title: Bone marrow mesenchymal stem cells from patients with aplastic anemia maintain functional and immune properties and do not contribute to the pathogenesis of the disease.
abstract: Aplastic anemia is a life-threatening bone marrow failure disorder characterized by peripheral pancytopenia and marrow hypoplasia. The majority of cases of aplastic anemia remain idiopathic, although hematopoietic stem cell deficiency and impaired immune responses are hallmarks underlying the bone marrow failure in this condition. Mesenchymal stem/stromal cells constitute an essential component of the bone marrow hematopoietic microenvironment because of their immunomodulatory properties and their ability to support hematopoiesis, and they have been involved in the pathogenesis of several hematologic malignancies. We investigated whether bone marrow mesenchymal stem cells contribute, directly or indirectly, to the pathogenesis of aplastic anemia. We found that mesenchymal stem cell cultures can be established from the bone marrow of aplastic anemia patients and display the same phenotype and differentiation potential as their counterparts from normal bone marrow. Mesenchymal stem cells from aplastic anemia patients support the in vitro homeostasis and the in vivo repopulating function of CD34(+) cells, and maintain their immunosuppressive and anti-inflammatory properties. These data demonstrate that bone marrow mesenchymal stem cells from patients with aplastic anemia do not have impaired functional and immunological properties, suggesting that they do not contribute to the pathogenesis of the disease.
authors.affiliation: Josep Carreras Leukemia Research Institute, Cell Therapy Program of the University of Barcelona, Faculty of Medicine, Barcelona, Spain.
authors.forename: Clara
authors.initials: C
authors.lastname: Bueno
authors.affiliation: GENYO, Centre for Genomics and Oncological Research: Pfizer/University of Granada/Andalusian Regional Government, Granada, Spain.
authors.forename: Mar
authors.initials: M
authors.lastname: Roldan
authors.affiliation: Servicio de Hematología, Hospital Clínico San Carlos, Madrid, Spain.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Anguita
authors.affiliation: Josep Carreras Leukemia Research Institute, Cell Therapy Program of the University of Barcelona, Faculty of Medicine, Barcelona, Spain.
authors.forename: Damia
authors.initials: D
authors.lastname: Romero-Moya
authors.affiliation: Josep Carreras Leukemia Research Institute, Cell Therapy Program of the University of Barcelona, Faculty of Medicine, Barcelona, Spain.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Martín-Antonio
authors.affiliation: Biologics and Genetic Therapies Directorate, Health Products and Food Branch, Health Canada, Ottawa, Ontario, Canada.
authors.forename: Michael
authors.initials: M
authors.lastname: Rosu-Myles
authors.affiliation: Department of Hematology, University Hospital of Salamanca and Institute of Biomedical Research of Salamanca (IBSAL), Salamanca, Spain.
authors.forename: Consuelo
authors.initials: C
authors.lastname: del Cañizo
authors.affiliation: Department of Neurology, Neurovascular Area, Clinical Neurosciences Research Laboratory, Hospital Clínico-Health Research Institute of Santiago de Compostela, Spain.
authors.forename: Francisco
authors.initials: F
authors.lastname: Campos
authors.affiliation: Servicio de Hematología, Hospital Clínico de Málaga, Málaga, Spain.
authors.forename: Regina
authors.initials: R
authors.lastname: García
authors.affiliation: Servicio de Hematología, Hospital Universitario Insular Materno-Infantil, Las Palmas de Gran Canaria, Spain.
authors.forename: Maite
authors.initials: M
authors.lastname: Gómez-Casares
authors.affiliation: Sección de Oncohematología Pediátrica, Hospital Virgen de Arrixaca, Murcia, Spain.
authors.forename: Jose Luis
authors.initials: JL
authors.lastname: Fuster
authors.affiliation: Servicio de Hematología, Hospital Universitario Virgen de las Nieves, Granada, Spain.
authors.forename: Manuel
authors.initials: M
authors.lastname: Jurado
authors.affiliation: Instituto de Parasitología y Biomedicina López-Neyra, CSIC, Granada, Spain.
authors.forename: Mario
authors.initials: M
authors.lastname: Delgado
authors.affiliation: Josep Carreras Leukemia Research Institute, Cell Therapy Program of the University of Barcelona, Faculty of Medicine, Barcelona, Spain Instituciò Catalana de Reserca i Estudis Avançats (ICREA), Barcellona, Spain pmenendez@carrerasresearch.org.
authors.forename: Pablo
authors.initials: P
authors.lastname: Menendez
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000741:Anemia, Aplastic / Q000175:diagnosis / Q000209:etiology / Q000276:immunology* / Q000378:metabolism*; D018952:Antigens, CD34 / Q000378:metabolism; D016022:Case-Control Studies; D002454:Cell Differentiation; D002478:Cells, Cultured; D002648:Child; D018920:Coculture Techniques; D005260:Female; D005312:Fetal Blood / Q000166:cytology; D006085:Graft Survival; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000378:metabolism; D006801:Humans; D056747:Immunomodulation; D016130:Immunophenotyping; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000276:immunology* / Q000378:metabolism*; D008875:Middle Aged; D010641:Phenotype; D055815:Young Adult
chemical_list: D018952:Antigens, CD34
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