title: Monitoring the bulk milk antibody response to bovine viral diarrhea in dairy herds vaccinated with inactivated vaccines.
abstract: This study was designed to determine long-term responses in dairy herds after vaccination with 1 of 3 inactivated bovine viral diarrhea virus (BVDV) vaccines with regard to antibodies against p80 protein in bulk tank milk samples, as detected by ELISA. In the present study, 29 dairy herds were vaccinated with Bovilis BVD (MSD Animal Health, Milton Keynes, UK), 11 with Hiprabovis Balance (Laboratorios Hipra, Amer, Spain), and 9 with Pregsure BVD (Zoetis, Florham Park, NJ). In these herds, bulk tank milk samples were collected and examined at the time of the first vaccination and every 6 mo during a 3-yr period. Samples were analyzed with a commercial ELISA test for the p80 protein of BVDV. The results demonstrated that vaccination affected the level of antibodies against p80. Hence, vaccination status should be taken into consideration when interpreting bulk tank milk antibody tests.
authors.affiliation: ADSG Vacasan, Sobreira-Fornas, S/N, 27500 Chantada, Spain.
authors.forename: A M
authors.initials: AM
authors.lastname: Gonzalez
authors.affiliation: Animal Health and Production Laboratory of Galicia, Av. Madrid 77, 27002 Lugo, Spain.
authors.forename: I
authors.initials: I
authors.lastname: Arnaiz
authors.affiliation: Animal Health and Production Laboratory of Galicia, Av. Madrid 77, 27002 Lugo, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Eiras
authors.affiliation: Institute of Food Analysis and Research (Animal Health and Epidemiology Unit), Veterinary Faculty of Lugo, Santiago de Compostela University, Campus Universitario, 27002 Lugo, Spain.
authors.forename: F
authors.initials: F
authors.lastname: Camino
authors.affiliation: Institute of Food Analysis and Research (Animal Health and Epidemiology Unit), Veterinary Faculty of Lugo, Santiago de Compostela University, Campus Universitario, 27002 Lugo, Spain.
authors.forename: M L
authors.initials: ML
authors.lastname: Sanjuán
authors.affiliation: Institute of Food Analysis and Research (Animal Health and Epidemiology Unit), Veterinary Faculty of Lugo, Santiago de Compostela University, Campus Universitario, 27002 Lugo, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Yus
authors.affiliation: Institute of Food Analysis and Research (Animal Health and Epidemiology Unit), Veterinary Faculty of Lugo, Santiago de Compostela University, Campus Universitario, 27002 Lugo, Spain; Anatomy and Animal Production Department, Veterinary Faculty of Lugo, Santiago de Compostela University, Campus Universitario, 27002 Lugo, Spain. Electronic address: franciscojavier.dieguez@usc.es.
authors.forename: F J
authors.initials: FJ
authors.lastname: Diéguez
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000276:immunology*; D002417:Cattle; D003612:Dairying; D001908:Diarrhea Viruses, Bovine Viral / Q000502:physiology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D008892:Milk / Q000276:immunology* / Q000821:virology; D010447:Peptide Hydrolases / Q000276:immunology*; D020365:RNA Helicases / Q000276:immunology*; D013030:Spain; D014611:Vaccination / Q000662:veterinary; D015164:Vaccines, Inactivated / Q000008:administration & dosage / Q000276:immunology; D017361:Viral Nonstructural Proteins / Q000276:immunology*; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*; D012697:Serine Endopeptidases; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases
chemical_list: D000914:Antibodies, Viral; D010447:Peptide Hydrolases; D020365:RNA Helicases; D015164:Vaccines, Inactivated; D017361:Viral Nonstructural Proteins; D014765:Viral Vaccines; C086730:p80 protein, bovine viral diarrhea virus; D012697:Serine Endopeptidases; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases
keywords: ELISA; bovine viral diarrhea virus (BVDV); diagnosis; milk; vaccine