title: Duck enteritis virus glycoprotein D and B DNA vaccines induce immune responses and immunoprotection in Pekin ducks.
abstract: DNA vaccine is a promising strategy for protection against virus infection. However, little is known on the efficacy of vaccination with two plasmids for expressing the glycoprotein D (gD) and glycoprotein B (gB) of duck enteritis virus (DEV) in inducing immune response and immunoprotection against virulent virus infection in Pekin ducks. In this study, two eukaryotic expressing plasmids of pcDNA3.1-gB and pcDNA3.1-gD were constructed. Following transfection, the gB and gD expressions in DF1 cells were detected. Groups of ducks were vaccinated with pcDNA3.1-gB and/or pcDNA3.1-gD, and boosted with the same vaccine on day 14 post primary vaccination. We found that intramuscular vaccinations with pcDNA3.1-gB and/or pcDNA3.1-gD, but not control plasmid, stimulated a high frequency of CD4+ and CD8+ T cells in Pekin ducks, particularly with both plasmids. Similarly, vaccination with these plasmids, particularly with both plasmids, promoted higher levels of neutralization antibodies against DEV in Pekin ducks. More importantly, vaccination with both plasmids significantly reduced the virulent DEV-induced mortality in Pekin ducks. Our data indicated that vaccination with plasmids for expressing both gB and gD induced potent cellular and humoral immunity against DEV in Pekin ducks. Therefore, this vaccination strategy may be used for the prevention of DEV infection in Pekin ducks.
authors.affiliation: Group of Avian Respiratory infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, China; Northeast Agricultural University, Harbin, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Northeast Agricultural University, Harbin, China.
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Northeast Agricultural University, Harbin, China.
authors.forename: Lihong
authors.initials: L
authors.lastname: Cui
authors.affiliation: Northeast Agricultural University, Harbin, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Ma
authors.affiliation: Northeast Agricultural University, Harbin, China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Mu
authors.affiliation: Northeast Agricultural University, Harbin, China.
authors.forename: Yanwei
authors.initials: Y
authors.lastname: Li
authors.affiliation: Northeast Agricultural University, Harbin, China.
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Northeast Agricultural University, Harbin, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Li
authors.affiliation: Northeast Agricultural University, Harbin, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wei
authors.affiliation: Northeast Agricultural University, Harbin, China.
authors.forename: Mingchun
authors.initials: M
authors.lastname: Gao
authors.affiliation: Northeast Agricultural University, Harbin, China.
authors.forename: Junwei
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D004372:Ducks; D007111:Immunity, Cellular; D056724:Immunity, Humoral; D007074:Immunoglobulin G / Q000276:immunology; D025922:Mardivirus / Q000235:genetics* / Q000276:immunology*; D011201:Poultry Diseases / Q000276:immunology* / Q000517:prevention & control*; D014611:Vaccination; D019444:Vaccines, DNA / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G; D019444:Vaccines, DNA; D014759:Viral Envelope Proteins
references.citation: Proc Natl Acad Sci U S A. 1987 Aug;84(15):5454-8
references.pmid: 3037552
references.citation: J Virol. 1984 Oct;52(1):293-7
references.pmid: 6207311
references.citation: Virol J. 2011 May 10;8:214
references.pmid: 21569289
references.citation: Avian Dis. 1993 Oct-Dec;37(4):1142-6
references.pmid: 8141746
references.citation: Immunology. 1993 Jan;78(1):7-13
references.pmid: 8382189
references.citation: JAMA. 1999 Jul 28;282(4):331-40
references.pmid: 10432030
references.citation: J Virol. 1992 May;66(5):2631-8
references.pmid: 1313890
references.citation: J Virol. 1988 Nov;62(11):4239-48
references.pmid: 2845135
references.citation: DNA Cell Biol. 2003 Jul;22(7):475-8
references.pmid: 12932306
references.citation: J Gen Virol. 1995 Jul;76 ( Pt 7):1623-35
references.pmid: 9049369
references.citation: Virology. 1997 Feb 3;228(1):39-54
references.pmid: 9024808
references.citation: J Infect Dis. 2003 Feb 15;187(4):542-9
references.pmid: 12599070
references.citation: Virology. 1995 May 10;209(1):230-5
references.pmid: 7747474
references.citation: J Virol. 1987 Nov;61(11):3356-64
references.pmid: 2444713
references.citation: Avian Dis. 1979 Oct-Dec;23(4):940-9
references.pmid: 232659
references.citation: Intervirology. 2001;44(1):1-7
references.pmid: 11223713
references.citation: Vaccine. 1993;11(1):25-35
references.pmid: 8381248
references.citation: J Virol. 1984 Mar;49(3):1014-7
references.pmid: 6321752
references.citation: Infect Immun. 1984 Feb;43(2):761-4
references.pmid: 6319291
references.citation: J Virol. 1990 May;64(5):2399-406
references.pmid: 2157895
references.citation: J Virol. 1990 Jun;64(6):2491-7
references.pmid: 2159526
references.citation: Virology. 1992 May;188(1):256-64
references.pmid: 1314454
references.citation: Virology. 1987 Jul;159(1):57-66
references.pmid: 3037783
references.citation: J Immunol. 1998 Jun 15;160(12):6093-100
references.pmid: 9637526
references.citation: Proc Natl Acad Sci U S A. 1987 Aug;84(16):5908-12
references.pmid: 3303033
references.citation: EMBO J. 1986 Nov;5(11):3057-63
references.pmid: 3024973
references.citation: N Engl J Med. 2002 Nov 21;347(21):1652-61
references.pmid: 12444179
references.citation: Vaccine. 1990 Aug;8(4):358-68
references.pmid: 1697722
references.citation: Vaccine. 1993;11(4):407-14
references.pmid: 8385842
references.citation: J Immunol Methods. 2000 Nov 1;245(1-2):109-18
references.pmid: 11042288
references.citation: J Virol. 1990 Jan;64(1):431-6
references.pmid: 2152829
references.citation: Vaccine. 1991 Mar;9(3):147-53
references.pmid: 1645898
references.citation: Microbiol Immunol. 1984;28(8):923-33
references.pmid: 6094991
references.citation: Clin Diagn Lab Immunol. 2005 Dec;12(12):1364-9
references.pmid: 16339058