title: Analysis of global changes in gene expression induced by human polynucleotide phosphorylase (hPNPase(old-35)).
abstract: As a strategy to identify gene expression changes affected by human polynucleotide phosphorylase (hPNPase(old-35)), we performed gene expression analysis of HeLa cells in which hPNPase(old-35) was overexpressed. The observed changes were then compared to those of HO-1 melanoma cells in which hPNPase(old-35) was stably knocked down. Through this analysis, 90 transcripts, which positively or negatively correlated with hPNPase(old-35) expression, were identified. The majority of these genes were associated with cell communication, cell cycle, and chromosomal organization gene ontology categories. For a number of these genes, the positive or negative correlations with hPNPase(old-35) expression were consistent with transcriptional data extracted from the TCGA (The Cancer Genome Atlas) expression datasets for colon adenocarcinoma (COAD), skin cutaneous melanoma (SKCM), ovarian serous cyst adenocarcinoma (OV), and prostate adenocarcinoma (PRAD). Further analysis comparing the gene expression changes between Ad.hPNPase(old-35) infected HO-1 melanoma cells and HeLa cells overexpressing hPNPase(old-35) under the control of a doxycycline-inducible promoter, revealed global changes in genes involved in cell cycle and mitosis. Overall, this study provides further evidence that hPNPase(old-35) is associated with global changes in cell cycle-associated genes and identifies potential gene targets for future investigation.
authors.affiliation: Department of Human and Molecular Genetics, Virginia Commonwealth University, School of Medicine, Richmond, Virginia.
authors.forename: Upneet K
authors.initials: UK
authors.lastname: Sokhi
authors.forename: Manny D
authors.initials: MD
authors.lastname: Bacolod
authors.forename: Luni
authors.initials: L
authors.lastname: Emdad
authors.forename: Swadesh K
authors.initials: SK
authors.lastname: Das
authors.forename: Catherine I
authors.initials: CI
authors.lastname: Dumur
authors.forename: Michael F
authors.initials: MF
authors.lastname: Miles
authors.forename: Devanand
authors.initials: D
authors.lastname: Sarkar
authors.forename: Paul B
authors.initials: PB
authors.lastname: Fisher
mesh_terms: D017209:Apoptosis / Q000235:genetics; D002453:Cell Cycle / Q000235:genetics*; D005095:Exoribonucleases / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D008545:Melanoma / Q000235:genetics* / Q000473:pathology; D011401:Promoter Regions, Genetic; D012878:Skin Neoplasms; D000096142:Cutaneous Malignant Melanoma; D024101:Mitochondrial Proteins
chemical_list: D005095:Exoribonucleases; C476417:PNPT1 protein, human; D024101:Mitochondrial Proteins
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