title: Latitudinal variation in the degree of crassulacean acid metabolism in Puya chilensis.
abstract: Crassulacean acid metabolism (CAM) is a photosynthetic pathway found in many plant species from arid and semiarid environments. Few studies aiming to characterise plant species as CAM or C3 account for inter-population differences in photosynthetic pathway, often relying on samples taken from herbarium material and/or a single plant or population. This may be especially problematic for species growing under contrasting climate conditions, as is the case for species with a wide geographic range. We used Puya chilensis, a species previously reported as CAM and C3, to study among-population variation in expression of the CAM pathway within its distribution range, which spans a significant climate gradient. We carried out a wide sampling scheme, including five populations and a combination of analytical methods (quantification of nocturnal acidification and stable isotope measurements). The study populations of P. chilensis encompass the entire latitudinal distribution range, from semi-arid to temperate oceanic climates. Our results indicate that CAM decreased with latitude. However, even in the southern (wetter) populations, where δ13C values were indicative of C3 metabolism, we found some nocturnal acidification. We stress the value of using two methods along with the use of samples from different populations, as this allows more reliable conclusions on the photosynthetic pathway for 'probable' CAM species that face varying climate conditions within their distribution ranges.
authors.affiliation: Departamento de Botánica, Universidad de Concepción, Concepción, Chile.
authors.forename: I M
authors.initials: IM
authors.lastname: Quezada
authors.forename: G
authors.initials: G
authors.lastname: Zotz
authors.forename: E
authors.initials: E
authors.lastname: Gianoli
mesh_terms: D031229:Bromeliaceae / Q000378:metabolism* / Q000502:physiology*; D002245:Carbon Dioxide / Q000378:metabolism*; D055864:Droughts; D019083:Mediterranean Region
chemical_list: D002245:Carbon Dioxide
keywords: Bromeliaceae; central Chile; climate gradient; crassulacean acid metabolism; drought tolerance; mediterranean climate