title: Growth inhibitory, bactericidal, and morphostructural effects of dehydrocostus lactone from Magnolia sieboldii Leaves on antibiotic-susceptible and -resistant strains of Helicobacter pylori.
abstract: Helicobacter pylori is associated with various diseases of the upper gastrointestinal tract, such as gastric inflammation and duodenal and gastric ulcers. The aim of the study was to assess anti-H. pylori effects of the sesquiterpene lactone dehydrocostus lactone (DCL) from Magnolia sieboldii leaves, compared to commercial pure DCL, two previously known sesquiterpene lactones (costunolide and parthenolide), (-)-epigallocatechin gallate, and four antibiotics. The antibacterial activity of natural DCL toward antibiotic-susceptible H. pylori ATCC 700392 and H. pylori ATCC 700824 strains (MIC, 4.9 and 4.4 mg/L) was similar to that of commercial DCL and was more effective than costunolide, parthenolide, and EGCG. The activity of DCL was slightly lower than that of metronidazole (MIC, 1.10 and 1.07 mg/L). The antibacterial activity of DCL was virtually identical toward susceptible and resistant strains, even though resistance to amoxicillin (MIC, 11.1 mg/L for PED 503G strain), clarithromycin (49.8 mg/L for PED 3582GA strain), metronidazole (21.6 mg/L for H. pylori ATCC 43504 strain; 71.1 mg/L for 221 strain), or tetracycline (14.2 mg/L for B strain) was observed. This finding indicates that DCL and the antibiotics do not share a common mode of action. The bactericidal activity of DCL toward H. pylori ATCC 43504 was not affected by pH values examined (4.0-7.0). DCL caused considerable conversion to coccoid form (94 versus 49% at 8 and 4 mg/L of DCL for 48 h). The Western blot analysis revealed that urease subunits (UreA and UreB) of H. pylori ATCC 43504 were not affected by 10 mM of DCL, whereas UreA monomer band completely disappeared at 0.1 mM of (-)-epigallocatechin gallate. Global efforts to reduce the level of antibiotics justify further studies on M. sieboldii leaf-derived materials containing DCL as potential antibacterial products or a lead molecule for the prevention or eradication of drug-resistant H. pylori.
authors.affiliation: Interdisciplinary Program in Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul, South Korea.
authors.forename: Hyun-Kyung
authors.initials: HK
authors.lastname: Lee
authors.affiliation: Biomodulation Major, Department of Agricultural Biotechnology, Seoul National University, Seoul, South Korea.
authors.forename: Ha Eun
authors.initials: HE
authors.lastname: Song
authors.affiliation: Interdisciplinary Program in Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul, South Korea.
authors.forename: Haeng-Byung
authors.initials: HB
authors.lastname: Lee
authors.affiliation: Halla Botanical Garden, Jeju City, Jeju, South Korea.
authors.forename: Cheol-Soo
authors.initials: CS
authors.lastname: Kim
authors.affiliation: Department of Chemistry and Biomolecular Science, Gifu University, Gifu, Japan.
authors.forename: Mamoru
authors.initials: M
authors.lastname: Koketsu
authors.affiliation: Department of Plant Biotechnology and Biotransformation, Faculty of Biology, Ho Chi Minh City University of Science, Vietnam National University, Ho Chi Minh City, Vietnam.
authors.forename: Luong Thi My
authors.initials: LT
authors.lastname: Ngan
authors.affiliation: Biomodulation Major, Department of Agricultural Biotechnology, Seoul National University, Seoul, South Korea.
authors.forename: Young-Joon
authors.initials: YJ
authors.lastname: Ahn
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D024881:Drug Resistance, Bacterial; D016480:Helicobacter pylori / Q000187:drug effects* / Q000648:ultrastructure; D006863:Hydrogen-Ion Concentration; D007783:Lactones / Q000737:chemistry / Q000494:pharmacology*; D031566:Magnolia / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability; D015394:Molecular Structure; D018515:Plant Leaves / Q000737:chemistry*; D012717:Sesquiterpenes / Q000737:chemistry / Q000494:pharmacology*; D014510:Urease / Q000037:antagonists & inhibitors
chemical_list: D000900:Anti-Bacterial Agents; D007783:Lactones; D012717:Sesquiterpenes; C083030:dehydrocostus lactone; D014510:Urease
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