title: Effect of Aloe vera (Aloe barbadensis Miller) on survivability, extent of proteolysis and ACE inhibition of potential probiotic cultures in fermented milk.
abstract: BACKGROUND
  In the present investigation, the effect of Aloe vera gel powder on angiotensin-converting enzyme (ACE) inhibitory activity, extent of proteolysis during fermentation and survival of Lactobacillus casei NCDC19 during storage of fermented milk was studied.
  
  
  RESULTS
  Among the different cultures screened for ACE inhibitory activity, Lactobacillus casei NCDC 19 exhibited the highest ACE inhibition (approx. 40%) as well as extent of proteolysis (0.37, Abs₃₄₀). In the presence of Aloe vera (0.5% and 1% w/v) an increase in extent of proteolysis (0.460 ± 0.047 and 0.480 ± 0.027) and percent ACE inhibitory activity (44.32 ± 2.83 and 47.52 ± 1.83) was observed in comparison to control. Aloe vera powder addition also led to an increase in viable counts (>11 log cfu mL⁻¹) of L. casei NCDC 19 in fermented milk during storage for 7 days and the counts were maintained in sufficiently higher numbers.
  
  
  CONCLUSIONS
  The study suggests Aloe vera to be a good functional ingredient which can be further explored for different health attributes.
authors.affiliation: Division of Animal Biochemistry, National Dairy Research Institute, Karnal, -132001, Haryana, India.
authors.forename: Santosh
authors.initials: S
authors.lastname: Basannavar
authors.forename: Ramesh
authors.initials: R
authors.lastname: Pothuraju
authors.forename: Raj Kumar
authors.initials: RK
authors.lastname: Sharma
mesh_terms: D000504:Aloe / Q000737:chemistry*; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000737:chemistry*; D015169:Colony Count, Microbial; D043302:Cultured Milk Products / Q000737:chemistry / Q000378:metabolism / Q000382:microbiology*; D018752:Diet, Fat-Restricted; D005285:Fermentation; D005503:Food Additives / Q000737:chemistry*; D061353:Food Storage; D005782:Gels; D006863:Hydrogen-Ion Concentration; D007194:India; D007779:Lactobacillus acidophilus / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism; D007780:Lacticaseibacillus casei / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism; D050296:Microbial Viability; D028321:Plant Preparations / Q000737:chemistry*; D011208:Powders; D019936:Probiotics / Q000378:metabolism; D059748:Proteolysis; D012034:Refrigeration
chemical_list: C586178:Aloe vera gel; D000806:Angiotensin-Converting Enzyme Inhibitors; D005503:Food Additives; D005782:Gels; D028321:Plant Preparations; D011208:Powders
keywords: Aloe vera; Lactobacillus casei; angiotensin-converting enzyme; proteolysis; viability