title: Aneurysmal lesions of patients with abdominal aortic aneurysm contain clonally expanded T cells.
abstract: Abdominal aortic aneurysm (AAA) is a common disease with often life-threatening consequences. This vascular disorder is responsible for 1-2% of all deaths in men aged 65 years or older. Autoimmunity may be responsible for the pathogenesis of AAA. Although it is well documented that infiltrating T cells are essentially always present in AAA lesions, little is known about their role in the initiation and/or progression of the disease. To determine whether T cells infiltrating AAA lesions contain clonally expanded populations of T cells, we amplified β-chain TCR transcripts by the nonpalindromic adaptor-PCR/Vβ-specific PCR and/or Vβ-specific PCR, followed by cloning and sequencing. We report in this article that aortic abdominal aneurysmal lesions from 8 of 10 patients with AAA contained oligoclonal populations of T cells. Multiple identical copies of β-chain TCR transcripts were identified in these patients. These clonal expansions are statistically significant. These results demonstrate that αβ TCR(+) T lymphocytes infiltrating aneurysmal lesions of patients with AAA have undergone proliferation and clonal expansion in vivo at the site of the aneurysmal lesion, in response to unidentified self- or nonself Ags. This evidence supports the hypothesis that AAA is a specific Ag-driven T cell disease.
authors.affiliation: Department of Microbiology and Immunology, Temple University School of Medicine, Philadelphia, PA 19140;
authors.forename: Song
authors.initials: S
authors.lastname: Lu
authors.forename: John V
authors.initials: JV
authors.lastname: White
authors.forename: Wan Lu
authors.initials: WL
authors.lastname: Lin
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Zhang
authors.forename: Charalambos
authors.initials: C
authors.lastname: Solomides
authors.forename: Kyle
authors.initials: K
authors.lastname: Evans
authors.forename: Nectaria
authors.initials: N
authors.lastname: Ntaoula
authors.forename: Ifeyinwa
authors.initials: I
authors.lastname: Nwaneshiudu
authors.forename: John
authors.initials: J
authors.lastname: Gaughan
authors.forename: Dimitri S
authors.initials: DS
authors.lastname: Monos
authors.forename: Emilia L
authors.initials: EL
authors.lastname: Oleszak
authors.forename: Chris D
authors.initials: CD
authors.lastname: Platsoucas
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D017544:Aortic Aneurysm, Abdominal / Q000276:immunology* / Q000473:pathology; D049109:Cell Proliferation; D060966:Clonal Selection, Antigen-Mediated; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology* / Q000473:pathology
chemical_list: D016693:Receptors, Antigen, T-Cell, alpha-beta
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