title: PCR for detection of oseltamivir resistance mutation in influenza A(H7N9) virus.
abstract: Sensitive molecular techniques are needed for rapid detection of the R292K oseltamivir-resistant mutant of influenza A(H7/N9) virus strain to monitor its transmission and guide antiviral treatment. We developed a real-time reverse transcription PCR and single nucleotide polymorphism probes to differentiate this mutant strain in mixed virus populations in human specimens.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Song
authors.forename: Wencai
authors.initials: W
authors.lastname: Guan
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.forename: Xiaonan
authors.initials: X
authors.lastname: Zhang
authors.forename: Lei
authors.initials: L
authors.lastname: Xu
authors.forename: Jianhua
authors.initials: J
authors.lastname: Li
authors.forename: Zhenghong
authors.initials: Z
authors.lastname: Yuan
authors.forename: Yunwen
authors.initials: Y
authors.lastname: Hu
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology*; D024882:Drug Resistance, Viral / Q000235:genetics*; D006801:Humans; D064766:Influenza A Virus, H7N9 Subtype / Q000187:drug effects* / Q000235:genetics*; D007251:Influenza, Human / Q000188:drug therapy / Q000453:epidemiology / Q000821:virology; D009154:Mutation; D009439:Neuraminidase / Q000235:genetics; D053139:Oseltamivir / Q000494:pharmacology*; D016133:Polymerase Chain Reaction; D014764:Viral Proteins / Q000235:genetics
chemical_list: D000998:Antiviral Agents; D014764:Viral Proteins; D053139:Oseltamivir; C487630:NA protein, influenza A virus; D009439:Neuraminidase
keywords: H7N9; NA 292K mutant virus; R292K wild-type virus; SNP; influenza; influenza A(H7N9) virus; influenza virus; mutation; neuraminidase; oseltamivir resistance; real-time RT-PCR; real-time reverse transcription PCR; single-nucleotide polymorphism; viruses
references.citation: N Engl J Med. 2013 May 16;368(20):1888-97
references.pmid: 23577628
references.citation: Emerg Infect Dis. 2013;19(9):1521-4
references.pmid: 23965618
references.citation: Nature. 2013 Sep 26;501(7468):551-5
references.pmid: 23842494
references.citation: N Engl J Med. 2013 Jun 13;368(24):2277-85
references.pmid: 23697469
references.citation: J Virol. 2010 Nov;84(22):12069-74
references.pmid: 20826688
references.citation: J Virol Methods. 2010 Jan;163(1):109-15
references.pmid: 19761797
references.citation: Lancet. 2013 Jun 29;381(9885):2273-9
references.pmid: 23726392