title: α-Chitin nanofibrils improve inflammatory and fibrosis responses in inflammatory bowel disease mice model.
abstract: We evaluated the anti-inflammatory and anti-fibrosis effects of α-chitin nanofibrils in a mouse model of dextran sulfate sodium (DSS)-induced acute ulcerative colitis (UC). α-Chitin nanofibrils decreased positive areas of nuclear factor-κB staining in the colon tissue (7.2±0.5%/fields in the α-chitin nanofibrils group vs. 10.7±0.9%/fields in the control group; p<0.05). α-Chitin nanofibrils also decreased serum monocyte chemotactic protein-1 concentration in DSS-induced acute UC (24.1±7.8 pg/ml in the α-chitin nanofibrils group vs. 53.5±3.1 pg/ml in the control group; p<0.05). Moreover, α-chitin nanofibrils suppressed the increased positive areas of Masson's trichrome staining in colon tissue (6.8±0.6%/fields in the α-chitin nanofibrils group vs. 10.1±0.7%/fields in the control group; p<0.05). On the other hand, α-chitin powder suspension did not show these effects in DSS-induced acute UC mice model. Our results indicated that α-chitin nanofibrils have the anti-inflammatory effect via suppressing NF-κB activation and the anti-fibrosis effects in DSS-induced acute UC mice model.
authors.affiliation: Faculty of Agriculture, Tottori University, Tottori 680-8553, Japan.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Azuma
authors.affiliation: Faculty of Agriculture, Tottori University, Tottori 680-8553, Japan.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Osaki
authors.affiliation: Graduated School of Engineering, Tottori University, Tottori 680-8550, Japan.
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Ifuku
authors.affiliation: Graduated School of Engineering, Tottori University, Tottori 680-8550, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Saimoto
authors.affiliation: Faculty of Agriculture, Tottori University, Tottori 680-8553, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Tsuka
authors.affiliation: Faculty of Agriculture, Tottori University, Tottori 680-8553, Japan.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Imagawa
authors.affiliation: Faculty of Agriculture, Tottori University, Tottori 680-8553, Japan.
authors.forename: Yoshiharu
authors.initials: Y
authors.lastname: Okamoto
authors.affiliation: Faculty of Agriculture, Tottori University, Tottori 680-8553, Japan.
authors.forename: Saburo
authors.initials: S
authors.lastname: Minami
mesh_terms: D000818:Animals; D002686:Chitin / Q000737:chemistry* / Q000494:pharmacology / Q000627:therapeutic use; D004195:Disease Models, Animal; D005260:Female; D005355:Fibrosis; D018836:Inflammation Mediators / Q000037:antagonists & inhibitors* / Q000378:metabolism; D015212:Inflammatory Bowel Diseases / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D057139:Nanofibers / Q000737:chemistry* / Q000627:therapeutic use; D011897:Random Allocation; D016896:Treatment Outcome
chemical_list: D018836:Inflammation Mediators; D002686:Chitin