title: The STAT3-binding long noncoding RNA lnc-DC controls human dendritic cell differentiation.
abstract: Long noncoding RNAs (lncRNAs) play important roles in diverse biological processes; however, few have been identified that regulate immune cell differentiation and function. Here, we identified lnc-DC, which was exclusively expressed in human conventional dendritic cells (DCs). Knockdown of lnc-DC impaired DC differentiation from human monocytes in vitro and from mouse bone marrow cells in vivo and reduced capacity of DCs to stimulate T cell activation. lnc-DC mediated these effects by activating the transcription factor STAT3 (signal transducer and activator of transcription 3). lnc-DC bound directly to STAT3 in the cytoplasm, which promoted STAT3 phosphorylation on tyrosine-705 by preventing STAT3 binding to and dephosphorylation by SHP1. Our work identifies a lncRNA that regulates DC differentiation and also broadens the known mechanisms of lncRNA action.
authors.affiliation: National Key Laboratory of Medical Immunology and Institute of Immunology, Second Military Medical University, Shanghai 200433, China.
authors.forename: Pin
authors.initials: P
authors.lastname: Wang
authors.forename: Yiquan
authors.initials: Y
authors.lastname: Xue
authors.forename: Yanmei
authors.initials: Y
authors.lastname: Han
authors.forename: Li
authors.initials: L
authors.lastname: Lin
authors.forename: Cong
authors.initials: C
authors.lastname: Wu
authors.forename: Sheng
authors.initials: S
authors.lastname: Xu
authors.forename: Zhengping
authors.initials: Z
authors.lastname: Jiang
authors.forename: Junfang
authors.initials: J
authors.lastname: Xu
authors.forename: Qiuyan
authors.initials: Q
authors.lastname: Liu
authors.forename: Xuetao
authors.initials: X
authors.lastname: Cao
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D002454:Cell Differentiation; D002843:Chromatin / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology* / Q000502:physiology; D044127:Epigenesis, Genetic; D005786:Gene Expression Regulation; D006657:Histones / Q000378:metabolism; D006801:Humans; D008213:Lymphocyte Activation; D051379:Mice; D009000:Monocytes / Q000166:cytology; D009690:Nucleic Acid Conformation; D010766:Phosphorylation; D053824:Protein Tyrosine Phosphatase, Non-Receptor Type 6 / Q000378:metabolism; D062085:RNA, Long Noncoding / Q000378:metabolism*; D050796:STAT3 Transcription Factor / Q000378:metabolism*; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D002843:Chromatin; D006657:Histones; D062085:RNA, Long Noncoding; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; D053824:Protein Tyrosine Phosphatase, Non-Receptor Type 6