title: Involvement of satellite I noncoding RNA in regulation of chromosome segregation.
abstract: Human centromeres consist of repetitive sequences from which satellite I noncoding RNAs are transcribed. We found that knockdown of satellite I RNA causes abnormal chromosome segregation and generation of nuclei with a grape-shape phenotype. Co-immunoprecipitation experiments showed that satellite I RNA associates with Aurora B, a component of the chromosome passenger complex (CPC) regulating proper attachment of microtubules to kinetochores, in mitotic HeLa cells. Satellite I RNA was also shown to associate with INCENP, another component of the CPC. In addition, depletion of satellite I RNA resulted in up-regulation of kinase activity of Aurora B and delocalization of the CPC from the centromere region. These results suggest that satellite I RNA is involved in chromosome segregation through controlling activity and centromeric localization of Aurora B kinase.
authors.affiliation: Department of Biological Sciences, Graduate School of Science Technology, Kumamoto University, Kumamoto, 860-8555, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Ideue
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Cho
authors.forename: Kanako
authors.initials: K
authors.lastname: Nishimura
authors.forename: Tokio
authors.initials: T
authors.lastname: Tani
mesh_terms: D064107:Aurora Kinase B / Q000235:genetics / Q000378:metabolism; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism; D048750:Cell Nucleus Division; D002503:Centromere / Q000235:genetics / Q000378:metabolism; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics / Q000378:metabolism; D020090:Chromosome Segregation / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D007399:Interphase; D022661:RNA, Untranslated / Q000378:metabolism*
chemical_list: D002868:Chromosomal Proteins, Non-Histone; C083767:INCENP protein, human; D022661:RNA, Untranslated; C576921:AURKB protein, human; D064107:Aurora Kinase B