title: [Microcalorimetry -- a new method for bacterial characterisation].
abstract: The microcalorimetry is a method used for recording of the heat produced by a thermodinamic system in a scale of micronanojouls. One of the domains in which this method is used is the one called bacterial microcalorimetry, which studies the heat generated by the bacterial populations. The process of bacterial growth can be monitored in real time by the recording a graph of the generated power over time. The modern isothermal microcalorimeters allow the detection of a signal variation of only one microwatt. The estimated generated power of a bacteria is approximately 1-4pW thus only a small number of bacteria is necessary for the experiments. Recent studies in the field of bacterial microcalorimetry have demonstrated that, in standard conditions, this method can be reproductible and can be used to detect and characterize bacterial growth (through the study of the microcalorimetric growth curve particular to a bacterial species which is called a microcalorimetric fingerprint) and offers the new information in regards to bacterial metabolism. Also, microcalorimetry can offer information about bacterial interaction with different factors in the medium (for example, antibioticsubstances, in which case an antibiogram is obtained in 4-5 hours). In conclusion, we can say that microcalorimetry is a reproducible method, which offers an interesting perspective on bacterial characterization, with great scientific potential, and there are sufficient arguments to continue studies in this field.
authors.forename: Dragoş C
authors.initials: DC
authors.lastname: Zaharia
authors.forename: Mihnea G
authors.initials: MG
authors.lastname: Popa
authors.forename: Alexandru T
authors.initials: AT
authors.lastname: Steriade
authors.forename: Alexandru A
authors.initials: AA
authors.lastname: Muntean
authors.forename: Octavian
authors.initials: O
authors.lastname: Balint
authors.forename: Roxana
authors.initials: R
authors.lastname: Micuţ
authors.forename: Vlad T
authors.initials: VT
authors.lastname: Popa
authors.forename: Mircea I
authors.initials: MI
authors.lastname: Popa
authors.forename: Miron A
authors.initials: MA
authors.lastname: Bogdan
mesh_terms: D001419:Bacteria / Q000378:metabolism*; D018407:Bacterial Physiological Phenomena; D001431:Bacteriological Techniques; D002151:Calorimetry / Q000379:methods*; D006358:Hot Temperature; D015203:Reproducibility of Results; D013997:Time Factors