title: Conditions of lateral surface confinement that promote tissue-cell integration and inhibit biofilm growth.
abstract: Surfaces with cell adhesiveness modulated at micro length scales can exploit differences between tissue/bacterial cell size, membrane/wall plasticity, and adhesion mechanisms to differentially control tissue-cell/material and bacteria/material interactions. This study explores the short-term interactions of Staphylococcus aureus and osteoblast-like cells with surfaces consisting of cell-adhesive circular patches (1-5 μm diameter) separated by non-adhesive electron-beam patterned poly(ethylene glycol) hydrogel thin films at inter-patch distances of 0.5-10 μm. Osteoblast-like U2OS cells both bind to and spread on the modulated surfaces, in some cases when the cell-adhesive area comprises only 9% of the total surface and in several cases at least as well as on the continuously adhesive control surfaces. In contrast, S. aureus adhesion rates are 7-20 times less on the modulated surfaces than on the control surfaces. Furthermore, the proliferation of those bacteria that do adhere is inhibited by the lateral confinement imposed by the non-adhesive boundaries surrounding each patch. These findings suggest a new approach to create biomaterial surfaces that may promote healing while simultaneously reducing the probability of infection.
authors.affiliation: Department of Chemical Engineering and Materials Science, Stevens Institute of Technology, Hoboken, NJ, USA; University of Groningen, University Medical Center Groningen, Department of Biomedical Engineering, Antonius Deusinglaan 1, 9713 AV Groningen, The Netherlands.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.affiliation: University of Groningen, University Medical Center Groningen, Department of Biomedical Engineering, Antonius Deusinglaan 1, 9713 AV Groningen, The Netherlands.
authors.forename: Joana F
authors.initials: JF
authors.lastname: da Silva Domingues
authors.affiliation: University of Groningen, University Medical Center Groningen, Department of Biomedical Engineering, Antonius Deusinglaan 1, 9713 AV Groningen, The Netherlands.
authors.forename: Guruprakash
authors.initials: G
authors.lastname: Subbiahdoss
authors.affiliation: University of Groningen, University Medical Center Groningen, Department of Biomedical Engineering, Antonius Deusinglaan 1, 9713 AV Groningen, The Netherlands.
authors.forename: Henny C
authors.initials: HC
authors.lastname: van der Mei
authors.affiliation: University of Groningen, University Medical Center Groningen, Department of Biomedical Engineering, Antonius Deusinglaan 1, 9713 AV Groningen, The Netherlands.
authors.forename: Henk J
authors.initials: HJ
authors.lastname: Busscher
authors.affiliation: Department of Chemical Engineering and Materials Science, Stevens Institute of Technology, Hoboken, NJ, USA. Electronic address: mlibera@stevens.edu.
authors.forename: Matthew
authors.initials: M
authors.lastname: Libera
mesh_terms: D001422:Bacterial Adhesion; D001672:Biocompatible Materials / Q000737:chemistry*; D018441:Biofilms / Q000254:growth & development*; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D006801:Humans; D020100:Hydrogels / Q000737:chemistry; D010006:Osteoblasts / Q000378:metabolism / Q000382:microbiology; D011092:Polyethylene Glycols / Q000737:chemistry; D013211:Staphylococcus aureus / Q000254:growth & development; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D020100:Hydrogels; D011092:Polyethylene Glycols
keywords: Biofilm; Cell adhesion; Infection; Micropatterning; Osteoblast; Polyethylene oxide