title: Protection of SK-N-MC cells against β-amyloid peptide-induced degeneration using neuron growth factor-loaded liposomes with surface lactoferrin.
abstract: A liposomal system with surface lactoferrin (Lf) was developed for delivering neuron growth factor (NGF) across the blood-brain barrier (BBB) and improving the viability of neuron-like SK-N-MC cells with deposited β-amyloid peptide (Aβ). The Lf-grafted liposomes carrying NGF (Lf/NGF-liposomes) were applied to a monolayer of human brain-microvascular endothelial cells (HBMECs) regulated by human astrocytes (HAs) and to fibrillar Aβ1-42-insulted SK-N-MC cells. An increase in cholesterol mole percentage enhanced the particle size, absolute value of zeta potential, and physical stability, however, reduced the entrapment efficiency and release rate of NGF. In addition, an increase in Lf concentration increased the particle size, surface nitrogen percentage, NGF permeability across the BBB, and viability of HBMECs, HAs, and SK-N-MC cells, however, decreased the absolute value of zeta potential, surface phosphorus percentage, and loading efficiency of Lf. After treating with Lf/NGF-liposomes, a higher Aβ concentration yielded a lower survival of SK-N-MC cells. The current Lf/NGF-liposomes are efficacious drug carriers to target the BBB and inhibit the Aβ-induced neurotoxicity as potential pharmacotherapy for Alzheimer's disease.
authors.affiliation: Department of Chemical Engineering, National Chung Cheng University, Chia-Yi 62102, Taiwan, ROC. Electronic address: chmyck@ccu.edu.tw.
authors.forename: Yung-Chih
authors.initials: YC
authors.lastname: Kuo
authors.affiliation: Department of Chemical Engineering, National Chung Cheng University, Chia-Yi 62102, Taiwan, ROC.
authors.forename: Cheng-Ting
authors.initials: CT
authors.lastname: Wang
mesh_terms: D000544:Alzheimer Disease / Q000378:metabolism; D016229:Amyloid beta-Peptides / Q000378:metabolism*; D001812:Blood-Brain Barrier / Q000378:metabolism; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D007781:Lactoferrin / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D009414:Nerve Growth Factors / Q000008:administration & dosage* / Q000494:pharmacology*
chemical_list: D016229:Amyloid beta-Peptides; D008081:Liposomes; D009414:Nerve Growth Factors; D007781:Lactoferrin
keywords: Alzheimer's disease; Blood–brain barrier; Lactoferrin; Liposome; Neuron growth factor; SK-N-MC cell