title: Molecular characterization of Klebsiella pneumoniae isolated from renal transplanted patients: virulence markers, extended-spectrum β-lactamases, and genetic relatedness.
abstract: The objective was to characterize virulence markers and β-lactam resistance in Klebsiella pneumoniae isolates from renal transplant patients and to evaluate their genetic relatedness. Two main genetic lineages were detected: 1 carried bla(CTX-M-15) not associated to IncFIIA plasmid replicon, which was found on the other lineage not expressing CTX-M-type enzyme. PAI III(536) and PAI II(CFT073) were detected for the first time in K. pneumoniae in 1 clone, while the siderophore kfu was carried by the other, with only PAI IV(536). The molecular data indicate colonization before admission and fuel the discussion on implementation of antibiotherapy before surgery.
authors.affiliation: Center of Pharmaceutical Studies and Laboratory of Microbiology, Faculty of Pharmacy, University of Coimbra, Coimbra, Portugal; Service of Clinical Pathology, University Hospital of Coimbra, Coimbra, Portugal.
authors.forename: Vera
authors.initials: V
authors.lastname: Calhau
authors.affiliation: Service of Clinical Pathology, University Hospital of Coimbra, Coimbra, Portugal.
authors.forename: Luísa
authors.initials: L
authors.lastname: Boaventura
authors.affiliation: Service of Clinical Pathology, University Hospital of Coimbra, Coimbra, Portugal.
authors.forename: Graça
authors.initials: G
authors.lastname: Ribeiro
authors.affiliation: Center of Pharmaceutical Studies and Laboratory of Microbiology, Faculty of Pharmacy, University of Coimbra, Coimbra, Portugal.
authors.forename: Nuno
authors.initials: N
authors.lastname: Mendonça
authors.affiliation: Center of Pharmaceutical Studies and Laboratory of Microbiology, Faculty of Pharmacy, University of Coimbra, Coimbra, Portugal. Electronic address: gjsilva@ci.uc.pt.
authors.forename: Gabriela J
authors.initials: GJ
authors.lastname: da Silva
mesh_terms: D006801:Humans; D016030:Kidney Transplantation; D007710:Klebsiella Infections / Q000382:microbiology*; D007711:Klebsiella pneumoniae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D058889:Molecular Typing; D010957:Plasmids; D066027:Transplant Recipients; D037521:Virulence Factors / Q000235:genetics*; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D037521:Virulence Factors; D001618:beta-Lactamases
keywords: CTX-M-15; Pathogenicity island; Plasmid replicon typing; Virulence factors