title: Protective effects of kaempferol on lipopolysaccharide-induced mastitis in mice.
abstract: Kaempferol isolated from the root of Zingiberaceae plants galangal and other Chinese herbal medicines have been reported to have anti-inflammatory properties. However, the anti-inflammatory effects of kaempferol on lipopolysaccharide (LPS)-induced mastitis are unknown and their underlying molecular mechanisms remain to be explored. The aim of this study was to evaluate the effects of kaempferol on LPS-induced mouse mastitis. The mouse model of mastitis was induced by injection of LPS through the duct of mammary gland. Kaempferol was injected 1 h before and 12 h after induction of LPS intraperitoneally. The present results showed that kaempferol markedly reduced infiltration of neutrophilic granulocyte, activation of myeloperoxidase (MPO), expression of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β) in a dose-dependent manner, which were increased in LPS-induced mouse mastitis. Furthermore, kaempferol suppressed the phosphorylation of nuclear factor-κB (NF-κB) p65 subunit and the degradation of its inhibitor IκBα. All results suggest that anti-inflammatory effects of kaempferol against the LPS-induced mastitis possibly through inhibition of the NF-κB signaling pathway. Kaempferol may be a potential therapeutic agent for mastitis.
authors.affiliation: Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, Jilin Province, 130062, People's Republic of China.
authors.forename: Rongfeng
authors.initials: R
authors.lastname: Cao
authors.forename: Kaiqiang
authors.initials: K
authors.lastname: Fu
authors.forename: Xiaopei
authors.initials: X
authors.lastname: Lv
authors.forename: Weishi
authors.initials: W
authors.lastname: Li
authors.forename: Naisheng
authors.initials: N
authors.lastname: Zhang
mesh_terms: D000818:Animals; D005260:Female; D018836:Inflammation Mediators / Q000037:antagonists & inhibitors / Q000378:metabolism*; D044949:Kaempferols / Q000494:pharmacology / Q000627:therapeutic use*; D008070:Lipopolysaccharides / Q000633:toxicity*; D008297:Male; D008413:Mastitis / Q000139:chemically induced / Q000378:metabolism* / Q000517:prevention & control*; D051379:Mice; D008807:Mice, Inbred BALB C; D011897:Random Allocation
chemical_list: D018836:Inflammation Mediators; D044949:Kaempferols; D008070:Lipopolysaccharides; C006552:kaempferol
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