title: Microarray analysis of differentially expressed genes engaged in fruit development between table and wine grape.
abstract: Microarray analysis of genes can provide individual gene-expression profiles and new insights for elucidating biological mechanisms responsible for fruit development. To obtain an overall view on expression profiles of metabolism-related genes involved in fruit development of table and wine grapes, a microarray system comprising 15,403 ESTs was used to compare the expressed genes. The expression patterns from the microarray analysis were validated with quantitative real-time polymerase chain reaction analysis of 18 selected genes of interest. During the entire fruit development stage, 2,493 genes exhibited at least 2.0-fold differences in expression levels with 1,244 genes being up-regulated and 1,249 being down-regulated. Following gene ontology analysis, only 929 differentially expressed (including 403 up-regulated and 526 down-regulated) genes were annotated in table and wine grapes. These differentially expressed genes were found to be mainly involved in carbohydrate metabolism, biosynthesis of secondary metabolites as well as energy, lipid and amino acid metabolism via KEGG. Our results provide new insights into the molecular mechanisms and expression profiles of genes in the fruit development stage of table and wine grapes.
authors.affiliation: College of Horticulture, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing, 210095, Jiangsu, People's Republic of China, wxcown@163.com.
authors.forename: Xicheng
authors.initials: X
authors.lastname: Wang
authors.forename: Emrul
authors.initials: E
authors.lastname: Kayesh
authors.forename: Jian
authors.initials: J
authors.lastname: Han
authors.forename: Chonghuai
authors.initials: C
authors.lastname: Liu
authors.forename: Chen
authors.initials: C
authors.lastname: Wang
authors.forename: Changnian
authors.initials: C
authors.lastname: Song
authors.forename: Anjing
authors.initials: A
authors.lastname: Ge
authors.forename: Jinggui
authors.initials: J
authors.lastname: Fang
mesh_terms: D050260:Carbohydrate Metabolism / Q000235:genetics; D004734:Energy Metabolism / Q000235:genetics; D020224:Expressed Sequence Tags; D005638:Fruit / Q000235:genetics*; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D050356:Lipid Metabolism / Q000235:genetics; D046228:Microarray Analysis; D058977:Molecular Sequence Annotation; D027843:Vitis / Q000235:genetics*; D014920:Wine
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