title: A novel hydrogen peroxide biosensor based on hemoglobin-collagen-CNTs composite nanofibers.
abstract: In this paper, carbon nanotubes (CNTs) were successfully incorporated in the composite composed of hemoglobin (Hb) and collagen using co-electrospinning technology. The formed Hb-collagen-CNTs composite nanofibers possessed distinct advantage of three-dimensional porous structure, biocompatibility and excellent stability. The Hb immobilized in the electrospun nanofibers retained its natural structure and the heterogeneous electron transfer rate constant (ks) of the direct electron transfer between Hb and electrodes was 5.3s(-1). In addition, the electrospun Hb-collagen-CNTs nanofibers modified electrodes showed good electrocatalytic properties toward H2O2 with a detection limit of 0.91μM (signal-to-noise ratio of 3) and the apparent Michaelis-Menten constant (Km(app)) of 32.6μM.
authors.affiliation: Key Laboratory of Green Chemical Engineering and Technology of College of Heilongjiang Province, College of Chemical and Environmental Engineering, Harbin University of Science and Technology, Harbin, 150080, China. Electronic address: lijia@hrbust.edu.cn.
authors.forename: J
authors.initials: J
authors.lastname: Li
authors.affiliation: Key Laboratory of Green Chemical Engineering and Technology of College of Heilongjiang Province, College of Chemical and Environmental Engineering, Harbin University of Science and Technology, Harbin, 150080, China.
authors.forename: H
authors.initials: H
authors.lastname: Mei
authors.affiliation: Center for Biomedical Materials and Engineering, Harbin Engineering University, Harbin 150001, China. Electronic address: zhengwei75@126.com.
authors.forename: W
authors.initials: W
authors.lastname: Zheng
authors.affiliation: Key Laboratory of Green Chemical Engineering and Technology of College of Heilongjiang Province, College of Chemical and Environmental Engineering, Harbin University of Science and Technology, Harbin, 150080, China.
authors.forename: P
authors.initials: P
authors.lastname: Pan
authors.affiliation: Key Laboratory of Green Chemical Engineering and Technology of College of Heilongjiang Province, College of Chemical and Environmental Engineering, Harbin University of Science and Technology, Harbin, 150080, China.
authors.forename: X J
authors.initials: XJ
authors.lastname: Sun
authors.affiliation: Key Laboratory of Green Chemical Engineering and Technology of College of Heilongjiang Province, College of Chemical and Environmental Engineering, Harbin University of Science and Technology, Harbin, 150080, China.
authors.forename: F
authors.initials: F
authors.lastname: Li
authors.affiliation: Center for Biomedical Materials and Engineering, Harbin Engineering University, Harbin 150001, China.
authors.forename: F
authors.initials: F
authors.lastname: Guo
authors.affiliation: Center for Biomedical Materials and Engineering, Harbin Engineering University, Harbin 150001, China.
authors.forename: H M
authors.initials: HM
authors.lastname: Zhou
authors.affiliation: Center for Biomedical Materials and Engineering, Harbin Engineering University, Harbin 150001, China.
authors.forename: J Y
authors.initials: JY
authors.lastname: Ma
authors.affiliation: Center for Biomedical Materials and Engineering, Harbin Engineering University, Harbin 150001, China.
authors.forename: X X
authors.initials: XX
authors.lastname: Xu
authors.affiliation: Center for Biomedical Materials and Engineering, Harbin Engineering University, Harbin 150001, China; Department of Materials Science and Engineering, College of Engineering, Peking University, Beijing 100871, China.
authors.forename: Y F
authors.initials: YF
authors.lastname: Zheng
mesh_terms: D000818:Animals; D015374:Biosensing Techniques; D002417:Cattle; D003094:Collagen / Q000737:chemistry* / Q000648:ultrastructure; D055664:Electrochemical Techniques; D004566:Electrodes; D004583:Electrons; D006454:Hemoglobins / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000032:analysis*; D055767:Immobilized Proteins / Q000737:chemistry; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure
chemical_list: D006454:Hemoglobins; D055767:Immobilized Proteins; D037742:Nanotubes, Carbon; D003094:Collagen; D006861:Hydrogen Peroxide
keywords: Biosensor; Electrochemistry; Electrospinning; Hemoglobin-collagen-CNTs nanofibers