title: Soluble HLA-I/peptide monomers mediate antigen-specific CD8 T cell activation through passive peptide exchange with cell-bound HLA-I molecules.
abstract: Accumulating evidence that serum levels of soluble class I HLA molecules (sHLA-I) can, under various pathological conditions, correlate with disease stage and/or patient survival, has stimulated interest in defining whether sHLA-I can exert immunological functions. However, despite a mounting number of publications suggesting the ability of sHLA-I to affect immune effectors in vitro, the precise underlying mechanism still remains controversial. In this article, we address potential functions of both classical and nonclassical sHLA-I, using soluble recombinant HLA-I/peptide monomers, and clearly demonstrate their ability to trigger Ag-specific activation of CD8 T cells in vitro. Furthermore, we provide strong evidence that this behavior results from the passive transfer of peptides from monomers to T cell-bound HLA-I molecules, allowing for fratricide representation and activation. Hence, we proposed a unifying model of T cell activation by HLA-I/peptide monomers, reappraising the potential involvement of sHLA-I molecules in the immune response.
authors.affiliation: INSERM, U892, Nantes F-44007, France; Centre National de la Recherche Scientifique, U6299, Nantes F-44007, France; Université de Nantes, Nantes F-44007 France; and.
authors.forename: Mathilde
authors.initials: M
authors.lastname: Allard
authors.affiliation: INSERM, U892, Nantes F-44007, France; Centre National de la Recherche Scientifique, U6299, Nantes F-44007, France; Université de Nantes, Nantes F-44007 France; and.
authors.forename: Romain
authors.initials: R
authors.lastname: Oger
authors.affiliation: INSERM, U892, Nantes F-44007, France; Centre National de la Recherche Scientifique, U6299, Nantes F-44007, France; Université de Nantes, Nantes F-44007 France; and.
authors.forename: Houssem
authors.initials: H
authors.lastname: Benlalam
authors.affiliation: INSERM, U892, Nantes F-44007, France; Centre National de la Recherche Scientifique, U6299, Nantes F-44007, France; Université de Nantes, Nantes F-44007 France; and.
authors.forename: Laetitia
authors.initials: L
authors.lastname: Florenceau
authors.affiliation: INSERM, U892, Nantes F-44007, France; Centre National de la Recherche Scientifique, U6299, Nantes F-44007, France; Université de Nantes, Nantes F-44007 France; and Recombinant Protein Production Facility, Federative Research Structure François Bonamy, Nantes, F-44007, France.
authors.forename: Klara
authors.initials: K
authors.lastname: Echasserieau
authors.affiliation: INSERM, U892, Nantes F-44007, France; Recombinant Protein Production Facility, Federative Research Structure François Bonamy, Nantes, F-44007, France.
authors.forename: Karine
authors.initials: K
authors.lastname: Bernardeau
authors.affiliation: INSERM, U892, Nantes F-44007, France; Centre National de la Recherche Scientifique, U6299, Nantes F-44007, France; Université de Nantes, Nantes F-44007 France; and.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Labarrière
authors.affiliation: INSERM, U892, Nantes F-44007, France; Centre National de la Recherche Scientifique, U6299, Nantes F-44007, France; Université de Nantes, Nantes F-44007 France; and Recombinant Protein Production Facility, Federative Research Structure François Bonamy, Nantes, F-44007, France.
authors.forename: François
authors.initials: F
authors.lastname: Lang
authors.affiliation: INSERM, U892, Nantes F-44007, France; Centre National de la Recherche Scientifique, U6299, Nantes F-44007, France; Université de Nantes, Nantes F-44007 France; and nadine.gervois@inserm.fr.
authors.forename: Nadine
authors.initials: N
authors.lastname: Gervois
mesh_terms: D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D005260:Female; D006367:HeLa Cells; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D006801:Humans; D008297:Male; D010455:Peptides / Q000276:immunology*; D021381:Protein Transport / Q000276:immunology; D012995:Solubility
chemical_list: D015395:Histocompatibility Antigens Class I; D010455:Peptides