title: Identification and genetic characterization of porcine hemagglutinating encephalomyelitis virus from domestic piglets in China.
abstract: In this study, we investigated an acute outbreak of porcine hemagglutinating encephalomyelitis on a farm of 127 pigs in Jilin province, China. Porcine hemagglutinating encephalomyelitis virus (PHEV) was detected in suckling and weaning pigs by RT-PCR assays. Coronavirus-like particles were observed by electron microscopy. The virus isolate was designated PHEV-JT06. The clinical signs, nervous symptoms and positive labeling of neurons in the cerebral cortex with an immunohistochemical stain in PHEV-JT06-infected BALB/c mice supported the diagnosis of PHEV infection. The five full-length PHEV-JT06 structural genes were cloned, sequenced and analyzed. Phylogenetic studies based on the nucleotide and amino acid sequences of the five genes in the outbreak showed that PHEV remained genetically stable. PHEV shares 95.3-99.3% amino acid sequence identity with American strains (AY078417), suggesting that the Chinese isolate is most likely derived from the North American strain. Additionally, PHEV, HCoV-OC43 and BCoV were genetically close. These results may provide some insights into the genotype of the etiological agent responsible for the porcine hemagglutinating encephalomyelitis outbreak and could also provide a comparative view of the genomics of the five structural proteins of PHEV.
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, 130062, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Dong
authors.forename: Huijun
authors.initials: H
authors.lastname: Lu
authors.forename: Kui
authors.initials: K
authors.lastname: Zhao
authors.forename: Wenfeng
authors.initials: W
authors.lastname: Liu
authors.forename: Wei
authors.initials: W
authors.lastname: Gao
authors.forename: Yungang
authors.initials: Y
authors.lastname: Lan
authors.forename: Jiakuan
authors.initials: J
authors.lastname: Zhao
authors.forename: Bo
authors.initials: B
authors.lastname: Tang
authors.forename: Deguang
authors.initials: D
authors.lastname: Song
authors.forename: Wenqi
authors.initials: W
authors.lastname: He
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002681:China / Q000453:epidemiology; D003001:Cloning, Molecular; D016000:Cluster Analysis; D017934:Coronavirus / Q000302:isolation & purification*; D018352:Coronavirus Infections / Q000453:epidemiology* / Q000473:pathology / Q000821:virology; D004195:Disease Models, Animal; D004196:Disease Outbreaks; D005260:Female; D014644:Genetic Variation; D006651:Histocytochemistry; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D034421:Sus scrofa; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology* / Q000473:pathology / Q000821:virology; D014764:Viral Proteins / Q000235:genetics
chemical_list: D014764:Viral Proteins
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