title: Filamentous fungi in microtiter plates-an easy way to optimize itaconic acid production with Aspergillus terreus.
abstract: Itaconic acid is an important industrial building block and is produced by the filamentous fungi Aspergillus terreus. To make the optimization process more efficient, a scale-down from shake flasks to microtiter plates was performed. This resulted in comparable product formations, and 87.7 g/L itaconic acid was formed after 10 days of cultivation in the microtiter plate. The components of the minimal medium were varied independently for a media optimization. This resulted in an increase of the itaconic acid concentration by a variation of the KH2PO4 and CuSO4 concentrations. The cultivation with a higher KH2PO4 concentration in a 400-mL bioreactor showed an increase in the maximum productivity of 1.88 g/L/h, which was an increase of 74 % in comparison to the reference. Neither the phosphate concentration nor the nitrogen sources were limited at the start of the product formation. This showed that a limitation of these substances is not necessary for the itaconic acid formation.
authors.affiliation: Thünen-Institute of Agricultural Technology, Bundesallee 50, 38116, Braunschweig, Germany.
authors.forename: Antje
authors.initials: A
authors.lastname: Hevekerl
authors.forename: Anja
authors.initials: A
authors.lastname: Kuenz
authors.forename: Klaus-Dieter
authors.initials: KD
authors.lastname: Vorlop
mesh_terms: D001230:Aspergillus / Q000378:metabolism*; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D003470:Culture Media / Q000737:chemistry*; D005285:Fermentation; D005658:Fungi / Q000378:metabolism*; D009584:Nitrogen / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism; D013386:Succinates / Q000378:metabolism*; D013431:Sulfates / Q000378:metabolism
chemical_list: D003470:Culture Media; D010710:Phosphates; D013386:Succinates; D013431:Sulfates; D009584:Nitrogen; C005229:itaconic acid