title: Vibrio cholerae O1 Ogawa El Tor strains with the ctxB7 allele driving cholera outbreaks in south-western India in 2012.
abstract: Cholera has been a recurrent epidemic disease in human populations for the past 200years. We present herein a comparative characterization of clinical Vibrio cholerae strains isolated from two consecutive cholera outbreaks in 2012 and associated environmental strains from western India. The clinical and toxigenic environmental isolates were identified as hybrid V. cholerae O1, serotype Ogawa, biotype El Tor carrying the variant ctxB7 allele. Partial sequences of SXT integrase from the isolates revealed 100% identity to ICEVchInd5 (Sevagram, India, 1994) and VC1786ICE (Haiti, 2013). The full clonal relationship of the strains established by RAPD, Box PCR, ERIC PCR and MLST (pyrH, recA and rpoA) analyses, and the short time between the two outbreaks, strongly supported that both outbreaks were due to a single strain. The study corroborated that faecal contamination of the potable water supply was the main reason for the first outbreak, which further spread to other areas and resulted in the second outbreak. The study concluded that the circulating El Tor variant strains of epidemic potential in the region can be a serious concern in the future.
authors.affiliation: Jawaharlal Nehru University, New Delhi 110067, India.
authors.forename: P
authors.initials: P
authors.lastname: Kumar
authors.affiliation: Jawaharlal Nehru University, New Delhi 110067, India.
authors.forename: D K
authors.initials: DK
authors.lastname: Mishra
authors.affiliation: SVN Govt. Medical College, Yavatmal, India.
authors.forename: D G
authors.initials: DG
authors.lastname: Deshmukh
authors.affiliation: Defence Research and Development Establishment, Gwalior 474002, India.
authors.forename: M
authors.initials: M
authors.lastname: Jain
authors.affiliation: SVN Govt. Medical College, Yavatmal, India.
authors.forename: A M
authors.initials: AM
authors.lastname: Zade
authors.affiliation: SVN Govt. Medical College, Yavatmal, India.
authors.forename: K V
authors.initials: KV
authors.lastname: Ingole
authors.affiliation: Defence Research and Development Establishment, Gwalior 474002, India.
authors.forename: A K
authors.initials: AK
authors.lastname: Goel
authors.affiliation: Jawaharlal Nehru University, New Delhi 110067, India. Electronic address: pkyadava1953@gmail.com.
authors.forename: P K
authors.initials: PK
authors.lastname: Yadava
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D002771:Cholera / Q000453:epidemiology* / Q000382:microbiology; D004196:Disease Outbreaks; D060766:Drinking Water / Q000382:microbiology*; D006801:Humans; D007194:India / Q000453:epidemiology; D019426:Integrases / Q000235:genetics*; D058885:Multilocus Sequence Typing; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D041241:Vibrio cholerae O1 / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D001426:Bacterial Proteins; D060766:Drinking Water; D019426:Integrases
keywords: Cholera; El Tor variant biotype; MLST; Outbreak; Vibrio cholerae; ctxB7