title: Heparanase induces inflammatory cell recruitment in vivo by promoting adhesion to vascular endothelium.
abstract: Heparanase (HPSE1) is known to be involved in mechanisms of metastatic tumor cell migration. This enzyme selectively cleaves heparan sulfate proteoglycans (HSPG), which are ubiquitously expressed in mammals and are known to be involved in regulating the activity of an array of inflammatory mediators. In the present study, we have investigated the effects of human recombinant heparanase, the inactive precursor of this enzyme (proheparanase) and enzymatically inactivated heparanase, on inflammatory cell recruitment in the rat and on human leukocyte-endothelial adhesion in vitro. Intraperitoneal injection of heparanase (500 μg) induced a significant inflammatory cell infiltrate in the rat, as assessed by peritoneal lavage 4 h later. Intravital microscopy of the mesenteric microcirculation of anesthetized rats showed an increase in rolling and adherent cells in postcapillary venules that was sensitive to heparin, a nonselective inhibitor of heparanase activity. In vitro, heparanase augmented the adhesion of human neutrophils and mononuclear cells to human umbilical vein endothelial cells in a concentration-dependent manner. Proheparanase had similar effects to the active enzyme both with respect to leukocyte accumulation in the peritoneal cavity and adhesion in vitro. However, heat-inactivated heparanase induced cell adhesion in vitro but was without effect in vivo. Together, these data indicate a role for heparanase in inflammatory cell trafficking in vivo that appears to require enzymatic activity.
authors.affiliation: Department of Pharmacology, University College London School of Pharmacy, London, United Kingdom; rebecca.lever@ucl.ac.uk.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Lever
authors.affiliation: Sackler Institute of Pulmonary Pharmacology, King's College London, London, United Kingdom; and.
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Rose
authors.affiliation: Manchester Institute of Biotechnology, Faculty of Life Sciences, University of Manchester, Manchester, United Kingdom.
authors.forename: Edward A
authors.initials: EA
authors.lastname: McKenzie
authors.affiliation: Sackler Institute of Pulmonary Pharmacology, King's College London, London, United Kingdom; and.
authors.forename: Clive P
authors.initials: CP
authors.lastname: Page
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000235:genetics; D002465:Cell Movement / Q000235:genetics; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000201:enzymology* / Q000378:metabolism; D005966:Glucuronidase / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007249:Inflammation / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D007962:Leukocytes / Q000166:cytology* / Q000201:enzymology; D051381:Rats; D000099175:Heparanase
chemical_list: D005966:Glucuronidase; D000099175:Heparanase
keywords: cell trafficking; heparan sulfate; inflammation; mononuclear leukocyte; neutrophil