title: Genetic diversity of feline morbilliviruses isolated in Japan.
abstract: Feline morbillivirus (FmoPV) is an emerging virus in domestic cats and considered to be associated with tubulointerstitial nephritis. Although FmoPV was first described in China in 2012, there has been no report of the isolation of this virus in other countries. In this report, we describe the isolation and characterization of FmoPV from domestic cats in Japan. By using reverse transcription (RT)-PCR, we found that three of 13 urine samples from cats brought to veterinary hospitals were positive for FmoPV. FmoPV strains SS1 to SS3 were isolated from the RT-PCR-positive urine samples. Crandell-Rees feline kidney (CRFK) cells exposed to FmoPV showed cytopathic effects with syncytia formation, and FmoPV N protein was detected by indirect immunofluorescence assays. In addition, pleomorphic virus particles with apparent glycoprotein envelope spikes were observed by electron microscopy. By sequence analysis of FmoPV H and L genes, we found that FmoPVs showed genetic diversity; however, signatures of positive selection were not identified.
authors.affiliation: Laboratory of Signal Transduction, Department of Cell Biology, Institute for Virus Research, Kyoto University, 53 Shogoin-Kawaharacho, Sakyo-ku, Kyoto 606-8507, Japan.
authors.forename: Shoichi
authors.initials: S
authors.lastname: Sakaguchi
authors.affiliation: Department of Molecular Life Science, Tokai University School of Medicine, 143 Shimokasuya, Isehara, Kanagawa 259-1193, Japan.
authors.forename: So
authors.initials: S
authors.lastname: Nakagawa
authors.affiliation: Laboratory of Signal Transduction, Department of Cell Biology, Institute for Virus Research, Kyoto University, 53 Shogoin-Kawaharacho, Sakyo-ku, Kyoto 606-8507, Japan.
authors.forename: Rokusuke
authors.initials: R
authors.lastname: Yoshikawa
authors.affiliation: Advanced Technology Development Center, Kyoritsu Seiyaku Corporation, 2-9-22 Takamihara, Tsukuba, Ibaraki 300-1252, Japan.
authors.forename: Chieko
authors.initials: C
authors.lastname: Kuwahara
authors.affiliation: Advanced Technology Development Center, Kyoritsu Seiyaku Corporation, 2-9-22 Takamihara, Tsukuba, Ibaraki 300-1252, Japan.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Hagiwara
authors.affiliation: Advanced Technology Development Center, Kyoritsu Seiyaku Corporation, 2-9-22 Takamihara, Tsukuba, Ibaraki 300-1252, Japan.
authors.forename: Ken-Ichi
authors.initials: KI
authors.lastname: Asai
authors.affiliation: Advanced Technology Development Center, Kyoritsu Seiyaku Corporation, 2-9-22 Takamihara, Tsukuba, Ibaraki 300-1252, Japan.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Kawakami
authors.affiliation: National Institute of Animal Health, 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan.
authors.forename: Yu
authors.initials: Y
authors.lastname: Yamamoto
authors.affiliation: Ogawa Pet Clinic, 6-12 Shino-cho, Kameoka, Kyoto 621-0822, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Ogawa
authors.affiliation: Laboratory of Signal Transduction, Department of Cell Biology, Institute for Virus Research, Kyoto University, 53 Shogoin-Kawaharacho, Sakyo-ku, Kyoto 606-8507, Japan.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Miyazawa
mesh_terms: D000818:Animals; D002371:Cat Diseases / Q000821:virology*; D002415:Cats; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D014644:Genetic Variation; D015726:Giant Cells / Q000821:virology; D007564:Japan; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D018103:Morbillivirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D018185:Morbillivirus Infections / Q000662:veterinary* / Q000821:virology; D009395:Nephritis, Interstitial / Q000662:veterinary* / Q000821:virology; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014556:Urine / Q000821:virology; D014771:Virion / Q000648:ultrastructure
chemical_list: D012367:RNA, Viral