title: Conversion of epiblast stem cells to embryonic stem cells using growth factors and small molecule inhibitors.
abstract: Stem cell in vitro culture is a useful model system to study mechanisms underlying transitions between defined cell states. Epiblast stem cells, in addition to being capable of somatic differentiation, can be converted to a more primitive embryonic stem cell-like state, by overexpression of specific transcription factors. Here, we describe a reliable method to accomplish-and potentially further study-the transgene-independent reversion from epiblast stem cells to ES cells using administration of specific growth factors and small molecule inhibitors.
authors.affiliation: Human Stem Cell Pluripotency Laboratory, Max Planck Institute for Molecular Biomedicine, Von-Esmarch-Straße 54, 48149, Münster, Germany.
authors.forename: Jyoti
authors.initials: J
authors.lastname: Rao
authors.forename: Boris
authors.initials: B
authors.lastname: Greber
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D065150:Cellular Reprogramming / Q000187:drug effects*; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D005855:Germ Layers / Q000166:cytology*; D006801:Humans; D053605:Leukemia Inhibitory Factor / Q000494:pharmacology*; D051379:Mice; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000187:drug effects
chemical_list: D004791:Enzyme Inhibitors; D053605:Leukemia Inhibitory Factor; D016222:Fibroblast Growth Factor 2