title: [Growth inhibitory effect of microRNA-519b-3p on larynx squamous Hep-2 cells].
abstract: OBJECTIVE
  To investigate the effects of microRNA-519b-3p (miR-519b-3p) on laryngeal carcinoma Hep-2 cell growth, and to analyze the underlying molecular mechanisms.
  
  
  METHODS
  The effects of miR-519b-3p on the growth and cell cycle of Hep-2 cells transfected with miR-519b-3p mimic were tested by MTT assay and flow cytometry, respectively. The mRNA and protein expressions of the related genes were tested by reverse transcription polymerase chain reaction (RT-PCR) and Western blot, respectively. The expressions of miR-519b-3p were tested by real-time RT-PCR in 48 pairs of laryngeal carcinoma and adjacent tissue samples.
  
  
  RESULTS
  The expression of miR-519b-3p in laryngeal carcinoma tissues was significant lower than that in adjacent non-cancerous tissues (S(ΔCt) = 2.989, t = 2.693, P < 0.01) . Increasing the level of miR-519b-3p inhibited significantly Hep-2 cell proliferation, arrested the cell cycle in the G2/M phase (10.29% ± 4.63%, t = 4.395, P < 0.05) , and decreased significantly the percentage of cells in the S phase (7.56% ± 2.05%, t = 3.555, P < 0.05) , with the increase in the expression of cyclin dependent kinase (CDK) 1 and the decrease in the expressions of CDK 2 and Cyclin A. RT-PCR and Western blot showed that miR-519b-3p down-regulated the protein but not mRNA expressions of HuR and cyclooxygenase-2(COX-2) genes.
  
  
  CONCLUSIONS
  The expression of MiR-519b-3p as carcinoma suppressor gene is low in laryngeal carcinoma. The cell cycle of Hep-2 cells was arrested in the G2/M phase by MiR-519b-3p.
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Ningbo Medical Treatment Center Lihuili Hospital, Ningbo 315040, China. Email: szs7216@163.com.
authors.forename: Zhisen
authors.initials: Z
authors.lastname: Shen
authors.forename: Guowen
authors.initials: G
authors.lastname: Zhan
authors.forename: Hongxia
authors.initials: H
authors.lastname: Deng
authors.forename: Cheng
authors.initials: C
authors.lastname: Kang
authors.affiliation: Medical School of Ningbo University, Zhejiang Provincial Key Laboratory of Pathophysiology, Ningbo 315211, China. Email: junmingguo@yahoo.com.
authors.forename: Junming
authors.initials: J
authors.lastname: Guo
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics / Q000473:pathology*; D035683:MicroRNAs / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D014162:Transfection
chemical_list: C546194:MIRN519 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger