title: Cystomanamides: structure and biosynthetic pathway of a family of glycosylated lipopeptides from myxobacteria.
abstract: Cystomanamides A-D were isolated as novel natural product scaffolds from Cystobacter fuscus MCy9118, and their structures were established by spectroscopic techniques including 2D NMR, LC-SPE-NMR/-MS, and HR-MS. The cystomanamides contain β-hydroxy amino acids along with 3-amino-9-methyldecanoic acid that is N-glycosylated in cystomanamide C and D. The gene cluster for cystomanamide biosynthesis was identified by gene disruption as PKS/NRPS hybrid incorporating an iso-fatty acid as starter unit and including a reductive amination step at the interface of the PKS and NRPS modules.
authors.affiliation: Department of Microbial Natural Products, Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Helmholtz Centre for Infection Research (HZI) and Pharmaceutical Biotechnology, Saarland University , Campus C2 3, 66123 Saarbrücken, Germany.
authors.forename: Lena
authors.initials: L
authors.lastname: Etzbach
authors.forename: Alberto
authors.initials: A
authors.lastname: Plaza
authors.forename: Ronald
authors.initials: R
authors.lastname: Garcia
authors.forename: Sascha
authors.initials: S
authors.lastname: Baumann
authors.forename: Rolf
authors.initials: R
authors.lastname: Müller
mesh_terms: D000595:Amino Acid Sequence; D024361:Amino Acids, Basic / Q000737:chemistry*; D001688:Biological Products / Q000737:chemistry* / Q000378:metabolism; D053898:Biosynthetic Pathways / Q000235:genetics; D003652:Decanoic Acids / Q000737:chemistry*; D006031:Glycosylation; D055666:Lipopeptides / Q000096:biosynthesis* / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D005810:Multigene Family; D009231:Myxococcales / Q000737:chemistry*
chemical_list: C000591791:3-amino-9-methyldecanoic acid; D024361:Amino Acids, Basic; D001688:Biological Products; D003652:Decanoic Acids; D055666:Lipopeptides