title: Multifunctional superparamagnetic fe3O4@SiO2 core/shell nanoparticles: design and application for cell imaging.
abstract: Highly biocompatible sub-50-nm monodisperse superparamagnetic Fe3O4@SiO2 core/shell nanoparticles with luminescent silica shells were synthesized by a w/o-microemulsion technique. And then these nanoparticles were coated with the covalently bonded biocompatible polymer poly(ethylene glycol) (PEG) and modified with the biological cancer targeting ligand folic acid (FA). After characterized by means of powder X-ray diffraction (XRD), transmission electron microscopy (TEM), dynamic light scattering (DLS), Fourier transformed infrared spectroscopy (FT-IR), Thermogravimetric analysis (TGA), vibrating sample magnetometer (VSM), UV-vis, fluorescence spectroscopy and confocal laser scanning microscopy (CLSM), we confirmed that Fe3O4@SiO2 (FITC)-PEG-FA nanocomposites (SMNPs-FA) could be efficiently taken up by HeLa cancer cells and KB cells which are of over-expression of folate receptors. The multifunctional nanomaterials exhibited superparamagnetic, monodisperse, highly biocompatible, intensively fluorescent and capable of recognizing and binding cells that overexpress folate receptors, which would be useful for targeting cell imaging and provide an excellent platform for further development of an efficient cancer therapy.
authors.forename: Xueling
authors.initials: X
authors.lastname: Zhao
authors.forename: Hongli
authors.initials: H
authors.lastname: Zhao
authors.forename: Huihui
authors.initials: H
authors.lastname: Yuan
authors.forename: Minbo
authors.initials: M
authors.lastname: Lan
mesh_terms: D000818:Animals; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D004705:Endocytosis / Q000187:drug effects; D005492:Folic Acid / Q000494:pharmacology; D006801:Humans; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D051379:Mice; D057054:Molecular Imaging / Q000379:methods*; D041681:NIH 3T3 Cells; D010316:Particle Size; D011208:Powders; D012822:Silicon Dioxide / Q000138:chemical synthesis* / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D013818:Thermogravimetry; D014961:X-Ray Diffraction
chemical_list: D058185:Magnetite Nanoparticles; D011208:Powders; D012822:Silicon Dioxide; D005492:Folic Acid