title: Determination of pinocembrin in human plasma by solid-phase extraction and LC/MS/MS: application to pharmacokinetic studies.
abstract: A sensitive, fast and specific method for the quantitation of pinocembrin in human plasma based on high-performance liquid chromatography-tandem mass spectrometry (LC/MS/MS) was developed and validated. Clonazepam was used as the internal standard (IS). After solid-phase extraction of 500 μL plasma, pinocembrin and the IS were separated on a Luna C8 column using the mobile phase composed of acetonitrile-0.3 mm ammonium acetate solution (65:35, v/v) at a flow rate of 0.25 mL/min in isocratic mode. The detection was performed on a triple quadrupole tandem mass spectrometer by multiple reaction monitoring via an electrospray ionization source in negative mode by AB SCIEX Qtrap 5500. The assay was linear from 1 to 400 ng/mL, with within- and between-run accuracy (relative error) from -1.82 to 0.54%, and within- and between-run precision (CV) below 5.25%. The recovery was above 88% for the analyte at 1, 50 and 300 ng/mL. This analytical method was successful for the determination of pinocembrin in human plasma and applied to a pharmacokinetic study of pinocembrin injection in healthy volunteers after intravenous drip administration.
authors.affiliation: Department of Clinical Pharmacology, Beijing Hospital, no. 1 Dahua Road, Beijing, 100730, China.
authors.forename: Bei
authors.initials: B
authors.lastname: Yan
authors.forename: Guoying
authors.initials: G
authors.lastname: Cao
authors.forename: Taohua
authors.initials: T
authors.lastname: Sun
authors.forename: Xi
authors.initials: X
authors.lastname: Zhao
authors.forename: Xin
authors.initials: X
authors.lastname: Hu
authors.forename: Jiling
authors.initials: J
authors.lastname: Yan
authors.forename: Yueying
authors.initials: Y
authors.lastname: Peng
authors.forename: Aixin
authors.initials: A
authors.lastname: Shi
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Xue
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.forename: Kexin
authors.initials: K
authors.lastname: Li
authors.forename: Yingfa
authors.initials: Y
authors.lastname: Liu
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D044950:Flavanones / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics*; D006801:Humans; D016014:Linear Models; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D044950:Flavanones; C016063:pinocembrin
keywords: LC/MS/MS; human plasma; method validation; pharmacokinetics; pinocembrin