title: [Effect of alendronate-loaded bone cement on osteoblast].
abstract: OBJECTIVE
  To determine the effect of bone cement, with different amounts of alendronate on osteoblast, and determine the cytotoxicity of alendronate-integrated bone cement from the viewpoint of cell biology.
  
  
  METHODS
  According to different additions (0, 10, 50, 100, 500, 1 000 mg) of alendronate in 50 g Cemex®XL bone cement powder, the experiments were divided into 6 groups, namely G0-G5 groups. In all groups, the adhesive capacity of osteoblast-like cells MG-63 was evaluated by electron microscope, the optical density (OD) value of cells by MTT colorimetry method, the alkaline phosphatase activity (AKP) by AKP assay kit, the apoptosis rates by Annexin-V-FITC apoptosis detection kit, and the bone mineralization potentiality by phase contrast microscope.
  
  
  RESULTS
  The adhesive capacity of MG-63 was good in all groups. Compared with the G0 group, the cell apoptosis was inhibited in G1-G4 groups while in G5 group the cell apoptosis was promoted and cell proliferation was inhibited (P<0.05). In all groups, no significant difference was found in alkaline phosphatase activity and bone mineralization potentiality (P>0.05).
  
  
  CONCLUSIONS
  Less than 500 mg alendronate added in Cemex®XL 50 g bone cement powder has no cytotoxicity on osteoblasts.
authors.affiliation: Department of Orthopaedics, Second Xiangya Hospital, Central South University, Changsha 410011, China.
authors.forename: Deye
authors.initials: D
authors.lastname: Song
authors.forename: Jiangdong
authors.initials: J
authors.lastname: Ni
authors.forename: Xinzhan
authors.initials: X
authors.lastname: Mao
authors.forename: Muliang
authors.initials: M
authors.lastname: Ding
mesh_terms: D019386:Alendronate; D017209:Apoptosis; D001843:Bone Cements; D002448:Cell Adhesion; D049109:Cell Proliferation; D006801:Humans; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects*
chemical_list: D001843:Bone Cements; D019386:Alendronate