title: Collagen-lactoferrin fibrillar coatings enhance osteoblast proliferation and differentiation.
abstract: Lactoferrin is a milk-derived glycoprotein with anabolic effects on the bone tissue. In this study, artificial extracellular matrices (aECM) consisting of collagen type I fibrils formed in the presence of lactoferrin at two different concentrations (0.5 and 1 mg mL(-1) ) were prepared on the surface of poly(lactic-co-glycolic acid) (PLGA) foils. The aim of the study was to investigate the effects of aECM on the adhesion, growth and osteogenic differentiation of human osteoblast-like Saos-2 cells. On days 1 and 3 after seeding, higher numbers of cells were found on samples with collagen and collagen-lactoferrin coatings (particularly on those formed at the higher concentration of lacroferrin) than on control microscopic glass coverslips. Cells on coatings formed in the presence of lactoferrin had more numerous and better developed vinculin-containing focal adhesion plaques. On day 7, cells on coatings with and without lactoferrin produced significantly higher levels of osteocalcin than cells on control polystyrene cell culture dishes, the highest average values being found on samples with the lower concentration of lactoferrin. Expression of collagen I and alkaline phosphatase was on a similar level in cells on all tested samples and control polystyrene. Thus, lactoferrin promotes adhesion, growth and osteogenic differentiation of Saos-2 cells and is promising as a bone implant coating component.
authors.affiliation: Department of Biomaterials and Tissue Engineering, Institute of Physiology, Academy of Sciences of the Czech Republic, Videnska 1083, 14220 Prague 4, Czech Republic.
authors.forename: Marta
authors.initials: M
authors.lastname: Vandrovcova
authors.forename: Timothy E L
authors.initials: TE
authors.lastname: Douglas
authors.forename: Sascha
authors.initials: S
authors.lastname: Heinemann
authors.forename: Dieter
authors.initials: D
authors.lastname: Scharnweber
authors.forename: Peter
authors.initials: P
authors.lastname: Dubruel
authors.forename: Lucie
authors.initials: L
authors.lastname: Bacakova
mesh_terms: D023181:Antimicrobial Cationic Peptides / Q000737:chemistry; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D003094:Collagen / Q000737:chemistry*; D006801:Humans; D007781:Lactoferrin / Q000737:chemistry*; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*
chemical_list: D023181:Antimicrobial Cationic Peptides; D020099:Coated Materials, Biocompatible; C045866:peptide-Gly-Leu-amide; D003094:Collagen; D007781:Lactoferrin
keywords: artificial extracellular matrix; bone cells; coating; collagen; lactoferrin