title: Simultaneous determination of cortisol and cortisone from human serum by liquid chromatography-tandem mass spectrometry.
abstract: A fast, sensitive, and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was validated and then the levels of cortisol and cortisone from sera of healthy adults were determined by the LC-MS/MS method. One hundred μ L of serum sample was directly extracted by adding 2 mL ethyl acetate, followed by chromatographic separation on a C18 column with a mobile phase consisting of 5 mM ammonium acetate and methanol (25 : 75, v/v). The precision, accuracy, and average recovery of the method were 1.5-5.3%, 95.4-102.5%, and 96.4% for cortisol, and 1.9-6.0%, 89.2-98.8%, and 79.9% for cortisone, respectively. The method was linear from 1.0 to 500.0 ng/mL (r(2) = 0.999) for cortisol and 2.5 to 100.0 ng/mL (r(2) = 0.998) for cortisone. The limits of detection (LOD) and quantification (LOQ) were 0.2 and 1.0 ng/mL for cortisol, and 1.0 and 2.5 ng/mL for cortisone, respectively. The average cortisol concentration (133.9 ± 63.7 ng/mL) of samples collected between 9:00 and 11:00 a.m. was higher approximately 4.4 times than that of cortisone (30.5 ± 10.7 ng/mL) (P < 0.0001). The average cortisone/cortisol ratio was 0.225. Therefore, the LC-MS/MS method may be useful for the diagnosis of some adrenal diseases and the assessment of 11 β -hydroxysteroid dehydrogenase (11 β -HSD) activity in clinical laboratories.
authors.affiliation: Department of Bioanalysis, Seoul Medical Science Institute & Seoul Clinical Laboratories, Seoul 152-766, Republic of Korea.
authors.forename: Sanghoo
authors.initials: S
authors.lastname: Lee
authors.affiliation: Department of Laboratory Medicine, Kwandong University College of Medicine, Gangneung 210-701, Republic of Korea.
authors.forename: Hwan-Sub
authors.initials: HS
authors.lastname: Lim
authors.affiliation: Department of Bioanalysis, Seoul Medical Science Institute & Seoul Clinical Laboratories, Seoul 152-766, Republic of Korea.
authors.forename: Hye-Jin
authors.initials: HJ
authors.lastname: Shin
authors.affiliation: Department of Bioanalysis, Seoul Medical Science Institute & Seoul Clinical Laboratories, Seoul 152-766, Republic of Korea.
authors.forename: Seol-A
authors.initials: SA
authors.lastname: Kim
authors.affiliation: Department of Bioanalysis, Seoul Medical Science Institute & Seoul Clinical Laboratories, Seoul 152-766, Republic of Korea.
authors.forename: Jimyeong
authors.initials: J
authors.lastname: Park
authors.affiliation: Department of Bioanalysis, Seoul Medical Science Institute & Seoul Clinical Laboratories, Seoul 152-766, Republic of Korea.
authors.forename: Hyun-Chul
authors.initials: HC
authors.lastname: Kim
authors.affiliation: Department of Bioanalysis, Seoul Medical Science Institute & Seoul Clinical Laboratories, Seoul 152-766, Republic of Korea.
authors.forename: Hyogyeong
authors.initials: H
authors.lastname: Kim
authors.affiliation: Department of Biological Engineering, College of Engineering, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Hyung Joo
authors.initials: HJ
authors.lastname: Kim
authors.affiliation: Department of Bioanalysis, Seoul Medical Science Institute & Seoul Clinical Laboratories, Seoul 152-766, Republic of Korea.
authors.forename: Yun-Tae
authors.initials: YT
authors.lastname: Kim
authors.affiliation: Department of Bioanalysis, Seoul Medical Science Institute & Seoul Clinical Laboratories, Seoul 152-766, Republic of Korea.
authors.forename: Kyoung-Ryul
authors.initials: KR
authors.lastname: Lee
authors.affiliation: Department of Bioanalysis, Seoul Medical Science Institute & Seoul Clinical Laboratories, Seoul 152-766, Republic of Korea.
authors.forename: Young-Jin
authors.identifier: 0000-0002-9980-8143
authors.initials: YJ
authors.lastname: Kim
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