title: Occult hepatitis B virus infection predicts de novo hepatitis B infection in patients with alcoholic cirrhosis after liver transplantation.
abstract: OBJECTIVE
  Occult hepatitis B virus infection (OBI) in patients undergoing liver transplantation (LT) is a suspected source of de novo hepatitis B virus (HBV) infection after LT. This study aimed to investigate the prevalence of OBI in liver transplant recipients with alcoholic cirrhosis and demonstrate the association between OBI and de novo HBV infection after LT in these patients.
  
  
  METHODS
  Forty-three patients with alcoholic cirrhosis who were negative for HBsAg before LT were recruited in this retrospective study. DNA was extracted from paraffin-embedded native liver tissues and quantified for HBV DNA by real-time PCR. Correlation between de novo HBV infection after LT (positive HBsAg and/or detectable HBV DNA in serum) and detection of intrahepatic HBV DNA before LT was analysed.
  
  
  RESULTS
  Detectable HBV DNA in the explanted liver was found in 41.9% (18/43) of the patients and was thus defined as OBI, which was correlated with the presence of serum hepatitis B core antibody (P = 0.008). De novo HBV infection occurred in 18.6% (8/43) of the recipients at a median of 10 months after LT. The rate of de novo HBV infection was 38.9% (7/18) in patients with OBI, compared with 4% (1/25) in patients without OBI (P = 0.004). Furthermore, de novo HBV infection was inversely correlated with the presence of hepatitis B surface antibody in recipients with OBI (P = 0.026).
  
  
  CONCLUSIONS
  With a prevalence of 41.9% in liver transplant recipients with alcoholic cirrhosis, OBI in the native liver can predict de novo HBV infection after LT.
authors.affiliation: Liver Research Center, Beijing Friendship Hospital, Capital Medical University, Beijing, 100050, China; Beijing Key Laboratory of Translational Medicine in Liver Cirrhosis, Beijing, 100050, China.
authors.forename: Man
authors.initials: M
authors.lastname: Xie
authors.forename: Wei
authors.initials: W
authors.lastname: Rao
authors.forename: Tao
authors.initials: T
authors.lastname: Yang
authors.forename: Yonglin
authors.initials: Y
authors.lastname: Deng
authors.forename: Hong
authors.initials: H
authors.lastname: Zheng
authors.forename: Cheng
authors.initials: C
authors.lastname: Pan
authors.forename: Yihe
authors.initials: Y
authors.lastname: Liu
authors.forename: Zhongyang
authors.initials: Z
authors.lastname: Shen
authors.forename: Jidong
authors.initials: J
authors.lastname: Jia
mesh_terms: D000328:Adult; D002681:China / Q000453:epidemiology; D058625:End Stage Liver Disease / Q000601:surgery / Q000821:virology*; D005260:Female; D006509:Hepatitis B / Q000150:complications* / Q000453:epidemiology; D006801:Humans; D008104:Liver Cirrhosis, Alcoholic / Q000601:surgery / Q000821:virology*; D016031:Liver Transplantation; D008297:Male; D008875:Middle Aged; D011183:Postoperative Complications / Q000821:virology*; D015995:Prevalence; D012189:Retrospective Studies; D012307:Risk Factors
keywords: alcohol; cirrhosis; de novo HBV infection; hepatitis B virus infection; orthotopic liver transplantation