title: Intranasal delivery of nanoparticles encapsulating BPI3V proteins induces an early humoral immune response in mice.
abstract: Vaccine adjuvants are typically designed to stimulate both systemic and mucosal immune responses. Polymeric nanoparticles have been used as adjuvants in the development of vaccines against a number of viral pathogens and tested in laboratory animals. The objective of the study was to assess if synthetic bovine parainfluenza virus type-3 (BPI3V) peptide motifs and solubilised BPI3V proteins encapsulated in poly (dl-lactic-co-glycolide) (PLGA) nanoparticles (NPs) induce specific humoral immune responses in a mouse model following intranasal administration. BPI3V-specific and peptide specific IgG ELISAs were used to measure serum IgG levels to BPI3V. Intranasal delivery of PLGA nanoparticles encapsulating BPI3V proteins elicited an early, gradually increasing BPI3V-specific IgG response that persisted over the subsequent 6 weeks, suggesting slow, persistent release of antigen. PLGA-BPI3V particles administered intranasally induced a stronger IgG antibody response at an earlier time point compared with solubilised BPI3V antigen alone. Such an approach could be deployed in the development of new generation vaccines.
authors.affiliation: Agri Food Biosciences Institute, Veterinary Sciences Division, Stoney Road, Stormont, Belfast BT4 3SD, UK.
authors.forename: Fawad
authors.initials: F
authors.lastname: Mansoor
authors.affiliation: Animal and Bioscience Research Department, Animal & Grassland Research and Innovation Centre, Teagasc, Grange, Dunsany, Co. Meath, Ireland.
authors.forename: Bernadette
authors.initials: B
authors.lastname: Earley
authors.affiliation: Veterinary Sciences Centre, School of Agriculture, Food Science and Veterinary Medicine, University College Dublin, Belfield, Ireland.
authors.forename: Joseph P
authors.initials: JP
authors.lastname: Cassidy
authors.affiliation: Veterinary Sciences Centre, School of Agriculture, Food Science and Veterinary Medicine, University College Dublin, Belfield, Ireland.
authors.forename: Bryan
authors.initials: B
authors.lastname: Markey
authors.affiliation: Agri Food Biosciences Institute, Veterinary Sciences Division, Stoney Road, Stormont, Belfast BT4 3SD, UK.
authors.forename: Colin
authors.initials: C
authors.lastname: Foster
authors.affiliation: Agri Food Biosciences Institute, Veterinary Sciences Division, Stoney Road, Stormont, Belfast BT4 3SD, UK.
authors.forename: Simon
authors.initials: S
authors.lastname: Doherty
authors.affiliation: Agri Food Biosciences Institute, Veterinary Sciences Division, Stoney Road, Stormont, Belfast BT4 3SD, UK. Electronic address: michael.welsh@afbini.gov.uk.
authors.forename: Michael D
authors.initials: MD
authors.lastname: Welsh
mesh_terms: D000281:Administration, Intranasal / Q000662:veterinary; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000517:prevention & control / Q000821:virology*; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D056724:Immunity, Humoral / Q000276:immunology; D019344:Lactic Acid / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D053758:Nanoparticles / Q000008:administration & dosage*; D029101:Parainfluenza Virus 3, Bovine / Q000276:immunology*; D011100:Polyglycolic Acid / Q000494:pharmacology*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011897:Random Allocation; D010253:Respirovirus Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary* / Q000821:virology; D014765:Viral Vaccines / Q000276:immunology* / Q000592:standards
chemical_list: D000914:Antibodies, Viral; D014765:Viral Vaccines; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid
keywords: Immunity; Nanoparticles; Parainfluenza virus type-3 (BPI3V); Respiratory disease; Vaccine