title: Down-regulation of miR-141 induced by helicobacter pylori promotes the invasion of gastric cancer by targeting STAT4.
abstract: BACKGROUND
  The association between Helicobacter pylori infection and gastric cancer has been identified recently. However, the molecular mechanism remained largely unknown.
  
  
  RESULTS
  We found that miR-141 was decreased in Helicobacter pylori positive specimens (n=75) compared with negative tissues (n=75). The knockdown of miR-141 enhanced the invasion ability of gastric cancer cells; meanwhile, over-expression of miR-141 could inhibit the abilities of gastric cancer cells in vitro. A luciferase assay revealed that miR-141 was directly bound to the 3'-untranslated regions (3'-UTR) of STAT4. STAT4 was found up-regulated at mRNA and protein levels, as shown by qRT-PCR and western blot. Over-expression of STAT4 was used to mimic miR-141 action in the invasion of gastric cancer.
  
  
  CONCLUSIONS
  MiR-141 may play a pivotal role in controlling gastric cancer invasion through regulating STAT4 and maybe a potential target to treat gastric cancer.
authors.affiliation: Department of Gastroenterology, First Affiliated Hospital of Nanjing Medical University, First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Zhou
authors.forename: Yang
authors.initials: Y
authors.lastname: Xia
authors.forename: Jing
authors.initials: J
authors.lastname: Su
authors.forename: Guoxin
authors.initials: G
authors.lastname: Zhang
mesh_terms: D020413:3' Untranslated Regions; D000328:Adult; D000906:Antibodies / Q000097:blood; D001483:Base Sequence; D045744:Cell Line, Tumor; D002465:Cell Movement; D015536:Down-Regulation; D005260:Female; D016480:Helicobacter pylori / Q000276:immunology / Q000502:physiology*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000378:metabolism*; D008875:Middle Aged; D012333:RNA, Messenger / Q000378:metabolism; D050797:STAT4 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology; D015854:Up-Regulation
chemical_list: D020413:3' Untranslated Regions; D000906:Antibodies; C533895:MIRN141 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D050797:STAT4 Transcription Factor