title: Antagonism by salvianolic acid B of lipopolysaccharide-induced disseminated intravascular coagulation in rabbits.
abstract: The aim of the present study was to investigate the effects of salvianolic acid B on lipopolysaccharide (LPS)-induced disseminated intravascular coagulation (DIC) in rabbits. Continuous infusion of LPS was used to induce a DIC model in rabbits. Treatment with salvianolic acid B (1, 3 or 6 mg/kg) was started simultaneously with LPS infusion (0.5 mg/kg LPS in 60 mL saline; 10 mL/h over a period of 6 h) through the contralateral marginal ear vein. Activated partial thromboplastin time (APTT), prothrombin time (PT), platelet count and fibrinogen concentration were determined, as were plasma levels of fibrin-fibrinogen degradation products (FDP), alanine aminotransferase (ALT), blood urea nitrogen (BUN), protein C activity, antithrombin III (ATIII) and tumour necrosis factor (TNF)-α concentration. The gradual impairment of haemostatic parameters was induced by continuous infusion of LPS. There were marked increases in APTT, PT, BUN, ALT and plasma TNF-α and marked decreases in the platelet count, fibrinogen, FDP, protein C and ATIII. The intravenous administration of 1, 3 or 6 mg/kg salvianolic acid B attenuated the increases in APTT, PT, BUN, ALT and plasma TNF-α and the decreases in fibrinogen, platelet, FDP, protein C and ATIII induced by LPS infusion. These observations indicate that salvianolic acid B has an effect against LPS-induced DIC in rabbits.
authors.affiliation: Department of Developmental and Regenerative Biology, Ji-nan University, Guangzhou, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Wu
authors.forename: Jian-Nan
authors.initials: JN
authors.lastname: Li
authors.forename: Zhi-Quan
authors.initials: ZQ
authors.lastname: Bai
authors.forename: Xi
authors.initials: X
authors.lastname: Lin
mesh_terms: D000818:Animals; D001572:Benzofurans / Q000627:therapeutic use*; D004211:Disseminated Intravascular Coagulation / Q000139:chemically induced* / Q000150:complications / Q000188:drug therapy* / Q000473:pathology; D005786:Gene Expression Regulation / Q000187:drug effects; D007674:Kidney Diseases / Q000188:drug therapy / Q000209:etiology; D008070:Lipopolysaccharides / Q000633:toxicity*; D008107:Liver Diseases / Q000188:drug therapy / Q000209:etiology; D008297:Male; D011817:Rabbits; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism; D053630:Depsides
chemical_list: D001572:Benzofurans; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; C076944:salvianolic acid B; D053630:Depsides
keywords: disseminated intravascular coagulation; heparin; lipopolysaccharide; salvianolic acid B; tumour necrosis factor α