title: Effect of methyl jasmonate on cadmium uptake and antioxidative capacity in Kandelia obovata seedlings under cadmium stress.
abstract: This study investigated the effects of methyl jasmonate (MeJA) on chlorophyll concentration, lipid peroxidation, Cd uptake, antioxidative capacity, and type-2 metallothionein gene (KoMT2) expression in the leaves of Kandelia obovata seedlings exposed to Cd stress. Deleterious effects, including decreased chlorophyll content and increased malondialdehyde concentration, were observed in leaves of K. obovata after 9d of 200μmol L(-1) Cd treatment. Application of MeJA (0.1 to 1μmol L(-1)) increased the concentration of ascorbic acid and the activities of catalase and ascorbate peroxidase in the leaves of K. obovata, which helped alleviate the oxidative damage induced by Cd stress. The concentration of endogenous jasmonic acid in the leaves of K. obovata was decreased by Cd but was positively stimulated by exogenous MeJA. The expression of KoMT2 in the leaves was enhanced after 9d of 200μmol L(-1) Cd treatment, while the exogenous application of MeJA significantly restored the expression of KoMT2. Exogenous MeJA also inhibited the uptake of Cd to the aboveground part (leaves) of the seedlings, which helped reduce direct damages of Cd to the photosynthetic organ of the plant. The reduced uptake of Cd might be a result of stomatal closure and decreased transpiration by exogenous MeJA.
authors.affiliation: State Key Laboratory of Estuarine and Coastal Research, East China Normal University, Shanghai, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Estuarine and Coastal Research, East China Normal University, Shanghai, China. Electronic address: yanzz.gz@gmail.com.
authors.forename: Zhongzheng
authors.initials: Z
authors.lastname: Yan
authors.affiliation: State Key Laboratory of Estuarine and Coastal Research, East China Normal University, Shanghai, China. Electronic address: xzli@sklec.ecnu.edu.cn.
authors.forename: Xiuzhen
authors.initials: X
authors.lastname: Li
mesh_terms: D000085:Acetates / Q000494:pharmacology*; D002104:Cadmium / Q000378:metabolism* / Q000633:toxicity; D003517:Cyclopentanes / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D004798:Enzymes / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D015227:Lipid Peroxidation / Q000187:drug effects; D008315:Malondialdehyde / Q000032:analysis / Q000378:metabolism; D008668:Metallothionein / Q000032:analysis / Q000235:genetics; D054883:Oxylipins / Q000494:pharmacology*; D010937:Plant Growth Regulators / Q000494:pharmacology; D018515:Plant Leaves / Q000187:drug effects / Q000201:enzymology; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism; D043982:Rhizophoraceae / Q000187:drug effects* / Q000378:metabolism; D036226:Seedlings / Q000187:drug effects* / Q000378:metabolism; D012989:Soil Pollutants / Q000378:metabolism / Q000633:toxicity; D013312:Stress, Physiological / Q000187:drug effects
chemical_list: D000085:Acetates; D003517:Cyclopentanes; D004798:Enzymes; D054883:Oxylipins; D010937:Plant Growth Regulators; D012989:Soil Pollutants; D002104:Cadmium; D008315:Malondialdehyde; C072239:methyl jasmonate; D008668:Metallothionein
keywords: Heavy metals; Jasmonic acid; Mangrove; Metallothionein; Stress