title: Tobacco dust induced genotoxicity as an occupational hazard in workers of bidi making cottage industry of central India.
abstract: BACKGROUND
  To explore genotoxicity in bidi rollers occupationally exposed to bidi tobacco dust.
  
  
  OBJECTIVE
  To assess the extent of genotoxicity of tobacco dust to bidi rollers of Jabalpur, Madhya Pradesh, India and cytotoxicity of bidi tobacco extract.
  
  
  METHODS
  Blood samples from 31 bidi rollers and 30 controls taken after written informed consent were analyzed for chromosome aberrations (CA) and comet assay.
  
  
  METHODS
  Genotoxicity was studied by CA in cultured peripheral blood lymphocytes of bidi rollers and the deoxyribonucleic acid (DNA) damage studies were done by comet assay of their blood. The toxicity of bidi tobacco extract to normal human lymphocytes was studied by MMT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay as drop in viability.
  
  
  METHODS
  Student's t-test and DMRT.
  
  
  RESULTS
  There is a general trend of increase in CA% of both in exposed and control groups with age, but in every group the bidi rollers have a significantly higher CA% than the controls. The CA % is also directly related to exposure. The comet assay findings reveal that the mean comet length and tail length increases with exposure time. The toxicity of bidi tobacco extract (TE) to normal human lymphocytes was tested in vitro by 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay at 2 h of incubation. The trend of drop in viability with increasing concentrations of TE was clearly evident from the data from four donors in spite of their individual differences in viability.
  
  
  CONCLUSIONS
  The results obtained in this investigation indicate that bidi rollers seem to be facing the occupational hazard of genotoxicity due to handling bidi tobacco and inhalation of tobacco dust. They should be advised to work under well-ventilated conditions.
authors.affiliation: Department of Zoology and Biotechnology, St. Aloysius College (Autonomous), Jabalpur, Madhya Pradesh, India.
authors.forename: Asha
authors.initials: A
authors.lastname: Khanna
authors.affiliation: Department of Zoology and Biotechnology, St. Aloysius College (Autonomous), Jabalpur, Madhya Pradesh, India.
authors.forename: Daya Shankar
authors.initials: DS
authors.lastname: Gautam
authors.affiliation: Department of Botany and Microbiology, St. Aloysius College (Autonomous), Jabalpur, Madhya Pradesh, India.
authors.forename: Mamta
authors.initials: M
authors.lastname: Gokhale
authors.affiliation: Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science and Animal Husbandry, Nanaji Deshmukh Veterinary Science University, Jabalpur, Madhya Pradesh, India.
authors.forename: Salil Kumar
authors.initials: SK
authors.lastname: Jain
keywords: Chromosome aberration; MTT assay; comet assay; genotoxicity in bidi rollers; tobacco dust
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