title: Site-specific analysis of inflammatory markers in discoid lupus erythematosus skin.
abstract: Prior studies identified T cells, B cells, and macrophages in the inflammatory infiltrate and up-regulation of their protein products in discoid lupus erythematosus (DLE) skin; however, they lacked rigorous analyses to define their specific locations in skin. Thus, we compared expressions of selected T cell, B cell, and macrophage markers in five areas of DLE, psoriasis, and normal skin. Immunostainings for CD3, CD4, CD8, CD20, CD68, CXCR3, CXCL10, and TIA-1 were performed in biopsies of 23 DLE lesional skin, 11 psoriasis lesional skin, and 5 normal skin. Three independent observers used a graded scale to rate each marker's presence in the epidermis, dermatoepidermal junction (DEJ), perivascular area, periadnexal area, and deep dermis. DLE lesional skin contained an increased abundance of CD3(+), CD8(+), and CD68(+) cells at the DEJ, and CD20(+) and CD68(+) cells in the periadnexal area versus psoriasis and normal skin. CXCR3, CXCL10, and TIA-1 were elevated in periadnexal sites of DLE lesional skin versus psoriasis lesional skin. The aggregation of T cells, B cells, macrophages, and their protein products (CXCR3, CXCL10, and TIA-1) in the DEJ and periadnexal area of DLE lesional skin may contribute to the pathology of DLE through a coordinated, sophisticated process.
authors.affiliation: Department of Dermatology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390-9069, USA.
authors.forename: Ryan B
authors.initials: RB
authors.lastname: Thorpe
authors.affiliation: Department of Pathology, University of Texas Southwestern Medical Center, Dallas, TX 75390-9072, USA.
authors.forename: Anna
authors.initials: A
authors.lastname: Gray
authors.affiliation: Department of Pathology, University of Texas Southwestern Medical Center, Dallas, TX 75390-9072, USA.
authors.forename: Kirthi R
authors.initials: KR
authors.lastname: Kumar
authors.affiliation: Department of Dermatology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390-9069, USA ; Cockerell Dermatopathology, Dallas, TX, USA.
authors.forename: Joseph S
authors.initials: JS
authors.lastname: Susa
authors.affiliation: Department of Dermatology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390-9069, USA.
authors.forename: Benjamin F
authors.initials: BF
authors.lastname: Chong
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D001706:Biopsy; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D018836:Inflammation Mediators / Q000378:metabolism*; D008179:Lupus Erythematosus, Discoid / Q000188:drug therapy / Q000276:immunology / Q000378:metabolism*; D016131:Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism; D008264:Macrophages / Q000276:immunology / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D012867:Skin / Q000378:metabolism* / Q000473:pathology*
chemical_list: D015415:Biomarkers; D018836:Inflammation Mediators
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