title: Preparation, evaluation and application of diazinon imprinted polymers as the sorbent in molecularly imprinted solid-phase extraction and liquid chromatography analysis in cucumber and aqueous samples.
abstract: A series of diazinon imprinted polymers (MIPs) were prepared and evaluated in the binding study in comparison with a non-imprinted polymer (NIP). The optimised MIP was evaluated as a sorbent, for extraction and preconcentration of diazinon from aqueous media and cucumber tissue. The HPLC-UV method was calibrated, in the range of 0.025-10 mg/kg. The results indicated that the optimised MIP had an excellent affinity for diazinon. The molecularly imprinted solid-phase extraction (MISPE) procedure was optimised with a recovery of 77-98%, in aqueous solution, and a recovery of 82-110%, in cucumber. The intra-day variation and inter-day variation values were less than 8.26% and 9.7%, respectively. Our data showed that, the MIP enabled the extraction of trace amounts of diazinon successfully from aqueous solution and cucumber, demonstrating the potential of MISPE for rapid, sensitive and cost-effective sample analysis.
authors.affiliation: Student Research Committee (SRC), Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Davood
authors.initials: D
authors.lastname: Davoodi
authors.affiliation: Pharmaceutical Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Hassanzadeh-Khayyat
authors.affiliation: Pharmaceutical Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Mitra Asgharian
authors.initials: MA
authors.lastname: Rezaei
authors.affiliation: Pharmaceutical Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address: mohajeria@mums.ac.ir.
authors.forename: Seyed Ahmad
authors.initials: SA
authors.lastname: Mohajeri
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods*; D018553:Cucumis sativus / Q000737:chemistry*; D003976:Diazinon / Q000494:pharmacology*; D007306:Insecticides / Q000494:pharmacology*; D011108:Polymers / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000379:methods; D014867:Water / Q000737:chemistry*
chemical_list: D007306:Insecticides; D011108:Polymers; D014867:Water; D003976:Diazinon
keywords: Affinity; Cucumber; Diazinon; Functional monomer; Molecularly imprinted polymer