title: Photocrosslinking, micropatterning and cell adhesion studies of sodium hyaluronate with a trisdiazonium salt.
abstract: Chemically unmodified sodium hyaluronate has been crosslinked by photoinduced decomposition of a trifunctional diazonium salt to generate new biomaterials. In addition, the photocrosslinking process does not require a photoinitiator. Thin films of formulations of sodium hyaluronate and the photocrosslinker at different percentages have been processed. Cytotoxicity has been explored and toxicity was not observed with the selected cell lines. 2D patterns of controlled geometry have been generated by direct laser writing to perform cell adhesion studies. Different adhesion behavior of the cell lines, as assessed by vinculin immunostaining and scanning electron microscopy, has been observed in the polymeric films depending on the degree of photocrosslinking.
authors.affiliation: Instituto de Ciencia de Materiales de Aragón (ICMA), CSIC-Universidad de Zaragoza, Departamento de Química Orgánica, Facultad de Ciencias, C./Pedro Cerbuna 12, 50009 Zaragoza, Spain.
authors.forename: Miguel
authors.initials: M
authors.lastname: Lomba
authors.affiliation: Instituto de Ciencia de Materiales de Aragón (ICMA), CSIC-Universidad de Zaragoza, Departamento de Química Orgánica, Facultad de Ciencias, C./Pedro Cerbuna 12, 50009 Zaragoza, Spain.
authors.forename: Luis
authors.initials: L
authors.lastname: Oriol
authors.affiliation: Instituto de Ciencia de Materiales de Aragón (ICMA), CSIC-Universidad de Zaragoza, Departamento de Física de la Materia Condensada, Facultad de Ciencias, C./Pedro Cerbuna 12, 50009 Zaragoza, Spain.
authors.forename: Carlos
authors.initials: C
authors.lastname: Sánchez
authors.affiliation: Instituto Universitario de Nanociencia de Aragón, Campus Río Ebro (Edificio I+D), 50018 Zaragoza, Spain.
authors.forename: Valeria
authors.initials: V
authors.lastname: Grazú
authors.affiliation: Instituto Universitario de Nanociencia de Aragón, Campus Río Ebro (Edificio I+D), 50018 Zaragoza, Spain; Instituto Aragonés de Ciencias de la Salud, Avenida Gómez Laguna, 50009 Zaragoza, Spain.
authors.forename: Berta Sáez
authors.initials: BS
authors.lastname: Gutiérrez
authors.affiliation: Instituto Universitario de Nanociencia de Aragón, Campus Río Ebro (Edificio I+D), 50018 Zaragoza, Spain.
authors.forename: José Luis
authors.initials: JL
authors.lastname: Serrano
authors.affiliation: Instituto Universitario de Nanociencia de Aragón, Campus Río Ebro (Edificio I+D), 50018 Zaragoza, Spain.
authors.forename: Jesús Martínez
authors.initials: JM
authors.lastname: De la Fuente
mesh_terms: D000818:Animals; D019556:COS Cells; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002522:Chlorocebus aethiops; D003432:Cross-Linking Reagents / Q000737:chemistry* / Q000494:pharmacology; D003979:Diazonium Compounds / Q000737:chemistry* / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D003432:Cross-Linking Reagents; D003979:Diazonium Compounds; D006820:Hyaluronic Acid