title: Human somatic cell nuclear transfer using adult cells.
abstract: Derivation of patient-specific human pluripotent stem cells via somatic cell nuclear transfer (SCNT) has the potential for applications in a range of therapeutic contexts. However, successful SCNT with human cells has proved challenging to achieve, and thus far has only been reported with fetal or infant somatic cells. In this study, we describe the application of a recently developed methodology for the generation of human ESCs via SCNT using dermal fibroblasts from 35- and 75-year-old males. Our study therefore demonstrates the applicability of SCNT for adult human cells and supports further investigation of SCNT as a strategy for regenerative medicine.
authors.affiliation: Research Institute for Stem Cell Research, CHA Health Systems, Los Angeles, CA 90036, USA; CHA Stem Cell Institute, CHA University, Seoul 135-081, Korea. Electronic address: ychung@chahealthsystems.com.
authors.forename: Young Gie
authors.initials: YG
authors.lastname: Chung
authors.affiliation: CHA Stem Cell Institute, CHA University, Seoul 135-081, Korea.
authors.forename: Jin Hee
authors.initials: JH
authors.lastname: Eum
authors.affiliation: CHA Stem Cell Institute, CHA University, Seoul 135-081, Korea.
authors.forename: Jeoung Eun
authors.initials: JE
authors.lastname: Lee
authors.affiliation: Department of Biomedical Science, CHA University 135-081, Seoul 135-081, Korea.
authors.forename: Sung Han
authors.initials: SH
authors.lastname: Shim
authors.affiliation: CHA Fertility Center, Los Angeles, CA 90036, USA.
authors.forename: Vicken
authors.initials: V
authors.lastname: Sepilian
authors.affiliation: CHA Fertility Center, Los Angeles, CA 90036, USA.
authors.forename: Seung Wook
authors.initials: SW
authors.lastname: Hong
authors.affiliation: Research Institute for Stem Cell Research, CHA Health Systems, Los Angeles, CA 90036, USA.
authors.forename: Yumie
authors.initials: Y
authors.lastname: Lee
authors.affiliation: Reproductive Medicine Associates of New Jersey, Basking Ridge, NJ 07920, USA.
authors.forename: Nathan R
authors.initials: NR
authors.lastname: Treff
authors.affiliation: Department of Veterinary Physiology & Pharmacology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX 77843, USA.
authors.forename: Young Ho
authors.initials: YH
authors.lastname: Choi
authors.affiliation: Advanced Cell Technology, Marlborough, MA 01752, USA.
authors.forename: Erin A
authors.initials: EA
authors.lastname: Kimbrel
authors.affiliation: Stem Cell Source LLC, Houston, TX 77056, USA.
authors.forename: Ralph E
authors.initials: RE
authors.lastname: Dittman
authors.affiliation: Advanced Cell Technology, Marlborough, MA 01752, USA.
authors.forename: Robert
authors.initials: R
authors.lastname: Lanza
authors.affiliation: Research Institute for Stem Cell Research, CHA Health Systems, Los Angeles, CA 90036, USA; CHA Stem Cell Institute, CHA University, Seoul 135-081, Korea; Department of Biomedical Science, CHA University 135-081, Seoul 135-081, Korea. Electronic address: drleedr@cha.ac.kr.
authors.forename: Dong Ryul
authors.initials: DR
authors.lastname: Lee
mesh_terms: D000328:Adult; D053687:Adult Stem Cells / Q000166:cytology*; D000368:Aged; D020405:Dermis / Q000166:cytology; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D008297:Male; D053652:Nuclear Transfer Techniques; D039904:Pluripotent Stem Cells / Q000166:cytology*