title: Genotoxicity Evaluation of Acephate and Profenofos by the PCR-RFLP Assay.
abstract: OBJECTIVE
  In this study we have evaluated the genotoxic potential of pesticides acephate and profenofos by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay with the mosquito Culex quinquefasciatus taken as experimental model.
  
  
  METHODS
  Second instar larvae were treated with LC20 of each pesticide for 24 h and induced mutations in the sequence of mitochondrial 16S rRNA gene were studied from restriction patterns generated with PacI and PsiI restriction endonucleases.
  
  
  RESULTS
  Variations in the number and size of digested fragments were recorded from treated individuals compared with controls showing that the restriction enzymes created a cut at different locations. In addition, sequences of the 16S gene from control and treated individuals were also used to confirm the RFLP patterns. From the sequence alignment data, it was found that mutations caused the destruction and generation of restriction sites in the gene sequence of treated individuals.
  
  
  CONCLUSIONS
  This study indicates that both the pesticides had significant potential to induce mutations in the 16S gene of Culex quinquefasciatus.
authors.affiliation: Department of Zoology, Punjab University, Chandigarh, India.
authors.forename: Preety
authors.initials: P
authors.lastname: Bhinder
authors.affiliation: Department of Zoology, Punjab University, Chandigarh, India.
authors.forename: Asha
authors.initials: A
authors.lastname: Chaudhry
keywords: Acephate; culex quinquefasciatus; genotoxicity; pcr-rflp; profenofos
references.citation: Hum Exp Toxicol. 2005 Sep;24(9):439-45
references.pmid: 16235732
references.citation: Mutat Res. 2009 Dec 1;671(1-2):84-92
references.pmid: 19800895
references.citation: Toxicol Sci. 1998 Dec;46(2):403-9
references.pmid: 10048144
references.citation: Ecotoxicol Environ Saf. 2010 Sep;73(6):1415-23
references.pmid: 20627310
references.citation: Cytotechnology. 2009 Mar;59(2):73-80
references.pmid: 19404762
references.citation: Neurotoxicology. 2003 Jun;24(3):435-42
references.pmid: 12782108
references.citation: Mutagenesis. 1994 Jul;9(4):319-24
references.pmid: 7968573
references.citation: J Appl Toxicol. 1998 Sep-Oct;18(5):301-5
references.pmid: 9804428
references.citation: Mutagenesis. 2005 Jul;20(4):285-90
references.pmid: 15899934
references.citation: J Biosci. 2000 Dec;25(4):361-6
references.pmid: 11120588
references.citation: Cell Mol Biol (Noisy-le-grand). 2011 Feb 12;57(1):122-8
references.pmid: 21366971
references.citation: Food Chem Toxicol. 2001 Apr;39(4):361-6
references.pmid: 11295482
references.citation: Toxicol Ind Health. 2007 Aug;23(7):439-44
references.pmid: 18536496
references.citation: Nucleic Acids Res. 1990 Nov 25;18(22):6531-5
references.pmid: 1979162
references.citation: Drug Chem Toxicol. 2006;29(3):313-22
references.pmid: 16777709
references.citation: Clin Chim Acta. 2006 Apr;366(1-2):1-13
references.pmid: 16337171