title: Bradykinin postconditioning ameliorates focal cerebral ischemia in the rat.
abstract: The goal of this study is to investigate the effects of bradykinin (BR) postconditioning on cerebral ischemic injury. Transient focal cerebral ischemia was induced in rats by 60min of middle cerebral artery occlusion (MCAO), followed by 3days of reperfusion. BR as a postconditioner at a dose of 150μg/kg was applied intraperitoneally 3, 6, 24 and 48h after MCAO. BR postconditioning significantly reduced total infarct volumes if applied 3h after MCAO by 95%, 6h after MCAO by 80% and 24h after MCAO by 70% in versus vehicle group. Neurological functions were amarked improvement in the BR groups compared to the ischemia group. The number of degenerated neurons in the hippocampal CA1 region was also significantly lower in BR-treated ischemic groups compared to vehicle group. BR postconditioning prevented the release of MnSOD from the mitochondria and reduced the activity of the total SOD and CAT if it is administrated short time after stroke. Our data proves the ischemic tolerance in the brain induced by BR postconditioning resulted as effective agent against as strong an attack as 60min MCAO even when used many hours after ischemia.
authors.affiliation: Institute of Neurobiology, Slovak Academy of Sciences, Kosice, Slovakia. Electronic address: danielis@saske.sk.
authors.forename: Viera
authors.initials: V
authors.lastname: Danielisova
authors.affiliation: Institute of Neurobiology, Slovak Academy of Sciences, Kosice, Slovakia.
authors.forename: Miroslav
authors.initials: M
authors.lastname: Gottlieb
authors.affiliation: Institute of Neurobiology, Slovak Academy of Sciences, Kosice, Slovakia.
authors.forename: Petra
authors.initials: P
authors.lastname: Bonova
authors.affiliation: Institute of Neurobiology, Slovak Academy of Sciences, Kosice, Slovakia.
authors.forename: Miroslava
authors.initials: M
authors.lastname: Nemethova
authors.affiliation: Institute of Neurobiology, Slovak Academy of Sciences, Kosice, Slovakia.
authors.forename: Jozef
authors.initials: J
authors.lastname: Burda
mesh_terms: D000818:Animals; D034961:Antigens, Nuclear / Q000378:metabolism; D001920:Bradykinin / Q000494:pharmacology*; D002545:Brain Ischemia / Q000188:drug therapy* / Q000473:pathology; D056547:CA1 Region, Hippocampal / Q000187:drug effects / Q000473:pathology; D002374:Catalase / Q000378:metabolism; D002544:Cerebral Infarction / Q000188:drug therapy / Q000473:pathology; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism; D057775:Ischemic Postconditioning / Q000379:methods*; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D034961:Antigens, Nuclear; D009419:Nerve Tissue Proteins; C546339:Rbfox3 protein, rat; D002374:Catalase; D013482:Superoxide Dismutase; D001920:Bradykinin
keywords: Bradykinin; Cerebral ischemia; Endogenous antioxidant enzymes; Hippocampus; Postconditioning