title: Preliminary studies on cryopreservation of snakehead (Channa striata) embryos.
abstract: This paper reports the findings of the ongoing studies on cryopreservation of the snakehead, Channa striata embryos. The specific objective of this study was to collect data on the sensitivity of C. striata embryo hatching rate to low temperatures at two different developmental stages in the presence of four different cryoprotectants. Embryos at morula and heartbeat stages were selected and incubated in 1M dimethyl sulfoxide (Me2SO), 1M ethylene glycol (EG), 1M methanol (MeOH) and 0.1M sucrose solutions at different temperatures for a period of time. Embryos were kept at 24 °C (control), 15 °C, 4 °C and -2 °C for 5 min, 1h and 3h. Following these treatments, the embryos were then transferred into a 24 °C water bath until hatch to evaluate the hatching rate. The results showed that there was a significant decrease of hatching rate in both developmental stages following exposure to 4 °C and -2 °C at 1h and 3h exposure in each treatment. Heartbeat stage was more tolerant against chilling at -2 °C for 3h exposure in Me2SO followed by MeOH, sucrose and EG. Further studies will be conducted to find the best method to preserve embryos for long term storage.
authors.affiliation: School of Biological Sciences, Universiti Sains Malaysia, 11800 USM, Pulau Pinang, Malaysia. Electronic address: sharifuddin85@gmail.com.
authors.forename: M
authors.initials: M
authors.lastname: Mohd Sharifuddin
authors.affiliation: School of Biological Sciences, Universiti Sains Malaysia, 11800 USM, Pulau Pinang, Malaysia; Centre for Marine and Coastal Studies, Universiti Sains Malaysia, 11800 USM, Pulau Pinang, Malaysia. Electronic address: sazizah@usm.my.
authors.forename: M N
authors.initials: MN
authors.lastname: Siti Azizah
mesh_terms: D000818:Animals; D003080:Cold Temperature; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004625:Embryo, Nonmammalian; D019855:Ethylene Glycol / Q000494:pharmacology; D005399:Fishes / Q000196:embryology*; D000432:Methanol / Q000494:pharmacology; D009028:Morula / Q000502:physiology*; D009200:Myocardial Contraction / Q000187:drug effects; D013395:Sucrose / Q000494:pharmacology
chemical_list: D003451:Cryoprotective Agents; D013395:Sucrose; D019855:Ethylene Glycol; D000432:Methanol; D004121:Dimethyl Sulfoxide
keywords: Channa striata; Chilling experiment; Cryopreservation; Snakehead