title: Estrogen improves the proliferation and differentiation of hBMSCs derived from postmenopausal osteoporosis through notch signaling pathway.
abstract: Estrogen deficiency is the main reason of bone loss, leading to postmenopausal osteoporosis, and estrogen replacement therapy (ERT) has been demonstrated to protect bone loss efficiently. Notch signaling controls proliferation and differentiation of bone marrow-derived mesenchymal stem cells (BMSCs). Moreover, imperfect estrogen-responsive elements (EREs) were found in the 5'-untranslated region of Notch1 and Jagged1. Thus, we examined the molecular and biological links between estrogen and the Notch signaling in postmenopausal osteoporosis in vitro. hBMSCs were obtained from healthy women and patients with postmenopausal osteoporosis. Notch signaling molecules were quantified using real-time polymerase chain reaction (real-time PCR) and Western Blot. Luciferase reporter constructs with putative EREs were transfected into hBMSCs and analyzed. hBMSCs were transduced with lentiviral vectors containing human Notch1 intracellular domain (NICD1). We also used N-[N-(3, 5-diflurophenylacetate)-l-alanyl]-(S)-phenylglycine t-butyl ester, a γ-secretase inhibitor, to suppress the Notch signaling. We found that estrogen enhanced the Notch signaling in hBMSCs by promoting the expression of Jagged1. hBMSCs cultured with estrogen resulted in the up-regulation of Notch signaling and increased proliferation and differentiation. Enhanced Notch signaling could enhance the proliferation and differentiation of hBMSCs from patients with postmenopausal osteoporosis (OP-hBMSCs). Our results demonstrated that estrogen preserved bone mass partly by activating the Notch signaling. Because long-term ERT has been associated with several side effects, the Notch signaling could be a potential target for treating postmenopausal osteoporosis.
authors.affiliation: Institute of Orthopedic Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, 710032, People's Republic of China.
authors.forename: Jin-Zhu
authors.initials: JZ
authors.lastname: Fan
authors.forename: Liu
authors.initials: L
authors.lastname: Yang
authors.forename: Guo-Lin
authors.initials: GL
authors.lastname: Meng
authors.forename: Yan-shui
authors.initials: YS
authors.lastname: Lin
authors.forename: Bo-Yuan
authors.initials: BY
authors.lastname: Wei
authors.forename: Jing
authors.initials: J
authors.lastname: Fan
authors.forename: Hui-Min
authors.initials: HM
authors.lastname: Hu
authors.forename: Yan-Wu
authors.initials: YW
authors.lastname: Liu
authors.forename: Shi
authors.initials: S
authors.lastname: Chen
authors.forename: Jin-Kang
authors.initials: JK
authors.lastname: Zhang
authors.forename: Qi-Zhen
authors.initials: QZ
authors.lastname: He
authors.forename: Zhuo-Jing
authors.initials: ZJ
authors.lastname: Luo
authors.forename: Jian
authors.initials: J
authors.lastname: Liu
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D004967:Estrogens / Q000494:pharmacology*; D005260:Female; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects*; D008875:Middle Aged; D015663:Osteoporosis, Postmenopausal / Q000473:pathology*; D051880:Receptors, Notch / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D004967:Estrogens; D051880:Receptors, Notch
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