title: Molecular imprinting and immobilization of cellulase onto magnetic Fe3O4@SiO2 nanoparticles.
abstract: Supermagnetic Fe3O4@SiO2 nanoparticles were molecular-imprinted prepared with cellulase as the template. The molecular imprinted nanoparticles were used as support to immobilization of cellulase. The transmission electron microscopy confirmed the core-shell structure and revealed that the size of the nanoparticles was around 10 nm. It was observed that cellulase was immobilized on the nanoparticles successfully from the Fourier transform infrared spectra. The adsorption of cellulase on the nanoparticles was specific and rapid. A high immobilization efficiency of 95% was achieved after the optimization. At 70 degrees C, the half-life of the immobilized cellulase was 3.3-fold of the free enzyme. Compared with the free enzyme, the immobilized cellulase has the same optimal pH, higher optimal temperature, better thermal stability and higher catalytic efficiency. The results strongly suggest that the immobilized cellulase on molecular imprinted Fe3O4@SiO2 has the potential applications in the production of bioethanol, paper and pulp industry, and pharmaceutical industry.
authors.forename: Yue
authors.initials: Y
authors.lastname: Li
authors.forename: Xiang-Yu
authors.initials: XY
authors.lastname: Wang
authors.forename: Rui-Zhuo
authors.initials: RZ
authors.lastname: Zhang
authors.forename: Xiao-Yun
authors.initials: XY
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Xi-Ming
authors.initials: XM
authors.lastname: Xu
authors.forename: Ye-Wang
authors.initials: YW
authors.lastname: Zhang
mesh_terms: D002480:Cellulase / Q000378:metabolism*; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D054802:Molecular Imprinting / Q000379:methods*; D009994:Osmolar Concentration; D012822:Silicon Dioxide / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature
chemical_list: D004800:Enzymes, Immobilized; D058185:Magnetite Nanoparticles; D012822:Silicon Dioxide; D002480:Cellulase