title: A cell type-specific view on the translation of mRNAs from ROS-responsive genes upon paraquat treatment of Arabidopsis thaliana leaves.
abstract: Oxidative stress causes dramatic changes in the expression levels of many genes. The formation of a functional protein through successful mRNA translation is central to a coordinated cellular response. To what extent the response towards reactive oxygen species (ROS) is regulated at the translational level is poorly understood. Here we analysed leaf- and tissue-specific translatomes using a set of transgenic Arabidopsis thaliana lines expressing a FLAG-tagged ribosomal protein to immunopurify polysome-bound mRNAs before and after oxidative stress. We determined transcript levels of 171 ROS-responsive genes upon paraquat treatment, which causes formation of superoxide radicals, at the whole-organ level. Furthermore, the translation of mRNAs was determined for five cell types: mesophyll, bundle sheath, phloem companion, epidermal and guard cells. Mesophyll and bundle sheath cells showed the strongest response to paraquat treatment. Interestingly, several ROS-responsive transcription factors displayed cell type-specific translation patterns, while others were translated in all cell types. In part, cell type-specific translation could be explained by the length of the 5'-untranslated region (5'-UTR) and the presence of upstream open reading frames (uORFs). Our analysis reveals insights into the translational regulation of ROS-responsive genes, which is important to understanding cell-specific responses and functions during oxidative stress.
authors.affiliation: Department of Plant Physiology and Molecular Biology, University of Plovdiv, 4000, Plovdiv, Bulgaria; Institute of Molecular Biology and Biotechnology, 4000, Plovdiv, Bulgaria.
authors.forename: Maria
authors.initials: M
authors.lastname: Benina
authors.forename: Dimas Mendes
authors.initials: DM
authors.lastname: Ribeiro
authors.forename: Tsanko S
authors.initials: TS
authors.lastname: Gechev
authors.forename: Bernd
authors.initials: B
authors.lastname: Mueller-Roeber
authors.forename: Jos H M
authors.initials: JH
authors.lastname: Schippers
mesh_terms: D020121:5' Untranslated Regions / Q000235:genetics; D017360:Arabidopsis / Q000166:cytology* / Q000187:drug effects / Q000235:genetics*; D001483:Base Sequence; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant; D008969:Molecular Sequence Data; D009711:Nucleotides / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D010269:Paraquat / Q000633:toxicity*; D018515:Plant Leaves / Q000166:cytology* / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D011132:Polyribosomes / Q000187:drug effects / Q000378:metabolism; D014176:Protein Biosynthesis / Q000187:drug effects*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D020121:5' Untranslated Regions; D009711:Nucleotides; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D014157:Transcription Factors; D010269:Paraquat
keywords: Arabidopsis; gene regulation; oxidative stress; tissue-specific; translation