title: Anti-inflammatory triterpenoids from the stems of Microtropis fokienensis.
abstract: Three new ursane- and four new oleanane- type triterpenoids 1-7 were isolated, along with six known compounds 8-13, from the methanolic extract of Microtropis fokienensis. All structures were elucidated by mass and NMR spectroscopic methods. The isolates 4-10 and known compounds 14-17 that were previously isolated from this material were evaluated for anti-inflammatory activity based on effects against superoxide anion generation and elastase release by neutrophils in response to fMLP/CB. 11α,30-Dihydroxy-2,3-seco-olean-12-en-2,3-dioic anhydride (7) was the first triterpene anhydride from the genus of Microtropis to have the ring A expanded to a seven-membered ring; it showed significant anti-inflammatory activity against superoxide anion generation and elastase release. Unexpectedly, 30-hydroxy-2,3-seco-lup-20(29)-ene-2,3-dioic acid (17) showed the best effect against superoxide anion generation and elastase release with IC50 values of 0.06±0.01 and 1.03±0.35 µg/mL, respectively. Compound 17 had a dioic acid function, and compound 7 had an anhydride function modification in ring A; both showed promising activity in the target assays.
authors.affiliation: School of Chinese Medicine for Post Baccalaureate, College of Medicine, I Shou University, Kaohsiung 82445, Taiwan. fantasysp@isu.edu.tw.
authors.forename: I-Hsiao
authors.initials: IH
authors.lastname: Chen
authors.affiliation: Department of Botanicals, Medical and Pharmaceutical Industry Technology and Development Center, New Taipei City 248, Taiwan. ycdu0626@gmail.com.
authors.forename: Ying-Chi
authors.initials: YC
authors.lastname: Du
authors.affiliation: Graduate Institute of Natural Products, College of Medicine, Chang Gung University, Taoyuan 333, Taiwan. htl@mail.cgu.edu.tw.
authors.forename: Tsong-Long
authors.initials: TL
authors.lastname: Hwang
authors.affiliation: Department of Biomedical Engineering, College of Medicine, I Shou University, Kaohsiung 82445, Taiwan. ifen@isu.edu.tw.
authors.forename: I-Fen
authors.initials: IF
authors.lastname: Chen
authors.affiliation: School of Pharmacy, College of Pharmacy, China Medical University, Taichung 404, Taiwan. lanyh@mail.cmu.edu.tw.
authors.forename: Yu-Hsuan
authors.initials: YH
authors.lastname: Lan
authors.affiliation: National Museum of Natural Science, Taichung 404, Taiwan. hfyen@mail.nmns.edu.tw.
authors.forename: Hsin-Fu
authors.initials: HF
authors.lastname: Yen
authors.affiliation: Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan. aaronfrc@kmu.edu.tw.
authors.forename: Fang-Rong
authors.initials: FR
authors.lastname: Chang
authors.affiliation: School of Pharmacy, College of Pharmacy, China Medical University, Taichung 404, Taiwan. yachwu@mail.cmu.edu.tw.
authors.forename: Yang-Chang
authors.initials: YC
authors.lastname: Wu
mesh_terms: D000328:Adult; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D029753:Celastraceae / Q000737:chemistry*; D006801:Humans; D000432:Methanol; D015394:Molecular Structure; D009504:Neutrophils / Q000187:drug effects / Q000201:enzymology; D009828:Oleanolic Acid / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry*; D012997:Solvents; D013329:Structure-Activity Relationship; D013481:Superoxides / Q000037:antagonists & inhibitors; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D053978:Pentacyclic Triterpenes
chemical_list: D000893:Anti-Inflammatory Agents; D000432:Methanol; D009828:Oleanolic Acid; D010196:Pancreatic Elastase; D010936:Plant Extracts; D012997:Solvents; D013481:Superoxides; D014315:Triterpenes; C413246:oleanane; C000606873:ursane; D053978:Pentacyclic Triterpenes
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