title: Comparative QTL analysis of root lesion nematode resistance in barley.
abstract: This study demonstrates for the first time that resistance to different root lesion nematodes ( P. neglectus and P. penetrans ) is controlled by a common QTL. A major resistance QTL ( Rlnnp6H ) has been mapped to chromosome 6H using two independent barley populations. Root lesion nematodes (Pratylenchus spp.) are important pests in cereal production worldwide. We selected two doubled haploid populations of barley (Igri × Franka and Uschi × HHOR 3073) and infected them with Pratylenchus penetrans and Pratylenchus neglectus. Nematode multiplication rates were measured 7 or 10 weeks after infection. In both populations, continuous phenotypic variations for nematode multiplication rates were detected indicating a quantitative inheritance of resistance. In the Igri × Franka population, four P. penetrans resistance QTLs were mapped with 857 molecular markers on four linkage groups (2H, 5H, 6H and 7H). In the Uschi × HHOR 3073 population, eleven resistance QTLs (P. penetrans and P. neglectus) were mapped with 646 molecular markers on linkage groups 1H, 3H, 4H, 5H, 6H and 7H. A major resistance QTL named Rlnnp6H (LOD score 6.42-11.19) with a large phenotypic effect (27.5-36.6 %) for both pests was mapped in both populations to chromosome 6H. Another resistance QTL for both pests was mapped on linkage group 5H (Igri × Franka population). These data provide first evidence for common resistance mechanisms against different root lesion nematode species. The molecular markers are a powerful tool for the selection of resistant barley lines among segregating populations because resistance tests are time consuming and laborious.
authors.affiliation: Plant Breeding Institute, Christian-Albrechts-University of Kiel, Olshausenstrasse 40, 24098, Kiel, Germany.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Galal
authors.forename: Shiveta
authors.initials: S
authors.lastname: Sharma
authors.forename: Salah Fatouh
authors.initials: SF
authors.lastname: Abou-Elwafa
authors.forename: Shailendra
authors.initials: S
authors.lastname: Sharma
authors.forename: Friedrich
authors.initials: F
authors.lastname: Kopisch-Obuch
authors.forename: Eberhard
authors.initials: E
authors.lastname: Laubach
authors.forename: Dragan
authors.initials: D
authors.lastname: Perovic
authors.forename: Frank
authors.initials: F
authors.lastname: Ordon
authors.forename: Christian
authors.initials: C
authors.lastname: Jung
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D060467:Disease Resistance / Q000235:genetics*; D001467:Hordeum / Q000235:genetics* / Q000469:parasitology; D006790:Host-Parasite Interactions / Q000235:genetics*; D009348:Nematoda / Q000502:physiology*; D010935:Plant Diseases; D018517:Plant Roots / Q000235:genetics / Q000469:parasitology; D040641:Quantitative Trait Loci
references.citation: Mol Genet Genomics. 2002 Mar;267(1):16-26
references.pmid: 11919711
references.citation: Theor Appl Genet. 2002 Apr;104(5):874-879
references.pmid: 12582649
references.citation: J Nematol. 1973 Jul;5(3):225-6
references.pmid: 19319337
references.citation: J Nematol. 1989 Oct;21(4):530-9
references.pmid: 19287649
references.citation: Plant Mol Biol. 1998 Dec;38(6):999-1010
references.pmid: 9869406
references.citation: Phytopathology. 1998 Nov;88(11):1141-8
references.pmid: 18944846
references.citation: BMC Genomics. 2006 Aug 12;7:206
references.pmid: 16904008
references.citation: J Nematol. 2005 Dec;37(4):473-6
references.pmid: 19262893
references.citation: J Nematol. 1987 Oct;19(4):424-30
references.pmid: 19290166
references.citation: New Phytol. 2008;180(1):27-44
references.pmid: 18564304
references.citation: J Nematol. 1997 Sep;29(3):306-14
references.pmid: 19274163
references.citation: Plant Mol Biol. 1998 Jan;36(2):219-27
references.pmid: 9484434
references.citation: Nature. 2012 Nov 29;491(7426):711-6
references.pmid: 23075845
references.citation: Genetics. 1994 Nov;138(3):963-71
references.pmid: 7851788
references.citation: Nature. 2012 May 16;486(7402):228-32
references.pmid: 22699612
references.citation: Theor Appl Genet. 2011 May;122(7):1321-30
references.pmid: 21298411
references.citation: Theor Appl Genet. 1991 Dec;83(2):250-6
references.pmid: 24202366
references.citation: Phytopathology. 2000 Nov;90(11):1239-45
references.pmid: 18944426
references.citation: J Nematol. 2000 Dec;32(4S):591-9
references.pmid: 19271014
references.citation: Theor Appl Genet. 2008 Nov;117(8):1261-70
references.pmid: 18712341