title: Pheophytin a and chlorophyll a suppress neuroinflammatory responses in lipopolysaccharide and interferon-γ-stimulated BV2 microglia.
abstract: OBJECTIVE
  Microglia-mediated inflammation is associated with pathogenesis of various neuronal disorders. This study investigated inhibitory effects of pheophytin a (PP) and chlorophyll a (CP) on neuroinflammation and underlying cellular mechanisms in microglia cells.
  
  
  METHODS
  BV2 murine microglia cells were stimulated by lipopolysaccharide (LPS, 100 ng/mL) and interferon (IFN)-γ (10 U/mL). The productions of nitric oxide (NO) and expressions of proinflammatory cytokines and chemokines were determined by ELISA and RT-PCR. Western blot and confocal microscopy were applied to analyze activation of transcription factors and mitogen activated protein kinase (MAPK).
  
  
  RESULTS
  PP and CP significantly reduced the levels of NO, tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6 and chemokines including macrophage inhibitory protein (MIP)-1α, macrophage chemoattractant protein (MCP)-1 and IFN-γ inducible protein (IP)-10 in BV2 cells stimulated with LPS and IFN-γ (LI). The nuclear expression of p65 NF-κB was significantly suppressed, which was accompanied by reduced the levels of IFN-β, phospho-STAT-1, and interferon regulatory factor (IRF)-1. Activation of extracellular signal-regulated kinase (ERK) and c-Jun NH2-terminal kinase (JNK) but not p38 MAPK were prominently suppressed by PP and/or CP.
  
  
  CONCLUSIONS
  PP and CP may suppress inflammatory responses by inhibiting NF-κB activation and type I IFN signaling pathway. These result suggested that PP and CP have potential as anti-inflammatory agents for microglia-mediated neuroinflammatory disorders.
authors.affiliation: Laboratory of Pathology, College of Korean Medicine, Daejeon University, Daejeon 300-716, Republic of Korea.
authors.forename: Sunyoung
authors.initials: S
authors.lastname: Park
authors.affiliation: Natural Products Research Institute, Gyeonggi Institute of Science and Technology Promotion, Gyeonggi-do 443-270, Republic of Korea.
authors.forename: Jeong June
authors.initials: JJ
authors.lastname: Choi
authors.affiliation: Laboratory of Pathology, College of Korean Medicine, Daejeon University, Daejeon 300-716, Republic of Korea.
authors.forename: Bo-Kyung
authors.initials: BK
authors.lastname: Park
authors.affiliation: Laboratory of Pathology, College of Korean Medicine, Daejeon University, Daejeon 300-716, Republic of Korea.
authors.forename: Soo Jeong
authors.initials: SJ
authors.lastname: Yoon
authors.affiliation: Laboratory of Pathology, College of Korean Medicine, Daejeon University, Daejeon 300-716, Republic of Korea.
authors.forename: Jung Eun
authors.initials: JE
authors.lastname: Choi
authors.affiliation: Laboratory of Pathology, College of Korean Medicine, Daejeon University, Daejeon 300-716, Republic of Korea. Electronic address: mirimj@dju.kr.
authors.forename: Mirim
authors.initials: M
authors.lastname: Jin
mesh_terms: D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002734:Chlorophyll / Q000494:pharmacology*; D000077194:Chlorophyll A; D018836:Inflammation Mediators / Q000037:antagonists & inhibitors*; D007371:Interferon-gamma / Q000037:antagonists & inhibitors / Q000633:toxicity*; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors / Q000633:toxicity*; D051379:Mice; D017628:Microglia / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D010674:Pheophytins / Q000037:antagonists & inhibitors / Q000494:pharmacology*
chemical_list: D018836:Inflammation Mediators; D008070:Lipopolysaccharides; D010674:Pheophytins; D002734:Chlorophyll; C061694:pheophytin a; D007371:Interferon-gamma; D000077194:Chlorophyll A
keywords: BV2 cell; Chlorophyll a; Microglia; Neuroinflammation; Pheophytin a