title: In vitro amphotericin B susceptibility of Malassezia pachydermatis determined by the CLSI broth microdilution method and Etest using lipid-enriched media.
abstract: We determined the in vitro amphotericin B susceptibility of 60 Malassezia pachydermatis isolates by the CLSI broth microdilution method and the Etest using lipid-enriched media. All isolates were susceptible at MICs of ≤ 1 μg/ml, confirming the high activity of amphotericin B against this yeast species. Overall, the essential agreement between the tested methods was high (80% and 96.7% after 48 h and 72 h, respectively), and all discrepancies were regarded as nonsubstantial.
authors.affiliation: Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, Spain.
authors.forename: Sergio
authors.initials: S
authors.lastname: Álvarez-Pérez
authors.affiliation: Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, Spain Hospital Clínico Veterinario, Universidad Complutense, Madrid, Spain jlblanco@ucm.es.
authors.forename: José L
authors.initials: JL
authors.lastname: Blanco
authors.affiliation: Servicio de Microbiología Clínica y Enfermedades Infecciosas, Hospital General Universitario Gregorio Marañón, Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain Departamento de Medicina, Facultad de Medicina, Universidad Complutense, Madrid, Spain.
authors.forename: Teresa
authors.initials: T
authors.lastname: Peláez
authors.affiliation: Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, Spain Hospital Clínico Veterinario, Universidad Complutense, Madrid, Spain.
authors.forename: Maite
authors.initials: M
authors.lastname: Cutuli
authors.affiliation: Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, Spain Hospital Clínico Veterinario, Universidad Complutense, Madrid, Spain.
authors.forename: Marta E
authors.initials: ME
authors.lastname: García
mesh_terms: D000666:Amphotericin B / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology*; D052978:Disk Diffusion Antimicrobial Tests; D008290:Malassezia / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data
chemical_list: D000935:Antifungal Agents; D000666:Amphotericin B
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