title: Transcriptomic profiling as a screening tool to detect trenbolone treatment in beef cattle.
abstract: The effects of steroid hormone implants containing trenbolone alone (Finaplix-H), combined with 17β-oestradiol (17β-E; Revalor-H), or with 17β-E and dexamethasone (Revalor-H plus dexamethasone per os) on the bovine muscle transcriptome were examined by DNA-microarray. Overall, large sets of genes were shown to be modulated by the different growth promoters (GPs) and the regulated pathways and biological processes were mostly shared among the treatment groups. Using the Prediction Analysis of Microarray program, GP-treated animals were accurately identified by a small number of predictive genes. A meta-analysis approach was also carried out for the Revalor group to potentially increase the robustness of class prediction analysis. After data pre-processing, a high level of accuracy (90%) was obtained in the classification of samples, using 105 predictive gene markers. Transcriptomics could thus help in the identification of indirect biomarkers for anabolic treatment in beef cattle to be applied for the screening of muscle samples collected after slaughtering.
authors.affiliation: Department of Comparative Biomedicine and Food Science, University of Padua, Viale dell'Università 16, 35020 Legnaro, Padova, Italy. Electronic address: sara.pegolo@unipd.it.
authors.forename: S
authors.initials: S
authors.lastname: Pegolo
authors.affiliation: Department of Animal Pathology, University of Turin, via L. da Vinci 44, 10095, Grugliasco, Italy.
authors.forename: F T
authors.initials: FT
authors.lastname: Cannizzo
authors.affiliation: Department of Animal Pathology, University of Turin, via L. da Vinci 44, 10095, Grugliasco, Italy.
authors.forename: B
authors.initials: B
authors.lastname: Biolatti
authors.affiliation: Department of Comparative Biomedicine and Food Science, University of Padua, Viale dell'Università 16, 35020 Legnaro, Padova, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Castagnaro
authors.affiliation: Department of Comparative Biomedicine and Food Science, University of Padua, Viale dell'Università 16, 35020 Legnaro, Padova, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Bargelloni
mesh_terms: D045930:Anabolic Agents / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002417:Cattle / Q000378:metabolism*; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics; D011897:Random Allocation; D059467:Transcriptome / Q000235:genetics; D014204:Trenbolone Acetate / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D045930:Anabolic Agents; D012333:RNA, Messenger; D014204:Trenbolone Acetate
keywords: Bovine; DNA-microarray; Hormone implants; Trenbolone