title: Fast and economic immobilization methods described for non-commercial Pseudomonas lipases.
abstract: BACKGROUND
  There is an increasing interest to seek new enzyme preparations for the development of new products derived from bioprocesses to obtain alternative bio-based materials. In this context, four non-commercial lipases from Pseudomonas species were prepared, immobilized on different low-cost supports, and examined for potential biotechnological applications.
  
  
  RESULTS
  To reduce costs of eventual scaling-up, the new lipases were obtained directly from crude cell extracts or from growth culture supernatants, and immobilized by simple adsorption on Accurel EP100, Accurel MP1000 and Celite®545. The enzymes evaluated were LipA and LipC from Pseudomonas sp. 42A2, a thermostable mutant of LipC, and LipI.3 from Pseudomonas CR611, which were produced in either homologous or heterologous hosts. Best immobilization results were obtained on Accurel EP100 for LipA and on Accurel MP1000 for LipC and its thermostable variant. Lip I.3, requiring a refolding step, was poorly immobilized on all supports tested (best results for Accurel MP1000). To test the behavior of immobilized lipases, they were assayed in triolein transesterification, where the best results were observed for lipases immobilized on Accurel MP1000.
  
  
  CONCLUSIONS
  The suggested protocol does not require protein purification and uses crude enzymes immobilized by a fast adsorption technique on low-cost supports, which makes the method suitable for an eventual scaling up aimed at biotechnological applications. Therefore, a fast, simple and economic method for lipase preparation and immobilization has been set up. The low price of the supports tested and the simplicity of the procedure, skipping the tedious and expensive purification steps, will contribute to cost reduction in biotechnological lipase-catalyzed processes.
authors.forename: Silvia
authors.initials: S
authors.lastname: Cesarini
authors.forename: Belén
authors.initials: B
authors.lastname: Infanzón
authors.forename: F I Javier
authors.initials: FI
authors.lastname: Pastor
authors.affiliation: Department of Microbiology, University of Barcelona, Av, Diagonal 643, 08028 Barcelona, Spain. pdiaz@ub.edu.
authors.forename: Pilar
authors.initials: P
authors.lastname: Diaz
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry; D001709:Biotechnology / Q000379:methods*; D004800:Enzymes, Immobilized / Q000737:chemistry*; D008049:Lipase / Q000737:chemistry*; D011549:Pseudomonas / Q000201:enzymology*
chemical_list: D001426:Bacterial Proteins; D004800:Enzymes, Immobilized; C079999:LipA protein, Bacteria; D008049:Lipase
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