title: Assessment of cell death studies by monitoring hydrogen peroxide in cell culture.
abstract: Hydrogen peroxide (H2O2) has been widely used to study the oxidative stress response. However, H2O2 is unstable and easily decomposes into H2O and O2. Consequently, a wide range of exposure times and treatment concentrations has been described in the literature. In the present study, we established a ferrous oxidation-xylenol orange (FOX) assay, which was originally described for food and body liquids, as a method for the precise quantification of H2O2 concentrations in cell culture media. We observed that the presence of FCS and high cell densities significantly accelerate the decomposition of H2O2, therefore acting as a protection against cell death by accidental necrosis.
authors.affiliation: Max Planck Research Unit for Enzymology of Protein Folding, Weinbergweg 22, 06120 Halle, Germany. Electronic address: irina-hirsch@gmx.de.
authors.forename: Irina
authors.initials: I
authors.lastname: Hirsch
authors.affiliation: Max Planck Research Unit for Enzymology of Protein Folding, Weinbergweg 22, 06120 Halle, Germany.
authors.forename: Erik
authors.initials: E
authors.lastname: Prell
authors.affiliation: Institut für Biochemie und Biotechnologie, Martin-Luther-Universität Halle-Wittenberg, Weinbergweg 22, 06120 Halle (Saale), Germany. Electronic address: matthias.weiwad@biochemtech.uni-halle.de.
authors.forename: Matthias
authors.initials: M
authors.lastname: Weiwad
mesh_terms: D016475:3T3 Cells; D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D016923:Cell Death / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003470:Culture Media / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000032:analysis* / Q000494:pharmacology*; D051379:Mice; D013997:Time Factors
chemical_list: D003470:Culture Media; D006861:Hydrogen Peroxide
keywords: Accidental/oncotic necrosis; Apoptosis; Cell death; Ferrous oxidation-xylenol orange (FOX) assay; Hydrogen peroxide (H(2)O(2)); Secondary/apoptotic necrosis