title: Spirosoma radiotolerans sp. nov., a gamma-radiation-resistant bacterium isolated from gamma ray-irradiated soil.
abstract: A Gram-negative, short-rod-shaped bacterial strain with gliding motility, designated as DG5A(T), was isolated from a rice field soil in South Korea. Phylogenic analysis using 16S rRNA gene sequence of the new isolate showed that strain DG5A(T) belong to the genus Spirosoma in the family Spirosomaceae, and the highest sequence similarities were 95.5 % with Spirosoma linguale DSM 74(T), 93.4 % with Spirosoma rigui WPCB118(T), 92.8 % with Spirosoma luteum SPM-10(T), 92.7 % with Spirosoma spitsbergense SPM-9(T), and 91.9 % with Spirosoma panaciterrae Gsoil 1519(T). Strain DG5A(T) revealed resistance to gamma and UV radiation. Chemotaxonomic data showed that the most abundant fatty acids were summed feature C(16:1) ω7c/C(16:1) ω6c (36.90 %), C(16:1) ω5c (29.55 %), and iso-C(15:0) (14.78 %), and the major polar lipid was phosphatidylethanolamine (PE). The DNA G+C content of strain DG5A(T) was 49.1 mol%. Together, the phenotypic, phylogenetic, and chemotaxonomic data supported that strain DG5A(T) presents a novel species of the genus Spirosoma, for which the name Spirosoma radiotolerans sp. nov., is proposed. The type strain is DG5A(T) (=KCTC 32455(T) = JCM19447(T)).
authors.affiliation: Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University, Seoul, 139-774, Republic of Korea.
authors.forename: Jae-Jin
authors.initials: JJ
authors.lastname: Lee
authors.forename: Sathiyaraj
authors.initials: S
authors.lastname: Srinivasan
authors.forename: Sangyong
authors.initials: S
authors.lastname: Lim
authors.forename: Minho
authors.initials: M
authors.lastname: Joe
authors.forename: Seonghun
authors.initials: S
authors.lastname: Im
authors.forename: So Il
authors.initials: SI
authors.lastname: Bae
authors.forename: Kyoung Ryun
authors.initials: KR
authors.lastname: Park
authors.forename: Ji-Hee
authors.initials: JH
authors.lastname: Han
authors.forename: Se-Hee
authors.initials: SH
authors.lastname: Park
authors.forename: Bo-Min
authors.initials: BM
authors.lastname: Joo
authors.forename: Sol-Ji
authors.initials: SJ
authors.lastname: Park
authors.forename: Myung Kyum
authors.initials: MK
authors.lastname: Kim
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D016000:Cluster Analysis; D041964:Cytophagaceae / Q000145:classification* / Q000302:isolation & purification* / Q000502:physiology / Q000528:radiation effects; D003600:Cytosol / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005720:Gamma Rays; D007723:Korea; D008124:Locomotion; D050296:Microbial Viability / Q000528:radiation effects*; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D012275:Oryza; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D014466:Ultraviolet Rays
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S
references.citation: Appl Environ Microbiol. 2005 Sep;71(9):5225-35
references.pmid: 16151108
references.citation: Bioprocess Biosyst Eng. 2013 Jun;36(6):781-9
references.pmid: 23355081
references.citation: J Bacteriol. 1991 Jan;173(2):697-703
references.pmid: 1987160
references.citation: Mol Biol Evol. 1987 Jul;4(4):406-25
references.pmid: 3447015
references.citation: Mol Biol Evol. 2011 Oct;28(10):2731-9
references.pmid: 21546353
references.citation: Int J Syst Evol Microbiol. 2009 Feb;59(Pt 2):331-5
references.pmid: 19196773
references.citation: Microbiol Rev. 1981 Jun;45(2):316-54
references.pmid: 7022156
references.citation: Evolution. 1985 Jul;39(4):783-791
references.pmid: 28561359
references.citation: Int J Syst Evol Microbiol. 2013 Feb;63(Pt 2):696-702
references.pmid: 22544798
references.citation: Nucleic Acids Res. 1997 Dec 15;25(24):4876-82
references.pmid: 9396791
references.citation: Int J Syst Evol Microbiol. 2007 Dec;57(Pt 12):2870-2873
references.pmid: 18048741
references.citation: Int J Syst Evol Microbiol. 2012 Mar;62(Pt 3):716-721
references.pmid: 22140171
references.citation: Int J Syst Evol Microbiol. 2011 Jun;61(Pt 6):1418-1424
references.pmid: 20601484
references.citation: J Mol Evol. 1980 Dec;16(2):111-20
references.pmid: 7463489
references.citation: Int J Syst Bacteriol. 1998 Jan;48 Pt 1:223-35
references.pmid: 9542092
references.citation: Int J Syst Evol Microbiol. 2008 Feb;58(Pt 2):474-8
references.pmid: 18218952
references.citation: Appl Environ Microbiol. 1982 Oct;44(4):992-3
references.pmid: 6184019
references.citation: PLoS One. 2013 Jul 01;8(7):e69007
references.pmid: 23840905
references.citation: Trends Microbiol. 1999 Sep;7(9):362-5
references.pmid: 10470044
references.citation: Int J Syst Evol Microbiol. 2009 Apr;59(Pt 4):839-44
references.pmid: 19329617