title: Cellulase-assisted extraction and antioxidant activity of polysaccharides from Rhizoma imperata.
abstract: In this study, the cellulase-assisted extraction and antioxidant activity of the polysaccharides from Rhizoma imperata were investigated. To improve the yield of R. Imperata polysaccharides (RPs), the extraction conditions were optimized as follows: time, 69.48 min; temperature, 45.36°C; pH, 4.58; cellulase amount, 1,200 U/g. Under these optimum conditions, the yield of RPs reached 0.67% (w/w), and was higher than that of the traditionally aqueous extraction method. The sugar content in the RPs product reached up to 93.25% (w/w). The RPs product has high antioxidant activity including hydroxyl radical scavenging activity and 2,2-diphenyl-β-picrylhydrazyl radical scavenging activity at the concentration of 100mg/mL.
authors.affiliation: School of Marine Science and Technology, Huaihai Institute of Technology, 59 Changwu Road, Lianyungang 222005, China. Electronic address: jianglongfa008@sina.com.
authors.forename: Long-Fa
authors.initials: LF
authors.lastname: Jiang
mesh_terms: D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D002480:Cellulase / Q000378:metabolism*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D027343:Rhizome / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D011134:Polysaccharides; D002480:Cellulase
keywords: Antioxidant activity; Cellulase-assisted extraction; Polysaccharides