title: Expression of bone morphogenetic protein-6 in dental follicle stem cells and its effect on osteogenic differentiation.
abstract: The dental follicle (DF) plays an essential role in tooth eruption via regulation of bone resorption and bone formation. Bone morphogenetic protein-6 (BMP6) expression in the DF is coincident with bone growth in the tooth crypt. DF stem cells (DFSCs) have been shown to possess strong osteogenic capability. This study aims to determine the expression of BMP6 in DFSCs and to elucidate the role of BMP6 in the osteogenesis of DFSCs. DFSCs and their non-stem cell counterpart, DF cells (DFCs), were obtained from the DFs of rat pups. We showed that expression of BMP6 was significantly higher in the DFSCs than in the DFCs. DFSCs lost osteogenic capability during in vitro expansion, and DFSCs in late passages had reduced BMP6 expression as compared to early passages of DFSCs when they were subjected to osteogenic induction. Addition of exogenous human recombinant BMP6 (hrBMP6) to the osteogenic medium dramatically enhanced the osteogenesis of the late-passage DFSCs. Knockdown of BMP6 by short interfering RNA in the DFSCs in early passages resulted in a decrease in osteogenesis, which could be restored by addition of hrBMP6. We concluded that DFSCs need to express high levels of BMP6 to maintain their osteogenesis capability. Increased BMP6 expression seen in vivo in the DF may reflect the activation of DFSCs for osteogenic differentiation for bone growth during tooth eruption.
authors.affiliation: Department of Comparative Biomedical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, La., USA.
authors.forename: Shaomian
authors.initials: S
authors.lastname: Yao
authors.forename: Hongzhi
authors.initials: H
authors.lastname: He
authors.forename: Dina L
authors.initials: DL
authors.lastname: Gutierrez
authors.forename: Maryam Rezai
authors.initials: MR
authors.lastname: Rad
authors.forename: Dawen
authors.initials: D
authors.lastname: Liu
authors.forename: Chunhong
authors.initials: C
authors.lastname: Li
authors.forename: Michael
authors.initials: M
authors.lastname: Flanagan
authors.forename: Gary E
authors.initials: GE
authors.lastname: Wise
mesh_terms: D000818:Animals; D055418:Bone Morphogenetic Protein 6 / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects* / Q000235:genetics; D002478:Cells, Cultured; D003795:Dental Sac / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D010012:Osteogenesis / Q000187:drug effects* / Q000235:genetics; D034622:RNA Interference; D034741:RNA, Small Interfering; D051381:Rats; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*
chemical_list: C525816:BMP6 protein, human; D055418:Bone Morphogenetic Protein 6; D034741:RNA, Small Interfering
references.citation: Connect Tissue Res. 2010;51(1):59-66
references.pmid: 20067418
references.citation: J Oral Pathol. 1987 Apr;16(4):164-9
references.pmid: 3114454
references.citation: Biochem Biophys Res Commun. 2006 Oct 27;349(3):1050-6
references.pmid: 16965763
references.citation: J Biol Chem. 2006 Sep 1;281(35):25509-21
references.pmid: 16798745
references.citation: J Clin Invest. 1998 Jan 15;101(2):413-22
references.pmid: 9435314
references.citation: Biochem Biophys Res Commun. 2010 Oct 8;401(1):20-5
references.pmid: 20816936
references.citation: J Dent Res. 2008 Jul;87(7):676-81
references.pmid: 18573990
references.citation: J Biol Chem. 2008 Jul 25;283(30):20948-58
references.pmid: 18436533
references.citation: J Dent Res. 2008 May;87(5):414-34
references.pmid: 18434571
references.citation: Int Orthop. 2007 Dec;31(6):759-65
references.pmid: 17634942
references.citation: Eur J Oral Sci. 2006 Dec;114(6):512-6
references.pmid: 17184234
references.citation: Cytokine. 2003 Aug 21-Sep 7;23(4-5):133-7
references.pmid: 12967649
references.citation: J Dent Res. 2008 Aug;87(8):767-71
references.pmid: 18650550
references.citation: Stem Cells Int. 2012;2012:123030
references.pmid: 22645619
references.citation: J Oral Sci. 2010 Dec;52(4):541-52
references.pmid: 21206155
references.citation: J Cell Biochem. 2006 Jun 1;98(3):538-54
references.pmid: 16317727
references.citation: Endocrinology. 2001 Jun;142(6):2275-83
references.pmid: 11356673
references.citation: Lab Invest. 1990 Aug;63(2):144-61
references.pmid: 2116562
references.citation: J Cell Sci. 1999 Oct;112 ( Pt 20):3519-27
references.pmid: 10504300
references.citation: Clin Anat. 2007 Aug;20(6):632-9
references.pmid: 17415742
references.citation: Stem Cells. 2005 Oct;23(9):1357-66
references.pmid: 16081661
references.citation: Tissue Eng Part A. 2009 Feb;15(2):319-30
references.pmid: 19115826
references.citation: Eur J Oral Sci. 2011 Oct;119(5):333-8
references.pmid: 21896048
references.citation: Cell Tissue Res. 1992 Mar;267(3):483-92
references.pmid: 1571962