title: Cell-type-specific Jumonji histone demethylase gene expression in the healthy rat CNS: detection by a novel flow cytometry method.
abstract: Our understanding of how histone demethylation contributes to the regulation of basal gene expression in the brain is largely unknown in any injury model, and especially in the healthy adult brain. Although Jumonji genes are often regulated transcriptionally, cell-specific gene expression of Jumonji histone demethylases in the brain remains poorly understood. Thus, in the present study we profiled the mRNA levels of 26 Jumonji genes in microglia (CD11b+), neurons (NeuN+) and astrocytes (GFAP+) from the healthy adult rat brain. We optimized a method combining a mZBF (modified zinc-based fixative) and FCM (flow cytometry) to simultaneously sort cells from non-transgenic animals. We evaluated cell-surface, intracellular and nuclear proteins, including histones, as well as messenger- and micro-RNAs in different cell types simultaneously from a single-sorted sample. We found that 12 Jumonji genes were differentially expressed between adult microglia, neurons and astrocytes. While JMJD2D was neuron-restricted, PHF8 and JMJD1C were expressed in all three cell types although the expression was highest in neurons. JMJD3 and JMJD5 were expressed in all cell types, but were highly enriched in microglia; astrocytes had the lowest expression of UTX and JHDM1D. Levels of global H3K27 (H3 lysine 27) methylation varied among cell types and appeared to be lowest in microglia, indicating that differences in basal gene expression of specific Jumonji histone demethylases may contribute to cell-specific gene expression in the CNS (central nervous system). This multiparametric technique will be valuable for simultaneously assaying chromatin modifications and gene regulation in the adult CNS.
authors.affiliation: *Department of Comparative Biosciences, University of Wisconsin, 2015 Linden Dr., Madison, WI 53706, U.S.A.
authors.forename: Stephanie M C
authors.initials: SM
authors.lastname: Smith
authors.affiliation: *Department of Comparative Biosciences, University of Wisconsin, 2015 Linden Dr., Madison, WI 53706, U.S.A.
authors.forename: Rebecca S
authors.initials: RS
authors.lastname: Kimyon
authors.affiliation: *Department of Comparative Biosciences, University of Wisconsin, 2015 Linden Dr., Madison, WI 53706, U.S.A.
authors.forename: Jyoti J
authors.initials: JJ
authors.lastname: Watters
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D039481:CD11b Antigen / Q000378:metabolism; D002490:Central Nervous System / Q000166:cytology*; D027342:Excitatory Amino Acid Transporter 2 / Q000378:metabolism; D005434:Flow Cytometry; D056484:Jumonji Domain-Containing Histone Demethylases / Q000378:metabolism*; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009457:Neuroglia / Q000378:metabolism; D009474:Neurons / Q000201:enzymology*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D039481:CD11b Antigen; D027342:Excitatory Amino Acid Transporter 2; D035683:MicroRNAs; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; C493395:Slc1a2 protein, rat; D056484:Jumonji Domain-Containing Histone Demethylases
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