title: Dietary sugar in healthy female primates perturbs oocyte maturation and in vitro preimplantation embryo development.
abstract: The consumption of refined sugars continues to pose a significant health risk. However, nearly nothing is known about the effects of sugar intake by healthy women on the oocyte or embryo. Using rhesus monkeys, we show that low-dose sucrose intake over a 6-month period has an impact on the oocyte with subsequent effects on the early embryo. The ability of oocytes to resume meiosis was significantly impaired, although the differentiation of the somatic component of the ovarian follicle into progesterone-producing cells was not altered. Although the small subset of oocytes that did mature were able to be fertilized in vitro and develop into preimplantation blastocysts, there were >1100 changes in blastocyst gene expression. Because sucrose treatment ended before fertilization, the effects of sugar intake by healthy primates are concluded to be epigenetic modifications to the immature oocyte that are manifest in the preimplantation embryo.
authors.affiliation: Department of Obstetrics, Gynecology, and Reproductive Sciences (C.L.C.), University of Maryland School of Medicine, Baltimore, Maryland 21210; Department of Animal Science (K.E.L., U.M.), Michigan State University, East Lansing, Michigan 48824; The Fels Institute for Cancer Research and Molecular Biology and Department of Biochemistry (N.R.M.), Temple University School of Medicine, Philadelphia, Pennsylvania 19140; and California National Primate Research Center and Department of Obstetrics and Gynecology (C.A.V.), University of California, Davis, California 95616.
authors.forename: Charles L
authors.initials: CL
authors.lastname: Chaffin
authors.forename: Keith E
authors.initials: KE
authors.lastname: Latham
authors.forename: Namdori R
authors.initials: NR
authors.lastname: Mtango
authors.forename: Uros
authors.initials: U
authors.lastname: Midic
authors.forename: Catherine A
authors.initials: CA
authors.lastname: VandeVoort
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000187:drug effects / Q000378:metabolism; D002478:Cells, Cultured; D019422:Dietary Sucrose / Q000008:administration & dosage*; D047108:Embryonic Development / Q000187:drug effects*; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D008253:Macaca mulatta; D008540:Meiosis / Q000187:drug effects / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D009865:Oocytes / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D006080:Ovarian Follicle / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D011247:Pregnancy; D020133:Reverse Transcriptase Polymerase Chain Reaction; D059467:Transcriptome / Q000187:drug effects* / Q000235:genetics
chemical_list: D019422:Dietary Sucrose
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