title: Role of platelet derived growth factor (PDGF) in reverting neuronal nuclear and soma size alterations in NSC-34 cells exposed to cerebrospinal fluid from amyotrophic lateral sclerosis patients.
abstract: OBJECTIVE
  Amyotrophic lateral sclerosis (ALS) or motor neuron disease is an adult-onset progressive neurodegenerative disorder. ALS-CSF has been shown to produce toxic effects on motor neuron cells like aberrant neurofilament phosphorylation and morphological alterations of nuclear and soma size. Our current study was designed to investigate the neuroprotective role of platelet derived growth factor (PDGF) in reverting the adverse effects produced by ALS-CSF.
  
  
  METHODS
  Our present study was carried out to determine the restorative potential of PDGF on the toxic effects of ALS-CSF on NSC motor neuron cells from patients. Therefore the cells were divided in to three groups: (a) normal control (NC) - the cells were not exposed to ALS-CSF; (b) ALS group - the cells were exposed to ALS-CSF; (c) NALS group - the cells were exposed to non ALS CSF. Further each of these groups was supplemented with PDGF.
  
  
  CONCLUSIONS
  We observed that the mean area of nucleus and cell soma of the differentiated NSC motor neuron cells was significantly reduced in the cells exposed to ALS-CSF. We also observed that subsequent treatment with PDGF restored the soma area and nucleus of the ALS-CSF exposed cells significantly. Taken together, we show that supplementation with PDGF restores the morphological changes induced by ALS-CSF and PDGF may play a significant role in protecting motor neurons from apoptosis in ALS and thereby it promoting the cell survival.
authors.affiliation: Department of Orthopedics, General Hospital of Guangzhou Military Area Command of Chinese PLA, Guangzhou 5000, Guangdong Province, China.
authors.forename: Yu-Yue
authors.initials: YY
authors.lastname: Chen
authors.affiliation: Department of Orthopedics, General Hospital of Shenyang Military Area Command of Chinese PLA, Rescue Center of Severe Wound and Trauma of Chinese PLA, Shenyang 110840, Liaoning Province, China.
authors.forename: Xin-Wei
authors.initials: XW
authors.lastname: Liu
authors.affiliation: Department of Materials Engineering, University of British Columbia, Vancouver, British Columia, V6T1Z4, Canada.
authors.forename: Tian-Xing
authors.initials: TX
authors.lastname: Gong
authors.affiliation: Department of Orthopedics, The Forth Affiliated Hospital of China Medical University, Shenyang 11003, Liaoning Province, China.
authors.forename: Zhi-Yu
authors.initials: ZY
authors.lastname: Zhang
authors.affiliation: Laboratory of severe and war-related trauma center, General Hospital of Shenyang Military Area Command of Chinese PLA, Shenyang 110840, Liaoning Province, China.
authors.forename: Yun-En
authors.initials: YE
authors.lastname: Liu
authors.affiliation: Laboratory of severe and war-related trauma center, General Hospital of Shenyang Military Area Command of Chinese PLA, Shenyang 110840, Liaoning Province, China.
authors.forename: Yu-Biao
authors.initials: YB
authors.lastname: Zhang
authors.affiliation: Department of Orthopedics, General Hospital of Shenyang Military Area Command of Chinese PLA, Rescue Center of Severe Wound and Trauma of Chinese PLA, Shenyang 110840, Liaoning Province, China.
authors.forename: Liang-Bi
authors.initials: LB
authors.lastname: Xiang
authors.affiliation: Department of Orthopedics, General Hospital of Guangzhou Military Area Command of Chinese PLA, Guangzhou 5000, Guangdong Province, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Xia
mesh_terms: D000328:Adult; D000690:Amyotrophic Lateral Sclerosis / Q000134:cerebrospinal fluid*; D002460:Cell Line; D002555:Cerebrospinal Fluid; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009046:Motor Neurons / Q000187:drug effects* / Q000473:pathology; D018696:Neuroprotective Agents / Q000494:pharmacology*; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology* / Q000502:physiology
chemical_list: D018696:Neuroprotective Agents; D010982:Platelet-Derived Growth Factor
keywords: Cell death; Motor neuron disease; Neurodegeneration; PDGF