title: Determination of bevantolol in human plasma using liquid chromatography-electrospray ionization tandem mass spectrometry and its application to a bioequivalence study.
abstract: A liquid chromatography-electrospray ionization tandem mass spectrometry method was established and validated for the determination of bevantolol in human plasma using propranolol as the internal standard. The optimal chromatographic behavior of bevantolol and propranolol was achieved on a Welch Ultimate XB-C18 column (5 μm, 150 mm × 2.1mm, Maryland, USA) with a mobile phase of acetonitrile-water (40:60, v/v) containing 10mM ammonium acetate and 0.1% formic acid. The mass spectrometer was operated in selected reaction monitoring mode using the transition m/z 346.1>165.1 for bevantolol and m/z 260.3>116.1 for propranolol. Sample preparation was carried out through protein precipitation with acetonitrile. The calibration curves were linear over the range of 5.00-1,000 ng/ml. The intra- and inter-day precisions were less than 6.7% and 6.6%, respectively. This method was successfully applied to the bioequivalence study of two kinds of bevantolol hydrochloride tablets in 24 Chinese male volunteers in fasting and postprandial experiment.
authors.affiliation: College of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Li
authors.initials: L
authors.lastname: Ren
authors.affiliation: School of Chemistry and Engineering, Wuhan University of Technology, Wuhan 430070, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Wang
authors.affiliation: School of Chemistry and Engineering, Wuhan University of Technology, Wuhan 430070, China.
authors.forename: Yiceng
authors.initials: Y
authors.lastname: Lou
authors.affiliation: Shanghai Haini Pharmaceutical Co., Ltd, Yangtze River Pharmaceutical Group, 3999 Hunan Road, Pudong, Shanghai 201318, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Zheng
authors.affiliation: Department of Pharmacy, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Avenue, Wuhan 430030, China. Electronic address: zhenghengh@yahoo.com.
authors.forename: Heng
authors.initials: H
authors.lastname: Zheng
authors.affiliation: College of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. Electronic address: cpyin888@163.com.
authors.forename: Chunping
authors.initials: C
authors.lastname: Yin
mesh_terms: D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D006801:Humans; D008297:Male; D011412:Propanolamines / Q000097:blood / Q000493:pharmacokinetics*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013810:Therapeutic Equivalency
chemical_list: D011412:Propanolamines; C021148:bevantolol
keywords: Bevantolol; Bioequivalence; LC–MS/MS