title: Comparison of extraction methods for the identification and quantification of polyphenols in virgin olive oil by ultra-HPLC-QToF mass spectrometry.
abstract: In this work, liquid-liquid extraction (LLE) and solid phase extraction (SPE), of polyphenols from a VOO sample were optimised by a Plackett-Burman experimental design; then the two extraction techniques capabilities were compared. By using HPLC-DAD, the extraction ability of SPE with the diol phase and LLE were similar. The two methods were further evaluated with ultra HPLC-ESI QToF in negative ion mode by recoveries of standards and matched comparison of the peak area of 40 identified and 27 unidentified compounds. Conclusions indicate that LLE gives better recoveries for highly polar, non-polar, and some polyphenols suspected to contain a nitrogen atom, while for the others the two methods seem to be equally suitable. The presence of nitrogen-containing polyphenols was confirmed in positive ionisation mode in LLE extract, whereas in the SPE extract they were not present. One of them was tentatively identified as a compound containing tyrosine and methyl-decarboxymetyl-eleanoic acid moieties.
authors.affiliation: Dipartimento di Chimica, Sapienza Università di Roma, Piazzale Aldo Moro 5, 00185 Rome, Italy. Electronic address: annalaura.capriotti@uniroma1.it.
authors.forename: Anna Laura
authors.initials: AL
authors.lastname: Capriotti
authors.affiliation: Dipartimento di Chimica, Sapienza Università di Roma, Piazzale Aldo Moro 5, 00185 Rome, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Cavaliere
authors.affiliation: Dipartimento di Scienze Farmaceutiche e Biomediche, Università di Salerno, Via Ponte Don Melillo, 84084 Fisciano, SA, Italy.
authors.forename: Carlo
authors.initials: C
authors.lastname: Crescenzi
authors.affiliation: Dipartimento di Chimica, Sapienza Università di Roma, Piazzale Aldo Moro 5, 00185 Rome, Italy.
authors.forename: Patrizia
authors.initials: P
authors.lastname: Foglia
authors.affiliation: Dipartimento di Chimica, Sapienza Università di Roma, Piazzale Aldo Moro 5, 00185 Rome, Italy.
authors.forename: Riccardo
authors.initials: R
authors.lastname: Nescatelli
authors.affiliation: Dipartimento di Chimica, Sapienza Università di Roma, Piazzale Aldo Moro 5, 00185 Rome, Italy.
authors.forename: Roberto
authors.initials: R
authors.lastname: Samperi
authors.affiliation: Dipartimento di Chimica, Sapienza Università di Roma, Piazzale Aldo Moro 5, 00185 Rome, Italy.
authors.forename: Aldo
authors.initials: A
authors.lastname: Laganà
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D059625:Liquid-Liquid Extraction / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D000069463:Olive Oil; D010938:Plant Oils / Q000737:chemistry*; D059808:Polyphenols / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000379:methods*
chemical_list: D000069463:Olive Oil; D010938:Plant Oils; D059808:Polyphenols
keywords: Experimental design; LC–MS; Liquid–liquid extraction (LLE); Polyphenols; Solid phase extraction (SPE); Virgin olive oil