title: Flow-through synthesis on Teflon-patterned paper to produce peptide arrays for cell-based assays.
abstract: A simple method is described for the patterned deposition of Teflon on paper to create an integrated platform for parallel organic synthesis and cell-based assays. Solvent-repelling barriers made of Teflon-impregnated paper confine organic solvents to specific zones of the patterned array and allow for 96 parallel flow-through syntheses on paper. The confinement and flow-through mixing significantly improves the peptide yield and simplifies the automation of this synthesis. The synthesis of 100 peptides ranging from 7 to 14 amino acids in length gave over 60% purity for the majority of the peptides (>95% yield per coupling/deprotection cycle). The resulting peptide arrays were used in cell-based screening to identify 14 potent bioactive peptides that support the adhesion or proliferation of breast cancer cells in a 3D environment. In the future, this technology could be used for the screening of more complex phenotypic responses, such as cell migration or differentiation.
authors.affiliation: Department of Chemistry, University of Alberta, Edmonton, AB T6G 2G2 (Canada).
authors.forename: Frédérique
authors.initials: F
authors.lastname: Deiss
authors.forename: Wadim L
authors.initials: WL
authors.lastname: Matochko
authors.forename: Natasha
authors.initials: N
authors.lastname: Govindasamy
authors.forename: Edith Y
authors.initials: EY
authors.lastname: Lin
authors.forename: Ratmir
authors.initials: R
authors.lastname: Derda
mesh_terms: D010209:Paper; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry; D011138:Polytetrafluoroethylene / Q000737:chemistry*; D046888:Tissue Array Analysis / Q000295:instrumentation*
chemical_list: D010455:Peptides; D011138:Polytetrafluoroethylene
keywords: cell adhesion; high-throughput screening; peptide arrays; peptides; solid-phase synthesis