title: Codon-based phylogenetics introduces novel flagellar gene markers to oomycete systematics.
abstract: Oomycete systematics has traditionally been reliant on ribosomal RNA and mitochondrial cytochrome oxidase sequences. Here we report the use of two single-copy protein-coding flagellar genes, PF16 and OCM1, in oomycete systematics, showing their utility in phylogenetic reconstruction and species identification. Applying a recently proposed mutation-selection model of codon substitution, the phylogenetic relationships inferred by flagellar genes are largely in agreement with the current views of oomycete evolution, whereas nucleotide- and amino acid-level models produce biologically implausible reconstructions. Interesting parallels exist between the phylogeny inferred from the flagellar genes and zoospore ontology, providing external support for the tree obtained using the codon model. The resolution achieved for species identification is ample using PF16, and quite robust using OCM1, and the described PCR primers are able to amplify both genes for a range of oomycete genera. Altogether, when analyzed with a rich codon substitution model, these flagellar genes provide useful markers for the oomycete molecular toolbox.
authors.affiliation: Agriculture and Agri-Food Canada, 960 Carling Ave., Ottawa, ON K1A 0C6, Canada; Department of Biology, Carleton University, 1125 Colonel By Dr., Ottawa, ON K1S 5B6, Canada. Electronic address: gregg.robideau@inspection.gc.ca.
authors.forename: Gregg P
authors.initials: GP
authors.lastname: Robideau
authors.affiliation: Agriculture and Agri-Food Canada, 960 Carling Ave., Ottawa, ON K1A 0C6, Canada. Electronic address: nicolas.rodrigue@ucalgary.ca.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Rodrigue
authors.affiliation: Agriculture and Agri-Food Canada, 960 Carling Ave., Ottawa, ON K1A 0C6, Canada; Department of Biology, Carleton University, 1125 Colonel By Dr., Ottawa, ON K1S 5B6, Canada. Electronic address: andre.levesque@agr.gc.ca.
authors.forename: C
authors.initials: C
authors.lastname: André Lévesque
mesh_terms: D001499:Bayes Theorem; D002467:Cell Nucleus / Q000235:genetics; D003062:Codon; D005407:Flagella / Q000235:genetics*; D005819:Genetic Markers; D016013:Likelihood Functions; D008957:Models, Genetic; D009868:Oomycetes / Q000145:classification* / Q000235:genetics; D010802:Phylogeny; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D003062:Codon; D005819:Genetic Markers
keywords: Codon substitution model; Flagella; Oomycete; Phylogenetics; Taxonomic marker