title: miR-22 inhibits osteosarcoma cell proliferation and migration by targeting HMGB1 and inhibiting HMGB1-mediated autophagy.
abstract: Acquisition of drug-resistant phenotypes is often associated with chemotherapy in osteosarcoma. Studies show that high-mobility group box 1 (HMGB1) plays an important role in facilitating autophagy and promotes drug resistance in osteosarcoma cells. In this study, we determined the targeting role of miR-22 to HMGB1 and the regulation of miR-22 on the HMGB1-mediated cell autophagy and on the cell proliferation, migration, and invasion of osteosarcoma cells. Results demonstrated that miR-22 well paired with the 3'-UTR of HMGB1 downregulated the HMGB1 expression and blocked the HMGB1-mediated autophagy during chemotherapy in osteosarcoma cells in vitro. Further study showed that the blockage of autophagy by miR-22 inhibited the osteosarcoma cell proliferation, migration, and invasion. In summary, this study implied the negative regulation of miR-22 on the HMGB1-mediated autophagy in osteosarcoma cells.
authors.affiliation: Orthopedics Department, Second Affiliated Hospital of Inner Mongolia Medical University, No.1, Yingfang Road, Huimin District, Hohhot, 010050, China.
authors.forename: Shibing
authors.initials: S
authors.lastname: Guo
authors.forename: Rui
authors.initials: R
authors.lastname: Bai
authors.forename: Wanlin
authors.initials: W
authors.lastname: Liu
authors.forename: Aiqing
authors.initials: A
authors.lastname: Zhao
authors.forename: Zhenqun
authors.initials: Z
authors.lastname: Zhao
authors.forename: Yuxin
authors.initials: Y
authors.lastname: Wang
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
authors.forename: Wenxuan
authors.initials: W
authors.lastname: Wang
mesh_terms: D020413:3' Untranslated Regions; D001343:Autophagy / Q000235:genetics; D001859:Bone Neoplasms / Q000235:genetics* / Q000473:pathology; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D019008:Drug Resistance, Neoplasm / Q000235:genetics; D024243:HMGB1 Protein / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D012516:Osteosarcoma / Q000235:genetics* / Q000473:pathology
chemical_list: D020413:3' Untranslated Regions; D024243:HMGB1 Protein; C548375:MIRN22 microRNA, human; D035683:MicroRNAs
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