title: The pattern of cytokine production in vitro induced by ancient and modern Beijing Mycobacterium tuberculosis strains.
abstract: It is unclear to what extent the host-responses elicited by Beijing versus non-Beijing strains of Mycobacterium tuberculosis (MTB) contribute to the predominance of modern Beijing strains in Taiwan and some other Asian countries. The purpose of this study was to compare the expression profiles of virulence-related genes in human monocyte-derived macrophages infected in vitro with Beijing (ancient and modern strains) and non-Beijing strains (EAI strains) of MTB that are epidemic in Taiwan. We found that modern Beijing strains induced lower levels of pro-inflammatory cytokines, whereas EAI strains induced higher levels. Notably, the most prevalent modern Beijing sub-lineage, possessing intact RD150 and RD142 chromosomal regions, induced very low levels of pro-inflammatory cytokines, especially interleukin-1β. Moreover, in an intracellular growth assay, the survival of the same modern Beijing strain in human monocyte-derived macrophages was significantly higher than that of an ancient Beijing strain and an EAI strain. Taken together, these results may explain why modern Beijing strains of MTB predominate in Taiwan.
authors.affiliation: National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Zhunan, Miaoli, Taiwan.
authors.forename: Yih-Yuan
authors.initials: YY
authors.lastname: Chen
authors.affiliation: National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Zhunan, Miaoli, Taiwan.
authors.forename: Jia-Ru
authors.initials: JR
authors.lastname: Chang
authors.affiliation: National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Zhunan, Miaoli, Taiwan.
authors.forename: Wei-Feng
authors.initials: WF
authors.lastname: Huang
authors.affiliation: National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Zhunan, Miaoli, Taiwan.
authors.forename: Shu-Ching
authors.initials: SC
authors.lastname: Hsu
authors.affiliation: National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Zhunan, Miaoli, Taiwan.
authors.forename: Shu-Chen
authors.initials: SC
authors.lastname: Kuo
authors.affiliation: Division of Clinical Pathology, Department of Pathology, Tri-Service General Hospital and National Defense Medical Center, Taipei, Taiwan.
authors.forename: Jun-Ren
authors.initials: JR
authors.lastname: Sun
authors.affiliation: National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Zhunan, Miaoli, Taiwan.
authors.forename: Horng-Yunn
authors.initials: HY
authors.lastname: Dou
mesh_terms: D015373:Bacterial Typing Techniques; D018925:Chemokines / Q000096:biosynthesis; D016000:Cluster Analysis; D016207:Cytokines / Q000096:biosynthesis*; D062665:Epidemiological Monitoring; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism; D008264:Macrophages / Q000382:microbiology / Q000473:pathology; D008297:Male; D009000:Monocytes / Q000473:pathology; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000254:growth & development / Q000502:physiology*; D010802:Phylogeny; D013624:Taiwan / Q000453:epidemiology; D014376:Tuberculosis / Q000453:epidemiology / Q000382:microbiology
chemical_list: D018925:Chemokines; D016207:Cytokines; D018836:Inflammation Mediators
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