title: Triterpenoid saponins from the roots of two Gypsophila species.
abstract: Two triterpenoid saponins with two known ones have been isolated from the roots of Gypsophila arrostii var. nebulosa, and two new ones from the roots of Gypsophila bicolor. Their structures were established by extensive NMR and mass spectroscopic techniques as 3-O-β-d-galactopyranosyl-(1→2)-[β-d-xylopyranosyl-(1→3)]-β-d-glucuronopyranosylquillaic acid 28-O-β-d-xylopyranosyl-(1→4)-[β-d-glucopyranosyl-(1→3)]-α-l-rhamnopyranosyl-(1→2)-[β-d-glucopyranosyl-(1→4)]-β-d-fucopyranosyl ester (1), 3-O-β-d-galactopyranosyl-(1→2)-[β-d-xylopyranosyl-(1→3)]-β-d-glucuronopyranosylgypsogenin 28-O-β-d-xylopyranosyl-(1→4)-[β-d-glucopyranosyl-(1→3)]-α-l-rhamnopyranosyl-(1→2)-[β-d-glucopyranosyl-(1→4)]-β-d-fucopyranosyl ester (2), 3-O-β-d-galactopyranosyl-(1→2)-[β-d-xylopyranosyl-(1→3)]-β-d-glucuronopyranosylgypsogenin 28-O-β-d-xylopyranosyl-(1→3)-β-d-xylopyranosyl-(1→4)-α-l-rhamnopyranosyl-(1→2)-[(4-O-acetyl)-β-d-quinovopyranosyl-(1→4)]-β-d-fucopyranosyl ester (3), gypsogenic acid 28-O-β-d-glucopyranosyl-(1→3)-{6-O-[3-hydroxy-3-methylglutaryl]-β-d-glucopyranosyl-(1→6)}-β-d-galactopyranosyl ester (4). Three compounds were evaluated against one human colon cancer cell line SW480 and one rat cardiomyoblast cell line H9c2.
authors.affiliation: Laboratoire de Pharmacognosie, EA 4267, FDE/UFC, UFR Pharmacie, Université de Bourgogne, 7, Bd Jeanne d'Arc, BP 87900, 21079 Dijon Cedex, France.
authors.forename: David
authors.initials: D
authors.lastname: Pertuit
authors.affiliation: Mugla University, Saglık Hizmetleri Meslek Yuksekokulu, Ulusal Egemenlik Cad. No:9 Marmaris, Mugla, Turkey.
authors.forename: Sibel
authors.initials: S
authors.lastname: Avunduk
authors.affiliation: Laboratoire de Pharmacognosie, EA 4267, FDE/UFC, UFR Pharmacie, Université de Bourgogne, 7, Bd Jeanne d'Arc, BP 87900, 21079 Dijon Cedex, France.
authors.forename: Anne-Claire
authors.initials: AC
authors.lastname: Mitaine-Offer
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka 812-8582, Japan.
authors.forename: Tomofumi
authors.initials: T
authors.lastname: Miyamoto
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka 812-8582, Japan.
authors.forename: Chiaki
authors.initials: C
authors.lastname: Tanaka
authors.affiliation: Universität Siegen, Organische Chemie II, Naturwissenschaftlich-Technische Fakultät, Adolf-Reichwein-Str. 2, D-57076 Siegen, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Paululat
authors.affiliation: Cohiro, UFR Médecine, 7, Bd Jeanne d'Arc, BP 87900, 21079 Dijon Cedex, France.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Delemasure
authors.affiliation: Cohiro, UFR Médecine, 7, Bd Jeanne d'Arc, BP 87900, 21079 Dijon Cedex, France.
authors.forename: Patrick
authors.initials: P
authors.lastname: Dutartre
authors.affiliation: Laboratoire de Pharmacognosie, EA 4267, FDE/UFC, UFR Pharmacie, Université de Bourgogne, 7, Bd Jeanne d'Arc, BP 87900, 21079 Dijon Cedex, France. Electronic address: m-a.lacaille-dubois@u-bourgogne.fr.
authors.forename: Marie-Aleth
authors.initials: MA
authors.lastname: Lacaille-Dubois
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D029748:Caryophyllaceae / Q000737:chemistry*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D008968:Molecular Conformation; D018517:Plant Roots / Q000737:chemistry*; D051381:Rats; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D013045:Species Specificity; D013237:Stereoisomerism; D013329:Structure-Activity Relationship; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D012503:Saponins; D014315:Triterpenes
keywords: 2D NMR; Caryophyllaceae; Gypsophila arrostii; Gypsophila bicolor; Triterpenoid saponins