title: Expression of mitochondrial superoxide dismutase in polymorphonuclear leukocytes from patients with type 1 diabetes with and without microvascular complications.
abstract: BACKGROUND
  One of the causes of impaired antioxidant response in patients with type 1 diabetes might be decreased expression of mitochondrial manganese superoxide dismutase (MnSOD).
  
  
  OBJECTIVE
  The aim of this study was to evaluate the expression of MnSOD on transcript and protein levels in polymorphonuclear leukocytes (PMNLs) from patients with type 1 diabetes and analyze its association with microvascular complications.
  
  
  METHODS
  The MnSOD expression was assessed in PMNLs from 46 patients with type 1 diabetes and 12 age- and sex -matched healthy subjects. The study group was divided into 2 subgroups: with and without microvascular complications. The MnSOD expression on the transcript level was evaluated by real -time quantitative polymerase chain reaction, while that on the protein level by Western blot analysis.
  
  
  RESULTS
  A significant increase in the MnSOD transcript level was observed in all patients with diabetes with and without microvascular complications (P = 0.01, P = 0.02, respectively). The MnSOD protein level was higher in patients without microvascular complications compared with those with complications and the control group (P = 0.05, P = 0.03, respectively). The MnSOD expression was positively correlated with fasting plasma glucose and total cholesterol levels both at the transcript level (r = 0.4, P <0.05 for both correlations) and at the protein level (r = 0.3 and r = 0.4, respectively, P <0.05).
  
  
  CONCLUSIONS
  Although an increased MnSOD transcript level in patients with type 1 diabetes suggests enhanced antioxidant mobilization in all diabetic patients, decreased levels of the MnSOD protein in PMNLs from patients with microvascular complications compared with those without complications indicates that patients with microvascular complications may have impaired antioxidant response.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Wegner
authors.forename: Agnieszka A
authors.initials: AA
authors.lastname: Rawłuszko-Wieczorek
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Araszkiewicz
authors.forename: Maria
authors.initials: M
authors.lastname: Pioruńska-Stolzmann
authors.forename: Dorota
authors.initials: D
authors.lastname: Zozulińska-Ziółkiewicz
authors.forename: Bogna
authors.initials: B
authors.lastname: Wierusz-Wysocka
authors.forename: Paweł P
authors.initials: PP
authors.lastname: Jagodziński
mesh_terms: D003922:Diabetes Mellitus, Type 1 / Q000201:enzymology*; D003925:Diabetic Angiopathies / Q000201:enzymology*; D005260:Female; D006801:Humans; D008297:Male; D008928:Mitochondria / Q000378:metabolism*; D009504:Neutrophils / Q000201:enzymology*; D013482:Superoxide Dismutase / Q000378:metabolism*
chemical_list: D013482:Superoxide Dismutase