title: Effect of lignin content on a GH11 endoxylanase acting on glucuronoarabinoxylan-lignin nanocomposites.
abstract: The effects of lignin content on the activity and action pattern of GH11 endoxylanase from Thermobacillus xylanilyticus were investigated using in vitro reconstituted non-covalent glucuronoarabinoxylan-model lignin (GAX-DHP) nanocomposites. Four types of nanocomposites were prepared, each displaying different lignin contents. Variations in the DHP (model lignin) polymerization process were induced by increasing the coniferyl alcohol concentration. Examination of the morphology of the nanocomposites revealed globular particles enrobed in a matrix. The size of these particles increased in line with the lignin concentration. Physicochemical characterization of the in vitro reconstituted GAX-DHPs strongly suggested that increased particle size is directly related to the solubility and reactivity of coniferyl alcohol, as reflected by changes in the amount of β-O-4 linkages. Evaluation of the impact of the GH11 endoxylanase on the GAX-DHP nanocomposites revealed a negative correlation between the proportion and organization patterns of DHP in the nanocomposites and enzyme activity.
authors.affiliation: INRA, UMR614 Fractionnement des AgroRessources et Environnement, F-51100 Reims, France; Université de Reims Champagne-Ardenne, UMR614 Fractionnement des AgroRessources et Environnement, F-51100 Reims, France.
authors.forename: Imen
authors.initials: I
authors.lastname: Boukari
authors.affiliation: INRA, UMR614 Fractionnement des AgroRessources et Environnement, F-51100 Reims, France; Université de Reims Champagne-Ardenne, UMR614 Fractionnement des AgroRessources et Environnement, F-51100 Reims, France.
authors.forename: Caroline
authors.initials: C
authors.lastname: Rémond
authors.affiliation: Université de Toulouse, INSA, UPS, INP, LISBP, F-31077 Toulouse, France; INRA, UMR792 Ingénierie des Systèmes Biologiques et des Procédés, F-31400 France; CNRS, UMR5504, F-31400 Toulouse, France.
authors.forename: Michael
authors.initials: M
authors.lastname: O'Donohue
authors.affiliation: INRA, UMR614 Fractionnement des AgroRessources et Environnement, F-51100 Reims, France; Université de Reims Champagne-Ardenne, UMR614 Fractionnement des AgroRessources et Environnement, F-51100 Reims, France.
authors.forename: Brigitte
authors.initials: B
authors.lastname: Chabbert
mesh_terms: D043364:Endo-1,4-beta Xylanases / Q000737:chemistry*; D008031:Lignin / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D010636:Phenols / Q000737:chemistry; D058105:Polymerization; D013499:Surface Properties; D014990:Xylans / Q000737:chemistry*
chemical_list: D010636:Phenols; D014990:Xylans; C114470:glucuronoarabinoxylan; D008031:Lignin; C010559:coniferyl alcohol; D043364:Endo-1,4-beta Xylanases