title: [Methodology of BMSCs in repairing ureteral injury in mice via renal artery transplantation].
abstract: OBJECTIVE
  To investigate the effect of bone marrow mesenchymal stem cells (BMSCs) on ureteral injury repair via renal artery transplantation.
  
  
  METHODS
  The left ureteral obstruction model was set up in 49 Balb/c mice by micro vascular clamp. The microscopic vascular clamp was taken out to lift the left ureteral obstruction after 10 days. The mice were randomly divided into an experimental group (n=25) and a control group (n=24). Balb/c mice BMSCs transfected by luciferase (Luc) were transplanted immediately through the renal artery after removing the microscopic vascular clamp from the experimental group; while mice in the control group was closed the incision after the microscopic vascular clamp was removed immediately and without BMSCs transplant. Magnetic resonance imaging (MRI) was used to scan the experimental mice. By measuring the left renal pelvis volume of the experimental mice at different time points and comparing the left ureter recanalization rate after removing left ureteral obstruction of the experimental group and the control group, we evaluated the repair effect of BMSCs on ureteral injury.
  
  
  RESULTS
  The volume of the left renal pelvis in experimental mice became bigger obviously after the left ureter was obstructed (P<0.01). The left renal pelvis volume of the experimental group and the control group had no statistical significance 10 days after the left ureteral obstruction was set up (P=0.693). In the experimental group, the left ureter recanalization rate was higher than that in the control group, after removing the left ureteral obstruction (P=0.012).
  
  
  CONCLUSIONS
  Transplantation through the renal artery can promote the restoration of ureteral injury in mice.
authors.affiliation: Department of Urology, Affiliated Haikou Hospital, Xiangya School of Medicine, Central South University, Haikou 570208, China.
authors.forename: Donghui
authors.initials: D
authors.lastname: Li
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Bai
authors.forename: Jindong
authors.initials: J
authors.lastname: Li
authors.forename: Qing
authors.initials: Q
authors.lastname: Cheng
authors.forename: Ziyi
authors.initials: Z
authors.lastname: Guo
authors.forename: Xiangdong
authors.initials: X
authors.lastname: Deng
authors.forename: Hui
authors.initials: H
authors.lastname: Cao
mesh_terms: D000818:Animals; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D006412:Hematopoietic Stem Cells / Q000166:cytology; D051379:Mice; D008807:Mice, Inbred BALB C; D012077:Renal Artery; D014517:Ureteral Obstruction / Q000628:therapy*