title: Rapid screening of multiple antibiotic residues in milk using disposable amperometric magnetosensors.
abstract: Disposable amperometric magnetosensors, involving a mixture of modified-magnetic beads (MBs), for the multiplex screening of cephalosporins (CPHs), sulfonamides (SAs) and tetracyclines (TCs) antibiotic residues in milk are reported for the first time in this work. The multiplexed detection relies on the use of a mixture of target specific modified magnetic beads (MBs) and application of direct competitive assays using horseradish peroxidase (HRP)-labeled tracers. The amperometric responses measured at -0.20 V vs. the Ag pseudo-reference electrode of screen-printed carbon electrodes (SPCE) upon the addition of H2O2 in the presence of hydroquinone (HQ) as redox mediator, were used to monitor the extent of the different affinity reactions. The developed methodology, involving a simple and short pretreatment, allowed discrimination between no contaminated UHT and raw milk samples and samples containing antibiotic residues at the maximum residue limits (MRLs). The usefulness of the multiplexed magnetosensor was demonstrated by analyzing spiked milk samples in only 5 min. The results demonstrated that a clear discrimination of milk samples contaminated with antibiotics at their MRL level or their mixtures, allowing the identification of milk not complying with current legislation. These features make the developed methodology a promising alternative in the development of user-friendly devices for on-site analysis to ensure quality control for dairy products.
authors.affiliation: Departamento de Química Analítica, Facultad de Ciencias Químicas, Universidad Complutense de Madrid, Avenida Complutense s/n, 28040 Madrid, Spain. Electronic address: felipecf@quim.ucm.es.
authors.forename: F
authors.initials: F
authors.lastname: Conzuelo
authors.affiliation: Departamento de Química Analítica, Facultad de Ciencias Químicas, Universidad Complutense de Madrid, Avenida Complutense s/n, 28040 Madrid, Spain. Electronic address: victor_lega90@hotmail.com.
authors.forename: V
authors.initials: V
authors.lastname: Ruiz-Valdepeñas Montiel
authors.affiliation: Departamento de Química Analítica, Facultad de Ciencias Químicas, Universidad Complutense de Madrid, Avenida Complutense s/n, 28040 Madrid, Spain. Electronic address: susanacr@quim.ucm.es.
authors.forename: S
authors.initials: S
authors.lastname: Campuzano
authors.affiliation: Departamento de Química Analítica, Facultad de Ciencias Químicas, Universidad Complutense de Madrid, Avenida Complutense s/n, 28040 Madrid, Spain. Electronic address: mariagam@quim.ucm.es.
authors.forename: M
authors.initials: M
authors.lastname: Gamella
authors.affiliation: Departamento de Química Analítica, Facultad de Ciencias Químicas, Universidad Complutense de Madrid, Avenida Complutense s/n, 28040 Madrid, Spain. Electronic address: rebeca.magnolia@gmail.com.
authors.forename: R M
authors.initials: RM
authors.lastname: Torrente-Rodríguez
authors.affiliation: Departamento de Química Analítica, Facultad de Ciencias Químicas, Universidad Complutense de Madrid, Avenida Complutense s/n, 28040 Madrid, Spain. Electronic address: reviejo@quim.ucm.es.
authors.forename: A J
authors.initials: AJ
authors.lastname: Reviejo
authors.affiliation: Departamento de Química Analítica, Facultad de Ciencias Químicas, Universidad Complutense de Madrid, Avenida Complutense s/n, 28040 Madrid, Spain. Electronic address: pingarro@quim.ucm.es.
authors.forename: J M
authors.initials: JM
authors.lastname: Pingarrón
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation*; D004209:Disposable Equipment; D004350:Drug Residues / Q000032:analysis* / Q000737:chemistry; D004563:Electrochemistry; D004566:Electrodes; D006735:Horseradish Peroxidase / Q000378:metabolism; D059346:Magnets / Q000737:chemistry*; D008863:Microspheres; D008892:Milk / Q000737:chemistry*; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D006735:Horseradish Peroxidase
keywords: Cephalosporins; Magnetic beads; Milk; Screen-printed electrodes; Sulfonamides; Tetracyclines