title: Prevalence and characterization of uropathogenic Escherichia coli harboring plasmid-mediated quinolone resistance in a Tunisian university hospital.
abstract: The prevalence of plasmid-mediated quinolone resistance (PMQR) determinants was investigated in a Tunisian collection of 300 uropathogenic Escherichia coli. PMQR genes were detected in 68 isolates (22.7%) as follows: aac(6')-Ib-cr (n=66), qnrB1 (n=3), qnrA6 (n=1), and qnrS1 (n=1). Three isolates carried the 2 determinants aac(6')-Ib-cr and qnrB1. aac(6')-Ib-cr was usually carried on IncF-type plasmids (n=47/60) and frequently associated with blaCTX-M-15 (n=60). Substitutions in gyrA and parC genes were detected in 57.5% of strains. A major cluster including 29 isolates was individualized, 28 of them belonged to the virulent ST131 clone. In our hospital, the high prevalence of PMQR in E. coli isolates was due to horizontal transfer but also to the spread of ST131 clone.
authors.affiliation: Université de Tunis El Manar, Faculté de Médecine de Tunis, LR99ES09 Laboratoire de Résistance aux antimicrobiens, 1007, Tunis, Tunisia. Electronic address: ferjsana@yahoo.fr.
authors.forename: Ferjani
authors.initials: F
authors.lastname: Sana
authors.affiliation: Université de Tunis El Manar, Faculté de Médecine de Tunis, LR99ES09 Laboratoire de Résistance aux antimicrobiens, 1007, Tunis, Tunisia; CHU Charles Nicolle, Service de Microbiologie, 1006, Tunis, Tunisia.
authors.forename: Saidani
authors.initials: S
authors.lastname: Mabrouka
authors.affiliation: Université Bordeaux, Microbiologie Fondamentale et pathogénicité, UMR5234, Bordeaux, France.
authors.forename: Quentin
authors.initials: Q
authors.lastname: Claudine
authors.affiliation: CHU Charles Nicolle, Service de Microbiologie, 1006, Tunis, Tunisia.
authors.forename: Slim Amine
authors.initials: SA
authors.lastname: Faouzi
authors.affiliation: Université de Tunis El Manar, Faculté de Médecine de Tunis, LR99ES09 Laboratoire de Résistance aux antimicrobiens, 1007, Tunis, Tunisia; CHU Charles Nicolle, Service de Microbiologie, 1006, Tunis, Tunisia.
authors.forename: Boutiba Ben Boubaker
authors.initials: BB
authors.lastname: Ilhem
authors.affiliation: Université Bordeaux, Microbiologie Fondamentale et pathogénicité, UMR5234, Bordeaux, France.
authors.forename: Dubois
authors.initials: D
authors.lastname: Véronique
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002648:Child; D002675:Child, Preschool; D016000:Cluster Analysis; D024881:Drug Resistance, Bacterial; D004927:Escherichia coli Infections / Q000453:epidemiology* / Q000382:microbiology; D005260:Female; D005798:Genes, Bacterial; D006785:Hospitals, University; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D017720:Molecular Epidemiology; D058889:Molecular Typing; D010957:Plasmids / Q000032:analysis; D015995:Prevalence; D015363:Quinolones / Q000494:pharmacology*; D014416:Tunisia / Q000453:epidemiology; D056689:Uropathogenic Escherichia coli / Q000145:classification / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D037521:Virulence Factors / Q000235:genetics; D055815:Young Adult
chemical_list: D000900:Anti-Bacterial Agents; D015363:Quinolones; D037521:Virulence Factors
keywords: Escherichia coli; Phylogenetic groups; Plasmid-mediated quinolone resistance; ST131 clone; Uropathogenic strains; Virulence factors