title: Development and characterization of lactoferrin loaded poly(epsilon-caprolactone) nanofibers.
abstract: Lactoferrin loaded poly (epsilon-caprolactone) nanofibers were fabricated using the process of electrospinning and the osteocompatibility of the scaffolds were evaluated using MC3T3-E1 osteoblast-like cells. Morphology of rhLF/PCL scaffolds was determined by scanning electron microscopy. Fourier transform infrared spectroscopy and Energy-dispersive X-ray spectroscopy confirmed the incorporation of rhLF in the PCL nanofibers. The surface distribution of rhLF on the nanofibers was evaluated using fluorescently tagged (Far-red) rhLF. The presence of rhLF on rhLF/PCL nanofibers significantly increased the proliferation and viability of MC3T3-E1 cells compared to cells seeded on PCL nanofibers as evidenced from Ki67 immuno-staining and MTT assay. The study demonstrated the feasibility of incorporating different concentrations of rhLF in PCL nanofibers and the potential of the fiber matrix to support osteoblast cell functions.
authors.forename: Eric N
authors.initials: EN
authors.lastname: James
authors.forename: Lakshmi S
authors.initials: LS
authors.lastname: Nair
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004337:Drug Carriers / Q000138:chemical synthesis* / Q000737:chemistry*; D004339:Drug Compounding / Q000379:methods; D007781:Lactoferrin / Q000737:chemistry* / Q000494:pharmacology; D008422:Materials Testing; D051379:Mice; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D011091:Polyesters / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry / Q000494:pharmacology; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D004337:Drug Carriers; D011091:Polyesters; D011994:Recombinant Proteins; C016240:polycaprolactone; D007781:Lactoferrin