title: [Study on pharmacological ingredients of wuzhuyu tang treating migraine by correlating absorption ingredients in everted intestinal sac and pharmacodynamics].
abstract: Wuzhuyu Tang is a classical formula for treating migraine, but its' pharmacological ingredients is unclear yet. Present study employed the everted intestinal sac model to collect the absorption samples of 10 kinds of Wuzhuyu decoction, and then analyzed the contents of 9 ingredients in Wuzhuyu Tang and absorption samples quantitatively or semi-quantitatively by HPLC-DAD method. Reserpine was used to establish the mice model of migraine, and then the contents and activities of 5-hydroxytryptamine, noradrenaline, dopamine, nitric oxide and nitricoxide synthase in brain tissues and serums were determined respectively after oral administration of Wuzhuyu Tang. Using the partial least squares regression method to correlate the total absorption quantity of 9 ingredients and pharmacodynamics. The result shows that limocitrin-3-O-beta-D-glucoside, ginsenoside Rg1 and Rb1, rutaevine, limonin, evodiamine and rutaecarpine are the main ingredients influenced the effects in absorption samples in everted intestinal sacs, especially ginsenoside Rg1, rutaevine, evodiamine and rutaecarpine among them have obvious improving effects to most pharmacodynamics index, might be the pharmacological ingredients influenced the therapeutical effects of Wuzhuyu Tang treating migraine.
authors.forename: Xue-Qiang
authors.initials: XQ
authors.lastname: Pan
authors.forename: Yan-Chuan
authors.initials: YC
authors.lastname: Wu
authors.forename: Mu-Xin
authors.initials: MX
authors.lastname: Gong
authors.forename: Yong-Song
authors.initials: YS
authors.lastname: Xu
authors.forename: Zhi-Min
authors.initials: ZM
authors.lastname: Wang
authors.forename: Qi-Wei
authors.initials: QW
authors.lastname: Zhang
authors.forename: Ya-Wen
authors.initials: YW
authors.lastname: Shang
authors.forename: Xu-Ran
authors.initials: XR
authors.lastname: Lu
authors.forename: Ya-Fang
authors.initials: YF
authors.lastname: Song
mesh_terms: D000818:Animals; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D007408:Intestinal Absorption / Q000187:drug effects*; D007422:Intestines / Q000187:drug effects*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008881:Migraine Disorders / Q000188:drug therapy*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D004365:Drugs, Chinese Herbal