title: Investigation of the peptide adsorption on ZrO2, TiZr, and TiO2 surfaces as a method for surface modification.
abstract: Specific surface binding peptides offer a versatile and interesting possibility for the development of biocompatible implant materials. Therefore, eight peptide sequences were examined in regard to their adsorption on zirconium oxide (ZrO2), titanium zircon (TiZr), and titanium (c.p. Ti). Surface plasmon resonance (SPR) measurements were performed on Ti coated sensor chips to determine the kinetics of the interactions and kinetic rate constants (kon, koff, KD, and Rmax). We also investigated the interactions which are present in our system. Electrostatic and coordinative interactions were found to play a major role in the adsorption process. Four of the eight examined peptide sequences showed a significant adsorption on all investigated materials. Moreover, the two peptides with the highest adsorption could be quantified (up to 370 pmol/cm(2)). For potential biomaterials applications, we proved the stability of the adsorption of selected peptides in cell culture media, under competition with proteins and at body temperature (37 °C), and their biocompatibility via their effects on the adhesion and proliferation of human mesenchymal stem cells (hMSCs). The results qualify the peptides as anchor peptides for the biofunctionalization of implants.
authors.affiliation: Lehrstuhl für Allgemeine Biochemie, Technische Universität Dresden , Bergstr. 66, Dresden, Saxony 01069, Germany.
authors.forename: Tina
authors.initials: T
authors.lastname: Micksch
authors.forename: Nora
authors.initials: N
authors.lastname: Liebelt
authors.forename: Dieter
authors.initials: D
authors.lastname: Scharnweber
authors.forename: Bernd
authors.initials: B
authors.lastname: Schwenzer
mesh_terms: D000327:Adsorption; D000595:Amino Acid Sequence; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D059630:Mesenchymal Stem Cells / Q000166:cytology; D008969:Molecular Sequence Data; D009994:Osmolar Concentration; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism; D055672:Static Electricity; D020349:Surface Plasmon Resonance; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*; D015040:Zirconium / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D010455:Peptides; C009495:titanium dioxide; D015040:Zirconium; D014025:Titanium; C028541:zirconium oxide