title: Assessment of microRNAs in patients with unstable angina pectoris.
abstract: OBJECTIVE
  While cardiac troponin measurements have significantly improved the early diagnosis of myocardial infarction, the timely biomarker-based diagnosis of unstable angina pectoris (UAP) remains a major unmet clinical challenge. The aim of this study was to assess levels of circulating microRNAs (miRNAs) as possible novel biomarkers in patients with UAP.
  
  
  RESULTS
  A three-phase approach was conducted, comprising (i) profiling of miRNAs in patients with UAP and controls groups; (ii) replication of significant miRNAs in an independent patient cohort, (iii) validation of a multi-miRNAs panel in a third cohort. Out of 25 miRNAs selected for replication, 8 miRNAs remained significantly associated with UAP. In a validation phase, a miRNA panel including miR-132, miR-150, and miR-186 showed the highest discriminatory power [area under the receiver-operating-characteristic curve (AUC): 0.91; CI: 0.84-0.98].
  
  
  CONCLUSIONS
  Using a profiling-replication-validation model, we identified eight miRNAs, which may facilitate the diagnosis of UAP.
authors.affiliation: Department of General and Interventional Cardiology, University Heart Center Hamburg, Martinistr. 52, 20246 Hamburg, Germany German Center for Cardiovascular Research (DZHK), Partner Site Hamburg, Lübeck, Kiel, Hamburg, Germany.
authors.forename: Tanja
authors.initials: T
authors.lastname: Zeller
authors.affiliation: Department of General and Interventional Cardiology, University Heart Center Hamburg, Martinistr. 52, 20246 Hamburg, Germany German Center for Cardiovascular Research (DZHK), Partner Site Rhein-Main, Mainz, Germany.
authors.forename: Till
authors.initials: T
authors.lastname: Keller
authors.affiliation: Department of General and Interventional Cardiology, University Heart Center Hamburg, Martinistr. 52, 20246 Hamburg, Germany.
authors.forename: Francisco
authors.initials: F
authors.lastname: Ojeda
authors.affiliation: Department of Internal Medicine, University Hospital, Basel, Switzerland Department of Cardiology, University Hospital, Basel, Switzerland.
authors.forename: Tobias
authors.initials: T
authors.lastname: Reichlin
authors.affiliation: Department of Internal Medicine, University Hospital, Basel, Switzerland Department of Cardiology, University Hospital, Basel, Switzerland.
authors.forename: Raphael
authors.initials: R
authors.lastname: Twerenbold
authors.affiliation: Department of Medicine II, University Medical Center, Johannes Gutenberg University Mainz, Mainz, Germany.
authors.forename: Stergios
authors.initials: S
authors.lastname: Tzikas
authors.affiliation: German Center for Cardiovascular Research (DZHK), Partner Site Rhein-Main, Mainz, Germany Department of Medicine II, University Medical Center, Johannes Gutenberg University Mainz, Mainz, Germany Clinical Epidemiology, Center for Thrombosis and Haemostasis, University Medical Center Mainz, Langenbeckstraße 1, 55131 Mainz, Germany.
authors.forename: Philipp S
authors.initials: PS
authors.lastname: Wild
authors.affiliation: Department of Internal Medicine, University Hospital, Basel, Switzerland Department of Cardiology, University Hospital, Basel, Switzerland.
authors.forename: Miriam
authors.initials: M
authors.lastname: Reiter
authors.affiliation: Department of Medicine II, University Medical Center, Johannes Gutenberg University Mainz, Mainz, Germany.
authors.forename: Ewa
authors.initials: E
authors.lastname: Czyz
authors.affiliation: German Center for Cardiovascular Research (DZHK), Partner Site Rhein-Main, Mainz, Germany Department of Laboratory Medicine, University Medical Center, Johannes Gutenberg University Mainz, Germany.
authors.forename: Karl J
authors.initials: KJ
authors.lastname: Lackner
authors.affiliation: German Center for Cardiovascular Research (DZHK), Partner Site Rhein-Main, Mainz, Germany Department of Medicine II, University Medical Center, Johannes Gutenberg University Mainz, Mainz, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Munzel
authors.affiliation: Department of Internal Medicine, University Hospital, Basel, Switzerland Department of Cardiology, University Hospital, Basel, Switzerland.
authors.forename: Christian
authors.initials: C
authors.lastname: Mueller
authors.affiliation: Department of General and Interventional Cardiology, University Heart Center Hamburg, Martinistr. 52, 20246 Hamburg, Germany German Center for Cardiovascular Research (DZHK), Partner Site Hamburg, Lübeck, Kiel, Hamburg, Germany s.blankenberg@uke.de.
authors.forename: Stefan
authors.initials: S
authors.lastname: Blankenberg
mesh_terms: D000328:Adult; D000368:Aged; D000789:Angina, Unstable / Q000175:diagnosis*; D016022:Case-Control Studies; D042241:Early Diagnosis; D005260:Female; D005819:Genetic Markers; D005821:Genetic Techniques; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D008875:Middle Aged; D009203:Myocardial Infarction / Q000175:diagnosis; D012372:ROC Curve; D015203:Reproducibility of Results
chemical_list: D005819:Genetic Markers; D035683:MicroRNAs
keywords: Acute myocardial infarction; Circulating microRNA; Diagnosis; Myocardial infarction; Unstable angina pectoris