title: Expression of S100A6 in rat hippocampus after traumatic brain injury due to lateral head acceleration.
abstract: In a rat model of traumatic brain injury (TBI), we investigated changes in cognitive function and S100A6 expression in the hippocampus. TBI-associated changes in this protein have not previously been reported. Rat S100A6 was studied via immunohistochemical staining, Western blot, and reverse transcription-polymerase chain reaction (RT-PCR) after either lateral head acceleration or sham. Reduced levels of S100A6 protein and mRNA were observed 1 h after TBI, followed by gradual increases over 6, 12, 24, and 72 h, and then a return to sham level at 14 day. Morris water maze (MWM) test was used to evaluate animal spatial cognition. TBI- and sham-rats showed an apparent learning curve, expressed as escape latency. Although TBI-rats displayed a relatively poorer cognitive ability than sham-rats, the disparity was not significant early post-injury. Marked cognitive deficits in TBI-rats were observed at 72 h post-injury compared with sham animals. TBI-rats showed decreased times in platform crossing in the daily MWM test; the performance at 72 h post-injury was the worst. In conclusion, a reduction in S100A6 may be one of the early events that lead to secondary cognitive decline after TBI, and its subsequent elevation is tightly linked with cognitive improvement. S100A6 may play important roles in neuronal degeneration and regeneration in TBI.
authors.affiliation: Department of Neurosurgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China. happyfangbo@gmail.com.
authors.forename: Bo
authors.initials: B
authors.lastname: Fang
authors.affiliation: Department of Neurosurgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China. liangming777@163.com.
authors.forename: Ming
authors.initials: M
authors.lastname: Liang
authors.affiliation: Department of Medical, Teaching and Research Administration, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China. guitaoy@hotmail.com.
authors.forename: Guitao
authors.initials: G
authors.lastname: Yang
authors.affiliation: Department of Neurosurgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China. chinayeyuqin@163.com.
authors.forename: Yuqin
authors.initials: Y
authors.lastname: Ye
authors.affiliation: Department of Neurosurgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China. xuhy@fmmu.edu.cn.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Xu
authors.affiliation: Department of Neurosurgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China. hexiaos@fmmu.edu.cn.
authors.forename: Xiaosheng
authors.initials: X
authors.lastname: He
authors.affiliation: Department of Neurosurgery, University of Rochester, Rochester, NY 14642, USA. jason_huang@urmc.rochester.edu.
authors.forename: Jason H
authors.initials: JH
authors.lastname: Huang
mesh_terms: D000818:Animals; D001522:Behavior, Animal; D001930:Brain Injuries / Q000378:metabolism* / Q000503:physiopathology*; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000378:metabolism*; D005786:Gene Expression Regulation; D006624:Hippocampus / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D000075383:S100 Calcium Binding Protein A6; D009418:S100 Proteins / Q000235:genetics* / Q000378:metabolism*; D013997:Time Factors
chemical_list: D018797:Cell Cycle Proteins; D012333:RNA, Messenger; D000075383:S100 Calcium Binding Protein A6; D009418:S100 Proteins; C489132:S100a6 protein, rat
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