title: Graphene functionalized porous Au-paper based electrochemiluminescence device for detection of DNA using luminescent silver nanoparticles coated calcium carbonate/carboxymethyl chitosan hybrid microspheres as labels.
abstract: In the paper, a simple and sensitive electrochemiluminescence (ECL) DNA sensor based on graphene-modified porous Au-paper working electrode (GR/Au-PWE) and calcium carbonate/carboxymethyl chitosan (CaCO3/CMC) hybrid microspheres @ luminescent silver nanoparticles (AgNPs) composites was developed. The GR/Au-PWE with excellent conductivity was successfully prepared for the immobilization of capture probe. The CaCO3/CMC hybrid microspheres were prepared by the precipitation of calcium carbonate in an aqueous solution containing CMC. The AgNPs was synthesized by thermal reduction of silver ions in glycine matrix, taking advantage of the solid-state matrix to control the nucleation and migration of reduced silver atoms. The CaCO3/CMC@AgNPs composites exhibited 3.6 times higher ECL intensity than the pure AgNPs-labeled reporter DNA. Taking advantage of dual-amplification effects, the paper-based DNA sensor could detect the target DNA quantitatively, in the range of 4.0×10(-17)-5.0×10(-11) M, with a limit of detection as low as 8.5×10(-18) M, and perform excellent selectivity. The simple, low-cost, sensitive device could be easily applied for point-of-care testing, public health and environmental monitoring in remote regions, developing or developed countries.
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Meng
authors.initials: M
authors.lastname: Li
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Yanhu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China. Electronic address: ujn.yujh@gmail.com.
authors.forename: Jinghua
authors.initials: J
authors.lastname: Yu
authors.affiliation: Shandong Provincial Key Laboratory of Preparation and Measurement of Building Materials, University of Jinan, Jinan 250022, PR China.
authors.forename: Shenguang
authors.initials: S
authors.lastname: Ge
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Mei
authors.initials: M
authors.lastname: Yan
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation; D002119:Calcium Carbonate / Q000737:chemistry*; D048271:Chitosan / Q000031:analogs & derivatives* / Q000737:chemistry; D004247:DNA / Q000032:analysis* / Q000097:blood; D055664:Electrochemical Techniques / Q000295:instrumentation*; D004867:Equipment Design; D006046:Gold / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D008163:Luminescent Measurements / Q000295:instrumentation; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation; D008863:Microspheres; D008958:Models, Molecular; D053758:Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D010209:Paper; D016062:Porosity; D012834:Silver / Q000737:chemistry*
chemical_list: C514968:carboxymethyl-chitosan; D012834:Silver; D006046:Gold; D006108:Graphite; D004247:DNA; D048271:Chitosan; D002119:Calcium Carbonate
keywords: Calcium carbonate/carboxymethyl chitosan hybrid microspheres; DNA sensor; Electrochemiluminescence; Graphene-modified porous Au-paper electrode; Luminescent silver nanoparticles