title: Simulating NIRS and MRS measurements during cerebral hypoxia-ischaemia in piglets using a computational model.
abstract: We present a group analysis of the changes in cerebral haemodynamics, and the oxidation state of cytochrome-c-oxidase measured using broadband near-infrared spectroscopy (NIRS) and intracellular pH measured by phosphorous ((31)P) magnetic resonance spectroscopy (MRS) during and after cerebral hypoxia-ischaemia (HI) in 15 piglets. We use a previously published computational model of cerebral metabolism in the piglet [1] to integrate these measurements and simulate HI. We successfully simulate changes in cellular metabolism including shifts in intracellular pH observed in the piglet brain during HI. In this process, we optimise physiological parameters in the model identified through sensitivity analysis (such as the rate of glucose metabolism and intracellular lactate concentration), to fit simulated and measured data. The model fits the data reasonably and suggests a 20 % drop in glucose consumption, a ~65 % increase in lactate concentration and ~35 % drop in the cerebral metabolic rate of oxygen (CMRO₂) during HI.
authors.affiliation: CoMPLEX, University College London, London, UK. t.hapuarachchi@ucl.ac.uk.|Department of Medical Physics and Bioengineering, University College London, Room 3.18, Malet Place Engineering Building, Gower Street, London, WC1E 6BT, UK. t.hapuarachchi@ucl.ac.uk.
authors.forename: T
authors.initials: T
authors.lastname: Hapuarachchi
authors.affiliation: CoMPLEX, University College London, London, UK.
authors.forename: T
authors.initials: T
authors.lastname: Moroz
authors.affiliation: Medical Physics and Bioengineering, University College London Hospitals, London, UK.
authors.forename: A
authors.initials: A
authors.lastname: Bainbridge
authors.affiliation: Institute for Women's Health, University College London, London, UK.
authors.forename: S
authors.initials: S
authors.lastname: Faulkner
authors.affiliation: Medical Physics and Bioengineering, University College London Hospitals, London, UK.
authors.forename: D
authors.initials: D
authors.lastname: Price
authors.affiliation: Institute for Women's Health, University College London, London, UK.
authors.forename: K D
authors.initials: KD
authors.lastname: Broad
authors.affiliation: Institute of Neurology, University College London, London, UK.
authors.forename: D
authors.initials: D
authors.lastname: Thomas
authors.affiliation: Medical Physics and Bioengineering, University College London Hospitals, London, UK.
authors.forename: E
authors.initials: E
authors.lastname: Cady
authors.affiliation: Institute of Neurology, University College London, London, UK.
authors.forename: X
authors.initials: X
authors.lastname: Golay
authors.affiliation: Institute for Women's Health, University College London, London, UK.
authors.forename: Nicola
authors.initials: N
authors.lastname: Robertson
authors.affiliation: Department of Medical Physics and Bioengineering, University College London, Room 3.18, Malet Place Engineering Building, Gower Street, London, WC1E 6BT, UK.
authors.forename: Ilias
authors.initials: I
authors.lastname: Tachtsidis
mesh_terms: D000818:Animals; D001921:Brain / Q000503:physiopathology; D003198:Computer Simulation; D020925:Hypoxia-Ischemia, Brain / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D019265:Spectroscopy, Near-Infrared / Q000379:methods*; D013552:Swine
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