title: Effect of cortisol on caspases in the co-cultured C2C12 and 3 T3-L1 cells.
abstract: The present study was carried out to understand the effect of cortisol on caspase expression in the C2C12 and 3 T3-L1 cells under co-culture system. Cells were co-cultured by using transwell inserts with a 0.4-μm porous membrane to separate C2C12 and 3 T3-L1 preadipocyte cells. Each cell type was grown independently on the transwell plates. Following cell differentiation, inserts containing 3 T3-L1 cells were transferred to C2C12 plates and inserts containing C2C12 cells were transferred to 3 T3-L1 plates. A total of 10 μg/μl of cortisol was added to the medium. Following treatment of cortisol for 3 days, the cells in the lower well were harvested for analysis. Caspases such as caspase 3, caspase 7, and caspase 9 were selected for the analysis. qRT-PCR results indicated the significant increase in the mRNA expression of caspase 3, caspase 7, and caspase 9. Caspase 3, 7, and 9 activities were also increased in the mono- and co-cultured C2C12 and 3 T3-L1 cells. In addition, confocal microscopical investigation indicated that cortisol increases caspase expressions in the mono- and co-cultured C2C12 and 3 T3-L1 cells. Taking all these together, we concluded that the co-culture system reflects the exact effect of cortisol on caspase expression, which is quite distinct from one dimensional mono-cultured experiments.
authors.affiliation: Department of Food Science and Nutrition, Catholic University of Daegu, Daegu, South Korea, frenzram80@gmail.com.
authors.forename: Pandurangan
authors.initials: P
authors.lastname: Muthuraman
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology / Q000201:enzymology*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D020169:Caspases / Q000096:biosynthesis*; D018920:Coculture Techniques; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D006854:Hydrocortisone / Q000494:pharmacology*; D051379:Mice
chemical_list: D000893:Anti-Inflammatory Agents; D020169:Caspases; D006854:Hydrocortisone