title: Functional identification of Proteus mirabilis eptC gene encoding a core lipopolysaccharide phosphoethanolamine transferase.
abstract: By comparison of the Proteus mirabilis HI4320 genome with known lipopolysaccharide (LPS) phosphoethanolamine transferases, three putative candidates (PMI3040, PMI3576, and PMI3104) were identified. One of them, eptC (PMI3104) was able to modify the LPS of two defined non-polar core LPS mutants of Klebsiella pneumoniae that we use as surrogate substrates. Mass spectrometry and nuclear magnetic resonance showed that eptC directs the incorporation of phosphoethanolamine to the O-6 of L-glycero-D-mano-heptose II. The eptC gene is found in all the P. mirabilis strains analyzed in this study. Putative eptC homologues were found for only two additional genera of the Enterobacteriaceae family, Photobacterium and Providencia. The data obtained in this work supports the role of the eptC (PMI3104) product in the transfer of PEtN to the O-6 of L,D-HepII in P. mirabilis strains.
authors.affiliation: Department Microbiology and Parasitology, Faculty Pharmacy, University of Barcelona, Av. Joan XXIII s/n, 08028 Barcelona, Spain. eaquilini@ub.edu.
authors.forename: Eleonora
authors.initials: E
authors.lastname: Aquilini
authors.affiliation: Department Microbiology, Faculty Biology, University Barcelona, Diagonal 643, 08071 Barcelona, Spain. smerino@ub.edu.
authors.forename: Susana
authors.initials: S
authors.lastname: Merino
authors.affiliation: Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow 119991, Russia. yknirel@gmail.com.
authors.forename: Yuriy A
authors.initials: YA
authors.lastname: Knirel
authors.affiliation: Department Microbiology and Parasitology, Faculty Pharmacy, University of Barcelona, Av. Joan XXIII s/n, 08028 Barcelona, Spain.
authors.forename: Miguel
authors.initials: M
authors.lastname: Regué
authors.affiliation: Department Microbiology, Faculty Biology, University Barcelona, Diagonal 643, 08071 Barcelona, Spain. jtomas@ub.edu.
authors.forename: Juan M
authors.initials: JM
authors.lastname: Tomás
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000145:classification / Q000378:metabolism*; D002240:Carbohydrate Sequence; D004982:Ethanolaminephosphotransferase / Q000737:chemistry / Q000145:classification / Q000378:metabolism*; D016680:Genome, Bacterial; D007711:Klebsiella pneumoniae / Q000378:metabolism; D008070:Lipopolysaccharides / Q000737:chemistry / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D010802:Phylogeny; D011513:Proteus mirabilis / Q000201:enzymology / Q000235:genetics; D016415:Sequence Alignment; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D001426:Bacterial Proteins; D008070:Lipopolysaccharides; D004982:Ethanolaminephosphotransferase
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