title: Increased p16 DNA methylation in mouse thymic lymphoma induced by irradiation.
abstract: DNA methylation is an important part of epigenetics. In this study, we examined the methylation state of two CpG islands in the promoter of the p16 gene in radiation-induced thymic lymphoma samples. The mRNA and protein levels of P16 were significantly reduced in radiation-induced thymic lymphoma tissue samples. Twenty-three CpG sites of the CpG islands in the p16 promoter region were detected, and the methylation percentages of -71, -63, -239, -29, -38, -40, -23, 46 CpG sites were significantly higher in radiation-induced thymic lymphoma tissue samples than those in matched non-irradiated thymus tissue samples. This study provides new evidence for the methylation state of p16 in the radiation-induced thymic lymphoma samples, which suggests that the methylation of these CpG sites in the p16 promoter may reduce its expression in the thymic lymphoma after irradiation.
authors.affiliation: Beihua University, Jinlin, China.
authors.forename: Wengang
authors.initials: W
authors.lastname: Song
authors.affiliation: Department of Toxicology, School of Public Health, Tianjin Medical University, Tianjin, China.
authors.forename: Yongzhe
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Toxicology, School of Public Health, Jilin University, Changchun, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Ministry of Health, Key Laboratory of Radiobiology, Jilin University, Changchun, China, National Laboratory of Medical Molecular Biology, Tsinghua University, Beijing, PR China.
authors.forename: Cong
authors.initials: C
authors.lastname: Zhang
authors.affiliation: College of Animal Sciences, Jilin University, Changchun, China.
authors.forename: Bao
authors.initials: B
authors.lastname: Yuan
authors.affiliation: Department of Plastic and Reconstructive Surgery, China-Japan Union Hospital of Jilin University, Changchun, China.
authors.forename: Lianbo
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Ministry of Health, Key Laboratory of Radiobiology, Jilin University, Changchun, China, National Laboratory of Medical Molecular Biology, Tsinghua University, Beijing, PR China.
authors.forename: Shilong
authors.initials: S
authors.lastname: Sun
mesh_terms: D000818:Animals; D001483:Base Sequence; D018899:CpG Islands / Q000235:genetics / Q000528:radiation effects; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000235:genetics*; D019175:DNA Methylation / Q000528:radiation effects*; D015536:Down-Regulation / Q000235:genetics / Q000528:radiation effects; D008223:Lymphoma / Q000235:genetics* / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009381:Neoplasms, Radiation-Induced / Q000235:genetics* / Q000473:pathology; D013953:Thymus Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: D019941:Cyclin-Dependent Kinase Inhibitor p16
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