title: Differential effects of chemoattractants on mast cell recruitment in vivo.
abstract: Rats were injected with rat recombinant (rr) IL3, rrSCF, rrIL-3+rrSCF, rrRANTES and LTB4. Six hours after subcutaneous injection of rrIL-3 or rrIL-3+rrSCF, there was an increase in mast cell numbers in the skin and spleen. Peritoneal mast cells were recruited following i.p. injection of rrIL-3, but with rrIL-3+rrSCF recruitment was delayed. Immunostaining with a mast cell specific antibody showed that immature orthochromatic mast cells were being recruited. rrIL-3 induced recruitment of mast cells to the peritoneal cavity was blocked by anti-integrin antibodies. Mast cell recruitment depended on the target tissue and the time of exposure to the chemoattractant.
authors.affiliation: Department of Cell and Molecular Biology and Pathogenic Bioagents, Ribeirão Preto Medical School, University of São Paulo, Avenida Bandeirantes 3900, 14049-900 Ribeirão Preto, SP, Brazil.
authors.forename: Maria Rita
authors.initials: MR
authors.lastname: de Cássia Campos
authors.affiliation: Department of Cell and Molecular Biology and Pathogenic Bioagents, Ribeirão Preto Medical School, University of São Paulo, Avenida Bandeirantes 3900, 14049-900 Ribeirão Preto, SP, Brazil.
authors.forename: Vanina Danuza
authors.initials: VD
authors.lastname: Toso
authors.affiliation: Department of Cell and Molecular Biology and Pathogenic Bioagents, Ribeirão Preto Medical School, University of São Paulo, Avenida Bandeirantes 3900, 14049-900 Ribeirão Preto, SP, Brazil.
authors.forename: Devandir Antonio
authors.initials: DA
authors.lastname: de Souza
authors.affiliation: Department of Cell and Molecular Biology and Pathogenic Bioagents, Ribeirão Preto Medical School, University of São Paulo, Avenida Bandeirantes 3900, 14049-900 Ribeirão Preto, SP, Brazil.
authors.forename: Gabriel Viliod
authors.initials: GV
authors.lastname: Vieira
authors.affiliation: Department of Cell and Molecular Biology and Pathogenic Bioagents, Ribeirão Preto Medical School, University of São Paulo, Avenida Bandeirantes 3900, 14049-900 Ribeirão Preto, SP, Brazil.
authors.forename: Elaine Zayas Marcelino
authors.initials: EZ
authors.lastname: da Silva
authors.affiliation: Department of Cell and Molecular Biology and Pathogenic Bioagents, Ribeirão Preto Medical School, University of São Paulo, Avenida Bandeirantes 3900, 14049-900 Ribeirão Preto, SP, Brazil.
authors.forename: Constance
authors.initials: C
authors.lastname: Oliver
authors.affiliation: Department of Cell and Molecular Biology and Pathogenic Bioagents, Ribeirão Preto Medical School, University of São Paulo, Avenida Bandeirantes 3900, 14049-900 Ribeirão Preto, SP, Brazil. Electronic address: mjamur@fmrp.usp.br.
authors.forename: Maria Célia
authors.initials: MC
authors.lastname: Jamur
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000276:immunology; D018946:Chemokine CCL5 / Q000494:pharmacology; D002630:Chemotactic Factors / Q000276:immunology* / Q000494:pharmacology; D007377:Interleukin-3 / Q000494:pharmacology; D007975:Leukotriene B4 / Q000494:pharmacology; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000276:immunology*; D010529:Peritoneal Cavity; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000494:pharmacology; D012867:Skin / Q000166:cytology / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology; D019089:Stem Cell Factor / Q000494:pharmacology
chemical_list: D018946:Chemokine CCL5; D002630:Chemotactic Factors; D007377:Interleukin-3; D011994:Recombinant Proteins; D019089:Stem Cell Factor; D007975:Leukotriene B4
keywords: IL-3; In vivo; LTB4; Mast cells; RANTES; Recruitment; SCF