title: Microencapsulation ofLactobacillus acidophilusNCIMB 701748 in matrices containing soluble fibre by spray drying: Technological characterization, storage stability and survival afterin vitrodigestion.
abstract: We evaluated sodium alginate, chitosan and hydroxypropyl methylcellulose (HPMC) as co-encapsulants for spray driedLactobacillus acidophilusNCIMB 701748 by assessing their impact on cell viability and physicochemical properties of the dried powders, viability over 35 days of storage at 25 °C and survival after simulated digestion. Fibres were added to a control carrier medium containing whey protein concentrate, d-glucose and maltodextrin. Sodium alginate and HPMC did not affect cell viability but chitosan reduced viable counts in spray dried powders, as compared to the control. Although chitosan caused large losses of viability during spray-drying, these losses were counteracted by the excellent storage stability compared to control, sodium alginate and HPMC, and the overall effect became positive after the 35-day storage. Chitosan also improved survival rates in simulated GI conditions, however no single fibre could improveL. acidophilusNCIMB 701748 viability in all steps from production through storage and digestion.
authors.affiliation: Division of Food Sciences, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, Leicestershire LE12 5RD, United Kingdom.
authors.forename: Lina
authors.initials: L
authors.lastname: Yonekura
authors.affiliation: Division of Food Sciences, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, Leicestershire LE12 5RD, United Kingdom.
authors.forename: Han
authors.initials: H
authors.lastname: Sun
authors.affiliation: Division of Food Sciences, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, Leicestershire LE12 5RD, United Kingdom.
authors.forename: Christos
authors.initials: C
authors.lastname: Soukoulis
authors.affiliation: Division of Food Sciences, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, Leicestershire LE12 5RD, United Kingdom.
authors.forename: Ian
authors.initials: I
authors.lastname: Fisk
keywords: Chitosan; Gastrointestinal tolerance; HPMC; Heat stress; Probiotics; Sodium alginate
references.citation: J Appl Microbiol. 1999 Jan;86(1):87-92
references.pmid: 10030013
references.citation: Int J Food Microbiol. 2005 Apr 1;99(3):287-96
references.pmid: 15808363
references.citation: Biosci Biotechnol Biochem. 2009 Jan;73(1):196-9
references.pmid: 19129646
references.citation: J Control Release. 2012 Aug 20;162(1):56-67
references.pmid: 22698940
references.citation: Int J Food Microbiol. 2011 Oct 3;149(3):185-93
references.pmid: 21803436
references.citation: Biomacromolecules. 2011 Jul 11;12(7):2834-40
references.pmid: 21574635
references.citation: Antonie Van Leeuwenhoek. 2002 Aug;82(1-4):187-216
references.pmid: 12369188
references.citation: J Appl Microbiol. 2002;93(6):1003-11
references.pmid: 12452956
references.citation: J Pharm Pharmacol. 2012 Jul;64(7):919-30
references.pmid: 22686340
references.citation: Int J Food Microbiol. 2011 Jan 5;144(3):556-60
references.pmid: 21144610
references.citation: J Dairy Sci. 2012 Feb;95(2):558-66
references.pmid: 22281320
references.citation: J Lipid Res. 2007 Feb;48(2):358-65
references.pmid: 17093292
references.citation: Int J Food Microbiol. 2010 Nov 15;144(1):51-63
references.pmid: 20951455
references.citation: Appl Environ Microbiol. 2000 Jun;66(6):2605-12
references.pmid: 10831444
references.citation: J Appl Microbiol. 2004;96(5):1024-39
references.pmid: 15078519
references.citation: BMJ. 1999 Apr 10;318(7189):999-1003
references.pmid: 10195977