title: New cholinesterase inhibitory constituents from Lonicera quinquelocularis.
abstract: A phytochemical investigation on the ethyl acetate soluble fraction of Lonicera quinquelocularis (whole plant) led to the first time isolation of one new phthalate; bis(7-acetoxy-2-ethyl-5-methylheptyl) phthalate (3) and two new benzoates; neopentyl-4-ethoxy-3, 5-bis (3-methyl-2-butenyl benzoate (4) and neopentyl-4-hydroxy-3, 5-bis (3-methyl-2-butenyl benzoate (5) along with two known compounds bis (2-ethylhexyl phthalate (1) and dioctyl phthalate (2). Their structures were established on the basis of spectroscopic analysis and by comparison with available data in the literature. All the compounds (1-5) were tested for their acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitory activities in dose dependent manner. The IC50 (50% inhibitory effect) values of compounds 3 and 5 against AChE were 1.65 and 3.43 µM while the values obtained against BChE were 5.98 and 9.84 µM respectively. Compounds 2 and 4 showed weak inhibition profile.
authors.affiliation: Department of Chemistry, Gomal University, Dera Ismail Khan, Khyber Pakhtunkhwa, Pakistan.
authors.forename: Dilfaraz
authors.initials: D
authors.lastname: Khan
authors.affiliation: Departmet of Biotechnology, University of Science and Technology, Bannu, Khyber Pakhtunkhwa, Pakistan.
authors.forename: Hidayat Ullah
authors.initials: HU
authors.lastname: Khan
authors.affiliation: Department of Chemistry, University of Science and Technology, Bannu, Khyber Pakhtunkhwa, Pakistan.
authors.forename: Farmanullah
authors.initials: F
authors.lastname: Khan
authors.affiliation: Department of Chemistry, Gomal University, Dera Ismail Khan, Khyber Pakhtunkhwa, Pakistan.
authors.forename: Shafiullah
authors.initials: S
authors.lastname: Khan
authors.affiliation: Department of Chemistry, Gomal University, Dera Ismail Khan, Khyber Pakhtunkhwa, Pakistan.
authors.forename: Syed
authors.initials: S
authors.lastname: Badshah
authors.affiliation: Interdisciplinary Research Center in Biomedical Materials, COMSATS Institute of Information Technology, Lahore, Pakistan.
authors.forename: Abdul Samad
authors.initials: AS
authors.lastname: Khan
authors.affiliation: Department of Chemistry, Gomal University, Dera Ismail Khan, Khyber Pakhtunkhwa, Pakistan.
authors.forename: Abdul
authors.initials: A
authors.lastname: Samad
authors.affiliation: Department of Chemistry, Shaheed BBB University, Sheringal, Dir, Khyber Pakhtunkhwa, Pakistan.
authors.forename: Farman
authors.initials: F
authors.lastname: Ali
authors.affiliation: Department of Fundamental and Applied Sciences, Centre of Ionic Liquid, University of Technology Petronas, Perak, Malaysia.
authors.forename: Ihsanullah
authors.initials: I
authors.lastname: Khan
authors.affiliation: Interdisciplinary Research Center in Biomedical Materials, COMSATS Institute of Information Technology, Lahore, Pakistan.
authors.forename: Nawshad
authors.initials: N
authors.lastname: Muhammad
mesh_terms: D000818:Animals; D002091:Butyrylcholinesterase / Q000378:metabolism; D066241:Carbon-13 Magnetic Resonance Spectroscopy; D002800:Cholinesterase Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D002802:Cholinesterases / Q000378:metabolism*; D057075:Enzyme Assays; D006736:Horses; D020128:Inhibitory Concentration 50; D031239:Lonicera / Q000737:chemistry*; D066244:Proton Magnetic Resonance Spectroscopy
chemical_list: D002800:Cholinesterase Inhibitors; D002091:Butyrylcholinesterase; D002802:Cholinesterases
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