title: Two residues predominantly dictate functional difference in motility between Shewanella oneidensis flagellins FlaA and FlaB.
abstract: Nearly half of flagellated microorganisms possess a multiple-flagellin system. Although a functional filament can be formed from one of multiple flagellins alone in many bacteria, it is more common that one flagellin is the major constituent and others contribute. Underlying mechanisms proposed for such scenarios cover flagellin regulation of various levels, including transcription, translation, post-translational modification, secretion, and filament assembly. In Shewanella oneidensis, the flagellar filament is composed of FlaA and FlaB flagellins; the latter is the major one in terms of motility. In this study, we showed that regulation of all levels except for filament assembly is indistinguishable between these two flagellins. Further analyses revealed that two amino acid residues predominantly dictated functional difference with respect to motility. Given that Shewanella prefer a solid surface-associated life style, of which filaments consisting of either FlaA or FlaB are equally supportive, we envision that roles of flagella in surface adhesion and formation of bacterial communities are particularly important for their survival and proliferation in these specific niches.
authors.affiliation: From the Institute of Microbiology and College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, China and.
authors.forename: Linlin
authors.initials: L
authors.lastname: Sun
authors.affiliation: From the Institute of Microbiology and College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, China and.
authors.forename: Yangyang
authors.initials: Y
authors.lastname: Dong
authors.affiliation: From the Institute of Microbiology and College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, China and.
authors.forename: Miaomiao
authors.initials: M
authors.lastname: Shi
authors.affiliation: From the Institute of Microbiology and College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, China and.
authors.forename: Miao
authors.initials: M
authors.lastname: Jin
authors.affiliation: the Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907.
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: the Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907.
authors.forename: Zhao-Qing
authors.initials: ZQ
authors.lastname: Luo
authors.affiliation: From the Institute of Microbiology and College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, China and haichung@zju.edu.cn.
authors.forename: Haichun
authors.initials: H
authors.lastname: Gao
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D005407:Flagella / Q000378:metabolism / Q000502:physiology*; D005408:Flagellin / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D018613:Microscopy, Confocal; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009068:Movement / Q000502:physiology; D009154:Mutation; D017433:Protein Structure, Secondary; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D020592:Shewanella / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
chemical_list: D000596:Amino Acids; D005408:Flagellin; C067138:flaA protein, bacteria; C416532:flaB flagellin; D049452:Green Fluorescent Proteins
keywords: Cell Motility; Flagellin; Gene Regulation; Post-translational Modification; Protein Structure; Protein Synthesis; Shewanella
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