title: Adenosine A2B and A3 receptor location at the mouse neuromuscular junction.
abstract: To date, four subtypes of adenosine receptors have been cloned (A(1)R, A(2A)R, A(2B)R, and A(3)R). In a previous study we used confocal immunocytochemistry to identify A(1)R and A(2A)R receptors at mouse neuromuscular junctions (NMJs). The data shows that these receptors are localized differently in the three cells (muscle, nerve and glia) that configure the NMJs. A(1)R localizes in the terminal teloglial Schwann cell and nerve terminal, whereas A(2A)R localizes in the postsynaptic muscle and in the axon and nerve terminal. Here, we use Western blotting to investigate the presence of A(2B)R and A(3)R receptors in striated muscle and immunohistochemistry to localize them in the three cells of the adult neuromuscular synapse. The data show that A(2B)R and A(3)R receptors are present in the nerve terminal and muscle cells at the NMJs. Neither A(2B)R nor A(3)R receptors are localized in the Schwann cells. Thus, the four subtypes of adenosine receptors are present in the motor endings. The presence of these receptors in the neuromuscular synapse allows the receptors to be involved in the modulation of transmitter release.
authors.affiliation: Unitat d'Histologia i Neurobiologia (UHN), Facultat de Medicina i Ciències de la Salut, Universitat Rovira i Virgili, Reus, Spain.
authors.forename: Neus
authors.initials: N
authors.lastname: Garcia
authors.forename: Mercedes
authors.initials: M
authors.lastname: Priego
authors.forename: Erica
authors.initials: E
authors.lastname: Hurtado
authors.forename: Teresa
authors.initials: T
authors.lastname: Obis
authors.forename: Manel M
authors.initials: MM
authors.lastname: Santafe
authors.forename: Marta
authors.initials: M
authors.lastname: Tomàs
authors.forename: Maria Angel
authors.initials: MA
authors.lastname: Lanuza
authors.forename: Josep
authors.initials: J
authors.lastname: Tomàs
mesh_terms: D000818:Animals; D015153:Blotting, Western; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D032342:Muscle Cells / Q000737:chemistry; D009457:Neuroglia / Q000737:chemistry; D009469:Neuromuscular Junction / Q000737:chemistry*; D009474:Neurons / Q000737:chemistry; D043704:Receptor, Adenosine A2B / Q000032:analysis*; D043684:Receptor, Adenosine A3 / Q000032:analysis*
chemical_list: D043704:Receptor, Adenosine A2B; D043684:Receptor, Adenosine A3
keywords: adenosine receptors; cholinergic synapses; immunofluorescence; motor end-plate; motor nerve terminal
references.citation: J Comp Neurol. 2001 Oct 8;439(1):46-64
references.pmid: 11579381
references.citation: Br J Pharmacol. 2013 Aug;169(8):1810-23
references.pmid: 23731236
references.citation: Eur J Neurosci. 2013 Jul;38(2):2229-41
references.pmid: 23607735
references.citation: Br J Pharmacol. 1973 Dec;49(4):724-5
references.pmid: 4788046
references.citation: Prog Neurobiol. 2002 Jun;67(3):203-33
references.pmid: 12169297
references.citation: Pflugers Arch. 1993 Sep;424(5-6):503-10
references.pmid: 8255734
references.citation: Acta Physiol Scand. 2000 Aug;169(4):283-90
references.pmid: 10951119
references.citation: Circ Res. 2000 Jan 7-21;86(1):76-85
references.pmid: 10625308
references.citation: Hypertension. 1999 Jan;33(1 Pt 2):511-6
references.pmid: 9931157
references.citation: Mol Pharmacol. 1995 Jun;47(6):1126-32
references.pmid: 7603451
references.citation: Am J Physiol Renal Physiol. 2002 Jul;283(1):F41-51
references.pmid: 12060585
references.citation: Neuroreport. 2003 Aug 26;14(12):1645-8
references.pmid: 14502093
references.citation: Neuroscience. 2006 Sep 29;142(1):71-85
references.pmid: 16843602
references.citation: Nature. 1970 Aug 15;227(5259):680-5
references.pmid: 5432063
references.citation: Br J Pharmacol. 1991 Jun;103(2):1614-20
references.pmid: 1679362
references.citation: Biochem Biophys Res Commun. 2003 Jul 18;307(1):180-7
references.pmid: 12849998
references.citation: J Neurochem. 2003 Jul;86(1):220-7
references.pmid: 12807441
references.citation: Physiol Res. 2010;59(5):803-810
references.pmid: 21142401
references.citation: Pharmacol Rev. 1998 Sep;50(3):413-92
references.pmid: 9755289
references.citation: Br J Pharmacol. 2005 Aug;145(7):954-62
references.pmid: 15880138
references.citation: Synapse. 2005 Sep 15;57(4):229-34
references.pmid: 15986392
references.citation: Basic Res Cardiol. 2011 May;106(3):385-96
references.pmid: 21246204
references.citation: Am J Physiol Cell Physiol. 2006 Aug;291(2):C254-65
references.pmid: 16571869
references.citation: J Endocrinol. 2006 Oct;191(1):147-57
references.pmid: 17065398
references.citation: Genes Dev. 2000 Dec 1;14(23):2919-37
references.pmid: 11114882
references.citation: Int Anesthesiol Clin. 2006 Spring;44(2):21-76
references.pmid: 16849956
references.citation: Prog Brain Res. 1996;109:231-41
references.pmid: 9009712
references.citation: Brain Res. 2000 Sep 22;877(2):245-50
references.pmid: 10986338
references.citation: Br J Pharmacol. 1996 Jul;118(6):1461-8
references.pmid: 8832073
references.citation: Eur J Neurosci. 2003 Jan;17(1):119-27
references.pmid: 12534975
references.citation: Neuroscience. 2004;123(1):61-73
references.pmid: 14667442
references.citation: Physiol Behav. 2007 Sep 10;92(1-2):15-20
references.pmid: 17574632
references.citation: J Pharmacol Exp Ther. 2010 Jan;332(1):153-63
references.pmid: 19789361
references.citation: Eur J Neurosci. 2006 Jul;24(1):105-15
references.pmid: 16800865
references.citation: Neuroscience. 2005;132(4):893-903
references.pmid: 15857695