title: Combined elevation of microRNA-196a and microRNA-196b in sera predicts unfavorable prognosis in patients with osteosarcomas.
abstract: OBJECTIVE
  To investigate whether the aberrant expression of microRNA (miR)-196a and miR-196b can be used as potential prognostic markers of human osteosarcoma.
  
  
  METHODS
  Quantitative real-time reverse transcriptase-polymerase chain reaction (qRT-PCR) analysis was performed to detect the expression levels of miR-196a and miR-196b in osteosarcoma tissues and patients' sera.
  
  
  RESULTS
  Expression levels of miR-196a and miR-196b in osteosarcoma tissues were both significantly higher than those in noncancerous bone tissues (both p<0.001), in line with which, the serum levels of the two miRNAs were also markedly upregulated in patients with osteosarcomas compared with healthy controls (both p<0.001). Then, the elevation of serum miR-196a and miR-196b levels both more frequently occurred in osteosarcoma patients with high tumor grade (p=0.008 and 0.01, respectively), positive metastasis (p=0.001 and 0.006, respectively) and recurrence (p=0.001 and 0.006, respectively). Moreover, high serum miR-196a, high serum miR-196b and conjoined expression of miR-196a/miR-196b were all independent prognostic factors for OS (overall survival) and DFS (disease-free survival) of osteosarcoma patients.
  
  
  CONCLUSIONS
  Our present data indicate the involvement of miR-196a and miR-196b upregulation in the pathogenesis of osteosarcoma. More importantly, the altered levels of circulating miR-196a and miR-196b might have great potential to serve as novel and non-invasive prognostic factors for this malignancy.
authors.affiliation: Department of Orthopedic Surgery, the Second Hospital Affiliated to Xi'an Jiao Tong University School of Medicine, Xi'an 710004, Shaanxi, China. zyt1117@126.com.
authors.forename: Chun
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Aesthetic Plastic Surgery, the Second Hospital Affiliated to Xi'an Jiao Tong University School of Medicine, Xi'an 710004, Shaanxi, China. 1113zyt@163.com.
authors.forename: Cong
authors.initials: C
authors.lastname: Yao
authors.affiliation: Department of Orthopedic Surgery, the Second Hospital Affiliated to Xi'an Jiao Tong University School of Medicine, Xi'an 710004, Shaanxi, China. lhp-3993@163.com.
authors.forename: Haopeng
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Orthopedic Surgery, the Second Hospital Affiliated to Xi'an Jiao Tong University School of Medicine, Xi'an 710004, Shaanxi, China. wgy1509@126.com.
authors.forename: Guoyu
authors.initials: G
authors.lastname: Wang
authors.affiliation: Department of Orthopedic Surgery, the Second Hospital Affiliated to Xi'an Jiao Tong University School of Medicine, Xi'an 710004, Shaanxi, China. xijingh@gmail.com.
authors.forename: Xijng
authors.initials: X
authors.lastname: He
mesh_terms: D000970:Antineoplastic Agents / Q000627:therapeutic use; D001859:Bone Neoplasms / Q000175:diagnosis / Q000188:drug therapy / Q000235:genetics; D018572:Disease-Free Survival; D005260:Female; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D016017:Odds Ratio; D012516:Osteosarcoma / Q000175:diagnosis / Q000188:drug therapy / Q000235:genetics; D011379:Prognosis; D012008:Recurrence; D015854:Up-Regulation
chemical_list: D000970:Antineoplastic Agents; C542543:MIRN196 microRNA, human; D035683:MicroRNAs
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