title: Mucosal adjuvant activity of IL-2 presenting spores of bacillus subtilis in a murine model of Helicobacter pylori vaccination.
abstract: The endospores of Bacillus subtilis are now widely used as a platform for presentation of heterologous proteins and due to their safety record and high resistance to harsh environmental conditions can be considered as potential vehicles for oral vaccination. In this research we show that recombinant B. subtilis spores presenting a fragment of the Helicobacter acinonychis UreB protein and expressing the ureB gene under vegetative promoter elicit a strong cellular immune response in orally immunized mice when co-administered with spores presenting IL-2. We show for the first time the successful application of two types of recombinant spores, one carrying an antigen and the other an adjuvant, in a single oral immunization.
authors.affiliation: Department of Medical Biotechnology, Intercollegiate Faculty of Biotechnology UG-MUG, Medical University of Gdańsk, Gdańsk, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Hinc
authors.affiliation: Department of Medical Biotechnology, Intercollegiate Faculty of Biotechnology UG-MUG, University of Gdańsk, Gdańsk, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Stasiłojć
authors.affiliation: Department of Medical Biotechnology, Intercollegiate Faculty of Biotechnology UG-MUG, University of Gdańsk, Gdańsk, Poland.
authors.forename: Iwona
authors.initials: I
authors.lastname: Piątek
authors.affiliation: Tri-City Animal Laboratory, Medical University of Gdańsk, Gdańsk, Poland.
authors.forename: Grażyna
authors.initials: G
authors.lastname: Peszyńska-Sularz
authors.affiliation: Department of Biology, Federico II University of Naples, Naples, Italy.
authors.forename: Rachele
authors.initials: R
authors.lastname: Isticato
authors.affiliation: Department of Biology, Federico II University of Naples, Naples, Italy.
authors.forename: Ezio
authors.initials: E
authors.lastname: Ricca
authors.affiliation: Department of Medical Biotechnology, Intercollegiate Faculty of Biotechnology UG-MUG, Medical University of Gdańsk, Gdańsk, Poland.
authors.forename: Michał
authors.initials: M
authors.lastname: Obuchowski
authors.affiliation: Department of Medical Biotechnology, Intercollegiate Faculty of Biotechnology UG-MUG, Medical University of Gdańsk, Gdańsk, Poland.
authors.forename: Adam
authors.initials: A
authors.lastname: Iwanicki
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D001412:Bacillus subtilis / Q000502:physiology*; D001428:Bacterial Vaccines / Q000235:genetics / Q000276:immunology* / Q000382:microbiology; D005260:Female; D016481:Helicobacter Infections / Q000235:genetics / Q000276:immunology / Q000378:metabolism / Q000517:prevention & control*; D016480:Helicobacter pylori / Q000276:immunology*; D007376:Interleukin-2 / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D013171:Spores, Bacterial / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D014611:Vaccination
chemical_list: D000276:Adjuvants, Immunologic; D001428:Bacterial Vaccines; D007376:Interleukin-2
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