title: Detection of subtle neurological alterations by the Catwalk XT gait analysis system.
abstract: BACKGROUND
  A new version of the CatWalk XT system was evaluated as a tool for detecting very subtle alteration in gait based on higher speed sample rate; the system could also demonstrate minor changes in neurological function. In this study, we evaluated the neurological outcome of sciatic nerve injury intervened by local injection of hyaluronic acid. Using the CatWalk XT system, we looked for differences between treated and untreated groups and differences within the same group as a function of time so as to assess the power of the Catwalk XT system for detecting subtle neurological change.
  
  
  METHODS
  Peripheral nerve injury was induced in 36 Sprague-Dawley rats by crushing the left sciatic nerve using a vessel clamp. The animals were randomized into one of two groups: Group I: crush injury as the control; Group II: crush injury and local application with hyaluronic acid. These animals were subjected to neurobehavior assessment, histomorphology evaluation, and electrophysiology study periodically. These data were retrieved for statistical analysis.
  
  
  RESULTS
  The density of neurofilament and S-100 over the distal end of crushed nerve showed significant differences either in inter-group comparison at various time points or intra-group comparison from 7 to 28 days. Neuronal structure architecture, axon counts, intensity of myelination, electrophysiology, and collagen deposition demonstrate significant differences between the two groups. There was significant difference of SFI and angle of ankle in inter- group analysis from 7 to 28 days, but there were no significant differences in SFI and angle of ankle at time points of 7 and 14 days. In the Cat Walk XT analysis, the intensity, print area, stance duration, and swing duration all showed detectable differences at 7, 14, 21, and 28 days, whereas there were no significant difference at 7 and 14 days with CatWalk 7 testing. In addition, there were no significant differences of step sequence or regularity index between the two versions.
  
  
  CONCLUSIONS
  Hyaluronic acid augmented nerve regeneration as early as 7 days after crush injury. This subtle neurological alteration could be detected through the CatWalk XT gait analysis but not the SFI, angle of ankle, or CatWalk 7 methods.
authors.forename: Ying-Ju
authors.initials: YJ
authors.lastname: Chen
authors.forename: Fu-Chou
authors.initials: FC
authors.lastname: Cheng
authors.forename: Meei-Ling
authors.initials: ML
authors.lastname: Sheu
authors.forename: Hong-Lin
authors.initials: HL
authors.lastname: Su
authors.forename: Chun-Jung
authors.initials: CJ
authors.lastname: Chen
authors.forename: Jason
authors.initials: J
authors.lastname: Sheehan
authors.affiliation: Department of Neurosurgery, Taichung Veterans General Hospital, No, 160, Taichung Port Road, Sec, 3, Taichung 407, Taiwan. hcpan2003@yahoo.com.tw.
authors.forename: Hung-Chuan
authors.initials: HC
authors.lastname: Pan
mesh_terms: D000818:Animals; D004594:Electrophysiology; D006820:Hyaluronic Acid / Q000494:pharmacology*; D007150:Immunohistochemistry; D007794:Lameness, Animal / Q000175:diagnosis* / Q000209:etiology; D009409:Nerve Crush; D009416:Nerve Regeneration / Q000187:drug effects; D059348:Peripheral Nerve Injuries / Q000150:complications / Q000175:diagnosis* / Q000503:physiopathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function / Q000187:drug effects; D012584:Sciatic Nerve / Q000293:injuries
chemical_list: D006820:Hyaluronic Acid
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