title: Clinical significance of nerve growth factor and tropomyosin-receptor-kinase signaling pathway in intrahepatic cholangiocarcinoma.
abstract: OBJECTIVE
  To investigate the correlation between nerve growth factor-tropomyosin-receptor-kinase (NGF-TrkA) signaling pathway and prognosis in intrahepatic cholangiocarcinoma (IHCC).
  
  
  METHODS
  NGF and TrkA expression in 83 samples of IHCC was assessed by immunohistochemistry. Correlations between NGF-TrkA expression and clinicopathological features were analyzed by χ² test. Moreover, we evaluated the association between NGF-TrkA and overall survival by univariate and multivariate analysis. With experiments in vitro, we investigated the crucial role of NGF-TrkA on proliferation and invasion of IHCC cells with recombinant NGF-β stimulation.
  
  
  RESULTS
  We found that NGF and TrkA expression was significantly related with differentiation (P = 0.024) and intraneural invasion (P = 0.003), respectively. Additionally, double higher expression of NGF and TrkA was identified as an independent prognostic factor in IHCC (P = 0.003). Moreover, we demonstrated that NGF-TrkA signaling pathway can promote IHCC proliferation and invasion.
  
  
  CONCLUSIONS
  NGF-TrkA double higher expression is an independent prognostic factor in IHCC. NGF-TrkA pathway can promote IHCC progression, indicating that NGF-TrkA may become a potential drug target.
authors.affiliation: Xiao-Qing Yang,Yun-Fei Xu, Sen Guo, Yi Liu, Shang-Lei Ning, Hui Yang, Yu-Xin Chen, Department of Hepatobiliary Surgery, Qilu Hospital of Shandong University, Jinan 250012, Shandong Province, China.
authors.forename: Xiao-Qing
authors.initials: XQ
authors.lastname: Yang
authors.affiliation: Xiao-Qing Yang,Yun-Fei Xu, Sen Guo, Yi Liu, Shang-Lei Ning, Hui Yang, Yu-Xin Chen, Department of Hepatobiliary Surgery, Qilu Hospital of Shandong University, Jinan 250012, Shandong Province, China.
authors.forename: Yun-Fei
authors.initials: YF
authors.lastname: Xu
authors.affiliation: Xiao-Qing Yang,Yun-Fei Xu, Sen Guo, Yi Liu, Shang-Lei Ning, Hui Yang, Yu-Xin Chen, Department of Hepatobiliary Surgery, Qilu Hospital of Shandong University, Jinan 250012, Shandong Province, China.
authors.forename: Sen
authors.initials: S
authors.lastname: Guo
authors.affiliation: Xiao-Qing Yang,Yun-Fei Xu, Sen Guo, Yi Liu, Shang-Lei Ning, Hui Yang, Yu-Xin Chen, Department of Hepatobiliary Surgery, Qilu Hospital of Shandong University, Jinan 250012, Shandong Province, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Xiao-Qing Yang,Yun-Fei Xu, Sen Guo, Yi Liu, Shang-Lei Ning, Hui Yang, Yu-Xin Chen, Department of Hepatobiliary Surgery, Qilu Hospital of Shandong University, Jinan 250012, Shandong Province, China.
authors.forename: Shang-Lei
authors.initials: SL
authors.lastname: Ning
authors.affiliation: Xiao-Qing Yang,Yun-Fei Xu, Sen Guo, Yi Liu, Shang-Lei Ning, Hui Yang, Yu-Xin Chen, Department of Hepatobiliary Surgery, Qilu Hospital of Shandong University, Jinan 250012, Shandong Province, China.
authors.forename: Xiao-Fei
authors.initials: XF
authors.lastname: Lu
authors.affiliation: Xiao-Qing Yang,Yun-Fei Xu, Sen Guo, Yi Liu, Shang-Lei Ning, Hui Yang, Yu-Xin Chen, Department of Hepatobiliary Surgery, Qilu Hospital of Shandong University, Jinan 250012, Shandong Province, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Yang
authors.affiliation: Xiao-Qing Yang,Yun-Fei Xu, Sen Guo, Yi Liu, Shang-Lei Ning, Hui Yang, Yu-Xin Chen, Department of Hepatobiliary Surgery, Qilu Hospital of Shandong University, Jinan 250012, Shandong Province, China.
authors.forename: Yu-Xin
authors.initials: YX
authors.lastname: Chen
mesh_terms: D000368:Aged; D001650:Bile Duct Neoplasms / Q000378:metabolism*; D001653:Bile Ducts, Intrahepatic / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D018281:Cholangiocarcinoma / Q000378:metabolism*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D020932:Nerve Growth Factor / Q000378:metabolism*; D011379:Prognosis; D020917:Receptor, trkA / Q000378:metabolism*; D015398:Signal Transduction; D016896:Treatment Outcome
chemical_list: C502240:NGF protein, human; D020932:Nerve Growth Factor; D020917:Receptor, trkA
keywords: Intrahepatic cholangiocarcinoma; Nerve growth factor; Prognosis; Progression; Tropomyosin-receptor-kinase
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