title: Analysis of Chromosomal Aberrations and FLT3 gene Mutations in Childhood Acute Myelogenous Leukemia Patients.
abstract: OBJECTIVE
  To identify the well-known common translocations and FLT3 mutations in childhood acute myelogenousleukemia (AML) patients in Turkey.
  
  
  METHODS
  The study included 50 newly diagnosed patients in which t(15;17), t(8;21), and inv(16)chromosomal translocations were identified using real-time PCR and FLT3 gene mutations were identified via direct PCR amplification PCR-RE analysis.
  
  
  RESULTS
  In all, t(15;17) chromosomal aberrations were observed in 4 patients (8.0%), t(8;21) chromosomal aberrationswere observed in 12 patients (24.0%), inv(16) chromosomal aberrations were observed in 3 patients (6.0%), and FLT3-ITD mutations were observed in 2 patients (4.0%); FLT3-D835 point mutation heterozygosity was observed in only 1patient (2.0%) patient.
  
  
  CONCLUSIONS
  Despite of the known literature, a patient with FLT3-ITD and FLT3-D835 double mutation shows a bettersurvival and this might be due to the complementation effect of the t(15;17) translocation. The reportedmutation ratein this article (4%) of FLT3 gene seems to be one of the first results for Turkish population.
authors.affiliation: İstanbul University, Institute of Experimental Medical Research, Department of Genetics, İstanbul, Turkey.
authors.forename: Ender
authors.initials: E
authors.lastname: Coşkunpınar
authors.affiliation: İstanbul University, School of Medicine, Department of Pediatric Hematology-Oncology, İstanbul, Turkey.
authors.forename: Sema
authors.initials: S
authors.lastname: Anak
authors.affiliation: İstanbul University, School of Medicine, Department of Pediatric Hematology-Oncology, İstanbul, Turkey.
authors.forename: Leyla
authors.initials: L
authors.lastname: Ağaoğlu
authors.affiliation: İstanbul University, School of Medicine, Department of Pediatric Hematology-Oncology, İstanbul, Turkey.
authors.forename: Ayşegül
authors.initials: A
authors.lastname: Unüvar
authors.affiliation: İstanbul University, School of Medicine, Department of Pediatric Hematology-Oncology, İstanbul, Turkey.
authors.forename: Omer
authors.initials: O
authors.lastname: Devecioğlu
authors.affiliation: Bakırköy Maternity and Children's Hospital, İstanbul, Turkey.
authors.forename: Gönül
authors.initials: G
authors.lastname: Aydoğan
authors.affiliation: Göztepe Education and Research Hospital, Department of Pediatric Hematology, İstanbul, Turkey.
authors.forename: Cetin
authors.initials: C
authors.lastname: Timur
authors.affiliation: Yüzüncü Yıl University, School of Medicine, Department Of Pediatrics, Van, Turkey.
authors.forename: Ahmet Faik
authors.initials: AF
authors.lastname: Oner
authors.affiliation: Şişli Etfal Education and Research Hospital, Department of Pediatric Hematology, İstanbul, Turkey.
authors.forename: Yıldız
authors.initials: Y
authors.lastname: Yıldırmak
authors.affiliation: İstanbul University, Cerrahpaşa School of Medicine, Department of Pediatric Hematology-Oncology, İstanbul, Turkey.
authors.forename: Tiraje
authors.initials: T
authors.lastname: Celkan
authors.affiliation: İstanbul University, Cerrahpaşa School of Medicine, Department of Pediatric Hematology-Oncology, İstanbul, Turkey.
authors.forename: Inci
authors.initials: I
authors.lastname: Yıldız
authors.affiliation: Kocaeli University, School of Medicine, Department of Children's Health and Diseases, Kocaeli, Turkey.
authors.forename: Nazan
authors.initials: N
authors.lastname: Sarper
authors.affiliation: İstanbul University, Institute of Experimental Medical Research, Department of Genetics, İstanbul, Turkey.
authors.forename: Uğur
authors.initials: U
authors.lastname: Ozbek
keywords: Childhood AML; Chromosomal translocations; D835 mutations; FLT3 gene mutations; ITD
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