title: Expression of a truncated ATHB17 protein in maize increases ear weight at silking.
abstract: ATHB17 (AT2G01430) is an Arabidopsis gene encoding a member of the α-subclass of the homeodomain leucine zipper class II (HD-Zip II) family of transcription factors. The ATHB17 monomer contains four domains common to all class II HD-Zip proteins: a putative repression domain adjacent to a homeodomain, leucine zipper, and carboxy terminal domain. However, it also possesses a unique N-terminus not present in other members of the family. In this study we demonstrate that the unique 73 amino acid N-terminus is involved in regulation of cellular localization of ATHB17. The ATHB17 protein is shown to function as a transcriptional repressor and an EAR-like motif is identified within the putative repression domain of ATHB17. Transformation of maize with an ATHB17 expression construct leads to the expression of ATHB17Δ113, a truncated protein lacking the first 113 amino acids which encodes a significant portion of the repression domain. Because ATHB17Δ113 lacks the repression domain, the protein cannot directly affect the transcription of its target genes. ATHB17Δ113 can homodimerize, form heterodimers with maize endogenous HD-Zip II proteins, and bind to target DNA sequences; thus, ATHB17Δ113 may interfere with HD-Zip II mediated transcriptional activity via a dominant negative mechanism. We provide evidence that maize HD-Zip II proteins function as transcriptional repressors and that ATHB17Δ113 relieves this HD-Zip II mediated transcriptional repression activity. Expression of ATHB17Δ113 in maize leads to increased ear size at silking and, therefore, may enhance sink potential. We hypothesize that this phenotype could be a result of modulation of endogenous HD-Zip II pathways in maize.
authors.affiliation: Monsanto Company, St. Louis, Missouri, United States of America.
authors.forename: Elena A
authors.initials: EA
authors.lastname: Rice
authors.affiliation: Monsanto Company, St. Louis, Missouri, United States of America.
authors.forename: Abha
authors.initials: A
authors.lastname: Khandelwal
authors.affiliation: Mendel Biotechnology Inc., Hayward, California, United States of America.
authors.forename: Robert A
authors.initials: RA
authors.lastname: Creelman
authors.affiliation: Monsanto Company, St. Louis, Missouri, United States of America.
authors.forename: Cara
authors.initials: C
authors.lastname: Griffith
authors.affiliation: Monsanto Company, St. Louis, Missouri, United States of America.
authors.forename: Jeffrey E
authors.initials: JE
authors.lastname: Ahrens
authors.affiliation: Monsanto Company, St. Louis, Missouri, United States of America.
authors.forename: J Philip
authors.initials: JP
authors.lastname: Taylor
authors.affiliation: Monsanto Company, St. Louis, Missouri, United States of America.
authors.forename: Lesley R
authors.initials: LR
authors.lastname: Murphy
authors.affiliation: Monsanto Company, St. Louis, Missouri, United States of America.
authors.forename: Siva
authors.initials: S
authors.lastname: Manjunath
authors.affiliation: Monsanto Company, St. Louis, Missouri, United States of America.
authors.forename: Rebecca L
authors.initials: RL
authors.lastname: Thompson
authors.affiliation: Monsanto Company, St. Louis, Missouri, United States of America.
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Lingard
authors.affiliation: Monsanto Company, St. Louis, Missouri, United States of America.
authors.forename: Stephanie L
authors.initials: SL
authors.lastname: Back
authors.affiliation: Monsanto Company, St. Louis, Missouri, United States of America.
authors.forename: Huachun
authors.initials: H
authors.lastname: Larue
authors.affiliation: Dupont-Pioneer Hi-Bred International, Waipahu, Hawaii, United States of America.
authors.forename: Bonnie R
authors.initials: BR
authors.lastname: Brayton
authors.affiliation: Mendel Biotechnology Inc., Hayward, California, United States of America.
authors.forename: Amanda J
authors.initials: AJ
authors.lastname: Burek
authors.affiliation: Dupont-Pioneer Hi-Bred International, Hayward, California, United States of America.
authors.forename: Shiv
authors.initials: S
authors.lastname: Tiwari
authors.affiliation: ABCAM, Burlingame, California, United States of America.
authors.forename: Luc
authors.initials: L
authors.lastname: Adam
authors.affiliation: Monsanto Company, St. Louis, Missouri, United States of America.
authors.forename: James A
authors.initials: JA
authors.lastname: Morrell
authors.affiliation: Monsanto Company, St. Louis, Missouri, United States of America.
authors.forename: Rico A
authors.initials: RA
authors.lastname: Caldo
authors.affiliation: Monsanto Company, Cambridge, Massachusetts, United States of America.
authors.forename: Qing
authors.initials: Q
authors.lastname: Huai
authors.affiliation: Monsanto Company, St. Louis, Missouri, United States of America.
authors.forename: Jean-Louis K
authors.initials: JL
authors.lastname: Kouadio
authors.affiliation: Monsanto Company, St. Louis, Missouri, United States of America.
authors.forename: Rosemarie
authors.initials: R
authors.lastname: Kuehn
authors.affiliation: Monsanto Company, St. Louis, Missouri, United States of America.
authors.forename: Anagha M
authors.initials: AM
authors.lastname: Sant
authors.affiliation: Monsanto Company, St. Louis, Missouri, United States of America.
authors.forename: William J
authors.initials: WJ
authors.lastname: Wingbermuehle
authors.affiliation: Monsanto Company, St. Louis, Missouri, United States of America.
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Sala
authors.affiliation: Monsanto Company, St. Louis, Missouri, United States of America.
authors.forename: Matt
authors.initials: M
authors.lastname: Foster
authors.affiliation: Monsanto Company, St. Louis, Missouri, United States of America.
authors.forename: Josh D
authors.initials: JD
authors.lastname: Kinser
authors.affiliation: Monsanto Company, St. Louis, Missouri, United States of America.
authors.forename: Radha
authors.initials: R
authors.lastname: Mohanty
authors.affiliation: Monsanto Company, St. Louis, Missouri, United States of America.
authors.forename: Dongming
authors.initials: D
authors.lastname: Jiang
authors.affiliation: Monsanto Company, St. Louis, Missouri, United States of America.
authors.forename: Todd E
authors.initials: TE
authors.lastname: Ziegler
authors.affiliation: Monsanto Company, St. Louis, Missouri, United States of America.
authors.forename: Mingya G
authors.initials: MG
authors.lastname: Huang
authors.affiliation: Monsanto Company, Bangalore, Karnataka, India.
authors.forename: Saritha V
authors.initials: SV
authors.lastname: Kuriakose
authors.affiliation: Monsanto Company, St. Louis, Missouri, United States of America.
authors.forename: Kyle
authors.initials: K
authors.lastname: Skottke
authors.affiliation: Mendel Biotechnology Inc., Hayward, California, United States of America.
authors.forename: Peter P
authors.initials: PP
authors.lastname: Repetti
authors.affiliation: Mendel Biotechnology Inc., Hayward, California, United States of America.
authors.forename: T Lynne
authors.initials: TL
authors.lastname: Reuber
authors.affiliation: Monsanto Company, St. Louis, Missouri, United States of America.
authors.forename: Thomas G
authors.initials: TG
authors.lastname: Ruff
authors.affiliation: Monsanto Company, St. Louis, Missouri, United States of America.
authors.forename: Marie E
authors.initials: ME
authors.lastname: Petracek
authors.affiliation: Monsanto Company, St. Louis, Missouri, United States of America.
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Loida
mesh_terms: D021581:Active Transport, Cell Nucleus; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D001835:Body Weight / Q000235:genetics; D002467:Cell Nucleus / Q000378:metabolism; D016384:Consensus Sequence; D015870:Gene Expression; D008969:Molecular Sequence Data; D055503:Protein Multimerization; D020836:Protein Structure, Quaternary; D011523:Protoplasts / Q000378:metabolism; D012098:Reproduction; D017384:Sequence Deletion / Q000235:genetics*; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D014158:Transcription, Genetic; D003313:Zea mays / Q000166:cytology / Q000235:genetics* / Q000254:growth & development* / Q000502:physiology
chemical_list: D029681:Arabidopsis Proteins; C000588571:HB17 protein, Arabidopsis; D014157:Transcription Factors
references.citation: Nat Protoc. 2007;2(7):1565-72
references.pmid: 17585298
references.citation: Genes Dev. 1994 Jun 15;8(12):1388-99
references.pmid: 7926739
references.citation: Nature. 2001 Jun 7;411(6838):709-13
references.pmid: 11395776
references.citation: Plant J. 2010 Jul 1;63(1):155-66
references.pmid: 20409000
references.citation: Cell Mol Life Sci. 2008 May;65(9):1390-402
references.pmid: 18264803
references.citation: Trends Plant Sci. 2011 Oct;16(10):541-9
references.pmid: 21723179
references.citation: J Exp Bot. 2013 Nov;64(14):4479-90
references.pmid: 24006420
references.citation: Proc Natl Acad Sci U S A. 2005 Apr 5;102(14):5280-5
references.pmid: 15781873
references.citation: Plant Signal Behav. 2012 Nov;7(11):1382-7
references.pmid: 22918502
references.citation: Plant Mol Biol. 2008 Jan;66(1-2):87-103
references.pmid: 17999151
references.citation: EMBO Rep. 2011 Jan;12(1):35-42
references.pmid: 21151039
references.citation: Plant Cell. 2005 Jul;17(7):1908-25
references.pmid: 15937229
references.citation: Bioinformatics. 2007 Nov 1;23(21):2947-8
references.pmid: 17846036
references.citation: Cell. 1990 Nov 2;63(3):579-90
references.pmid: 1977522
references.citation: Plant Mol Biol. 2008 Nov;68(4-5):465-78
references.pmid: 18758690
references.citation: Trends Plant Sci. 2008 Mar;13(3):137-44
references.pmid: 18314376
references.citation: EMBO J. 1991 Jul;10(7):1787-91
references.pmid: 1675603
references.citation: Nucleic Acids Res. 2002 Jan 1;30(1):207-10
references.pmid: 11752295
references.citation: Plant Cell. 2007 Dec;19(12):4111-9
references.pmid: 18156219
references.citation: Plant Mol Biol. 2002 Aug;49(6):601-10
references.pmid: 12081368
references.citation: Plant Cell. 1994 Apr;6(4):545-59
references.pmid: 8205004
references.citation: Plant Mol Biol. 2004 Mar;54(4):549-69
references.pmid: 15316289
references.citation: Plant Mol Biol. 1990 Jul;15(1):65-79
references.pmid: 2103444
references.citation: Plant J. 1997 Feb;11(2):263-76
references.pmid: 9076993
references.citation: Plant J. 1998 Aug;15(3):413-21
references.pmid: 9750352
references.citation: Plant Cell Physiol. 2002 Oct;43(10):1146-53
references.pmid: 12407194
references.citation: Proteins. 1991;11(2):95-110
references.pmid: 1946347
references.citation: Methods Mol Biol. 2009;526:47-58
references.pmid: 19378003
references.citation: J Biol Chem. 2012 Sep 7;287(37):31482-93
references.pmid: 22801428
references.citation: Plant Cell. 2007 Nov;19(11):3379-90
references.pmid: 18055602
references.citation: Development. 1999 Oct;126(19):4235-45
references.pmid: 10477292
references.citation: Plant Cell. 1996 Dec;8(12):2155-68
references.pmid: 8989876
references.citation: Plant Cell Physiol. 2008 Oct;49(10):1522-35
references.pmid: 18718934
references.citation: Plant J. 1994 Mar;5(3):421-7
references.pmid: 8180625
references.citation: Development. 2013 May;140(10):2118-29
references.pmid: 23578926
references.citation: Plant Cell. 2006 Sep;18(9):2157-71
references.pmid: 16891401
references.citation: Mol Cells. 2013 Feb;35(2):125-33
references.pmid: 23456334
references.citation: Plant Physiol. 2006 Aug;141(4):1363-75
references.pmid: 16778018
references.citation: Curr Biol. 2003 Oct 14;13(20):1768-74
references.pmid: 14561401
references.citation: Methods Mol Biol. 2006;323:237-44
references.pmid: 16739582
references.citation: J Biol Chem. 1995 May 5;270(18):10432-8
references.pmid: 7737977
references.citation: Plant Cell. 2005 Mar;17(3):691-704
references.pmid: 15705957
references.citation: Int Rev Cytol. 2002;218:1-35
references.pmid: 12199516
references.citation: EMBO J. 1991 Sep;10(9):2635-44
references.pmid: 1868837
references.citation: Plant J. 2012 Jul;71(2):314-26
references.pmid: 22409594
references.citation: Plant Cell. 2004 Feb;16(2):533-43
references.pmid: 14742873
references.citation: Trends Plant Sci. 2007 Sep;12(9):419-26
references.pmid: 17698401
references.citation: Nucleic Acids Res. 2002 Feb 1;30(3):623-35
references.pmid: 11809873
references.citation: Curr Opin Plant Biol. 2008 Oct;11(5):486-94
references.pmid: 18653378
references.citation: Plant Physiol. 1985 Dec;79(4):1072-6
references.pmid: 16664532
references.citation: Plant Physiol. 2005 Sep;139(1):509-18
references.pmid: 16055682
references.citation: Annu Rev Cell Dev Biol. 1995;11:155-88
references.pmid: 8689555
references.citation: Plant Cell Physiol. 2009 May;50(5):970-5
references.pmid: 19324928
references.citation: Mol Gen Genet. 2000 Feb;263(1):12-21
references.pmid: 10732669
references.citation: Development. 1998 Aug;125(15):2935-42
references.pmid: 9655815
references.citation: Plant Cell Rep. 2010 Oct;29(10):1147-56
references.pmid: 20676891
references.citation: Plant J. 2009 Jul;59(2):266-77
references.pmid: 19392702
references.citation: EMBO J. 1993 Sep;12(9):3507-17
references.pmid: 8253077
references.citation: New Phytol. 2011 Jun;190(4):823-837
references.pmid: 21517872
references.citation: Plant Cell Physiol. 2005 Oct;46(10):1646-56
references.pmid: 16081527
references.citation: Nat Biotechnol. 1999 Mar;17(3):287-91
references.pmid: 10096298
references.citation: Plant Cell Physiol. 2003 Dec;44(12):1350-8
references.pmid: 14701930
references.citation: Plant J. 2002 Dec;32(6):1011-22
references.pmid: 12492842
references.citation: Plant Physiol. 1998 Mar;116(3):1097-110
references.pmid: 9501143
references.citation: Plant J. 2001 Feb;25(4):389-98
references.pmid: 11260495
references.citation: PLoS One. 2011;6(12):e28488
references.pmid: 22164299
references.citation: Mol Biol Evol. 2009 Dec;26(12):2775-94
references.pmid: 19734295
references.citation: PLoS One. 2012;7(11):e49836
references.pmid: 23185451