title: Purification of a polysaccharide from Gynostemma pentaphyllum Makino and its therapeutic advantages for psoriasis.
abstract: In current study, a water-soluble polysaccharide (GP-I), with a molecular mass of 33 kDa, was purified from Gynostemma pentaphyllum. Gas chromatography (GC) analysis suggested that it was composed of Glc, Gal, Man, Rha and Ara with a ratio of 5.3: 4.2: 3.0: 0.7: 0.8. The GP-I (25, 50, 100, 200 and 400 μg/ml) was found to have significant anti-proliferative effects on HaCat cells in a dose-dependent manner, as measured by MTT assay. On the contrary, Trypan blue exclusion experiment indicated that GP-I had no cytotoxicity to HaCat cells. Moreover, the decrease of mitochondrial membrane potential (MMP) in GP-I treated cells was also observed, indicating apoptosis in HaCat cells. Besides, tumor necrosis factor-α (TNF-α), a vital pro-inflammatory cytokine in psoriasis, in the supernatant of HaCat cells was dramatically reduced by GP-I. Collectively, these findings suggested that GP-I was a promising agent to be developed for psoriasis treatment in clinical therapy.
authors.affiliation: Department of Dermatology, The Second Hospital, Xi'an Jiaotong University, Xi'an 710004, China.
authors.forename: Xiao-Li
authors.initials: XL
authors.lastname: Li
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, The Second Hospital, Xi'an Jiaotong University, Xi'an 710004, China.
authors.forename: Zheng-Hui
authors.initials: ZH
authors.lastname: Wang
authors.affiliation: Department of Biological Science and Bioengineering, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China.
authors.forename: Yong-Xi
authors.initials: YX
authors.lastname: Zhao
authors.affiliation: Department of Dermatology, Tianjin Medical University General Hospital, Tianjin 300052, China.
authors.forename: Su-Ju
authors.initials: SJ
authors.lastname: Luo
authors.affiliation: Department of Dermatology, The Second Hospital, Xi'an Jiaotong University, Xi'an 710004, China.
authors.forename: Ding-Wei
authors.initials: DW
authors.lastname: Zhang
authors.affiliation: Department of Dermatology, The Second Hospital, Xi'an Jiaotong University, Xi'an 710004, China.
authors.forename: Sheng-Xiang
authors.initials: SX
authors.lastname: Xiao
authors.affiliation: Department of Dermatology, The Second Hospital, Xi'an Jiaotong University, Xi'an 710004, China.
authors.forename: Zhen-Hui
authors.initials: ZH
authors.lastname: Peng
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D029774:Gynostemma / Q000737:chemistry*; D006801:Humans; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects*; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D011565:Psoriasis / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D011134:Polysaccharides; D014409:Tumor Necrosis Factor-alpha