title: Microscopy, culture, and quantitative real-time PCR examination confirm internalization of mycobacteria in plants.
abstract: The environment is a reservoir of nontuberculous mycobacteria and is considered a source of infection for animals and humans. Mycobacteria can persist in different types of environments for a relatively long time. We have studied their possible internalization into plant tissue through intact, as well as damaged, root systems of different types of plants grown in vitro and under field conditions. The substrate into which plants were seeded was previously contaminated with different strains of Mycobacterium avium (10(8) to 10(10) cells/g of soil) and feces from animals with paratuberculosis. We detected M. avium subsp. avium, hominissuis, and paratuberculosis in the stems and leaves of the plants by both culture and real-time quantitative PCR. The presence of mycobacteria in the plant tissues was confirmed by microscopy. The concentration of mycobacteria found inside plant tissue was several orders of magnitude lower (up to 10(4) cells/g of tissue) than the initial concentration of mycobacteria present in the culture medium or substrate. These findings led us to the hypothesis that plants may play a role in the spread and transmission of mycobacteria to other organisms in the environment.
authors.affiliation: Veterinary Research Institute, Brno, Czech Republic kaevska@vri.cz.
authors.forename: M
authors.initials: M
authors.lastname: Kaevska
authors.affiliation: Department of Plant Biology, Mendel University in Brno, Brno, Czech Republic.
authors.forename: S
authors.initials: S
authors.lastname: Lvoncik
authors.affiliation: Veterinary Research Institute, Brno, Czech Republic.
authors.forename: I
authors.initials: I
authors.lastname: Slana
authors.affiliation: Veterinary Research Institute, Brno, Czech Republic.
authors.forename: P
authors.initials: P
authors.lastname: Kulich
authors.affiliation: Veterinary Research Institute, Brno, Czech Republic.
authors.forename: P
authors.initials: P
authors.lastname: Kralik
mesh_terms: D001431:Bacteriological Techniques; D004705:Endocytosis; D008853:Microscopy; D009161:Mycobacterium / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D018515:Plant Leaves / Q000382:microbiology; D018547:Plant Stems / Q000382:microbiology; D010944:Plants / Q000382:microbiology*; D060888:Real-Time Polymerase Chain Reaction
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