title: Gene expression profiles in peripheral blood mononuclear cells of Asian obstructive sleep apnea patients.
abstract: BACKGROUND
  Obstructive sleep apnea (OSA) syndrome is a complicated disease involving several pathogenic mechanisms. Microarray technology provides a high-throughput means of identifying differentially expressed genes in patients with OSA.
  
  
  METHODS
  An Affymetrix U133A gene chip was used to globally analyze the transcriptome of peripheral blood mononuclear cells (PBMC) in patients with OSA and nonapneic control participants after overnight polysomnography.
  
  
  RESULTS
  Several genes were differentially expressed in patients with OSA compared with control participants. These genes included disintegrin and metalloproteinase domain 29 (ADAM29), solute carrier family 18 (vesicular acetylcholine) member 3 (SLC18A3), cyclin-dependent kinase inhibitor 2C (CDKN2C), and fibronectin-like domain-containing leucine-rich transmembrane protein 2 (FLRT2). Among these genes, the expression levels of ADAM29 (p = 0.00003), FLRT2 (p < 0.0001), and SLC18A3 (p = 0.0006) were found to be possible markers of severe OSA [respiratory disturbance index (RDI) > 30].
  
  
  CONCLUSIONS
  Our data suggest that PCMC expression of ADAM29, FLRT2, and SLC18A3 could be assessed as part of a routine screen to help identify individuals at risk of severe OSA in Asian populations.
authors.forename: Shih-Wei
authors.initials: SW
authors.lastname: Lin
authors.forename: Chi-Neu
authors.initials: CN
authors.lastname: Tsai
authors.forename: Yun-Shien
authors.initials: YS
authors.lastname: Lee
authors.forename: Shu-Fen
authors.initials: SF
authors.lastname: Chu
authors.affiliation: Department of Pulmonary Medicine, Sleep Center, Chang Gung Memorial Hospital at Linkou, Chang Gung University College of Medicine; Chang Gung Institute of Technology, Taoyuan, Taiwan.
authors.forename: Ning-Hung
authors.initials: NH
authors.lastname: Chen
mesh_terms: D051722:ADAM Proteins / Q000235:genetics*; D000328:Adult; D000368:Aged; D044466:Asian People; D015415:Biomarkers / Q000032:analysis; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism*; D008297:Male; D008562:Membrane Glycoproteins; D008565:Membrane Proteins / Q000235:genetics*; D008875:Middle Aged; D012307:Risk Factors; D020181:Sleep Apnea, Obstructive / Q000175:diagnosis / Q000235:genetics*; D059467:Transcriptome / Q000235:genetics*; D050494:Vesicular Acetylcholine Transport Proteins / Q000235:genetics*
chemical_list: D015415:Biomarkers; C404491:FLRT2 protein, human; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; C493459:SLC18A3 protein, human; D050494:Vesicular Acetylcholine Transport Proteins; D051722:ADAM Proteins; C122189:ADAM29 protein, human