title: Structural functional and folding scenario of an anti platelet and thrombolytic enzyme crinumin.
abstract: A folding pattern, conformational stability and therapeutic role of a protein helps in developing a suitable drug. Crinumin, a thrombolytic and anti platelet agent, has been studied for its functional and conformational properties by equilibrium unfolding methods. The crinumin belongs to α+β class of protein and exhibits a non native structure and two molten globule states at different conditions. Two domains in the molecular structure of the protein with altered stability are present that unfold sequentially. The enzyme maintains activity as well as structural integrity even in adverse conditions. These observations provide an understanding of protein folding as well as facilitate the development of a potential drug.
authors.affiliation: Department of Pharmaceutics, Indian Institute of Technology, Banaras Hindu University, Varanasi, UP 221 005, India. Electronic address: kawaneesh@gmail.com.
authors.forename: Kunwar Awaneesh
authors.initials: KA
authors.lastname: Singh
authors.affiliation: Department of Pharmaceutics, Indian Institute of Technology, Banaras Hindu University, Varanasi, UP 221 005, India.
authors.forename: Sanjay
authors.initials: S
authors.lastname: Singh
authors.affiliation: Molecular Biology Unit, Institute of Medical Sciences, Banaras Hindu University, Varanasi, UP 221 005, India. Electronic address: jvm@bhu.ac.in.
authors.forename: Medicherla V
authors.initials: MV
authors.lastname: Jagannadham
mesh_terms: D002942:Circular Dichroism; D005343:Fibrinolytic Agents / Q000737:chemistry* / Q000378:metabolism*; D019791:Guanidine / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D010940:Plant Proteins / Q000737:chemistry* / Q000378:metabolism*; D010975:Platelet Aggregation Inhibitors / Q000737:chemistry* / Q000378:metabolism*; D011489:Protein Denaturation / Q000187:drug effects; D017510:Protein Folding / Q000187:drug effects; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013329:Structure-Activity Relationship; D013696:Temperature; D014508:Urea / Q000494:pharmacology
chemical_list: D005343:Fibrinolytic Agents; D010940:Plant Proteins; D010975:Platelet Aggregation Inhibitors; C569001:crinumin, Crinum asiaticum; D014508:Urea; D019791:Guanidine
keywords: Anti-platelet; Crinumin; Protein folding; Thrombolytic