title: Nanostructured weathering steel for matrix-free laser desorption ionisation mass spectrometry and imaging of metabolites, drugs and complex glycans.
abstract: Weathering steel has been employed for the first time to prepare sample plates for matrix-free laser desorption ionisation mass spectrometry (LDI-MS) of small molecules up to a mass range of around 1500 Da. The effective UV absorption, heat conductivity and porosity of the nanostructured inner rust layer formed during passivation determine the excellent performance in LDI-MS for a broad range of different analyte classes. The inexpensive material was evaluated in a series of relevant analytical applications ranging from the matrix-free detection of serum metabolites, lactose quantification, lipid analysis in milk to the glycoprofiling of antibodies and imaging mass spectrometry of brain tissue samples.
authors.affiliation: Biofunctional Nanomaterials Unit, CICbiomaGUNE, Paseo Miramón 182, 20009 San Sebastian, Spain. nreichardt@cicbiomagune.es.
authors.forename: Juan
authors.initials: J
authors.lastname: Etxebarria
authors.forename: Javier
authors.initials: J
authors.lastname: Calvo
authors.forename: Niels-Christian
authors.initials: NC
authors.lastname: Reichardt
mesh_terms: D006801:Humans; D049329:Nanostructures; D004364:Pharmaceutical Preparations / Q000032:analysis* / Q000097:blood; D011134:Polysaccharides / Q000032:analysis* / Q000097:blood; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet; D013232:Steel
chemical_list: D004364:Pharmaceutical Preparations; D011134:Polysaccharides; D013232:Steel