title: Cytokines transcript levels in lung and lymphoid organs during genotype 1 Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) infection.
abstract: Porcine Reproductive and Respiratory Syndrome (PRRS) is one of the most economically important diseases of swine. PRRS virus (PRRSV) infection in the pig is characterized by a weak or absent host innate immune response. The underlying mechanisms of PRRSV pathogenesis are still unclear. The analysis of transcript levels represents an alternative to immunoassays for the detection of cytokines that sometimes are difficult to detect due to their low amounts. This study sets out to determine the differences in pathogenesis and the immune response between lung, tonsil, tracheobronchial lymph node (Tb-LN) and retropharyngeal LN (Rf-LN) of PRRSV 2982 strain infected pigs. PRRSV strain 2982 avoided the onset of an effective innate immune response, especially in PRRSV main target (lung) and reservoir (tonsil) organs. PRRSV lead to an impaired expression of IFN-α and TNF-α gene expression, which finally induced a weak and delayed adaptive immune response through an inefficient IL-12 and IFN-γ expression. Finally, PRRSV replication favored the expression of the anti-inflammatory IL-10 cytokine in infected pigs.
authors.affiliation: Department of Anatomy and Comparative Pathology, Faculty of Veterinary Medicine, Murcia University,"Mare Nostrum Excellence Campus-3738", 30100 Murcia, Spain. Electronic address: ogn14346@um.es.
authors.forename: Obdulio
authors.initials: O
authors.lastname: García-Nicolás
authors.affiliation: Spanish National Center of Biotechnology (CSIC), C/Darwin 3, Campus de Cantoblanco, 28049Madrid, Spain.
authors.forename: Juan José
authors.initials: JJ
authors.lastname: Quereda
authors.affiliation: CICAP - Agrifood Research Centre, 14400 Pozoblanco, Córdoba, Spain.
authors.forename: Jaime
authors.initials: J
authors.lastname: Gómez-Laguna
authors.affiliation: School of Veterinary Medicine. Faculty of Health and Medical Sciences. University of Surrey, Guildford GU2 7TE, UK.
authors.forename: Francisco Javier
authors.initials: FJ
authors.lastname: Salguero
authors.affiliation: Department of Anatomy and Comparative Pathology, Faculty of Veterinary Medicine, Cordoba University, Agrifood Campus of International Excellence - CeiA3, 14071 Cordoba, Spain.
authors.forename: Librado
authors.initials: L
authors.lastname: Carrasco
authors.affiliation: Department of Animal Production, Faculty of Veterinary Medicine, Murcia University,"Mare Nostrum Excellence Campus-3738", 30100 Murcia, Spain.
authors.forename: Guillermo
authors.initials: G
authors.lastname: Ramis
authors.affiliation: Department of Anatomy and Comparative Pathology, Faculty of Veterinary Medicine, Murcia University,"Mare Nostrum Excellence Campus-3738", 30100 Murcia, Spain.
authors.forename: Francisco José
authors.initials: FJ
authors.lastname: Pallarés
mesh_terms: D000818:Animals; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D008168:Lung / Q000378:metabolism* / Q000473:pathology; D008221:Lymphoid Tissue / Q000378:metabolism*; D019318:Porcine Reproductive and Respiratory Syndrome / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics*; D013047:Specific Pathogen-Free Organisms; D013552:Swine; D014766:Viremia; D014779:Virus Replication
chemical_list: D016207:Cytokines
keywords: Cytokines; Immune response; PRRSV; Transcript levels