title: [REGgamma promotes malignant behaviors of lung cancer cells].
abstract: OBJECTIVE
  To determine the expression of proteasome aotivator gamma (REGgamma) in human lung cancer tissues and cell lines and its association with malignant biological behaviors.
  
  
  METHODS
  Immunohistochemistry (IHC) was used to detect the expression of REGgamma in lung cancer and normal lung tissues. The expressions of REGgamma in lung cancer cells and normal epithelial cells were determined by Western blot. The H1975 lung cancer stable cell lines with different levels of REGgamma expression were constructed and their proliferations were evaluated by MTT assay. PI staining was used to assess the influence of REGgamma on cell growth cycle. The effect of REGgamma on the migration of lung cancer cells were observed with the cell scratch experiment.
  
  
  RESULTS
  Lung cancer tissues had significantly higher levels of REGgamma expression than normal tissues. Similarly, lung cancer cell lines showed higher levels of REGgamma expression than the normal epithelial cell line. The overexpression of REGgamma enhanced cancer cell proliferations (P < 0.05), promoted more cells into the S+G2/M phase (P < 0.05) and promoted the migration of cancer cells (P < 0.05). All of these effects were reversed after suppression of REGgamma.
  
  
  CONCLUSIONS
  REGgamma facilitates malignant biological behaviors of lung cancers.
authors.forename: Qing
authors.initials: Q
authors.lastname: Qin
authors.forename: Fu-Chun
authors.initials: FC
authors.lastname: Guo
authors.forename: Shun-Tao
authors.initials: ST
authors.lastname: Luo
authors.forename: Xiao-Yu
authors.initials: XY
authors.lastname: Li
authors.forename: Xiao-Ke
authors.initials: XK
authors.lastname: Liu
authors.forename: Yong-Sheng
authors.initials: YS
authors.lastname: Wang
mesh_terms: D001324:Autoantigens / Q000378:metabolism*; D015153:Blotting, Western; D002453:Cell Cycle; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology*; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism*
chemical_list: D001324:Autoantigens; C099558:Ki antigen; D046988:Proteasome Endopeptidase Complex