title: Direct intrawound administration of dimethylsulphoxide relieves acute pain in rats.
abstract: Wounds associated with injuries such as burns can produce moderate to severe pain. Besides causing distress to the patient, unrelieved pain could delay healing owing to stress-related problems. Thus, pain needs to be treated as early as possible after injury. It was hypothesised that local treatment of wounds with appropriate analgesic drugs could attenuate pain. HOE 140, a bradykinin receptor antagonist, reduced acute inflammatory pain in rats after intrawound administration. In this study, the analgesic effect of dimethylsulphoxide (DMSO) was investigated in a similar hind-paw incision model in rats. An extremely small quantity (10 µl) of 100% DMSO was administered into the incision site just before closure of the wound. It persistently attenuated guarding behaviour in rats over a period of 3 days without affecting thermal hyperalgesia or allodynia. Accumulated evidence indicates that guarding is equivalent to pain at rest in humans. The possible mechanisms of the analgesic effect could be inhibition of C group of peripheral nerve fibres or even free radical scavenging. Healing of the wound was found to be normal at the end of the study period. In conclusion, DMSO could be useful in the treatment of acute pain resulting from tissue injuries such as burns.
authors.affiliation: Department of Anatomy, All India Institute of Medical Sciences, New Delhi, India.
authors.forename: Mayank
authors.initials: M
authors.lastname: Gautam
authors.affiliation: Department of Anatomy, All India Institute of Medical Sciences, New Delhi, India.
authors.forename: Pranav
authors.initials: P
authors.lastname: Prasoon
authors.affiliation: Department of Anatomy, All India Institute of Medical Sciences, New Delhi, India.
authors.forename: Rahul
authors.initials: R
authors.lastname: Kumar
authors.affiliation: Department of Anatomy, All India Institute of Medical Sciences, New Delhi, India.
authors.forename: Anurag
authors.initials: A
authors.lastname: Singh
authors.affiliation: Department of Anatomy, All India Institute of Medical Sciences, New Delhi, India.
authors.forename: Prawal
authors.initials: P
authors.lastname: Shrimal
authors.affiliation: Department of Anatomy, All India Institute of Medical Sciences, New Delhi, India.
authors.forename: Subrata B
authors.initials: SB
authors.lastname: Ray
mesh_terms: D059787:Acute Pain / Q000188:drug therapy* / Q000209:etiology; D000818:Animals; D004121:Dimethyl Sulfoxide / Q000008:administration & dosage*; D004195:Disease Models, Animal; D016166:Free Radical Scavengers / Q000008:administration & dosage; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D007507:Therapeutic Irrigation / Q000379:methods*; D014945:Wound Healing / Q000187:drug effects*; D014947:Wounds and Injuries / Q000150:complications* / Q000188:drug therapy
chemical_list: D016166:Free Radical Scavengers; D004121:Dimethyl Sulfoxide
keywords: DMSO; Guarding; Inflammation tissue injury; Treatment
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