title: Functional consequences of splicing of the antisense transcript COOLAIR on FLC transcription.
abstract: Antisense transcription is widespread in many genomes; however, how much is functional is hotly debated. We are investigating functionality of a set of long noncoding antisense transcripts, collectively called COOLAIR, produced at Arabidopsis FLOWERING LOCUS C (FLC). COOLAIR initiates just downstream of the major sense transcript poly(A) site and terminates either early or extends into the FLC promoter region. We now show that splicing of COOLAIR is functionally important. This was revealed through analysis of a hypomorphic mutation in the core spliceosome component PRP8. The prp8 mutation perturbs a cotranscriptional feedback mechanism linking COOLAIR processing to FLC gene body histone demethylation and reduced FLC transcription. The importance of COOLAIR splicing in this repression mechanism was confirmed by disrupting COOLAIR production and mutating the COOLAIR proximal splice acceptor site. Our findings suggest that altered splicing of a long noncoding transcript can quantitatively modulate gene expression through cotranscriptional coupling mechanisms.
authors.affiliation: Department of Cell & Developmental Biology, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Marquardt
authors.affiliation: Department of Cell & Developmental Biology, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK.
authors.forename: Oleg
authors.initials: O
authors.lastname: Raitskin
authors.affiliation: Department of Cell & Developmental Biology, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Department of Cell & Developmental Biology, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK.
authors.forename: Fuquan
authors.initials: F
authors.lastname: Liu
authors.affiliation: Department of Cell & Developmental Biology, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK.
authors.forename: Qianwen
authors.initials: Q
authors.lastname: Sun
authors.affiliation: Department of Cell & Developmental Biology, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK. Electronic address: caroline.dean@jic.ac.uk.
authors.forename: Caroline
authors.initials: C
authors.lastname: Dean
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D003640:Dealkylation; D025461:Feedback, Physiological; D035264:Flowers / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D006657:Histones / Q000378:metabolism; D026161:MADS Domain Proteins / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D012326:RNA Splicing; D016372:RNA, Antisense / Q000378:metabolism*; D062085:RNA, Long Noncoding / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D036226:Seedlings / Q000235:genetics / Q000378:metabolism; D013997:Time Factors; D014158:Transcription, Genetic
chemical_list: D029681:Arabidopsis Proteins; C418902:At5g10140 protein, Arabidopsis; D006657:Histones; D026161:MADS Domain Proteins; D016372:RNA, Antisense; D062085:RNA, Long Noncoding; D016601:RNA-Binding Proteins
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