title: Prevalence and distribution of Aggregatibacter actinomycetemcomitans and its cdtB gene in subgingival plaque of Chinese periodontitis patients.
abstract: BACKGROUND
  Aggregatibacter actinomycetemcomitans (A.actinomycetemcomitans) is an important periodontal pathogen that can participate in periodontitis and other non-oral infections. The cytolethal distending toxin (Cdt) is among the virulence factors produced by this bacterium. This study was to elucidate the distribution of A.actinomycetemcomitans and the prevalence of its cdtB gene in Chinese subjects.
  
  
  METHODS
  A total of 255 subgingival samples were obtained from 30 subjects. Samples were collected from periodontal healthy sites as well as shallow, moderate and deep pockets. The absolute quantity of A.actinomycetemcomitans and cdtB gene were determined by real-time polymerase chain reaction.
  
  
  RESULTS
  A.actinomycetemcomitans was detected in 92 of 105 (87.6%) samples of aggressive periodontitis (AgP) patients, in 73 of 79 (92.4%) samples of chronic periodontitis ( CP) patients and in 5 of 71 (7.0%) samples of periodontal healthy subjects. The cdtB gene was detected in 72 sites (78.3%) with AgP infected with A.actinomycetemcomitans, 54 sites (74.0%) with CP infected with A.actinomycetemcomitans and none in healthy sites infected with A.actinomycetemcomitans. In addition, quantity of A.actinomycetemcomitans and cdt gene in samples from deep pockets were significant larger than moderate, shallow and healthy sites (P < 0.05). In comparison to CP, AgP patients were infected with increased numbers of cdt genotype in deep pockets (P < 0.05).
  
  
  CONCLUSIONS
  This study suggests that the cdtB gene are prevalent in A.actinomycetemcomitans, and the distribution of cdt genotype strain may be correlated with AgP and serious periodontal inflammation.
authors.forename: Xiaoqian
authors.initials: X
authors.lastname: Wang
authors.forename: Lu
authors.initials: L
authors.lastname: Li
authors.forename: Mifang
authors.initials: M
authors.lastname: Yang
authors.forename: Ying
authors.initials: Y
authors.lastname: Geng
authors.forename: Huiping
authors.initials: H
authors.lastname: Chen
authors.affiliation: Laboratory of Oral Infection and Immunology, Institute of Stomatology, Nanjing Medical University, Nanjing, Jiang Su, China. yanxu@njmu.edu.cn.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xu
authors.forename: Ying
authors.initials: Y
authors.lastname: Sun
mesh_terms: D000328:Adult; D016976:Aggregatibacter actinomycetemcomitans / Q000235:genetics / Q000302:isolation & purification*; D010520:Aggressive Periodontitis / Q000382:microbiology; D058491:Bacterial Load; D001427:Bacterial Toxins / Q000032:analysis* / Q000235:genetics; D002681:China / Q000208:ethnology; D055113:Chronic Periodontitis / Q000382:microbiology; D004269:DNA, Bacterial / Q000032:analysis; D003773:Dental Plaque / Q000382:microbiology*; D005006:Ethnicity / Q000235:genetics; D005260:Female; D014644:Genetic Variation / Q000235:genetics; D005838:Genotype; D006801:Humans; D008297:Male; D017622:Periodontal Attachment Loss / Q000382:microbiology; D010514:Periodontal Pocket / Q000382:microbiology; D010518:Periodontitis / Q000382:microbiology*; D010519:Periodontium / Q000382:microbiology; D021122:Protein Subunits / Q000032:analysis* / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D037521:Virulence Factors / Q000032:analysis / Q000235:genetics
chemical_list: D001427:Bacterial Toxins; D004269:DNA, Bacterial; D021122:Protein Subunits; D037521:Virulence Factors; C447141:cytolethal distending toxin, Actinobacillus actinomycetemcomitans
references.citation: J Med Microbiol. 2000 Oct;49(10):861-74
references.pmid: 11023183
references.citation: Quintessence Int. 2009 Jan;40(1):53-60
references.pmid: 19159024
references.citation: Infect Immun. 2004 Feb;72(2):1116-25
references.pmid: 14742559
references.citation: J Clin Microbiol. 2009 Jul;47(7):2018-25
references.pmid: 19458180
references.citation: J Clin Microbiol. 2001 Oct;39(10):3637-48
references.pmid: 11574585
references.citation: Oral Microbiol Immunol. 2002 Aug;17(4):231-8
references.pmid: 12121473
references.citation: J Clin Periodontol. 2007 Jul;34(7):566-73
references.pmid: 17433043
references.citation: Oral Microbiol Immunol. 2009 Dec;24(6):493-501
references.pmid: 19832802
references.citation: J Periodontol. 2000 May;71(5 Suppl):867-9
references.pmid: 10875695
references.citation: J Periodontal Res. 2010 Oct;45(5):635-42
references.pmid: 20546109
references.citation: Mol Microbiol. 2000 Aug;37(4):952-63
references.pmid: 10972814
references.citation: Microb Pathog. 2007 May-Jun;42(5-6):174-83
references.pmid: 17353111
references.citation: Infect Immun. 1998 Oct;66(10):5008-19
references.pmid: 9746611
references.citation: Science. 2000 Oct 13;290(5490):354-7
references.pmid: 11030657
references.citation: J Clin Microbiol. 2002 Apr;40(4):1181-7
references.pmid: 11923328
references.citation: Braz Oral Res. 2010 Apr-Jun;24(2):217-23
references.pmid: 20658042
references.citation: J Periodontol. 2007 Jan;78(1):72-8
references.pmid: 17199542
references.citation: J Clin Microbiol. 2003 Feb;41(2):863-6
references.pmid: 12574302
references.citation: Proc Natl Acad Sci U S A. 2004 Mar 30;101(13):4614-9
references.pmid: 15070766
references.citation: J Periodontol. 2007 May;78(5):909-17
references.pmid: 17470026
references.citation: J Periodontal Res. 2002 Aug;37(4):268-72
references.pmid: 12200970
references.citation: J Med Microbiol. 2001 Oct;50(10):860-864
references.pmid: 11599734
references.citation: J Clin Microbiol. 2003 Apr;41(4):1391-8
references.pmid: 12682119
references.citation: J Microbiol Methods. 2004 Oct;59(1):117-25
references.pmid: 15325758
references.citation: Ann Periodontol. 1999 Dec;4(1):1-6
references.pmid: 10863370
references.citation: J Periodontal Res. 2003 Oct;38(5):518-24
references.pmid: 12941077