title: Histidine-mediated xylem loading of zinc is a species-wide character in Noccaea caerulescens.
abstract: Histidine plays a crucial role in nickel (Ni) translocation in Ni-hyperaccumulating plants. Here, we investigated its role in zinc (Zn) translocation in four accessions of the Zn hyperaccumulator, Noccaea caerulescens, using the related non-hyperaccumulator, Thlaspi arvense, as a reference. We compared the effects of exogenous histidine supply on Zn xylem loading, and of Zn-histidine complex formation on Zn uptake in energized tonoplast vesicles. The Zn distribution patterns over root tissues were also compared. Exogenous histidine supply enhanced Zn xylem loading in all the N. caerulescens accessions, but decreased it in T. arvense. Zn distribution patterns over root tissues were similar, apart from the accumulation in cortical and endodermal cells, which was much lower in N. caerulescens than in T. arvense. Zn uptake in energized tonoplast vesicles was inhibited significantly in N. caerulescens, but not affected significantly in T. arvense, when Zn was supplied in combination with histidine in a 1:2 molar ratio. Histidine-mediated Zn xylem loading seems to be a species-wide character in N. caerulescens. It may well have evolved as a component trait of the hyperaccumulation machinery for Zn, rather than for Ni.
authors.affiliation: Laboratory of Root Physiology, Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya str. 35, 127276, Moscow, Russia.
authors.forename: Anna D
authors.initials: AD
authors.lastname: Kozhevnikova
authors.affiliation: Laboratory of Root Physiology, Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya str. 35, 127276, Moscow, Russia.
authors.forename: Ilya V
authors.initials: IV
authors.lastname: Seregin
authors.affiliation: Laboratory of Root Physiology, Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya str. 35, 127276, Moscow, Russia.
authors.forename: Nadezhda T
authors.initials: NT
authors.lastname: Erlikh
authors.affiliation: Laboratory of Plant Cell Membranes, Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya str. 35, 127276, Moscow, Russia.
authors.forename: Taisiya A
authors.initials: TA
authors.lastname: Shevyreva
authors.affiliation: Laboratory of Plant Cell Membranes, Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya str. 35, 127276, Moscow, Russia.
authors.forename: Igor M
authors.initials: IM
authors.lastname: Andreev
authors.affiliation: Department of Animal Ecology, Faculty of Earth and Life Sciences, Vrije Universiteit Amsterdam, de Boelelaan 1085, 1081 HV, Amsterdam, the Netherlands.
authors.forename: Rudo
authors.initials: R
authors.lastname: Verweij
authors.affiliation: Department of Genetics, Faculty of Earth and Life Sciences, Vrije Universiteit Amsterdam, de Boelelaan 1085, 1081 HV, Amsterdam, the Netherlands.
authors.forename: Henk
authors.initials: H
authors.lastname: Schat
mesh_terms: D019607:Brassicaceae / Q000187:drug effects / Q000378:metabolism*; D006639:Histidine; D017136:Ion Transport; D009942:Organometallic Compounds; D018515:Plant Leaves / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism; D013045:Species Specificity; D031227:Thlaspi / Q000187:drug effects / Q000378:metabolism; D014018:Tissue Distribution; D052584:Xylem / Q000378:metabolism*; D015032:Zinc / Q000378:metabolism / Q000493:pharmacokinetics*
chemical_list: D009942:Organometallic Compounds; C052388:zinc-histidine complex; D006639:Histidine; D015032:Zinc
keywords: Noccaea caerulescens; Thlaspi arvense; histidine; hyperaccumulation; tissue distribution; xylem loading; zinc (Zn)
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