title: Uptake of polymyxin B into renal cells.
abstract: Polymyxin B is increasingly used as a treatment of last resort against multidrug-resistant Gram-negative infections. Using a mammalian kidney cell line, we demonstrated that polymyxin B uptake into proximal tubular epithelial cells was saturable and occurred primarily through the apical membrane, suggesting the involvement of transporters in the renal uptake of polymyxin B. Megalin might play a role in the uptake and accumulation of polymyxin B into renal cells.
authors.affiliation: Department of Pharmacological and Pharmaceutical Sciences, University of Houston College of Pharmacy, Houston, Texas, USA.
authors.forename: Kamilia
authors.initials: K
authors.lastname: Abdelraouf
authors.affiliation: Department of Clinical Sciences and Administration, University of Houston College of Pharmacy, Houston, Texas, USA.
authors.forename: Kai-Tai
authors.initials: KT
authors.lastname: Chang
authors.affiliation: Department of Pharmacological and Pharmaceutical Sciences, University of Houston College of Pharmacy, Houston, Texas, USA.
authors.forename: Taijun
authors.initials: T
authors.lastname: Yin
authors.affiliation: Department of Pharmacological and Pharmaceutical Sciences, University of Houston College of Pharmacy, Houston, Texas, USA.
authors.forename: Ming
authors.initials: M
authors.lastname: Hu
authors.affiliation: Department of Pharmacological and Pharmaceutical Sciences, University of Houston College of Pharmacy, Houston, Texas, USA Department of Clinical Sciences and Administration, University of Houston College of Pharmacy, Houston, Texas, USA vtam@uh.edu.
authors.forename: Vincent H
authors.initials: VH
authors.lastname: Tam
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000378:metabolism* / Q000627:therapeutic use; D001693:Biological Transport, Active; D002460:Cell Line; D024901:Drug Resistance, Multiple, Bacterial; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D016905:Gram-Negative Bacterial Infections / Q000188:drug therapy / Q000382:microbiology; D007687:Kidney Tubules, Proximal / Q000166:cytology / Q000378:metabolism*; D026561:Low Density Lipoprotein Receptor-Related Protein-2 / Q000378:metabolism*; D011112:Polymyxin B / Q000378:metabolism* / Q000627:therapeutic use; D013552:Swine
chemical_list: D000900:Anti-Bacterial Agents; D026561:Low Density Lipoprotein Receptor-Related Protein-2; D011112:Polymyxin B
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