title: Mesenchymal stem cells improved the ultrastructural morphology of cerebral tissues after subarachnoid hemorrhage in rats.
abstract: Subarachnoid hemorrhage (SAH) causes widespread disruption in the cerebral architecture.The process of SAH is complicated and many people lose their lives or become disabled after injury. Mesenchymal stem cells (MSCs) are considered as good candidate for repair of cerebral damage. The aim was to assess the ultrastructural changes in the rat cerebral tissue after intravenous transplantation of MSCs. Female Wistar rats (8 per group) weighing 275~300 g were assigned to control (SAH+PBS) and experimental groups (SAH+MSCs).The samples from middle cerebral arterial wall and parietal cerebral tissue were prepared for transmission electron microscopy (TEM) according to standard protocol. Fine architectures of the vessel wall, including the contraction of the inner layer, smooth muscle layer,as well as neural cells were observed after SAH. Cerebral arterial wall and cortex, including neuronal and glial cells were injured post SAH. But, administration of MSCs improved the structural integrity of cerebral tissues. Changes were much more balanced with their relative improvement in some areas. The role of MSCs for repairing the injured cerebral tissues post experimental SAH was approved by electron microscopy.
authors.affiliation: Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
authors.forename: Mohammad Ali
authors.initials: MA
authors.lastname: Khalili
authors.affiliation: Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. ; Stem Cell Biology Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Sadeghian-Nodoushan
authors.affiliation: Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
authors.forename: Farzaneh
authors.initials: F
authors.lastname: Fesahat
authors.affiliation: Yazd ACECR Higher Education Institute, Yazd, Iran. ; Department of Biology, Faculty of Science, Sistan & Baluchestan University, Zahedan, Iran.
authors.forename: Seyed Mohsen
authors.initials: SM
authors.lastname: Mir-Esmaeili
authors.affiliation: Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
authors.forename: Morteza
authors.initials: M
authors.lastname: Anvari
authors.affiliation: Department of Pathology, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
authors.forename: Seyed Hossain
authors.initials: SH
authors.lastname: Hekmati-Moghadam
keywords: Electron microscopy; Mesenchymal stem cell; Transplantation; rat; subarachnoid hemorrhage
references.citation: J Neurosurg. 1983 Jan;58(1):18-26
references.pmid: 6847905
references.citation: Acta Neuropathol. 1985;67(1-2):13-24
references.pmid: 4024866
references.citation: J Neurosurg. 1990 Apr;72(4):626-33
references.pmid: 2319321
references.citation: Stroke. 2010 Aug;41(8):e519-36
references.pmid: 20595669
references.citation: Stroke. 1993 Dec;24(12 Suppl):I84-90
references.pmid: 8249026
references.citation: Lancet Neurol. 2009 Jul;8(7):635-42
references.pmid: 19501022
references.citation: Front Cell Neurosci. 2012 Aug 02;6:30
references.pmid: 22876219
references.citation: Br J Anaesth. 2012 Sep;109(3):315-29
references.pmid: 22879655
references.citation: Stem Cells Cloning. 2011 Nov 17;4:61-72
references.pmid: 24198531
references.citation: Mol Ther. 2009 Jul;17(7):1282-91
references.pmid: 19436269
references.citation: J Zhejiang Univ Sci B. 2010 Jul;11(7):516-23
references.pmid: 20593517
references.citation: J Neurol Neurosurg Psychiatry. 1985 Mar;48(3):256-62
references.pmid: 3981195
references.citation: Stroke Res Treat. 2013;2013:425281
references.pmid: 23589781
references.citation: J Cereb Blood Flow Metab. 2014 Jan;34(1):108-17
references.pmid: 24064494
references.citation: J Neurosurg. 1978 Aug;49(2):239-48
references.pmid: 27590
references.citation: Neurosurgery. 1989 Nov;25(5):736-45; discussion 745-6
references.pmid: 2586727
references.citation: Pathophysiology. 2005 Jul;12(1):47-62
references.pmid: 15927824
references.citation: Neurosci Lett. 2002 Jun 28;326(2):137-41
references.pmid: 12057847
references.citation: J Neurosurg. 1983 Jan;58(1):11-7
references.pmid: 6847896
references.citation: Neurosci Lett. 2009 Jun 12;456(3):120-3
references.pmid: 19429146
references.citation: J Clin Neurosci. 2009 Jan;16(1):12-20
references.pmid: 19017556
references.citation: Biomaterials. 2009 Oct;30(28):4996-5003
references.pmid: 19539369
references.citation: Neuroscience. 2012 Nov 8;224:26-37
references.pmid: 22902542
references.citation: Cell Mol Neurobiol. 2009 Mar;29(2):235-41
references.pmid: 18821009
references.citation: Cytotherapy. 2007;9(2):170-83
references.pmid: 17453969
references.citation: J Stroke Cerebrovasc Dis. 2012 Aug;21(6):445-51
references.pmid: 21282068
references.citation: Vasc Health Risk Manag. 2012;8:569-80
references.pmid: 23091389
references.citation: Surg Neurol. 1990 Mar;33(3):195-201
references.pmid: 2315830
references.citation: Lancet Neurol. 2002 Jun;1(2):92-100
references.pmid: 12849513
references.citation: J Neurosurg. 1978 Aug;49(2):229-38
references.pmid: 671076
references.citation: Neurosci Lett. 2011 Jan 7;487(2):163-8
references.pmid: 20951186
references.citation: Cell Biol Int. 2011 Oct;35(10):1055-62
references.pmid: 21592091
references.citation: Lancet Neurol. 2011 Jul;10(7):618-25
references.pmid: 21640651