title: Characterization and antitumor activities of the water-soluble polysaccharide from Rhizoma Arisaematis.
abstract: A water soluble polysaccharide (RAP-W1) was purified from Rhizoma Arisaematis and its antitumor activity was evaluated in BALB/c mice bearing human breast cancer MCF-7. RAP-W1 had the following physicochemical properties: total carbohydrate content (95.9%); no protein; molecular weight (≈57 kDA); monosaccharides composition (rhamnose:fucose:arabinose:mannose:galactose:glucose=0.4:0.5:0.3:0.6:0.9:5.3). After 14 days' treatment to tumor-bearing mice, RAP-W1 could significantly inhibit the growth of tumor transplanted in mice and increase the body weight and the spleen index. Moreover, RAP-W1 could significantly stimulate Con A- or LPS-induced splenocyte proliferation in tumor-bearing mice, as well as enhance the CTL activity. The level of Th1 cytokines (INF-γ and IL-2) in the serum of tumor-bearing mice was increased by RAP-W1 treatment, whereas the Th2 cytokine (IL-10) secretion displayed a dramatic reduction. All the data implied that RAP-W1 can activate T cells by up-regulating Th1/Th2 cytokine ratio, which might partially cause the inhibition of tumor growth.
authors.affiliation: Department of Radiology, Xijing Hospital, the Fourth Military Medical University, Xi'an 710032, PR China.
authors.forename: Geng
authors.initials: G
authors.lastname: Chen
authors.affiliation: Department of Periodontology and Oral Medicine, School of Stomatology, the Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Xu
authors.affiliation: Department of Radiation Medicine, School of Military Preventive Medicine, the Fourth Military Medical University, Xi'an 710032, PR China.
authors.forename: Xia
authors.initials: X
authors.lastname: Miao
authors.affiliation: Department of Radiology, Xijing Hospital, the Fourth Military Medical University, Xi'an 710032, PR China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Huan
authors.affiliation: The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, PR China.
authors.forename: Xiaogang
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Obstetrics and Gynecology, TangDu Hospital, the Fourth Military Medical University, Xi'an 710038, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Ju
authors.affiliation: Department of Anesthesia, the Second Affiliated Hospital, School of Medicine, Xi'an Jiaotong University, Xi'an 710004, PR China.
authors.forename: Xinsheng
authors.initials: X
authors.lastname: Han
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D001940:Breast / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D001943:Breast Neoplasms / Q000097:blood / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D049109:Cell Proliferation / Q000187:drug effects; D016207:Cytokines / Q000097:blood / Q000276:immunology; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D005260:Female; D006801:Humans; D007155:Immunologic Factors / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D056747:Immunomodulation / Q000187:drug effects; D061986:MCF-7 Cells; D051379:Mice; D008807:Mice, Inbred BALB C; D030021:Pinellia / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D027343:Rhizome / Q000737:chemistry*; D012995:Solubility; D014867:Water / Q000737:chemistry
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D016207:Cytokines; D004365:Drugs, Chinese Herbal; D007155:Immunologic Factors; D011134:Polysaccharides; D014867:Water