title: Dual channel sensitive detection of hsa-miR-21 based on rolling circle amplification and quantum dots tagging.
abstract: An isothermal, highly sensitive and specific assay for the detection of hsa-miR-21 with the integration of QDs tagging and rolling circle amplification was offered. In addition, a dual channel strategy for miRNA detection was proposed: anodic stripping voltammetry (ASV) and fluorescent method were both performed for the final Cd2+ signal readout. The designed strategy exhibited good specificity to hsa-miR-21 and presented comparable detection results by detection methods.
authors.forename: Dan-Chen
authors.initials: DC
authors.lastname: Wangt
authors.forename: Li-Hui
authors.initials: LH
authors.lastname: Hu
authors.forename: Yu-Hui
authors.initials: YH
authors.lastname: Zhou
authors.forename: Yu-Ting
authors.initials: YT
authors.lastname: Huang
authors.forename: Xinhua
authors.initials: X
authors.lastname: Li
authors.forename: Jun-Jie
authors.initials: JJ
authors.lastname: Zhu
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D003217:Conductometry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D035683:MicroRNAs / Q000032:analysis* / Q000235:genetics*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D045663:Quantum Dots; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000295:instrumentation*
chemical_list: C524940:MIRN21 microRNA, human; D035683:MicroRNAs