title: The effect of polyamines on the binding of anti-DNA antibodies from patients with SLE and normal human subjects.
abstract: Antibodies to DNA (anti-DNA) are the serological hallmark of systemic lupus erythematosus (SLE). To elucidate specificity further, the effect of polyamines on the binding of anti-DNA antibodies from patients with lupus was tested by ELISA to calf thymus (CT) DNA; we also assessed the binding of plasmas of patients and normal human subjects (NHS) to Micrococcus luteus (MC) DNA. As these studies showed, spermine can dose-dependently inhibit SLE anti-DNA binding to CT DNA and can promote dissociation of preformed immune complexes. With MC DNA as antigen, spermine failed to inhibit the NHS anti-DNA binding. Studies using plasmas adsorbed to a CT DNA cellulose affinity indicated that SLE plasmas are mixtures of anti-DNA that differ in inhibition by spermine and binding to conserved and non-conserved determinants. Together, these studies demonstrate that spermine can influence the binding of anti-DNA autoantibodies and may contribute to the antigenicity of DNA.
authors.affiliation: Department of Rheumatology, Qilu Hospital, Shandong University, Jinan, China; Medical Research Service, Durham Veterans Administration Medical Center, Durham, NC, USA.
authors.forename: Xiao
authors.initials: X
authors.lastname: Wang
authors.affiliation: Medical Research Service, Durham Veterans Administration Medical Center, Durham, NC, USA; Duke University Medical Center, Durham, NC, USA.
authors.forename: Nancy A
authors.initials: NA
authors.lastname: Stearns
authors.affiliation: Department of Rheumatology, Qilu Hospital, Shandong University, Jinan, China.
authors.forename: Xingfu
authors.initials: X
authors.lastname: Li
authors.affiliation: Medical Research Service, Durham Veterans Administration Medical Center, Durham, NC, USA; Duke University Medical Center, Durham, NC, USA. Electronic address: dpiset@acpub.duke.edu.
authors.forename: David S
authors.initials: DS
authors.lastname: Pisetsky
mesh_terms: D000974:Antibodies, Antinuclear / Q000276:immunology* / Q000378:metabolism; D000918:Antibody Specificity / Q000276:immunology; D000936:Antigen-Antibody Complex / Q000276:immunology; D016022:Case-Control Studies; D004247:DNA / Q000276:immunology / Q000378:metabolism; D004269:DNA, Bacterial / Q000276:immunology / Q000378:metabolism; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000276:immunology* / Q000378:metabolism; D011073:Polyamines / Q000494:pharmacology*; D011485:Protein Binding / Q000187:drug effects; D013095:Spermidine / Q000494:pharmacology
chemical_list: D000974:Antibodies, Antinuclear; D000936:Antigen-Antibody Complex; D004269:DNA, Bacterial; D011073:Polyamines; D004247:DNA; C427203:calf thymus DNA; D013095:Spermidine
keywords: Anti-DNA antibody;; Antigenicity;; Polyamines; SLE;; Spermine;
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