title: [Comparison between the centrifugation on MPA C10 (Roche Diagnostics) and the centrifugation according recommendations of GEHT (Groupe d'étude de l'hémostase et de la thrombose) for the daily hemostasis assays].
abstract: Actually, many laboratories tend to acquire pre analytic automates to prepare specimens for analysis. For haemostasis, these pre analytical modules are not always in agreement with the recommendations from the Groupe d'étude de l'hémostase et de la thrombose (GEHT). For example in the MPA C10 module (Roche Diagnostics) the speed of centrifugation was not rather fast compared with the GEHT recommandations. Then, to be able to use this automate for routine coagulation assays, we compared results of Quick time, activated partial prothombin time, fibrinogen, factor II, factor V, factor VII, factor X and antithrombin levels and unfractioned heparin anti-Xa activity measurement after MPA (1,885 g - 999 sec) or GEHT (2,500 g - 900 sec) protocol of centrifugation. First, we verified platelet counts: in 82% of specimens, the platelet counts were under 10.10(9)/L after centrifugation on MPA module. Moreover, a good correlation was observed in all comparisons. Then we concluded the MPA C10 module was usable for routine coagulation tests.
authors.affiliation: Unité fonctionnelle d'hémostase, hématologie biologique, Institut de biologie clinique, CHU de Rouen, France.
authors.forename: Fabrice
authors.initials: F
authors.lastname: Flamant
authors.affiliation: Unité fonctionnelle d'hémostase, hématologie biologique, Institut de biologie clinique, CHU de Rouen, France.
authors.forename: Jeanne-Yvonne
authors.initials: JY
authors.lastname: Borg
authors.affiliation: Unité fonctionnelle d'hémostase, hématologie biologique, Institut de biologie clinique, CHU de Rouen, France.
authors.forename: Bernard
authors.initials: B
authors.lastname: Lenormand
authors.affiliation: Unité fonctionnelle d'hémostase, hématologie biologique, Institut de biologie clinique, CHU de Rouen, France.
authors.forename: Véronique
authors.initials: V
authors.lastname: Le Cam-Duchez
mesh_terms: D000991:Antithrombins / Q000097:blood; D001780:Blood Coagulation Tests / Q000592:standards*; D001800:Blood Specimen Collection / Q000379:methods / Q000592:standards; D002498:Centrifugation / Q000592:standards; D005165:Factor V / Q000032:analysis; D005167:Factor VII / Q000032:analysis; D005340:Fibrinogen / Q000032:analysis; D006487:Hemostasis; D006801:Humans; D010976:Platelet Count; D017410:Practice Guidelines as Topic; D011516:Prothrombin / Q000032:analysis; D013927:Thrombosis / Q000097:blood* / Q000175:diagnosis*
chemical_list: D000991:Antithrombins; D005165:Factor V; D005167:Factor VII; D011516:Prothrombin; D005340:Fibrinogen
keywords: assays; centrifugation; haemostasis