title: Advances in whole-embryo imaging: a quantitative transition is underway.
abstract: With the advent of imaging probes and live microscopy, developmental biologists have markedly extended our understanding of the molecular and cellular details of embryonic development. To fully comprehend the complex mechanistic framework that forms the developing organism, quantitative studies with high fidelity in space and time are now required. We discuss how integrating established, newly introduced and future imaging tools with quantitative analysis will ensure that imaging can fulfil its promise to elucidate how new life begins.
authors.affiliation: Department of Biosystems Science and Engineering (D-BSSE), Eidgenössische Technische Hochschule (ETH) Zurich, 4058 Basel, Switzerland.
authors.forename: Periklis
authors.initials: P
authors.lastname: Pantazis
authors.affiliation: Laboratory for Optics and Biosciences, École Polytechnique, Centre National de la Recherche Scientifique (CNRS) UMR7645, and Institut National de la Santé et de la Recherche Médicale (INSERM) U696, 91120 Palaiseau, France.
authors.forename: Willy
authors.initials: W
authors.lastname: Supatto
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000033:anatomy & histology* / Q000196:embryology / Q000235:genetics; D004331:Drosophila melanogaster / Q000033:anatomy & histology* / Q000196:embryology / Q000235:genetics; D004625:Embryo, Nonmammalian; D047108:Embryonic Development; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics; D007091:Image Processing, Computer-Assisted / Q000295:instrumentation / Q000379:methods*; D036641:Microscopy, Fluorescence, Multiphoton; D057054:Molecular Imaging / Q000295:instrumentation / Q000379:methods*; D015335:Molecular Probes; D013050:Spectrometry, Fluorescence; D015027:Zebrafish / Q000033:anatomy & histology* / Q000196:embryology / Q000235:genetics
chemical_list: D015335:Molecular Probes; C485192:green fluorescent protein, Aequorea victoria; D049452:Green Fluorescent Proteins
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