title: Efficacy of gallium maltolate against Lawsonia intracellularis infection in a rabbit model.
abstract: Antimicrobial efficacy against Lawsonia intracellularis is difficult to evaluate in vitro, thus, the effects of gallium maltolate's (GaM) were investigated in a rabbit model for equine proliferative enteropathy (EPE). Juvenile (5-6-week-old) does were infected with 3.0 × 10(8) L. intracellularis/rabbit and allocated into three groups (n = 8). One week postinfection, one group was treated with GaM, 50 mg/kg; one, with doxycycline, 5 mg/kg; and one with a sham-treatment (control). Feces and blood were collected daily and weekly, respectively, to verify presence of L. intracellularis fecal shedding using qPCR, and seroconversion using immunoperoxidase monolayer assay. Rabbits were sacrificed after 1 week of treatment to collect intestinal tissues focusing on EPE-affected sections. Intestinal lesions were confirmed via immunohistochemistry. No difference was noted between treatments regarding EPE-lesions in jejunum (P = 0.51), ileum (P = 0.74), and cecum (P = 0.35), or in L. intracellularis fecal shedding (P = 0.64). GaM and doxycycline appear to have similar efficacy against EPE in infected rabbits.
authors.affiliation: Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK, Canada.
authors.forename: F
authors.initials: F
authors.lastname: Sampieri
authors.forename: A L
authors.initials: AL
authors.lastname: Allen
authors.forename: J
authors.initials: J
authors.lastname: Alcorn
authors.forename: C R
authors.initials: CR
authors.lastname: Clark
authors.forename: F A
authors.initials: FA
authors.lastname: Vannucci
authors.forename: N
authors.initials: N
authors.lastname: Pusterla
authors.forename: S M
authors.initials: SM
authors.lastname: Mapes
authors.forename: K R
authors.initials: KR
authors.lastname: Ball
authors.forename: P M
authors.initials: PM
authors.lastname: Dowling
authors.forename: J
authors.initials: J
authors.lastname: Thompson
authors.forename: L R
authors.initials: LR
authors.lastname: Bernstein
authors.forename: C J
authors.initials: CJ
authors.lastname: Gebhart
authors.forename: D L
authors.initials: DL
authors.lastname: Hamilton
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000627:therapeutic use*; D045824:Desulfovibrionaceae Infections / Q000188:drug therapy / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D004195:Disease Models, Animal; D005260:Female; D020639:Lawsonia Bacteria / Q000187:drug effects*; D009942:Organometallic Compounds / Q000627:therapeutic use*; D011753:Pyrones / Q000627:therapeutic use*; D011817:Rabbits; D016896:Treatment Outcome
chemical_list: D000900:Anti-Bacterial Agents; D009942:Organometallic Compounds; D011753:Pyrones; C512662:gallium maltolate