title: Production and purification of a fungal chitosanase and chitooligomers from Penicillium janthinellum D4 and discovery of the enzyme activators.
abstract: Chitosanases have received much attention because of their wide range of applications. Although most fungal chitosanases use sugar as their major carbon source, in the present work, a chitosanase was induced from a squid pen powder (SPP)-containing Penicillium janthinellum D4 medium and purified by ammonium sulphate precipitation and combined column chromatography. The purified D4 chitosanase exhibited optimum activity at pH 7-9, 60°C and was stable at pH 7-11, 25-50°C. The D4 chitosanase that was used for chitooligomers preparation was studied. The enzyme products revealed various chitooligomers with different degrees of polymerisation (DP) from 3 to 9, as determined by a MALDI-TOF mass spectrometer, confirming the endo-type nature of the D4 chitosanase. D4 chitosanase activity was significantly inhibited by Cu(2+), Mn(2+), and EDTA. However, Fe(2+) activated or inhibited D4 chitosanases at different concentrations. The D4 chitosanase was also activated by some small synthetic boron-containing molecules with boronate ester side chains.
authors.affiliation: Biotechnology Centre, Tay Nguyen University, 567 Le Duan Street, Buon Ma Thuot, Viet Nam.
authors.forename: Anh Dzung
authors.initials: AD
authors.lastname: Nguyen
authors.affiliation: Department of Chemistry, Tamkang University, New Taipei City 25137, Taiwan.
authors.forename: Chun-Ching
authors.initials: CC
authors.lastname: Huang
authors.affiliation: Department of Chemistry, Tamkang University, New Taipei City 25137, Taiwan; Life Science Development Center, Tamkang University, New Taipei City 25137, Taiwan.
authors.forename: Tzu-Wen
authors.initials: TW
authors.lastname: Liang
authors.affiliation: Experimental Biology Department, Faculty of Natural Science and Technology, Tay Nguyen University, 567 Le Duan Street, Buon Ma Thuot, Viet Nam.
authors.forename: Van Bon
authors.initials: VB
authors.lastname: Nguyen
authors.affiliation: Department of Chemistry, Tamkang University, New Taipei City 25137, Taiwan.
authors.forename: Po-Shen
authors.initials: PS
authors.lastname: Pan
authors.affiliation: Department of Chemistry, Tamkang University, New Taipei City 25137, Taiwan; Life Science Development Center, Tamkang University, New Taipei City 25137, Taiwan. Electronic address: sabulo@mail.tku.edu.tw.
authors.forename: San-Lang
authors.initials: SL
authors.lastname: Wang
mesh_terms: D001895:Boron / Q000737:chemistry; D002686:Chitin / Q000737:chemistry; D006026:Glycoside Hydrolases / Q000378:metabolism*; D010407:Penicillium / Q000737:chemistry*
chemical_list: D002686:Chitin; D006026:Glycoside Hydrolases; C019868:chitosanase; D001895:Boron
keywords: Boron; Chitooligomers; Chitosanase; Chitosanase activator; Penicillium janthinellum; Squid pen