title: Selection of functional human sperm with higher DNA integrity and fewer reactive oxygen species.
abstract: Fertilization and reproduction are central to the survival and propagation of a species. Couples who cannot reproduce naturally have to undergo in vitro clinical procedures. An integral part of these clinical procedures includes isolation of healthy sperm from raw semen. Existing sperm sorting methods are not efficient and isolate sperm having high DNA fragmentation and reactive oxygen species (ROS), and suffer from multiple manual steps and variations between operators. Inspired by in vivo natural sperm sorting mechanisms where vaginal mucus becomes less viscous to form microchannels to guide sperm towards egg, a chip is presented that efficiently sorts healthy, motile and morphologically normal sperm without centrifugation. Higher percentage of sorted sperm show significantly lesser ROS and DNA fragmentation than the conventional swim-up method. The presented chip is an easy-to-use high-throughput sperm sorter that provides standardized sperm sorting assay with less reliance on operators's skills, facilitating reliable operational steps.
authors.affiliation: Bio-Acoustic-MEMS in Medicine (BAMM) Laboratory, Division of Biomedical Engineering, Division of Infectious Diseases, Renal Division, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
authors.forename: Waseem
authors.initials: W
authors.lastname: Asghar
authors.forename: Vanessa
authors.initials: V
authors.lastname: Velasco
authors.forename: James L
authors.initials: JL
authors.lastname: Kingsley
authors.forename: Muhammad S
authors.initials: MS
authors.lastname: Shoukat
authors.forename: Hadi
authors.initials: H
authors.lastname: Shafiee
authors.forename: Raymond M
authors.initials: RM
authors.lastname: Anchan
authors.forename: George L
authors.initials: GL
authors.lastname: Mutter
authors.forename: Erkan
authors.initials: E
authors.lastname: Tüzel
authors.forename: Utkan
authors.initials: U
authors.lastname: Demirci
mesh_terms: D002469:Cell Separation / Q000379:methods*; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D053938:DNA Fragmentation; D006801:Humans; D008297:Male; D017382:Reactive Oxygen Species / Q000032:analysis* / Q000378:metabolism; D027724:Reproductive Techniques, Assisted; D013094:Spermatozoa / Q000166:cytology* / Q000378:metabolism / Q000502:physiology
chemical_list: D017382:Reactive Oxygen Species; D004247:DNA
keywords: DNA integrity; advance reproductive technologies; reactive oxygen species; reproduction; sperm sorting
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