title: High-affinity glucose transport in Aspergillus nidulans is mediated by the products of two related but differentially expressed genes.
abstract: Independent systems of high and low affinity effect glucose uptake in the filamentous fungus Aspergillus nidulans. Low-affinity uptake is known to be mediated by the product of the mstE gene. In the current work two genes, mstA and mstC, have been identified that encode high-affinity glucose transporter proteins. These proteins' primary structures share over 90% similarity, indicating that the corresponding genes share a common origin. Whilst the function of the paralogous proteins is little changed, they differ notably in their patterns of expression. The mstC gene is expressed during the early phases of germination and is subject to CreA-mediated carbon catabolite repression whereas mstA is expressed as a culture tends toward carbon starvation. In addition, various pieces of genetic evidence strongly support allelism of mstC and the previously described locus sorA. Overall, our data define MstC/SorA as a high-affinity glucose transporter expressed in germinating conidia, and MstA as a high-affinity glucose transporter that operates in vegetative hyphae under conditions of carbon limitation.
authors.affiliation: Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos, Consejo Superior de Investigaciones Científicas (CSIC), Paterna, Valencia, Spain; The Gurdon Institute, University of Cambridge, Cambridge, United Kingdom.
authors.forename: Josep V
authors.initials: JV
authors.lastname: Forment
authors.affiliation: Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos, Consejo Superior de Investigaciones Científicas (CSIC), Paterna, Valencia, Spain; Institut de Génétique et Microbiologie, Université Paris-Sud, Orsay, France.
authors.forename: Michel
authors.initials: M
authors.lastname: Flipphi
authors.affiliation: Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos, Consejo Superior de Investigaciones Científicas (CSIC), Paterna, Valencia, Spain.
authors.forename: Luisa
authors.initials: L
authors.lastname: Ventura
authors.affiliation: Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos, Consejo Superior de Investigaciones Científicas (CSIC), Paterna, Valencia, Spain; Instituto de Ciencias de la Vid y del Vino (CSIC, Universidad de La Rioja, Gobierno de La Rioja), Logroño, Spain.
authors.forename: Ramón
authors.initials: R
authors.lastname: González
authors.affiliation: Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos, Consejo Superior de Investigaciones Científicas (CSIC), Paterna, Valencia, Spain; Biopolis SL, Parc Cientific Universitat de València, Paterna, Valencia, Spain.
authors.forename: Daniel
authors.initials: D
authors.lastname: Ramón
authors.affiliation: Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos, Consejo Superior de Investigaciones Científicas (CSIC), Paterna, Valencia, Spain.
authors.forename: Andrew P
authors.initials: AP
authors.lastname: Maccabe
mesh_terms: D001233:Aspergillus nidulans / Q000235:genetics* / Q000378:metabolism*; D001692:Biological Transport / Q000235:genetics; D015152:Blotting, Northern; D057465:Catabolite Repression / Q000235:genetics; D004734:Energy Metabolism / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D005838:Genotype; D005947:Glucose / Q000378:metabolism*; D051246:Glucose Transport Proteins, Facilitative / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D010802:Phylogeny; D013172:Spores, Fungal / Q000235:genetics; D013379:Substrate Specificity
chemical_list: D005656:Fungal Proteins; D051246:Glucose Transport Proteins, Facilitative; D005947:Glucose
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