title: The transcription factors Tec1 and Ste12 interact with coregulators Msa1 and Msa2 to activate adhesion and multicellular development.
abstract: In Saccharomyces cerevisiae and related yeast species, the TEA transcription factor Tec1, together with a second transcription factor, Ste12, controls development, including cell adhesion and filament formation. Tec1-Ste12 complexes control target genes through Tec1 binding sites (TEA consensus sequences [TCSs]) that can be further combined with Ste12 binding sites (pheromone response elements [PREs]) for cooperative DNA binding. The activity of Tec1-Ste12 complexes is known to be under negative control of the Dig1 and Dig2 (Dig1/2) transcriptional corepressors that confer regulation by upstream signaling pathways. Here, we found that Tec1 and Ste12 can associate with the transcriptional coregulators Msa1 and Msa2 (Msa1/2), which were previously found to associate with the cell cycle transcription factor complexes SBF (Swi4/Swi6 cell cycle box binding factor) and MBF (Mbp1/Swi6 cell cycle box binding factor) to control G1-specific transcription. We further show that Tec1-Ste12-Msa1/2 complexes (i) do not contain Swi4 or Mbp1, (ii) assemble at single TCSs or combined TCS-PREs in vitro, and (iii) coregulate genes involved in adhesive and filamentous growth by direct promoter binding in vivo. Finally, we found that, in contrast to Dig proteins, Msa1/2 seem to act as coactivators that enhance the transcriptional activity of Tec1-Ste12. Taken together, our findings add an additional layer of complexity to our understanding of the control mechanisms exerted by the evolutionarily conserved TEA domain and Ste12-like transcription factors.
authors.affiliation: Department of Genetics, Philipps-Universität, Marburg, Germany LOEWE Center for Synthetic Microbiology, Marburg, Germany.
authors.forename: Julia
authors.initials: J
authors.lastname: van der Felden
authors.affiliation: Department of Genetics, Philipps-Universität, Marburg, Germany LOEWE Center for Synthetic Microbiology, Marburg, Germany.
authors.forename: Sarah
authors.initials: S
authors.lastname: Weisser
authors.affiliation: Department of Genetics, Philipps-Universität, Marburg, Germany LOEWE Center for Synthetic Microbiology, Marburg, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Brückner
authors.affiliation: Department of Physics, Philipps-Universität, Marburg, Germany LOEWE Center for Synthetic Microbiology, Marburg, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Lenz
authors.affiliation: Department of Genetics, Philipps-Universität, Marburg, Germany LOEWE Center for Synthetic Microbiology, Marburg, Germany moesch@staff.uni-marburg.de.
authors.forename: Hans-Ulrich
authors.initials: HU
authors.lastname: Mösch
mesh_terms: D002448:Cell Adhesion; D018797:Cell Cycle Proteins / Q000378:metabolism; D004271:DNA, Fungal / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D008954:Models, Biological; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism; D020798:Two-Hybrid System Techniques
chemical_list: D018797:Cell Cycle Proteins; D004271:DNA, Fungal; D004268:DNA-Binding Proteins; C529154:Msa1 protein, S cerevisiae; C489241:STE12 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; C069926:TEC1 protein, S cerevisiae; D014157:Transcription Factors
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