title: A mouse monoclonal antibody against Alexa Fluor 647.
abstract: Fluorophores are essential tools in molecular and cell biology. However, their application is mostly confined to the singular exploitation of their fluorescent properties. To enhance the versatility and expand the use of the fluorophore Alexa Fluor 647 (AF647), we generated a mouse monoclonal antibody against it. We demonstrate its use of AF647 for immunoblot, immunoprecipitation, and cytofluorimetry.
authors.affiliation: Whitehead Institute for Biomedical Research , Cambridge, Massachusetts.
authors.forename: Irene
authors.initials: I
authors.lastname: Wuethrich
authors.forename: Eduardo
authors.initials: E
authors.lastname: Guillen
authors.forename: Hidde L
authors.initials: HL
authors.lastname: Ploegh
mesh_terms: D000818:Animals; D058846:Antibodies, Monoclonal, Murine-Derived / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D000918:Antibody Specificity; D001427:Bacterial Toxins / Q000737:chemistry; D015153:Blotting, Western; D002232:Carbocyanines / Q000737:chemistry*; D004926:Escherichia coli; D005260:Female; D005434:Flow Cytometry; D005456:Fluorescent Dyes / Q000737:chemistry*; D006825:Hybridomas; D007074:Immunoglobulin G; D008807:Mice, Inbred BALB C; D008856:Microscopy, Fluorescence; D016253:Microscopy, Immunoelectron
chemical_list: C569686:Alexa Fluor 647; D058846:Antibodies, Monoclonal, Murine-Derived; D001427:Bacterial Toxins; D002232:Carbocyanines; D005456:Fluorescent Dyes; D007074:Immunoglobulin G