title: Protein-centric N-glycoproteomics analysis of membrane and plasma membrane proteins.
abstract: The advent of proteomics technology has transformed our understanding of biological membranes. The challenges for studying membrane proteins have inspired the development of many analytical and bioanalytical tools, and the techniques of glycoproteomics have emerged as an effective means to enrich and characterize membrane and plasma-membrane proteomes. This Review summarizes the development of various glycoproteomics techniques to overcome the hurdles formed by the unique structures and behaviors of membrane proteins with a focus on N-glycoproteomics. Example contributions of N-glycoproteomics to the understanding of membrane biology are provided, and the areas that require future technical breakthroughs are discussed.
authors.affiliation: Department of Chemistry, Simon Fraser University , 8888 University Drive, Burnaby, British Columbia V5A1S6, Canada.
authors.forename: Bingyun
authors.initials: B
authors.lastname: Sun
authors.forename: Leroy
authors.initials: L
authors.lastname: Hood
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000378:metabolism*; D006031:Glycosylation; D006801:Humans; D008562:Membrane Glycoproteins / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D020543:Proteome / Q000378:metabolism; D040901:Proteomics
chemical_list: D008562:Membrane Glycoproteins; D020543:Proteome
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