title: Immunotherapy reduces allergen-mediated CD66b expression and myeloperoxidase levels on human neutrophils from allergic patients.
abstract: CD66b is a member of the carcinoembryonic antigen family, which mediates the adhesion between neutrophils and to endothelial cells. Allergen-specific immunotherapy is widely used to treat allergic diseases, and the molecular mechanisms underlying this therapy are poorly understood. The present work was undertaken to analyze A) the in vitro effect of allergens and immunotherapy on cell-surface CD66b expression of neutrophils from patients with allergic asthma and rhinitis and B) the in vivo effect of immunotherapy on cell-surface CD66b expression of neutrophils from nasal lavage fluid during the spring season. Myeloperoxidase expression and activity was also analyzed in nasal lavage fluid as a general marker of neutrophil activation.
  
  
  RESULTS
  CD66b cell-surface expression is upregulated in vitro in response to allergens, and significantly reduced by immunotherapy (p<0.001). Myeloperoxidase activity in nasal lavage fluid was also significantly reduced by immunotherapy, as were the neutrophil cell-surface expression of CD66b and myeloperoxidase (p<0.001). Interestingly, CD66b expression was higher in neutrophils from nasal lavage fluid than those from peripheral blood, and immunotherapy reduced the number of CD66+MPO+ cells in nasal lavage fluid. Thus, immunotherapy positive effects might, at least in part, be mediated by the negative regulation of the CD66b and myeloperoxidase activity in human neutrophils.
authors.affiliation: Servicio Regional de Inmunología y Alergia, Hospital Universitario Virgen Macarena, Sevilla, Spain.
authors.forename: Rocio
authors.initials: R
authors.lastname: Aroca
authors.affiliation: Servicio Regional de Inmunología y Alergia, Hospital Universitario Virgen Macarena, Sevilla, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Chamorro
authors.affiliation: Servicio Regional de Inmunología y Alergia, Hospital Universitario Virgen Macarena, Sevilla, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Vega
authors.affiliation: Servicio Regional de Inmunología y Alergia, Hospital Universitario Virgen Macarena, Sevilla, Spain.
authors.forename: Inmaculada
authors.initials: I
authors.lastname: Ventura
authors.affiliation: Servicio Regional de Inmunología y Alergia, Hospital Universitario Virgen Macarena, Sevilla, Spain.
authors.forename: Elisa
authors.initials: E
authors.lastname: Gómez
authors.affiliation: Departamento de Medicina, Facultad de Medicina, Universidad de Sevilla, Sevilla, Spain.
authors.forename: Ramón
authors.initials: R
authors.lastname: Pérez-Cano
authors.affiliation: Research Laboratory, Carlos Haya Hospital-Fundacion IMABIS, Málaga, Spain.
authors.forename: Miguel
authors.initials: M
authors.lastname: Blanca
authors.affiliation: Servicio Regional de Inmunología y Alergia, Hospital Universitario Virgen Macarena, Sevilla, Spain; Departamento de Medicina, Facultad de Medicina, Universidad de Sevilla, Sevilla, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Monteseirín
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000276:immunology* / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000276:immunology* / Q000378:metabolism; D002478:Cells, Cultured; D003888:Desensitization, Immunologic / Q000379:methods; D005260:Female; D005434:Flow Cytometry; D058851:GPI-Linked Proteins / Q000276:immunology / Q000378:metabolism; D006801:Humans; D006967:Hypersensitivity / Q000097:blood / Q000276:immunology* / Q000628:therapy; D007073:Immunoglobulin E / Q000276:immunology / Q000378:metabolism; D007074:Immunoglobulin G / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology; D008297:Male; D017817:Nasal Lavage Fluid / Q000276:immunology; D009504:Neutrophils / Q000276:immunology* / Q000378:metabolism; D009195:Peroxidase / Q000276:immunology* / Q000378:metabolism; D015854:Up-Regulation / Q000276:immunology
chemical_list: D015703:Antigens, CD; C504120:CEACAM8 protein, human; D015815:Cell Adhesion Molecules; D058851:GPI-Linked Proteins; D007074:Immunoglobulin G; D008070:Lipopolysaccharides; D007073:Immunoglobulin E; D009195:Peroxidase
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