title: Delimiting the origin of a B chromosome by FISH mapping, chromosome painting and DNA sequence analysis in Astyanax paranae (Teleostei, Characiformes).
abstract: Supernumerary (B) chromosomes have been shown to contain a wide variety of repetitive sequences. For this reason, fluorescent in situ hybridisation (FISH) is a useful tool for ascertaining the origin of these genomic elements, especially when combined with painting from microdissected B chromosomes. In order to investigate the origin of B chromosomes in the fish species Astyanax paranae, these two approaches were used along with PCR amplification of specific DNA sequences obtained from the B chromosomes and its comparison with those residing in the A chromosomes. Remarkably, chromosome painting with the one-arm metacentric B chromosome probe showed hybridization signals on entire B chromosome, while FISH mapping revealed the presence of H1 histone and 18S rDNA genes symmetrically placed in both arms of the B chromosome. These results support the hypothesis that the B chromosome of A. paranae is an isochromosome. Additionally, the chromosome pairs Nos. 2 or 23 are considered the possible B chromosome ancestors since both contain syntenic H1 and 18S rRNA sequences. The analysis of DNA sequence fragments of the histone and rRNA genes obtained from the microdissected B chromosomes showed high similarity with those obtained from 0B individuals, which supports the intraspecific origin of B chromosomes in A. paranae. Finally, the population hereby analysed showed a female-biased B chromosome presence suggesting that B chromosomes in this species could influence sex determinism.
authors.affiliation: Departamento de Morfologia, Instituto de Biociências, Universidade Estadual Paulista, Distrito de Rubião Junior, Botucatu, São Paulo, Brazil.
authors.forename: Duílio M Z de A
authors.initials: DM
authors.lastname: Silva
authors.affiliation: Departamento de Morfologia, Instituto de Biociências, Universidade Estadual Paulista, Distrito de Rubião Junior, Botucatu, São Paulo, Brazil.
authors.forename: José Carlos
authors.initials: JC
authors.lastname: Pansonato-Alves
authors.affiliation: Departamento de Morfologia, Instituto de Biociências, Universidade Estadual Paulista, Distrito de Rubião Junior, Botucatu, São Paulo, Brazil.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Utsunomia
authors.affiliation: Departamento de Morfologia, Instituto de Biociências, Universidade Estadual Paulista, Distrito de Rubião Junior, Botucatu, São Paulo, Brazil.
authors.forename: Cristian
authors.initials: C
authors.lastname: Araya-Jaime
authors.affiliation: Departamento de Genética, Universidad de Granada, Granada, Spain.
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Ruiz-Ruano
authors.affiliation: Departamento de Ciências Biológicas, Faculdade de Ciências, Universidade Estadual Paulista, Campus de Bauru, Bauru, São Paulo, Brazil.
authors.forename: Sandro Natal
authors.initials: SN
authors.lastname: Daniel
authors.affiliation: CAUNESP, Universidade Estadual Paulista, Campus Jaboticabal, Jaboticabal, São Paulo, Brazil.
authors.forename: Diogo Teruo
authors.initials: DT
authors.lastname: Hashimoto
authors.affiliation: Departamento de Morfologia, Instituto de Biociências, Universidade Estadual Paulista, Distrito de Rubião Junior, Botucatu, São Paulo, Brazil.
authors.forename: Cláudio
authors.initials: C
authors.lastname: Oliveira
authors.affiliation: Departamento de Genética, Universidad de Granada, Granada, Spain.
authors.forename: Juan Pedro M
authors.initials: JP
authors.lastname: Camacho
authors.affiliation: Departamento de Ciências Biológicas, Faculdade de Ciências, Universidade Estadual Paulista, Campus de Bauru, Bauru, São Paulo, Brazil.
authors.forename: Fábio
authors.initials: F
authors.lastname: Porto-Foresti
authors.affiliation: Departamento de Morfologia, Instituto de Biociências, Universidade Estadual Paulista, Distrito de Rubião Junior, Botucatu, São Paulo, Brazil.
authors.forename: Fausto
authors.initials: F
authors.lastname: Foresti
mesh_terms: D000818:Animals; D060125:Characiformes / Q000145:classification / Q000235:genetics*; D002871:Chromosome Banding; D002874:Chromosome Mapping; D020223:Chromosome Painting; D002875:Chromosomes; D004275:DNA, Ribosomal; D005260:Female; D014644:Genetic Variation; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA
chemical_list: D004275:DNA, Ribosomal
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