title: Antitumor effects of a purified polysaccharide from Rhodiola rosea and its action mechanism.
abstract: In the last three decades, numerous polysaccharides and polysaccharide-protein complexes have been isolated from plant or animal and used as a promising source of therapeutic agents for cancer. In this study, we prepared a homogeneous polysaccharide (RRP-ws) from Rhodiola rosea and tested its immunomodulation and anti-cancer activity in vitro and in vivo experiments using Sarcoma 180 (S-180) cells. Preliminary physicochemical analysis identified that RRP-ws was composed of Glc, Gal, Man and Rha with a relative molar ratio of 4.2:2.4:1.6:1.0, and contained 95.14% of total carbohydrate, 2.08% of protein and no sulfate. In vitro experiment showed that RRP-ws exerted a direct cytotoxic effect on the growth of S-180 cells. In vivo experiment, RRP-ws could inhibit tumor growth of S-180 tumor transplanted in mice, and increase the relative spleen/thymus indexes and body weight. Furthermore, RRP-ws also increased the production of IL-2, TNF-α and IFN-γ in serum, and elevated the ratio of CD4+/CD8+ on peripheral blood T-lymphocyte in tumor bearing mice. The overall findings indicated that RRP-ws could be used as a novel promising immunotherapeutic agent in cancer treatment.
authors.affiliation: Tumor Center, First Hospital, Jilin University, Changchun 130021, China.
authors.forename: Zibin
authors.initials: Z
authors.lastname: Cai
authors.affiliation: Tumor Center, First Hospital, Jilin University, Changchun 130021, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Regeneration Medicine, School of Pharmaceutical Sciences, Jilin University, Changchun 130021, China.
authors.forename: Haotian
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Regeneration Medicine, School of Pharmaceutical Sciences, Jilin University, Changchun 130021, China.
authors.forename: Weiqun
authors.initials: W
authors.lastname: Yan
authors.affiliation: Department of Regeneration Medicine, School of Pharmaceutical Sciences, Jilin University, Changchun 130021, China.
authors.forename: Yulai
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Tumor Center, First Hospital, Jilin University, Changchun 130021, China.
authors.forename: Guanjun
authors.initials: G
authors.lastname: Wang
authors.affiliation: Tumor Center, First Hospital, Jilin University, Changchun 130021, China.
authors.forename: Jiuwei
authors.initials: J
authors.lastname: Cui
authors.affiliation: Department of Regeneration Medicine, School of Pharmaceutical Sciences, Jilin University, Changchun 130021, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000302:isolation & purification* / Q000627:therapeutic use; D045744:Cell Line, Tumor; D008297:Male; D051379:Mice; D009369:Neoplasms / Q000188:drug therapy / Q000473:pathology; D010936:Plant Extracts / Q000302:isolation & purification* / Q000627:therapeutic use; D011134:Polysaccharides / Q000302:isolation & purification* / Q000627:therapeutic use; D029771:Rhodiola; D016896:Treatment Outcome; D023041:Xenograft Model Antitumor Assays / Q000379:methods
chemical_list: D000970:Antineoplastic Agents; D010936:Plant Extracts; D011134:Polysaccharides