title: Alginate-based composite sponge containing silver nanoparticles synthesized in situ.
abstract: Silver-based biomaterials have been developed in a variety of bactericidal applications, especially for wound dressings. In this study, silver nanoparticles (AgNPs) were synthesized in a sodium alginate solution and then the composite sponge containing AgNPs was prepared from the nanocolloid solution. The alginate-stabilized AgNPs had the mean negative zeta potential of -52.5mV, suggesting that the surface charge prevents the nanoparticles from aggregating through electrostatic repulsion. The alginate-AgNPs composite sponge had a highly enhanced antimicrobial activity compared to the alginate sponge. In spite of excellent cytocompatibility of the alginate sponge, the viability of the cell treated with the alginate-AgNPs composite sponge extract decreased to 86% of the control. The amount of proinflammatory cytokines released from macrophages treated with the alginate-AgNPs composite sponge was reduced. For the preparation of AgNPs-embedded composites, alginate can be a potential candidate stabilizing AgNPs and providing synergistic antimicrobial and antiinflammatory activities with AgNPs.
authors.affiliation: Division of Nano & Bio Technology, Daegu Gyungbuk Institute of Science & Technology, Daegu 711-873, Republic of Korea.
authors.forename: Song Yi
authors.initials: SY
authors.lastname: Seo
authors.affiliation: Division of Nano & Bio Technology, Daegu Gyungbuk Institute of Science & Technology, Daegu 711-873, Republic of Korea.
authors.forename: Ga Hyun
authors.initials: GH
authors.lastname: Lee
authors.affiliation: Division of Nano & Bio Technology, Daegu Gyungbuk Institute of Science & Technology, Daegu 711-873, Republic of Korea.
authors.forename: Se Guen
authors.initials: SG
authors.lastname: Lee
authors.affiliation: Department of Advanced Organic Materials Science and Engineering, Kyungpook National University, Daegu 702-701, Republic of Korea.
authors.forename: So Yeon
authors.initials: SY
authors.lastname: Jung
authors.affiliation: Joint Institute for Regenerative Medicine, Kyungpook National University Hospital, Daegu 700-721, Republic of Korea; Biomedical Research Institute, School of Medicine, Kyungpook National University Hospital, Daegu 700-721, Republic of Korea.
authors.forename: Jeong Ok
authors.initials: JO
authors.lastname: Lim
authors.affiliation: Department of Advanced Organic Materials Science and Engineering, Kyungpook National University, Daegu 702-701, Republic of Korea; Department of Bio-fibers and materials Science, Kyungpook National University, Daegu 702-701, Republic of Korea.
authors.forename: Jin Hyun
authors.initials: JH
authors.lastname: Choi
mesh_terms: D000464:Alginates / Q000737:chemistry* / Q000494:pharmacology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D000893:Anti-Inflammatory Agents / Q000737:chemistry* / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D001424:Bacterial Infections / Q000188:drug therapy; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D002460:Cell Line; D020723:Glucuronic Acid / Q000737:chemistry / Q000494:pharmacology; D006603:Hexuronic Acids / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D053768:Metal Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D051379:Mice; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D012834:Silver / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D000464:Alginates; D000900:Anti-Bacterial Agents; D000893:Anti-Inflammatory Agents; D001672:Biocompatible Materials; D006603:Hexuronic Acids; D012834:Silver; D020723:Glucuronic Acid