title: Silymarin induces expression of pancreatic Nkx6.1 transcription factor and β-cells neogenesis in a pancreatectomy model.
abstract: A physio-pathological feature of diabetes mellitus is a significant reduction of β-pancreatic cells. The growth, differentiation and function maintenance of these cells is directed by transcription factors. Nkx6.1 is a key transcription factor for the differentiation, neogenesis and maintenance of β-pancreatic cells. We reported that silymarin restores normal morphology and endocrine function of damaged pancreatic tissue after alloxan-induced diabetes mellitus in rats. The aim of this study was to analyze the effect of silymarin on Nkx6.1 transcription factor expression and its consequence in β cells neogenesis. Sixty male Wistar rats were partially pancreatectomized and divided into twelve groups. Six groups were treated with silymarin (200 mg/Kg p.o) for periods of 3, 7, 14, 21, 42 and 63 days. Additionally, an unpancreatectomized control group was used. Nkx6.1 and insulin gene expression were assessed by RT-PCR assay in total pancreatic RNA. β-Cell neogenesis was determined by immunoperoxidase assay. Silymarin treated group showed an increase of Nkx6.1 and insulin genic expression. In this group, there was an increment of β-cell neogenesis in comparison to pancreatectomized untreated group. Silymarin treatment produced a rise in serum insulin and serum glucose normalization. These results suggest that silymarin may improve the reduction of β pancreatic cells observed in diabetes mellitus.
authors.affiliation: Departamento de Sistemas Biológicos, Universidad Autónoma Metropolitana-Xochimilco, México City 04960, Mexico. casoto@correo.xoc.uam.mx.
authors.forename: Claudia
authors.initials: C
authors.lastname: Soto
authors.affiliation: Facultad de Medicina, Universidad Nacional Autónoma de México, México City 04510, Mexico. andres.2508@hotmail.com.
authors.forename: Luis
authors.initials: L
authors.lastname: Raya
authors.affiliation: Departamento de Sistemas Biológicos, Universidad Autónoma Metropolitana-Xochimilco, México City 04960, Mexico. jperez@correo.xoc.uam.mx.
authors.forename: Julia
authors.initials: J
authors.lastname: Pérez
authors.affiliation: Departamento de Sistemas Biológicos, Universidad Autónoma Metropolitana-Xochimilco, México City 04960, Mexico. ha_des_dark@hotmail.com.
authors.forename: Imelda
authors.initials: I
authors.lastname: González
authors.affiliation: Departamento de Sistemas Biológicos, Universidad Autónoma Metropolitana-Xochimilco, México City 04960, Mexico. msperez@correo.xoc.uam.mx.
authors.forename: Salud
authors.initials: S
authors.lastname: Pérez
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D002452:Cell Count; D049109:Cell Proliferation; D003920:Diabetes Mellitus; D004195:Disease Models, Animal; D015870:Gene Expression; D018398:Homeodomain Proteins / Q000819:agonists* / Q000235:genetics / Q000378:metabolism; D007124:Immunoenzyme Techniques; D007328:Insulin / Q000819:agonists* / Q000235:genetics / Q000378:metabolism; D050417:Insulin-Secreting Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D010180:Pancreatectomy; D020011:Protective Agents / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012838:Silymarin / Q000494:pharmacology*
chemical_list: D001786:Blood Glucose; D018398:Homeodomain Proteins; D007328:Insulin; C491327:Nkx6-1 protein, rat; D020011:Protective Agents; D012838:Silymarin
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