title: Possible effects of nitric oxide synthases on odor-induced behavioral changes in mice.
abstract: Although exposure to olfactory stimulants can induce neurobehavioral changes, there is a lack of data regarding specific effects on neurotransduction, especially nitric oxide (NO)-mediated neurotransduction. We investigated the relationship between altered behavioral performance and changes in the expression of nitric oxide synthase (NOS), nNOS, iNOS, and eNOS, in 2-methylbutyric (2MB) acid-exposed mice. Mice were exposed to 2MB acid by inhalation and olfactory preference/avoidance and motor coordination were measured. Additionally, we examined NOS expression in the olfactory bulb of the mouse brain. Mice exposed to 2MB acid showed significant changes in olfactory preference and rotarod activity as compared with controls. Although there was no change in nNOS and iNOS expressions in the olfactory bulb of 2MB acid-exposed mice, eNOS expression increased significantly in the olfactory bulb of 9.0M 2MB acid-exposed mice. These data indicate that altered eNOS expression in the olfactory bulb may contribute to 2MB acid-induced behavioral changes.
authors.affiliation: College of Pharmacy, Keimyung University, Daegu 704-701, Republic of Korea.
authors.forename: Kyunghwa
authors.initials: K
authors.lastname: Sung
authors.affiliation: College of Pharmacy, Keimyung University, Daegu 704-701, Republic of Korea; College of Pharmacy, Ewha Womans University, Seoul 151-742, Republic of Korea.
authors.forename: Minjeong
authors.initials: M
authors.lastname: Kim
authors.affiliation: Department of Public Health, Keimyung University, Daegu 704-701, Republic of Korea.
authors.forename: Jiyoung
authors.initials: J
authors.lastname: Hyun
authors.affiliation: Department of Skin and Health Management, Suseong College, Daegu 706-711, Republic of Korea.
authors.forename: Younghee
authors.initials: Y
authors.lastname: Kim
authors.affiliation: College of Pharmacy, Keimyung University, Daegu 704-701, Republic of Korea. Electronic address: kimkisok@kmu.ac.kr.
authors.forename: Kisok
authors.initials: K
authors.lastname: Kim
mesh_terms: D000818:Animals; D001362:Avoidance Learning; D001522:Behavior, Animal; D002087:Butyrates / Q000494:pharmacology; D008297:Male; D008810:Mice, Inbred C57BL; D009043:Motor Activity; D009569:Nitric Oxide / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D052248:Nitric Oxide Synthase Type I / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism; D009812:Odorants; D009830:Olfactory Bulb / Q000378:metabolism; D045442:Rotarod Performance Test
chemical_list: D002087:Butyrates; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D052248:Nitric Oxide Synthase Type I; D052247:Nitric Oxide Synthase Type II; D052250:Nitric Oxide Synthase Type III; C496321:Nos1 protein, mouse; C496317:Nos2 protein, mouse; C496315:Nos3 protein, mouse; C019475:2-methylbutanoic acid
keywords: 2-Methylbutyric acid; Behavioral change; Nitric oxide synthase; Olfactory bulb