title: Variation in the degree of pectin methylesterification during the development of Baccharis dracunculifolia kidney-shaped gall.
abstract: Insect galls may be study models to test the distribution of pectins and arabinogalactan-proteins (AGPs) and their related functions during plant cell cycles. These molecules are herein histochemically and immunocitochemically investigated in the kidney-shaped gall induced by Baccharopelma dracunculifoliae (Psyllidae) on leaves of Baccharis dracunculifolia DC. (Asteraceae) on developmental basis. The homogalacturonans (HGAs) (labeled by JIM5) and the arabinans (labeled by LM6) were detected either in non-galled leaves or in young galls, and indicated stiffening of epidermal cell walls, which is an important step for cell redifferentiation. The labeling of HGAs by JIM7 changed from young to senescent stage, with an increase in the rigidity of cell walls, which is important for the acquaintance of the final gall shape and for the mechanical opening of the gall. The variation on the degree of HGAs during gall development indicated differential PMEs activity during gall development. The epitopes recognized by LM2 (AGP glycan) and LM5 (1-4-β-D-galactans) had poor alterations from non-galled leaves towards gall maturation and senescence. Moreover, the dynamics of pectin and AGPs on two comparable mature kidney-shaped galls on B. dracunculifolia and on B. reticularia revealed specific peculiarities. Our results indicate that similar gall morphotypes in cogeneric host species may present distinct cell responses in the subcelular level, and also corroborate the functions proposed in literature for HGAs.
authors.affiliation: Universidade Federal de Uberlândia (UFU), Instituto de Biologia (INBIO), Campus Umuarama, Uberlândia, Minas Gerais, Brasil.
authors.forename: Denis Coelho
authors.initials: DC
authors.lastname: de Oliveira
authors.affiliation: Universidade Federal de Minas Gerais (UFMG), Instituto de Ciências Biológicas, Departamento de Botânica, Belo Horizonte, Minas Gerais, Brasil.
authors.forename: Thiago Alves
authors.initials: TA
authors.lastname: Magalhães
authors.affiliation: Universidade Federal de Minas Gerais (UFMG), Instituto de Ciências Biológicas, Departamento de Botânica, Belo Horizonte, Minas Gerais, Brasil.
authors.forename: Bruno Garcia
authors.initials: BG
authors.lastname: Ferreira
authors.affiliation: Universidade Federal de Minas Gerais (UFMG), Instituto de Ciências Biológicas, Departamento de Botânica, Belo Horizonte, Minas Gerais, Brasil.
authors.forename: Cristiane Trindade
authors.initials: CT
authors.lastname: Teixeira
authors.affiliation: Universidade Federal de Minas Gerais (UFMG), Instituto de Ciências Biológicas, Departamento de Botânica, Belo Horizonte, Minas Gerais, Brasil.
authors.forename: Anete Teixeira
authors.initials: AT
authors.lastname: Formiga
authors.affiliation: Universidade Federal de Minas Gerais (UFMG), Instituto de Ciências Biológicas, Departamento de Biologia Geral, Belo Horizonte, Minas Gerais, Brasil.
authors.forename: Geraldo Wilson
authors.initials: GW
authors.lastname: Fernandes
authors.affiliation: Universidade Federal de Minas Gerais (UFMG), Instituto de Ciências Biológicas, Departamento de Botânica, Belo Horizonte, Minas Gerais, Brasil.
authors.forename: Rosy Mary Dos Santos
authors.initials: RM
authors.lastname: Isaias
mesh_terms: D031175:Baccharis / Q000166:cytology / Q000378:metabolism*; D000939:Epitopes / Q000276:immunology; D004951:Esterification; D010368:Pectins / Q000276:immunology / Q000378:metabolism*; D018515:Plant Leaves / Q000166:cytology / Q000378:metabolism; D010941:Plant Tumors
chemical_list: D000939:Epitopes; D010368:Pectins
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