title: Enhancement of the cytotoxicity and selectivity of doxorubicin to hepatoma cells by synergistic combination of galactose-decorated γ-poly(glutamic acid) nanoparticles and low-intensity ultrasound.
abstract: Specific drug delivery to solid tumors remains one of the challenges in cancer therapy. The aim of this study was to combine three drug-targeting strategies, polymer-drug conjugate, ligand presentation and ultrasound treatment, to enhance the efficacy and selectivity of doxorubicin (DXR) to hepatoma cells. The conjugation of DXR to γ-poly(glutamic acids) (γ-PGA) decreased the cytotoxicity of DXR, while the conjugation of galactosamine (Gal) to the γ-PGA-DXR conjugate restored the cytotoxic efficacy of DXR on hepatoma cells due to increased uptake of DXR. Furthermore, low-intensity ultrasound treatment increased the cell-killing ability of γ-PGA-DXR conjugates by 20%. The in vitro results showed the potential of the γ-PGA-DXR-Gal conjugate for future clinical applications.
authors.affiliation: Department of Chemical Engineering, National Taiwan University , Number 1, Section 4, Roosevelt Road, Taipei 106, Taiwan.
authors.forename: Wei-Bor
authors.initials: WB
authors.lastname: Tsai
authors.forename: Hsin-Yu
authors.initials: HY
authors.lastname: Lai
authors.forename: Jyun-Lin
authors.initials: JL
authors.lastname: Lee
authors.forename: Chia-Wen
authors.initials: CW
authors.lastname: Lo
authors.forename: Wen-Shiang
authors.initials: WS
authors.lastname: Chen
mesh_terms: D006528:Carcinoma, Hepatocellular; D002470:Cell Survival / Q000187:drug effects; D004317:Doxorubicin / Q000737:chemistry* / Q000494:pharmacology*; D016503:Drug Delivery Systems; D005690:Galactose / Q000737:chemistry*; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D014407:Tumor Cells, Cultured; D014465:Ultrasonics
chemical_list: D004317:Doxorubicin; D005690:Galactose