title: Spectroscopic investigations of the interaction of the anti‑hypertension drug valsartan with human serum albumin.
abstract: The aim of the present study was to investigate the interaction between valsartan, an anti-hypertension drug, and human serum albumin (HSA) using spectroscopic techniques, including fluorescence, ultraviolet-visible absorption, synchronous fluorescence and circular dichroism (CD). The results demonstrated that valsartan and HSA form a complex and that a static quenching mechanism occurs. In addition, the binding constant and the number of binding sites for valsartan on HSA were analyzed. Hydrophobic interactions and hydrogen bonds were the predominant forces in the association reaction based on thermodynamic parameters. The distance between the donor (HSA) and the acceptor (valsartan) was 1.994 nm as derived from Forster's theory. Alterations in the secondary structure of HSA in the presence of valsartan were assessed using synchronous fluorescence and CD. This study provides an enhanced understanding of the pharmacodynamic effects of valsartan on the physiologically important protein HSA.
authors.affiliation: Department of Bioorganic and Biological Chemistry, Beijing Key Lab, Beijing Normal University, Beijing 100875, P.R. China.
authors.forename: Jian
authors.initials: J
authors.lastname: Jing
authors.affiliation: Department of Bioorganic and Biological Chemistry, Beijing Key Lab, Beijing Normal University, Beijing 100875, P.R. China.
authors.forename: Xin
authors.initials: X
authors.lastname: Qu
authors.affiliation: Department of Bioorganic and Biological Chemistry, Beijing Key Lab, Beijing Normal University, Beijing 100875, P.R. China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Tu
authors.affiliation: Department of Bioorganic and Biological Chemistry, Beijing Key Lab, Beijing Normal University, Beijing 100875, P.R. China.
authors.forename: Chenhoo
authors.initials: C
authors.lastname: Zheng
authors.affiliation: Department of Bioorganic and Biological Chemistry, Beijing Key Lab, Beijing Normal University, Beijing 100875, P.R. China.
authors.forename: Zhicheng
authors.initials: Z
authors.lastname: Zheng
mesh_terms: D000959:Antihypertensive Agents / Q000737:chemistry / Q000378:metabolism*; D002942:Circular Dichroism; D031541:Fluorescence Resonance Energy Transfer; D006801:Humans; D011485:Protein Binding; D012709:Serum Albumin / Q000737:chemistry / Q000378:metabolism*; D000075462:Serum Albumin, Human; D013050:Spectrometry, Fluorescence; D013777:Tetrazoles / Q000737:chemistry / Q000378:metabolism*; D014633:Valine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D000068756:Valsartan
chemical_list: C422455:ALB protein, human; D000959:Antihypertensive Agents; D012709:Serum Albumin; D013777:Tetrazoles; D000068756:Valsartan; D014633:Valine; D000075462:Serum Albumin, Human