title: The structure of ferricytochrome c552 from the psychrophilic marine bacterium Colwellia psychrerythraea 34H.
abstract: Approximately 40% of all proteins are metalloproteins, and approximately 80% of Earth's ecosystems are at temperatures ≤5 °C, including 90% of the global ocean. Thus, an essential aspect of marine metallobiochemistry is an understanding of the structure, dynamics, and mechanisms of cold adaptation of metalloproteins from marine microorganisms. Here, the molecular structure of the electron-transfer protein cytochrome c552 from the psychrophilic marine bacterium Colwellia psychrerythraea 34H has been determined by X-ray crystallography (PDB: ). The structure is highly superimposable with that of the homologous cytochrome from the mesophile Marinobacter hydrocarbonoclasticus. Based on structural analysis and comparison of psychrophilic, psychrotolerant, and mesophilic sequences, a methionine-based ligand-substitution mechanism for psychrophilic protein stabilization is proposed.
authors.affiliation: Department of Chemistry, Barnard College, Columbia University, 3009 Broadway, New York NY 10027, USA. jmagyar@barnard.edu.
authors.forename: Paul B
authors.initials: PB
authors.lastname: Harvilla
authors.forename: Holly N
authors.initials: HN
authors.lastname: Wolcott
authors.forename: John S
authors.initials: JS
authors.lastname: Magyar
mesh_terms: D034241:Alteromonadaceae / Q000737:chemistry*; D001426:Bacterial Proteins / Q000737:chemistry*; D018360:Crystallography, X-Ray; D003574:Cytochrome c Group / Q000737:chemistry*; D008958:Models, Molecular; D011487:Protein Conformation
chemical_list: D001426:Bacterial Proteins; D003574:Cytochrome c Group; C022302:cytochrome C-552
references.citation: J Inorg Biochem. 2014 Feb;131:76-8
references.pmid: 24275750
references.citation: Int J Mol Sci. 2012;13(9):11643-65
references.pmid: 23109875
references.citation: Biochemistry. 1998 Apr 14;37(15):5118-28
references.pmid: 9548742
references.citation: J Comput Chem. 2004 Oct;25(13):1605-12
references.pmid: 15264254
references.citation: Science. 2010 Oct 8;330(6001):204-8
references.pmid: 20736401
references.citation: ISME J. 2013 Nov;7(11):2091-104
references.pmid: 23788333
references.citation: Chembiochem. 2013 Sep 23;14(14):1828-38
references.pmid: 23908017
references.citation: Proc Natl Acad Sci U S A. 2005 Dec 20;102(51):18397-402
references.pmid: 16344477
references.citation: Inorg Chem. 2011 Dec 5;50(23):12018-24
references.pmid: 22044358
references.citation: Bacteriol Rev. 1975 Jun;39(2):144-67
references.pmid: 1095004
references.citation: J Mol Biol. 1999 Jun 18;289(4):1017-28
references.pmid: 10369779
references.citation: Science. 2010 Oct 8;330(6001):201-4
references.pmid: 20724584
references.citation: Structure. 1997 Feb 15;5(2):203-16
references.pmid: 9032080
references.citation: Proc Natl Acad Sci U S A. 2005 Aug 2;102(31):10913-8
references.pmid: 16043709
references.citation: Front Microbiol. 2012 Oct 11;3:357
references.pmid: 23087678
references.citation: Appl Environ Microbiol. 2011 Nov;77(22):7962-74
references.pmid: 21948834
references.citation: Cell Mol Biol (Noisy-le-grand). 2004 Jul;50(5):553-61
references.pmid: 15559972
references.citation: Nat Rev Microbiol. 2003 Dec;1(3):200-8
references.pmid: 15035024
references.citation: Proc Natl Acad Sci U S A. 1987 Nov;84(21):7524-8
references.pmid: 3478708
references.citation: Biochemistry. 2008 Oct 14;47(41):10852-62
references.pmid: 18803407
references.citation: Biochemistry. 1997 Oct 14;36(41):12535-41
references.pmid: 9376358
references.citation: Appl Environ Microbiol. 2010 Oct;76(19):6412-22
references.pmid: 20675448
references.citation: Proteins. 1995 Mar;21(3):167-95
references.pmid: 7784423
references.citation: Environ Sci Technol. 2013 Oct 1;47(19):10860-7
references.pmid: 23937111
references.citation: Proc Natl Acad Sci U S A. 2012 Dec 11;109(50):20292-7
references.pmid: 21969552
references.citation: Methods Enzymol. 1986;131:389-433
references.pmid: 3773767
references.citation: Trends Microbiol. 2004 Dec;12(12):532-4
references.pmid: 15539111
references.citation: Environ Microbiol. 2012 Sep;14(9):2405-16
references.pmid: 22616650
references.citation: Biochemistry. 2002 Jan 29;41(4):1372-80
references.pmid: 11802740
references.citation: Environ Sci Technol. 2011 Aug 15;45(16):6709-15
references.pmid: 21699212