title: Two-dimensional correlation infrared spectroscopy applied to analyzing and identifying the extracts of Baeckea frutescens medicinal materials.
abstract: Baeckea frutescens or locally known as Cucur atap is used as antibacterial, antidysentery, antipyretic and diuretic agent. In Malaysia and Indonesia, they are used as an ingredient of the traditional medicine given to mothers during confinement. A three-steps infra-red (IR) macro-fingerprinting method combining conventional IR spectra, and the secondary derivative spectra with two dimensional infrared correlation spectroscopy (2D-IR) have been proved to be effective methods to examine a complicated mixture such as herbal medicines. This study investigated the feasibility of employing multi-steps IR spectroscopy in order to study the main constituents of B. frutescens and its different extracts (extracted by chloroform, ethyl acetate, methanol and aqueous in turn). The findings indicated that FT-IR and 2D-IR can provide many holistic variation rules of chemical constituents. The structural information of the samples indicated that B. frutescens and its extracts contain a large amount of flavonoids, since some characteristic absorption peaks of flavonoids, such as ∼1600cm(-1), ∼1500cm(-1), ∼1450cm(-1), and ∼1270cm(-1) can be observed. The macroscopical fingerprint characters of FT-IR and 2D-IR spectra can not only provide the information of main chemical constituents in medicinal materials and their different extracts, but also compare the components differences among the similar samples. In conclusion, the multi-steps IR macro-fingerprint method is rapid, effective, visual and accurate for pharmaceutical research.
authors.affiliation: Natural Products Division, Forest Research Institute Malaysia, 52109 Kepong, Selangor, Malaysia. Electronic address: adiana@frim.gov.my.
authors.forename: Adiana Mohamed
authors.initials: AM
authors.lastname: Adib
authors.affiliation: Natural Products Division, Forest Research Institute Malaysia, 52109 Kepong, Selangor, Malaysia.
authors.forename: Fadzureena
authors.initials: F
authors.lastname: Jamaludin
authors.affiliation: Natural Products Division, Forest Research Institute Malaysia, 52109 Kepong, Selangor, Malaysia.
authors.forename: Ling Sui
authors.initials: LS
authors.lastname: Kiong
authors.affiliation: Natural Products Division, Forest Research Institute Malaysia, 52109 Kepong, Selangor, Malaysia.
authors.forename: Nuziah
authors.initials: N
authors.lastname: Hashim
authors.affiliation: Natural Products Division, Forest Research Institute Malaysia, 52109 Kepong, Selangor, Malaysia.
authors.forename: Zunoliza
authors.initials: Z
authors.lastname: Abdullah
mesh_terms: D005240:Feasibility Studies; D005419:Flavonoids / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D007214:Indonesia; D008296:Malaysia; D008518:Medicine, East Asian Traditional; D027822:Myrtaceae / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D013055:Spectrophotometry, Infrared / Q000379:methods*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
chemical_list: D005419:Flavonoids; D010936:Plant Extracts; D012997:Solvents
keywords: Baeckea frutescens; FT-IR; Second derivative IR spectra; Two-dimensional correlation infrared spectroscopy