title: The effect of oxidized cholesterol on barrier functions and IL-10 mRNA expression in human intestinal epithelium co-cultured with dendritic cells in the transwell system.
abstract: The intestinal epithelium is exposed to oxygenated cholesterol products present in foodstuffs. In vitro studies demonstrate the effect of oxysterols on cytokine release by intestinal cells cultured alone. However, physiologically, the response of the intestinal epithelium to external agents occurs in the presence of dendritic cells (DCs). The aim of the study was to analyze the effect of 7-ketocholesterol on the barrier functions and IL-10 mRNA expression of Caco-2 cells in the presence of DCs, and secondly, on IL-10 mRNA expression in DCs. Caco-2 cells were co-cultured with monocyte-derived dendritic cells and induced with 7-ketocholesterol in a transwell system. DCs did not affect the transepithelial electrical resistance (TER) of the Caco-2 cell monolayer, but increased IL-10 mRNA expression in Caco-2 cells. 7-ketocholesterol decreased the TER of Caco-2 cells co-cultured with DCs and diminished IL-10 mRNA expression in Caco-2 cells induced by the presence of DCs. IL-10 mRNA expression fell in DCs co-cultured with Caco-2 cells after treatment with 7-ketocholesterol. Oxidized cholesterols present in gut mucosa may contribute to the decrease of epithelial barrier functions and the inappropriate development of an inflammatory response to food compounds.
authors.affiliation: Dept. of Internal Diseases and Clinical Pharmacology, Laboratory of Tissue Immunopharmacology, Medical University of Lodz, Kniaziewicza 1/5, 91-347 Lodz, Poland. Electronic address: maciej.chalubinski@umed.lodz.pl.
authors.forename: Maciej
authors.initials: M
authors.lastname: Chalubinski
authors.affiliation: Dept. of Internal Diseases and Clinical Pharmacology, Laboratory of Tissue Immunopharmacology, Medical University of Lodz, Kniaziewicza 1/5, 91-347 Lodz, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Wojdan
authors.affiliation: Dept. of Internal Diseases and Clinical Pharmacology, Laboratory of Tissue Immunopharmacology, Medical University of Lodz, Kniaziewicza 1/5, 91-347 Lodz, Poland.
authors.forename: Paulina
authors.initials: P
authors.lastname: Gorzelak
authors.affiliation: Immunopathology and Genetics Laboratory, Department of Pediatrics, Oncology, Hematology and Diabetology, Medical University of Lodz, Sporna 36/50, 91-738 Lodz, Poland; Department of Clinical Genetics, Medical University of Lodz, Sterlinga 1/3, 90-425 Lodz, Poland.
authors.forename: Maciej
authors.initials: M
authors.lastname: Borowiec
authors.affiliation: Dept. of Internal Diseases and Clinical Pharmacology, Laboratory of Tissue Immunopharmacology, Medical University of Lodz, Kniaziewicza 1/5, 91-347 Lodz, Poland.
authors.forename: Marlena
authors.initials: M
authors.lastname: Broncel
mesh_terms: D018938:Caco-2 Cells / Q000187:drug effects; D018920:Coculture Techniques / Q000295:instrumentation / Q000379:methods; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects*; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics*; D007413:Intestinal Mucosa / Q000166:cytology / Q000187:drug effects*; D007653:Ketocholesterols / Q000494:pharmacology*; D062793:Occludin / Q000235:genetics; D012333:RNA, Messenger; D062826:Zonula Occludens-1 Protein / Q000235:genetics
chemical_list: C508609:IL10 protein, human; D007653:Ketocholesterols; C568985:OCLN protein, human; D062793:Occludin; D012333:RNA, Messenger; C569042:TJP1 protein, human; D062826:Zonula Occludens-1 Protein; D016753:Interleukin-10; C003001:7-ketocholesterol
keywords: 7-Ketocholesterol; Dendritic cells; Epithelial barrier; IL-10; Tight junctions