title: The ARC1 E3 Ligase Promotes Two Different Self-Pollen Avoidance Traits in Arabidopsis.
abstract: Flowering plants have evolved various strategies for avoiding self-pollen to drive genetic diversity. These strategies include spatially separated sexual organs (herkogamy), timing differences between male pollen release and female pistil receptivity (dichogamy), and self-pollen rejection. Within the Brassicaceae, these outcrossing systems are the evolutionary default state, and many species display these traits, including Arabidopsis lyrata. In contrast to A. lyrata, closely related Arabidopsis thaliana has lost these self-pollen traits and thus represents an excellent system to test genes for reconstructing these evolutionary traits. We previously demonstrated that the ARC1 E3 ligase is required for self-incompatibility in two diverse Brassicaceae species, Brassica napus and A. lyrata, and is frequently deleted in self-compatible species, including A. thaliana. In this study, we examined ARC1's requirement for reconstituting self-incompatibility in A. thaliana and uncovered an important role for ARC1 in promoting a strong and stable pollen rejection response when expressed with two other A. lyrata self-incompatibility factors. Furthermore, we discovered that ARC1 promoted an approach herkogamous phenotype in A. thaliana flowers. Thus, ARC1's expression resulted in two different A. lyrata traits for self-pollen avoidance and highlights the key role that ARC1 plays in the evolution and retention of outcrossing systems.
authors.affiliation: Department of Cell and Systems Biology, University of Toronto, Toronto M5S 3B2, Canada.
authors.forename: Emily
authors.initials: E
authors.lastname: Indriolo
authors.affiliation: Department of Cell and Systems Biology, University of Toronto, Toronto M5S 3B2, Canada.
authors.forename: Darya
authors.initials: D
authors.lastname: Safavian
authors.affiliation: Department of Cell and Systems Biology, University of Toronto, Toronto M5S 3B2, Canada Centre for the Analysis of Genome Evolution and Function, University of Toronto, Toronto M5S 3B2, Canada d.goring@utoronto.ca.
authors.forename: Daphne R
authors.initials: DR
authors.lastname: Goring
references.citation: Nature. 2010 Apr 29;464(7293):1342-6
references.pmid: 20400945
references.citation: Science. 1999 Nov 26;286(5445):1729-31
references.pmid: 10576738
references.citation: Plant Methods. 2009 Feb 27;5:3
references.pmid: 19250520
references.citation: Science. 2007 Aug 24;317(5841):1070-2
references.pmid: 17656687
references.citation: Traffic. 2012 Jul;13(7):898-907
references.pmid: 22420621
references.citation: Proc Natl Acad Sci U S A. 2004 Nov 9;101(45):16070-4
references.pmid: 15505209
references.citation: Plant Cell. 2003 Apr;15(4):885-98
references.pmid: 12671085
references.citation: Trends Plant Sci. 2012 Oct;17(10):606-15
references.pmid: 22784825
references.citation: Genetics. 2006 Jan;172(1):477-84
references.pmid: 16157671
references.citation: BMC Struct Biol. 2008 May 16;8:26
references.pmid: 18485199
references.citation: Plant J. 1998 Dec;16(6):735-43
references.pmid: 10069079
references.citation: Plant Cell. 2001 Mar;13(3):627-43
references.pmid: 11251101
references.citation: Proc Natl Acad Sci U S A. 2009 Mar 31;106(13):5246-51
references.pmid: 19307580
references.citation: Plant Cell. 2007 Dec;19(12):3961-73
references.pmid: 18065692
references.citation: Mol Cell. 2005 Nov 23;20(4):525-38
references.pmid: 16307917
references.citation: Proc Natl Acad Sci U S A. 2011 Nov 1;108(44):18173-8
references.pmid: 22025723
references.citation: Mol Biol Evol. 2006 Sep;23(9):1741-50
references.pmid: 16782760
references.citation: Plant Cell. 2009 Sep;21(9):2642-54
references.pmid: 19767457
references.citation: Science. 2002 Jul 12;297(5579):247-9
references.pmid: 12114625
references.citation: Trends Plant Sci. 2011 Feb;16(2):108-16
references.pmid: 21177137
references.citation: Plant Cell. 2011 Sep;23(9):3156-71
references.pmid: 21954462
references.citation: Plant Cell. 2009 Sep;21(9):2655-71
references.pmid: 19789280
references.citation: Science. 2001 Sep 7;293(5536):1824-6
references.pmid: 11546871
references.citation: Genetics. 2001 May;158(1):387-99
references.pmid: 11333247
references.citation: Plant Cell Rep. 2007 Jun;26(6):745-53
references.pmid: 17205335
references.citation: J Exp Bot. 2010 Apr;61(7):1897-906
references.pmid: 20097845
references.citation: Plant Cell. 1990 Aug;2(8):755-67
references.pmid: 2152125
references.citation: Nature. 2001 Oct 4;413(6855):534-8
references.pmid: 11586363
references.citation: PLoS One. 2013 Dec 26;8(12):e84286
references.pmid: 24386363
references.citation: Evolution. 2012 May;66(5):1360-74
references.pmid: 22519777
references.citation: Curr Biol. 2006 Sep 5;16(17):R726-35
references.pmid: 16950099
references.citation: Plant Physiol. 2004 Jan;134(1):59-66
references.pmid: 14657406
references.citation: J Integr Plant Biol. 2010 Feb;52(2):138-46
references.pmid: 20377676
references.citation: J Exp Bot. 2010 Apr;61(7):1987-99
references.pmid: 20181663
references.citation: Heredity (Edinb). 2006 Oct;97(4):283-90
references.pmid: 16773120
references.citation: Biochemistry. 2010 Jan 19;49(2):347-55
references.pmid: 20017557
references.citation: Traffic. 2005 Jan;6(1):56-65
references.pmid: 15569245
references.citation: PLoS Genet. 2009 Mar;5(3):e1000426
references.pmid: 19300485
references.citation: Mol Ecol. 2007 Sep;16(17):3565-80
references.pmid: 17845431
references.citation: Plant Cell. 2012 Nov;24(11):4607-20
references.pmid: 23204404
references.citation: Proc Natl Acad Sci U S A. 1998 Jan 6;95(1):382-7
references.pmid: 9419384
references.citation: Mol Biol Evol. 2005 Jul;22(7):1609-20
references.pmid: 15858208
references.citation: Plant J. 2005 Jul;43(1):153-63
references.pmid: 15960624
references.citation: Plant Cell. 2010 Dec;22(12):4009-30
references.pmid: 21193573
references.citation: PLoS Biol. 2005 Jul;3(7):e196
references.pmid: 15907155
references.citation: Nat Cell Biol. 2008 May;10(5):505-7
references.pmid: 18454128
references.citation: Genetics. 2009 Aug;182(4):1313-21
references.pmid: 19506308
references.citation: Curr Opin Plant Biol. 2012 Feb;15(1):78-83
references.pmid: 21968124
references.citation: Mol Ecol. 2008 Jan;17(2):704-14
references.pmid: 18179433
references.citation: Trends Plant Sci. 2010 Jul;15(7):387-94
references.pmid: 20621670
references.citation: PLoS One. 2013;8(2):e57902
references.pmid: 23469099
references.citation: Plant Cell Physiol. 2012 Aug;53(8):1355-65
references.pmid: 22764279
references.citation: Plant Physiol. 2011 Oct;157(2):937-46
references.pmid: 21810962
references.citation: Philos Trans R Soc Lond B Biol Sci. 2003 Jun 29;358(1434):991-1004
references.pmid: 12831464
references.citation: Plant Cell. 2007 Jan;19(1):107-17
references.pmid: 17220204
references.citation: Biol Cell. 2006 Jan;98(1):53-67
references.pmid: 16354162
references.citation: Science. 2004 Mar 5;303(5663):1516-9
references.pmid: 15001779