title: ANKHD1, a novel component of the Hippo signaling pathway, promotes YAP1 activation and cell cycle progression in prostate cancer cells.
abstract: ANKHD1 is a multiple ankyrin repeat containing protein, recently identified as a novel member of the Hippo signaling pathway. The present study aimed to investigate the role of ANKHD1 in DU145 and LNCaP prostate cancer cells. ANKHD1 and YAP1 were found to be highly expressed in prostate cancer cells, and ANKHD1 silencing decreased cell growth, delayed cell cycle progression at the S phase, and reduced tumor xenograft growth. Moreover, ANKHD1 knockdown downregulated YAP1 expression and activation, and reduced the expression of CCNA2, a YAP1 target gene. These findings indicate that ANKHD1 is a positive regulator of YAP1 and promotes cell growth and cell cycle progression through Cyclin A upregulation.
authors.affiliation: Hematology and Hemotherapy Center, University of Campinas/Hemocentro-Unicamp, Instituto Nacional de Ciência e Tecnologia do Sangue, Campinas, São Paulo, Brazil.
authors.forename: João Agostinho
authors.initials: JA
authors.lastname: Machado-Neto
authors.affiliation: Hematology and Hemotherapy Center, University of Campinas/Hemocentro-Unicamp, Instituto Nacional de Ciência e Tecnologia do Sangue, Campinas, São Paulo, Brazil.
authors.forename: Mariana
authors.initials: M
authors.lastname: Lazarini
authors.affiliation: Hematology and Hemotherapy Center, University of Campinas/Hemocentro-Unicamp, Instituto Nacional de Ciência e Tecnologia do Sangue, Campinas, São Paulo, Brazil; Department of Biological Sciences, Federal University of São Paulo, Diadema, São Paulo, Brazil.
authors.forename: Patricia
authors.initials: P
authors.lastname: Favaro
authors.affiliation: Integrated Center for Childhood Onco-Hematological Investigation, University of Campinas, Campinas, São Paulo, Brazil.
authors.forename: Gilberto Carlos
authors.initials: GC
authors.lastname: Franchi
authors.affiliation: Integrated Center for Childhood Onco-Hematological Investigation, University of Campinas, Campinas, São Paulo, Brazil.
authors.forename: Alexandre Eduardo
authors.initials: AE
authors.lastname: Nowill
authors.affiliation: Hematology and Hemotherapy Center, University of Campinas/Hemocentro-Unicamp, Instituto Nacional de Ciência e Tecnologia do Sangue, Campinas, São Paulo, Brazil.
authors.forename: Sara Teresinha Olalla
authors.initials: ST
authors.lastname: Saad
authors.affiliation: Hematology and Hemotherapy Center, University of Campinas/Hemocentro-Unicamp, Instituto Nacional de Ciência e Tecnologia do Sangue, Campinas, São Paulo, Brazil; Department of Internal Medicine, University of São Paulo at Ribeirão Preto Medical School, Ribeirão Preto, São Paulo, Brazil. Electronic address: ftraina@fmrp.usp.br.
authors.forename: Fabiola
authors.initials: F
authors.lastname: Traina
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D002453:Cell Cycle / Q000235:genetics*; D045744:Cell Line, Tumor; D019925:Cyclin A / Q000235:genetics / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006367:HeLa Cells; D000090683:Hippo Signaling Pathway; D006801:Humans; D020014:K562 Cells; D008297:Male; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D008822:Mice, Transgenic; D010750:Phosphoproteins / Q000235:genetics* / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000502:physiology*; D015398:Signal Transduction / Q000235:genetics; D014157:Transcription Factors; D015533:Transcriptional Activation; D000091102:YAP-Signaling Proteins
chemical_list: C504171:ANKHD1 protein, human; D048868:Adaptor Proteins, Signal Transducing; D019925:Cyclin A; D010750:Phosphoproteins; D016601:RNA-Binding Proteins; D014157:Transcription Factors; D000091102:YAP-Signaling Proteins; C088374:YAP1 protein, human; D017346:Protein Serine-Threonine Kinases
keywords: ANKHD1; Cell cycle; Hippo pathway; MASK; Prostate cancer; YAP1