title: pH sensitive thiolated cationic hydrogel for oral insulin delivery.
abstract: The objective of this work is to study the efficacy of pH sensitive thiolated Polydimethylaminoethylmethacrylate for oral delivery of insulin. Synthesis of pH sensitive thiolated Polydimethylaminoethylmethacrylate (PDCPA) was carried out by crosslinking Polymethacrylic acid with thiolated Polydimethylaminoethylmethacrylate (PDCys) via carbodiimide chemistry. Prior to in vivo experiment, various physicochemical and biological characterisation were carried out to evaluate the efficacy of PDCPA. Modification was confirmed by IR and NMR spectroscopy. The particle size was found to be 284 nm with a zeta potential of 37.3+/-1.58 mV. Texture analyser measurements showed that PDCPA is more mucoadhesive than the parent polymer. Transepithelial electrical measurements showed a reduction of greater than 50% on incubation with PDCPA particles. Permeation studies showed that PDCPA is more permeable than the parent polymer. On in vivo evaluation on male diabetic rats, insulin loaded PDCPA exhibited a blood glucose reduction of 19%.
authors.forename: T A
authors.initials: TA
authors.lastname: Sonia
authors.forename: Chandra P
authors.initials: CP
authors.lastname: Sharma
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D018938:Caco-2 Cells; D002412:Cations; D003692:Delayed-Action Preparations / Q000008:administration & dosage / Q000737:chemistry; D003921:Diabetes Mellitus, Experimental / Q000097:blood* / Q000175:diagnosis / Q000188:drug therapy*; D006801:Humans; D020100:Hydrogels / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007004:Hypoglycemic Agents / Q000008:administration & dosage / Q000737:chemistry; D007328:Insulin / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics*; D008297:Male; D008657:Metabolic Clearance Rate; D053769:Nanocapsules / Q000008:administration & dosage / Q000737:chemistry* / Q000648:ultrastructure; D017208:Rats, Wistar; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D016896:Treatment Outcome
chemical_list: D001786:Blood Glucose; D002412:Cations; D003692:Delayed-Action Preparations; D020100:Hydrogels; D007004:Hypoglycemic Agents; D007328:Insulin; D053769:Nanocapsules; D013438:Sulfhydryl Compounds