title: First proposed panels on acute leukemia for four-color immunophenotyping by flow cytometry from the Brazilian group of flow cytometry-GBCFLUX.
abstract: Multiparameter flow cytometry is a highly sensitive, fast, and specific diagnostic technology with a wide range of applicability in hematology. Although well-established eight-color immunophenotyping panels are already available, most Brazilian clinical laboratories are equipped with four-color flow cytometer facilities. Based on this fact, the Brazilian Group of Flow Cytometry (Grupo Brasileiro de Citometria de Fluxo, GBCFLUX) for standardization of clinical flow cytometry has proposed an antibody panel designed to allow precise diagnosis and characterization of acute leukemia (AL) within resource-restricted areas. Morphological analysis of bone marrow smears, together with the screening panel, is mandatory for the primary identification of AL. The disease-oriented panels proposed here are divided into three levels of recommendations (mandatory, recommendable, and optional) in order to provide an accurate final diagnosis, as well as allow some degree of flexibility based on available local resources and patient-specific needs. The proposed panels will be subsequently validated in an interlaboratory study to evaluate its effectiveness on the diagnosis and classification of AL. (Assoc editor comm. 2).
authors.affiliation: Hospital Amaral Carvalho, Laboratório de Citometria de Fluxo do Hemonúcleo Regional de Jau, São Paulo, Brazil.
authors.forename: Maura R V
authors.initials: MR
authors.lastname: Ikoma
authors.forename: Alex F
authors.initials: AF
authors.lastname: Sandes
authors.forename: Leandro S
authors.initials: LS
authors.lastname: Thiago
authors.forename: Geraldo B
authors.initials: GB
authors.lastname: Cavalcanti Júnior
authors.forename: Irene G H
authors.initials: IG
authors.lastname: Lorand-Metze
authors.forename: Elaine S
authors.initials: ES
authors.lastname: Costa
authors.forename: Glicinia
authors.initials: G
authors.lastname: Pimenta
authors.forename: Maria C
authors.initials: MC
authors.lastname: Santos-Silva
authors.forename: Nydia S
authors.initials: NS
authors.lastname: Bacal
authors.forename: Mihoko
authors.initials: M
authors.lastname: Yamamoto
authors.forename: Elizabeth X
authors.initials: EX
authors.lastname: Souto
mesh_terms: D000906:Antibodies / Q000737:chemistry; D015703:Antigens, CD / Q000235:genetics / Q000276:immunology; D014408:Biomarkers, Tumor / Q000235:genetics / Q000276:immunology*; D001938:Brazil; D003116:Color; D020732:Cytogenetic Analysis; D005434:Flow Cytometry / Q000379:methods / Q000592:standards*; D005456:Fluorescent Dyes; D006801:Humans; D016130:Immunophenotyping / Q000379:methods / Q000592:standards*; D015470:Leukemia, Myeloid, Acute / Q000175:diagnosis* / Q000235:genetics / Q000276:immunology / Q000473:pathology; D008214:Lymphocytes / Q000145:classification / Q000276:immunology* / Q000473:pathology; D022423:Myeloid Cells / Q000145:classification / Q000276:immunology* / Q000473:pathology; D017410:Practice Guidelines as Topic; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000175:diagnosis* / Q000235:genetics / Q000276:immunology / Q000473:pathology
chemical_list: D000906:Antibodies; D015703:Antigens, CD; D014408:Biomarkers, Tumor; D005456:Fluorescent Dyes
keywords: GBCFLUX; acute leukemia panel; acute lymphoblastic leukemia; acute myeloblastic leukemia; flow cytometry