title: Posttranscriptional regulation of 2,4-diacetylphloroglucinol production by GidA and TrmE in Pseudomonas fluorescens 2P24.
abstract: Pseudomonas fluorescens 2P24 is a soilborne bacterium that synthesizes and excretes multiple antimicrobial metabolites. The polyketide compound 2,4-diacetylphloroglucinol (2,4-DAPG), synthesized by the phlACBD locus, is its major biocontrol determinant. This study investigated two mutants defective in antagonistic activity against Rhizoctonia solani. Deletion of the gidA (PM701) or trmE (PM702) gene from strain 2P24 completely inhibited the production of 2,4-DAPG and its precursors, monoacetylphloroglucinol (MAPG) and phloroglucinol (PG). The transcription of the phlA gene was not affected, but the translation of the phlA and phlD genes was reduced significantly. Two components of the Gac/Rsm pathway, RsmA and RsmE, were found to be regulated by gidA and trmE, whereas the other components, RsmX, RsmY, and RsmZ, were not. The regulation of 2,4-DAPG production by gidA and trmE, however, was independent of the Gac/Rsm pathway. Both the gidA and trmE mutants were unable to produce PG but could convert PG to MAPG and MAPG to 2,4-DAPG. Overexpression of PhlD in the gidA and trmE mutants could restore the production of PG and 2,4-DAPG. Taken together, these findings suggest that GidA and TrmE are positive regulatory elements that influence the biosynthesis of 2,4-DAPG posttranscriptionally.
authors.affiliation: Department of Plant Pathology, China Agricultural University, Beijing, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Plant Pathology, China Agricultural University, Beijing, China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Department of Plant Pathology, China Agricultural University, Beijing, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Department of Plant Pathology, China Agricultural University, Beijing, China.
authors.forename: Xiao-Gang
authors.initials: XG
authors.lastname: Wu
authors.affiliation: Research Institute of Forest Ecology, Environment and Protection, Key Laboratory of State Forestry Administration on Forest Ecology and Protection, Chinese Academy of Forestry, Beijing, China.
authors.forename: Zheng-Guang
authors.initials: ZG
authors.lastname: Ren
authors.affiliation: Department of Plant Pathology, China Agricultural University, Beijing, China Key Laboratory of Plant Pathology, Ministry of Agriculture, Beijing, China zhanglq@cau.edu.cn.
authors.forename: Li-Qun
authors.initials: LQ
authors.lastname: Zhang
mesh_terms: D000898:Antibiosis; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D020558:GTP Phosphohydrolases / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D010696:Phloroglucinol / Q000031:analogs & derivatives* / Q000378:metabolism; D014176:Protein Biosynthesis; D011551:Pseudomonas fluorescens / Q000235:genetics* / Q000378:metabolism*; D012232:Rhizoctonia / Q000187:drug effects; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; C108512:glucose-inhibited division protein A, bacteria; C059817:2,4-diacetylphloroglucinol; D010696:Phloroglucinol; D020558:GTP Phosphohydrolases
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