title: Synthesis, characterization, and evaluation of antibacterial effect of Ag nanoparticles against Escherichia coli O157:H7 and methicillin-resistant Staphylococcus aureus (MRSA).
abstract: Silver nanoparticles (AgNPs) have been shown great interest because of their potential antibacterial effect. Recently, this has been increased due to resistance in some pathogenic bacteria strains to conventional antibiotics, which has initiated new studies to search for more effective treatments against resistant microorganisms. For these reasons, AgNPs have become an important approach for applications in nanobiotechnology in the development of antibiotic treatment of different bacterial infections. This study was aimed at synthesizing AgNPs using cysteine as a reducer agent and cetyl-tri-methyl-ammonium bromide as a stabilizer in order to obtain more efficient treatment against the pathogen bacteria Escherichia coli O157:H7. These AgNPs were characterized through UV-Vis spectroscopy, transmission electron microscopy, and dynamic light scattering. From these analyses, formation of spherical nanoparticles with an average size of 55 nm was confirmed. Finally, minimal inhibitory concentration (MIC) and minimal bactericide concentration (MBC) of these AgNPs against pathogenic strains E. coli O157:H7 and methicillin-resistant Staphylococcus aureus (MRSA) were determined in both solid and liquid media. MIC and MBC values were around 0.25 μg/mL and 1 μg/mL, respectively. These parameters were comparable to those reported in the literature and were even more effective than other synthesized AgNPs.
authors.affiliation: Escuela de Química, Facultad de Ciencias, Universidad Industrial de Santander, Colombia.
authors.forename: Daissy
authors.initials: D
authors.lastname: Paredes
authors.affiliation: Escuela de Bacteriología y Laboratorio Clínico, Facultad de Salud, Universidad Industrial de Santander, Colombia.
authors.forename: Claudia
authors.initials: C
authors.lastname: Ortiz
authors.affiliation: Escuela de Química, Facultad de Ciencias, Universidad Industrial de Santander, Colombia.
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Torres
mesh_terms: D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D019453:Escherichia coli O157 / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D053768:Metal Nanoparticles / Q000008:administration & dosage* / Q000648:ultrastructure; D055624:Methicillin-Resistant Staphylococcus aureus / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D010316:Particle Size; D012834:Silver / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D012834:Silver
keywords: MBC; MIC; antibacterial activity; minimal bactericide concentration; minimal inhibitory concentration; nanomaterials
references.citation: Langmuir. 2005 Oct 11;21(21):9651-9
references.pmid: 16207049
references.citation: Environ Sci Technol. 2006 Dec 1;40(23):7141-9
references.pmid: 17180960
references.citation: Chin Med J (Engl). 2002 Jun;115(6):815-8
references.pmid: 12123543
references.citation: Acta Biomater. 2008 May;4(3):707-16
references.pmid: 18248860
references.citation: Infect Control Hosp Epidemiol. 2003 May;24(5):362-86
references.pmid: 12785411
references.citation: Annu Rev Phys Chem. 2003;54:331-66
references.pmid: 12626731
references.citation: Nanomedicine. 2007 Mar;3(1):95-101
references.pmid: 17379174
references.citation: Nanomedicine. 2010 Oct;6(5):681-8
references.pmid: 20215045
references.citation: Nanomedicine (Lond). 2009 Aug;4(6):637-43
references.pmid: 19663592
references.citation: Nanotechnology. 2005 Oct;16(10):2346-53
references.pmid: 20818017
references.citation: Bacteriol Rev. 1972 Dec;36(4):407-77
references.pmid: 4568761
references.citation: Toxicol Lett. 2008 Jan 4;176(1):1-12
references.pmid: 18022772
references.citation: J Biosci Bioeng. 2006 Jul;102(1):1-7
references.pmid: 16952829
references.citation: J Biol Inorg Chem. 2007 May;12(4):527-34
references.pmid: 17353996
references.citation: Adv Colloid Interface Sci. 2004 Jun 30;110(1-2):49-74
references.pmid: 15142823
references.citation: Salud Publica Mex. 2005 Sep-Oct;47(5):381-7
references.pmid: 16323532
references.citation: Colloids Surf B Biointerfaces. 2010 Mar 1;76(1):164-9
references.pmid: 19954933
references.citation: Clin Microbiol Rev. 1998 Jan;11(1):142-201
references.pmid: 9457432
references.citation: Microbiol Mol Biol Rev. 2003 Dec;67(4):593-656
references.pmid: 14665678
references.citation: Biochim Biophys Acta. 2004 Jan 14;1696(1):1-14
references.pmid: 14726199
references.citation: Lancet. 1993 Apr 10;341(8850):961
references.pmid: 8096294
references.citation: Nanomedicine. 2009 Dec;5(4):452-6
references.pmid: 19523420
references.citation: Nanomedicine. 2011 Jun;7(3):305-14
references.pmid: 21094275
references.citation: Biotechnol Adv. 2009 Jan-Feb;27(1):76-83
references.pmid: 18854209
references.citation: Environ Sci Technol. 2009 Mar 1;43(5):1425-9
references.pmid: 19350914
references.citation: Antimicrob Agents Chemother. 1996 Sep;40(9):2099-105
references.pmid: 8878588
references.citation: Anal Chem. 2005 Aug 15;77(16):5258-67
references.pmid: 16097767
references.citation: J Phys Chem B. 2006 Aug 24;110(33):16248-53
references.pmid: 16913750
references.citation: J Colloid Interface Sci. 2004 Nov 15;279(2):458-63
references.pmid: 15464811
references.citation: J Biol Chem. 1959 Dec;234:3263-8
references.pmid: 13835291
references.citation: Rev Clin Esp. 1997 Sep;197 Suppl 2:74-9
references.pmid: 9441327
references.citation: J Colloid Interface Sci. 2004 Jul 1;275(1):177-82
references.pmid: 15158396
references.citation: J Microbiol Methods. 2003 Nov;55(2):441-6
references.pmid: 14529966