title: Unravelling the complete genome sequence of Advenella mimigardefordensis strain DPN7T and novel insights in the catabolism of the xenobiotic polythioester precursor 3,3'-dithiodipropionate.
abstract: Advenella mimigardefordensis strain DPN7(T) is a remarkable betaproteobacterium because of its extraordinary ability to use the synthetic disulfide 3,3'-dithiodipropionic acid (DTDP) as the sole carbon source and electron donor for aerobic growth. One application of DTDP is as a precursor substrate for biotechnically synthesized polythioesters (PTEs), which are interesting non-degradable biopolymers applicable for plastics materials. Metabolic engineering for optimization of PTE production requires an understanding of DTDP conversion. The genome of A. mimigardefordensis strain DPN7(T) was sequenced and annotated. The circular chromosome was found to be composed of 4,740,516 bp and 4112 predicted ORFs, whereas the circular plasmid consisted of 23,610 bp and 24 predicted ORFs. The genes participating in DTDP catabolism had been characterized in detail previously, but knowing the complete genome sequence and with support of Tn5: :mob-induced mutants, putatively involved transporter proteins and a transcriptional regulator were also identified. Most probably, DTDP is transported into the cell by a specific tripartite tricarboxylate transport system and is then cleaved by the disulfide reductase LpdA, sulfoxygenated by the 3-mercaptopropionate dioxygenase Mdo, activated by the CoA ligase SucCD and desulfinated by the acyl-CoA dehydrogenase-like desulfinase AcdA. Regulation of this pathway is presumably performed by a transcriptional regulator of the xenobiotic response element family. The excessive sulfate that is inevitably produced is secreted by the cells by a unique sulfate exporter of the CPA (cation : proton antiporter) superfamily.
authors.affiliation: Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität Münster, 48149 Münster, Germany.
authors.forename: Jan Hendrik
authors.initials: JH
authors.lastname: Wübbeler
authors.affiliation: Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität Münster, 48149 Münster, Germany.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Hiessl
authors.affiliation: Department of Genomic and Applied Microbiology and Göttingen Genomics Laboratory, Institut für Mikrobiologie und Genetik, Georg-August-Universität Göttingen, Göttingen, Germany.
authors.forename: Jörg
authors.initials: J
authors.lastname: Schuldes
authors.affiliation: Department of Genomic and Applied Microbiology and Göttingen Genomics Laboratory, Institut für Mikrobiologie und Genetik, Georg-August-Universität Göttingen, Göttingen, Germany.
authors.forename: Andrea
authors.initials: A
authors.lastname: Thürmer
authors.affiliation: Department of Genomic and Applied Microbiology and Göttingen Genomics Laboratory, Institut für Mikrobiologie und Genetik, Georg-August-Universität Göttingen, Göttingen, Germany.
authors.forename: Rolf
authors.initials: R
authors.lastname: Daniel
authors.affiliation: Faculty of Biology, King Abdulaziz University, Jeddah, Saudi Arabia.|Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität Münster, 48149 Münster, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Steinbüchel
mesh_terms: D042401:Alcaligenaceae / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D002876:Chromosomes, Bacterial / Q000235:genetics; D004220:Disulfides / Q000737:chemistry / Q000378:metabolism; D016680:Genome, Bacterial / Q000235:genetics*; D023281:Genomics; D053858:Metabolic Networks and Pathways; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D010802:Phylogeny; D010957:Plasmids / Q000235:genetics; D011108:Polymers / Q000378:metabolism; D011422:Propionates / Q000737:chemistry / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D013431:Sulfates / Q000737:chemistry / Q000378:metabolism; D014050:Toluene / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism; D015262:Xenobiotics / Q000737:chemistry / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D004220:Disulfides; D011108:Polymers; D011422:Propionates; D013431:Sulfates; D015262:Xenobiotics; D014050:Toluene; C004848:dithiol