title: Strain development and optimized fermentation conditions for blood meal using Aspergillus niger and Aspergillus oryzae.
abstract: To hydrolyze blood meal (BM) effectively, two mutants were generated using ultra-violet mutagenesis. Single-factor experiments, the Plackett-Burman technique and response surface methodology were used to optimize the fermentation conditions. This study successfully generated a mutant and innovatively provided important parameters for utilizing BM by fermentation, which could be of industrial value.
authors.affiliation: Laboratory of Biotechnology, Institute of Animal Nutrition, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Yanbin
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Laboratory of Biotechnology, Institute of Animal Nutrition, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Laboratory of Biotechnology, Institute of Animal Nutrition, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Deben
authors.initials: D
authors.lastname: Wang
authors.affiliation: Laboratory of Biotechnology, Institute of Animal Nutrition, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Gao
authors.affiliation: Laboratory of Biotechnology, Institute of Animal Nutrition, Northeast Agricultural University, Harbin 150030, China. Electronic address: asshan@neau.edu.cn.
authors.forename: Anshan
authors.initials: A
authors.lastname: Shan
mesh_terms: D000821:Animal Feed; D001234:Aspergillus niger / Q000235:genetics / Q000378:metabolism*; D001236:Aspergillus oryzae / Q000235:genetics / Q000378:metabolism*; D002244:Carbon; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D016296:Mutagenesis; D009584:Nitrogen; D013696:Temperature
chemical_list: D002244:Carbon; D009584:Nitrogen
keywords: Aspergillus niger; Aspergillus oryzae; Blood meal; Fermentation optimization