title: Reducing NO and N₂O emission during aerobic denitrification by newly isolated Pseudomonas stutzeri PCN-1.
abstract: As two obligatory intermediates of denitrification, both NO and N2O had harmful environmental and biological impacts. An aerobic denitrifying bacterial strain PCN-1 was newly isolated and identified as Pseudomonas stutzeri, which was capable of high efficient nitrogen removal under aerobic condition with maximal NO and N2O accumulation as low as 0.003% and 0.33% of removed NO3(-)-N, respectively. Further experiment taking nitrite as denitrifying substrate indicated similar low NO and N2O emission of 0.006% and 0.29% of reduced NO2(-)-N, respectively. Reverse transcription-polymerase chain reaction (RT-PCR) analysis revealed that the coordinate expression of denitrification gene nirS (for cytochrome cd1 nitrite reductase), cnorB (for NO reductase) and nosZ (for N2O reductase) was the fundamental reason of low NO and N2O accumulation. Activated sludge system bioaugmented by strain PCN-1 demonstrated a significant reduction of NO and N2O emission from wastewater during aerobic denitrification, implied great potential of PCN-1 in practical applications.
authors.affiliation: Department of Environmental Engineering, Peking University, Key Laboratory of Water and Sediment Sciences, Ministry of Education, Beijing 100871, China.
authors.forename: Maosheng
authors.initials: M
authors.lastname: Zheng
authors.affiliation: Department of Environmental Engineering, Peking University, Key Laboratory of Water and Sediment Sciences, Ministry of Education, Beijing 100871, China.
authors.forename: Da
authors.initials: D
authors.lastname: He
authors.affiliation: Department of Environmental Engineering, Peking University, Key Laboratory of Water and Sediment Sciences, Ministry of Education, Beijing 100871, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Ma
authors.affiliation: Department of Environmental Engineering, Peking University, Key Laboratory of Water and Sediment Sciences, Ministry of Education, Beijing 100871, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Department of Environmental Engineering, Peking University, Key Laboratory of Water and Sediment Sciences, Ministry of Education, Beijing 100871, China.
authors.forename: Sitong
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Environmental Engineering, Peking University, Key Laboratory of Water and Sediment Sciences, Ministry of Education, Beijing 100871, China.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Ahmad
authors.affiliation: Department of Environmental Engineering, Peking University, Key Laboratory of Water and Sediment Sciences, Ministry of Education, Beijing 100871, China.
authors.forename: Mengyao
authors.initials: M
authors.lastname: Gui
authors.affiliation: Department of Environmental Engineering, Peking University, Key Laboratory of Water and Sediment Sciences, Ministry of Education, Beijing 100871, China. Electronic address: nijinren@iee.pku.edu.cn.
authors.forename: Jinren
authors.initials: J
authors.lastname: Ni
mesh_terms: D000332:Aerobiosis / Q000187:drug effects; D000393:Air Pollutants / Q000032:analysis*; D001483:Base Sequence; D058440:Denitrification / Q000187:drug effects; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D009566:Nitrates / Q000378:metabolism; D009569:Nitric Oxide / Q000032:analysis*; D009584:Nitrogen / Q000378:metabolism; D009609:Nitrous Oxide / Q000032:analysis*; D010100:Oxygen / Q000494:pharmacology; D010802:Phylogeny; D044223:Pseudomonas stutzeri / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D000393:Air Pollutants; D009566:Nitrates; D012336:RNA, Ribosomal, 16S; D009569:Nitric Oxide; D009609:Nitrous Oxide; D009584:Nitrogen; D010100:Oxygen
keywords: Aerobic denitrification; NO and N(2)O emission; Nitrogen removal; Pseudomonas stutzeri