title: [Temporal regulation of transcription factor Mef2c by histone acetylases during cardiogenesis].
abstract: OBJECTIVE
  To observe the temporal modification of transcription factor Mef2c by histone acetylases (HATs) P300, PCAF, and SRC1 during cardiogenesis and to provide a basis for investigating the pathogenesis of congenital heart disease.
  
  
  METHODS
  The normal heart tissues from embryonic mice (embryonic days 14.5 and 16.5) and neonatal mice (postnatal days 0.5 and 7) were collected. The binding of P300, PCAF, and SRC1 to Mef2c gene and level of histone H3 acetylation in the promoter region of Mef2c were evaluated by chromatin immunoprecipitation assays. Meanwhile, real-time PCR was used to measure the mRNA expression of Mef2c.
  
  
  RESULTS
  P300, PCAF, SRC1 were involved in histone acetylation in the promoter region of Mef2c during cardiogenesis in mice, and binding of P300, PCAF, and SRC1 to the promoter of Mef2c varied significantly in different stages of cardiogenesis (P<0.01). The level of histone H3 acetylation and mRNA expression of Mef2c in the promoter region of Mef2c also varied significantly in different stages of cardiac development (P<0.01). The levels of acetylated H3, Mef2c mRNA, and HATs (P300, PCAF, SRC1) changed over time. They were highest on embryonic day 14.5 (P<0.01), decreased gradually with cardiac development, and were maintained at low levels after birth.
  
  
  CONCLUSIONS
  The mRNA expression of Mef2c varies during cardiogenesis in mice, which indicates that Mef2c plays an important role in the process of cardiac development. Meanwhile, histone acetylation in the promoter region of Mef2c is regulated temporally by HATs P300, PCAF, and SRC1.
authors.affiliation: Department of Cardiology, Children's Hospital of Chongqing Medical University, Chongqing 400014, China. jietian@cqmu.edu.cn.
authors.forename: Chang
authors.initials: C
authors.lastname: Peng
authors.forename: Wei-Hua
authors.initials: WH
authors.lastname: Zhang
authors.forename: Bo
authors.initials: B
authors.lastname: Pan
authors.forename: Wen-Qun
authors.initials: WQ
authors.lastname: Gao
authors.forename: Jie
authors.initials: J
authors.lastname: Tian
mesh_terms: D000818:Animals; D005260:Female; D018507:Gene Expression Regulation, Developmental; D006321:Heart / Q000196:embryology*; D051548:Histone Acetyltransferases / Q000502:physiology*; D064326:MEF2 Transcription Factors / Q000235:genetics / Q000502:physiology; D008297:Male; D051379:Mice; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000032:analysis
chemical_list: D064326:MEF2 Transcription Factors; C578041:Mef2c protein, mouse; D012333:RNA, Messenger; D051548:Histone Acetyltransferases