title: Vaginal myeloid dendritic cells transmit founder HIV-1.
abstract: We report that primary human vaginal dendritic cells (DCs) display a myeloid phenotype and express CD4, CCR5, and CXCR4. Vaginal CD13(+) CD11c(+) DCs rapidly and efficiently bound transmitted/founder (T/F) CCR5-tropic (R5) viruses, transported them through explanted vaginal mucosa, and transmitted them in trans to vaginal and blood lymphocytes. Vaginal myeloid DCs may play a key role in capturing and disseminating T/F R5 HIV-1 in vivo and are candidate "gatekeeper" cells in HIV-1 transmission.
authors.affiliation: Department of Medicine (Gastroenterology), University of Alabama at Birmingham, Birmingham, Alabama, USA rshen@uab.edu pdsmith@uab.edu.
authors.forename: Ruizhong
authors.initials: R
authors.lastname: Shen
authors.affiliation: Department of Medicine (Hematology/Oncology), University of Alabama at Birmingham, Birmingham, Alabama, USA VA Medical Center Research Service, Birmingham, Alabama, USA.
authors.forename: John C
authors.initials: JC
authors.lastname: Kappes
authors.affiliation: Department of Medicine (Gastroenterology), University of Alabama at Birmingham, Birmingham, Alabama, USA.
authors.forename: Lesley E
authors.initials: LE
authors.lastname: Smythies
authors.affiliation: Department of Obstetrics and Gynecology, University of Alabama at Birmingham, Birmingham, Alabama, USA.
authors.forename: Holly E
authors.initials: HE
authors.lastname: Richter
authors.affiliation: Department of Pathology, University of Alabama at Birmingham, Birmingham, Alabama, USA.
authors.forename: Lea
authors.initials: L
authors.lastname: Novak
authors.affiliation: Department of Medicine (Gastroenterology), University of Alabama at Birmingham, Birmingham, Alabama, USA VA Medical Center Research Service, Birmingham, Alabama, USA rshen@uab.edu pdsmith@uab.edu.
authors.forename: Phillip D
authors.initials: PD
authors.lastname: Smith
mesh_terms: D002478:Cells, Cultured; D003713:Dendritic Cells / Q000378:metabolism / Q000821:virology*; D005260:Female; D015658:HIV Infections / Q000378:metabolism / Q000635:transmission*; D015497:HIV-1 / Q000472:pathogenicity*; D006801:Humans; D022423:Myeloid Cells / Q000378:metabolism / Q000821:virology*; D019713:Receptors, CCR5 / Q000378:metabolism; D013601:T-Lymphocytes / Q000378:metabolism / Q000821:virology*; D014621:Vagina / Q000378:metabolism / Q000821:virology*
chemical_list: D019713:Receptors, CCR5
references.citation: PLoS Pathog. 2009 Jul;5(7):e1000384
references.pmid: 19649323
references.citation: Immunity. 2007 Feb;26(2):257-70
references.pmid: 17306567
references.citation: Immunity. 2002 Jan;16(1):135-44
references.pmid: 11825572
references.citation: J Leukoc Biol. 2003 Nov;74(5):683-90
references.pmid: 12960236
references.citation: J Virol. 2009 Apr;83(7):3258-67
references.pmid: 19153236
references.citation: Trends Immunol. 2007 Nov;28(11):503-10
references.pmid: 17950666
references.citation: J Virol. 2002 Feb;76(4):1866-75
references.pmid: 11799181
references.citation: J Virol. 2000 Jul;74(13):6087-95
references.pmid: 10846092
references.citation: Virology. 2010 Dec 5;408(1):1-13
references.pmid: 20863545
references.citation: Nat Rev Immunol. 2008 Jun;8(6):435-46
references.pmid: 18500229
references.citation: Cell. 2000 Mar 3;100(5):587-97
references.pmid: 10721995
references.citation: Nat Immunol. 2002 Oct;3(10):975-83
references.pmid: 12352970
references.citation: Am J Reprod Immunol. 2014 Jun;71(6):608-17
references.pmid: 24689653
references.citation: Nat Rev Immunol. 2008 Dec;8(12):935-47
references.pmid: 19029989
references.citation: J Exp Med. 1996 Jan 1;183(1):215-25
references.pmid: 8551225
references.citation: Nat Rev Immunol. 2006 Nov;6(11):859-68
references.pmid: 17063186
references.citation: AIDS. 2011 Nov 13;25(17):2099-104
references.pmid: 21832933
references.citation: J Leukoc Biol. 2010 Apr;87(4):663-70
references.pmid: 20007245
references.citation: J Immunol. 2010 Apr 1;184(7):3648-55
references.pmid: 20208001
references.citation: Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):6626-33
references.pmid: 23542380
references.citation: Am J Reprod Immunol. 2011 Mar;65(3):261-7
references.pmid: 21118402
references.citation: J Virol. 2005 May;79(9):5762-73
references.pmid: 15827191
references.citation: Proc Natl Acad Sci U S A. 2008 May 27;105(21):7552-7
references.pmid: 18490657
references.citation: Proc Natl Acad Sci U S A. 2003 Jul 8;100(14):8401-6
references.pmid: 12815099
references.citation: J Exp Med. 1998 May 18;187(10):1623-31
references.pmid: 9584140