title: Coordination of transposon expression with DNA replication in the targeting of telomeric retrotransposons in Drosophila.
abstract: In Drosophila, a group of retrotransposons is mobilized exclusively to telomeres in a sequence-independent manner. How they target chromosome ends is not understood. Here, we focused on the telomeric element HeT-A and characterized the cell cycle expression and cytological distribution of its protein and RNA products. We determined the timing of telomere replication by creating a single lacO-marked telomere and provide evidence suggesting that transposon expression and recruitment to telomeres is linked to telomere replication. The HeT-A-encoded ORF1p protein is expressed predominantly in S phase, particularly in early S phase. Orf1p binds HeT-A transcripts and forms spherical structures at telomeres undergoing DNA replication. HeT-A sphere formation requires Verrocchio, a putative homolog of the conserved Stn1 telomeric protein. Our results suggest that coupling of telomere elongation and telomere replication is a universal feature, and raise the possibility that transposon recruitment to Drosophila telomeres is mechanistically related to telomerase recruitment in other organisms. Our study also supports a co-adaptive relationship between the Drosophila host and HeT-A mobile elements.
authors.affiliation: Laboratory of Biochemistry and Molecular Biology, National Cancer Institute (NCI) NIH, Bethesda, MD, USA.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Laboratory of Biochemistry and Molecular Biology, National Cancer Institute (NCI) NIH, Bethesda, MD, USA.
authors.forename: Michelle
authors.initials: M
authors.lastname: Beaucher
authors.affiliation: Laboratory of Biochemistry and Molecular Biology, National Cancer Institute (NCI) NIH, Bethesda, MD, USA.
authors.forename: Yan
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: Laboratory of Biochemistry and Molecular Biology, National Cancer Institute (NCI) NIH, Bethesda, MD, USA rongy@mail.nih.gov.
authors.forename: Yikang S
authors.initials: YS
authors.lastname: Rong
mesh_terms: D000818:Animals; D004261:DNA Replication / Q000235:genetics / Q000502:physiology*; D004251:DNA Transposable Elements / Q000235:genetics*; D004330:Drosophila / Q000235:genetics; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D015683:Gene Products, gag / Q000235:genetics / Q000378:metabolism; D018626:Retroelements / Q000235:genetics*; D016615:Telomere / Q000235:genetics*
chemical_list: D004251:DNA Transposable Elements; D029721:Drosophila Proteins; D015683:Gene Products, gag; C103996:HeT-A-gag protein, Drosophila; C117553:I-element-gag protein, Drosophila; D018626:Retroelements
keywords: CST complex; Drosophila telomere; telomere replication; telomeric transposon
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