title: mRNA and microRNA purity and integrity: the key to success in expression profiling.
abstract: RNA quality control is a crucial step in guaranteeing integer nondegraded RNA and receiving meaningful results in gene expression profiling experiments, using micro-array, RT-qPCR (Reverse-Transcription quantitative PCR), or Next-Generation-Sequencing by RNA-Seq or small-RNA Seq. Therefore, assessment of RNA integrity and purity is very essential prior to gene expression analysis of sample RNA to ensure the accuracy of any downstream applications. RNA samples should be nondegraded or fragmented and free of protein, genomic DNA, nucleases, and enzymatic inhibitors. Herein we describe the current state-of-the-art RNA quality assessment by combining UV/Vis spectrophotometry and microfluidic capillary electrophoresis.
authors.affiliation: Physiology Weihenstephan, ZIEL Research Center for Nutrition and Food Sciences Technische Universität München, Weihenstephaner Berg 3, D-85354, Freising, Germany.
authors.forename: Benedikt
authors.initials: B
authors.lastname: Kirchner
authors.forename: Vijay
authors.initials: V
authors.lastname: Paul
authors.forename: Irmgard
authors.initials: I
authors.lastname: Riedmaier
authors.forename: Michael W
authors.initials: MW
authors.lastname: Pfaffl
mesh_terms: D000818:Animals; D002417:Cattle; D019075:Electrophoresis, Capillary; D020869:Gene Expression Profiling / Q000379:methods*; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D046210:Microfluidic Analytical Techniques; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics*
chemical_list: D035683:MicroRNAs; D012333:RNA, Messenger