title: Whole venom of Loxosceles similis activates caspases-3, -6, -7, and -9 in human primary skin fibroblasts.
abstract: Spiders of the Loxosceles genus represent a risk to human health due to the systemic and necrotic effects of their bites. The main symptoms of these bites vary from dermonecrosis, observed in the majority of cases, to occasional systemic hemolysis and coagulopathy. Although the systemic effects are well characterized, the mechanisms of cell death triggered by the venom of these spiders are poorly characterized. In this study, we investigated the cell death mechanisms induced by the whole venom of the spider Loxosceles similis in human skin fibroblasts. Our results show that the venom initiates an apoptotic process and a caspase cascade involving the initiator caspase-9 and the effector caspases-3, -6, and -7.
authors.affiliation: Departmento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Minas Gerais, Brazil.
authors.forename: Arthur Estanislau
authors.initials: AE
authors.lastname: Dantas
authors.affiliation: Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Minas Gerais, Brazil; Programa de Pós-Graduação em Fisiologia e Farmacologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Minas Gerais, Brazil.
authors.forename: Carolina Campolina Rebello
authors.initials: CC
authors.lastname: Horta
authors.affiliation: Departmento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Minas Gerais, Brazil; Programa de Pós-graduação em Biologia Celular, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Minas Gerais, Brazil.
authors.forename: Thais M M
authors.initials: TM
authors.lastname: Martins
authors.affiliation: Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Minas Gerais, Brazil.
authors.forename: Anderson Oliveira
authors.initials: AO
authors.lastname: do Carmo
authors.affiliation: Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Minas Gerais, Brazil.
authors.forename: Bárbara Bruna Ribeiro de Oliveira
authors.initials: BB
authors.lastname: Mendes
authors.affiliation: Departmento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Minas Gerais, Brazil.
authors.forename: Alfredo M
authors.initials: AM
authors.lastname: Goes
authors.affiliation: Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Minas Gerais, Brazil.
authors.forename: Evanguedes
authors.initials: E
authors.lastname: Kalapothakis
authors.affiliation: Departmento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Minas Gerais, Brazil. Electronic address: dawidson@icb.ufmg.br.
authors.forename: Dawidson A
authors.initials: DA
authors.lastname: Gomes
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D020169:Caspases / Q000378:metabolism*; D002470:Cell Survival / Q000187:drug effects; D004789:Enzyme Activation / Q000187:drug effects*; D005347:Fibroblasts / Q000187:drug effects* / Q000201:enzymology*; D006801:Humans; D061251:Primary Cell Culture; D012867:Skin / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D013111:Spider Venoms / Q000494:pharmacology*; D013112:Spiders / Q000502:physiology
chemical_list: D013111:Spider Venoms; D020169:Caspases
keywords: Apoptosis; Caspases; Cell death; Loxosceles similis; Necrosis; Venom
references.citation: Toxicon. 2010 Sep 15;56(4):535-43
references.pmid: 20573594
references.citation: Toxicon. 2013 Aug;70:90-7
references.pmid: 23648420
references.citation: PLoS Negl Trop Dis. 2013 May 02;7(5):e2206
references.pmid: 23658852
references.citation: J Invest Dermatol. 2006 Jan;126(1):61-8
references.pmid: 16417218
references.citation: J Immunol Methods. 1983 Dec 16;65(1-2):55-63
references.pmid: 6606682
references.citation: Cell Biochem Funct. 2011 Aug;29(6):468-80
references.pmid: 21773978
references.citation: Toxicol Appl Pharmacol. 2006 Feb 15;211(1):64-77
references.pmid: 16005484
references.citation: Toxicon. 2013 Jun 1;67:17-30
references.pmid: 23462381
references.citation: Toxicon. 2005 Mar 15;45(4):489-99
references.pmid: 15733571
references.citation: J Immunol. 2012 Feb 15;188(4):1992-2000
references.pmid: 22246630
references.citation: Toxicon. 2004 Dec 1;44(7):693-709
references.pmid: 15500846
references.citation: Toxicon. 2010 Dec;56(8):1426-35
references.pmid: 20826175
references.citation: Toxicon. 2012 Sep 1;60(3):396-405
references.pmid: 22538195
references.citation: Toxicon. 2012 Jul;60(1):21-30
references.pmid: 22465492
references.citation: J Vis Exp. 2010 Oct 05;(44):
references.pmid: 20972406
references.citation: Cell Death Differ. 2012 Jan;19(1):107-20
references.pmid: 21760595
references.citation: Histochem J. 2000 Jul;32(7):397-408
references.pmid: 10987503
references.citation: J Invest Dermatol. 2007 Jun;127(6):1410-8
references.pmid: 17218940
references.citation: Toxicon. 2002 Jun;40(6):815-22
references.pmid: 12175619
references.citation: J Cell Biochem. 2011 Sep;112(9):2529-40
references.pmid: 21590705
references.citation: Braz J Med Biol Res. 2001 Jul;34(7):843-50
references.pmid: 11449301
references.citation: J Invest Dermatol. 2013 Jun;133(6):1682-5
references.pmid: 23353984
references.citation: J Cell Biochem. 2009 Jul 1;107(4):655-66
references.pmid: 19455508
references.citation: Cell Death Differ. 2008 Sep;15(9):1339-49
references.pmid: 18566602
references.citation: Nat Rev Mol Cell Biol. 2008 Mar;9(3):231-41
references.pmid: 18073771
references.citation: Cell. 1996 Oct 18;87(2):171
references.pmid: 8861900