title: Chlamydia psittaci inclusion membrane protein IncB associates with host protein Snapin.
abstract: Chlamydia (C.) psittaci, the causative agent of psittacosis in birds and humans, is the most important zoonotic pathogen of the family Chlamydiaceae. During a unique developmental cycle of this obligate intracellular pathogen, the infectious elementary body gains access to the susceptible host cell, where it transforms into the replicative reticulate body. C. psittaci uses dynein motor proteins for optimal early development. Chlamydial proteins that mediate this process are unknown. Two-hybrid screening with the C. psittaci inclusion protein IncB as bait against a HeLa Yeast Two-hybrid (YTH) library revealed that the host protein Snapin interacts with IncB. Snapin is a cytoplasmic protein that plays a multivalent role in intracellular trafficking. Confocal fluorescence microscopy using an IncB-specific antibody demonstrated that IncB, Snapin, and dynein were co-localized near the inclusion of C. psittaci-infected HEp-2 cells. This co-localization was lost when Snapin was depleted by RNAi. The interaction of Snapin with both IncB and dynein has been shown in vitro and in vivo. We propose that Snapin connects chlamydial inclusions with the microtubule network by interacting with both IncB and dynein.
authors.affiliation: Department of Cell and Molecular Biology, Leibniz Institute for Natural Product Research and Infection Biology (Hans Knoell Institute), Beutenbergstrasse 11a, D-07745 Jena, Germany.
authors.forename: Selina
authors.initials: S
authors.lastname: Böcker
authors.affiliation: Department of Cell and Molecular Biology, Leibniz Institute for Natural Product Research and Infection Biology (Hans Knoell Institute), Beutenbergstrasse 11a, D-07745 Jena, Germany.
authors.forename: Adeline
authors.initials: A
authors.lastname: Heurich
authors.affiliation: Department of Cell and Molecular Biology, Leibniz Institute for Natural Product Research and Infection Biology (Hans Knoell Institute), Beutenbergstrasse 11a, D-07745 Jena, Germany.
authors.forename: Claudia
authors.initials: C
authors.lastname: Franke
authors.affiliation: Imaging Facility, Leibniz Institute for Age Research (Fritz Lipmann Institute), Beutenbergstrasse 11, D-07745 Jena, Germany.
authors.forename: Shamci
authors.initials: S
authors.lastname: Monajembashi
authors.affiliation: Friedrich-Loeffler-Institut (Federal Research Institute for Animal Health), Institute of Molecular Pathogenesis, Naumburger Str. 96a, D-07743 Jena, Germany.
authors.forename: Konrad
authors.initials: K
authors.lastname: Sachse
authors.affiliation: Department of Cell and Molecular Biology, Leibniz Institute for Natural Product Research and Infection Biology (Hans Knoell Institute), Beutenbergstrasse 11a, D-07745 Jena, Germany; Friedrich-Schiller-University, D-07745 Jena, Germany.
authors.forename: Hans Peter
authors.initials: HP
authors.lastname: Saluz
authors.affiliation: Department of Cell and Molecular Biology, Leibniz Institute for Natural Product Research and Infection Biology (Hans Knoell Institute), Beutenbergstrasse 11a, D-07745 Jena, Germany. Electronic address: frank.haenel@hki-jena.de.
authors.forename: Frank
authors.initials: F
authors.lastname: Hänel
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D002460:Cell Line; D002691:Chlamydophila psittaci / Q000502:physiology*; D004398:Dyneins / Q000378:metabolism; D054884:Host-Pathogen Interactions; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism*; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D011485:Protein Binding; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D020798:Two-Hybrid System Techniques; D033921:Vesicular Transport Proteins / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D008565:Membrane Proteins; C118519:SNAPIN protein, human; D033921:Vesicular Transport Proteins; D004398:Dyneins
keywords: Chlamydia; Dynein; Inc proteins; Interaction; Traffic