title: MAPs/bFGF-PLGA microsphere composite-coated titanium surfaces promote increased adhesion and proliferation of fibroblasts.
abstract: Infection and epithelial downgrowth are two major problems with maxillofacial transcutaneous implants, and both are mainly due to lack of stable closure of soft tissues at transcutaneous sites. Fibroblasts have been shown to play a key role in the formation of biological seals. In this work, titanium (Ti) model surfaces were coated with mussel adhesive proteins (MAPs) utilizing its unique adhesion ability on diverse inorganic and organic surfaces in wet environments. Prepared basic fibroblast growth factor (bFGF)-poly(lactic-co-glycolic acid) (PLGA) microspheres can be easily synthesized and combined onto MAPs-coated Ti surfaces, due to the negative surface charges of microspheres in aqueous solution, which is in contrast to the positive charges of MAPs. Titanium model surfaces were divided into three groups. Group A: MAPs/bFGF-PLGA microspheres composite-coated Ti surfaces. Group B: MAPs-coated Ti surfaces. Group C: uncoated Ti surfaces. The effects of coated Ti surfaces on adhesion of fibroblasts, cytoskeletal organization, proliferation, and extracellular matrix (ECM)-related gene expressions were examined. The results revealed increased adhesion (P < 0.05), enhanced actin cytoskeletal organization, and up-regulated ECM-related gene expressions in groups A and B compared with group C. Increased proliferation of fibroblasts during five days of incubation was observed in group A compared with groups B and C (P < 0.05). Collectively, the results from this in vitro study demonstrated that MAPs/bFGF-PLGA microspheres composite-coated Ti surfaces had the ability to increase fibroblast functionality. In addition, MAPs/bFGF-PLGA microsphere composite-coated Ti surfaces should be studied further as a method of promoting formation of stable biological seals around transcutaneous sites.
authors.affiliation: Department of Prosthetics, School of Stomatology, The Fourth Military Medical University, Xi'an, People's Republic of China.
authors.forename: Zhongshan
authors.initials: Z
authors.lastname: Wang
authors.forename: Guofeng
authors.initials: G
authors.lastname: Wu
authors.forename: Shizhu
authors.initials: S
authors.lastname: Bai
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Feng
authors.forename: Yan
authors.initials: Y
authors.lastname: Dong
authors.forename: Jian
authors.initials: J
authors.lastname: Zhou
authors.forename: Haiyan
authors.initials: H
authors.lastname: Qin
authors.forename: Yimin
authors.initials: Y
authors.lastname: Zhao
mesh_terms: D000818:Animals; D049872:Bivalvia / Q000737:chemistry; D002214:Capsules / Q000008:administration & dosage / Q000138:chemical synthesis; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis / Q000494:pharmacology; D003188:Composite Resins / Q000494:pharmacology; D016222:Fibroblast Growth Factor 2 / Q000737:chemistry / Q000494:pharmacology*; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry / Q000494:pharmacology*; D008863:Microspheres; D009934:Organization and Administration; D011100:Polyglycolic Acid / Q000737:chemistry / Q000494:pharmacology*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011506:Proteins / Q000737:chemistry / Q000494:pharmacology*; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D002214:Capsules; D020099:Coated Materials, Biocompatible; D003188:Composite Resins; D011506:Proteins; C037403:adhesive protein, mussel; D016222:Fibroblast Growth Factor 2; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D014025:Titanium