title: Determination of urinary aromatic amines in smokers and nonsmokers using a MIPs-SPE coupled with LC-MS/MS method.
abstract: Urinary aromatic amines (AAs) could be used as biomarkers for human exposure to AAs in cigarette smoke. A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the determination of urinary AAs (i.e. 1-naphthylamine (1-NA), 2-naphthylamine (2-NA), 3-aminobiphenyl (3-ABP) and 4-aminobiphenyl (4-ABP)) in smokers and nonsmokers. A molecularly imprinted polymers (MIPs) solid phase extraction (SPE) cartridge was applied to purify urine samples and no derivatization reaction was involved. Each analytes used respective stable isotope internal standards, which could well compensate matrix effect. Lower limit of detections (LODs) for four AAs were obtained and in the range of 1.5-5ngL(-1). Recovery ranged from 87.7±4.5% to 111.3±6.4% and precision were less than 9.9%. The method was applied to analyze urine samples of 40 smokers and 10 nonsmokers. The 24h urinary excretion amounts of total AAs were higher for smokers compared with nonsmokers. What's more, 1-NA, 3-ABP and 4-ABP excretion amounts showed significant differences (p<0.05) between smokers and nonsmokers.
authors.affiliation: Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, China. Electronic address: yujingjing307@163.com.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Yu
authors.affiliation: Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Wang
authors.affiliation: Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, China.
authors.forename: Ge
authors.initials: G
authors.lastname: Zhao
authors.affiliation: Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Wang
authors.affiliation: Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, China.
authors.forename: Li
authors.initials: L
authors.lastname: Ding
authors.affiliation: Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, China.
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, China.
authors.forename: Jianping
authors.initials: J
authors.lastname: Xie
authors.affiliation: Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, China. Electronic address: xiefuwei@sina.com.
authors.forename: Fuwei
authors.initials: F
authors.lastname: Xie
mesh_terms: D015057:1-Naphthylamine / Q000032:analysis*; D015081:2-Naphthylamine / Q000032:analysis*; D000611:Aminobiphenyl Compounds / Q000652:urine*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D057230:Limit of Detection; D054802:Molecular Imprinting / Q000379:methods; D011108:Polymers / Q000737:chemistry; D012907:Smoking / Q000652:urine*; D052616:Solid Phase Extraction / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods; D016482:Urinalysis / Q000379:methods
chemical_list: D000611:Aminobiphenyl Compounds; D011108:Polymers; C006757:4-biphenylamine; D015057:1-Naphthylamine; D015081:2-Naphthylamine; C049671:3-aminobiphenyl
keywords: Aromatic amines; Biomarker; LC–MS/MS; MIPs; Urine