title: Clinical success of intrauterine insemination cycles is affected by the sperm preparation time.
abstract: OBJECTIVE
  To determine the impact of the time interval from the end of sperm preparation (TSP) to intrauterine insemination (IUI) on the outcome.
  
  
  METHODS
  Prospective multicentre cohort study.
  
  
  METHODS
  Seven French centers (assisted reproduction group in northeastern France, four academic centers, and three clinics).
  
  
  METHODS
  Eight hundred sixty-two IUI cycles (709 patients) managed by gonadotropins were studied.
  
  
  METHODS
  Cycles were stimulated by either FSH or hMG, and hCG was administrated when the leading follicle diameter measured >15 mm. IUIs were performed ∼ 36 hours after ovulation triggering.
  
  
  METHODS
  Generalized linear mixed models for binary outcomes were used to model clinical pregnancy (CP) to assess the effect of TSP adjusted for other predictors (such as maternal age, semen quality, and indication of IUI treatment).
  
  
  RESULTS
  The TSP effect was significant, featuring an inverse U-shaped curve admitting an optimum interval of ∼ 40-80 minutes improving CP compared with other values. Other significant predictors were total motile spermatozoa inseminated, maternal age, and unexplained infertility.
  
  
  CONCLUSIONS
  The observance of TSP in the range of 40-80 minutes has a potential positive effect on pregnancy rate, while not requiring the investment of supplemental resources. This finding awaits confirmation in randomized trials.
authors.affiliation: Laboratoire de Biologie de la Reproduction, Hôpital de Dijon, Université de Bourgogne, Dijon, France. Electronic address: patricia.fauque@chu-dijon.fr.
authors.forename: Patricia
authors.initials: P
authors.lastname: Fauque
authors.affiliation: Faculty of Medicine, University of Melbourne, Victoria, Australia, and Faculty of Economics, Louvain University, Louvain-la-Neuve, Belgium.
authors.forename: Philippe
authors.initials: P
authors.lastname: Lehert
authors.affiliation: Médecine Fœtale et Stérilité Conjugale, Service de Gynécologie-Obstétrique, Hôpital de Dijon, Université de Bourgogne, Dijon, France.
authors.forename: Marjorie
authors.initials: M
authors.lastname: Lamotte
authors.affiliation: Centre d'Assistance Médicale à la Procréation, CMCO-SIHCUS, Schiltigheim, France.
authors.forename: Karima
authors.initials: K
authors.lastname: Bettahar-Lebugle
authors.affiliation: Laboratoire de Biologie de la Reproduction, Université de Franche-Comté, Hôpital de Besançon, Besançon, France.
authors.forename: Alphée
authors.initials: A
authors.lastname: Bailly
authors.affiliation: Unité de Biologie de la Reproduction, Université de Lorraine, Maternité Régionale Universitaire de Nancy, Nancy, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Diligent
authors.affiliation: Polyclinique Majorelle, Nancy, France.
authors.forename: Michel
authors.initials: M
authors.lastname: Clédat
authors.affiliation: Centre FIV de Haute Alsace, Mulhouse, France.
authors.forename: Paul
authors.initials: P
authors.lastname: Pierrot
authors.affiliation: Laboratoire du Vignoble, Kaysersberg, France.
authors.forename: Marie-Lorraine
authors.initials: ML
authors.lastname: Guénédal
authors.affiliation: Médecine Fœtale et Stérilité Conjugale, Service de Gynécologie-Obstétrique, Hôpital de Dijon, Université de Bourgogne, Dijon, France.
authors.forename: Paul
authors.initials: P
authors.lastname: Sagot
mesh_terms: D000328:Adult; D000367:Age Factors; D005260:Female; D005298:Fertility; D005300:Fertility Agents, Female / Q000008:administration & dosage; D005602:France; D006801:Humans; D007246:Infertility / Q000209:etiology / Q000503:physiopathology / Q000628:therapy*; D007317:Insemination, Artificial, Homologous; D016014:Linear Models; D008297:Male; D015999:Multivariate Analysis; D016017:Odds Ratio; D010062:Ovulation Induction; D011247:Pregnancy; D018873:Pregnancy Rate; D011446:Prospective Studies; D055101:Semen Analysis; D013048:Specimen Handling / Q000379:methods*; D013094:Spermatozoa / Q000473:pathology*; D013997:Time Factors; D014019:Tissue Donors; D016896:Treatment Outcome
chemical_list: D005300:Fertility Agents, Female
keywords: Intrauterine insemination; pregnancy; sperm process; timing