title: Influenza in seasons 2009-2013 in the Faculty Hospital Hradec Kralove, East Bohemia.
abstract: OBJECTIVE
  The evaluation of four post-pandemic influenza seasons 2009-2013 in the Faculty hospital Hradec Králové and comparison of used rapid antigen tests (RATs) with real time RT-PCR.
  
  
  METHODS
  Between November 2009 and June 2013 were examined 3845 samples from patients with respiratory tract infections by RATs (Influenza A/B 2 Panel Test (GECKO® Pharma, Germany), Rapid VIDITEST Influenza A+B Card (VIDIA®, Czech Republic) and BinaxNOW Influenza A&B Test (ALERE®, USA) or real time RT-PCR (RTR InfA/H1N1 Detection Set (Roche®), RealStar® Influenza S and T RT-PCR Kit 3.0 (Altona ®, Germany).
  
  
  RESULTS
  A totally 1059 samples were examined simultaneously by RAT and real time RT-PCR. The overall sensitivity and specificity of RATs compared with real time RT-PCR were 32,2 % and 98,1 % for influenza A and 17,6 % and 99,4 % for influenza B. Higher sensitivity of RATs were in children (66,6 %) compared with adults (14,3 - 40,0 %). In the first three post-pandemic seasons were continuously decrease of positive samples from 23,5 % in season 2009-2010 to 3,3% in season 2011-2012, but in season 2012-2013 were rapidly in-crease of positive results, to 31,5%, with high share of influenza -A/H1N1/2009 (79,6%).
  
  
  CONCLUSIONS
  Our results shown insufficient sensitivity of all used RATs and necessity of having other confirmatory test, like RT-PCR. It was also shown unexplained increase of case and influenza severity in season 2012-2013.
authors.forename: M
authors.initials: M
authors.lastname: Fajfr
authors.forename: V
authors.initials: V
authors.lastname: Stěpánová
authors.forename: L
authors.initials: L
authors.lastname: Plíšková
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000956:Antigens, Viral / Q000032:analysis; D002648:Child; D018153:Czech Republic / Q000453:epidemiology; D005260:Female; D006801:Humans; D007223:Infant; D053118:Influenza A Virus, H1N1 Subtype; D007251:Influenza, Human / Q000175:diagnosis* / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D060888:Real-Time Polymerase Chain Reaction; D012189:Retrospective Studies; D013997:Time Factors; D000090324:Tumor Protein, Translationally-Controlled 1
chemical_list: D000956:Antigens, Viral; C000715649:Tpt1 protein, rat; D000090324:Tumor Protein, Translationally-Controlled 1