title: Functional analysis reveals angiogenic potential of human mesenchymal stem cells from Wharton's jelly in dermal regeneration.
abstract: Disorders in skin wound healing are a major health problem that requires the development of innovative treatments. The use of biomaterials as an alternative of skin replacement has become relevant, but its use is still limited due to poor vascularization inside the scaffolds, resulting in insufficient oxygen and growth factors at the wound site. In this study, we have developed a cell-based wound therapy consisting of the application of collagen-based dermal scaffolds containing mesenchymal stem cells from Wharton's jelly (WJ-MSC) in an immunocompetent mouse model of angiogenesis. From our comparative study on the secretion profile between WJ-MSC and adipose tissue-derived MSC, we found a stronger expression of several well-characterized growth factors, such as VEGF-A, angiopoietin-1 and aFGF, which are directly linked to angiogenesis, in the culture supernatant of WJ-MSC, both on monolayer and 3D culture conditions. WJ-MSC proved to be angiogenic both in vitro and in vivo, through tubule formation and CAM assays, respectively. Moreover, WJ-MSC consistently improved the healing response in vivo in a mouse model of human-like dermal repair, by triggering angiogenesis and further providing a suitable matrix for wound repair, without altering the inflammatory response in the animals. Since these cells can be easily isolated, cultured with high expansion rates and cryopreserved, they represent an attractive stem cell source for their use in allogeneic cell transplant and tissue engineering.
authors.affiliation: FONDAP Center for Genome Regulation, Laboratory of Stem Cells and Development, Faculty of Sciences, Universidad de Chile, Santiago, Chile.
authors.forename: Sandra S
authors.initials: SS
authors.lastname: Edwards
authors.forename: Gabriela
authors.initials: G
authors.lastname: Zavala
authors.forename: Catalina P
authors.initials: CP
authors.lastname: Prieto
authors.forename: Matías
authors.initials: M
authors.lastname: Elliott
authors.forename: Samuel
authors.initials: S
authors.lastname: Martínez
authors.forename: Jose T
authors.initials: JT
authors.lastname: Egaña
authors.forename: María R
authors.initials: MR
authors.lastname: Bono
authors.forename: Verónica
authors.initials: V
authors.lastname: Palma
mesh_terms: D017667:Adipocytes / Q000166:cytology; D000818:Animals; D001672:Biocompatible Materials; D049109:Cell Proliferation; D002645:Chickens; D049033:Chorioallantoic Membrane; D015925:Cryopreservation; D017077:Culture Media, Conditioned; D005434:Flow Cytometry; D006801:Humans; D007249:Inflammation; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009389:Neovascularization, Pathologic; D010012:Osteogenesis; D020543:Proteome; D012038:Regeneration / Q000502:physiology*; D012867:Skin / Q000378:metabolism* / Q000473:pathology; D023822:Tissue Engineering; D014470:Umbilical Cord / Q000473:pathology; D059631:Wharton Jelly / Q000737:chemistry*; D014945:Wound Healing
chemical_list: D001672:Biocompatible Materials; D017077:Culture Media, Conditioned; D020543:Proteome