title: Cycloartane Triterpenoids from Euphorbia Macrostegia with their Cytotoxicity against MDA-MB48 and MCF-7 Cancer Cell Lines.
abstract: The dried plant was extracted with dichloromethane and after defatting with hexane, transferred repeatedly on silica columns using dichloromethane-hexane and ethyl acetate-hexane as mobile phases. Finally the fractions were purified by high performance liquid chromatography using a Pack-Sil column and hexane: Ethyl acetate as mobile phase. The structures of the isolated compounds included: cycloart-25-ene-3β, 24-diol (1), cycloart-23(Z)-ene-3β, 25-diol (2), cycloart-23(E)-ene-3β, 25-diol (3), and 24-methylene-cycloart-3β-ol (4) were elucidated by (13)C- and (1)H-NMR as well as IR and by the aid of mass fragmentation pattern and comparing with the literature. The biological effects of the compounds were done by the MTT assay on two different cancer cell lines including MDA-MB48 and MCF-7. Among these compounds, cycloart-23(E)-ene-3β,25-diol (3) was the most active compound on MDA-MB468 cell line (LD50 = 2.05 μgmL(- 1) ) and cycloart-23(Z)-ene-3β, 25-diol (2) was the most active compound on MCF-7 cell line (LD50 = 5.4 μgmL(- 1)).
authors.affiliation: Isfahan Pharmaceutical Sciences Research Center, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.
authors.forename: Somayeh
authors.initials: S
authors.lastname: Baniadam
authors.affiliation: Department of Biology, Faculty of Science, University of Isfahan, Isfahan, I.R. Iran.
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Rahiminejad
authors.affiliation: Department of Pharmacognosy, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Ghannadian
authors.affiliation: Department of Biology, Faculty of Science, University of Isfahan, Isfahan, I.R. Iran.
authors.forename: Hojjatollah
authors.initials: H
authors.lastname: Saeidi
authors.affiliation: Phytochemistry Research Center,Department of Pharmacognosy, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Abdul Majid
authors.initials: AM
authors.lastname: Ayatollahi
authors.affiliation: Department of Clinical Biochemistry, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Aghaei
keywords: Cycloartane; Cytotoxicity; Euphorboa macrostegia; MCF-7; MDA-MB468
references.citation: Pharmacognosy Res. 2010 May;2(3):159-62
references.pmid: 21808559
references.citation: Iran J Pharm Res. 2013 Fall;12(4):695-703
references.pmid: 24523749
references.citation: Fitoterapia. 2013 Dec;91:87-94
references.pmid: 23994233
references.citation: Res Pharm Sci. 2013 Jul;8(3):205-10
references.pmid: 24019830
references.citation: J Org Chem. 2007 Jun 8;72(12):4578-81
references.pmid: 17506582
references.citation: Iran J Pharm Res. 2011 Winter;10(1):105-12
references.pmid: 24363688
references.citation: Phytochemistry. 2000 Apr;53(8):947-50
references.pmid: 10820809
references.citation: Iran J Pharm Res. 2013 Fall;12(4):789-99
references.pmid: 24523759
references.citation: Bioorg Med Chem. 2006 Feb 15;14(4):938-43
references.pmid: 16202612
references.citation: Anticancer Res. 2004 Mar-Apr;24(2B):859-64
references.pmid: 15161038
references.citation: J Ethnopharmacol. 2012 Jan 31;139(2):574-81
references.pmid: 22155389
references.citation: Eur J Pharmacol. 2013 Jan 5;698(1-3):470-9
references.pmid: 23117087
references.citation: J Nat Prod. 2012 Apr 27;75(4):599-604
references.pmid: 22360639
references.citation: Phytochemistry. 2006 Sep;67(18):1977-84
references.pmid: 16889806