title: Identification of novel predictive markers for the prognosis of pancreatic ductal adenocarcinoma.
abstract: This study demonstrated that klotho gene expression was significantly lower whereas miR-504 and phospho-IGF-1R levels were significantly higher in PDAC than in normal pancreatic tissues. miR-504 level significantly correlated with klotho mRNA and promoter methylation level in PDACs. Loss of Klotho protein expression, klotho promoter hypermethylation, high miR-504 levels, and high phospho-IGF-1R levels significantly correlated with poor survival, high clinical and pathological stages in PDAC patients. The demethylation reagent and miR-504 inhibitor increased klotho gene expression, invasion, and migration in BxPC-3 and Panc-1 cells. Klotho is a tumor suppressor whose expression in PDAC correlated with progression and prognosis of PDAC.
authors.affiliation: Department of Pathology, Third Xiangya Hospital, Central South University, Changsha, P.R China,1.
authors.forename: Bing
authors.initials: B
authors.lastname: Jiang
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Gu
authors.forename: Yuxiang
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D000368:Aged; D014408:Biomarkers, Tumor / Q000096:biosynthesis / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D005966:Glucuronidase / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D000090265:Klotho Proteins; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics*; D008875:Middle Aged; D010183:Pancreatic Ducts / Q000378:metabolism / Q000473:pathology; D010190:Pancreatic Neoplasms / Q000235:genetics* / Q000473:pathology; D011379:Prognosis; D011401:Promoter Regions, Genetic; D017526:Receptor, IGF Type 1 / Q000096:biosynthesis / Q000235:genetics*; D016019:Survival Analysis
chemical_list: D014408:Biomarkers, Tumor; C550379:MIRN504 microRNA, human; D035683:MicroRNAs; D017526:Receptor, IGF Type 1; D005966:Glucuronidase; D000090265:Klotho Proteins
keywords: IGF-1 receptor; Pancreatic ductal adenocarcinoma; klotho; methylation; miR-504; prognosis