title: Microbacterium kyungheense sp. nov. and Microbacterium jejuense sp. nov., isolated from salty soil.
abstract: Two novel strains, THG-C26(T) and THG-C31(T), were characterized using a polyphasic approach to determine their taxonomic positions. These two isolates were aerobic, Gram-stain-positive, non-motile, non-spore-forming and rod-shaped. 16S rRNA gene sequences and phenotypic features including chemotaxonomic characteristics indicated that the two isolates clearly represented members of the genus Microbacterium. The quinone systems of strains THG-C26(T) and THG-C31(T) contained MK-12/MK-13 as major menaquinones. The diamino acid in cell-wall hydrolysates of the two strains was ornithine. The major fatty acids were iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0. The polyamine pattern had spermidine as the predominant component. The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol and unidentified glycolipids. Phenotypic characteristics supported the affiliation of strains THG-C26(T) and THG-C31(T) to the genus Microbacterium. Chemotaxonomic data and DNA-DNA relatedness values allowed differentiation of these strains from other species of the genus Microbacterium with validly published names. Strains THG-C26(T) and THG-C31(T) showed highest 16S rRNA gene sequence similarities with Microbacterium resistens DMMZ 1710(T) (98.5%) and Microbacterium trichothecenolyticum IFO 15077(T) (98.8%), respectively, and the 16S rRNA gene sequence similarity between them was 99.0%. DNA-DNA hybridization values between the novel isolates and strains of other species of the genus Microbacterium with validly published names were 4-25%. Therefore, strains THG-C26(T) and THG-C31(T) are considered to represent two novel species of the genus Microbacterium, for which the names Microbacterium kyungheense sp. nov. [type strain THG-C26(T) ( = KACC 17124(T) = JCM 18735(T))] and Microbacterium jejuense sp. nov. [type strain THG-C31(T) ( = KACC 17123(T) = JCM 18734(T))] are proposed.
authors.affiliation: Department of Marine Biotechnology, Anyang University, Incheon 417-833, Republic of Korea.
authors.forename: MooChang
authors.initials: M
authors.lastname: Kook
authors.affiliation: Department of Oriental Medicinal Material & Processing College of Life science, Kyung Hee University, Yongin, Kyunggi 446-701, Republic of Korea.
authors.forename: Heung-Min
authors.initials: HM
authors.lastname: Son
authors.affiliation: Department of Oriental Medicinal Material & Processing College of Life science, Kyung Hee University, Yongin, Kyunggi 446-701, Republic of Korea.
authors.forename: Tae-Hoo
authors.initials: TH
authors.lastname: Yi
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D006017:Glycolipids / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D009952:Ornithine / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013095:Spermidine / Q000737:chemistry; D024482:Vitamin K 2 / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D006017:Glycolipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D009952:Ornithine; D013095:Spermidine