title: Enhancement of amygdalin activated with β-D-glucosidase on HepG2 cells proliferation and apoptosis.
abstract: The growth inhibition and induction of apoptosis brought by amygdalin and activated with β-D-glucosidase were tested for cytoactivity in HepG2 cells. The MTT viability assay showed that all samples had effects on HepG2 proliferation in dose and time response manners. IC50 of stand-alone amygdalin and activation with β-D-glucosidase on the proliferation of HepG2 cells for 48 h were 458.10 mg/mL and 3.2 mg/mL, respectively. Moreover, apoptotic cells were determined by AO/EB (acridine orange/ethidium bromide) fluorescent staining method and Annexin V-FITC/PI staining flow cytometry cell cycle analysis. With increasing of amygdalin concentration and the incubation time, the apoptotic rate was heightened. Compared with the control, there was significant difference (p<0.01). Together, these findings indicate that amygdalin had no strong anti-HepG2 activity; however the ingredients of amygdalin activated with β-D-glucosidase had a higher and efficient anti-HepG2 activity. It was therefore suggested that this combination strategy may be applicable for treating tumors with a higher activity.
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, China; College of Animal Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, China; School of Agriculture and Food Science, Zhejiang A & F University, 88 Huancheng Road (N), Lin'an 311300, China; Jiangsu Provincial Research Center of Bio-process and Separation Engineering of Agri-products, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, China; Key Laboratory for Physical Processing of Agricultural Products, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, China.
authors.forename: Cunshan
authors.initials: C
authors.lastname: Zhou
authors.affiliation: College of Animal Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, China.
authors.forename: Lichun
authors.initials: L
authors.lastname: Qian
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, China; Jiangsu Provincial Research Center of Bio-process and Separation Engineering of Agri-products, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, China; Key Laboratory for Physical Processing of Agricultural Products, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, China.
authors.forename: Haile
authors.initials: H
authors.lastname: Ma
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, China; School of Agriculture and Food Science, Zhejiang A & F University, 88 Huancheng Road (N), Lin'an 311300, China; Jiangsu Provincial Research Center of Bio-process and Separation Engineering of Agri-products, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, China; Key Laboratory for Physical Processing of Agricultural Products, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, China.
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Yu
authors.affiliation: School of Agriculture and Food Science, Zhejiang A & F University, 88 Huancheng Road (N), Lin'an 311300, China.
authors.forename: Youzuo
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, China; Jiangsu Provincial Research Center of Bio-process and Separation Engineering of Agri-products, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, China; Key Laboratory for Physical Processing of Agricultural Products, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, China.
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Qu
authors.affiliation: College of Animal Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, China.
authors.forename: Xiaoxu
authors.initials: X
authors.lastname: Zhang
authors.affiliation: College of Animal Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Xia
mesh_terms: D000678:Amygdalin / Q000737:chemistry* / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects* / Q000502:physiology; D056945:Hep G2 Cells; D006801:Humans; D001617:beta-Glucosidase / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D000678:Amygdalin; D001617:beta-Glucosidase