title: Recovery from stress - a cell cycle perspective.
abstract: We develop a Boolean model to explore the dynamical behaviour of budding yeast in response to osmotic and pheromone stress. Our model predicts that osmotic stress halts the cell cycle progression in either of four possible arrest points. The state of the cell at the onset of the stress dictates which arrest point is finally reached. According to our study and consistent with biological data, these cells can return to the cell cycle after removal of the stress. Moreover, the Boolean model illustrates how osmotic stress alters the state transitions of the cell. Furthermore, we investigate the influence of a particular pheromone based method for the synchronisation of the cell cycles in a population of cells. We show this technique is not a suitable method to study one of the arrest points under osmotic stress. Finally, we discuss how an osmotic stress can cause some of the so calledfrozencells to divide. In this case the stress can move these cells to the cell cycle trajectory, such that they will replicate again.
authors.affiliation: Institute for Complex Systems and Mathematical Biology, SUPA, University of Aberdeen, Aberdeen, AB24 3UE, UK.
authors.forename: Elahe
authors.initials: E
authors.lastname: Radmaneshfar
authors.affiliation: Institute for Complex Systems and Mathematical Biology, SUPA, University of Aberdeen, Aberdeen, AB24 3UE, UK.
authors.forename: Marco
authors.initials: M
authors.lastname: Thiel
keywords: Boolean network; alpha factor; cell cycle; osmotic stress; state transition; stress response
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