title: Osthole prevents intestinal ischemia-reperfusion-induced lung injury in a rodent model.
abstract: BACKGROUND
  Intestinal ischemia-reperfusion (II/R) is associated with high morbidity and mortality. The aim of this study was to investigate the effects of osthole on lung injury and mortality induced by II/R.
  
  
  METHODS
  A rat model of II/R was induced by clamping the superior mesenteric artery for 90 min followed by reperfusion for 240 min. Osthole was administrated intraperitoneally at 30 min before intestinal ischemia (10 or 50 mg/kg). The survival rate and mean arterial pressure were observed. Blood samples were obtained for blood gas analyses. Lung injury was assessed by the histopathologic changes (hematoxylin and eosin staining), lung wet-to-dry weight ratio, and pulmonary permeability index. The levels of reactive oxygen species, malondialdehyde, interleukin 6, and tumor necrosis factor α, as well as the activities of superoxide dismutase and myeloperoxidase in lung were measured.
  
  
  RESULTS
  The survival rate, ratio of arterial oxygen tension to fraction of inspired oxygen, and mean arterial pressure decreased significantly after II/R. Results also indicated that II/R-induced severe lung injury evidenced by increase in pathologic scores, lung wet-to-dry weight ratio, and pulmonary permeability index, which was accompanied by increases in the levels of pulmonary reactive oxygen species, malondialdehyde, interleukin 6, tumor necrosis factor α, and the pulmonary myeloperoxidase activity and a decrease in superoxide dismutase activity. Osthole could significantly ameliorate lung injury and improve the previously mentioned variables.
  
  
  CONCLUSIONS
  These findings indicated that osthole could attenuate the lung injury induced by II/R in rats, at least in part, by inhibiting inflammatory response and oxidative stress.
authors.affiliation: Department of Anesthesiology, Affiliated Hospital of Luzhou Medical College, Luzhou, People's Republic of China.
authors.forename: Li-Qun
authors.initials: LQ
authors.lastname: Mo
authors.affiliation: Department of Traditional Chinese Medicine, Affiliated Hospital of Luzhou Medical College, Luzhou, People's Republic of China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Anesthesiology, Affiliated Hospital of Luzhou Medical College, Luzhou, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Song
authors.affiliation: Department of Anesthesiology, Affiliated Hospital of Luzhou Medical College, Luzhou, People's Republic of China.
authors.forename: Gang-Ming
authors.initials: GM
authors.lastname: Wu
authors.affiliation: Department of Anesthesiology, Affiliated Hospital of Luzhou Medical College, Luzhou, People's Republic of China.
authors.forename: Ni
authors.initials: N
authors.lastname: Tang
authors.affiliation: Department of Anesthesiology, Affiliated Hospital of Luzhou Medical College, Luzhou, People's Republic of China.
authors.forename: Ying-Ying
authors.initials: YY
authors.lastname: Zhang
authors.affiliation: Department of Anesthesiology, Affiliated Hospital of Luzhou Medical College, Luzhou, People's Republic of China.
authors.forename: Xiao-Bin
authors.initials: XB
authors.lastname: Wang
authors.affiliation: Department of Anesthesiology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, People's Republic of China.
authors.forename: Ke-Xuan
authors.initials: KX
authors.lastname: Liu
authors.affiliation: Department of Anesthesiology, Affiliated Hospital of Luzhou Medical College, Luzhou, People's Republic of China. Electronic address: zhoujun800615@163.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhou
mesh_terms: D055371:Acute Lung Injury / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D002121:Calcium Channel Blockers / Q000494:pharmacology / Q000627:therapeutic use*; D003374:Coumarins / Q000494:pharmacology / Q000627:therapeutic use*; D004195:Disease Models, Animal; D015850:Interleukin-6 / Q000378:metabolism; D008168:Lung / Q000378:metabolism / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009929:Organ Size / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D009195:Peroxidase / Q000378:metabolism; D011659:Pulmonary Gas Exchange / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D015427:Reperfusion Injury / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D013482:Superoxide Dismutase / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D002121:Calcium Channel Blockers; D003374:Coumarins; D015850:Interleukin-6; D017382:Reactive Oxygen Species; D014409:Tumor Necrosis Factor-alpha; D008315:Malondialdehyde; D009195:Peroxidase; D013482:Superoxide Dismutase; C046627:osthol
keywords: Inflammatory response; Intestinal ischemia–reperfusion; Lung injury; Osthole; Oxidative stress