title: The cytochrome c peroxidase and cytochrome c encounter complex: the other side of the story.
abstract: Formation of an encounter complex is important for efficient protein complex formation. The encounter state consists of an ensemble of orientations of two proteins in the complex. Experimental description of such ensembles inherently suffers from insufficient data availability. We have measured paramagnetic relaxation enhancements (PRE) on cytochrome c peroxidase (CcP) caused by its partner cytochrome c (Cc) carrying a spin label. The data complement earlier PRE data of spin labelled CcP, identifying several new interactions. This work demonstrates the need of obtaining as many independent data sets as possible to achieve the most accurate description of an encounter complex.
authors.affiliation: Leiden Institute of Chemistry, Gorlaeus Laboratories, Leiden University, Einsteinweg 55, 2333 CC Leiden, The Netherlands.
authors.forename: Jesika
authors.initials: J
authors.lastname: Schilder
authors.affiliation: Institute of Biophysical Chemistry, Goethe University Frankfurt and Centre for Biomolecular Magnetic Resonance, Max-von-Laue Strasse 9, 60438 Frankfurt am Main, Germany.
authors.forename: Frank
authors.initials: F
authors.lastname: Löhr
authors.affiliation: Institute of Organic Chemistry and Chemical Biology, Goethe University Frankfurt and Centre for Biomolecular Magnetic Resonance, Max-von-Laue Strasse 7, 60438 Frankfurt am Main, Germany.
authors.forename: Harald
authors.initials: H
authors.lastname: Schwalbe
authors.affiliation: Leiden Institute of Chemistry, Gorlaeus Laboratories, Leiden University, Einsteinweg 55, 2333 CC Leiden, The Netherlands. Electronic address: m.ubbink@chem.leidenuniv.nl.
authors.forename: Marcellus
authors.initials: M
authors.lastname: Ubbink
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites / Q000235:genetics; D003578:Cytochrome-c Peroxidase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D045304:Cytochromes c / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D007700:Kinetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D046912:Multiprotein Complexes / Q000737:chemistry* / Q000378:metabolism; D009154:Mutation; D019906:Nuclear Magnetic Resonance, Biomolecular; D011485:Protein Binding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D013113:Spin Labels
chemical_list: D046912:Multiprotein Complexes; D029701:Saccharomyces cerevisiae Proteins; D013113:Spin Labels; D045304:Cytochromes c; D003578:Cytochrome-c Peroxidase
keywords: Electron transfer; Paramagnetic NMR; Paramagnetic relaxation enhancement; Protein complex; Spin label; Transient complex