title: Predictive modeling for growth of non- and cold-adapted Listeria monocytogenes on fresh-cut cantaloupe at different storage temperatures.
abstract: The aim of this study was to determine the growth kinetics of Listeria monocytogenes, with and without cold-adaption, on fresh-cut cantaloupe under different storage temperatures. Fresh-cut samples, spot inoculated with a 4-strain cocktail of L. monocytogenes (∼3.2 log CFU/g), were exposed to constant storage temperatures held at 10, 15, 20, 25, or 30 °C. All growth curves of L. monocytogenes were fitted to the Baranyi, modified Gompertz, and Huang models. Regardless of conditions under which cells grew, the time needed to reach 5 log CFU/g decreased with the elevated storage temperature. Experimental results showed that there were no significant differences (P > 0.05) in the maximum growth rate k (log CFU/g h(-1) ) and lag phase duration λ (h) between the cultures of L. monocytogenes with or without previous cold-adaption treatments. No distinct difference was observed in the growth pattern among 3 primary models at various storage temperatures. The growth curves of secondary modeling were fitted on an Arrhenius-type model for describing the relationship between k and temperature of the L. monocytogenes on fresh-cut cantaloupe from 10 to 30 °C. The root mean square error values of secondary models for non- and cold-adapted cells were 0.018, 0.021, and 0.024, and 0.039, 0.026, and 0.017 at the modified Gompertz, Baranyi, and Huang model, respectively, indicating that these 3 models presented the good statistical fit. This study may provide valuable information to predict the growth of L. monocytogenes on fresh-cut cantaloupes at different storage conditions.
  
  
  CONCLUSIONS
  Listeriosis has occurred and increased along with the increased demand of fresh and fresh-cut fruits and vegetables. This study was conducted to predict the growth of non- and cold-adapted L. monocytogenes on fresh-cut cantaloupe at different temperature using mathematical model. These results can be helpful for risk assessments of L. monocytogenes in fresh-cut cantaloupe. This study provides valuable information to food handlers to choose proper storage temperatures for extending the shelf-life of fresh-cut cantaloupe.
authors.affiliation: Dept. of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon Natl. Univ., Chuncheon, Gangwondo, 200-701, South Korea.
authors.forename: Yoon-Ki
authors.initials: YK
authors.lastname: Hong
authors.forename: Won Byong
authors.initials: WB
authors.lastname: Yoon
authors.forename: Lihan
authors.initials: L
authors.lastname: Huang
authors.forename: Hyun-Gyun
authors.initials: HG
authors.lastname: Yuk
mesh_terms: D000222:Adaptation, Physiological; D003080:Cold Temperature; D015169:Colony Count, Microbial; D003257:Consumer Product Safety; D028463:Cucumis melo / Q000382:microbiology*; D005511:Food Handling / Q000379:methods; D005516:Food Microbiology; D005638:Fruit / Q000382:microbiology*; D006801:Humans; D007700:Kinetics; D008089:Listeria monocytogenes / Q000254:growth & development*; D008954:Models, Biological; D013696:Temperature
keywords: Listeria monocytogenes; cold-adaptation; fresh-cut cantaloupe; predictive modeling