title: Anandamide attenuates haloperidol-induced vacuous chewing movements in rats.
abstract: Antipsychotics may cause tardive dyskinesia in humans and orofacial dyskinesia in rodents. Although the dopaminergic system has been implicated in these movement disorders, which involve the basal ganglia, their underlying pathomechanisms remain unclear. CB1 cannabinoid receptors are highly expressed in the basal ganglia, and a potential role for endocannabinoids in the control of basal ganglia-related movement disorders has been proposed. Therefore, this study investigated whether CB1 receptors are involved in haloperidol-induced orofacial dyskinesia in rats. Adult male rats were treated for four weeks with haloperidol decanoate (38mg/kg, intramuscularly - i.m.). The effect of anandamide (6nmol, intracerebroventricularly - i.c.v.) and/or the CB1 receptor antagonist SR141716A (30μg, i.c.v.) on haloperidol-induced vacuous chewing movements (VCMs) was assessed 28days after the start of the haloperidol treatment. Anandamide reversed haloperidol-induced VCMs; SR141716A (30μg, i.c.v.) did not alter haloperidol-induced VCM per se but prevented the effect of anandamide on VCM in rats. These results suggest that CB1 receptors may prevent haloperidol-induced VCMs in rats, implicating CB1 receptor-mediated cannabinoid signaling in orofacial dyskinesia.
authors.affiliation: Programa de Pós-Graduação em Farmacologia, Universidade Federal de Santa Maria, RS, Brazil.
authors.forename: Jivago
authors.initials: J
authors.lastname: Röpke
authors.affiliation: Programa de Pós-Graduação em Farmacologia, Universidade Federal de Santa Maria, RS, Brazil.
authors.forename: Alcindo
authors.initials: A
authors.lastname: Busanello
authors.affiliation: Curso de Farmácia, Universidade Federal de Santa Maria, RS, Brazil.
authors.forename: Caroline Queiroz
authors.initials: CQ
authors.lastname: Leal
authors.affiliation: Programa de Pós-Graduação em Farmacologia, Universidade Federal de Santa Maria, RS, Brazil.
authors.forename: Elizete
authors.initials: E
authors.lastname: de Moraes Reis
authors.affiliation: Curso de Farmácia, Universidade Federal de Santa Maria, RS, Brazil.
authors.forename: Catiuscia Molz
authors.initials: CM
authors.lastname: de Freitas
authors.affiliation: Programa de Pós-Graduação em Farmacologia, Universidade Federal de Santa Maria, RS, Brazil.
authors.forename: Jardel Gomes
authors.initials: JG
authors.lastname: Villarinho
authors.affiliation: Programa de Pós-Graduação em Bioquímica Toxicológica, Universidade Federal de Santa Maria, RS, Brazil.
authors.forename: Fernanda Hernandes
authors.initials: FH
authors.lastname: Figueira
authors.affiliation: Programa de Pós-Graduação em Farmacologia, Universidade Federal de Santa Maria, RS, Brazil.
authors.forename: Carlos Fernando
authors.initials: CF
authors.lastname: Mello
authors.affiliation: Programa de Pós-Graduação em Farmacologia, Universidade Federal de Santa Maria, RS, Brazil; Programa de Pós-Graduação em Bioquímica Toxicológica, Universidade Federal de Santa Maria, RS, Brazil.
authors.forename: Juliano
authors.initials: J
authors.lastname: Ferreira
authors.affiliation: Programa de Pós-Graduação em Farmacologia, Universidade Federal de Santa Maria, RS, Brazil; Programa de Pós-Graduação em Bioquímica Toxicológica, Universidade Federal de Santa Maria, RS, Brazil. Electronic address: roseleirf@gmail.com.br.
authors.forename: Roselei
authors.initials: R
authors.lastname: Fachinetto
mesh_terms: D000818:Animals; D014150:Antipsychotic Agents / Q000009:adverse effects*; D001095:Arachidonic Acids / Q000494:pharmacology*; D063386:Cannabinoid Receptor Agonists / Q000494:pharmacology*; D063387:Cannabinoid Receptor Antagonists / Q000494:pharmacology; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004409:Dyskinesia, Drug-Induced / Q000188:drug therapy* / Q000503:physiopathology; D063388:Endocannabinoids / Q000494:pharmacology*; D006220:Haloperidol / Q000009:adverse effects / Q000031:analogs & derivatives*; D008297:Male; D008409:Mastication / Q000187:drug effects / Q000502:physiology; D009068:Movement / Q000187:drug effects / Q000502:physiology; D010880:Piperidines / Q000494:pharmacology; D053284:Polyunsaturated Alkamides / Q000494:pharmacology*; D011720:Pyrazoles / Q000494:pharmacology; D017208:Rats, Wistar; D043884:Receptor, Cannabinoid, CB1 / Q000378:metabolism*; D000077285:Rimonabant; D016896:Treatment Outcome
chemical_list: D014150:Antipsychotic Agents; D001095:Arachidonic Acids; D063386:Cannabinoid Receptor Agonists; D063387:Cannabinoid Receptor Antagonists; D063388:Endocannabinoids; D010880:Piperidines; D053284:Polyunsaturated Alkamides; D011720:Pyrazoles; D043884:Receptor, Cannabinoid, CB1; C033563:haloperidol decanoate; D006220:Haloperidol; D000077285:Rimonabant; C078814:anandamide
keywords: Anandamide; Dopaminergic system; Endocannabinoids; Open field