title: Validation of DNA methylation profiling in formalin-fixed paraffin-embedded samples using the Infinium HumanMethylation450 Microarray.
abstract: A formalin-fixed paraffin-embedded (FFPE) sample usually yields highly degraded DNA, which limits the use of techniques requiring high-quality DNA, such as Infinium Methylation microarrays. To overcome this restriction, we have applied an FFPE restoration procedure consisting of DNA repair and ligation processes in a set of paired fresh-frozen (FF) and FFPE samples. We validated the FFPE results in comparison with matched FF samples, enabling us to use FFPE samples on the Infinium HumanMethylation450 Methylation array.
authors.affiliation: Cancer Epigenetics and Biology Program (PEBC); Bellvitge Biomedical Research Institute (IDIBELL); Barcelona, Catalonia, Spain.
authors.forename: Sebastián
authors.initials: S
authors.lastname: Moran
authors.affiliation: Cancer Epigenetics and Biology Program (PEBC); Bellvitge Biomedical Research Institute (IDIBELL); Barcelona, Catalonia, Spain.
authors.forename: Miguel
authors.initials: M
authors.lastname: Vizoso
authors.affiliation: Cancer Epigenetics and Biology Program (PEBC); Bellvitge Biomedical Research Institute (IDIBELL); Barcelona, Catalonia, Spain.
authors.forename: Anna
authors.initials: A
authors.lastname: Martinez-Cardús
authors.affiliation: Cancer Epigenetics and Biology Program (PEBC); Bellvitge Biomedical Research Institute (IDIBELL); Barcelona, Catalonia, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Gomez
authors.affiliation: Department of Pathology and Molecular Genetics, and Research Laboratory; Arnau de Vilanova Universitary Hospital; University of Lleida IRBLLEIDA; Lleida, Catalonia, Spain.
authors.forename: Xavier
authors.initials: X
authors.lastname: Matías-Guiu
authors.affiliation: Ferrer Advanced Biotherapeutics; Barcelona, Catalonia, Spain.
authors.forename: Sebastián M
authors.initials: SM
authors.lastname: Chiavenna
authors.affiliation: Ferrer Advanced Biotherapeutics; Barcelona, Catalonia, Spain.
authors.forename: Andrés G
authors.initials: AG
authors.lastname: Fernandez
authors.affiliation: Cancer Epigenetics and Biology Program (PEBC); Bellvitge Biomedical Research Institute (IDIBELL); Barcelona, Catalonia, Spain; Department of Physiological Sciences II; School of Medicine; University of Barcelona; Barcelona, Catalonia, Spain; Institució Catalana de Recerca i Estudis Avançats (ICREA); Barcelona, Catalonia, Spain.
authors.forename: Manel
authors.initials: M
authors.lastname: Esteller
mesh_terms: D019175:DNA Methylation; D004260:DNA Repair; D005404:Fixatives; D005557:Formaldehyde; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016612:Paraffin Embedding
chemical_list: D005404:Fixatives; D005557:Formaldehyde
keywords: 450K; DNA methylation; FFPE; epigenetics; infinium; methylation; microarray
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