title: Zebrafish models for translational neuroscience research: from tank to bedside.
abstract: The zebrafish (Danio rerio) is emerging as a new important species for studying mechanisms of brain function and dysfunction. Focusing on selected central nervous system (CNS) disorders (brain cancer, epilepsy, and anxiety) and using them as examples, we discuss the value of zebrafish models in translational neuroscience. We further evaluate the contribution of zebrafish to neuroimaging, circuit level, and drug discovery research. Outlining the role of zebrafish in modeling a wide range of human brain disorders, we also summarize recent applications and existing challenges in this field. Finally, we emphasize the potential of zebrafish models in behavioral phenomics and high-throughput genetic/small molecule screening, which is critical for CNS drug discovery and identifying novel candidate genes.
authors.affiliation: ZENEREI Institute and the International Zebrafish Neuroscience Research Consortium (ZNRC), 309 Palmer Court, Slidell, LA 70458, USA; Department of Neuroscience, University of Pittsburgh, A210 Langley Hall, Pittsburgh, PA 15260, USA.
authors.forename: Adam Michael
authors.initials: AM
authors.lastname: Stewart
authors.affiliation: Center for Functional Connectomics, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seoul, 136791, Republic of Korea.
authors.forename: Oliver
authors.initials: O
authors.lastname: Braubach
authors.affiliation: Department of Microbiology, Oregon State University, Nash Hall 220 Corvallis, OR 97331, USA.
authors.forename: Jan
authors.initials: J
authors.lastname: Spitsbergen
authors.affiliation: Department of Psychology, University of Toronto at Mississauga, 3359 Mississauga Road, N Mississauga, Ontario L5L 1C6, Canada.
authors.forename: Robert
authors.initials: R
authors.lastname: Gerlai
authors.affiliation: ZENEREI Institute and the International Zebrafish Neuroscience Research Consortium (ZNRC), 309 Palmer Court, Slidell, LA 70458, USA. Electronic address: avkalueff@gmail.com.
authors.forename: Allan V
authors.initials: AV
authors.lastname: Kalueff
mesh_terms: D000818:Animals; D001927:Brain Diseases / Q000628:therapy; D004195:Disease Models, Animal; D006801:Humans; D009488:Neurosciences; D057170:Translational Research, Biomedical / Q000379:methods*; D015027:Zebrafish
keywords: anxiety; biomarkers; brain disorders; cancer; epilepsy; zebrafish
references.citation: Behav Brain Res. 2010 Mar 5;207(2):223-31
references.pmid: 19836422
references.citation: Behav Brain Res. 2013 Mar 1;240:192-6
references.pmid: 23219966
references.citation: Proc Natl Acad Sci U S A. 2011 Feb 8;108(6):2581-6
references.pmid: 21262817
references.citation: Mol Psychiatry. 2013 Jun;18(6):681-91
references.pmid: 22641177
references.citation: PLoS One. 2011;6(8):e24185
references.pmid: 21912623
references.citation: Brain Res. 1999 Jul 17;835(1):18-26
references.pmid: 10448192
references.citation: Behav Brain Res. 2010 Jun 26;210(1):1-7
references.pmid: 20117146
references.citation: Prog Neuropsychopharmacol Biol Psychiatry. 2014 Dec 3;55:1-6
references.pmid: 24593944
references.citation: Neuroscience. 2013 Aug 15;245:136-47
references.pmid: 23618759
references.citation: Behav Brain Res. 2007 Apr 16;179(1):1-18
references.pmid: 17306892
references.citation: Neurosci Biobehav Rev. 2007;31(6):882-96
references.pmid: 17706782
references.citation: Behav Brain Res. 2010 Mar 17;208(1):56-62
references.pmid: 19896505
references.citation: J Neurosci Methods. 2012 Sep 30;210(2):266-71
references.pmid: 22884772
references.citation: Behav Brain Res. 2012 Jun 15;232(1):264-8
references.pmid: 22561034
references.citation: Behav Brain Res. 2013 Nov 1;256:172-87
references.pmid: 23948218
references.citation: Neurochem Res. 2011 Oct;36(10):1876-86
references.pmid: 21603935
references.citation: Horm Behav. 1997 Jun;31(3):197-211
references.pmid: 9213134
references.citation: Zebrafish. 2013 Mar;10(1):70-86
references.pmid: 23590400
references.citation: Neurotoxicol Teratol. 2011 Nov-Dec;33(6):698-707
references.pmid: 21658445
references.citation: Comp Med. 2003 Apr;53(2):140-6
references.pmid: 12784847
references.citation: Nat Rev Cancer. 2013 Sep;13(9):624-36
references.pmid: 23969693
references.citation: Mol Cell Endocrinol. 2011 Jan 30;332(1-2):58-66
references.pmid: 20932876
references.citation: Nat Rev Neurosci. 2008 Mar;9(3):195-205
references.pmid: 18270513
references.citation: Mol Cancer. 2009 Jun 25;8:40
references.pmid: 19555497
references.citation: J Neurobiol. 2003 Jan;54(1):283-311
references.pmid: 12486710
references.citation: BMC Genomics. 2012 Jul 20;13:323
references.pmid: 22817472
references.citation: Prog Neuropsychopharmacol Biol Psychiatry. 2011 Mar 30;35(2):561-7
references.pmid: 21187119
references.citation: Front Neural Circuits. 2013 May 27;7:99
references.pmid: 23750127
references.citation: Nat Neurosci. 2013 Aug;16(8):1154-61
references.pmid: 23792946
references.citation: Front Neural Circuits. 2013 May 06;7:82
references.pmid: 23653595
references.citation: Dev Dyn. 2011 Aug;240(8):1964-76
references.pmid: 21688347
references.citation: Prog Neuropsychopharmacol Biol Psychiatry. 2011 Aug 1;35(6):1409-15
references.pmid: 20837077
references.citation: Am J Med Genet B Neuropsychiatr Genet. 2013 Jun;162B(4):327-66
references.pmid: 23650212
references.citation: Trends Pharmacol Sci. 2014 Feb;35(2):63-75
references.pmid: 24412421
references.citation: Behav Brain Res. 2010 Apr 2;208(2):371-6
references.pmid: 20006651
references.citation: Trends Neurosci. 2002 Oct;25(10):506-9
references.pmid: 12220878
references.citation: Behav Brain Res. 2009 Dec 14;205(1):38-44
references.pmid: 19540270
references.citation: J Neurosci. 2010 Oct 13;30(41):13718-28
references.pmid: 20943912
references.citation: Biol Rev Camb Philos Soc. 2008 Feb;83(1):13-34
references.pmid: 18093234
references.citation: Neuroscience. 2013 Mar 13;233:54-63
references.pmid: 23270857
references.citation: Behav Brain Res. 2010 Dec 25;214(2):157-71
references.pmid: 20510300
references.citation: Physiol Behav. 2011 Oct 24;104(5):831-7
references.pmid: 21839758
references.citation: PLoS One. 2012;7(5):e36931
references.pmid: 22615849
references.citation: ILAR J. 2006;47(2):124-31
references.pmid: 16547369
references.citation: Am J Physiol Regul Integr Comp Physiol. 2008 Mar;294(3):R711-9
references.pmid: 18077507
references.citation: Curr Opin Neurobiol. 2013 Feb;23(1):119-26
references.pmid: 23246238
references.citation: Trends Cell Biol. 2013 Dec;23(12):584-6
references.pmid: 24275383
references.citation: Cell Stem Cell. 2008 Feb 7;2(2):183-9
references.pmid: 18371439
references.citation: Dev Neurobiol. 2012 Mar;72(3):386-94
references.pmid: 21567982
references.citation: Behav Brain Res. 2012 Jan 1;226(1):66-76
references.pmid: 21920389
references.citation: Neuropharmacology. 2012 Jan;62(1):135-43
references.pmid: 21843537
references.citation: Prog Neuropsychopharmacol Biol Psychiatry. 2008 Jul 1;32(5):1172-8
references.pmid: 18222590
references.citation: Nat Commun. 2013;4:2410
references.pmid: 24002024
references.citation: Behav Brain Res. 2010 Dec 25;214(2):277-84
references.pmid: 20561961
references.citation: Nat Med. 2009 Oct;15(10):1106-9
references.pmid: 19812559
references.citation: Nat Methods. 2013 May;10(5):413-20
references.pmid: 23524393
references.citation: J Neurosci. 2014 Jan 1;34(1):134-9
references.pmid: 24381274
references.citation: Prog Neuropsychopharmacol Biol Psychiatry. 2012 Apr 27;37(1):194-202
references.pmid: 22251567
references.citation: J Endocrinol. 2006 Jun;189(3):425-39
references.pmid: 16731775
references.citation: PLoS One. 2013 Oct 29;8(10):e77997
references.pmid: 24205060
references.citation: Behav Brain Res. 2012 Mar 17;228(2):333-8
references.pmid: 22192379
references.citation: Front Mol Neurosci. 2013 Mar 04;6:2
references.pmid: 23459413
references.citation: Neuron. 2014 Mar 19;81(6):1328-1343
references.pmid: 24656252
references.citation: Dev Neurobiol. 2012 Mar;72(3):395-403
references.pmid: 22328274
references.citation: J Pers Soc Psychol. 2000 Dec;79(6):1007-21
references.pmid: 11138751
references.citation: Prog Neuropsychopharmacol Biol Psychiatry. 2011 Aug 1;35(6):1432-51
references.pmid: 20971150
references.citation: Behav Brain Res. 2015 Jan 1;276:28-31
references.pmid: 24384129
references.citation: Curr Top Behav Neurosci. 2012;12:3-24
references.pmid: 22250005
references.citation: Zebrafish. 2012 Dec;9(4):191-4
references.pmid: 23244690
references.citation: PLoS One. 2013;8(1):e54515
references.pmid: 23349914
references.citation: ILAR J. 2012;53(2):114-25
references.pmid: 23382343
references.citation: Nat Protoc. 2010 Nov;5(11):1786-99
references.pmid: 21030954
references.citation: PLoS One. 2013 Oct 08;8(10):e75955
references.pmid: 24116082
references.citation: PLoS One. 2013;8(3):e51456
references.pmid: 23536755
references.citation: Science. 1999 Jun 4;284(5420):1670-2
references.pmid: 10356397
references.citation: Behav Brain Res. 2013 Jan 1;236(1):258-269
references.pmid: 22974549
references.citation: Prog Neuropsychopharmacol Biol Psychiatry. 2014 Apr 3;50:27-36
references.pmid: 24315837
references.citation: Acta Psychiatr Scand. 1986 Feb;73(2):113-27
references.pmid: 3486547
references.citation: PLoS One. 2013 Nov 01;8(11):e79406
references.pmid: 24223943
references.citation: J Endocrinol. 2013 Nov 06;219(3):251-8
references.pmid: 24198397
references.citation: Pharmacol Biochem Behav. 2013 Jul;108:16-27
references.pmid: 23603028
references.citation: Genes Brain Behav. 2013 Jul;12(5):576-82
references.pmid: 23679663
references.citation: Pharmacol Biochem Behav. 2013 May;106:137-42
references.pmid: 23558086
references.citation: Genes Brain Behav. 2002 Jan;1(1):3-8
references.pmid: 12886944
references.citation: Neurosci Biobehav Rev. 2010 Mar;34(3):373-86
references.pmid: 19698744
references.citation: Nat Methods. 2009 Dec;6(12):883-9
references.pmid: 19898484
references.citation: J Comp Neurol. 2001 Nov 26;440(4):342-77
references.pmid: 11745628
references.citation: Physiol Behav. 2008 Sep 3;95(1-2):36-47
references.pmid: 18499199
references.citation: Behav Pharmacol. 2013 Sep;24(5-6):375-83
references.pmid: 23811781
references.citation: Physiol Behav. 2001 Aug;73(5):705-17
references.pmid: 11566205
references.citation: Behav Brain Res. 2011 Jan 1;216(1):166-71
references.pmid: 20674614
references.citation: Neuroimage. 2010 May 15;51(1):76-82
references.pmid: 20139016
references.citation: Expert Opin Drug Discov. 2014 Jan;9(1):15-26
references.pmid: 24206163
references.citation: Physiol Behav. 2003 Aug;79(3):461-70
references.pmid: 12954440
references.citation: Zebrafish. 2008 Dec;5(4):247-55
references.pmid: 19133823
references.citation: Nat Rev Drug Discov. 2005 Sep;4(9):775-90
references.pmid: 16138108
references.citation: ACS Chem Neurosci. 2013 Aug 21;4(8):1137-50
references.pmid: 23883191
references.citation: Brain Res Bull. 2012 Feb 10;87(2-3):135-43
references.pmid: 22155548
references.citation: Neurotoxicol Teratol. 2011 Nov-Dec;33(6):658-67
references.pmid: 21683787
references.citation: Prog Neuropsychopharmacol Biol Psychiatry. 2011 Aug 1;35(6):1421-31
references.pmid: 21122812
references.citation: J Comp Neurol. 2012 Aug 1;520(11):2317-39, Spc1
references.pmid: 22581687
references.citation: PLoS One. 2013 Jul 29;8(7):e69661
references.pmid: 23922773
references.citation: Front Neural Circuits. 2009 Dec 11;3:21
references.pmid: 20126518
references.citation: Nat Neurosci. 2010 Nov;13(11):1354-6
references.pmid: 20935642
references.citation: Gerontologist. 1994 Oct;34(5):674-9
references.pmid: 7959136
references.citation: Neuron. 2008 Mar 27;57(6):809-18
references.pmid: 18367082
references.citation: PLoS One. 2011 Mar 07;6(3):e17597
references.pmid: 21408171
references.citation: Neuron. 2013 May 8;78(3):411-3
references.pmid: 23664609
references.citation: Front Syst Neurosci. 2013 Oct 10;7:65
references.pmid: 24133417
references.citation: Nat Methods. 2013 Jun;10(6):508-13
references.pmid: 23722210
references.citation: Dis Model Mech. 2011 Jul;4(4):433-8
references.pmid: 21708900
references.citation: Front Behav Neurosci. 2012 Oct 11;6:68
references.pmid: 23087630
references.citation: Behav Brain Res. 2008 Mar 17;188(1):168-77
references.pmid: 18054804
references.citation: Front Neural Circuits. 2013 Apr 09;7:65
references.pmid: 23576959
references.citation: Rev Neurosci. 2011;22(1):37-48
references.pmid: 21615260
references.citation: Behav Brain Res. 2012 Aug 1;233(2):450-7
references.pmid: 22677277
references.citation: Behav Brain Res. 2012 Apr 1;229(1):194-201
references.pmid: 22266470