title: Development of an automated multi-injection shotgun lipidomics approach using a triple quadrupole mass spectrometer.
abstract: Shotgun lipidomics is a well-suited approach to monitor lipid alterations due to its ability to scan for varying lipid types on a global, class and individual species level. However, the ability to perform high-throughput shotgun lipidomics has remained challenging due to time-consuming data processing and hardware limitations. To increase the throughput nature of shotgun lipidomics, an automated shotgun lipidomics approach is described utilizing conventional low flow gradient liquid chromatography (LC) analysis (post-injection) coupled with multiple sample injections per sample (on a lipid scan per injection basis). The proposed automated multi-injection approach resulted in a reproducible lipid scanning period of 2.5 min (in a 4.5 min total data acquisition period), thereby providing a sufficient scanning period for performing either mass spectrometric or tandem mass spectrometric analyses. In addition to being simple, robust and reproducible, this approach was also constructed to be cost-effective by using common LC instrumentation and customizable as the data acquisition period can be tailored to perform different scan types, period lengths and scan numbers. Combined with a strategy to create multiple lipid-specific aliquots per sample, the overall approach provides a simple and efficient platform to perform high-throughput lipid profiling.
authors.affiliation: Hollings Marine Laboratory, National Institute of Standards and Technology, 331 Fort Johnson Road, Charleston, SC, 29412, USA, john.bowden@nist.gov.
authors.forename: John A
authors.initials: JA
authors.lastname: Bowden
authors.forename: Jackie T
authors.initials: JT
authors.lastname: Bangma
authors.forename: John R
authors.initials: JR
authors.lastname: Kucklick
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000592:standards; D006801:Humans; D008055:Lipids / Q000097:blood* / Q000302:isolation & purification; D012015:Reference Standards; D021241:Spectrometry, Mass, Electrospray Ionization / Q000592:standards; D053719:Tandem Mass Spectrometry / Q000592:standards
chemical_list: D008055:Lipids
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