title: Expression of Niban in renal interstitial fibrosis.
abstract: OBJECTIVE
  Apoptosis is one of the most important mechanisms underlying renal interstitial fibrosis. We identified the role of protein Niban in apoptosis of tumour cells. The purpose of this study was to assess the expression of Niban in renal interstitial fibrosis of humans and rats.
  
  
  METHODS
  Immunohistochemistry was used to detect Niban in patients with obstructive nephropathy. Proteomics and gene array analysis were performed to screen different molecules involved in the pathophysiology of unilateral-ureteral obstruction rats. We confirmed Niban using immunohistochemistry and Western blot in renal cortex of UUO rats and HK-2 cells. TUNEL assay and flow cytometry revealed apoptosis of renal tubular cells. siRNA and overexpression plasmid were transfected specifically to study the possible function of Niban.
  
  
  RESULTS
  Niban was decreased apparently in renal tubular cells of patients with obstructive nephropathy, compared with controls. Niban decreased in renal cortex of UUO rats and transforming growth factor-β1 (TGF-β1)-stimulated HK-2 cells. siRNA of Niban increased apoptosis of HK-2 cells. TGF-β1 also increased apoptosis of HK-2 cells. Overexpression of Niban failed to diminish apoptosis of HK-2 cells induced by TGF-β1.
  
  
  CONCLUSIONS
  Niban decreased in renal tubular cells of patients of obstructive nephropathy, UUO rats and TGF-β1 stimulated HK-2 cells. Suppressing Niban increases apoptosis in HK-2 cells. Niban may be associated with apoptosis of HK-2 cells.
authors.affiliation: Division of Nephrology, Xiangya Hospital, Central South University, Changsha, China.
authors.forename: Jishi
authors.initials: J
authors.lastname: Liu
authors.forename: Jiao
authors.initials: J
authors.lastname: Qin
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Mei
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.forename: Qiongjing
authors.initials: Q
authors.lastname: Yuan
authors.forename: Zhangzhe
authors.initials: Z
authors.lastname: Peng
authors.forename: Renna
authors.initials: R
authors.lastname: Luo
authors.forename: Xiangning
authors.initials: X
authors.lastname: Yuan
authors.forename: Ling
authors.initials: L
authors.lastname: Huang
authors.forename: Lijian
authors.initials: L
authors.lastname: Tao
mesh_terms: D000818:Animals; D017209:Apoptosis; D014408:Biomarkers, Tumor / Q000032:analysis / Q000096:biosynthesis*; D002478:Cells, Cultured; D005355:Fibrosis / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007668:Kidney / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology*; D008297:Male; D009363:Neoplasm Proteins / Q000032:analysis / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D051017:Apoptosis Regulatory Proteins
chemical_list: D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; C516108:NIBAN1 protein, human; D051017:Apoptosis Regulatory Proteins
keywords: Chronic Kidney Disease; Niban (Fam129a); apoptosis; renal interstitial fibrosis