title: Purification and characterization of antifungal phenazines from a fluorescent Pseudomonas strain FPO4 against medically important fungi.
abstract: The strain FPO4 was isolated from the rhizoplane of rice plant root and identified as a fluorescent Pseudomonas aeruginosa on the basis of 16S rDNA sequences and BLAST analysis. The extracellular metabolites produced by this strain were purified by silica gel column chromatography and isolated four pure compounds. Based on the spectral data the four compounds were identified as phenazin-1-ol, phenazine-1-carboxylic acid (PCA), 2-heptyl-3-hydroxyl-4(1H)-quinolone (PQS), and phenazine-1-carboxamide (PCN), respectively. Phenazin-1-ol and PCA were active against all the eight fungi tested. The highest activity of 4 μg/mL by PCA was recorded against Trichophyton rubrum, a human pathogen responsible for causing athlete's foot, jock itch, ringworm and fingernail fungus infections, followed by Candida albicans and Candida tropicalis. The activity of phenazin-1-ol, PCA against Candida spp. was found to be better than the standard antifungal agent amphotericin B. Furthermore, the present study reports the antimicrobial activity of the purified phenazines on major human pathogen, T. rubrum for the first time.
authors.affiliation: Agroprocessing and Natural Products Division, National Institute for Interdisciplinary Science and Technology (NIIST), Council of Scientific and Industrial Research (CSIR), 695019 Thiruvanathapuram, Kerala, India.
authors.forename: J N
authors.initials: JN
authors.lastname: Gorantla
authors.affiliation: Agroprocessing and Natural Products Division, National Institute for Interdisciplinary Science and Technology (NIIST), Council of Scientific and Industrial Research (CSIR), 695019 Thiruvanathapuram, Kerala, India.
authors.forename: S Nishanth
authors.initials: SN
authors.lastname: Kumar
authors.affiliation: Agroprocessing and Natural Products Division, National Institute for Interdisciplinary Science and Technology (NIIST), Council of Scientific and Industrial Research (CSIR), 695019 Thiruvanathapuram, Kerala, India.
authors.forename: G V
authors.initials: GV
authors.lastname: Nisha
authors.affiliation: Department of Post Graduate Studies and Research in Botany, Sanatana Dharma College, 688003 Alappuzha, Kerala, India.
authors.forename: A S
authors.initials: AS
authors.lastname: Sumandu
authors.affiliation: Department of Post Graduate Studies and Research in Botany, Sanatana Dharma College, 688003 Alappuzha, Kerala, India.
authors.forename: C
authors.initials: C
authors.lastname: Dileep
authors.affiliation: Agroprocessing and Natural Products Division, National Institute for Interdisciplinary Science and Technology (NIIST), Council of Scientific and Industrial Research (CSIR), 695019 Thiruvanathapuram, Kerala, India.
authors.forename: A
authors.initials: A
authors.lastname: Sudaresan
authors.affiliation: Agroprocessing and Natural Products Division, National Institute for Interdisciplinary Science and Technology (NIIST), Council of Scientific and Industrial Research (CSIR), 695019 Thiruvanathapuram, Kerala, India.
authors.forename: M M Sree
authors.initials: MM
authors.lastname: Kumar
authors.affiliation: Agroprocessing and Natural Products Division, National Institute for Interdisciplinary Science and Technology (NIIST), Council of Scientific and Industrial Research (CSIR), 695019 Thiruvanathapuram, Kerala, India.
authors.forename: R S
authors.initials: RS
authors.lastname: Lankalapalli
authors.affiliation: Agroprocessing and Natural Products Division, National Institute for Interdisciplinary Science and Technology (NIIST), Council of Scientific and Industrial Research (CSIR), 695019 Thiruvanathapuram, Kerala, India. Electronic address: kumardileep@niist.res.in.
authors.forename: B S Dileep
authors.initials: BS
authors.lastname: Kumar
mesh_terms: D000935:Antifungal Agents / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D002175:Candida / Q000187:drug effects* / Q000502:physiology; D005453:Fluorescence; D006801:Humans; D008826:Microbial Sensitivity Tests; D010619:Phenazines / Q000302:isolation & purification / Q000494:pharmacology; D010802:Phylogeny; D011550:Pseudomonas aeruginosa / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D015363:Quinolones / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: C407944:2-heptyl-3-hydroxy-4-quinolone; D000935:Antifungal Agents; D010619:Phenazines; D015363:Quinolones; C420923:phenazine-1-carboxamide; C037165:1-phenazinecarboxylic acid
keywords: Activité antifongique; Antifungal activity; Phenazines; Phénazines; Pseudomonas