title: Circulating cell-free DNA in cancer.
abstract: This papers deals with the preanalytical and analytical phase of cell-free DNA analysis, highlighting some criticism on sample collection and extraction. We describe a method to accurately quantify total cfDNA in plasma and our particular approach to the measurement of tumor deriving cfDNA.
authors.affiliation: Department of Biomedical, Experimental and Clinical Sciences, University of Florence, Viale Pieraccini 6, Florence 50139, Italy, p.pinzani@dfc.unifi.it.
authors.forename: Pamela
authors.initials: P
authors.lastname: Pinzani
authors.forename: Francesca
authors.initials: F
authors.lastname: Salvianti
authors.forename: Claudio
authors.initials: C
authors.lastname: Orlando
authors.forename: Mario
authors.initials: M
authors.lastname: Pazzagli
mesh_terms: D000483:Alleles; D001800:Blood Specimen Collection; D002474:Cell-Free System / Q000378:metabolism; D004273:DNA, Neoplasm / Q000097:blood* / Q000235:genetics; D006801:Humans; D009154:Mutation; D009369:Neoplasms / Q000097:blood*; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D004273:DNA, Neoplasm