title: Effects of supplemental chromium propionate and rumen-protected amino acids on nutrient metabolism, neutrophil activation, and adipocyte size in dairy cows during peak lactation.
abstract: The objective of this study was to evaluate effects of chromium propionate (CrPr), rumen-protected lysine and methionine (RPLM), or both on metabolism, neutrophil function, and adipocyte size in lactating dairy cows (38 ± 15 d in milk). Forty-eight individually fed Holstein cows (21 primiparous, 27 multiparous) were stratified by calving date in 12 blocks and randomly assigned to 1 of 4 treatments within block. Treatments were control, CrPr (8 mg/d of Cr, KemTRACE brand chromium propionate 0.04%, Kemin Industries Inc., Des Moines, IA), RPLM (10 g/d lysine and 5 g/d methionine intestinally available, from LysiPEARL and MetiPEARL, Kemin Industries Inc.), or CrPr plus RPLM. Treatments were fed for 35 d; blood plasma samples were collected ond 21 and 35 of treatment, and blood neutrophils were isolated from 24 cows for analysis of tumor necrosis factor α (TNFα) and interleukin 1β (IL-1β) transcript abundance in the basal state and after 12h of lipopolysaccharide (LPS) activation. Tailhead subcutaneous adipose tissue samples were collected ond 35 for measurement of adipocyte size. Plasma glucose, nonesterified fatty acids, and glucagon concentrations were unaffected by treatments, whereas plasma insulin concentration was increased by RPLM. Basal TNFα transcript abundance in neutrophils was not affected by treatment, but basal IL-1β transcript abundance was decreased by RPLM and tended to be increased by CrPr. After LPS activation, CrPr increased neutrophil TNFα transcript abundance. In addition, RPLM×parity interactions were detected for both TNFα and IL-1β abundance after LPS activation, reflecting enhanced responses in primiparous cows and attenuated responses in multiparous cows supplemented with RPLM. Adipocyte size was not affected by treatment. Supplemental CrPr and RPLM had minimal effects on metabolism when fed for 35 d near peak lactation but may modulate innate immune function in lactating dairy cows.
authors.affiliation: Department of Animal Sciences and Industry, Kansas State University, Manhattan 66506.
authors.forename: K
authors.initials: K
authors.lastname: Yuan
authors.affiliation: Department of Animal Sciences and Industry, Kansas State University, Manhattan 66506.
authors.forename: C F
authors.initials: CF
authors.lastname: Vargas-Rodriguez
authors.affiliation: Department of Animal Sciences and Industry, Kansas State University, Manhattan 66506.
authors.forename: L K
authors.initials: LK
authors.lastname: Mamedova
authors.affiliation: Department of Animal Sciences and Industry, Kansas State University, Manhattan 66506.
authors.forename: M B
authors.initials: MB
authors.lastname: Muckey
authors.affiliation: Department of Animal Sciences and Industry, Kansas State University, Manhattan 66506.
authors.forename: M A
authors.initials: MA
authors.lastname: Vaughn
authors.affiliation: Department of Animal Sciences and Industry, Kansas State University, Manhattan 66506.
authors.forename: D D
authors.initials: DD
authors.lastname: Burnett
authors.affiliation: Department of Animal Sciences and Industry, Kansas State University, Manhattan 66506.
authors.forename: J M
authors.initials: JM
authors.lastname: Gonzalez
authors.affiliation: Department of Animal Sciences and Industry, Kansas State University, Manhattan 66506.
authors.forename: E C
authors.initials: EC
authors.lastname: Titgemeyer
authors.affiliation: Kemin Industries, Des Moines, IA 50317.
authors.forename: K E
authors.initials: KE
authors.lastname: Griswold
authors.affiliation: Department of Animal Sciences and Industry, Kansas State University, Manhattan 66506. Electronic address: bbradfor@ksu.edu.
authors.forename: B J
authors.initials: BJ
authors.lastname: Bradford
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000187:drug effects*; D052242:Adiponectin / Q000097:blood; D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D002417:Cattle; D004032:Diet / Q000662:veterinary; D019587:Dietary Supplements; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005260:Female; D005934:Glucagon / Q000097:blood; D007328:Insulin / Q000097:blood; D053583:Interleukin-1beta / Q000378:metabolism; D007774:Lactation; D020738:Leptin / Q000097:blood; D008239:Lysine / Q000008:administration & dosage* / Q000097:blood; D008715:Methionine / Q000008:administration & dosage* / Q000097:blood; D018375:Neutrophil Activation / Q000187:drug effects*; D011422:Propionates / Q000008:administration & dosage*; D012417:Rumen / Q000187:drug effects* / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D052242:Adiponectin; D001786:Blood Glucose; D005230:Fatty Acids, Nonesterified; D007328:Insulin; D053583:Interleukin-1beta; D020738:Leptin; D011422:Propionates; D014409:Tumor Necrosis Factor-alpha; D005934:Glucagon; D008715:Methionine; C029658:propionic acid; D008239:Lysine
keywords: chromium; immunity; lysine; methionine