title: Transglutaminase 2 on the surface of dendritic cells is proposed to be involved in dendritic cell-T cell interaction.
abstract: Transglutaminase 2 (TG2) is a ubiquitous enzyme involved in diverse biological processes. Recently, its function in adaptive immune responses has begun to emerge. Its presence and functions in B cells and T cells, for example, have been reported. However, those in dendritic cells (DCs), the principal antigen-presenting cells, are as yet unexplored in murine system. In this study, we first investigated the expression of TG2 in murine bone marrow-derived DCs, and then compared the functioning of these cells in the presence or absence of this enzyme using wild-type (WT) and TG2(-/-) mice. We found that the WT DCs expressed TG2 both in the cytoplasm and on the cell surface, both of which were elevated after LPS stimulation. Unexpectedly, between WT and TG2(-/-) DCs, there were no remarkable differences in cytokine secretion, IL-10 and IL-12, and neither in the expression of surface molecules CD80, CD86, and MHC II, excepting a moderate decrease of CD40 expression on the TG2(-/-) DCs. However, when T cells were stimulated with TG2(-/-) DCs, they showed decreased levels of proliferation, CD69 and CD25 expression, and IFN-γ secretion. The addition of anti-TG2 antibody to the WT DC-T cell co-culture resulted in decreased T cell activation. By immunofluorescence staining, TG2 was observed at DC-T cell interface (contact point). Taken together, we propose that TG2 on the surface of DCs modulates the DC-T cell interaction.
authors.affiliation: Department of Anatomy, Seoul National University College of Medicine, Seoul 110-799, Republic of Korea.
authors.forename: Jin-Hee
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Department of Biochemistry and Molecular Biology, Seoul National University College of Medicine, Seoul 110-799, Republic of Korea.
authors.forename: Eui Man
authors.initials: EM
authors.lastname: Jeong
authors.affiliation: Department of Anatomy, Seoul National University College of Medicine, Seoul 110-799, Republic of Korea.
authors.forename: Young-Joo
authors.initials: YJ
authors.lastname: Jeong
authors.affiliation: Department of Anatomy, Seoul National University College of Medicine, Seoul 110-799, Republic of Korea.
authors.forename: Wang Jae
authors.initials: WJ
authors.lastname: Lee
authors.affiliation: Department of Anatomy, Seoul National University College of Medicine, Seoul 110-799, Republic of Korea.
authors.forename: Jae Seung
authors.initials: JS
authors.lastname: Kang
authors.affiliation: Department of Biochemistry and Molecular Biology, Seoul National University College of Medicine, Seoul 110-799, Republic of Korea.
authors.forename: In-Gyu
authors.initials: IG
authors.lastname: Kim
authors.affiliation: Department of Anatomy, Seoul National University College of Medicine, Seoul 110-799, Republic of Korea. Electronic address: hyi830@snu.ac.kr.
authors.forename: Young-il
authors.initials: YI
authors.lastname: Hwang
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D015703:Antigens, CD / Q000096:biosynthesis; D000945:Antigens, Differentiation, T-Lymphocyte / Q000096:biosynthesis; D018122:B7-1 Antigen / Q000096:biosynthesis; D051940:B7-2 Antigen / Q000096:biosynthesis; D001854:Bone Marrow Cells / Q000166:cytology / Q000276:immunology; D019013:CD40 Antigens / Q000096:biosynthesis; D002450:Cell Communication / Q000276:immunology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000201:enzymology / Q000276:immunology*; D019204:GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis; D007371:Interferon-gamma / Q000378:metabolism; D016753:Interleukin-10 / Q000378:metabolism; D018664:Interleukin-12 / Q000378:metabolism; D053645:Interleukin-2 Receptor alpha Subunit / Q000096:biosynthesis; D037181:Lectins, C-Type / Q000096:biosynthesis; D008213:Lymphocyte Activation / Q000276:immunology*; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D000091345:Protein Glutamine gamma Glutamyltransferase 2; D013601:T-Lymphocytes / Q000276:immunology*; D011503:Transglutaminases / Q000235:genetics / Q000378:metabolism*; D000099192:CD69 Antigens
chemical_list: D000906:Antibodies; D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D018122:B7-1 Antigen; D051940:B7-2 Antigen; D019013:CD40 Antigens; D019204:GTP-Binding Proteins; D000949:Histocompatibility Antigens Class II; D007371:Interferon-gamma; D016753:Interleukin-10; D018664:Interleukin-12; D053645:Interleukin-2 Receptor alpha Subunit; D037181:Lectins, C-Type; D000091345:Protein Glutamine gamma Glutamyltransferase 2; D011503:Transglutaminases; D000099192:CD69 Antigens; C497965:Cd86 protein, mouse; C556062:IL10 protein, mouse; C508018:Il2ra protein, mouse
keywords: DC–T cell interaction; Dendritic cell; T cell activation; Transglutaminase 2