title: Modeling nanoparticle-alveolar epithelial cell interactions under breathing conditions using captive bubble surfactometry.
abstract: Many advances have been made in recent years in cell culture models of the epithelial barrier of the lung from simple monolayers to complex 3-D systems employing different cell types. However, the vast majority of these models still present a static air-liquid interface which is unrealistic given the dynamic nature of breathing. We present here a method where epithelial lung cells are integrated into a system, the captive bubble surfactometer, which allows the cyclical compression and expansion of the surfactant film at the air-liquid interface, thus modeling the dynamics of breathing. We found that cellular uptake of deposited gold nanoparticles was significantly increased under the dynamic (breathing) conditions of compression and expansion as compared to static conditions. The method could be very useful for studying nanoparticle-alveolar lung cell interactions under breathing conditions for applications in nanomedicine and toxicology.
authors.affiliation: Adolphe Merkle Institute, University of Fribourg , Fribourg, Switzerland.
authors.forename: David
authors.initials: D
authors.lastname: Schürch
authors.forename: Dimitri
authors.initials: D
authors.lastname: Vanhecke
authors.forename: Martin J D
authors.initials: MJ
authors.lastname: Clift
authors.forename: David
authors.initials: D
authors.lastname: Raemy
authors.forename: Dorleta Jimenez
authors.initials: DJ
authors.lastname: de Aberasturi
authors.forename: Wolfgang J
authors.initials: WJ
authors.lastname: Parak
authors.forename: Peter
authors.initials: P
authors.lastname: Gehr
authors.forename: Alke
authors.initials: A
authors.lastname: Petri-Fink
authors.forename: Barbara
authors.initials: B
authors.lastname: Rothen-Rutishauser
mesh_terms: D004847:Epithelial Cells / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D011650:Pulmonary Alveoli / Q000166:cytology*; D013501:Surface-Active Agents / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D013501:Surface-Active Agents