title: DNA barcoding of five common stored-product pest species of genus Cryptolestes (Coleoptera: Laemophloeidae).
abstract: Several species of the genus Cryptolestes Ganglbauer, 1899 (Coleoptera: Laemophloeidae) are commonly found in stored products. In this study, five species of Cryptolestes, with almost worldwide distribution, were obtained from laboratories in China, Czech Republic and the USA: Cryptolestes ferrugineus (Stephens, 1831), Cryptolestes pusillus (Schönherr, 1817), Cryptolestes turcicus (Grouvelle, 1876), Cryptolestes pusilloides (Steel & Howe, 1952) and Cryptolestes capensis (Waltl, 1834). Molecular identification based on a 658 bp fragment from the mitochondrial DNA cytochrome c oxidase subunit I (COI) was adopted to overcome some problems of morphological identification of Cryptolestes species. The utility of COI sequences as DNA barcodes in discriminating the five Cryptolestes species was evaluated on adults and larvae by analysing Kimura 2-parameter distances, phylogenetic tree and haplotype networks. The results showed that molecular approaches based on DNA barcodes were able to accurately identify these species. This is the first study using DNA barcoding to identify Cryptolestes species and the gathered DNA sequences will complement the biological barcode database.
authors.affiliation: Department of Entomology, College of Agriculture and Biotechnology,China Agricultural University,No. 2 Yuanmingyuan West Road, Beijing,China.
authors.forename: Y J
authors.initials: YJ
authors.lastname: Wang
authors.affiliation: Department of Entomology, College of Agriculture and Biotechnology,China Agricultural University,No. 2 Yuanmingyuan West Road, Beijing,China.
authors.forename: Z H
authors.initials: ZH
authors.lastname: Li
authors.affiliation: Institute of Animal and Plant Quarantine,Chinese Academy of Inspection and Quarantine,Beijing,China.
authors.forename: S F
authors.initials: SF
authors.lastname: Zhang
authors.affiliation: Department of Zoology, Faculty of Science,Charles University in Prague,Viničná 7, Prague,Czech Republic.
authors.forename: Z
authors.initials: Z
authors.lastname: Varadínová
authors.affiliation: Department of Entomology, College of Agriculture and Biotechnology,China Agricultural University,No. 2 Yuanmingyuan West Road, Beijing,China.
authors.forename: F
authors.initials: F
authors.lastname: Jiang
authors.affiliation: Department of Pest Control of Stored Products and Food Safety,Crop Research Institute,Drnovská 507, Prague,Czech Republic.
authors.forename: Z
authors.initials: Z
authors.lastname: Kučerová
authors.affiliation: Department of Pest Control of Stored Products and Food Safety,Crop Research Institute,Drnovská 507, Prague,Czech Republic.
authors.forename: V
authors.initials: V
authors.lastname: Stejskal
authors.affiliation: Department of Entomology and Plant Pathology,Oklahoma State University,Stillwater, OK,USA.
authors.forename: G
authors.initials: G
authors.lastname: Opit
authors.affiliation: Academy of State Administration of Grain,No. 11 Baiwanzhuang Street, Beijing,China.
authors.forename: Y
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Academy of State Administration of Grain,No. 11 Baiwanzhuang Street, Beijing,China.
authors.forename: F J
authors.initials: FJ
authors.lastname: Li
mesh_terms: D000818:Animals; D002965:Classification / Q000379:methods; D001517:Coleoptera / Q000235:genetics*; D058893:DNA Barcoding, Taxonomic; D004272:DNA, Mitochondrial / Q000737:chemistry; D014644:Genetic Variation; D006239:Haplotypes; D010802:Phylogeny; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D004272:DNA, Mitochondrial