title: Purification of a polysaccharide from Boschniakia rossica and its synergistic antitumor effect combined with 5-Fluorouracil.
abstract: Current study we purified a polysaccharide (BRP) from Boschniakia rossica and the antitumor effects of BRP alone or combined with 5-Fluorouracil (5-FU) was examined in S180 tumor bearing mice by intragastric administration. The high performance size-exclusion chromatography (HPGEC) analysis showed that BRP was a homogeneous polysaccharide and had a molecular weight of 2.2 × 10(4) Da. Total carbohydrate content in BRP was determined to be 96.9%, without the presence of protein and nucleic acid. BRP alone or combined with 5-FU could significantly inhibit Sarcoma-180 (S180) tumor growth and increase the spleen index in a dose-dependent manner. Meanwhile a synergistic effect was observed in boosting various immunity functions when the tumor bearing mice receiving BRP plus 5-FU administration, such as stimulating lymphocytes proliferation, increasing NK cell cytotoxicity, enhancing serum interleukin-2 (IL-2) and interferon-gamma (TNF-γ) secretion, as well as augmenting CD4+ and CD8+ spleen T lymphocytes subsets. The results showed that BRP combined with 5-FU presented synergistic effects on antitumor activity in tumor bearing mice. In conclusion, the combination of BRP may boost the suppressed immunity in tumor bearing mice subject to 5-FU chemotherapy, and could serve as a new, promising approach for cancer treatment.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, The Second Hospital, Xi'an Jiao Tong University, Xi'an 710004, China.
authors.forename: Zhenghui
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, The Second Hospital, Xi'an Jiao Tong University, Xi'an 710004, China.
authors.forename: Baojun
authors.initials: B
authors.lastname: Wu
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, The Second Hospital, Xi'an Jiao Tong University, Xi'an 710004, China.
authors.forename: Xianghong
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, The Second Hospital, Xi'an Jiao Tong University, Xi'an 710004, China.
authors.forename: Min
authors.initials: M
authors.lastname: Xu
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, The Second Hospital, Xi'an Jiao Tong University, Xi'an 710004, China.
authors.forename: Huimin
authors.initials: H
authors.lastname: Chang
authors.affiliation: Department of Biological Science and Bioengineering, Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiao Tong University, Xi'an 710049, China.
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Lu
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, The Second Hospital, Xi'an Jiao Tong University, Xi'an 710004, China.
authors.forename: Xiaoyong
authors.initials: X
authors.lastname: Ren
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D005472:Fluorouracil / Q000008:administration & dosage; D007376:Interleukin-2 / Q000378:metabolism; D007694:Killer Cells, Natural / Q000187:drug effects; D008297:Male; D008813:Mice, Inbred ICR; D009369:Neoplasms / Q000188:drug therapy / Q000473:pathology; D031670:Orobanchaceae; D008517:Phytotherapy; D011134:Polysaccharides / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology; D013154:Spleen / Q000166:cytology / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D007376:Interleukin-2; D011134:Polysaccharides; D014409:Tumor Necrosis Factor-alpha; D005472:Fluorouracil