title: Expression pattern of the human ABC transporters in pluripotent embryonic stem cells and in their derivatives.
abstract: BACKGROUND
  ATP-binding cassette (ABC) transporters have key roles in various physiological functions as well as providing chemical defense and stress tolerance in human tissues. In this study, we have examined the expression pattern of all ABC proteins in pluripotent human embryonic stem cells (hESCs) and in their differentiated progenies. We paid special attention to the cellular expression and localization of multidrug transporter ABC proteins.
  
  
  METHODS
  Stem cell differentiation was carried out without chemical induction or cell sorting, and specialized cell types were separated mechanically. Cellular features regarding pluripotency and tissue identity, as well as ABC transporter expression were studied by flow cytomtery, immuno-microscopy, and qPCR-based low-density arrays.
  
  
  RESULTS
  Pluripotent hESCs and differentiated cell types (cardiomyocytes, neuronal cells, and mesenchymal stem cells) were distinguished by morphology, immunostaining markers, and selected mRNA expression patterns. We found that the mRNA expression levels of the 48 human ABC proteins also clearly distinguished the pluripotent and the respective differentiated cell types. When multidrug and lipid transporter ABC protein expression was examined by using well characterized specific antibodies by flow cytometry and confocal microscopy, the protein expression data corresponded well to the mRNA expression results. Moreover, the cellular localization of these important human ABC transporter proteins could be established in the pluripotent and differentiated hESC derived samples.
  
  
  CONCLUSIONS
  These studies provide valuable information regarding ABC protein expression in human stem cells and their differentiated offspring. The results may also help to obtain further information concerning the specialized cellular functions of selected ABC transporters.
authors.affiliation: Institute of Molecular Pharmacology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Budapest, Hungary.
authors.forename: Zsuzsa
authors.initials: Z
authors.lastname: Erdei
authors.forename: Réka
authors.initials: R
authors.lastname: Lőrincz
authors.forename: Kornélia
authors.initials: K
authors.lastname: Szebényi
authors.forename: Adrienn
authors.initials: A
authors.lastname: Péntek
authors.forename: Nóra
authors.initials: N
authors.lastname: Varga
authors.forename: István
authors.initials: I
authors.lastname: Likó
authors.forename: György
authors.initials: G
authors.lastname: Várady
authors.forename: Gergely
authors.initials: G
authors.lastname: Szakács
authors.forename: Tamás I
authors.initials: TI
authors.lastname: Orbán
authors.forename: Balázs
authors.initials: B
authors.lastname: Sarkadi
authors.forename: Agota
authors.initials: A
authors.lastname: Apáti
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism*; D005434:Flow Cytometry; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology; D018613:Microscopy, Confocal; D032383:Myocytes, Cardiac / Q000166:cytology; D009474:Neurons / Q000166:cytology; D039904:Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis
chemical_list: D018528:ATP-Binding Cassette Transporters; D012333:RNA, Messenger
keywords: ABC transporters; cardiomyocyte; flow cytometry; human pluripotent stem cell; mesenchymal cell; microscopy; neural cells; pluripotency; stem cells