title: A tobacco homolog of DCN1 is involved in pollen development and embryogenesis.
abstract: We show that DCN1 binds ubiquitin and RUB/NEDD8, associates with cullin, and is functionally conserved. DCN1 activity is required for pollen development transitions and embryogenesis, and for pollen tube growth. Plant proteomes show remarkable plasticity in reaction to environmental challenges and during developmental transitions. Some of this adaptability comes from ubiquitin-mediated protein degradation regulated by cullin-RING E3 ubiquitin ligases (CRLs). CRLs are activated through modification of the cullin subunit with the ubiquitin-like protein RUB/NEDD8 by an E3 ligase called defective in cullin neddylation 1 (DCN1). Here we show that tobacco DCN1 binds ubiquitin and RUB/NEDD8 and associates with cullin. When knocked down by RNAi, tobacco pollen formation was affected and zygotic embryogenesis was blocked around the globular stage. Additionally, we found that RNAi of DCN1 inhibited the stress-triggered reprogramming of cultured microspores from their intrinsic gametophytic mode of development to an embryogenic state. This stress-induced developmental switch is a known feature in many important crops and leads ultimately to the formation of haploid embryos and plants. Compensating the RNAi effect by re-transformation with a promoter-silencing construct restored pollen development and zygotic embryogenesis, as well as the ability for stress-induced formation of embryogenic microspores. Overexpression of DCN1 accelerated pollen tube growth and increased the potential for microspore reprogramming. These results demonstrate that the biochemical function of DCN1 is conserved in plants and that its activity is involved in transitions during pollen development and embryogenesis, and for pollen tube growth.
authors.affiliation: Max F. Perutz Laboratories, Vienna University, Dr. Bohrgasse 9, 1030, Vienna, Austria.
authors.forename: Julia
authors.initials: J
authors.lastname: Hosp
authors.forename: Alexandra
authors.initials: A
authors.lastname: Ribarits
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Retzer
authors.forename: Yongfeng
authors.initials: Y
authors.lastname: Jin
authors.forename: Alisher
authors.initials: A
authors.lastname: Tashpulatov
authors.forename: Tatiana
authors.initials: T
authors.lastname: Resch
authors.forename: Christina
authors.initials: C
authors.lastname: Friedmann
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Ankele
authors.forename: Viktor
authors.initials: V
authors.lastname: Voronin
authors.forename: Klaus
authors.initials: K
authors.lastname: Palme
authors.forename: Erwin
authors.initials: E
authors.lastname: Heberle-Bors
authors.forename: Alisher
authors.initials: A
authors.lastname: Touraev
mesh_terms: D000595:Amino Acid Sequence; D029742:Caenorhabditis elegans Proteins / Q000235:genetics; D044842:Cullin Proteins / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D000075687:NEDD8 Protein; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D011058:Pollen / Q000254:growth & development*; D034622:RNA Interference; D012639:Seeds / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D014026:Nicotiana / Q000235:genetics* / Q000254:growth & development; D025801:Ubiquitin / Q000378:metabolism; D014452:Ubiquitins / Q000235:genetics / Q000378:metabolism
chemical_list: D029742:Caenorhabditis elegans Proteins; C100318:Cullin 1; D044842:Cullin Proteins; D000075687:NEDD8 Protein; C082860:NEDD8 protein, human; D010940:Plant Proteins; D025801:Ubiquitin; D014452:Ubiquitins; C502136:dcn-1 protein, C elegans
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