title: Therapeutic Effects of Myeloid Cell Leukemia-1 siRNA on Human Acute Myeloid Leukemia Cells.
abstract: OBJECTIVE
  Up-regulation of Mcl-1, a known anti-apoptotic protein, is associated with the survival and progression of various malignancies including leukemia. The aim of this study was to explore the effect of Mcl-1 small interference RNA (siRNA) on the proliferation and apoptosis of HL-60 acute myeloid leukemia (AML) cells.
  
  
  METHODS
  siRNA transfection was performed using Lipofectamine™2000 reagent. Relative mRNA and protein expressions were quantified by quantitative real-time PCR and Western blotting, respectively. Trypan blue assay was performed to assess tumor cell proliferation after siRNA transfection. The cytotoxic effect of Mcl-1 siRNA on leukemic cells was measured using MTT assay. Apoptosis was detected using ELISA cell death assay.
  
  
  RESULTS
  Mcl-1 siRNA clearly lowered both Mcl-1 mRNA and protein levels in a time-dependent manner, leading to marked inhibition of cell survival and proliferation. Furthermore, Mcl-1 down-regulation significantly enhanced the extent of HL-60 apoptotic cells.
  
  
  CONCLUSIONS
  Our results suggest that the down-regulation of Mcl-1 by siRNA can effectively trigger apoptosis and inhibit the proliferation of leukemic cells. Therefore, Mcl-1 siRNA may be a potent adjuvant in AML therapy.
authors.affiliation: Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. ; Department of Biochemistry, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Hadi
authors.initials: H
authors.lastname: Karami
authors.affiliation: Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Behzad
authors.initials: B
authors.lastname: Baradaran
authors.affiliation: Hematology and Oncology Research Center, Shahid Ghazi Hospital, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Esfahani
authors.affiliation: Faculty of Medicine, University of Liverpool, Liverpool, United Kingdom.
authors.forename: Masoud
authors.initials: M
authors.lastname: Sakhinia
authors.affiliation: Department of Genetics, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran. ; Tuberculosis and Lung Disease Reseach Center, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Ebrahim
authors.initials: E
authors.lastname: Sakhinia
keywords: Apoptosis; HL-60; Leukemia; Mcl-1; Proliferation; siRNA
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