title: Nitrogen supply affects anthocyanin biosynthetic and regulatory genes in grapevine cv. Cabernet-Sauvignon berries.
abstract: Accumulation of anthocyanins in grape berries is influenced by environmental factors (such as temperature and light) and supply of nutrients, i.e., fluxes of carbon and nitrogen feeding the berry cells. It is established that low nitrogen supply stimulates anthocyanin production in berry skin cells of red varieties. The present works aims to gain a better understanding of the molecular mechanisms involved in the response of anthocyanin accumulation to nitrogen supply in berries from field grown-plants. To this end, we developed an integrated approach combining monitoring of plant nitrogen status, metabolite measurements and transcript analysis. Grapevines (cv. Cabernet-Sauvignon) were cultivated in a vineyard with three nitrogen fertilization levels (0, 60 and 120 kg ha(-1) of nitrogen applied on the soil). Anthocyanin profiles were analyzed and compared with gene expression levels. Low nitrogen supply caused a significant increase in anthocyanin levels at two ripening stages (26 days post-véraison and maturity). Delphinidin and petunidin derivatives were the most affected compounds. Transcript levels of both structural and regulatory genes involved in anthocyanin synthesis confirmed the stimulation of the phenylpropanoid pathway. Genes encoding phenylalanine ammonia-lyase (PAL), chalcone synthase (CHS), flavonoid-3',5'-hydroxylase (F3'5'H), dihydroflavonol-4-reductase (DFR), leucoanthocyanidin dioxygenase (LDOX) exhibited higher transcript levels in berries from plant cultivated without nitrogen compared to the ones cultivated with 120 kg ha(-1) nitrogen fertilization. The results indicate that nitrogen controls a coordinated regulation of both positive (MYB transcription factors) and negative (LBD proteins) regulators of the flavonoid pathway in grapevine.
authors.affiliation: Univ. Bordeaux, ISVV, EGFV, UMR 1287, F-33140 Villenave d'Ornon, France. Electronic address: eric.soubeyrand@bordeaux.inra.fr.
authors.forename: Eric
authors.initials: E
authors.lastname: Soubeyrand
authors.affiliation: INRA, ISVV, EGFV, UMR 1287, F-33140 Villenave d'Ornon, France. Electronic address: cyril.basteau@gmail.com.
authors.forename: Cyril
authors.initials: C
authors.lastname: Basteau
authors.affiliation: INRA, ISVV, EGFV, UMR 1287, F-33140 Villenave d'Ornon, France. Electronic address: hilbert@bordeaux.inra.fr.
authors.forename: Ghislaine
authors.initials: G
authors.lastname: Hilbert
authors.affiliation: Univ. Bordeaux, ISVV, EGFV, UMR 1287, F-33140 Villenave d'Ornon, France; Bordeaux Sciences Agro, ISVV, EGFV, UMR 1287, F-33140 Villenave d'Ornon, France. Electronic address: vanleeuwen@agro-bordeaux.fr.
authors.forename: Cornelis
authors.initials: C
authors.lastname: van Leeuwen
authors.affiliation: Univ. Bordeaux, ISVV, EGFV, UMR 1287, F-33140 Villenave d'Ornon, France. Electronic address: serge.delrot@bordeaux.inra.fr.
authors.forename: Serge
authors.initials: S
authors.lastname: Delrot
authors.affiliation: Univ. Bordeaux, ISVV, EGFV, UMR 1287, F-33140 Villenave d'Ornon, France. Electronic address: eric.gomes@bordeaux.inra.fr.
authors.forename: Eric
authors.initials: E
authors.lastname: Gomès
mesh_terms: D000429:Alcohol Oxidoreductases / Q000235:genetics; D000872:Anthocyanins / Q000378:metabolism*; D005638:Fruit / Q000187:drug effects / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000235:genetics; D009584:Nitrogen / Q000494:pharmacology*; D010650:Phenylalanine Ammonia-Lyase / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics; D027843:Vitis / Q000187:drug effects* / Q000378:metabolism*
chemical_list: D000872:Anthocyanins; D010940:Plant Proteins; D000429:Alcohol Oxidoreductases; C056570:dihydroflavanol 4-reductase; D010650:Phenylalanine Ammonia-Lyase; C017185:delphinidin; D009584:Nitrogen; C473206:petunidin
keywords: Flavonoid pathway; Grape berry; Lateral Organ Boundary Domain proteins; MYB transcription factor; Nitrogen