title: [Study on bone mesenchymal stem cells transfected by polyethylene glycol/bone morphogenetic protein-2].
abstract: OBJECTIVE
  Polyethylene glycol/bone morphogenetic protein-2 (PEG/BMP-2) nanoparticles were transfected into Rabbit bone mesenchymal stem cells (rBMSCs) and the expression of BMP-2 was detected.
  
  
  METHODS
  Dissociated rBMSCs were primarily cultured in vitro and BMP-2 gene was transfected into rBMSCs by PEG/BMP-2 nanoparticals and lipofectamine, respectively. The efficiency of transfection was detected by flow cytometry and the expression of BMP-2 was detected by Western Blot and real time RT-PCR.
  
  
  RESULTS
  PEG/BMP-2 nanoparticals were successfully synthesized and transfected into rBMSCs. Compared with the lipofectamine transfection group, PEG/BMP-2 transfection group had higher efficiency and higher BMP-2 expression.
  
  
  CONCLUSIONS
  PEG/BMP-2 nanoparticals transfected rBMSCs highly expressed BMP-2,which provided novel strategies for the treatment of bone defect.
authors.forename: Li-Feng
authors.initials: LF
authors.lastname: Ding
authors.forename: Gang
authors.initials: G
authors.lastname: Zheng
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.forename: Zhen-Dong
authors.initials: ZD
authors.lastname: Zhou
authors.forename: Jian-Jun
authors.initials: JJ
authors.lastname: Li
mesh_terms: D000818:Animals; D001847:Bone Diseases / Q000235:genetics / Q000628:therapy; D055396:Bone Morphogenetic Protein 2 / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D001842:Bone and Bones / Q000166:cytology*; D005786:Gene Expression Regulation; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D053758:Nanoparticles / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011817:Rabbits; D014162:Transfection / Q000379:methods*
chemical_list: D055396:Bone Morphogenetic Protein 2; D012333:RNA, Messenger; D011092:Polyethylene Glycols