title: [Effect of Qufeng Tongluo Recipe on the expressions of connexin 36 protein and gene in rat mesangial cells].
abstract: OBJECTIVE
  To determine the effect of Qufeng Tongluo Recipe (QFTLR) on the expressions of connexin 36 (Cx36) protein and gene in rat mesangial cells (MCs) and the proliferation of the MCs.
  
  
  METHODS
  Serum samples containing Benazepril (Bena) and QFTLR were prepared in line with serum pharmacology methodology. The MCs cultured in vitro were divided into normal control and Lipopolysaccharide (LPS), Bena and QFTLR treated groups. The expressions of Cx36 protein and gene were detected by laser scanning confocal microscope (LSCM), Western blot, immunohistochemical assay and quantitative real time polymerase chain reaction (QRT-PCR) respectively.
  
  
  RESULTS
  Compared with the control, higher level of Cx36 protein expression was found in the MCs than treated with LPS (P < 0.01). Both Bena and QFTLR lowered the level of Cx36 protein expression in the MCs treated with LPS significantly (P < 0.01 or P < 0.05). Similar results were found with the expression of Cx36 mRNA.
  
  
  CONCLUSIONS
  QFTLR inhibits the proliferation of rat MCs, possibly through down-regulating the expressions of Cx36 protein and gene.
authors.forename: Xi-Li
authors.initials: XL
authors.lastname: Wu
authors.forename: Bing-Yu
authors.initials: BY
authors.lastname: Ye
authors.forename: Peng
authors.initials: P
authors.lastname: An
authors.forename: Cheng-Lin
authors.initials: CL
authors.lastname: Qiao
authors.forename: Xing-Min
authors.initials: XM
authors.lastname: Shi
authors.forename: Rong-Guo
authors.initials: RG
authors.lastname: Fu
mesh_terms: D000818:Animals; D001552:Benzazepines / Q000494:pharmacology; D049109:Cell Proliferation; D017630:Connexins / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D050527:Mesangial Cells / Q000187:drug effects* / Q000378:metabolism; D012333:RNA, Messenger; D051381:Rats; D000097004:Gap Junction delta-2 Protein
chemical_list: D001552:Benzazepines; D017630:Connexins; D004365:Drugs, Chinese Herbal; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D000097004:Gap Junction delta-2 Protein; C044946:benazepril