title: [Preparation of monospecific antibodies against isoform 2 of translation elongation factor 1A (eEF1A2)].
abstract: Amino acid sequences of eukaryotic translation elongation factor isoform 1 (eEF1A1) and 2 (eEF1A2) were compared and two peptide fragments of eEF1A2 were chosen as linear antigenic determinants for generation of monospecific antipeptide antibodies. Selected peptides were synthesized, conjugated to bovine serum albumin (BSA) and used for mice immunizations. Antibodies, produced against the eEF1A2 fragment 330-343 conjugated to BSA, specifically recognized this isoform in the native and partially denatured states but did not interact with the eEF1A1 isoform. It was shown that these monospecific anti-eEF1A2 antibodies could be employed for eEF1A2 detection both by enzyme-linked immunosorbent assay and by immunoblotting.
authors.forename: E F
authors.initials: EF
authors.lastname: Kolesanova
authors.forename: T E
authors.initials: TE
authors.lastname: Farafonova
authors.forename: E Iu
authors.initials: EIu
authors.lastname: Aleshina
authors.forename: N V
authors.initials: NV
authors.lastname: Pyndyk
authors.forename: M V
authors.initials: MV
authors.lastname: Verem'eva
authors.forename: A V
authors.initials: AV
authors.lastname: Novosil'naia
authors.forename: M I
authors.initials: MI
authors.lastname: Kovalenko
authors.forename: V F
authors.initials: VF
authors.lastname: Shalak
authors.forename: B S
authors.initials: BS
authors.lastname: Negrutskiĭ
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D000918:Antibody Specificity; D015153:Blotting, Western / Q000379:methods*; D002417:Cattle; D006801:Humans; D007114:Immunization; D008099:Liver / Q000737:chemistry; D051379:Mice; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000737:chemistry; D020648:Peptide Elongation Factor 1 / Q000032:analysis* / Q000276:immunology; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry* / Q000276:immunology; D011817:Rabbits; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012710:Serum Albumin, Bovine / Q000737:chemistry
chemical_list: D000906:Antibodies; C094814:EEF1A1 protein, human; C503234:EEF1A2 protein, human; D020648:Peptide Elongation Factor 1; D010455:Peptides; D012710:Serum Albumin, Bovine