title: "Click" immobilization of a VEGF-mimetic peptide on decellularized endothelial extracellular matrix to enhance angiogenesis.
abstract: We show that coating of decellularized extracellular matrix (DC-ECM) on substrate surfaces is an efficient way to generate a platform mimicking the native ECM environment. Moreover, the DC-ECM can be modified with a peptide (QK) mimicking vascular endothelial growth factor without apparently compromising its integrity. The modification was achieved through metabolic incorporation of a "clickable" handle to DC-ECM followed by rapid attachment of the QK peptide with an azido tag using copper-catalyzed click reaction. The attachment of the QK peptide on to DC-ECM in this way further enhanced the angiogenic responses (formation of branched tubular networks) of endothelial cells.
authors.affiliation: Department of Chemistry, University of Houston , Houston, Texas 77204, United States.
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.forename: Meirong
authors.initials: M
authors.lastname: Zhao
authors.forename: Siheng
authors.initials: S
authors.lastname: Li
authors.forename: Uriel J
authors.initials: UJ
authors.lastname: Erasquin
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Ren
authors.forename: Changyi
authors.initials: C
authors.lastname: Chen
authors.forename: Yingjun
authors.initials: Y
authors.lastname: Wang
authors.forename: Chengzhi
authors.initials: C
authors.lastname: Cai
mesh_terms: D057930:Click Chemistry; D004730:Endothelium, Vascular / Q000166:cytology*; D005109:Extracellular Matrix / Q000737:chemistry*; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D018716:Molecular Mimicry; D018919:Neovascularization, Physiologic; D010455:Peptides / Q000737:chemistry*; D042461:Vascular Endothelial Growth Factor A / Q000737:chemistry*
chemical_list: D010455:Peptides; D042461:Vascular Endothelial Growth Factor A
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