title: Extracellular wire tetrode recording in brain of freely walking insects.
abstract: Increasing interest in the role of brain activity in insect motor control requires that we be able to monitor neural activity while insects perform natural behavior. We previously developed a technique for implanting tetrode wires into the central complex of cockroach brains that allowed us to record activity from multiple neurons simultaneously while a tethered cockroach turned or altered walking speed. While a major advance, tethered preparations provide access to limited behaviors and often lack feedback processes that occur in freely moving animals. We now present a modified version of that technique that allows us to record from the central complex of freely moving cockroaches as they walk in an arena and deal with barriers by turning, climbing or tunneling. Coupled with high speed video and cluster cutting, we can now relate brain activity to various parameters of the movement of freely behaving insects.
authors.affiliation: Department of Biology, Case Western Reserve University.
authors.forename: Peiyuan
authors.initials: P
authors.lastname: Guo
authors.affiliation: Department of Biology, Case Western Reserve University.
authors.forename: Alan J
authors.initials: AJ
authors.lastname: Pollack
authors.affiliation: Department of Biology, Case Western Reserve University.
authors.forename: Adrienn G
authors.initials: AG
authors.lastname: Varga
authors.affiliation: Department of Biology, Case Western Reserve University.
authors.forename: Joshua P
authors.initials: JP
authors.lastname: Martin
authors.affiliation: Department of Biology, Case Western Reserve University; roy.ritzmann@case.edu.
authors.forename: Roy E
authors.initials: RE
authors.lastname: Ritzmann
mesh_terms: D000818:Animals; D001921:Brain / Q000502:physiology*; D003058:Cockroaches / Q000502:physiology*; D004567:Electrodes, Implanted; D005260:Female; D008297:Male; D008839:Microelectrodes; D009474:Neurons / Q000502:physiology; D014741:Video Recording; D016138:Walking / Q000502:physiology*
references.citation: J Comp Physiol A Neuroethol Sens Neural Behav Physiol. 2008 Apr;194(4):341-60
references.pmid: 18180927
references.citation: Nat Neurosci. 2004 May;7(5):446-51
references.pmid: 15114356
references.citation: J Neurophysiol. 2004 Jul;92(1):236-54
references.pmid: 14985411
references.citation: J Neurosci. 2013 Feb 6;33(6):2443-56
references.pmid: 23392673
references.citation: Source Code Biol Med. 2009 Jul 22;4:5
references.pmid: 19624853
references.citation: J Neurosci. 2009 Apr 15;29(15):4911-21
references.pmid: 19369560
references.citation: J Exp Biol. 2013 Mar 15;216(Pt 6):992-1002
references.pmid: 23197098
references.citation: Curr Opin Neurobiol. 2011 Aug;21(4):527-34
references.pmid: 21705212
references.citation: J Comp Neurol. 2008 Dec 1;511(4):454-78
references.pmid: 18837039
references.citation: Science. 2007 Feb 16;315(5814):995-7
references.pmid: 17303756
references.citation: J Neurosci. 2009 Sep 23;29(38):11783-93
references.pmid: 19776265
references.citation: Curr Biol. 2010 Apr 13;20(7):663-8
references.pmid: 20346674
references.citation: Curr Opin Neurobiol. 2002 Dec;12(6):633-8
references.pmid: 12490252
references.citation: Curr Biol. 2010 May 25;20(10):921-6
references.pmid: 20451382
references.citation: Nat Methods. 2009 Jun;6(6):451-7
references.pmid: 19412169
references.citation: Neuron. 2011 Jan 27;69(2):345-58
references.pmid: 21262471
references.citation: IEEE Trans Biomed Circuits Syst. 2011 Apr;5(2):103-11
references.pmid: 23851198
references.citation: Curr Biol. 2005 Aug 23;15(16):1473-8
references.pmid: 16111941