title: Sequential axon-derived signals couple target survival and layer specificity in the Drosophila visual system.
abstract: Neural circuit formation relies on interactions between axons and cells within the target field. While it is well established that target-derived signals act on axons to regulate circuit assembly, the extent to which axon-derived signals control circuit formation is not known. In the Drosophila visual system, anterograde signals numerically match R1-R6 photoreceptors with their targets by controlling target proliferation and neuronal differentiation. Here we demonstrate that additional axon-derived signals selectively couple target survival with layer specificity. We show that Jelly belly (Jeb) produced by R1-R6 axons interacts with its receptor, anaplastic lymphoma kinase (Alk), on budding dendrites to control survival of L3 neurons, one of three postsynaptic targets. L3 axons then produce Netrin, which regulates the layer-specific targeting of another neuron within the same circuit. We propose that a cascade of axon-derived signals, regulating diverse cellular processes, provides a strategy for coordinating circuit assembly across different regions of the nervous system.
authors.affiliation: Department of Biological Chemistry, The Howard Hughes Medical Institute, The David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA.
authors.forename: Matthew Y
authors.initials: MY
authors.lastname: Pecot
authors.affiliation: Department of Biological Chemistry, The Howard Hughes Medical Institute, The David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA.
authors.forename: Yi
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Biological Chemistry, The Howard Hughes Medical Institute, The David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA.
authors.forename: Orkun
authors.initials: O
authors.lastname: Akin
authors.affiliation: Department of Biology, New York University, New York, NY 10003, USA.
authors.forename: Zhenqing
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Biological Chemistry, The Howard Hughes Medical Institute, The David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA.
authors.forename: C Y Kimberly
authors.initials: CY
authors.lastname: Tsui
authors.affiliation: Department of Biological Chemistry, The Howard Hughes Medical Institute, The David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA. Electronic address: LZipursky@mednet.ucla.edu.
authors.forename: S Lawrence
authors.initials: SL
authors.lastname: Zipursky
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism; D000077548:Anaplastic Lymphoma Kinase; D000818:Animals; D030801:Animals, Genetically Modified; D001369:Axons / Q000502:physiology*; D016923:Cell Death / Q000235:genetics; D004330:Drosophila; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D020439:Growth Cones / Q000378:metabolism; D034882:Lamins / Q000378:metabolism; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D009415:Nerve Net / Q000378:metabolism*; D009474:Neurons / Q000145:classification / Q000502:physiology*; D017956:Photoreceptor Cells, Invertebrate / Q000378:metabolism; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology*; D014795:Visual Pathways / Q000166:cytology* / Q000502:physiology*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D029721:Drosophila Proteins; D034882:Lamins; D008164:Luminescent Proteins; C413663:fluorescent protein 583; C440828:jeb protein, Drosophila; D049452:Green Fluorescent Proteins; D000077548:Anaplastic Lymphoma Kinase; D020794:Receptor Protein-Tyrosine Kinases
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