title: New improved method for fructooligosaccharides production by Aureobasidium pullulans.
abstract: Fructooligosaccharides are prebiotics with numerous health benefits within which the improvement of gut microbiota balance can be highlighted, playing a key role in individual health. In this study, an integrated one-stage method for FOS production via sucrose fermentation by Aureobasidium pullulans was developed and optimized using experimental design tools. Optimization of temperature and agitation speed for maximizing the FOS production was performed using response surface methodology. Temperature was found to be the most significant parameter. The optimum fermentation conditions were found to be 32 °C and 385 rpm. Under these conditions, the model predicted a total FOS production yield of 64.7 gFOS/gsucrose. The model was validated at optimal conditions in order to check its adequacy and accuracy and an experimental yield of 64.1 (±0.0) gFOS/gsucrose was obtained. A significant improvement of the total FOS production yields by A. pullulans using a one-stage process was obtained.
authors.affiliation: IBB - Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Campus de Gualtar, 4710-057 Braga, Portugal.
authors.forename: Ana
authors.initials: A
authors.lastname: Dominguez
authors.affiliation: IBB - Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Campus de Gualtar, 4710-057 Braga, Portugal.
authors.forename: Clarisse
authors.initials: C
authors.lastname: Nobre
authors.affiliation: IBB - Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Campus de Gualtar, 4710-057 Braga, Portugal; Biotempo - Consultoria em Biotecnologia, Lda., Avepark - Edifício Spinpark, Zona Industrial da Gandra, Barco, 4805-017 Guimarães, Portugal.
authors.forename: Lígia R
authors.initials: LR
authors.lastname: Rodrigues
authors.affiliation: LSRE - Laboratory of Separation and Reaction Engineering - Associate Laboratory LSRE/LCM, Escola Superior Agrária, Instituto Politécnico de Bragança, Quinta de Santa Apolónia, Apartado 172, 5301-855 Bragança, Portugal.
authors.forename: António M
authors.initials: AM
authors.lastname: Peres
authors.affiliation: IBB - Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Campus de Gualtar, 4710-057 Braga, Portugal; Biotempo - Consultoria em Biotecnologia, Lda., Avepark - Edifício Spinpark, Zona Industrial da Gandra, Barco, 4805-017 Guimarães, Portugal.
authors.forename: Duarte
authors.initials: D
authors.lastname: Torres
authors.affiliation: IBB - Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Campus de Gualtar, 4710-057 Braga, Portugal; Biotempo - Consultoria em Biotecnologia, Lda., Avepark - Edifício Spinpark, Zona Industrial da Gandra, Barco, 4805-017 Guimarães, Portugal.
authors.forename: Isabel
authors.initials: I
authors.lastname: Rocha
authors.affiliation: IBB - Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Campus de Gualtar, 4710-057 Braga, Portugal.
authors.forename: Nelson
authors.initials: N
authors.lastname: Lima
authors.affiliation: IBB - Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Campus de Gualtar, 4710-057 Braga, Portugal.
authors.forename: José
authors.initials: J
authors.lastname: Teixeira
mesh_terms: D001203:Ascomycota / Q000254:growth & development*; D062610:Fungal Polysaccharides / Q000096:biosynthesis* / Q000737:chemistry; D009844:Oligosaccharides / Q000096:biosynthesis* / Q000737:chemistry; D013395:Sucrose / Q000494:pharmacology; D013549:Sweetening Agents / Q000494:pharmacology
chemical_list: D062610:Fungal Polysaccharides; D009844:Oligosaccharides; D013549:Sweetening Agents; C116580:fructooligosaccharide; D013395:Sucrose