title: N,N,N-Trimethyl chitosan nanoparticles for controlled intranasal delivery of HBV surface antigen.
abstract: Hepatitis B virus surface antigen (HBsAg) loaded N,N,N-trimethyl chitosan nanoparticles (N-TMC NPs) were formulated and studied for controlled intranasal delivery. The size and surface properties of the NPs can be tuned by modifying the concentration of N-TMC and found to be 66±13, 76±9 nm for 0.25 and 0.5 wt.% respectively. Loading of 380 and 760 μl of HBsAg yielded 143±33, 259±47 nm sized spherical N-TMC NPs with highest loading efficiency and capacity of 90-93%, and 96-97% respectively. In vitro drug release analysis ensured 93% cumulative release of HBsAg antigen over prolonged period (43 days). In vivo immunological study was performed using 6-8 weeks old female BALB mice and reveals adjuvants efficiency of NPs for antigen is highly stable and better than standard. Obtained results show that N-TMC NPs can be extensively used in controlled intra nasal delivery to treat various diseases including hepatitis B and allergic rhinitis.
authors.affiliation: University of Science and Technology (UST), 113 Gwahangno, Yuseong-gu, Daejon 305333, Republic of Korea; Biomaterials Center, Korea Institute of Science and Technology (KIST), Hawolgok, Seoul 136791, Republic of Korea.
authors.forename: Ramesh
authors.initials: R
authors.lastname: Subbiah
authors.affiliation: Department of Pharmaceutics, Nandha College of Pharmacy, Erode, Tamil Nadu 638052, India.
authors.forename: Prakash
authors.initials: P
authors.lastname: Ramalingam
authors.affiliation: Biomaterials Center, Korea Institute of Science and Technology (KIST), Hawolgok, Seoul 136791, Republic of Korea.
authors.forename: Subramaniyan
authors.initials: S
authors.lastname: Ramasundaram
authors.affiliation: Biomaterials Center, Korea Institute of Science and Technology (KIST), Hawolgok, Seoul 136791, Republic of Korea.
authors.forename: Do Yang
authors.initials: DY
authors.lastname: Kim
authors.affiliation: Biomaterials Center, Korea Institute of Science and Technology (KIST), Hawolgok, Seoul 136791, Republic of Korea.
authors.forename: Kwideok
authors.initials: K
authors.lastname: Park
authors.affiliation: Department of Pharmaceutics, Nandha College of Pharmacy, Erode, Tamil Nadu 638052, India.
authors.forename: Mohan K
authors.initials: MK
authors.lastname: Ramasamy
authors.affiliation: School of Mechanical & Advanced Materials Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan 698805, Republic of Korea.
authors.forename: Kyoung Jin
authors.initials: KJ
authors.lastname: Choi
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D048271:Chitosan / Q000737:chemistry / Q000494:pharmacology; D003692:Delayed-Action Preparations / Q000737:chemistry / Q000494:pharmacology; D004337:Drug Carriers / Q000737:chemistry / Q000494:pharmacology; D005260:Female; D006514:Hepatitis B Surface Antigens / Q000737:chemistry / Q000494:pharmacology; D017325:Hepatitis B Vaccines / Q000737:chemistry / Q000494:pharmacology; D006515:Hepatitis B virus / Q000276:immunology; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D053758:Nanoparticles / Q000737:chemistry*
chemical_list: D003692:Delayed-Action Preparations; D004337:Drug Carriers; D006514:Hepatitis B Surface Antigens; D017325:Hepatitis B Vaccines; C118071:N-trimethyl chitosan chloride; D048271:Chitosan