title: PSA-PSMA profiles and their impact on sera PSA levels and angiogenic activity in hyperplasia and human prostate cancer.
abstract: OBJECTIVE
  The relevance of prostate specific antigen (PSA)-prostate specific membrane antigen (PSMA) profiles in pathologic prostate (hyperplasia and cancer) has not been fully understood. The aim of this study is to investigate the impact of PSA-PSMA profiles on sera PSA levels and angiogenic activity in benign prostate hyperplasia (BPH) and prostate carcinoma (PC).
  
  
  METHODS
  The study has been carried out in 6 normal prostate (NP), 29 BPH and 33 PC with dominant Gleason grade>8. Immunohistochemical analysis has been performed. Monoclonal antibodies 3E6 and ER-PR8 have been used to assess PSMA and PSA expression respectively. The evaluation of angiogenesis has been made by CD34 immune marker. Serum levels of PSA have been assayed by Immulite autoanalyser.
  
  
  RESULTS
  The study of each protein separately among sera PSA levels showed that PSMA expression and angiogenic activity have the highest intensity in PC patients with serum PSA levels>20 ng/mL. Nevertheless, the lowest tissue PSA expression was found in PC patients with this latter sera PSA group. The most relevant results showed that in PC patients (PSA+, PSMA+) and (PSA-, PSMA+) profile were found to be inversely related to sera PSA levels. In PC patients, a high immunoexpression of (PSA+, PSMA+) profile has detected in the sera PSA group>20 ng/mL; whereas a high immunoexpression of (PSA-, PSMA+) profile was detected in the sera PSA group between 0 and 4 ng/mL. The highest angiogenic activity was found in PC patients with (PSA+, PSMA+) profile.
  
  
  CONCLUSIONS
  Our findings clearly have supported the feasibility of PSA-PSMA profiles to improve in vivo diagnostic and therapeutic approaches in prostate cancer patients.
authors.affiliation: Unit of immunology and microbiology environmental and carcinogenesis (IMEC), faculty of sciences of Bizerte, university of Carthage, Jarzouna - Bizerte 7021, Tunisia.
authors.forename: A
authors.initials: A
authors.lastname: Ben Jemaa
authors.affiliation: Unit of immunology and microbiology environmental and carcinogenesis (IMEC), faculty of sciences of Bizerte, university of Carthage, Jarzouna - Bizerte 7021, Tunisia.
authors.forename: Y
authors.initials: Y
authors.lastname: Bouraoui
authors.affiliation: Department of urology, hospital of La Rabta, La Rabta Jebbari, 1007 Tunis, Tunisia.
authors.forename: S
authors.initials: S
authors.lastname: Sallami
authors.affiliation: Department of legal medicine, hospital of Charles-Nicolle, boulevard du 9-Avril-1938, Bab Saâdoun, 1007 Tunis, Tunisia.
authors.forename: A
authors.initials: A
authors.lastname: Banasr
authors.affiliation: Department of urology, hospital of La Rabta, La Rabta Jebbari, 1007 Tunis, Tunisia.
authors.forename: Y
authors.initials: Y
authors.lastname: Nouira
authors.affiliation: Unit of immunology and microbiology environmental and carcinogenesis (IMEC), faculty of sciences of Bizerte, university of Carthage, Jarzouna - Bizerte 7021, Tunisia. Electronic address: oueslatiridha12@hotmail.fr.
authors.forename: R
authors.initials: R
authors.lastname: Oueslati
mesh_terms: D000230:Adenocarcinoma / Q000097:blood / Q000098:blood supply / Q000737:chemistry* / Q000201:enzymology / Q000601:surgery / Q000648:ultrastructure; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018952:Antigens, CD34 / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis*; D002451:Cell Compartmentation; D002462:Cell Membrane / Q000201:enzymology; D003593:Cytoplasm / Q000737:chemistry; D004847:Epithelial Cells / Q000737:chemistry / Q000201:enzymology / Q000648:ultrastructure; D005240:Feasibility Studies; D043425:Glutamate Carboxypeptidase II / Q000032:analysis*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009389:Neovascularization, Pathologic / Q000097:blood / Q000378:metabolism* / Q000473:pathology; D011467:Prostate / Q000737:chemistry* / Q000201:enzymology / Q000648:ultrastructure; D017430:Prostate-Specific Antigen / Q000032:analysis* / Q000097:blood; D011468:Prostatectomy; D011470:Prostatic Hyperplasia / Q000097:blood / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D011471:Prostatic Neoplasms / Q000097:blood / Q000098:blood supply / Q000737:chemistry* / Q000201:enzymology / Q000601:surgery / Q000648:ultrastructure; D020728:Transurethral Resection of Prostate; D055815:Young Adult
chemical_list: D018952:Antigens, CD34; D000954:Antigens, Surface; C473848:FOLH1 protein, human; D043425:Glutamate Carboxypeptidase II; D017430:Prostate-Specific Antigen
keywords: Activité angiogénique; Angiogenic activity; BPH; CaP; HBP; PC; PSA; PSMA; Therapy; Traitement