title: A genome-wide screen for sporulation-defective mutants in Schizosaccharomyces pombe.
abstract: Yeast sporulation is a highly regulated developmental program by which diploid cells generate haploid gametes, termed spores. To better define the genetic pathways regulating sporulation, a systematic screen of the set of ~3300 nonessential Schizosaccharomyces pombe gene deletion mutants was performed to identify genes required for spore formation. A high-throughput genetic method was used to introduce each mutant into an h(90) background, and iodine staining was used to identify sporulation-defective mutants. The screen identified 34 genes whose deletion reduces sporulation, including 15 that are defective in forespore membrane morphogenesis. In S. pombe, the total number of sporulation-defective mutants is a significantly smaller fraction of coding genes than in S. cerevisiae, which reflects the different evolutionary histories and biology of the two yeasts.
authors.affiliation: Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, New York 11794-5215.
authors.forename: Esma
authors.initials: E
authors.lastname: Ucisik-Akkaya
authors.affiliation: Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, New York 11794-5222.
authors.forename: Janet K
authors.initials: JK
authors.lastname: Leatherwood
authors.affiliation: Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, New York 11794-5215 aaron.neiman@stonybrook.edu.
authors.forename: Aaron M
authors.initials: AM
authors.lastname: Neiman
mesh_terms: D015966:Gene Expression Regulation, Fungal; D055106:Genome-Wide Association Study; D006238:Haploidy; D008540:Meiosis / Q000235:genetics; D009154:Mutation; D010641:Phenotype; D012568:Schizosaccharomyces / Q000235:genetics*; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics*; D017384:Sequence Deletion; D013172:Spores, Fungal / Q000235:genetics*
chemical_list: D029702:Schizosaccharomyces pombe Proteins
keywords: erp2; erp5; forespore membrane; knockout collection
references.citation: J Cell Sci. 2002 Jan 15;115(Pt 2):409-20
references.pmid: 11839791
references.citation: J Cell Sci. 2004 Jan 26;117(Pt 3):389-96
references.pmid: 14702385
references.citation: Mol Cell Biol. 2002 Jan;22(1):309-20
references.pmid: 11739743
references.citation: Eukaryot Cell. 2006 Nov;5(11):1866-81
references.pmid: 16963626
references.citation: Nature. 1997 Jun 12;387(6634):708-13
references.pmid: 9192896
references.citation: Nat Biotechnol. 2010 Jun;28(6):617-623
references.pmid: 20473289
references.citation: Genes Cells. 2007 Feb;12(2):155-70
references.pmid: 17295836
references.citation: J Cell Biol. 1998 Jan 12;140(1):29-37
references.pmid: 9425151
references.citation: FEBS Lett. 2000 Jul 28;478(1-2):105-8
references.pmid: 10922478
references.citation: Methods Mol Biol. 2009;557:65-76
references.pmid: 19799177
references.citation: J Cell Sci. 2000 Feb;113 ( Pt 3):545-54
references.pmid: 10639340
references.citation: Mol Biol Cell. 2008 Jun;19(6):2476-87
references.pmid: 18367542
references.citation: J Cell Sci. 2009 Dec 1;122(Pt 23):4330-40
references.pmid: 19887589
references.citation: Genetics. 2000 Jan;154(1):147-54
references.pmid: 10628976
references.citation: Cell. 1994 Aug 12;78(3):487-98
references.pmid: 7520368
references.citation: Yeast. 2006 Feb;23(3):173-83
references.pmid: 16498704
references.citation: Biol Cell. 2009 Sep;101(9):495-509
references.pmid: 19566487
references.citation: Nature. 1988 Apr 7;332(6164):509-14
references.pmid: 3357510
references.citation: Curr Biol. 1999 Dec 2;9(23):1427-30
references.pmid: 10607571
references.citation: Proc Natl Acad Sci U S A. 2002 Nov 26;99(24):15530-5
references.pmid: 12432101
references.citation: J Cell Sci. 2003 Jan 15;116(Pt 2):259-71
references.pmid: 12482912
references.citation: Biosci Biotechnol Biochem. 2007 Dec;71(12):3041-7
references.pmid: 18071249
references.citation: Mol Cell Biol. 2004 Sep;24(17):7402-18
references.pmid: 15314152
references.citation: Mol Biol Cell. 2003 Mar;14(3):1109-24
references.pmid: 12631727
references.citation: Mol Gen Genet. 1997 Jun;255(2):226-36
references.pmid: 9236781
references.citation: Mol Cell Biol. 1997 Dec;17(12):7008-18
references.pmid: 9372932
references.citation: Science. 2004 Feb 27;303(5662):1367-70
references.pmid: 14752166
references.citation: Trends Genet. 2000 Sep;16(9):395-403
references.pmid: 10973068
references.citation: Nucleic Acids Res. 1993 Jun 25;21(12):2955-6
references.pmid: 8332516
references.citation: Genetics. 2011 Feb;187(2):385-96
references.pmid: 21098718
references.citation: EMBO J. 1991 Dec;10(12):3743-51
references.pmid: 1657593
references.citation: J Cell Sci. 1982 Aug;56:263-79
references.pmid: 7166567
references.citation: Mol Microbiol. 2006 Feb;59(3):836-53
references.pmid: 16420355
references.citation: Genetics. 2003 Jan;163(1):47-54
references.pmid: 12586695
references.citation: Mol Biol Cell. 2002 Feb;13(2):493-502
references.pmid: 11854407
references.citation: Arch Mikrobiol. 1973 Apr 8;91(1):1-10
references.pmid: 4711455
references.citation: Curr Genet. 1986;10(6):443-7
references.pmid: 3442824
references.citation: J Bacteriol. 1978 Apr;134(1):208-13
references.pmid: 348678
references.citation: EMBO J. 2002 Jun 3;21(11):2807-18
references.pmid: 12032093
references.citation: Genetics. 2014 Apr;196(4):1059-76
references.pmid: 24514900
references.citation: Arch Microbiol. 1977 Feb 4;112(1):109-10
references.pmid: 843164
references.citation: Curr Biol. 2005 Sep 20;15(18):1663-9
references.pmid: 16169489
references.citation: Eukaryot Cell. 2006 Aug;5(8):1301-13
references.pmid: 16896214
references.citation: Science. 2001 Dec 14;294(5550):2364-8
references.pmid: 11743205
references.citation: Biosci Biotechnol Biochem. 2009 Feb;73(2):339-45
references.pmid: 19202289
references.citation: Proc Natl Acad Sci U S A. 2002 Aug 6;99(16):10330-4
references.pmid: 12149471
references.citation: Mol Cell Biol. 2010 Apr;30(8):2057-74
references.pmid: 20123972
references.citation: PLoS Genet. 2013;9(8):e1003715
references.pmid: 23950735
references.citation: Biosci Biotechnol Biochem. 2002 Oct;66(10):2224-7
references.pmid: 12450137
references.citation: Nucleic Acids Res. 2012 Jan;40(Database issue):D695-9
references.pmid: 22039153
references.citation: Mol Cell Biol. 1998 Apr;18(4):2118-29
references.pmid: 9528784
references.citation: EMBO J. 2005 Nov 16;24(22):3940-51
references.pmid: 16252005
references.citation: Mol Biol Cell. 2011 Sep;22(18):3442-55
references.pmid: 21775631
references.citation: J Biol Chem. 2013 Dec 27;288(52):37057-70
references.pmid: 24217251
references.citation: Mol Biol Cell. 2001 Apr;12(4):901-17
references.pmid: 11294895
references.citation: Microbiol Mol Biol Rev. 2005 Dec;69(4):565-84
references.pmid: 16339736
references.citation: Mol Biol Cell. 2011 Oct;22(19):3658-70
references.pmid: 21832151
references.citation: Methods Enzymol. 2010;470:145-79
references.pmid: 20946810
references.citation: PLoS One. 2013 Jul 17;8(7):e69765
references.pmid: 23874994
references.citation: Biotechniques. 2011 Jul;51(1):57-60
references.pmid: 21781055
references.citation: Mol Biol Cell. 2001 Dec;12(12):3955-72
references.pmid: 11739793
references.citation: Microbiol Rev. 1985 Mar;49(1):81-99
references.pmid: 3157043
references.citation: Nature. 2005 Mar 24;434(7032):529-33
references.pmid: 15791259
references.citation: Genetics. 1975 Aug;80(4):711-4
references.pmid: 1193372
references.citation: Mol Biol Cell. 2003 Dec;14(12):4971-83
references.pmid: 12960419
references.citation: Genetics. 1989 May;122(1):19-27
references.pmid: 2659436
references.citation: PLoS Genet. 2013;9(8):e1003700
references.pmid: 23966878
references.citation: J Cell Biol. 1998 Sep 7;142(5):1209-22
references.pmid: 9732282
references.citation: Methods Enzymol. 2010;470:759-95
references.pmid: 20946835
references.citation: Cell Struct Funct. 2005;30(2):15-24
references.pmid: 16272747
references.citation: Curr Biol. 2005 Nov 22;15(22):2056-62
references.pmid: 16303567
references.citation: EMBO J. 1988 Mar;7(3):761-7
references.pmid: 2840284
references.citation: Mol Gen Genet. 1968;102(4):301-6
references.pmid: 5743433
references.citation: J Cell Sci. 2007 Mar 1;120(Pt 5):908-16
references.pmid: 17298976
references.citation: Eukaryot Cell. 2005 Jan;4(1):166-77
references.pmid: 15643072