title: Synchrotron based infrared imaging and spectroscopy via focal plane array on live fibroblasts in D2O enriched medium.
abstract: We successfully tested the viability of using synchrotron-based full-field infrared imaging to study biochemical processes inside living cells. As a model system, we studied fibroblast cells exposed to a medium highly enriched with D2O. We could show that the experimental technique allows us to reproduce at the cellular level measurements that are normally performed on purified biological molecules. We can obtain information about lipid conformation and distribution, kinetics of hydrogen/deuterium exchange, and the formation of concentration gradients of H and O isotopes in water that are associated with cell metabolism. The implementation of the full field technique in a sequential imaging format gives a description of cellular biochemistry and biophysics that contains both spatial and temporal information.
authors.affiliation: Paul Scherrer Institut, Villigen-PSI, CH-5232, Switzerland. Electronic address: quaroni@bluewin.ch.
authors.forename: Luca
authors.initials: L
authors.lastname: Quaroni
authors.affiliation: Paul Scherrer Institut, Villigen-PSI, CH-5232, Switzerland.
authors.forename: Theodora
authors.initials: T
authors.lastname: Zlateva
authors.affiliation: Paul Scherrer Institut, Villigen-PSI, CH-5232, Switzerland.
authors.forename: Blagoj
authors.initials: B
authors.lastname: Sarafimov
authors.affiliation: Pacific Northwest National Laboratory, Richland, WA 99354, USA.
authors.forename: Helen W
authors.initials: HW
authors.lastname: Kreuzer
authors.affiliation: Diamond Light Source, Harwell Campus, Chilton-Didcot, Oxon OX11 0DE, UK.
authors.forename: Katia
authors.initials: K
authors.lastname: Wehbe
authors.affiliation: Michigan State University, Department of Biochemistry & Molecular Biology, East Lansing, MI 48824, USA.
authors.forename: Eric L
authors.initials: EL
authors.lastname: Hegg
authors.affiliation: Diamond Light Source, Harwell Campus, Chilton-Didcot, Oxon OX11 0DE, UK.
authors.forename: Gianfelice
authors.initials: G
authors.lastname: Cinque
mesh_terms: D000818:Animals; D002470:Cell Survival; D041961:Deuterium Exchange Measurement; D017666:Deuterium Oxide / Q000737:chemistry*; D005347:Fibroblasts / Q000166:cytology*; D006652:Histological Techniques; D007700:Kinetics; D008099:Liver / Q000166:cytology*; D051379:Mice; D041681:NIH 3T3 Cells; D017550:Spectroscopy, Fourier Transform Infrared; D017356:Synchrotrons
chemical_list: D017666:Deuterium Oxide
keywords: Cell lipid; Full-field IR microscopy; Infrared spectromicroscopy; Isotope tracer; Single cell; Synchrotron radiation