title: Effects of oxidative stress on red blood cell rheology in sickle cell patients.
abstract: Sickle cell anaemia (SS) and sickle cell-haemoglobin C disease (SC) patients exhibit severe red blood cell (RBC) rheological alterations involved in the development of several complications. The contribution of oxidative stress in these haemorheological abnormalities is still unknown. We compared RBC reactive oxygen species (ROS) and glutathione (GSH) content, and the haemorheological profile of SS (n = 11), SC (n = 11) and healthy subjects (n = 12) at baseline and after in-vitro treatment with t-butyl hydroperoxide (TBHP). We showed: (i) higher RBC ROS content in SS and SC patients, with the highest level observed in SS patients; (ii) lower RBC GSH content in sickle syndrome patients, especially in SS patients; (iii) TBHP increased RBC ROS production and decreased RBC GSH content in all groups; (iv) TBHP decreased RBC aggregation and increased the strength of RBC aggregates in all groups but the increase in RBC aggregates strength was greater in sickle cell patients; (v) TBHP decreased RBC deformability in the three groups but with a higher magnitude in sickle cell patients. These data suggest that RBCs from sickle cell patients have an exaggerated response to oxidative stress, which is accompanied by a profound abnormal haemorheological profile, with greater alterations in SS than in SC patients.
authors.affiliation: Inserm U 1134, Pointe-à-Pitre, Guadeloupe, France; Université des Antilles et de la Guyane, Pointe-à-Pitre, Guadeloupe, France; Laboratory of Excellence GR-Ex, Paris, France.
authors.forename: Régine
authors.initials: R
authors.lastname: Hierso
authors.forename: Xavier
authors.initials: X
authors.lastname: Waltz
authors.forename: Pierre
authors.initials: P
authors.lastname: Mora
authors.forename: Marc
authors.initials: M
authors.lastname: Romana
authors.forename: Nathalie
authors.initials: N
authors.lastname: Lemonne
authors.forename: Philippe
authors.initials: P
authors.lastname: Connes
authors.forename: Marie-Dominique
authors.initials: MD
authors.lastname: Hardy-Dessources
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000704:Analysis of Variance; D000755:Anemia, Sickle Cell / Q000097:blood*; D016022:Case-Control Studies; D004903:Erythrocyte Aggregation / Q000187:drug effects; D004913:Erythrocytes, Abnormal / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D005978:Glutathione / Q000378:metabolism; D006450:Hemoglobin SC Disease / Q000097:blood*; D018056:Hemorheology; D006801:Humans; D008875:Middle Aged; D016877:Oxidants / Q000494:pharmacology; D018384:Oxidative Stress / Q000502:physiology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D055815:Young Adult; D020122:tert-Butylhydroperoxide / Q000494:pharmacology
chemical_list: D016877:Oxidants; D017382:Reactive Oxygen Species; D020122:tert-Butylhydroperoxide; D005978:Glutathione
keywords: haemorheology; oxidative stress; sickle cell disease