title: The target gene carrying validity to HePG2 cells with the brush-like glutathione modified chitosan compound.
abstract: The grafting modified chitosan with L-glutathione (GSH) end capped PEG brush-like poly [poly(ethylene glycol) methacrylate] (PMPEG), CS-PMPEG-GSH, as the pDNA condensed vector material could result in a much higher transfection efficiency and lower cytotoxity for NIH3T3 cells. In this work, we have further examined the morphology stabilities of CS-PMPEG-GSH/pDNA vectors at different medium pH values and in the presence of serum protein in detail. And then the targeted characters for HepG2 cells have been probed by tracing the cell uptake behavior and transfection efficiency.
authors.affiliation: Key Laboratory of Functional Polymer Materials (Nankai University), Ministry of Education, Institute of Polymer Chemistry, College of Chemistry, Weijin Road, No. 94, Tianjin 300071, China.
authors.forename: Congxin
authors.initials: C
authors.lastname: Li
authors.affiliation: Key Laboratory of Functional Polymer Materials (Nankai University), Ministry of Education, Institute of Polymer Chemistry, College of Chemistry, Weijin Road, No. 94, Tianjin 300071, China.
authors.forename: Dezhong
authors.initials: D
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Functional Polymer Materials (Nankai University), Ministry of Education, Institute of Polymer Chemistry, College of Chemistry, Weijin Road, No. 94, Tianjin 300071, China.
authors.forename: Yuling
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Department of Biochemistry and Molecular Biology, College of Life Science, Nankai University, Weijin Road, No. 94, Tianjin 300071, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Department of Biochemistry and Molecular Biology, College of Life Science, Nankai University, Weijin Road, No. 94, Tianjin 300071, China.
authors.forename: Jiatong
authors.initials: J
authors.lastname: Chen
authors.affiliation: Key Laboratory of Functional Polymer Materials (Nankai University), Ministry of Education, Institute of Polymer Chemistry, College of Chemistry, Weijin Road, No. 94, Tianjin 300071, China.
authors.forename: Zhengpu
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Functional Polymer Materials (Nankai University), Ministry of Education, Institute of Polymer Chemistry, College of Chemistry, Weijin Road, No. 94, Tianjin 300071, China.
authors.forename: Tianying
authors.initials: T
authors.lastname: Guo
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000008:administration & dosage* / Q000737:chemistry; D004247:DNA / Q000008:administration & dosage* / Q000737:chemistry; D018014:Gene Transfer Techniques; D005978:Glutathione / Q000008:administration & dosage* / Q000737:chemistry; D049452:Green Fluorescent Proteins / Q000235:genetics; D006367:HeLa Cells; D056945:Hep G2 Cells; D006801:Humans; D008689:Methacrylates / Q000008:administration & dosage* / Q000737:chemistry; D051379:Mice; D041681:NIH 3T3 Cells; D010957:Plasmids; D011092:Polyethylene Glycols / Q000008:administration & dosage* / Q000737:chemistry
chemical_list: D008689:Methacrylates; C485184:enhanced green fluorescent protein; C524499:polyethylene glycol methacrylate; D049452:Green Fluorescent Proteins; D011092:Polyethylene Glycols; D004247:DNA; D048271:Chitosan; D005978:Glutathione