title: The sense is in the fingertips: The distal end controls filopodial mechanics and dynamics in response to external stimuli.
abstract: Small hair-like cell protrusions, called filopodia, often establish adhesive contacts with the cellular surroundings with a subsequent build up of retraction force. This process seems to be important for cell migration, embryonic development, wound healing, and pathogenic infection pathways. We have shown that filopodial tips are able to sense adhesive contact and, as a consequence, locally reduce actin polymerization speed. This induces filopodial retraction via forces generated by the cell membrane tension and by the filopodial actin shaft that is constantly pulled rearwards via the retrograde flow of actin at the base. The tip is also the weakest point of actin-based force transduction. Forces higher than 15 pN can disconnect the actin shaft from the membrane, which increases actin polymerization at the tip. Together, this points toward the tip as a mechano-chemical sensing and steering unit for filopodia, and it calls for a better understanding of the molecular mechanisms involved.
authors.affiliation: Institut Curie; Centre de Recherche; Paris, France ; CNRS; UMR 168; Paris, France ; Université Pierre et Marie Curie; Paris, France ; CelTisPhyBioLabex and Paris Sciences et Lettres; Paris, France.
authors.forename: Thomas
authors.initials: T
authors.lastname: Bornschlögl
authors.affiliation: Institut Curie; Centre de Recherche; Paris, France ; CNRS; UMR 168; Paris, France ; Université Pierre et Marie Curie; Paris, France ; CelTisPhyBioLabex and Paris Sciences et Lettres; Paris, France.
authors.forename: Patricia
authors.initials: P
authors.lastname: Bassereau
keywords: cytoskeleton; filopodia; lamellipodia; membrane tension; pulling force; retrograde flow
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