title: Selenium-enriched exopolysaccharides improve skeletal muscle glucose uptake of diabetic KKAy mice via AMPK pathway.
abstract: Selenium-enriched exopolysaccharides (EPS) produced by Enterobacter cloacae Z0206 have been proven to possess effect on reducing blood glucose level in diabetic mice. To investigate the specific mechanism, we studied the effects of oral supply with EPS on skeletal muscle glucose transportation and consumption in high-fat-diet-induced diabetic KKAy mice. We found that EPS supplementation increased expressions of glucose transporter 4 (Glut4), hexokinase 2 (hk2), phosphorylation of AMP-activated kinase subunit α2 (pAMPKα2), and peroxisome proliferator-activated receptor γ coactivator 1α (PGC-1α), and increased expression of characteristic protein of oxidative fibers such as troponin I and cytochrome c (Cytc). Furthermore, we found that EPS increased glucose uptake and expressions of pAMPKα2 and PGC-1α in palmitic acid (PA)-induced C2C12 cells. However, while EPS inhibited AMPKα2 with interference RNA (iRNA), effects of EPS on the improvement of glucose uptake diminished. These results indicated that EPS may improve skeletal muscle glucose uptake of diabetic KKAy mice through AMPKα2-PGC-1α pathway.
authors.affiliation: Key Laboratory of Animal Nutrition and Feed Science, Ministry of Agriculture, Zhejiang Provincial Laboratory of Feed and Animal Nutrition, Institute of Feed Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou, 310058, People Republic of China.
authors.forename: Xihong
authors.initials: X
authors.lastname: Zhou
authors.forename: Jingqing
authors.initials: J
authors.lastname: Chen
authors.forename: Fengqin
authors.initials: F
authors.lastname: Wang
authors.forename: Hangxian
authors.initials: H
authors.lastname: Yang
authors.forename: Ren
authors.initials: R
authors.lastname: Yang
authors.forename: Xinxia
authors.initials: X
authors.lastname: Wang
authors.forename: Yizhen
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000263:Adenylate Kinase / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D003470:Culture Media; D005947:Glucose / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000378:metabolism; D011134:Polysaccharides / Q000008:administration & dosage / Q000494:pharmacology*; D060888:Real-Time Polymerase Chain Reaction; D012643:Selenium / Q000008:administration & dosage*
chemical_list: D003470:Culture Media; D011134:Polysaccharides; D000263:Adenylate Kinase; D012643:Selenium; D005947:Glucose
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