title: Mandibular reconstruction using a calcium phosphate/polyethylene glycol hydrogel carrier with BMP-2.
abstract: OBJECTIVE
  To test the hypothesis that a synthetic hydroxyapatite/β-tricalcium phosphate (HA/TCP) construct combined with polyethylene glycol (PEG) hydrogel including recombinant human bone morphogenetic proteins-2 (rhBMP-2) enhances new bone formation compared with bone morphogenetic proteins-2 (BMP-2) delivered using the HA/TCP construct alone.
  
  
  METHODS
  Bilateral mandibular partial thickness 20 × 8 × 8 mm (L × W × H) alveolar defects were surgically created in the edentulated posterior mandible in 18 female minipigs. Randomized into two groups of nine animals each, the alveolar defects either received HA/TCP or HA/TCP/PEG with or without BMP-2 (105 μg/defect) in contra-lateral sites using a split-mouth design. Primary outcome, bone density (%) within four regions of interest, was evaluated following a 4-week healing interval when the animals were killed for histometric analysis.
  
  
  RESULTS
  Bone morphogenetic proteins-2 loaded onto HA/TCP constructs significantly enhanced new bone formation compared with HA/TCP controls. Adding PEG apparently obstructed BMP-2 induced bone formation.
  
  
  CONCLUSIONS
  Polyethylene glycol compromises the osteogenic effect of BMP-2.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Georgia Augusta University, Goettingen, Germany.
authors.forename: Rudolf M
authors.initials: RM
authors.lastname: Gruber
authors.forename: Sebastian
authors.initials: S
authors.lastname: Krohn
authors.forename: Corinna
authors.initials: C
authors.lastname: Mauth
authors.forename: Michel
authors.initials: M
authors.lastname: Dard
authors.forename: Aart
authors.initials: A
authors.lastname: Molenberg
authors.forename: Katharina
authors.initials: K
authors.lastname: Lange
authors.forename: Christina
authors.initials: C
authors.lastname: Perske
authors.forename: Henning
authors.initials: H
authors.lastname: Schliephake
mesh_terms: D016301:Alveolar Bone Loss / Q000601:surgery; D000539:Alveolar Process / Q000187:drug effects / Q000473:pathology; D000818:Animals; D001672:Biocompatible Materials / Q000627:therapeutic use; D015519:Bone Density / Q000187:drug effects; D055396:Bone Morphogenetic Protein 2 / Q000627:therapeutic use*; D018786:Bone Substitutes / Q000627:therapeutic use*; D002130:Calcium Phosphates / Q000627:therapeutic use*; D004337:Drug Carriers; D005260:Female; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000627:therapeutic use*; D007576:Jaw, Edentulous, Partially / Q000601:surgery; D008334:Mandible / Q000187:drug effects / Q000473:pathology; D008336:Mandibular Diseases / Q000601:surgery*; D063175:Mandibular Reconstruction / Q000379:methods*; D010012:Osteogenesis / Q000187:drug effects; D011897:Random Allocation; D011994:Recombinant Proteins / Q000627:therapeutic use; D013552:Swine; D013556:Swine, Miniature; D016212:Transforming Growth Factor beta / Q000627:therapeutic use*
chemical_list: D001672:Biocompatible Materials; D055396:Bone Morphogenetic Protein 2; D018786:Bone Substitutes; D002130:Calcium Phosphates; D004337:Drug Carriers; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; C485817:beta-tricalcium phosphate; C499469:recombinant human bone morphogenetic protein-2; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate
keywords: bone morphogenetic protein 2; jawbone defects; maxillomandibular reconstruction; polyethylene glycol hydrogel