title: A new flavonoid regulates angiogenesis and reactive oxygen species production.
abstract: The tumor vascular system, which is critical to the survival and growth of solid tumors, has been an attractive target for anticancer research. Building on studies that show that some flavonoids have anticancer vascular effects, we developed and analyzed the flavonoid derivative R24 [3, 6-bis (2-oxiranylmethoxy)-9H-xanthen-9-one]. A CAM assay revealed that R24 disrupted neovascular formation; fewer dendrites were detected and overall dendritic length was shorter in the R24-treated chicken embryos. The antiproliferative effect of R24 was measured by MTT assay in A549 (lung cancer), AsPC-1 (pancreatic cancer), HCT-116 (colorectal cancer), and PC-3 (prostate cancer) cell lines. R24 reduced proliferation with an IC50 of 3.44, 3.59, 1.22, and 11.83 μM, respectively. Cell-cycle analysis and Annexin-V/propidium iodide staining showed that R24 induced apoptosis. In addition, R24 regulated intracellular ROS production in a dose-dependent manner. CM-H2DCFDA staining indicated that intracellular ROS production increased with the R24 dose. In summary, we found that R24 exhibits potent antiangiogenic and antiproliferative effects, induces apoptosis, and promotes ROS production.
authors.affiliation: Department of Radiation Oncology, University of Florida, Cancer/Genetics Research Complex, 2033 Mowry Road, Suite 145, 103633, Gainesville, FL, 32610, USA.
authors.forename: Mei
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Department of Radiation Oncology, University of Florida, Cancer/Genetics Research Complex, 2033 Mowry Road, Suite 145, 103633, Gainesville, FL, 32610, USA.
authors.forename: Chaomei
authors.initials: C
authors.lastname: Liu
authors.affiliation: Department of Radiation Oncology, University of Florida, Cancer/Genetics Research Complex, 2033 Mowry Road, Suite 145, 103633, Gainesville, FL, 32610, USA.
authors.forename: Zhenhuan
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Radiation Oncology, University of Florida, Cancer/Genetics Research Complex, 2033 Mowry Road, Suite 145, 103633, Gainesville, FL, 32610, USA.
authors.forename: Shanmin
authors.initials: S
authors.lastname: Yang
authors.affiliation: Department of Radiation Oncology, University of Florida, Cancer/Genetics Research Complex, 2033 Mowry Road, Suite 145, 103633, Gainesville, FL, 32610, USA.
authors.forename: Bingrong
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Department of Radiation Oncology, University of Florida, Cancer/Genetics Research Complex, 2033 Mowry Road, Suite 145, 103633, Gainesville, FL, 32610, USA.
authors.forename: Liangjie
authors.initials: L
authors.lastname: Yin
authors.affiliation: Department of Radiation Oncology, University of Florida, Cancer/Genetics Research Complex, 2033 Mowry Road, Suite 145, 103633, Gainesville, FL, 32610, USA.
authors.forename: Steven
authors.initials: S
authors.lastname: Swarts
authors.affiliation: Department of Radiation Oncology, University of Florida, Cancer/Genetics Research Complex, 2033 Mowry Road, Suite 145, 103633, Gainesville, FL, 32610, USA.
authors.forename: Sadasivan
authors.initials: S
authors.lastname: Vidyasagar
authors.affiliation: Department of Radiation Oncology, University of Florida, Cancer/Genetics Research Complex, 2033 Mowry Road, Suite 145, 103633, Gainesville, FL, 32610, USA.
authors.forename: Lurong
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Radiation Oncology, University of Florida, Cancer/Genetics Research Complex, 2033 Mowry Road, Suite 145, 103633, Gainesville, FL, 32610, USA. pokunieff@ufl.edu.
authors.forename: Paul
authors.initials: P
authors.lastname: Okunieff
mesh_terms: D045744:Cell Line, Tumor; D005419:Flavonoids / Q000494:pharmacology*; D006801:Humans; D009369:Neoplasms / Q000098:blood supply*; D009389:Neovascularization, Pathologic / Q000517:prevention & control*; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D005419:Flavonoids; D017382:Reactive Oxygen Species