title: Tripartite motif-containing protein 30 modulates TCR-activated proliferation and effector functions in CD4+ T cells.
abstract: To avoid excessive activation, immune signals are tightly controlled by diverse inhibitory proteins. TRIM30, a tripartite motif (TRIM)-containing protein is one of such inhibitors known to function in macrophages. To define the roles of TRIM30, we generated Trim30 knockout (Trim30-/-) mice. Trim30 deletion caused no major developmental defects in any organs, nor showed any discernable defect in the activation of macrophages. But, Trim30-/- mice showed increased CD4/CD8 ratio when aged and Trim30-/- CD4+ T cells exhibited an abnormal response upon TCR activation, in particular in the absence of a costimulatory signal. Adoptive transfer of wild-type and Trim30-/- CD4+ T cells together into lymphopenic hosts confirmed higher proliferation of the Trim30-/- CD4+ T cells in vivo. Despite the enhanced proliferation, Trim30-/- T cells showed decreased levels of NF-κB activation and IL-2 production compared to wild-type cells. These results indicate a distinct requirement for TRIM30 in modulation of NF-κB activation and cell proliferation induced by TCR stimulation.
authors.affiliation: Department of Biochemistry, College of Life Science and Technology, Yonsei University, Seoul, Korea.
authors.forename: Un Yung
authors.initials: UY
authors.lastname: Choi
authors.affiliation: Department of Integrated Omics for Biomedical Science, WCU Program of Graduate School, Yonsei University, Seoul, Korea.
authors.forename: Ji Yeon
authors.initials: JY
authors.lastname: Hur
authors.affiliation: Department of Biochemistry, College of Life Science and Technology, Yonsei University, Seoul, Korea.
authors.forename: Myeong Sup
authors.initials: MS
authors.lastname: Lee
authors.affiliation: Department of Integrated Omics for Biomedical Science, WCU Program of Graduate School, Yonsei University, Seoul, Korea.
authors.forename: Quanri
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Biochemistry, College of Life Science and Technology, Yonsei University, Seoul, Korea.
authors.forename: Won Young
authors.initials: WY
authors.lastname: Choi
authors.affiliation: Department of Biochemistry, College of Life Science and Technology, Yonsei University, Seoul, Korea.
authors.forename: Lark Kyun
authors.initials: LK
authors.lastname: Kim
authors.affiliation: Department of Biochemistry, College of Life Science and Technology, Yonsei University, Seoul, Korea.
authors.forename: Wook-Bin
authors.initials: WB
authors.lastname: Lee
authors.affiliation: Division of Life and Pharmaceutical Sciences, Ewha Women's University, Seoul, Korea.
authors.forename: Goo Taeg
authors.initials: GT
authors.lastname: Oh
authors.affiliation: Department of Biochemistry, College of Life Science and Technology, Yonsei University, Seoul, Korea; Department of Integrated Omics for Biomedical Science, WCU Program of Graduate School, Yonsei University, Seoul, Korea.
authors.forename: Young-Joon
authors.initials: YJ
authors.lastname: Kim
mesh_terms: D000367:Age Factors; D000818:Animals; D016516:CD4-CD8 Ratio; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D002453:Cell Cycle / Q000235:genetics; D018398:Homeodomain Proteins / Q000235:genetics; D008213:Lymphocyte Activation / Q000235:genetics* / Q000276:immunology*; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D016328:NF-kappa B / Q000378:metabolism; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D018398:Homeodomain Proteins; D016328:NF-kappa B; D011948:Receptors, Antigen, T-Cell; C586979:TRIM3 protein, mouse; C064658:RAG-1 protein
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