title: Inhibition controls for qualitative real-time PCR assays: are they necessary for all specimen matrices?
abstract: A retrospective analysis of 386,706 specimens representing a variety of matrix types used in qualitative real-time PCR assays determined the overall inhibition rate to be 0.87% when the inhibition control was added preextraction to 5,613 specimens and 0.01% when the inhibition control was added postextraction but preamplification in 381,093 specimens. Inhibition rates of ≤ 1% were found for all specimen matrix types except urine and formalin-fixed, paraffin-embedded tissue.
authors.affiliation: Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota, USA.
authors.forename: S P
authors.initials: SP
authors.lastname: Buckwalter
authors.affiliation: Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota, USA.
authors.forename: L M
authors.initials: LM
authors.lastname: Sloan
authors.affiliation: Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota, USA.
authors.forename: S A
authors.initials: SA
authors.lastname: Cunningham
authors.affiliation: Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota, USA.
authors.forename: M J
authors.initials: MJ
authors.lastname: Espy
authors.affiliation: Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota, USA.
authors.forename: J R
authors.initials: JR
authors.lastname: Uhl
authors.affiliation: Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota, USA.
authors.forename: M F
authors.initials: MF
authors.lastname: Jones
authors.affiliation: Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota, USA.
authors.forename: E A
authors.initials: EA
authors.lastname: Vetter
authors.affiliation: Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota, USA.
authors.forename: J
authors.initials: J
authors.lastname: Mandrekar
authors.affiliation: Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota, USA.
authors.forename: F R
authors.initials: FR
authors.lastname: Cockerill
authors.affiliation: Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota, USA.
authors.forename: B S
authors.initials: BS
authors.lastname: Pritt
authors.affiliation: Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota, USA.
authors.forename: R
authors.initials: R
authors.lastname: Patel
authors.affiliation: Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota, USA wengn@mayo.edu.
authors.forename: N L
authors.initials: NL
authors.lastname: Wengenack
mesh_terms: D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods* / Q000592:standards*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods* / Q000592:standards*; D012015:Reference Standards
references.citation: J Clin Microbiol. 2001 Jun;39(6):2233-6
references.pmid: 11376062
references.citation: J Clin Microbiol. 2010 Aug;48(8):2929-33
references.pmid: 20519461
references.citation: Clin Infect Dis. 2007 Oct 15;45(8):1056-61
references.pmid: 17879925
references.citation: J Clin Microbiol. 2007 Dec;45(12):4081-4
references.pmid: 17942646
references.citation: Diagn Microbiol Infect Dis. 2006 Mar;54(3):169-75
references.pmid: 16423488
references.citation: J Clin Microbiol. 2002 Jan;40(1):96-100
references.pmid: 11773099
references.citation: J Clin Microbiol. 2009 Jul;47(7):2008-12
references.pmid: 19439539
references.citation: Am J Trop Med Hyg. 2009 Sep;81(3):516-8
references.pmid: 19706924
references.citation: J Clin Microbiol. 2007 Jan;45(1):173-8
references.pmid: 17108077
references.citation: J Clin Microbiol. 2000 Feb;38(2):795-9
references.pmid: 10655387
references.citation: J Clin Microbiol. 2012 Mar;50(3):766-71
references.pmid: 22170906
references.citation: J Clin Microbiol. 2000 Sep;38(9):3187-9
references.pmid: 10970354
references.citation: J Clin Microbiol. 1998 Jan;36(1):191-7
references.pmid: 9431945
references.citation: J Clin Microbiol. 2004 Jun;42(6):2636-43
references.pmid: 15184445
references.citation: J Clin Microbiol. 2001 Jul;39(7):2618-26
references.pmid: 11427579
references.citation: Diagn Microbiol Infect Dis. 2008 Dec;62(4):464-6
references.pmid: 18947959
references.citation: Am J Trop Med Hyg. 2005 Nov;73(5):850-4
references.pmid: 16282292
references.citation: J Clin Microbiol. 2008 Jun;46(6):1996-2001
references.pmid: 18434563
references.citation: J Clin Microbiol. 2005 Jul;43(7):3516-8
references.pmid: 16000494
references.citation: Mol Cell Probes. 2005 Feb;19(1):51-9
references.pmid: 15652220
references.citation: J Clin Microbiol. 2002 Nov;40(11):3922-8
references.pmid: 12409353
references.citation: J Clin Microbiol. 2011 Sep;49(9):3204-8
references.pmid: 21752970
references.citation: Arch Otolaryngol Head Neck Surg. 2008 Nov;134(11):1177-81
references.pmid: 19015447