title: Luminol-based chemiluminescent signals: clinical and non-clinical application and future uses.
abstract: Chemiluminescence (CL) is an important method for quantification and analysis of various macromolecules. A wide range of CL agents such as luminol, hydrogen peroxide, fluorescein, dioxetanes and derivatives of oxalate, and acridinium dyes are used according to their biological specificity and utility. This review describes the application of luminol chemiluminescence (LCL) in forensic, biomedical, and clinical sciences. LCL is a very useful detection method due to its selectivity, simplicity, low cost, and high sensitivity. LCL has a dynamic range of applications, including quantification and detection of macro and micromolecules such as proteins, carbohydrates, DNA, and RNA. Luminol-based methods are used in environmental monitoring as biosensors, in the pharmaceutical industry for cellular localization and as biological tracers, and in reporter gene-based assays and several other immunoassays. Here, we also provide information about different compounds that may enhance or inhibit the LCL along with the effect of pH and concentration on LCL. This review covers most of the significant information related to the applications of luminol in different fields.
authors.affiliation: Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, Jamia Nagar, New Delhi, 110025, India.
authors.forename: Parvez
authors.initials: P
authors.lastname: Khan
authors.forename: Danish
authors.initials: D
authors.lastname: Idrees
authors.forename: Michael A
authors.initials: MA
authors.lastname: Moxley
authors.forename: John A
authors.initials: JA
authors.lastname: Corbett
authors.forename: Faizan
authors.initials: F
authors.lastname: Ahmad
authors.forename: Guido
authors.initials: G
authors.lastname: von Figura
authors.forename: William S
authors.initials: WS
authors.lastname: Sly
authors.forename: Abdul
authors.initials: A
authors.lastname: Waheed
authors.forename: Md Imtaiyaz
authors.initials: MI
authors.lastname: Hassan
mesh_terms: D000818:Animals; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008163:Luminescent Measurements / Q000379:methods*; D008165:Luminol / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry
chemical_list: D011506:Proteins; D008165:Luminol
references.citation: Genome Res. 2001 Apr;11(4):600-8
references.pmid: 11282975
references.citation: PLoS One. 2012;7(7):e40879
references.pmid: 22911714
references.citation: J Forensic Sci. 1978 Jul;23(3):550-62
references.pmid: 744985
references.citation: Methods Enzymol. 1986;133:493-507
references.pmid: 3821550
references.citation: Talanta. 2007 May 15;72(3):896-913
references.pmid: 19071703
references.citation: J Immunol Methods. 2001 Sep 1;255(1-2):1-13
references.pmid: 11470281
references.citation: Appl Biochem Biotechnol. 2014 Feb;172(3):1320-31
references.pmid: 24166104
references.citation: Clin Chem. 1996 Sep;42(9):1518-26
references.pmid: 8787723
references.citation: Luminescence. 2011 Nov-Dec;26(6):397-402
references.pmid: 22218870
references.citation: Anal Chem. 2012 Nov 20;84(22):9951-5
references.pmid: 23101695
references.citation: Biochim Biophys Acta. 1995 May 17;1256(2):233-40
references.pmid: 7766702
references.citation: Int J Oncol. 2003 Feb;22(2):339-43
references.pmid: 12527932
references.citation: J AOAC Int. 2011 Sep-Oct;94(5):1461-6
references.pmid: 22165010
references.citation: Methods Mol Biol. 2013;919:79-85
references.pmid: 22976092
references.citation: J Nutr. 2001 Nov;131(11):2916-20
references.pmid: 11694618
references.citation: Chemistry. 2012 Dec 7;18(50):16030-6
references.pmid: 23081856
references.citation: Am J Trop Med Hyg. 2002 Dec;67(6):632-5
references.pmid: 12518854
references.citation: J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Sep 15;857(1):158-63
references.pmid: 17627906
references.citation: Angew Chem Int Ed Engl. 2003 May 30;42(21):2372-6
references.pmid: 12783499
references.citation: Talanta. 2012 Aug 15;97:193-8
references.pmid: 22841066
references.citation: Biosens Bioelectron. 2013 May 15;43:1-5
references.pmid: 23274189
references.citation: Methods Enzymol. 1977;46:216-20
references.pmid: 909409
references.citation: Anal Chem. 2001 Nov 1;73(21):5043-51
references.pmid: 11721898
references.citation: J Chromatogr A. 2006 Jun 9;1117(2):176-83
references.pmid: 16603169
references.citation: Anal Biochem. 1997 May 1;247(2):237-41
references.pmid: 9177683
references.citation: Biosens Bioelectron. 2013 Sep 15;47:356-60
references.pmid: 23608537
references.citation: Bioconjug Chem. 2009 Feb;20(2):266-73
references.pmid: 19140729
references.citation: Anal Chem. 2014 Mar 18;86(6):3092-9
references.pmid: 24552272
references.citation: J Forensic Sci. 2000 Sep;45(5):1090-2
references.pmid: 11005186
references.citation: Drugs Exp Clin Res. 2002;28(4):133-45
references.pmid: 12512231
references.citation: J Histochem Cytochem. 1990 Mar;38(3):415-9
references.pmid: 1689340
references.citation: Appl Biochem Biotechnol. 2009 Sep;158(3):582-94
references.pmid: 18841499
references.citation: Anal Chem. 2001 Dec 15;73(24):5777-83
references.pmid: 11791544
references.citation: J Pharm Biomed Anal. 1994 Apr;12(4):433-62
references.pmid: 7918762
references.citation: J Chromatogr B Analyt Technol Biomed Life Sci. 2012 Dec 12;911:1-5
references.pmid: 23217298
references.citation: Methods Enzymol. 1986;133:331-53
references.pmid: 3821542
references.citation: Zentralbl Bakteriol. 1995 Nov;283(1):90-4
references.pmid: 9810649
references.citation: J Pharm Biomed Anal. 1987;5(8):829-33
references.pmid: 16867459
references.citation: Anal Chim Acta. 2013 Feb 18;764:70-7
references.pmid: 23374217
references.citation: Luminescence. 2014 Mar;29(2):147-50
references.pmid: 23681963
references.citation: Clin Chem. 1996 Sep;42(9):1542-6
references.pmid: 8787727
references.citation: Anal Biochem. 1994 Jun;219(2):169-81
references.pmid: 8080073
references.citation: Anal Chem. 2003 Feb 1;75(3):556-62
references.pmid: 12585484
references.citation: Anal Biochem. 2013 Dec 1;443(1):22-6
references.pmid: 23973428
references.citation: Analyst. 2013 Jan 7;138(1):234-9
references.pmid: 23162809
references.citation: J Pharm Biomed Anal. 1998 Sep 1;17(6-7):985-99
references.pmid: 9884189
references.citation: Luminescence. 2011 Sep-Oct;26(5):313-8
references.pmid: 22021244
references.citation: J Forensic Sci. 2006 Mar;51(2):361-70
references.pmid: 16566772
references.citation: Ann Clin Biochem. 2002 Mar;39(Pt 2):114-29
references.pmid: 11928759
references.citation: Guang Pu Xue Yu Guang Pu Fen Xi. 2008 May;28(5):1026-9
references.pmid: 18720794
references.citation: Talanta. 2003 Apr 10;59(5):1029-38
references.pmid: 18968994
references.citation: Analyst. 2012 Sep 21;137(18):4247-53
references.pmid: 22852133
references.citation: Biomed Microdevices. 2013 Feb;15(1):195-202
references.pmid: 23053451
references.citation: Biotechniques. 2002 Jul;33(1):144-6, 148
references.pmid: 12139239
references.citation: Inflamm Res. 2002 Nov;51(11):557-62
references.pmid: 12540020
references.citation: Luminescence. 2007 Jan-Feb;22(1):53-9
references.pmid: 16874850
references.citation: J Forensic Sci. 2000 Mar;45(2):354-80
references.pmid: 10782955
references.citation: Free Radic Biol Med. 1996;20(6):785-92
references.pmid: 8728025
references.citation: Analyst. 2013 Feb 21;138(3):850-5
references.pmid: 23198282
references.citation: Luminescence. 2012 Sep-Oct;27(5):419-25
references.pmid: 23044772
references.citation: World J Surg. 2002 May;26(5):521-6
references.pmid: 12098037
references.citation: J Pharm Biomed Anal. 2007 Apr 11;43(5):1673-81
references.pmid: 17289328
references.citation: J Biol Chem. 1990 May 25;265(15):8795-801
references.pmid: 2111324
references.citation: Anal Biochem. 1985 Nov 15;151(1):205-9
references.pmid: 4091282
references.citation: Anal Biochem. 2001 Jul 1;294(1):55-62
references.pmid: 11412006
references.citation: Anal Chem. 1996 Apr 1;68(7):1254-7
references.pmid: 21619157
references.citation: J Biol Chem. 1992 Feb 15;267(5):3308-11
references.pmid: 1737787
references.citation: Talanta. 2007 Mar 30;71(5):1861-6
references.pmid: 19071534
references.citation: Appl Biochem Biotechnol. 2013 Sep;171(1):63-71
references.pmid: 23817786
references.citation: Talanta. 2013 Sep 15;113:106-12
references.pmid: 23708630
references.citation: Luminescence. 2005 Nov-Dec;20(6):411-3
references.pmid: 15966054
references.citation: Zhonghua Min Guo Wei Sheng Wu Ji Mian Yi Xue Za Zhi. 1992 May;25(2):78-90
references.pmid: 1473375
references.citation: Talanta. 2005 Aug 15;67(2):345-53
references.pmid: 18970174
references.citation: Anal Sci. 2012;28(8):795-9
references.pmid: 22878635
references.citation: Biosens Bioelectron. 2013 Sep 15;47:26-31
references.pmid: 23542066
references.citation: Carcinogenesis. 2002 Dec;23(12):2043-9
references.pmid: 12507927
references.citation: Fertil Steril. 2002 Dec;78(6):1215-24
references.pmid: 12477515
references.citation: Appl Biochem Biotechnol. 2013 Jun;170(4):972-9
references.pmid: 23636651
references.citation: Luminescence. 2013 Jul-Aug;28(4):592-6
references.pmid: 22807137
references.citation: Luminescence. 2001 Jan-Feb;16(1):1-23
references.pmid: 11180653
references.citation: Biotechniques. 2000 Mar;28(3):492-6
references.pmid: 10723562
references.citation: Nucleic Acids Symp Ser. 1991;(24):197-200
references.pmid: 1668687
references.citation: J Chromatogr B Analyt Technol Biomed Life Sci. 2006 Oct 20;843(1):20-4
references.pmid: 16765108
references.citation: Biosens Bioelectron. 2014 Jul 15;57:36-40
references.pmid: 24534578
references.citation: Anal Chem. 2001 Dec 15;73(24):5909-20
references.pmid: 11791560
references.citation: J Chromatogr A. 2002 Mar 15;950(1-2):1-19
references.pmid: 11990983
references.citation: Luminescence. 2013 Nov-Dec;28(6):847-52
references.pmid: 23097313
references.citation: Clin Chem. 1997 Dec;43(12):2345-52
references.pmid: 9439453
references.citation: Biosens Bioelectron. 2013 May 15;43:160-4
references.pmid: 23298628
references.citation: Biotechniques. 2001 Nov;31(5):1098-100, 1102-5
references.pmid: 11730016
references.citation: Biosens Bioelectron. 2013 Jan 15;39(1):261-7
references.pmid: 22917918
references.citation: Scand J Gastroenterol. 2002 Jul;37(7):765-71
references.pmid: 12190088
references.citation: Se Pu. 2004 Jul;22(4):349-53
references.pmid: 15709406
references.citation: J Chromatogr A. 2002 Apr 5;952(1-2):131-8
references.pmid: 12064525
references.citation: Bull Exp Biol Med. 2010 Aug;149(2):239-41
references.pmid: 21113500
references.citation: Analyst. 2012 Sep 21;137(18):4262-6
references.pmid: 22832507
references.citation: Lupus. 2002;11(4):240-8
references.pmid: 12043888
references.citation: Anal Chem. 2012 Nov 6;84(21):9492-7
references.pmid: 23025448
references.citation: Chem Cent J. 2009 Jul 01;3:7
references.pmid: 19570217
references.citation: Lipids. 2005 May;40(5):515-22
references.pmid: 16094862
references.citation: Anal Chem. 1993 Jul 1;65(13):1794-9
references.pmid: 8368532
references.citation: Analyst. 2013 Aug 7;138(15):4393-7
references.pmid: 23732463
references.citation: FEMS Immunol Med Microbiol. 1994 Mar;8(3):271-81
references.pmid: 8004064
references.citation: Biochem J. 1997 Jul 15;325 ( Pt 2):553-7
references.pmid: 9230140
references.citation: Chem Commun (Camb). 2010 Oct 28;46(40):7560-2
references.pmid: 20852765
references.citation: Biosens Bioelectron. 2013 Mar 15;41:348-53
references.pmid: 22981413
references.citation: Prostaglandins. 1996 Nov;52(5):385-401
references.pmid: 8948506
references.citation: Appl Biochem Biotechnol. 2004 May;117(2):93-102
references.pmid: 15159553
references.citation: J Clin Microbiol. 1994 Dec;32(12):2898-903
references.pmid: 7883875
references.citation: Can J Gastroenterol. 2003 Jan;17(1):31-6
references.pmid: 12560852
references.citation: Food Chem. 2012 Sep 15;134(2):1181-91
references.pmid: 23107746
references.citation: Luminescence. 1999 May-Jun;14(3):175-82
references.pmid: 10423579
references.citation: Chem Biol. 2012 Sep 21;19(9):1199-209
references.pmid: 22999887
references.citation: J Biolumin Chemilumin. 1989 Apr-Jun;3(2):59-65
references.pmid: 2728913
references.citation: Analyst. 2013 Apr 21;138(8):2269-78
references.pmid: 23443524
references.citation: Anal Chim Acta. 2012 Oct 1;745:137-42
references.pmid: 22938618
references.citation: J Virol Methods. 1996 May;59(1-2):1-11
references.pmid: 8793825
references.citation: J Pharm Biomed Anal. 1998 Dec;18(4-5):555-64
references.pmid: 9919955
references.citation: Appl Biochem Biotechnol. 2012 Feb;166(3):786-95
references.pmid: 22139733
references.citation: Biotechniques. 2001 Oct;31(4):782-4, 786
references.pmid: 11680708
references.citation: Luminescence. 2006 Jul-Aug;21(4):214-20
references.pmid: 16645959
references.citation: J Biolumin Chemilumin. 1991 Jul-Sep;6(3):189-92
references.pmid: 1746319
references.citation: Luminescence. 2001 Jul-Aug;16(4):295-8
references.pmid: 11512147
references.citation: Anal Biochem. 1989 May 15;179(1):206-8
references.pmid: 2757196
references.citation: Luminescence. 2013 Nov-Dec;28(6):915-21
references.pmid: 23225777
references.citation: Biotechniques. 1997 Apr;22(4):594-5
references.pmid: 9105599
references.citation: J Colloid Interface Sci. 2007 Nov 1;315(1):158-63
references.pmid: 17681516
references.citation: J Biolumin Chemilumin. 1990 Jan-Mar;5(1):53-6
references.pmid: 2156408
references.citation: Anal Bioanal Chem. 2012 Dec;404(10):3165-73
references.pmid: 23052873
references.citation: Luminescence. 2013 Sep-Oct;28(5):641-7
references.pmid: 23027659
references.citation: J Antimicrob Chemother. 1994 Sep;34(3):383-90
references.pmid: 7829412
references.citation: Appl Biochem Biotechnol. 2010 Feb;160(4):1065-73
references.pmid: 19347266
references.citation: Biosens Bioelectron. 2012 Feb 15;32(1):288-92
references.pmid: 22206786
references.citation: Photochem Photobiol. 2004 Mar;79(3):233-41
references.pmid: 15115295
references.citation: Talanta. 2012 Sep 15;99:643-8
references.pmid: 22967606
references.citation: Luminescence. 2004 Mar-Apr;19(2):94-115
references.pmid: 15098209
references.citation: Methods Mol Biol. 2013;927:363-76
references.pmid: 22992928
references.citation: Analyst. 2012 Oct 21;137(20):4802-8
references.pmid: 22910835
references.citation: J Chromatogr A. 2001 Jan 12;907(1-2):39-46
references.pmid: 11217046
references.citation: Anal Chim Acta. 2011 Feb 7;686(1-2):115-20
references.pmid: 21237316
references.citation: Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4
references.pmid: 388439
references.citation: Mech Ageing Dev. 1996 Mar 29;86(3):145-50
references.pmid: 8733109
references.citation: Anal Chem. 1995 Jun 15;67(12):499R-502R
references.pmid: 8686870
references.citation: Arerugi. 1995 Jul;44(7):661-9
references.pmid: 7575131
references.citation: Proc Natl Acad Sci U S A. 2004 Aug 17;101(33):12300-5
references.pmid: 15295099