title: [Genotyping and its epidemiological significance on Yunnan Yersinia pestis under Fse I enzyme digestion method].
abstract: OBJECTIVE
  To create the fingerprint library of Yunnan Yersinia pestis by pulse field gel electrophoresis(PFGE)with Fse I enzyme digestion method and to study its epidemiological significance.
  
  
  METHODS
  We used rare cutting restriction enzyme Fse? to digest Yunnan Yersinia pestis strains that were isolated from foci including Rattus flavipectus Plague Focus,Apodemus chevrieri-Eothenomys miletus Plague Focus and Yulong Plague Focus. Fingerprints to Bionumerics software were used for cluster analysis.
  
  
  RESULTS
  30 tested strains were divided into 16 genotypes with the similarity value as 79.8%-100.0% . 16 genotypes wee formed into 4 clusters, with one consisted of only EV76 while the other three belonged to Rattus flavipectus,Apodemus chevrieri- Eothenomys miletus and Yulong clusters, respectively.
  
  
  CONCLUSIONS
  PFGE genotypes of Yunnan Yersinia pestis accorded with its ecotypes and biovars, with clustered regional features. The strains isolated from Yulong showed an unique PFGE type, indicating a new clone group was identified.
authors.affiliation: Provincial Key Laboratory for Plague Control and Prevention, Yunnan Institute for Endemic Diseases Control and Prevention, Dali 671000, China.
authors.forename: Liyuan
authors.initials: L
authors.lastname: Shi
authors.affiliation: School of Public Health, Dali University, Dali, Yunnan Province.
authors.forename: Rui
authors.initials: R
authors.lastname: Ye
authors.affiliation: Provincial Key Laboratory for Plague Control and Prevention, Yunnan Institute for Endemic Diseases Control and Prevention, Dali 671000, China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Dong
authors.affiliation: Provincial Key Laboratory for Plague Control and Prevention, Yunnan Institute for Endemic Diseases Control and Prevention, Dali 671000, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Provincial Key Laboratory for Plague Control and Prevention, Yunnan Institute for Endemic Diseases Control and Prevention, Dali 671000, China.
authors.forename: Guangcan
authors.initials: G
authors.lastname: Yang
authors.affiliation: School of Public Health,Dali University, Dali, Yunnan Province.
authors.forename: Rong
authors.initials: R
authors.lastname: Zhang
authors.affiliation: National Institute for Communicable Disease Control and Prevention and State Key Laboratory for Infectious Diseases Prevention and Control, Chinese Center for Disease Control and Prevention.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Cui
authors.affiliation: National Institute for Communicable Disease Control and Prevention and State Key Laboratory for Infectious Diseases Prevention and Control, Chinese Center for Disease Control and Prevention.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: Provincial Key Laboratory for Plague Control and Prevention, Yunnan Institute for Endemic Diseases Control and Prevention, Dali 671000, China; School of Public Health, Dali University, Dali, Yunnan Province. Email: wp030801@126.com.
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
mesh_terms: D000818:Animals; D002681:China / Q000453:epidemiology; D016000:Cluster Analysis; D016521:Electrophoresis, Gel, Pulsed-Field; D005838:Genotype; D006801:Humans; D010802:Phylogeny; D010930:Plague / Q000453:epidemiology*; D051381:Rats; D015010:Yersinia pestis / Q000235:genetics* / Q000302:isolation & purification