title: Differentiation, polarization, and migration of human induced pluripotent stem cell-derived neural progenitor cells co-cultured with a human glial cell line with radial glial-like characteristics.
abstract: Here we established a unique human glial cell line, GDC90, derived from a human glioma and demonstrated its utility as a glial scaffold for the polarization and differentiation of human induced pluripotent stem cell-derived neural progenitor cells (iPSC-NPCs). When co-cultured with GDC90 cells, iPSC-NPCs underwent rapid polarization and neurite extension along the radially spreading processes of the GDC90 cells, and showed migratory behavior. This method is potentially useful for detailed examination of neurites or for controlling neurites behavior for regenerative medicine.
authors.affiliation: Department of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan; Division of Regenerative Medicine, Institute for Clinical Research, Osaka National Hospital, National Hospital Organization, 2-1-14 Hoenzaka, Chuo-ku, Osaka 540-0006, Japan.
authors.forename: Yohei
authors.initials: Y
authors.lastname: Bamba
authors.affiliation: Division of Stem Cell Research, Institute for Clinical Research, Osaka National Hospital, National Hospital Organization, Osaka, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Shofuda
authors.affiliation: Division of Regenerative Medicine, Institute for Clinical Research, Osaka National Hospital, National Hospital Organization, 2-1-14 Hoenzaka, Chuo-ku, Osaka 540-0006, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Kanematsu
authors.affiliation: Department of Neurosurgery, Osaka National Hospital, National Hospital Organization, Osaka, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Nonaka
authors.affiliation: Department of Pediatric Neurosurgery, Takatsuki General Hospital, Takatsuki, Japan; Division of Molecular Medicine, Institute for Clinical Research, Osaka National Hospital, National Hospital Organization, Osaka, Japan.
authors.forename: Mami
authors.initials: M
authors.lastname: Yamasaki
authors.affiliation: Department of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan.
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Okano
authors.affiliation: Division of Regenerative Medicine, Institute for Clinical Research, Osaka National Hospital, National Hospital Organization, 2-1-14 Hoenzaka, Chuo-ku, Osaka 540-0006, Japan; Department of Neurosurgery, Osaka National Hospital, National Hospital Organization, Osaka, Japan. Electronic address: kanemura@onh.go.jp.
authors.forename: Yonehiro
authors.initials: Y
authors.lastname: Kanemura
mesh_terms: D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology; D016764:Cell Polarity / Q000502:physiology; D048430:Cell Shape; D018920:Coculture Techniques / Q000379:methods; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000502:physiology*; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008567:Membranes, Artificial; D009416:Nerve Regeneration; D058953:Neural Stem Cells / Q000166:cytology* / Q000502:physiology*; D016501:Neurites / Q000502:physiology; D009457:Neuroglia / Q000166:cytology* / Q000502:physiology*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D044968:Regenerative Medicine / Q000379:methods; D014162:Transfection
chemical_list: D008164:Luminescent Proteins; D008567:Membranes, Artificial; D011994:Recombinant Proteins
keywords: Glioma; Induced pluripotent stem cells; Neuronal migration; PiggyBac transposon; Radial glia; Regenerative medicine