title: Stationary phases for the enrichment of glycoproteins and glycopeptides.
abstract: The analysis of protein glycosylation is important for biomedical and biopharmaceutical research. Recent advances in LC-MS analysis have enabled the identification of glycosylation sites, the characterisation of glycan structures and the identification and quantification of glycoproteins and glycopeptides. However, this type of analysis remains challenging due to the low abundance of glycopeptides in complex protein digests, the microheterogeneity at glycosylation sites, ion suppression effects and the competition for ionisation by co-eluting peptides. Specific sample preparation is necessary for comprehensive and site-specific glycosylation analyses using MS. Therefore, researchers continue to pursue new columns to broaden their applications. The current manuscript covers recent literature published from 2008 to 2013. The stationary phases containing various chemical bonding methods or ligands immobilisation strategies on solid supports that selectively enrich N-linked or sialylated N-glycopeptides are categorised with either physical or chemical modes of binding. These categories include lectin affinity, hydrophilic interactions, boronate affinity, titanium dioxide affinity, hydrazide chemistry and other separation techniques. This review should aid in better understanding the syntheses and physicochemical properties of each type of stationary phases for enriching glycoproteins and glycopeptides.
authors.affiliation: Department of Chemistry, National Taiwan University, Taipei, Taiwan.
authors.forename: Bao-Yu
authors.initials: BY
authors.lastname: Huang
authors.forename: Chun-Kai
authors.initials: CK
authors.lastname: Yang
authors.forename: Ching-Piao
authors.initials: CP
authors.lastname: Liu
authors.forename: Chuen-Ying
authors.initials: CY
authors.lastname: Liu
mesh_terms: D000818:Animals; D001897:Boronic Acids; D002845:Chromatography / Q000295:instrumentation / Q000379:methods; D057930:Click Chemistry / Q000295:instrumentation / Q000379:methods; D006020:Glycopeptides / Q000032:analysis / Q000302:isolation & purification; D006023:Glycoproteins / Q000032:analysis / Q000302:isolation & purification; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D037102:Lectins; D051379:Mice
chemical_list: D001897:Boronic Acids; D006020:Glycopeptides; D006023:Glycoproteins; D037102:Lectins
keywords: Boronate; Click chemistry; Hydrazide; Hydrophilic interaction; Lectin