title: Next-generation sequencing and DNA replication in human cells: the future has arrived.
abstract: Accurate regulation of DNA replication ensures faithful transmission of eukaryotic genomes and maintenance of genomic stability and chromatin organization. However, by itself the replication process is a threat for both DNA and chromatin integrity. This becomes particularly relevant in cancer cells, where activated oncogenes induce replication-stress, including unscheduled initiation, fork stalling and collapse and, ultimately, genomic instability. Studies addressing the relationship between (epi)genome integrity and disease have been hampered by our poor knowledge of the mechanisms regulating where and when eukaryotic replication initiates. Recently developed genome-scale methods for the analysis of DNA replication in mammals will contribute to the identification of missing links between replication, chromatin regulation and genome stability in normal and cancer cells.
authors.affiliation: Department of Experimental Oncology, European Institute of Oncology, 20141 Milan, Italy.
authors.forename: Gaetano Ivan
authors.initials: GI
authors.lastname: Dellino
authors.forename: Pier Giuseppe
authors.initials: PG
authors.lastname: Pelicci
mesh_terms: D000818:Animals; D047369:Chromatin Immunoprecipitation; D002874:Chromosome Mapping; D004261:DNA Replication / Q000502:physiology*; D005786:Gene Expression Regulation; D023281:Genomics; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D009369:Neoplasms / Q000235:genetics / Q000378:metabolism; D051738:Origin Recognition Complex / Q000378:metabolism; D018741:Replication Origin; D014158:Transcription, Genetic; D015533:Transcriptional Activation
chemical_list: D051738:Origin Recognition Complex