title: Two-steps extraction of essential oil, polysaccharides and biphenyl cyclooctene lignans from Schisandra chinensis Baill fruits.
abstract: A method for two-steps extraction of essential oil, polysaccharides and lignans from Schisandra chinensis Baill had been established. Firstly, S. chinensis was extracted by hydro-distillation, the extracted solution was separated from the water-insoluble residue and precipitated by adding dehydrated alcohol after the essential oil was collected, and then the precipitate as polysaccharide was collected. Finally, second extraction was performed to obtained lignans from the water-insoluble residue with ultrasonic-microwave assisted extraction (UMAE) method. Response surface methodology was employed to optimize the UMAE parameters, the optimal conditions were as follows: microwave power 430W, ethanol concentration 84%, particle size of sample 120-mesh sieves, ratio of water to raw material 15 and extraction time 2.1min. Under these optimized conditions, the total extraction yields of five lignans (Schisandrol A, Schisantherin A, Deoxyschisandrin, Schisandrin B and Schisandrin C) had reached 14.22±0.135mg/g. Compared with the traditional method of direct extraction of different bioactive components in respective procedure, the extraction yields of polysaccharides and the five lignans had reached 99% and 95%, respectively. The mean recoveries of the 5 lignan compounds and polysaccharides were 97.75-101.08% and their RSD value was less than 3.88%.The approach proposed in this study not only improved the extraction yield of lignans, but also elevated the utilization of Schisandra resources.
authors.affiliation: College of Chemical & Pharmaceutical Engineering, Jilin Institute of Chemical Technology, Jilin 132022, China; School of Chemistry, Northeast Normal University, Changchun 130024, China.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Cheng
authors.affiliation: College of Chemical & Pharmaceutical Engineering, Jilin Institute of Chemical Technology, Jilin 132022, China. Electronic address: yanghjm@163.com.
authors.forename: Yingjie
authors.initials: Y
authors.lastname: Yang
authors.affiliation: College of Chemical & Pharmaceutical Engineering, Jilin Institute of Chemical Technology, Jilin 132022, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Chemical & Pharmaceutical Engineering, Jilin Institute of Chemical Technology, Jilin 132022, China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Liu
authors.affiliation: College of Chemical & Pharmaceutical Engineering, Jilin Institute of Chemical Technology, Jilin 132022, China.
authors.forename: Hongli
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Department of Chemistry, Longdong University, Qingyang 745000, China.
authors.forename: Haobin
authors.initials: H
authors.lastname: Hu
mesh_terms: D001713:Biphenyl Compounds / Q000032:analysis / Q000302:isolation & purification; D034242:Cyclooctanes / Q000032:analysis / Q000302:isolation & purification; D005638:Fruit; D017705:Lignans / Q000032:analysis / Q000302:isolation & purification*; D008872:Microwaves; D009822:Oils, Volatile / Q000032:analysis / Q000302:isolation & purification*; D010936:Plant Extracts / Q000737:chemistry; D011134:Polysaccharides / Q000032:analysis / Q000302:isolation & purification*; D032141:Schisandra / Q000737:chemistry*; D014465:Ultrasonics / Q000379:methods
chemical_list: D001713:Biphenyl Compounds; D034242:Cyclooctanes; D017705:Lignans; D009822:Oils, Volatile; D010936:Plant Extracts; D011134:Polysaccharides
keywords: Biphenyl cyclooctene lignans; Essential oil; Polysaccharides; Schisandra chinensis Baill; Ultrasonic/microwave assisted extraction