title: Gankyrin is frequently overexpressed in cervical high grade disease and is associated with cervical carcinogenesis and metastasis.
abstract: Our previous studies have showed that Gankyrin expression is correlated with a malignant phenotype in endometrial carcinoma. Here, we investigated the possible role of Gankyrin in cervical disease. The increasing protein level of Gankyrin was observed in high-grade cervical intraepithelial neoplasia and carcinoma compared with benign cervical tissues and low-grade cervical intraepithelial neoplasia. In para-carcinoma tissues, it was found interestingly that there was no lymph node metastasis when nuclei Gankyrin was positively expressed, but lymph node metastasis rate was 30% (6/20) when nuclei Gankyrin was negatively expressed. In vitro, the transfection of Gankyrin resulted in markedly up-regulating of Vimentin, β-catenin and Twist2, as well as down-regulating of E-cadherin in cervical carcinoma cells. Our results suggested that Gankyrin may be functional in cervical carcinogenesis and metastasis.
authors.affiliation: Department of Obstetrics and Gynecology, Shanghai First People's Hospital, Shanghai Jiaotong University, Shanghai, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Obstetrics and Gynecology, Shanghai Tenth People's Hospital, Shanghai Tongji University, Shanghai, China.
authors.forename: Jiawen
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Gynecology and Obstetrics, Kunshan Hospital of Traditional Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Jiangsu, China.
authors.forename: Wenyan
authors.initials: W
authors.lastname: Qian
authors.affiliation: Department of Obstetrics and Gynecology, Shanghai First People's Hospital, Shanghai Jiaotong University, Shanghai, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Dong
authors.affiliation: Department of Obstetrics and Gynecology, Shanghai First People's Hospital, Shanghai Jiaotong University, Shanghai, China.
authors.forename: Yongbin
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Division of Cancer Medicine, Department of Lymphoma and Myeloma, Center for Cancer Immunology Research, The University of Texas M. D. Anderson Cancer Center, Houston, Texas, United States of America.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Obstetrics and Gynecology, Shanghai First People's Hospital, Shanghai Jiaotong University, Shanghai, China.
authors.forename: Youji
authors.initials: Y
authors.lastname: Feng
authors.affiliation: Cancer Biology Medical Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Ding
authors.initials: D
authors.lastname: Ma
authors.affiliation: Department of Obstetrics and Gynecology, Shanghai First People's Hospital, Shanghai Jiaotong University, Shanghai, China.
authors.forename: Zhenbo
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Obstetrics and Gynecology, Shanghai First People's Hospital, Shanghai Jiaotong University, Shanghai, China.
authors.forename: Sufang
authors.initials: S
authors.lastname: Wu
mesh_terms: D063646:Carcinogenesis / Q000378:metabolism* / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002584:Cervix Uteri / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008207:Lymphatic Metastasis / Q000473:pathology*; D008875:Middle Aged; D060787:Neoplasm Grading; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000378:metabolism; D010957:Plasmids / Q000378:metabolism; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000378:metabolism; D002583:Uterine Cervical Neoplasms / Q000378:metabolism* / Q000473:pathology*
chemical_list: D009363:Neoplasm Proteins; C403591:PSMD10 protein, human; D011518:Proto-Oncogene Proteins; D034741:RNA, Small Interfering; D046988:Proteasome Endopeptidase Complex
references.citation: Trends Cell Biol. 2006 May;16(5):229-33
references.pmid: 16581249
references.citation: Gynecol Oncol. 2012 Jan;124(1):112-8
references.pmid: 22018873
references.citation: Arch Gynecol Obstet. 2013 Mar;287(3):541-8
references.pmid: 23263171
references.citation: Int J Cancer. 2008 Jan 15;122(2):325-32
references.pmid: 17935131
references.citation: CA Cancer J Clin. 2012 Jan-Feb;62(1):10-29
references.pmid: 22237781
references.citation: Am J Transl Res. 2012;4(1):1-13
references.pmid: 22347518
references.citation: Cancer Lett. 2013 Oct 10;339(2):279-87
references.pmid: 23142288
references.citation: Cancer Lett. 2012 Jun 1;319(1):89-97
references.pmid: 22222214
references.citation: PLoS One. 2013 Nov 11;8(11):e78200
references.pmid: 24244294
references.citation: Int J Gynecol Cancer. 2013 Jun;23(5):906-15
references.pmid: 23640294
references.citation: Hepatology. 2014 Mar;59(3):935-46
references.pmid: 24037855
references.citation: Cancer Lett. 2010 Nov 1;297(1):9-17
references.pmid: 20483533
references.citation: Cancer Res. 2013 Jul 15;73(14):4548-58
references.pmid: 23576566
references.citation: Oncol Rep. 2012 Feb;27(2):504-10
references.pmid: 22075757
references.citation: World J Gastroenterol. 2002 Aug;8(4):638-43
references.pmid: 12174370
references.citation: Exp Mol Pathol. 2013 Apr;94(2):360-5
references.pmid: 23276718
references.citation: Ann N Y Acad Sci. 2002 Jun;963:21-36
references.pmid: 12095925
references.citation: BMC Cancer. 2010 Aug 17;10:437
references.pmid: 20716363
references.citation: Nat Rev Cancer. 2007 Jan;7(1):11-22
references.pmid: 17186016
references.citation: Hepatology. 2011 Jan;53(1):181-92
references.pmid: 21254169
references.citation: Oncogene. 2013 Jul 18;32(29):3452-60
references.pmid: 22890318
references.citation: Mol Cancer. 2013 Oct 05;12(1):114
references.pmid: 24093956
references.citation: Nat Rev Cancer. 2002 May;2(5):342-50
references.pmid: 12044010
references.citation: Cell Res. 2007 Dec;17(12):1020-9
references.pmid: 18040287