title: Altered vimentin protein expression in human dermal microvascular endothelial cells after ultraviolet or intense pulsed light treatment.
abstract: BACKGROUND
  Endothelial cell senescence is closely related to tissue aging and age-related vascular disease. Detailed pathophysiology and essential biomarkers of skin aging are not well known. A recent report suggests that advanced glycosylation end products, especially N(ϵ) -(carboxymethyl)lysine (CML) modification of vimentin, accelerate the aging process.
  
  
  OBJECTIVE
  To identify protein biomarkers of aging in skin.
  
  
  METHODS
  Proteomics analysis was performed to identify differentially expressed proteins in human dermal microvascular endothelial cells (HDMEC) treated with ultraviolet (UV) or intense pulsed light (IPL). Proteome maps of UV-treated, IPL-treated, and untreated HDMEC were constructed, with identification of altered protein spots by matrix-assisted laser desorption/ionization mass spectrometry. Differential expression and glycation modification of vimentin were found by this approach and further examined by fluorescence-activated cell sorting.
  
  
  RESULTS
  Twenty-two differentially expressed protein spots were identified. Among them, vimentin was specifically up-regulated in UV-treated HDMECs. On the other hand, it was down-regulated after IPL. Increased expression of CML-vimentin in HDMEC during culture (Passage 6 vs. 12) was noted, and this effect was reversed by IPL treatment.
  
  
  CONCLUSIONS
  Vimentin and CML should be useful markers for cell senescence, as well as for evaluating the level of aging. Also, targeting increased vimentin expression and its advanced glycation end products could present a target for the treatment of skin aging.
authors.affiliation: Department of Dermatology and Cutaneous Biology Research Institute, Institute for Human Tissue Restoration, Yonsei University College of Medicine, Seoul, South Korea.
authors.forename: Jung U
authors.initials: JU
authors.lastname: Shin
authors.forename: Won Jai
authors.initials: WJ
authors.lastname: Lee
authors.forename: Sang Ho
authors.initials: SH
authors.lastname: Oh
authors.forename: Do Young
authors.initials: DY
authors.lastname: Kim
authors.forename: Dae Suk
authors.initials: DS
authors.lastname: Kim
authors.forename: Inhee
authors.initials: I
authors.lastname: Jung
authors.forename: Ju Hee
authors.initials: JH
authors.lastname: Lee
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000378:metabolism / Q000528:radiation effects*; D006801:Humans; D055806:Microvessels / Q000166:cytology; D040901:Proteomics; D015595:Skin Aging / Q000502:physiology* / Q000528:radiation effects; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014466:Ultraviolet Rays; D015854:Up-Regulation; D014746:Vimentin / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D014746:Vimentin
keywords: HDMEC; proteomics; skin aging; vimentin