title: The constitutive expression of a two transgene construct enhances the abiotic stress tolerance of chrysanthemum.
abstract: Various abiotic stresses downgrade the quality and productivity of chrysanthemum. A construct carrying both CcSOS1 (from Chrysanthemum crassum) and CdICE1 (from Chrysanthemum dichrum) was constitutively expressed in the chrysanthemum variety 'Jinba'. The transgenic plants were superior to the wild type (WT) ones with respect to their sensitivity to low temperature, drought and salinity, as measured by visible damage and plant survival. Salinity stressed transgenic plants accumulated more proline, and their level of superoxide dismutase and peroxidase activity was higher than in WT plants. At the physiological level, they suffered less loss of viable leaf area, maintained a lower leaf electrolyte conductivity and retained more chlorophyll (a+b). The ratio between the K(+) and Na(+) content was higher in the root, stem and median leaves of salinity stressed transgenic plants than in those of WT plants.
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Aiping
authors.initials: A
authors.lastname: Song
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Juan
authors.initials: J
authors.lastname: An
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Guan
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Jiafu
authors.initials: J
authors.lastname: Jiang
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Fadi
authors.initials: F
authors.lastname: Chen
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Wanghuai
authors.initials: W
authors.lastname: Lou
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Weimin
authors.initials: W
authors.lastname: Fang
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Zhaolei
authors.initials: Z
authors.lastname: Liu
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: chensm@njau.edu.cn.
authors.forename: Sumei
authors.initials: S
authors.lastname: Chen
mesh_terms: D002734:Chlorophyll / Q000378:metabolism; D031202:Chrysanthemum / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism*; D011392:Proline / Q000378:metabolism; D054712:Salinity
chemical_list: D010940:Plant Proteins; D002734:Chlorophyll; D011392:Proline
keywords: CcSOS1-CdICE1; Drought tolerance; Low temperature tolerance; Salinity tolerance