title: Visualizing G protein-coupled receptors in action through confocal microscopy techniques.
abstract: G protein-coupled receptors constitute one of the most abundant entities in cellular communication. Elucidation of their structure and function as well as of their regulation began 30-40 years ago and the advance has markedly increased during the last 15 years. They participate in a plethora of cell functions such as regulation of metabolic fluxes, contraction, secretion, differentiation, or proliferation, and in essentially all activities of our organism; these receptors are targets of a large proportion of prescribed and illegal drugs. Fluorescence techniques have been used to study receptors for many years. The experimental result was usually a two-dimensional (2D) micrograph. Today, the result can be a spatiotemporal (four-dimensional, 4D) movie. Advances in microscopy, fluorescent protein design, and computer-assisted analysis have been of great importance to increase our knowledge on receptor regulation and function and create opportunities for future research. In this review we briefly depict the state of the art of the G protein-coupled receptor field and the methodologies used to study G protein-coupled receptor location, trafficking, dimerization, and other types of receptor-protein interaction. Fluorescence techniques now permit the capture of receptor images with high resolution and, together with a variety of fluorescent dyes that color organelles (such as the plasma membrane or the nucleus) or the cytoskeleton, allow researchers to obtain a much clearer idea of what is taking place at the cellular level. These developments are changing the way we explore cell communication and signal transduction, permitting deeper understanding of the physiological and pathophysiological processes.
authors.affiliation: Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, México, D.F., Mexico.
authors.forename: Jean A
authors.initials: JA
authors.lastname: Castillo-Badillo
authors.affiliation: Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, México, D.F., Mexico.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Cabrera-Wrooman
authors.affiliation: Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, México, D.F., Mexico. Electronic address: agarcia@ifc.unam.mx.
authors.forename: J Adolfo
authors.initials: JA
authors.lastname: García-Sáinz
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D006801:Humans; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology*
chemical_list: D043562:Receptors, G-Protein-Coupled
keywords: Confocal microscopy; G protein-coupled receptors; Green fluorescent protein; Hormones; Imaging