title: Establishment and characterization of immortalized bovine endometrial epithelial cells.
abstract: Bovine primary uterine endometrial epithelial cells (EECs) are not ideal for long-term studies, because primary EECs lose hormone responsiveness quickly, and/or they tend to have a short life span. The aims of this study were to establish immortalized bovine EECs and to characterize these cells following long-term cultures. Immortalized bovine EECs were established by transfecting retroviral vectors encoding human papillomavirus (HPV) E6 and E7, and human telomerase reverse transcriptase (hTERT) genes. Established bovine immortalized EECs (imEECs) showed the same morphology as primary EECs, and could be grown without any apparent changes for over 60 passages. In addition, imEECs have maintained the features as EECs, exhibiting oxytocin (OT) and interferon tau (IFNT) responsiveness. Therefore, these imEECs, even after numbers of passages, could be used as an in vitro model to investigate cellular and molecular mechanisms, by which the uterine epithelium responds to IFNT stimulation, the event required for the maternal recognition of pregnancy in the bovine species.
authors.affiliation: Laboratory of Theriogenology and Animal Breeding, Graduate School of Agricultural and Life Science, the University of Tokyo, Tokyo.
authors.forename: Hanako
authors.initials: H
authors.lastname: Bai
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Sakurai
authors.forename: Rulan
authors.initials: R
authors.lastname: Bai
authors.forename: Sachiko
authors.initials: S
authors.lastname: Yamakoshi
authors.forename: Etsunari
authors.initials: E
authors.lastname: Aoki
authors.forename: Mariko
authors.initials: M
authors.lastname: Kuse
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Okuda
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Imakawa
mesh_terms: D000818:Animals; D002417:Cattle; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D003584:Cytological Techniques / Q000379:methods*; D015237:Dinoprost; D004717:Endometrium / Q000166:cytology* / Q000378:metabolism; D005260:Female; D005822:Genetic Vectors; D007370:Interferon Type I / Q000494:pharmacology; D007633:Keratins / Q000378:metabolism; D009856:Oncogene Proteins, Viral / Q000235:genetics; D010121:Oxytocin / Q000494:pharmacology; D050725:Papillomavirus E7 Proteins / Q000235:genetics; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000494:pharmacology; D012190:Retroviridae / Q000235:genetics; D013268:Stimulation, Chemical; D019098:Telomerase / Q000235:genetics; D013997:Time Factors; D014162:Transfection; D014746:Vimentin / Q000378:metabolism
chemical_list: C118860:E6 protein, Human papillomavirus type 6; D007370:Interferon Type I; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D011257:Pregnancy Proteins; D014746:Vimentin; C020947:interferon tau; D010121:Oxytocin; D007633:Keratins; D015237:Dinoprost; D019098:Telomerase
keywords: bovine; endometrial epithelial cell; immortalization
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