title: FISH analysis of translocations induced by chronic exposure to Sr radioisotopes: second set of analysis of the Techa River Cohort.
abstract: Fluorescent in situ hybridisation analysis of stable translocations was performed for 26 residents living along the Techa River (Russia), who were predominantly (95%) exposed to ingested strontium radioisotopes ((89)Sr and (90)Sr) resulting in exposure of their red bone marrow (RBM). Analysis was conducted at the Urals Research Center for Radiation Medicine, Public Health England and Leiden University Medical Center. Each laboratory scored 1000 cells per donor, which resulted in ∼1000 genome equivalents (GE) per donor. The age-dependent spontaneous level of translocations for each donor was evaluated on the basis of data published by Sigurdson et al. (International study of factors affecting human chromosome. Mutat. Res. 2008;652: :112-121). Reconstruction of doses was performed with the 'Techa River Dosimetry System' developed in 2009. In the studied donors, the range of individual cumulated RBM dose was from 0.3 to 3.7 Gy. Analysis of the yield of stable translocations dependent on the individual RBM dose from (89,90)Sr showed a linear dose-response relationship of 0.007 ± 0.002 translocation/GE cell/Gy (R = 0.61, p = 0.001). This set of results was in a good agreement with the previous data reported for 18 donors by Vozilova et al. (Preliminary FISH-based assessment of external dose for residents exposed on the Techa River.
authors.affiliation: Urals Research Center for Radiation Medicine (URCRM), Vorovsky str. 68A, Chelyabinsk 454076, Russia vozilova@urcrm.ru.
authors.forename: Alexandra V
authors.initials: AV
authors.lastname: Vozilova
authors.affiliation: Urals Research Center for Radiation Medicine (URCRM), Vorovsky str. 68A, Chelyabinsk 454076, Russia.
authors.forename: Natalia B
authors.initials: NB
authors.lastname: Shagina
authors.affiliation: Urals Research Center for Radiation Medicine (URCRM), Vorovsky str. 68A, Chelyabinsk 454076, Russia.
authors.forename: Marina O
authors.initials: MO
authors.lastname: Degteva
authors.affiliation: Public Health England (PHE), Chilton, Didcot, Oxon OX11 ORQ, UK.
authors.forename: Jayne
authors.initials: J
authors.lastname: Moquet
authors.affiliation: Public Health England (PHE), Chilton, Didcot, Oxon OX11 ORQ, UK.
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Ainsbury
authors.affiliation: Department of Toxicogenetics, Leiden University Medical Center (LUMC), Einthovenweg 20, Leiden 2300RC, The Netherlands.
authors.forename: Firouz
authors.initials: F
authors.lastname: Darroudi
mesh_terms: D000368:Aged; D001853:Bone Marrow / Q000528:radiation effects*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008875:Middle Aged; D011829:Radiation Dosage; D011834:Radiation Monitoring / Q000379:methods*; D012062:Relative Biological Effectiveness; D012424:Rural Population; D012426:Russia; D013326:Strontium Radioisotopes / Q000009:adverse effects* / Q000032:analysis; D014178:Translocation, Genetic / Q000235:genetics* / Q000528:radiation effects*
chemical_list: D013326:Strontium Radioisotopes