title: Triazole-based Zn²⁺-specific molecular marker for fluorescence bioimaging.
abstract: Fluorescence bioimaging potential, both in vitro and in vivo, of a yellow emissive triazole-based molecular marker has been investigated and demonstrated. Three different kinds of cells, viz Bacillus thuringiensis, Candida albicans, and Techoma stans pollen grains were used to investigate the intracellular zinc imaging potential of 1 (in vitro studies). Fluorescence imaging of translocation of zinc through the stem of small herb, Peperomia pellucida, having transparent stem proved in vivo bioimaging capability of 1. This approach will enable in screening cell permeability and biostability of a newly developed probe. Similarly, the current method for detection and localization of zinc in Gram seed sprouts could be an easy and potential alternative of the existing analytical methods to investigate the efficiency of various strategies applied for increasing zinc-content in cereal crops. The probe-zinc ensemble has efficiently been applied for detecting phosphate-based biomolecules.
authors.affiliation: School of Basic Sciences, Indian Institute of Technology Mandi, Mandi, 175001 H.P, India.
authors.forename: Sougata
authors.initials: S
authors.lastname: Sinha
authors.affiliation: Department of Microbiology, Burdwan University, Burdwan, W.B., India.
authors.forename: Trinetra
authors.initials: T
authors.lastname: Mukherjee
authors.affiliation: Schulich Faculty of Chemistry and the Lise Meitner Minerva Center for Computational Quantum Chemistry, Technion-Israel Institute of Technology, Haifa, Israel.
authors.forename: Jomon
authors.initials: J
authors.lastname: Mathew
authors.affiliation: Department of Microbiology, Burdwan University, Burdwan, W.B., India. Electronic address: skmmicro@gmail.com.
authors.forename: Subhra K
authors.initials: SK
authors.lastname: Mukhopadhyay
authors.affiliation: School of Basic Sciences, Indian Institute of Technology Mandi, Mandi, 175001 H.P, India. Electronic address: subrata@iitmandi.ac.in.
authors.forename: Subrata
authors.initials: S
authors.lastname: Ghosh
mesh_terms: D001413:Bacillus thuringiensis / Q000166:cytology / Q000378:metabolism; D002176:Candida albicans / Q000166:cytology / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry*; D008856:Microscopy, Fluorescence; D015335:Molecular Probes / Q000737:chemistry / Q000378:metabolism*; D026321:Piperaceae / Q000737:chemistry / Q000378:metabolism; D018547:Plant Stems / Q000737:chemistry / Q000378:metabolism; D011789:Quantum Theory; D013056:Spectrophotometry, Ultraviolet; D014230:Triazoles / Q000737:chemistry*; D015032:Zinc / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D015335:Molecular Probes; D014230:Triazoles; D015032:Zinc
keywords: Fluorescence bioimaging; Phosphate detection; Yellow emissive marker; Zinc translocation