title: Synthesis, characterization and biological activities of curcumin nanospheres.
abstract: Curcumin is one of the most versatile compounds obtained from Curcuma longa. The major obstacle in the therapeutic use of curcumin is its aqueous solubility. To enhance its aqueous solubility and biological activities, we prepared curcumin nanospheres (CNSs) by wet milling-solvent evaporation technique without any surfactants. In this study, we have focused on the synthesis, characterization and biological effects of CNSs. DLS and SEM analyses showed 50-80 nm spherical shaped CNSs with a zeta potential of -31.65 mV. FTIR revealed that there were no structural changes to CNSs. Antibacterial and antifungal studies proved that CNSs were much more effective than curcumin against Escherichia coil, Staphylococcus aureus and Candida albicans. Antioxidant activity of CNSs showed promising result for therapeutic applications. The in vitro anti-inflammatory studies proved that CNSs possessed enhanced anti-inflammatory effect against protein denaturation. Cytotoxicity and uptake of CNSs showed more toxicity on cancer cells (T47D, MG63, A375) sparing normal HDF and IEC cell lines. Skin permeation studies showed CNSs retained at different layers of pig skin. These results give clear evidence for their use against microbial and fungal skin infections as well as cancer treatment.
authors.forename: T R
authors.initials: TR
authors.lastname: Arunraj
authors.forename: N
authors.initials: N
authors.lastname: Sanoj Rejinold
authors.forename: Sabitha
authors.initials: S
authors.lastname: Mangalathillam
authors.forename: Soumya
authors.initials: S
authors.lastname: Saroj
authors.forename: Raja
authors.initials: R
authors.lastname: Biswas
authors.forename: R
authors.initials: R
authors.lastname: Jayakumar
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects / Q000254:growth & development; D001777:Blood Coagulation / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D015169:Colony Count, Microbial; D003474:Curcumin / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D004055:Differential Thermal Analysis; D005658:Fungi / Q000187:drug effects / Q000254:growth & development; D006801:Humans; D066298:In Vitro Techniques; D008422:Materials Testing; D008826:Microbial Sensitivity Tests; D054118:Nanospheres / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D010539:Permeability / Q000187:drug effects; D012867:Skin / Q000187:drug effects / Q000382:microbiology; D017550:Spectroscopy, Fourier Transform Infrared; D055672:Static Electricity; D034421:Sus scrofa; D013818:Thermogravimetry; D014961:X-Ray Diffraction
chemical_list: D000890:Anti-Infective Agents; D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D003474:Curcumin