title: The role of P2X7 receptor in PC12 cells after exposure to oxygen-glucose deprivation.
abstract: Adenosine triphosphate (ATP) plays an important role in signal transmission via acting on P2X receptors. P2X7 receptor is involved in pathophysiological changes of ischemic diseases. The PC12 cell line is a popular model system to study sympathetic neuronal function. In this study, the effects of P2X7 on the viability or [Ca(2+)]i in PC12 cells after exposure to oxygen-glucose deprivation (OGD) were investigated. The results showed that the viability of PC12 cells was decreased under the condition of OGD. BzATP, a P2X7 agonist, decreased the viability, while P2X7 antagonist oxATP or P2X7 siRNA reversed the viability of PC12 cells under the condition of OGD. The expression levels of P2X7 mRNA and protein in PC12 cells were up-regulated under the condition of OGD or BzATP treatment. The expression levels of P2X7 mRNA and protein were significantly decreased in OGD PC12 cells, which were pretreated with oxATP or P2X7 siRNA. It was also found that oxATP or P2X7 siRNA effectively suppressed the increase of [Ca(2+)]i induced by OGD. P2X7 agonist ATP or BzATP enhanced the [Ca(2+)]i rise induced by OGD in PC12 cells. The [Ca(2+)]i peak induced by ATP or BzATP in OGD group was decreased by ERK inhibitor U0126. Therefore, P2X7 antagonists or P2X7 siRNA could depress the sympathetic neuronal damage induced by ischemia.
authors.affiliation: Department of Physiology, Medical School of Nanchang University, Nanchang 330006, PR China.
authors.forename: Bo
authors.initials: B
authors.lastname: Fan
authors.affiliation: Department of Physiology, Medical School of Nanchang University, Nanchang 330006, PR China.
authors.forename: Shuangmei
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Physiology, Medical School of Nanchang University, Nanchang 330006, PR China.
authors.forename: Changshui
authors.initials: C
authors.lastname: Xu
authors.affiliation: Department of Physiology, Medical School of Nanchang University, Nanchang 330006, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Physiology, Medical School of Nanchang University, Nanchang 330006, PR China.
authors.forename: Fanjun
authors.initials: F
authors.lastname: Kong
authors.affiliation: Department of Physiology, Medical School of Nanchang University, Nanchang 330006, PR China.
authors.forename: Guilin
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Physiology, Medical School of Nanchang University, Nanchang 330006, PR China.
authors.forename: Chunping
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Physiology, Medical School of Nanchang University, Nanchang 330006, PR China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Department of Physiology, Medical School of Nanchang University, Nanchang 330006, PR China.
authors.forename: Hong
authors.initials: H
authors.lastname: Xu
authors.affiliation: Department of Physiology, Medical School of Nanchang University, Nanchang 330006, PR China.
authors.forename: Shicheng
authors.initials: S
authors.lastname: Yu
authors.affiliation: Department of Physiology, Medical School of Nanchang University, Nanchang 330006, PR China.
authors.forename: Chaoran
authors.initials: C
authors.lastname: Zheng
authors.affiliation: Department of Physiology, Medical School of Nanchang University, Nanchang 330006, PR China.
authors.forename: Lichao
authors.initials: L
authors.lastname: Peng
authors.affiliation: Department of Physiology, Medical School of Nanchang University, Nanchang 330006, PR China.
authors.forename: Miaomiao
authors.initials: M
authors.lastname: Song
authors.affiliation: Department of Physiology, Medical School of Nanchang University, Nanchang 330006, PR China.
authors.forename: Bing
authors.initials: B
authors.lastname: Wu
authors.affiliation: Department of Physiology, Medical School of Nanchang University, Nanchang 330006, PR China.
authors.forename: Qiulan
authors.initials: Q
authors.lastname: Lv
authors.affiliation: Department of Physiology, Medical School of Nanchang University, Nanchang 330006, PR China.
authors.forename: Lifang
authors.initials: L
authors.lastname: Zou
authors.affiliation: Department of Physiology, Medical School of Nanchang University, Nanchang 330006, PR China.
authors.forename: Mofeng
authors.initials: M
authors.lastname: Ying
authors.affiliation: Department of Physiology, Medical School of Nanchang University, Nanchang 330006, PR China.
authors.forename: Xi
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Physiology, Medical School of Nanchang University, Nanchang 330006, PR China. Electronic address: liangsd@hotmail.com.
authors.forename: Shangdong
authors.initials: S
authors.lastname: Liang
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western; D002070:Butadienes / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D015687:Cell Hypoxia / Q000502:physiology*; D002470:Cell Survival / Q000502:physiology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D048049:Extracellular Signal-Regulated MAP Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D005947:Glucose / Q000172:deficiency*; D009570:Nitriles / Q000494:pharmacology; D016716:PC12 Cells; D058911:Purinergic P2X Receptor Agonists / Q000494:pharmacology; D058920:Purinergic P2X Receptor Antagonists / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D034741:RNA, Small Interfering; D051381:Rats; D058486:Receptors, Purinergic P2X7 / Q000235:genetics / Q000378:metabolism*
chemical_list: D002070:Butadienes; D004791:Enzyme Inhibitors; D009570:Nitriles; D058911:Purinergic P2X Receptor Agonists; D058920:Purinergic P2X Receptor Antagonists; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D058486:Receptors, Purinergic P2X7; C113580:U 0126; D000255:Adenosine Triphosphate; D048049:Extracellular Signal-Regulated MAP Kinases; D005947:Glucose; D002118:Calcium
keywords: Adenosine triphosphate; Intracellular Ca(2+); Oxygen–glucose deprivation; P2X(7) receptor; PC12 cell