title: Effect of Cbfa1 on osteogenic differentiation of mesenchymal stem cells under hypoxia condition.
abstract: OBJECTIVE
  To observe the effect of Cbfa1 on biological characteristics of marrow mesenchymal stem cells under hypoxia.
  
  
  METHODS
  The second passage of the MSCs were transfected with Cbfa1 and then cultured in 20% O2 and 3% O2 condition individually. The biological features of the cultured MSCs were assessed by the Real-time PCR.
  
  
  RESULTS
  After transfected with Cbfa1, the morphology of MSCs was no significant difference between two oxygen concentrations; The RT-PCR examination revealed that the expression of Cbfa1, BMP, OPN and VEGF in MSCs was higher than those before Cbfa1 transfection (P<0.05), especial the expression of Cbfa1 (P<0.05).
  
  
  CONCLUSIONS
  After transfected with Cbfa1, cell morphology or growth cycle of MSCs was not significantly affected, but its osteogenic differentiation potential enhanced, particularly its osteogenic differentiation potential under hypoxia enhanced.
authors.affiliation: Department of Orthopedics, Renmin Hospital of Wuhan University Wuhan 430060, China.
authors.forename: Zhang-Hua
authors.initials: ZH
authors.lastname: Li
authors.affiliation: Hubei University of Chinese Medicine Wuhan 430065, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Liao
authors.affiliation: Laboratory of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Academy of Military Medical Sciences Beijing 100850, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Department of Orthopedics, Renmin Hospital of Wuhan University Wuhan 430060, China.
authors.forename: Tang
authors.initials: T
authors.lastname: Huan
authors.affiliation: Department of Orthopedics, Renmin Hospital of Wuhan University Wuhan 430060, China.
authors.forename: Pan
authors.initials: P
authors.lastname: Feng
authors.affiliation: Laboratory of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Academy of Military Medical Sciences Beijing 100850, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Wei
authors.affiliation: Wuhan Sports University Wuhan 430079, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yi
authors.affiliation: Laboratory of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Academy of Military Medical Sciences Beijing 100850, China.
authors.forename: Ning-Sheng
authors.initials: NS
authors.lastname: Shao
keywords: Core binding factor alpha l; Mesenchymal stem cell; hypoxia
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