title: [Differentiation of human bone marrow mesenchymal stem cells into Leydig or steroidogenic cells in vivo].
abstract: OBJECTIVE
  To study the differentiation of human bone marrow mesenchymal stem cells (BMSCs) into Leydig or steroidogenic cells in vivo and the immunoreaction related to transplantation into mouse testis.
  
  
  METHODS
  After differentiation and cultivation, the 3rd-passage BMSCs were collected and labeled with Hoechest 33342, and joined the saline fluorouracil to form cell-suspending fluid. After injection of the etgane dimethane sulphonate (EDS), the mice received the transplanted cell-suspending fluid by testis net injection with a dose of each side testicular 0.05 mL. Since the first day prior to transplantation, mice were executed every 2 d (one mouse each time) and the testosterone concentrations were analyzed. The control group included 20 BALB/c mice without any treatment during the same period. The results were analyzed by microscopic observation, using 3beta-hydroxysteroid dehydrogenase (3beta-HSD) monoclonal antibody and mouse anti-human cell nucleus monoclonal antibody for immunofluorescence assay on the testis. For tracking the BMSCs, the cells which were positively stained with both 3beta-HSD and mouse anti-human cell nucleus monoclonal antibodies were retrieved.
  
  
  RESULTS
  Certain killing effect of EDS to the mouse Leydig cells was observed. Transplantation of human BMSCs into the mouse testis by testis net injection was effective and feasible, no immunoreactions were detected. After transplantation, no positive cells of 3beta-HSD) and mouse anti-human cell nucleus monoclonal antibody were found.
  
  
  CONCLUSIONS
  Transplantation of human BMSCs into the mouse testis by testis net injection was effective and feasible, no immunoreactions were detected. After transplantation, the human BMSCs failed to differentiate into Leydig cells or steroidogenic cells.
authors.forename: Shang-Qing
authors.initials: SQ
authors.lastname: Ren
authors.forename: Shi
authors.initials: S
authors.lastname: Deng
authors.forename: Yun-Jian
authors.initials: YJ
authors.lastname: Wu
authors.forename: Lin
authors.initials: L
authors.lastname: Hou
authors.forename: Tian
authors.initials: T
authors.lastname: Dong
authors.forename: Qiang
authors.initials: Q
authors.lastname: Dong
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D006801:Humans; D007985:Leydig Cells / Q000166:cytology*; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D008807:Mice, Inbred BALB C; D013737:Testis / Q000166:cytology*; D013739:Testosterone / Q000032:analysis
chemical_list: D013739:Testosterone