title: Cloning and expression of a cytosolic HSP90 gene in Chlorella vulgaris.
abstract: Heat shock protein 90 (HSP90), a highly conserved molecular chaperone, plays essential roles in folding, keeping structural integrity, and regulating the subset of cytosolic proteins. We cloned the cDNA of Chlorella vulgaris HSP90 (named CvHSP90) by combining homology cloning with rapid amplification of cDNA ends (RACE). Sequence analysis indicated that CvHSP90 is a cytosolic member of the HSP90 family. Quantitative RT-PCR was applied to determine the expression level of messenger RNA (mRNA) in CvHSP90 under different stress conditions. C. vulgaris was kept in different temperatures (5-45 °C) for 1 h. The mRNA expression level of CvHSP90 increased with temperature from 5 to 10 °C, went further from 35 to 40 °C, and reached the maximum at 40 °C. On the other hand, for C. vulgaris kept at 35 °C for different durations, the mRNA expression level of CvHSP90 increased gradually and reached the peak at 7 h and then declined progressively. In addition, the expression level of CvHSP90 at 40 or 45 in salinity (‰) was almost fourfold of that at 25 in salinity (‰) for 2 h. Therefore, CvHSP90 may be a potential biomarker to monitor environment changes.
authors.affiliation: Key Lab of Marine Biology in Jiang Su, College of Resources and Environmental Science, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Zhengyi
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Pu
authors.affiliation: Key Lab of Marine Biology in Jiang Su, College of Resources and Environmental Science, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Zhaopu
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Shandong Oriental Ocean Sci-Tech Co., Ltd., Yantai 264003, China.
authors.forename: Zhiling
authors.initials: Z
authors.lastname: Li
authors.affiliation: Shandong Oriental Ocean Sci-Tech Co., Ltd., Yantai 264003, China.
authors.forename: Yushan
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Key Lab of Marine Biology in Jiang Su, College of Resources and Environmental Science, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Song
authors.initials: S
authors.lastname: Qin
mesh_terms: D000595:Amino Acid Sequence; D015415:Biomarkers; D048408:Chlorella vulgaris / Q000235:genetics*; D003001:Cloning, Molecular; D004784:Environmental Monitoring; D005786:Gene Expression Regulation; D018841:HSP90 Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics*; D010802:Phylogeny; D054712:Salinity; D013312:Stress, Physiological
chemical_list: D015415:Biomarkers; D018841:HSP90 Heat-Shock Proteins
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