title: Region-specific DNA alterations in focally induced seizures.
abstract: We induced brief secondarily generalized seizures of limbic origin in Sprague-Dawley rats by bicuculline microinfusion into the anterior piriform cortex. After 1 h or 5 days we performed comet assay, a sensitive marker for DNA damage, within entorhinal cortex, hippocampus (limbic areas recruited by seizure spreading) and striatum (which is not recruited). DNA damage occurred selectively in the ipsilateral entorhinal cortex and hippocampus at 1 h, but not at 5 days. These data shed new light on molecular genetics as a marker during limbic seizures, the most common in epileptic patients.
authors.affiliation: Department of Clinical and Experimental Medicine, University of Pisa, Pisa, Italy.
authors.forename: Emanuela
authors.initials: E
authors.lastname: Cantafora
authors.forename: Filippo Sean
authors.initials: FS
authors.lastname: Giorgi
authors.forename: Giada
authors.initials: G
authors.lastname: Frenzilli
authors.forename: Vittoria
authors.initials: V
authors.lastname: Scarcelli
authors.forename: Carla L
authors.initials: CL
authors.lastname: Busceti
authors.forename: Marco
authors.initials: M
authors.lastname: Nigro
authors.forename: Margherita
authors.initials: M
authors.lastname: Bernardeschi
authors.forename: Francesco
authors.initials: F
authors.lastname: Fornai
mesh_terms: D000818:Animals; D001640:Bicuculline / Q000031:analogs & derivatives / Q000633:toxicity; D001921:Brain / Q000378:metabolism*; D020552:Comet Assay; D003292:Convulsants / Q000633:toxicity; D004249:DNA Damage / Q000187:drug effects; D004195:Disease Models, Animal; D004569:Electroencephalography; D008297:Male; D015999:Multivariate Analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D012640:Seizures / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology*
chemical_list: D003292:Convulsants; C017069:bicuculline methiodide; D001640:Bicuculline
references.citation: Neurobiol Dis. 2008 Jun;30(3):388-399
references.pmid: 18395460
references.citation: Epilepsy Res. 2002 Jun;50(1-2):79-92
references.pmid: 12151120
references.citation: Synapse. 1989;3(2):154-71
references.pmid: 2648633
references.citation: Mutagenesis. 2008 May;23(3):171-82
references.pmid: 18385511
references.citation: Ann N Y Acad Sci. 2006 Aug;1074:478-81
references.pmid: 17105946
references.citation: J Neurosci. 1992 Aug;12(8):3004-9
references.pmid: 1494943
references.citation: Environ Health Perspect. 2003 Apr;111(4):467-71
references.pmid: 12676600
references.citation: Exp Cell Res. 2000 Apr 10;256(1):328-37
references.pmid: 10739681
references.citation: Eur J Neurosci. 2003 Jun;17(12):2593-601
references.pmid: 12823466
references.citation: Neurosci Lett. 2012 May 2;515(2):137-40
references.pmid: 22450051
references.citation: Neuroscience. 1985 Feb;14(2):375-403
references.pmid: 2859548
references.citation: Behav Pharmacol. 2007 Sep;18(5-6):471-81
references.pmid: 17762515
references.citation: Nature. 1985 Oct 17-23;317(6038):623-5
references.pmid: 4058572
references.citation: Environ Health Perspect. 2004 Dec;112(17):1671-2
references.pmid: 15579411
references.citation: J Neurosci. 2011 May 25;31(21):7648-56
references.pmid: 21613478
references.citation: Epilepsia. 2006;47 Suppl 5:21-5
references.pmid: 17239101
references.citation: Environ Mol Mutagen. 2000;35(3):206-21
references.pmid: 10737956