title: Simple and easy DNA mapping method using peptide nucleic acid (PNA) tagging.
abstract: In this paper, we introduce a simple and easy DNA mapping method using peptide nucleic acid (PNA) on glass cover-slips based on optical detection. PNA and glass cover-slips were used as easy tagging and stretching method, separately. The PNA can be invaded lambda (A) DNA at the sequences we wished to tag without any additional materials. Alexa-488 fluorophore-conjugated PNA was designed to invade at four sites (sequence: AAGAAGAA) of lambda DNA with robust and exact binding. Fluorophore tagged lambda DNA was stretched in electrical layer coated glass cover-slips. The lambda DNA was stretched as 9.2 um in length. It is approximately 60% of theoretical length of lambda DNA and sufficient length to promise optical resolution. The sites of desired sequence tagged by PNA were well detected in stretched lambda DNA.
authors.forename: J
authors.initials: J
authors.lastname: Kim
authors.forename: J H
authors.initials: JH
authors.lastname: Park
authors.forename: S
authors.initials: S
authors.lastname: Lee
authors.forename: H J
authors.initials: HJ
authors.lastname: Shin
mesh_terms: D010582:Bacteriophage lambda / Q000378:metabolism; D001483:Base Sequence; D002874:Chromosome Mapping / Q000379:methods*; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D004279:DNA, Viral / Q000737:chemistry; D004563:Electrochemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D005898:Glass; D006834:Hydrazines / Q000737:chemistry; D008969:Molecular Sequence Data; D055095:Optics and Photonics; D020135:Peptide Nucleic Acids / Q000737:chemistry*; D017422:Sequence Analysis, DNA
chemical_list: C417561:Alexa 488 hydrazide; D004279:DNA, Viral; D005456:Fluorescent Dyes; D006834:Hydrazines; D020135:Peptide Nucleic Acids; D004247:DNA