title: Acid-catalyzed hot-water extraction of docosahexaenoic acid (DHA)-rich lipids from Aurantiochytrium sp. KRS101.
abstract: In this study, acid-catalyzed hot-water extraction of docosahexaenoic acid (DHA)-rich lipids from Aurantiochytrium sp. was performed, and its yield-enhancing effects were investigated. The total fatty acid content of the Aurantiochytrium sp. was 482.5mg/g cell, of which 141.7mg/g cell (29.4% of total fatty acids) was DHA. The lipid-extraction yield by acid-catalyzed hot-water treatment was compared with those by organic solvents. Among the various acid-catalyzed hot-water treatment conditions, the most optimal were 1.00% H2SO4 concentration, 100°C, 30min, under which the lipid-extraction yield was 472.4mg/g cell, and most of the DHA was extracted (29.2% of total fatty acids). Acid-catalyzed hot-water extraction treatment markedly improved the lipid-extraction yield of Aurantiochytrium sp.
authors.affiliation: Biomass and Waste Energy Laboratory, Korea Institute of Energy Research, Daejeon 305-343, Republic of Korea; Department of Chemical & Biological Engineering, Korea University, Seoul 136-701, Republic of Korea.
authors.forename: Sun-A
authors.initials: SA
authors.lastname: Choi
authors.affiliation: Advanced Biomass R&D Center, KAIST, Daejeon 305-701, Republic of Korea.
authors.forename: Joo-Young
authors.initials: JY
authors.lastname: Jung
authors.affiliation: Department of Chemical and Biomolecular Engineering, KAIST, Daejeon 305-701, Republic of Korea.
authors.forename: Kyochan
authors.initials: K
authors.lastname: Kim
authors.affiliation: Biomass and Waste Energy Laboratory, Korea Institute of Energy Research, Daejeon 305-343, Republic of Korea.
authors.forename: Jin-Suk
authors.initials: JS
authors.lastname: Lee
authors.affiliation: Department of Chemical and Biomolecular Engineering, KAIST, Daejeon 305-701, Republic of Korea.
authors.forename: Jong-Hee
authors.initials: JH
authors.lastname: Kwon
authors.affiliation: Department of Chemical & Biological Engineering, Korea University, Seoul 136-701, Republic of Korea.
authors.forename: Seung Wook
authors.initials: SW
authors.lastname: Kim
authors.affiliation: Advanced Biomass R&D Center, KAIST, Daejeon 305-701, Republic of Korea; Department of Chemical and Biomolecular Engineering, KAIST, Daejeon 305-701, Republic of Korea.
authors.forename: Ji-Won
authors.initials: JW
authors.lastname: Yang
authors.affiliation: Biomass and Waste Energy Laboratory, Korea Institute of Energy Research, Daejeon 305-343, Republic of Korea. Electronic address: yearn@kier.re.kr.
authors.forename: Ji-Yeon
authors.initials: JY
authors.lastname: Park
mesh_terms: D004281:Docosahexaenoic Acids / Q000032:analysis*; D006358:Hot Temperature; D058086:Microalgae / Q000737:chemistry*; D058009:Stramenopiles / Q000737:chemistry*
chemical_list: D004281:Docosahexaenoic Acids
keywords: Acid-catalyzed hot-water extraction; Aurantiochytrium sp.; Docosahexaenoic acid; Fatty acid content; Microalgal lipids