title: Lactobacillus salivarius Ren prevent the early colorectal carcinogenesis in 1, 2-dimethylhydrazine-induced rat model.
abstract: OBJECTIVE
  The objective of this study was to investigate the impact of Lactobacillus salivarius Ren (LS) on modulating colonic micro flora structure and influencing host colonic health in a rat model with colorectal precancerous lesions.
  
  
  RESULTS
  Male F344 rats were injected with 1, 2-dimethylhydrazine (DMH) and treated with LS of two doses (5 × 10(8) and 1 × 10(10) CFU kg(-1) body weight) for 15 weeks. The colonic microflora profiles, luminal metabolites, epithelial proliferation and precancerous lesions [aberrant crypt foci (ACF)] were determined. A distinct segregation of colonic microflora structures was observed in LS-treated group. The abundance of one Prevotella-related strain was increased, and the abundance of one Bacillus-related strain was decreased by LS treatment. These changes were accompanied by increased short-chain fatty acid levels and decreased azoreductase activity. LS treatment also reduced the number of ACF by c. 40% and suppressed epithelial proliferation.
  
  
  CONCLUSIONS
  Lactobacillus salivarius Ren improved the colonic microflora structures and the luminal metabolisms in addition preventing the early colorectal carcinogenesis in DMH-induced rat model.
  
  
  CONCLUSIONS
  Colonic microflora is an important factor in colorectal carcinogenesis. Modulating the structural shifts of microflora may provide a novel option for preventing colorectal carcinogenesis. This study suggested a potential probiotic-based approach to modulate the intestinal microflora in the prevention of colorectal carcinogenesis.
authors.affiliation: Beijing Laboratory for Food Quality and Safety, Key Laboratory of Functional Dairy, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China; Beijing Higher Institution Engineering Research Centre of Animal Product, China Agricultural University, Beijing, China.
authors.forename: J
authors.initials: J
authors.lastname: Zhu
authors.forename: C
authors.initials: C
authors.lastname: Zhu
authors.forename: S
authors.initials: S
authors.lastname: Ge
authors.forename: M
authors.initials: M
authors.lastname: Zhang
authors.forename: L
authors.initials: L
authors.lastname: Jiang
authors.forename: J
authors.initials: J
authors.lastname: Cui
authors.forename: F
authors.initials: F
authors.lastname: Ren
mesh_terms: D019813:1,2-Dimethylhydrazine; D000818:Animals; D000898:Antibiosis; D001407:Bacillus / Q000254:growth & development; D001835:Body Weight / Q000187:drug effects; D063646:Carcinogenesis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D003106:Colon / Q000382:microbiology / Q000473:pathology; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000382:microbiology / Q000473:pathology / Q000517:prevention & control*; D007778:Lactobacillus / Q000502:physiology*; D008297:Male; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D009601:Nitroreductases; D018720:Prevotella / Q000254:growth & development; D019936:Probiotics / Q000494:pharmacology*; D051381:Rats; D011916:Rats, Inbred F344
chemical_list: D009247:NADH, NADPH Oxidoreductases; D009601:Nitroreductases; C021106:azoreductase; D019813:1,2-Dimethylhydrazine
keywords: aberrant crypt foci; colorectal cancer; denaturing gradient gel electrophoresis; intestinal microflora; probiotic; short-chain fatty acids