title: Splenic B-1a Cells Expressing CD138 Spontaneously Secrete Large Amounts of Immunoglobulin in Naïve Mice.
abstract: B-1a cells constitutively secrete natural antibody that provides immediate protection against microbial pathogens and functions homeostatically to speed removal of apoptotic cell debris. Although B-1a cells are especially prominent in the peritoneal and pleural cavities, some B-1a cells reside in the spleen. A small subset of splenic B-1a cells in naïve, unimmunized mice express CD138, a recognized plasma cell antigen, whereas the bulk of splenic B-1a cells are CD138 negative. Splenic B-1a cells in toto have been shown to generate much more antibody per cell than peritoneal B-1a cells; however, specific functional information regarding CD138(+) splenic B-1a cells has been lacking. Here, we find a higher proportion of CD138(+) splenic B-1a cells spontaneously secrete more IgM as compared to CD138(-) B-1a cells. Moreover, IgM secreted by CD138(+) splenic B-1a cells is skewed with respect to N-region addition, and some aspects of VH and JH utilization, as compared to CD138(-) splenic B-1a cells and peritoneal B-1a cells. The small population of CD138(+) splenic B-1a cells is likely responsible for a substantial portion of natural IgM and differs from IgM produced by other B-1a cell subsets.
authors.affiliation: Center for Oncology and Cell Biology, The Feinstein Institute for Medical Research , Manhasset, NY , USA.
authors.forename: Nichol E
authors.initials: NE
authors.lastname: Holodick
authors.affiliation: Center for Oncology and Cell Biology, The Feinstein Institute for Medical Research , Manhasset, NY , USA.
authors.forename: Teresa
authors.initials: T
authors.lastname: Vizconde
authors.affiliation: Center for Oncology and Cell Biology, The Feinstein Institute for Medical Research , Manhasset, NY , USA ; Departments of Medicine and Molecular Medicine, Hofstra North Shore-LIJ School of Medicine , Manhasset, NY , USA.
authors.forename: Thomas L
authors.initials: TL
authors.lastname: Rothstein
keywords: B lymphocytes; B-1 cells; IgM; antibody
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