title: Enhancing Corynebacterium glutamicum robustness by over-expressing a gene, mshA, for mycothiol glycosyltransferase.
abstract: Over-expression of the gene, mshA, coding for mycothiol glycosyl transferase improved the robustness of Corynebacterium glutamicum to various stresses. Intracellular mycothiol (MSH) content was increased by 114 % in WT(pXMJ19-mshA) compared to WT(pXMJ19). Survival rates increased by 44, 39, 90, 77, 131, 87, 52, 47, 57, 85 and 33 % as compared to WT(pXMJ19) under stress by H2O2 (40 mM), methylglyoxal (5.8 mM), erythromycin (0.08 mg ml(-1)), streptomycin (0.005 mg ml(-1)), Cd(2+) (0.01 mM), Mn(2+) (2 mM), formic acid (0.05 %), acetic acid (0.15 %), levulinic acid (0.25 %), furfural (7.2 mM), and ethanol (10 % v/v), respectively. Increased MSH content also decreased the concentration of reactive oxygen species in the presence of the above stresses. Our results may open a new avenue for enhancing robustness of industrial bacteria for production of commodity chemicals.
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, 712100, People's Rep of ublicChina.
authors.forename: Ying-Bao
authors.initials: YB
authors.lastname: Liu
authors.forename: Can
authors.initials: C
authors.lastname: Chen
authors.forename: Muhammad Tausif
authors.initials: MT
authors.lastname: Chaudhry
authors.forename: Mei-Ru
authors.initials: MR
authors.lastname: Si
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.forename: Yao
authors.initials: Y
authors.lastname: Wang
authors.forename: Xi-Hui
authors.initials: XH
authors.lastname: Shen
mesh_terms: D048230:Corynebacterium glutamicum / Q000187:drug effects / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D003545:Cysteine / Q000378:metabolism*; D015870:Gene Expression; D006020:Glycopeptides / Q000378:metabolism*; D016695:Glycosyltransferases / Q000235:genetics* / Q000378:metabolism*; D007294:Inositol / Q000378:metabolism*; D050296:Microbial Viability / Q000187:drug effects; D013312:Stress, Physiological
chemical_list: D006020:Glycopeptides; C089265:mycothiol; D007294:Inositol; D016695:Glycosyltransferases; D003545:Cysteine