title: Structural investigation of an extracellular polysaccharide produced by the cariogenic bacterium Streptococcus mutans strain UA159.
abstract: The structure of an extracellular polysaccharide EPS159 produced from sucrose by Streptococcus mutans UA159 was investigated through the main oligosaccharides obtained from partial acid hydrolysis, monosaccharide/methylation analysis, and 1D/2D (1)H NMR spectroscopy. The results showed that EPS159 contained terminal, 3-substituted, 6-substituted, and 3,6-disubstituted α-D-glucopyranose residues in a molar percentage of 14, 18, 54, and 14%. The backbone of EPS159 was composed of →6)Glcp(1→ residues, and about 20% of the →6)Glcp(1→ residues was substituted at 3-OH by →3)Glcp(1→ and/or Glcp(1→ residues to form side chains. A composite model of EPS159, that includes all identified structural features, was formulated: [Formula, see text:].
authors.affiliation: School of Food Science, Henan Institute of Science and Technology, Hualan Road, 453003 Xinxiang, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Li
authors.affiliation: Department of Microbiology, University of Groningen, Nijenborgh 7, 8747 AG Groningen, The Netherlands.
authors.forename: Justyna M
authors.initials: JM
authors.lastname: Dobruchowska
authors.affiliation: Department of Preventive Dentistry, Academic Centre for Dentistry Amsterdam, University of Amsterdam and VU University Amsterdam, Gustav Mahlerlaan 3004, 1081 LA Amsterdam, The Netherlands.
authors.forename: Michel A
authors.initials: MA
authors.lastname: Hoogenkamp
authors.affiliation: Department of Microbiology, University of Groningen, Nijenborgh 7, 8747 AG Groningen, The Netherlands.
authors.forename: Gerrit J
authors.initials: GJ
authors.lastname: Gerwig
mesh_terms: D002326:Cariogenic Agents / Q000737:chemistry* / Q000378:metabolism*; D015394:Molecular Structure; D011134:Polysaccharides / Q000096:biosynthesis* / Q000737:chemistry*; D013295:Streptococcus mutans / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D002326:Cariogenic Agents; D011134:Polysaccharides