title: Homogeneity of VacJ outer membrane lipoproteins among Pasteurella multocida strains and heterogeneity among members of Pasteurellaceae.
abstract: Outer membrane lipoproteins are widely distributed in Gram-negative bacteria which are involved in diverse mechanisms of physiology/pathogenesis. Various pathogenic bacterial strains belonging to the family-Pasteurellaceae have several surface exposed virulence factors including VacJ/VacJ-like lipoproteins. In the present study, vacJ gene encoding for VacJ outer membrane lipoprotein of different Pasteurella multocida strains (n = 10) were amplified, sequenced and compared with available VacJ/VacJ-like sequences (n = 45) of Pasteurellaceae members. Comparative multiple sequence analysis at amino acid level indicated absolute homogeneity of VacJ lipoprotein among different P. multocida strains. However, heterogeneity (18.0-89.9%) of VacJ lipoprotein was noticed among members of Pasteurellaceae. A predicted lipobox motif (L-3-[A/S/T/V]-2-[G/A]-1-C) was found to be conserved between 12-32aa residues at N-terminus among all VacJ sequences. Bioinformatic analysis indicated that VacJ is a chromosomal gene product exposed on the bacterial surface, possibly essential for either physiological or pathogenicity process of Pasteurellae and distributed widely among P. multocida serogroups. The study indicated potential possibilities of using absolutely conserved VacJ lipoprotein either as 'signature gene/protein' in developing diagnostic assay or as a recombinant subunit vaccine for P. multocida infections in livestock.
authors.affiliation: Clinical Bacteriology Laboratory, Indian Veterinary Research Institute (IVRI), Mukteswar-263138, Nainital (District), Uttarakhand (UK), India. Electronic address: sbshivachandra@gmail.com.
authors.forename: Sathish Bhadravati
authors.initials: SB
authors.lastname: Shivachandra
authors.affiliation: Clinical Bacteriology Laboratory, Indian Veterinary Research Institute (IVRI), Mukteswar-263138, Nainital (District), Uttarakhand (UK), India.
authors.forename: Abhinendra
authors.initials: A
authors.lastname: Kumar
authors.affiliation: Clinical Bacteriology Laboratory, Indian Veterinary Research Institute (IVRI), Mukteswar-263138, Nainital (District), Uttarakhand (UK), India.
authors.forename: Nihar Nalini
authors.initials: NN
authors.lastname: Mohanty
authors.affiliation: National Institute of Veterinary Epidemiology and Disease Informatics (NIVEDI), Bengaluru-560024, Karnataka, India.
authors.forename: Revanaiah
authors.initials: R
authors.lastname: Yogisharadhya
authors.affiliation: Clinical Bacteriology Laboratory, Indian Veterinary Research Institute (IVRI), Mukteswar-263138, Nainital (District), Uttarakhand (UK), India.
authors.forename: Nirmal
authors.initials: N
authors.lastname: Chacko
authors.affiliation: Division of Bacteriology and Mycology, Indian Veterinary Research Institute (IVRI), Izatnagar-243122, Uttar Pradesh (UP), India.
authors.forename: K N
authors.initials: KN
authors.lastname: Viswas
authors.affiliation: Division of Virology, Indian Veterinary Research Institute (IVRI), Mukteswar-263138, Nainital (District), Uttarakhand (UK), India.
authors.forename: Muthannan Andavar
authors.initials: MA
authors.lastname: Ramakrishnan
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D019295:Computational Biology; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D014644:Genetic Variation / Q000235:genetics*; D008969:Molecular Sequence Data; D016979:Pasteurella multocida / Q000235:genetics*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D037521:Virulence Factors / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D037521:Virulence Factors
keywords: Heterogeneity; Homogeneity; Pasteurella multocida; Pasteurellaceae; Sequence analysis; VacJ outer membrane lipoprotein