title: Dynamics of filopodium-like protrusion and endothelial cellular motility on one-dimensional extracellular matrix fibrils.
abstract: Endothelial filopodia play key roles in guiding the tubular sprouting during angiogenesis. However, their dynamic morphological characteristics, with the associated implications in cell motility, have been subjected to limited investigations. In this work, the interaction between endothelial cells and extracellular matrix fibrils was recapitulated in vitro, where a specific focus was paid to derive the key morphological parameters to define the dynamics of filopodium-like protrusion during cell motility. Based on one-dimensional gelatin fibrils patterned by near-field electrospinning (NFES), we study the response of endothelial cells (EA.hy926) under normal culture or ROCK inhibition. It is shown that the behaviour of temporal protrusion length versus cell motility can be divided into distinct modes. Persistent migration was found to be one of the modes which permitted cell displacement for over 300 µm at a speed of approximately 1 µm min(-1). ROCK inhibition resulted in abnormally long protrusions and diminished the persistent migration, but dramatically increased the speeds of protrusion extension and retraction. Finally, we also report the breakage of protrusion during cell motility, and examine its phenotypic behaviours.
authors.affiliation: Cavendish Laboratory , University of Cambridge , Cambridge CB3 0HE , UK.
authors.forename: Niannan
authors.initials: N
authors.lastname: Xue
authors.affiliation: Cavendish Laboratory , University of Cambridge , Cambridge CB3 0HE , UK.
authors.forename: Cristina
authors.initials: C
authors.lastname: Bertulli
authors.affiliation: The Institute of Cancer Research , 237 Fulham Road, London SW3 6JB , UK.
authors.forename: Amine
authors.initials: A
authors.lastname: Sadok
authors.affiliation: Department of Engineering , University of Cambridge , Cambridge CB2 1PZ , UK.
authors.forename: Yan Yan Shery
authors.initials: YY
authors.lastname: Huang
keywords: ROCK; cell motility; cellular protrusion; extracellular matrix; micropatterning; near-field electrospinning
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