title: Molecular and serological detection of Ehrlichia canis in naturally exposed dogs in Iran: an analysis on associated risk factors.
abstract: The general aim of this study, which was conducted for the first time in Iran, was to evaluate the seroprevalence and geographical distribution of Ehrlichia canis in a dog population in Iran, followed by molecular confirmation using PCR and sequencing. Blood samples were collected from 240 dogs in different areas of Alborz and Tehran Provinces and initially analyzed using the immunofluorescent antibody (IFA) test to detect anti-Ehrlichia canis IgG antibodies. Subsequently, nested PCR was performed based on a fragment of the 16S rRNA gene of E. canis on serologically positive samples. The results showed that 40/240 dogs (16.6%) presented anti-Ehrlichia canis IgG antibodies and that nine of the blood samples from the 40 seropositive dogs (22.5%) contained E. canis DNA, which was confirmed by sequencing. The seroprevalence of E. canis tended to be higher in purebred, one to three-year-old male dogs living in the Plain zone, in rural areas; however, this difference was not statistically significant.
authors.affiliation: Department of Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
authors.forename: Nadi
authors.initials: N
authors.lastname: Maazi
authors.affiliation: Department of Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
authors.forename: Abdolali
authors.initials: A
authors.lastname: Malmasi
authors.affiliation: Center for Ticks and Tick-borne Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
authors.forename: Parviz
authors.initials: P
authors.lastname: Shayan
authors.affiliation: Department of Pathobiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
authors.forename: Seyed Mahdi
authors.initials: SM
authors.lastname: Nassiri
authors.affiliation: Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
authors.forename: Taghi Zahraei
authors.initials: TZ
authors.lastname: Salehi
authors.affiliation: Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Belgium.
authors.forename: Mojdeh Sharifian
authors.initials: MS
authors.lastname: Fard
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D004283:Dog Diseases / Q000097:blood / Q000453:epidemiology* / Q000382:microbiology*; D004285:Dogs; D041061:Ehrlichia canis / Q000276:immunology* / Q000302:isolation & purification; D016873:Ehrlichiosis / Q000097:blood / Q000453:epidemiology / Q000662:veterinary*; D005260:Female; D007492:Iran / Q000453:epidemiology; D008297:Male; D012307:Risk Factors; D016036:Seroepidemiologic Studies
chemical_list: D000907:Antibodies, Bacterial