title: Development and validation of an analytical method for the separation and determination of major bioactive curcuminoids in Curcuma longa rhizomes and herbal products using non-aqueous capillary electrophoresis.
abstract: A simple, fast and efficient non-aqueous capillary electrophoresis method (NACE) was developed for the simultaneous determination of three major bioactive curcuminoids (CMNs) in Curcuma longa rhizomes and its herbal products. Good separation, resolution and reproducibility were achieved with the background electrolyte (BGE) consisting a mixture of 15.0 mM sodium tetraborate and 7.4 mM sodium hydroxide (NaOH) in 2:10:15 (v/v/v) of water, 1-propanol, and methanol. The influences of background electrolyte, sodium hydroxide, water, sodium dodecyl sulfate and hydroxylpropyl-β-cyclodextrin on separations were investigated. The separation was carried out in a fused-silica capillary tube with reverse polarity. Hydrodynamic injection of 25mbar for 12s was used for injecting samples and a voltage of 28 kV was applied for separation. The ultrasonication method was used for the extraction of CMNs from the turmeric herbal products and the extract was filtered and directly injected without any further treatments. The limits of detection and quantification were less than 5.0 and 14.6 µg/ml respectively for all CMNs. The percentage recoveries for CMNs were >97.2% (%RSD, <2.62). The results obtained by the method were compared with existing spectrophotometric and HPLC methods. The related compounds in the extract did not interfere in the determination of CMNs. The proposed NACE method is better than existing chromatographic and electrophoretic methods in terms of simple electrophoretic medium, fast analysis and good resolution.
authors.affiliation: Natural Product Chemistry Division, CSIR - Indian Institute of Chemical Technology, Hyderabad 500007, India.
authors.forename: S
authors.initials: S
authors.lastname: Anubala
authors.affiliation: Analytical Chemistry Division, CSIR - Indian Institute of Chemical Technology, Hyderabad 500007, India. Electronic address: seker@rediffmail.com.
authors.forename: R
authors.initials: R
authors.lastname: Sekar
authors.affiliation: Organic and Biomolecular Chemistry Division, CSIR - Indian Institute of Chemical Technology, Hyderabad 500007, India. Electronic address: nagaiah@iict.res.in.
authors.forename: K
authors.initials: K
authors.lastname: Nagaiah
mesh_terms: D000433:1-Propanol / Q000737:chemistry; D030024:Curcuma / Q000737:chemistry*; D003474:Curcumin / Q000031:analogs & derivatives / Q000032:analysis* / Q000302:isolation & purification; D003505:Cyclodextrins / Q000737:chemistry; D004573:Electrolytes; D019075:Electrophoresis, Capillary / Q000379:methods*; D000432:Methanol / Q000737:chemistry; D015394:Molecular Structure; D028321:Plant Preparations / Q000737:chemistry*; D015203:Reproducibility of Results; D027343:Rhizome / Q000737:chemistry*; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D012997:Solvents / Q000737:chemistry
chemical_list: D003505:Cyclodextrins; D004573:Electrolytes; D028321:Plant Preparations; D012997:Solvents; D012967:Sodium Dodecyl Sulfate; D000433:1-Propanol; D003474:Curcumin; D000432:Methanol
keywords: Curcuma longa rhizomes; Curcuminoids; Herbal products; Method development; NACE