title: Baicalin inhibits TLR7/MYD88 signaling pathway activation to suppress lung inflammation in mice infected with influenza A virus.
abstract: The present study aimed to investigate the protective effects and underlying mechanisms of baicalin on imprinting control region mice infected with influenza A/FM/1/47 (H1N1) virus. Oral administration of baicalin into mice infected with H1N1 prevented death, increased the mean time to death and inhibited lung index and lung consolidation. Subsequently, fluorescence quantitative polymerase chain reaction was used to assess the mRNA expression of toll-like receptor 7 (TLR7) and myeloid differentiation primary response gene 88 (MYD88), and western blot analysis was used to determine the expression of phosphorylated nuclear factor κB (NF-κB)-P65 and c-jun/activator protein 1 (AP-1). An enzyme-linked immunosorbent assay was applied to test for the inflammatory cytokines, tumor necrosis factor (TNF)-α and interleukin (IL)-1β and IL-6, in the lung tissue. The findings indicated that baicalin downregulated the mRNA expression of TLR7 and MYD88, significantly downregulated the protein expression of NF-κB-P65 and AP-1 and also inhibited the secretion of TNF-α, IL-1β and IL-6. In conclusion, baicalin effectively reduced the pathological damage and inflammation of the lungs by downregulating the TLR7/MYD88-mediated signaling pathway.
authors.affiliation: Department of Pathogenic Biology and Immunology, Basic Medical Science College, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China.
authors.forename: Qiaofeng
authors.initials: Q
authors.lastname: Wan
authors.affiliation: Department of Pathogenic Biology and Immunology, Basic Medical Science College, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China.
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Pathogenic Biology and Immunology, Basic Medical Science College, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China.
authors.forename: Xuebo
authors.initials: X
authors.lastname: Han
authors.affiliation: Department of Pathogenic Biology and Immunology, Basic Medical Science College, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Lin
authors.affiliation: Department of Pathogenic Biology and Immunology, Basic Medical Science College, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China.
authors.forename: Yanhui
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Key Laboratory of Antivirus of the Ministry of Education, Beijing University of Chinese Medicine, Beijing 100029, P.R. China.
authors.forename: Ligang
authors.initials: L
authors.lastname: Gu
authors.affiliation: Department of Pathogenic Biology and Immunology, Basic Medical Science College, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China.
authors.forename: Jiaqing
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of Pathogenic Biology and Immunology, Basic Medical Science College, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Pathogenic Biology and Immunology, Basic Medical Science College, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China.
authors.forename: Ling
authors.initials: L
authors.lastname: Huang
authors.affiliation: Department of Pathogenic Biology and Immunology, Basic Medical Science College, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China.
authors.forename: Yanbin
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Pathogenic Biology and Immunology, Basic Medical Science College, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China.
authors.forename: Yurong
authors.initials: Y
authors.lastname: Yang
keywords: TLR7/MYD88; baicalin; c-jun/activator protein 1; influenza A virus; lung; nuclear factor κB
references.citation: Int Arch Allergy Immunol. 1997 May-Jul;113(1-3):307-11
references.pmid: 9130560
references.citation: Proc Natl Acad Sci U S A. 2009 Mar 31;106(13):5306-11
references.pmid: 19279209
references.citation: Ann N Y Acad Sci. 2008 Nov;1143:1-20
references.pmid: 19076341
references.citation: J Biol Chem. 2001 Jun 15;276(24):10990-8
references.pmid: 11441823
references.citation: J Med Virol. 2007 Oct;79(10):1569-76
references.pmid: 17705168
references.citation: Indian J Exp Biol. 2012 Sep;50(9):612-7
references.pmid: 23140018
references.citation: Science. 2007 Mar 2;315(5816):1274-8
references.pmid: 17332413
references.citation: Cell Host Microbe. 2012 Jun 14;11(6):643-53
references.pmid: 22704624
references.citation: Ann N Y Acad Sci. 2013 Apr;1283:67-74
references.pmid: 23163321
references.citation: Science. 1995 Sep 15;269(5230):1583-5
references.pmid: 7667639
references.citation: Antiviral Res. 2005 Dec;68(3):124-34
references.pmid: 16188329
references.citation: J Virol. 2008 Oct;82(20):9880-9
references.pmid: 18701591
references.citation: Sci STKE. 2007 Sep 04;2007(402):pe48
references.pmid: 17785715
references.citation: Biol Pharm Bull. 2010;33(2):238-43
references.pmid: 20118546
references.citation: Antiviral Res. 2005 Nov;68(2):75-83
references.pmid: 16122816
references.citation: Adv Exp Med Biol. 2013;756:271-83
references.pmid: 22836645
references.citation: Zhongguo Zhong Yao Za Zhi. 2007 Nov;32(22):2413-5
references.pmid: 18257272
references.citation: Methods. 2001 Dec;25(4):402-8
references.pmid: 11846609
references.citation: J Gen Virol. 2004 Aug;85(Pt 8):2347-2356
references.pmid: 15269376
references.citation: Mol Cell Biochem. 2002 May-Jun;234-235(1-2):239-48
references.pmid: 12162440
references.citation: Nature. 2005 Oct 20;437(7062):1108
references.pmid: 16228009
references.citation: Curr Top Microbiol Immunol. 2007;316:167-92
references.pmid: 17969448
references.citation: Antiviral Res. 1998 Feb;37(2):131-40
references.pmid: 9588845
references.citation: Biomed Rep. 2013 Sep;1(5):766-770
references.pmid: 24649026