title: Identification of novel and conserved microRNAs related to drought stress in potato by deep sequencing.
abstract: MicroRNAs (miRNAs) are a group of small, non-coding RNAs that play important roles in plant growth, development and stress response. There have been an increasing number of investigations aimed at discovering miRNAs and analyzing their functions in model plants (such as Arabidopsis thaliana and rice). In this research, we constructed small RNA libraries from both polyethylene glycol (PEG 6,000) treated and control potato samples, and a large number of known and novel miRNAs were identified. Differential expression analysis showed that 100 of the known miRNAs were down-regulated and 99 were up-regulated as a result of PEG stress, while 119 of the novel miRNAs were up-regulated and 151 were down-regulated. Based on target prediction, annotation and expression analysis of the miRNAs and their putative target genes, 4 miRNAs were identified as regulating drought-related genes (miR811, miR814, miR835, miR4398). Their target genes were MYB transcription factor (CV431094), hydroxyproline-rich glycoprotein (TC225721), quaporin (TC223412) and WRKY transcription factor (TC199112), respectively. Relative expression trends of those miRNAs were the same as that predicted by Solexa sequencing and they showed a negative correlation with the expression of the target genes. The results provide molecular evidence for the possible involvement of miRNAs in the process of drought response and/or tolerance in the potato plant.
authors.affiliation: Gansu Key Laboratory of Crop Genetic and Germplasm Enhancement, Gansu Provincial Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou, People's Republic of China; College of Life Science and Technology, Gansu Agricultural University, Lanzhou, People's Republic of China.
authors.forename: Ning
authors.initials: N
authors.lastname: Zhang
authors.affiliation: Gansu Key Laboratory of Crop Genetic and Germplasm Enhancement, Gansu Provincial Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou, People's Republic of China.
authors.forename: Jiangwei
authors.initials: J
authors.lastname: Yang
authors.affiliation: Gansu Key Laboratory of Crop Genetic and Germplasm Enhancement, Gansu Provincial Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou, People's Republic of China; College of Life Science and Technology, Gansu Agricultural University, Lanzhou, People's Republic of China.
authors.forename: Zemin
authors.initials: Z
authors.lastname: Wang
authors.affiliation: College of Life Science and Technology, Gansu Agricultural University, Lanzhou, People's Republic of China.
authors.forename: Yikai
authors.initials: Y
authors.lastname: Wen
authors.affiliation: Gansu Key Laboratory of Crop Genetic and Germplasm Enhancement, Gansu Provincial Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou, People's Republic of China; College of Life Science and Technology, Gansu Agricultural University, Lanzhou, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.affiliation: Gansu Key Laboratory of Crop Genetic and Germplasm Enhancement, Gansu Provincial Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou, People's Republic of China; College of Life Science and Technology, Gansu Agricultural University, Lanzhou, People's Republic of China.
authors.forename: Wenhui
authors.initials: W
authors.lastname: He
authors.affiliation: Gansu Key Laboratory of Crop Genetic and Germplasm Enhancement, Gansu Provincial Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou, People's Republic of China.
authors.forename: Bailin
authors.initials: B
authors.lastname: Liu
authors.affiliation: Gansu Key Laboratory of Crop Genetic and Germplasm Enhancement, Gansu Provincial Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou, People's Republic of China; College of Life Science and Technology, Gansu Agricultural University, Lanzhou, People's Republic of China.
authors.forename: Huaijun
authors.initials: H
authors.lastname: Si
authors.affiliation: Gansu Key Laboratory of Crop Genetic and Germplasm Enhancement, Gansu Provincial Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou, People's Republic of China.
authors.forename: Di
authors.initials: D
authors.lastname: Wang
mesh_terms: D019295:Computational Biology; D055864:Droughts; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D059014:High-Throughput Nucleotide Sequencing; D035683:MicroRNAs / Q000235:genetics*; D058977:Molecular Sequence Annotation; D011198:Solanum tuberosum / Q000235:genetics*; D013312:Stress, Physiological / Q000235:genetics*
chemical_list: D035683:MicroRNAs
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