title: Development of a method to recovery and amplification DNA by real-time PCR from commercial vegetable oils.
abstract: This study describes the design of a suitable DNA isolation method from commercial vegetable oils for the application of DNA markers for food safety and traceability. Firstly, a comparative study was made of eight methods for the recovery of high quality DNA from olive, sunflower and palm oils, and a CTAB-based method was selected. In order to optimize this method, the effect of the organic compounds and several components in the lysis buffer and the lysis and precipitation time were evaluated. For the purpose of overcoming the limitations detected in spectrophotometric and PCR DNA yield evaluations, the performance of the extraction protocols during the optimization processes was evaluated using qPCR. The suggested DNA extraction optimized is less time consuming than other conventional DNA extraction methods, uses a reduced oil volume and is cheaper than available commercial kits. Additionally, the applicability of this method has been successfully assayed in ten commercial vegetable oils and derivatives.
authors.affiliation: Department of Biotechnology and Food Science, University of Burgos, Plaza Misael Bañuelos, s/n, 09001 Burgos, Spain.
authors.forename: Sonia
authors.initials: S
authors.lastname: Ramos-Gómez
authors.affiliation: Department of Biotechnology and Food Science, University of Burgos, Plaza Misael Bañuelos, s/n, 09001 Burgos, Spain.
authors.forename: María D
authors.initials: MD
authors.lastname: Busto
authors.affiliation: Department of Biotechnology and Food Science, University of Burgos, Plaza Misael Bañuelos, s/n, 09001 Burgos, Spain.
authors.forename: Manuel
authors.initials: M
authors.lastname: Perez-Mateos
authors.affiliation: Department of Biotechnology and Food Science, University of Burgos, Plaza Misael Bañuelos, s/n, 09001 Burgos, Spain. Electronic address: nortega@ubu.es.
authors.forename: Natividad
authors.initials: N
authors.lastname: Ortega
mesh_terms: D021141:Nucleic Acid Amplification Techniques; D010938:Plant Oils / Q000032:analysis*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*
chemical_list: D010938:Plant Oils
keywords: CTAB; Commercial vegetable oil; DNA detection; DNA extraction; qPCR