title: Y-chromosome status identification suggests a recipient origin of posttransplant non-small cell lung carcinomas: chromogenic in situ hybridization analysis.
abstract: Owing to the need of lifelong immunosuppression, solid-organ transplant recipients are known to have an increased risk of posttransplant malignancies including lung cancer. Posttransplant neoplastic transformation of donor-derived cells giving rise to hematopoietic malignancies, Kaposi sarcoma, and basal cell carcinoma in nongraft tissues has been reported. The goal of this study was to assess the cell origin (donor versus recipient derived) of posttransplant non-small cell lung carcinomas (NSCLCs) in kidney and heart transplant recipients. An institutional database search identified 2557 kidney and heart transplant recipients in 8 consecutive years. Among this cohort, 20 (0.8%) renal and 18 (0.7%) heart transplant recipients developed NSCLC. The study cohort comprised 6 of 38 NSCLCs arising in donor-recipient sex-mismatched transplant patients. The tumor cell origin was evaluated by chromogenic in situ hybridization with Y-chromosome probe on formalin-fixed, paraffin-embedded tissues. Y-chromosome was identified in 97% ± 1% (range from 92% to 99%) of all types of nucleated cells in male control tissues. In all 5 NSCLCs from male recipients of female donor organ, Y-chromosome was identified in 97% ± 2% (range from 92% to 100%) of tumor cells, statistically equivalent to normal control (P < .001). No Y-chromosome was identified in NSCLC cells from a female recipient of male kidney. These findings suggest a recipient derivation of NSCLC arising in kidney and heart transplant recipients. A combination of histologic evaluation and chromogenic in situ hybridization with Y-chromosome analysis allows reliable determination of tissue origin in sex-mismatched solid-organ transplant recipients and may aid in management of posttransplant malignancy in such cases.
authors.affiliation: Department of Pathology, The Ohio State University Wexner Medical Center, Columbus, OH 43210.
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.affiliation: Department of Pathology, The Ohio State University Wexner Medical Center, Columbus, OH 43210.
authors.forename: Sergey V
authors.initials: SV
authors.lastname: Brodsky
authors.affiliation: Department of Pathology, The Ohio State University Wexner Medical Center, Columbus, OH 43210.
authors.forename: Weiqiang
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Department of Medicine, The Ohio State University Wexner Medical Center, Columbus, OH 43210.
authors.forename: Gregory A
authors.initials: GA
authors.lastname: Otterson
authors.affiliation: Department of Medicine, The Ohio State University Wexner Medical Center, Columbus, OH 43210.
authors.forename: Miguel
authors.initials: M
authors.lastname: Villalona-Calero
authors.affiliation: Department of Pathology, The Ohio State University Wexner Medical Center, Columbus, OH 43210.
authors.forename: Anjali A
authors.initials: AA
authors.lastname: Satoskar
authors.affiliation: Department of Medicine, The Ohio State University Wexner Medical Center, Columbus, OH 43210.
authors.forename: Ayesha
authors.initials: A
authors.lastname: Hasan
authors.affiliation: Department of Surgery, The Ohio State University Wexner Medical Center, Columbus, OH 43210.
authors.forename: Ronald
authors.initials: R
authors.lastname: Pelletier
authors.affiliation: Department of Pathology, The Ohio State University Wexner Medical Center, Columbus, OH 43210.
authors.forename: Iouri
authors.initials: I
authors.lastname: Ivanov
authors.affiliation: Department of Surgery, The Ohio State University Wexner Medical Center, Columbus, OH 43210.
authors.forename: Patrick
authors.initials: P
authors.lastname: Ross
authors.affiliation: Department of Pathology, The Ohio State University Wexner Medical Center, Columbus, OH 43210.
authors.forename: Tibor
authors.initials: T
authors.lastname: Nadasdy
authors.affiliation: Department of Pathology, The Ohio State University Wexner Medical Center, Columbus, OH 43210. Electronic address: Konstantin.Shilo@OSUMC.edu.
authors.forename: Konstantin
authors.initials: K
authors.lastname: Shilo
mesh_terms: D000368:Aged; D002289:Carcinoma, Non-Small-Cell Lung / Q000209:etiology* / Q000235:genetics; D041322:Chromosomes, Human, Y; D005260:Female; D016027:Heart Transplantation / Q000009:adverse effects*; D006801:Humans; D007165:Immunosuppression Therapy / Q000009:adverse effects; D016030:Kidney Transplantation / Q000009:adverse effects*; D008297:Male; D008875:Middle Aged; D014019:Tissue Donors
keywords: Chromogenic in situ hybridization for Y-chromosome; Post–solid-organ transplantation lung cancer
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