title: Neurotoxocarosis: marked preference of Toxocara canis for the cerebrum and T. cati for the cerebellum in the paratenic model host mouse.
abstract: BACKGROUND
  Infective larvae of the worldwide occurring zoonotic roundworm T. canis exhibit a marked affinity to the nervous tissues of paratenic hosts. In humans, most cases of neurotoxocarosis are considered to be caused by larvae of T. canis as T. cati larvae have rarely been found in the CNS in previous studies. However, direct comparison of studies is difficult as larval migration depends on a variety of factors including mouse strains and inoculation doses. Therefore, the present study aims to provide a direct comparison of both roundworm species in mice as a model for paratenic hosts with specific focus on the CNS during the acute and chronic phase of disease to provide a basis for further studies dealing with neurotoxocarosis.
  
  
  METHODS
  C57Bl/6J mice were infected with 2000 embryonated T. canis and T. cati eggs, respectively as well as Balb/c mice infected with T. cati eggs only. On 8 time points post infection, organs were removed and microscopically examined for respective larvae. Special focus was put on the CNS, including analysis of larval distribution in the cerebrum and cerebellum, right and left hemisphere as well as eyes and spinal cord. Additionally, brains of all infection groups as well as uninfected controls were examined histopathologically to characterize neurostructural damage.
  
  
  RESULTS
  Significant differences in larval distribution were observed between and within the infection groups during the course of infection. As expected, significantly higher recovery rates of T. canis than T. cati larvae were determined in the brain. Surprisingly, significantly more T. canis larvae could be found in cerebra of infected mice whereas T. cati larvae were mainly located in the cerebellum. Structural damage in brain tissue could be observed in all infection groups, being more severe in brains of T. canis infected mice.
  
  
  CONCLUSIONS
  The data obtained provides an extensive characterization of migrational routes of T. canis and T. cati in the paratenic host mouse in direct comparison. Even though to a lesser extent, structural damage in the brain was also caused by T. cati larvae and therefore, the potential as pathogenic agents should not be underestimated.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Janecek
authors.forename: Andreas
authors.initials: A
authors.lastname: Beineke
authors.forename: Thomas
authors.initials: T
authors.lastname: Schnieder
authors.affiliation: Institute for Parasitology, University of Veterinary Medicine Hannover, Buenteweg 17, 30559 Hannover, Germany. christina.strube@tiho-hannover.de.
authors.forename: Christina
authors.initials: C
authors.lastname: Strube
mesh_terms: D000818:Animals; D001927:Brain Diseases / Q000469:parasitology* / Q000473:pathology; D002531:Cerebellum / Q000469:parasitology*; D054022:Cerebrum / Q000469:parasitology*; D007814:Larva; D051379:Mice; D013045:Species Specificity; D014018:Tissue Distribution; D014119:Toxocara / Q000145:classification* / Q000502:physiology*; D014120:Toxocariasis / Q000469:parasitology* / Q000473:pathology
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