title: PhiC31 integrase induces efficient site-specific recombination in the Capra hircus genome.
abstract: Streptomyces phage φC31 integrase induces efficient site-specific recombination capable of integrating exogenous genes at pseudo attP sites in human, mouse, rat, rabbit, sheep, Drosophila, and bovine genomes. However, the φC31-mediated recombination between attB and the corresponding pseudo attP sites has not been investigated in Capra hircus. Here, we identified eight pseudo attP sites located in the intron or intergenic regions of the C. hircus genome, and demonstrated different levels of foreign gene expression after φC31 integrase-mediated integration. These pseudo attP sites share similar sequences with each other and with pseudo attP sites in other mammalian genomes, and these are associated with a neighboring consensus motif found in other genomes. The application of the φC31 integrase system in C. hircus provides a new option for genetic engineering of this economically important goat species.
authors.affiliation: 1 Shanghai Institute of Medical Genetics, Shanghai Children's Hospital, Shanghai Jiao Tong University , Shanghai, People's Republic of China .
authors.forename: Haiyan
authors.initials: H
authors.lastname: Ma
authors.forename: Qingwen
authors.initials: Q
authors.lastname: Ma
authors.forename: Yao
authors.initials: Y
authors.lastname: Lu
authors.forename: Juan
authors.initials: J
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Hu
authors.forename: Zhijuan
authors.initials: Z
authors.lastname: Gong
authors.forename: Linlin
authors.initials: L
authors.lastname: Cai
authors.forename: Ying
authors.initials: Y
authors.lastname: Huang
authors.forename: Shu-Zhen
authors.initials: SZ
authors.lastname: Huang
authors.forename: Fanyi
authors.initials: F
authors.lastname: Zeng
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001287:Attachment Sites, Microbiological; D001435:Bacteriophages / Q000201:enzymology; D001483:Base Sequence; D002478:Cells, Cultured; D016384:Consensus Sequence; D005818:Genetic Engineering; D016678:Genome; D006041:Goats / Q000235:genetics*; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D019426:Integrases / Q000502:physiology*; D011995:Recombination, Genetic; D013302:Streptomyces / Q000821:virology
chemical_list: D049452:Green Fluorescent Proteins; D019426:Integrases
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