title: Over-expression of PsGPD, a mushroom glyceraldehyde-3-phosphate dehydrogenase gene, enhances salt tolerance in rice plants.
abstract: Transgenic potatoes expressing glyceraldehyde-3-phosphate dehydrogenase (GPD), isolated from the oyster mushroom, Pleurotus sajor-caju, had increased tolerance to salt stress (Jeong et al. Biochem Biophys Res Commun 278:192-196, 2000). To examine the physiological mechanisms enhancing salt tolerance in GPD-transgenic rice plants, the salt tolerance of five GPD transgenic rice lines (T1-T5) derived from Dongjin rice cultivar were evaluated in a fixed 150 mM saline environment in comparison to two known wild-type rice cultivars, Dongjin (salt sensitive) and Pokali (salt tolerant). Transgenic lines, T2, T3, and T5, had a substantial increase in biomass and relative water content compared to Dongjin. Stomatal conductance and osmotic potential were higher in the GPD transgenic lines and were similar to those in Pokali. The results are discussed based on the comparative physiological response of GPD transgenic lines with those of the salt-sensitive and salt-tolerant rice cultivars.
authors.affiliation: National Academy of Agricultural Sciences, Rural Development Administration, Suwon, 441-707, Republic of Korea.
authors.forename: Jung-Il
authors.initials: JI
authors.lastname: Cho
authors.forename: Hye-Min
authors.initials: HM
authors.lastname: Lim
authors.forename: Zamin Shaheed
authors.initials: ZS
authors.lastname: Siddiqui
authors.forename: Sung-Han
authors.initials: SH
authors.lastname: Park
authors.forename: A-Ram
authors.initials: AR
authors.lastname: Kim
authors.forename: Taek-Ryoun
authors.initials: TR
authors.lastname: Kwon
authors.forename: Seong-Kon
authors.initials: SK
authors.lastname: Lee
authors.forename: Soo-Chul
authors.initials: SC
authors.lastname: Park
authors.forename: Mi-Jeong
authors.initials: MJ
authors.lastname: Jeong
authors.forename: Gang-Seob
authors.initials: GS
authors.lastname: Lee
mesh_terms: D018533:Biomass; D004269:DNA, Bacterial / Q000235:genetics; D003624:Darkness; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics* / Q000378:metabolism; D012275:Oryza / Q000235:genetics / Q000502:physiology*; D009995:Osmosis; D054046:Plant Stomata / Q000502:physiology; D030821:Plants, Genetically Modified; D020076:Pleurotus / Q000201:enzymology* / Q000235:genetics*; D055049:Salt Tolerance; D013312:Stress, Physiological; D014867:Water
chemical_list: D004269:DNA, Bacterial; C036483:T-DNA; D014867:Water; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases