title: Detection of collagen through FTIR and HPLC from the body and foot of Donax cuneatus Linnaeus, 1758.
abstract: To make more effective use of available marine resources, acid soluble collagen (ASC) was isolated from body and foot of wedge clam Donax cuneatus Linnaeus, 1758 with acetic acid and was characterized for their potential and commercial applications. The yield of ASC was 17% and 23% respectively. SDS PAGE, UV and FTIR spectroscopy showed that both were type I mainly with slight differences. HPLC was used for identifying the presence of different types of amino acids, where glycine was more or less 20% in both the samples and takes the lead amino acid position and presence of imino acids (11.8 and 12.6%) has been the characteristic feature of type I collagen.
authors.affiliation: Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur, 603 203 Chennai, Tamilnadu India.
authors.forename: R A
authors.initials: RA
authors.lastname: Nazeer
authors.affiliation: Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur, 603 203 Chennai, Tamilnadu India.
authors.forename: R
authors.initials: R
authors.lastname: Kavitha
authors.affiliation: Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur, 603 203 Chennai, Tamilnadu India.
authors.forename: R Jai
authors.initials: RJ
authors.lastname: Ganesh
authors.affiliation: Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur, 603 203 Chennai, Tamilnadu India.
authors.forename: Shabeena Yousuf
authors.initials: SY
authors.lastname: Naqash
authors.affiliation: Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur, 603 203 Chennai, Tamilnadu India.
authors.forename: N S Sampath
authors.initials: NS
authors.lastname: Kumar
authors.affiliation: Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur, 603 203 Chennai, Tamilnadu India.
authors.forename: R
authors.initials: R
authors.lastname: Ranjith
keywords: Collagen; Donax cuneatus; FTIR; HPLC; Imino acids
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