title: MicroRNAs: Novel Mechanism Involved in the Pathogenesis of Microwave Exposure on Rats' Hippocampus.
abstract: Microwave-induced adverse health outcomes have been gaining much attention in recent years. The hippocampus is sensitive and vulnerable to microwave exposure. Studies from our group and others showed that microwave-induced structural and functional injury of hippocampus, accompanied with alteration of gene and protein expression. It has been demonstrated that microRNAs (miRNAs) were involved in the physiological and pathological processes of brain. In this study, the miRNAs expression profiles of microwave-exposed hippocampus were detected by microarray analysis and verified by real-time polymerase chain reaction (PCR). At 7 days after 30 mW/cm(2) microwave exposure, the expression of 12 miRNAs increased, while other 70 miRNAs decreased in rats' hippocampus. However, most of miRNAs restored to normal levels at 14 days after exposure, only two upregulated miRNAs and 14 downregulated miRNAs were detected. Gene transcription, neuroprotection and receptors function related target genes were predicated by miRDB, miRbase and miRanda. Moreover, these differentially expressed miRNAs were involved in brain-related signaling pathways, such as synaptic vesicle cycle, long-term depression, calcium signaling and neurotrophin signaling pathways. In conclusion, we successfully characterized the miRNA profiles in microwave-exposed hippocampus, and that will be helpful to clarify the molecular mechanism and provide potential therapeutic targets.
authors.affiliation: Department of Experimental Pathology, Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing, 100850, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhao
authors.forename: Chengfeng
authors.initials: C
authors.lastname: Sun
authors.forename: Lu
authors.initials: L
authors.lastname: Xiong
authors.forename: Yuefeng
authors.initials: Y
authors.lastname: Yang
authors.forename: Yabing
authors.initials: Y
authors.lastname: Gao
authors.forename: Lifeng
authors.initials: L
authors.lastname: Wang
authors.forename: Hongyan
authors.initials: H
authors.lastname: Zuo
authors.forename: Xinping
authors.initials: X
authors.lastname: Xu
authors.forename: Ji
authors.initials: J
authors.lastname: Dong
authors.forename: Hongmei
authors.initials: H
authors.lastname: Zhou
authors.forename: Ruiyun
authors.initials: R
authors.lastname: Peng
mesh_terms: D000818:Animals; D053263:Gene Regulatory Networks; D006624:Hippocampus / Q000378:metabolism / Q000528:radiation effects*; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008872:Microwaves; D051381:Rats; D017208:Rats, Wistar
chemical_list: D035683:MicroRNAs
references.citation: Mol Neurobiol. 2011 Dec;44(3):223-34
references.pmid: 21739238
references.citation: J Radiat Res. 2008 Mar;49(2):163-70
references.pmid: 18198477
references.citation: Bioelectromagnetics. 2008 Jul;29(5):371-9
references.pmid: 18286519
references.citation: Biochem Biophys Res Commun. 2007 Oct 5;361(4):922-7
references.pmid: 17686463
references.citation: Indian J Biochem Biophys. 2013 Apr;50(2):114-9
references.pmid: 23720885
references.citation: J Biol Chem. 2013 Apr 12;288(15):10742-9
references.pmid: 23400776
references.citation: Nat Rev Neurosci. 2013 Jan;14(1):7-23
references.pmid: 23254191
references.citation: Neurobiol Aging. 2013 Jul;34(7):1902-12
references.pmid: 23428182
references.citation: Biomed Environ Sci. 2012 Apr;25(2):182-8
references.pmid: 22998825
references.citation: Mol Cell Biol. 2012 Feb;32(3):619-32
references.pmid: 22144581
references.citation: Int J Radiat Biol. 2013 Dec;89(12):1100-7
references.pmid: 23786183
references.citation: Mol Pharmacol. 2007 Dec;72(6):1476-83
references.pmid: 17848599
references.citation: Proc Natl Acad Sci U S A. 2011 Feb 22;108(8):3270-5
references.pmid: 21289278
references.citation: Indian J Exp Biol. 2013 Mar;51(3):187-200
references.pmid: 23678539
references.citation: Brain Dev. 2011 Nov;33(10):856-65
references.pmid: 21908116
references.citation: Neuroreport. 2007 Feb 12;18(3):297-300
references.pmid: 17314675
references.citation: Int J Radiat Biol. 2012 Nov;88(11):799-805
references.pmid: 22788526
references.citation: Exp Neurol. 2012 Jun;235(2):419-26
references.pmid: 22085592
references.citation: Glia. 2011 Oct;59(10):1472-88
references.pmid: 21656854
references.citation: Trends Neurosci. 2012 May;35(5):325-34
references.pmid: 22436491
references.citation: Appl Biochem Biotechnol. 2012 Jan;166(2):379-88
references.pmid: 22134878
references.citation: Synapse. 2009 Nov;63(11):1010-6
references.pmid: 19603498
references.citation: Trends Cell Biol. 2013 Jan;23(1):30-6
references.pmid: 23026030
references.citation: Steroids. 2006 Nov;71(11-12):966-78
references.pmid: 16949628
references.citation: Bioelectromagnetics. 2007 Dec;28(8):599-607
references.pmid: 17620299
references.citation: Stroke. 2013 Jun;44(6 Suppl 1):S17-9
references.pmid: 23709715
references.citation: Cancer Res. 2013 Jan 15;73(2):473-7
references.pmid: 23316035
references.citation: Neurosci Lett. 2006 May 8;398(3):253-7
references.pmid: 16443327
references.citation: J Neurosci. 2010 Sep 1;30(35):11815-25
references.pmid: 20810901
references.citation: Electromagn Biol Med. 2013 Sep;32(3):273-80
references.pmid: 23320534