title: Microsatellite markers derived from Japanese scallop (Mizuhopecten yessoensis) expressed sequence tags.
abstract: Japanese scallop (Mizuhopecten yessoensis) is a cold-water shellfish, and a species of economic importance in China. In this study, we developed and evaluated simple sequence repeat (SSR) markers from the expressed sequence tags (ESTs) of M. yessoensis. The characteristics of 12 EST-SSR loci were investigated in 30 individual scallops, and the result revealed that the number of alleles per locus ranged from 2-4, with an observed heterozygosity ranging from 0.0333-0.7692, and an expected heterozygosity ranging from 0.0333-0.6312. Only two loci were found to depart significantly from the Hardy-Weinberg equilibrium (P < 0.05). The result of our study suggested that these markers could be considered as potential markers for studying the population structure of M. yessoensis and its intraspecific variation.
authors.affiliation: Key Laboratory of Marine Fishery Molecular Biology, Liaoning Ocean and Fisheries Science Research Institute, Dalian, China.
authors.forename: Y F
authors.initials: YF
authors.lastname: Li
authors.affiliation: Key Laboratory of Marine Fishery Molecular Biology, Liaoning Ocean and Fisheries Science Research Institute, Dalian, China.
authors.forename: X G
authors.initials: XG
authors.lastname: Gao
authors.affiliation: Key Laboratory of Marine Fishery Molecular Biology, Liaoning Ocean and Fisheries Science Research Institute, Dalian, China.
authors.forename: W D
authors.initials: WD
authors.lastname: Liu
authors.affiliation: College of Fisheries and Life Science, Dalian Ocean University, Dalian, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Tian
authors.affiliation: Key Laboratory of Marine Fishery Molecular Biology, Liaoning Ocean and Fisheries Science Research Institute, Dalian, China.
authors.forename: L
authors.initials: L
authors.lastname: Gao
authors.affiliation: Key Laboratory of Marine Fishery Molecular Biology, Liaoning Ocean and Fisheries Science Research Institute, Dalian, China.
authors.forename: S L
authors.initials: SL
authors.lastname: Li
authors.affiliation: Key Laboratory of Marine Fishery Molecular Biology, Liaoning Ocean and Fisheries Science Research Institute, Dalian, China hechongbo@hotmail.com.
authors.forename: C B
authors.initials: CB
authors.lastname: He
mesh_terms: D000818:Animals; D020224:Expressed Sequence Tags; D005819:Genetic Markers; D018895:Microsatellite Repeats; D049897:Pectinidae / Q000235:genetics*; D011110:Polymorphism, Genetic
chemical_list: D005819:Genetic Markers