title: Characterization of hydrocarbon-degrading and biosurfactant-producing Pseudomonas sp. P-1 strain as a potential tool for bioremediation of petroleum-contaminated soil.
abstract: The Pseudomonas sp. P-1 strain, isolated from heavily petroleum hydrocarbon-contaminated soil, was investigated for its capability to degrade hydrocarbons and produce a biosurfactant. The strain degraded crude oil, fractions A5 and P3 of crude oil, and hexadecane (27, 39, 27 and 13% of hydrocarbons added to culture medium were degraded, respectively) but had no ability to degrade phenanthrene. Additionally, the presence of gene-encoding enzymes responsible for the degradation of alkanes and naphthalene in the genome of the P-1 strain was reported. Positive results of blood agar and methylene blue agar tests, as well as the presence of gene rhl, involved in the biosynthesis of rhamnolipid, confirmed the ability of P-1 for synthesis of glycolipid biosurfactant. 1H and 13C nuclear magnetic resonance, Fourier transform infrared spectrum and mass spectrum analyses indicated that the extracted biosurfactant was affiliated with rhamnolipid. The results of this study indicate that the P-1 and/or biosurfactant produced by this strain have the potential to be used in bioremediation of hydrocarbon-contaminated soils.
authors.affiliation: Department of Microbiology, University of Silesia, Jagiellońska 28, 40-032, Katowice, Poland, mpacwa@us.edu.pl.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Pacwa-Płociniczak
authors.forename: Grażyna Anna
authors.initials: GA
authors.lastname: Płaza
authors.forename: Anna
authors.initials: A
authors.lastname: Poliwoda
authors.forename: Zofia
authors.initials: Z
authors.lastname: Piotrowska-Seget
mesh_terms: D001673:Biodegradation, Environmental; D004787:Environmental Pollution; D052918:Environmental Restoration and Remediation / Q000379:methods*; D006838:Hydrocarbons / Q000032:analysis / Q000378:metabolism*; D010578:Petroleum / Q000032:analysis / Q000378:metabolism*; D011549:Pseudomonas / Q000378:metabolism*; D012987:Soil / Q000737:chemistry; D012989:Soil Pollutants / Q000032:analysis / Q000378:metabolism*; D013501:Surface-Active Agents / Q000032:analysis / Q000378:metabolism*
chemical_list: D006838:Hydrocarbons; D010578:Petroleum; D012987:Soil; D012989:Soil Pollutants; D013501:Surface-Active Agents
references.citation: Biochim Biophys Acta. 1999 Sep 22;1440(2-3):244-52
references.pmid: 10521708
references.citation: Appl Environ Microbiol. 2005 May;71(5):2288-93
references.pmid: 15870313
references.citation: Appl Environ Microbiol. 2001 Jul;67(7):3127-33
references.pmid: 11425732
references.citation: J Microbiol Methods. 2004 Mar;56(3):339-47
references.pmid: 14967225
references.citation: Microb Cell Fact. 2012 Mar 23;11:35
references.pmid: 22443185
references.citation: Colloids Surf B Biointerfaces. 2010 Aug 1;79(1):174-83
references.pmid: 20417068
references.citation: Chemosphere. 2013 May;91(9):1332-7
references.pmid: 23561856
references.citation: Appl Microbiol Biotechnol. 2010 Feb;85(5):1521-31
references.pmid: 19730852
references.citation: Biotechnol Bioeng. 1994 Aug 20;44(5):661-6
references.pmid: 18618803
references.citation: Curr Microbiol. 2004 Sep;49(3):186-91
references.pmid: 15386102
references.citation: Appl Microbiol Biotechnol. 1998 Nov;50(5):520-9
references.pmid: 9866170
references.citation: Int J Mol Sci. 2011 Jan 18;12(1):633-54
references.pmid: 21340005
references.citation: Z Naturforsch C J Biosci. 2006 Mar-Apr;61(3-4):203-12
references.pmid: 16729578
references.citation: Biotechnol Prog. 2005 Sep-Oct;21(5):1562-6
references.pmid: 16209563
references.citation: Biotechnol Prog. 2002 Nov-Dec;18(6):1277-81
references.pmid: 12467462
references.citation: Environ Pollut. 2005 Jan;133(1):71-84
references.pmid: 15327858
references.citation: Appl Microbiol Biotechnol. 2006 Oct;72(6):1322-9
references.pmid: 16804694
references.citation: Appl Biochem Biotechnol. 2012 Mar;166(5):1148-66
references.pmid: 22198867
references.citation: J Bacteriol. 1996 Apr;178(8):2402-8
references.pmid: 8636045
references.citation: Biotechnol Adv. 2010 Jul-Aug;28(4):436-50
references.pmid: 20172021
references.citation: Colloids Surf B Biointerfaces. 2012 Jun 15;95:23-9
references.pmid: 22445235
references.citation: J Environ Biol. 2009 Jan;30(1):161-3
references.pmid: 20112880
references.citation: J Microbiol Methods. 2012 Mar;88(3):348-55
references.pmid: 22245375
references.citation: Sci Total Environ. 2009 Apr 1;407(8):3024-32
references.pmid: 19201450
references.citation: AMB Express. 2011 Mar 28;1(1):5
references.pmid: 21906330
references.citation: Biochim Biophys Acta. 2000 Nov 15;1488(3):211-8
references.pmid: 11082531
references.citation: J Appl Microbiol. 2013 Feb;114(2):373-83
references.pmid: 23164038
references.citation: Biotechnol Res Int. 2011;2011:941810
references.pmid: 21350672
references.citation: Microb Ecol. 2006 Oct;52(3):523-32
references.pmid: 16944337
references.citation: Appl Environ Microbiol. 1987 Feb;53(2):224-9
references.pmid: 16347271
references.citation: Braz J Microbiol. 2010 Jul;41(3):668-75
references.pmid: 24031542
references.citation: World J Microbiol Biotechnol. 1996 Jan;12(1):82-4
references.pmid: 24415095