title: Anti-fibrosis effects of Huisheng oral solution in CCl4-induced hepatic fibrosis in rat.
abstract: OBJECTIVE
  Some gradient of Huisheng oral solution (HOS) has been reported to have anti-fibrosis activity. This study was designed to investigate whether HOS could inhibit liver fibrosis and to elucidate its molecular mechanism of action.
  
  
  METHODS
  Hepatic fibrosis model in rat was induced by subcutaneous injection of CCl4. Rats in the treatment group were administrated with HOS intragastrically. Hematoxylin and eosin (H and E) staining and Masson's trichrome staining were used to examine the changes in liver pathology. Levels of ALT, AST, LDH, hyaluronic acid (HA) and laminin (LN) in serum and hydroxyproline (Hyp) in liver were detected by biochemical examination and radioimmunoassay, respectively. The expression and distribution of Smad3, TGF-β1, α-SMA and TIMP-1 were observed and the active TGF-β1 was tested.
  
  
  RESULTS
  Our data demonstrated that HOS alleviated CCl4-induced collagen deposition in liver tissue, improved liver condition and liver function in rats. HOS also significantly reduced the expression and distribution of Smad3, TGF-β1, α-SMA and TIMP-1 as well as decreased active TGF-β1.
  
  
  CONCLUSIONS
  This study revealed that HOS attenuates the development of liver fibrosis through suppressing the TGF-β1 pathway. It provides us a new approach to treatment of liver fibrosis.
authors.affiliation: Department of Infectious Disease, Anhui Provincial Hospital, Hefei, China.
authors.forename: Wenting
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Neurology, Anhui Provincial Hospital, Hefei, China.
authors.forename: Yuanbo
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Infectious Disease, Anhui Provincial Hospital, Hefei, China.
authors.forename: Chuanlong
authors.initials: C
authors.lastname: Zhu
authors.affiliation: Department of Respiratory and Critical Medicine, Zhengzhou University, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Infectious Disease, Anhui Provincial Hospital, Hefei, China.
authors.forename: Rentao
authors.initials: R
authors.lastname: Gao
authors.affiliation: Central Lab, Anhui Provincial Hospital, Hefei, China.
authors.forename: Quan
authors.initials: Q
authors.lastname: Wu
mesh_terms: D000818:Animals; D002251:Carbon Tetrachloride / Q000633:toxicity*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000627:therapeutic use*; D008099:Liver / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D008103:Liver Cirrhosis / Q000139:chemically induced / Q000276:immunology / Q000517:prevention & control*; D008111:Liver Function Tests; D008297:Male; D028321:Plant Preparations / Q000008:administration & dosage / Q000627:therapeutic use*; D017208:Rats, Wistar; D053773:Transforming Growth Factor beta1 / Q000037:antagonists & inhibitors / Q000276:immunology
chemical_list: D004365:Drugs, Chinese Herbal; C587426:Huisheng oral solution; D028321:Plant Preparations; D053773:Transforming Growth Factor beta1; D002251:Carbon Tetrachloride
keywords: Huisheng oral solution; TGF-β1; liver fibrosis
references.citation: Dig Liver Dis. 2011 Jul;43(7):559-66
references.pmid: 21444256
references.citation: J Ginseng Res. 2013 Mar;37(1):45-53
references.pmid: 23717156
references.citation: World J Hepatol. 2012 Dec 27;4(12):356-64
references.pmid: 23355913
references.citation: ISRN Gastroenterol. 2012;2012:762920
references.pmid: 22577570
references.citation: Prog Mol Biol Transl Sci. 2012;105:371-409
references.pmid: 22137437
references.citation: Best Pract Res Clin Gastroenterol. 2011 Apr;25(2):305-17
references.pmid: 21497747
references.citation: Cell Prolif. 2005 Oct;38(5):287-99
references.pmid: 16202037
references.citation: Indian J Pharmacol. 2013 Jul-Aug;45(4):359-64
references.pmid: 24014911
references.citation: Eur J Gastroenterol Hepatol. 2014 Feb;26(2):164-73
references.pmid: 23903851
references.citation: Liver Int. 2005 Dec;25(6):1224-32
references.pmid: 16343076
references.citation: Hepatology. 2012 Nov;56(5):1870-82
references.pmid: 22576182
references.citation: Hepatogastroenterology. 2012 Sep;59(118):1960-3
references.pmid: 22369740
references.citation: Mol Med Rep. 2013 Oct;8(4):1023-8
references.pmid: 23934318
references.citation: Hepatology. 1994 Aug;20(2):349-55
references.pmid: 8045495
references.citation: J Hepatol. 2013 Apr;58(4):736-42
references.pmid: 23183523
references.citation: Clin Nutr. 2013 Apr;32(2):252-8
references.pmid: 22835810