title: ZIP1 and zinc inhibits fluoride-induced apoptosis in MC3T3-E1 cells.
abstract: Excess fluoride intake could induce apoptosis in the cells. As an essential micronutrient and cytoprotectant, zinc is involved in many types of apoptosis. Here, we studied the effects of zinc and ZIP1 on fluoride-induced apoptosis in mouse MC3T3-E1 cells and examined the underlying molecular mechanisms. Our study found that fluoride not only inhibited cell proliferation and increased the intracellular reactive oxygen species (ROS) but also induced cell apoptosis. Whereas pretreatment with zinc significantly attenuated fluoride-induced ROS production and partly protected cells against fluoride-induced apoptosis through MAPK/ERK signaling pathway. Our study also found that fluoride upregulated the expression of ZIP1 in a time-dependent manner. Moreover, overexpression of ZIP1 also inhibited fluoride-induced apoptosis by activation of PI3K/Akt pathway. This cytoprotective effect of zinc and ZIP1 may be new factors that affect the physiological activity of fluoride and need study further.
authors.affiliation: Department of Orthopaedics, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong, 250021, People's Republic of China.
authors.forename: Shihong
authors.initials: S
authors.lastname: Xu
authors.forename: Yongliang
authors.initials: Y
authors.lastname: Yang
authors.forename: Shumei
authors.initials: S
authors.lastname: Han
authors.forename: Zonghui
authors.initials: Z
authors.lastname: Wu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D027682:Cation Transport Proteins / Q000378:metabolism*; D002460:Cell Line; D005434:Flow Cytometry; D005459:Fluorides / Q000494:pharmacology*; D051379:Mice; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction; D015032:Zinc / Q000494:pharmacology*; D017967:Zinc Compounds / Q000494:pharmacology*
chemical_list: D027682:Cation Transport Proteins; D017382:Reactive Oxygen Species; C485744:Slc39a1 protein, mouse; D017967:Zinc Compounds; D015032:Zinc; C038904:zinc fluoride; D005459:Fluorides