title: Anti-inflammatory activity of N-butanol extract from Ipomoea stolonifera in vivo and in vitro.
abstract: Ipomoea stolonifera (I. stolonifera) has been used for the treatment of inflammatory diseases including rheumatism and rheumatoid arthritis in Chinese traditional medicine. However, the anti-inflammatory activity of I. stolonifera has not been elucidated. For this reason, the anti-inflammatory activity of n-butanol extract of I. stolonifera (BE-IS) was evaluated in vivo by using acute models (croton oil-induced mouse ear edema, carrageenan-induced rat paw edema, and carrageenan-induced rat pleurisy) and chronic models (cotton pellet-induced rat granuloma, and complete Freund's adjuvant (CFA)-induced rat arthritis). Results indicated that oral administration of BE-IS significantly attenuated croton oil-induced ear edema, decreased carrageenan-induced paw edema, reduced carrageenan-induced exudates and cellular migration, inhibited cotton pellet-induced granuloma formation and improved CFA-induced arthritis. Preliminary mechanism studies demonstrated that BE-IS decreased the levels of myeloperoxidase (MPO) and malondialdehyde (MDA), increased the activity of anti-oxidant enzyme superoxide dismutase (SOD) in vivo, and reduced the production of nitric oxide (NO), prostaglandin E2 (PGE2), tumor necrosis factor-α (TNF-α), interleukin (IL)-1β and IL-6 in lipopolysaccharide-activated RAW264.7 macrophages in vitro. Results obtained in vivo and in vitro demonstrate that BE-IS has considerable anti-inflammatory potential, which provided experimental evidences for the traditional application of Ipomoea stolonifera in inflammatory diseases.
authors.affiliation: Department of Pharmacology, Shantou University Medical College, Shantou, China; Department of Pharmacy, Second Affiliated Hospital, Shantou University Medical College, Shantou, China.
authors.forename: Congyi
authors.initials: C
authors.lastname: Cai
authors.affiliation: Department of Pharmacology, Shantou University Medical College, Shantou, China.
authors.forename: Yicun
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Biochemistry and Molecular Biology, Keck School of Medicine, University of Southern California, Los Angeles, California, United States of America.
authors.forename: Shuping
authors.initials: S
authors.lastname: Zhong
authors.affiliation: Department of Pharmacology, Shantou University Medical College, Shantou, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Ji
authors.affiliation: Department of Chemistry, Shantou University Medical College, Shantou, China.
authors.forename: Jinzhi
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Pharmacology, Shantou University Medical College, Shantou, China.
authors.forename: Xueting
authors.initials: X
authors.lastname: Bai
authors.affiliation: Department of Pharmacology, Shantou University Medical College, Shantou, China; Department of Cardiovascular Diseases, First Affiliated Hospital, Shantou University Medical College, Shantou, China.
authors.forename: Ganggang
authors.initials: G
authors.lastname: Shi
mesh_terms: D020001:1-Butanol / Q000737:chemistry*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use*; D002351:Carrageenan / Q000633:toxicity; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy; D027741:Ipomoea / Q000737:chemistry*; D008297:Male; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*
chemical_list: D000893:Anti-Inflammatory Agents; D010936:Plant Extracts; D020001:1-Butanol; D002351:Carrageenan
references.citation: Drugs. 1996;52 Suppl 2:9-17
references.pmid: 8869831
references.citation: J Ethnopharmacol. 2010 Sep 15;131(2):485-96
references.pmid: 20643199
references.citation: Proc Soc Exp Biol Med. 1962 Dec;111:544-7
references.pmid: 14001233
references.citation: J Trauma. 1995 Sep;39(3):404-10
references.pmid: 7473900
references.citation: Carcinogenesis. 2003 Feb;24(2):235-41
references.pmid: 12584172
references.citation: J Environ Biol. 2005 Jun;26(2 Suppl):383-6
references.pmid: 16334271
references.citation: Science. 1977 Sep 9;197(4308):1071-2
references.pmid: 17836071
references.citation: Br J Surg. 1993 Mar;80(3):289-97
references.pmid: 8472134
references.citation: Clin Rev Allergy Immunol. 2003 Apr;24(2):189-98
references.pmid: 12668898
references.citation: J Invest Dermatol. 1982 Mar;78(3):206-9
references.pmid: 6276474
references.citation: Int Immunopharmacol. 2013 Feb;15(2):296-302
references.pmid: 23270759
references.citation: Annu Rev Immunol. 1989;7:625-55
references.pmid: 2540776
references.citation: Mar Drugs. 2013 Aug 12;11(8):2846-72
references.pmid: 23941823
references.citation: Annu Rev Pharmacol Toxicol. 1998;38:97-120
references.pmid: 9597150
references.citation: Fed Proc. 1982 Jul;41(9):2588-95
references.pmid: 6806127
references.citation: Pharmazie. 1999 Jun;54(6):464-6
references.pmid: 10399194
references.citation: Nihon Yakurigaku Zasshi. 1992 May;99(5):363-72
references.pmid: 1592319
references.citation: J Am Med Assoc. 1953 Jul 25;152(13):1207-13
references.pmid: 13061241
references.citation: Eur J Pharmacol. 1983 Nov 11;95(1-2):1-12
references.pmid: 6583058
references.citation: Drugs. 2012 May 7;72(7):873-9
references.pmid: 22564130
references.citation: Fitoterapia. 2004 Mar;75(2):123-9
references.pmid: 15030915
references.citation: Chem Immunol. 2000;74:141-61
references.pmid: 10608086
references.citation: Agents Actions. 1989 Mar;26(3-4):335-41
references.pmid: 2567568
references.citation: Am J Bot. 2005 Sep;92(9):1513-9
references.pmid: 21646169
references.citation: J Pharm Pharmacol. 2011 Aug;63(8):1070-7
references.pmid: 21718291
references.citation: Arzneimittelforschung. 1984;34(5):599-603
references.pmid: 6540578
references.citation: Br J Pharmacol. 1993 Oct;110(2):701-6
references.pmid: 8242242
references.citation: J Immunol Methods. 1983 Dec 16;65(1-2):55-63
references.pmid: 6606682
references.citation: Nature. 2008 Jul 24;454(7203):428-35
references.pmid: 18650913
references.citation: Agents Actions. 1986 Dec;19(5-6):371-3
references.pmid: 3825756
references.citation: Annu Rev Immunol. 1990;8:253-78
references.pmid: 2188664
references.citation: J Ethnopharmacol. 2011 May 17;135(2):545-52
references.pmid: 21466841
references.citation: Asian Pac J Trop Biomed. 2012 May;2(5):364-7
references.pmid: 23569931
references.citation: J Am Pharm Assoc Am Pharm Assoc. 1957 Sep;46(9):515-9
references.pmid: 13491439
references.citation: Nature. 2008 Jul 24;454(7203):436-44
references.pmid: 18650914
references.citation: Curr Med Chem. 2004 Jul;11(13):1671-92
references.pmid: 15279576
references.citation: Agents Actions. 1986 Jan;17(3-4):347-9
references.pmid: 3962781
references.citation: Pharmacol Res. 2006 Mar;53(3):233-40
references.pmid: 16406805
references.citation: Planta Med. 1991 Dec;57(6):515-8
references.pmid: 1818340
references.citation: Curr Protein Pept Sci. 2013 May;14(3):199-204
references.pmid: 23721316
references.citation: J Bacteriol. 1993 Sep;175(18):5745-53
references.pmid: 8376321
references.citation: Evid Based Complement Alternat Med. 2012;2012:142087
references.pmid: 22899950
references.citation: J Ethnopharmacol. 2000 Jan;69(1):85-90
references.pmid: 10661888
references.citation: Evid Based Complement Alternat Med. 2011;2011:908059
references.pmid: 21785638
references.citation: Fundam Clin Pharmacol. 2007 Apr;21(2):157-64
references.pmid: 17391288
references.citation: Curr Med Chem. 2010;17(12):1191-219
references.pmid: 20158472
references.citation: Mol Immunol. 1998 Oct;35(14-15):977-87
references.pmid: 9881693
references.citation: Environ Manage. 2010 Nov;46(5):738-47
references.pmid: 20859628