title: Impairment of uterine smooth muscle contractions and prostaglandin secretion from cattle myometrium and corpus luteum in vitro is influenced by DDT, DDE and HCH.
abstract: The aim of this study was to investigate the effect of dichlorodiphenyltrichloroethane(DDT), dichlorodiphenyldichloroethylene (DDE) and γ-hexachlorocyclohexane (HCH) (10 ng/ml) on myometrial motility and the secretory function of the myometrium and corpus luteum (CL) collected from cows on days 8-12 of the estrous cycle. All of the xenobiotics increased (P<0.05) myometrial contractility. Moreover, the xenobiotics stimulated the secretion of the following prostaglandins (PGs) from myometrial strips: PGF2α, PGE2 and PGI2. DDT and DDE also increased (P<0.05) the release of PGF2α from CL strips, and HCH had the same effect (P<0.05) on the secretion of PGE2 and PGI2. The studied xenobiotics did not affect (P>0.05) PG synthesis, but DDT and DDE increased the mRNA expression levels of leukemia inhibitor factor (LIF), which can stimulate PG production. In summary, the xenobiotics affected PG secretion from cow myometrium and CL, which may contribute to the mechanism of uterine contraction disturbance.
authors.affiliation: Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima Street 10, 10- 748 Olsztyn, Poland.
authors.forename: Michal H
authors.initials: MH
authors.lastname: Wrobel
authors.affiliation: Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima Street 10, 10- 748 Olsztyn, Poland.
authors.forename: Pawel
authors.initials: P
authors.lastname: Bedziechowski
authors.affiliation: Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima Street 10, 10- 748 Olsztyn, Poland.
authors.forename: Jaroslaw
authors.initials: J
authors.lastname: Mlynarczuk
authors.affiliation: Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima Street 10, 10- 748 Olsztyn, Poland. Electronic address: j.kotwica@pan.olsztyn.pl.
authors.forename: Jan
authors.initials: J
authors.lastname: Kotwica
mesh_terms: D000818:Animals; D002417:Cattle; D003338:Corpus Luteum / Q000187:drug effects* / Q000378:metabolism; D030762:Estrous Cycle; D005260:Female; D006843:Hydrocarbons, Chlorinated / Q000633:toxicity*; D009215:Myometrium / Q000187:drug effects* / Q000378:metabolism; D011453:Prostaglandins / Q000096:biosynthesis / Q000378:metabolism*; D014590:Uterine Contraction / Q000187:drug effects*
chemical_list: D006843:Hydrocarbons, Chlorinated; D011453:Prostaglandins
keywords: Cow; LIF; Pesticides; Prostaglandins; Reproductive tract