title: Association between serum levels of carotenoids and serum asymmetric dimethylarginine levels in Japanese subjects.
abstract: BACKGROUND
  Asymmetric dimethylarginine (ADMA) is an endogenous inhibitor of endothelium nitric oxide synthase (NOS). ADMA binds to a substrate-binding site of NOS and then inhibits nitric oxide production from vascular endothelial cells. Elevated ADMA levels are a risk factor for cardiovascular disease. Recently, it was reported that plasma ADMA levels were negatively correlated with vegetable and fruit consumption. The purpose of this study was to examine the association between serum levels of carotenoids and serum ADMA levels in Japanese subjects.
  
  
  METHODS
  We conducted a cross-sectional study of 470 subjects (203 men and 267 women) who attended a health examination in August 2011. Serum levels of several carotenoids were separately measured by high-performance liquid chromatography. Serum ADMA levels were determined by using an enzyme-linked immunosorbent assay kit.
  
  
  RESULTS
  In women, the multivariate-adjusted odds ratios (ORs) of elevated serum ADMA levels were significantly decreased in the highest tertile for β-cryptoxanthin (OR 0.47, 95% CI 0.23-0.95), α-carotene (OR 0.39, 95% CI 0.18-0.79), and β-carotene (OR 0.36, 95% CI 0.17-0.73) compared to the lowest tertile. In men, significantly decreased ORs were observed in the highest tertiles of serum zeaxanthin/lutein (OR 0.23, 95% CI 0.06-0.69) and α-carotene (OR 0.26, 95% CI 0.07-0.82), and in the middle and the highest tertiles of serum β-carotene (OR 0.27, 95% CI 0.09-0.74 and OR 0.20, 95% CI 0.03-0.88, respectively) when the tertile cutoff points of women were extrapolated to men.
  
  
  CONCLUSIONS
  Higher serum levels of carotenoids, such as α-carotene and β-carotene, may help to prevent elevated serum ADMA levels in Japanese subjects.
authors.affiliation: Clinical Laboratory Medicine, Fujita Health University Graduate School of Health Sciences.
authors.forename: Rika
authors.initials: R
authors.lastname: Watarai
authors.forename: Koji
authors.initials: K
authors.lastname: Suzuki
authors.forename: Naohiro
authors.initials: N
authors.lastname: Ichino
authors.forename: Keisuke
authors.initials: K
authors.lastname: Osakabe
authors.forename: Keiko
authors.initials: K
authors.lastname: Sugimoto
authors.forename: Hiroya
authors.initials: H
authors.lastname: Yamada
authors.forename: Takeshi
authors.initials: T
authors.lastname: Hamajima
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Hamajima
authors.forename: Takashi
authors.initials: T
authors.lastname: Inoue
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001120:Arginine / Q000031:analogs & derivatives* / Q000097:blood; D002338:Carotenoids / Q000097:blood*; D003430:Cross-Sectional Studies; D005260:Female; D006801:Humans; D007564:Japan; D008297:Male; D008875:Middle Aged; D019207:beta Carotene / Q000097:blood*
chemical_list: C487735:dimethylarginine; D019207:beta Carotene; D002338:Carotenoids; C041635:alpha-carotene; D001120:Arginine
references.citation: Metabolism. 2012 Aug;61(8):1171-6
references.pmid: 22386943
references.citation: Clin Chem. 2004 Mar;50(3):582-8
references.pmid: 14726472
references.citation: J Diabetes Investig. 2012 Feb 20;3(1):39-40
references.pmid: 24843544
references.citation: Annu Rev Med. 1997;48:489-509
references.pmid: 9046979
references.citation: J Am Coll Cardiol. 2005 Aug 2;46(3):518-23
references.pmid: 16053968
references.citation: Eur J Epidemiol. 2004;19(10):915-21
references.pmid: 15575349
references.citation: Clin Nutr. 2003 Feb;22(1):17-22
references.pmid: 12553945
references.citation: N Engl J Med. 1996 May 2;334(18):1145-9
references.pmid: 8602179
references.citation: Jpn J Cancer Res. 1987 Oct;78(10):1049-56
references.pmid: 3119536
references.citation: Am Heart J. 2006 Sep;152(3):493.e1-8
references.pmid: 16923419
references.citation: Clin Investig. 1993 Jan;71(1):3-6
references.pmid: 8453256
references.citation: Arterioscler Thromb Vasc Biol. 2003 Aug 1;23(8):1455-9
references.pmid: 12805079
references.citation: Curr Atheroscler Rep. 2003 Nov;5(6):492-9
references.pmid: 14525683
references.citation: Clin Chim Acta. 1990 Dec 24;194(2-3):131-44
references.pmid: 2128764
references.citation: Circulation. 1999 Mar 9;99(9):1141-6
references.pmid: 10069780
references.citation: Ann Med. 1995 Jun;27(3):343-51
references.pmid: 7546624
references.citation: Gend Med. 2008 Sep;5(3):218-28
references.pmid: 18727988
references.citation: J Clin Hypertens (Greenwich). 2006 Dec;8(12 Suppl 4):17-29
references.pmid: 17170603
references.citation: JAMA. 2002 Mar 20;287(11):1420-6
references.pmid: 11903029
references.citation: Circulation. 1998 Nov 3;98(18):1842-7
references.pmid: 9799202
references.citation: Free Radic Biol Med. 1999 Apr;26(7-8):1034-53
references.pmid: 10232849
references.citation: Circulation. 2002 Aug 20;106(8):987-92
references.pmid: 12186805
references.citation: Hypertens Res. 2009 Jan;32(1):3-107
references.pmid: 19300436
references.citation: Circulation. 2009 Mar 31;119(12):1592-600
references.pmid: 19289633
references.citation: Vascul Pharmacol. 2008 Oct-Dec;49(4-6):134-40
references.pmid: 18692595
references.citation: N Engl J Med. 1996 May 2;334(18):1150-5
references.pmid: 8602180
references.citation: Vasc Med. 2005 Jul;10 Suppl 1:S73-81
references.pmid: 16444872
references.citation: Nutrition. 2001 Oct;17(10):815-7
references.pmid: 11684386
references.citation: Circulation. 2003 Apr 15;107(14):1891-5
references.pmid: 12681993
references.citation: Lancet. 1992 Mar 7;339(8793):572-5
references.pmid: 1347093
references.citation: Cancer Epidemiol Biomarkers Prev. 1994 Sep;3(6):493-500
references.pmid: 8000300
references.citation: JAMA. 1994 Nov 9;272(18):1439-41
references.pmid: 7933426
references.citation: J Epidemiol. 2006 Jul;16(4):154-60
references.pmid: 16837766
references.citation: Circulation. 1999 Jun 22;99(24):3092-5
references.pmid: 10377069
references.citation: Rev Diabet Stud. 2010 Spring;7(1):15-25
references.pmid: 20703435
references.citation: J Atheroscler Thromb. 2007 Apr;:5-57
references.pmid: 17566346