title: Isolation and screened neuroprotective active constituents from the roots and rhizomes of Valeriana amurensis.
abstract: In previous study, we have screened the effective fraction against Alzheimer's disease (AD-EF) from the extracts of roots and rhizomes of Valeriana amurensis, based on which neuroprotective active constituents from AD-EF were investigated. Six new compounds 1-6, including four iridoids (xiecaoside A-C and xiecaoline A), one pinane-type monoterpeneglucoside (xiecaoside D), and one phenylpropanoid glycoside (xiecaoside E) were isolated together with 11 known compounds 7-17. The structures of 1-6 were elucidated by their spectroscopic data. The protective effects of compounds 1-17 on PC12 cells with neurotoxicity induced by amyloid-beta 1-42 (Aβ(1-42)) was also investigated, respectively. Consequently, compound 6 and lignans 11-17 were responsible for protecting against Aβ-induced toxicity in PC12 cells.
authors.affiliation: Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, No. 24 HePing Road, XiangFang District, Harbin 150040, China. Electronic address: wangxiaolong831124@163.com.
authors.forename: Changfu
authors.initials: C
authors.lastname: Wang
authors.affiliation: Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, No. 24 HePing Road, XiangFang District, Harbin 150040, China. Electronic address: yunmeng2646@126.com.
authors.forename: Yang
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, No. 24 HePing Road, XiangFang District, Harbin 150040, China. Electronic address: ybywater@163.com.
authors.forename: Bingyou
authors.initials: B
authors.lastname: Yang
authors.affiliation: Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, No. 24 HePing Road, XiangFang District, Harbin 150040, China. Electronic address: wzbmailbox@126.com.
authors.forename: Zhibin
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, No. 24 HePing Road, XiangFang District, Harbin 150040, China. Electronic address: wulihua2009a@sina.com.
authors.forename: Lihua
authors.initials: L
authors.lastname: Wu
authors.affiliation: Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, No. 24 HePing Road, XiangFang District, Harbin 150040, China. Electronic address: sxl2786@163.com.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Su
authors.affiliation: Institute of Pharmacognosy, University of Graz, Universitaetsplatz 4/I, A-8010 Graz, Austria. Electronic address: adelheid.brantner@uni-graz.at.
authors.forename: Adelheid
authors.initials: A
authors.lastname: Brantner
authors.affiliation: Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, No. 24 HePing Road, XiangFang District, Harbin 150040, China. Electronic address: haixuekuang@163.com.
authors.forename: Haixue
authors.initials: H
authors.lastname: Kuang
authors.affiliation: Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, No. 24 HePing Road, XiangFang District, Harbin 150040, China. Electronic address: qhwang668@163.com.
authors.forename: Qiuhong
authors.initials: Q
authors.lastname: Wang
mesh_terms: D000544:Alzheimer Disease / Q000188:drug therapy*; D016229:Amyloid beta-Peptides / Q000633:toxicity; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D039823:Iridoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D017705:Lignans / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D018696:Neuroprotective Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D016716:PC12 Cells; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal; D051381:Rats; D012016:Reference Values; D027343:Rhizome / Q000737:chemistry; D014632:Valerian / Q000737:chemistry*
chemical_list: D016229:Amyloid beta-Peptides; D039823:Iridoids; D017705:Lignans; D018696:Neuroprotective Agents; D010936:Plant Extracts
keywords: Alzheimer’s disease; Iridoids; Lignans; Loganin (PubChem CID: 87691); PC12 cell; Patriscabroside I (PubChem CID: 71453062); Valeriana amurensis