title: Distribution of Capnocytophaga canimorsus in dogs and cats with genetic characterization of isolates.
abstract: Capnocytophaga canimorsus, which is often found in the oral cavities of dogs and cats, is sometimes transmitted to humans, causing severe infection. To elucidate the risk of C. canimorsus in humans and animals, this study was undertaken to characterize this bacterium epidemiologically and genetically. We examined the distribution of C. canimorsus in dogs and cats, and analyzed the correlation between the presence of bacteria and individual factors statistically. We also compared C. canimorsus isolates genetically using 16S rRNA gene sequence analysis and pulsed-field gel electrophoresis (PFGE). C. canimorsus was detected in 76 of 109 dogs (69.7%) and 57 of 104 cats (54.8%). A relation between C. canimorsus presence and some individual factors was detected both in dogs and cats, but the predictive factors of carrying the bacterium differed between dogs and cats. 16S rRNA gene sequences from C. canimorsus isolates in this study were combined with previously published sequences to assess their intra-specific phylogeny. Results show that C. canimorsus is classifiable into two main groups (I and II) with differing γ-glutamyl aminopeptidase activity. Strains from human patients belonged unevenly to group I, possibility suggesting that group I can be transmitted to humans and group II is indigenous only to the oral cavities of dogs and cats. PFGE genotyping showed high discriminatory power, and the dendrogram accorded with genetic segregation between isolates of group I and II. Sma I-digest PFGE developed for this study is useful as a molecular typing method for additional epidemiological and phylogenetic studies of C. canimorsus.
authors.affiliation: Department of Microbiology, Osaka City Institute of Public Health and Environmental Sciences, 8-34 Tojo-cho, Tennoji-ku, Osaka 543-0026, Japan. Electronic address: kaor-umeda@city.osaka.lg.jp.
authors.forename: Kaoru
authors.initials: K
authors.lastname: Umeda
authors.affiliation: Osaka Municipal Animal Care and Control Center, 2-5-74, Shibatani, Suminoe-ku, Osaka 559-0021, Japan.
authors.forename: Risa
authors.initials: R
authors.lastname: Hatakeyama
authors.affiliation: Osaka Municipal Animal Care and Control Center, 2-5-74, Shibatani, Suminoe-ku, Osaka 559-0021, Japan.
authors.forename: Takuto
authors.initials: T
authors.lastname: Abe
authors.affiliation: Department of Microbiology, Osaka City Institute of Public Health and Environmental Sciences, 8-34 Tojo-cho, Tennoji-ku, Osaka 543-0026, Japan.
authors.forename: Koh-ichi
authors.initials: K
authors.lastname: Takakura
authors.affiliation: Department of International Health, Institute of Tropical Medicine, Nagasaki University, Nagasaki, 1-12-4, Sakamoto, Nagasaki-shi, Nagasaki 852-8523, Japan.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Wada
authors.affiliation: Department of Microbiology, Osaka City Institute of Public Health and Environmental Sciences, 8-34 Tojo-cho, Tennoji-ku, Osaka 543-0026, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Ogasawara
authors.affiliation: Osaka Municipal Animal Care and Control Center, 2-5-74, Shibatani, Suminoe-ku, Osaka 559-0021, Japan.
authors.forename: Shu-ichi
authors.initials: S
authors.lastname: Sanada
authors.affiliation: Department of Microbiology, Osaka City Institute of Public Health and Environmental Sciences, 8-34 Tojo-cho, Tennoji-ku, Osaka 543-0026, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Hase
mesh_terms: D000818:Animals; D002206:Capnocytophaga / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D002371:Cat Diseases / Q000453:epidemiology / Q000382:microbiology*; D002415:Cats; D004283:Dog Diseases / Q000453:epidemiology / Q000382:microbiology*; D004285:Dogs; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D005838:Genotype; D016905:Gram-Negative Bacterial Infections / Q000453:epidemiology / Q000382:microbiology*; D006801:Humans; D007564:Japan / Q000453:epidemiology; D008297:Male; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D012336:RNA, Ribosomal, 16S
keywords: Capnocytophaga canimorsus; Distribution; Phylogenetic analysis; Pulsed-field gel electrophoresis (PFGE); Zoonosis