title: Easy washing of lysed cell plugs for bacterial typing by pulsed-field gel electrophoresis using simple equipment.
abstract: We designed and tested equipment to wash plugs following cell lysis in pulsed-field gel electrophoresis (PFGE). Our system can wash 30 plugs simultaneously in 1h using 15L of Tris-EDTA buffer, which makes plug washing for PFGE less labor-intensive.
authors.affiliation: Department of Health Science, Fukuoka Institute of Health and Environmental Sciences, 39 Mukaizano, Dazaifu, Fukuoka 818-0135, Japan. Electronic address: murakami@fihes.pref.fukuoka.jp.
authors.forename: Koichi
authors.initials: K
authors.lastname: Murakami
authors.affiliation: Department of Health Science, Fukuoka Institute of Health and Environmental Sciences, 39 Mukaizano, Dazaifu, Fukuoka 818-0135, Japan; Department of Health Sciences, Faculty of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan. Electronic address: noda-t8031@pref.fukuoka.lg.jp.
authors.forename: Tamie
authors.initials: T
authors.lastname: Noda
authors.affiliation: Department of Health Science, Fukuoka Institute of Health and Environmental Sciences, 39 Mukaizano, Dazaifu, Fukuoka 818-0135, Japan. Electronic address: e-maeda@fihes.pref.fukuoka.jp.
authors.forename: Eriko
authors.initials: E
authors.lastname: Maeda
authors.affiliation: Department of Health Science, Fukuoka Institute of Health and Environmental Sciences, 39 Mukaizano, Dazaifu, Fukuoka 818-0135, Japan. Electronic address: sera@fihes.pref.fukuoka.jp.
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Sera
authors.affiliation: Department of Health Sciences, Faculty of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan. Electronic address: shuuji@med.kyushu-u.ac.jp.
authors.forename: Shuji
authors.initials: S
authors.lastname: Fujimoto
mesh_terms: D015373:Bacterial Typing Techniques / Q000295:instrumentation*; D016521:Electrophoresis, Gel, Pulsed-Field / Q000295:instrumentation*; D017743:Equipment Reuse
keywords: E. coli; Plug washing; Pulsed-field gel electrophoresis; Salmonella