title: Rat hepatocytes weighted gene co-expression network analysis identifies specific modules and hub genes related to liver regeneration after partial hepatectomy.
abstract: The recovery of liver mass is mainly mediated by proliferation of hepatocytes after 2/3 partial hepatectomy (PH) in rats. Studying the gene expression profiles of hepatocytes after 2/3 PH will be helpful to investigate the molecular mechanisms of liver regeneration (LR). We report here the first application of weighted gene co-expression network analysis (WGCNA) to analyze the biological implications of gene expression changes associated with LR. WGCNA identifies 12 specific gene modules and some hub genes from hepatocytes genome-scale microarray data in rat LR. The results suggest that upregulated MCM5 may promote hepatocytes proliferation during LR; BCL3 may play an important role by activating or inhibiting NF-kB pathway; MAPK9 may play a permissible role in DNA replication by p38 MAPK inactivation in hepatocytes proliferation stage. Thus, WGCNA can provide novel insight into understanding the molecular mechanisms of LR.
authors.affiliation: College of Life Science, Henan Normal University, Xinxiang, Henan, China; Key Laboratory of Cell Differentiation and Regulation, Henan Normal University, Xinxiang, Henan, China; College of Computer and Information Engineering, Henan Normal University, Xinxiang, Henan, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: College of Computer and Information Engineering, Henan Normal University, Xinxiang, Henan, China.
authors.forename: Jiucheng
authors.initials: J
authors.lastname: Xu
authors.affiliation: College of Life Science, Henan Normal University, Xinxiang, Henan, China; Key Laboratory of Cell Differentiation and Regulation, Henan Normal University, Xinxiang, Henan, China.
authors.forename: Yunqing
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Mathematics and Information Science, Henan Normal University, Xinxiang, Henan, China.
authors.forename: Juntao
authors.initials: J
authors.lastname: Li
authors.affiliation: College of Life Science, Henan Normal University, Xinxiang, Henan, China; Key Laboratory of Cell Differentiation and Regulation, Henan Normal University, Xinxiang, Henan, China.
authors.forename: Cuifang
authors.initials: C
authors.lastname: Chang
authors.affiliation: College of Life Science, Henan Normal University, Xinxiang, Henan, China; Key Laboratory of Cell Differentiation and Regulation, Henan Normal University, Xinxiang, Henan, China.
authors.forename: Cunshuan
authors.initials: C
authors.lastname: Xu
mesh_terms: D000818:Animals; D020869:Gene Expression Profiling; D053263:Gene Regulatory Networks; D006498:Hepatectomy; D022781:Hepatocytes / Q000166:cytology / Q000378:metabolism*; D008115:Liver Regeneration / Q000235:genetics*; D058977:Molecular Sequence Annotation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000235:genetics
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