title: Five withanolides from the leaves of Datura metel L. and their inhibitory effects on nitric oxide production.
abstract: Four new withanolides named dmetelins A-D (compounds 1-4), along with the known compound 7α,27-dihydroxy-1-oxo-witha-2,5,24-trienolide (5) were isolated from the leaves of Datura metel L. (Solanaceae). Their structures were elucidated on the basis of detailed analysis of 1D and 2D NMR and mass spectrometry data. All the compounds were evaluated for their inhibitory effects on lipopolysaccharide (LPS)-induced nitric oxide (NO) production in RAW264.7 cells. Compounds 1, 4 and 5 showed significant inhibitory activities, and compounds 2 and 3 showed moderate inhibitory activities with IC50 values of 17.8, 11.6, 14.9, 33.3 and 28.6 μM, respectively.
authors.affiliation: Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Ministry of Education, Harbin 150040, China.
authors.forename: Bing-You
authors.initials: BY
authors.lastname: Yang
authors.affiliation: Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Ministry of Education, Harbin 150040, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Guo
authors.affiliation: Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Ministry of Education, Harbin 150040, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Li
authors.affiliation: Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Ministry of Education, Harbin 150040, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Ministry of Education, Harbin 150040, China.
authors.forename: Chang-Fu
authors.initials: CF
authors.lastname: Wang
authors.affiliation: Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Ministry of Education, Harbin 150040, China.
authors.forename: Zun-Peng
authors.initials: ZP
authors.lastname: Shu
authors.affiliation: Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Ministry of Education, Harbin 150040, China.
authors.forename: Zhi-Bin
authors.initials: ZB
authors.lastname: Wang
authors.affiliation: Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Ministry of Education, Harbin 150040, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Ministry of Education, Harbin 150040, China.
authors.forename: Yong-Gang
authors.initials: YG
authors.lastname: Xia
authors.affiliation: Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Ministry of Education, Harbin 150040, China.
authors.forename: Hai
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Ministry of Education, Harbin 150040, China. qhwang668@sina.com.
authors.forename: Qiu-Hong
authors.initials: QH
authors.lastname: Wang
authors.affiliation: Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Ministry of Education, Harbin 150040, China. hxkuang@hotmail.com.
authors.forename: Hai-Xue
authors.initials: HX
authors.lastname: Kuang
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D002460:Cell Line; D058530:Datura metel / Q000737:chemistry*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D015394:Molecular Structure; D009569:Nitric Oxide / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry*; D054358:Withanolides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D000893:Anti-Inflammatory Agents; D008070:Lipopolysaccharides; D010936:Plant Extracts; D054358:Withanolides; D009569:Nitric Oxide
references.citation: Life Sci. 2003 Nov 21;74(1):125-32
references.pmid: 14575818
references.citation: Evid Based Complement Alternat Med. 2013;2013:352019
references.pmid: 23606878
references.citation: Phytochemistry. 2003 Jun;63(4):387-90
references.pmid: 12770585
references.citation: Anal Biochem. 1982 Oct;126(1):131-8
references.pmid: 7181105
references.citation: Planta Med. 1984 Apr;50(2):134-6
references.pmid: 6548033
references.citation: Planta Med. 1997 Feb;63(1):15-7
references.pmid: 9063090
references.citation: Phytochemistry. 2002 Mar;59(6):635-41
references.pmid: 11867095
references.citation: J Org Chem. 2003 Mar 21;68(6):2350-61
references.pmid: 12636402
references.citation: J Nat Prod. 2011 Dec 27;74(12):2532-44
references.pmid: 22098611
references.citation: Chem Biodivers. 2006 Feb;3(2):180-6
references.pmid: 17193256
references.citation: Molecules. 2011 Jul 11;16(7):5833-9
references.pmid: 21747337
references.citation: J Nat Prod. 2013 Jan 25;76(1):22-8
references.pmid: 23316950
references.citation: J Nat Prod. 2011 May 27;74(5):1132-41
references.pmid: 21425785
references.citation: J Nat Prod. 2010 Nov 29;73(11):1898-906
references.pmid: 20961091
references.citation: Arch Pharm Res. 2010 Aug;33(8):1143-8
references.pmid: 20803115
references.citation: Planta Med. 2010 Jan;76(1):82-5
references.pmid: 19653148
references.citation: Phytother Res. 2014 Mar;28(3):395-404
references.pmid: 23640962
references.citation: Steroids. 2010 Apr;75(4-5):346-9
references.pmid: 20116389