title: Different accumulation profiles of multiple components between pericarp and seed of Alpinia oxyphylla capsular fruit as determined by UFLC-MS/MS.
abstract: Plant secondary metabolites are known to not only play a key role in the adaptation of plants to their environment, but also represent an important source of active pharmaceuticals. Alpinia oxyphylla capsular fruits, made up of seeds and pericarps, are commonly used in traditional East Asian medicines. In clinical utilization of these capsular fruits, inconsistent processing approaches (i.e., hulling pericarps or not) are employed, with the potential of leading to differential pharmacological effects. Therefore, an important question arises whether the content levels of pharmacologically active chemicals between the seeds and pericarps of A. oxyphylla are comparable. Nine secondary metabolites present in A. oxyphylla capsular fruits, including flavonoids (e.g., tectochrysin, izalpinin, chrysin, apigenin-4',7-dimethylether and kaempferide), diarylheptanoids (e.g., yakuchinone A and B and oxyphyllacinol) and sesquiterpenes (e.g., nootkatone), were regarded as representative constituents with putative pharmacological activities. This work aimed to investigate the abundance of the nine constituents in the seeds and pericarps of A. oxyphylla. Thirteen batches of A. oxyphylla capsular fruits were gathered from different production regions. Accordingly, an ultra-fast high performance liquid chromatography/quadrupole tandem mass spectrometry (UFLC-MS/MS) method was developed and validated. We found that: (1) the nine secondary metabolites were differentially concentrated in seeds and fruit capsules; (2) nootkatone is predominantly distributed in the seeds; in contrast, the flavonoids and diarylheptanoids are mainly deposited in the capsules; and (3) the content levels of the nine secondary metabolites occurring in the capsules varied greatly among different production regions, although the nootkatone levels in the seeds were comparable among production regions. These results are helpful to evaluating and elucidating pharmacological activities of A. oxyphylla capsular fruits. Additionally, it may be of interest to elucidate the mechanisms involved in the distinct accumulation profiles of these secondary metabolites between seeds and pericarps.
authors.affiliation: School of Pharmacy, Hainan Medical University, Haikou 571101, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Chen
authors.affiliation: School of Pharmacy, Hainan Medical University, Haikou 571101, China.
authors.forename: Hai-Long
authors.initials: HL
authors.lastname: Li
authors.affiliation: School of Pharmacy, Hainan Medical University, Haikou 571101, China.
authors.forename: Yin-Feng
authors.initials: YF
authors.lastname: Tan
authors.affiliation: School of Pharmacy, Hainan Medical University, Haikou 571101, China.
authors.forename: Wei-Wei
authors.initials: WW
authors.lastname: Guan
authors.affiliation: School of Pharmacy, Hainan Medical University, Haikou 571101, China. hy_jqzhang@163.com.
authors.forename: Jun-Qing
authors.initials: JQ
authors.lastname: Zhang
authors.affiliation: School of Pharmacy, Hainan Medical University, Haikou 571101, China. lyhssl@126.com.
authors.forename: Yong-Hui
authors.initials: YH
authors.lastname: Li
authors.affiliation: The Hamner Institutes for Health Sciences, Research Triangle Park, NC 27709, USA.
authors.forename: Yuan-Sheng
authors.initials: YS
authors.lastname: Zhao
authors.affiliation: School of Pharmacy, Hainan Medical University, Haikou 571101, China.
authors.forename: Zhen-Miao
authors.initials: ZM
authors.lastname: Qin
mesh_terms: D032429:Alpinia / Q000737:chemistry*; D002681:China; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002980:Climate; D005419:Flavonoids / Q000145:classification* / Q000302:isolation & purification; D005843:Geography; D009928:Organ Specificity; D010936:Plant Extracts / Q000032:analysis*; D012639:Seeds / Q000737:chemistry*; D012717:Sesquiterpenes / Q000145:classification* / Q000302:isolation & purification; D053719:Tandem Mass Spectrometry
chemical_list: C442779:Alpinia oxyphylla fruit extract; D005419:Flavonoids; D010936:Plant Extracts; D012717:Sesquiterpenes
references.citation: J Med Entomol. 2005 May;42(3):352-8
references.pmid: 15962787
references.citation: Toxicol Lett. 2003 Sep 30;144(2):205-12
references.pmid: 12927364
references.citation: J Environ Pathol Toxicol Oncol. 2002;21(2):131-9
references.pmid: 12086399
references.citation: J Agric Food Chem. 2000 Aug;48(8):3639-41
references.pmid: 10956162
references.citation: Am J Chin Med. 2001;29(2):293-302
references.pmid: 11527071
references.citation: Plant Sci. 2012 Nov;196:67-76
references.pmid: 23017900
references.citation: Cell Mol Neurobiol. 2012 Jan;32(1):27-40
references.pmid: 21744117
references.citation: Chem Pharm Bull (Tokyo). 1990 Nov;38(11):3053-4
references.pmid: 2085887
references.citation: J Econ Entomol. 2001 Dec;94(6):1532-7
references.pmid: 11777060
references.citation: Drug Test Anal. 2014 Mar;6(3):239-45
references.pmid: 23408659
references.citation: Chem Cent J. 2013 Jul 30;7(1):131
references.pmid: 23899222
references.citation: Pest Manag Sci. 2003 Jan;59(1):58-68
references.pmid: 12558100
references.citation: Chem Cent J. 2014 Jan 14;8(1):2
references.pmid: 24422995
references.citation: Arch Pharm Res. 2012 Dec;35(12):2143-6
references.pmid: 23263808
references.citation: J Med Entomol. 2006 Sep;43(5):957-61
references.pmid: 17017233
references.citation: Oncol Res. 2002;13(1):37-45
references.pmid: 12201673
references.citation: FEBS Lett. 2006 Feb 13;580(4):1183-91
references.pmid: 16364309
references.citation: Adv Nutr. 2011 Jan;2(1):32-50
references.pmid: 22211188
references.citation: BMC Complement Altern Med. 2013 Sep 26;13:237
references.pmid: 24070160
references.citation: J Chem Ecol. 2001 Mar;27(3):523-31
references.pmid: 11441443
references.citation: Chem Cent J. 2013 Aug 02;7:134
references.pmid: 23915886
references.citation: Fitoterapia. 2013 Sep;89:149-56
references.pmid: 23583435
references.citation: Planta Med. 2010 Dec;76(17):2004-11
references.pmid: 21077026