title: Capillary dilation and rarefaction are correlated with intracapillary inflammation in antibody-mediated rejection.
abstract: Antibody-mediated rejection (ABMR) remains one of the major causes of graft loss after renal transplantation. It is dominated by endothelial damage in microcirculation. Clarifying the mechanism of microcirculating damage is obviously a key step to understand the pathogenesis of ABMR. Here we characterized capillary variation in ABMR and its possible mechanisms. Compared with T cell-mediated rejection and stable grafts, there was a significant dilation and rarefaction in peritubular capillaries (PTCs) of the ABMR group; Image-Pro Plus revealed a significantly larger intra-PTC area. Interestingly, the dilation of PTCs was strongly correlated with the intra-PTC cell counting. Moreover, peritubular capillary inflammation is correlated with in situ T-bet expression, and there was a good correlation between the intra-PTC expression of T-bet and the PTC diameter. HIF-1α up-regulation could be observed in ABMR but it was not necessary for capillary dilation. In general, ABMR is characterized with early capillary dilation and rarefaction; our data confirmed that the dilation is strongly correlated with intracapillary inflammation, which in turn is correlated with in situ T-bet expression. T-bet plays an important role in the development of microcirculating injury, and thus it is a potential target for the treatment of ABMR.
authors.affiliation: National Clinical Research Center of Kidney Diseases, Jinling Hospital, Nanjing University Clinical School of Medicine, 305 East Zhong Shan Road, Nanjing 210002, China.
authors.forename: Xue
authors.identifier: 0000-0001-7073-7532
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Renal Transplantation, The Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510760, China.
authors.forename: Qiquan
authors.initials: Q
authors.lastname: Sun
authors.affiliation: National Clinical Research Center of Kidney Diseases, Jinling Hospital, Nanjing University Clinical School of Medicine, 305 East Zhong Shan Road, Nanjing 210002, China.
authors.forename: Mingchao
authors.initials: M
authors.lastname: Zhang
authors.affiliation: National Clinical Research Center of Kidney Diseases, Jinling Hospital, Nanjing University Clinical School of Medicine, 305 East Zhong Shan Road, Nanjing 210002, China.
authors.forename: Kenan
authors.initials: K
authors.lastname: Xie
authors.affiliation: National Clinical Research Center of Kidney Diseases, Jinling Hospital, Nanjing University Clinical School of Medicine, 305 East Zhong Shan Road, Nanjing 210002, China.
authors.forename: Jinsong
authors.initials: J
authors.lastname: Chen
authors.affiliation: National Clinical Research Center of Kidney Diseases, Jinling Hospital, Nanjing University Clinical School of Medicine, 305 East Zhong Shan Road, Nanjing 210002, China.
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Liu
mesh_terms: D000328:Adult; D000920:Antibody-Dependent Cell Cytotoxicity / Q000276:immunology*; D002196:Capillaries / Q000276:immunology* / Q000378:metabolism / Q000473:pathology*; D005260:Female; D006084:Graft Rejection / Q000209:etiology* / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism; D007150:Immunohistochemistry; D007249:Inflammation / Q000276:immunology* / Q000378:metabolism / Q000473:pathology*; D016030:Kidney Transplantation; D008297:Male; D008833:Microcirculation / Q000276:immunology; D008875:Middle Aged; D020825:T-Box Domain Proteins / Q000235:genetics / Q000378:metabolism; D014184:Transplantation, Homologous; D000098815:T-bet Transcription Factor
chemical_list: C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D020825:T-Box Domain Proteins; D000098815:T-bet Transcription Factor
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