title: Fluorescence resonance energy transfer based immunosensing of human IgG by using quantum dot/GIgG-gold nanoparticles/IgG conjugation.
abstract: A novel immunosensor of human immune globulin (IgG) was fabricated based on the fluorescence transfer between luminescent semiconductor quantum dots (QDs) and gold nanoparticles (AuNPs). AuNPs and CdSe/ZnS QDs were respectively labeled with immune reaction pair:IgG and goat anti-human immunoglobulin (GIgG), by optimizing the conditions including pH value and protein amount. In the assembled QD-GIgG-IgG-AuNP fluorescence resonance energy transfer (FRET) immunocomplex system, the presence of AuNP-IgG directly reduced the fluorescence intensity of the GIgG conjugated QDs. As a result, the concentration of AuNP-IgG had a linear relationship with the fluorescence decrease in a range of 0-1.57 microg/mL. Furthermore, the mechanism of the QDs' fluorescence decay has also been discussed and attributed to the light-induced photobleaching. This novel sensing method achieves quantitative detection of trace proteins, suggesting the potential of biomolecule-AuNPs conjugation based analytical methods in further application.
authors.forename: Lin
authors.initials: L
authors.lastname: Luo
authors.forename: Zhao
authors.initials: Z
authors.lastname: Liu
authors.forename: Jianjun
authors.initials: J
authors.lastname: Li
authors.forename: Jian
authors.initials: J
authors.lastname: Zhu
mesh_terms: D000818:Animals; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D015200:Fluoroimmunoassay / Q000379:methods*; D006041:Goats; D006046:Gold / Q000737:chemistry*; D007074:Immunoglobulin G / Q000737:chemistry / Q000276:immunology*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D058726:Nanoconjugates / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D045663:Quantum Dots
chemical_list: D007074:Immunoglobulin G; D058726:Nanoconjugates; D006046:Gold