title: A GFP promoter fusion library for the study of Salmonella biofilm formation and the mode of action of biofilm inhibitors.
abstract: Salmonella, an important foodborne pathogen, forms biofilms in many different environments. The composition of these biofilms differs depending on the growth conditions, and their development is highly coordinated in time. To develop efficient treatments, it is therefore essential that biofilm formation and its inhibition be understood in different environments and in a time-dependent manner. Many currently used techniques, such as transcriptomics or proteomics, are still expensive and thus limited in their application. Therefore, a GFP-promoter fusion library with 79 important Salmonella biofilm genes was developed (covering among other things matrix production, fimbriae and flagella synthesis, and c-di-GMP regulation). This library is a fast, inexpensive, and easy-to-use tool, and can therefore be conducted in different experimental setups in a time-dependent manner. In this paper, four possible applications are highlighted to illustrate and validate the use of this reporter fusion library.
authors.affiliation: a Department of Microbial and Molecular Systems, Centre of Microbial and Plant Genetics , KU Leuven , Leuven , Belgium.
authors.forename: S C A
authors.initials: SC
authors.lastname: Robijns
authors.forename: S
authors.initials: S
authors.lastname: Roberfroid
authors.forename: S
authors.initials: S
authors.lastname: Van Puyvelde
authors.forename: B
authors.initials: B
authors.lastname: De Pauw
authors.forename: E
authors.initials: E
authors.lastname: Uceda Santamaría
authors.forename: A
authors.initials: A
authors.lastname: De Weerdt
authors.forename: D
authors.initials: D
authors.lastname: De Coster
authors.forename: K
authors.initials: K
authors.lastname: Hermans
authors.forename: S C J
authors.initials: SC
authors.lastname: De Keersmaecker
authors.forename: J
authors.initials: J
authors.lastname: Vanderleyden
authors.forename: H P L
authors.initials: HP
authors.lastname: Steenackers
mesh_terms: D018441:Biofilms / Q000187:drug effects / Q000254:growth & development*; D057219:Biofouling / Q000517:prevention & control; D015723:Gene Library; D005798:Genes, Bacterial; D049452:Green Fluorescent Proteins / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012475:Salmonella / Q000502:physiology*
chemical_list: D049452:Green Fluorescent Proteins
keywords: FACS; GFP; Salmonella; biofilm; mode of action; reporter fusions