title: The pattern of change in the abundances of specific bacterioplankton groups is consistent across different nutrient-enriched habitats in Crete.
abstract: A common source of disturbance for coastal aquatic habitats is nutrient enrichment through anthropogenic activities. Although the water column bacterioplankton communities in these environments have been characterized in some cases, changes in α-diversity and/or the abundances of specific taxonomic groups across enriched habitats remain unclear. Here, we investigated the bacterial community changes at three different nutrient-enriched and adjacent undisturbed habitats along the north coast of Crete, Greece: a fish farm, a closed bay within a town with low water renewal rates, and a city port where the level of nutrient enrichment and the trophic status of the habitat were different. Even though changes in α-diversity were different at each site, we observed across the sites a common change pattern accounting for most of the community variation for five of the most abundant bacterial groups: a decrease in the abundance of the Pelagibacteraceae and SAR86 and an increase in the abundance of the Alteromonadaceae, Rhodobacteraceae, and Cryomorphaceae in the impacted sites. The abundances of the groups that increased and decreased in the impacted sites were significantly correlated (positively and negatively, respectively) with the total heterotrophic bacterial counts and the concentrations of dissolved organic carbon and/or dissolved nitrogen and chlorophyll α, indicating that the common change pattern was associated with nutrient enrichment. Our results provide an in situ indication concerning the association of specific bacterioplankton groups with nutrient enrichment. These groups could potentially be used as indicators for nutrient enrichment if the pattern is confirmed over a broader spatial and temporal scale by future studies.
authors.affiliation: Department of Biology, University of Crete, Heraklion, Crete, Greece.
authors.forename: Stilianos
authors.initials: S
authors.lastname: Fodelianakis
authors.affiliation: Department of Biology, University of Crete, Heraklion, Crete, Greece.
authors.forename: Nafsika
authors.initials: N
authors.lastname: Papageorgiou
authors.affiliation: Hellenic Center for Marine Research, Heraklion, Crete, Greece.
authors.forename: Paraskevi
authors.initials: P
authors.lastname: Pitta
authors.affiliation: Hellenic Center for Marine Research, Heraklion, Crete, Greece.
authors.forename: Panagiotis
authors.initials: P
authors.lastname: Kasapidis
authors.affiliation: Department of Biology, University of Crete, Heraklion, Crete, Greece.
authors.forename: Ioannis
authors.initials: I
authors.lastname: Karakassis
authors.affiliation: Department of Biology, University of Crete, Heraklion, Crete, Greece ladoukakis@biology.uoc.gr.
authors.forename: Emmanuel D
authors.initials: ED
authors.lastname: Ladoukakis
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000235:genetics*; D058448:Biota; D002734:Chlorophyll / Q000032:analysis; D000077194:Chlorophyll A; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D017753:Ecosystem; D006115:Greece; D008969:Molecular Sequence Data; D017672:Nitrogen Compounds / Q000032:analysis; D009930:Organic Chemicals / Q000032:analysis; D012623:Seawater / Q000737:chemistry / Q000382:microbiology*; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D017672:Nitrogen Compounds; D009930:Organic Chemicals; D002734:Chlorophyll; D000077194:Chlorophyll A
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