title: Microtiter plate-sized standalone chip holder for microenvironmental physiological control in gas-impermeable microfluidic devices.
abstract: We present a microtiter plate-sized standalone chip holder for precise control of physiological conditions inside closed microfluidic cell culture systems, made from gas-impermeable materials. Specifically, we demonstrate the suitability of the holder to support cell growth in a glass chip, to allow time-lapse imaging of live cells and the creation of a hypoxic environment, all relevant for applications in regenerative medicine research.
authors.affiliation: Department of Tissue Regeneration, MIRA Institute for Biomedical Technology and Technical Medicine, Enschede, The Netherlands. bjornharink@gmail.com p.habibovic@utwente.nl.
authors.forename: Björn
authors.initials: B
authors.lastname: Harink
authors.forename: Séverine
authors.initials: S
authors.lastname: Le Gac
authors.forename: David
authors.initials: D
authors.lastname: Barata
authors.forename: Clemens
authors.initials: C
authors.lastname: van Blitterswijk
authors.forename: Pamela
authors.initials: P
authors.lastname: Habibovic
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods; D002460:Cell Line; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D051379:Mice; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods; D009757:Nylons / Q000737:chemistry; D044968:Regenerative Medicine
chemical_list: D004129:Dimethylpolysiloxanes; D009757:Nylons; C085707:poly(dimethylsiloxane)-polyamide copolymer