title: Unfolding of in planta activity of anti-rep ribozyme in presence of a RNA silencing suppressor.
abstract: Antisense RNA ribozymes have intrinsic endonucleolytic activity to effect cleavage of the target RNA. However, this activity in vivo is often controlled by the dominance of antisense or other double-stranded RNA mechanism. In this work, we demonstrate the in planta activity of a hammerhead ribozyme designed to target rep-mRNA of a phytopathogen Mungbean Yellow Mosaic India virus (MYMIV) as an antiviral agent. We also found RNA-silencing is induced on introduction of catalytically active as well as inactive ribozymes. Using RNA-silencing suppressors (RSS), we demonstrate that the endonucleolytic activity of ribozymes is a true phenomenon, even while a mutated version may demonstrate a similar down-regulation of the target RNA. This helps to ease the confusion over the action mechanism of ribozymes in vivo.
authors.affiliation: Plant Molecular Biology Division, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi 110 067, India. Electronic address: sumonakarjee@gmail.com.
authors.forename: Sumona Karjee
authors.initials: SK
authors.lastname: Mishra
authors.affiliation: Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology, Delhi, Hauz Khas, New Delhi 110 016, India.
authors.forename: Ushasri
authors.initials: U
authors.lastname: Chilakamarthi
authors.affiliation: Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology, Delhi, Hauz Khas, New Delhi 110 016, India.
authors.forename: J K
authors.initials: JK
authors.lastname: Deb
authors.affiliation: Plant Molecular Biology Division, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi 110 067, India; Department of Genetics, University of Delhi, South Campus, Benito Juarez Marg, New Delhi 110021, India.
authors.forename: Sunil Kumar
authors.initials: SK
authors.lastname: Mukherjee
mesh_terms: D053839:Begomovirus / Q000235:genetics; D015152:Blotting, Northern; D004720:Endonucleases / Q000235:genetics / Q000378:metabolism; D007887:Fabaceae / Q000821:virology; D010935:Plant Diseases / Q000235:genetics / Q000821:virology; D018515:Plant Leaves / Q000235:genetics / Q000821:virology; D034622:RNA Interference; D016337:RNA, Catalytic / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014026:Nicotiana / Q000235:genetics / Q000821:virology; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D016337:RNA, Catalytic; D012333:RNA, Messenger; D018749:RNA, Plant; D012367:RNA, Viral; D014764:Viral Proteins; D004720:Endonucleases
keywords: Hammerhead ribozyme; In planta; Mungbean Yellow Mosaic India virus; RNA-silencing; RNA-silencing suppressor
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