title: Intraurethral injection of autologous minced skeletal muscle: a simple surgical treatment for stress urinary incontinence.
abstract: OBJECTIVE
  Intraurethral injection of in vitro expanded autologous skeletal muscle derived cells is a new regenerative therapy for stress urinary incontinence. We examined the efficacy and safety of a simpler alternative strategy using freshly harvested, minced autologous skeletal muscle tissue with its inherent content of regenerative cells.
  
  
  METHODS
  A total of 20 and 15 women with uncomplicated and complicated stress urinary incontinence, respectively, received intraurethral injections of minced autologous skeletal muscle tissue and were followed for 1 year. Efficacy was assessed by the number of leakages in a 3-day diary and by ICIQ-SF scores. We calculated the rates of cure, defined as zero leaks in 3 days plus an ICIQ-SF score of 5 or less, and improvement, defined as simultaneous decreases in each outcome measure.
  
  
  RESULTS
  Significant reductions were observed in each group in the mean number of leakages (p <0.01) and in ICIQ-SF scores (p <0.001). In the uncomplicated group cure and improvement were observed in 25% and 63% of patients, and in the complicated group they were noted in 7% and 57%, respectively. No voiding dysfunction developed and only minor adverse events were noted.
  
  
  CONCLUSIONS
  Intraurethral injection of minced autologous muscle tissue is a simple surgical procedure that appears safe and moderately effective in women with uncomplicated stress urinary incontinence. It compares well to a more complicated regenerative strategy using in vitro expanded muscle derived cells.
authors.affiliation: Department of Obstetrics and Gynecology, Copenhagen University Hospital Herlev, Herlev, Denmark. Electronic address: s.gras@dadlnet.dk.
authors.forename: Søren
authors.initials: S
authors.lastname: Gräs
authors.affiliation: Department of Obstetrics and Gynecology, Copenhagen University Hospital Herlev, Herlev, Denmark.
authors.forename: Niels
authors.initials: N
authors.lastname: Klarskov
authors.affiliation: Department of Obstetrics and Gynecology, Copenhagen University Hospital Herlev, Herlev, Denmark.
authors.forename: Gunnar
authors.initials: G
authors.lastname: Lose
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017690:Cell Transplantation; D005260:Female; D006801:Humans; D007267:Injections; D008875:Middle Aged; D018482:Muscle, Skeletal / Q000166:cytology / Q000637:transplantation*; D010865:Pilot Projects; D012038:Regeneration; D014521:Urethra / Q000502:physiology; D014550:Urinary Incontinence, Stress / Q000601:surgery*; D013520:Urologic Surgical Procedures / Q000379:methods
keywords: quadriceps muscle; regeneration; stem cells; stress; urethra; urinary incontinence