title: Novel thermostable endo-xylanase cloned and expressed from bacterium Geobacillus sp. WSUCF1.
abstract: A gene encoding a GH10 endo-xylanase from Geobacillus sp. WSUCF1 was cloned and expressed in Escherichia coli. Recombinant endo-xylanase (37kDa) exhibited high specific activity of 461.0U/mg of protein. Endo-xylanase was optimally active on birchwood xylan at 70°C and pH 6.5. The endo-xylanase was found to be highly thermostable at 50 and 60°C, retaining 82% and 50% of its original activity, respectively, after 60h. High xylan conversions (92%) were obtained with oat-spelt xylan hydrolysis. Higher glucan and xylan conversions were obtained on AFEX-treated corn stover with an enzyme cocktail containing WSUCF1 endo-xylanase (71% and 47%) as compared to enzyme cocktail containing commercial fungal endo-xylanase (64% and 41%). High specific activity, active at high pH's, wide substrate specificity, and higher hydrolytic activity on recalcitrant lignocellulose, make this endo-xylanase a suitable candidate for biofuel and bioprocess industries.
authors.affiliation: Department of Chemical and Biological Engineering, South Dakota School of Mines and Technology, Rapid City, SD 57701, USA.
authors.forename: Aditya
authors.initials: A
authors.lastname: Bhalla
authors.affiliation: Renewable Product Technology Research Unit, Agricultural Research Service, National Center for Agricultural Utilization Research, U.S. Department of Agriculture, Peoria, IL 61604, USA.
authors.forename: Kenneth M
authors.initials: KM
authors.lastname: Bischoff
authors.affiliation: Department of Chemical Engineering and Materials Science, DOE Great Lakes Bioenergy Research Center, Michigan State University, Lansing, MI 48823, USA.
authors.forename: Nirmal
authors.initials: N
authors.lastname: Uppugundla
authors.affiliation: Department of Chemical Engineering and Materials Science, DOE Great Lakes Bioenergy Research Center, Michigan State University, Lansing, MI 48823, USA.
authors.forename: Venkatesh
authors.initials: V
authors.lastname: Balan
authors.affiliation: Department of Chemical and Biological Engineering, South Dakota School of Mines and Technology, Rapid City, SD 57701, USA. Electronic address: Rajesh.Sani@sdsmt.edu.
authors.forename: Rajesh K
authors.initials: RK
authors.lastname: Sani
mesh_terms: D018554:Avena / Q000378:metabolism; D001483:Base Sequence; D029662:Betula / Q000378:metabolism; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D043364:Endo-1,4-beta Xylanases / Q000378:metabolism*; D004795:Enzyme Stability; D005658:Fungi / Q000201:enzymology; D056506:Geobacillus / Q000201:enzymology*; D006868:Hydrolysis; D008031:Lignin / Q000378:metabolism; D010802:Phylogeny; D011994:Recombinant Proteins / Q000378:metabolism; D013696:Temperature; D014990:Xylans / Q000378:metabolism
chemical_list: D011994:Recombinant Proteins; D014990:Xylans; C036909:lignocellulose; D008031:Lignin; D043364:Endo-1,4-beta Xylanases
keywords: AFEX-treated corn stover; Biofuels; Endo-xylanase; Thermostable