title: The action of JAK, SMAD and ERK signal pathways on hepcidin suppression by polysaccharides from Angelica sinensis in rats with iron deficiency anemia.
abstract: A crude polysaccharide was obtained by water extraction and ethanol precipitation from the root of Angelica sinensis (AS) to investigate its suppressive effect on hepcidin expression in rats with iron deficiency anemia (IDA). In this study, we showed that the Angelica sinensis polysaccharide (ASP) was mainly composed of arabinose, glucose and galactose in a molar ratio of 1 : 2.5 : 7.5, with the molecular weight of 72 900 Da and the sugar content of 95.1%. ASP markedly decreased hepcidin expression by suppressing the expression of JAK1/2, phospho-JAK1/2, phospho-SMAD1/5/8, phospho-ERK1/2, and promoting the expression of SMAD7 in the liver. These findings suggest that ASP can prevent the janus-kinase (JAK), son of mother against decapentaplegic (SMAD) and extracellular signal-regulated kinase (ERK) pathways to down-regulate hepcidin expression in IDA rats, and may be useful for the treatments of diseases induced by hepcidin over-expression.
authors.affiliation: Union Hospital of Huazhong University of Science and Technology, Department of Pharmacy, No. 1227, Jiefang Road, 430030 Wuhan, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yao
authors.initials: Y
authors.lastname: Cheng
authors.forename: Na
authors.initials: N
authors.lastname: Wang
authors.forename: Qilin
authors.initials: Q
authors.lastname: Zhang
authors.forename: Kaiping
authors.initials: K
authors.lastname: Wang
mesh_terms: D018798:Anemia, Iron-Deficiency / Q000188:drug therapy*; D029971:Angelica sinensis / Q000737:chemistry*; D000818:Animals; D015536:Down-Regulation; D048049:Extracellular Signal-Regulated MAP Kinases / Q000235:genetics / Q000378:metabolism; D064451:Hepcidins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D019266:Iron, Dietary / Q000008:administration & dosage / Q000097:blood; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism; D008970:Molecular Weight; D018517:Plant Roots / Q000737:chemistry; D011134:Polysaccharides / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction; D051898:Smad1 Protein / Q000235:genetics / Q000378:metabolism; D051902:Smad5 Protein / Q000235:genetics / Q000378:metabolism; D051906:Smad7 Protein / Q000235:genetics / Q000378:metabolism; D051904:Smad8 Protein / Q000235:genetics / Q000378:metabolism
chemical_list: D064451:Hepcidins; D019266:Iron, Dietary; D011134:Polysaccharides; D051898:Smad1 Protein; C497400:Smad1 protein, rat; D051902:Smad5 Protein; C497799:Smad5 protein, rat; D051906:Smad7 Protein; C497808:Smad7 protein, rat; D051904:Smad8 Protein; C109462:Smad9 protein, rat; D048049:Extracellular Signal-Regulated MAP Kinases; D048052:Mitogen-Activated Protein Kinase 3