title: Improved probiotic viability in stress environments with post-culture of alginate-chitosan microencapsulated low density cells.
abstract: In this study, probiotics (Saccharomyces cerevisiae Y235) were entrapped in alginate-chitosan microcapsules by emulsification/internal gelation technique. Two different encapsulation patterns were established as directly entrapped high density cells (dEHDC) and entrapped low density cells with culture (ELDCwc). The performance of microencapsulated cells, with free cells (FC) as control, was investigated against sequential stress environments of freeze-drying, storage, and simulated gastrointestinal fluids. After being freeze-dried without cryoprotectant, the survival rate of ELDCwc (14.33%) was significantly higher than 10.00% of dEHDC, and 0.05% of FC. The lower temperature (-20°C) and ELDCwc pattern were beneficial for keeping viable cells at 7.00 logCFU g(-1) after 6 months. Furthermore, the ELDCwc microcapsule maintained viable cells of 6.29 logCFU g(-1) after incubation in SGF and SIF. These studies demonstrated that the pattern of entrapped low density cells with culture was an effective and superior technique of resisting harmful stress environments.
authors.affiliation: Laboratory of Biomedical Materials Engineering, Dalian Institute of Chemical Physics(DICP), Chinese Academy of Sciences(CAS), Dalian 116023, PR China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Huiyi
authors.initials: H
authors.lastname: Song
authors.affiliation: Laboratory of Biomedical Materials Engineering, Dalian Institute of Chemical Physics(DICP), Chinese Academy of Sciences(CAS), Dalian 116023, PR China. Electronic address: yuwt@dicp.ac.cn.
authors.forename: Weiting
authors.initials: W
authors.lastname: Yu
authors.affiliation: College of Environment and Chemical Engineering, Dalian University, Dalian Economic Technological Development Zone, Dalian 116622, PR China.
authors.forename: Xiudong
authors.initials: X
authors.lastname: Liu
authors.affiliation: Laboratory of Biomedical Materials Engineering, Dalian Institute of Chemical Physics(DICP), Chinese Academy of Sciences(CAS), Dalian 116023, PR China. Electronic address: maxj@dicp.ac.cn.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Ma
mesh_terms: D000464:Alginates / Q000737:chemistry*; D002214:Capsules / Q000737:chemistry*; D048271:Chitosan / Q000737:chemistry*; D004339:Drug Compounding; D004355:Drug Stability; D005612:Freeze Drying; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D019936:Probiotics; D012441:Saccharomyces cerevisiae / Q000502:physiology; D013696:Temperature
chemical_list: D000464:Alginates; D002214:Capsules; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D048271:Chitosan
keywords: Microencapsulated cell culture; Probiotic viability; Resistance to stress environments