title: Stimulation of luminescence of mycelium of luminous fungus Neonothopanus nambi by ionizing radiation.
abstract: The luminescent system of higher luminous fungi is not fully understood and the enzyme/substrate pair of the light emission reaction has not been isolated. It was suggested that luminescence of fungi involves oxidase-type enzymes, and reactive oxygen species are important for fungal light production. Generation of reactive oxygen species can be stimulated by ionizing irradiation, which has not been studied for luminous fungi. We report the effect of X-irradiation on the luminescence of fungus Neonothopanus nambi. Experiments were performed with mycelium on a home-built setup based on an X-ray tube and monochromator/photomultiplier tube. Application of X-rays does not change the emission spectrum, but after approximately 20 min of continuous irradiation, light production from unsupported mycelium starts growing and increases up to approximately five times. After peaking, its level decreases irrespective of the presence of X-irradiation. After staying at a certain level, light production collapses to zero, which is not related to the drying of the mycelium or thermal impact of radiation. The observed shape of kinetics is characteristic of a multistage and/or chain reaction. The time profile of light production must reflect the current levels of radicals present in the system and/or the activity of enzyme complexes involved in light production.
authors.affiliation: Institute of Chemical Kinetics and Combustion SB RAS, 630090, Novosibirsk, Russia.
authors.forename: Tatiana V
authors.initials: TV
authors.lastname: Kobzeva
authors.forename: Anatoly R
authors.initials: AR
authors.lastname: Melnikov
authors.forename: Tatiana Y
authors.initials: TY
authors.lastname: Karogodina
authors.forename: Samat B
authors.initials: SB
authors.lastname: Zikirin
authors.forename: Dmitri V
authors.initials: DV
authors.lastname: Stass
authors.forename: Yuri N
authors.initials: YN
authors.lastname: Molin
authors.forename: Emma K
authors.initials: EK
authors.lastname: Rodicheva
authors.forename: Svetlana E
authors.initials: SE
authors.lastname: Medvedeva
authors.forename: Alexey P
authors.initials: AP
authors.lastname: Puzyr
authors.forename: Andrey A
authors.initials: AA
authors.lastname: Burov
authors.forename: Vladimir S
authors.initials: VS
authors.lastname: Bondar
authors.forename: Joseph I
authors.initials: JI
authors.lastname: Gitelson
mesh_terms: D000363:Agaricales / Q000737:chemistry* / Q000378:metabolism; D049449:Luminescence; D025282:Mycelium / Q000737:chemistry* / Q000378:metabolism; D011839:Radiation, Ionizing; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species
keywords: Higher luminous fungi; Neonothopanus nambi; ionizing irradiation; lipid peroxidation; reactive oxygen species