title: Comprehensive analysis of human cells motion under an irrotational AC electric field in an electro-microfluidic chip.
abstract: AC electrokinetics is a versatile tool for contact-less manipulation or characterization of cells and has been widely used for separation based on genotype translation to electrical phenotypes. Cells responses to an AC electric field result in a complex combination of electrokinetic phenomena, mainly dielectrophoresis and electrohydrodynamic forces. Human cells behaviors to AC electrokinetics remain unclear over a large frequency spectrum as illustrated by the self-rotation effect observed recently. We here report and analyze human cells behaviors in different conditions of medium conductivity, electric field frequency and magnitude. We also observe the self-rotation of human cells, in the absence of a rotational electric field. Based on an analytical competitive model of electrokinetic forces, we propose an explanation of the cell self-rotation. These experimental results, coupled with our model, lead to the exploitation of the cell behaviors to measure the intrinsic dielectric properties of JURKAT, HEK and PC3 human cell lines.
authors.affiliation: Univ. Grenoble Alpes, LTM, Grenoble, France; CNRS, LTM, Grenoble, France.
authors.forename: Clarisse
authors.initials: C
authors.lastname: Vaillier
authors.affiliation: Univ. Grenoble Alpes, LTM, Grenoble, France; CNRS, LTM, Grenoble, France; Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of Amercia.
authors.forename: Thibault
authors.initials: T
authors.lastname: Honegger
authors.affiliation: CEA, Institut de Recherches en Technologies et Sciences pour le Vivant, Grenoble, France.
authors.forename: Frédérique
authors.initials: F
authors.lastname: Kermarrec
authors.affiliation: CEA, Institut de Recherches en Technologies et Sciences pour le Vivant, Grenoble, France.
authors.forename: Xavier
authors.initials: X
authors.lastname: Gidrol
authors.affiliation: Univ. Grenoble Alpes, LTM, Grenoble, France; CNRS, LTM, Grenoble, France.
authors.forename: David
authors.initials: D
authors.lastname: Peyrade
mesh_terms: D002460:Cell Line; D002465:Cell Movement; D004560:Electricity; D006801:Humans; D046210:Microfluidic Analytical Techniques; D012399:Rotation
references.citation: PLoS One. 2013;8(1):e51577
references.pmid: 23320067
references.citation: Phys Rev E Stat Phys Plasmas Fluids Relat Interdiscip Topics. 2000 Apr;61(4 Pt B):4019-28
references.pmid: 11088193
references.citation: Electrophoresis. 2003 Nov;24(21):3703-17
references.pmid: 14613196
references.citation: Electrophoresis. 2011 Sep;32(18):2466-87
references.pmid: 21922493
references.citation: Lab Chip. 2013 Oct 21;13(20):4109-17
references.pmid: 23970334
references.citation: Lab Chip. 2013 Apr 21;13(8):1538-45
references.pmid: 23429670
references.citation: Biophys J. 1990 Feb;57(2):173-82
references.pmid: 2317544
references.citation: Biophys J. 1997 Aug;73(2):1118-29
references.pmid: 9251828
references.citation: Integr Biol (Camb). 2011 Jan;3(1):48-56
references.pmid: 20949196
references.citation: Chem Soc Rev. 2010 Mar;39(3):1203-17
references.pmid: 20179832
references.citation: Anal Chem. 2007 Sep 15;79(18):6975-87
references.pmid: 17702529
references.citation: Biomicrofluidics. 2012 Dec 12;6(4):44115
references.pmid: 24339848
references.citation: Lab Chip. 2013 Feb 21;13(4):589-98
references.pmid: 23314575
references.citation: Lab Chip. 2006 Jun;6(6):724-34
references.pmid: 16738722
references.citation: Anal Chem. 2009 Apr 1;81(7):2446-55
references.pmid: 19253950
references.citation: J Biomed Biotechnol. 2010;2010:182581
references.pmid: 20490279
references.citation: Langmuir. 2010 Mar 2;26(5):3441-52
references.pmid: 19957941
references.citation: Lab Chip. 2004 Aug;4(4):299-309
references.pmid: 15269795
references.citation: IEE Proc Nanobiotechnol. 2003 Nov;150(2):76-81
references.pmid: 16468935
references.citation: IEE Proc Nanobiotechnol. 2005 Dec;152(6):189-93
references.pmid: 16441179
references.citation: Biomicrofluidics. 2013 Oct 03;7(5):54112
references.pmid: 24404075
references.citation: Lab Chip. 2010 Mar 21;10(6):718-26
references.pmid: 20221559
references.citation: Lab Chip. 2010 Feb 21;10(4):438-45
references.pmid: 20126683
references.citation: Lab Chip. 2009 Jan 7;9(1):62-78
references.pmid: 19209337
references.citation: IEEE Eng Med Biol Mag. 2003 Nov-Dec;22(6):33-42
references.pmid: 15007989