title: Immunohistochemical assessment of NY-ESO-1 expression in esophageal adenocarcinoma resection specimens.
abstract: OBJECTIVE
  To assess NY-ESO-1 expression in a cohort of esophageal adenocarcinomas.
  
  
  METHODS
  A retrospective search of our tissue archive for esophageal resection specimens containing esophageal adenocarcinoma was performed, for cases which had previously been reported for diagnostic purposes, using the systematised nomenclature of human and veterinary medicine coding system. Original haematoxylin and eosin stained sections were reviewed, using light microscopy, to confirm classification and tumour differentiation. A total of 27 adenocarcinoma resection specimens were then assessed using immunohistochemistry for NY-ESO-1 expression: 4 well differentiated, 14 moderately differentiated, 4 moderate-poorly differentiated, and 5 poorly differentiated.
  
  
  RESULTS
  Four out of a total of 27 cases of esophageal adenocarcinoma examined (15%) displayed diffuse cytoplasmic and nuclear expression for NY-ESO-1. They displayed a heterogeneous and mosaic-type pattern of diffuse staining. Diffuse cytoplasmic staining was not identified in any of these structures: stroma, normal squamous epithelium, normal submucosal gland and duct, Barrett's esophagus (goblet cell), Barrett's esophagus (non-goblet cell) and high grade glandular dysplasia. All adenocarcinomas showed an unexpected dot-type pattern of staining at nuclear, paranuclear and cytoplasmic locations. Similar dot-type staining, with varying frequency and size of dots, was observed on examination of Barrett's metaplasia, esophageal submucosal gland acini and the large bowel negative control, predominantly at the crypt base. Furthermore, a prominent pattern of apical (luminal) cytoplasmic dot-type staining was observed in some cases of Barrett's metaplasia and also adenocarcinoma. A further morphological finding of interest was noted on examination of haematoxylin and eosin stained sections, as aggregates of lymphocytes were consistently noted to surround submucosal glands.
  
  
  CONCLUSIONS
  We have demonstrated for the first time NY-ESO-1 expression by esophageal adenocarcinomas, Barrett's metaplasia and normal tissues other than germ cells.
authors.affiliation: Stephen J Hayes, Peter Clark, Department of Histopathology, Salford Royal NHS Foundation Trust, Salford M6 8HD, United Kingdom.
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Hayes
authors.affiliation: Stephen J Hayes, Peter Clark, Department of Histopathology, Salford Royal NHS Foundation Trust, Salford M6 8HD, United Kingdom.
authors.forename: Keng Ngee
authors.initials: KN
authors.lastname: Hng
authors.affiliation: Stephen J Hayes, Peter Clark, Department of Histopathology, Salford Royal NHS Foundation Trust, Salford M6 8HD, United Kingdom.
authors.forename: Peter
authors.initials: P
authors.lastname: Clark
authors.affiliation: Stephen J Hayes, Peter Clark, Department of Histopathology, Salford Royal NHS Foundation Trust, Salford M6 8HD, United Kingdom.
authors.forename: Fiona
authors.initials: F
authors.lastname: Thistlethwaite
authors.affiliation: Stephen J Hayes, Peter Clark, Department of Histopathology, Salford Royal NHS Foundation Trust, Salford M6 8HD, United Kingdom.
authors.forename: Robert E
authors.initials: RE
authors.lastname: Hawkins
authors.affiliation: Stephen J Hayes, Peter Clark, Department of Histopathology, Salford Royal NHS Foundation Trust, Salford M6 8HD, United Kingdom.
authors.forename: Yeng
authors.initials: Y
authors.lastname: Ang
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism* / Q000601:surgery; D000951:Antigens, Neoplasm / Q000235:genetics / Q000378:metabolism*; D001471:Barrett Esophagus / Q000378:metabolism / Q000601:surgery; D014408:Biomarkers, Tumor / Q000378:metabolism; D002454:Cell Differentiation; D004938:Esophageal Neoplasms / Q000378:metabolism* / Q000601:surgery; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D012189:Retrospective Studies; D013234:Stem Cells / Q000378:metabolism
chemical_list: D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; C104948:CTAG1B protein, human; D008565:Membrane Proteins
keywords: Esophageal adenocarcinoma; Immunohistochemistry; NY-ESO-1; Stem cells; Vesicle trafficking
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