title: Silver nanoparticle/bacterial cellulose gel membranes for antibacterial wound dressing: investigation in vitro and in vivo.
abstract: Bacterial cellulose (BC) has attracted increasing attention as a novel wound dressing material, but its antimicrobial activity, which is one of the critical skin-barrier functions in wound healing, is not sufficient for use in practical applications. To overcome such a deficiency, silver nanoparticles were generated and self-assembled on the surface of BC nanofibers, forming a stable and evenly distributed Ag nanoparticle coated BC nanofiber (AgNP-BC). The performance of AgNP-BC was systematically studied in terms of antibacterial activities, cytocompatibility and effects on wound healing. The results showed that AgNP-BC exhibited significant antibacterial activity against Staphylococcus aureus. Moreover, AgNP-BC allowed attachment, and growth of rat fibroblasts with low cytotoxicity emerged. Based on these advantages, AgNP-BC samples were applied in a second-degree rat wound model. Wound flora showed a significant reduction during the healing. The fresh epidermal and dermis thicknesses with AgNP-BC samples were 111 and 855 µm respectively, higher than 74 and 619 µm for BC groups and 57 and 473 µm for untreated control wounds. The results demonstrated that AgNP-BC could reduce inflammation and promote scald wound healing.
authors.affiliation: School of Materials Science and Engineering, University of Science and Technology Beijing, Beijing 100083, People's Republic of China.
authors.forename: Jian
authors.initials: J
authors.lastname: Wu
authors.forename: Yudong
authors.initials: Y
authors.lastname: Zheng
authors.forename: Xiaoxiao
authors.initials: X
authors.lastname: Wen
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Lin
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Chen
authors.forename: Zhigu
authors.initials: Z
authors.lastname: Wu
mesh_terms: D000287:Administration, Topical; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000737:chemistry; D047070:Bandages, Hydrocolloid; D002056:Burns / Q000175:diagnosis / Q000382:microbiology / Q000628:therapy*; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D003131:Combined Modality Therapy; D015195:Drug Design; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D005782:Gels / Q000737:chemistry; D008297:Male; D008567:Membranes, Artificial; D053768:Metal Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D053769:Nanocapsules / Q000008:administration & dosage / Q000737:chemistry / Q000648:ultrastructure; D051381:Rats; D017208:Rats, Wistar; D012834:Silver / Q000008:administration & dosage* / Q000737:chemistry; D013211:Staphylococcus aureus / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D016896:Treatment Outcome
chemical_list: D000900:Anti-Bacterial Agents; D005782:Gels; D008567:Membranes, Artificial; D053769:Nanocapsules; D012834:Silver