title: Efficient generation of monoclonal antibodies against major structural proteins of rabies virus with suckling mouse brain antigen.
abstract: The rabies virus is a neurotropic virus that causes fatal disease in humans and animals. However, not all commercial antibodies against rabies virus (RABV) structural proteins are generally available, and production of high-quality monoclonal antibodies (MAbs) requires high purification of virus particles and special facilities and is time-consuming. By using RABV-infected suckling mouse brain as antigens in this study, 11 hybridoma cells secreting MAbs against RABV were obtained, which showed strong reactivity with RABV-infected Vero cells in immunofluorescence assay. Among the 11 MAbs, three MAbs (1B11, 1C8, and 8H12) showed a neutralizing effect to RABV, while MAb 4B7 recognized the recombinant nucleoprotein (N) of RABV expressed in Vero cells; seven MAbs (1H3, 3H7, 4E7, 4G3, 5A10, 6C9, and 7B3) reacted specifically with phosphoprotein (P) of RABV. The MAbs developed in this study will be useful in establishing a diagnostic test and study on the interactions between RABV and its host.
authors.affiliation: 1 Key Laboratory of Animal Virology of Ministry of Agriculture, Zhejiang University , Hangzhou, P.R. China .
authors.forename: Jinyang
authors.initials: J
authors.lastname: Zhang
authors.forename: Xizhen
authors.initials: X
authors.lastname: Ruan
authors.forename: Jie
authors.initials: J
authors.lastname: Zan
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Zheng
authors.forename: Yan
authors.initials: Y
authors.lastname: Yan
authors.forename: Min
authors.initials: M
authors.lastname: Liao
authors.forename: Jiyong
authors.initials: J
authors.lastname: Zhou
mesh_terms: D000818:Animals; D000833:Animals, Suckling; D058846:Antibodies, Monoclonal, Murine-Derived / Q000096:biosynthesis / Q000737:chemistry* / Q000494:pharmacology; D057134:Antibodies, Neutralizing / Q000096:biosynthesis / Q000737:chemistry* / Q000494:pharmacology; D000956:Antigens, Viral / Q000276:immunology; D001921:Brain / Q000821:virology; D002522:Chlorocebus aethiops; D005260:Female; D006825:Hybridomas; D051379:Mice; D008807:Mice, Inbred BALB C; D011820:Rabies virus / Q000276:immunology*; D014709:Vero Cells; D015678:Viral Structural Proteins / Q000276:immunology*; D014779:Virus Replication / Q000187:drug effects
chemical_list: D058846:Antibodies, Monoclonal, Murine-Derived; D057134:Antibodies, Neutralizing; D000956:Antigens, Viral; D015678:Viral Structural Proteins