title: Absence of association of interleukin-18 gene polymorphisms with primary immune thrombocytopenia in a Chinese Han population.
abstract: Interleukin-18 (IL-18) is an inflammatory cytokine that plays an important role in autoimmune disease by inducing interferon-γ secretion. Considering the abnormal serum concentration of IL-18 in primary immune thrombocytopenia (ITP) patients and the regulated effect of IL-18 gene polymorphisms on its production, the aim of this study was to investigate a possible association between the IL-18 promoter polymorphisms (-137 G/C and -607 C/A sites) and genetic susceptibility to ITP in a Chinese Han population. A total of 181 ITP patients and 163 healthy controls were included in this study; IL-18 gene promoter polymorphisms were analyzed by a polymerase chain reaction with sequence-specific primers. No significant differences in genotype (-607: χ(2)=0.307, p=0.858; -137: χ(2)=0.378, p=0.828) and allele frequencies (-607: χ(2)=0.004, p=0.949; -137: χ(2)=0.307, p=0.858) were found between total ITP patients and normal controls. We further analyzed the association of IL-18 polymorphisms with clinical parameters of ITP patients, including first onset age and clinical therapy response to glucocorticoids, and no difference was revealed. In conclusion, IL-18 promoter polymorphisms may not be associated with genetic susceptibility to ITP in a Chinese Han population.
authors.affiliation: State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College , Tianjin, People's Republic of China .
authors.forename: Huiyuan
authors.initials: H
authors.lastname: Li
authors.forename: Min
authors.initials: M
authors.lastname: Xuan
authors.forename: Feng
authors.initials: F
authors.lastname: Xue
authors.forename: Xiaofan
authors.initials: X
authors.lastname: Liu
authors.forename: Donglei
authors.initials: D
authors.lastname: Zhang
authors.forename: Rongfeng
authors.initials: R
authors.lastname: Fu
authors.forename: Yanhui
authors.initials: Y
authors.lastname: Yang
authors.forename: Xian
authors.initials: X
authors.lastname: Zhang
authors.forename: Renchi
authors.initials: R
authors.lastname: Yang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D002681:China; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D006801:Humans; D007223:Infant; D020382:Interleukin-18 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D016553:Purpura, Thrombocytopenic, Idiopathic / Q000235:genetics*; D012306:Risk; D017422:Sequence Analysis, DNA; D055815:Young Adult
chemical_list: C000589760:IL18 protein, human; D020382:Interleukin-18
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