title: Quantitative proteomics analysis of altered protein expression in the placental villous tissue of early pregnancy loss using isobaric tandem mass tags.
abstract: Many pregnant women suffer miscarriages during early gestation, but the description of these early pregnancy losses (EPL) can be somewhat confusing because of the complexities of early development. Thus, the identification of proteins with different expression profiles related to early pregnancy loss is essential for understanding the comprehensive pathophysiological mechanism. In this study, we report a gel-free tandem mass tags- (TMT-) labeling based proteomic analysis of five placental villous tissues from patients with early pregnancy loss and five from normal pregnant women. The application of this method resulted in the identification of 3423 proteins and 19647 peptides among the patient group and the matched normal control group. Qualitative and quantitative proteomic analysis revealed 51 proteins to be differentially abundant between the two groups (≥ 1.2-fold, Student's t-test, P < 0.05). To obtain an overview of the biological functions of the proteins whose expression levels altered significantly in EPL group, gene ontology analysis was performed. We also investigated the twelve proteins with a difference over 1.5-fold using pathways analysis. Our results demonstrate that the gel-free TMT-based proteomic approach allows the quantification of differences in protein expression levels, which is useful for obtaining molecular insights into early pregnancy loss.
authors.affiliation: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing 210029, China ; Department of Histology and Embryology, Nanjing Medical University, Nanjing 210029, China.
authors.forename: Xiaobei
authors.initials: X
authors.lastname: Ni
authors.affiliation: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing 210029, China ; Nanjing Maternity and Child Health Hospital, Nanjing Medical University, Nanjing 210029, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.affiliation: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing 210029, China.
authors.forename: Yueshuai
authors.initials: Y
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing 210029, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing 210029, China ; Department of Histology and Embryology, Nanjing Medical University, Nanjing 210029, China.
authors.forename: Xuejiang
authors.initials: X
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing 210029, China ; Nanjing Maternity and Child Health Hospital, Nanjing Medical University, Nanjing 210029, China.
authors.forename: Chun
authors.initials: C
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing 210029, China.
authors.forename: Min
authors.initials: M
authors.lastname: Lin
authors.affiliation: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing 210029, China ; Department of Histology and Embryology, Nanjing Medical University, Nanjing 210029, China.
authors.forename: Zuomin
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: Nanjing Maternity and Child Health Hospital, Nanjing Medical University, Nanjing 210029, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Shen
authors.affiliation: Nanjing Maternity and Child Health Hospital, Nanjing Medical University, Nanjing 210029, China.
authors.forename: Xirong
authors.initials: X
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing 210029, China ; Nanjing Maternity and Child Health Hospital, Nanjing Medical University, Nanjing 210029, China.
authors.forename: Xiufeng
authors.initials: X
authors.lastname: Ling
authors.affiliation: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing 210029, China ; Department of Histology and Embryology, Nanjing Medical University, Nanjing 210029, China.
authors.forename: Ran
authors.identifier: 0000-0001-5166-127X
authors.initials: R
authors.lastname: Huo
mesh_terms: D000022:Abortion, Spontaneous / Q000378:metabolism / Q000473:pathology; D002824:Chorionic Villi / Q000378:metabolism*; D005260:Female; D006801:Humans; D011247:Pregnancy; D014176:Protein Biosynthesis; D040901:Proteomics; D053719:Tandem Mass Spectrometry
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