title: A genetically encoded aldehyde for rapid protein labelling.
abstract: Using a mutant pyrrolysyl-tRNA synthetase-tRNA(Pyl)(CUA) pair, 3-formyl-phenylalanine is genetically incorporated into proteins at amber mutation sites in Escherichia coli. This non-canonical amino acid readily reacts with hydroxylamine dyes, leading to rapid and site-selective protein labelling.
authors.affiliation: Department of Chemistry, Texas A&M University, College Station, TX 77843, USA. wliu@chem.tamu.edu.
authors.forename: Alfred
authors.initials: A
authors.lastname: Tuley
authors.forename: Yan-Jiun
authors.initials: YJ
authors.lastname: Lee
authors.forename: Bo
authors.initials: B
authors.lastname: Wu
authors.forename: Zhiyong U
authors.initials: ZU
authors.lastname: Wang
authors.forename: Wenshe R
authors.initials: WR
authors.lastname: Liu
mesh_terms: D000447:Aldehydes / Q000737:chemistry*; D049452:Green Fluorescent Proteins / Q000737:chemistry* / Q000235:genetics*; D019811:Hydroxylamine / Q000032:analysis; D012343:RNA, Transfer / Q000032:analysis / Q000235:genetics; D013194:Staining and Labeling / Q000379:methods*; D013997:Time Factors
chemical_list: D000447:Aldehydes; D049452:Green Fluorescent Proteins; D019811:Hydroxylamine; D012343:RNA, Transfer
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