title: IL-6 upregulates a disintegrin and metalloproteinase with thrombospondin motifs 2 (ADAMTS-2) in human osteosarcoma cells mediated by JNK pathway.
abstract: ADAMTS-2 and ADAMTS-3 (a disintegrin and metalloproteinase with thrombospondin type 1 motif 2) belong to the procollagen aminoproteinase subfamily of ADAMTS proteases. They play crucial roles in the collagen metabolism. To understand the regulation of ADAMTS-2 gene expression in osteoblastic cells, we have cloned a functional 760 bp of human ADAMTS-2 promoter. Sequence analysis of the ADAMTS-2 promoter region showed the absence of a TATA box, but identified a GC box, a CpG island, several GAGA boxes and several transcriptional factor binding sites, which may be valuable in the regulation of ADAMTS-2 transcription. We also elucidated that Interleukin 6 (IL-6) increases ADAMTS-2 and ADAMTS-3 mRNA and protein levels in different osteosarcoma cell lines namely, MG-63 and Saos-2. IL-6 also increases the transcriptional activation of the ADAMTS-2 gene promoter. Pathway inhibition studies revealed that ADAMTS-2 upregulation by IL-6 was mediated by JNK pathway.
authors.affiliation: Department of Biology, Faculty of Science and Literature, Balıkesir University, Balıkesir, 10145, Turkey.
authors.forename: Meltem
authors.initials: M
authors.lastname: Alper
authors.forename: Feray
authors.initials: F
authors.lastname: Kockar
mesh_terms: D051722:ADAM Proteins / Q000096:biosynthesis* / Q000235:genetics; D000071096:ADAMTS Proteins; D000071121:ADAMTS4 Protein; D045744:Cell Line, Tumor; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis; D006801:Humans; D015850:Interleukin-6 / Q000235:genetics* / Q000378:metabolism; D020935:MAP Kinase Signaling System; D012516:Osteosarcoma / Q000235:genetics* / Q000473:pathology; D011348:Procollagen N-Endopeptidase / Q000096:biosynthesis* / Q000235:genetics; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000096:biosynthesis; D015533:Transcriptional Activation / Q000235:genetics*
chemical_list: D016326:Extracellular Matrix Proteins; D015850:Interleukin-6; D012333:RNA, Messenger; D051722:ADAM Proteins; D000071096:ADAMTS Proteins; C434951:ADAMTS3 protein, human; C000609307:ADAMTS2 protein, human; D011348:Procollagen N-Endopeptidase; D000071121:ADAMTS4 Protein
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