title: Manilkara zapota (Linn.) Seeds: A Potential Source of Natural Gum.
abstract: Mucilage isolated from seeds of Manilkara zapota (Linn.) P. Royen syn. is a plant growing naturally in the forests of India. This mucilage is yet to be commercially exploited, and characterized as polymer. Various physicochemical methods like particle size analysis, scanning electron microscopy, thermal analysis, gel permeation chromatography, X-ray diffraction spectrometry, zeta potential, Fourier transform infrared spectroscopy, and nuclear magnetic resonance spectroscopy have been employed to characterize this gum in the present study. Particle size analyses suggest that mucilage has particle size in nanometer. Scanning electron microscopy analysis suggests that the mucilage has irregular particle size. The glass transition temperature of the gum was observed to be 138°C and 136°C by differential scanning calorimetry and differential thermal analysis, respectively. The thermogravimetric analysis suggested that mucilage had good thermal stability. The average molecular weight of mucilage was determined to be 379180, by gel permeation chromatography, while the viscosity of mucilage was observed to be 219.1 cP. The X-ray diffraction spectrometry pattern of the mucilage indicates a completely amorphous structure. Elemental analysis of the gum revealed the contents of carbon, hydrogen, nitrogen, and sulfur to be 80.9 (%), 10.1 (%), 1.58 (%), and 512 (mg/kg), respectively. Mucilage had specific content of calcium, magnesium, potassium, lower concentrations of aluminum, cadmium, cobalt, lead, and nickel. The major functional groups identified from FT-IR spectrum include 3441 cm(-1) (-OH), 1660 cm(-1) (Alkenyl C-H & C=C Stretch), 1632 cm(-1) (-COO-), 1414 cm(-1) (-COO-), and 1219 cm(-1) (-CH3CO). Analysis of mucilage by paper chromatography and 1D NMR, indicated the presence of rhamnose, xylose, arabinose, mannose, and fructose.
authors.affiliation: H.S.B.P.V.T. Group of Institutions, College of Pharmacy, Kashti, Ahmednagar, Maharashtra 414701, India.
authors.forename: Sudarshan
authors.initials: S
authors.lastname: Singh
authors.affiliation: Bhagwan College of Pharmacy, Aurangabad, Maharashtra 431001, India.
authors.forename: Sunil B
authors.initials: SB
authors.lastname: Bothara
references.citation: Pharm Dev Technol. 2001;6(1):91-8
references.pmid: 11247279
references.citation: Eur J Pharm Biopharm. 2009 Jun;72(2):453-62
references.pmid: 18511246
references.citation: Phytochemistry. 1992 Oct;31(10):3307-30
references.pmid: 1368855
references.citation: Int J Biol Macromol. 2006 Feb 28;38(1):31-9
references.pmid: 16469374
references.citation: Curr Drug Deliv. 2004 Jul;1(3):195-201
references.pmid: 16305383
references.citation: J Pharm Sci. 2000 Jul;89(7):850-66
references.pmid: 10861586
references.citation: Fitoterapia. 2010 Sep;81(6):462-71
references.pmid: 20156530
references.citation: Int J Biol Macromol. 1992 Jun;14(3):166-9
references.pmid: 1390449
references.citation: Indian J Pharm Sci. 2008 Jul-Aug;70(4):415-22
references.pmid: 20046764
references.citation: Int J Pharm. 2002 Mar 2;234(1-2):1-17
references.pmid: 11839433