title: Bacteria and genes involved in arsenic speciation in sediment impacted by long-term gold mining.
abstract: The bacterial community and genes involved in geobiocycling of arsenic (As) from sediment impacted by long-term gold mining were characterized through culture-based analysis of As-transforming bacteria and metagenomic studies of the arsC, arrA, and aioA genes. Sediment was collected from the historically gold mining impacted Mina stream, located in one of the world's largest mining regions known as the "Iron Quadrangle". A total of 123 As-resistant bacteria were recovered from the enrichment cultures, which were phenotypically and genotypically characterized for As-transformation. A diverse As-resistant bacteria community was found through phylogenetic analyses of the 16S rRNA gene. Bacterial isolates were affiliated with Proteobacteria, Firmicutes, and Actinobacteria and were represented by 20 genera. Most were AsV-reducing (72%), whereas AsIII-oxidizing accounted for 20%. Bacteria harboring the arsC gene predominated (85%), followed by aioA (20%) and arrA (7%). Additionally, we identified two novel As-transforming genera, Thermomonas and Pannonibacter. Metagenomic analysis of arsC, aioA, and arrA sequences confirmed the presence of these genes, with arrA sequences being more closely related to uncultured organisms. Evolutionary analyses revealed high genetic similarity between some arsC and aioA sequences obtained from isolates and clone libraries, suggesting that those isolates may represent environmentally important bacteria acting in As speciation. In addition, our findings show that the diversity of arrA genes is wider than earlier described, once none arrA-OTUs were affiliated with known reference strains. Therefore, the molecular diversity of arrA genes is far from being fully explored deserving further attention.
authors.affiliation: Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais; Belo Horizonte, Brazil.
authors.forename: Patrícia S
authors.initials: PS
authors.lastname: Costa
authors.affiliation: Grupo de Genômica e Biologia Computacional, Centro de Pesquisas René Rachou (CPqRR), Fundação Oswaldo Cruz (FIOCRUZ), Belo Horizonte, Brazil.
authors.forename: Larissa L S
authors.initials: LL
authors.lastname: Scholte
authors.affiliation: Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais; Belo Horizonte, Brazil.
authors.forename: Mariana P
authors.initials: MP
authors.lastname: Reis
authors.affiliation: Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais; Belo Horizonte, Brazil.
authors.forename: Anderson V
authors.initials: AV
authors.lastname: Chaves
authors.affiliation: Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais; Belo Horizonte, Brazil.
authors.forename: Pollyanna L
authors.initials: PL
authors.lastname: Oliveira
authors.affiliation: Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais; Belo Horizonte, Brazil.
authors.forename: Luiza B
authors.initials: LB
authors.lastname: Itabayana
authors.affiliation: Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais; Belo Horizonte, Brazil.
authors.forename: Maria Luiza S
authors.initials: ML
authors.lastname: Suhadolnik
authors.affiliation: Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais; Belo Horizonte, Brazil.
authors.forename: Francisco A R
authors.initials: FA
authors.lastname: Barbosa
authors.affiliation: Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais; Belo Horizonte, Brazil.
authors.forename: Edmar
authors.initials: E
authors.lastname: Chartone-Souza
authors.affiliation: Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais; Belo Horizonte, Brazil.
authors.forename: Andréa M A
authors.initials: AM
authors.lastname: Nascimento
mesh_terms: D001151:Arsenic / Q000378:metabolism*; D001419:Bacteria / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D005075:Biological Evolution; D001711:Biotransformation; D005798:Genes, Bacterial; D005838:Genotype; D019015:Geologic Sediments / Q000737:chemistry*; D006046:Gold; D055432:Metabolomics; D008906:Mining; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010641:Phenotype; D010802:Phylogeny; D013997:Time Factors; D014867:Water / Q000737:chemistry
chemical_list: D014867:Water; D006046:Gold; D001151:Arsenic
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