title: Conformational changes in human Hsp70 induced by high hydrostatic pressure produce oligomers with ATPase activity but without chaperone activity.
abstract: We investigated the folding of the 70 kDa human cytosolic inducible protein (Hsp70) in vitro using high hydrostatic pressure as a denaturing agent. We followed the structural changes in Hsp70 induced by high hydrostatic pressure using tryptophan fluorescence, molecular dynamics, circular dichroism, high-performance liquid chromatography gel filtration, dynamic light scattering, ATPase activity, and chaperone activity. Although monomeric, Hsp70 is very sensitive to hydrostatic pressure; after pressure had been removed, the protein did not return to its native sate but instead formed oligomeric species that lost chaperone activity but retained ATPase activity.
authors.affiliation: Instituto de Bioquímica Médica Leopoldo de Meis, Programa de Biologia Estrutural, Universidade Federal do Rio de Janeiro , Rio de Janeiro 21941-590, Brazil.
authors.forename: Thaís L S
authors.initials: TL
authors.lastname: Araujo
authors.forename: Julio Cesar
authors.initials: JC
authors.lastname: Borges
authors.forename: Carlos H
authors.initials: CH
authors.lastname: Ramos
authors.forename: José Roberto
authors.initials: JR
authors.lastname: Meyer-Fernandes
authors.forename: Reinaldo S
authors.initials: RS
authors.lastname: Oliveira Júnior
authors.forename: Pedro G
authors.initials: PG
authors.lastname: Pascutti
authors.forename: Debora
authors.initials: D
authors.lastname: Foguel
authors.forename: Fernando L
authors.initials: FL
authors.lastname: Palhano
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D002942:Circular Dichroism; D018840:HSP70 Heat-Shock Proteins / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D006874:Hydrostatic Pressure; D018832:Molecular Chaperones / Q000378:metabolism*; D011487:Protein Conformation; D011489:Protein Denaturation; D017510:Protein Folding
chemical_list: D018840:HSP70 Heat-Shock Proteins; D018832:Molecular Chaperones; D000251:Adenosine Triphosphatases