title: Presence of growth hormone receptor (GH-R) mRNA and protein in goat ovarian follicles and improvement of in vitro preantral follicle survival and development with GH.
abstract: This study aimed to demonstrate the expression of growth hormone receptor (GH-R) mRNA and protein in goat ovarian follicles in order to investigate the effects of GH on the survival and development of preantral follicles. The ovaries were processed for the isolation of follicles to study GH-R mRNA expression or to localization of GH-R by immunohistochemical analysis. Pieces of ovarian cortex were cultured for 7 days in minimum essential medium(+) (MEM(+)) in the presence or absence of GH at different concentrations (1, 10, 50, 100, and 200 ng/mL). High expression levels of GH-R mRNA were observed in granulosa/theca cells from large antral follicles. However, preantral follicles do not express mRNA for GH-R. Immunohistochemistry demonstrated that the GH-R protein was expressed in the oocytes/granulosa cells of antral follicles, but any protein expression was observed in preantral follicles. The highest (P < 0.05) rate of normal follicles and intermediate follicles was observed after 7 days in MEM(+) plus 10 ng/mL GH (70%). In conclusion, GH-R mRNA and protein are expressed in caprine antral follicles, but not in preantral follicles. Moreover, GH maintains the survival of goat preantral follicles and promotes the development of primordial follicles.
authors.affiliation: Laboratory of Manipulation of Oocytes Enclosed in Preantral Follicles (LAMOFOPA), Veterinary Faculty, State University of Ceará, Fortaleza, Brazil.
authors.forename: F S
authors.initials: FS
authors.lastname: Martins
authors.affiliation: Laboratory of Manipulation of Oocytes Enclosed in Preantral Follicles (LAMOFOPA), Veterinary Faculty, State University of Ceará, Fortaleza, Brazil.
authors.forename: M V A
authors.initials: MV
authors.lastname: Saraiva
authors.affiliation: Potiguar University/Laureate International Universities, Natal, Brazil. Electronic address: dmmvet@hotmail.com.
authors.forename: D M
authors.initials: DM
authors.lastname: Magalhães-Padilha
authors.affiliation: Laboratory of Manipulation of Oocytes Enclosed in Preantral Follicles (LAMOFOPA), Veterinary Faculty, State University of Ceará, Fortaleza, Brazil.
authors.forename: A P
authors.initials: AP
authors.lastname: Almeida
authors.affiliation: Laboratory of Manipulation of Oocytes Enclosed in Preantral Follicles (LAMOFOPA), Veterinary Faculty, State University of Ceará, Fortaleza, Brazil.
authors.forename: J J H
authors.initials: JJ
authors.lastname: Celestino
authors.affiliation: Potiguar University/Laureate International Universities, Natal, Brazil.
authors.forename: R T
authors.initials: RT
authors.lastname: Padilha
authors.affiliation: Biotechnology Nucleus of Sobral (NUBIS)-Federal University of Ceará, Sobral, Brazil.
authors.forename: R M S
authors.initials: RM
authors.lastname: Cunha
authors.affiliation: Biotechnology Nucleus of Sobral (NUBIS)-Federal University of Ceará, Sobral, Brazil.
authors.forename: J R V
authors.initials: JR
authors.lastname: Silva
authors.affiliation: Laboratory of Manipulation of Oocytes Enclosed in Preantral Follicles (LAMOFOPA), Veterinary Faculty, State University of Ceará, Fortaleza, Brazil.
authors.forename: C C
authors.initials: CC
authors.lastname: Campello
authors.affiliation: Laboratory of Manipulation of Oocytes Enclosed in Preantral Follicles (LAMOFOPA), Veterinary Faculty, State University of Ceará, Fortaleza, Brazil.
authors.forename: J R
authors.initials: JR
authors.lastname: Figueiredo
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D005260:Female; D006041:Goats / Q000235:genetics / Q000502:physiology*; D006107:Granulosa Cells / Q000187:drug effects / Q000378:metabolism; D013006:Growth Hormone / Q000494:pharmacology*; D006080:Ovarian Follicle / Q000187:drug effects / Q000254:growth & development*; D012333:RNA, Messenger / Q000378:metabolism; D011986:Receptors, Somatotropin / Q000378:metabolism* / Q000502:physiology; D046509:Tissue Culture Techniques
chemical_list: D012333:RNA, Messenger; D011986:Receptors, Somatotropin; D013006:Growth Hormone
keywords: GH; Goat; Immunohistochemistry; In vitro culture; Preantral follicles activation