title: Tetraspanin CD9 modulates human lymphoma cellular proliferation via histone deacetylase activity.
abstract: Non-Hodgkin Lymphoma (NHL) is a type of hematological malignancy that affects two percent of the overall population in the United States. Tetraspanin CD9 is a cell surface protein that has been thoroughly demonstrated to be a molecular facilitator of cellular phenotype. CD9 expression varies in two human lymphoma cell lines, Raji and BJAB. In this report, we investigated the functional relationship between CD9 and cell proliferation regulated by histone deacetylase (HDAC) activity in these two cell lines. Introduction of CD9 expression in Raji cells resulted in significantly increased cell proliferation and HDAC activity compared to Mock transfected Raji cells. The increase in CD9-Raji cell proliferation was significantly inhibited by HDAC inhibitor (HDACi) treatment. Pretreatment of BJAB cells with HDAC inhibitors resulted in a significant decrease in endogenous CD9 mRNA and cell surface expression. BJAB cells also displayed decreased cell proliferation after HDACi treatment. These results suggest a significant relationship between CD9 expression and cell proliferation in human lymphoma cells that may be modulated by HDAC activity.
authors.affiliation: Vascular Biology Center of Excellence, The University of Tennessee Health Science Center, Memphis, TN 38163, United States; Department of Medicine, The University of Tennessee Health Science Center, Memphis, TN 38163, United States; Department of Molecular Sciences, The University of Tennessee Health Science Center, Memphis, TN 38163, United States; Department of Surgery, The University of Tennessee Health Science Center, Memphis, TN 38163, United States.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Herr
authors.affiliation: Vascular Biology Center of Excellence, The University of Tennessee Health Science Center, Memphis, TN 38163, United States.
authors.forename: Celia M
authors.initials: CM
authors.lastname: Longhurst
authors.affiliation: Vascular Biology Center of Excellence, The University of Tennessee Health Science Center, Memphis, TN 38163, United States.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Baker
authors.affiliation: Department of Biology, Bioinformatics Program, University of Memphis, Memphis, TN 38152, United States.
authors.forename: Ramin
authors.initials: R
authors.lastname: Homayouni
authors.affiliation: Vascular Biology Center of Excellence, The University of Tennessee Health Science Center, Memphis, TN 38163, United States.
authors.forename: Henry E
authors.initials: HE
authors.lastname: Speich
authors.affiliation: Vascular Biology Center of Excellence, The University of Tennessee Health Science Center, Memphis, TN 38163, United States.
authors.forename: Jayaprakash
authors.initials: J
authors.lastname: Kotha
authors.affiliation: Vascular Biology Center of Excellence, The University of Tennessee Health Science Center, Memphis, TN 38163, United States; Department of Medicine, The University of Tennessee Health Science Center, Memphis, TN 38163, United States; Department of Molecular Sciences, The University of Tennessee Health Science Center, Memphis, TN 38163, United States; Department of Surgery, The University of Tennessee Health Science Center, Memphis, TN 38163, United States; Department of Biology, Bioinformatics Program, University of Memphis, Memphis, TN 38152, United States. Electronic address: ljennings@uthsc.edu.
authors.forename: Lisa K
authors.initials: LK
authors.lastname: Jennings
mesh_terms: D002051:Burkitt Lymphoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003474:Curcumin / Q000494:pharmacology; D044127:Epigenesis, Genetic; D015870:Gene Expression / Q000187:drug effects; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology; D006655:Histone Deacetylases / Q000378:metabolism*; D006801:Humans; D006877:Hydroxamic Acids / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D060245:Tetraspanin 29 / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
chemical_list: C554869:CD9 protein, human; D056572:Histone Deacetylase Inhibitors; D006877:Hydroxamic Acids; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D011994:Recombinant Proteins; D060245:Tetraspanin 29; C012589:trichostatin A; D006655:Histone Deacetylases; D003474:Curcumin
keywords: CD9; Cell proliferation; Histone deacetylase; Lymphoma; Tetraspanin