title: [Irreversible chemical AFM-fishing for the detection of low-copied proteins].
abstract: The atomic-force microscopy-based method of irreversible chemical AFM-fishing (AFM-IF(Ch)) has been developed for the detection of proteins at ultra-low concentrations in solution. Using this method, a very low concentration of horseradish peroxidase (HRP) protein (10(-17) M) was detected in solution. A theoretical model that allows the description of obtained experimental data, is proposed. This model takes into consideration both the transport of the protein from the bulk solution onto the AFM-chip surface and its irreversible binding to the activated area.
authors.forename: Iu D
authors.initials: IuD
authors.lastname: Ivanov
authors.forename: V V
authors.initials: VV
authors.lastname: Danichev
authors.forename: T O
authors.initials: TO
authors.lastname: Pleshakova
authors.forename: I D
authors.initials: ID
authors.lastname: Shumov
authors.forename: V S
authors.initials: VS
authors.lastname: Ziborov
authors.forename: N V
authors.initials: NV
authors.lastname: Krokhin
authors.forename: M N
authors.initials: MN
authors.lastname: Zagumennyĭ
authors.forename: V S
authors.initials: VS
authors.lastname: Ustinov
authors.forename: L P
authors.initials: LP
authors.lastname: Smirnov
authors.forename: A V
authors.initials: AV
authors.lastname: Shironin
authors.forename: A I
authors.initials: AI
authors.lastname: Archakov
mesh_terms: D001360:Avidin / Q000302:isolation & purification*; D003198:Computer Simulation; D004527:Egg Proteins / Q000302:isolation & purification*; D006735:Horseradish Peroxidase / Q000302:isolation & purification*; D007700:Kinetics; D056656:Lab-On-A-Chip Devices; D018625:Microscopy, Atomic Force / Q000295:instrumentation / Q000379:methods*; D008956:Models, Chemical; D011485:Protein Binding; D012996:Solutions
chemical_list: D004527:Egg Proteins; D012996:Solutions; D001360:Avidin; D006735:Horseradish Peroxidase