title: Repeated dose and reproductive/developmental toxicity of perfluorododecanoic acid in rats.
abstract: Perfluoroalkyl carboxylic acids (PFCAs) are a series of environmental contaminants that have received attention because of their possible adverse effects on wildlife and human health. Although many toxicological studies have been performed on perfluorooctanoic acid with carbon chain length C8, available toxicity data on PFCAs with longer chains are still insufficient to evaluate their hazard. A combined repeated dose and reproductive/developmental toxicity screening study for perfluorododecanoic acid (PFDoA; C12) was conducted in accordance with OECD guideline 422 to fill these toxicity data gaps. PFDoA was administered by gavage to male and female rats at 0.1, 0.5, or 2.5 mg/kg/day. The administration of PFDoA at 0.5 and 2.5 mg/kg/day for 42-47 days mainly affected the liver, in which hypertrophy, necrosis, and inflammatory cholestasis were noted. Body weight gain was markedly inhibited in the 2.5 mg/kg/day group, and a decrease in hematopoiesis in the bone marrow and atrophic changes in the spleen, thymus, and adrenal gland were also observed. Regarding reproductive/developmental toxicity, various histopathological changes, including decreased spermatid and spermatozoa counts, were observed in the male reproductive organs, while continuous diestrous was observed in the females of the 2.5 mg/kg/day group. Seven of twelve females receiving 2.5 mg/kg/day died during late pregnancy while four other females in this group did not deliver live pups. No reproductive or developmental parameters changed at 0.1 or 0.5 mg/kg/day. Based on these results, the NOAELs of PFDoA were concluded to be 0.1 mg/kg/day for repeated dose toxicity and 0.5 mg/kg/day for reproductive/developmental toxicity.
authors.affiliation: Division of Risk Assessment, Biological Safety Research Center, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-Ku, Tokyo, 158-8501, Japan.
authors.forename: Hina
authors.initials: H
authors.lastname: Kato
authors.affiliation: Safety Research Institute for Chemical Compounds Co., Ltd., 363-24 Sin-ei, Kiyota-ku, Sapporo, Hokkaido, 004-0839, Japan.
authors.forename: Sakiko
authors.initials: S
authors.lastname: Fujii
authors.affiliation: Division of Risk Assessment, Biological Safety Research Center, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-Ku, Tokyo, 158-8501, Japan.
authors.forename: Mika
authors.initials: M
authors.lastname: Takahashi
authors.affiliation: Division of Risk Assessment, Biological Safety Research Center, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-Ku, Tokyo, 158-8501, Japan.
authors.forename: Mariko
authors.initials: M
authors.lastname: Matsumoto
authors.affiliation: Division of Risk Assessment, Biological Safety Research Center, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-Ku, Tokyo, 158-8501, Japan.
authors.forename: Mutsuko
authors.initials: M
authors.lastname: Hirata-Koizumi
authors.affiliation: Division of Risk Assessment, Biological Safety Research Center, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-Ku, Tokyo, 158-8501, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Ono
authors.affiliation: Division of Risk Assessment, Biological Safety Research Center, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-Ku, Tokyo, 158-8501, Japan.
authors.forename: Akihiko
authors.initials: A
authors.lastname: Hirose
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D004785:Environmental Pollutants / Q000633:toxicity*; D005260:Female; D005466:Fluorocarbons; D006984:Hypertrophy; D007850:Lauric Acids / Q000633:toxicity*; D008099:Liver / Q000187:drug effects* / Q000473:pathology; D008297:Male; D018811:Maternal Exposure / Q000009:adverse effects*; D009336:Necrosis; D019055:No-Observed-Adverse-Effect Level; D018812:Paternal Exposure / Q000009:adverse effects*; D011247:Pregnancy; D051381:Rats; D012098:Reproduction / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects
chemical_list: D004785:Environmental Pollutants; D005466:Fluorocarbons; D007850:Lauric Acids; C522391:perfluorododecanoic acid
keywords: developmental toxicity; perfluoroalkyl carboxylic acid; perfluorododecanoic acid; rat; repeated dose toxicity; reproductive toxicity; screening test