title: Effects of laser irradiation (670-nm InGaP and 830-nm GaAlAs) on burn of second-degree in rats.
abstract: This study investigated the effects of 670-nm indium gallium phosphide (InGaP) and 830-nm gallium aluminum arsenide (GaAlAs) laser therapy on second-degree burns induced on the back of Wistar rats. Sixty-three male Wistar rats were anesthetized, and second-degree burns were made on their back. The animals were then divided randomly into three groups: control (C), animals treated with 670-nm InGaP laser (LIn), and animals treated with 830-nm GaAlAs laser (LGa). The wound areas were removed after 2, 6, 10, 14, and 18 days of treatment and submitted to structural and morphometric analysis. The following parameters were studied: total number of granulocytes and fibroblasts, number of newly formed blood vessels, and percentage of birefringent collagen fibers in the repair area. Morphometric analysis showed that different lasers 670-nm InGaP and 830-nm GaAlAs reduced the number of granulocytes and an increase of newly formed vessels in radiated lesions. The 670-nm InGaP laser therapy was more effective in increasing the number of fibroblasts. The different treatments modified the expression of VEGF and TGF-β1, when compared with lesions not irradiated. The different types of light sources showed similar effects, improved the healing of second-degree burns and can help for treating this type of injury. Despite the large number of studies with LLTI application in second-degree burns, there is still divergence about the best irradiation parameters to be used. Further studies are needed for developing a protocol effective in treating this type of injury.
authors.affiliation: School of Biomedicine, Herminio Ometto University Center, UNIARARAS, Av. Maximiliano Baruto, 500, Araras, SP , 13607-339, Brazil, gabrielachiarotto@yahoo.com.br.
authors.forename: Gabriela Bortolança
authors.initials: GB
authors.lastname: Chiarotto
authors.forename: Lia Mara Grosso
authors.initials: LM
authors.lastname: Neves
authors.forename: Marcelo Augusto Marreto
authors.initials: MA
authors.lastname: Esquisatto
authors.forename: Maria Esméria Corezola
authors.initials: ME
authors.lastname: do Amaral
authors.forename: Gláucia Maria Tech
authors.initials: GM
authors.lastname: dos Santos
authors.forename: Fernanda Aparecida Sampaio
authors.initials: FA
authors.lastname: Mendonça
mesh_terms: D000818:Animals; D001718:Birefringence; D001808:Blood Vessels / Q000473:pathology; D002056:Burns / Q000473:pathology / Q000628:therapy*; D002452:Cell Count; D005347:Fibroblasts / Q000473:pathology; D005708:Gallium / Q000737:chemistry*; D007204:Indium / Q000737:chemistry*; D054023:Lasers, Semiconductor; D028022:Low-Level Light Therapy; D008297:Male; D000073718:Pancreatitis-Associated Proteins; D010720:Phosphines / Q000737:chemistry*; D017208:Rats, Wistar; D012867:Skin / Q000473:pathology; D013194:Staining and Labeling; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D000073718:Pancreatitis-Associated Proteins; D010720:Phosphines; C000612141:REG3A protein, human; D053773:Transforming Growth Factor beta1; D042461:Vascular Endothelial Growth Factor A; D007204:Indium; D005708:Gallium; C090882:indium phosphide
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