title: Directing neuronal signaling through cell-surface glycan engineering.
abstract: The ability to tailor plasma membranes with specific glycans may enable the control of signaling events that are critical for proper development and function. We report a method to modify cell surfaces with specific sulfated chondroitin sulfate (CS) glycosaminoglycans using chemically modified liposomes. Neurons engineered to display CS-E-enriched polysaccharides exhibited increased activation of neurotrophin-mediated signaling pathways and enhanced axonal growth. This approach provides a facile, general route to tailor cell membranes with biologically active glycans and demonstrates the potential to direct important cellular events through cell-surface glycan engineering.
authors.affiliation: Division of Chemistry and Chemical Engineering, California Institute of Technology and Howard Hughes Medical Institute , 1200 East California Boulevard, Pasadena, California 91125, United States.
authors.forename: Abigail
authors.initials: A
authors.lastname: Pulsipher
authors.forename: Matthew E
authors.initials: ME
authors.lastname: Griffin
authors.forename: Shannon E
authors.initials: SE
authors.lastname: Stone
authors.forename: Joshua M
authors.initials: JM
authors.lastname: Brown
authors.forename: Linda C
authors.initials: LC
authors.lastname: Hsieh-Wilson
mesh_terms: D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000737:chemistry* / Q000378:metabolism; D002809:Chondroitin Sulfates / Q000737:chemistry* / Q000378:metabolism; D008081:Liposomes / Q000737:chemistry* / Q000378:metabolism; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D051381:Rats
chemical_list: D008081:Liposomes; D002809:Chondroitin Sulfates
references.citation: Nat Chem Biol. 2012 Jul;8(7):661-8
references.pmid: 22683610
references.citation: Angew Chem Int Ed Engl. 2010 Dec 3;49(49):9417-21
references.pmid: 21031382
references.citation: Proc Natl Acad Sci U S A. 2011 Jun 14;108(24):9747-52
references.pmid: 21628576
references.citation: J Am Chem Soc. 2012 Jun 20;134(24):9914-7
references.pmid: 22646989
references.citation: Proc Natl Acad Sci U S A. 2013 May 7;110(19):7826-31
references.pmid: 23610394
references.citation: J Biol Chem. 2012 Jun 1;287(23):18985-94
references.pmid: 22493510
references.citation: PLoS One. 2013 Aug 19;8(8):e72304
references.pmid: 23977277
references.citation: Nat Neurosci. 2012 Jan 15;15(3):414-22, S1-2
references.pmid: 22246436
references.citation: Proc Natl Acad Sci U S A. 2012 Mar 27;109(13):4768-73
references.pmid: 22411830
references.citation: Nat Chem Biol. 2006 Sep;2(9):467-73
references.pmid: 16878128
references.citation: J Am Chem Soc. 2010 Mar 3;132(8):2614-21
references.pmid: 20131824
references.citation: Science. 2008 May 2;320(5876):664-7
references.pmid: 18451302
references.citation: Cell. 1999 Oct 1;99(1):13-22
references.pmid: 10520990
references.citation: Nature. 2003 May 22;423(6938):443-8
references.pmid: 12761550
references.citation: Bioconjug Chem. 2010 Mar 17;21(3):537-43
references.pmid: 20184308
references.citation: Annu Rev Biochem. 2003;72:609-42
references.pmid: 12676795
references.citation: Angew Chem Int Ed Engl. 2011 Apr 26;50(18):4113-8
references.pmid: 21472942
references.citation: Nat Struct Mol Biol. 2004 Sep;11(9):857-62
references.pmid: 15311269
references.citation: Nat Rev Cancer. 2005 Jul;5(7):526-42
references.pmid: 16069816
references.citation: ChemMedChem. 2008 Sep;3(9):1356-61
references.pmid: 18666266
references.citation: Mol Cell. 2000 Sep;6(3):743-50
references.pmid: 11030354
references.citation: J Neurosci. 2012 Jul 4;32(27):9429-37
references.pmid: 22764251
references.citation: Nat Rev Drug Discov. 2005 Feb;4(2):145-60
references.pmid: 15688077
references.citation: Nat Chem Biol. 2014 Jan;10(1):69-75
references.pmid: 24292068
references.citation: J Am Chem Soc. 2011 Jun 8;133(22):8704-13
references.pmid: 21561150