title: Optimization of dispersive liquid-liquid microextraction for preconcentration and spectrophotometric determination of phenols in Chabahar Bay seawater after derivatization with 4-aminoantipyrine.
abstract: We have optimized dispersive liquid-liquid microextraction to preconcentrate trace phenolic compounds after derivatization with 4-aminoantipyrine in artificial sea water for spectrophotometric determination. Factors such as reaction time (7.5 min), pH (9.5), solvent (chloroform), dispersing solvent (ethanol), and volume ratio of dispersing to organic phase (11:1) were optimized. Under optimum conditions, the limit of detection was 0.18 μg/L and the linearity range 1-900 μg/L. The relative standard deviation and enrichment factor were 6% (n=7) and 920, respectively. The results demonstrate the efficiency of coupling the 5530 APHA standard for derivation and dispersive liquid-liquid microextraction of phenolic compounds from seawater samples. Using this method, total phenol content in seawater from several locations in Chabahar Bay (southeast Iran) was estimated at 27.8-74.8 μg/L.
authors.affiliation: Department of Marine Chemistry, Faculty of Marine Sciences, Chabahar Maritime University, Chabahar, Iran.
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Nassiri
authors.affiliation: Department of Marine Chemistry, Faculty of Marine Sciences, Chabahar Maritime University, Chabahar, Iran. Electronic address: idm.m.zahedi@gmail.com.
authors.forename: Mir Mahdi
authors.initials: MM
authors.lastname: Zahedi
authors.affiliation: Faculty of Material and Manufacturing Technologies, Malek Ashtar University of Technology, Tehran, Iran. Electronic address: pourmortazavi@yahoo.com.
authors.forename: Seied Mahdi
authors.initials: SM
authors.lastname: Pourmortazavi
authors.affiliation: Department of Marine Chemistry, Faculty of Marine Sciences, Chabahar Maritime University, Chabahar, Iran.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Yousefzade
mesh_terms: D000675:Ampyrone / Q000032:analysis* / Q000737:chemistry*; D061006:Bays; D002725:Chloroform / Q000737:chemistry; D004396:Coloring Agents / Q000032:analysis; D005843:Geography; D006863:Hydrogen-Ion Concentration; D007492:Iran; D057230:Limit of Detection; D059627:Liquid Phase Microextraction; D019800:Phenol / Q000737:chemistry; D010636:Phenols / Q000032:analysis* / Q000737:chemistry; D012623:Seawater; D012997:Solvents / Q000737:chemistry; D013053:Spectrophotometry; D062065:Wastewater / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D000675:Ampyrone; D002725:Chloroform; D004396:Coloring Agents; D019800:Phenol; D010636:Phenols; D012997:Solvents; D062065:Wastewater; D014874:Water Pollutants, Chemical
keywords: 5530 APHA standard method; Chabahar Bay; Dispersive liquid–liquid microextraction (DLLME); Phenol determination; Seawater; Spectrophotometry