title: Overexpression of SAMDC1 gene in Arabidopsis thaliana increases expression of defense-related genes as well as resistance to Pseudomonas syringae and Hyaloperonospora arabidopsidis.
abstract: It has been previously described that elevation of endogenous spermine levels in Arabidopsis could be achieved by transgenic overexpression of S-Adenosylmethionine decarboxylase (SAMDC) or Spermine synthase (SPMS). In both cases, spermine accumulation had an impact on the plant transcriptome, with up-regulation of a set of genes enriched in functional categories involved in defense-related processes against both biotic and abiotic stresses. In this work, the response of SAMDC1-overexpressing plants against bacterial and oomycete pathogens has been tested. The expression of several pathogen defense-related genes was induced in these plants as well as in wild type plants exposed to an exogenous supply of spermine. SAMDC1-overexpressing plants showed an increased tolerance to infection by Pseudomonas syringae and by Hyaloperonospora arabidopsidis. Both results add more evidence to the hypothesis that spermine plays a key role in plant resistance to biotic stress.
authors.affiliation: Departament de Biologia Vegetal, Facultat de Farmàcia, Universitat de València València, Spain.
authors.forename: Francisco
authors.initials: F
authors.lastname: Marco
authors.affiliation: Departament de Bioquímica i Biologia Molecular, Facultat de Ciències Biològiques, Universitat de València València, Spain.
authors.forename: Enrique
authors.initials: E
authors.lastname: Busó
authors.affiliation: Departament de Bioquímica i Biologia Molecular, Facultat de Ciències Biològiques, Universitat de València València, Spain.
authors.forename: Pedro
authors.initials: P
authors.lastname: Carrasco
keywords: biotic stress; jasmonate; polyamines; spermine; stress response and stress tolerance
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