title: Phosphorescent quantum dots/doxorubicin nanohybrids based on photoinduced electron transfer for detection of DNA.
abstract: MPA-capped Mn-doped ZnS QDs/DXR nanohybrids (MPA: 3-mercaptopropionic acid; QDs: quantum dots; DXR: cetyltrimethyl ammonium bromide) were constructed via photoinduced electron transfer (PIET) and then used as a room-temperature phosphorescence (RTP) probe for detection of DNA. DXR as a quencher will quench the RTP of Mn-doped ZnS QDs via PIET, thereby forming Mn-doped ZnS QDs/DXR nanohybrids and storing RTP. With the addition of DNA, it will be inserted into DXR and thus DXR will be competitively desorbed from the surface of Mn-doped ZnS QDs, thereby releasing the RTP of Mn-doped ZnS QDs. Based on this, a new method for DNA detection was built. The sensor for DNA has a detection limit of 0.039 mg L(-1) and a linear range from 0.1 to 14 mg L(-1). The present QDs-based RTP method does not need deoxidants or other inducers as required by conventional RTP detection methods, and avoids interference from autofluorescence and the scattering light of the matrix that are encountered in spectrofluorometry. Therefore, this method can be used to detect the DNA content in body fluid.
authors.affiliation: Shanxi Normal University, Linfen 041004, PR China.
authors.forename: Yanming
authors.initials: Y
authors.lastname: Miao
authors.affiliation: Shanxi Normal University, Linfen 041004, PR China.
authors.forename: Zhifeng
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Shanxi Normal University, Linfen 041004, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Gong
authors.affiliation: Shanxi Normal University, Linfen 041004, PR China. Electronic address: gqyan2013@163.com.
authors.forename: Guiqin
authors.initials: G
authors.lastname: Yan
mesh_terms: D015097:3-Mercaptopropionic Acid / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry; D004247:DNA / Q000032:analysis / Q000652:urine*; D004317:Doxorubicin / Q000737:chemistry*; D004579:Electron Transport; D006801:Humans; D057230:Limit of Detection; D049408:Luminescent Agents / Q000737:chemistry*; D008163:Luminescent Measurements / Q000379:methods; D008345:Manganese / Q000737:chemistry; D045663:Quantum Dots / Q000737:chemistry*; D013440:Sulfides / Q000737:chemistry; D017967:Zinc Compounds / Q000737:chemistry
chemical_list: D002593:Cetrimonium Compounds; D049408:Luminescent Agents; D013440:Sulfides; D017967:Zinc Compounds; D008345:Manganese; D004317:Doxorubicin; D004247:DNA; D015097:3-Mercaptopropionic Acid; C031238:zinc sulfide; D000077286:Cetrimonium
keywords: DNA; Nanohybrids; Photoinduced electron transfer; Room-temperature phosphorescence