title: Evaluation of Toll-like, chemokine, and integrin receptors on monocytes and neutrophils from peripheral blood of septic patients and their correlation with clinical outcomes.
abstract: Recognition of pathogens is performed by specific receptors in cells of the innate immune system, which may undergo modulation during the continuum of clinical manifestations of sepsis. Monocytes and neutrophils play a key role in host defense by sensing and destroying microorganisms. This study aimed to evaluate the expression of CD14 receptors on monocytes; CD66b and CXCR2 receptors on neutrophils; and TLR2, TLR4, TLR5, TLR9, and CD11b receptors on both cell types of septic patients. Seventy-seven septic patients (SP) and 40 healthy volunteers (HV) were included in the study, and blood samples were collected on day zero (D0) and after 7 days of therapy (D7). Evaluation of the cellular receptors was carried out by flow cytometry. Expression of CD14 on monocytes and of CD11b and CXCR2 on neutrophils from SP was lower than that from HV. Conversely, expression of TLR5 on monocytes and neutrophils was higher in SP compared with HV. Expression of TLR2 on the surface of neutrophils and that of TLR5 on monocytes and neutrophils of SP was lower at D7 than at D0. In addition, SP who survived showed reduced expression of TLR2 and TLR4 on the surface of neutrophils at D7 compared to D0. Expression of CXCR2 for surviving patients was higher at follow-up compared to baseline. We conclude that expression of recognition and cell signaling receptors is differentially regulated between SP and HV depending on the receptor being evaluated.
authors.affiliation: Disciplina de Infectologia, Departamento de Medicina, Hospital São Paulo, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brasil.
authors.forename: S C
authors.initials: SC
authors.lastname: Silva
authors.affiliation: Disciplina de Infectologia, Departamento de Medicina, Hospital São Paulo, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brasil.
authors.forename: G L
authors.initials: GL
authors.lastname: Baggio-Zappia
authors.affiliation: Disciplina de Infectologia, Departamento de Medicina, Hospital São Paulo, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brasil.
authors.forename: M K C
authors.initials: MK
authors.lastname: Brunialti
authors.affiliation: Unidade de Terapia Intensiva, Hospital Israelita Albert Einstein, São Paulo, SP, Brasil.
authors.forename: M S C
authors.initials: MS
authors.lastname: Assunçao
authors.affiliation: Unidade de Terapia Intensiva, Hospital Sírio Libanês, São Paulo, SP, Brasil.
authors.forename: L C P
authors.initials: LC
authors.lastname: Azevedo
authors.affiliation: Disciplina de Anestesiologia, Departamento de Cirurgia, Hospital São Paulo, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brasil.
authors.forename: F R
authors.initials: FR
authors.lastname: Machado
authors.affiliation: Disciplina de Infectologia, Departamento de Medicina, Hospital São Paulo, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brasil.
authors.forename: R
authors.initials: R
authors.lastname: Salomao
mesh_terms: D000328:Adult; D000368:Aged; D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D015703:Antigens, CD / Q000097:blood; D039481:CD11b Antigen / Q000097:blood; D015815:Cell Adhesion Molecules / Q000097:blood; D018925:Chemokines / Q000097:blood*; D002675:Child, Preschool; D005260:Female; D005434:Flow Cytometry; D058851:GPI-Linked Proteins / Q000097:blood; D017052:Hospital Mortality; D006801:Humans; D016130:Immunophenotyping; D016023:Integrins / Q000097:blood*; D007362:Intensive Care Units; D018950:Lipopolysaccharide Receptors / Q000097:blood; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000737:chemistry*; D009504:Neutrophils / Q000737:chemistry*; D023063:Receptors, Interleukin-8B / Q000097:blood; D018805:Sepsis / Q000276:immunology* / Q000628:therapy; D018709:Statistics, Nonparametric; D051195:Toll-Like Receptor 2 / Q000097:blood; D051197:Toll-Like Receptor 4 / Q000097:blood; D051198:Toll-Like Receptor 5 / Q000097:blood; D051217:Toll-Like Receptor 9 / Q000097:blood; D051193:Toll-Like Receptors / Q000097:blood*; D016896:Treatment Outcome
chemical_list: D000900:Anti-Bacterial Agents; D015703:Antigens, CD; D039481:CD11b Antigen; C504120:CEACAM8 protein, human; D015815:Cell Adhesion Molecules; D018925:Chemokines; D058851:GPI-Linked Proteins; D016023:Integrins; D018950:Lipopolysaccharide Receptors; D023063:Receptors, Interleukin-8B; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4; D051198:Toll-Like Receptor 5; D051217:Toll-Like Receptor 9; D051193:Toll-Like Receptors
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