title: Silencing the radicals improves Click Chemistry.
abstract: Modern fluorescence imaging microscopy in living and fixed material makes use of fluorescent probes to label targeted entities. Common labelling approaches include classical immunocytochemistry, expression of chimerically tagged fluorescent protein domains, and chemical affinity-binding or covalent labelling. Of these methods, the so-called "Click Chemistry", is emerging as one of the most influential labelling chemistries introduced in recent times, offering enormous utility for bio-orthoganol attachment of fluorescent probes to biological target entities. In this issue of Biotechnology Journal, Löschberger, Niehörster and Sauer report "ClickOx", a Click Chemistry protocol that uses an enzymatic oxygen scavenger system to reduce concurrent ROS-associated damage during Click labeling.
authors.affiliation: Institut Pasteur, Imagopole, Plateforme d'imagerie dynamique, Paris, France; INSERM U786, Paris, France.
authors.forename: Régis
authors.initials: R
authors.lastname: Tournebize
authors.forename: Joe
authors.initials: J
authors.lastname: Dragavon
authors.forename: Andrew D
authors.initials: AD
authors.lastname: Miller
authors.forename: Spencer
authors.initials: S
authors.lastname: Shorte
mesh_terms: D000818:Animals; D022082:Cellular Structures / Q000648:ultrastructure*; D057930:Click Chemistry / Q000379:methods*; D049452:Green Fluorescent Proteins / Q000737:chemistry*; D006801:Humans; D057054:Molecular Imaging / Q000379:methods*
chemical_list: D049452:Green Fluorescent Proteins