title: TGF-beta signaling-dependent alleviation of dextran sulfate sodium-induced colitis by mesenchymal stem cell transplantation.
abstract: Alleviation of dextran sulfate sodium (DSS)-induced colitis was shown after by transplantation of bone marrow derived cells in mice. Nevertheless, the underlying mechanism remains elusive. In the present study, we transplanted primary mouse mesenchymal stem cells (MSC) into isogeneic mice with DSS-induced colitis. We found that MSC transplantation significantly alleviated the DSS-induced colitis. Inhibition of transforming growth factor beta (TGF-beta) signaling abrogated the therapeutic effect of MSC transplantation on DSS-colitis, suggesting a TGF-beta signaling-dependent manner. Moreover, MSC transplantation seemed to induce M2 macrophage polarization, which appeared to be the major source of TGF-beta in this model. Our data thus demonstrate that MSC transplantation may activate TGF-beta signaling pathways to promote the recovery of DSS-colitis.
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Zhengzhou University, 1 Construction East Road, Zhengzhou, 450052, Henan Province, China.
authors.forename: Chong
authors.initials: C
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.forename: Ling
authors.initials: L
authors.lastname: Sun
authors.forename: Yanfang
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000818:Animals; D015153:Blotting, Western; D003092:Colitis / Q000139:chemically induced* / Q000628:therapy*; D016264:Dextran Sulfate / Q000009:adverse effects*; D005434:Flow Cytometry; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D051379:Mice; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000502:physiology*; D016212:Transforming Growth Factor beta / Q000378:metabolism*
chemical_list: D016212:Transforming Growth Factor beta; D016264:Dextran Sulfate
references.citation: Clin Exp Immunol. 1998 Dec;114(3):385-91
references.pmid: 9844047
references.citation: Cell. 2001 May 4;105(3):369-77
references.pmid: 11348593
references.citation: Pharmacology. 2007;80(4):193-9
references.pmid: 17587885
references.citation: Nat Protoc. 2007;2(3):541-6
references.pmid: 17406617
references.citation: Clin Exp Immunol. 2003 Oct;134(1):120-6
references.pmid: 12974764
references.citation: Science. 2002 Sep 27;297(5590):2256-9
references.pmid: 12215650
references.citation: Life Sci. 1993;53(25):1867-72
references.pmid: 8255146
references.citation: Mol Pharmacol. 2002 Jul;62(1):58-64
references.pmid: 12065755
references.citation: J Histochem Cytochem. 2003 Sep;51(9):1177-89
references.pmid: 12923243
references.citation: Nature. 2003 Apr 24;422(6934):901-4
references.pmid: 12665833
references.citation: Nature. 2002 Apr 4;416(6880):485-7
references.pmid: 11932725
references.citation: Hematology. 2005;10 Suppl 1:293-6
references.pmid: 16188690
references.citation: Nature. 2002 Jul 4;418(6893):41-9
references.pmid: 12077603
references.citation: J Med Chem. 2002 Feb 28;45(5):999-1001
references.pmid: 11855979
references.citation: Life Sci. 2008 Dec 5;83(23-24):771-9
references.pmid: 18950645
references.citation: Acta Pharmacol Sin. 2007 Jan;28(1):89-97
references.pmid: 17184587
references.citation: J Exp Med. 1996 Jun 1;183(6):2605-16
references.pmid: 8676081
references.citation: Methods Mol Biol. 2010;633:197-206
references.pmid: 20204629
references.citation: Gastroenterology. 1990 Mar;98(3):694-702
references.pmid: 1688816
references.citation: Hepatogastroenterology. 1990 Dec;37 Suppl 2:110-2
references.pmid: 2083921
references.citation: Inflamm Bowel Dis. 2011 Sep;17(9):1925-35
references.pmid: 21830271
references.citation: Nature. 2002 Apr 4;416(6880):542-5
references.pmid: 11932747
references.citation: Am J Gastroenterol. 1992 Aug;87(8):971-6
references.pmid: 1642220
references.citation: J Gastroenterol. 2011 Feb;46(2):143-52
references.pmid: 20848145
references.citation: Exp Cell Res. 2004 May 1;295(2):395-406
references.pmid: 15093739
references.citation: Mol Pharmacol. 2002 Jul;62(1):65-74
references.pmid: 12065756
references.citation: Inflamm Bowel Dis. 2004 Sep;10(5):632-6
references.pmid: 15472526
references.citation: Gut. 2006 May;55(5):604-6
references.pmid: 16609133
references.citation: Nature. 2003 Apr 24;422(6934):897-901
references.pmid: 12665832