title: The Study of Pentoxifylline Drug Effects on Renal Apoptosis and BCL-2 Gene Expression Changes Following Ischemic Reperfusion Injury in Rat.
abstract: Ischemia Reperfusion injury is the tissue damage caused when blood supply returns to the tissue after a period of ischemia or lack of oxygen. In this study, the effect of pentoxyfylline on BCL-2 gene expression changes and cell injury in kidney of rat following Ischemia Reperfusion were evaluated. In this experimental study, 20 male wistar rats with average weight of 250-300 g were selected and then were accidently divided them on two tenth group of control and treatment groups. In the control group, celiotomy was performed by ventral midline incision. The left kidney was isolated, and then both the renal artery and vein were obstructed. After 60 minutes of warm ischemia, vessel obstruction resolved and the right kidney was removed. 72 hours after reperfusion, tissue samples were taken from left kidney for Tunel assay. We used quantitative real time PCR for detection of BCL-2 gene expression in treated groups and then compared them to control samples. In the treatment group, the cell death changes, showed lower level than the control group. The results also showed the BCL-2 gene expression was declined in ischemia group as campared to PNT drug group. The pentoxyfylline might have a role in control of apoptosis result from Ischemia- reperfusion and quantitative real-time PCR can be used as a direct method for detection BCL-2 gene expression in tested samples and normal samples.
authors.affiliation: Department of Pharmacology and Toxicology Pharmaceutical Sciences Branch Islamic Azad University, Tehran, Iran.
authors.forename: Mehrdad
authors.initials: M
authors.lastname: Hashemi
keywords: BCL-2 gene; Ischemia; Pentoxyfylline; Real-Time PCR
references.citation: Dig Dis Sci. 2004 Sep;49(9):1359-77
references.pmid: 15481305
references.citation: J Am Soc Nephrol. 2001 May;12(5):973-982
references.pmid: 11316856
references.citation: Nephrol Dial Transplant. 2001 Mar;16(3):518-24
references.pmid: 11239025
references.citation: Brain Res Mol Brain Res. 2004 Aug 23;127(1-2):10-26
references.pmid: 15306117
references.citation: Surgery. 2002 Jun;131(6):654-62
references.pmid: 12075178
references.citation: J Urol. 1999 Jul;162(1):196-203
references.pmid: 10379787
references.citation: Neurol Clin. 2006 Feb;24(1):1-21
references.pmid: 16443127
references.citation: BJU Int. 2000 Jun;85(9):1007-13
references.pmid: 10848685
references.citation: Prog Clin Biol Res. 1991;367:207-13
references.pmid: 1924429
references.citation: Rev Bras Cir Cardiovasc. 2010 Oct-Dec;25(4):575-84
references.pmid: 21340389
references.citation: Iran J Pharm Res. 2013 Summer;12(3):521-7
references.pmid: 24250658
references.citation: J Pharmacol Exp Ther. 2004 Sep;310(3):890-5
references.pmid: 15201342
references.citation: J Neurosci. 2008 Jun 4;28(23):5965-75
references.pmid: 18524901
references.citation: Curr Opin Pediatr. 2005 Apr;17(2):193-9
references.pmid: 15800411
references.citation: Kidney Int. 2004 Aug;66(2):486-91
references.pmid: 15253694
references.citation: Acta Cir Bras. 2008 Jan-Feb;23(1):29-35
references.pmid: 18278390
references.citation: Eur J Trauma Emerg Surg. 2007 Dec;33(6):600-12
references.pmid: 26815087
references.citation: Rev Med Interne. 2006 May;27(5):400-5
references.pmid: 16309798
references.citation: Endocrinology. 2007 Mar;148(3):1131-43
references.pmid: 17138646
references.citation: Am J Physiol. 1997 May;272(5 Pt 2):F640-7
references.pmid: 9176375
references.citation: J Transl Med. 2009 Nov 17;7:97
references.pmid: 19919699
references.citation: Acta Cir Bras. 2005 Jul-Aug;20(4):336-43
references.pmid: 16186955
references.citation: Am J Physiol Cell Physiol. 2008 Feb;294(2):C460-6
references.pmid: 18077608
references.citation: J Clin Invest. 1996 Feb 15;97(4):1056-63
references.pmid: 8613529
references.citation: Acta Pharmacol Sin. 2004 Jul;25(7):869-75
references.pmid: 15210059
references.citation: Arthritis Rheum. 1989 Jan;32(1):1-9
references.pmid: 2643434
references.citation: J Am Soc Nephrol. 2000 Mar;11(3):454-467
references.pmid: 10703669
references.citation: Iran J Pharm Res. 2013 Summer;12(3):495-501
references.pmid: 24250655
references.citation: Cell Death Differ. 2010 Jun;17(6):922-30
references.pmid: 20010783
references.citation: Pathol Biol (Paris). 2002 Dec;50(10):599-607
references.pmid: 12504369
references.citation: Transplantation. 1999 Mar 27;67(6):792-800
references.pmid: 10199725
references.citation: Int J Oncol. 2002 Jul;21(1):165-70
references.pmid: 12063564
references.citation: Shock. 2004 May;21(5):401-9
references.pmid: 15087815