title: Flow cytometry in mastocytosis: utility as a diagnostic and prognostic tool.
abstract: This article presents information for the identification and characterization of mast cells from bone marrow and other tissues using multiparametric flow cytometry. In addition, it provides guidelines for the application of this technique in the subclassification of systemic mastocytosis and assessment of the long-term prognosis of patients individually.
authors.affiliation: Mastocytosis Unit of Castilla La-Mancha, Hospital Virgen del Valle, Toledo, Spain; Spanish Network on Mastocytosis (REMA), Toledo, Spain. Electronic address: lsmunoz@sescam.jccm.es.
authors.forename: Laura
authors.initials: L
authors.lastname: Sánchez-Muñoz
authors.affiliation: Spanish Network on Mastocytosis (REMA), Toledo, Spain; Departments of Cytometry and Medicine, Centro de Investigación del Cáncer, (IBMCC-CSIC/USAL and IBSAL) University of Salamanca, Salamanca, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Teodosio
authors.affiliation: Mastocytosis Unit of Castilla La-Mancha, Hospital Virgen del Valle, Toledo, Spain; Spanish Network on Mastocytosis (REMA), Toledo, Spain.
authors.forename: Jose Mario T
authors.initials: JM
authors.lastname: Morgado
authors.affiliation: Section of Hematology, Department of Medicine, University of Verona, Verona, Italy.
authors.forename: Omar
authors.initials: O
authors.lastname: Perbellini
authors.affiliation: Spanish Network on Mastocytosis (REMA), Toledo, Spain; Departments of Cytometry and Medicine, Centro de Investigación del Cáncer, (IBMCC-CSIC/USAL and IBSAL) University of Salamanca, Salamanca, Spain.
authors.forename: Andrea
authors.initials: A
authors.lastname: Mayado
authors.affiliation: Mastocytosis Unit of Castilla La-Mancha, Hospital Virgen del Valle, Toledo, Spain; Spanish Network on Mastocytosis (REMA), Toledo, Spain.
authors.forename: Ivan
authors.initials: I
authors.lastname: Alvarez-Twose
authors.affiliation: Mastocytosis Unit of Castilla La-Mancha, Hospital Virgen del Valle, Toledo, Spain; Spanish Network on Mastocytosis (REMA), Toledo, Spain.
authors.forename: Almudena
authors.initials: A
authors.lastname: Matito
authors.affiliation: Spanish Network on Mastocytosis (REMA), Toledo, Spain; Departments of Cytometry and Medicine, Centro de Investigación del Cáncer, (IBMCC-CSIC/USAL and IBSAL) University of Salamanca, Salamanca, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Jara-Acevedo
authors.affiliation: Spanish Network on Mastocytosis (REMA), Toledo, Spain; Departments of Cytometry and Medicine, Centro de Investigación del Cáncer, (IBMCC-CSIC/USAL and IBSAL) University of Salamanca, Salamanca, Spain.
authors.forename: Andrés C
authors.initials: AC
authors.lastname: García-Montero
authors.affiliation: Spanish Network on Mastocytosis (REMA), Toledo, Spain; Departments of Cytometry and Medicine, Centro de Investigación del Cáncer, (IBMCC-CSIC/USAL and IBSAL) University of Salamanca, Salamanca, Spain.
authors.forename: Alberto
authors.initials: A
authors.lastname: Orfao
authors.affiliation: Spanish Network on Mastocytosis (REMA), Toledo, Spain; Departments of Cytometry and Medicine, Centro de Investigación del Cáncer, (IBMCC-CSIC/USAL and IBSAL) University of Salamanca, Salamanca, Spain.
authors.forename: Luis
authors.initials: L
authors.lastname: Escribano
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000235:genetics / Q000276:immunology; D001853:Bone Marrow / Q000276:immunology / Q000473:pathology*; D005434:Flow Cytometry; D015870:Gene Expression; D006801:Humans; D016130:Immunophenotyping; D008407:Mast Cells / Q000276:immunology / Q000473:pathology*; D008415:Mastocytosis / Q000175:diagnosis* / Q000235:genetics / Q000276:immunology / Q000473:pathology; D009154:Mutation; D011379:Prognosis; D019009:Proto-Oncogene Proteins c-kit / Q000235:genetics / Q000276:immunology
chemical_list: D015703:Antigens, CD; D019009:Proto-Oncogene Proteins c-kit
keywords: Bone marrow; Cell purification; Classification; Diagnosis; Flow cytometry; Mast cells; Mastocytosis; Prognosis