title: Stretching desmin filaments with receding meniscus reveals large axial tensile strength.
abstract: Desmin forms the intermediate filament system of muscle cells where it plays important role in maintaining mechanical integrity and elasticity. Although the importance of intermediate-filament elasticity in cellular mechanics is being increasingly recognized, the molecular basis of desmin's elasticity is not fully understood. We explored desmin elasticity by molecular combing with forces calculated to be as large as 4nN. Average filament contour length increased 1.55-fold axial on average. Molecular combing together with EGTA-treatment caused the fragmentation of the filament into short, 60 to 120-nm-long and 4-nm-wide structures. The fragments display a surface periodicity of 38nm, suggesting that they are composed of laterally attached desmin dimers. The axis of the fragments may deviate significantly from that of the overstretched filament, indicating that they have a large orientational freedom in spite of being axially interconnected. The emergence of protofibril fragments thus suggests that the interconnecting head or tail domains of coiled-coil desmin dimers are load-bearing elements during axial stretch.
authors.affiliation: Department of Biophysics and Radiation Biology, MTA-SE Molecular Biophysics Research Group, Semmelweis University, 1094 Budapest, Tűzoltó u. 37-47, Hungary. Electronic address: balazs.kiss@eok.sote.hu.
authors.forename: Balázs
authors.initials: B
authors.lastname: Kiss
authors.affiliation: Department of Biophysics and Radiation Biology, MTA-SE Molecular Biophysics Research Group, Semmelweis University, 1094 Budapest, Tűzoltó u. 37-47, Hungary.
authors.forename: Miklós S Z
authors.initials: MS
authors.lastname: Kellermayer
mesh_terms: D000818:Animals; D003893:Desmin / Q000737:chemistry*; D004533:Egtazic Acid / Q000737:chemistry; D004548:Elasticity; D007382:Intermediate Filaments / Q000737:chemistry; D018625:Microscopy, Atomic Force; D013718:Tensile Strength
chemical_list: D003893:Desmin; D004533:Egtazic Acid
keywords: Atomic force microscopy; Desmin; Elasticity; Receding meniscus; Unit length filament