title: Association of circulating endothelial microparticles with cardiometabolic risk factors in the Framingham Heart Study.
abstract: OBJECTIVE
  To examine the relation of endothelial microparticles (EMPs) with cardiometabolic risk in the community.
  
  
  BACKGROUND
  Circulating EMPs are small membrane vesicles released after endothelial cell injury. Endothelial microparticles are reportedly increased among individuals with a high burden of cardiovascular risk factors. However, prior investigations have been limited to small, highly selected samples.
  
  
  METHODS
  We studied 844 individuals without a history of cardiovascular disease in the Framingham Offspring cohort (mean age 66 ± 9 years, 57% women). We used standardized flow cytometry methods to identify and quantify circulating CD144+ and CD31+/CD41- EMPs. We then used multivariable regression analyses to investigate the relations of EMP phenotypes with cardiovascular and metabolic risk factors.
  
  
  RESULTS
  In multivariable analyses, the following cardiovascular risk factors were associated with one or more of the circulating EMP populations: hypertension (P = 0.025 for CD144+,), elevated triglycerides (P = 0.002 for CD144+, P < 0.0001 for CD31+/CD41-), and metabolic syndrome (P < 0.0001 for CD144+,). Overall, each tertile increase in the Framingham risk score corresponded to a 9% increase in log-CD31+/CD41- EMPs (P = 0.022). Furthermore, the presence of hypertriglyceridaemic waist status was associated with 38% higher levels of CD144+ EMPs (P < 0.0001) and 46% higher levels of CD31+/CD41- EMPs (P < 0.0001).
  
  
  CONCLUSIONS
  In a large community-based sample, circulating EMP levels were associated with the presence of cardiometabolic risk factors, particularly dyslipidaemia. These data underscore the potential influence of high-risk metabolic profiles on endothelial integrity.
authors.affiliation: INSERM, U970, Paris Cardiovascular Research Center - PARCC, 56 Rue Leblanc, 75737 Paris cedex 15, France Université Paris Descartes, Sorbonne Paris Cité, UMR-S970 Paris, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Amabile
authors.affiliation: Framingham Heart Study, Framingham, MA, USA Cardiovascular Division, Brigham and Women's Hospital, Boston, MA, USA.
authors.forename: Susan
authors.initials: S
authors.lastname: Cheng
authors.affiliation: INSERM, U970, Paris Cardiovascular Research Center - PARCC, 56 Rue Leblanc, 75737 Paris cedex 15, France Université Paris Descartes, Sorbonne Paris Cité, UMR-S970 Paris, France.
authors.forename: Jean Marie
authors.initials: JM
authors.lastname: Renard
authors.affiliation: Framingham Heart Study, Framingham, MA, USA Department of Mathematics and Statistics, Boston University, Boston, MA, USA.
authors.forename: Martin G
authors.initials: MG
authors.lastname: Larson
authors.affiliation: Cardiology Division, Massachusetts General Hospital, Boston, MA, USA.
authors.forename: Anahita
authors.initials: A
authors.lastname: Ghorbani
authors.affiliation: Department of Mathematics and Statistics, Boston University, Boston, MA, USA Cardiology Division, Massachusetts General Hospital, Boston, MA, USA.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: McCabe
authors.affiliation: Duke University School of Medicine, Durham, NC, USA.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Griffin
authors.affiliation: INSERM, U970, Paris Cardiovascular Research Center - PARCC, 56 Rue Leblanc, 75737 Paris cedex 15, France Université Paris Descartes, Sorbonne Paris Cité, UMR-S970 Paris, France.
authors.forename: Coralie
authors.initials: C
authors.lastname: Guerin
authors.affiliation: Framingham Heart Study, Framingham, MA, USA Cardiology Division, Massachusetts General Hospital, Boston, MA, USA.
authors.forename: Jennifer E
authors.initials: JE
authors.lastname: Ho
authors.affiliation: Cardiology Division, Massachusetts General Hospital, Boston, MA, USA Center for Systems Biology, Massachusetts General Hospital, Boston, MA, USA.
authors.forename: Stanley Y
authors.initials: SY
authors.lastname: Shaw
authors.affiliation: Section of Hematology/Oncology, University of Chicago Medical Center, Chicago, IL, USA.
authors.forename: Kenneth S
authors.initials: KS
authors.lastname: Cohen
authors.affiliation: Framingham Heart Study, Framingham, MA, USA Sections of Preventive Medicine and Cardiology, Boston University School of Medicine, Boston, MA, USA.
authors.forename: Ramachandran S
authors.initials: RS
authors.lastname: Vasan
authors.affiliation: INSERM, U970, Paris Cardiovascular Research Center - PARCC, 56 Rue Leblanc, 75737 Paris cedex 15, France Université Paris Descartes, Sorbonne Paris Cité, UMR-S970 Paris, France.
authors.forename: Alain
authors.initials: A
authors.lastname: Tedgui
authors.affiliation: INSERM, U970, Paris Cardiovascular Research Center - PARCC, 56 Rue Leblanc, 75737 Paris cedex 15, France Université Paris Descartes, Sorbonne Paris Cité, UMR-S970 Paris, France chantal.boulanger@inserm.fr thomas.j.wang@vanderbilt.edu.
authors.forename: Chantal M
authors.initials: CM
authors.lastname: Boulanger
authors.affiliation: Framingham Heart Study, Framingham, MA, USA Cardiology Division, Massachusetts General Hospital, Boston, MA, USA Division of Cardiovascular Medicine, Vanderbilt University Medical Center, 2220 Pierce Avenue, 383 Preston Research Building, Nashville, TN 37232, USA chantal.boulanger@inserm.fr thomas.j.wang@vanderbilt.edu.
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Wang
mesh_terms: D000368:Aged; D015703:Antigens, CD / Q000378:metabolism; D002318:Cardiovascular Diseases / Q000473:pathology*; D055252:Cell-Derived Microparticles / Q000473:pathology*; D042783:Endothelial Cells / Q000473:pathology; D004730:Endothelium, Vascular / Q000473:pathology*; D005260:Female; D006801:Humans; D008137:Longitudinal Studies; D008297:Male; D024821:Metabolic Syndrome / Q000473:pathology*; D012307:Risk Factors
chemical_list: D015703:Antigens, CD
keywords: Cardiovascular risk factors; Endothelium; Microparticles
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