title: DNA copy number changes in tumors within the spectrum of cellular, atypical, and metastasizing fibrous histiocytoma.
abstract: BACKGROUND
  Cutaneous fibrous histiocytoma (FH) is a common mesenchymal neoplasm. Metastasis is rare, disproportionately occurring among the aneurysmal, cellular, atypical, and deep variants.
  
  
  OBJECTIVE
  We determined whether DNA copy number changes occurred in atypical FH (AFH), and whether they were similar to those in metastasizing FH (MetFH) and benign cellular FH (CFH).
  
  
  METHODS
  Five primary tumors of MetFH were evaluated by array-based comparative genomic hybridization analysis, with tissue from local recurrences and lung metastases in 2 and 2 patients, respectively. Seven indolent AFH and 5 CFH were identified for comparison.
  
  
  RESULTS
  Substantial differences between the groups were found both in the frequency of chromosomal aberrations (higher among MetFH and absent or solitary in CFH) and array-based comparative genomic hybridization profiles (frequent gains of 7 and 8q and losses of Xq in MetFH; recurrent losses of chromosomes 9 and 22 in AFH; isolated loss of 5q and gain in chromosome 20 in 2 CFH). Fatal MetFH cases (2 of 5 cases) exhibited the highest rate of chromosomal aberrations.
  
  
  CONCLUSIONS
  This study included a small sample size with a short-term follow-up.
  
  
  CONCLUSIONS
  Benign CFH, indolent AFH, and MetFH represent distinct biological entities within the spectrum of FH; array-based comparative genomic hybridization may be a tool in recognizing FH cases with metastatic potential and increasingly aggressive behavior.
authors.affiliation: Pathology Department, University of California, San Francisco, California; Dermatology Department, University of California, San Francisco, California.
authors.forename: Yann
authors.initials: Y
authors.lastname: Charli-Joseph
authors.affiliation: Pathology Department, University of California, San Francisco, California; Dermatology Department, University of California, San Francisco, California.
authors.forename: Andrea
authors.initials: A
authors.lastname: Saggini
authors.affiliation: Pathology Department, Brigham and Women's Hospital, Boston, Massachusetts.
authors.forename: Leona A
authors.initials: LA
authors.lastname: Doyle
authors.affiliation: Pathology Department, Brigham and Women's Hospital, Boston, Massachusetts.
authors.forename: Christopher D
authors.initials: CD
authors.lastname: Fletcher
authors.affiliation: Pathology Department, University of California, San Francisco, California.
authors.forename: Jingly
authors.initials: J
authors.lastname: Weier
authors.affiliation: Pathology Department, University of California, San Francisco, California.
authors.forename: Sonia
authors.initials: S
authors.lastname: Mirza
authors.affiliation: Pathology Department, University of California, San Francisco, California.
authors.forename: Swapna
authors.initials: S
authors.lastname: Vemula
authors.affiliation: Pathology Department, University of California, San Francisco, California; Dermatology Department, University of California, San Francisco, California; Helen A. Diller Family Comprehensive Cancer Center, University of California, San Francisco, California. Electronic address: philip.leboit@ucsf.edu.
authors.forename: Philip E
authors.initials: PE
authors.lastname: LeBoit
mesh_terms: D000328:Adult; D000368:Aged; D002872:Chromosome Deletion; D002890:Chromosomes, Human, Pair 20; D002892:Chromosomes, Human, Pair 22; D002899:Chromosomes, Human, Pair 9; D055028:Comparative Genomic Hybridization; D056915:DNA Copy Number Variations; D005260:Female; D018219:Histiocytoma, Benign Fibrous / Q000235:genetics* / Q000473:pathology; D051677:Histiocytoma, Malignant Fibrous / Q000235:genetics* / Q000473:pathology / Q000556:secondary; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000556:secondary; D008297:Male; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000235:genetics* / Q000473:pathology; D012878:Skin Neoplasms / Q000235:genetics* / Q000473:pathology*; D055815:Young Adult
keywords: array-based comparative genomic hybridization; atypical fibrous histiocytoma; cellular fibrous histiocytoma; fibrous histiocytoma; genomic instability; metastasizing fibrous histiocytoma