title: Detection of glycoalkaloids using disposable biosensors based on genetically modified enzymes.
abstract: In this work we present a rapid, selective, and highly sensitive detection of α-solanine and α-chaconine using cholinesterase-based sensors. The high sensitivity of the devices is brought by the use of a genetically modified acetylcholinesterase (AChE), combined with a one-step detection method based on the measurement of inhibition slope. The selectivity was obtained by using butyrylcholinesterase (BChE), an enzyme able to detect these two toxins with differential inhibition kinetics. The enzymes were immobilized via entrapment in PVA-AWP polymer directly on the working electrode surface. The analysis of the resulting inhibition slope was performed employing linear regression function included in Matlab. The high toxicity of α-chaconine compared to α-solanine due to a better affinity to the active site was proved. The inhibition of glycoalkaloids (GAs) mixture was performed over AChE enzyme wild-type AChE and BChE biosensors resulting in the detection of synergism effect. The developed method allows the detection of (GAs) at 50 ppb in potato matrix.
authors.affiliation: Instituto de Ingeniería, Universidad Autónoma de Baja California, California Calle de la Normal S/N, Blvd. Benito Juárez, CP 211000 Mexicali, B.C., Mexico. Electronic address: Michelle.arredondo@uabc.edu.mx.
authors.forename: Michelle Arredondo
authors.initials: MA
authors.lastname: Espinoza
authors.affiliation: Université de Perpignan Via Domitia, IMAGES EA4218, Building S, 52 Avenue Paul Alduy, 66860 Perpignan Cedex, France. Electronic address: gistamboulie@yahoo.fr.
authors.forename: Georges
authors.initials: G
authors.lastname: Istamboulie
authors.affiliation: National Institute of Research and Development for Biological Sciences, Centre of Bioanalysis, 296, Splaiul Independentei, Bucharest 060031, Romania. Electronic address: ana_chira@yahoo.com.
authors.forename: Ana
authors.initials: A
authors.lastname: Chira
authors.affiliation: Université de Perpignan Via Domitia, IMAGES EA4218, Building S, 52 Avenue Paul Alduy, 66860 Perpignan Cedex, France. Electronic address: noguer@univ-perp.fr.
authors.forename: Thierry
authors.initials: T
authors.lastname: Noguer
authors.affiliation: Instituto de Ingeniería, Universidad Autónoma de Baja California, California Calle de la Normal S/N, Blvd. Benito Juárez, CP 211000 Mexicali, B.C., Mexico. Electronic address: margarita@uabc.edu.mx.
authors.forename: Margarita
authors.initials: M
authors.lastname: Stoytcheva
authors.affiliation: Université de Perpignan Via Domitia, IMAGES EA4218, Building S, 52 Avenue Paul Alduy, 66860 Perpignan Cedex, France. Electronic address: jlmarty@univ-perp.fr.
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Marty
mesh_terms: D000110:Acetylcholinesterase / Q000235:genetics / Q000378:metabolism*; D015374:Biosensing Techniques / Q000295:instrumentation*; D002091:Butyrylcholinesterase / Q000235:genetics / Q000378:metabolism*; D004209:Disposable Equipment; D005818:Genetic Engineering; D012992:Solanine / Q000031:analogs & derivatives* / Q000737:chemistry; D011198:Solanum tuberosum / Q000737:chemistry
chemical_list: C514919:alpha-solanine; D012992:Solanine; C011860:alpha-chaconine; D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase
keywords: ANNs; Acetylcholinesterase; Biosensors; Butyrylcholinesterase; Glycoalkaloids