title: Covalent deposition of zwitterionic polymer and citric acid by click chemistry-enabled layer-by-layer assembly for improving the blood compatibility of polysulfone membrane.
abstract: Development of blood compatible membranes is critical for biomedical applications. Zwitterionic polymers have been proved to be resistant to nonspecific protein adsorption and platelet adhesion. In this work, two kinds of zwitterionic copolymers bearing alkynyl and azide groups are synthesized by atom transfer radical polymerization (ATRP) and subsequent reactions, namely alkynyl-poly(sulfobetaine methacrylate) (alkynyl-PSBMA) and azide-poly(sulfobetaine methacrylate) (azide-PSBMA). The copolymers are directly used to modify azido-functionalized polysulfone (PSf-N3) membrane via click chemistry-enabled layer-by-layer (LBL) assembly. Alkynyl-citric acid is then clicked onto the membrane when the outermost layer was azide-PSBMA. The chemical compositions, surface morphologies, and hydrophilicity of the zwitterionic polymer and citric acid multilayer modified membranes are characterized. The composite multilayer is resistant to protein adsorption and platelet adhesion and also prolongs clotting times, indicating that the blood compatibility is improved. Moreover, after clicking the small molecule anticoagulant alkynyl-citric acid onto the outermost of the zwitterionic multilayer, the membrane shows further improved anticoagulant property. The deposition of zwitterionic polymer and citric acid via click chemistry-enabled LBL assembly can improve the blood compatibility of the PSf membrane.
authors.affiliation: College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University , Chengdu 610065, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Xiang
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
authors.forename: Wei-Feng
authors.initials: WF
authors.lastname: Zhao
authors.forename: Shu-Dong
authors.initials: SD
authors.lastname: Sun
authors.forename: Chang-Sheng
authors.initials: CS
authors.lastname: Zhao
mesh_terms: D001672:Biocompatible Materials / Q000009:adverse effects / Q000737:chemistry*; D019343:Citric Acid / Q000737:chemistry*; D057930:Click Chemistry / Q000379:methods*; D006801:Humans; D008567:Membranes, Artificial; D011108:Polymers / Q000737:chemistry*; D013450:Sulfones / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D008567:Membranes, Artificial; D011108:Polymers; D013450:Sulfones; C017662:polysulfone P 1700; D019343:Citric Acid