title: Dual-polarization Raman spectral imaging to extract overlapping molecular fingerprints of living cells.
abstract: Raman spectral imaging is gaining more and more attention in biological studies because of its label-free characteristic. However, the discrimination of overlapping chemical contrasts has been a major challenge. In this study, we introduce an optical method to simultaneously obtain two orthogonally polarized Raman images from a single scan of the sample. We demonstrate how this technique can improve the quality and quantity of the hyperspectral Raman dataset and how the technique is expected to further extend the horizons of Raman spectral imaging in biological studies by providing more detailed chemical information. The dual-polarization Raman images of a HeLa cell.
authors.affiliation: Department of Applied Physics, Osaka University, 2-1 Yamadaoka, Osaka 565-0871, Japan.
authors.forename: Liang-da
authors.initials: LD
authors.lastname: Chiu
authors.affiliation: Department of Applied Physics, Osaka University, 2-1 Yamadaoka, Osaka 565-0871, Japan.|Sodeoka Live Cell Chemistry Project, ERATO, Japan Sciences and Technology Agency, 2-1 Hirosawa, Saitama 351-0198, Japan.
authors.forename: Almar F
authors.initials: AF
authors.lastname: Palonpon
authors.affiliation: Immunology Frontier Research Center, Osaka University, 2-1 Yamada-ka, Suita, Osaka 565-0871, Japan.
authors.forename: Nicholas I
authors.initials: NI
authors.lastname: Smith
authors.affiliation: Department of Applied Physics, Osaka University, 2-1 Yamadaoka, Osaka 565-0871, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kawata
authors.affiliation: Sodeoka Live Cell Chemistry Project, ERATO, Japan Sciences and Technology Agency, 2-1 Hirosawa, Saitama 351-0198, Japan.|Synthetic Organic Chemistry Laboratory, RIKEN, 2-1 Hirosawa, Saitama 351-0198, Japan.
authors.forename: Mikiko
authors.initials: M
authors.lastname: Sodeoka
authors.affiliation: Department of Applied Physics, Osaka University, 2-1 Yamadaoka, Osaka 565-0871, Japan. fujita@ap.eng.osaka-u.ac.jp.
authors.forename: Katsumasa
authors.initials: K
authors.lastname: Fujita
mesh_terms: D002470:Cell Survival; D045304:Cytochromes c / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008853:Microscopy / Q000379:methods*; D057054:Molecular Imaging / Q000379:methods*; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D045304:Cytochromes c
keywords: Raman imaging; background removal; bio-imaging; polarized Raman