title: MicroRNA repertoire for functional genome research in tilapia identified by deep sequencing.
abstract: The Nile tilapia (Oreochromis niloticus; Cichlidae) is an economically important species in aquaculture and occupies a prominent position in the aquaculture industry. MicroRNAs (miRNAs) are a class of noncoding RNAs that post-transcriptionally regulate gene expression involved in diverse biological and metabolic processes. To increase the repertoire of miRNAs characterized in tilapia, we used the Illumina/Solexa sequencing technology to sequence a small RNA library using pooled RNA sample isolated from the different developmental stages of tilapia. Bioinformatic analyses suggest that 197 conserved and 27 novel miRNAs are expressed in tilapia. Sequence alignments indicate that all tested miRNAs and miRNAs* are highly conserved across many species. In addition, we characterized the tissue expression patterns of five miRNAs using real-time quantitative PCR. We found that miR-1/206, miR-7/9, and miR-122 is abundantly expressed in muscle, brain, and liver, respectively, implying a potential role in the regulation of tissue differentiation or the maintenance of tissue identity. Overall, our results expand the number of tilapia miRNAs, and the discovery of miRNAs in tilapia genome contributes to a better understanding the role of miRNAs in regulating diverse biological processes.
authors.affiliation: Key Laboratory of Freshwater Fisheries Germplasm Resource, Ministry of Agriculture, SHOU, Shanghai, 201306, China, yanbiao1982@hotmail.com.
authors.forename: Biao
authors.initials: B
authors.lastname: Yan
authors.forename: Zhen-Hua
authors.initials: ZH
authors.lastname: Wang
authors.forename: Chang-Dong
authors.initials: CD
authors.lastname: Zhu
authors.forename: Jin-Tao
authors.initials: JT
authors.lastname: Guo
authors.forename: Jin-Liang
authors.initials: JL
authors.lastname: Zhao
mesh_terms: D000818:Animals; D017756:Aquaculture / Q000379:methods*; D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D019295:Computational Biology; D017931:DNA Primers / Q000235:genetics; D015723:Gene Library; D023281:Genomics / Q000379:methods*; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods; D008099:Liver / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics*; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D016415:Sequence Alignment; D017210:Tilapia / Q000235:genetics*
chemical_list: D017931:DNA Primers; D035683:MicroRNAs
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