title: IL6-174 G/C gene polymorphism and its relation to serum IL6 in Egyptian children with community-acquired pneumonia.
abstract: Cytokines are involved in the pathogenesis of community acquired pneumonia (CAP). The aim of this study is to investigate the association of IL6-174 G/C gene polymorphism with CAP in Egyptian children, to assess its effect on CAP outcome and to determine its effect on the serum IL6 levels in these children. IL6-174 G/C gene polymorphism was genotyped in 210 Egyptian children (100 patients with CAP and 110 healthy controls) using PCR-RFLP, while the serum IL6 levels were measured by ELISA method. We found a significant association between the GG genotype, G allele of IL6-174 G/C SNP and susceptibility to CAP (P=0.02, 0.01 respectively). However, GG genotype and G allele were protective against severe sepsis (p=0.004), acute respiratory failure (p<0.001) and hospital mortality (p<0.001). Serum IL6 levels were significantly increased in these children while there was no relation between GG genotype and serum IL6. In conclusion, IL6-174 G/C gene polymorphism may contribute to susceptibility to CAP in Egyptian children.
authors.affiliation: Medical Biochemistry Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt. Electronic address: haidy_menna@hotmail.com.
authors.forename: Haidy E
authors.initials: HE
authors.lastname: Zidan
authors.affiliation: Pediatrics Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
authors.forename: Rabab M
authors.initials: RM
authors.lastname: Elbehedy
authors.affiliation: Pediatrics Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
authors.forename: Seham F
authors.initials: SF
authors.lastname: Azab
mesh_terms: D000293:Adolescent; D000483:Alleles; D002648:Child; D002675:Child, Preschool; D017714:Community-Acquired Infections / Q000097:blood / Q000235:genetics*; D004534:Egypt; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006801:Humans; D007223:Infant; D015850:Interleukin-6 / Q000097:blood / Q000235:genetics*; D016015:Logistic Models; D008297:Male; D018410:Pneumonia, Bacterial / Q000097:blood / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D011446:Prospective Studies; D012307:Risk Factors
chemical_list: D015850:Interleukin-6
keywords: Community acquired pneumonia; Genetic polymorphisms; Interleukin-6; Pediatrics