title: Electrochemical evaluation of troponin T imprinted polymer receptor.
abstract: The selective detection and quantification of macromolecular targets is a fundamental biological mechanism in nature. Molecularly imprinted polymers (MIPs) have been identified as one of the most promising synthetic alternatives to bioreceptors. However, expanding this methodology towards selective recognition of bulky templates such as proteins appears to be extremely challenging due to problems associated with removal of the template from the polymeric network. In this study, polymer imprinted with troponin T (TnT) was assessed using electrochemical methods and the influence of various extraction methods, including conventional immersion extraction, thermal annealing and ultrasonic-assisted extraction, on the binding characteristics of the troponin-to-imprinted polymer receptor was elucidated. Cyclic voltammetric deposition of o-phenylenediamine (o-PD) film in the presence of TnT as a template was performed in acetate buffer (0.5 M, pH 5.2) on a gold substrate. Solvent extraction of the target molecule was optimised and followed by subsequent washing with water. The electrochemistry of a ferro/ferricyanide probe was used to characterise the TnT MIP receptor film. The incubation of the TnT MIP receptor-modiﬁed electrode with respect to TnT concentration resulted in a suppression of the ferro/ferricyanide redox current. The dissociation constant (KD) was calculated using a two-site model of template affinity for the TnT MIP receptor. The synthetic TnT MIP receptor had high affinity for TnT with a KD of 2.3×10(-13) M.
authors.affiliation: Biosensors and Bioelectronics Centre, Department of Physics, Chemistry and Biology (IFM), Linköping University, S-58183 Linköping, Sweden; Department of Chemistry, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran.
authors.forename: Najmeh
authors.initials: N
authors.lastname: Karimian
authors.affiliation: Biosensors and Bioelectronics Centre, Department of Physics, Chemistry and Biology (IFM), Linköping University, S-58183 Linköping, Sweden.
authors.forename: Anthony P F
authors.initials: AP
authors.lastname: Turner
authors.affiliation: Biosensors and Bioelectronics Centre, Department of Physics, Chemistry and Biology (IFM), Linköping University, S-58183 Linköping, Sweden. Electronic address: ashutosh.tiwari@liu.se.
authors.forename: Ashutosh
authors.initials: A
authors.lastname: Tiwari
mesh_terms: D015374:Biosensing Techniques / Q000379:methods; D055664:Electrochemical Techniques / Q000379:methods*; D006801:Humans; D054802:Molecular Imprinting; D011108:Polymers / Q000737:chemistry*; D020107:Troponin T / Q000032:analysis*
chemical_list: D011108:Polymers; D020107:Troponin T
keywords: Electrochemical study; Imprinted polymer; Synthetic receptor; Template affinity; Troponin T