title: The effects of two polymeric matrices for indomethacin in cutaneous nociceptive reactivity in mice.
abstract: OBJECTIVE
  This paper is focused on the investigation the effects of two polymeric matrices for indomethacin in cutaneous pain models in mice. There were used two different co-polymers polyhydroxyethyl methacrylate and undecan, polymerization reactions being conducted under nitrogen, at 80 degrees C.
  
  
  METHODS
  The experiments were carried out on white Swiss mice (20-25 g), divided into 6 groups of 7 animals each, treated orally. Biocompatibility properties of indomethacin-loaded copolymeric matrices ware evaluated by assessing the effects on the blood parameters, the serum biochemical tests of animals treated. The nociceptive somatic testing was performed using hot plate assay and tail immersion test. The latency (second) response to paw, respectively tail thermal noxious stimulation, was measured before the experiment and 15, 30, 60, 90, 120 minutes, 4, 6, 8, 10, 12 hours after the substances administration.
  
  
  RESULTS
  Laboratory analysis did not show significant differences of blood parameters, serum biochemical tests between control mice group (IND) and groups treated with 1 M, 1 IND, 3 M, 3 IND. In our experimental conditions IND determined a significant increasing of the latency period response, in hot plate and also in tail immersion tests. Using two different co-polymers for indomethacin incorporation we obtained an increasing of the latency time pain reaction in hot plate assay, respectively in tail immersion test, statistically significant (*p < 0.05) compared with the simple copolymers administration.
  
  
  CONCLUSIONS
  We demonstrated that indomethacin co-polymeric matrices 1 IND and 3 IND determined similar immune responses with indomethacin and simple co-polymers after oral administration in mice, indicative of good in vivo biocompatibility. Oral administration of both 1 IND and 3 IND resulted in prolonged antinociceptive effects in hot plate assay and also in tail immersion test in mice.
authors.forename: Mihaela
authors.initials: M
authors.lastname: Boancă
authors.forename: Aurica
authors.initials: A
authors.lastname: Chiriac
authors.forename: Loredana
authors.initials: L
authors.lastname: Niţă
authors.forename: Otilia
authors.initials: O
authors.lastname: Novac
authors.forename: Cătălina Elena
authors.initials: CE
authors.lastname: Lupuşoru
authors.forename: V
authors.initials: V
authors.lastname: Poroch
authors.forename: Liliana
authors.initials: L
authors.lastname: Tarţău
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D003080:Cold Temperature; D004195:Disease Models, Animal; D004337:Drug Carriers; D004339:Drug Compounding; D006358:Hot Temperature; D007213:Indomethacin / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D051379:Mice; D008815:Mice, Inbred Strains; D010146:Pain / Q000209:etiology / Q000517:prevention & control*; D010147:Pain Measurement / Q000187:drug effects / Q000295:instrumentation / Q000379:methods; D017288:Pain Threshold / Q000187:drug effects; D011102:Polyhydroxyethyl Methacrylate; D011108:Polymers
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D004337:Drug Carriers; D011108:Polymers; D011102:Polyhydroxyethyl Methacrylate; D007213:Indomethacin