title: Optimisation of the microencapsulation of tuna oil in gelatin-sodium hexametaphosphate using complex coacervation.
abstract: The microencapsulation of tuna oil in gelatin-sodium hexametaphosphate (SHMP) using complex coacervation was optimised for the stabilisation of omega-3 oils, for use as a functional food ingredient. Firstly, oil stability was optimised by comparing the accelerated stability of tuna oil in the presence of various commercial antioxidants, using a Rancimat™. Then zeta-potential (mV), turbidity and coacervate yield (%) were measured and optimised for complex coacervation. The highest yield of complex coacervate was obtained at pH 4.7 and at a gelatin to SHMP ratio of 15:1. Multi-core microcapsules were formed when the mixed microencapsulation system was cooled to 5 °C at a rate of 12 °C/h. Crosslinking with transglutaminase followed by freeze drying resulted in a dried powder with an encapsulation efficiency of 99.82% and a payload of 52.56%. Some 98.56% of the oil was successfully microencapsulated and accelerated stability using a Rancimat™ showed stability more than double that of non-encapsulated oil.
authors.affiliation: Centre for Chemistry and Biotechnology, Deakin University, Geelong, VIC 3217, Australia.
authors.forename: Bo
authors.initials: B
authors.lastname: Wang
authors.affiliation: School of Applied Sciences, RMIT University, City Campus, Melbourne, VIC 3001, Australia.
authors.forename: Benu
authors.initials: B
authors.lastname: Adhikari
authors.affiliation: Centre for Chemistry and Biotechnology, Deakin University, Geelong, VIC 3217, Australia. Electronic address: colin.barrow@deakin.edu.au.
authors.forename: Colin J
authors.initials: CJ
authors.lastname: Barrow
mesh_terms: D000818:Animals; D002214:Capsules / Q000737:chemistry; D004339:Drug Compounding / Q000379:methods*; D005395:Fish Oils / Q000737:chemistry*; D005780:Gelatin / Q000737:chemistry; D010710:Phosphates / Q000737:chemistry*; D014413:Tuna
chemical_list: D002214:Capsules; D005395:Fish Oils; D010710:Phosphates; D005780:Gelatin; C009285:sodium polymetaphosphate
keywords: Complex coacervation; Gelatin; Microencapsulation; Oxidative stability; Sodium hexametaphosphate; Transglutaminase; Tuna oil