title: [A modified approach to establishing a rat model of liver cancer with concurrent cirrhosis].
abstract: OBJECTIVE
  To establish a modified rat model of liver cancer with concurrent cirrhosis for the study of carcinogenesis characteristics and drug intervention of liver cancer.
  
  
  METHODS
  Fifty male Wistar rats weighing 100-120 g were randomly divided into normal control group (20 rats) and model group (30 rats). In the model group, the rats were subjected to intraperitoneal injection of 50 mg/kg DEN N-diethylnitrosamine (DEN) twice a week for 4 consecutive weeks, followed then by weekly injections for another 10 weeks. The control rats received injections of 0.1 ml saline in the same manner. At 2, 4, 8, 12, 14, and 18 weeks, 3 rats from each group were sacrificed for assessing tumor formation and liver cirrhosis.
  
  
  RESULTS
  Liver cancer with concurrent cirrhosis was induced successfully after 14 weeks of DEN injections. At the 14th week, 3 out of the 5 rats were found to have cirrhosis and LC, and at the 18th week, all the 3 rats examined had cirrhosis and liver cancer. The total carcinogenesis rate in the rats was 75% at 18 weeks with an overall mortality of 33%.
  
  
  CONCLUSIONS
  This approach to establishing rat models of liver cancer with concurrent cirrhosis requires simple operation, shortens the time of carcinogenesis, and ensures a high success rate of carcinogenesis and a low mortality rate. The carcinogenesis characteristics in this model are similar to those in human.
authors.affiliation: Second Department of Hepatobiliary Surgery, Zhujiang Hospital, Guangzhou 510282, China.E-mail: santcruss@163.com.
authors.forename: Chenjie
authors.initials: C
authors.lastname: Zhou
authors.forename: Xumeng
authors.initials: X
authors.lastname: Gong
authors.forename: Liquan
authors.initials: L
authors.lastname: Cai
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Yi
authors.initials: Y
authors.lastname: Gao
mesh_terms: D000818:Animals; D008106:Liver Cirrhosis, Experimental / Q000150:complications / Q000473:pathology; D008114:Liver Neoplasms, Experimental / Q000209:etiology / Q000473:pathology; D008297:Male; D051381:Rats; D017208:Rats, Wistar