title: Human umbilical cord-derived mesenchymal stem cells inhibit proliferation but maintain survival of Jurkat leukemia cells in vitro by activating Notch signaling.
abstract: OBJECTIVE
  To investigate the effects of human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) on the proliferation and survival of Jurkat leukemia cells in vitro and explore the possible mechanism.
  
  
  METHODS
  Jurkat leukemia cells were co-cultured with hUC-MSCs isolated from human umbilical cord tissues by plastic adherence at a ratio of 10:1. The proliferation and survival of the co-cultured Jurkat cells, separated by immunomagnetic bead cell sorting on day 4, were evaluated by flow cytometry. Western blotting was performed to evaluate the activation of Notch signaling in the co-cultured Jurkat cells.
  
  
  RESULTS
  Jurkat leukemia cells co-cultured with hUC-MSCs for 4 days showed a lowered proliferation rate and cell cycle arrest at G0/G1 phase with a reduction in the cell apoptotic rate. Notch signaling pathway was activated in the co-cultured Jurkat cells as evidenced by an increased cellular expression of HES-1.
  
  
  CONCLUSIONS
  Co-culture with hUC-MSCs can inhibit the proliferation of Jurkat leukemia cells in vitro and protect the cells from apoptosis by activating Notch signaling, indicating a potential shielding effect of MSCs on leukemia cells.
authors.affiliation: School of Biosciences and Biopharmaceutics, Guangdong Province Key Laboratory for Biotechnology Drug Candidates, Guangdong Pharmaceutical University, Guangzhou 510006, China.E-mail: yinyinyuan@126.com.
authors.forename: Yin
authors.initials: Y
authors.lastname: Yuan
authors.forename: Danliang
authors.initials: D
authors.lastname: Chen
authors.forename: Xuan
authors.initials: X
authors.lastname: Chen
authors.forename: Hongwei
authors.initials: H
authors.lastname: Shao
authors.forename: Shulin
authors.initials: S
authors.lastname: Huang
mesh_terms: D017209:Apoptosis; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D018920:Coculture Techniques; D006801:Humans; D019169:Jurkat Cells; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051881:Receptor, Notch1 / Q000378:metabolism*; D015398:Signal Transduction; D014470:Umbilical Cord / Q000166:cytology*
chemical_list: C497761:NOTCH1 protein, human; D051881:Receptor, Notch1