title: DNA methylation study of fetus genome through a genome-wide analysis.
abstract: BACKGROUND
  DNA methylation is a crucial epigenetic modification of the genome which is involved in embryonic development, transcription, chromatin structure, X chromosome inactivation, genomic imprinting and chromosome stability. Consistent with these important roles, DNA methylation has been demonstrated to be required for vertebrate early embryogenesis and essential for regulating temporal and spatial expression of genes controlling cell fate and differentiation. Further studies have shown that abnormal DNA methylation is associated with human diseases including the embryonic development diseases. We attempt to study the DNA methylation status of CpG islands in fetus related to fetus growth and development.
  
  
  METHODS
  GeneChip® Human Tiling 2.0R Array set is used for analysis of methylated DNA in a whole-genome wide in 8 pairs amniotic fluid and maternal blood DNA samples.
  
  
  RESULTS
  We found 1 fetus hypermethylation DNA markers and 4 fetus hypomethylation DNA markers though a Genome-wide analysis. These DNA markers all found to be associated with the critical genes for fetus growth and development (SH2D3C gene, EML3 gene, TRIM71 gene, HOXA3 gene and HOXA5 gene).
  
  
  CONCLUSIONS
  These genes can be used as a biomarker for association studying of embryonic development, pathological pregnancy and so on. The present study has provided new and fundamental insights into the roles that DNA methylation has in embryonic development and in the pathological pregnancy.
authors.forename: Hong-Dan
authors.initials: HD
authors.lastname: Wang
authors.forename: Qiao-Fang
authors.initials: QF
authors.lastname: Hou
authors.forename: Qian-Nan
authors.initials: QN
authors.lastname: Guo
authors.forename: Tao
authors.initials: T
authors.lastname: Li
authors.forename: Dong
authors.initials: D
authors.lastname: Wu
authors.forename: Xian-Ping
authors.initials: XP
authors.lastname: Zhang
authors.forename: Yan
authors.initials: Y
authors.lastname: Chu
authors.forename: Miao
authors.initials: M
authors.lastname: He
authors.forename: Hai
authors.initials: H
authors.lastname: Xiao
authors.forename: Liang-Jie
authors.initials: LJ
authors.lastname: Guo
authors.forename: Ke
authors.initials: K
authors.lastname: Yang
authors.affiliation: Medical Genetic Institute of Henan Province, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, Zhengzhou 450003, PR China. syyliaoshixiu@163.com.
authors.forename: Shi-Xiu
authors.initials: SX
authors.lastname: Liao
authors.forename: Bo-Feng
authors.initials: BF
authors.lastname: Zhu
mesh_terms: D000328:Adult; D002891:Chromosomes, Human, Pair 21 / Q000235:genetics; D019175:DNA Methylation / Q000235:genetics*; D005260:Female; D005333:Fetus / Q000378:metabolism*; D005819:Genetic Markers; D015894:Genome, Human / Q000235:genetics*; D006801:Humans; D011247:Pregnancy; D060888:Real-Time Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012984:Software
chemical_list: D005819:Genetic Markers
references.citation: Cancer Epidemiol Biomarkers Prev. 2014 Feb;23(2):223-33
references.pmid: 24326628
references.citation: Comp Biochem Physiol B Biochem Mol Biol. 2013 Sep;166(1):99-108
references.pmid: 23876386
references.citation: Int J Biol Sci. 2013 Dec 09;9(10):1134-44
references.pmid: 24339734
references.citation: Mol Biol Rep. 2009 Feb;36(2):227-35
references.pmid: 17972163
references.citation: Nat Biotechnol. 2010 May;28(5):516-20
references.pmid: 20436463
references.citation: Curr Mol Med. 2014 Jan;14(1):45-57
references.pmid: 24295492
references.citation: Okajimas Folia Anat Jpn. 1999 May;76(1):47-53
references.pmid: 10409845
references.citation: Hum Mol Genet. 2014 Mar 1;23(5):1224-36
references.pmid: 24186870
references.citation: Mol Cell. 2011 Oct 7;44(1):17-28
references.pmid: 21924933
references.citation: J Bone Miner Res. 2009 Dec;24(12):2039-49
references.pmid: 19453261
references.citation: Nat Genet. 2010 Dec;42(12):1093-100
references.pmid: 21057502
references.citation: J Cell Sci. 2008 May 15;121(Pt 10):1718-26
references.pmid: 18445686
references.citation: Genome Res. 2014 Jan;24(1):70-83
references.pmid: 24065774
references.citation: Nature. 2011 Sep 04;477(7366):606-10
references.pmid: 21892189
references.citation: PLoS One. 2013 Dec 02;8(12):e82302
references.pmid: 24312652
references.citation: Mol Reprod Dev. 2010 Feb;77(2):105-13
references.pmid: 19921744
references.citation: Mol Cell Biol. 2006 Oct;26(19):7077-85
references.pmid: 16980612
references.citation: Science. 2001 Aug 10;293(5532):1068-70
references.pmid: 11498573
references.citation: Br J Cancer. 2013 Dec 10;109(12):3132
references.pmid: 24327071
references.citation: Clin Chem Lab Med. 2014 May;52(5):735-42
references.pmid: 24327526
references.citation: Nature. 2010 Jan 28;463(7280):563-7
references.pmid: 20081831
references.citation: Nat Commun. 2012 Jun 26;3:923
references.pmid: 22735451
references.citation: Cell Stem Cell. 2009 Oct 2;5(4):442-9
references.pmid: 19796624
references.citation: Nat Commun. 2013;4:2889
references.pmid: 24326934
references.citation: Mol Biol Evol. 2007 Nov;24(11):2525-34
references.pmid: 17890240
references.citation: Epigenomics. 2013 Dec;5(6):715-28
references.pmid: 24283884