title: Endothelin-1 activation of the endothelin B receptor modulates pulmonary endothelial CX3CL1 and contributes to pulmonary angiogenesis in experimental hepatopulmonary syndrome.
abstract: Hepatic production and release of endothelin-1 (ET-1) binding to endothelin B (ETB) receptors, overexpressed in the lung microvasculature, is associated with accumulation of pro-angiogenic monocytes and vascular remodeling in experimental hepatopulmonary syndrome (HPS) after common bile duct ligation (CBDL). We have recently found that lung vascular monocyte adhesion and angiogenesis in HPS involve interaction of endothelial C-X3-C motif ligand 1 (CX3CL1) with monocyte CX3C chemokine receptor 1 (CX3CR1), although whether ET-1/ETB receptor activation influences these events is unknown. Our aim was to define if ET-1/ETB receptor activation modulates CX3CL1/CX3CR1 signaling and lung angiogenesis in experimental HPS. A selective ETB receptor antagonist, BQ788, was given for 2 weeks to 1-week CBDL rats. ET-1 (±BQ788) was given to cultured rat pulmonary microvascular endothelial cells overexpressing ETB receptors. BQ788 treatment significantly decreased lung angiogenesis, monocyte accumulation, and CX3CL1 levels after CBDL. ET-1 treatment significantly induced CX3CL1 production in lung microvascular endothelial cells, which was blocked by inhibitors of Ca(2+) and mitogen-activated protein kinase (MEK)/ERK pathways. ET-1-induced ERK activation was Ca(2+) independent. ET-1 administration also increased endothelial tube formation in vitro, which was inhibited by BQ788 or by blocking Ca(2+) and MEK/ERK activation. CX3CR1 neutralizing antibody partially inhibited ET-1 effects on tube formation. These findings identify a novel mechanistic interaction between the ET-1/ETB receptor axis and CX3CL1/CX3CR1 in mediating pulmonary angiogenesis and vascular monocyte accumulation in experimental HPS.
authors.affiliation: Division of Gastroenterology, Hepatology, and Nutrition, the Department of Internal Medicine, The University of Texas Health Science Center at Houston, Houston, Texas.
authors.forename: Junlan
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Division of Gastroenterology, Hepatology, and Nutrition, the Department of Internal Medicine, The University of Texas Health Science Center at Houston, Houston, Texas.
authors.forename: Wenli
authors.initials: W
authors.lastname: Yang
authors.affiliation: Division of Gastroenterology, Hepatology, and Nutrition, the Department of Internal Medicine, The University of Texas Health Science Center at Houston, Houston, Texas.
authors.forename: Bingqian
authors.initials: B
authors.lastname: Hu
authors.affiliation: Division of Gastroenterology, Hepatology, and Nutrition, the Department of Internal Medicine, The University of Texas Health Science Center at Houston, Houston, Texas.
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
authors.affiliation: Division of Gastroenterology, Hepatology, and Nutrition, the Department of Internal Medicine, The University of Texas Health Science Center at Houston, Houston, Texas. Electronic address: michael.b.fallon@uth.tmc.edu.
authors.forename: Michael B
authors.initials: MB
authors.lastname: Fallon
mesh_terms: D000818:Animals; D020013:Calcium Signaling; D002478:Cells, Cultured; D054428:Chemokine CX3CL1 / Q000378:metabolism*; D042783:Endothelial Cells / Q000378:metabolism* / Q000473:pathology; D019332:Endothelin-1 / Q000378:metabolism*; D020065:Hepatopulmonary Syndrome / Q000378:metabolism* / Q000473:pathology; D008168:Lung / Q000098:blood supply / Q000378:metabolism* / Q000473:pathology; D020935:MAP Kinase Signaling System; D008297:Male; D009389:Neovascularization, Pathologic / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D044023:Receptor, Endothelin B / Q000378:metabolism*
chemical_list: D054428:Chemokine CX3CL1; C515540:Cx3cl1 protein, rat; D019332:Endothelin-1; D044023:Receptor, Endothelin B
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