title: The relative proportions of different lipid classes and their fatty acid compositions change with culture age in the cariogenic dental pathogen Streptococcus mutans UA159.
abstract: The oral cariogenic bacterial pathogen Streptococcus mutans strain UA159 has become an important research organism strain since its genome was sequenced. However, there is a paucity of information on its lipidome using direct analytical biochemical approaches. We here report on comprehensive analyses of the major lipid classes and their fatty acids in cells grown in batch standing cultures. Using 2-D high-performance thin-layer chromatography lipid class composition changes were detected with culture age. More lipid components were detected in the stationary-phase compared to log-phase cells. The major lipids identified included 1,3-bis(sn-3'-phosphatidyl)-sn-glycerol (phosphatidylglycerol), 1,3-diphosphatidylglycerol (cardiolipin), aminoacyl-phosphatidylglycerol, monoglucosyldiacylglycerol, diglucosyldiacylglycerol, diglucosylmonoacylglycerol and, glycerophosphoryldiglucosyldiacylglycerol. Culture age also affected the fatty acid composition of the total polar lipid fraction. Thus, the major lipid classes detected in log-phase and stationary-phase cells were isolated and their fatty acids were analyzed by gas-liquid chromatography to determine the basis for the fatty acid compositional changes in the total polar lipid fraction. The analyses showed that the relative proportions of these acids changed with culture age within individual lipid classes. Hence fatty acid changes in the total polar lipid fraction reflected changes in both lipid class composition and fatty acid compositions within individual lipid classes.
authors.affiliation: Department of Biological Sciences, University of Cincinnati, Cincinnati, OH, 45221-0006, USA.
authors.forename: Jenny E
authors.initials: JE
authors.lastname: Custer
authors.forename: Bryan D
authors.initials: BD
authors.lastname: Goddard
authors.forename: Stephen F
authors.initials: SF
authors.lastname: Matter
authors.forename: Edna S
authors.initials: ES
authors.lastname: Kaneshiro
mesh_terms: D046508:Culture Techniques; D003731:Dental Caries / Q000382:microbiology; D004075:Diglycerides / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism*; D050356:Lipid Metabolism; D010715:Phosphatidylglycerols / Q000378:metabolism; D013295:Streptococcus mutans / Q000254:growth & development / Q000378:metabolism*
chemical_list: D004075:Diglycerides; D005227:Fatty Acids; D010715:Phosphatidylglycerols
references.citation: J Bacteriol. 2004 Jul;186(13):4152-8
references.pmid: 15205416
references.citation: J Dent Res. 1984 Dec;63(12):1343-7
references.pmid: 6392377
references.citation: Infect Immun. 1980 Feb;27(2):556-62
references.pmid: 7380539
references.citation: J Biol Chem. 2009 Oct 23;284(43):29677-83
references.pmid: 19734140
references.citation: Biochim Biophys Acta. 1988 Nov 25;963(2):359-66
references.pmid: 3196739
references.citation: Biochim Biophys Acta. 1988 Sep 23;962(2):241-7
references.pmid: 2844276
references.citation: FEMS Microbiol Lett. 2000 Aug 1;189(1):89-92
references.pmid: 10913871
references.citation: Can J Biochem Physiol. 1959 Aug;37(8):911-7
references.pmid: 13671378
references.citation: J Bacteriol. 2001 Dec;183(23):6875-84
references.pmid: 11698377
references.citation: J Bacteriol. 2007 Nov;189(22):8139-44
references.pmid: 17827283
references.citation: J Biol Chem. 1986 May 15;261(14):6338-45
references.pmid: 3634775
references.citation: J Clin Microbiol. 1986 Jul;24(1):81-5
references.pmid: 3722368
references.citation: Structure. 2012 Sep 5;20(9):1470-7
references.pmid: 22958643
references.citation: Pol J Microbiol. 2004;53(4):267-72
references.pmid: 15790076
references.citation: J Gen Microbiol. 1976 Jun;94(2):367-72
references.pmid: 950557
references.citation: J Biol Chem. 1957 May;226(1):497-509
references.pmid: 13428781
references.citation: J Bacteriol. 2010 Jun;192(12):3024-32
references.pmid: 20233920
references.citation: Biochim Biophys Acta. 2012 Jul;1831(7):1239-49
references.pmid: 23562838
references.citation: Mol Plant Microbe Interact. 2007 Nov;20(11):1421-30
references.pmid: 17977153
references.citation: Curr Microbiol. 2008 Feb;56(2):110-4
references.pmid: 17960460
references.citation: Appl Environ Microbiol. 2004 Feb;70(2):929-36
references.pmid: 14766573
references.citation: J Chromatogr. 1974 Nov 13;100(1):35-42
references.pmid: 4436414
references.citation: Clin Chim Acta. 1972 Apr;38(1):147-53
references.pmid: 5031779
references.citation: Appl Environ Microbiol. 1977 Feb;33(2):221-6
references.pmid: 848945
references.citation: Infect Immun. 2007 Mar;75(3):1537-9
references.pmid: 17220314
references.citation: Caries Res. 1978;12(1):21-7
references.pmid: 271521
references.citation: Appl Environ Microbiol. 2007 Sep;73(17):5633-8
references.pmid: 17630302
references.citation: FEMS Microbiol Lett. 1995 Mar 1;126(3):257-61
references.pmid: 7729669
references.citation: Proc Natl Acad Sci U S A. 2002 Oct 29;99(22):14434-9
references.pmid: 12397186
references.citation: J Dent Res. 1976 Jan;55:A96-102
references.pmid: 1060642
references.citation: IUBMB Life. 2009 Oct;61(10):940-53
references.pmid: 19787708
references.citation: Appl Environ Microbiol. 2004 Oct;70(10):6181-7
references.pmid: 15466565
references.citation: J Appl Microbiol. 2000 Apr;88(4):555-62
references.pmid: 10792513
references.citation: Microbiol Rev. 1986 Dec;50(4):353-80
references.pmid: 3540569
references.citation: J Lipid Res. 1964 Jan;5:126-7
references.pmid: 14173318
references.citation: J Bacteriol. 2012 Mar;194(6):1401-16
references.pmid: 22267511
references.citation: Arch Oral Biol. 1998 Sep;43(9):735-40
references.pmid: 9783828