title: Characterization of antioxidant polysaccharides from Auricularia auricular using microwave-assisted extraction.
abstract: Influence of microwave-assisted extraction (MAE) to the characterization and corresponding antioxidant activity of polysaccharides from Auricularia auricula (AAP) was determined. According to gas chromatography-mass spectrometry, high performance size exclusion chromatography, Fourier transform infrared spectroscopy, partial acid hydrolysis, periodic acid oxidation, Smith degradation, methylation analysis and atomic force microscopy, AAP was a heteropolysaccharide (composed of glucose, galactose, mannose, arabinose and rhamnose at the molar ratio of 37.53:1:4.32:0.93:0.91) with the molecular weight of 2.77×10(4) Da, observed as a spherical lump, and the backbone of AAP was mainly composed by glucose with (1→3) linked. Moreover, AAP exhibited significant in vitro antioxidant activity in scavenging free radicals (ABTS, DPPH, superoxide and hydroxyl radicals), in lipid peroxidation and reducing power assays. The present result suggested that AAP by MAE had low molecular weight and remarkable antioxidant capability.
authors.affiliation: College of Light Industry, Textile and Food Engineering, Sichuan University, Chengdu 610065, PR China; National Engineering Laboratory of Clean Technology for Leather Manufacture, Sichuan University, Chengdu 610065, PR China.
authors.forename: Wei-Cai
authors.initials: WC
authors.lastname: Zeng
authors.affiliation: College of Life Sciences, Sichuan University, Chengdu 610064, PR China.
authors.forename: Zeng
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: College of Light Industry, Textile and Food Engineering, Sichuan University, Chengdu 610065, PR China.
authors.forename: Hong
authors.initials: H
authors.lastname: Gao
authors.affiliation: College of Light Industry, Textile and Food Engineering, Sichuan University, Chengdu 610065, PR China.
authors.forename: Li-Rong
authors.initials: LR
authors.lastname: Jia
authors.affiliation: National Engineering Laboratory of Clean Technology for Leather Manufacture, Sichuan University, Chengdu 610065, PR China.
authors.forename: Wu-Yong
authors.initials: WY
authors.lastname: Chen
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D001487:Basidiomycota; D052160:Benzothiazoles / Q000737:chemistry; D001713:Biphenyl Compounds / Q000737:chemistry; D062610:Fungal Polysaccharides / Q000737:chemistry*; D006868:Hydrolysis; D017665:Hydroxyl Radical / Q000737:chemistry; D008745:Methylation; D008872:Microwaves; D010084:Oxidation-Reduction; D010504:Periodic Acid / Q000737:chemistry; D010851:Picrates / Q000737:chemistry; D013451:Sulfonic Acids / Q000737:chemistry; D013481:Superoxides / Q000737:chemistry
chemical_list: D000975:Antioxidants; D052160:Benzothiazoles; D001713:Biphenyl Compounds; D062610:Fungal Polysaccharides; D010851:Picrates; D013451:Sulfonic Acids; D010504:Periodic Acid; D013481:Superoxides; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D017665:Hydroxyl Radical; C009288:metaperiodate; C004931:1,1-diphenyl-2-picrylhydrazyl