title: Isolation and antitumor activities of acidic polysaccharide from Gynostemma pentaphyllum Makino.
abstract: Two acidic polysaccharides (GP-B1 and GP-C1) were obtained from Gynostemma pentaphyllum. The molecular weights (Mw) of the two fractions were 79 kDa for GP-B1 and 126 kDa for GP-C1. GP-B1 was composed of Gal, Ara, Man, Rha, Xyl, Glc, GalA and GlcA in a molar ration of 3.5:3.2:0.6:0.9:0.3:0.5:0.6:0.4. GP-C1 consisted of Gal, Ara, Man, Rha, Glc, and GlcA in the proportions of 2.1:1.0:0.3:0.5:0.4:0.9. Among them, GP-B1 treatment had a significant inhibitory effect on the growth of melanoma B16 in vivo and in vitro. Meanwhile GP-B1 could increase the relative spleen weight and stimulate the splenocyte proliferation alone or combined with ConA. Moreover, GP-B1 treatment induced an evident increase in the level of serum TNF-α, IFN-γ, and IL-12 and a reduction for IL-10 production. These results indicate that the antitumor effects of GP-B1 are associated with immunostimulation.
authors.affiliation: Department of Dermatology, The Second Hospital, Xi'an Jiaotong University, Xi'an 710004, China.
authors.forename: Xiao-Li
authors.initials: XL
authors.lastname: Li
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, The Second Hospital, Xi'an Jiaotong University, Xi'an 710004, China.
authors.forename: Zheng-Hui
authors.initials: ZH
authors.lastname: Wang
authors.affiliation: Department of Biological Science and Bioengineering, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China.
authors.forename: Yong-Xi
authors.initials: YX
authors.lastname: Zhao
authors.affiliation: Department of Dermatology, Tianjin Medical University General Hospital, Tianjin 300052, China.
authors.forename: Su-Ju
authors.initials: SJ
authors.lastname: Luo
authors.affiliation: Department of Dermatology, The Second Hospital, Xi'an Jiaotong University, Xi'an 710004, China.
authors.forename: Ding-Wei
authors.initials: DW
authors.lastname: Zhang
authors.affiliation: Department of Dermatology, The Second Hospital, Xi'an Jiaotong University, Xi'an 710004, China.
authors.forename: Sheng-Xiang
authors.initials: SX
authors.lastname: Xiao
authors.affiliation: Department of Dermatology, The Second Hospital, Xi'an Jiaotong University, Xi'an 710004, China.
authors.forename: Zhen-Hui
authors.initials: ZH
authors.lastname: Peng
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D016207:Cytokines / Q000378:metabolism; D029774:Gynostemma / Q000737:chemistry*; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D011134:Polysaccharides / Q000737:chemistry*
chemical_list: D016207:Cytokines; D010936:Plant Extracts; D011134:Polysaccharides