title: Rapid HPLC-MS method for the simultaneous determination of tea catechins and folates.
abstract: An effective and rapid HPLC-MS method for the simultaneous separation of the eight most abundant tea catechins, gallic acid, and caffeine was developed. These compounds were rapidly separated within 9 min by a linear gradient elution using a Zorbax SB-C18 packed with sub 2 μm particles. This methodology did not require preparative and semipreparative HPLC steps. In fact, diluted tea samples can be easily analyzed using HPLC-MS as described in this study. The use of mass spectrometry detection for quantification of catechins ensured a higher specificity of the method. The percent relative standard deviation was generally lower than 4 and 7% for most of the compounds tested in tea drinks and tea extracts, respectively. Furthermore, the method provided excellent resolution for folate determination alone or in combination with catechins. To date, no HPLC method able to discriminate catechins and folates in a quick analysis has been reported in the literature.
authors.affiliation: Institute of Nutrition and Functional Foods (INAF) and ‡Department of Food Sciences and Nutrition, Laval University , Quebec, QC, Canada G1V 0A6.
authors.forename: Monica
authors.initials: M
authors.lastname: Araya-Farias
authors.forename: Alain
authors.initials: A
authors.lastname: Gaudreau
authors.forename: Elodie
authors.initials: E
authors.lastname: Rozoy
authors.forename: Laurent
authors.initials: L
authors.lastname: Bazinet
mesh_terms: D028241:Camellia sinensis / Q000737:chemistry*; D002392:Catechin / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005492:Folic Acid / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods*; D010936:Plant Extracts / Q000032:analysis*
chemical_list: D010936:Plant Extracts; D002392:Catechin; D005492:Folic Acid