title: Reproductive toxicity in acrylamide-treated female mice.
abstract: We investigated the reproductive toxicity of acrylamide in female mice. The results from immunohistochemistry provided evidence that nitric oxide synthase (NOS) signaling was involved in the process of follicular development and atresia. Oral administration of acrylamide to female mice led to significantly reduced body weights, organ weights and the number of corpora lutea (P<0.05). Serum progesterone concentrations were significantly reduced (P<0.05) concomitant with the increasing doses of acrylamide; however, 17β-estradiol (E2) concentrations were unchanged with treatment. Measurement of NOS activities indicated that total NOS (TNOS), iNOS and eNOS activities were significantly increased (P<0.05) with increasing doses of acrylamide. The results from in vitro study indicated that acrylamide reduced the viability of mouse granulosa cells in a dose-dependent manner. In summary, acrylamide affected bodily growth and development, as well as reproductive organs, the number of corpora lutea and progesterone production in female mice, possibly acting through the NOS signaling pathway.
authors.affiliation: Laboratory of Animal Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Quanwei
authors.initials: Q
authors.lastname: Wei
authors.affiliation: Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.affiliation: Laboratory of Animal Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Xingmei
authors.initials: X
authors.lastname: Li
authors.affiliation: Laboratory of Animal Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Laboratory of Animal Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: fxshi@njau.edu.cn.
authors.forename: Fangxiong
authors.initials: F
authors.lastname: Shi
mesh_terms: D020106:Acrylamide / Q000633:toxicity*; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003338:Corpus Luteum / Q000187:drug effects; D004958:Estradiol / Q000097:blood; D005260:Female; D008297:Male; D051379:Mice; D019001:Nitric Oxide Synthase / Q000378:metabolism; D009929:Organ Size / Q000187:drug effects; D011247:Pregnancy; D011374:Progesterone / Q000097:blood; D012098:Reproduction / Q000187:drug effects*; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D020106:Acrylamide; D011374:Progesterone; D004958:Estradiol; D019001:Nitric Oxide Synthase
keywords: Acrylamide; Nitric oxide synthase; Ovary; Reproductive toxicity