title: Growth of osteosarcoma cells in a three-dimensional bone-like matrix alters their susceptibility to adeno-associated virus.
abstract: Osteosarcoma cells U2OS are partially susceptible to adeno-associated virus (AAV)-2 infection, allowing efficient synthesis of Rep proteins and, in a low percentage of cells, capsid production. It is not clear if this partial susceptibility to infection is due to the bone-cell-like nature of these cells or is a result of their transformed properties. Here, we grew osteosarcoma cells in a biomimetic three-dimensional bone-like matrix composed of calcium phosphate and chitosan, and tested whether this would increase or reduce their permissiveness to virus. The osteosarcoma cells grew in the matrix and began to express the alkaline phosphatase bone cell differentiation marker. This was accompanied by a block to their infection by AAV, as indicated by Rep and capsid production. Infection of cells growing in three-dimensional tissue-like matrices could be, in a wider context, a practical way to mimic in vivo conditions.
authors.affiliation: Swiss Federal Institute of Technology Lausanne (EPFL), Faculty of Life Sciences, EPFL SV-DEC PH-SV, SV 3516 - Station 19, 1015 Lausanne, Switzerland.
authors.forename: Sangeeta
authors.initials: S
authors.lastname: Dey
authors.affiliation: Swiss Federal Institute of Technology Lausanne (EPFL), Faculty of Life Sciences, EPFL SV-DEC PH-SV, SV 3516 - Station 19, 1015 Lausanne, Switzerland.
authors.forename: Leila
authors.initials: L
authors.lastname: Laredj
authors.affiliation: Swiss Federal Institute of Technology Lausanne (EPFL), Faculty of Life Sciences, EPFL SV-DEC PH-SV, SV 3516 - Station 19, 1015 Lausanne, Switzerland.
authors.forename: Katarina
authors.initials: K
authors.lastname: Damjanovic
authors.affiliation: Swiss Federal Institute of Technology Lausanne (EPFL), Faculty of Life Sciences, EPFL SV-DEC PH-SV, SV 3516 - Station 19, 1015 Lausanne, Switzerland.
authors.forename: Manon
authors.initials: M
authors.lastname: Muller
authors.affiliation: Swiss Federal Institute of Technology Lausanne (EPFL), Faculty of Life Sciences, EPFL SV-DEC PH-SV, SV 3516 - Station 19, 1015 Lausanne, Switzerland.
authors.forename: Peter
authors.initials: P
authors.lastname: Beard
mesh_terms: D032701:Biomimetics; D045744:Cell Line, Tumor; D000229:Dependovirus / Q000254:growth & development*; D006801:Humans; D010011:Osteocytes / Q000821:virology*; D046509:Tissue Culture Techniques