title: Sequence characterization and spatiotemporal expression patterns of PbS26-RNase gene in Chinese White Pear (Pyrus bretschneideri).
abstract: Many flowering plants exhibit an important intraspecific reproductive barrier phenomenon, that is, self-incompatibility (SI), in which S-RNase genes play a significant role. To clarify the specific function of S-RNase genes in Chinese pears, the full length cDNA of PbS 26 -RNase was isolated by rapid amplification of cDNA ends (RACE) technology from Chinese white pear (Pyrus bretschneideri) cultivar "Hongpisu." The cDNA sequence for PbS 26 -RNase was deposited in GenBank under accession number EU081888. At the amino acid level, the PbS 26 -RNase displayed the highest similarity (96.9%) with PcSa-RNase of P. communis, and only seven amino acid differences were present in the two S-RNases. Phylogenetic analysis of rosaceous S-RNases indicated that the PbS 26 -RNase clustered with maloideous S-RNases, forming a subfamily-specific not a species-specific group. The PbS 26 -RNase gene was specifically expressed in the style but not other tissues/organs. The expression level of the PbS 26 -RNase gene rapidly increased at bell balloon stage (BBS), and then it dropped after pollination. However, the abundance of the PbS 26 -RNase gene transcript in the style was greater after cross-pollination than after self-pollination. In addition, a method for rapidly detecting the PbS 26 -RNase gene was developed via allele-specific primers design. The present study could provide a scientific basis for fully clarifying the mechanism of pear SI at the molecular level.
authors.affiliation: Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha 410004, China ; Department of Plant Science and Landscape Architecture, University of Connecticut, Storrs, CT 06269, USA.
authors.forename: Lin
authors.identifier: 0000-0002-7412-5784
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha 410004, China.
authors.forename: Baoguang
authors.initials: B
authors.lastname: Jia
authors.affiliation: Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha 410004, China.
authors.forename: Feng
authors.identifier: 0000-0001-5179-2261
authors.initials: F
authors.lastname: Zou
authors.affiliation: Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha 410004, China.
authors.forename: Xiaofeng
authors.identifier: 0000-0002-4836-7261
authors.initials: X
authors.lastname: Tan
authors.affiliation: Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha 410004, China.
authors.forename: Min
authors.initials: M
authors.lastname: Liu
authors.affiliation: Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha 410004, China.
authors.forename: Zhibo
authors.initials: Z
authors.lastname: Song
authors.affiliation: Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha 410004, China.
authors.forename: Yanling
authors.initials: Y
authors.lastname: Zeng
authors.affiliation: Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha 410004, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Jiang
authors.affiliation: Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha 410004, China.
authors.forename: Deyi
authors.initials: D
authors.lastname: Yuan
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D035264:Flowers / Q000502:physiology*; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D008969:Molecular Sequence Data; D054817:Pollination / Q000235:genetics*; D031989:Pyrus / Q000145:classification / Q000502:physiology*; D012260:Ribonucleases / Q000737:chemistry* / Q000502:physiology*; D013045:Species Specificity; D014018:Tissue Distribution
chemical_list: D012260:Ribonucleases
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