title: Parahydrogen induced polarization of 1-(13)C-phospholactate-d(2) for biomedical imaging with >30,000,000-fold NMR signal enhancement in water.
abstract: The synthetic protocol for preparation of 1-(13)C-phosphoenolpyruvate-d2, precursor for parahydrogen-induced polarization (PHIP) of 1-(13)C-phospholactate-d2, is reported. (13)C nuclear spin polarization of 1-(13)C-phospholactate-d2 was increased by >30,000,000-fold (5.75 mT) in water. The reported (13)C polarization level approaching unity (>15.6%), long lifetime of (13)C hyperpolarized 1-(13)C-phospholactate-d2 (58 ± 4 s versus 36 ± 2 s for nondeuterated form at 47.5 mT), and large production quantities (52 μmoles in 3 mL) in aqueous medium make this compound useful as a potential contrast agent for the molecular imaging of metabolism and other applications.
authors.affiliation: Vanderbilt University Institute of Imaging Science (VUIIS), Department of Radiology, ‡Department of Biomedical Engineering and Biochemistry, §Department of Physics and Astronomy, ∥Department of Biochemistry, ¶Vanderbilt-Ingram Cancer Center (VICC), Vanderbilt University , Nashville, Tennessee 37232, United States.
authors.forename: Roman V
authors.initials: RV
authors.lastname: Shchepin
authors.forename: Aaron M
authors.initials: AM
authors.lastname: Coffey
authors.forename: Kevin W
authors.initials: KW
authors.lastname: Waddell
authors.forename: Eduard Y
authors.initials: EY
authors.lastname: Chekmenev
mesh_terms: D006859:Hydrogen / Q000737:chemistry*; D007773:Lactates / Q000737:chemistry*; D066244:Proton Magnetic Resonance Spectroscopy / Q000379:methods*; D014867:Water / Q000737:chemistry*
chemical_list: D007773:Lactates; D014867:Water; D006859:Hydrogen
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