title: MicroRNAs involving in cold, wounding and salt stresses in Triticum aestivum L.
abstract: MicroRNAs (miRNAs) play critical roles in post-transcriptional regulation and act as important endogenous regulators to various stresses. Cold, wounding and high-salinity are three common environmental stress stimuli influencing crops growth and development. In this study, we identified 31 known miRNAs and 3 novel miRNAs in wheat. Moreover, 19 stress-regulated miRNAs using RT-qPCR data in which the effects of three stresses were surveyed from the known miRNAs. Among them, 16, 12 and 8 miRNAs were regulated under cold, wounding and high-salinity treatments, respectively. Of which 4 miRNAs were highly responsive to cold stress in wheat by northern blot, and 6 wounding-regulated and 3 high-salinity-regulated miRNAs were detected. Meanwhile, miR159, miR393 and miR398 were responsive to multiple stress stimuli. Besides, 2 novel miRNAs were regulated by cold stress. While, the analyses of targets suggested miR159, miR398 and miR6001 could responses to stress conditions in regulation pathways. Taken together, the results of this study suggest that wheat miRNAs may play important roles in response to abiotic stress.
authors.affiliation: College of Plant Protection and State Key Laboratory of Crop Stress Biology on Drought Regions, Northwest A&F University, No. 3 Taicheng Road, Yangling, Shaanxi 712100, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Wang
authors.affiliation: College of Plant Protection and State Key Laboratory of Crop Stress Biology on Drought Regions, Northwest A&F University, No. 3 Taicheng Road, Yangling, Shaanxi 712100, China.
authors.forename: Yan-Fei
authors.initials: YF
authors.lastname: Sun
authors.affiliation: College of Plant Protection and State Key Laboratory of Crop Stress Biology on Drought Regions, Northwest A&F University, No. 3 Taicheng Road, Yangling, Shaanxi 712100, China.
authors.forename: Na
authors.initials: N
authors.lastname: Song
authors.affiliation: College of Plant Protection and State Key Laboratory of Crop Stress Biology on Drought Regions, Northwest A&F University, No. 3 Taicheng Road, Yangling, Shaanxi 712100, China.
authors.forename: Jin-Ping
authors.initials: JP
authors.lastname: Wei
authors.affiliation: College of Plant Protection and State Key Laboratory of Crop Stress Biology on Drought Regions, Northwest A&F University, No. 3 Taicheng Road, Yangling, Shaanxi 712100, China.
authors.forename: Xiao-Jie
authors.initials: XJ
authors.lastname: Wang
authors.affiliation: College of Plant Protection and State Key Laboratory of Crop Stress Biology on Drought Regions, Northwest A&F University, No. 3 Taicheng Road, Yangling, Shaanxi 712100, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Feng
authors.affiliation: College of Plant Protection and State Key Laboratory of Crop Stress Biology on Drought Regions, Northwest A&F University, No. 3 Taicheng Road, Yangling, Shaanxi 712100, China.
authors.forename: Zhi-Yuan
authors.initials: ZY
authors.lastname: Yin
authors.affiliation: College of Plant Protection and State Key Laboratory of Crop Stress Biology on Drought Regions, Northwest A&F University, No. 3 Taicheng Road, Yangling, Shaanxi 712100, China. Electronic address: kangzs@nwsuaf.edu.cn.
authors.forename: Zhen-Sheng
authors.initials: ZS
authors.lastname: Kang
mesh_terms: D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000235:genetics; D035683:MicroRNAs / Q000235:genetics*; D018749:RNA, Plant / Q000235:genetics*; D012965:Sodium Chloride / Q000494:pharmacology*; D014908:Triticum / Q000187:drug effects / Q000235:genetics*
chemical_list: D035683:MicroRNAs; D018749:RNA, Plant; D012965:Sodium Chloride
keywords: MiRNAs; Northern blot; Stress; Target; Wheat