title: A novel approach to the site-selective dual labelling of a proteinviachemoselective cysteine modification.
abstract: Local protein microenvironment is used to control the outcome of reaction between cysteine residues and 2,5-dibromohexanediamide. The differential reactivity is exploited to introduce two orthogonal reactive handles onto the surface of a double cysteine mutant of superfolder green fluorescent protein in a regioselective manner. Subsequent elaboration with commonly used thiol and alkyne containing reagents affects site-selective protein dual labelling.
authors.affiliation: Department of Chemistry , University College London , 20 Gordon Street , London , WC1H OAJ , UK . Email: VPEnterprise@ucl.ac.uk ; ; Tel: +44 (0)20 3108 5071.
authors.forename: Ramiz I
authors.initials: RI
authors.lastname: Nathani
authors.affiliation: Department of Chemistry , University College London , 20 Gordon Street , London , WC1H OAJ , UK . Email: VPEnterprise@ucl.ac.uk ; ; Tel: +44 (0)20 3108 5071.
authors.forename: Paul
authors.initials: P
authors.lastname: Moody
authors.affiliation: Department of Chemistry , University College London , 20 Gordon Street , London , WC1H OAJ , UK . Email: VPEnterprise@ucl.ac.uk ; ; Tel: +44 (0)20 3108 5071.
authors.forename: Vijay
authors.initials: V
authors.lastname: Chudasama
authors.affiliation: Department of Chemistry , University College London , 20 Gordon Street , London , WC1H OAJ , UK . Email: VPEnterprise@ucl.ac.uk ; ; Tel: +44 (0)20 3108 5071.
authors.forename: Mark E B
authors.initials: ME
authors.lastname: Smith
authors.affiliation: Department of Chemistry , University College London , 20 Gordon Street , London , WC1H OAJ , UK . Email: VPEnterprise@ucl.ac.uk ; ; Tel: +44 (0)20 3108 5071.
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Fitzmaurice
authors.affiliation: Department of Chemistry , University College London , 20 Gordon Street , London , WC1H OAJ , UK . Email: VPEnterprise@ucl.ac.uk ; ; Tel: +44 (0)20 3108 5071.
authors.forename: Stephen
authors.initials: S
authors.lastname: Caddick
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