title: In vitro evaluation of mucoadhesion and permeation enhancement of polymeric amphiphilic nanoparticles.
abstract: Oleoyl-carboxymethy-chitosan (OCMCS) nanoparticles based on chitosan with various molecular weights were prepared using coacervation process, which demonstrated particle size of 150-350 nm, zeta potential of 10-20 mV, and high encapsulation efficiency of fluorescein isothiocyanate dextran (FD4). OCMCS nanoparticles were found to be adsorbed onto the excised carp intestinal mucosa, the extent of adsorption increased with increasing chitosan molecular weight. In comparison to FD4 solution, OCMCS nanoparticles promoted FD4 transport through excised carp intestinal mucosa by 3.26-6.52 folds, which were observed via fluorescence microscope. The OCMCS nanoparticulate systems that interacted with the Caco-2 cells decreased the transepithelial electric resistance (TEER) and induced increasing the apparent permeability coefficient (Papp) of FD4 by 3.61-6.32 folds. Cytotoxicity studies in Caco-2 monolayers verified the safety of the delivery system. The improvement of mucoadhesive ability and permeability enable the OCMCS nanosystems suitable carriers for the intestinal absorption of protein drugs.
authors.affiliation: College of Marine Life Science, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Marine Life Science, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Di Zang
authors.affiliation: College of Marine Life Science, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Ming
authors.initials: M
authors.lastname: Kong
authors.affiliation: College of Marine Life Science, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Fang Kui
authors.initials: FK
authors.lastname: Ma
authors.affiliation: College of Marine Life Science, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Qi Feng
authors.initials: QF
authors.lastname: Dang
authors.affiliation: College of Marine Life Science, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Xiao Jie
authors.initials: XJ
authors.lastname: Cheng
authors.affiliation: The Affiliated Hospital of Medical College, Qingdao University, Qingdao 266001, PR China.
authors.forename: Qiu Xia
authors.initials: QX
authors.lastname: Ji
authors.affiliation: College of Marine Life Science, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Xi Guang
authors.initials: XG
authors.lastname: Chen
mesh_terms: D000268:Adhesiveness; D000818:Animals; D018938:Caco-2 Cells; D002347:Carps; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology*; D004337:Drug Carriers / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000378:metabolism; D007421:Intestine, Small / Q000378:metabolism; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry; D010539:Permeability
chemical_list: D004337:Drug Carriers; D048271:Chitosan