title: Label-free blood plasma test based on surface-enhanced Raman scattering for tumor stages detection in nasopharyngeal cancer.
abstract: This study aims to evaluate the feasibility of a label-free nanobiosensor based on blood plasma surface-enhanced Raman spectroscopy (SERS) method for exploring variability of different tumor (T) stages in nasopharyngeal cancer (NPC). Au nanoparticles as the SERS-active nanostructures were directly mixed with human blood plasma to enhance the Raman scattering signals. High quality SERS spectra can be acquired from blood plasma samples belong to 60 healthy volunteers, 25 NPC patients with T1 stage and 75 NPC patients with T2-T4 stage. A diagnostic accuracy of 83.5% and 93.3%, respectively, can be achieved for classification between early T (T1) stage cancer and normal; and advanced T (T2-T4) stage cancer and normal blood groups. This exploratory study demonstrates that the nanobiosensor based on SERS technique in conjunction with PCA-LDA has great potential as a clinical complement for different T stages detection in nasopharyngeal cancer.
authors.affiliation: 1] Key Laboratory of OptoElectronic Science and Technology for Medicine, Ministry of Education, Fujian Normal University, Fuzhou 350007, China [2] College of Integrated Traditional Chinese and Western Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian, 350122, China [3].
authors.forename: Duo
authors.initials: D
authors.lastname: Lin
authors.affiliation: 1] Fujian Provincial Cancer Hospital, Fuzhou 350001, China [2].
authors.forename: Jianji
authors.initials: J
authors.lastname: Pan
authors.affiliation: College of Integrated Traditional Chinese and Western Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian, 350122, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Huang
authors.affiliation: Key Laboratory of OptoElectronic Science and Technology for Medicine, Ministry of Education, Fujian Normal University, Fuzhou 350007, China.
authors.forename: Guannan
authors.initials: G
authors.lastname: Chen
authors.affiliation: Fujian Provincial Cancer Hospital, Fuzhou 350001, China.
authors.forename: Sufang
authors.initials: S
authors.lastname: Qiu
authors.affiliation: College of Integrated Traditional Chinese and Western Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian, 350122, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Shi
authors.affiliation: College of Integrated Traditional Chinese and Western Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian, 350122, China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Chen
authors.affiliation: College of Integrated Traditional Chinese and Western Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian, 350122, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Key Laboratory of OptoElectronic Science and Technology for Medicine, Ministry of Education, Fujian Normal University, Fuzhou 350007, China.
authors.forename: Shangyuan
authors.initials: S
authors.lastname: Feng
authors.affiliation: Key Laboratory of OptoElectronic Science and Technology for Medicine, Ministry of Education, Fujian Normal University, Fuzhou 350007, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Chen
mesh_terms: D016022:Case-Control Studies; D006046:Gold / Q000737:chemistry; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D009303:Nasopharyngeal Neoplasms / Q000097:blood* / Q000175:diagnosis / Q000473:pathology*; D009367:Neoplasm Staging; D011379:Prognosis; D012372:ROC Curve; D013059:Spectrum Analysis, Raman / Q000379:methods
chemical_list: D006046:Gold
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