title: Twenty years of qPCR: a mature technology?
abstract: Quantitative PCR is the "gold standard" technology to quantify nucleic acids and, since the first report describing real-time PCR detection in 1993, its use has been grown exponentially. More recent technological advancements have extended the field of applications ranging from high-resolution melting detection to digital PCR. Nowadays, it is a very accessible technique, but some pitfalls should be overcome in order to achieve robust and reliable analysis.
authors.affiliation: Laboratorio U.O.C. Neurochirurgia, Istituto Giannina Gaslini, Genova, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Raso
authors.forename: Roberto
authors.initials: R
authors.lastname: Biassoni
mesh_terms: D049673:History, 20th Century; D049674:History, 21st Century; D016133:Polymerase Chain Reaction / Q000266:history / Q000379:methods*