title: HPLC-DPPH screening method for evaluation of antioxidant compounds extracted from Semen Oroxyli.
abstract: Semen Oroxyli, derived from the seed of Oroxylum indicum L., is a commonly used Traditional Chinese Medicine with beneficial effects against several respiratory disorders. Antioxidative flavonoids may be partly responsible for its medicinal functions. The aim of this study was to rapidly determine the antioxidants in Semen Oroxyli based on a HPLC-DPPH method. Four major flavonoids, baicalein-7-O-gentiobioside, baicalein-7-O-glucoside, baicalein, and baicalin, were identified as the active components against DPPH free radicals, which is in accord with the results of our former traditional activity-guided phytochemical study. The oxidative products of the four antioxidant flavonoids were studied in the DPPH spiking HPLC assay, it was suggested that the three active flavonoid glycosides were converted into 5,6-dihydroxy-7-methoxyflavone, which implied that an additional hydroxyl at C-6 in 5,7-dihydroxyflavones plays an important role in the DPPH assay.
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China. yanry2009@163.com.
authors.forename: Renyi
authors.initials: R
authors.lastname: Yan
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China. caoyangyanghebei@163.com.
authors.forename: Yangyang
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China. ybinmm@hotmail.com.
authors.forename: Bin
authors.initials: B
authors.lastname: Yang
mesh_terms: D029402:Bignoniaceae / Q000737:chemistry*; D001713:Biphenyl Compounds / Q000037:antagonists & inhibitors; D002851:Chromatography, High Pressure Liquid; D044950:Flavanones / Q000737:chemistry* / Q000302:isolation & purification; D047309:Flavones / Q000737:chemistry* / Q000302:isolation & purification; D005419:Flavonoids / Q000737:chemistry* / Q000302:isolation & purification; D016166:Free Radical Scavengers / Q000737:chemistry* / Q000302:isolation & purification; D005960:Glucosides / Q000737:chemistry* / Q000302:isolation & purification; D010851:Picrates / Q000037:antagonists & inhibitors; D010936:Plant Extracts / Q000737:chemistry; D012639:Seeds / Q000737:chemistry; D013329:Structure-Activity Relationship
chemical_list: D001713:Biphenyl Compounds; D044950:Flavanones; D047309:Flavones; D005419:Flavonoids; D016166:Free Radical Scavengers; D005960:Glucosides; D010851:Picrates; D010936:Plant Extracts; C550991:baicalein-7-O-glucoside; C038044:baicalin; C006680:baicalein; C004931:1,1-diphenyl-2-picrylhydrazyl
references.citation: Bioorg Med Chem Lett. 2010 Jan 1;20(1):117-20
references.pmid: 19948405
references.citation: J Chromatogr A. 2003 Feb 21;988(1):95-105
references.pmid: 12647824
references.citation: Pharmacol Ther. 2006 Aug;111(2):476-94
references.pmid: 16458359
references.citation: Anal Chim Acta. 2010 Aug 18;675(1):76-82
references.pmid: 20708120
references.citation: J Chromatogr Sci. 2013 May-Jun;51(5):477-84
references.pmid: 23076784
references.citation: J Pharm Pharmacol. 2013 Feb;65(2):271-9
references.pmid: 23278695
references.citation: Anal Bioanal Chem. 2012 Mar;402(9):2965-76
references.pmid: 22297857
references.citation: Yao Xue Xue Bao. 1964 Nov;11:762-7
references.pmid: 14235244
references.citation: Anal Chem. 2000 May 15;72(10):2323-8
references.pmid: 10845381
references.citation: Fitoterapia. 2011 Sep;82(6):841-8
references.pmid: 21596112
references.citation: J Chromatogr A. 2001 Mar 30;912(1):73-82
references.pmid: 11307989
references.citation: Biochem Pharmacol. 2006 Nov 30;72(11):1439-52
references.pmid: 16920072
references.citation: Planta Med. 1977 May;31(3):257-8
references.pmid: 866485
references.citation: Curr Med Chem. 2007;14(7):827-45
references.pmid: 17346166
references.citation: Biomed Chromatogr. 2012 Apr;26(4):449-57
references.pmid: 21882208