title: Melittin ameliorates the inflammation of organs in an amyotrophic lateral sclerosis animal model.
abstract: Amyotrophic lateral sclerosis (ALS) is a devastating progressive neurodegenerative disorder characterized by a selective loss of motor neurons in the spinal cord, brainstem, and motor cortex, leading to weakness of the limb and bulbar muscles. Although the immediate cause of death in ALS is the destruction of motor neurons, ALS is a multi-organ disease that also affects the lungs, spleen, and liver. Melittin is one of components of bee venom and has anti-neuroinflammatory effects in the spinal cord, as shown in an ALS animal model. To investigate the effects of melittin on inflammation in the lungs and spleen, we used hSOD1(G93A) transgenic mice that are mimic for ALS. Melittin treatment reduced the expression of inflammatory proteins, including Iba-1 and CD14 by 1.9- and 1.3-fold (p<0.05), respectively, in the lungs of symptomatic hSOD1(G93A) transgenic mice. In the spleen, the expression of CD14 and COX2 that are related to inflammation were decreased by 1.4 fold (p<0.05) and cell survival proteins such as pERK and Bcl2 were increased by 1.3- and 1.5-fold (p<0.05) in the melittin-treated hSOD1G93A transgenic mice. These findings suggest that melittin could be a candidate to regulate the immune system in organs affected by ALS.
authors.affiliation: Department of Medical Research, Korea Institute of Oriental Medicine, Daejeon 305-811, Korea.
authors.forename: Sun-Hwa
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Department of Medical Research, Korea Institute of Oriental Medicine, Daejeon 305-811, Korea.
authors.forename: Sun-Mi
authors.initials: SM
authors.lastname: Choi
authors.affiliation: Department of Medical Research, Korea Institute of Oriental Medicine, Daejeon 305-811, Korea.
authors.forename: Eun Jin
authors.initials: EJ
authors.lastname: Yang
keywords: Amyotrophic lateral sclerosis (ALS); Melittin; hSOD1G93A; inflammation
references.citation: PLoS One. 2008 Jul 23;3(7):e2740
references.pmid: 18648532
references.citation: J Neuroinflammation. 2013 Sep 26;10:119
references.pmid: 24067070
references.citation: Curr Pharm Des. 2007;13(18):1925-8
references.pmid: 17584117
references.citation: Neuropathol Appl Neurobiol. 1990 Oct;16(5):401-10
references.pmid: 2263315
references.citation: Nat Neurosci. 2006 Feb;9(2):268-75
references.pmid: 16415867
references.citation: Arthritis Rheum. 2004 Nov;50(11):3504-15
references.pmid: 15529353
references.citation: Curr Opin Immunol. 2003 Dec;15(6):683-9
references.pmid: 14630203
references.citation: Nat Neurosci. 2007 Nov;10(11):1355-60
references.pmid: 17965655
references.citation: Neurology. 1996 Oct;47(4):1060-4
references.pmid: 8857745
references.citation: PLoS One. 2011;6(8):e22374
references.pmid: 21829620
references.citation: Science. 1972 Jul 28;177(4046):314-22
references.pmid: 4113805
references.citation: Neurology. 2001 Oct 9;57(7):1282-9
references.pmid: 11591849
references.citation: Acta Neurol Scand. 1988 Dec;78(6):449-54
references.pmid: 3265563
references.citation: Biochim Biophys Acta. 2006 Nov-Dec;1762(11-12):1083-93
references.pmid: 16624536
references.citation: Trends Immunol. 2006 Jul;27(7):322-7
references.pmid: 16735139
references.citation: J Neuroimmunol. 2005 Feb;159(1-2):215-24
references.pmid: 15652422
references.citation: Res Vet Sci. 2008 Apr;84(2):159-65
references.pmid: 17559895
references.citation: J Biol Chem. 2009 Feb 6;284(6):3804-13
references.pmid: 19074436
references.citation: J Neuropathol Exp Neurol. 2004 Feb;63(2):113-9
references.pmid: 14989597
references.citation: Science. 1994 Jun 17;264(5166):1772-5
references.pmid: 8209258
references.citation: Amyotroph Lateral Scler Other Motor Neuron Disord. 2004 Dec;5(4):213-9
references.pmid: 15799549
references.citation: Nature. 1993 Mar 4;362(6415):59-62
references.pmid: 8446170
references.citation: J Neuroinflammation. 2011 Jun 20;8:69
references.pmid: 21682930
references.citation: J Neuroimmunol. 2006 Oct;179(1-2):87-93
references.pmid: 16857270
references.citation: J Pharmacol Exp Ther. 2006 May;317(2):627-34
references.pmid: 16401728
references.citation: Neurodegener Dis. 2011;8(6):504-14
references.pmid: 21849797
references.citation: Hum Immunol. 2008 Nov;69(11):708-14
references.pmid: 18817827
references.citation: Am J Pathol. 1992 Mar;140(3):691-707
references.pmid: 1347673
references.citation: Biosci Rep. 2007 Oct;27(4-5):189-223
references.pmid: 17139559
references.citation: Nat Rev Neurosci. 2001 Nov;2(11):806-19
references.pmid: 11715057
references.citation: Neuron. 2006 Oct 5;52(1):39-59
references.pmid: 17015226
references.citation: Muscle Nerve. 2002 Oct;26(4):459-70
references.pmid: 12362410
references.citation: Clin Exp Immunol. 1982 Apr;48(1):137-46
references.pmid: 6282508
references.citation: J Inflamm (Lond). 2008 May 29;5:7
references.pmid: 18507870
references.citation: Curr Opin Investig Drugs. 2002 Dec;3(12):1720-4
references.pmid: 12528305
references.citation: Trends Immunol. 2008 May;29(5):211-9
references.pmid: 18328784