and Allergic Diseases Golnik, Golnik, Slovenia.
authors.forename: Renato
authors.initials: R
authors.lastname: Eržen
authors.affiliation: University Clinic of Respiratory and Allergic Diseases Golnik, Golnik, Slovenia.
authors.forename: Matija
authors.initials: M
authors.lastname: Rijavec
authors.affiliation: University Clinic of Respiratory and Allergic Diseases Golnik, Golnik, Slovenia.
authors.forename: Mitja
authors.initials: M
authors.lastname: Košnik
authors.affiliation: University Clinic of Respiratory and Allergic Diseases Golnik, Golnik, Slovenia.
authors.forename: Peter
authors.initials: P
authors.lastname: Korošec
mesh_terms: D000328:Adult; D000485:Allergens; D000818:Animals; D001491:Basophils / Q000187:drug effects / Q000378:metabolism; D001514:Bee Venoms / Q000627:therapeutic use*; D001516:Bees; D002462:Cell Membrane / Q000378:metabolism; D015536:Down-Regulation; D005260:Female; D006801:Humans; D006927:Hymenoptera; D007073:Immunoglobulin E / Q000276:immunology; D007167:Immunotherapy / Q000379:methods*; D008297:Male; D008875:Middle Aged; D008925:Mites; D006109:Poaceae; D011058:Pollen / Q000276:immunology; D017452:Receptors, IgG / Q000378:metabolism*; D060149:Tetraspanin 30 / Q000378:metabolism*; D055815:Young Adult
chemical_list: D000485:Allergens; D001514:Bee Venoms; C554586:CD63 protein, human; C489017:FCGR1A protein, human; D017452:Receptors, IgG; D060149:Tetraspanin 30; D007073:Immunoglobulin E
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