title: Arabidopsis HRE1α, a splicing variant of AtERF73/HRE1, functions as a nuclear transcription activator in hypoxia response and root development.
abstract: HRE1α shows transcriptional activation activity in its C-terminal region via GCC box but not DRE/CRT and plays an important role in root development via root meristem cell division regulation. AtERF73/HRE1 protein, a member of the Arabidopsis AP2/ERF family, contains a conserved AP2/ERF DNA-binding domain. Here, we studied the molecular function of HRE1α, a splicing variant of AtERF73/HRE1, as well as its role in root development. HRE1α-overexpressing transgenic plants (OXs) showed tolerance to submergence. HRE1α showed transcriptional activation activity via GCC box but not DRE/CRT. The 121-211 aa region of HRE1α was responsible for the transcriptional activation activity, and the region was conserved among homologs of other species but was not found in other Arabidopsis proteins. HRE1α OXs showed increased primary root length due to elevated root cell division. Our results suggest that HRE1α functions as a transcription activator in the nucleus, and plays an important role in root development through regulation of root meristem cell division.
authors.affiliation: Department of Molecular Biology, Pusan National University, Busan, 609-735, Korea.
authors.forename: Hye-Yeon
authors.initials: HY
authors.lastname: Seok
authors.forename: Vaishali N
authors.initials: VN
authors.lastname: Tarte
authors.forename: Sun-Young
authors.initials: SY
authors.lastname: Lee
authors.forename: Hee-Yeon
authors.initials: HY
authors.lastname: Park
authors.forename: Yong-Hwan
authors.initials: YH
authors.lastname: Moon
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D002455:Cell Division; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D018519:Meristem / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010100:Oxygen / Q000378:metabolism*; D010802:Phylogeny; D018517:Plant Roots / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D030821:Plants, Genetically Modified; D020033:Protein Isoforms; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013312:Stress, Physiological; D015534:Trans-Activators / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D015533:Transcriptional Activation
chemical_list: D029681:Arabidopsis Proteins; C000596383:HRE1 protein, Arabidopsis; D020033:Protein Isoforms; D015534:Trans-Activators; D014157:Transcription Factors; D010100:Oxygen
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