title: Histologic findings of recipient corneas obtained via deep anterior lamellar keratoplasty.
abstract: OBJECTIVE
  We sought to investigate the histologic findings of recipient corneas obtained via deep anterior lamellar keratoplasty (DALK).
  
  
  METHODS
  The histology of three recipient corneas obtained from patients during DALK was investigated. In all cases, the Descemet membrane was successfully exposed without any perforation during surgery. The isolated corneal tissue was stained with hematoxylin-eosin and periodic acid-Schiff. In two cases, the tissues were examined using a transmission electron microscope.
  
  
  RESULTS
  In one cornea obtained via DALK, only the corneal stroma was observed, and the Descemet membrane was not confirmed. In another case, the recipient cornea was detached within the Descemet membrane. In the third cornea, the banded layer membrane was partially confirmed.
  
  
  CONCLUSIONS
  These findings suggest that the recipient corneas separated at different layers during the DALK procedure. With our surgical technique, the detachment of the Descemet membrane may occur at a mechanically weak segment. This separation site may not be between the Descemet membrane and the corneal stroma.
authors.affiliation: Department of Ophthalmology, Dokkyo Medical University School of Medicine, 880 Mibu, Shimotsuga, Tochigi, 321-0293, Japan.
authors.forename: Shimpei
authors.initials: S
authors.lastname: Ishimaru
authors.forename: Tadashi
authors.initials: T
authors.lastname: Senoo
authors.forename: Norihito
authors.initials: N
authors.lastname: Gotoh
authors.forename: Akira
authors.initials: A
authors.lastname: Kurosu
authors.forename: Keizo
authors.initials: K
authors.lastname: Chiba
mesh_terms: D000368:Aged; D003315:Cornea / Q000473:pathology*; D003316:Corneal Diseases / Q000601:surgery; D003319:Corneal Stroma / Q000473:pathology* / Q000601:surgery; D016039:Corneal Transplantation; D003886:Descemet Membrane / Q000601:surgery / Q000648:ultrastructure*; D005260:Female; D006801:Humans; D046529:Microscopy, Electron, Transmission; D066027:Transplant Recipients; D014792:Visual Acuity / Q000502:physiology
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