title: Genetic engineering of industrial Saccharomyces cerevisiae strains using a selection/counter-selection approach.
abstract: Gene modification of laboratory yeast strains is currently a very straightforward task thanks to the availability of the entire yeast genome sequence and the high frequency with which yeast can incorporate exogenous DNA into its genome. Unfortunately, laboratory strains do not perform well in industrial settings, indicating the need for strategies to modify industrial strains to enable strain development for industrial applications. Here we describe approaches we have used to genetically modify industrial strains used in winemaking.
authors.affiliation: The Australian Wine Research Institute, 197, Glen Osmond, Adelaide, SA, 5064, Australia.
authors.forename: Dariusz R
authors.initials: DR
authors.lastname: Kutyna
authors.forename: Antonio G
authors.initials: AG
authors.lastname: Cordente
authors.forename: Cristian
authors.initials: C
authors.lastname: Varela
mesh_terms: D004171:Diploidy; D005818:Genetic Engineering / Q000379:methods*; D016678:Genome / Q000235:genetics; D006238:Haploidy; D007221:Industry; D011401:Promoter Regions, Genetic / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000502:physiology; D013172:Spores, Fungal; D014170:Transformation, Genetic