title: Anti-CD133 antibody immobilized on the surface of stents enhances endothelialization.
abstract: Drug eluting stents successfully reduce restenosis at the cost of delayed reendothelialization. In recent years, a novel concept to enhance reendothelialization using anti-CD34 antibody coated stents which capture circulating progenitor cells (EPCs) has been developed with conflicting clinical results. CD133 is a glycoprotein expressed on circulating hematopoietic and putative endothelial-regenerating cells and may be superior to CD34 for EPCs capture stents. In the present study, anti-CD133 antibody has been successfully immobilized to the biodegradable polymeric coating material by covalent conjugation. We explore whether anti-CD133 antibody coated stents (CD133 stents) might accelerate reendothelialization in comparison with bare metal stents (BMS) through the superior ability to capture EPCs. The in vitro cell culture results indicate that anti-CD133 antibody functionalized polymer film significantly promotes CD133 positive cells attachment and growth compared with the unfunctionalized polymer film. In the semi-in vivo arteriovenous shunt model CD133 stents demonstrate much quicker specific capturing of EPCs from the blood stream than BMS within 6 hours. In a porcine coronary artery injury model CD133 stents show more effective reendothelialization in short term compared with BMS, while no significant difference in endothelial function recovery was observed between these two groups within 6-month followup.
authors.affiliation: Department of Cardiology, The Affiliated Hospital of Medical College, Qingdao University, Qingdao Shandong 266003, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Cardiology, The Affiliated Hospital of Medical College, Qingdao University, Qingdao Shandong 266003, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Li
authors.affiliation: Key Laboratory for Ultrafine Materials of Ministry of Education, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Feirong
authors.identifier: 0000-0002-1530-9081
authors.initials: F
authors.lastname: Gong
authors.affiliation: Department of Cardiology, The Affiliated Hospital of Medical College, Qingdao University, Qingdao Shandong 266003, China.
authors.forename: Shaoyan
authors.initials: S
authors.lastname: Jiang
authors.affiliation: Department of Cardiology, The Affiliated Hospital of Medical College, Qingdao University, Qingdao Shandong 266003, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Yu
authors.affiliation: Department of Cardiology, The Affiliated Hospital of Medical College, Qingdao University, Qingdao Shandong 266003, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: An
mesh_terms: D000071916:AC133 Antigen; D000818:Animals; D055910:Antibodies, Immobilized / Q000737:chemistry*; D015703:Antigens, CD / Q000276:immunology*; D018952:Antigens, CD34 / Q000378:metabolism; D002469:Cell Separation; D002478:Cells, Cultured; D004727:Endothelium / Q000473:pathology*; D005434:Flow Cytometry; D006023:Glycoproteins / Q000276:immunology*; D006412:Hematopoietic Stem Cells / Q000166:cytology; D008670:Metals / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D010455:Peptides / Q000276:immunology*; D011108:Polymers / Q000737:chemistry; D013234:Stem Cells / Q000166:cytology; D015607:Stents; D013499:Surface Properties; D013552:Swine
chemical_list: D000071916:AC133 Antigen; D055910:Antibodies, Immobilized; D015703:Antigens, CD; D018952:Antigens, CD34; D006023:Glycoproteins; D008670:Metals; D010455:Peptides; D011108:Polymers
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