title: New insights into the roles of Xin repeat-containing proteins in cardiac development, function, and disease.
abstract: Since the discovery of Xin repeat-containing proteins in 1996, the importance of Xin proteins in muscle development, function, regeneration, and disease has been continuously implicated. Most Xin proteins are localized to myotendinous junctions of the skeletal muscle and also to intercalated discs (ICDs) of the heart. The Xin gene is only found in vertebrates, which are characterized by a true chambered heart. This suggests that the evolutionary origin of the Xin gene may have played a key role in vertebrate origins. Diverse vertebrates including mammals possess two paralogous genes, Xinα (or Xirp1) and Xinβ (or Xirp2), and this review focuses on the role of their encoded proteins in cardiac muscles. Complete loss of mouse Xinβ (mXinβ) results in the failure of forming ICD, severe growth retardation, and early postnatal lethality. Deletion of mouse Xinα (mXinα) leads to late-onset cardiomyopathy with conduction defects. Molecular studies have identified three classes of mXinα-interacting proteins: catenins, actin regulators/modulators, and ion-channel subunits. Thus, mXinα acts as a scaffolding protein modulating the N-cadherin-mediated adhesion and ion-channel surface expression. Xin expression is significantly upregulated in early stages of stressed hearts, whereas Xin expression is downregulated in failing hearts from various human cardiomyopathies. Thus, mutations in these Xin loci may lead to diverse cardiomyopathies and heart failure.
authors.affiliation: Department of Biology, University of Iowa, Iowa City, Iowa, USA.
authors.forename: Qinchuan
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Biology, University of Iowa, Iowa City, Iowa, USA.
authors.forename: Jenny Li-Chun
authors.initials: JL
authors.lastname: Lin
authors.affiliation: Department of Biology, University of Iowa, Iowa City, Iowa, USA.
authors.forename: Albert J
authors.initials: AJ
authors.lastname: Erives
authors.affiliation: Institute of Physiology, National Defense Medical Center, Taipei, Taiwan, ROC.
authors.forename: Cheng-I
authors.initials: CI
authors.lastname: Lin
authors.affiliation: Department of Biology, University of Iowa, Iowa City, Iowa, USA. Electronic address: jim-lin@uiowa.edu.
authors.forename: Jim Jung-Ching
authors.initials: JJ
authors.lastname: Lin
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D009202:Cardiomyopathies / Q000378:metabolism; D051356:Cortactin / Q000378:metabolism; D003598:Cytoskeletal Proteins; D003599:Cytoskeleton / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D015536:Down-Regulation; D005786:Gene Expression Regulation; D006321:Heart / Q000502:physiology; D006333:Heart Failure / Q000378:metabolism; D006801:Humans; D060588:LIM Domain Proteins / Q000378:metabolism*; D051379:Mice; D009154:Mutation; D009206:Myocardium / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism*; D015221:Potassium Channels / Q000378:metabolism; D017434:Protein Structure, Tertiary; D013997:Time Factors; D051176:beta Catenin / Q000378:metabolism
chemical_list: D000199:Actins; C572128:CMYA1 protein, mouse; D051356:Cortactin; D003598:Cytoskeletal Proteins; D004268:DNA-Binding Proteins; D060588:LIM Domain Proteins; D009687:Nuclear Proteins; D015221:Potassium Channels; C500305:XIRP2 protein, mouse; D051176:beta Catenin
keywords: Cardiomyopathy with conduction defect; Cortactin; Delayed K(+) rectifier current (I(k,slow1)); Intercalated disc formation; KChIP2; Transient K(+) outward current (I(to,f)); Xirp; β-Catenin
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