title: Early dengue diagnosis by nonstructural protein 1 antigen detection: rapid immunochromotography versus two the enzyme-linked immunosorbent assay kits.
abstract: Dengue is known for its serious life-threatening complications. New rapid kits available recently in India target circulating non-structural protein (NS1) antigen from day one onwards. The sensitivity and specificity of a newly introduced rapid combo kit against two conventional ELISA kits is assessed. The performance of this kit is quite satisfactory since excellent agreement of 94.26% was observed with particular reference to NS1 antigen detection among all three kits namely Rapid SD Bioline dengue Duo (SD Korea), InBios DENV Detect NS1 ELISA, USA and dengue Early ELISA, Panbio, Australia. The false positivity of the rapid kit is very low since its specificity as for as NS1 antigen detection is concerned is 98.33%. The use of combination kit helps to detect additional cases of dengue, which are negative for NS1 antigen but positive for IgM and/or IgG antibodies, thus facilitating early diagnosis in remote areas and small laboratorie.
authors.affiliation: Department of Microbiology, Mahatma Gandhi Medical College and Research Institute, Pillaiyarkuppam, Puducherry, India.
authors.forename: Selvaraj
authors.initials: S
authors.lastname: Stephen
authors.forename: Marie Victor P
authors.initials: MV
authors.lastname: Charles
authors.forename: Velmurugan
authors.initials: V
authors.lastname: Anitharaj
authors.forename: Civamany
authors.initials: C
authors.lastname: Deepa
authors.forename: Sivaraman
authors.initials: S
authors.lastname: Umadevi
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000956:Antigens, Viral / Q000097:blood*; D002648:Child; D002846:Chromatography, Affinity / Q000379:methods*; D003715:Dengue / Q000175:diagnosis*; D003955:Diagnostic Tests, Routine / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D012680:Sensitivity and Specificity; D017361:Viral Nonstructural Proteins / Q000097:blood*; D055815:Young Adult
chemical_list: D000956:Antigens, Viral; D017361:Viral Nonstructural Proteins