title: Rapid adaptive optical recovery of optimal resolution over large volumes.
abstract: Using a descanned, laser-induced guide star and direct wavefront sensing, we demonstrate adaptive correction of complex optical aberrations at high numerical aperture (NA) and a 14-ms update rate. This correction permits us to compensate for the rapid spatial variation in aberration often encountered in biological specimens and to recover diffraction-limited imaging over large volumes (>240 mm per side). We applied this to image fine neuronal processes and subcellular dynamics within the zebrafish brain.
authors.affiliation: Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA.
authors.forename: Kai
authors.initials: K
authors.lastname: Wang
authors.affiliation: Coleman Technologies, Inc., Newtown Square, Pennsylvania, USA.
authors.forename: Daniel E
authors.initials: DE
authors.lastname: Milkie
authors.affiliation: Division of Biology, California Institute of Technology, Pasadena, California, USA.
authors.forename: Ankur
authors.initials: A
authors.lastname: Saxena
authors.affiliation: Institute of Neuronal Cell Biology, Technische Universität München, Munich, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Engerer
authors.affiliation: 1] Institute of Neuronal Cell Biology, Technische Universität München, Munich, Germany. [2] Munich Center for Systems Neurology, Munich, Germany. [3] German Center for Neurodegenerative Diseases, Munich, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Misgeld
authors.affiliation: Division of Biology, California Institute of Technology, Pasadena, California, USA.
authors.forename: Marianne E
authors.initials: ME
authors.lastname: Bronner
authors.affiliation: 1] Department of Cellular Biology and Anatomy, Georgia Regents University, Augusta, Georgia, USA. [2].
authors.forename: Jeff
authors.initials: J
authors.lastname: Mumm
authors.affiliation: 1] Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA. [2].
authors.forename: Eric
authors.initials: E
authors.lastname: Betzig
mesh_terms: D000818:Animals; D002460:Cell Line; D018613:Microscopy, Confocal / Q000295:instrumentation* / Q000706:statistics & numerical data*; D055095:Optics and Photonics / Q000295:instrumentation* / Q000592:standards; D013997:Time Factors; D015027:Zebrafish
references.citation: Nat Methods. 2011 May;8(5):417-23
references.pmid: 21378978
references.citation: Cell. 2012 Dec 7;151(6):1370-85
references.pmid: 23217717
references.citation: J Neurosci. 2012 Nov 14;32(46):16203-12
references.pmid: 23152604
references.citation: Science. 2008 Nov 14;322(5904):1065-9
references.pmid: 18845710
references.citation: Opt Lett. 2009 Aug 15;34(16):2495-7
references.pmid: 19684827
references.citation: Nat Methods. 2013 May;10(5):413-20
references.pmid: 23524393
references.citation: Nat Methods. 2010 Feb;7(2):141-7
references.pmid: 20037592
references.citation: Nature. 2012 May 09;485(7399):471-7
references.pmid: 22622571
references.citation: Curr Opin Genet Dev. 2011 Oct;21(5):558-65
references.pmid: 21862314
references.citation: Curr Opin Genet Dev. 2011 Oct;21(5):566-72
references.pmid: 21963791
references.citation: Opt Express. 2004 Dec 27;12(26):6540-52
references.pmid: 19488305
references.citation: Philos Trans A Math Phys Eng Sci. 2007 Dec 15;365(1861):2829-43
references.pmid: 17855218
references.citation: Neuron. 2007 May 24;54(4):535-45
references.pmid: 17521567
references.citation: Development. 2012 Sep;139(17):3242-7
references.pmid: 22872089
references.citation: Mech Dev. 2003 Feb;120(2):211-8
references.pmid: 12559493
references.citation: Dev Dyn. 1995 Jul;203(3):253-310
references.pmid: 8589427
references.citation: J Opt Soc Am A Opt Image Sci Vis. 2001 Mar;18(3):497-506
references.pmid: 11265680
references.citation: Opt Lett. 2011 Mar 15;36(6):870-2
references.pmid: 21403712
references.citation: BMC Biol. 2012 Nov 30;10:93
references.pmid: 23198762
references.citation: Opt Lett. 2011 Sep 1;36(17):3389-91
references.pmid: 21886220
references.citation: Opt Lett. 2011 Nov 1;36(21):4206-8
references.pmid: 22048366
references.citation: Development. 2005 Sep;132(17):3977-88
references.pmid: 16049113
references.citation: Biomed Opt Express. 2011 Nov 1;2(11):3135-49
references.pmid: 22076274
references.citation: Opt Lett. 2004 Dec 1;29(23):2707-9
references.pmid: 15605479
references.citation: Development. 2006 Dec;133(23):4619-30
references.pmid: 17065232
references.citation: Dev Dyn. 2005 Feb;232(2):359-68
references.pmid: 15614774
references.citation: Opt Lett. 2011 Apr 1;36(7):1062-4
references.pmid: 21478983