title: Prolonged Laccase Production by a Cold and pH Tolerant Strain of Penicillium pinophilum (MCC 1049) Isolated from a Low Temperature Environment.
abstract: Production of laccase by a cold and pH tolerant strain of Penicillium pinophilum has been investigated under different cultural conditions for up to 35 days of incubation. The fungus was originally isolated from a low temperature environment under mountain ecosystem of Indian Himalaya. The estimations were conducted at 3 temperatures (15, 25, and 35°C), a range of pH (3.5-11.5), and in presence of supplements including carbon and nitrogen sources, vitamins, and antibiotics. Optimum production of laccase was recorded at 25°C (optimum temperature for fungal growth) and 7.5 pH. The production of enzyme was recorded maximum on day 28 (11.6 ± 0.52 U/L) following a slow decline at day 35 of incubation (10.6 ± 0.80 U/L). Fructose and potassium nitrate (0.2%) among nutritional supplements, chloramphenicol (0.1%) among antibiotics, and folic acid (0.1%) among vitamins were found to be the best enhancers for production of laccase. Relatively lower but consistent production of laccase for a longer period is likely to be an ecologically important phenomenon under low temperature environment. Further, enhancement in production of enzyme using various supplements will be useful for its use in specific biotechnological applications.
authors.affiliation: Biotechnological Applications, G. B. Pant Institute of Himalayan Environment and Development, Kosi-Katarmal, Almora, Uttarakhand 263 643, India.
authors.forename: Kusum
authors.identifier: 0000-0002-3189-951X
authors.initials: K
authors.lastname: Dhakar
authors.affiliation: Biotechnological Applications, G. B. Pant Institute of Himalayan Environment and Development, Kosi-Katarmal, Almora, Uttarakhand 263 643, India.
authors.forename: Rahul
authors.initials: R
authors.lastname: Jain
authors.affiliation: Department of Biotechnology, Bhimtal Campus, Kumaun University, Nainital, Uttarakhand 263 136, India.
authors.forename: Sushma
authors.initials: S
authors.lastname: Tamta
authors.affiliation: Biotechnological Applications, G. B. Pant Institute of Himalayan Environment and Development, Kosi-Katarmal, Almora, Uttarakhand 263 643, India.
authors.forename: Anita
authors.identifier: 0000-0003-0592-0220
authors.initials: A
authors.lastname: Pandey
references.citation: Bioresour Technol. 2005 Aug;96(12):1415-8
references.pmid: 15792590
references.citation: Enzyme Res. 2013;2013:869062
references.pmid: 23710343
references.citation: J Basic Microbiol. 2009 Oct;49(5):433-40
references.pmid: 19322835
references.citation: Enzyme Res. 2011;2011:217861
references.pmid: 21755038
references.citation: World J Microbiol Biotechnol. 2014 Apr;30(4):1315-24
references.pmid: 24233773
references.citation: Curr Microbiol. 2009 Nov;59(5):502-8
references.pmid: 19688382
references.citation: Folia Microbiol (Praha). 2007;52(5):498-502
references.pmid: 18298047
references.citation: J Basic Microbiol. 2002;42(5):295-301
references.pmid: 12362400
references.citation: Microbiol Res. 2001;156(4):377-82
references.pmid: 11770856
references.citation: Int J Environ Res Public Health. 2009 Apr;6(4):1393-417
references.pmid: 19440525
references.citation: Pol J Microbiol. 2006;55(4):309-19
references.pmid: 17416068
references.citation: Annu Rev Microbiol. 1987;41:465-505
references.pmid: 3318677
references.citation: J Microbiol. 2005 Dec;43(6):555-60
references.pmid: 16410773