title: pH- and glucose-sensitive glycopolymer nanoparticles based on phenylboronic acid for triggered release of insulin.
abstract: Amphiphilic poly(acrylic acid-co-acrylamidophenylboronic acid)-block-poly(2-acryloxyethyl galactose)-block-poly(acrylic acid-co-acrylamidophenylboronic acid) (((PAA-co-PAAPBA)-b-)₂PAEG) copolymer was fabricated: The poly(2-acryloyloxyethyl pentaacetylgalactoside) (PAEAcG) with narrow molecular weight distributions (Mw/Mn≤1.22) was prepared by atom transfer radical polymerization (ATRP) using dibromo-p-xylene (DBX) as initiator. Then the well-defined triblock copolymer poly(t-butyl acrylate)-b-poly(2-acryloyloxyethyl pentaacetylgalactoside)-b-poly(t-butyl acrylate) (PtBA-b-PAEAcG-b-PtBA) was synthesized by ATRP of tBA using PAEAcG homopolymer with dibromo end groups as macroinitiator. After hydrolysis of t-butyl acrylate block, amide linkage and deacetylation, the final copolymer ((PAA-co-PAAPBA)-b-)₂PAEG was obtained. Because of characteristics of three different segments, amphiphilic ((PAA-co-PAAPBA)-b-)₂PAEG can self-assemble into pH- and glucose-responsive nanoparticles studied by dynamic light scattering (DLS) and transmission electron microscopy (TEM). Furthermore, the in vitro release profiles of insulin also revealed obvious pH- and glucose-sensitivity of the nanoparticles. The analysis of cell viability suggested that the copolymer nanoparticles had good cytocompatibility.
authors.affiliation: Key Laboratory of Functional Polymer Materials Ministry of Education, Institute of Polymer Chemistry, Nankai University, 94# Weijin Road, Tianjin 300071, China.
authors.forename: Yanxia
authors.initials: Y
authors.lastname: Wang
authors.forename: Xinge
authors.initials: X
authors.lastname: Zhang
authors.forename: Yucai
authors.initials: Y
authors.lastname: Han
authors.forename: Cui
authors.initials: C
authors.lastname: Cheng
authors.forename: Chaoxing
authors.initials: C
authors.lastname: Li
mesh_terms: D000818:Animals; D001897:Boronic Acids / Q000737:chemistry; D016466:CHO Cells; D002470:Cell Survival / Q000187:drug effects; D003412:Cricetulus; D065546:Drug Liberation; D005947:Glucose / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007328:Insulin / Q000737:chemistry*; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D011108:Polymers / Q000008:administration & dosage / Q000737:chemistry*
chemical_list: D001897:Boronic Acids; D007328:Insulin; D011108:Polymers; D005947:Glucose; C010686:benzeneboronic acid