title: Antifungal susceptibility of Aspergillus fumigatus clinical isolates collected from various areas in Japan.
abstract: Azole resistance among clinical isolates of Aspergillus fumigatus is becoming a serious problem in Europe, but the status in Japan is not yet known in detail. The aim of this study was to determine the present status of azole resistance in A. fumigatus in Japan. We employed 171 clinical isolates of A. fumigatus sensu stricto collected from 1987 to 2008 at the Medical Mycology Research Center, Chiba University, Japan for azole resistance determination. Identification of all isolates were re-examined both from the aspect of morphology and molecular phylogeny. The antifungal susceptibility of these isolates was tested based on the CLSI M38-A2 broth microdilution method. In our collection, only 1 (0.6%) and 2 isolates (1.2%) showed elevated MIC to voriconazole and itraconazole, respectively. Our study disclosed that the frequency of azole resistance in A. fumigatus still remains low in this collection.
authors.affiliation: Medical Mycology Research Center, Chiba University, Japan.
authors.forename: Kazuyo
authors.initials: K
authors.lastname: Kikuchi
authors.affiliation: Medical Mycology Research Center, Chiba University, Japan; Division of Control and Treatment of Infectious Diseases, Chiba University Hospital, Japan. Electronic address: fewata@faculty.chiba-u.jp.
authors.forename: Akira
authors.initials: A
authors.lastname: Watanabe
authors.affiliation: Medical Mycology Research Center, Chiba University, Japan.
authors.forename: Junko
authors.initials: J
authors.lastname: Ito
authors.affiliation: Medical Mycology Research Center, Chiba University, Japan.
authors.forename: Yukio
authors.initials: Y
authors.lastname: Oku
authors.affiliation: Medical Mycology Research Center, Chiba University, Japan.
authors.forename: Tuya
authors.initials: T
authors.lastname: Wuren
authors.affiliation: Medical Mycology Research Center, Chiba University, Japan.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Taguchi
authors.affiliation: Medical Mycology Research Center, Chiba University, Japan.
authors.forename: Kyoko
authors.initials: K
authors.lastname: Yarita
authors.affiliation: Medical Mycology Research Center, Chiba University, Japan.
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Muraosa
authors.affiliation: Medical Mycology Research Center, Chiba University, Japan.
authors.forename: Maki
authors.initials: M
authors.lastname: Yahiro
authors.affiliation: Medical Mycology Research Center, Chiba University, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Yaguchi
authors.affiliation: Medical Mycology Research Center, Chiba University, Japan; Division of Control and Treatment of Infectious Diseases, Chiba University Hospital, Japan.
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Kamei
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D001232:Aspergillus fumigatus / Q000187:drug effects*; D025141:Drug Resistance, Fungal; D006801:Humans; D008826:Microbial Sensitivity Tests
chemical_list: D000935:Antifungal Agents
keywords: Azole resistance; Beta-tubulin gene; Itraconazole; Voriconazole