title: Global transcriptional analysis reveals surface remodeling of Anaplasma marginale in the tick vector.
abstract: BACKGROUND
  Pathogens dependent upon vectors for transmission to new hosts undergo environment specific changes in gene transcription dependent on whether they are replicating in the vector or the mammalian host. Differential gene transcription, especially of potential vaccine candidates, is of interest in Anaplasma marginale, the tick-borne causative agent of bovine anaplasmosis.
  
  
  METHODS
  RNA-seq technology allowed a comprehensive analysis of the transcriptional status of A. marginale genes in two conditions: bovine host blood and tick derived cell culture, a model for the tick vector. Quantitative PCR was used to assess transcription of a set of genes in A. marginale infected tick midguts and salivary glands at two time points during the transmission cycle.
  
  
  RESULTS
  Genes belonging to fourteen pathways or component groups were found to be differentially transcribed in A. marginale in the bovine host versus the tick vector. One of the most significantly altered groups was composed of surface proteins. Of the 56 genes included in the surface protein group, eight were up regulated and 26 were down regulated. The down regulated surface protein encoding genes include several that are well studied due to their immunogenicity and function. Quantitative PCR of a set of genes demonstrated that transcription in tick cell culture most closely approximates transcription in salivary glands of recently infected ticks.
  
  
  CONCLUSIONS
  The ISE6 tick cell culture line is an acceptable model for early infection in tick salivary glands, and reveals disproportionate down regulation of surface protein genes in the tick. Transcriptional profiling in other cell lines may help us simulate additional microenvironments. Understanding vector-specific alteration of gene transcription, especially of surface protein encoding genes, may aid in the development of vaccines or transmission blocking therapies.
authors.forename: G Kenitra
authors.initials: GK
authors.lastname: Hammac
authors.forename: Sebastián Aguilar
authors.initials: SA
authors.lastname: Pierlé
authors.forename: Xiaoya
authors.initials: X
authors.lastname: Cheng
authors.forename: Glen A
authors.initials: GA
authors.lastname: Scoles
authors.affiliation: Program in Genomics, Department of Veterinary Microbiology and Pathology, Paul G, Allen School for Global Animal Health, Washington State University, Pullman, WA 99164-7040, USA. kbrayton@vetmed.wsu.edu.
authors.forename: Kelly A
authors.initials: KA
authors.lastname: Brayton
mesh_terms: D042322:Anaplasma marginale / Q000502:physiology*; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D003870:Dermacentor / Q000382:microbiology*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D008565:Membrane Proteins
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