title: Unique RNA 2 sequences of two Brazilian isolates of Pepper ringspot virus, a tobravirus.
abstract: Pepper ringspot virus (PepRSV) is a tobravirus reported only in Brazil. Here, the sequences of the complete RNA 2 segments and the 3' end of the RNA 1 genomic regions of two new isolates from tomato plants were analyzed. The main ORF encodes the CP gene as other tobraviruses and termed ORF 1 of RNA 2. The second ORF was found only in one of the new isolates, although this gene was absent in the type isolate, CAM (collected in the 1960's). Interestingly, this ORF 2 gene did not show any nucleotide and amino acid sequence similarities with known 2b genes of tobraviruses, an essential gene of tobraviruses for nematodes-transmission. The 5'UTR sequence of RNA 2 segment of CAM isolate was previously reported showing two impaired direct repeats; however, the direct-repeats were absent in these new isolates. An additional ORF was predicted upstream of the CP gene. This putative protein possessed a transmembrane domain similar to the ORFN1 of RNA 2 of Tobacco rattle virus SYM isolate, although there was no sequence similarity. This is the first report on the diversity of the RNA 2 sequences of PepRSV.
authors.affiliation: Instituto de Ciências Biológicas, Universidade de Brasília, Brasília, DF, Brazil.
authors.forename: Adriana Ribeiro Silva
authors.initials: AR
authors.lastname: Batista
authors.forename: Cícero
authors.initials: C
authors.lastname: Nicolini
authors.forename: Kelly Barreto
authors.initials: KB
authors.lastname: Rodrigues
authors.forename: Fernando Lucas
authors.initials: FL
authors.lastname: Melo
authors.forename: Raquel Medeiros
authors.initials: RM
authors.lastname: Vasques
authors.forename: Mônica Alves
authors.initials: MA
authors.lastname: de Macêdo
authors.forename: Alice Kazuko
authors.initials: AK
authors.lastname: Inoue-Nagata
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Nagata
mesh_terms: D001938:Brazil; D018551:Solanum lycopersicum / Q000821:virology*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010935:Plant Diseases / Q000821:virology; D010942:Plant Viruses / Q000235:genetics* / Q000302:isolation & purification*; D012328:RNA Viruses / Q000235:genetics* / Q000302:isolation & purification*; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014764:Viral Proteins / Q000235:genetics
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins
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