title: ACTIN-RELATED PROTEIN6 Regulates Female Meiosis by Modulating Meiotic Gene Expression in Arabidopsis.
abstract: In flowering plants, meiocytes develop from subepidermal cells in anthers and ovules. The mechanisms that integrate gene-regulatory processes with meiotic programs during reproductive development remain poorly characterized. Here, we show that Arabidopsis thaliana plants deficient in ACTIN-RELATED PROTEIN6 (ARP6), a subunit of the SWR1 ATP-dependent chromatin-remodeling complex, exhibit defects in prophase I of female meiosis. We found that this meiotic defect is likely due to dysregulated expression of meiotic genes, particularly those involved in meiotic recombination, including DMC1 (DISRUPTED MEIOTIC cDNA1). Analysis of DMC1 expression in arp6 mutant plants indicated that ARP6 inhibits expression of DMC1 in the megasporocyte and surrounding nonsporogeneous ovule cells before meiosis. After cells enter meiosis, however, ARP6 activates DMC1 expression specifically in the megasporocyte even as it continues to inhibit DMC1 expression in the nonsporogenous ovule cells. We further show that deposition of the histone variant H2A.Z, mediated by the SWR1 chromatin-remodeling complex at the DMC1 gene body, requires ARP6. Therefore, ARP6 regulates female meiosis by determining the spatial and temporal patterns of gene expression required for proper meiosis during ovule development.
authors.affiliation: National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China School of Plant Sciences, University of Arizona, Tucson, Arizona 85721.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Qin
authors.affiliation: National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Zhao
authors.affiliation: School of Plant Sciences, University of Arizona, Tucson, Arizona 85721.
authors.forename: Megan I
authors.initials: MI
authors.lastname: Skaggs
authors.affiliation: Center for Cellular and Molecular Biology, Hyderabad 500007, India.
authors.forename: Sebastien
authors.initials: S
authors.lastname: Andreuzza
authors.affiliation: School of Plant Sciences, University of Arizona, Tucson, Arizona 85721.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Tsukamoto
authors.affiliation: Center for Cellular and Molecular Biology, Hyderabad 500007, India.
authors.forename: Aneesh
authors.initials: A
authors.lastname: Panoli
authors.affiliation: School of Plant Sciences, University of Arizona, Tucson, Arizona 85721.
authors.forename: Kirsten N
authors.initials: KN
authors.lastname: Wallace
authors.affiliation: School of Natural Resources and the Environment, University of Arizona, Tucson, Arizona 85721.
authors.forename: Steven
authors.initials: S
authors.lastname: Smith
authors.affiliation: Center for Cellular and Molecular Biology, Hyderabad 500007, India.
authors.forename: Imran
authors.initials: I
authors.lastname: Siddiqi
authors.affiliation: Center for Plant Cell Biology and Department of Botany and Plant Sciences, University of California, Riverside, California 92521.
authors.forename: Zhenbiao
authors.initials: Z
authors.lastname: Yang
authors.affiliation: School of Plant Sciences, University of Arizona, Tucson, Arizona 85721.
authors.forename: Ramin
authors.identifier: http://orcid.org/0000-0002-0975-2984
authors.initials: R
authors.lastname: Yadegari
authors.affiliation: School of Plant Sciences, University of Arizona, Tucson, Arizona 85721 rpalaniv@email.arizona.edu.
authors.forename: Ravishankar
authors.identifier: http://orcid.org/0000-0003-0564-5564
authors.initials: R
authors.lastname: Palanivelu
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