title: HPLC analysis of potentially harmful substances released from dental filing materials available on the EU market.
abstract: BACKGROUND
  Incomplete cross-linking of composite dental materials leads to their susceptibility to degradation in the environment of non-organic and organic solvents, contributing to the release of chemical compounds which are potentially harmful to living organisms.
  
  
  OBJECTIVE
  The aim of the study was an evaluation in in vitro conditions of releasing of potentially toxic substances from six dental composite materials available in EU countries.
  
  
  METHODS
  The following compounds released from the samples stored in water were analyzed: bisphenol A (BPA), triethylene glycol-dimethacrylate (TEGDMA), urethane dimethacrylate (UDMA) and ethylene glycol dimethacrylate (EDGMA). Analysis of the substances was performed with the use of high performance liquid chromatography, after the following incubation periods: 1 hour, 24 hours, 7 days and 30 days.
  
  
  RESULTS
  Among the analyzed substances, after 1 hour of incubation, the highest average concentration was found for TEGDMA - 2045 μg cm(-3) (in Herculite XRV material), after 24 hours - for UDMA 4.402 μg cm(-3) (in Gradia Direct Anterior material) and after 7 and 30 days for TEGDMA: 8.112 and 6.458 μg•cm(-3) respectively (in Charisma material).
  
  
  CONCLUSIONS
  The examined composites used for reconstruction of hard tissues of teeth remain chemically unstable after polymerization, and release potentially harmful substances in conditions of the present study. The dynamics of the releasing of potentially harmful substances is correlated with the period of sample storage in water.
authors.affiliation: Department of Orthodontics, Medical University of Warsaw, Poland.
authors.forename: Konrad
authors.initials: K
authors.lastname: Małkiewicz
authors.affiliation: College of Public Health, Zielona Góra, Poland.
authors.forename: Alfred
authors.initials: A
authors.lastname: Owoc
authors.affiliation: Institute of Chemistry, Siedlce University of Natural Sciences and Humanities, Poland.
authors.forename: Mariusz
authors.initials: M
authors.lastname: Kluska
authors.affiliation: Private Practice, Krakow, Poland.
authors.forename: Kinga
authors.initials: K
authors.lastname: Grzech-Leśniak
authors.affiliation: Department of Drug Technology and Pharmaceutical Biotechnology, Medical University of Warsaw, Poland.
authors.forename: Jadwiga
authors.initials: J
authors.lastname: Turło
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003764:Dental Materials / Q000032:analysis*; D005062:European Union; D015386:Hazardous Substances / Q000032:analysis; D006801:Humans; D013056:Spectrophotometry, Ultraviolet
chemical_list: D003764:Dental Materials; D015386:Hazardous Substances