title: [Microwave radiation induces injury to GC-2spd cells].
abstract: OBJECTIVE
  To explore the impact of microwave radiation on GC-2spd cells.
  
  
  METHODS
  We exposed cultured GC-2spd cells to microwave radiation at the average power densities of 0, 10 and 30 mW/cm2 for 15 minutes and, from I to 24 hours after the exposure, we observed the changes in cell proliferation, histology and ultrastructure, cell apoptosis, and cAMP content by MTIT, light microscopy, electron microscopy, flow cytometry and ELISA.
  
  
  RESULTS
  Compared with the control group, the GC-2spd cells showed a significant decrease in proliferation ability at 1 -24 hours after 10 and 30 mW/cm2 microwave radiation, except at 12 hours after 30 mW/cm2 radiation (P <0.05 or P <0.01), with reduced length and number of cell enation and increased intra cytoplasm vacuoles. The rate of cell apoptosis (%) was significantly increased in the 10 and 30 mW/cm2 groups at 6 hours (4.56 +/- 2.09 vs 14.59 +/- 1.09 and 8.48 +/- 1.73, P <0.05 or P <0.01) , with agglutination and margin translocation of chromatins and obvious dilation of endo cytoplasmic reticula. The cAMP content (nmol/g) in the GC-2spd cells was remarkably reduced in the 10 and 30 mW/cm2 groups at 6 and 24 hours (2.77 +/-0.24 vs 1.65+/- 0. 17 and 1.96+/-0.10, 3.02 +/-0.47 vs 2.13 +/-0.33 and 1.69 +/-0.27, P <0.05 or P <0.01).
  
  
  CONCLUSIONS
  Microwave radiation at 10 and 30 mW/cm2 may cause injury to GC-2spd cells, which is manifested by decreased content of intracellular cAMP, reduced activity of cell proliferation, and increased rate of cell apoptosis.
authors.forename: Lei
authors.initials: L
authors.lastname: Xue
authors.forename: Shui-Ming
authors.initials: SM
authors.lastname: Wang
authors.forename: Hao-Yu
authors.initials: HY
authors.lastname: Chen
authors.forename: Rui-Yun
authors.initials: RY
authors.lastname: Peng
authors.forename: Hong-Yan
authors.initials: HY
authors.lastname: Zuo
authors.forename: Li-Feng
authors.initials: LF
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Zhao
authors.forename: Shao-Xia
authors.initials: SX
authors.lastname: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000528:radiation effects; D002460:Cell Line / Q000528:radiation effects; D049109:Cell Proliferation / Q000528:radiation effects; D008297:Male; D051379:Mice; D008872:Microwaves / Q000009:adverse effects*; D013090:Spermatocytes / Q000528:radiation effects*