title: Reversible cysteine oxidation in hydrogen peroxide sensing and signal transduction.
abstract: Activation of redox cascades through hydrogen peroxide-mediated reversible cysteine oxidation is a major mechanism for intracellular signaling. Understanding why some cysteine residues are specifically oxidized, in competition with other proximal cysteine residues and in the presence of strong redox buffers, is therefore crucial for understanding redox signaling. In this review, we explore the recent advances in thiol-redox chemistry linked to signaling. We describe the last findings in the field of redox sensors, those that are naturally present in different model organisms as well as those that have been engineered to quantify intracellular hydrogen peroxide concentrations. Finally, we provide a summary of the newest approaches developed to study reversible cysteine oxidation at the proteomic level.
authors.affiliation: Oxidative Stress and Cell Cycle Group, Departament de Ciències Experimentals i de la Salut, Universitat Pompeu Fabra , C/Dr. Aiguader 88, E-08003 Barcelona, Spain.
authors.forename: Sarela
authors.initials: S
authors.lastname: García-Santamarina
authors.forename: Susanna
authors.initials: S
authors.lastname: Boronat
authors.forename: Elena
authors.initials: E
authors.lastname: Hidalgo
mesh_terms: D015374:Biosensing Techniques / Q000379:methods; D003545:Cysteine / Q000737:chemistry / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism*; D010084:Oxidation-Reduction; D040901:Proteomics / Q000379:methods*; D017382:Reactive Oxygen Species; D015398:Signal Transduction; D013438:Sulfhydryl Compounds / Q000737:chemistry / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species; D013438:Sulfhydryl Compounds; D006861:Hydrogen Peroxide; D005978:Glutathione; D003545:Cysteine