title: Biological effects of (125)i seeds radiation on A549 lung cancer cells: G2/M arrest and enhanced cell death.
abstract: External beam radiation (EBRT) and (125)I seeds continuous low dose rate radiation (CLDR) were used to treat patients with lung cancer. We herein investigated the biological effects of EBRT and CLDR on lung cancer cells. A549 human lung cancer cell line was thus exposed to different doses of EBRT and CLDR. CLDR was more efficient to inhibit cell growth than EBRT. CLDR induced increased DNA damage as evidenced by long-lasting p-H2AX activity. The enhanced inhibitory effects of CLDR on lung cancer cell growth may be, at least in part, due to the increased Bax/Bcl2 ratio and cyclin B1-mediated G2/M arrest.
authors.affiliation: Department of Radiation Oncology, Peking University Third Hospital, Beijing, P.R. China,1.
authors.forename: Ang
authors.initials: A
authors.lastname: Qu
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.forename: Jinna
authors.initials: J
authors.lastname: Li
authors.forename: Junjie
authors.initials: J
authors.lastname: Wang
authors.forename: Jingjia
authors.initials: J
authors.lastname: Liu
authors.forename: Yuzhu
authors.initials: Y
authors.lastname: Hou
authors.forename: Li
authors.initials: L
authors.lastname: Huang
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhao
mesh_terms: D059447:Cell Cycle Checkpoints / Q000235:genetics / Q000528:radiation effects*; D016923:Cell Death / Q000235:genetics / Q000528:radiation effects*; D045744:Cell Line, Tumor; D056744:Cyclin B1 / Q000096:biosynthesis; D004249:DNA Damage / Q000235:genetics / Q000528:radiation effects*; D059565:G2 Phase Cell Cycle Checkpoints / Q000235:genetics / Q000528:radiation effects; D015972:Gene Expression Regulation, Neoplastic / Q000528:radiation effects; D006801:Humans; D007457:Iodine Radioisotopes; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology / Q000532:radiotherapy; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis; D051028:bcl-2-Associated X Protein
chemical_list: D056744:Cyclin B1; D007457:Iodine Radioisotopes; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein
keywords: 125I continuous low dose rate radiation; DNA double-strand breaks; Lung cancer; Single external beam radiation