title: Organ repair, hemostasis, and in vivo bonding of medical devices by aqueous solutions of nanoparticles.
abstract: Sutures are traumatic to soft connective tissues, such as liver or lungs. Polymer tissue adhesives require complex in vivo control of polymerization or cross-linking reactions and currently suffer from being toxic, weak, or inefficient within the wet conditions of the body. Herein, we demonstrate using Stöber silica or iron oxide nanoparticles that nanobridging, that is, adhesion by aqueous nanoparticle solutions, can be used in vivo in rats to achieve rapid and strong closure and healing of deep wounds in skin and liver. Nanoparticles were also used to fix polymer membranes to tissues even in the presence of blood flow, such as occurring after liver resection, yielding permanent hemostasis within a minute. Furthermore, medical devices and tissue engineering constructs were fixed to organs such as a beating heart. The simplicity, rapidity, and robustness of nanobridging bode well for clinical applications, surgery, and regenerative medicine.
authors.affiliation: Inserm U1148, LVTS; Université Paris 7, Université Paris 13, Sorbonne Paris Cité, Hôpital Bichat, 46 rue rue H Huchard, 75018 Paris (France); Université Paris 13, Sorbonne Paris Cité, Paris (France).
authors.forename: Anne
authors.initials: A
authors.lastname: Meddahi-Pellé
authors.forename: Aurélie
authors.initials: A
authors.lastname: Legrand
authors.forename: Alba
authors.initials: A
authors.lastname: Marcellan
authors.forename: Liliane
authors.initials: L
authors.lastname: Louedec
authors.forename: Didier
authors.initials: D
authors.lastname: Letourneur
authors.forename: Ludwik
authors.initials: L
authors.lastname: Leibler
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D004867:Equipment Design; D006487:Hemostasis / Q000502:physiology*; D008297:Male; D053758:Nanoparticles / Q000737:chemistry* / Q000378:metabolism*; D051381:Rats; D014014:Tissue Adhesives / Q000737:chemistry*; D014867:Water / Q000737:chemistry; D014945:Wound Healing / Q000502:physiology*
chemical_list: D001672:Biocompatible Materials; D014014:Tissue Adhesives; D014867:Water
keywords: bioadhesives; nanoparticles; scaffolds; surgery; wound repair
references.citation: Clin Appl Thromb Hemost. 2010 Oct;16(5):497-514
references.pmid: 20699255
references.citation: Nanotechnology. 2010 Oct 1;21(39):395103
references.pmid: 20820094
references.citation: Acta Biomater. 2012 Dec;8(12):4365-70
references.pmid: 22885681
references.citation: Transplant Proc. 2003 Aug;35(5):1911-2
references.pmid: 12962845
references.citation: Annu Rev Mater Res. 2011 Aug 1;41:99-132
references.pmid: 22058660
references.citation: Nat Commun. 2013;4:1702
references.pmid: 23591869
references.citation: Emerg Med Clin North Am. 2007 Feb;25(1):73-81
references.pmid: 17400073
references.citation: Chest. 2002 Dec;122(6):2146-9
references.pmid: 12475859
references.citation: Langmuir. 2012 Jan 31;28(4):2200-5
references.pmid: 22224862
references.citation: Eur J Obstet Gynecol Reprod Biol. 2013 Dec;171(2):240-5
references.pmid: 24075447
references.citation: Dig Surg. 2007;24(4):294-9
references.pmid: 17657155
references.citation: Hepatogastroenterology. 2011 May-Jun;58(107-108):922-5
references.pmid: 21830417
references.citation: Adv Healthc Mater. 2013 May;2(5):745-55
references.pmid: 23184616
references.citation: Tissue Eng Part A. 2012 Jan;18(1-2):35-44
references.pmid: 21770864
references.citation: Surgery. 2002 Mar;131(3):270-6
references.pmid: 11894031
references.citation: Ann Surg. 2008 Feb;247(2):399-400; author reply 400
references.pmid: 18216562
references.citation: Transfusion. 2008 Jul;48(7):1502-16
references.pmid: 18422855
references.citation: Carbohydr Polym. 2013 Jul 1;96(1):342-8
references.pmid: 23688490
references.citation: Biomaterials. 2003 Oct;24(22):4091-9
references.pmid: 12834605
references.citation: Ann Surg. 1992 Nov;216(5):524-38
references.pmid: 1444644
references.citation: Dig Surg. 2007;24(4):288-93
references.pmid: 17657154
references.citation: Eur Urol. 2007 Oct;52(4):1156-63
references.pmid: 17467884
references.citation: Proc Natl Acad Sci U S A. 2008 Feb 19;105(7):2307-12
references.pmid: 18287082
references.citation: Contrast Media Mol Imaging. 2012 Jul-Aug;7(4):373-83
references.pmid: 22649043
references.citation: N Engl J Med. 1999 Sep 2;341(10):738-46
references.pmid: 10471461
references.citation: Semin Intervent Radiol. 2012 Jun;29(2):71-80
references.pmid: 23729976
references.citation: Am J Obstet Gynecol. 2010 Jan;202(1):85.e1-9
references.pmid: 20096254
references.citation: Nature. 2007 Jul 19;448(7151):338-41
references.pmid: 17637666
references.citation: J Surg Res. 2011 Dec;171(2):700-8
references.pmid: 22005503
references.citation: Acta Biomed. 2003;74 Suppl 2:21-5
references.pmid: 15055028
references.citation: Transfusion. 2012 Oct;52(10):2243-55
references.pmid: 22612730
references.citation: World J Surg. 1996 Oct;20(8):1107-11; discussion 1111-2
references.pmid: 8798373
references.citation: Nature. 2014 Jan 16;505(7483):382-5
references.pmid: 24336207
references.citation: Biochim Biophys Acta. 2013 Aug;1830(8):4030-9
references.pmid: 23583368
references.citation: Interact Cardiovasc Thorac Surg. 2007 Aug;6(4):529-33
references.pmid: 17669929
references.citation: Mater Sci Eng C Mater Biol Appl. 2014 Feb 1;35:300-6
references.pmid: 24411381
references.citation: Gastrointest Endosc. 2013 Aug;78(2):209-15
references.pmid: 23867370
references.citation: Curr Med Res Opin. 2013 Apr;29(4):387-94
references.pmid: 23339601
references.citation: Dig Surg. 2012;29(1):54-61
references.pmid: 22441621
references.citation: Sci Transl Med. 2014 Jan 8;6(218):218ra6
references.pmid: 24401941
references.citation: Macromol Biosci. 2013 Mar;13(3):271-88
references.pmid: 23225776
references.citation: Biomaterials. 2014 Jan;35(2):711-9
references.pmid: 24140046
references.citation: Am J Emerg Med. 2008 May;26(4):490-6
references.pmid: 18410821
references.citation: Br J Dermatol. 2014 Mar;170(3):739-41
references.pmid: 24125099
references.citation: Biomaterials. 2010 Jan;31(3):420-7
references.pmid: 19811819
references.citation: Biomaterials. 2012 Nov;33(32):7972-83
references.pmid: 22902057
references.citation: Am J Surg. 2001 Aug;182(2 Suppl):15S-20S
references.pmid: 11566472
references.citation: Biomaterials. 2011 Jun;32(16):3988-99
references.pmid: 21392823