title: A simple plate-assay for screening extracellular naringinase produced by streptomycetes.
abstract: A simple plate-assay was developed with the purpose of detecting extracellular naringinase in streptomycetes. The naringin agar medium (NAM) was prepared by mixing carbon utilization medium (ISP9) and naringin. The clearing zones around colonies are correlated with the enzymatic activity. The assay validation was performed through the use of commercial naringinase (Penicillium decumbens).
authors.affiliation: Universidad Autónoma del Carmen, Ciudad del Carmen, Campeche México.
authors.forename: Leonela
authors.initials: L
authors.lastname: Caraveo
authors.affiliation: Universidad Autónoma del Carmen, Ciudad del Carmen, Campeche México.
authors.forename: Héctor
authors.initials: H
authors.lastname: Medina
authors.affiliation: Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C. (CIATEJ, A.C.) Unidad Sureste, Yucatán, México.
authors.forename: Ingrid
authors.initials: I
authors.lastname: Rodríguez-Buenfil
authors.affiliation: Universidad Autónoma del Carmen, Ciudad del Carmen, Campeche México.
authors.forename: Carlos
authors.initials: C
authors.lastname: Montalvo-Romero
authors.affiliation: Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C. (CIATEJ, A.C.) Unidad Sureste, Yucatán, México. Electronic address: zevangelista@ciatej.mx.
authors.forename: Zahaed
authors.initials: Z
authors.lastname: Evangelista-Martínez
mesh_terms: D000192:Actinomycetales / Q000378:metabolism*; D000362:Agar; D001431:Bacteriological Techniques / Q000379:methods*; D003470:Culture Media / Q000737:chemistry*; D009097:Multienzyme Complexes / Q000032:analysis*; D001617:beta-Glucosidase / Q000032:analysis*
chemical_list: D003470:Culture Media; D009097:Multienzyme Complexes; D000362:Agar; C039827:naringinase; D001617:beta-Glucosidase
keywords: Naringin agar medium; Naringinase; Streptomycetes