title: The association between gene polymorphisms and leukocytosis with thrombotic complications in patients with essential thrombocythemia and polycythemia vera.
abstract: OBJECTIVE
  Vascular events are a common complication in patients with polycythemia vera (PV) and essential thrombocythemia (ET). This study aimed to analyze the association between PAI-1 4G/5G and ACE I/D gene polymorphisms, and leukocytosis with thrombosis in patients with PV and ET.
  
  
  METHODS
  In total, 64 patients with ET and PV were evaluated. Arterial or venous thrombosis, such as cerebral transient ischemic attack, ischemic stroke, myocardial infarction, peripheral arterial thrombosis, deep venous thrombosis, and pulmonary embolism, were defined as a vascular event. DNA samples were screened for mutations via reverse hybridization strip assay.
  
  
  RESULTS
  In terms of PAI-1 gene polymorphism, the frequency of the 4G and 5G allele was 48.5% and 51.5%, respectively. The ACE allele frequency was 51.2% and 48.8% for D and I, respectively. There wasn't an association between occurrence of vascular events and the frequency of any allele. In terms of occurrence of vascular events, there weren't any significance differences between the patients that were carrying the ACE D/D homozygous allele to ACE I/D and those that carried the I/I allele (P = 0.93). There wasn't a significant difference in occurrence of vascular events between the PAI-1 5G/5G homozygote allele carriers, and the 4G/5G and 4G/4G allele carriers (P = 0.97). Vascular events were significantly more common in the patients with leukocytosis (leukocyte count >10 × 109 L-1) than in those without leukocytosis (leukocyte count ≤10 × 109 L-1) (P = 0.00). Age >60 years was also a significant risk factor for occurrence of vascular events(P = 0.008).
  
  
  CONCLUSIONS
  PAI-1 and ACE gene polymorphisms were not considered new risk factors for thrombosis in PV and ET patients. On the other hand, leukocytosis at diagnosis was associated with the occurrence of vascular events in the patients with ET and PV.
authors.affiliation: Kocaeli University, School of Medicine, Department of Hematology, Kocaeli, Turkey.
authors.forename: Ozgür
authors.initials: O
authors.lastname: Mehtap
authors.affiliation: Kocaeli University, School of Medicine, Department of Hematology, Kocaeli, Turkey.
authors.forename: Elif Birtaş
authors.initials: EB
authors.lastname: Ateşoğlu
authors.affiliation: Kocaeli University, School of Medicine, Department of Hematology, Kocaeli, Turkey.
authors.forename: Pınar
authors.initials: P
authors.lastname: Tarkun
authors.affiliation: Kocaeli University, School of Medicine, Department of Hematology, Kocaeli, Turkey.
authors.forename: Emel
authors.initials: E
authors.lastname: Gönüllü
authors.affiliation: Kocaeli University, School of Medicine, Department of Hematology, Kocaeli, Turkey.
authors.forename: Hakan
authors.initials: H
authors.lastname: Keski
authors.affiliation: Kocaeli University, School of Medicine, Department of Internal Medicine, Kocaeli, Turkey.
authors.forename: Yıldıray
authors.initials: Y
authors.lastname: Topçu
authors.affiliation: Kocaeli University, School of Medicine, Department of Medical Genetic, Kocaeli, Turkey.
authors.forename: Nilüfer
authors.initials: N
authors.lastname: Uzülmez
authors.affiliation: Kocaeli University, School of Medicine, Department of Medical Genetic, Kocaeli, Turkey.
authors.forename: Deniz
authors.initials: D
authors.lastname: Sünnetçi
authors.affiliation: Kocaeli University, School of Medicine, Department of Hematology, Kocaeli, Turkey.
authors.forename: Abdullah
authors.initials: A
authors.lastname: Hacıhanefioğlu
keywords: ACE I/D; Essential thrombocythemia; Leukocytosis; PAI-1 4G/5G; Polycythemia vera; Thrombosis
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