title: Prevalence of type III secretion system in effective biocontrol pseudomonads.
abstract: Functional type III secretion system (T3SS) genes are needed for effective biocontrol of Pythium damping-off of cucumber by Pseudomonas fluorescens KD, but whether biocontrol Pseudomonas strains with T3SS genes display overall a higher plant-protecting activity is unknown. The assessment of 198 biocontrol fluorescent pseudomonads originating from 60 soils worldwide indicated that 32% harbour the ATPase-encoding T3SS gene hrcN, which was most often found in tomato isolates. The hrcN(+) biocontrol strains (and especially those also producing 2,4-diacetylphloroglucinol and displaying 1-aminocyclopropane-1-carboxylate deaminase activity) displayed higher plant-protecting ability in comparison with hrcN(-) biocontrol strains, both in the Pythium/cucumber and Fusarium/cucumber pathosystems.
authors.affiliation: Université de Lyon, F-69622 Lyon, France; Université Lyon 1, Villeurbanne, France; CNRS, UMR5557, Ecologie Microbienne, Villeurbanne, France.
authors.forename: Juliana
authors.initials: J
authors.lastname: Almario
authors.affiliation: Phytopathology Group, Institute of Integrative Biology, Swiss Federal Institute of Technology (ETH), Universitätstrasse 2, CH-8092 Zürich, Switzerland; Department of Sustainable Agro-Ecosystems and Bioresources, IASMA Research and Innovation Centre, Fondazione Edmund Mach (FEM), I-38010 S. Michele all'Adige, Italy.
authors.forename: Davide
authors.initials: D
authors.lastname: Gobbin
authors.affiliation: Phytopathology Group, Institute of Integrative Biology, Swiss Federal Institute of Technology (ETH), Universitätstrasse 2, CH-8092 Zürich, Switzerland.
authors.forename: Geneviève
authors.initials: G
authors.lastname: Défago
authors.affiliation: Université de Lyon, F-69622 Lyon, France; Université Lyon 1, Villeurbanne, France; CNRS, UMR5557, Ecologie Microbienne, Villeurbanne, France. Electronic address: yvan.moenne-loccoz@univ-lyon1.fr.
authors.forename: Yvan
authors.initials: Y
authors.lastname: Moënne-Loccoz
authors.affiliation: Department of Sustainable Agro-Ecosystems and Bioresources, IASMA Research and Innovation Centre, Fondazione Edmund Mach (FEM), I-38010 S. Michele all'Adige, Italy; Research Group Environmental Genomics and Systems Biology, Institute of Natural Resource Sciences, Zurich University for Applied Sciences (ZHAW), Campus Grüental, P.O. Box, CH-8820 Wädenswil, Switzerland. Electronic address: rezz@zhaw.ch.
authors.forename: Fabio
authors.initials: F
authors.lastname: Rezzonico
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D058947:Bacterial Secretion Systems; D018553:Cucumis sativus / Q000382:microbiology; D018551:Solanum lycopersicum / Q000382:microbiology; D010935:Plant Diseases / Q000382:microbiology* / Q000517:prevention & control; D011551:Pseudomonas fluorescens / Q000201:enzymology* / Q000235:genetics; D011775:Pythium / Q000502:physiology*; D012988:Soil Microbiology
chemical_list: D001426:Bacterial Proteins; D058947:Bacterial Secretion Systems; D000251:Adenosine Triphosphatases
keywords: 2,4-Diacetylphloroglucinol; ACC deaminase; Biocontrol; Pseudomonas; Type III secretion system