title: Effects of minoxidil gel on burn wound healing in rats.
abstract: Minoxidil has been reported to inhibit in-vitro fibroblast proliferation and lysyl hydroxylase activity, a key enzyme in collagen biosynthesis. These in-vitro effects proposed minoxidil to be a potential antifibrotic agent. The present study aimed to investigate the effects of minoxidil gel on wound healing procedure in a second-degree burn model in rats. Wistar rats were anesthetized and a second-degree burn was induced on the back of Wistar rats using a heated 2 cm diameter metal plate. Experimental groups received 2% or 5% topical minoxidil gel, dexpanthenol or sliver sulfadiazine. Histological parameters including collagen content, angiogenesis, number of preserved follicles and necrosis along with tensile strength of burn wound area were assessed on days 3, 7, 14 and 21 post-injury.Microscopic evaluation of specimens collected from sample animals were consistent and showed a second-degree burn. Main histological findings regarding minoxidil topical usage showed that collagen content and tensile strength of burned area did not differ between groups. However, minoxidil increased the number and diameter of blood vessels significantly compared with other groups.Although minoxidil improved the process of wound-healing, our results did not support the proposed idea of its usage as an antifibrotic agent. However, to reject its possible effects as an antifibrotic agent, more objective animal models should be developed and studied.
authors.affiliation: School of Pharmacy and Pharmaceutical Research Center, Kerman University of Medical Sciences, Kerman, Iran.
authors.forename: Payam
authors.initials: P
authors.lastname: Khazaeli
authors.affiliation: Regional Knowledge Hub, and WHO Collaborating Centre for HIV Surveillance, Institute for Futures Studies in Health, Kerman University of Medical Sciences, Kerman, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Karamouzian
authors.affiliation: School of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran.
authors.forename: Shohreh
authors.initials: S
authors.lastname: Rohani
authors.affiliation: Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran .
authors.forename: Behnam
authors.initials: B
authors.lastname: Sadeghirad
authors.affiliation: Research Center for Health Services Management, Institute for Futures Studies in Health, Kerman University of Medical Sciences, Kerman, Iran.
authors.forename: Nima
authors.initials: N
authors.lastname: Ghalekhani
keywords: Angiogenesis; Minoxidil; Second-degree burn; Topical gel; Wound healing
references.citation: J Cell Biol. 1994 Feb;124(4):401-4
references.pmid: 8106541
references.citation: Br J Dermatol. 1998 Mar;138(3):407-11
references.pmid: 9580790
references.citation: Phys Ther. 1995 Jun;75(6):526-38
references.pmid: 7770498
references.citation: J Invest Dermatol. 1984 Jan;82(1):90-3
references.pmid: 6197493
references.citation: J Cardiovasc Pharmacol. 2000 Aug;36(2):270-6
references.pmid: 10942171
references.citation: Br J Plast Surg. 1963 Apr;16:118-24
references.pmid: 14017931
references.citation: J Invest Dermatol. 1992 Dec;99(6):864-9
references.pmid: 1335016
references.citation: Iran J Pharm Res. 2013 Winter;12(1):3-8
references.pmid: 24250565
references.citation: Arch Biochem Biophys. 1994 Jan;308(1):42-7
references.pmid: 8311472
references.citation: Biochem J. 1992 Apr 1;283 ( Pt 1):51-4
references.pmid: 1314568
references.citation: Plast Reconstr Surg. 1992 Apr;89(4):722-30
references.pmid: 1546087
references.citation: Br J Dermatol. 1991 Sep;125(3):217-21
references.pmid: 1911312
references.citation: J Invest Dermatol. 1997 Feb;108(2):205-9
references.pmid: 9008235
references.citation: Burns. 2001 Dec;27(8):817-27
references.pmid: 11718984
references.citation: Br J Plast Surg. 1995 Apr;48(3):154-60
references.pmid: 7735678
references.citation: Burns. 2001 Aug;27(5):517-22
references.pmid: 11451610
references.citation: Invest Ophthalmol Vis Sci. 1994 Feb;35(2):463-9
references.pmid: 8112995
references.citation: Iran J Pharm Res. 2012 Winter;11(1):229-33
references.pmid: 24250444
references.citation: Graefes Arch Clin Exp Ophthalmol. 1995 Jun;233(6):347-53
references.pmid: 7672621
references.citation: Invest Ophthalmol Vis Sci. 1993 Mar;34(3):567-75
references.pmid: 8383644
references.citation: Clin Dermatol. 1988 Oct-Dec;6(4):152-8
references.pmid: 2850865
references.citation: Graefes Arch Clin Exp Ophthalmol. 1994 Aug;232(8):499-502
references.pmid: 7926886
references.citation: Burns. 2009 Feb;35(1):15-29
references.pmid: 18952381
references.citation: Matrix Biol. 2005 Jun;24(4):261-70
references.pmid: 15908192
references.citation: Adv Wound Care. 1997 Mar-Apr;10(2):39-44
references.pmid: 9204810
references.citation: Br J Dermatol. 2004 Feb;150(2):186-94
references.pmid: 14996087
references.citation: Arch Biochem Biophys. 1992 Jan;292(1):234-8
references.pmid: 1309293
references.citation: Arch Dermatol Res. 1995;287(5):506-8
references.pmid: 7542863
references.citation: J Invest Dermatol. 1991 Sep;97(3):534-6
references.pmid: 1875054
references.citation: Dermatologica. 1987;175 Suppl 2:12-8
references.pmid: 2826267
references.citation: Burns. 1993 Jun;19(3):187-91
references.pmid: 8507361
references.citation: Burns. 1991 Dec;17(6):443-51
references.pmid: 1793491
references.citation: J Invest Dermatol. 1984 May;82(5):515-7
references.pmid: 6239893
references.citation: J Biol Chem. 1987 Sep 5;262(25):11973-8
references.pmid: 2442156
references.citation: Biochem Biophys Res Commun. 2003 Aug 8;307(4):803-9
references.pmid: 12878181
references.citation: J Ethnopharmacol. 2000 Jun;70(3):315-21
references.pmid: 10837993
references.citation: Burns. 2009 Jun;35(4):463-75
references.pmid: 18951704