title: Supercritical carbon dioxide extraction of carotenoids from pumpkin (Cucurbita spp.): a review.
abstract: Carotenoids are well known for their nutritional properties and health promoting effects representing attractive ingredients to develop innovative functional foods, nutraceutical and pharmaceutical preparations. Pumpkin (Cucurbita spp.) flesh has an intense yellow/orange color owing to the high level of carotenoids, mainly α-carotene, β-carotene, β-cryptoxanthin, lutein and zeaxanthin. There is considerable interest in extracting carotenoids and other bioactives from pumpkin flesh. Extraction procedures able to preserve nutritional and pharmacological properties of carotenoids are essential. Conventional extraction methods, such as organic solvent extraction (CSE), have been used to extract carotenoids from plant material for a long time. In recent years, supercritical carbon dioxide (SC-CO2) extraction has received a great deal of attention because it is a green technology suitable for the extraction of lipophylic molecules and is able to give extracts of high quality and totally free from potentially toxic chemical solvents. Here, we review the results obtained so far on SC-CO2 extraction efficiency and quali-quantitative composition of carotenoids from pumpkin flesh. In particular, we consider the effects of (1) dehydration pre-treatments; (2) extraction parameters (temperature and pressure); the use of water, ethanol and olive oil singularly or in combination as entrainers or pumpkin seeds as co-matrix.
authors.affiliation: Istituto di Scienze delle Produzioni Alimentari-Consiglio Nazionale delle Ricerche (ISPA-CNR), Via Prov. le Lecce-Monteroni, Lecce 73100, Italy. miriana.durante@ispa.cnr.it.
authors.forename: Miriana
authors.initials: M
authors.lastname: Durante
authors.affiliation: Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali (Di.S.Te.B.A.), Università del Salento, Via Prov. le Lecce-Monteroni, Lecce 73100, Italy. marcello.lenucci@unisalento.it.
authors.forename: Marcello Salvatore
authors.initials: MS
authors.lastname: Lenucci
authors.affiliation: Istituto di Scienze delle Produzioni Alimentari-Consiglio Nazionale delle Ricerche (ISPA-CNR), Via Prov. le Lecce-Monteroni, Lecce 73100, Italy. giovanni.mita@ispa.cnr.it.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Mita
mesh_terms: D002245:Carbon Dioxide / Q000737:chemistry*; D002338:Carotenoids / Q000302:isolation & purification*; D025924:Chromatography, Supercritical Fluid; D028464:Cucurbita / Q000378:metabolism*; D011312:Pressure; D012997:Solvents / Q000737:chemistry; D013696:Temperature
chemical_list: D012997:Solvents; D002245:Carbon Dioxide; D002338:Carotenoids
references.citation: J Agric Food Chem. 2000 Nov;48(11):5507-11
references.pmid: 11087510
references.citation: J Agric Food Chem. 1999 Sep;47(9):3558-64
references.pmid: 10552685
references.citation: Compr Rev Food Sci Food Saf. 2002 Apr;1(1):33-44
references.pmid: 33451248
references.citation: Plant Physiol. 1993 Feb;101(2):385-390
references.pmid: 12231693
references.citation: J Food Sci. 2012 Nov;77(11):C1169-73
references.pmid: 23106191
references.citation: J Agric Food Chem. 2002 Jan 2;50(1):221-6
references.pmid: 11754571
references.citation: J Agric Food Chem. 2002 Feb 13;50(4):685-9
references.pmid: 11829628
references.citation: J Chromatogr Sci. 1993 Oct;31(10):422-8
references.pmid: 8245174
references.citation: Free Radic Biol Med. 1994 Jul;17(1):77-82
references.pmid: 7959168
references.citation: J Nutr. 1989 Jan;119(1):112-5
references.pmid: 2643693
references.citation: J Nutr. 1990 Jan;120(1):97-104
references.pmid: 2303916
references.citation: Methods Enzymol. 1992;213:403-20
references.pmid: 1435313
references.citation: Food Chem. 2014 Apr 1;148:314-20
references.pmid: 24262563
references.citation: J Sci Food Agric. 2010 Aug 15;90(10):1709-18
references.pmid: 20564441
references.citation: Free Radic Biol Med. 1991;10(6):427-37
references.pmid: 1894165
references.citation: J Nutr. 1998 Nov;128(11):1933-6
references.pmid: 9808645
references.citation: Int J Mol Sci. 2012;13(4):4233-4254
references.pmid: 22605975
references.citation: Bioresour Technol. 2008 Nov;99(16):7835-41
references.pmid: 18378137
references.citation: J Chromatogr A. 2005 May 6;1073(1-2):371-5
references.pmid: 15909543
references.citation: Plant Foods Hum Nutr. 2006 Jun;61(2):73-80
references.pmid: 16758316
references.citation: J Nutr. 2004 Jan;134(1):257S-261S
references.pmid: 14704330
references.citation: J Agric Food Chem. 2012 Oct 24;60(42):10689-95
references.pmid: 23020127
references.citation: Mol Nutr Food Res. 2009 Sep;53 Suppl 2:S194-218
references.pmid: 19035552