title: Live-cell stimulated Raman scattering imaging of alkyne-tagged biomolecules.
abstract: Alkynes can be metabolically incorporated into biomolecules including nucleic acids, proteins, lipids, and glycans. In addition to the clickable chemical reactivity, alkynes possess a unique Raman scattering within the Raman-silent region of a cell. Coupling this spectroscopic signature with Raman microscopy yields a new imaging modality beyond fluorescence and label-free microscopies. The bioorthogonal Raman imaging of various biomolecules tagged with an alkyne by a state-of-the-art Raman imaging technique, stimulated Raman scattering (SRS) microscopy, is reported. This imaging method affords non-invasiveness, high sensitivity, and molecular specificity and therefore should find broad applications in live-cell imaging.
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Department of Chemical Biology, College of Chemistry and Molecular Engineering, Synthetic and Functional Biomolecules Center, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871 (China).
authors.forename: Senlian
authors.initials: S
authors.lastname: Hong
authors.forename: Tao
authors.initials: T
authors.lastname: Chen
authors.forename: Yuntao
authors.initials: Y
authors.lastname: Zhu
authors.forename: Ang
authors.initials: A
authors.lastname: Li
authors.forename: Yanyi
authors.initials: Y
authors.lastname: Huang
authors.forename: Xing
authors.initials: X
authors.lastname: Chen
mesh_terms: D000480:Alkynes / Q000737:chemistry*; D003952:Diagnostic Imaging / Q000295:instrumentation*; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D000480:Alkynes
keywords: Raman imaging; biomolecules; bioorthogonality; stimulated Raman scattering