title: Development of LC-MS determination method and back-propagation ANN pharmacokinetic model of corynoxeine in rat.
abstract: Corynoxeine(CX), isolated from the extract of Uncaria rhynchophylla, is a useful and prospective compound in the prevention and treatment for vascular diseases. A simple and selective liquid chromatography mass spectrometry (LC-MS) method was developed to determine the concentration of CX in rat plasma. The chromatographic separation was achieved on a Zorbax SB-C18 (2.1 mm × 150 mm, 5 μm) column with acetonitrile-0.1% formic acid in water as mobile phase. Selective ion monitoring (SIM) mode was used for quantification using target ions m/z 383 for CX and m/z 237 for the carbamazepine (IS). After the LC-MS method was validated, it was applied to a back-propagation artificial neural network (BP-ANN) pharmacokinetic model study of CX in rats. The results showed that after intravenous administration of CX, it was mainly distributed in blood and eliminated quickly, t1/2 was less than 1h. The predicted concentrations generated by BP-ANN model had a high correlation coefficient (R>0.99) with experimental values. The developed BP-ANN pharmacokinetic model can be used to predict the concentration of CX in rats.
authors.affiliation: Function Experiment Teaching Center, Wenzhou Medical University, 325035, Wenzhou, China.
authors.forename: Jianshe
authors.initials: J
authors.lastname: Ma
authors.affiliation: The 2nd Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University, 325000, Wenzhou, China.
authors.forename: Jinzhang
authors.initials: J
authors.lastname: Cai
authors.affiliation: The First Affiliated Hospital of Wenzhou Medical University, 325035, Wenzhou, China.
authors.forename: Guanyang
authors.initials: G
authors.lastname: Lin
authors.affiliation: College of Physics and Electronic Information Engineering, Wenzhou University, 325035, Wenzhou, China.
authors.forename: Huilin
authors.initials: H
authors.lastname: Chen
authors.affiliation: Function Experiment Teaching Center, Wenzhou Medical University, 325035, Wenzhou, China.
authors.forename: Xianqin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Function Experiment Teaching Center, Wenzhou Medical University, 325035, Wenzhou, China.
authors.forename: Xianchuan
authors.initials: X
authors.lastname: Wang
authors.affiliation: The First Affiliated Hospital of Wenzhou Medical University, 325035, Wenzhou, China. Electronic address: hulufeng79@sina.com.
authors.forename: Lufeng
authors.initials: L
authors.lastname: Hu
mesh_terms: D000284:Administration, Oral; D000470:Alkaloids / Q000008:administration & dosage / Q000493:pharmacokinetics*; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000493:pharmacokinetics*; D026121:Indole Alkaloids; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D016571:Neural Networks, Computer; D051381:Rats; D017207:Rats, Sprague-Dawley; D032070:Uncaria / Q000737:chemistry*
chemical_list: D000470:Alkaloids; D004365:Drugs, Chinese Herbal; D026121:Indole Alkaloids; C054799:corynoxeine
keywords: BP-ANN; Corynoxeine; LC–MS; Pharmacokinetics