title: HIV-1 Rev protein specifies the viral RNA export pathway by suppressing TAP/NXF1 recruitment.
abstract: Nuclear RNA export pathways in eukaryotes are often linked to the fate of a given RNA. Therefore, the choice of export pathway should be well-controlled to avoid an unfavorable effect on gene expression. Although some RNAs could be exported by more than one pathway, little is known about how the choice is regulated. This issue is highlighted when the human immunodeficiency virus type 1 (HIV-1) Rev protein induces the export of singly spliced and unspliced HIV-1 transcripts. How these RNAs are exported is not well understood because such transcripts should have the possibility of utilizing CRM1-dependent export via Rev or cellular TAP/NXF1-dependent export via the transcription/export (TREX) complex, or both. Here we found that Rev suppressed TAP/NXF1-dependent export of model RNA substrates that recapitulated viral transcripts. In this effect, Rev interacted with the cap-binding complex and inhibited the recruitment of the TREX complex. Thus, Rev controls the identity of the factor occupying the cap-proximal region that determines the RNA export pathway. This ribonucleoprotein remodeling activity of Rev may favor viral gene expression.
authors.affiliation: Institute for Virus Research, Kyoto University, Kyoto 606-8507, Japan itaniguc@virus.kyoto-u.ac.jp.
authors.forename: Ichiro
authors.initials: I
authors.lastname: Taniguchi
authors.affiliation: Institute for Virus Research, Kyoto University, Kyoto 606-8507, Japan.
authors.forename: Naoto
authors.initials: N
authors.lastname: Mabuchi
authors.affiliation: Institute for Virus Research, Kyoto University, Kyoto 606-8507, Japan hitoohno@virus.kyoto-u.ac.jp.
authors.forename: Mutsuhito
authors.initials: M
authors.lastname: Ohno
mesh_terms: D000818:Animals; D029721:Drosophila Proteins / Q000235:genetics; D051717:Fushi Tarazu Transcription Factors / Q000235:genetics; D015497:HIV-1 / Q000235:genetics* / Q000378:metabolism; D028884:Karyopherins / Q000378:metabolism; D029543:Nucleocytoplasmic Transport Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D009865:Oocytes / Q000378:metabolism; D039381:RNA Cap-Binding Proteins / Q000378:metabolism; D012326:RNA Splicing; D034443:RNA Transport; D012367:RNA, Viral / Q000737:chemistry / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism*; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism; D014981:Xenopus; D054321:rev Gene Products, Human Immunodeficiency Virus / Q000378:metabolism*; D000097606:Exportin 1 Protein
chemical_list: D029721:Drosophila Proteins; D051717:Fushi Tarazu Transcription Factors; D028884:Karyopherins; D029543:Nucleocytoplasmic Transport Proteins; D039381:RNA Cap-Binding Proteins; D012367:RNA, Viral; D016601:RNA-Binding Proteins; D018160:Receptors, Cytoplasmic and Nuclear; D054321:rev Gene Products, Human Immunodeficiency Virus; D000097606:Exportin 1 Protein; C497169:ftz protein, Drosophila; C514860:rev protein, Human Immunodeficiency Virus-1
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