title: Radiation-induced changes in serum lipidome of head and neck cancer patients.
abstract: Cancer radiotherapy (RT) induces response of the whole patient's body that could be detected at the blood level. We aimed to identify changes induced in serum lipidome during RT and characterize their association with doses and volumes of irradiated tissue. Sixty-six patients treated with conformal RT because of head and neck cancer were enrolled in the study. Blood samples were collected before, during and about one month after the end of RT. Lipid extracts were analyzed using MALDI-oa-ToF mass spectrometry in positive ionization mode. The major changes were observed when pre-treatment and within-treatment samples were compared. Levels of several identified phosphatidylcholines, including (PC34), (PC36) and (PC38) variants, and lysophosphatidylcholines, including (LPC16) and (LPC18) variants, were first significantly decreased and then increased in post-treatment samples. Intensities of changes were correlated with doses of radiation received by patients. Of note, such correlations were more frequent when low-to-medium doses of radiation delivered during conformal RT to large volumes of normal tissues were analyzed. Additionally, some radiation-induced changes in serum lipidome were associated with toxicity of the treatment. Obtained results indicated the involvement of choline-related signaling and potential biological importance of exposure to clinically low/medium doses of radiation in patient's body response to radiation.
authors.affiliation: Center for Translational Research and Molecular Biology of Cancer, Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice Branch, Wybrzeze Armii Krajowej 15, 44-100 Gliwice, Poland. kjelonek@io.gliwice.pl.
authors.forename: Karol
authors.initials: K
authors.lastname: Jelonek
authors.affiliation: Center for Translational Research and Molecular Biology of Cancer, Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice Branch, Wybrzeze Armii Krajowej 15, 44-100 Gliwice, Poland. m_pietrowska@io.gliwice.pl.
authors.forename: Monika
authors.initials: M
authors.lastname: Pietrowska
authors.affiliation: Center for Translational Research and Molecular Biology of Cancer, Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice Branch, Wybrzeze Armii Krajowej 15, 44-100 Gliwice, Poland. ros.malgorzata@gmail.com.
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Ros
authors.affiliation: Institute of Mathematics and Computer Science, Wroclaw University of Technology, Janiszewskiego 14a, 50-370 Wroclaw, Poland. Adam.Zagdanski@pwr.wroc.pl.
authors.forename: Adam
authors.initials: A
authors.lastname: Zagdanski
authors.affiliation: Institute of Biomedical Engineering and Instrumentation, Wroclaw University of Technology, Wybrzeze Wyspianskiego 27, 50-370 Wroclaw, Poland. agnieszka.suchwalko@pwr.wroc.pl.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Suchwalko
authors.affiliation: Faculty of Automatic Control, Electronics and Computer Science, Silesian University of Technology, Akademicka 16, 44-100 Gliwice, Poland. Joanna.Polanska@polsl.pl.
authors.forename: Joanna
authors.initials: J
authors.lastname: Polanska
authors.affiliation: Faculty of Automatic Control, Electronics and Computer Science, Silesian University of Technology, Akademicka 16, 44-100 Gliwice, Poland. Michal.Marczyk@polsl.pl.
authors.forename: Michal
authors.initials: M
authors.lastname: Marczyk
authors.affiliation: Center for Translational Research and Molecular Biology of Cancer, Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice Branch, Wybrzeze Armii Krajowej 15, 44-100 Gliwice, Poland. tomr22@tlen.pl.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Rutkowski
authors.affiliation: Center for Translational Research and Molecular Biology of Cancer, Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice Branch, Wybrzeze Armii Krajowej 15, 44-100 Gliwice, Poland. skladowski@io.gliwice.pl.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Skladowski
authors.affiliation: Biomedical Research Centre, Sheffield Hallam University, Sheffield S1 1WB, UK. M.R.Clench@shu.ac.uk.
authors.forename: Malcolm R
authors.initials: MR
authors.lastname: Clench
authors.affiliation: Center for Translational Research and Molecular Biology of Cancer, Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice Branch, Wybrzeze Armii Krajowej 15, 44-100 Gliwice, Poland. widlak@io.gliwice.pl.
authors.forename: Piotr
authors.initials: P
authors.lastname: Widlak
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D016000:Cluster Analysis; D006258:Head and Neck Neoplasms / Q000097:blood / Q000532:radiotherapy*; D006801:Humans; D008055:Lipids / Q000097:blood*; D008244:Lysophosphatidylcholines / Q000097:blood; D008297:Male; D008875:Middle Aged; D010713:Phosphatidylcholines / Q000097:blood; D011879:Radiotherapy Dosage; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013997:Time Factors
chemical_list: D008055:Lipids; D008244:Lysophosphatidylcholines; D010713:Phosphatidylcholines
references.citation: Cancer Res. 2006 Feb 1;66(3):1844-50
references.pmid: 16452246
references.citation: Head Neck. 2007 Apr;29(4):387-400
references.pmid: 16358297
references.citation: Proc Natl Acad Sci U S A. 2009 Feb 17;106(7):2089-90
references.pmid: 19211786
references.citation: Prog Lipid Res. 2010 Oct;49(4):450-75
references.pmid: 20643161
references.citation: J Cell Biochem. 2003 Oct 15;90(3):525-33
references.pmid: 14523987
references.citation: Radiat Res. 2000 Sep;154(3):342-6
references.pmid: 11012342
references.citation: Br J Cancer. 2001 Apr 20;84(8):1016-22
references.pmid: 11308247
references.citation: Lipids Health Dis. 2007 Jul 10;6:17
references.pmid: 17623088
references.citation: Nature. 1993 Jan 28;361(6410):315-25
references.pmid: 8381210
references.citation: Int J Radiat Oncol Biol Phys. 2006 Nov 1;66(3):706-13
references.pmid: 17011446
references.citation: Biomed Chromatogr. 2009 Oct;23(10):1079-85
references.pmid: 19382245
references.citation: Leukemia. 2010 Apr;24(4):679-86
references.pmid: 20130602
references.citation: J Lipid Res. 2010 Nov;51(11):3299-305
references.pmid: 20671299
references.citation: Bioinformatics. 2005 Aug 1;21(15):3201-12
references.pmid: 15914541
references.citation: Curr Med Chem. 2003 Oct;10(20):2123-45
references.pmid: 12871089
references.citation: Clin Exp Immunol. 1999 May;116(2):326-31
references.pmid: 10337026
references.citation: Int J Radiat Oncol Biol Phys. 2009 Feb 1;73(2):384-90
references.pmid: 18374510
references.citation: Radiat Res. 1998 Jul;150(1):43-51
references.pmid: 9650601
references.citation: Mutagenesis. 2003 May;18(3):265-71
references.pmid: 12714692
references.citation: J Biol Chem. 1957 May;226(1):497-509
references.pmid: 13428781
references.citation: Mass Spectrom Rev. 2006 May-Jun;25(3):409-49
references.pmid: 16463283
references.citation: J Cell Biol. 2000 Jul 10;150(1):F5-7
references.pmid: 10893276
references.citation: Radiat Res. 2003 Mar;159(3):312-9
references.pmid: 12600233
references.citation: Biochim Biophys Acta. 2002 May 23;1582(1-3):257-64
references.pmid: 12069836
references.citation: Semin Radiat Oncol. 2012 Jan;22(1):40-9
references.pmid: 22177877
references.citation: J Lipid Res. 2009 Apr;50 Suppl:S91-6
references.pmid: 19017611
references.citation: Nucleic Acids Res. 2007 Jan;35(Database issue):D527-32
references.pmid: 17098933
references.citation: Biochem Cell Biol. 2004 Feb;82(1):18-26
references.pmid: 15052325
references.citation: Bioinformatics. 2008 Oct 1;24(19):2129-36
references.pmid: 18694894
references.citation: J Lipid Res. 1993 Dec;34(12):2017-31
references.pmid: 8301223
references.citation: J Pharm Bioallied Sci. 2010 Jul;2(3):189-96
references.pmid: 21829314
references.citation: J Radiat Res. 2011;52(5):575-81
references.pmid: 21768750
references.citation: J Transl Med. 2013 Dec 05;11:299
references.pmid: 24304975
references.citation: BMC Bioinformatics. 2009 Jan 06;10:4
references.pmid: 19126200