title: Molecular identification of adulteration in mutton based on mitochondrial 16S rRNA gene.
abstract: The aim of this study is to set up a protocol for identification of the adulteration in mutton based on mitochondrial 16S rRNA gene. The multiplex polymerase chain reaction (multi-PCR) assay was carried out to trace the impure DNA in mutton. A universal primer pair yielded an approximate 610 bp fragment in mutton, pork, duck, chicken, horse and cat meats. The amplicons of multi-PCR assay represented the species-specific products, which could be discriminated by the size ranging from 106 bp to 532 bp. Subsequently, the authentication of each fragment was also confirmed by sequencing. Random analyses of adulterants with various meats yielded the identical results to their components, showing the suitability of the multi-PCR assay for tracing of adulterant meats with high-accuracy and precision. This assay was sensitive to detect the species-specific DNA in different proportional mixtures of mutton and duck/pork (9.1%-90.9%). In conclusion, this multi-PCR assay successfully discriminated the double-, triple-, quadruple-, and quintuple-mixtures containing variant counterparts. This method will be particularly useful in the detection of mutton adulteration in processed foods further.
authors.affiliation: a College of Animal Science and Technology, Sichuan Agricultural University , Ya'an , Sichuan , China .
authors.forename: Jia
authors.initials: J
authors.lastname: Xu
authors.affiliation: a College of Animal Science and Technology, Sichuan Agricultural University , Ya'an , Sichuan , China .
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
authors.affiliation: a College of Animal Science and Technology, Sichuan Agricultural University , Ya'an , Sichuan , China .
authors.forename: Mengru
authors.initials: M
authors.lastname: Zhu
authors.affiliation: a College of Animal Science and Technology, Sichuan Agricultural University , Ya'an , Sichuan , China .
authors.forename: Yuanju
authors.initials: Y
authors.lastname: Wen
authors.affiliation: a College of Animal Science and Technology, Sichuan Agricultural University , Ya'an , Sichuan , China .
authors.forename: Tao
authors.initials: T
authors.lastname: Xie
authors.affiliation: a College of Animal Science and Technology, Sichuan Agricultural University , Ya'an , Sichuan , China .
authors.forename: Xiaoqian
authors.initials: X
authors.lastname: He
authors.affiliation: a College of Animal Science and Technology, Sichuan Agricultural University , Ya'an , Sichuan , China .
authors.forename: Yongfeng
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: b College of Veterinary Medicine, Sichuan Agricultural University , Ya'an , Sichuan , China , and.
authors.forename: Suizhong
authors.initials: S
authors.lastname: Cao
authors.affiliation: a College of Animal Science and Technology, Sichuan Agricultural University , Ya'an , Sichuan , China .
authors.forename: Lili
authors.initials: L
authors.lastname: Niu
authors.affiliation: a College of Animal Science and Technology, Sichuan Agricultural University , Ya'an , Sichuan , China .
authors.forename: Hongping
authors.initials: H
authors.lastname: Zhang
authors.affiliation: c Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University , Chengdu , Sichuan , China.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhong
mesh_terms: D000818:Animals; D002415:Cats; D002645:Chickens / Q000235:genetics; D004372:Ducks / Q000235:genetics; D054629:Genome, Mitochondrial / Q000235:genetics*; D006736:Horses / Q000235:genetics; D008460:Meat; D008969:Molecular Sequence Data; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D013552:Swine / Q000235:genetics
chemical_list: D012336:RNA, Ribosomal, 16S
keywords: 16S rRNA; adulteration; mitochondria; multi-PCR; mutton