title: Effects of chitin and sepia ink hybrid hemostatic sponge on the blood parameters of mice.
abstract: Chitin and sepia ink hybrid hemostatic sponge (CTSH sponge), a new biomedical material, was extensively studied for its beneﬁcial biological properties of hemostasis and stimulation of healing. However, studies examining the safety of CTSH sponge in the blood system are lacking. This experiment aimed to examine whether CTSH sponge has negative effect on blood systems of mice, which were treated with a dosage of CTSH sponge (135 mg/kg) through a laparotomy. CTSH sponge was implanted into the abdominal subcutaneous and a laparotomy was used for blood sampling from abdominal aortic. Several kinds of blood parameters were detected at different time points, which were reflected by coagulation parameters including thrombin time (TT), prothrombin time (PT), activated partial thromboplatin time (APTT), fibrinogen (FIB) and platelet factor 4 (PF4); anticoagulation parameter including antithrombin III (AT-III); fibrinolytic parameters including plasminogen (PLG), fibrin degradation product (FDP) and D-dimer; hemorheology parameters including blood viscosity (BV) and plasma viscosity (PV). Results showed that CTSH sponge has no significant effect on the blood parameters of mice. The data suggested that CTSH sponge can be applied in the ﬁeld of biomedical materials and has potential possibility to be developed into clinical drugs of hemostatic agents.
authors.affiliation: Zhanjiang Normal University, Round Beibu Gulf Institute for the Protection and Utilization of Marine Animals in Medicine, Zhanjiang 524048, China. khzhangwei110@sina.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Zhanjiang Normal University, Round Beibu Gulf Institute for the Protection and Utilization of Marine Animals in Medicine, Zhanjiang 524048, China. sunyulin07002@126.com.
authors.forename: Yu-Lin
authors.initials: YL
authors.lastname: Sun
authors.affiliation: Zhanjiang Normal University, Round Beibu Gulf Institute for the Protection and Utilization of Marine Animals in Medicine, Zhanjiang 524048, China. dhchen11@21cn.com.
authors.forename: Dao-Hai
authors.initials: DH
authors.lastname: Chen
mesh_terms: D000818:Animals; D001012:Aorta, Abdominal / Q000378:metabolism; D001777:Blood Coagulation / Q000187:drug effects; D001809:Blood Viscosity / Q000187:drug effects; D002686:Chitin / Q000737:chemistry*; D005260:Female; D006490:Hemostatics / Q000737:chemistry*; D007813:Laparotomy / Q000379:methods; D008297:Male; D051379:Mice; D011517:Prothrombin Time; D049833:Sepia / Q000737:chemistry*; D015503:Surgical Sponges; D013918:Thrombin Time; D013997:Time Factors
chemical_list: D006490:Hemostatics; D002686:Chitin
references.citation: J Control Release. 2004 Apr 28;96(2):285-300
references.pmid: 15081219
references.citation: J Biomed Mater Res. 1996 Jun;31(2):158-66
references.pmid: 8731204
references.citation: Biomacromolecules. 2009 Jun 8;10(6):1603-11
references.pmid: 19391583
references.citation: Mar Drugs. 2009;7(1):9-18
references.pmid: 19370167
references.citation: Herz. 2003 Sep;28(6):530-8
references.pmid: 14569394
references.citation: J Food Sci Technol. 2014 Dec;51(12):3680-90
references.pmid: 25477634
references.citation: Neurol Med Chir (Tokyo). 2003 Feb;43(2):85-7
references.pmid: 12627886
references.citation: Yakugaku Zasshi. 2000 Nov;120(11):1075-89
references.pmid: 11190195
references.citation: Surgery. 1997 Mar;121(3):314-9
references.pmid: 9068673
references.citation: Pigment Cell Res. 2006 Dec;19(6):572-94
references.pmid: 17083485
references.citation: J Med Toxicol. 2013 Mar;9(1):49-53
references.pmid: 22733602
references.citation: Bioresour Technol. 2008 Mar;99(5):1359-67
references.pmid: 17383869
references.citation: J Oral Maxillofac Surg. 1999 Jan;57(1):49-52
references.pmid: 9915395
references.citation: Surg Endosc. 2011 Apr;25(4):1148-52
references.pmid: 20835722
references.citation: Clin Hemorheol Microcirc. 2003;29(2):129-37
references.pmid: 14610308
references.citation: Thromb Haemost. 2010 Feb;103(2):277-83
references.pmid: 20024502
references.citation: Biomaterials. 2003 Jun;24(13):2339-49
references.pmid: 12699672
references.citation: J Transl Med. 2012 Feb 10;10:28
references.pmid: 22325452
references.citation: Clin Oral Investig. 2013 May;17(4):1273-8
references.pmid: 22855267
references.citation: Atherosclerosis. 2010 Jun;210(2):671-3
references.pmid: 20079495