title: Prenatal organophosphates exposure alternates the cleavage plane orientation of apical neural progenitor in developing neocortex.
abstract: Prenatal organophosphate exposure elicits long-term brain cytoarchitecture and cognitive function impairments, but the mechanism underlying the onset and development of neural progenitors remain largely unclear. Using precise positioned brain slices, we observed an alternated cleavage plane bias that emerged in the mitotic neural progenitors of embryonal neocortex with diazinion (DZN) and chlorpyrifos (CPF) pretreatment. In comparison with the control, DZN and CPF treatment induced decrease of vertical orientation, increase of oblique orientation, and increase of horizontal orientation. That is, the cleavage plane orientation bias had been rotated from vertical to horizontal after DZN and CPF treatment. Meanwhile, general morphology and mitotic index of the progenitors were unchanged. Acephate (ACP), another common organophosphate, had no significant effects on the cleavage plane orientation, cell morphology and mitotic index. These results represent direct evidence for the toxicity mechanism in onset multiplication of neural progenitors.
authors.affiliation: College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, China.
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Chen
authors.affiliation: College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, China.
authors.forename: Wei-Feng
authors.initials: WF
authors.lastname: Chen
authors.affiliation: College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, China.
authors.forename: Da-Wei
authors.initials: DW
authors.lastname: Wang
mesh_terms: D000818:Animals; D004390:Chlorpyrifos / Q000633:toxicity*; D003976:Diazinon / Q000633:toxicity*; D004622:Embryo, Mammalian; D005260:Female; D007279:Injections, Subcutaneous; D007306:Insecticides / Q000633:toxicity*; D008813:Mice, Inbred ICR; D008940:Mitotic Index; D019579:Neocortex / Q000187:drug effects* / Q000254:growth & development / Q000473:pathology; D058953:Neural Stem Cells / Q000187:drug effects / Q000473:pathology; D009474:Neurons / Q000187:drug effects / Q000473:pathology; D009946:Organothiophosphorus Compounds / Q000494:pharmacology; D063088:Phosphoramides / Q000494:pharmacology; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000139:chemically induced* / Q000473:pathology
chemical_list: D007306:Insecticides; D009946:Organothiophosphorus Compounds; D063088:Phosphoramides; C001969:acephate; D004390:Chlorpyrifos; D003976:Diazinon
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