title: Biocompatibility of Portland cement combined with different radiopacifying agents.
abstract: The aim of this study was to evaluate the response of rat subcutaneous tissue to Portland cement combined with two different radiopacifying agents, iodoform (CHI3) and zirconium oxide (ZrO2). These materials were placed in polyethylene tubes and implanted into the dorsal connective tissue of Wistar rats for 7 and 15 days. The specimens were then stained with hematoxylin and eosin, and inflammatory reaction parameters were evaluated by light microscopy. The intensity of the inflammatory response to the sealants was analyzed by two blind calibrated observers throughout the experimental period. Histological analysis showed that all the materials caused a moderated inflammatory reaction at 7 days, which then diminished with time. At 15 days, the inflammatory reaction was almost absent, and fibroblasts and collagen fibers were observed indicating normal tissue healing. The degrees of the inflammatory reaction on different days throughout the experimental period were compared using the non-parametric Kruskal-Wallis test. Statistical analysis demonstrated no significant differences amongst the groups, and Portland cement associated with radiopacifying agents gave satisfactory results. Therefore, Portland cement used in combination with radiopacifying agents can be considered a biocompatible material. Although our results are very encouraging, further studies are needed in order to establish safe clinical indications for Portland cement combined with radiopacifying agents.
authors.affiliation: Department of Pediatric Dentistry, Orthodontics and Public Health, Bauru School of Dentistry, University of São Paulo.
authors.forename: Natalino
authors.initials: N
authors.lastname: Lourenço Neto
authors.forename: Nádia C T
authors.initials: NC
authors.lastname: Marques
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: Fernandes
authors.forename: Camila O
authors.initials: CO
authors.lastname: Rodini
authors.forename: Marco A H
authors.initials: MA
authors.lastname: Duarte
authors.forename: Marta C
authors.initials: MC
authors.lastname: Lima
authors.forename: Maria A A M
authors.initials: MA
authors.lastname: Machado
authors.forename: Ruy C C
authors.initials: RC
authors.lastname: Abdo
authors.forename: Thais M
authors.initials: TM
authors.lastname: Oliveira
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D003738:Dental Cements; D008297:Male; D051381:Rats; D017208:Rats, Wistar
chemical_list: D001672:Biocompatible Materials; D003738:Dental Cements