title: The utilization of oropharyngeal intratracheal PAMP administration and bronchoalveolar lavage to evaluate the host immune response in mice.
abstract: The host immune response to pathogens is a complex biological process. The majority of in vivo studies classically employed to characterize host-pathogen interactions take advantage of intraperitoneal injections of select bacteria or pathogen associated molecular patterns (PAMPs) in mice. While these techniques have yielded tremendous data associated with infectious disease pathobiology, intraperitoneal injection models are not always appropriate for host-pathogen interaction studies in the lung. Utilizing an acute lung inflammation model in mice, it is possible to conduct a high resolution analysis of the host innate immune response utilizing lipopolysaccharide (LPS). Here, we describe the methods to administer LPS using nonsurgical oropharyngeal intratracheal administration, monitor clinical parameters associated with disease pathogenesis, and utilize bronchoalveolar lavage fluid to evaluate the host immune response. The techniques that are described are widely applicable for studying the host innate immune response to a diverse range of PAMPs and pathogens. Likewise, with minor modifications, these techniques can also be applied in studies evaluating allergic airway inflammation and in pharmacological applications.
authors.affiliation: Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University; icallen@vt.edu.
authors.forename: Irving C
authors.initials: IC
authors.lastname: Allen
mesh_terms: D000818:Animals; D018893:Bronchoalveolar Lavage / Q000379:methods*; D054884:Host-Pathogen Interactions; D007442:Intubation, Intratracheal / Q000379:methods; D008070:Lipopolysaccharides / Q000008:administration & dosage*; D008168:Lung / Q000187:drug effects / Q000276:immunology; D008297:Male; D051379:Mice; D011014:Pneumonia / Q000139:chemically induced / Q000276:immunology* / Q000382:microbiology
chemical_list: D008070:Lipopolysaccharides
references.citation: Am J Physiol Lung Cell Mol Physiol. 2006 Nov;291(5):L1005-17
references.pmid: 16829631
references.citation: PLoS One. 2013 May 07;8(5):e63432
references.pmid: 23667616
references.citation: Front Cell Infect Microbiol. 2012 Dec 20;2:164
references.pmid: 23267442
references.citation: PLoS One. 2013 Apr 12;8(4):e62115
references.pmid: 23593509
references.citation: J Vis Exp. 2011 May 02;(51):
references.pmid: 21587154
references.citation: Am J Respir Crit Care Med. 1999 Jul;160(1):297-303
references.pmid: 10390415
references.citation: J Infect Dis. 2012 Mar 1;205(5):807-17
references.pmid: 22279123
references.citation: Am J Respir Cell Mol Biol. 1998 Dec;19(6):853-66
references.pmid: 9843919
references.citation: Am J Respir Cell Mol Biol. 2011 May;44(5):725-38
references.pmid: 21531958
references.citation: PLoS One. 2013;8(4):e60842
references.pmid: 23577168
references.citation: J Immunol. 2012 Mar 15;188(6):2884-93
references.pmid: 22323538
references.citation: J Immunol. 2009 Aug 1;183(3):2008-15
references.pmid: 19587006