title: Enhanced production of ATP-binding cassette protein exporter-dependent lipase by modifying the growth medium components of Pseudomonas fluorescens.
abstract: The industrially-important thermostable lipase, TliA, was extracellularly produced in the recombinant Pseudomonas fluorescens by the homologous expression of TliA and its cognate ABC protein exporter, TliDEF. To increase the secretory production of TliA, we optimized the growth temperature and the culture medium of P. fluorescens. The total amount and the specific productivity of lipase was highest at 25 °C of cell growth temperature, although maximal cell growth was observed at 30 °C. Using the culture medium composed of 20 g dextrin l(-1), 40 g Tween 80 l(-1) and 30 g peptone l(-1), TliA was produced at a level of 2,200 U ml(-1) in a flask culture. The TliA production increased about 3.8-fold (8,450 U ml(-1)) in batch fermentation using a 2.5 l fermentor, which was about 7.7-fold higher than that of previously reported TliA production.
authors.affiliation: Research Center for Bio-based Chemistry, Korea Research Institute of Chemical Technology (KRICT), 141 Gajeong-ro, Yuseong-gu, Daejeon, 305-600, Republic of Korea.
authors.forename: Gyeong Tae
authors.initials: GT
authors.lastname: Eom
authors.forename: Jae Kwang
authors.initials: JK
authors.lastname: Song
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000378:metabolism*; D001426:Bacterial Proteins; D061249:Batch Cell Culture Techniques; D019149:Bioreactors / Q000382:microbiology; D002244:Carbon / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D003470:Culture Media / Q000737:chemistry*; D005285:Fermentation / Q000187:drug effects; D008049:Lipase / Q000096:biosynthesis*; D009584:Nitrogen / Q000494:pharmacology; D011551:Pseudomonas fluorescens / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism*; D013696:Temperature
chemical_list: D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; D003470:Culture Media; D002244:Carbon; D008049:Lipase; C428386:thermostable lipase; D009584:Nitrogen