title: RGD-conjugated UV-crosslinked chitosan scaffolds inoculated with mesenchymal stem cells for bone tissue engineering.
abstract: Biomimetic chitosan scaffolds were prepared using two types of chitosan derivatives, one containing photoreactive azides for UV-crosslinking and the other tethered with RGD peptides. Mesenchymal stem cells (MSCs) isolated from rat bone marrow were cultured in the RGD-conjugated, UV-crosslinked chitosan scaffolds for bone tissue engineering. RGD-incorporation to the chitosan-based scaffolds increased the cell contents from 2.4×10(4) to 3.8×10(4) cells/scaffold and 3.4×10(4) to 5.1×10(4) cells/scaffold after 1 and 10 days of culture, respectively. Furthermore, osteogenic differentiation of MSCs, indicated by ALP activity and expression of Runx2 and osteocalcin genes, was enhanced on the RGD-conjugated surface compared with the unmodified surfaces. After 14 days of osteogenic culture, calcium deposition in the RGD-conjugated scaffolds (711 nmol Ca/scaffold) was significantly higher than the control (390 nmol Ca/scaffold). The results demonstrate a potential application of RGD-immobilized, crosslinked chitosan scaffolds for bone tissue engineering applications.
authors.affiliation: Department of Chemical Engineering, National Taiwan University, No. 1, Roosevelt Rd., Sec. 4, Taipei 106, Taiwan.
authors.forename: Wei-Bor
authors.initials: WB
authors.lastname: Tsai
authors.affiliation: Department of Chemical Engineering, National Taiwan University, No. 1, Roosevelt Rd., Sec. 4, Taipei 106, Taiwan; Graduate Institute of Biotechnology and Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, No. 1, Sec. 3, ZhongXiao E. Rd., Taipei 106, Taiwan.
authors.forename: Yi-Ru
authors.initials: YR
authors.lastname: Chen
authors.affiliation: Department of Biomedical Engineering, Chung Yuan Christian University, Chungli, Taoyuan, Taiwan.
authors.forename: Wen-Tyng
authors.initials: WT
authors.lastname: Li
authors.affiliation: R&D Center for Membrane Technology and Department of Chemical Engineering, Chung Yuan Christian University, Chungli, Taoyuan, Taiwan.
authors.forename: Juin-Yih
authors.initials: JY
authors.lastname: Lai
authors.affiliation: Graduate Institute of Biotechnology and Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, No. 1, Sec. 3, ZhongXiao E. Rd., Taipei 106, Taiwan.
authors.forename: Hsuan-Liang
authors.initials: HL
authors.lastname: Liu
mesh_terms: D000818:Animals; D001386:Azides / Q000737:chemistry; D001842:Bone and Bones; D002454:Cell Differentiation; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry*; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics; D005260:Female; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D009842:Oligopeptides / Q000737:chemistry*; D015675:Osteocalcin / Q000235:genetics; D017208:Rats, Wistar; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds; D014466:Ultraviolet Rays
chemical_list: D001386:Azides; D050659:Core Binding Factor Alpha 1 Subunit; D009842:Oligopeptides; C493717:Runx2 protein, rat; D015675:Osteocalcin; C047981:arginyl-glycyl-aspartic acid; D048271:Chitosan