title: Antibiotic conjugated fluorescent carbon dots as a theranostic agent for controlled drug release, bioimaging, and enhanced antimicrobial activity.
abstract: A novel report on microwave assisted synthesis of bright carbon dots (C-dots) using gum arabic (GA) and its use as molecular vehicle to ferry ciprofloxacin hydrochloride, a broad spectrum antibiotic, is reported in the present work. Density gradient centrifugation (DGC) was used to separate different types of C-dots. After careful analysis of the fractions obtained after centrifugation, ciprofloxacin was attached to synthesize ciprofloxacin conjugated with C-dots (Cipro @ C-dots conjugate). Release of ciprofloxacin was found to be extremely regulated under physiological conditions. Cipro @ C-dots were found to be biocompatible on Vero cells as compared to free ciprofloxacin (1.2 mM) even at very high concentrations. Bare C-dots ( ∼ 13 mg mL(-1)) were used for microbial imaging of the simplest eukaryotic model-Saccharomyces cerevisiae (yeast). Bright green fluorescent was obtained when live imaging was performed to view yeast cells under fluorescent microscope suggesting C-dots incorporation inside the cells. Cipro @ C-dots conjugate also showed enhanced antimicrobial activity against both model gram positive and gram negative microorganisms. Thus, the Cipro @ C-dots conjugate paves not only a way for bioimaging but also an efficient new nanocarrier for controlled drug release with high antimicrobial activity, thereby serving potential tool for theranostics.
authors.affiliation: N.S.N. Research Center for Nanotechnology and Bionanotechnology, Ambernath, Maharashtra 421505, India.
authors.forename: Mukeshchand
authors.identifier: 0000-0003-4561-595X
authors.initials: M
authors.lastname: Thakur
authors.affiliation: N.S.N. Research Center for Nanotechnology and Bionanotechnology, Ambernath, Maharashtra 421505, India.
authors.forename: Sunil
authors.identifier: 0000-0003-0783-4764
authors.initials: S
authors.lastname: Pandey
authors.affiliation: N.S.N. Research Center for Nanotechnology and Bionanotechnology, Ambernath, Maharashtra 421505, India.
authors.forename: Ashmi
authors.identifier: 0000-0003-2140-0305
authors.initials: A
authors.lastname: Mewada
authors.affiliation: N.S.N. Research Center for Nanotechnology and Bionanotechnology, Ambernath, Maharashtra 421505, India.
authors.forename: Vaibhav
authors.initials: V
authors.lastname: Patil
authors.affiliation: N.S.N. Research Center for Nanotechnology and Bionanotechnology, Ambernath, Maharashtra 421505, India.
authors.forename: Monika
authors.initials: M
authors.lastname: Khade
authors.affiliation: N.S.N. Research Center for Nanotechnology and Bionanotechnology, Ambernath, Maharashtra 421505, India.
authors.forename: Ekta
authors.initials: E
authors.lastname: Goshi
authors.affiliation: N.S.N. Research Center for Nanotechnology and Bionanotechnology, Ambernath, Maharashtra 421505, India.
authors.forename: Madhuri
authors.initials: M
authors.lastname: Sharon
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