title: Corneal wound healing promoted by 3 blood derivatives: an in vitro and in vivo comparative study.
abstract: OBJECTIVE
  The aim of this study was to compare the effect on corneal wound healing of 3 differently manufactured blood derivatives [autologous serum (AS), platelet-rich plasma, and serum derived from plasma rich in growth factors (s-PRGF)].
  
  
  METHODS
  Scratch wound-healing assays were performed on rabbit primary corneal epithelial cultures and human corneal epithelial cells. Additionally, mechanical debridement of rabbit corneal epithelium was performed. Wound-healing progression was assessed by measuring the denuded areas remaining over time after treatment with each of the 3 blood derivatives or a control treatment.
  
  
  RESULTS
  In vitro data show statistically significant differences in the healing process with all the derivatives compared with the control, but 2 of them (AS and s-PRGF) induced markedly faster wound healing. In contrast, although the mean time required to complete in vivo reepithelization was similar to that of AS and s-PRGF treatment, only wounds treated with s-PRGF were significantly smaller in size from 2.5 days onward with respect to the control treatment.
  
  
  CONCLUSIONS
  All 3 blood derivatives studied are promoters of corneal reepithelization. However, the corneal wound-healing progresses differently with each derivative, being faster in vitro under AS and s-PRGF treatment and producing in vivo the greatest decrease in wound size under s-PRGF treatment. These findings highlight that the manufacturing process of the blood derivatives may modulate the efficacy of the final product.
authors.affiliation: *Department of Cell Biology and Histology, School of Medicine and Dentistry, BioCruces Health Research Institute, University of the Basque Country, Leioa, Spain; †Department of R&D, Instituto Clínico-Quirúrgico de Oftalmología, Bilbao, Spain; ‡BioCruces Health Research Institute, Cruces University Hospital, Barakaldo, Spain; and §Department of Ophthalmology, School of Medicine and Dentistry, BioCruces Health Research Institute, University of the Basque Country, Leioa, Spain.
authors.forename: Vanesa
authors.initials: V
authors.lastname: Freire
authors.forename: Noelia
authors.initials: N
authors.lastname: Andollo
authors.forename: Jaime
authors.initials: J
authors.lastname: Etxebarria
authors.forename: Raquel
authors.initials: R
authors.lastname: Hernáez-Moya
authors.forename: Juan A
authors.initials: JA
authors.lastname: Durán
authors.forename: María-Celia
authors.initials: MC
authors.lastname: Morales
mesh_terms: D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D004195:Disease Models, Animal; D019573:Epithelium, Corneal / Q000293:injuries*; D005260:Female; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000502:physiology*; D009883:Ophthalmic Solutions; D053657:Platelet-Rich Plasma / Q000502:physiology; D011817:Rabbits; D044967:Serum / Q000502:physiology; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D009883:Ophthalmic Solutions