title: Diversity of immunoglobulin lambda light chain gene usage over developmental stages in the horse.
abstract: To further studies of neonatal immune responses to pathogens and vaccination, we investigated the dynamics of B lymphocyte development and immunoglobulin (Ig) gene diversity. Previously we demonstrated that equine fetal Ig VDJ sequences exhibit combinatorial and junctional diversity levels comparable to those of adult Ig VDJ sequences. Herein, RACE clones from fetal, neonatal, foal, and adult lymphoid tissue were assessed for Ig lambda light chain combinatorial, junctional, and sequence diversity. Remarkably, more lambda variable genes (IGLV) were used during fetal life than later stages and IGLV gene usage differed significantly with time, in contrast to the Ig heavy chain. Junctional diversity measured by CDR3L length was constant over time. Comparison of Ig lambda transcripts to germline revealed significant increases in nucleotide diversity over time, even during fetal life. These results suggest that the Ig lambda light chain provides an additional dimension of diversity to the equine Ig repertoire.
authors.affiliation: Equine Immunology Laboratory, Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, United States. Electronic address: rlt8@cornell.edu.
authors.forename: Rebecca L
authors.initials: RL
authors.lastname: Tallmadge
authors.affiliation: Equine Immunology Laboratory, Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, United States.
authors.forename: Chia T
authors.initials: CT
authors.lastname: Tseng
authors.affiliation: Equine Immunology Laboratory, Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, United States.
authors.forename: M Julia B
authors.initials: MJ
authors.lastname: Felippe
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D001402:B-Lymphocytes / Q000502:physiology; D005333:Fetus / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D006736:Horses / Q000235:genetics* / Q000378:metabolism; D007146:Immunoglobulin lambda-Chains / Q000235:genetics* / Q000378:metabolism; D058977:Molecular Sequence Annotation; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D060152:V(D)J Recombination
chemical_list: D007146:Immunoglobulin lambda-Chains
keywords: Development; Diversity; Equine; Immunoglobulin; Lambda; Light chain
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