title: Plasma microRNA-320, microRNA-let-7e and microRNA-21 as novel potential biomarkers for the detection of retinoblastoma.
abstract: Retinoblastoma (RB) is a childhood malignancy caused by inactivation of theRBgene, with neuron-specific enolase (NSE) levels considered as its diagnostic marker. MicroRNAs (miRNAs) have been proven to play a significant role in multiple physiological and pathological processes and several miRNAs were identified as tumor biomarkers in recent studies. In the present study, 65 plasma samples were collected from RB patients and 65 samples from healthy individuals to serve as controls. The miRNA levels were measured via quantitative reverse transcription-polymerase chain reaction and their association with RB was assessed by statistical data analysis and receiver operating characteristic curves. Plasma miRNA (miR)-320, miR-let-7e and miR-21 levels were downregulated in the patient samples, the areas under the curves (AUCs) were 0.548-0.660, whereas the AUCs of combined classifiers were ≥0.990. The plasma miRNA levels, particularly of miR-320, were found to be of value in RB diagnosis and may be considered as novel diagnostic biomarkers.
authors.affiliation: Graduate Division, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China ; Department of Pediatrics, General Hospital of Chinese People's Armed Police Forces, Beijing 100039, P.R. China.
authors.forename: Shan-Shan
authors.initials: SS
authors.lastname: Liu
authors.affiliation: Beijing Institute of Radiation Medicine, Beijing 100850, P.R. China ; College of Life Sciences, Jilin University, Changchun, Jilin 130012, P.R. China.
authors.forename: Yu-Shi
authors.initials: YS
authors.lastname: Wang
authors.affiliation: Department of Pediatrics, General Hospital of Chinese People's Armed Police Forces, Beijing 100039, P.R. China.
authors.forename: Yan-Feng
authors.initials: YF
authors.lastname: Sun
authors.affiliation: Department of Pediatrics, General Hospital of Chinese People's Armed Police Forces, Beijing 100039, P.R. China.
authors.forename: Li-Xia
authors.initials: LX
authors.lastname: Miao
authors.affiliation: Department of Pediatrics, General Hospital of Chinese People's Armed Police Forces, Beijing 100039, P.R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Pediatrics, General Hospital of Chinese People's Armed Police Forces, Beijing 100039, P.R. China.
authors.forename: Yan-Shan
authors.initials: YS
authors.lastname: Li
authors.affiliation: Department of Pediatrics, General Hospital of Chinese People's Armed Police Forces, Beijing 100039, P.R. China.
authors.forename: Hong-Yan
authors.initials: HY
authors.lastname: Liu
authors.affiliation: Department of Pediatrics, General Hospital of Chinese People's Armed Police Forces, Beijing 100039, P.R. China.
authors.forename: Qiu-Ling
authors.initials: QL
authors.lastname: Liu
keywords: biomarker; microRNA; plasma; retinoblastoma
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