title: [Expression of tight junction factors in human placental tissues derived from assisted reproductive technology and natural pregnancy].
abstract: OBJECTIVE
  To study the expression of tight junction factors in human placental tissues derived from assisted reproductive technology (ART) and natural pregnancy and its role in placental barrier.
  
  
  METHODS
  Ten placental samples were collected from the women who had undergone ART treatment and 11 placenta were collected from control group. Transmission electron microscope (TEM) examination was utilized to detect the morphology of placental tight junctions. The mRNA of claudin (CLDN) 1, CLDN4, CLDN5, CLDN8, zonula occudens (ZO) 1 was detected by real-time PCR and the protein of CLDN4, CLDN8 and occludin (OCLN) were measured by western blot.
  
  
  RESULTS
  TEM microscopy results showed that placenta samples derived both ART and control placenta had normal microscopic histological features of tight junctions, localized in the apical part of the syncytium and also between the cell-cell contacts of fetal blood vessel endothelial. The expression level of CLDN4 mRNA were 0.87 ± 0.17 in ART group and 1.18 ± 0.30 in control group, respectively. The expression level of CLDN8 mRNA were 3.25 ± 2.32 in ART group and 1.08 ± 0.41 in control group, respectively. The mRNA level of CLDN4 and CLDN8 were significantly differentially expressed in ART derived placenta when compared with control groups. The expression level of CLDN1, CLDN5, OCLN and ZO1 mRNA were 0.49 ± 0.44, 0.80 ± 0.20, 0.92 ± 0.18 in ART group and 1.09 ± 0.82, 1.21 ± 0.78, 0.80 ± 0.27 in control group, respectively, in which there were no significant differences between two groups. Western Blot analysis showed the protein levels of tight junctions CLDN4, CLDN8 and OCLN did not differ between groups.
  
  
  CONCLUSIONS
  Tight junction factors were expressed in human placental tissues. Tight junction derived from ATR platenta might have mild dysfunction.
authors.affiliation: Center of Clinical Reproductive Medicine, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Center of Clinical Reproductive Medicine, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
authors.forename: Nannan
authors.initials: N
authors.lastname: Zhao
authors.affiliation: Center of Clinical Reproductive Medicine, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
authors.forename: Haijun
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Center of Clinical Reproductive Medicine, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
authors.forename: Yugui
authors.initials: Y
authors.lastname: Cui
authors.affiliation: Center of Clinical Reproductive Medicine, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China. Email: jyliu_nj@126.com.
authors.forename: Jiayin
authors.initials: J
authors.lastname: Liu
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D002824:Chorionic Villi / Q000378:metabolism; D057167:Claudins / Q000235:genetics / Q000378:metabolism; D005260:Female; D006801:Humans; D046529:Microscopy, Electron, Transmission; D062793:Occludin / Q000235:genetics / Q000378:metabolism; D010920:Placenta / Q000378:metabolism* / Q000648:ultrastructure; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D027724:Reproductive Techniques, Assisted / Q000009:adverse effects*; D062725:Tight Junction Proteins / Q000235:genetics / Q000378:metabolism*; D019108:Tight Junctions / Q000378:metabolism* / Q000648:ultrastructure; D055815:Young Adult; D062826:Zonula Occludens-1 Protein / Q000235:genetics / Q000378:metabolism
chemical_list: D057167:Claudins; C568985:OCLN protein, human; D062793:Occludin; D012333:RNA, Messenger; C569042:TJP1 protein, human; D062725:Tight Junction Proteins; D062826:Zonula Occludens-1 Protein