title: Isolation, in silico characterization, localization and expression analysis of abiotic stress-responsive rice G-protein β subunit (RGB1).
abstract: Heterotrimeric G-proteins constitute the classical signaling paradigm along with their cognate G-protein coupled receptors (GPCRs) and appropriate downstream effectors. G-protein complex is composed of highly conserved Gα, Gβ, and Gγ subunits. In the present study, we have characterized the cis-regulatory elements of the promoter, signature motifs, transcript profile in response to abiotic stresses, and sub-cellular localization of G-protein β subunit RGB1(I) from Indica rice. The RGB1(I) promoter sequence has various stress-related cis-regulatory elements suggesting its role in abiotic stress signaling. Presence of six WD-40 repeat signature motifs in RGB1(I) suggest its role in exchange of GDP by GTP in Gα subunit and receptor recognition. Presence of multiple N-myristoylation consensus sites in RGB1(I) protein sequence, which is necessary for membrane localization of protein, confirms the association of RGB1(I) in plasma membrane. Extrinsic association of RGB1(I) with plasma membrane seems essential for its role in regulation of signaling pathways and adaptation to high salt stress. We report the sub-cellular localization of RGB1(I) in plasma membrane, cytosol and nucleus. The localization of RGB1(I) in nucleus supports its possible interaction with transcription factors regulating the expression of salt stress responsive genes. The RGB1(I) transcript was upregulated under KCl, cold, dehydration and micronutrient (Mn (2+) and Zn (2+)) stress. However, transcript variation under elevated temperature, ABA, NaCl, and toxic heavy metals (viz. arsenite, arsenate, cadmium and lead) was not encouraging. These evidences indicate an active and significant role of RGB1(I) in the regulation of abiotic stresses in rice and propound its possible exploitation in the development of abiotic stress tolerance in crops.
authors.affiliation: Plant Molecular Biology Group; International Centre for Genetic Engineering and Biotechnology; Aruna Asaf Ali Marg, New Delhi, India.
authors.forename: Dinesh K
authors.initials: DK
authors.lastname: Yadav
authors.affiliation: Plant Molecular Biology Group; International Centre for Genetic Engineering and Biotechnology; Aruna Asaf Ali Marg, New Delhi, India.
authors.forename: Devesh
authors.initials: D
authors.lastname: Shukla
authors.affiliation: Plant Molecular Biology Group; International Centre for Genetic Engineering and Biotechnology; Aruna Asaf Ali Marg, New Delhi, India.
authors.forename: Narendra
authors.initials: N
authors.lastname: Tuteja
mesh_terms: D000595:Amino Acid Sequence; D044387:GTP-Binding Protein beta Subunits / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics / Q000502:physiology*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D054730:Protein Interaction Domains and Motifs; D015398:Signal Transduction; D013312:Stress, Physiological
chemical_list: D044387:GTP-Binding Protein beta Subunits; D010940:Plant Proteins
keywords: Abiotic stress; heterotrimeric G-proteins; metals; rice G-protein beta-subunits; signal transduction; sub-cellular localization
references.citation: Plant Cell Rep. 2007 Sep;26(9):1469-80
references.pmid: 17492287
references.citation: Annu Rev Plant Biol. 2007;58:249-66
references.pmid: 17201690
references.citation: Plant Physiol. 2008 Jun;147(2):636-49
references.pmid: 18441222
references.citation: Mol Biol Evol. 2011 Oct;28(10):2731-9
references.pmid: 21546353
references.citation: Mol Syst Biol. 2011 Sep 27;7:532
references.pmid: 21952135
references.citation: Plant Cell. 2002;14 Suppl:S355-73
references.pmid: 12045288
references.citation: Nucleic Acids Res. 2002 Jan 1;30(1):325-7
references.pmid: 11752327
references.citation: Proc Natl Acad Sci U S A. 1994 Sep 27;91(20):9554-8
references.pmid: 7937804
references.citation: Plant Physiol. 1994 Mar;104(3):1015-25
references.pmid: 7513083
references.citation: New Phytol. 2011 Apr;190(1):35-48
references.pmid: 21175635
references.citation: Curr Opin Plant Biol. 2004 Dec;7(6):719-31
references.pmid: 15491922
references.citation: Cell. 1995 Dec 15;83(6):1047-58
references.pmid: 8521505
references.citation: Proc Natl Acad Sci U S A. 1999 Mar 2;96(5):1936-40
references.pmid: 10051573
references.citation: Genome Biol. 2008;9(7):R120
references.pmid: 18671868
references.citation: EMBO Rep. 2004 Jun;5(6):572-8
references.pmid: 15170476
references.citation: Plant J. 2011 Sep;67(5):907-16
references.pmid: 21585570
references.citation: Biochim Biophys Acta. 2001 Aug 30;1520(2):147-53
references.pmid: 11513956
references.citation: Environ Health Perspect. 1994 Sep;102 Suppl 3:181-9
references.pmid: 7843095
references.citation: Plant J. 2011 Sep;67(5):840-51
references.pmid: 21575088
references.citation: Plant Mol Biol. 2000 Apr;42(6):915-22
references.pmid: 10890537
references.citation: Front Biosci. 2008 May 01;13:3321-33
references.pmid: 18508435
references.citation: Open Biol. 2013 Mar 27;3(3):120186
references.pmid: 23536550
references.citation: Nature. 1996 Jan 25;379(6563):311-9
references.pmid: 8552184
references.citation: Plant J. 2007 Sep;51(6):966-77
references.pmid: 17662034
references.citation: Plant Physiol Biochem. 2013 Feb;63:262-71
references.pmid: 23313793
references.citation: Plant J. 1999 Aug;19(3):237-47
references.pmid: 10476071
references.citation: Plant Cell. 2001 Dec;13(12):2631-41
references.pmid: 11752377
references.citation: Plant Signal Behav. 2011 Aug;6(8):1079-86
references.pmid: 21778827
references.citation: J Cell Biol. 2005 Jun 20;169(6):885-96
references.pmid: 15955845
references.citation: Proc Natl Acad Sci U S A. 1990 May;87(10):3821-5
references.pmid: 2111018
references.citation: Plant Signal Behav. 2012 Jul;7(7):733-40
references.pmid: 22751322
references.citation: J Biol Chem. 2001 Mar 30;276(13):10284-9
references.pmid: 11152459
references.citation: Methods. 2001 Dec;25(4):402-8
references.pmid: 11846609
references.citation: Plant Cell. 2013 Feb;25(2):609-24
references.pmid: 23396830
references.citation: Cell. 1996 Jan 26;84(2):175-8
references.pmid: 8565060
references.citation: Plant Signal Behav. 2011 Feb;6(2):287-92
references.pmid: 21350337
references.citation: Sci STKE. 2000 Dec 19;2000(63):pe1
references.pmid: 11752628
references.citation: Plant J. 1999 May;18(4):455-63
references.pmid: 10406127
references.citation: Plant Signal Behav. 2011 Jun;6(6):780-2
references.pmid: 21543884