title: cDNA cloning and transcriptional expression profiles of a hexamerin in the oriental fruit fly, Bactrocera dorsalis.
abstract: A Bactrocera dorsalis hexamerin (BdAr) cDNA was cloned (GenBank accession no. KF815528), and its transcriptional expression profiles were determined. The complete 2,530-bp cDNA encodes a 780-amino acid protein with a predicted molecular mass of 94.01 kDa. The proportions of phenylalanine (7.8%), tyrosine (11.2%), and methionine (2.6%) in BdAr as well as all other amino acids are reported. BdAr transcripts were detected in the brain, flight muscle, foregut, Malpighian tubules, and fat body. In the larval stage, BdAr transcripts were expressed in the early third instar and increased in the late third instar. In pupae, the highest expression of BdAr mRNA was present on day 1, then declined and persisted through day 2 to day 8. In adult females, the relative expression of BdAr was significantly higher on day 0 and day 1 compared to day 6 to day 10 while it was highest in newly eclosed adult males. The comparison of the BdAr expression between 8-10-day-old males and females showed a higher level in females. Our phylogenetic analysis results suggest to us that BdAr is similar to Drosophila larval serum protein 1γ.
authors.affiliation: Bachelor Program of Biotechnology, National Chung Hsing University, Taichung, Taiwan.
authors.forename: Meng-Chen
authors.initials: MC
authors.lastname: Tsai
authors.forename: Mei-Er
authors.initials: ME
authors.lastname: Chen
authors.forename: Cheng-Lung
authors.initials: CL
authors.lastname: Tsai
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005216:Fat Body / Q000254:growth & development* / Q000378:metabolism; D005260:Female; D019476:Insect Proteins / Q000235:genetics*; D007814:Larva / Q000254:growth & development / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D011679:Pupa / Q000254:growth & development / Q000378:metabolism; D012737:Sex Factors; D033621:Tephritidae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D019476:Insect Proteins; C041137:storage proteins, Insecta
keywords: Bactrocera dorsalis; fat body; hexamerin; transcriptional expression