title: Surface-induced conformational changes in doped bovine serum albumin self-assembled monolayers.
abstract: Evidence for considerable stabilization of doped bovine serum albumin (BSA) molecules upon adsorption on gold surfaces is provided. This is compared to the surface-induced conformational changes of the bare BSA and its corresponding monolayer. The BSA unfolding phenomenon is correlated with dehydration, which in turn enables improved monolayer coverage. The stabilization mechanism is found to be partially controllable via nanodoping of the BSA molecules, upon which the dehydration process is suppressed and molecular rigidity can be varied. Our experimental data and calculations further point to the intermixing of structural characteristics and inherent molecular properties in studies of biological monolayers.
authors.affiliation: University Center for Nanoscience and Nanotechnology, ‡School of Chemistry, Raymond and Beverly Sackler Faculty of Exact Sciences, and §Department of Materials Science and Engineering, Tel Aviv University , Tel-Aviv, Israel 6997801.
authors.forename: Edith
authors.initials: E
authors.lastname: Beilis
authors.forename: Bogdan
authors.initials: B
authors.lastname: Belgorodsky
authors.forename: Ludmila
authors.initials: L
authors.lastname: Fadeev
authors.forename: Hagai
authors.initials: H
authors.lastname: Cohen
authors.forename: Shachar
authors.initials: S
authors.lastname: Richter
mesh_terms: D000327:Adsorption; D000818:Animals; D002417:Cattle; D006046:Gold / Q000737:chemistry*; D008958:Models, Molecular; D055550:Protein Stability; D017433:Protein Structure, Secondary; D058767:Protein Unfolding; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D014867:Water / Q000737:chemistry
chemical_list: D014867:Water; D012710:Serum Albumin, Bovine; D006046:Gold