title: Identification and characterization of ABA receptors in Oryza sativa.
abstract: Abscisic acid (ABA) is an essential phytohormone that regulates plant stress responses. ABA receptors in Arabidopsis thaliana (AtPYLs) have been extensively investigated by structural, biochemical, and in vivo studies. In contrast, relatively little is known about the ABA signal transduction cascade in rice. Besides, the diversities of AtPYLs manifest that the information accumulated in Arabidopsis cannot be simply adapted to rice. Thus, studies on rice ABA receptors are compulsory. By taking a bioinformatic approach, we identified twelve ABA receptor orthologs in Oryza sativa (japonica cultivar-group) (OsPYLs), named OsPYL1-12. We have successfully expressed and purified OsPYL1-3, 6 and 10-12 to homogeneity, tested the inhibitory effects on PP2C in Oryza sativa (OsPP2C), and measured their oligomerization states. OsPYL1-3 mainly exhibit as dimers and require ABA to inhibit PP2C's activity. On the contrary, OsPYL6 retains in the monomer-dimer equilibrium state and OsPYL10-11 largely exist as monomers, and they all display an ABA-independent phosphatase inhibition manner. Interestingly, although OsPYL12 seems to be a dimer, it abrogates the phosphatase activity of PP2Cs in the absence of ABA. Toward a further understanding of OsPYLs on the ABA binding and PP2C inhibition, we determined the crystal structure of ABA-OsPYL2-OsPP2C06 complex. The bioinformatic, biochemical and structural analysis of ABA receptors in rice provide important foundations for designing rational ABA-analogues and breeding the stress-resistant rice for commercial agriculture.
authors.affiliation: State Key Laboratory of Bio-membrane and Membrane Biotechnology, Beijing, China; Center for Structural Biology, School of Medicine, Beijing, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: He
authors.affiliation: State Key Laboratory of Bio-membrane and Membrane Biotechnology, Beijing, China; Center for Structural Biology, School of Medicine, Beijing, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Hao
authors.affiliation: State Key Laboratory of Bio-membrane and Membrane Biotechnology, Beijing, China; Center for Structural Biology, School of Medicine, Beijing, China.
authors.forename: Wenqi
authors.initials: W
authors.lastname: Li
authors.affiliation: State Key Laboratory of Bio-membrane and Membrane Biotechnology, Beijing, China; School of Life Sciences, Tsinghua University, Beijing, China.
authors.forename: Chuangye
authors.initials: C
authors.lastname: Yan
authors.affiliation: State Key Laboratory of Bio-membrane and Membrane Biotechnology, Beijing, China; Center for Structural Biology, School of Medicine, Beijing, China.
authors.forename: Nieng
authors.initials: N
authors.lastname: Yan
authors.affiliation: National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Wuhan, China; College of Life Sciences and Technology, Huazhong Agricultural University, Wuhan, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Yin
mesh_terms: D000040:Abscisic Acid / Q000235:genetics / Q000378:metabolism; D012275:Oryza / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D010749:Phosphoprotein Phosphatases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D055503:Protein Multimerization / Q000502:physiology; D000071636:Protein Phosphatase 2C; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology
chemical_list: D010940:Plant Proteins; D011956:Receptors, Cell Surface; D000040:Abscisic Acid; D010749:Phosphoprotein Phosphatases; D000071636:Protein Phosphatase 2C
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