title: Correlation between Expression of Glucose Transporters in Granulosa Cells and Oocyte Quality in Women with Polycystic Ovary Syndrome.
abstract: BACKGROUND
  The glucose transporters (GLUTs) exhibit different tissue-specific expression. This study aimed to investigate the types of GLUTs expressed in human granulosa cells (GCs) obtained from women with polycystic ovary syndrome (PCOS) and their relationship with insulin resistance (IR) and the outcomes of in vitro maturation (IVM) of immature oocytes.
  
  
  METHODS
  Expression of GLUTs was evaluated in GCs from women with PCOS with or without IR. Thirty-six women with PCOS undergoing an IVM program were included. Differential gene expression between the insulin sensitive (IS) and IR group was measured by reverse transcription polymerase chain reaction.
  
  
  RESULTS
  Expression of GLUTs 1, 3, 5, 8, and 13 was constitutive, whereas expression of GLUTs 2 and 7 was not observed in human GCs. The remaining GLUTs, 4, 6, 9, 10, 11, and 12, were differentially expressed among patients according to metabolic status, such as insulin sensitivity. A higher number of GCs from patients with IR (92%) expressed GLUT6 than GCs from IS PCOS patients (46.3%). Logistic regression showed that expression of GLUTs 9, 11, and 12 correlates with rates of IVM at 48 hours, fertilization, and implantation, respectively.
  
  
  CONCLUSIONS
  This is the first report describing the expression pattern of all 13 members of the GLUT family in human GCs. Results of the present study suggest that patients' insulin sensitivity regulates GLUT expression in GCs in PCOS patients, and this may control oocyte quality for IVM and subsequent processes such as fertilization and implantation in patients taking part in an in vitro fertilization program.
authors.affiliation: Department of Biomedical Science, CHA University, Seoul, Korea.
authors.forename: Eunju
authors.initials: E
authors.lastname: Kim
authors.affiliation: Fertility Center, CHA Gangnam Medical Center, CHA University, Seoul, Korea.
authors.forename: Hyun Ha
authors.initials: HH
authors.lastname: Seok
authors.affiliation: Department of Biomedical Science, CHA University, Seoul, Korea.
authors.forename: Su-Yeon
authors.initials: SY
authors.lastname: Lee
authors.affiliation: Department of Biomedical Science, CHA University, Seoul, Korea. ; Fertility Center, CHA Gangnam Medical Center, CHA University, Seoul, Korea.
authors.forename: Dong Ryul
authors.initials: DR
authors.lastname: Lee
authors.affiliation: Department of Applied Bioscience, CHA University, Seoul, Korea.
authors.forename: Jisook
authors.initials: J
authors.lastname: Moon
authors.affiliation: Fertility Center, CHA Gangnam Medical Center, CHA University, Seoul, Korea.
authors.forename: Tae Ki
authors.initials: TK
authors.lastname: Yoon
authors.affiliation: Fertility Center, CHA Gangnam Medical Center, CHA University, Seoul, Korea.
authors.forename: Woo Sik
authors.initials: WS
authors.lastname: Lee
authors.affiliation: Department of Biomedical Science, CHA University, Seoul, Korea. ; Fertility Center, CHA Gangnam Medical Center, CHA University, Seoul, Korea.
authors.forename: Kyung-Ah
authors.initials: KA
authors.lastname: Lee
keywords: Glucose transport proteins, facilitative; Granulosa cells; In vitro maturation; Polycystic ovary syndrome
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