title: Identification of absorbed constituents in the rabbit plasma and cerebrospinal fluid after intranasal administration of Asari Radix et Rhizoma by HS-SPME-GC-MS and HPLC-APCI-IT-TOF-MSn.
abstract: Traditional Chinese Medicine (TCM) nasal therapy has been utilized to treat numerous diseases for over two millennia. It has many advantages compared with other routes. In this article, headspace-solid phase microextraction-gas chromatography-mass spectrometry and high performance liquid chromatography-atmospheric pressure chemical ionization-ion trap-time of flight-multistage mass spectrometry were applied for the first time to analyze the absorbed constituents in rabbit plasma and cerebrospinal fluid (CSF) after intranasal administration of Asari Radix et Rhizoma (AR). In total, 47 absorbed AR constituents including 14 monoterpenes, 10 phenylpropanoids, four benzene derivatives, two alkanes, nine N-alkylamides and eight lignans were tentatively identified in the rabbit plasma and CSF. Thirty-three absorbed constituents are found to have different bioactivities related to the pharmacological actions of AR through bibliography data retrieval. These indicated that many types of constituents of TCM can be absorbed at the nasal cavity into both rabbit blood and CSF. This is the first study to explore the absorption of AR, and comprehensively analyze the absorbed constituents after intranasal administration of TCM. These findings extend our understanding of the effective substances of AR, and inspire us to make a hypothesis on the mechanism of additive effect of multiple constituents of TCMs, which is very worthy of further investigation.
authors.affiliation: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University Health Science Center, No.38, Xueyuan Road, Beijing 100191, China. clearfont@163.com.
authors.forename: Chen
authors.initials: C
authors.lastname: Li
authors.affiliation: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University Health Science Center, No.38, Xueyuan Road, Beijing 100191, China. xufeng_pharm@163.com.
authors.forename: Feng
authors.initials: F
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University Health Science Center, No.38, Xueyuan Road, Beijing 100191, China. xlzh99@163.com.
authors.forename: De-Mei
authors.initials: DM
authors.lastname: Xie
authors.affiliation: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University Health Science Center, No.38, Xueyuan Road, Beijing 100191, China. jing_jy@126.com.
authors.forename: Yu
authors.initials: Y
authors.lastname: Jing
authors.affiliation: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University Health Science Center, No.38, Xueyuan Road, Beijing 100191, China. myshang@bjmu.edu.cn.
authors.forename: Ming-Ying
authors.initials: MY
authors.lastname: Shang
authors.affiliation: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University Health Science Center, No.38, Xueyuan Road, Beijing 100191, China. guangxl@bjmu.edu.cn.
authors.forename: Guang-Xue
authors.initials: GX
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University Health Science Center, No.38, Xueyuan Road, Beijing 100191, China. xuanwang6818@bjmu.edu.cn.
authors.forename: Xuan
authors.initials: X
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University Health Science Center, No.38, Xueyuan Road, Beijing 100191, China. sqcai@bjmu.edu.cn.
authors.forename: Shao-Qing
authors.initials: SQ
authors.lastname: Cai
mesh_terms: D000281:Administration, Intranasal; D000473:Alkanes / Q000097:blood / Q000134:cerebrospinal fluid; D000818:Animals; D001555:Benzene Derivatives / Q000097:blood / Q000134:cerebrospinal fluid; D004365:Drugs, Chinese Herbal; D017705:Lignans / Q000097:blood / Q000134:cerebrospinal fluid; D029562:Magnoliaceae / Q000737:chemistry*; D008297:Male; D039821:Monoterpenes / Q000097:blood / Q000134:cerebrospinal fluid; D010936:Plant Extracts / Q000097:blood / Q000134:cerebrospinal fluid / Q000302:isolation & purification / Q000493:pharmacokinetics*; D053284:Polyunsaturated Alkamides / Q000097:blood / Q000134:cerebrospinal fluid; D011422:Propionates / Q000097:blood / Q000134:cerebrospinal fluid; D011817:Rabbits; D027343:Rhizome / Q000737:chemistry*
chemical_list: D000473:Alkanes; D001555:Benzene Derivatives; D004365:Drugs, Chinese Herbal; D017705:Lignans; D039821:Monoterpenes; D010936:Plant Extracts; D053284:Polyunsaturated Alkamides; D011422:Propionates
references.citation: J Mass Spectrom. 2008 Jan;43(1):97-107
references.pmid: 17729382
references.citation: J Pharm Biomed Anal. 2013 Nov;85:231-8
references.pmid: 23973758
references.citation: Chem Biodivers. 2008 Feb;5(2):346-51
references.pmid: 18293448
references.citation: Rapid Commun Mass Spectrom. 2007;21(22):3613-20
references.pmid: 17939160
references.citation: J Chromatogr A. 2006 Jan 27;1103(2):219-28
references.pmid: 16298376
references.citation: J Agric Food Chem. 2012 Dec 12;60(49):12131-41
references.pmid: 23181941
references.citation: J Control Release. 2003 Feb 21;87(1-3):187-98
references.pmid: 12618035
references.citation: J Agric Food Chem. 2011 Aug 10;59(15):8086-94
references.pmid: 21702479
references.citation: Bioorg Med Chem Lett. 2012 Apr 1;22(7):2527-33
references.pmid: 22381047
references.citation: Zhongguo Zhong Yao Za Zhi. 2010 Jul;35(14):1871-3
references.pmid: 20939288
references.citation: Rapid Commun Mass Spectrom. 2008 Nov;22(22):3615-23
references.pmid: 18946863
references.citation: J Pharm Biomed Anal. 2010 Nov 2;53(3):243-9
references.pmid: 20227845
references.citation: Int J Pharm. 2009 Jun 22;375(1-2):55-60
references.pmid: 19481691
references.citation: J Pharm Pharm Sci. 2009;12(3):288-311
references.pmid: 20067706