title: The role of survivin in podocyte injury induced by puromycin aminonucleoside.
abstract: OBJECTIVE
  Survivin is a member of the inhibitor of apoptosis protein family, which uniquely promotes mitosis and regulates apoptosis in cancer cells. Recent studies have demonstrated that survivin also expresses in several normal adult cells. In the present study, we aimed to investigate the function of survivin in the terminally differentiated epithelial cells, podocytes.
  
  
  METHODS
  Survivin expression and location were detected by Quantitative Real-Time PCR, western blot and fluorescence confocal microscopy methods in normal and injured mouse podocytes. Cyto-protection function of survivin was also studied in cultured podocyte injured by puromycin aminonucleoside (PAN), transfected with survivin siRNA to down-regulate survivin expression, or with survivin plasmid to transiently over-express survivin.
  
  
  RESULTS
  In podocytes, PAN stimulated expressions of survivin and the apoptosis related molecule caspase 3. Knockdown of survivin expression by siRNA increased the activation of caspase 3, induced podocyte apoptosis and remarkable rearrangement of actin cytoskeleton. Moreover, over-expression of survivin inhibited PAN-induced podocyte apoptosis and cytoskeleton rearrangement.
  
  
  CONCLUSIONS
  Our data provides the evidence that survivin plays an important role in protecting podocytes from apoptosis induced by PAN. The mechanism of survivin related anti-apoptosis may, at least partially, be through the activation of caspase 3.
authors.affiliation: Department of Pediatrics, Peking University First Hospital, No.1 Xi An Men Da Jie, Beijing 100034, China. clairesnow@126.com.
authors.forename: Xuejuan
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Pediatrics, Peking University First Hospital, No.1 Xi An Men Da Jie, Beijing 100034, China. wserien@163.com.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Pediatrics, Peking University First Hospital, No.1 Xi An Men Da Jie, Beijing 100034, China. xiaoyanli_520@163.com.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Pediatrics, Peking University First Hospital, No.1 Xi An Men Da Jie, Beijing 100034, China. youngbear@126.com.
authors.forename: Fangrui
authors.initials: F
authors.lastname: Ding
authors.affiliation: Department of Pediatrics, Peking University First Hospital, No.1 Xi An Men Da Jie, Beijing 100034, China. djnc_5855@126.com.
authors.forename: Jie
authors.initials: J
authors.lastname: Ding
mesh_terms: D008841:Actin Cytoskeleton / Q000187:drug effects; D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000378:metabolism; D002478:Cells, Cultured; D051034:Inhibitor of Apoptosis Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D018613:Microscopy, Confocal; D050199:Podocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D011692:Puromycin Aminonucleoside / Q000494:pharmacology*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D012097:Repressor Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D000077022:Survivin
chemical_list: D000903:Antibiotics, Antineoplastic; C117749:Birc5 protein, mouse; D051034:Inhibitor of Apoptosis Proteins; D034741:RNA, Small Interfering; D012097:Repressor Proteins; D000077022:Survivin; D011692:Puromycin Aminonucleoside; D053148:Caspase 3
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