title: Decreasing quality of the new generations of anti-Müllerian hormone assays.
abstract: Anti-Müllerian hormone (AMH) measurements are widely used to optimize the stimulation protocols. First generation AMH kits correlated well with ovarian reserve and response to stimulation. In the present study we aimed to asses if the new generation kits share the same accurate correlations. Retrospective data were collected from 8323 blood samples. For comparison we used Immunotech I generation kit (ImI 4035 samples), Beckman Coulter II generation kit RUO (BCII RUO 3449, samples) and Beckman Coulter II generation kit with IVD certificate (BCII IVD 839 samples). We compared average AMH concentrations measured with different kits, as well as correlation between kits. We also compared average AMH concentrations in sera collected on different cycle days and samples of different quality of preservation. AMH serum concentrations differed for each kit, ranging 4.4 ± 4.12 (mean ± SD) for the ImI, 2.68 ± 3.15 for the BCII RUO, and 1.64 ± 2.85 for BCII IVD. The mean differences from an adjusted regression model were -48.7%, -40%, and -69.2%, respectively. In conclusion, the changes of the BC AMH kits are unpredictable; however, the improvement of them is still possible. It would be very dangerous to use elaborated stimulation protocol (based on the Ist generation AMH results) with the results from the IInd generation assays.
authors.affiliation: INVICTA Fertility and Reproductive Centre, 80-850 Gdansk, Poland ; Department of Nursing, Medical University, 80-952 Gdansk, Poland ; Department of Obstetrics and Gynecology, Faculty of Medical Sciences, Varmia and Masuria University, 10-561 Olsztyn, Poland ; INVICTA Fertility and Reproductive Centre, 00-019 Warsaw, Poland ; Department of Photophysics, IFFM Polish Academy of Sciences, 80-952 Gdansk, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Lukaszuk
authors.affiliation: INVICTA Fertility and Reproductive Centre, 80-850 Gdansk, Poland.
authors.forename: Beata
authors.initials: B
authors.lastname: Ludwikowska
authors.affiliation: INVICTA Fertility and Reproductive Centre, 80-850 Gdansk, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Liss
authors.affiliation: INVICTA Fertility and Reproductive Centre, 00-019 Warsaw, Poland.
authors.forename: Michal
authors.initials: M
authors.lastname: Kunicki
authors.affiliation: Department of Photophysics, IFFM Polish Academy of Sciences, 80-952 Gdansk, Poland.
authors.forename: Miroslaw
authors.initials: M
authors.lastname: Sawczak
authors.affiliation: INVICTA Fertility and Reproductive Centre, 80-850 Gdansk, Poland.
authors.forename: Aron
authors.initials: A
authors.lastname: Lukaszuk
authors.affiliation: INVICTA Fertility and Reproductive Centre, 80-850 Gdansk, Poland.
authors.forename: Lukasz
authors.initials: L
authors.lastname: Plociennik
authors.affiliation: Department of Obstetrics and Gynecology, The Medical Center of Postgraduate Education, 00-416 Warsaw, Poland.
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Jakiel
authors.affiliation: Department of Obstetrics and Gynecology, Faculty of Medical Sciences, Varmia and Masuria University, 10-561 Olsztyn, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Wasniewski
authors.affiliation: Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-747 Olsztyn, Poland.
authors.forename: Izabela
authors.initials: I
authors.lastname: Woclawek-Potocka
authors.affiliation: INVICTA Fertility and Reproductive Centre, 80-850 Gdansk, Poland ; INVICTA Fertility and Reproductive Centre, 00-019 Warsaw, Poland.
authors.forename: Dorota
authors.initials: D
authors.lastname: Bialobrzeska
mesh_terms: D000293:Adolescent; D000328:Adult; D054304:Anti-Mullerian Hormone / Q000737:chemistry*; D001681:Biological Assay / Q000379:methods*; D002648:Child; D005260:Female; D006801:Humans; D008875:Middle Aged; D011933:Reagent Kits, Diagnostic; D012189:Retrospective Studies; D055815:Young Adult
chemical_list: D011933:Reagent Kits, Diagnostic; D054304:Anti-Mullerian Hormone
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