title: Aversive behavior induced by optogenetic inactivation of ventral tegmental area dopamine neurons is mediated by dopamine D2 receptors in the nucleus accumbens.
abstract: Dopamine (DA) transmission from the ventral tegmental area (VTA) is critical for controlling both rewarding and aversive behaviors. The transient silencing of DA neurons is one of the responses to aversive stimuli, but its consequences and neural mechanisms regarding aversive responses and learning have largely remained elusive. Here, we report that optogenetic inactivation of VTA DA neurons promptly down-regulated DA levels and induced up-regulation of the neural activity in the nucleus accumbens (NAc) as evaluated by Fos expression. This optogenetic suppression of DA neuron firing immediately evoked aversive responses to the previously preferred dark room and led to aversive learning toward the optogenetically conditioned place. Importantly, this place aversion was abolished by knockdown of dopamine D2 receptors but not by that of D1 receptors in the NAc. Silencing of DA neurons in the VTA was thus indispensable for inducing aversive responses and learning through dopamine D2 receptors in the NAc.
authors.affiliation: Department of Systems Biology, Osaka Bioscience Institute, Suita, Osaka 565-0874, Japan.
authors.forename: Teruko
authors.initials: T
authors.lastname: Danjo
authors.forename: Kenji
authors.initials: K
authors.lastname: Yoshimi
authors.forename: Kazuo
authors.initials: K
authors.lastname: Funabiki
authors.forename: Satoshi
authors.initials: S
authors.lastname: Yawata
authors.forename: Shigetada
authors.initials: S
authors.lastname: Nakanishi
mesh_terms: D000818:Animals; D001522:Behavior, Animal; D002755:Choice Behavior; D003213:Conditioning, Psychological; D003624:Darkness; D004298:Dopamine / Q000378:metabolism; D059290:Dopaminergic Neurons / Q000502:physiology*; D015536:Down-Regulation / Q000235:genetics; D005260:Female; D008297:Male; D051379:Mice; D009714:Nucleus Accumbens / Q000378:metabolism*; D062308:Optogenetics / Q000379:methods*; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics / Q000378:metabolism; D017448:Receptors, Dopamine D2 / Q000378:metabolism*; D015398:Signal Transduction / Q000235:genetics; D015854:Up-Regulation / Q000235:genetics; D017557:Ventral Tegmental Area / Q000166:cytology*
chemical_list: D016760:Proto-Oncogene Proteins c-fos; D017448:Receptors, Dopamine D2; D004298:Dopamine
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