title: Hepatic HMOX1 expression positively correlates with Bach-1 and miR-122 in patients with HCV mono and HIV/HCV coinfection.
abstract: OBJECTIVE
  To analyze the expression of HMOX1 and miR-122 in liver biopsy samples obtained from HCV mono-and HIV/HCV co-infected patients in relation to selected clinical parameters, histological examination and IL-28B polymorphism as well as to determine whether HMOX1 expression is dependent on Bach-1.
  
  
  METHODS
  The study group consisted of 90 patients with CHC: 69 with HCV mono and 21 with HIV/HCV co-infection. RT-PCR was used in the analysis of HMOX1, Bach-1 and miR-122 expression in liver biopsy samples and in the assessment of IL-28B single-nucleotide polymorphism C/T (rs12979860) in the blood. Moreover in liver biopsy samples an analysis of HO-1 and Bach-1 protein level by Western Blot was performed.
  
  
  RESULTS
  HCV mono-infected patients, with lower grading score (G<2) and higher HCV viral load (>600000 IU/mL) demonstrated higher expression of HMOX1. In patients with HIV/HCV co-infection, the expression of HMOX1 was lower in patients with lower lymphocyte CD4 count and higher HIV viral load. IL28B polymorphism did not affect the expression of either HMOX1 or miR-122. Higher HMOX1 expression correlated with higher expression of Bach-1 (Spearman's ρ = 0.586, p = 0.000001) and miR-122 (Spearman's ρ = 0.270, p = 0.014059).
  
  
  CONCLUSIONS
  HMOX1 and miR-122 play an important role in the pathogenesis of CHC in HCV mono-and HIV/HCV co-infected patients. Reduced expression of HMOX1 in patients with HIV/HCV co-infection may indicate a worse prognosis in this group. Our results do not support the importance of Bach-1 in repression of HMOX1 in patients with chronic hepatitis C.
authors.affiliation: Department of Infectious Diseases and Hepatology, Medical University of Lodz, Łódź, Poland.
authors.forename: Elżbieta
authors.initials: E
authors.lastname: Jabłonowska
authors.affiliation: Department of Infectious Diseases and Hepatology, Medical University of Lodz, Łódź, Poland.
authors.forename: Kamila
authors.initials: K
authors.lastname: Wójcik
authors.affiliation: Central Laboratory, Medical University of Lodz, Łódź, Poland.
authors.forename: Bożena
authors.initials: B
authors.lastname: Szymańska
authors.affiliation: Department of Pathology, Biegański Provincial Specialistic Hospital, Łódź, Poland.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Omulecka
authors.affiliation: Laboratory of Neuroimmunology, Department of Neurology, Medical University of Łódź, Poland.
authors.forename: Hanna
authors.initials: H
authors.lastname: Cwiklińska
authors.affiliation: Department of Infectious Diseases and Hepatology, Medical University of Lodz, Łódź, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Piekarska
mesh_terms: D000328:Adult; D050976:Basic-Leucine Zipper Transcription Factors / Q000235:genetics / Q000378:metabolism*; D018791:CD4 Lymphocyte Count; D060085:Coinfection / Q000150:complications / Q000276:immunology / Q000378:metabolism* / Q000821:virology; D003710:Demography; D051856:Fanconi Anemia Complementation Group Proteins / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015658:HIV Infections / Q000150:complications / Q000276:immunology / Q000378:metabolism* / Q000821:virology; D051547:Heme Oxygenase-1 / Q000235:genetics / Q000378:metabolism*; D019698:Hepatitis C, Chronic / Q000150:complications / Q000276:immunology / Q000378:metabolism* / Q000821:virology; D006801:Humans; D008099:Liver / Q000378:metabolism*; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D019562:Viral Load
chemical_list: C102108:BACH1 protein, human; D050976:Basic-Leucine Zipper Transcription Factors; D051856:Fanconi Anemia Complementation Group Proteins; C531907:MIRN122 microRNA, human; D035683:MicroRNAs; C496541:HMOX1 protein, human; D051547:Heme Oxygenase-1
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