title: Positive correlation between the efficiency of induced pluripotent stem cells and the development rate of nuclear transfer embryos when the same porcine embryonic fibroblast lines are used as donor cells.
abstract: Induced pluripotent stem cells (iPSCs) and nuclear transfer (NT) are two of the primary routes to reprogram differentiated cells back to the pluripotent state. However, it is still unknown whether there is any correlation between the reprogramming efficiency of iPSCs and NT if the same donor cells are employed. In this study, six porcine embryonic fibroblast (PEF) lines from Landrace (L1, L6, L9) or Congjiang local pigs (C4, C5, C6) were used for iPSC induction and NT. Furthermore, the resultant iPSCs from four PEF lines (L1, L6, C4, and C5) were used for NT (iPSC-NT), and the expression of exogenous genes was detected in iPSC-NT embryos by real-time PCR. The results showed that the efficiency of iPSC lines established from different PEF lines were significantly different. When the same PEF lines were used as donor cells for NT, the blastocysts rates were also different among different PEF lines and positively related with iPSCs induction efficiency. When the iPSCs were used as donor cells for NT, compared with the source PEFs, the blastocysts rates were significantly decreased. Real-time PCR results indicated that exogenous genes (Oct4, c-Myc) continued to be expressed in iPSC-NT embryos. In summary, our results demonstrate that there was a positive correlation between iPSCs and NT reprogramming efficiency, although the mechanism of these two routes is different. This may provide a new method to select the appropriate donor cells for inducing iPSCs.
authors.affiliation: 1 College of Life Science, Northeast Agricultural University of China , Harbin, 150030, China .
authors.forename: Bingteng
authors.initials: B
authors.lastname: Xie
authors.forename: Jianyu
authors.initials: J
authors.lastname: Wang
authors.forename: Shichao
authors.initials: S
authors.lastname: Liu
authors.forename: Jiaqiang
authors.initials: J
authors.lastname: Wang
authors.forename: Binghua
authors.initials: B
authors.lastname: Xue
authors.forename: Jingyu
authors.initials: J
authors.lastname: Li
authors.forename: Renyue
authors.initials: R
authors.lastname: Wei
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Zhao
authors.forename: Zhonghua
authors.initials: Z
authors.lastname: Liu
mesh_terms: D000818:Animals; D002467:Cell Nucleus; D004622:Embryo, Mammalian; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D051379:Mice; D053652:Nuclear Transfer Techniques; D060888:Real-Time Polymerase Chain Reaction; D013552:Swine
references.citation: Biol Reprod. 2003 Oct;69(4):1394-400
references.pmid: 12801984
references.citation: Cell. 2012 Dec 21;151(7):1617-32
references.pmid: 23260147
references.citation: Nat Genet. 2009 Sep;41(9):968-76
references.pmid: 19668214
references.citation: Cloning Stem Cells. 2002;4(1):47-55
references.pmid: 12006156
references.citation: PLoS One. 2013 Aug 05;8(8):e70573
references.pmid: 23940595
references.citation: Cell Res. 2013 Jan;23(1):162-6
references.pmid: 23247628
references.citation: Stem Cells. 2010 Apr;28(4):713-20
references.pmid: 20201064
references.citation: J Biol Chem. 2013 Apr 5;288(14):9767-9778
references.pmid: 23439651
references.citation: Epigenetics. 2007 Sep;2(3):179-86
references.pmid: 17965590
references.citation: Cell Reprogram. 2011 Apr;13(2):93-8
references.pmid: 21254851
references.citation: Mol Reprod Dev. 2007 May;74(5):646-54
references.pmid: 17039535
references.citation: Nature. 2009 Sep 3;461(7260):86-90
references.pmid: 19672241
references.citation: Stem Cell Rev Rep. 2013 Oct;9(5):700-8
references.pmid: 23625189
references.citation: Dev Biol. 1962 Apr;4:256-73
references.pmid: 13903027
references.citation: Science. 2001 Apr 27;292(5517):740-3
references.pmid: 11326103
references.citation: Nature. 2007 Jul 19;448(7151):313-7
references.pmid: 17554338
references.citation: Stem Cells Dev. 2012 Nov 1;21(16):3001-9
references.pmid: 22657835
references.citation: PLoS One. 2013 May 21;8(5):e64496
references.pmid: 23704989
references.citation: Anim Reprod Sci. 2012 Sep;134(1-2):45-55
references.pmid: 22921722
references.citation: PLoS One. 2011;6(10):e26406
references.pmid: 22028872
references.citation: Nat Biotechnol. 2008 Jan;26(1):101-6
references.pmid: 18059259
references.citation: Yi Chuan. 2011 Jul;33(7):749-56
references.pmid: 22049689
references.citation: Theriogenology. 2008 Sep 1;70(4):622-30
references.pmid: 18556056
references.citation: Gastroenterology. 2012 Apr;142(4):907-17
references.pmid: 22245845
references.citation: Mol Reprod Dev. 2010 Dec;77(12):1001-15
references.pmid: 20931660
references.citation: Nat Biotechnol. 2008 Nov;26(11):1269-75
references.pmid: 18849973
references.citation: Nature. 2009 Aug 27;460(7259):1132-5
references.pmid: 19668191
references.citation: Biochem Biophys Res Commun. 2006 Feb 3;340(1):183-9
references.pmid: 16356478
references.citation: Development. 2009 Feb;136(4):509-23
references.pmid: 19168672
references.citation: Nature. 1997 Feb 27;385(6619):810-3
references.pmid: 9039911
references.citation: Proc Natl Acad Sci U S A. 2011 Jun 14;108(24):9875-80
references.pmid: 21628574
references.citation: Cell Res. 2010 Jul;20(7):850-3
references.pmid: 20548335
references.citation: PLoS One. 2012;7(4):e28203
references.pmid: 22529890
references.citation: J Reprod Dev. 2005 Feb;51(1):123-31
references.pmid: 15750304
references.citation: Mol Reprod Dev. 2001 Apr;58(4):376-83
references.pmid: 11241773
references.citation: Cell. 2007 Nov 30;131(5):861-72
references.pmid: 18035408
references.citation: J Reprod Fertil. 1999 Mar;115(2):325-31
references.pmid: 10434938
references.citation: Nature. 2008 Jul 3;454(7200):49-55
references.pmid: 18509334