title: Ultrasensitive enzyme-free electrochemical immunoassay for free thyroxine based on three dimensionally ordered macroporous chitosan-Au nanoparticles hybrid film.
abstract: The measurement of free thyroxine concentration in serum is considered to be an essential indicator of thyroid function. Here, a novel enzyme-free sandwich electrochemical immunosensor for the detection of FT4 antigen based on the immobilization of primary antibody (Ab1) on three dimensional ordered macroporous chitosan-Au nanoparticles hybrid (3DOM CS-AuNPs) film electrode, and magnetic multiwall carbon nanotubes (MMWCNTs) were used as label of secondary antibody (Ab2). The 3DOM CS-AuNPs film electrode was constructed by one-step electrodeposition of CS-AuNPs composite onto Au electrode with silica opal template. MMWCNTs were prepared by chemical co-precipitation of Fe(2+) and Fe(3+) salts on carboxylated MWCNTs. Ru(bpy)3(2+) labeled anti-FT4 (Ru(bpy)3(2+)-Ab2) was covalently attached to MMWCNTs through the formation of amide bond between the carboxylic groups of MWCNTs and the amine groups of antibody. Under the optimal conditions, FT4 was detected in a concentration range from 0.71 fg mL(-1) to 1.15 pg mL(-1) with a correlation coefficient of 0.998 and a detection limit of 0.20 fg mL(-1). Moreover, the immunosensor showed excellent selectivity, good stability, satisfactory reproducibility and regeneration. Importantly, the developed method was used to assay clinical serum specimens, achieving a good relation with those obtained from the commercialized electrochemiluminescent method.
authors.affiliation: College of Sciences, Nanjing Tech University, Nanjing 211816, PR China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: College of Sciences, Nanjing Tech University, Nanjing 211816, PR China. Electronic address: chenxj_njut@126.com.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Chen
authors.affiliation: Nanjing Sichuang Biotechnology Co. Ltd., Nanjing 211800, PR China.
authors.forename: Fulai
authors.initials: F
authors.lastname: Tu
authors.affiliation: College of Sciences, Nanjing Tech University, Nanjing 211816, PR China. Electronic address: yaocheng@njtech.edu.cn.
authors.forename: Cheng
authors.initials: C
authors.lastname: Yao
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation; D048271:Chitosan / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000295:instrumentation*; D004867:Equipment Design; D006046:Gold / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation; D057230:Limit of Detection; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D037742:Nanotubes, Carbon / Q000737:chemistry / Q000648:ultrastructure; D015203:Reproducibility of Results; D013974:Thyroxine / Q000032:analysis / Q000097:blood*
chemical_list: D055910:Antibodies, Immobilized; D037742:Nanotubes, Carbon; D006046:Gold; D048271:Chitosan; D013974:Thyroxine
keywords: Chitosan–Au nanoparticles hybrid film; Free thyroxine; Magnetic multiwall carbon nanotubes; Sandwich-type electrochemical immunosensor; Three dimensionally ordered macroporous materials