title: Development of a FTIR-ATR based model for typing clinically relevant Acinetobacter baumannii clones belonging to ST98, ST103, ST208 and ST218.
abstract: In this work we developed and validated a FTIR-ATR (Fourier transform infrared spectroscopy with attenuated total reflectance) based model for typing Acinetobacter baumannii clinical isolates belonging to ST98, ST208 and ST218 included into the worldwide spread clonal complex (CC) 92 and ST103. FTIR-ATR spectra of seventy-seven previously characterized isolates (Multi Locus Sequence Type-MLST, Pulsed-Field Gel Electrophoresis-PFGE and carbapenem-hydrolyzing class D β-lactamase-CHDL) were acquired and modeled by partial least squares discriminant analysis (PLSDA). The model was tested and successfully validated with a diverse collection of isolates (n=148) recovered from different countries and periods of time belonging to modeled and non-modeled STs.
authors.affiliation: REQUIMTE, Microbiology Laboratory, Department of Biological Sciences, Faculty of Pharmacy, University of Porto, R. Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal. Electronic address: ccssousa@gmail.com.
authors.forename: Clara
authors.initials: C
authors.lastname: Sousa
authors.affiliation: REQUIMTE, Microbiology Laboratory, Department of Biological Sciences, Faculty of Pharmacy, University of Porto, R. Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal.
authors.forename: Liliana
authors.initials: L
authors.lastname: Silva
authors.affiliation: REQUIMTE, Microbiology Laboratory, Department of Biological Sciences, Faculty of Pharmacy, University of Porto, R. Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal.
authors.forename: Filipa
authors.initials: F
authors.lastname: Grosso
authors.affiliation: REQUIMTE, Applied Chemistry Laboratory, Department of Biological Sciences, Faculty of Pharmacy, University of Porto, R. Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal; Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, University of Lisbon, Av. Prof. Gama Pinto, 1649-003 Lisboa, Portugal.
authors.forename: João
authors.initials: J
authors.lastname: Lopes
authors.affiliation: REQUIMTE, Microbiology Laboratory, Department of Biological Sciences, Faculty of Pharmacy, University of Porto, R. Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal.
authors.forename: Luísa
authors.initials: L
authors.lastname: Peixe
mesh_terms: D000151:Acinetobacter Infections / Q000175:diagnosis / Q000382:microbiology; D040981:Acinetobacter baumannii / Q000145:classification* / Q000302:isolation & purification / Q000378:metabolism; D016002:Discriminant Analysis; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D008954:Models, Biological; D058885:Multilocus Sequence Typing; D012703:Serotyping; D017550:Spectroscopy, Fourier Transform Infrared
keywords: Bacteria; Chemometry; Discriminant analysis; Epidemiology; FTIR; Spectroscopy; Typing