title: Molecular magnetic resonance probe targeting VEGF165: preparation and in vitro and in vivo evaluation.
abstract: A new method for imaging the tumor human vascular endothelial growth factor 165 (VEGF 165) is presented. A magnetic resonance imaging (MRI) probe was prepared by crosslinking ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles to the aptamer for tumor vascular endothelial growth factor 165 (VEGF165-aptamer). The molecular probe was evaluated for its in vitro and in vivo activities toward VEGF165. Enzyme-linked immunosorbent assay showed that the VEGF165-aptamer-USPIO nanoparticles conjugate specifically binds to VEGF165 in vitro. A cell proliferation test showed that VEGF165-aptamer-USPIO seems to block the proliferation of human umbilical vein endothelial cells induced by free VEGF165, suggesting that VEGF165 is an effective target of this molecular probe. In xenograft mice carrying liver cancer that expresses VEGF165, T2-weighted imaging of the tumor displayed marked negative enhancement 3 h after the intravenous administration of VEGF165-aptamer-USPIO. The enhancement disappeared 6 h after administration of the probe. These results suggest the targeted imaging effect of VEGF165-aptamer-USPIO probe in vivo for VEGF165-expressing tumors. This is the first report of a targeted MRI molecular probe based on USPIO and VEGF165-aptamer.
authors.affiliation: Department of Radiology and Cancer Institute, Hainan Medical College Hospital, Haikou City, Hainan Province, 570102, China.
authors.forename: Xiao-Guang
authors.initials: XG
authors.lastname: You
authors.forename: Rong
authors.initials: R
authors.lastname: Tu
authors.forename: Ming-Li
authors.initials: ML
authors.lastname: Peng
authors.forename: Yu-Jie
authors.initials: YJ
authors.lastname: Bai
authors.forename: Mingqian
authors.initials: M
authors.lastname: Tan
authors.forename: Han-Jian
authors.initials: HJ
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Guan
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Wen
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide; D052158:Aptamers, Peptide / Q000737:chemistry; D045744:Cell Line, Tumor; D003287:Contrast Media / Q000737:chemistry; D003911:Dextrans / Q000737:chemistry; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D008279:Magnetic Resonance Imaging; D058185:Magnetite Nanoparticles / Q000737:chemistry; D051379:Mice; D015335:Molecular Probes / Q000737:chemistry*; D042461:Vascular Endothelial Growth Factor A / Q000302:isolation & purification*
chemical_list: D052157:Aptamers, Nucleotide; D052158:Aptamers, Peptide; D003287:Contrast Media; D003911:Dextrans; D058185:Magnetite Nanoparticles; D015335:Molecular Probes; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; C097921:ferumoxtran-10
keywords: (USPIO); MRI; VEGF165; aptamer; liver cancer; targeted molecular probe