title: A novel allele of the P-starvation tolerance gene OsPSTOL1 from African rice (Oryza glaberrima Steud) and its distribution in the genus Oryza.
abstract: We have developed allele-specific markers for molecular breeding to transfer the PSTOL1 gene from Kasalath to African mega-varieties, including NERICAs, to improve their tolerance to P-deficient soil. The deficiency of phosphorus (P) in soil is a major problem in Sub-Saharan Africa due to general nutrient depletion and the presence of P-fixing soils. Developing rice cultivars with enhanced P efficiency would, therefore, represent a sustainable strategy to improve the livelihood of resource-poor farmers. Recently the Pup1 locus, a major QTL for tolerance to P deficiency in soil, was successfully narrowed-down to a major gene, the protein kinase OsPSTOL1 (P-starvation tolerance), which was found to be generally absent from modern irrigated rice varieties. Our target is to improve the tolerance of African mega-varieties to P deficiency through marker-assisted introgression of PSTOL1. As a first step, we have determined the Pup1 haplotype and surveyed the presence or absence of PSTOL1 and other genes of the Pup1 locus in African mega-varieties, NERICAs (New Rice for Africa) and their Oryza glaberrima parents. Here, we report the presence of a novel PSTOL1 allele in upland NERICAs that was inherited from the O. glaberrima parent CG14. This allele showed a 35 base-pair substitution when aligned to the Kasalath allele, but maintained a fully conserved kinase domain, and is present in most O. glaberrima accessions evaluated. In-silico and marker analysis indicated that many other genes of the Kasalath Pup1 locus were missing in the O. glaberrima genome, including the dirigent-like gene OsPupK20-2, which was shown to be downstream of PSTOL1. We have developed several allele-specific markers for the use for molecular breeding to transfer the PSTOL1 gene from Kasalath to African mega-varieties, including NERICAs.
authors.affiliation: Japan International Research Center for Agricultural Sciences (JIRCAS), 1-1 Ohwashi, Tsukuba, Ibaraki, 305-8686, Japan.
authors.forename: Juan
authors.initials: J
authors.lastname: Pariasca-Tanaka
authors.forename: Joong Hyoun
authors.initials: JH
authors.lastname: Chin
authors.forename: Khady Nani
authors.initials: KN
authors.lastname: Dramé
authors.forename: Cheryl
authors.initials: C
authors.lastname: Dalid
authors.forename: Sigrid
authors.initials: S
authors.lastname: Heuer
authors.forename: Matthias
authors.initials: M
authors.lastname: Wissuwa
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D017343:Genes, Plant; D005838:Genotype; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics* / Q000378:metabolism; D010758:Phosphorus / Q000378:metabolism*; D040641:Quantitative Trait Loci; D012333:RNA, Messenger / Q000378:metabolism; D016415:Sequence Alignment; D012987:Soil / Q000737:chemistry; D013312:Stress, Physiological / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D012987:Soil; D010758:Phosphorus
references.citation: Plant Physiol. 2011 Jul;156(3):1202-16
references.pmid: 21602323
references.citation: Theor Appl Genet. 2010 Apr;120(6):1073-86
references.pmid: 20035315
references.citation: Theor Appl Genet. 2002 Nov;105(6-7):890-897
references.pmid: 12582914
references.citation: Plant Biotechnol J. 2009 Jun;7(5):456-7
references.pmid: 19422603
references.citation: Nature. 2012 Aug 23;488(7412):535-9
references.pmid: 22914168
references.citation: Science. 2002 Mar 15;295(5562):2019-20
references.pmid: 11896257