title: Possibilities of optical imaging of the (99m)Tc-based radiopharmaceuticals.
abstract: In vivo optical imaging is widely used in preclinical studies. Recently, the application of optical imaging systems for preclinical visualization of gamma-emitting isotopes has become of interest since the evaluation of various organs relies on (99m)Tc-based radiopharmaceuticals (RPs). In vitro radioluminescence of (99m)Tc-based RPs, including pertechnetate, albumin macroaggregates, dimercaptosuccinic acid, phytate colloid, and ethylenediamine tetramethylene phosphonic acid, was studied with IVIS Spectrum CT™ optical imaging system. The distribution of phytate colloid was also studied in vivo with and without scintillating materials and the results were compared with those obtained with a conventional scintigraphy. The visible light emission appeared to be due to the radioluminescence of water and luminophores contained in RPs rather than from Cherenkov radiation. Weak air luminescence affected the background. The radioluminescence of fluids induced by (99m)Tc-based tracers could be detected using charge-coupled device optical imaging systems. The radioluminescence intensity and its spectral distribution depend on the surrounding fluid and known luminophores present. Thus, in some cases the in vivo optical imaging is possible but the use of scintillator, e.g., borosilicate glass or bismuth germanate, is preferred.
authors.affiliation: Pirogov Russian National Research Medical University, Department of Radiology and Radiotherapy, 1 Ostrovityanova Street, Moscow 117997, Russian Federation.
authors.forename: Anton K
authors.initials: AK
authors.lastname: Kondakov
authors.affiliation: Pirogov Russian National Research Medical University, Department of Medical Nanobiotechnology, 1 Ostrovityanova Street, Moscow 117997, Russian Federation.
authors.forename: Ilya L
authors.initials: IL
authors.lastname: Gubskiy
authors.affiliation: Pirogov Russian National Research Medical University, Department of Radiology and Radiotherapy, 1 Ostrovityanova Street, Moscow 117997, Russian FederationcCentral Clinical Hospital of the Russian Academy of Sciences, 12 b.3 Fotievoy Street, Moscow 119333.
authors.forename: Igor A
authors.initials: IA
authors.lastname: Znamenskiy
authors.affiliation: Pirogov Russian National Research Medical University, Department of Medical Nanobiotechnology, 1 Ostrovityanova Street, Moscow 117997, Russian FederationdSerbsky State Research Center of Social and Forensic Psychiatry, Division of Fundamental and Applied.
authors.forename: Vladimir P
authors.initials: VP
authors.lastname: Chekhonin
mesh_terms: D000818:Animals; D051379:Mice; D008962:Models, Theoretical; D061848:Optical Imaging / Q000379:methods*; D019275:Radiopharmaceuticals / Q000737:chemistry* / Q000493:pharmacokinetics; D051381:Rats; D017208:Rats, Wistar; D017556:Technetium Compounds / Q000737:chemistry* / Q000493:pharmacokinetics
chemical_list: D019275:Radiopharmaceuticals; D017556:Technetium Compounds