title: Change in blood gelsolin concentration in response to physical exercise.
abstract: Plasma gelsolin (pGSN) produced by muscle is an abundant protein of extracellular fluids capable of severing actin filaments and eliminating actin from the circulation. Additionally, pGSN modulates the cellular effects of some bioactive lipids. In this study we test the hypothesis that hormonal and metabolic adaptations to exercise are associated with changes in gelsolin concentration in blood. Plasma samples were collected from twenty healthy males recruited from untrained (UT, n=10) and endurance trained (ET, n=10) groups that performed 30-60 minutes of exercise on a cycloergometer at a workload corresponding to 70% of VO2max. Gelsolin concentration was determined by quantitative Western blot analysis with an anti-human gelsolin antibody. The gelsolin concentration in UT and ET subjects before starting exercise ranged from 104 to 330 and 163 to 337 µg · ml(-1) respectively. After 30 minutes of exercise we observed a significant decrease of plasma gelsolin in the UT group (p<0.05) while the gelsolin concentration in the ET group rose on average from 244 to 271 µg · ml(-1). However, this increase did not reach statistical significance. Endurance training might increase the ability of muscle tissue to express plasma gelsolin as part of an adaptive mechanism.
authors.affiliation: Institute for Medicine and Engineering, University of Pennsylvania, 1010 Vagelos Research Laboratories, 3340 Smith Walk, Philadelphia, PA, 19104 U.S.A.
authors.forename: C-C
authors.initials: CC
authors.lastname: Yu
authors.affiliation: Department of Physiology, Medical University of Białystok, 15-230 Białystok, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Zendzian-Piotrowska
authors.affiliation: Department of Biochemistry, Faculty of Physical Education and Sport, Józef Piłsudski University of Physical Education, Biała Podlaska, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Charmas
authors.affiliation: Department of Biochemistry, Faculty of Physical Education and Sport, Józef Piłsudski University of Physical Education, Biała Podlaska, Poland.
authors.forename: B
authors.initials: B
authors.lastname: Długołęcka
authors.affiliation: Department of Physiology, Medical University of Białystok, 15-230 Białystok, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Baranowski
authors.affiliation: Department of Physiology, Medical University of Białystok, 15-230 Białystok, Poland.
authors.forename: J
authors.initials: J
authors.lastname: Górski
authors.affiliation: Institute for Medicine and Engineering, University of Pennsylvania, 1010 Vagelos Research Laboratories, 3340 Smith Walk, Philadelphia, PA, 19104 U.S.A.
authors.forename: R
authors.initials: R
authors.lastname: Bucki
keywords: blood; extracellular gelsolin; physical exercise
references.citation: Am J Respir Crit Care Med. 2000 Jul;162(1):288-94
references.pmid: 10903256
references.citation: Biochemistry. 2005 Jul 19;44(28):9590-7
references.pmid: 16008344
references.citation: J Cell Biol. 1988 Feb;106(2):375-84
references.pmid: 2828382
references.citation: Am J Physiol Cell Physiol. 2010 Dec;299(6):C1516-23
references.pmid: 20810916
references.citation: J Immunol. 2008 Oct 1;181(7):4936-44
references.pmid: 18802097
references.citation: EMBO J. 1998 Oct 15;17(20):5923-32
references.pmid: 9774337
references.citation: N Engl J Med. 1992 May 14;326(20):1335-41
references.pmid: 1314333
references.citation: Clin Exp Pharmacol Physiol. 1989 May;16(5):353-61
references.pmid: 2548778
references.citation: J Proteome Res. 2011 Oct 7;10(10):4769-88
references.pmid: 21823675
references.citation: Eur J Haematol. 2006 Jun;76(6):510-5
references.pmid: 16529602
references.citation: Cytokine. 2011 Jun;54(3):235-8
references.pmid: 21420877
references.citation: Am J Physiol Cell Physiol. 2007 Apr;292(4):C1323-30
references.pmid: 17135294
references.citation: Crit Care Med. 2007 Mar;35(3):849-55
references.pmid: 17205019
references.citation: J Neurochem. 1997 Sep;69(3):995-1005
references.pmid: 9282921
references.citation: Scand J Med Sci Sports. 2012 Apr;22(2):207-16
references.pmid: 20973830
references.citation: Gastroenterology. 1992 May;102(5):1686-92
references.pmid: 1314752
references.citation: Med Res Rev. 2012 Sep;32(5):999-1025
references.pmid: 22886630
references.citation: Neurodegener Dis. 2011;8(5):375-80
references.pmid: 21389683
references.citation: Sports Med. 2009;39(8):643-62
references.pmid: 19769414
references.citation: Stroke. 2011 Dec;42(12):3624-7
references.pmid: 21940962
references.citation: Curr Protein Pept Sci. 2008 Dec;9(6):541-51
references.pmid: 19075745
references.citation: J Biol Chem. 1984 Apr 25;259(8):5271-6
references.pmid: 6325429
references.citation: J Trauma Acute Care Surg. 2012 Feb;72(2):491-6
references.pmid: 22439219
references.citation: Antimicrob Agents Chemother. 2006 Sep;50(9):2932-40
references.pmid: 16940084
references.citation: Am J Respir Crit Care Med. 1999 Nov;160(5 Pt 1):1673-81
references.pmid: 10556139
references.citation: J Biol Chem. 1988 Jun 15;263(17):8239-43
references.pmid: 2836420
references.citation: Proc Natl Acad Sci U S A. 1990 Feb;87(4):1381-5
references.pmid: 2154744
references.citation: Blood. 1988 Jul;72(1):214-8
references.pmid: 2839253