title: ANRIL inhibits p15(INK4b) through the TGFβ1 signaling pathway in human esophageal squamous cell carcinoma.
abstract: The INK4b-ARF-INK4a gene cluster encodes three tumor suppressors: p15(INK4b), p14(ARF), and p16(INK4a). Antisense non-coding RNA in the INK4 locus (ANRIL) is transcribed in the opposite direction from this gene cluster. Recent studies suggest that ANRIL represses the expression of p15(INK4b), p14(ARF), and p16(INK4a); however, the underlying mechanism is unclear. In this study, the expressions of ANRIL in human esophageal squamous cell carcinoma (ESCC) tissues and matched adjacent non-tumor tissues were examined by quantitative real-time polymerase chain reaction. Compared with matched adjacent non-tumor tissues, the expression levels of ANRIL in ESCC tissues were significantly increased. Furthermore, inhibition of ANRIL was found to increase the expression of p15(INK4b) and transforming growth factor β1 (TGFβ1) and depletion of ANRIL in ESCC cell lines may inhibit cellular proliferation. Thus, our findings suggest a significant role of ANRIL in the occurrence and development of ESCC through TGFβ1 signaling pathways.
authors.affiliation: Institute of Oncology, The Affiliated Hospital of Jiangsu University, Zhenjiang 212001, China.
authors.forename: Deyu
authors.initials: D
authors.lastname: Chen
authors.affiliation: School of Clinical Medicine, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Zhaoyue
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Institute of Oncology, The Affiliated Hospital of Jiangsu University, Zhenjiang 212001, China. Electronic address: jq1001@ujs.edu.cn.
authors.forename: Chaoming
authors.initials: C
authors.lastname: Mao
authors.affiliation: School of Clinical Medicine, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Yuepeng
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Thoracic Surgery, The Affiliated Hospital of Jiangsu University, Zhenjiang 212001, China.
authors.forename: Lichao
authors.initials: L
authors.lastname: Yu
authors.affiliation: Institute of Oncology, The Affiliated Hospital of Jiangsu University, Zhenjiang 212001, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Yin
authors.affiliation: Institute of Oncology, The Affiliated Hospital of Jiangsu University, Zhenjiang 212001, China.
authors.forename: Shi
authors.initials: S
authors.lastname: Wu
authors.affiliation: Institute of Oncology, The Affiliated Hospital of Jiangsu University, Zhenjiang 212001, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Mou
authors.affiliation: School of Clinical Medicine, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhu
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D050762:Cyclin-Dependent Kinase Inhibitor p15 / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics*; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000378:metabolism; D000077277:Esophageal Squamous Cell Carcinoma; D006801:Humans; D062085:RNA, Long Noncoding / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics*; D015398:Signal Transduction / Q000235:genetics; D051785:Smad Proteins / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000096:biosynthesis / Q000235:genetics*; D025521:Tumor Suppressor Proteins / Q000235:genetics
chemical_list: C561518:CDKN2B antisense RNA, human; D050762:Cyclin-Dependent Kinase Inhibitor p15; D062085:RNA, Long Noncoding; D051785:Smad Proteins; D053773:Transforming Growth Factor beta1; D025521:Tumor Suppressor Proteins
keywords: Antisense non-coding RNA in the INK4 locus; Esophageal squamous cell carcinoma; Long non-coding RNA; Transforming growth factor β1; p15(INK4b)