title: Effect of trapidil in myocardial ischemia-reperfusion injury in rabbit.
abstract: OBJECTIVE
  To evaluate the cardioprotective effects of trapidil on myocardial ischemia-reperfusion injury (MIRI) in rabbits.
  
  
  METHODS
  Rabbits were subjected to 40 min of myocardial ischemia followed by 120 min of reperfusion. Blood for superoxide dismutase (SOD) and malondialdehyde (MDA) were estimated. At the end of reperfusion, the rabbits were sacrificed and the hearts were isolated for histological examination. An apoptotic index (AI) was determined using the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick-end-labeling (TUNEL) method. The expression of apoptosis-related proteins Bax and Bcl-2 was analyzed using immunohistochemistry. Statistical analyses were performed by one-way analysis of variance (ANOVA), P < 0.05 considered statistically significant.
  
  
  RESULTS
  Trapidil caused a significant (P < 0.05) increase in SOD activity, as decreased MDA levels and significantly (P < 0.05) reduced the expression of Bax as compared with the ischemia-reperfusion (IR) control group.
  
  
  CONCLUSIONS
  Trapidil may attenuate the myocardial damage produced by IR injury and offer potential cardioprotective action.
authors.affiliation: The First Hospital of Jilin University, Changchun, Jilin 130000, China ; Inner Mongolia University for the Nationalities, Tongliao, Inner Mongolia 028000, China.
authors.forename: Mingjie
authors.initials: M
authors.lastname: Liu
authors.affiliation: The First Hospital of Jilin University, Changchun, Jilin 130000, China ; 202 Military Hospital of China, Shenyang, Liaoning 110000, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Sun
authors.affiliation: 202 Military Hospital of China, Shenyang, Liaoning 110000, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Liaoning Provincial Institute of Food and Drug Control, Shenyang, Liaoning 110000, China.
authors.forename: Xiuying
authors.initials: X
authors.lastname: Wang
authors.affiliation: Liaoning Provincial Institute of Food and Drug Control, Shenyang, Liaoning 110000, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Lin
authors.affiliation: Liaoning Provincial Institute of Food and Drug Control, Shenyang, Liaoning 110000, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Yang
authors.affiliation: Liaoning Provincial Institute of Food and Drug Control, Shenyang, Liaoning 110000, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Yan
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects; D002316:Cardiotonic Agents / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D008297:Male; D008315:Malondialdehyde / Q000097:blood / Q000378:metabolism; D046529:Microscopy, Electron, Transmission; D015428:Myocardial Reperfusion Injury / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D009206:Myocardium / Q000378:metabolism / Q000648:ultrastructure; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D011817:Rabbits; D013482:Superoxide Dismutase / Q000097:blood / Q000378:metabolism; D014192:Trapidil / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D000975:Antioxidants; D002316:Cardiotonic Agents; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D014192:Trapidil
keywords: Apoptosis index; ischemia-reperfusion; myocardial ischemia-reperfusion injury; trapidil
references.citation: Int J Biol Macromol. 2010 Apr 1;46(3):363-6
references.pmid: 20083132
references.citation: J Transl Med. 2011 May 21;9:73
references.pmid: 21600015
references.citation: Indian J Pharmacol. 2012 Jan;44(1):68-72
references.pmid: 22345873
references.citation: Cell Biochem Biophys. 2011 Jul;60(3):249-58
references.pmid: 21210248
references.citation: J Pharmacol Exp Ther. 2011 Jul;338(1):337-44
references.pmid: 21493751
references.citation: J Renin Angiotensin Aldosterone Syst. 2009 Dec;10(4):201-9
references.pmid: 20026869
references.citation: Chin Med J (Engl). 2010 Dec;123(24):3597-604
references.pmid: 22166638
references.citation: Chin Med J (Engl). 2011 Jun;124(11):1683-8
references.pmid: 21740777
references.citation: Indian J Pharm Sci. 2010 Nov;72(6):745-52
references.pmid: 21969747
references.citation: Tissue Cell. 2012 Oct;44(5):308-15
references.pmid: 22658447
references.citation: J Pharmacol Sci. 2012;119(3):251-9
references.pmid: 22785021
references.citation: Eur J Pharmacol. 2005 May 16;515(1-3):28-33
references.pmid: 15894306
references.citation: Semin Cardiothorac Vasc Anesth. 2011 Mar-Jun;15(1-2):56-65
references.pmid: 21719546
references.citation: Peptides. 2012 Feb;33(2):329-35
references.pmid: 22244813
references.citation: Int J Nanomedicine. 2011;6:1391-8
references.pmid: 21760733
references.citation: Int J Med Sci. 2011;8(7):564-72
references.pmid: 21960749
references.citation: Eur J Pharmacol. 2010 Jul 25;638(1-3):84-9
references.pmid: 20406632
references.citation: J Cell Biochem. 2008 May 1;104(1):119-28
references.pmid: 17979183
references.citation: Gene. 2013 Sep 15;527(1):389-93
references.pmid: 23727604