title: Regulation of adipocytes lipolysis by n-3 HUFA in grass carp (Ctenopharyngodon idellus) in vitro and in vivo.
abstract: N-3 highly unsaturated fatty acids (n-3 HUFA) have been shown to inhibit body fat accumulation in animals. To clarify the mechanism of this fat-lowering effect of n-3 HUFA in grass carp (Ctenopharyngodon idellus), two experiments were conducted. In experiment 1, isolated grass carp mature adipocytes were incubated with docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) at different concentrations for 6 h. The release of glycerol to the medium was detected, and the expression of the lipolysis-related genes was analyzed. In experiment 2, a 95-day feeding trial was conducted with two diets formulated with either lard oil (as control) or fish oil (supplying n-3 HUFA as treatment) as the main lipid source. The glycerol and free fatty acid (FFA) released from the isolated adipocytes of both groups were detected after the feeding period. The expression of select lipolysis-related genes in adipose tissue was also analyzed. The results from experiment 1 showed that the release of glycerol was significantly increased by DHA and EPA (P < 0.05). Moreover, the expression of lipolysis-related genes, such as adipose triglyceride lipase (ATGL), hormone-sensitive lipase (HSL), tumor necrosis factor α (TNFα) and leptin, was also significantly elevated in the treatment group (P < 0.05). Experiment 2 demonstrated that glycerol and FFA release from the isolated adipocytes were significantly higher in the treatment group compared to the control group (P < 0.05). The expression level of ATGL, HSL, TNFα and leptin in the treatment group was significantly higher than in the control group (P < 0.05). The present results provide novel evidence that n-3 HUFAs could regulate grass carp adipocyte lipolysis in vitro or in vivo, and the effect might be in part associated with their influence on the expression of lipolysis-related genes and lipolysis-related adipokines genes.
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, China.
authors.forename: Pin
authors.initials: P
authors.lastname: Liu
authors.forename: Chao
authors.initials: C
authors.lastname: Li
authors.forename: Jiqin
authors.initials: J
authors.lastname: Huang
authors.forename: Hong
authors.initials: H
authors.lastname: Ji
mesh_terms: D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism*; D000704:Analysis of Variance; D000818:Animals; D002347:Carps / Q000378:metabolism*; D017931:DNA Primers / Q000235:genetics; D004281:Docosahexaenoic Acids; D015118:Eicosapentaenoic Acid; D005230:Fatty Acids, Nonesterified / Q000097:blood; D015525:Fatty Acids, Omega-3 / Q000494:pharmacology*; D020869:Gene Expression Profiling / Q000662:veterinary; D005990:Glycerol / Q000097:blood; D020738:Leptin / Q000378:metabolism; D008049:Lipase / Q000378:metabolism; D008066:Lipolysis / Q000187:drug effects* / Q000502:physiology; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D002787:Sterol Esterase / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D017931:DNA Primers; D005230:Fatty Acids, Nonesterified; D015525:Fatty Acids, Omega-3; D020738:Leptin; D014409:Tumor Necrosis Factor-alpha; D004281:Docosahexaenoic Acids; D015118:Eicosapentaenoic Acid; D002787:Sterol Esterase; D008049:Lipase; D005990:Glycerol
references.citation: Biochem Biophys Res Commun. 2004 May 21;318(1):168-75
references.pmid: 15110769
references.citation: Lipids. 2004 Dec;39(12):1177-85
references.pmid: 15736913
references.citation: Prog Lipid Res. 2009 Sep;48(5):275-97
references.pmid: 19464318
references.citation: J Nutr Biochem. 2010 May;21(5):357-63
references.pmid: 20149625
references.citation: Physiol Res. 2006;55(1):63-72
references.pmid: 15857162
references.citation: Obes Res. 1995 Dec;3 Suppl 5:633S-636S
references.pmid: 8653540
references.citation: Am J Physiol Endocrinol Metab. 2002 Feb;282(2):E318-25
references.pmid: 11788363
references.citation: Trends Endocrinol Metab. 2008 Jan;19(1):3-9
references.pmid: 18155916
references.citation: PLoS One. 2012;7(10):e48069
references.pmid: 23110176
references.citation: Comp Biochem Physiol B Biochem Mol Biol. 2009 Feb;152(2):135-43
references.pmid: 19010438
references.citation: J Lipid Res. 1971 Jan;12(1):65-70
references.pmid: 4322518
references.citation: Clin Endocrinol (Oxf). 2006 Apr;64(4):355-65
references.pmid: 16584505
references.citation: PLoS One. 2010 Apr 01;5(4):e9997
references.pmid: 20376319
references.citation: Diabetes Metab. 1998 Nov;24(5):409-18
references.pmid: 9881238
references.citation: PLoS One. 2012;7(7):e40876
references.pmid: 22815850
references.citation: J Physiol. 2007 Jul 1;582(Pt 1):349-58
references.pmid: 17510185
references.citation: Biochem Biophys Res Commun. 2000 Apr 13;270(2):343-8
references.pmid: 10753628
references.citation: Apoptosis. 2003 Aug;8(4):327-35
references.pmid: 12815275
references.citation: Lipids. 2003 Mar;38(3):289-96
references.pmid: 12784870
references.citation: Biochim Biophys Acta. 2009 Jun;1791(6):459-66
references.pmid: 19041421
references.citation: J Cell Biochem. 2003 Aug 15;89(6):1077-86
references.pmid: 12898507
references.citation: J Nutr Biochem. 2010 Apr;21(4):317-24
references.pmid: 19369048
references.citation: Essays Biochem. 2006;42:89-103
references.pmid: 17144882
references.citation: Diabetes. 2002 Oct;51(10):2929-35
references.pmid: 12351429
references.citation: J Appl Physiol (1985). 1999 Jul;87(1):227-32
references.pmid: 10409579
references.citation: Best Pract Res Clin Endocrinol Metab. 2005 Dec;19(4):471-82
references.pmid: 16311212
references.citation: J Lipid Res. 2001 May;42(5):743-50
references.pmid: 11352981
references.citation: Proc Nutr Soc. 1993 Oct;52(3):417-26
references.pmid: 8302883
references.citation: J Clin Endocrinol Metab. 2004 Jun;89(6):2595-600
references.pmid: 15181029
references.citation: Am J Physiol Regul Integr Comp Physiol. 2005 Jul;289(1):R259-65
references.pmid: 15746305
references.citation: Prog Lipid Res. 2003 Jul;42(4):257-88
references.pmid: 12689620
references.citation: Biochem Biophys Res Commun. 1997 Nov 26;240(3):590-4
references.pmid: 9398609
references.citation: Diabetologia. 2009 Mar;52(3):541-6
references.pmid: 19048227
references.citation: J Biol Chem. 2006 Jun 9;281(23):15837-44
references.pmid: 16595669
references.citation: Biochem Biophys Res Commun. 2010 Sep 10;400(1):134-9
references.pmid: 20708600
references.citation: J Nutr. 2000 Dec;130(12):3110S-3115S
references.pmid: 11110881
references.citation: J Lipid Res. 2007 Dec;48(12):2547-59
references.pmid: 17878492
references.citation: Comp Biochem Physiol B Biochem Mol Biol. 2011 May;159(1):49-56
references.pmid: 21296179
references.citation: J Biol Chem. 1999 Mar 5;274(10):6770-5
references.pmid: 10037777
references.citation: J Lipid Res. 1994 Feb;35(2):177-93
references.pmid: 8169522
references.citation: Am J Physiol Endocrinol Metab. 2003 Sep;285(3):E521-6
references.pmid: 12736159
references.citation: Comp Biochem Physiol B Biochem Mol Biol. 2006 Jul;144(3):326-33
references.pmid: 16716627
references.citation: Comp Biochem Physiol A Mol Integr Physiol. 2005 Nov;142(3):347-54
references.pmid: 16213761
references.citation: J Nutr. 2006 Dec;136(12):2965-9
references.pmid: 17116704
references.citation: Am J Physiol Regul Integr Comp Physiol. 2005 Jun;288(6):R1682-8
references.pmid: 15650121
references.citation: Annu Rev Nutr. 2007;27:79-101
references.pmid: 17313320
references.citation: Genes Nutr. 2008 Feb;2(4):327-30
references.pmid: 18850226
references.citation: Prog Lipid Res. 2011 Jan;50(1):14-27
references.pmid: 21087632
references.citation: J Lipid Res. 2007 May;48(5):1078-89
references.pmid: 17308334
references.citation: Annu Rev Nutr. 1989;9:417-43
references.pmid: 2669880
references.citation: Comp Biochem Physiol B Biochem Mol Biol. 2010 Aug;156(4):309-18
references.pmid: 20433939
references.citation: Cardiovasc Pathol. 2011 Sep-Oct;20(5):e153-6
references.pmid: 20829073
references.citation: Lipids. 2008 Sep;43(9):813-27
references.pmid: 18615261
references.citation: Fish Physiol Biochem. 2012 Dec;38(6):1759-1774
references.pmid: 22760861
references.citation: Int J Obes Relat Metab Disord. 2003 Oct;27(10):1178-86
references.pmid: 14513065
references.citation: Lipids Health Dis. 2010 Jun 04;9:57
references.pmid: 20525346
references.citation: Lipids. 2011 Sep;46(9):821-30
references.pmid: 21643838
references.citation: Science. 2004 Nov 19;306(5700):1383-6
references.pmid: 15550674
references.citation: J Cell Biochem. 2008 Dec 15;105(6):1430-6
references.pmid: 18980248
references.citation: J Biol Chem. 2003 Mar 7;278(10):8401-6
references.pmid: 12477720
references.citation: Diabetes Care. 1998 May;21(5):717-24
references.pmid: 9589230