title: Dispersive solid-phase extraction followed by high-performance liquid chromatography/tandem mass spectrometry for the determination of ricinine in cooking oil.
abstract: A rapid and accurate method by liquid chromatography/tandem mass spectrometry (LC-MS/MS) using positive electrospray was established for the determination of ricinine in cooking oils. The homogenized samples, spiked with (13)C6-labelled ricinine as an internal standard, were extracted using ethanol/water (20:80, v/v) and purified by dispersive solid-phase extraction (dSPE) using primary-secondary amine (PSA) and C18 as adsorbents. The extract was separated in a short C18 reversed-phase column using methanol/water (25:75, v/v) as the mobile phase and detected in multiple reaction monitoring (MRM) mode with the absolute matrix effect of 93.2-102.2%. The alkali-metal adduct ions were discussed and the mass/mass fragmentation pathway was explained. Ricinine showed good linearity in the range of 0.5-50.0 μg/kg with the limit of quantitation 0.5 μg/kg. The recoveries were between 86.0% and 98.3% with the intra- and inter-day RSDs of 2.6-7.0%, 5.5-10.8%, respectively. This method could be applied to the rapid quantification of ricinine in cooking oils.
authors.affiliation: School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310035, China.
authors.forename: Meiqiang
authors.initials: M
authors.lastname: Cai
authors.affiliation: Zhejiang Provincial Key Laboratory of Health Risk Appraisal for Trace Toxic Chemicals, Ningbo Municipal Center for Disease Control and Prevention, Ningbo, Zhejiang 315010, China; Ningbo Key Laboratory of Poison Research and Control, Ningbo Municipal Center for Disease Control and Prevention, Ningbo, Zhejiang 315010, China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Chen
authors.affiliation: School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310035, China.
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Wei
authors.affiliation: Zhejiang Provincial Key Laboratory of Health Risk Appraisal for Trace Toxic Chemicals, Ningbo Municipal Center for Disease Control and Prevention, Ningbo, Zhejiang 315010, China; Ningbo Key Laboratory of Poison Research and Control, Ningbo Municipal Center for Disease Control and Prevention, Ningbo, Zhejiang 315010, China.
authors.forename: Shengdong
authors.initials: S
authors.lastname: Pan
authors.affiliation: Zhejiang Provincial Key Laboratory of Health Risk Appraisal for Trace Toxic Chemicals, Ningbo Municipal Center for Disease Control and Prevention, Ningbo, Zhejiang 315010, China; Ningbo Key Laboratory of Poison Research and Control, Ningbo Municipal Center for Disease Control and Prevention, Ningbo, Zhejiang 315010, China.
authors.forename: Yonggang
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Zhejiang Provincial Key Laboratory of Health Risk Appraisal for Trace Toxic Chemicals, Ningbo Municipal Center for Disease Control and Prevention, Ningbo, Zhejiang 315010, China; Ningbo Key Laboratory of Poison Research and Control, Ningbo Municipal Center for Disease Control and Prevention, Ningbo, Zhejiang 315010, China. Electronic address: jmcjc@163.com.
authors.forename: Micong
authors.initials: M
authors.lastname: Jin
mesh_terms: D000470:Alkaloids / Q000737:chemistry* / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005506:Food Contamination / Q000032:analysis; D010938:Plant Oils / Q000737:chemistry*; D011728:Pyridones / Q000737:chemistry* / Q000302:isolation & purification*; D052616:Solid Phase Extraction / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000470:Alkaloids; D010938:Plant Oils; D011728:Pyridones; C019550:ricinine
keywords: Cooking oil; Dispersive solid-phase extraction (dSPE); LC–MS/MS; Ricinine