title: Analysis on bilateral hindlimb mapping in motor cortex of the rat by an intracortical microstimulation method.
abstract: Intracortical microstimulation (ICMS) is a technique that was developed to derive movement representation of the motor cortex. Although rats are now commonly used in motor mapping studies, the precise characteristics of rat motor map, including symmetry and consistency across animals, and the possibility of repeated stimulation have not yet been established. We performed bilateral hindlimb mapping of motor cortex in six Sprague-Dawley rats using ICMS. ICMS was applied to the left and the right cerebral hemisphere at 0.3 mm intervals vertically and horizontally from the bregma, and any movement of the hindlimbs was noted. The majority (80%± 11%) of responses were not restricted to a single joint, which occurred simultaneously at two or three hindlimb joints. The size and shape of hindlimb motor cortex was variable among rats, but existed on the convex side of the cerebral hemisphere in all rats. The results did not show symmetry according to specific joints in each rats. Conclusively, the hindlimb representation in the rat motor cortex was conveniently mapped using ICMS, but the characteristics and inter-individual variability suggest that precise individual mapping is needed to clarify motor distribution in rats.
authors.affiliation: Department of Neurological Surgery, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.
authors.forename: Han Yu
authors.identifier: 0000-0002-3089-1057
authors.initials: HY
authors.lastname: Seong
authors.affiliation: Department of Neurosurgery, Wooridul Spine Hospital, Seoul, Korea.
authors.forename: Ji Young
authors.initials: JY
authors.lastname: Cho
authors.affiliation: Department of Neurosurgery, Haeundae Paik Hospital, Inje University College of Medicine, Busan, Korea.
authors.forename: Byeong Sam
authors.initials: BS
authors.lastname: Choi
authors.affiliation: Department of Neurological Surgery, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.
authors.forename: Joong Kee
authors.initials: JK
authors.lastname: Min
authors.affiliation: Department of Computer Science and Engineering, University of Notre Dame, Notre Dame, IN, USA.
authors.forename: Yong Hwan
authors.initials: YH
authors.lastname: Kim
authors.affiliation: Department of Neurological Surgery, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.
authors.forename: Sung Woo
authors.initials: SW
authors.lastname: Roh
authors.affiliation: Department of Neurological Surgery, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.
authors.forename: Jeong Hoon
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Department of Neurological Surgery, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.
authors.forename: Sang Ryong
authors.identifier: 0000-0002-8340-7978
authors.initials: SR
authors.lastname: Jeon
mesh_terms: D000818:Animals; D001931:Brain Mapping; D004558:Electric Stimulation; D004566:Electrodes; D006614:Hindlimb / Q000502:physiology*; D008297:Male; D009044:Motor Cortex / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley
keywords: Brain Mapping; Deep Brain Stimulation; Hindlimb; Motor Cortex
references.citation: Behav Brain Res. 2009 Aug 24;202(1):138-41
references.pmid: 19447290
references.citation: J Neurosci Methods. 2008 Jul 30;172(2):255-62
references.pmid: 18582949
references.citation: Exp Brain Res. 1972;14(3):243-56
references.pmid: 4626360
references.citation: Cereb Cortex. 2011 Apr;21(4):865-76
references.pmid: 20739477
references.citation: Alcohol. 2010 Mar;44(2):185-94
references.pmid: 20083368
references.citation: J Neurosci Methods. 2007 Mar 30;161(1):118-25
references.pmid: 17178423
references.citation: J Neurophysiol. 1998 Dec;80(6):3321-5
references.pmid: 9862925
references.citation: Exp Neurol. 2009 Jun;217(2):421-8
references.pmid: 19361501
references.citation: Brain Res. 1986 Mar;396(1):77-96
references.pmid: 3708387
references.citation: Brain Res. 1985 Sep 30;344(1):49-61
references.pmid: 4041868
references.citation: J Neurosurg Spine. 2013 Aug;19(2):248-55
references.pmid: 23725395
references.citation: Neurosci Lett. 2003 Jun 5;343(2):121-4
references.pmid: 12759179
references.citation: J Neurosci Methods. 2011 Mar 15;196(1):60-9
references.pmid: 21219927
references.citation: J Comp Neurol. 2004 Nov 22;479(4):360-73
references.pmid: 15514982
references.citation: Brain Res. 1971 Mar 19;27(1):97-109
references.pmid: 4251989
references.citation: J Comp Neurol. 1982 Nov 20;212(1):76-88
references.pmid: 6294151
references.citation: Brain Res. 1999 Jul 24;835(2):175-87
references.pmid: 10415372
references.citation: Cereb Cortex. 1994 Mar-Apr;4(2):205-14
references.pmid: 8038569
references.citation: Eur J Neurosci. 2008 Sep;28(5):951-63
references.pmid: 18717732
references.citation: Brain Res Bull. 2002 Nov 30;59(3):197-203
references.pmid: 12431749