title: RNAi targeting GPR4 influences HMEC-1 gene expression by microarray analysis.
abstract: G-protein coupled receptor 4 (GPR4) belongs to a protein family comprised of 3 closely related G protein-coupled receptors. Recent studies have shown that GPR4 plays important roles in angiogenesis, proton sensing, and regulating tumor cells as an oncogenic gene. How GPR4 conducts its functions? Rare has been known. In order to detect the genes related to GPR4, microarray technology was employed. GPR4 is highly expressed in human vascular endothelial cell HMEC-1. Small interfering RNA against GPR4 was used to knockdown GPR4 expression in HMEC-1. Then RNA from the GPR4 knockdown cells and control cells were analyzed through genome microarray. Microarray results shown that among the whole genes and expressed sequence tags, 447 differentially expressed genes were identified, containing 318 up-regulated genes and 129 down-regulated genes. These genes whose expression dramatically changed may be involved in the GPR4 functions. These genes were related to cell apoptosis, cytoskeleton and signal transduction, cell proliferation, differentiation and cell-cycle regulation, gene transcription and translation and cell material and energy metabolism.
authors.affiliation: Cancer Center Xi'an, Shaanxi Province, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Ren
authors.affiliation: Department of Imaging, First Affiliated Hospital of Xi'an Jiaotong University Xi'an, Shaanxi Province, China.
authors.forename: Yuelang
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Medical School, Xi'an Jiaotong University Xi'an, Shaanxi Province, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Cai
authors.affiliation: Second Affiliated Hospital of Xi'an Jiaotong University Xi'an, Shaanxi Province, China.
authors.forename: Hongbing
authors.initials: H
authors.lastname: Ma
authors.affiliation: Cancer Center Xi'an, Shaanxi Province, China.
authors.forename: Dongli
authors.initials: D
authors.lastname: Zhao
authors.affiliation: Cancer Center Xi'an, Shaanxi Province, China.
authors.forename: Xiaozhi
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Second Affiliated Hospital of Xi'an Jiaotong University Xi'an, Shaanxi Province, China.
authors.forename: Zongfang
authors.initials: Z
authors.lastname: Li
authors.affiliation: Cancer Center Xi'an, Shaanxi Province, China.
authors.forename: Shufeng
authors.initials: S
authors.lastname: Wang
authors.affiliation: Cancer Center Xi'an, Shaanxi Province, China.
authors.forename: Jiangsheng
authors.initials: J
authors.lastname: Wang
authors.affiliation: Cancer Center Xi'an, Shaanxi Province, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Liu
authors.affiliation: Cancer Center Xi'an, Shaanxi Province, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Li
authors.affiliation: Cancer Center Xi'an, Shaanxi Province, China.
authors.forename: Jiansheng
authors.initials: J
authors.lastname: Qian
authors.affiliation: Cancer Center Xi'an, Shaanxi Province, China.
authors.forename: Hongxia
authors.initials: H
authors.lastname: Wei
authors.affiliation: Cancer Center Xi'an, Shaanxi Province, China.
authors.forename: Liying
authors.initials: L
authors.lastname: Niu
authors.affiliation: Cancer Center Xi'an, Shaanxi Province, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Medical School, Xi'an Jiaotong University Xi'an, Shaanxi Province, China.
authors.forename: Lisha
authors.initials: L
authors.lastname: Xiao
authors.affiliation: Medical School, Xi'an Jiaotong University Xi'an, Shaanxi Province, China.
authors.forename: Muyang
authors.initials: M
authors.lastname: Ding
authors.affiliation: Mercer University School of Medicine USA.
authors.forename: Shiwen
authors.initials: S
authors.lastname: Jiang
keywords: GPR4; RNAi; microarray
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