title: Seasonal variation of the chemical composition and antimicrobial and cytotoxic activities of the essential oils from Inga laurina (Sw.) Willd.
abstract: The seasonal chemical composition of essential oils from Inga laurina was determined by GC/MS. In the stem bark's essential oil extracted during the dry season, the presence of terpenoids (30.05%) stood out, and phytol (9.76%) was the major compound identified. For the stem bark oil obtained during the rainy season, in addition to terpenoids (26.63%), a large amount of fatty acids (46.84%) were identified, in particular palmitic acid (25.40%). Regarding the leaves' essential oil obtained in the dry season, esters (42.35%) were the main components. The main ester present was (Z)-hex-3-enyl benzoate (10.15%) and the major compound of this oil was (Z)-hex-3-en-1-ol (14.23%). Terpenoids (33.84%), long-chain alkanes (27.04%) and fatty acids (21.72%) were the main components of the essential oil from leaves in the rainy season. Phytol (33.21%), nonacosane (21.95%) and palmitic acid (15.20%) were the major compounds identified. The antimicrobial activity against aerobic and anaerobic oral bacteria was evaluated by the microdilution broth method and cytotoxic activity was carried out with Vero cells. The essential oils from the rainy season showed a better inhibition of the bacterial growth with Minimal Inhibitory Concentrations (MIC) values of 25 or 50 µg·mL⁻¹ for aerobic bacteria, and high selectivity against bacteria was observed. The large amount of fatty acids in rainy season oils may be related to the better inhibitory effects observed.
authors.affiliation: Laboratory of Natural Products and Chromatography, Chemistry Institute, Federal University of Uberlândia, Uberlândia, Minas Gerais 38408-144, Brazil. fabisbarcelos@hotmail.com.
authors.forename: Fabiana B
authors.initials: FB
authors.lastname: Furtado
authors.affiliation: Laboratory of Natural Products and Chromatography, Chemistry Institute, Federal University of Uberlândia, Uberlândia, Minas Gerais 38408-144, Brazil. aquino@iqufu.ufu.br.
authors.forename: Francisco J T
authors.initials: FJ
authors.lastname: de Aquino
authors.affiliation: Laboratory of Natural Products and Chromatography, Chemistry Institute, Federal University of Uberlândia, Uberlândia, Minas Gerais 38408-144, Brazil. eanascimento@ufu.br.
authors.forename: Evandro A
authors.initials: EA
authors.lastname: Nascimento
authors.affiliation: Federal Institute Goiano, Morrinhos, Goiás 75650-000, Brazil. carla.martins@ifgoiano.edu.br.
authors.forename: Carla
authors.initials: C
authors.lastname: de M Martins
authors.affiliation: Laboratory of Natural Products and Chromatography, Chemistry Institute, Federal University of Uberlândia, Uberlândia, Minas Gerais 38408-144, Brazil. salemos@ufu.br.
authors.forename: Sérgio A L
authors.initials: SA
authors.lastname: de Morais
authors.affiliation: Laboratory of Natural Products and Chromatography, Chemistry Institute, Federal University of Uberlândia, Uberlândia, Minas Gerais 38408-144, Brazil. chang@iqufu.ufu.br.
authors.forename: Roberto
authors.initials: R
authors.lastname: Chang
authors.affiliation: Laboratory of Natural Products and Chromatography, Chemistry Institute, Federal University of Uberlândia, Uberlândia, Minas Gerais 38408-144, Brazil. luisscunha@gmail.com.
authors.forename: Luís C S
authors.initials: LC
authors.lastname: Cunha
authors.affiliation: Nucleus of Research in Sciences and Technology, Laboratory of Research in Applied Microbiology (LaPeMA), University of Franca, Franca, São Paulo 14404-600, Brazil. luis.fernando.leandro@hotmail.com.
authors.forename: Luís F
authors.initials: LF
authors.lastname: Leandro
authors.affiliation: Nucleus of Research in Sciences and Technology, Laboratory of Research in Applied Microbiology (LaPeMA), University of Franca, Franca, São Paulo 14404-600, Brazil. carlos.martins@unifran.edu.br.
authors.forename: Carlos H G
authors.initials: CH
authors.lastname: Martins
authors.affiliation: Laboratory of Natural Products and Chromatography, Chemistry Institute, Federal University of Uberlândia, Uberlândia, Minas Gerais 38408-144, Brazil. mariomm1988@yahoo.com.br.
authors.forename: Mário M
authors.initials: MM
authors.lastname: Martins
authors.affiliation: Institute of Biomedical Sciences, Laboratory of Trypanosomatids, Federal University of Uberlândia, Uberlândia, Minas Gerais 38400-902, Brazil. silva_cv@yahoo.com.br.
authors.forename: Claudio V
authors.initials: CV
authors.lastname: da Silva
authors.affiliation: Institute of Biomedical Sciences, Laboratory of Trypanosomatids, Federal University of Uberlândia, Uberlândia, Minas Gerais 38400-902, Brazil. fabriciomachado@live.com.
authors.forename: Fabrício C
authors.initials: FC
authors.lastname: Machado
authors.affiliation: Laboratory of Natural Products and Chromatography, Chemistry Institute, Federal University of Uberlândia, Uberlândia, Minas Gerais 38408-144, Brazil. alberto@iqufu.ufu.br.
authors.forename: Alberto
authors.initials: A
authors.lastname: de Oliveira
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry* / Q000494:pharmacology; D001420:Bacteria, Aerobic / Q000187:drug effects / Q000254:growth & development; D001421:Bacteria, Anaerobic / Q000187:drug effects / Q000254:growth & development; D002470:Cell Survival / Q000187:drug effects; D002522:Chlorocebus aethiops; D003603:Cytotoxins / Q000737:chemistry* / Q000494:pharmacology; D004952:Esters; D007887:Fabaceae / Q000737:chemistry*; D005227:Fatty Acids / Q000145:classification / Q000302:isolation & purification; D009822:Oils, Volatile / Q000737:chemistry* / Q000494:pharmacology; D009928:Organ Specificity; D024301:Plant Bark / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D010938:Plant Oils / Q000737:chemistry* / Q000494:pharmacology; D012621:Seasons; D013729:Terpenes / Q000145:classification / Q000302:isolation & purification; D014709:Vero Cells
chemical_list: D000890:Anti-Infective Agents; D003603:Cytotoxins; D004952:Esters; D005227:Fatty Acids; D009822:Oils, Volatile; D010938:Plant Oils; D013729:Terpenes
references.citation: Biochimie. 2010 Nov;92(11):1667-73
references.pmid: 20363284
references.citation: J Ethnopharmacol. 2001 Jun;76(1):65-71
references.pmid: 11378283
references.citation: Phytochemistry. 2007 Apr;68(8):1200-5
references.pmid: 17408709
references.citation: Ann Bot. 2002 Oct;90(4):453-60
references.pmid: 12324268
references.citation: Indian J Pharm Sci. 2008 Mar-Apr;70(2):238-41
references.pmid: 20046722
references.citation: Appl Microbiol Biotechnol. 2010 Feb;85(6):1629-42
references.pmid: 19956944
references.citation: Int J Food Microbiol. 1997 Jul 22;37(2-3):155-62
references.pmid: 9310850
references.citation: J Ethnopharmacol. 2006 Jun 15;106(1):82-9
references.pmid: 16423480
references.citation: Molecules. 2011 Jan 13;16(1):543-51
references.pmid: 21233793
references.citation: Phytother Res. 2007 Apr;21(4):308-23
references.pmid: 17199238
references.citation: J Nat Prod. 2007 Jan;70(1):134-6
references.pmid: 17253867
references.citation: Molecules. 2013 Apr 18;18(4):4588-98
references.pmid: 23599016
references.citation: J Appl Microbiol. 2000 Feb;88(2):308-16
references.pmid: 10736000
references.citation: Comp Biochem Physiol C Toxicol Pharmacol. 2012 Nov;156(3-4):148-58
references.pmid: 22885277
references.citation: Braz J Med Biol Res. 2007 Mar;40(3):349-56
references.pmid: 17334532
references.citation: Evid Based Complement Alternat Med. 2012;2012:751435
references.pmid: 22685486
references.citation: Phytochemistry. 2007 Apr;68(8):1104-11
references.pmid: 17363015
references.citation: J Chromatogr. 1963 Aug;11:463-71
references.pmid: 14062605
references.citation: Appl Environ Microbiol. 1992 Feb;58(2):624-9
references.pmid: 1610184
references.citation: Curr Microbiol. 2009 Nov;59(5):554-8
references.pmid: 19688375
references.citation: Molecules. 2013 Jan 14;18(1):963-73
references.pmid: 23344204
references.citation: Proc Natl Acad Sci U S A. 2009 Oct 27;106(43):18073-8
references.pmid: 19805183
references.citation: Parassitologia. 2008 Jun;50(1-2):89-91
references.pmid: 18693566
references.citation: J Ethnopharmacol. 2009 Jul 6;124(1):151-3
references.pmid: 19375495
references.citation: Antimicrob Agents Chemother. 2005 May;49(5):1770-4
references.pmid: 15855494
references.citation: J Chem Ecol. 2001 Oct;27(10):1911-28
references.pmid: 11710601
references.citation: Nat Biotechnol. 1999 Jan;17(1):31-6
references.pmid: 9920265
references.citation: Comp Biochem Physiol B Biochem Mol Biol. 2011 Feb;158(2):164-72
references.pmid: 21094272
references.citation: Phytochemistry. 2004 Feb;65(3):351-8
references.pmid: 14751307
references.citation: J Ethnopharmacol. 2005 Aug 22;100(1-2):80-4
references.pmid: 15964727
references.citation: Oral Microbiol Immunol. 2004 Feb;19(1):61-4
references.pmid: 14678476
references.citation: Parasitol Res. 2006 Jul;99(2):103-7
references.pmid: 16506080
references.citation: Planta Med. 2000 Oct;66(7):672-4
references.pmid: 11105581
references.citation: Phytother Res. 2001 Nov;15(7):638-42
references.pmid: 11746852
references.citation: Molecules. 2010 Aug 10;15(8):5509-24
references.pmid: 20714310
references.citation: Pol J Microbiol. 2009;58(1):61-8
references.pmid: 19469288