title: Media optimization for elevated molecular weight and mass production of pigment-free pullulan.
abstract: In this study, an Aureobasidium pullulans SZU 1001 mutant, deficient in pigment production, was screened by complex UV and γ-ray mutagenesis. Medium composition optimization for increased pullulan molecular weight and production was conducted using this mutant. Six nutrients: yeast extract, (NH4)2SO4, K2HPO4, NaCl, MgSO4·7H2O and CaCl2 were detected within pullulan production in flasks. It is shown that NaCl and K2HPO4 have significant influences on molecular weight of pullulan, while yeast extract and (NH4)2SO4 significantly affect pullulan yield. To achieve a higher molecular weight and more efficient pullulan production, a response surface methodology approach was introduced to predict an optimal nutrient combination. A molecular weight of 5.74 × 10(6) and pullulan yield on glucose of 51.30% were obtained under batch pullulan fermentation with the optimized media, which increased molecular weight and pullulan production by 97.25% and 11.04%, respectively compared with the control media.
authors.affiliation: School of Biology and Basic Medical Sciences, Soochow University, Suzhou 215123, China.
authors.forename: Xiaoliu
authors.initials: X
authors.lastname: Yu
authors.forename: Yulei
authors.initials: Y
authors.lastname: Wang
authors.forename: Gongyuan
authors.initials: G
authors.lastname: Wei
authors.forename: Yingying
authors.initials: Y
authors.lastname: Dong
mesh_terms: D005936:Glucans / Q000737:chemistry*; D008970:Molecular Weight; D010860:Pigments, Biological / Q000737:chemistry*
chemical_list: D005936:Glucans; D010860:Pigments, Biological; C009109:pullulan