title: The IFN-λ Genetic Polymorphism Association With the Viral Clearance Induced by Hepatitis C Virus Treatment in Pakistani Patients.
abstract: BACKGROUND
  Polymorphisms in the interferon λ (INF λ) genes on chromosome 19 have been associated with clearance of hepatitis C virus (HCV) induced by interferon and ribavirin therapy however there is no such data available for Pakistani patients with HCV infection.
  
  
  OBJECTIVE
  In this study, the effects of single nucleotide polymorphisms (SNPs) have been investigated in response to treatment with interferon-α and ribavirin in a cohort of 75 HCV genotype 3a patients.
  
  
  METHODS
  A total number of 50 SNPs from the Interferon λ region on chromosome 19 were genotyped to investigate allelic associations with the treatment response in HCV type 3a patients. Thirteen SNPs were associated with HCV clearance, with the most significant alleles being RS8109886 (Fisher's P = 0.0001), RS8113007 (Fisher's P = 0.0001) and RS12979860 (Fisher's P = 0.0002).
  
  
  RESULTS
  These SNPs were found to be the most suitable SNPs for predicting treatment response in the present study. These findings support those reported previously. This could be used to improve HCV treatment strategies and suggest that Pakistani patients should be genotyped for the relevant SNPs to identify the patients who are more likely to respond to interferon and ribavirin therapy.
  
  
  CONCLUSIONS
  This therapy is costly and can be accompanied by several adverse side-effects, hence pre-treatment prediction of patients who are most likely to benefit would have both economic and patient benefits in the long term.
authors.affiliation: Institute of Biochemistry and Biotechnology, University of the Punjab, Lahore, Pakistan ; Manchester Institute of Biotechnology, University of Manchester, Manchester, UK.
authors.forename: Imran
authors.initials: I
authors.lastname: Tipu
authors.affiliation: Manchester Institute of Biotechnology, University of Manchester, Manchester, UK ; Centre for Integrated Genomic Medical Research, University of Manchester, Manchester, UK.
authors.forename: Fiona
authors.initials: F
authors.lastname: Marriage
authors.affiliation: Manchester Institute of Biotechnology, University of Manchester, Manchester, UK ; National University of Science and Technology, Islamabad, Pakistan.
authors.forename: Zia-Ur-Rahman
authors.initials: ZU
authors.lastname: Farooqi
authors.affiliation: Centre for Integrated Genomic Medical Research, University of Manchester, Manchester, UK.
authors.forename: Hazel
authors.initials: H
authors.lastname: Platt
authors.affiliation: Institute of Biochemistry and Biotechnology, University of the Punjab, Lahore, Pakistan.
authors.forename: Muhammad Amin
authors.initials: MA
authors.lastname: Athar
authors.affiliation: Manchester Institute of Biotechnology, University of Manchester, Manchester, UK ; Centre for Integrated Genomic Medical Research, University of Manchester, Manchester, UK.
authors.forename: Philip John
authors.initials: PJ
authors.lastname: Day
authors.affiliation: Centre for Integrated Genomic Medical Research, University of Manchester, Manchester, UK.
authors.forename: Andrea
authors.initials: A
authors.lastname: Short
keywords: Antiviral Agents; Hepacivirus; Interferons; Polymorphism, Genetic; Polymorphism, Single Nucleotide; Ribavirin
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