title: Simultaneous saccharification and fermentation and a consolidated bioprocessing for Hinoki cypress and Eucalyptus after fibrillation by steam and subsequent wet-disk milling.
abstract: An advanced pretreatment method that combines steam treatment (ST) with wet disk milling (WDM) was evaluated using two different species of woods, viz., Hinoki cypress (softwood) and Eucalyptus (hardwood). Bioconversion of the pretreated products was performed using enzymatic saccharification via a commercial cellulase mixture and two types of fermentation processing, i.e., yeast-based simultaneous saccharification and fermentation (SSF) and Clostridium thermocellum-based consolidated bioprocessing (CBP). A higher yield of glucose was obtained in the enzymatic saccharification and fermentation products from SSF and CBP with pretreatment consisting of WDM after ST, as compared to either ST or WDM alone. Maximum ethanol production via SSF and CBP were 359.3 and 79.4 mg/g-cellulose from Hinoki cypress, and 299.5 and 73.1 mg/g-cellulose from Eucalyptus, respectively. While the main fermentation product generated in CBP was acetate, the total products yield was 319.9 and 262.0 mg/g-cellulose from Hinoki cypress and Eucalyptus, respectively.
authors.affiliation: Biomass Refinery Research Center, National Institute of Advanced Industrial Science and Technology (AIST), 3-11-32 Kagamiyama, Higashi-Hiroshima, Hiroshima 737-0046, Japan.
authors.forename: Akio
authors.initials: A
authors.lastname: Kumagai
authors.affiliation: Biomass Refinery Research Center, National Institute of Advanced Industrial Science and Technology (AIST), 3-11-32 Kagamiyama, Higashi-Hiroshima, Hiroshima 737-0046, Japan.
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Kawamura
authors.affiliation: Biomass Refinery Research Center, National Institute of Advanced Industrial Science and Technology (AIST), 3-11-32 Kagamiyama, Higashi-Hiroshima, Hiroshima 737-0046, Japan; Department of Forest Biomaterials Engineering, College of Forest and Environmental Sciences, Kangwon National University, 192-1 Hyoja, Chuncheon 200-701, South Korea.
authors.forename: Seung-Hwan
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Biomass Refinery Research Center, National Institute of Advanced Industrial Science and Technology (AIST), 3-11-32 Kagamiyama, Higashi-Hiroshima, Hiroshima 737-0046, Japan. Electronic address: t-endo@aist.go.jp.
authors.forename: Takashi
authors.initials: T
authors.lastname: Endo
authors.affiliation: Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831-6226, USA.
authors.forename: Miguel
authors.initials: M
authors.lastname: Rodriguez
authors.affiliation: BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN 37831-6226, USA. Electronic address: biofuels4me@gmail.com.
authors.forename: Jonathan R
authors.initials: JR
authors.lastname: Mielenz
mesh_terms: D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D050260:Carbohydrate Metabolism; D002480:Cellulase / Q000378:metabolism; D029780:Chamaecyparis / Q000737:chemistry*; D005052:Eucalyptus / Q000737:chemistry*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D013227:Steam; D013997:Time Factors; D014867:Water / Q000737:chemistry*
chemical_list: D013227:Steam; D014867:Water; D002480:Cellulase; D005947:Glucose
keywords: Consolidated bioprocessing; Enzymatic saccharification; Simultaneous saccharification and fermentation; Steam treatment; Wet disk milling