title: Protective effects of a wheat germ peptide (RVF) against H2O 2-induced oxidative stress in human neuroblastoma cells.
abstract: RVF (Arg-Val-Phe), a peptide derived from wheat germ, shows antioxidant properties. Here, the neuroprotective efficacies of RVF were investigated in human neuroblastoma cells (SH-SY5Y) that were pretreated with RVF (150-250 μM, 4 h) and exposed to H2O2 (200 μM). RVF increased viable cell numbers by 37 % and reduced the release of lactate dehydrogenase. Pretreatment with RVF also inhibited H2O2-induced accumulation of reactive oxygen species and maintained the mitochondrial transmembrane potential as well as preventing intracellular Ca(2+) dysregulation during H2O2 exposure. Furthermore, pretreatment with RVF increased the Bcl-2/Bax ratio and blocked cleavage poly(ADP-ribose) polymerase by inhibiting caspase-3 activation, thus decreasing apoptosis.
authors.affiliation: School of Chemistry and Biology Engineering, Changsha University of Science & Technology, Changsha, 410114, China, chengyh6488@gmail.com.
authors.forename: Yunhui
authors.initials: Y
authors.lastname: Cheng
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Sun
authors.forename: Jiqing
authors.initials: J
authors.lastname: Tang
authors.forename: Zhaoliang
authors.initials: Z
authors.lastname: Lv
authors.forename: Zhou
authors.initials: Z
authors.lastname: Xu
authors.forename: Huixin
authors.initials: H
authors.lastname: Yu
mesh_terms: D000975:Antioxidants / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D002118:Calcium / Q000378:metabolism; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D019610:Cytoprotection / Q000187:drug effects; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity*; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D009447:Neuroblastoma / Q000201:enzymology / Q000473:pathology*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D009842:Oligopeptides / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism
chemical_list: D000975:Antioxidants; D018696:Neuroprotective Agents; D009842:Oligopeptides; C000595224:RVF peptide; D006861:Hydrogen Peroxide; D011065:Poly(ADP-ribose) Polymerases; D053148:Caspase 3; D002118:Calcium