title: LSPR chip for parallel, rapid, and sensitive detection of cancer markers in serum.
abstract: Label-free biosensing based on metallic nanoparticles supporting localized surface plasmon resonances (LSPR) has recently received growing interest (Anker, J. N., et al. Nat. Mater. 2008, 7, 442-453). Besides its competitive sensitivity (Yonzon, C. R., et al. J. Am. Chem. Soc. 2004, 126, 12669-12676; Svendendahl, M., et al. Nano Lett. 2009, 9, 4428-4433) when compared to the surface plasmon resonance (SPR) approach based on extended metal films, LSPR biosensing features a high-end miniaturization potential and a significant reduction of the interrogation device bulkiness, positioning itself as a promising candidate for point-of-care diagnostic and field applications. Here, we present the first, paralleled LSPR lab-on-a-chip realization that goes well beyond the state-of-the-art, by uniting the latest advances in plasmonics, nanofabrication, microfluidics, and surface chemistry. Our system offers parallel, real-time inspection of 32 sensing sites distributed across 8 independent microfluidic channels with very high reproducibility/repeatability. This enables us to test various sensing strategies for the detection of biomolecules. In particular we demonstrate the fast detection of relevant cancer biomarkers (human alpha-feto-protein and prostate specific antigen) down to concentrations of 500 pg/mL in a complex matrix consisting of 50% human serum.
authors.affiliation: ICFO - Institut de Ciencies Fotoniques , Mediterranean Technology Park, 08860 Castelldefels (Barcelona), Spain.
authors.forename: Srdjan S
authors.initials: SS
authors.lastname: Aćimović
authors.forename: Maria A
authors.initials: MA
authors.lastname: Ortega
authors.forename: Vanesa
authors.initials: V
authors.lastname: Sanz
authors.forename: Johann
authors.initials: J
authors.lastname: Berthelot
authors.forename: Jose L
authors.initials: JL
authors.lastname: Garcia-Cordero
authors.forename: Jan
authors.initials: J
authors.lastname: Renger
authors.forename: Sebastian J
authors.initials: SJ
authors.lastname: Maerkl
authors.forename: Mark P
authors.initials: MP
authors.lastname: Kreuzer
authors.forename: Romain
authors.initials: R
authors.lastname: Quidant
mesh_terms: D014408:Biomarkers, Tumor / Q000097:blood*; D015374:Biosensing Techniques; D006801:Humans; D056656:Lab-On-A-Chip Devices; D053768:Metal Nanoparticles / Q000737:chemistry; D046210:Microfluidic Analytical Techniques; D009369:Neoplasms / Q000097:blood*; D017430:Prostate-Specific Antigen / Q000097:blood*; D020349:Surface Plasmon Resonance; D000509:alpha-Fetoproteins
chemical_list: D014408:Biomarkers, Tumor; D000509:alpha-Fetoproteins; D017430:Prostate-Specific Antigen