title: Analytical evaluation of a high-throughput enzyme-linked immunosorbent assay for acrylamide determination in fried foods.
abstract: The analytical performance and evaluation of a kit-based ELISA for the determination of acrylamide in fried potato and corn chip samples are described. The sample homogenate is subjected to clean-up using SPE, followed by analyte derivatization and ELISA detection. Accuracy, precision and linearity of the ELISA procedure have been validated using spiked samples. Analytical recovery ranged from 91.8% to 96.0% with coefficients of variation below 15%. Good linearity over a wide range of dilution and minimal assay drift was observed within a microtiter plate. IC50 value of the calibration curve was 110 ng/mL, with the limit of detection about 5 ng/mL and dynamic range from 10 to 1000 ng/mL. The high specificity of the ELISA was demonstrated by cross-reactivity study using 11 potential cross-reactants. A good correlation between the results obtained from the ELISA and GC-MS within the concentration range 120-1500 μg/kg was found in the chip samples (r=0.992, n=120). The data demonstrate that the evaluated and validated ELISA has a potential utility in a quick, simple and reliable acrylamide screening analysis for the medium- and large-sized food companies, as well as for residue laboratories and the food industry dealing with improving the chemical safety of foods available to the consumer.
authors.affiliation: Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic.
authors.forename: Milan
authors.initials: M
authors.lastname: Franek
authors.affiliation: Abraxis LLC, 54 Steamwhistle Drive, Warminster, PA 18974, USA.
authors.forename: Daniel
authors.initials: D
authors.lastname: Rubio
authors.affiliation: Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic.
authors.forename: Iva
authors.initials: I
authors.lastname: Diblikova
authors.affiliation: Abraxis LLC, 54 Steamwhistle Drive, Warminster, PA 18974, USA.
authors.forename: Fernando
authors.initials: F
authors.lastname: Rubio
mesh_terms: D020106:Acrylamide / Q000032:analysis*; D003296:Cooking / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D006358:Hot Temperature; D015203:Reproducibility of Results; D011198:Solanum tuberosum / Q000737:chemistry; D003313:Zea mays / Q000737:chemistry
chemical_list: D020106:Acrylamide
keywords: Acrylamide; Chip samples; ELISA kit; Food industry; Screening method; Validation