title: Quorum sensing activity of Hafnia alvei isolated from packed food.
abstract: Quorum sensing (QS) is a mechanism adopted by bacteria to regulate expression of genes according to population density. N-acylhomoserine lactones (AHLs) are a type of QS signalling molecules commonly found in Gram-negative bacteria which have been reported to play a role in microbial spoilage of foods and pathogenesis. In this study, we isolated an AHL-producing Hafnia alvei strain (FB1) from spherical fish pastes. Analysis via high resolution triple quadrupole liquid chromatography/mass spectrometry (LC/MS) on extracts from the spent supernatant of H. alvei FB1 revealed the existence of two short chain AHLs: N-(3-oxohexanoyl) homoserine lactone (3-oxo-C6-HSL) and N-(3-oxo- octanoyl) homoserine lactone (3-oxo-C8-HSL). To our knowledge, this is the first report of the production of AHLs, especially 3-oxo-C8-HSL, by H. alvei.
authors.affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia. chloe_tjy@yahoo.com.
authors.forename: Jia-Yi
authors.initials: JY
authors.lastname: Tan
authors.affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia. yinwaifong@yahoo.com.
authors.forename: Wai-Fong
authors.initials: WF
authors.lastname: Yin
authors.affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia. kokgan@um.edu.my.
authors.forename: Kok-Gan
authors.initials: KG
authors.lastname: Chan
mesh_terms: D054742:Acyl-Butyrolactones / Q000378:metabolism; D000818:Animals; D002853:Chromatography, Liquid; D002855:Chromatography, Thin Layer; D004275:DNA, Ribosomal / Q000235:genetics; D005399:Fishes; D018857:Food Packaging; D020611:Hafnia alvei / Q000302:isolation & purification* / Q000502:physiology*; D013058:Mass Spectrometry; D010802:Phylogeny; D053038:Quorum Sensing; D012015:Reference Standards
chemical_list: D054742:Acyl-Butyrolactones; D004275:DNA, Ribosomal
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