title: Are rice (Oryza sativa L.) phosphate transporters regulated similarly by phosphate and arsenate? A comprehensive study.
abstract: Rice is one of the most important staple foods worldwide, but it often contains inorganic arsenic, which is toxic and gives rise to severe health problems. Rice plants take up arsenate As(V) via the phosphate transport pathways, though it is not known how As(V), as compared to phosphate, modifies the expression of phosphate transporters (PTs). Therefore, the impact of As(V) or phosphate (Pi) on the gene expression of PTs and several Pi signaling regulators was investigated. Rice plants were grown on medium containing different As(V) or Pi concentrations. Growth was evaluated and the expression of tested genes was quantified at different time points, using quantitative RT-PCR (qPCR). The As and P content in plants was determined using inductively coupled plasma mass spectrometry (ICP-MS). As(V) elicited diverse and opposite responses of different PTs in roots and shoots, while Pi triggered a more shallow and uniform transcriptional response in several tested genes. Only a restricted set of genes, including PT2, PT3, PT5 and PT13 and two SPX-MFS family members, was particularly responsive to As(V). Despite some common reactions, the responses of the analyzed genes were predominantly ion-specific. The possible reasons and consequences are discussed.
authors.affiliation: Centre for Applied Geosciences, Geomicrobiology, University of Tuebingen, Sigwartstrasse 10, 72076, Tuebingen, Germany.
authors.forename: E Marie
authors.initials: EM
authors.lastname: Muehe
authors.forename: Jochen F
authors.initials: JF
authors.lastname: Eisele
authors.forename: Birgit
authors.initials: B
authors.lastname: Daus
authors.forename: Andreas
authors.initials: A
authors.lastname: Kappler
authors.forename: Klaus
authors.initials: K
authors.lastname: Harter
authors.forename: Christina
authors.initials: C
authors.lastname: Chaban
mesh_terms: D001149:Arsenates / Q000494:pharmacology*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D012275:Oryza / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D028061:Phosphate Transport Proteins / Q000235:genetics / Q000378:metabolism*; D010710:Phosphates / Q000494:pharmacology*; D018520:Plant Shoots / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D036226:Seedlings / Q000254:growth & development; D012639:Seeds; D015398:Signal Transduction
chemical_list: D001149:Arsenates; D028061:Phosphate Transport Proteins; D010710:Phosphates; D018749:RNA, Plant
references.citation: Plant Physiol. 2011 Sep;157(1):269-78
references.pmid: 21753117
references.citation: Sci Rep. 2013;3:2195
references.pmid: 23873074
references.citation: Sci Total Environ. 2007 Jul 1;379(2-3):180-9
references.pmid: 17067657
references.citation: Plant J. 2009 Mar;57(5):798-809
references.pmid: 18980647
references.citation: J Exp Bot. 2008;59(8):2267-76
references.pmid: 18453530
references.citation: Front Physiol. 2012 Apr 02;3:55
references.pmid: 22536187
references.citation: Environ Sci Pollut Res Int. 2013 Feb;20(2):884-96
references.pmid: 23054772
references.citation: New Phytol. 2012 Oct;196(1):139-148
references.pmid: 22803610
references.citation: Mol Plant. 2011 Nov;4(6):1105-22
references.pmid: 21832284
references.citation: Front Physiol. 2012 Jun 06;3:182
references.pmid: 22685440
references.citation: J Integr Plant Biol. 2008 Jul;50(7):835-48
references.pmid: 18713394
references.citation: Biochemistry. 2011 Apr 5;50(13):2701-9
references.pmid: 21348499
references.citation: Environ Sci Technol. 2005 Aug 1;39(15):5531-40
references.pmid: 16124284
references.citation: Plant Physiol. 1998 Feb 1;116(2):447-53
references.pmid: 9490752
references.citation: Plant Mol Biol. 2010 Mar;72(4-5):423-32
references.pmid: 19936943
references.citation: Environ Sci Technol. 2012 Aug 7;46(15):8333-40
references.pmid: 22765219
references.citation: Plant J. 1997 Jan;11(1):83-92
references.pmid: 9025304
references.citation: J Biochem Mol Toxicol. 2006;20(1):48-56
references.pmid: 16498640
references.citation: Proc Natl Acad Sci U S A. 1996 Sep 17;93(19):10519-23
references.pmid: 8927627
references.citation: Science. 2003 May 9;300(5621):939-44
references.pmid: 12738852
references.citation: Plant Mol Biol. 2012 Dec;80(6):587-608
references.pmid: 22987115
references.citation: Ann Bot. 2006 Sep;98(3):631-6
references.pmid: 16803848
references.citation: Plant Physiol. 2000 Mar;122(3):793-801
references.pmid: 10712543
references.citation: Annu Rev Plant Biol. 2011;62:185-206
references.pmid: 21370979
references.citation: Environ Sci Technol. 2009 Mar 1;43(5):1612-7
references.pmid: 19350943
references.citation: J Environ Biol. 2005 Jul;26(3):551-5
references.pmid: 16334296
references.citation: Chemosphere. 2007 Aug;68(10):1906-12
references.pmid: 17416405
references.citation: Toxicol Sci. 2010 Oct;117(2):270-81
references.pmid: 20457661
references.citation: Chemosphere. 2009 Feb;74(5):688-702
references.pmid: 18996570
references.citation: Plant Physiol. 2010 Mar;152(3):1693-704
references.pmid: 20081045
references.citation: Plant J. 2010 May;62(3):508-17
references.pmid: 20149131
references.citation: Proc Natl Acad Sci U S A. 2002 Oct 1;99(20):13324-9
references.pmid: 12271140
references.citation: Plant Physiol. 2011 Jul;156(3):1101-15
references.pmid: 21317339
references.citation: BMC Biol. 2008 Jun 10;6:26
references.pmid: 18544156
references.citation: New Phytol. 2012 Jul;195(1):97-112
references.pmid: 22537016
references.citation: Plant Cell. 2005 Dec;17(12):3500-12
references.pmid: 16284308
references.citation: Phytochemistry. 2005 Jul;66(13):1519-28
references.pmid: 15964037
references.citation: Environ Sci Technol. 2011 Oct 1;45(19):8328-35
references.pmid: 21870848
references.citation: Plant Cell. 1997 Mar;9(3):381-92
references.pmid: 9090882
references.citation: Plant Physiol. 2001 May;126(1):299-306
references.pmid: 11351093
references.citation: Plant Cell Physiol. 2010 Dec;51(12):2119-31
references.pmid: 21062869
references.citation: J Integr Plant Biol. 2008 Jul;50(7):849-59
references.pmid: 18713395
references.citation: Curr Opin Plant Biol. 2000 Jun;3(3):182-7
references.pmid: 10837272
references.citation: Plant Physiol. 2011 Jun;156(2):913-24
references.pmid: 21490163
references.citation: Proc Natl Acad Sci U S A. 1999 May 11;96(10):5868-72
references.pmid: 10318976
references.citation: Plant Physiol. 2011 Sep;157(1):498-508
references.pmid: 21715673
references.citation: Annu Rev Plant Physiol Plant Mol Biol. 1999 Jun;50:665-693
references.pmid: 15012223
references.citation: Inorg Chem. 1999 Feb 22;38(4):818-820
references.pmid: 11670848
references.citation: Trends Plant Sci. 2004 Sep;9(9):415-7
references.pmid: 15337490
references.citation: Proteomics. 2008 Sep;8(17):3561-76
references.pmid: 18752204
references.citation: Environ Sci Technol. 2003 Sep 15;37(18):4182-9
references.pmid: 14524451
references.citation: Plant Cell. 2007 Mar;19(3):1123-33
references.pmid: 17400898
references.citation: Plant Physiol. 2012 Aug;159(4):1571-81
references.pmid: 22649273
references.citation: Plant Physiol. 2011 Jul;156(3):1164-75
references.pmid: 21502185
references.citation: Talanta. 2002 Aug 16;58(1):57-65
references.pmid: 18968734
references.citation: J Environ Sci (China). 2009;21(3):346-51
references.pmid: 19634447
references.citation: New Phytol. 2009 Mar;181(4):777-794
references.pmid: 19207683
references.citation: FEMS Microbiol Rev. 2000 Dec;24(5):625-45
references.pmid: 11077155
references.citation: J Biol Chem. 1983 May 25;258(10):6266-71
references.pmid: 6853484
references.citation: Plant J. 2009 Mar;57(5):895-904
references.pmid: 19000161
references.citation: Environ Sci Technol. 2008 Aug 1;42(15):5574-9
references.pmid: 18754478
references.citation: Plant Physiol. 2008 Apr;146(4):1673-86
references.pmid: 18263782
references.citation: Biochemistry. 2011 Feb 22;50(7):1128-34
references.pmid: 21214261
references.citation: Proc Natl Acad Sci U S A. 2008 Jul 22;105(29):9931-5
references.pmid: 18626020
references.citation: Biochemistry. 1993 Dec 7;32(48):13212-9
references.pmid: 8241176
references.citation: Plant Physiol. 1998 Jan;116(1):91-9
references.pmid: 9449838
references.citation: Environ Toxicol. 2004 Feb;19(1):26-34
references.pmid: 14758591
references.citation: New Phytol. 2012 Mar;193(4):842-51
references.pmid: 22403821
references.citation: Talanta. 2008 Apr 15;75(2):376-9
references.pmid: 18371894
references.citation: Plant J. 2003 Sep;35(5):637-46
references.pmid: 12940956