title: Use of advanced recombinant lines to study the impact and potential of mutations affecting starch synthesis in barley.
abstract: The effects on barley starch and grain properties of four starch synthesis mutations were studied during the introgression of the mutations from diverse backgrounds into an elite variety. Thelys5f(ADPglucose transporter),wax(granule-bound starch synthase),isa1(debranching enzyme isoamylase 1) andsex6(starch synthase IIa) mutations were introgressed into NFC Tipple to give mutant and wild-type BC2F4families with different genomic contributions of the donor parent. Comparison of starch and grain properties between the donor parents, the BC2F4families and NFC Tipple allowed the effects of the mutations to be distinguished from genetic background effects. Thewaxandsex6mutations had marked effects on starch properties regardless of genetic background. Thesex6mutation conditioned low grain weight and starch content, but thewaxmutation did not. Thelys5mutation conditioned low grain weight and starch content, but exceptionally high β-glucan contents. Theisa1 mutation promotes synthesis of soluble α-glucan (phytoglycogen). Its introgression into NFC Tipple increased grain weight and total α-glucan content relative to the donor parent, but reduced the ratio of phytoglycogen to starch. This study shows that introgression of mutations into a common, commercial background provides new insights that could not be gained from the donor parent.
authors.affiliation: Department of Metabolic Biology, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, United Kingdom.
authors.forename: Thomas P
authors.initials: TP
authors.lastname: Howard
authors.affiliation: Department of Metabolic Biology, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, United Kingdom.
authors.forename: Brendan
authors.initials: B
authors.lastname: Fahy
authors.affiliation: National Institute of Agricultural Botany, Huntingdon Road, Cambridge CB3 OLE, United Kingdom.
authors.forename: Fiona
authors.initials: F
authors.lastname: Leigh
authors.affiliation: National Institute of Agricultural Botany, Huntingdon Road, Cambridge CB3 OLE, United Kingdom.
authors.forename: Phil
authors.initials: P
authors.lastname: Howell
authors.affiliation: National Institute of Agricultural Botany, Huntingdon Road, Cambridge CB3 OLE, United Kingdom.
authors.forename: Wayne
authors.initials: W
authors.lastname: Powell
authors.affiliation: National Institute of Agricultural Botany, Huntingdon Road, Cambridge CB3 OLE, United Kingdom.
authors.forename: Andy
authors.initials: A
authors.lastname: Greenland
authors.affiliation: Department of Metabolic Biology, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, United Kingdom.
authors.forename: Kay
authors.initials: K
authors.lastname: Trafford
authors.affiliation: Department of Metabolic Biology, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, United Kingdom.
authors.forename: Alison M
authors.initials: AM
authors.lastname: Smith
keywords: Barley mutant; Barley starch; Starch properties
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