title: Genotoxicity and cytotoxicity evaluation of oenothein B and its protective effect against mitomycin C-induced mutagenic action.
abstract: The natural product oenothein B (OeB), a dimeric macrocyclic ellagitannin, has a wide range of biological activities, such as antioxidant, anti-inflammatory, anti-viral, antifungal, and antitumor. However, investigations concerning its genotoxicity have not been carried out. This study assessed the cytotoxicity, genotoxicity, and protective effects of oenothein B using in vitro SOS-Inductest and in vivo mouse bone marrow micronucleus (MN) assay through oral and intraperitonial routes. In both assays oenothein B did not produce genotoxic effects in any of doses tested; in contrast, cytotoxic effect in cells was detected only in mice groups treated by both routes and exposed for 24 and 48h. Antigenotoxic and anticytotoxic activities of oenothein B were evaluated using both assays in combination with mitomycin C (MMC), a bioreductive alkylating agent. In the MN assay, a significant reduction was observed in MN frequency in all groups co-treated with MMC and OeB compared to those which received only MMC. Anticytotoxicity was observed in mice groups exposed to OeB and MMC for 24 and 48h. In the SOS-Inductest, oenothein B failed to show antigenotoxic and anticytotoxic effects; thus, it undoubtedly showed an in vivo protective activity against primary DNA damage induced by mitomycin C.
authors.affiliation: Institute of Biological Sciences, Federal University of Goiás, 74001-970 Goiânia, GO, Brazil.
authors.forename: Cinthia Aparecida
authors.initials: CA
authors.lastname: Silva
authors.affiliation: Institute of Biological Sciences, Federal University of Goiás, 74001-970 Goiânia, GO, Brazil.
authors.forename: Carolina Ribeiro
authors.initials: CR
authors.lastname: Silva
authors.affiliation: Institute of Biological Sciences, Federal University of Goiás, 74001-970 Goiânia, GO, Brazil.
authors.forename: Jefferson Hollanda
authors.initials: JH
authors.lastname: Véras
authors.affiliation: Institute of Biological Sciences, Federal University of Goiás, 74001-970 Goiânia, GO, Brazil.
authors.forename: Lee
authors.initials: L
authors.lastname: Chen-Chen
authors.affiliation: Institute of Chemistry, Federal University of Goiás, 74001-970 Goiânia, GO, Brazil.
authors.forename: Pedro Henrique
authors.initials: PH
authors.lastname: Ferri
authors.affiliation: Institute of Chemistry, Federal University of Goiás, 74001-970 Goiânia, GO, Brazil. Electronic address: suzana.quimica.ufg@hotmail.com.
authors.forename: Suzana da Costa
authors.initials: Sda C
authors.lastname: Santos
mesh_terms: D000477:Alkylating Agents / Q000494:pharmacology*; D000818:Animals; D003603:Cytotoxins / Q000009:adverse effects / Q000494:pharmacology*; D004249:DNA Damage; D004926:Escherichia coli; D047348:Hydrolyzable Tannins / Q000009:adverse effects / Q000494:pharmacology*; D051379:Mice; D016685:Mitomycin / Q000494:pharmacology*; D013014:SOS Response, Genetics / Q000187:drug effects*
chemical_list: D000477:Alkylating Agents; D003603:Cytotoxins; D047348:Hydrolyzable Tannins; C080077:oenothein B; D016685:Mitomycin
keywords: Antigenotoxicity; Ellagitannin; Micronucleous assay; Mitomycin C; Oenothein B; SOS-Inductest