title: Changes in the serum metabolite profile in obese children with weight loss.
abstract: OBJECTIVE
  Childhood obesity is an increasing problem and is accompanied by metabolic disturbances. Recently, we have identified 14 serum metabolites by a metabolomics approach (FIA-MS/MS), which showed altered concentrations in obese children as compared to normal-weight children. Obese children demonstrated higher concentrations of two acylcarnitines and lower levels of three amino acids, six acyl-alkyl phosphatidylcholines, and three lysophosphatidylcholines. The aim of this study was to analyze whether these alterations normalize in weight loss.
  
  
  METHODS
  We analyzed the changes of these 14 metabolites by the same metabolic kit as in our previous study in serum samples of 80 obese children with substantial weight loss (BMI-SDS reduction >0.5) and in 80 obese children with stable weight status all participating in a 1-year lifestyle intervention.
  
  
  RESULTS
  In the children without weight change, no significant changes of metabolite concentrations could be observed. In children with substantial weight loss, glutamine, methionine, the lysophosphatidylcholines LPCaC18:1, LPCaC18:2, and LPCa20:4, as well as the acyl-alkyl phosphatidylcholine PCaeC36:2 increased significantly, while the acylcarnitines C12:1 and C16:1, proline, PCaeC34:1, PCaeC34:2, PCaeC34:3, PCaeC36:3, and PCaeC38:2 did not change significantly.
  
  
  CONCLUSIONS
  The changes of glutamine, methionine, LPCaC18:1, LPCaC18:2, LPCa20:4, and PCaeC36:2 seem to be related to the changes of dieting or exercise habits in lifestyle intervention or to be a consequence of overweight since they normalized in weight loss. Further studies should substantiate our findings.
authors.affiliation: Department of Pediatric Endocrinology, Diabetes, and Nutrition Medicine, Vestische Hospital for Children and Adolescents, University of Witten/Herdecke, Dr. F. Steiner Str. 5, 45711, Datteln, Germany, T.Reinehr@kinderklinik-datteln.de.
authors.forename: Thomas
authors.initials: T
authors.lastname: Reinehr
authors.forename: Barbara
authors.initials: B
authors.lastname: Wolters
authors.forename: Caroline
authors.initials: C
authors.lastname: Knop
authors.forename: Nina
authors.initials: N
authors.lastname: Lass
authors.forename: Christian
authors.initials: C
authors.lastname: Hellmuth
authors.forename: Ulrike
authors.initials: U
authors.lastname: Harder
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Peissner
authors.forename: Simone
authors.initials: S
authors.lastname: Wahl
authors.forename: Harald
authors.initials: H
authors.lastname: Grallert
authors.forename: Jerzy
authors.initials: J
authors.lastname: Adamski
authors.forename: Thomas
authors.initials: T
authors.lastname: Illig
authors.forename: Cornelia
authors.initials: C
authors.lastname: Prehn
authors.forename: Zhonghao
authors.initials: Z
authors.lastname: Yu
authors.forename: Rui
authors.initials: R
authors.lastname: Wang-Sattler
authors.forename: Berthold
authors.initials: B
authors.lastname: Koletzko
mesh_terms: D000293:Adolescent; D017195:Adolescent Nutritional Physiological Phenomena; D015992:Body Mass Index; D002648:Child; D002664:Child Nutritional Physiological Phenomena; D015331:Cohort Studies; D003131:Combined Modality Therapy; D004038:Diet, Reducing; D015536:Down-Regulation; D015444:Exercise; D005260:Female; D005858:Germany; D005973:Glutamine / Q000097:blood* / Q000378:metabolism; D006801:Humans; D008019:Life Style; D008137:Longitudinal Studies; D008244:Lysophosphatidylcholines / Q000097:blood* / Q000378:metabolism; D008297:Male; D008715:Methionine / Q000097:blood* / Q000378:metabolism; D009765:Obesity / Q000097:blood / Q000178:diet therapy / Q000378:metabolism / Q000628:therapy*; D010742:Phospholipid Ethers / Q000097:blood* / Q000378:metabolism; D015431:Weight Loss
chemical_list: D008244:Lysophosphatidylcholines; D010742:Phospholipid Ethers; D005973:Glutamine; D008715:Methionine
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