title: Iodine penetration and glycogen distribution in vital staining of oral mucosa with iodine solution.
abstract: OBJECTIVE
  To assess and compare iodine penetration and glycogen distribution in a vital staining of oral mucosa with iodine solution.
  
  
  METHODS
  Twenty samples were obtained including both iodine-stained and -unstained mucosa. Intraepithelial iodine was examined using frozen sections. Glycogen distribution was assessed by periodic acid-Schiff staining and transmission electron microscopy.
  
  
  RESULTS
  Iodine accumulation was observed mainly superficially and in the upper and middle thirds of prickle cell layers, with glycogen in almost the whole epithelium except for the para- and basal cell layers. The pattern of iodine and glycogen distribution was classified into 3 types (full-, surround-, and scatter-type). The iodine color was mainly derived from the cells with full- and surround-type distributed glycogen in the upper half of the oral epithelium.
  
  
  CONCLUSIONS
  The results of this study suggested that iodine penetrated into nonkeratinized oral epithelium and reacted mainly with intraepithelial glycogen homogeneously distributed in the cytoplasm.
authors.affiliation: Department of Dentistry and Oral Surgery, Shinshu University School of Medicine, Matsumoto, Japan; Department of Orthodontics, Second Hospital of Hebei Medical University, Shijiazhuang, China.
authors.forename: Tiepeng
authors.initials: T
authors.lastname: Xiao
authors.affiliation: Department of Dentistry and Oral Surgery, Shinshu University School of Medicine, Matsumoto, Japan. Electronic address: hkurita@shinshu-u.ac.jp.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kurita
authors.affiliation: Department of Dentistry and Oral Surgery, Shinshu University School of Medicine, Matsumoto, Japan; Department of Oral and Maxillofacial Surgery, Stomatology Hospital of Hebei Medical University, Shijiazhuang, China.
authors.forename: Xiangjun
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Dentistry and Oral Surgery, Shinshu University School of Medicine, Matsumoto, Japan.
authors.forename: Fangfang
authors.initials: F
authors.lastname: Qi
authors.affiliation: Department of Dentistry and Oral Surgery, Shinshu University School of Medicine, Matsumoto, Japan.
authors.forename: Tetsu
authors.initials: T
authors.lastname: Shimane
authors.affiliation: Department of Dentistry and Oral Surgery, Shinshu University School of Medicine, Matsumoto, Japan.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Aizawa
authors.affiliation: Department of Dentistry and Oral Surgery, Shinshu University School of Medicine, Matsumoto, Japan.
authors.forename: Shinobu
authors.initials: S
authors.lastname: Uehara
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D005629:Frozen Sections; D006003:Glycogen / Q000378:metabolism*; D006801:Humans; D007455:Iodine / Q000493:pharmacokinetics*; D008297:Male; D008854:Microscopy, Electron; D008875:Middle Aged; D009061:Mouth Mucosa / Q000378:metabolism* / Q000648:ultrastructure; D013194:Staining and Labeling
chemical_list: D006003:Glycogen; D007455:Iodine