title: Full-Genome Sequencing of Four Bluetongue Virus Serotype 11 Viruses.
abstract: Recently, a contamination incident was described in which the challenge inoculum used in a bluetongue virus serotype 8 (BTV-8) vaccination trial was contaminated with a BTV-11 virus that was closely related to the Belgian BTV-11 virus from 2008. This study reports the first complete genome sequences of four BTV-11 viruses: the BTV-11 contaminant, BTV-11 reference strain, BTV-11 vaccine strain and a recently isolated BTV-11 field strain from Martinique. Full-genome analysis showed that these viruses belong to serotype 11/nucleotype A and cluster together with other western topotype bluetongue viruses. Detailed comparisons of the genomes further indicated that the contaminant was derived from the BTV-11 reference strain, as they were distinguished by a single synonymous nucleotide substitution. The previously reported partial sequence of genome segment 2 of the Belgian BTV-11 was found to be identical to that of the BTV-11 vaccine strain, indicating that it most likely was the BTV-11 vaccine strain. These findings also suggest that the BTV-11 contaminant and the Belgian BTV-11 are not the same viruses. Finally, comparison of the reference and vaccine strain did not allow determining the amino acid substitutions that contribute to the attenuated phenotype.
authors.affiliation: Operational Directorate of Viral Diseases, Molecular Platform, Veterinary and Agrochemical Research Centre, Ukkel, Belgium.
authors.forename: F
authors.initials: F
authors.lastname: Vandenbussche
authors.affiliation: ANSES, LSA (Animal Health Laboratory) UMR 1161 ANSES/INRA/ENVA, Maisons-Alfort, France.
authors.forename: C
authors.initials: C
authors.lastname: Sailleau
authors.affiliation: Operational Directorate of Viral Diseases, Molecular Platform, Veterinary and Agrochemical Research Centre, Ukkel, Belgium.
authors.forename: T
authors.initials: T
authors.lastname: Rosseel
authors.affiliation: ANSES, LSA (Animal Health Laboratory) UMR 1161 ANSES/INRA/ENVA, Maisons-Alfort, France.
authors.forename: A
authors.initials: A
authors.lastname: Desprat
authors.affiliation: ANSES, LSA (Animal Health Laboratory) UMR 1161 ANSES/INRA/ENVA, Maisons-Alfort, France.
authors.forename: C
authors.initials: C
authors.lastname: Viarouge
authors.affiliation: ANSES, LSA (Animal Health Laboratory) UMR 1161 ANSES/INRA/ENVA, Maisons-Alfort, France.
authors.forename: J
authors.initials: J
authors.lastname: Richardson
authors.affiliation: Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Eschbaumer
authors.affiliation: Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Hoffmann
authors.affiliation: Operational Directorate of Viral Diseases, Vesicular and Exotic Diseases, Veterinary and Agrochemical Research Centre, Ukkel, Belgium.
authors.forename: K
authors.initials: K
authors.lastname: De Clercq
authors.affiliation: ANSES, LSA (Animal Health Laboratory) UMR 1161 ANSES/INRA/ENVA, Maisons-Alfort, France.
authors.forename: E
authors.initials: E
authors.lastname: Bréard
authors.affiliation: ANSES, LSA (Animal Health Laboratory) UMR 1161 ANSES/INRA/ENVA, Maisons-Alfort, France.
authors.forename: S
authors.initials: S
authors.lastname: Zientara
mesh_terms: D000818:Animals; D001483:Base Sequence; D001819:Bluetongue / Q000517:prevention & control*; D001820:Bluetongue virus / Q000145:classification / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D005060:Europe; D016679:Genome, Viral / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D065288:Serogroup; D012756:Sheep; D014611:Vaccination / Q000662:veterinary; D014765:Viral Vaccines / Q000008:administration & dosage
chemical_list: D014765:Viral Vaccines
keywords: BTV-11; bluetongue virus; contamination; full-genome sequencing