title: Bioproduction of 4-vinylphenol from corn cob alkaline hydrolyzate in two-phase extractive fermentation using free or immobilized recombinant E. coli expressing pad gene.
abstract: In situ extractive fermentation was used to produce 4-vinyl derivatives from hydroxycinnamic acids extracted from corn cobs by recombinant Escherichia coli cells expressing Lactobacillus plantarum phenolic acid descarboxylase (PAD) gene. This microorganism mainly produced 4-vinylphenol (4VP) from p-coumaric acid (p-CA). In the first study , we observed that the concentrations of 4VP are higher than 1g/L which had a negative impact on decarboxylation of p-CA to 4VP by recombinant E. coli cells. Because of this, and in order to improve the downstream process, a two-phase aqueous-organic solvent system was developed. The results of the extractive fermentation indicated that it was possible to use hydrolyzates as aqueous phase to bioproduce 4VP, and recover simultaneously the product in the organic phase containing hexane. The detoxification of pre-treated corn cob alkaline hydrolyzate improved 4VP production up to 1003.5mg/L after 24h fermentation (QP=41.813mg/Lh). Additionally, preliminary experiments using cells immobilized in calcium alginate showed to be a good system for the biotransform of p-CA to 4VP in extractive fermentation, although the process hindered partially the recovery of 4VP in the organic phase.
authors.affiliation: IBB-Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal. Electronic address: jmsalgado@uvigo.es.
authors.forename: José Manuel
authors.initials: JM
authors.lastname: Salgado
authors.affiliation: Laboratory of Agro-food Biotechnology, CITI-Tecnólopole, Parque Tecnológico de Galicia, San Cibrao das Viñas, Ourense, Spain; Department of Chemical Engineering, Sciences Faculty, University of Vigo (Campus Ourense), As Lagoas s/n, 32004 Ourense, Spain.
authors.forename: Raquel
authors.initials: R
authors.lastname: Rodríguez-Solana
authors.affiliation: Laboratorio y Tecnología de Alimentos y Nutrición, ICTAN-CSIC, Juan de la Cierva 3, 28006 Madrid, de Biotecnología Bacteriana, Instituto de Ciencia, Spain.
authors.forename: José Antonio
authors.initials: JA
authors.lastname: Curiel
authors.affiliation: Laboratorio y Tecnología de Alimentos y Nutrición, ICTAN-CSIC, Juan de la Cierva 3, 28006 Madrid, de Biotecnología Bacteriana, Instituto de Ciencia, Spain.
authors.forename: Blanca
authors.initials: B
authors.lastname: de Las Rivas
authors.affiliation: Laboratorio y Tecnología de Alimentos y Nutrición, ICTAN-CSIC, Juan de la Cierva 3, 28006 Madrid, de Biotecnología Bacteriana, Instituto de Ciencia, Spain.
authors.forename: Rosario
authors.initials: R
authors.lastname: Muñoz
authors.affiliation: Laboratory of Agro-food Biotechnology, CITI-Tecnólopole, Parque Tecnológico de Galicia, San Cibrao das Viñas, Ourense, Spain; Department of Chemical Engineering, Sciences Faculty, University of Vigo (Campus Ourense), As Lagoas s/n, 32004 Ourense, Spain.
authors.forename: José Manuel
authors.initials: JM
authors.lastname: Domínguez
mesh_terms: D000464:Alginates; D001426:Bacterial Proteins / Q000378:metabolism*; D002262:Carboxy-Lyases / Q000378:metabolism*; D018914:Cells, Immobilized / Q000378:metabolism*; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003373:Coumaric Acids / Q000378:metabolism*; D003470:Culture Media; D004926:Escherichia coli / Q000378:metabolism*; D005285:Fermentation; D005561:Formates; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D048191:Lactiplantibacillus plantarum / Q000201:enzymology* / Q000235:genetics; D000432:Methanol; D008863:Microspheres; D010636:Phenols / Q000302:isolation & purification* / Q000378:metabolism; D011422:Propionates; D011994:Recombinant Proteins / Q000378:metabolism; D012997:Solvents; D003313:Zea mays / Q000378:metabolism*
chemical_list: D000464:Alginates; D001426:Bacterial Proteins; D003373:Coumaric Acids; D003470:Culture Media; D005561:Formates; D006603:Hexuronic Acids; D010636:Phenols; D011422:Propionates; D011994:Recombinant Proteins; D012997:Solvents; C030544:formic acid; D020723:Glucuronic Acid; D002262:Carboxy-Lyases; C417714:phenolic acid decarboxylase; C495469:p-coumaric acid; C030626:4-vinylphenol; D000432:Methanol
keywords: 4-Vinylphenol; Corn cobs; Extractive fermentation; Immobilized cells