title: Novosphingobium kunmingense sp. nov., isolated from a phosphate mine.
abstract: A yellow-pigmented, Gram-stain-negative, strictly aerobic, rod-shaped, round-ended bacterium, designated strain 18-11HK(T), was isolated from a phosphate mine situated in the suburb of Kunming in Yunnan province in south-western China. The taxonomic status of this strain was evaluated by using a polyphasic approach. On the basis of 16S rRNA gene sequence similarity, strain 18-11HK(T) was shown to belong to the genus Novosphingobium, showing the highest levels of sequence similarity with respect to 'Novosphingobium ginsenosidimutans' FW-6 (97.2%), Novosphingobium subterraneum DSM 12447(T) (96.7%), Novosphingobium aromaticivorans DSM 12444(T) (96.7%) and Novosphingobium tardaugens DSM 16702(T) (96.3%). Strain 18-11HK(T) had a genomic DNA G+C content of 65.3 mol% and Q-10 as the predominant respiratory quinone. DNA-DNA hybridizations of strain 18-11HK(T) with N. subterraneum DSM 12447(T), N. aromaticivorans DSM 12444(T) and N. tardaugens DSM 16702(T) showed low relatedness values of 29.6, 33.5 and 32.3%, respectively. The predominant fatty acids of strain 18-11HK(T) were summed feature 8 (C18 : 1ω7c and/or C18: 1ω6c), summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and C16 : 0, and the major 2-hydroxy fatty acid was C14 : 0 2-OH. The polar lipid profile revealed the presence of sphingoglycolipid, phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine and some unidentified lipids. On the basis of its phenotypic properties and phylogenetic distinctiveness, strain 18-11HK(T) represents a novel species of the genus Novosphingobium, for which the name Novosphingobium kunmingense sp. nov. is proposed. The type strain is 18-11HK(T) ( = CGMCC 1.12274(T) = DSM 25975(T)).
authors.affiliation: Henan Engineering Research Center of Industrial Enzymes, Biology Institute of Henan Academy of Sciences, Zhengzhou 450008, PR China.
authors.forename: Fuhong
authors.initials: F
authors.lastname: Xie
authors.affiliation: Henan Engineering Research Center of Industrial Enzymes, Biology Institute of Henan Academy of Sciences, Zhengzhou 450008, PR China.
authors.forename: Shujing
authors.initials: S
authors.lastname: Quan
authors.affiliation: Henan Engineering Research Center of Industrial Enzymes, Biology Institute of Henan Academy of Sciences, Zhengzhou 450008, PR China.
authors.forename: Dehai
authors.initials: D
authors.lastname: Liu
authors.affiliation: Henan Engineering Research Center of Industrial Enzymes, Biology Institute of Henan Academy of Sciences, Zhengzhou 450008, PR China.
authors.forename: Weihong
authors.initials: W
authors.lastname: He
authors.affiliation: Henan Engineering Research Center of Industrial Enzymes, Biology Institute of Henan Academy of Sciences, Zhengzhou 450008, PR China.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Henan Engineering Research Center of Industrial Enzymes, Biology Institute of Henan Academy of Sciences, Zhengzhou 450008, PR China.
authors.forename: Huan
authors.initials: H
authors.lastname: Ma
authors.affiliation: Henan Engineering Research Center of Industrial Enzymes, Biology Institute of Henan Academy of Sciences, Zhengzhou 450008, PR China.
authors.forename: Guocan
authors.initials: G
authors.lastname: Chen
authors.affiliation: State Key Laboratories of Transducer Technology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Yapeng
authors.initials: Y
authors.lastname: Chao
authors.affiliation: State Key Laboratories of Transducer Technology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Shijun
authors.initials: S
authors.lastname: Qian
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D008906:Mining; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010710:Phosphates / Q000737:chemistry*; D010802:Phylogeny; D010858:Pigmentation; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D042301:Sphingomonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010710:Phosphates; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C025204:Ubiquinone Q2