title: PKA regulatory subunit expression in tooth development.
abstract: Protein kinase A (PKA) plays critical roles in many biological processes including cell proliferation, cell differentiation, cellular metabolism and gene regulation. Mutation in PKA regulatory subunit, PRKAR1A has previously been identified in odontogenic myxomas, but it is unclear whether PKA is involved in tooth development. The aim of the present study was to assess the expression of alpha isoforms of PKA regulatory subunit (Prkar1a and Prkar2a) in mouse and human odontogenesis by in situ hybridization. PRKAR1A and PRKAR2A mRNA transcription was further confirmed in a human deciduous germ by qRT-PCR. Mouse Prkar1a and human PRKAR2A exhibited a dynamic spatio-temporal expression in tooth development, whereas neither human PRKAR1A nor mouse Prkar2a showed their expression in odontogenesis. These isoforms thus showed different expression pattern between human and mouse tooth germs.
authors.affiliation: Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil. Electronic address: silvinhaferreira21@yahoo.com.br.
authors.forename: Sílvia Ferreira
authors.initials: SF
authors.lastname: de Sousa
authors.affiliation: Department of Craniofacial Development and Stem Cell Biology, Dental Institute, King's College London, Tower Wing Guy's Hospital, London Bridge, London SE1 9RT, UK.
authors.forename: Katsushige
authors.initials: K
authors.lastname: Kawasaki
authors.affiliation: Department of Craniofacial Development and Stem Cell Biology, Dental Institute, King's College London, Tower Wing Guy's Hospital, London Bridge, London SE1 9RT, UK.
authors.forename: Maiko
authors.initials: M
authors.lastname: Kawasaki
authors.affiliation: Department of Craniofacial Development and Stem Cell Biology, Dental Institute, King's College London, Tower Wing Guy's Hospital, London Bridge, London SE1 9RT, UK.
authors.forename: Ana Angelova
authors.initials: AA
authors.lastname: Volponi
authors.affiliation: Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
authors.forename: Ricardo Santiago
authors.initials: RS
authors.lastname: Gomez
authors.affiliation: Department of Pathology, Biological Sciences Institute, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
authors.forename: Carolina Cavaliéri
authors.initials: CC
authors.lastname: Gomes
authors.affiliation: Department of Craniofacial Development and Stem Cell Biology, Dental Institute, King's College London, Tower Wing Guy's Hospital, London Bridge, London SE1 9RT, UK.
authors.forename: Paul T
authors.initials: PT
authors.lastname: Sharpe
authors.affiliation: Department of Craniofacial Development and Stem Cell Biology, Dental Institute, King's College London, Tower Wing Guy's Hospital, London Bridge, London SE1 9RT, UK; Division of Oral Anatomy, Department of Oral Biological Science, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Ohazama
mesh_terms: D000818:Animals; D054756:Cyclic AMP-Dependent Protein Kinase RIalpha Subunit / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D017403:In Situ Hybridization; D051379:Mice; D009805:Odontogenesis; D059368:RNA Isoforms / Q000235:genetics*; D014094:Tooth, Deciduous / Q000196:embryology
chemical_list: D054756:Cyclic AMP-Dependent Protein Kinase RIalpha Subunit; D059368:RNA Isoforms
keywords: Human; In situ hybridization; Mouse; PRKAR1A; PRKAR2A; Tooth development