title: Proteome array identification of bioactive soluble proteins/peptides in Matrigel: relevance to stem cell responses.
abstract: Matrigel and similar commercial products are extracts of the Engelbreth-Holm-Swarm sarcoma that provide a basement-membrane-like attachment substrate or gel that is used to grow cells on or in, respectively. To ascertain further what proteins may be present in Matrigel, besides its major basement-membrane constituents, an analysis of the expressed liquid of gelled Matrigel was performed using proteome array technology. Among the growth factors/cytokines assayed, high positive detection was found for IGFBP1, IGFBP3, LIF, platelet factor 4, PlGF-2, and VEGF; moderate reactivity was found for cyr61, IGFBP2, IGFBP6, IL-1ra, and NOV; and low, but detectable, responses occurred for aFGF, IL-13, IL-23, M-CSF, and VEGF-B. Among the chemokines assayed, high positive detection was found for MIG and serpin E1; moderate reactivity was found for IP-10, MCP-1, and MCP-5, and low, but detectable, responses occurred for CXCL16, I-TAC, and MIP-1α. Among the other biologically active proteins assayed, high positive detection was found for adiponectin, C5a, endocan, lipocalin-2, sICAM-1, MMP-3, and TIMP-1; moderate reactivity was found for C-reactive protein, coagulation factor III, endoglin, endostatin/collagen XVIII, endothelin-1, ICAM-1, MMP-9, osteopontin, pentraxin-3, and RANTES; and low, but detectable, responses occurred for fetuin A, MMP-8, pentraxin-2, RBP4, resistin, and TIMP-4. The study found several growth factors, chemokines, and biologically active proteins not previously identified in Matrigel, and this may have significance to the interpretations of observed cellular responses when cells are grown on or in Matrigel.
authors.affiliation: Agricultural Research Service (ARS), Animal Biosciences and Biotechnology Laboratory, Beltsville Agricultural Research Center (BARC), U. S. Department of Agriculture (USDA), BARC-East, Bldg. 200, Rm. 13, 20705, Beltsville, MD, USA, neil.talbot@ars.usda.gov.
authors.forename: Neil C
authors.initials: NC
authors.lastname: Talbot
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Caperna
references.citation: Stem Cells. 2011 Aug;29(8):1196-205
references.pmid: 21681859
references.citation: Cancer Res. 1987 Jun 15;47(12):3239-45
references.pmid: 2438036
references.citation: Cell. 2012 Oct 26;151(3):547-58
references.pmid: 23101625
references.citation: PLoS One. 2013;8(2):e55530
references.pmid: 23424635
references.citation: Toxicol In Vitro. 2005 Apr;19(3):389-97
references.pmid: 15713546
references.citation: PLoS One. 2013 Aug 02;8(8):e67861
references.pmid: 23936296
references.citation: Hepat Med. 2010 Feb 1;2010(2):13-32
references.pmid: 21852899
references.citation: Int J Cardiol. 2010 May 14;141(1):49-60
references.pmid: 19138802
references.citation: Nature. 2002 Jun 27;417(6892):954-8
references.pmid: 12087404
references.citation: Arterioscler Thromb Vasc Biol. 2011 Mar;31(3):582-9
references.pmid: 21212399
references.citation: J Cell Physiol. 1988 Mar;134(3):309-23
references.pmid: 3350857
references.citation: Proc Natl Acad Sci U S A. 2007 Jun 19;104(25):10595-600
references.pmid: 17567755
references.citation: Neurochem Res. 2011 Mar;36(3):406-11
references.pmid: 21136151
references.citation: BMC Dev Biol. 2008 Sep 22;8:90
references.pmid: 18808690
references.citation: Nat Biotechnol. 2001 Oct;19(10):971-4
references.pmid: 11581665
references.citation: Proteomics. 2010 May;10(9):1886-90
references.pmid: 20162561
references.citation: Stem Cells Dev. 2004 Aug;13(4):325-36
references.pmid: 15345125
references.citation: Exp Cell Res. 1992 Sep;202(1):1-8
references.pmid: 1511725
references.citation: Mol Cell Proteomics. 2009 Jul;8(7):1648-57
references.pmid: 19351662
references.citation: In Vitro Cell Dev Biol Anim. 2010 Oct;46(9):746-57
references.pmid: 20607619
references.citation: In Vitro Cell Dev Biol Anim. 1995 Jun;31(6):404-11
references.pmid: 8589878
references.citation: J Mammary Gland Biol Neoplasia. 2012 Jun;17(2):103-10
references.pmid: 22573197
references.citation: Stem Cells. 2013 Jun;31(6):1097-106
references.pmid: 23404867
references.citation: Tumori. 2003 Jul-Aug;89(4):421-5
references.pmid: 14606648
references.citation: Dev Biol. 1990 Oct;141(2):344-52
references.pmid: 2120094
references.citation: Biochemistry. 1982 Nov 23;21(24):6188-93
references.pmid: 6217835
references.citation: In Vitro Cell Dev Biol Anim. 2012 Jan;48(1):1-11
references.pmid: 22179674
references.citation: Lab Invest. 2003 Dec;83(12):1811-20
references.pmid: 14691299
references.citation: Semin Cancer Biol. 2005 Oct;15(5):378-86
references.pmid: 15975825
references.citation: Blood. 2007 Dec 1;110(12):4111-9
references.pmid: 17761519
references.citation: J Neurosci Res. 2010 Feb 15;88(3):542-51
references.pmid: 19774667
references.citation: J Exp Med. 1977 Jan 1;145(1):204-20
references.pmid: 830788
references.citation: Stem Cells. 2005 Feb;23(2):288-96
references.pmid: 15671151
references.citation: Hum Gene Ther. 2013 Jul;24(7):655-69
references.pmid: 23659480
references.citation: PLoS One. 2011;6(9):e25663
references.pmid: 21980522
references.citation: J Microsc. 2013 Sep;251(3):212-23
references.pmid: 23432616
references.citation: Biochemistry. 1986 Jan 28;25(2):312-8
references.pmid: 2937447
references.citation: Tissue Eng Part A. 2012 Mar;18(5-6):609-20
references.pmid: 21981309
references.citation: PLoS One. 2013 Aug 07;8(8):e71104
references.pmid: 23951091
references.citation: Placenta. 2002 Sep-Oct;23(8-9):613-30
references.pmid: 12361681
references.citation: J Genet Genomics. 2010 Jul;37(7):451-60
references.pmid: 20659709
references.citation: Shock. 1995 Sep;4(3):155-60
references.pmid: 8574748
references.citation: Blood. 1998 Apr 1;91(7):2283-95
references.pmid: 9516126