title: Phanerochaete chrysosporium inoculation shapes the indigenous fungal communities during agricultural waste composting.
abstract: Inoculation with exogenous white-rot fungi has been proven to be an efficient method to promote lignocellulose biodegradation during agricultural waste composting. Indigenous fungal communities, the most important organisms responsible for mineralization and decomposition of lignocellulosic materials in composts, can be affected by sample properties and other biotic factors. This research was conducted to determine the effects of the Phanerochaete chrysosporium inoculation on the indigenous fungal communities during agricultural waste composting. Fungal communities in samples with different inoculation regimes were investigated by sequencing and quantitative PCR. Results showed that P. chrysosporium inoculants produced significant negative effects on the indigenous fungal community abundance during the thermophilic stage. Samples inoculated during Phase II contained higher proportion of Acremonium chrysogenum and Galactomyces geotrichum, while those non-inoculated samples were dominated by Coprinopsis cinerea and Scytalidium thermophilum. Moreover, the indigenous fungal community abundance was significantly correlated with the C/N ratio, water soluble carbon and moisture content (P < 0.05). Redundancy analysis indicated that the most variation in distribution of indigenous fungal community structure was statistically explained by nitrate, C/N ratio, and moisture content, factors which solely explained 29.6 % (F = 30.316, P = 0.002), 25.6 % (F = 26.191, P = 0.002) and 10.0 % (F = 10.249, P = 0.002) of the variation in the indigenous fungal community structure, respectively.
authors.affiliation: College of Resources and Environment, Hunan Agricultural University, Changsha, 410128, China.
authors.forename: Jiachao
authors.initials: J
authors.lastname: Zhang
authors.forename: Guangming
authors.initials: G
authors.lastname: Zeng
authors.forename: Yaoning
authors.initials: Y
authors.lastname: Chen
authors.forename: Jie
authors.initials: J
authors.lastname: Liang
authors.forename: Chang
authors.initials: C
authors.lastname: Zhang
authors.forename: Binbin
authors.initials: B
authors.lastname: Huang
authors.forename: Weimin
authors.initials: W
authors.lastname: Sun
authors.forename: Ming
authors.initials: M
authors.lastname: Chen
authors.forename: Man
authors.initials: M
authors.lastname: Yu
authors.forename: Hongli
authors.initials: H
authors.lastname: Huang
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhu
mesh_terms: D000383:Agriculture; D001673:Biodegradation, Environmental; D020075:Phanerochaete / Q000378:metabolism*; D012988:Soil Microbiology