title: Correlated miR-mRNA expression signatures of mouse hematopoietic stem and progenitor cell subsets predict "Stemness" and "Myeloid" interaction networks.
abstract: Several individual miRNAs (miRs) have been implicated as potent regulators of important processes during normal and malignant hematopoiesis. In addition, many miRs have been shown to fine-tune intricate molecular networks, in concert with other regulatory elements. In order to study hematopoietic networks as a whole, we first created a map of global miR expression during early murine hematopoiesis. Next, we determined the copy number per cell for each miR in each of the examined stem and progenitor cell types. As data is emerging indicating that miRs function robustly mainly when they are expressed above a certain threshold (∼100 copies per cell), our database provides a resource for determining which miRs are expressed at a potentially functional level in each cell type. Finally, we combine our miR expression map with matched mRNA expression data and external prediction algorithms, using a Bayesian modeling approach to create a global landscape of predicted miR-mRNA interactions within each of these hematopoietic stem and progenitor cell subsets. This approach implicates several interaction networks comprising a "stemness" signature in the most primitive hematopoietic stem cell (HSC) populations, as well as "myeloid" patterns associated with two branches of myeloid development.
authors.affiliation: Center for Stem Cell Biology and Regenerative Medicine, Departments of Pediatrics and Physiology, University of Maryland School of Medicine, Baltimore, Maryland, United States of America; Department of Oncology, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America.
authors.forename: Diane
authors.initials: D
authors.lastname: Heiser
authors.affiliation: Center for Stem Cell Biology and Regenerative Medicine, Departments of Pediatrics and Physiology, University of Maryland School of Medicine, Baltimore, Maryland, United States of America.
authors.forename: Yee Sun
authors.initials: YS
authors.lastname: Tan
authors.affiliation: Center for Stem Cell Biology and Regenerative Medicine, Departments of Pediatrics and Physiology, University of Maryland School of Medicine, Baltimore, Maryland, United States of America.
authors.forename: Ian
authors.initials: I
authors.lastname: Kaplan
authors.affiliation: Center for Stem Cell Biology and Regenerative Medicine, Departments of Pediatrics and Physiology, University of Maryland School of Medicine, Baltimore, Maryland, United States of America.
authors.forename: Brian
authors.initials: B
authors.lastname: Godsey
authors.affiliation: Center for Stem Cell Biology and Regenerative Medicine, Departments of Pediatrics and Physiology, University of Maryland School of Medicine, Baltimore, Maryland, United States of America.
authors.forename: Sebastien
authors.initials: S
authors.lastname: Morisot
authors.affiliation: Center for Stem Cell Biology and Regenerative Medicine, Departments of Pediatrics and Physiology, University of Maryland School of Medicine, Baltimore, Maryland, United States of America.
authors.forename: Wen-Chih
authors.initials: WC
authors.lastname: Cheng
authors.affiliation: Department of Oncology, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America.
authors.forename: Donald
authors.initials: D
authors.lastname: Small
authors.affiliation: Center for Stem Cell Biology and Regenerative Medicine, Departments of Pediatrics and Physiology, University of Maryland School of Medicine, Baltimore, Maryland, United States of America.
authors.forename: Curt I
authors.initials: CI
authors.lastname: Civin
mesh_terms: D000465:Algorithms; D000818:Animals; D002454:Cell Differentiation; D005260:Female; D020869:Gene Expression Profiling; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D023461:Myeloid Progenitor Cells / Q000166:cytology* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D035683:MicroRNAs; D012333:RNA, Messenger
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