title: Quantifying the volume of single cells continuously using a microfluidic pressure-driven trap with media exchange.
abstract: We demonstrate a microfluidic device capable of tracking the volume of individual cells by integrating an on-chip volume sensor with pressure-activated cell trapping capabilities. The device creates a dynamic trap by operating in feedback; a cell is periodically redirected back and forth through a microfluidic volume sensor (Coulter principle). Sieve valves are positioned on both ends of the sensing channel, creating a physical barrier which enables media to be quickly exchanged while keeping a cell firmly in place. The volume of individual Saccharomyces cerevisiae cells was tracked over entire growth cycles, and the ability to quickly exchange media was demonstrated.
authors.affiliation: Department of Physics, University of Ottawa, Ottawa, Ontario K1N 6N5, Canada.
authors.forename: Jason
authors.initials: J
authors.lastname: Riordon
authors.affiliation: Department of Physics, University of Ottawa, Ottawa, Ontario K1N 6N5, Canada.
authors.forename: Michael
authors.initials: M
authors.lastname: Nash
authors.affiliation: Department of Physics, University of Ottawa, Ottawa, Ontario K1N 6N5, Canada.
authors.forename: Wenyang
authors.initials: W
authors.lastname: Jing
authors.affiliation: Department of Physics, University of Ottawa, Ottawa, Ontario K1N 6N5, Canada ; Ottawa-Carleton Institute for Biomedical Engineering, Ottawa, Ontario K1N 6N5, Canada.
authors.forename: Michel
authors.initials: M
authors.lastname: Godin
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