title: [Comparison of routine use of two chromogenic media ChromID CPS (bioMérieux) and UriSelect4 (Bio-Rad) for the detection of Escherichia coli and major uropathogenics in urine].
abstract: Escherichia coli is the most common bacterial cause of urinary tract infections. Its rapid and specific identification in urine samples represents a major challenge within the rendering results and optimizing the management of the patient. We aimed to compare the sensitivity and specificity of two commercially available chromogenic media for E. coli: ChromID CPS (Biomérieux) and UriSelect4 (Bio(-)Rad), without carrying out further tests. 99 consecutive and non-redundant urine samples considered to be infected were simultaneously plated onto blood agar and the two chromogenic media. Colony color and bacterial growth quantification were compared 18 and 48 hours after incubation. Bacteria were identified with mass spectrometry. A complementary analysis on 80 bacterial strains known to pose potential identification problems was performed. 43 urines samples grew E. coli, and 42 of them were pink-colored on the two chromogenic mediums, as expected (sensibility=97.7%). Growth quantification was significantly greater on blood agar than on chromogenic media (p<0.001).We noted specificity issues at the complementary analysis with the UriSelect4 medium: Citrobacter freundii and some strains of Citrobacter brakii, Enterobacter cloacae and Hafnia alvei were pink-colored, and could be misidentified as E. coli. ChromID CPS medium did not show such misidentification. In conclusion, the agar ChromID CPS proved to be greater than the UriSelect4 agar in our work in terms of specificity of direct identification of E. Coli, without the use of additional test.
authors.affiliation: Plateau technique de microbiologie, Laboratoire de bactériologie, CHU Strasbourg, France.
authors.forename: Mariam
authors.initials: M
authors.lastname: Meddeb
authors.affiliation: Plateau technique de microbiologie, Laboratoire de bactériologie, CHU Strasbourg, France.
authors.forename: Maxime
authors.initials: M
authors.lastname: Maurer
authors.affiliation: Plateau technique de microbiologie, Laboratoire de bactériologie, CHU Strasbourg, France.
authors.forename: Antoine
authors.initials: A
authors.lastname: Grillon
authors.affiliation: Plateau technique de microbiologie, Laboratoire de bactériologie, CHU Strasbourg, France.
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Scheftel
authors.affiliation: Plateau technique de microbiologie, Laboratoire de bactériologie, CHU Strasbourg, France.
authors.forename: Benoît
authors.initials: B
authors.lastname: Jaulhac
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods; D002863:Chromogenic Compounds / Q000737:chemistry / Q000494:pharmacology*; D003470:Culture Media / Q000737:chemistry*; D003955:Diagnostic Tests, Routine / Q000379:methods*; D004926:Escherichia coli / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000175:diagnosis* / Q000652:urine; D006801:Humans; D012680:Sensitivity and Specificity; D016482:Urinalysis / Q000379:methods*; D014552:Urinary Tract Infections / Q000175:diagnosis* / Q000382:microbiology / Q000652:urine; D056689:Uropathogenic Escherichia coli / Q000302:isolation & purification*
chemical_list: D002863:Chromogenic Compounds; D003470:Culture Media
keywords: Escherichia coli; chromogenic media; specificity