title: Three-dimensional protein networks assembled by two-photon activation.
abstract: Spatial and temporal control over chemical and biological processes plays a key role in life and material sciences. Here we synthesized a two-photon-activatable glutathione (GSH) to trigger the interaction with glutathione S-transferase (GST) by light at superior spatiotemporal resolution. The compound shows fast and well-confined photoconversion into the bioactive GSH, which is free to interact with GST-tagged proteins. The GSH/GST interaction can be phototriggered, changing its affinity over several orders of magnitude into the nanomolar range. Multiplexed three-dimensional (3D) protein networks are simultaneously generated in situ through two-photon fs-pulsed laser-scanning excitation. The two-photon activation facilitates the three-dimensional assembly of protein structures in real time at hitherto unseen resolution in time and space, thus opening up new applications far beyond the presented examples.
authors.affiliation: Institute of Biochemistry, Biocenter, Goethe-University Frankfurt, Max-von-Laue-Strasse 9, 60438 Frankfurt am Main (Germany) http://www.biochem.uni-frankfurt.de.
authors.forename: Volker
authors.initials: V
authors.lastname: Gatterdam
authors.forename: Radhan
authors.initials: R
authors.lastname: Ramadass
authors.forename: Tatjana
authors.initials: T
authors.lastname: Stoess
authors.forename: Manuela A H
authors.initials: MA
authors.lastname: Fichte
authors.forename: Josef
authors.initials: J
authors.lastname: Wachtveitl
authors.forename: Alexander
authors.initials: A
authors.lastname: Heckel
authors.forename: Robert
authors.initials: R
authors.lastname: Tampé
mesh_terms: D004579:Electron Transport; D031541:Fluorescence Resonance Energy Transfer; D005978:Glutathione / Q000737:chemistry / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism*; D008027:Light; D017785:Photons; D054730:Protein Interaction Domains and Motifs
chemical_list: D005982:Glutathione Transferase; D005978:Glutathione
keywords: immobilization; photochemistry; protein-protein interactions; surface chemistry; two-photon activation