title: SIR-2.1 integrates metabolic homeostasis with the reproductive neuromuscular excitability in early aging male Caenorhabditis elegans.
abstract: The decline of aging C. elegans male's mating behavior is correlated with the increased excitability of the cholinergic circuitry that executes copulation. In this study, we show that the mating circuits' functional durability depends on the metabolic regulator SIR-2.1, a NAD(+)-dependent histone deacetylase. Aging sir-2.1(0) males display accelerated mating behavior decline due to premature hyperexcitability of cholinergic circuits used for intromission and ejaculation. In sir-2.1(0) males, the hypercontraction of the spicule-associated muscles pinch the vas deferens opening, thus blocking sperm release. The hyperexcitability is aggravated by reactive oxygen species (ROS). Our genetic, pharmacological, and behavioral analyses suggest that in sir-2.1(0) and older wild-type males, enhanced catabolic enzymes expression, coupled with the reduced expression of ROS-scavengers contribute to the behavioral decline. However, as a compensatory response to reduce altered catabolism/ROS production, anabolic enzymes expression levels are also increased, resulting in higher gluconeogenesis and lipid synthesis. DOI: http://dx.doi.org/10.7554/eLife.01730.001.
authors.affiliation: Department of Biology, Texas A&M University, College Station, United States.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Guo
authors.forename: L René
authors.initials: LR
authors.lastname: García
mesh_terms: D000375:Aging; D000818:Animals; D017173:Caenorhabditis elegans / Q000378:metabolism / Q000502:physiology*; D029742:Caenorhabditis elegans Proteins / Q000378:metabolism*; D004734:Energy Metabolism; D006655:Histone Deacetylases / Q000378:metabolism; D006706:Homeostasis; D008297:Male; D012726:Sexual Behavior, Animal; D037761:Sirtuins / Q000378:metabolism*
chemical_list: D029742:Caenorhabditis elegans Proteins; C507014:SIR-2.1 protein, C elegans; D037761:Sirtuins; D006655:Histone Deacetylases
keywords: PEPCK; behavioral aging; cell excitability; compensatory mechanism; metabolism; reactive oxygen species (ROS)
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