title: Specific localization and measurement of hydrogen peroxide in Arabidopsis thaliana cell suspensions and protoplasts elicited by COS-OGA.
abstract: H2O2 acts as an important signaling molecule during plant/pathogen interactions but its study remains a challenge due to the current shortcomings in H2O2-responsive probes. In this work, ContPY1, a new molecular probe developed to specifically detect H2O2 was used to study the elicitation of Arabidopsis thaliana cells by a complex of chitosan oligomers (COS) and oligogalacturonides (OGA). The comparison of cell suspensions, protoplasts of cell suspensions and leaf protoplasts treated with different inhibitors gave indications on the potential sources of hydrogen peroxide in plant cells. The relative contribution of the cell wall, of membrane dehydrogenases and of peroxidases depended on cell type and treatment and proved to be variable. Our present protocol can be used to study hydrogen peroxide production in a large variety of plant species by simple protocol adaptation.
authors.affiliation: Département Valorisation des Productions Agricoles; Centre Wallon de Recherches Agronomiques; Gembloux, Belgium; Laboratoire de Chimie Organique et Médicinale; Université Catholique de Louvain; Louvain-la-Neuve, Belgium.
authors.forename: Quentin
authors.initials: Q
authors.lastname: Ledoux
authors.affiliation: Unité de Recherche en Biologie Cellulaire Végétale; Université de Namur; Namur, Belgium.
authors.forename: Pierre
authors.initials: P
authors.lastname: Van Cutsem
authors.affiliation: Laboratoire de Chimie Organique et Médicinale; Université Catholique de Louvain; Louvain-la-Neuve, Belgium.
authors.forename: Istvan E
authors.initials: IE
authors.lastname: Markό
authors.affiliation: Département Valorisation des Productions Agricoles; Centre Wallon de Recherches Agronomiques; Gembloux, Belgium.
authors.forename: Pascal
authors.initials: P
authors.lastname: Veys
mesh_terms: D017360:Arabidopsis / Q000737:chemistry* / Q000166:cytology; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000737:chemistry*; D015335:Molecular Probes / Q000737:chemistry; D019255:NADPH Oxidases / Q000378:metabolism; D010544:Peroxidases / Q000378:metabolism; D018515:Plant Leaves / Q000737:chemistry / Q000166:cytology; D011523:Protoplasts / Q000737:chemistry*
chemical_list: D015335:Molecular Probes; D048271:Chitosan; D006861:Hydrogen Peroxide; D010544:Peroxidases; D019255:NADPH Oxidases
keywords: Arabidopsis; Cell suspension; elicitor; hydrogen peroxide; microscopy; protoplast
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