title: Comparison of stem/progenitor cell number and transcriptomic profile in the mammary tissue of dairy and beef breed heifers.
abstract: Bovine mammary stem cells (MaSC) are a source of ductal and lobulo-alveolar tissue during the development of the mammary gland and its remodeling in repeating lactation cycles. We hypothesize that the number of MaSC, their molecular properties, and interactions with their niche may be essential in order to determine the mammogenic potential in heifers. To verify this hypothesis, we compared the number of MaSC and the transcriptomic profile in the mammary tissue of 20-month-old, non-pregnant dairy (Holstein-Friesian, HF) and beef (Limousin, LM) heifers. For the identification and quantification of putative stem/progenitor cells in mammary tissue sections, scanning cytometry was used with a combination of MaSC molecular markers: stem cell antigen-1 (Sca-1) and fibronectin type III domain containing 3B (FNDC3B) protein. Cytometric analysis revealed a significantly higher number of Sca-1(pos)FNDC3B(pos) cells in HF (2.94 ± 0.35%) than in LM (1.72 ± 0.20%) heifers. In HF heifers, a higher expression of intramammary hormones, growth factors, cytokines, chemokines, and transcription regulators was observed. The model of mammary microenvironment favorable for MaSC was associated with the regulation of genes involved in MaSC maintenance, self-renewal, proliferation, migration, differentiation, mammary tissue remodeling, angiogenesis, regulation of adipocyte differentiation, lipid metabolism, and steroid and insulin signaling. In conclusion, the mammogenic potential in postpubertal dairy heifers is facilitated by a higher number of MaSC and up-regulation of mammary auto- and paracrine factors representing the MaSC niche.
authors.affiliation: Department of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences-SGGW, Nowoursynowska 159, 02-776, Warsaw, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Osińska
authors.forename: Zofia
authors.initials: Z
authors.lastname: Wicik
authors.forename: Michał M
authors.initials: MM
authors.lastname: Godlewski
authors.forename: Karol
authors.initials: K
authors.lastname: Pawłowski
authors.forename: Alicja
authors.initials: A
authors.lastname: Majewska
authors.forename: Joanna
authors.initials: J
authors.lastname: Mucha
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Gajewska
authors.forename: Tomasz
authors.initials: T
authors.lastname: Motyl
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism*; D002417:Cattle; D002452:Cell Count; D003612:Dairying; D005260:Female; D020869:Gene Expression Profiling; D007774:Lactation / Q000378:metabolism*; D008321:Mammary Glands, Animal / Q000166:cytology* / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D011247:Pregnancy; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism*
chemical_list: D015415:Biomarkers
references.citation: J Dairy Sci. 2009 Sep;92(9):4276-89
references.pmid: 19700688
references.citation: Domest Anim Endocrinol. 2000 Aug;19(2):93-104
references.pmid: 11025189
references.citation: Blood. 2013 May 16;121(20):4082-9
references.pmid: 23520338
references.citation: PLoS One. 2012;7(1):e30113
references.pmid: 22253899
references.citation: J Dairy Sci. 1988 Oct;71(10):2855-74
references.pmid: 3060494
references.citation: Blood. 2009 Feb 12;113(7):1432-43
references.pmid: 18854576
references.citation: Mol Cell Biol. 2004 Jun;24(12):5510-20
references.pmid: 15169911
references.citation: J Nutr. 2008 Jun;138(6):1019-24
references.pmid: 18492828
references.citation: Breast Cancer Res. 2009;11(4):R62
references.pmid: 19706193
references.citation: Cancer Res. 2008 Jan 15;68(2):346-51
references.pmid: 18199526
references.citation: J Cell Biol. 1995 Oct;131(1):215-26
references.pmid: 7559778
references.citation: J Cell Biol. 2007 Apr 9;177(1):87-101
references.pmid: 17420292
references.citation: Development. 2011 Dec;138(23):5247-56
references.pmid: 22069192
references.citation: Exp Biol Med (Maywood). 2007 Nov;232(10):1381-90
references.pmid: 17959851
references.citation: Clin Cancer Res. 2011 Aug 15;17(16):5275-86
references.pmid: 21712446
references.citation: J Mammary Gland Biol Neoplasia. 2010 Sep;15(3):291-9
references.pmid: 20824492
references.citation: Stem Cell Rev Rep. 2012 Mar;8(1):1-15
references.pmid: 21537995
references.citation: Nat Med. 2008 Dec;14(12):1384-9
references.pmid: 19029987
references.citation: Phytother Res. 2009 Nov;23(11):1626-33
references.pmid: 19548254
references.citation: Int J Cell Biol. 2011;2011:451676
references.pmid: 21647291
references.citation: BMC Cell Biol. 2012 Jun 14;13:14
references.pmid: 22698263
references.citation: Histochem Cell Biol. 2006 Jul;126(1):35-43
references.pmid: 16456652
references.citation: Curr Biol. 2008 Jul 22;18(14):1094-9
references.pmid: 18635350
references.citation: J Mammary Gland Biol Neoplasia. 1999 Jan;4(1):3-8
references.pmid: 10219902
references.citation: Nature. 2006 Feb 23;439(7079):993-7
references.pmid: 16395311
references.citation: Proc Natl Acad Sci U S A. 2008 Sep 30;105(39):14891-6
references.pmid: 18809919
references.citation: Oncology. 2009;77(6):400-10
references.pmid: 20130423
references.citation: Breast Cancer Res. 2006;8(1):R7
references.pmid: 16417656
references.citation: J Clin Invest. 2007 Dec;117(12):3988-4002
references.pmid: 18060036
references.citation: Proc Natl Acad Sci U S A. 2007 Mar 6;104(10):3871-6
references.pmid: 17360445
references.citation: BMC Biol. 2009 Sep 28;7:63
references.pmid: 19785743
references.citation: Clin Cancer Res. 2013 Feb 1;19(3):643-56
references.pmid: 23149820
references.citation: Dev Biol. 2006 May 15;293(2):414-25
references.pmid: 16545360
references.citation: J Pathol. 2009 Jan;217(2):229-41
references.pmid: 19009588
references.citation: Proc Natl Acad Sci U S A. 2004 Nov 30;101(48):16801-6
references.pmid: 15556998
references.citation: PLoS One. 2010 Oct 19;5(10):e13372
references.pmid: 20976049
references.citation: Proc Nutr Soc. 1994 Mar;53(1):103-11
references.pmid: 8029219
references.citation: Domest Anim Endocrinol. 1989 Oct;6(4):311-9
references.pmid: 2620503
references.citation: Development. 1998 May;125(10):1921-30
references.pmid: 9550724
references.citation: Biostatistics. 2003 Jul;4(3):465-77
references.pmid: 12925512
references.citation: Stem Cell Rev. 2007 Jun;3(2):137-46
references.pmid: 17873346
references.citation: Nat Med. 2012 Sep;18(9):1350-8
references.pmid: 22902876
references.citation: Stem Cells. 2004;22(1):12-20
references.pmid: 14688387
references.citation: Tissue Eng Part A. 2013 Sep;19(17-18):2014-23
references.pmid: 23627869
references.citation: Dev Biol. 2002 May 1;245(1):42-56
references.pmid: 11969254
references.citation: J Biol Chem. 2011 Nov 11;286(45):39297-306
references.pmid: 21914800
references.citation: Exp Biol Med (Maywood). 2009 Apr;234(4):475-82
references.pmid: 19176874
references.citation: BMC Genomics. 2008 Jul 31;9:366
references.pmid: 18671863
references.citation: Cancer Prev Res (Phila). 2012 Jan;5(1):51-60
references.pmid: 21955520
references.citation: Mol Cell Biol. 2013 Mar;33(6):1198-209
references.pmid: 23319049
references.citation: Nature. 2006 Jan 5;439(7072):84-8
references.pmid: 16397499
references.citation: J Anim Sci. 2012 May;90(5):1666-73
references.pmid: 22100590
references.citation: Eur J Cell Biol. 2009 Feb;88(2):117-30
references.pmid: 19013662
references.citation: Cancer Res. 2011 Jan 15;71(2):614-24
references.pmid: 21224357
references.citation: Dev Biol. 2010 Aug 15;344(2):968-78
references.pmid: 20599899
references.citation: J Mammary Gland Biol Neoplasia. 1999 Jan;4(1):53-68
references.pmid: 10219906
references.citation: Cancer Res. 2003 Nov 1;63(21):7098-105
references.pmid: 14612502
references.citation: Mol Endocrinol. 2002 Dec;16(12):2902-12
references.pmid: 12456808