title: Myxidium ceccarellii n. sp. (Myxosporea) from the gallbladder of Leporinus elongatus (Anastomidae) from the São Francisco River, Brazil.
abstract: During a survey of myxozoan parasites of freshwater fish from the São Francisco River in Minas Gerais State, Brazil, plasmodia of Myxidium ceccarellii n. sp. were found in gallbladders of two out of six specimens (22%) of Leporinus elongatus (Anastomidae). Parasite plasmodia were translucent and greenish, with disporic sporoblasts that develop asynchronously. Mature myxospores were ellipsoidal in frontal and lateral views, with slightly pointed ends. The surfaces of each valve had four to six longitudinal grooves. Spores dimensions were as follows: length 17.7 ± 0.5 μm (17.1-18.1), width 10.4 ± 0.47 μm (9.8-11.3), and thickness 10.1 ± 0.27 μm (9.6-10.4). Two polar capsules, one at either end of the spore, had the length of 6.3 ± 0.5 μm (5.7-7.0) and width of 6.4 ± 0.44 μm (5.7-6.9), with four to five polar filament turns. Some aberrant spores had one or three polar capsules. Partial sequencing of M. ceccarellii n. sp. small subunit ribosomal RNA (ssrRNA) gene resulted in 1,845 bp. This is the first molecular study of a Myxidium species that parasitizes a South American freshwater fish. Phylogenetic reconstruction using ssrRNA gene sequences showed that M. ceccarellii n. sp. was positioned basally in a recognized clade of myxozoans that infect the biliary systems of nonfish vertebrates.
authors.affiliation: Departamento de Ciências Biológicas, Universidade Federal de São Paulo (UNIFESP), Rua Professor Artur Riedel, 275, Jardim Eldorado, 09972-270, Diadema, SP, Brazil, edapadriano@gmail.com.
authors.forename: Edson A
authors.initials: EA
authors.lastname: Adriano
authors.forename: Marcia R M
authors.initials: MR
authors.lastname: Silva
authors.forename: Stephen D
authors.initials: SD
authors.lastname: Atkinson
authors.forename: Jerri L
authors.initials: JL
authors.lastname: Bartholomew
authors.forename: Antônio A M
authors.initials: AA
authors.lastname: Maia
mesh_terms: D000818:Animals; D001938:Brazil; D060125:Characiformes / Q000469:parasitology*; D005393:Fish Diseases / Q000469:parasitology*; D005704:Gallbladder / Q000469:parasitology*; D055764:Myxozoa / Q000648:ultrastructure; D010273:Parasitic Diseases, Animal / Q000469:parasitology*; D010802:Phylogeny; D012335:RNA, Ribosomal / Q000145:classification* / Q000235:genetics; D054679:Ribosome Subunits, Small / Q000235:genetics; D045483:Rivers; D017422:Sequence Analysis, DNA; D013170:Spores / Q000235:genetics / Q000648:ultrastructure
chemical_list: D012335:RNA, Ribosomal
references.citation: Braz J Biol. 2003 May;63(2):253-60
references.pmid: 14509847
references.citation: Syst Parasitol. 2011 Oct;80(2):81-116
references.pmid: 21898199
references.citation: Mol Biol Evol. 2008 Jul;25(7):1253-6
references.pmid: 18397919
references.citation: Parasitol Res. 2012 Jan;110(1):211-8
references.pmid: 21674297
references.citation: J Parasitol. 1997 Apr;83(2):262-71
references.pmid: 9105308
references.citation: PLoS One. 2011 Apr 25;6(4):e18871
references.pmid: 21541340
references.citation: J Parasitol. 2006 Jun;92(3):611-9
references.pmid: 16884007
references.citation: Mem Inst Oswaldo Cruz. 2011 Aug;106(5):557-61
references.pmid: 21894376
references.citation: Int J Parasitol. 2008 Aug;38(10):1199-207
references.pmid: 18342316
references.citation: Nucleic Acids Res. 1997 Sep 1;25(17):3389-402
references.pmid: 9254694
references.citation: Dis Aquat Organ. 2001 Oct 8;46(3):197-212
references.pmid: 11710554
references.citation: J Parasitol. 2000 Feb;86(1):103-11
references.pmid: 10701572
references.citation: Syst Parasitol. 2014 Jan;87(1):47-72
references.pmid: 24395575
references.citation: Parasitology. 2012 Apr;139(4):478-96
references.pmid: 22260881
references.citation: Int J Parasitol. 2006 Dec;36(14):1521-34
references.pmid: 16904677
references.citation: Syst Biol. 2010 May;59(3):307-21
references.pmid: 20525638