title: Proteomic analysis of etiolated juvenile tetraploid Robinia pseudoacacia branches during different cutting periods.
abstract: The propagation of hard-branch cuttings of tetraploid Robinia pseudoacacia (black locust) is restricted by the low rooting rate; however, etiolated juvenile tetraploid black locust branches result in a significantly higher rooting rate of cuttings compared with non-etiolated juvenile tetraploid branches. To identify proteins that influence the juvenile tetraploid branch rooting process, two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectra (MALDI-TOF/TOF-MS) were used to analyze proteomic differences in the phloem of tetraploid R. pseudoacacia etiolated and non-etiolated juvenile branches during different cutting periods. A total of 58 protein spots differed in expression level, and 16 protein spots were only expressed in etiolated branches or non-etiolated ones. A total of 40 highly expressed protein spots were identified by mass spectrometry, 14 of which were accurately retrieved. They include nucleoglucoprotein metabolic proteins, signaling proteins, lignin synthesis proteins and phyllochlorin. These results help to reveal the mechanism of juvenile tetraploid R. pseudoacacia etiolated branch rooting and provide a valuable reference for the improvement of tetraploid R. pseudoacacia cutting techniques.
authors.affiliation: National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China. ln890110@gmail.com.
authors.forename: Nan
authors.initials: N
authors.lastname: Lu
authors.affiliation: Key Laboratory for Silviculture and Conservation, Ministry of Education, Beijing Forestry University, Beijing 100083, China. xuzhaohecn@gmail.com.
authors.forename: Zhaohe
authors.initials: Z
authors.lastname: Xu
authors.affiliation: National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China. mengbn80@gmail.com.
authors.forename: Bingnan
authors.initials: B
authors.lastname: Meng
authors.affiliation: National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China. syh831008@gmail.com.
authors.forename: Yuhan
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Henan Academy of Forestry Science, Zhengzhou 450008, Henan, China. zhjt5966@gmail.com.
authors.forename: Jiangtao
authors.initials: J
authors.lastname: Zhang
authors.affiliation: The State-Owned Luoning County Lv Cun Forest Farm, Luoning 471711, Henan, China. wangshaoming1969@gmail.com.
authors.forename: Shaoming
authors.initials: S
authors.lastname: Wang
authors.affiliation: National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China. yunli63@gmail.com.
authors.forename: Yun
authors.initials: Y
authors.lastname: Li
mesh_terms: D015180:Electrophoresis, Gel, Two-Dimensional; D064527:Etiolation; D020543:Proteome / Q000032:analysis*; D040901:Proteomics; D031301:Robinia / Q000254:growth & development / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D057891:Tetraploidy
chemical_list: D020543:Proteome
references.citation: Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao. 2004 Aug;30(4):435-40
references.pmid: 15627693
references.citation: Biochim Biophys Acta. 1983 Oct 13;741(1):103-8
references.pmid: 6615839
references.citation: Plant Mol Biol. 1999 Jan;39(1):21-34
references.pmid: 10080706
references.citation: Gene. 2009 Feb 15;431(1-2):86-94
references.pmid: 19071198
references.citation: Nature. 1982 Jun 24;297(5868):647-50
references.pmid: 7088152
references.citation: J Cell Biol. 1989 Dec;109(6 Pt 1):2617-22
references.pmid: 2556403
references.citation: Electrophoresis. 2003 Jul;24(14):2369-75
references.pmid: 12874872
references.citation: J Mol Cell Cardiol. 1996 Jul;28(7):1435-43
references.pmid: 8841931
references.citation: Plant Physiol. 2000 Dec;124(4):1718-27
references.pmid: 11115888
references.citation: Cell Mol Life Sci. 2001 Sep;58(10):1430-41
references.pmid: 11693524
references.citation: Annu Rev Cell Biol. 1986;2:499-516
references.pmid: 3030381
references.citation: J Exp Bot. 2005 Aug;56(418):2095-105
references.pmid: 15955788
references.citation: Trends Plant Sci. 2000 Dec;5(12):531-6
references.pmid: 11120475
references.citation: Plant Physiol. 2004 Jul;135(3):1526-39
references.pmid: 15247392
references.citation: Plant Physiol. 2006 Jan;140(1):349-64
references.pmid: 16377752
references.citation: Plant Physiol. 1998 Dec;118(4):1369-78
references.pmid: 9847111
references.citation: J Cell Biol. 1990 Nov;111(5 Pt 1):1793-802
references.pmid: 1699948
references.citation: Trends Biochem Sci. 1993 Jun;18(6):210-4
references.pmid: 8346556
references.citation: Plant Physiol. 2005 Feb;137(2):638-50
references.pmid: 15665246
references.citation: Plant Cell. 1993 Jan;5(1):9-23
references.pmid: 8439747
references.citation: Plant Cell. 2005 Jun;17(6):1788-800
references.pmid: 15879561
references.citation: Proc Natl Acad Sci U S A. 1997 Jul 8;94(14):7679-84
references.pmid: 9207152
references.citation: Plant Cell Physiol. 1996 Oct;37(7):957-65
references.pmid: 8979396
references.citation: Trends Plant Sci. 2004 Aug;9(8):371-7
references.pmid: 15358267
references.citation: J Plant Res. 2003 Apr;116(2):83-91
references.pmid: 12736780
references.citation: Plant Physiol. 1997 Jul;114(3):871-879
references.pmid: 12223748
references.citation: J Biol Chem. 1992 Jul 5;267(19):13585-92
references.pmid: 1339433
references.citation: Trends Plant Sci. 2000 Jul;5(7):283-90
references.pmid: 10871900
references.citation: Plant Cell. 1997 Nov;9(11):1985-98
references.pmid: 9401123
references.citation: Plant Physiol. 1998 Apr;116(4):1515-26
references.pmid: 9536070
references.citation: J Mol Biol. 1997 Jul 11;270(2):179-87
references.pmid: 9236120
references.citation: Plant J. 2001 Dec;28(5):555-67
references.pmid: 11849595
references.citation: Plant Physiol. 1995 Jul;108(3):1185-95
references.pmid: 7630941
references.citation: Plant Mol Biol. 2004 Dec;56(6):839-48
references.pmid: 15821984