title: A biocatalytic cascade with several output signals--towards biosensors with different levels of confidence.
abstract: The biocatalytic cascade based on enzyme-catalyzed reactions activated by several biomolecular input signals and producing output signal after each reaction step was developed as an example of a logically reversible information processing system. The model system was designed to mimic the operation of concatenated AND logic gates with optically readable output signals generated at each step of the logic operation. Implications include concurrent bioanalyses and data interpretation for medical diagnostics.
authors.affiliation: Department of Chemistry and Biomolecular Science, Clarkson University, Potsdam, NY, 13699-5810, USA.
authors.forename: Nataliia
authors.initials: N
authors.lastname: Guz
authors.forename: Jan
authors.initials: J
authors.lastname: Halámek
authors.forename: James F
authors.initials: JF
authors.lastname: Rusling
authors.forename: Evgeny
authors.initials: E
authors.lastname: Katz
mesh_terms: D000244:Adenosine Diphosphate / Q000737:chemistry; D000255:Adenosine Triphosphate / Q000737:chemistry; D001681:Biological Assay / Q000295:instrumentation* / Q000379:methods; D015415:Biomarkers / Q000737:chemistry; D015374:Biosensing Techniques; D002384:Catalysis; D002623:Chemistry Techniques, Analytical; D003955:Diagnostic Tests, Routine; D005988:Glyceric Acids / Q000737:chemistry; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000737:chemistry; D006899:Mixed Function Oxygenases / Q000737:chemistry; D009243:NAD / Q000737:chemistry; D055095:Optics and Photonics; D010100:Oxygen / Q000737:chemistry; D010728:Phosphoenolpyruvate / Q000737:chemistry; D010751:Phosphopyruvate Hydratase / Q000737:chemistry
chemical_list: D015415:Biomarkers; D005988:Glyceric Acids; D009243:NAD; C008885:2-phosphoglycerate; D000244:Adenosine Diphosphate; D010728:Phosphoenolpyruvate; D000255:Adenosine Triphosphate; D006899:Mixed Function Oxygenases; D007770:L-Lactate Dehydrogenase; C021497:lactate 2-monooxygenase; D010751:Phosphopyruvate Hydratase; D010100:Oxygen
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