title: Phosphorylation of highly conserved serine residues in the influenza A virus nuclear export protein NEP plays a minor role in viral growth in human cells and mice.
abstract: Phosphorylation at the highly conserved serine residues S23 to S25 in the nuclear export protein (NEP) of influenza A viruses was suspected to regulate its nuclear export activity or polymerase activity-enhancing function. Mutation of these phosphoacceptor sites to either alanine or aspartic acid showed only a minor effect on both activities but revealed the presence of other phosphoacceptor sites that might be involved in regulating NEP activity.
authors.affiliation: Institute for Virology, University Medical Center Freiburg, Freiburg, Germany University of Freiburg, Freiburg, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Reuther
authors.affiliation: Institute for Virology, University Medical Center Freiburg, Freiburg, Germany University of Freiburg, Freiburg, Germany.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Giese
authors.affiliation: Institute for Virology, University Medical Center Freiburg, Freiburg, Germany University of Freiburg, Freiburg, Germany.
authors.forename: Veronika
authors.initials: V
authors.lastname: Götz
authors.affiliation: Institute for Virology, University Medical Center Freiburg, Freiburg, Germany.
authors.forename: David
authors.initials: D
authors.lastname: Riegger
authors.affiliation: Institute for Virology, University Medical Center Freiburg, Freiburg, Germany martin.schwemmle@uniklinik-freiburg.de.
authors.forename: Martin
authors.initials: M
authors.lastname: Schwemmle
mesh_terms: D021581:Active Transport, Cell Nucleus / Q000502:physiology*; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000818:Animals; D004279:DNA, Viral / Q000235:genetics; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D009980:Influenza A virus / Q000235:genetics / Q000254:growth & development* / Q000472:pathogenicity; D051379:Mice; D008969:Molecular Sequence Data; D009976:Orthomyxoviridae Infections / Q000378:metabolism / Q000821:virology*; D010766:Phosphorylation; D017386:Sequence Homology, Amino Acid; D012694:Serine / Q000378:metabolism*; D017361:Viral Nonstructural Proteins / Q000378:metabolism*; D014774:Virulence; D014779:Virus Replication
chemical_list: D004279:DNA, Viral; D017361:Viral Nonstructural Proteins; D012694:Serine; D004259:DNA-Directed DNA Polymerase
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