title: Conversion of female germline stem cells from neonatal and prepubertal mice into pluripotent stem cells.
abstract: Pluripotent stem cells derived from neonatal or adult testes are a useful tool to examine the mechanisms of pluripotency and a resource for cell-based therapies. However, therapies using these cells will only benefit males but not females. Recently, female germline stem cells (FGSCs) were discovered in ovaries. Whether FGSCs can be converted into pluripotent stem cells, similar to spermatogonial stem cells, is unknown. Here, we demonstrate that female embryonic stem-like cells (fESLCs) can be generated within 1 month from the stably proliferating FGSCs cultured in embryonic stem cell (ESC) medium. fESLCs exhibit properties similar to those of ESCs in terms of marker expression and differentiation potential. Thus, our findings suggest that generation of patient-specific fESLCs is feasible and provides a foundation for personalized regenerative applications.
authors.affiliation: Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education), Bio-X Institutes, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Hu
authors.initials: H
authors.lastname: Wang
authors.forename: Manxi
authors.initials: M
authors.lastname: Jiang
authors.forename: Haiwei
authors.initials: H
authors.lastname: Bi
authors.forename: Xuejin
authors.initials: X
authors.lastname: Chen
authors.forename: Lin
authors.initials: L
authors.lastname: He
authors.forename: Xiaoyong
authors.initials: X
authors.lastname: Li
authors.forename: Ji
authors.initials: J
authors.lastname: Wu
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002454:Cell Differentiation; D002678:Chimera; D019175:DNA Methylation / Q000235:genetics; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D005260:Female; D018392:Genomic Imprinting; D005854:Germ Cells / Q000166:cytology* / Q000378:metabolism; D008810:Mice, Inbred C57BL; D010641:Phenotype; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D011627:Puberty / Q000378:metabolism*
keywords: adult stem cells; embryonic stem-like cells; female germline stem cells