title: Spectroscopic investigations, antimicrobial, and cytotoxic activity of green synthesized gold nanoparticles.
abstract: The gold nanoparticles (AuNPs) were synthesized by using naturally available Punica Granatum fruit extract as reducing and stabilizing agent. The biosynthesized AuNPs was characterized by using UV-Vis, fluorescence, high resolution transmission electron microscopy (HRTEM), X-ray diffraction (XRD), Fourier transform infrared (FTIR) and thermogravimetric (TGA) analysis. The surface plasmon resonance (SPR) band at 585nm confirmed the reduction of auric chloride to AuNPs. The crystalline nature of the biosynthesized AuNPs was confirmed from the HRTEM images, XRD and selected area electron diffraction (SAED) pattern. The HRTEM images showed the mixture of triangular and spherical-like AuNPs having size between 5 and 20nm. The weight loss of the AuNPs was measured by TGA as a function of temperature under a controlled atmosphere. The biomolecules are responsible for the reduction of AuCl4(-) ions and the formation of stable AuNPs which was confirmed by FTIR measurement. The synthesized AuNPs showed an excellent antibacterial activity against Candida albicans (ATCC 90028), Aspergillus flavus (ATCC 10124), Staphylococcus aureus (ATCC 25175), Salmonella typhi (ATCC 14028) and Vibrio cholerae (ATCC 14033). The minimum inhibitory concentration (MIC) of AuNPs was recorded against various microorganisms. Further, the synthesized AuNPs shows an excellent cytotoxic result against HeLa cancer cell lines at different concentrations.
authors.affiliation: Department of Inorganic Chemistry, University of Madras, Guindy Maraimalai Campus, Chennai 600 025, India.
authors.forename: S
authors.initials: S
authors.lastname: Lokina
authors.affiliation: Department of Inorganic Chemistry, University of Madras, Guindy Maraimalai Campus, Chennai 600 025, India.
authors.forename: R
authors.initials: R
authors.lastname: Suresh
authors.affiliation: Department of Inorganic Chemistry, University of Madras, Guindy Maraimalai Campus, Chennai 600 025, India.
authors.forename: K
authors.initials: K
authors.lastname: Giribabu
authors.affiliation: Department of Nuclear Physics, University of Madras, Guindy Maraimalai Campus, Chennai 600 025, India.
authors.forename: A
authors.initials: A
authors.lastname: Stephen
authors.affiliation: Sri Ramachandra University, Chennai 600 116, India.
authors.forename: R
authors.initials: R
authors.lastname: Lakshmi Sundaram
authors.affiliation: Department of Inorganic Chemistry, University of Madras, Guindy Maraimalai Campus, Chennai 600 025, India. Electronic address: vnnara@yahoo.co.in.
authors.forename: V
authors.initials: V
authors.lastname: Narayanan
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry* / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology; D006046:Gold / Q000737:chemistry* / Q000494:pharmacology; D055772:Green Chemistry Technology; D006367:HeLa Cells; D006801:Humans; D029561:Lythraceae / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009369:Neoplasms / Q000188:drug therapy; D010084:Oxidation-Reduction; D010936:Plant Extracts / Q000737:chemistry; D013057:Spectrum Analysis
chemical_list: D000890:Anti-Infective Agents; D000970:Antineoplastic Agents; D010936:Plant Extracts; D006046:Gold
keywords: Antimicrobial activity; AuNP; Cytotoxicity; Green synthesis; HeLa cells; Pomegranate