title: A Shoot-Specific Hypoxic Response of Arabidopsis Sheds Light on the Role of the Phosphate-Responsive Transcription Factor PHOSPHATE STARVATION RESPONSE1.
abstract: Plant responses to biotic and abiotic stresses are often very specific, but signal transduction pathways can partially or completely overlap. Here, we demonstrate that in Arabidopsis (Arabidopsis thaliana), the transcriptional responses to phosphate starvation and oxygen deficiency stress comprise a set of commonly induced genes. While the phosphate deficiency response is systemic, under oxygen deficiency, most of the commonly induced genes are found only in illuminated shoots. This jointly induced response to the two stresses is under control of the transcription factor PHOSPHATE STARVATION RESPONSE1 (PHR1), but not of the oxygen-sensing N-end rule pathway, and includes genes encoding proteins for the synthesis of galactolipids, which replace phospholipids in plant membranes under phosphate starvation. Despite the induction of galactolipid synthesis genes, total galactolipid content and plant survival are not severely affected by the up-regulation of galactolipid gene expression in illuminated leaves during hypoxia. However, changes in galactolipid molecular species composition point to an adaptation of lipid fluxes through the endoplasmic reticulum and chloroplast pathways during hypoxia. PHR1-mediated signaling of phosphate deprivation was also light dependent. Because a photoreceptor-mediated PHR1 activation was not detectable under hypoxia, our data suggest that a chloroplast-derived retrograde signal, potentially arising from metabolic changes, regulates PHR1 activity under both oxygen and phosphate deficiency.
authors.affiliation: Plant Physiology, University of Bayreuth, 95440 Bayreuth, Germany (M.K., P.G., A.M.);Institute of Molecular Physiology and Biotechnology of Plants, University of Bonn, 53115 Bonn, Germany (H.P., P.D.); andPlant Physiology, Institute of Biology, Humboldt-University of Berlin, 10115 Berlin, Germany (H.S., B.G.).
authors.forename: Maria
authors.initials: M
authors.lastname: Klecker
authors.affiliation: Plant Physiology, University of Bayreuth, 95440 Bayreuth, Germany (M.K., P.G., A.M.);Institute of Molecular Physiology and Biotechnology of Plants, University of Bonn, 53115 Bonn, Germany (H.P., P.D.); andPlant Physiology, Institute of Biology, Humboldt-University of Berlin, 10115 Berlin, Germany (H.S., B.G.).
authors.forename: Philipp
authors.initials: P
authors.lastname: Gasch
authors.affiliation: Plant Physiology, University of Bayreuth, 95440 Bayreuth, Germany (M.K., P.G., A.M.);Institute of Molecular Physiology and Biotechnology of Plants, University of Bonn, 53115 Bonn, Germany (H.P., P.D.); andPlant Physiology, Institute of Biology, Humboldt-University of Berlin, 10115 Berlin, Germany (H.S., B.G.).
authors.forename: Helga
authors.initials: H
authors.lastname: Peisker
authors.affiliation: Plant Physiology, University of Bayreuth, 95440 Bayreuth, Germany (M.K., P.G., A.M.);Institute of Molecular Physiology and Biotechnology of Plants, University of Bonn, 53115 Bonn, Germany (H.P., P.D.); andPlant Physiology, Institute of Biology, Humboldt-University of Berlin, 10115 Berlin, Germany (H.S., B.G.).
authors.forename: Peter
authors.initials: P
authors.lastname: Dörmann
authors.affiliation: Plant Physiology, University of Bayreuth, 95440 Bayreuth, Germany (M.K., P.G., A.M.);Institute of Molecular Physiology and Biotechnology of Plants, University of Bonn, 53115 Bonn, Germany (H.P., P.D.); andPlant Physiology, Institute of Biology, Humboldt-University of Berlin, 10115 Berlin, Germany (H.S., B.G.).
authors.forename: Hagen
authors.initials: H
authors.lastname: Schlicke
authors.affiliation: Plant Physiology, University of Bayreuth, 95440 Bayreuth, Germany (M.K., P.G., A.M.);Institute of Molecular Physiology and Biotechnology of Plants, University of Bonn, 53115 Bonn, Germany (H.P., P.D.); andPlant Physiology, Institute of Biology, Humboldt-University of Berlin, 10115 Berlin, Germany (H.S., B.G.).
authors.forename: Bernhard
authors.initials: B
authors.lastname: Grimm
authors.affiliation: Plant Physiology, University of Bayreuth, 95440 Bayreuth, Germany (M.K., P.G., A.M.);Institute of Molecular Physiology and Biotechnology of Plants, University of Bonn, 53115 Bonn, Germany (H.P., P.D.); andPlant Physiology, Institute of Biology, Humboldt-University of Berlin, 10115 Berlin, Germany (H.S., B.G.) angelika.mustroph@uni-bayreuth.de.
authors.forename: Angelika
authors.initials: A
authors.lastname: Mustroph
references.citation: Plant Physiol. 2011 Aug;156(4):1706-24
references.pmid: 21622811
references.citation: Planta. 1999 Nov;210(1):41-9
references.pmid: 10592031
references.citation: Cell. 1993 Sep 10;74(5):787-99
references.pmid: 7690685
references.citation: Plant Physiol. 2006 Oct;142(2):750-61
references.pmid: 16891548
references.citation: Trends Plant Sci. 2012 Mar;17(3):129-38
references.pmid: 22280796
references.citation: Proc Natl Acad Sci U S A. 2009 Aug 11;106(32):13618-23
references.pmid: 19620738
references.citation: Plant Physiol. 2011 Jul;156(3):1006-15
references.pmid: 21562330
references.citation: Ann Bot. 2005 Sep;96(4):717-26
references.pmid: 16027130
references.citation: Annu Rev Plant Physiol Plant Mol Biol. 1997 Jun;48:223-250
references.pmid: 15012263
references.citation: Planta. 1990 Jun;181(3):399-405
references.pmid: 24196818
references.citation: Annu Rev Plant Biol. 2008;59:313-39
references.pmid: 18444902
references.citation: Proc Natl Acad Sci U S A. 2000 Sep 12;97(19):10649-54
references.pmid: 10973486
references.citation: Curr Opin Biotechnol. 2005 Apr;16(2):123-32
references.pmid: 15831376
references.citation: Physiol Plant. 2010 Jun 1;139(2):129-43
references.pmid: 20113436
references.citation: Science. 1999 Jun 25;284(5423):2181-4
references.pmid: 10381884
references.citation: New Phytol. 2011 Apr;190(2):299-310
references.pmid: 21108648
references.citation: Plant J. 2011 Mar;65(5):690-702
references.pmid: 21208309
references.citation: Proc Natl Acad Sci U S A. 2005 Aug 16;102(33):11934-9
references.pmid: 16085708
references.citation: Curr Opin Plant Biol. 2012 Jun;15(3):261-8
references.pmid: 22226570
references.citation: Planta. 2007 Mar;225(4):907-18
references.pmid: 17033812
references.citation: Plant Cell. 2003 Nov;15(11):2694-706
references.pmid: 14600212
references.citation: Plant Physiol. 2010 Mar;152(3):1391-405
references.pmid: 20071603
references.citation: Proc Natl Acad Sci U S A. 2002 Apr 16;99(8):5732-7
references.pmid: 11960029
references.citation: Cell Mol Life Sci. 2012 Oct;69(19):3269-83
references.pmid: 22903295
references.citation: Nature. 2011 Oct 23;479(7373):419-22
references.pmid: 22020282
references.citation: Clin Chim Acta. 1966 Sep;14(3):361-6
references.pmid: 5970965
references.citation: Plant Physiol. 2009 Apr;149(4):1724-38
references.pmid: 19176720
references.citation: Plant J. 2011 Nov;68(3):560-9
references.pmid: 21749507
references.citation: Philos Trans R Soc Lond B Biol Sci. 2003 Jan 29;358(1429):135-44; discussion 144-5
references.pmid: 12594922
references.citation: Curr Opin Plant Biol. 2011 Oct;14(5):519-29
references.pmid: 21704551
references.citation: Annu Rev Plant Biol. 2006;57:781-803
references.pmid: 16669782
references.citation: Plant Physiol. 1992 Mar;98(3):801-7
references.pmid: 16668751
references.citation: Trends Plant Sci. 2008 May;13(5):221-7
references.pmid: 18439868
references.citation: AoB Plants. 2011;2011:plr030
references.pmid: 22476500
references.citation: Planta. 1985 Aug;165(3):430-8
references.pmid: 24241150
references.citation: Plant Physiol. 1998 Feb 1;116(2):651-8
references.pmid: 9490765
references.citation: Nature. 2003 Jun 19;423(6942):881-5
references.pmid: 12815435
references.citation: New Phytol. 2012 Oct;196(1):13-28
references.pmid: 22897362
references.citation: New Phytol. 2011 Apr;190(2):457-71
references.pmid: 21231933
references.citation: New Phytol. 2002 Sep;155(3):349-361
references.pmid: 33873306
references.citation: Plant Physiol. 2003 Jun;132(2):796-804
references.pmid: 12805609
references.citation: Proc Natl Acad Sci U S A. 2001 Sep 11;98(19):10960-5
references.pmid: 11553816
references.citation: Proc Natl Acad Sci U S A. 2007 Oct 23;104(43):17216-21
references.pmid: 17940034
references.citation: Nat Commun. 2014 Mar 06;5:3425
references.pmid: 24599061
references.citation: Nucleic Acids Res. 2008 Jul 1;36(Web Server issue):W465-9
references.pmid: 18424797
references.citation: New Phytol. 2012 Dec;196(4):1098-1108
references.pmid: 22937909
references.citation: Planta. 2006 Dec;225(1):103-14
references.pmid: 16845530
references.citation: PLoS One. 2011;6(12):e28888
references.pmid: 22194941
references.citation: Planta. 2006 Dec;225(1):139-52
references.pmid: 16802177
references.citation: Plant J. 2009 Jan;57(2):322-31
references.pmid: 18808455
references.citation: Planta. 2004 Jul;219(3):450-8
references.pmid: 15088147
references.citation: Ann Bot. 2009 Jan;103(2):269-80
references.pmid: 18660497
references.citation: Cell Mol Life Sci. 2012 Oct;69(19):3207-24
references.pmid: 22899310
references.citation: Front Plant Sci. 2013 May 21;4:140
references.pmid: 23734154
references.citation: Plant Cell Environ. 2007 Jan;30(1):85-112
references.pmid: 17177879
references.citation: Plant J. 2013 Sep;75(5):726-41
references.pmid: 23672245
references.citation: Planta. 1984 Nov;162(5):441-9
references.pmid: 24253226
references.citation: Plant Physiol. 1995 Apr;107(4):1313-1321
references.pmid: 12228438
references.citation: Plant Physiol. 2005 Mar;137(3):1130-8
references.pmid: 15734908
references.citation: New Phytol. 2006;170(2):213-26
references.pmid: 16608449
references.citation: J Biol Chem. 2002 Aug 30;277(35):31994-2002
references.pmid: 12077151
references.citation: Plant Cell. 2013 Nov;25(11):4285-304
references.pmid: 24249833
references.citation: Plant Physiol. 1996 Sep;112(1):239-247
references.pmid: 12226387
references.citation: Anal Biochem. 1986 Jan;152(1):141-5
references.pmid: 3954036
references.citation: Mol Cell. 2014 Feb 6;53(3):369-79
references.pmid: 24462115
references.citation: Plant J. 2009 Aug;59(4):576-87
references.pmid: 19392705
references.citation: Photosynth Res. 2011 Mar;107(3):223-35
references.pmid: 21290261
references.citation: Ann Bot. 2010 Apr;105(4):513-26
references.pmid: 20181569
references.citation: J Biol Chem. 2001 Aug 24;276(34):31806-12
references.pmid: 11429410
references.citation: PLoS Genet. 2010 Sep 09;6(9):e1001102
references.pmid: 20838596
references.citation: Proc Natl Acad Sci U S A. 1998 Feb 17;95(4):1950-5
references.pmid: 9465123
references.citation: Physiol Plant. 2012 Jan;144(1):35-47
references.pmid: 21910737
references.citation: BMC Biotechnol. 2004 Dec 05;4:31
references.pmid: 15579211
references.citation: Genes Dev. 2001 Aug 15;15(16):2122-33
references.pmid: 11511543
references.citation: Curr Opin Plant Biol. 2012 Feb;15(1):84-91
references.pmid: 22001935
references.citation: Proc Natl Acad Sci U S A. 2009 Mar 17;106(11):4549-54
references.pmid: 19255443
references.citation: Adv Bioinformatics. 2008;2008:420747
references.pmid: 19956698
references.citation: Plant J. 2005 Jan;41(2):257-68
references.pmid: 15634202
references.citation: Plant J. 2008 Dec;56(5):743-55
references.pmid: 18665916
references.citation: Proc Natl Acad Sci U S A. 2009 Nov 3;106(44):18843-8
references.pmid: 19843695
references.citation: Plant J. 2008 Oct;56(1):28-39
references.pmid: 18564386
references.citation: Plant J. 2006 Jun;46(5):890-900
references.pmid: 16709202
references.citation: Plant Signal Behav. 2011 Jul;6(7):942-51
references.pmid: 21628997
references.citation: Plant J. 2004 Jan;37(2):251-68
references.pmid: 14690509
references.citation: BMC Plant Biol. 2012 May 03;12:62
references.pmid: 22553952
references.citation: Plant Cell. 1995 Nov;7(11):1801-10
references.pmid: 8535135
references.citation: Plant Physiol. 1999 Jan;119(1):57-64
references.pmid: 9880346
references.citation: Nature. 2011 Oct 23;479(7373):415-8
references.pmid: 22020279
references.citation: Curr Opin Plant Biol. 2011 Aug;14(4):358-64
references.pmid: 21454121
references.citation: Plant Physiol. 2014 Feb;164(2):570-83
references.pmid: 24368335
references.citation: Proc Natl Acad Sci U S A. 2005 May 24;102(21):7760-5
references.pmid: 15894620
references.citation: Plant Biol (Stuttg). 2014 May;16(3):594-606
references.pmid: 24119171
references.citation: Plant Physiol. 2010 Dec;154(4):1805-18
references.pmid: 20935179
references.citation: Plant J. 2003 Jun;34(6):827-36
references.pmid: 12795702
references.citation: Plant Physiol. 1998 May;117(1):129-39
references.pmid: 9576782
references.citation: Prog Lipid Res. 2011 Jul;50(3):258-66
references.pmid: 21414359
references.citation: Plant Physiol. 2005 Sep;139(1):497-508
references.pmid: 16126859