title: [Granulosa cells model transfected by ENO1 overexpression adenovirus vector and the effect of ENO1 overexpression on progesterone secretion].
abstract: OBJECTIVE
  To construct primary cultured granulosa cells model of Zi Gooses tansfected by alpha-enolase (ENO1) overexpression adenovirus vector, and to detect the effect of ENO1 overexpression of granulose cells on progesterone secretion.
  
  
  METHODS
  Granulosa cells were infected with Ad-CMV-ENO1 in gradient multiplicity of infection(MOI) levels:100, 250, 350 and 400 pfu/cell. Twenty four hours and 48 h after infection, green fluorescent protein (GDP) was respectively detected by fluorescence inverted microscopy. The effect of ENO1 overexpression of granulose cells on progesterone secretion was detected by the step double antibody sandwich enzyme-linked immunosorbent assay (ELISA).
  
  
  RESULTS
  The optimal infection rate (100%) was achieved when MOI was 800 pfu/cell,48h after infection. Real time RT-PCR and Western blot showed that the level of mRNA and protein expression ENO1 were increased significantly after infection (P < 0.01); The granulosa cells progesterone secretion of Ad-CMV-ENO1 group increased signigicantly (P < 0.01).
  
  
  CONCLUSIONS
  ENO1 overexpression could make the primary culture follicle granulosa cells in vitro improve progesterone secretion.
authors.forename: Wei-Hong
authors.initials: WH
authors.lastname: Zhang
authors.forename: Hong
authors.initials: H
authors.lastname: Ji
authors.forename: Jiang-Lu
authors.initials: JL
authors.lastname: Wang
authors.forename: Rong-He
authors.initials: RH
authors.lastname: He
authors.forename: Huan-Min
authors.initials: HM
authors.lastname: Yang
mesh_terms: D000256:Adenoviridae; D014408:Biomarkers, Tumor / Q000235:genetics*; D002460:Cell Line; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000235:genetics*; D005260:Female; D005822:Genetic Vectors; D006107:Granulosa Cells / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000235:genetics; D006801:Humans; D006080:Ovarian Follicle / Q000166:cytology; D010751:Phosphopyruvate Hydratase / Q000235:genetics*; D011374:Progesterone / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D014162:Transfection; D025521:Tumor Suppressor Proteins / Q000235:genetics*
chemical_list: D014408:Biomarkers, Tumor; D004268:DNA-Binding Proteins; D012333:RNA, Messenger; D025521:Tumor Suppressor Proteins; D049452:Green Fluorescent Proteins; D011374:Progesterone; C095157:ENO1 protein, human; D010751:Phosphopyruvate Hydratase