title: The combination of strong expression of ZNF143 and high MIB-1 labelling index independently predicts shorter disease-specific survival in lung adenocarcinoma.
abstract: BACKGROUND
  The transcription factor, zinc finger protein 143 (ZNF143), positively regulates many cell-cycle-related genes. The ZNF143 would show high expression of multiple solid tumours related closely to cancer cell growth, similar to the widely accepted Ki67 (MIB-1) protein, but the underlying mechanisms for ZNF143 remain unclear. We investigated the association of ZNF143 expression with clinicopathological features and prognoses of patients with lung adenocarcinoma.
  
  
  METHODS
  Expressions of ZNF143 and MIB-1 were immunohistochemically analysed in 183 paraffin-embedded tumour samples of patients with lung adenocarcinoma. The ZNF143 expression was considered to be strong when >30% of the cancer cells demonstrated positive staining.
  
  
  RESULTS
  Strong ZNF143+ expression showed a significantly close relationship to pathologically moderate to poor differentiation and highly invasive characteristics. The ZNF143 positivity potentially induced cell growth of lung adenocarcinoma, correlated significantly with high MIB-1 labelling index (⩾10%). Univariate and multivariate analyses demonstrated that both strong ZNF143+ and the high MIB-1 index group have only and significantly worse survival rates.
  
  
  CONCLUSIONS
  The combination of strong ZNF143 expression and high MIB-1 index potentially predicts high proliferating activity and poor prognosis in patients with lung adenocarcinoma, and may offer a therapeutic target against ZNF143.
authors.affiliation: Department of Health Policy and Management, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Kawatsu
authors.affiliation: 1] Department of Pathology and Cell Biology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan [2] Department of Urology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Kitada
authors.affiliation: Department of Second Department of Surgery, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Uramoto
authors.affiliation: 1] Department of Pathology and Cell Biology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan [2] Department of Medical Oncology, The First Hospital of China Medical University, Shenyang 110001, People's Republic of China.
authors.forename: L
authors.initials: L
authors.lastname: Zhi
authors.affiliation: Department of Health Policy and Management, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Takeda
authors.affiliation: Department of Health Policy and Management, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kimura
authors.affiliation: Department of Health Policy and Management, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Horie
authors.affiliation: Department of Second Department of Surgery, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan.
authors.forename: F
authors.initials: F
authors.lastname: Tanaka
authors.affiliation: 1] Department of Pathology and Cell Biology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan [2] Laboratory of Pathology, Fukuoka Wajiro Hospital, Fukuoka 811-0213, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Sasaguri
authors.affiliation: Department of Occupational Pneumology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Izumi
authors.affiliation: The President Laboratory, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Kohno
authors.affiliation: Department of Pathology and Cell Biology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Yamada
mesh_terms: D000230:Adenocarcinoma / Q000737:chemistry* / Q000401:mortality / Q000601:surgery; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000595:Amino Acid Sequence; D002454:Cell Differentiation; D002455:Cell Division; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D019394:Ki-67 Antigen / Q000032:analysis*; D008175:Lung Neoplasms / Q000737:chemistry* / Q000401:mortality / Q000601:surgery; D008297:Male; D008875:Middle Aged; D008940:Mitotic Index; D008969:Molecular Sequence Data; D060787:Neoplasm Grading; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000032:analysis* / Q000276:immunology; D010446:Peptide Fragments / Q000276:immunology; D011379:Prognosis; D012189:Retrospective Studies; D016019:Survival Analysis; D015534:Trans-Activators / Q000032:analysis* / Q000276:immunology; D016896:Treatment Outcome
chemical_list: D019394:Ki-67 Antigen; D009363:Neoplasm Proteins; D010446:Peptide Fragments; D015534:Trans-Activators; C114253:ZNF143 protein, human
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