title: Identification of B-cell lymphoma subsets by plasma protein profiling using recombinant antibody microarrays.
abstract: B-cell lymphoma (BCL) heterogeneity represents a key issue, often making the classification and clinical management of these patients challenging. In this pilot study, we outlined the first resolved view of BCL disease heterogeneity on the protein level by deciphering disease-associated plasma biomarkers, specific for chronic lymphocytic leukemia, diffuse large B-cell lymphoma, follicular lymphoma, and mantle cell lymphoma, using recombinant antibody microarrays targeting mainly immunoregulatory proteins. The results showed the BCLs to be heterogeneous, and revealed potential novel subgroups of each BCL. In the case of diffuse large B-cell lymphoma, we also indicated a link between the novel subgroups and survival.
authors.affiliation: Department of Immunotechnology, Lund University, Lund, Sweden; CREATE Health, Lund University, Lund, Sweden.
authors.forename: Frida
authors.initials: F
authors.lastname: Pauly
authors.affiliation: Department of Medicine Solna, Clinical Epidemiology Unit, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Karin E
authors.initials: KE
authors.lastname: Smedby
authors.affiliation: Department of Oncology, Skåne University Hospital, Lund, Sweden.
authors.forename: Mats
authors.initials: M
authors.lastname: Jerkeman
authors.affiliation: Department of Epidemiology Research, Statens Serum Institute, Copenhagen, Denmark.
authors.forename: Henrik
authors.initials: H
authors.lastname: Hjalgrim
authors.affiliation: Computational Biology & Biological Physics, Department of Astronomy and Theoretical Physics, Lund University, Lund, Sweden; CREATE Health, Lund University, Lund, Sweden.
authors.forename: Mattias
authors.initials: M
authors.lastname: Ohlsson
authors.affiliation: Department of Immunology, Genetics and Pathology, Science for Life Laboratory, Uppsala University, Uppsala, Sweden.
authors.forename: Richard
authors.initials: R
authors.lastname: Rosenquist
authors.affiliation: Department of Immunotechnology, Lund University, Lund, Sweden; CREATE Health, Lund University, Lund, Sweden.
authors.forename: Carl A K
authors.initials: CA
authors.lastname: Borrebaeck
authors.affiliation: Department of Immunotechnology, Lund University, Lund, Sweden; CREATE Health, Lund University, Lund, Sweden. Electronic address: christer.wingren@immun.lth.se.
authors.forename: Christer
authors.initials: C
authors.lastname: Wingren
mesh_terms: D000368:Aged; D000906:Antibodies / Q000276:immunology; D001798:Blood Proteins / Q000032:analysis*; D005260:Female; D006801:Humans; D016393:Lymphoma, B-Cell / Q000097:blood / Q000145:classification*; D008297:Male; D008875:Middle Aged; D010865:Pilot Projects; D040081:Protein Array Analysis / Q000379:methods*; D020543:Proteome; D011994:Recombinant Proteins / Q000276:immunology
chemical_list: D000906:Antibodies; D001798:Blood Proteins; D020543:Proteome; D011994:Recombinant Proteins
keywords: B-cell lymphoma; Biomarker; CLL; DLBCL; Disease heterogeneity; FL; MCL; Plasma protein profiling