title: In vivo construction of tissue-engineered cartilage using adipose-derived stem cells and bioreactor technology.
abstract: The present study aims to investigate the feasibility of tissue-engineered cartilage constructed in vivo and in vitro by dynamically culturing adipose-derived stem cells (ADSCs) with an articular cartilage acellular matrix in a bioreactor and subsequently implanting the cartilage in nude mice. ADSCs were proliferated, combined with three dimensional scaffolds (cell density: 5 × 10(7)/mL) and subsequently placed in a bioreactor and culture plate for 3 weeks. In the in vivo study, complexes cultured for 1 week under dynamic or static states were subcutaneously implanted into nude mice and collected after 3 weeks. Indicators such as gross morphology, histochemistry and immunohistochemistry were examined. In the in vitro study, histological observation showed that most scaffolds in the dynamic group were absorbed, and cell proliferation and matrix secretion were significant. Positive staining of safranin-O and alcian blue II collagen stain in the dynamic group was significantly stronger than that in the static culture group. In the in vivo study, cartilage-like tissues formed in the specimens of the two groups. Histological examination showed that cell distribution in the dynamic group was relatively more uniform than in the static group, and matrix secretion was relatively stronger. Bioreactor culturing can promote ADSC proliferation and cartilage differentiation and is thus a suitable method for constructing tissue-engineered cartilage in vivo.
authors.affiliation: Key Laboratory of PLA, Department of Orthopaedics, Chinese PLA General Hospital, No. 28 Fuxing Road, Haidian District, Beijing, 100853, China.
authors.forename: Hongjun
authors.initials: H
authors.lastname: Kang
authors.forename: Shibi
authors.initials: S
authors.lastname: Lu
authors.forename: Jiang
authors.initials: J
authors.lastname: Peng
authors.forename: Qiang
authors.initials: Q
authors.lastname: Yang
authors.forename: Shuyun
authors.initials: S
authors.lastname: Liu
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Jingxiang
authors.initials: J
authors.lastname: Huang
authors.forename: Xiang
authors.initials: X
authors.lastname: Sui
authors.forename: Bin
authors.initials: B
authors.lastname: Zhao
authors.forename: Aiyuan
authors.initials: A
authors.lastname: Wang
authors.forename: Wenjing
authors.initials: W
authors.lastname: Xu
authors.forename: Quanyi
authors.initials: Q
authors.lastname: Guo
authors.forename: Qing
authors.initials: Q
authors.lastname: Song
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D019149:Bioreactors; D002356:Cartilage / Q000166:cytology*; D005260:Female; D051379:Mice; D008819:Mice, Nude; D008855:Microscopy, Electron, Scanning; D013234:Stem Cells; D023822:Tissue Engineering; D054457:Tissue Scaffolds