title: Mast cell-restricted tetramer-forming tryptases and their beneficial roles in hemostasis and blood coagulation.
abstract: Tetramer-forming tryptase (hTryptase-β) was recently discovered to have a prominent role in preventing the internal accumulation of life-threatening fibrin deposits and fibrin-platelet clots. The anticoagulant activity of hTryptase-β is an explanation for the presence of hemorrhagic disorders in some patients with anaphylaxis or mastocytosis. The fragments of hFibrinogen formed by the proteolysis of this prominent protein by hTryptase-β could be used as biomarkers in the blood and/or urine for the identification and monitoring of patients with mast cell-dependent disorders. Recombinant hTryptase-β has potential to be used in clinical settings where it is desirable to inhibit blood coagulation.
authors.affiliation: Allergy Service, Hospital Universitario Gregorio Marañón, Instituto de Investigación Sanitaria Gregorio Marañón, Dr Esquerdo 46, Madrid 28007, Spain. Electronic address: aliprietog@gmail.com.
authors.forename: Alicia
authors.initials: A
authors.lastname: Prieto-García
authors.affiliation: Division of Rheumatology, Immunology, and Allergy, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, 1 Jimmy Fund Way, Smith Building, Boston, MA 02115, USA.
authors.forename: Mariana C
authors.initials: MC
authors.lastname: Castells
authors.affiliation: Priority Research Centre for Asthma and Respiratory Disease, Hunter Medical Research Institute and University of Newcastle, 1 Kookaburra Circuit, Newcastle, NSW 2300, Australia.
authors.forename: Philip M
authors.initials: PM
authors.lastname: Hansbro
authors.affiliation: Division of Rheumatology, Immunology, and Allergy, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, 1 Jimmy Fund Way, Smith Building, Boston, MA 02115, USA.
authors.forename: Richard L
authors.initials: RL
authors.lastname: Stevens
mesh_terms: D000707:Anaphylaxis / Q000097:blood / Q000235:genetics*; D001777:Blood Coagulation / Q000235:genetics*; D005337:Fibrin / Q000235:genetics / Q000378:metabolism; D005340:Fibrinogen / Q000235:genetics / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006632:Histamine / Q000378:metabolism; D006801:Humans; D007527:Isoenzymes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008407:Mast Cells / Q000201:enzymology* / Q000473:pathology; D008415:Mastocytosis / Q000097:blood / Q000235:genetics*; D055503:Protein Multimerization; D015398:Signal Transduction; D012867:Skin / Q000201:enzymology / Q000473:pathology; D013917:Thrombin / Q000235:genetics / Q000378:metabolism; D053802:Tryptases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: D007527:Isoenzymes; D006632:Histamine; D005337:Fibrin; D005340:Fibrinogen; D013917:Thrombin; C546348:TPSAB1 protein, human; D053802:Tryptases
keywords: Anaphylaxis; Fibrin thrombin-dependent coagulation; Fibrinogen; Mast cell; hTryptase-β; mMCP-6; mMCP-7