title: Expression of peanut Iron Regulated Transporter 1 in tobacco and rice plants confers improved iron nutrition.
abstract: Iron (Fe) limitation is a widespread agricultural problem in calcareous soils and severely limits crop production. Iron Regulated Transporter 1 (IRT1) is a key component for Fe uptake from the soil in dicot plants. In this study, the peanut (Arachis hypogaea L.) AhIRT1 was introduced into tobacco and rice plants using an Fe-deficiency-inducible artificial promoter. Induced expression of AhIRT1 in tobacco plants resulted in accumulation of Fe in young leaves under Fe deficient conditions. Even under Fe-excess conditions, the Fe concentration was also markedly enhanced, suggesting that the Fe status did not affect the uptake and translocation of Fe by AhIRT1 in the transgenic plants. Most importantly, the transgenic tobacco plants showed improved tolerance to Fe limitation in culture in two types of calcareous soils. Additionally, the induced expression of AhIRT1 in rice plants also resulted in high tolerance to low Fe availability in calcareous soils.
authors.affiliation: Key Laboratory of Plant-Soil Interactions, MOE, Centre for Resource, Environment and Food Security, China Agricultural University, Beijing 100193, China.
authors.forename: Hongchun
authors.initials: H
authors.lastname: Xiong
authors.affiliation: Key Laboratory of Plant-Soil Interactions, MOE, Centre for Resource, Environment and Food Security, China Agricultural University, Beijing 100193, China.
authors.forename: Xiaotong
authors.initials: X
authors.lastname: Guo
authors.affiliation: Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan; Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University, 1-308 Suematsu, Nonoichi, Ishikawa 921-8836, Japan.
authors.forename: Takanori
authors.initials: T
authors.lastname: Kobayashi
authors.affiliation: Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Kakei
authors.affiliation: Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Hiromi
authors.initials: H
authors.lastname: Nakanishi
authors.affiliation: Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Nozoye
authors.affiliation: Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Lixia
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Plant-Soil Interactions, MOE, Centre for Resource, Environment and Food Security, China Agricultural University, Beijing 100193, China.
authors.forename: Hongyun
authors.initials: H
authors.lastname: Shen
authors.affiliation: Key Laboratory of Plant-Soil Interactions, MOE, Centre for Resource, Environment and Food Security, China Agricultural University, Beijing 100193, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Qiu
authors.affiliation: Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan; Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University, 1-308 Suematsu, Nonoichi, Ishikawa 921-8836, Japan.
authors.forename: Naoko K
authors.initials: NK
authors.lastname: Nishizawa
authors.affiliation: Key Laboratory of Plant-Soil Interactions, MOE, Centre for Resource, Environment and Food Security, China Agricultural University, Beijing 100193, China. Electronic address: zuoym@cau.edu.cn.
authors.forename: Yuanmei
authors.initials: Y
authors.lastname: Zuo
mesh_terms: D010367:Arachis / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D007501:Iron / Q000378:metabolism*; D012275:Oryza / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism*; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D007501:Iron
keywords: Calcareous soil; IRT1; Iron deficiency; Peanut; Transgenic plant