title: T-cell epitope discovery technologies.
abstract: Despite tremendous potential utility in clinical medicine and research, the discovery and characterization of T-cell antigens has lagged behind most other areas of health research in joining the high-throughput '-omics' revolution. Partially responsible for this is the complex nature of the interactions between effector T cells and antigen-presenting cells. Further contributing to the challenge is the vastness of both the T-cell repertoire and the large number of potential T-cell epitopes. In this review, we trace the development of various discovery strategies, the technical platforms used to carry them out, and we assess the level of success achieved in the field today.
authors.affiliation: Michael Smith Genome Sciences Centre, BC Cancer Agency, 675 W 10th Avenue, Vancouver, British Columbia V5Z 1L3, Canada; Department of Medical Genetics, University of British Columbia, C201 - 4500 Oak Street, Vancouver, British Columbia V6H 3N1, Canada. Electronic address: gsharma@bsgsc.ca.
authors.forename: Govinda
authors.initials: G
authors.lastname: Sharma
authors.affiliation: Michael Smith Genome Sciences Centre, BC Cancer Agency, 675 W 10th Avenue, Vancouver, British Columbia V5Z 1L3, Canada; Department of Medical Genetics, University of British Columbia, C201 - 4500 Oak Street, Vancouver, British Columbia V6H 3N1, Canada; Department of Molecular Biology and Biochemistry, Simon Fraser University, 8888 University Drive, Burnaby, British Columbia V5A 1S6, Canada. Electronic address: rholt@bcgsc.ca.
authors.forename: Robert A
authors.initials: RA
authors.lastname: Holt
mesh_terms: D017951:Antigen Presentation; D000938:Antigen-Presenting Cells / Q000737:chemistry* / Q000276:immunology; D018984:Epitopes, T-Lymphocyte / Q000737:chemistry* / Q000276:immunology; D015723:Gene Library; D057166:High-Throughput Screening Assays; D006801:Humans; D008285:Major Histocompatibility Complex / Q000276:immunology; D046228:Microarray Analysis; D010455:Peptides / Q000737:chemistry* / Q000276:immunology; D011948:Receptors, Antigen, T-Cell / Q000737:chemistry* / Q000276:immunology; D013601:T-Lymphocytes / Q000737:chemistry* / Q000276:immunology
chemical_list: D018984:Epitopes, T-Lymphocyte; D010455:Peptides; D011948:Receptors, Antigen, T-Cell
keywords: Epitopes; High-throughput screening; Major histocompatibility complex; T-cell receptor