title: MSX ₁ gene variant and non-syndromic clefting: association or rejection?
abstract: BACKGROUND
  Non-syndromic cleft lip/palate (NSCL/P) is a congenital anomaly with significant medical, psychological and social ramifications. There is sufficient evidence to hypothesize that locus for this condition can be identified by candidate genes.
  
  
  OBJECTIVE
  The aim of this study is to amplify the chosen region (799 G >T) of MSX 1 gene, investigate the degree of association and perform a mutation research from Raichur cleft lip and palate patient sample.
  
  
  METHODS
  Case history and clinical examination of the patient were recorded to rule. Written consent was obtained from patients and controls for in vivo study. STUDY WAS DESIGNED IN FOUR STEPS AS FOLLOWS: a. Collection of a blood sample; b. Genomic deoxyribonucleic acid (DNA) extraction; c. Polymerase chain reaction (PCR); d. Restriction fragment length polymorphism (RFLP).
  
  
  METHODS
  Blood samples were collected from 50 subjects having NSCL/P and 50 controls. Genomic DNA was extracted, PCR and RFLP was performed for digestion products that were evaluated.
  
  
  METHODS
  Chi-square test with P value at 95% confidence intervals.
  
  
  RESULTS
  The results showed a positive correlation between MSX 1 799 G >T gene variant and NSCL/P patients in Raichur patients.
  
  
  CONCLUSIONS
  From a genetically diverse etiology MSX 1 799 G >T gene variant may be a good screening marker for NSCL/P in Raichur patients.
authors.forename: Naveen Admala
authors.initials: NA
authors.lastname: Reddy
authors.forename: Adusumilli
authors.initials: A
authors.lastname: Gopinath
authors.forename: Jayaprakash Thirumala
authors.initials: JT
authors.lastname: Reddy
authors.forename: Raghu
authors.initials: R
authors.lastname: Devanna
authors.forename: Pichai
authors.initials: P
authors.lastname: Saravanan
authors.affiliation: Departments of Orthodontics, and Dentofacial Orthopaedics, AME's Dental College, Hospital and Research Center, Raichur, Karnataka, India.
authors.forename: Mayur G
authors.initials: MG
authors.lastname: Rohra
mesh_terms: D002971:Cleft Lip / Q000235:genetics*; D002972:Cleft Palate / Q000235:genetics*; D006801:Humans; D051957:MSX1 Transcription Factor / Q000235:genetics*; D016133:Polymerase Chain Reaction
chemical_list: D051957:MSX1 Transcription Factor; C484083:MSX1 protein, human