title: Phenotypic and molecular characterization of multidrug resistant Klebsiella pneumoniae isolated from a university teaching hospital, China.
abstract: The multidrug-resistant rate of Klebsiella pneumoniae has risen rapidly worldwide. To better understand the multidrug resistance situation and molecular characterization of Klebsiella pneumoniae, a total of 153 Klebsiella pneumoniae isolates were collected, and drug susceptibility test was performed to detect its susceptibility patterns to 13 kinds of antibiotics. Phenotypic tests for carbapenemases ESBLs and AmpC enzyme-producing strains were performed to detect the resistance phenotype of the isolates. Then PCR amplification and sequencing analysis were performed for the drug resistance determinants. The results showed that 63 strains harbored bla CTX-M gene, and 14 strains harbored bla DHA gene. Moreover, there were 5 strains carrying bla KPC gene, among which 4 strains carried bla CTX-M, bla DHA and bla KPC genes, and these 4 strains were also resistant to imipenem. Our data indicated that drug-resistant Klebsiella pneumoniae were highly prevalent in the hospital. Thus it is warranted that surveillance of epidemiology of those resistant isolates should be a cause for concern, and appropriate drugs should be chosen.
authors.affiliation: Department of Clinical Laboratory, Shenzhen Shajing Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
authors.forename: Jikun
authors.initials: J
authors.lastname: Du
authors.affiliation: Department of Clinical Laboratory, Shenzhen Shajing Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
authors.forename: Peipei
authors.initials: P
authors.lastname: Li
authors.affiliation: Department of Clinical Laboratory, Shenzhen Shajing Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
authors.forename: Helu
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Clinical Laboratory, Shenzhen Shajing Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
authors.forename: Dongyue
authors.initials: D
authors.lastname: Lü
authors.affiliation: Department of Clinical Laboratory, Shenzhen Shajing Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Liang
authors.affiliation: Department of Clinical Laboratory, Shenzhen Shajing Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Dou
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D015373:Bacterial Typing Techniques; D002681:China / Q000453:epidemiology; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D062665:Epidemiological Monitoring; D015870:Gene Expression; D005838:Genotype; D006784:Hospitals, Teaching; D006785:Hospitals, University; D006801:Humans; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D007710:Klebsiella Infections / Q000188:drug therapy* / Q000453:epidemiology / Q000382:microbiology; D007711:Klebsiella pneumoniae / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D010641:Phenotype; D010957:Plasmids / Q000378:metabolism; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D007527:Isoenzymes; D001618:beta-Lactamases; C063912:carbapenemase
references.citation: Antimicrob Agents Chemother. 2006 Oct;50(10):3396-406
references.pmid: 17005822
references.citation: J Clin Microbiol. 2002 Jun;40(6):2153-62
references.pmid: 12037080
references.citation: Antimicrob Agents Chemother. 2008 Apr;52(4):1413-8
references.pmid: 18227191
references.citation: BMC Microbiol. 2012 Sep 11;12:201
references.pmid: 22967317
references.citation: Diagn Microbiol Infect Dis. 2011 May;70(1):119-23
references.pmid: 21398074
references.citation: Environ Microbiol. 2004 Jun;6(6):584-90
references.pmid: 15142246
references.citation: Antimicrob Agents Chemother. 2000 Mar;44(3):622-32
references.pmid: 10681329
references.citation: PLoS One. 2013;8(3):e59920
references.pmid: 23555831
references.citation: Antimicrob Agents Chemother. 2004 Jan;48(1):15-22
references.pmid: 14693513
references.citation: BMC Infect Dis. 2013 Feb 11;13:80
references.pmid: 23398691
references.citation: J Antimicrob Chemother. 2003 Feb;51(2):415-8
references.pmid: 12562713
references.citation: Int J Antimicrob Agents. 2012 Jun;39(6):514-7
references.pmid: 22481058
references.citation: Microb Drug Resist. 2010 Dec;16(4):317-25
references.pmid: 20524901
references.citation: J Hosp Infect. 2003 Mar;53(3):198-206
references.pmid: 12623321
references.citation: J Antimicrob Chemother. 2011 Feb;66(2):313-8
references.pmid: 21131694
references.citation: Curr Microbiol. 2011 May;62(5):1610-6
references.pmid: 21359845
references.citation: J Antimicrob Chemother. 2009 Nov;64(5):960-4
references.pmid: 19749204
references.citation: Clin Infect Dis. 2006 Aug 1;43(3):e26-8
references.pmid: 16804837
references.citation: Clin Microbiol Rev. 2007 Jul;20(3):440-58, table of contents
references.pmid: 17630334
references.citation: Antimicrob Agents Chemother. 2001 Feb;45(2):616-20
references.pmid: 11158766
references.citation: Antimicrob Agents Chemother. 2009 May;53(5):1998-2004
references.pmid: 19258268
references.citation: J Med Microbiol. 2009 Jun;58(Pt 6):774-778
references.pmid: 19429754
references.citation: J Clin Microbiol. 2003 Feb;41(2):772-7
references.pmid: 12574281
references.citation: J Clin Diagn Res. 2013 Sep;7(9):1975-8
references.pmid: 24179913
references.citation: Clin Microbiol Rev. 1998 Oct;11(4):589-603
references.pmid: 9767057
references.citation: Emerg Infect Dis. 2008 Feb;14(2):195-200
references.pmid: 18258110
references.citation: Antimicrob Agents Chemother. 2011 Sep;55(9):4058-63
references.pmid: 21746945
references.citation: Antimicrob Agents Chemother. 2010 Dec;54(12):5099-104
references.pmid: 20837757
references.citation: Emerg Infect Dis. 2013 Jan;19(1):133-6
references.pmid: 23260464
references.citation: J Antimicrob Chemother. 2007 Feb;59(2):321-2
references.pmid: 17185300
references.citation: Antimicrob Agents Chemother. 2005 Mar;49(3):1150-6
references.pmid: 15728917
references.citation: Pathol Biol (Paris). 2008 Jul;56(5):319-25
references.pmid: 18585867
references.citation: Kansenshogaku Zasshi. 2000 Jan;74(1):24-9
references.pmid: 10695291
references.citation: Int J Environ Res Public Health. 2013 Feb 26;10(3):808-15
references.pmid: 23442560
references.citation: PLoS One. 2013 Apr 29;8(4):e62774
references.pmid: 23658651