title: Proteomics analysis of the peritoneal dialysate effluent reveals the presence of calcium-regulation proteins and acute inflammatory response.
abstract: BACKGROUND
  Peritoneal dialysis (PD) is a form of renal replacement used for advanced chronic kidney disease. PD effluent holds a great potential for biomarker discovery for diagnosis and prognosis. In this study a novel approach to unravelling the proteome of PD effluent based-on dithiothreitol depletion followed by 2D-SDS-PAGE and protein identification using tandem mass spectrometry is proposed.
  
  
  RESULTS
  A total of 49 spots were analysed revealing 25 proteins differentially expressed, among them many proteins involved in calcium regulation.
  
  
  CONCLUSIONS
  Remarkably, a group of proteins dealing with calcium metabolism and calcium regulation has been found to be lost through peritoneal dialysate effluent, giving thus a potential explanation to the calcification of soft tissues in patients subjected to peritoneal dialysis and kidney injury. Comparison of literature dealing with PD is difficult due to differences in sample treatment and analytical methodologies.
authors.affiliation: BIOSCOPE Research Group. REQUIMTE, Departamento de Química. Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, Caparica, 2829-516, Portugal ; PROTEOMASS Scientific Society, Madan Parque, Rua dos Inventores, Caparica, 2825-182, Portugal.
authors.forename: Elisabete
authors.initials: E
authors.lastname: Oliveira
authors.affiliation: BIOSCOPE Research Group. REQUIMTE, Departamento de Química. Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, Caparica, 2829-516, Portugal ; PROTEOMASS Scientific Society, Madan Parque, Rua dos Inventores, Caparica, 2825-182, Portugal.
authors.forename: José E
authors.initials: JE
authors.lastname: Araújo
authors.affiliation: SING Group. Informatics Department. Higher Technical School of Computer Engineering, University of Vigo, Ourense, Spain.
authors.forename: Silvana
authors.initials: S
authors.lastname: Gómez-Meire
authors.affiliation: BIOSCOPE Research Group. REQUIMTE, Departamento de Química. Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, Caparica, 2829-516, Portugal ; PROTEOMASS Scientific Society, Madan Parque, Rua dos Inventores, Caparica, 2825-182, Portugal.
authors.forename: Carlos
authors.initials: C
authors.lastname: Lodeiro
authors.affiliation: Servicio de Nefrología, Complejo Hospitalario Universitario de Ourense, Ourense, 32004, España.
authors.forename: Cristina
authors.initials: C
authors.lastname: Perez-Melon
authors.affiliation: Servicio de Nefrología, Complejo Hospitalario Universitario de Ourense, Ourense, 32004, España.
authors.forename: Elena
authors.initials: E
authors.lastname: Iglesias-Lamas
authors.affiliation: Servicio de Nefrología, Complejo Hospitalario Universitario de Ourense, Ourense, 32004, España.
authors.forename: Alfonso
authors.initials: A
authors.lastname: Otero-Glez
authors.affiliation: BIOSCOPE Research Group. REQUIMTE, Departamento de Química. Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, Caparica, 2829-516, Portugal ; PROTEOMASS Scientific Society, Madan Parque, Rua dos Inventores, Caparica, 2825-182, Portugal.
authors.forename: José L
authors.initials: JL
authors.lastname: Capelo
authors.affiliation: BIOSCOPE Research Group. REQUIMTE, Departamento de Química. Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, Caparica, 2829-516, Portugal ; PROTEOMASS Scientific Society, Madan Parque, Rua dos Inventores, Caparica, 2825-182, Portugal.
authors.forename: Hugo M
authors.initials: HM
authors.lastname: Santos
keywords: 2D-Gel Electrophoresis; Peritoneal dialysis effluent; Protein identification; Proteomics
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