title: Ultrasensitive electrochemical immunoassay for avian influenza subtype H5 using nanocomposite.
abstract: We report a novel electrochemical immunosensor that can sensitively detect avian influenza virus H5 subtype (AIV H5) captured by graphene oxide-H5-polychonal antibodies-bovine serum albumin (GO-PAb-BSA) nanocomposite. The graphene oxide (GO) carried H5-polychonal antibody (PAb) were used as signal amplification materials. Upon signal amplification, the immunosensor showed a 256-fold increase in detection sensitivity compared to the immunosensor without GO-PAb-BSA. We designed a PAb labeling GO strategy and signal amplification procedure that allow ultrasensitive and selective detection of AIV H5. The established method responded to 2(-15) HA unit/50 µL H5, with a linear calibration range from 2(-15) to 2(-8) HA unit/50 µL. In summary, we demonstrated that the immunosenser has a high specificity and sensitivity for AIV H5, and the established assay could be potentially applied in the rapid detection of other pathogenic microorganisms.
authors.affiliation: Guangxi Key Laboratory of Animal Vaccines and Diagnostics, Guangxi Veterinary Research Institute, Nanning, Guangxi Province, China.
authors.forename: Zhixun
authors.initials: Z
authors.lastname: Xie
authors.affiliation: Guangxi Key Laboratory of Animal Vaccines and Diagnostics, Guangxi Veterinary Research Institute, Nanning, Guangxi Province, China.
authors.forename: Jiaoling
authors.initials: J
authors.lastname: Huang
authors.affiliation: Guangxi Key Laboratory of Animal Vaccines and Diagnostics, Guangxi Veterinary Research Institute, Nanning, Guangxi Province, China.
authors.forename: Sisi
authors.initials: S
authors.lastname: Luo
authors.affiliation: Guangxi Key Laboratory of Animal Vaccines and Diagnostics, Guangxi Veterinary Research Institute, Nanning, Guangxi Province, China.
authors.forename: Zhiqin
authors.initials: Z
authors.lastname: Xie
authors.affiliation: Guangxi Key Laboratory of Animal Vaccines and Diagnostics, Guangxi Veterinary Research Institute, Nanning, Guangxi Province, China.
authors.forename: Liji
authors.initials: L
authors.lastname: Xie
authors.affiliation: Guangxi Key Laboratory of Animal Vaccines and Diagnostics, Guangxi Veterinary Research Institute, Nanning, Guangxi Province, China.
authors.forename: Jiabo
authors.initials: J
authors.lastname: Liu
authors.affiliation: Guangxi Key Laboratory of Animal Vaccines and Diagnostics, Guangxi Veterinary Research Institute, Nanning, Guangxi Province, China.
authors.forename: Yaoshan
authors.initials: Y
authors.lastname: Pang
authors.affiliation: Guangxi Key Laboratory of Animal Vaccines and Diagnostics, Guangxi Veterinary Research Institute, Nanning, Guangxi Province, China.
authors.forename: Xianwen
authors.initials: X
authors.lastname: Deng
authors.affiliation: Guangxi Key Laboratory of Animal Vaccines and Diagnostics, Guangxi Veterinary Research Institute, Nanning, Guangxi Province, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Fan
mesh_terms: D000818:Animals; D000941:Antigens / Q000737:chemistry; D015374:Biosensing Techniques; D001717:Birds; D002138:Calibration; D002417:Cattle; D002642:Chick Embryo / Q000821:virology; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D006108:Graphite / Q000737:chemistry; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D007118:Immunoassay / Q000379:methods; D053124:Influenza A Virus, H5N1 Subtype / Q000302:isolation & purification*; D005585:Influenza in Birds / Q000821:virology*; D053761:Nanocomposites; D010087:Oxides / Q000737:chemistry; D012709:Serum Albumin / Q000737:chemistry
chemical_list: D000941:Antigens; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D010087:Oxides; D012709:Serum Albumin; D006108:Graphite
references.citation: Lancet. 1998 Feb 14;351(9101):467-71
references.pmid: 9482437
references.citation: Biotechnol Adv. 2011 Mar-Apr;29(2):169-88
references.pmid: 21034805
references.citation: Talanta. 2007 Dec 15;74(3):308-17
references.pmid: 18371644
references.citation: Mol Cell Probes. 2006 Jun-Aug;20(3-4):245-9
references.pmid: 16542820
references.citation: Chem Rev. 2012 Nov 14;112(11):6027-53
references.pmid: 22889102
references.citation: Sensors (Basel). 2012;12(9):12506-18
references.pmid: 23112728
references.citation: Am J Vet Res. 1968 Nov;29(11):2163-71
references.pmid: 5693465
references.citation: Anal Chem. 2011 Feb 1;83(3):746-52
references.pmid: 21210663
references.citation: J Clin Microbiol. 1999 Apr;37(4):937-43
references.pmid: 10074505
references.citation: J Clin Microbiol. 2000 Nov;38(11):4096-101
references.pmid: 11060074
references.citation: Mol Cell Probes. 2008 Oct-Dec;22(5-6):287-93
references.pmid: 18672052
references.citation: J Virol Methods. 2004 Aug;119(2):151-8
references.pmid: 15158597
references.citation: ACS Appl Mater Interfaces. 2011 Apr;3(4):1366-73
references.pmid: 21446771
references.citation: PLoS One. 2009;4(3):e4682
references.pmid: 19270752
references.citation: Anal Bioanal Chem. 2005 Jan;381(1):156-64
references.pmid: 15592819
references.citation: J Virol Methods. 2008 Feb;147(2):219-25
references.pmid: 17950911
references.citation: Anal Chem. 2012 Feb 21;84(4):1871-8
references.pmid: 22409529
references.citation: Talanta. 2009 Jul 15;79(2):159-64
references.pmid: 19559858
references.citation: J Virol Methods. 2008 Dec;154(1-2):213-5
references.pmid: 18793671
references.citation: Analyst. 2011 Dec 21;136(24):5204-10
references.pmid: 22005508