title: Knockdown of zebrafish blood vessel epicardial substance results in incomplete retinal lamination.
abstract: Cell polarity during eye development determines the normal retinal lamination and differentiation of photoreceptor cells in the retina. In vertebrates, blood vessel epicardial substance (Bves) is known to play an important role in the formation and maintenance of the tight junctions essential for epithelial cell polarity. In the current study, we generated a transgenic zebrafish Bves (zbves) promoter-EGFP zebrafish line to investigate the expression pattern of Bves in the retina and to study the role of zbves in retinal lamination. Immunostaining with different specific antibodies from retinal cells and transmission electron microscopy were used to identify the morphological defects in normal and Bves knockdown zebrafish. In normal zebrafish, Bves is located at the apical junctions of embryonic retinal neuroepithelia during retinogenesis; later, it is strongly expressed around inner plexiform layer (IPL) and retinal pigment epithelium (RPE). In contrast, a loss of normal retinal lamination and cellular polarity was found with undifferentiated photoreceptor cells in Bves knockdown zebrafish. Herein, our results indicated that disruption of Bves will result in a loss of normal retinal lamination.
authors.affiliation: Department of Life Science, College of Life Science, National Taiwan University, Taipei 10617, Taiwan.
authors.forename: Yu-Ching
authors.identifier: 0000-0002-6989-6525
authors.initials: YC
authors.lastname: Wu
authors.affiliation: Department of Life Science, College of Life Science, National Taiwan University, Taipei 10617, Taiwan.
authors.forename: Ruei-Feng
authors.initials: RF
authors.lastname: Chen
authors.affiliation: Department of Ophthalmology, Graduate Program of Neuroscience, University of Cincinnati College of Medicine, Cincinnati, OH 45267, USA.
authors.forename: Chia-Yang
authors.initials: CY
authors.lastname: Liu
authors.affiliation: Department of Ophthalmology, National Taiwan University Hospital, Taipei 10002, Taiwan.
authors.forename: Fung Rong
authors.initials: FR
authors.lastname: Hu
authors.affiliation: Institute of Biological Chemistry, Academia Sinica, Taipei 11529, Taiwan.
authors.forename: Chang-Jen
authors.initials: CJ
authors.lastname: Huang
authors.affiliation: Department of Ophthalmology, National Taiwan University Hospital, Taipei 10002, Taiwan.
authors.forename: I-Jong
authors.initials: IJ
authors.lastname: Wang
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D016764:Cell Polarity; D017931:DNA Primers; D012160:Retina / Q000166:cytology*; D012171:Retinal Vessels / Q000378:metabolism*; D015027:Zebrafish
chemical_list: D017931:DNA Primers
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