title: Acetaldehyde targets superoxide dismutase 2 in liver cancer cells inducing transient enzyme impairment and a rapid transcriptional recovery.
abstract: Alcohol is undoubtedly, the main toxic agent that people consume by recreation and the abuse is associated with liver damage, mainly by the overproduction of reactive oxygen species and the toxic effects of its first metabolite acetaldehyde. It is known that acetaldehyde targets mitochondria inducing redox imbalance and oxidative stress. Mitochondrial superoxide dismutase transforms superoxide radical into hydrogen peroxide, which in addition, is transformed in water by other enzymes. In the present study we demonstrate that acetaldehyde transiently impairs SOD2 activity in HepG2 cells, the decrease in the enzyme activity was associated to a reduction in the protein content, which was rapidly recovered, to basal values, by synthesis de novo in a mechanism mediated by NF-κB and PKC. The SOD2 impairment was not associated with adduct formation. The recovery on SOD2 activity in HepG2 cells can represent survival advantage for cancer cells, the results shown that SOD2 could be considered a therapeutic target in liver cancer.
authors.affiliation: Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana Iztapalapa, México, DF, Mexico.
authors.forename: Denise
authors.initials: D
authors.lastname: Clavijo-Cornejo
authors.affiliation: Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana Iztapalapa, México, DF, Mexico.
authors.forename: Mario
authors.initials: M
authors.lastname: Gutiérrez-Carrera
authors.affiliation: Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana Iztapalapa, México, DF, Mexico.
authors.forename: Mayrel
authors.initials: M
authors.lastname: Palestino-Domínguez
authors.affiliation: Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana Iztapalapa, México, DF, Mexico.
authors.forename: Mayra
authors.initials: M
authors.lastname: Dominguez-Perez
authors.affiliation: Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana Iztapalapa, México, DF, Mexico.
authors.forename: Natalia
authors.initials: N
authors.lastname: Nuño
authors.affiliation: Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana Iztapalapa, México, DF, Mexico; Red Fisiopatología de las Enfermedades Hepáticas, PROMEP-SEP, México, DF, Mexico.
authors.forename: Veronica
authors.initials: V
authors.lastname: Souza
authors.affiliation: Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana Iztapalapa, México, DF, Mexico.
authors.forename: Roxana U
authors.initials: RU
authors.lastname: Miranda
authors.affiliation: Red Fisiopatología de las Enfermedades Hepáticas, PROMEP-SEP, México, DF, Mexico; Instituto Nacional de Ciencias Médicas y de la Nutrición Salvador Zubirán, México, DF, Mexico.
authors.forename: David
authors.initials: D
authors.lastname: Kershenobich
authors.affiliation: Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana Iztapalapa, México, DF, Mexico; Red Fisiopatología de las Enfermedades Hepáticas, PROMEP-SEP, México, DF, Mexico.
authors.forename: Ma Concepción
authors.initials: MC
authors.lastname: Gutiérrez-Ruiz
authors.affiliation: Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana Iztapalapa, México, DF, Mexico; Red Fisiopatología de las Enfermedades Hepáticas, PROMEP-SEP, México, DF, Mexico.
authors.forename: Leticia
authors.initials: L
authors.lastname: Bucio
authors.affiliation: Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana Iztapalapa, México, DF, Mexico; Red Fisiopatología de las Enfermedades Hepáticas, PROMEP-SEP, México, DF, Mexico. Electronic address: legq@xanum.uam.mx.
authors.forename: Luis E
authors.initials: LE
authors.lastname: Gómez-Quiroz
mesh_terms: D000079:Acetaldehyde / Q000494:pharmacology*; D056945:Hep G2 Cells / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D058990:Molecular Targeted Therapy; D016328:NF-kappa B / Q000378:metabolism; D011493:Protein Kinase C / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism*; D000072105:Superoxide Dismutase-1; D000099156:Superoxide Dismutase 2
chemical_list: D000079:Acetaldehyde; D016328:NF-kappa B; D011493:Protein Kinase C; D017382:Reactive Oxygen Species; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1; D000099156:Superoxide Dismutase 2; C000606290:SOD1 protein, human
keywords: Acetaldehyde; Hepatocytes; Liver cancer; Nuclear factor kappa B; SOD