title: New screening method to detect carriage of carbapenemase-producing Enterobacteriaceae in patients within 24 hours.
abstract: Rapid identification of patients colonized with carbapenemase-producing Enterobacteriaceae (CPE) is essential to prevent introduction and the spread of CPE in the hospital. This article presents the results of a new screening method to detect patients colonized with CPE within 24h after hospital admission. From high-risk patients rectal and throat swabs were collected and incubated overnight, after which DNA was isolated and tested for the most prevalent CPE genes (KPC, NDM, OXA-48, VIM and IMP) by a ligation-mediated real-time polymerase chain reaction. In 14 months 454 patients were screened; in six patients CPE were detected (carriage rate 1.3%).
authors.affiliation: Department of Medical Microbiology and Medical Immunology, Rijnstate, Velp, The Netherlands; Department of Medical Microbiology, Radboud University Medical Center, Nijmegen, The Netherlands. Electronic address: n.hanemaaijer@mmb.umcn.nl.
authors.forename: N M
authors.initials: NM
authors.lastname: Hanemaaijer
authors.affiliation: Department of Medical Microbiology and Medical Immunology, Rijnstate, Velp, The Netherlands.
authors.forename: R H T
authors.initials: RH
authors.lastname: Nijhuis
authors.affiliation: Department of Medical Microbiology and Medical Immunology, Rijnstate, Velp, The Netherlands.
authors.forename: B J
authors.initials: BJ
authors.lastname: Slotboom
authors.affiliation: Department of Medical Microbiology and Medical Immunology, Rijnstate, Velp, The Netherlands.
authors.forename: E M
authors.initials: EM
authors.lastname: Mascini
authors.affiliation: Department of Medical Microbiology and Medical Immunology, Rijnstate, Velp, The Netherlands.
authors.forename: A A
authors.initials: AA
authors.lastname: van Zwet
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001426:Bacterial Proteins / Q000235:genetics*; D001431:Bacteriological Techniques / Q000379:methods*; D002353:Carrier State / Q000175:diagnosis / Q000382:microbiology; D004755:Enterobacteriaceae / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D004756:Enterobacteriaceae Infections / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D008297:Male; D008403:Mass Screening / Q000379:methods*; D008875:Middle Aged; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D010614:Pharynx / Q000382:microbiology; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012007:Rectum / Q000382:microbiology; D013997:Time Factors; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D001618:beta-Lactamases; C063912:carbapenemase
keywords: Active surveillance; Carbapenemase-producing Enterobacteriaceae; Carbapenemases; Real-time polymerase chain reaction; Screening