title: Dengue NS1 and prM antibodies increase the sensitivity of acute dengue diagnosis test and differentiate from Japanese encephalitis infection.
abstract: Accurate and early diagnosis of dengue infection is essential for dengue case management. In outbreak conditions, it is essential to include two different tests to diagnose dengue and the choice depends on the number of days after the onset of illness in which the sample is collected. During the laboratory diagnosis of dengue in late acute and convalescent phase by MAC-ELISA, it is necessary to rule out possible cross reactions of closely related flavivirus, such as Japanese encephalitis virus which is commonly co-circulating. In the present investigation, the usefulness of dengue virus NS1 and prM antibodies in diagnosing and differentiating dengue from Japanese encephalitis infection was assessed using samples collected during out-breaks. It was shown here that, detection of antibodies against dengue NS1 and prM proteins increases the sensitivity of dengue diagnosis until 15days. Moreover, detection of antibodies against both proteins was able to differentiate dengue from Japanese encephalitis infection.
authors.affiliation: Department of Biotechnology, Anna University - BIT Campus, Tiruchirappalli-620 024, Tamil Nadu, India. Electronic address: sankar.immuno@yahoo.com.
authors.forename: S
authors.initials: S
authors.lastname: Gowri Sankar
authors.affiliation: Department of Serology, Centre for Research in Medical Entomology (CRME) (WHO-Collaborating Centre for Lymphatic filariasis and Dengue), Indian Council of Medical Research (ICMR), Madurai-625 002, Tamil Nadu, India.
authors.forename: T
authors.initials: T
authors.lastname: Balaji
authors.affiliation: Department of Molecular Biology and Virology, Centre for Research in Medical Entomology (CRME) (WHO-Collaborating Centre for Lymphatic filariasis and Dengue), Indian Council of Medical Research (ICMR), Madurai-625 002, Tamil Nadu, India.
authors.forename: K
authors.initials: K
authors.lastname: Venkatasubramani
authors.affiliation: Department of Serology, Centre for Research in Medical Entomology (CRME) (WHO-Collaborating Centre for Lymphatic filariasis and Dengue), Indian Council of Medical Research (ICMR), Madurai-625 002, Tamil Nadu, India.
authors.forename: V
authors.initials: V
authors.lastname: Thenmozhi
authors.affiliation: Department of Molecular Biology and Virology, Centre for Research in Medical Entomology (CRME) (WHO-Collaborating Centre for Lymphatic filariasis and Dengue), Indian Council of Medical Research (ICMR), Madurai-625 002, Tamil Nadu, India.
authors.forename: K J
authors.initials: KJ
authors.lastname: Dhananjeyan
authors.affiliation: Department of Molecular Biology and Virology, Centre for Research in Medical Entomology (CRME) (WHO-Collaborating Centre for Lymphatic filariasis and Dengue), Indian Council of Medical Research (ICMR), Madurai-625 002, Tamil Nadu, India.
authors.forename: R
authors.initials: R
authors.lastname: Paramasivan
authors.affiliation: Department of Entomology, Centre for Research in Medical Entomology (CRME) (WHO-Collaborating Centre for Lymphatic filariasis and Dengue), Indian Council of Medical Research (ICMR), Madurai-625 002, Tamil Nadu, India.
authors.forename: B K
authors.initials: BK
authors.lastname: Tyagi
authors.affiliation: Department of Biotechnology, Anna University - BIT Campus, Tiruchirappalli-620 024, Tamil Nadu, India. Electronic address: johnvennison36@gmail.com.
authors.forename: S
authors.initials: S
authors.lastname: John Vennison
mesh_terms: D000914:Antibodies, Viral / Q000097:blood; D003429:Cross Reactions; D003715:Dengue / Q000175:diagnosis* / Q000276:immunology; D003716:Dengue Virus / Q000276:immunology*; D003937:Diagnosis, Differential; D003955:Diagnostic Tests, Routine; D018450:Disease Progression; D042241:Early Diagnosis; D004672:Encephalitis, Japanese / Q000175:diagnosis* / Q000276:immunology; D006801:Humans; D007194:India; D012680:Sensitivity and Specificity; D017361:Viral Nonstructural Proteins / Q000276:immunology*; D014764:Viral Proteins / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; C082515:NS1 protein, dengue-1 virus; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins; C084187:prM protein, Dengue virus type 3
keywords: Antigen detection; Cross reactivity; Dengue virus (DENV); Flavi virus detection; Japanese encephalitis virus (JEV); Serological differentiation