title: Human serum albumin Cys34 oxidative modifications following infiltration in the carotid atherosclerotic plaque.
abstract: OBJECTIVE
  To evaluate if the prooxidant environment present in atherosclerotic plaque may oxidatively modify filtered albumin.
  
  
  METHODS
  Fluorescein-5-maleimide labelled plasma samples and plaque extracts from 27 patients who had undergone carotid endarterectomy were analysed through nonreducing SDS-PAGE for albumin-Cys(34) oxidation. Furthermore, degree and pattern of S-thiolation in both circulating and plaque-filtered albumin were assayed.
  
  
  RESULTS
  Albumin filtered in the atherosclerotic plaque showed higher levels of Cys(34) oxidative modifications than the corresponding circulating form as well as different patterns of S-thiolation.
  
  
  CONCLUSIONS
  Data indicate that the circulating albumin, once filtered in plaque, undergoes Cys(34) oxidative modifications and demonstrate for the first time that albumin is a homocysteine and cysteinylglycine vehicle inside the plaque environment.
authors.affiliation: Dipartimento di Scienze Biomediche, University of Sassari, Via Muroni 25, 07100 Sassari, Italy.
authors.forename: Antonio Junior
authors.identifier: 0000-0002-8356-894X
authors.initials: AJ
authors.lastname: Lepedda
authors.affiliation: Dipartimento di Scienze Biomediche, University of Sassari, Via Muroni 25, 07100 Sassari, Italy.
authors.forename: Angelo
authors.initials: A
authors.lastname: Zinellu
authors.affiliation: Dipartimento di Scienze Biomediche, University of Sassari, Via Muroni 25, 07100 Sassari, Italy.
authors.forename: Gabriele
authors.identifier: 0000-0002-9268-9436
authors.initials: G
authors.lastname: Nieddu
authors.affiliation: Dipartimento di Scienze Biomediche, University of Sassari, Via Muroni 25, 07100 Sassari, Italy.
authors.forename: Pierina
authors.initials: P
authors.lastname: De Muro
authors.affiliation: Dipartimento di Scienze Biomediche, University of Sassari, Via Muroni 25, 07100 Sassari, Italy.
authors.forename: Ciriaco
authors.initials: C
authors.lastname: Carru
authors.affiliation: Centro Cardiologico "F. Monzino", IRCCS, 20138 Milano, Italy.
authors.forename: Rita
authors.initials: R
authors.lastname: Spirito
authors.affiliation: Centro Cardiologico "F. Monzino", IRCCS, 20138 Milano, Italy.
authors.forename: Anna
authors.initials: A
authors.lastname: Guarino
authors.affiliation: Servizio di Chirurgia Vascolare, Clinica Chirurgica Generale, University of Sassari, 07100 Sassari, Italy.
authors.forename: Franco
authors.identifier: 0000-0003-1609-9968
authors.initials: F
authors.lastname: Piredda
authors.affiliation: Dipartimento di Scienze Biomediche, University of Sassari, Via Muroni 25, 07100 Sassari, Italy.
authors.forename: Marilena
authors.identifier: 0000-0001-9207-5787
authors.initials: M
authors.lastname: Formato
mesh_terms: D002138:Calibration; D002339:Carotid Arteries / Q000473:pathology*; D003545:Cysteine / Q000378:metabolism*; D005452:Fluoresceins / Q000378:metabolism; D006801:Humans; D007553:Isotope Labeling; D008970:Molecular Weight; D010084:Oxidation-Reduction; D058226:Plaque, Atherosclerotic / Q000378:metabolism* / Q000473:pathology*; D012709:Serum Albumin / Q000378:metabolism*; D013438:Sulfhydryl Compounds / Q000097:blood
chemical_list: D005452:Fluoresceins; D012709:Serum Albumin; D013438:Sulfhydryl Compounds; C037041:fluorescein 5-maleimide; D003545:Cysteine
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