title: Immunologic and mass-spectrometric estimates of SHBG concentrations in healthy women.
abstract: OBJECTIVE
  Sex-hormone binding globulin (SHBG) concentrations across the adult female lifespan are not well defined. To address this knowledge gap, SHBG was quantified by both immunological and criterion methods, viz, mass spectrometry (MS).
  
  
  METHODS
  
  
  
  METHODS
  Center for Translational Science Activities (CTSA).
  
  
  METHODS
  Healthy nonpregnant women (N=120) ages 21 to 79 years.
  
  
  RESULTS
  SHBG, testosterone (T), estradiol (E2) and estrone (E1) each determined by MS. Uni- and multivariate regression of SHBG concentrations on age, body mass index (BMI), total and visceral abdominal fat (TAF, AVF), albumin, glucose, insulin, sex steroids, selected cytokines, blood pressure, and lipids.
  
  
  RESULTS
  By univariate regression, MS-estimated SHBG correlated negatively with BMI, TAF, AVF, insulin, free T and bioavailable T (bio T) (each P≤10(-4)), but not with blood pressure or lipids. By stepwise multivariate regression analysis, free and total T (both positive) and bio T (negative) were correlated with SHBG in all 4 assays (each P<10(-15), R(2)≥0.481). In addition, TAF and BMI were negatively associated with SHBG (P≤0.0066) in 2 SHBG assays, and estrone and IL-8 with SHBG weakly (P≤0.035) in one SHBG assay each. When nonsignificant cytokines were excluded, SHBG was jointly associated with AVF, total T and HDL (P<10(-9), R(2)=0.358).
  
  
  CONCLUSIONS
  According to MS, three metabolic factors, T, AVF and HDL, together explain more than one-third of the interindividual variation in SHBG levels. We speculate that these measures reflect insulin action.
authors.affiliation: Endocrine Research Unit, Mayo School of Graduate Medical Education, Mayo Clinic, Rochester, MN 55905. Electronic address: veldhuis.johannes@mayo.edu.
authors.forename: Johannes D
authors.initials: JD
authors.lastname: Veldhuis
authors.affiliation: Immunochemical Laboratory, Center for Translational Science Activities, Mayo Clinic, Rochester, MN 55905.
authors.forename: Roy B
authors.initials: RB
authors.lastname: Dyer
authors.affiliation: Immunochemical Laboratory, Center for Translational Science Activities, Mayo Clinic, Rochester, MN 55905; Department of Neurology, Center for Translational Science Activities, Mayo Clinic, Rochester, MN 55905.
authors.forename: Sergey A
authors.initials: SA
authors.lastname: Trushin
authors.affiliation: Laboratory Medicine and Pathology, Center for Translational Science Activities, Mayo Clinic, Rochester, MN 55905.
authors.forename: Olga P
authors.initials: OP
authors.lastname: Bondar
authors.affiliation: Laboratory Medicine and Pathology, Center for Translational Science Activities, Mayo Clinic, Rochester, MN 55905.
authors.forename: Ravinder J
authors.initials: RJ
authors.lastname: Singh
authors.affiliation: Laboratory Medicine and Pathology, Center for Translational Science Activities, Mayo Clinic, Rochester, MN 55905.
authors.forename: George G
authors.initials: GG
authors.lastname: Klee
mesh_terms: D000328:Adult; D000368:Aged; D001786:Blood Glucose / Q000378:metabolism; D015992:Body Mass Index; D008076:Cholesterol, HDL / Q000097:blood; D004958:Estradiol / Q000097:blood; D004970:Estrone / Q000097:blood; D005260:Female; D006801:Humans; D007328:Insulin / Q000097:blood; D007378:Interleukins / Q000097:blood; D050152:Intra-Abdominal Fat / Q000378:metabolism*; D013058:Mass Spectrometry; D008593:Menopause; D008875:Middle Aged; D012709:Serum Albumin / Q000378:metabolism; D012738:Sex Hormone-Binding Globulin / Q000378:metabolism*; D013739:Testosterone / Q000097:blood*; D014280:Triglycerides / Q000097:blood; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D001786:Blood Glucose; D008076:Cholesterol, HDL; D007328:Insulin; D007378:Interleukins; D012709:Serum Albumin; D012738:Sex Hormone-Binding Globulin; D014280:Triglycerides; D014409:Tumor Necrosis Factor-alpha; D004970:Estrone; D013739:Testosterone; D004958:Estradiol
keywords: Body composition; Female; Insulin; Obesity; Sex steroid
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