title: Multiplex lateral flow immunoassay for mycotoxin determination.
abstract: A new lateral flow immunoassay (LFA) is proposed for qualitative and/or semiquantitative determination of aflatoxin B1 (AFB1), zearalenone (ZEA), deoxynivalenol (DON), and their analogues (AFs, ZEAs, DONs) in cereal samples. Each of the mycotoxin specific antibody was class specific and there was no cross reactivity to other groups of compounds. The visual limits of detection (vLOD) of the strip were 0.03, 1.6, and 10 μg/kg for AFB1, ZEA and DON, respectively. The calculated limits of detection (cLOD) were 0.05, 1, and 3 μg/kg, respectively. Meanwhile the cutoff values were achieved at 1, 50, and 60 μg/kg for AFB1, ZEA and DON, respectively. Recoveries ranged from 80% to 122% and RSD from 5% to 20%. Both the vLOD and cLOD for the three mycotoxins were lower than the EU maximum levels. Analysis of naturally contaminated maize samples resulted in a good agreement between the multiplex LFA and LC-MS/MS (100% for DONs and AFs, and 81% for ZEAs). Careful analysis of the results further explained the general overestimation of LFA compared to chromatographic methods for quantification of mycotoxins.
authors.affiliation: Institute for Agro-food Standards and Testing Technology, Laboratory of Quality & Safety Risk Assessment for Agro-products (Shanghai), Ministry of Agriculture, Shanghai Academy of Agricultural Sciences , 1000 Jinqi Road, Shanghai 201403, China.
authors.forename: Suquan
authors.initials: S
authors.lastname: Song
authors.forename: Na
authors.initials: N
authors.lastname: Liu
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Zhao
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Njumbe Ediage
authors.forename: Songling
authors.initials: S
authors.lastname: Wu
authors.forename: Changpo
authors.initials: C
authors.lastname: Sun
authors.forename: Sarah
authors.initials: S
authors.lastname: De Saeger
authors.forename: Aibo
authors.initials: A
authors.lastname: Wu
mesh_terms: D016604:Aflatoxin B1 / Q000032:analysis; D000918:Antibody Specificity; D007118:Immunoassay / Q000379:methods*; D057230:Limit of Detection; D009183:Mycotoxins / Q000032:analysis*; D053719:Tandem Mass Spectrometry; D014255:Trichothecenes / Q000032:analysis; D014908:Triticum / Q000737:chemistry; D003313:Zea mays / Q000737:chemistry; D015025:Zearalenone / Q000032:analysis
chemical_list: D009183:Mycotoxins; D014255:Trichothecenes; D015025:Zearalenone; D016604:Aflatoxin B1; C007262:deoxynivalenol