title: Alteration of the systemic and microcirculation by a single oral dose of flavan-3-ols.
abstract: Several systematic reviews have reported that flow mediated dilatation (FMD) was significantly increased in subjects after ingestion of chocolate that contains flavan-3-ols; however, the mechanisms responsible for this effect are not clear. In this study, we evaluated the effects of a single oral dose of flavan-3-ols on the systemic circulation and microcirculation in the cremaster muscle using intravital video microscopy in vivo. The cremaster muscle in rats was spread over a plastic chamber and a gastric tube was placed into the stomach. Blood flow in the cremasteric artery was determined using a laser Doppler flowmeter, while blood pressure and heart rate were measured by the tail-cuff method. Red blood cell velocity in arterioles and blood flow in the artery were significantly increased 5 min after the administration of 10 mg/kg flavan-3-ols compared with distilled water treatment. The number of capillaries recruited in the cremaster muscle was also significantly increased 15 min after treatment. Microscopic observation confirmed that increased shear stress on endothelial cells was maintained during the measurement period. The mean arterial blood pressure and heart rate were also significantly elevated soon after administration and returned to baseline before the end of the observation period. Plasma nitrate and nitrite levels, and NO phosphorylation of aortic tissue were significantly increased at 60 min after administration of flavan-3-ols. According to these results, a single oral dose of flavan-3-ols elevates blood pressure and flow transiently, and these effects induce NO production through increased shear stress on endothelial cells.
authors.affiliation: Department of Bio-Science and Engineering, Shibaura Institute of Technology, Fukasaku, Munumaku Saitama, Japan.
authors.forename: Kodai
authors.initials: K
authors.lastname: Ingawa
authors.affiliation: Department of Bio-Science and Engineering, Shibaura Institute of Technology, Fukasaku, Munumaku Saitama, Japan.
authors.forename: Nozomi
authors.initials: N
authors.lastname: Aruga
authors.affiliation: Department of Bio-Science and Engineering, Shibaura Institute of Technology, Fukasaku, Munumaku Saitama, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Matsumura
authors.affiliation: Department of Bio-Science and Engineering, Shibaura Institute of Technology, Fukasaku, Munumaku Saitama, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Shibata
authors.affiliation: Department of Bio-Science and Engineering, Shibaura Institute of Technology, Fukasaku, Munumaku Saitama, Japan.
authors.forename: Naomi
authors.initials: N
authors.lastname: Osakabe
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001011:Aorta / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D001783:Blood Flow Velocity / Q000187:drug effects; D001794:Blood Pressure / Q000187:drug effects* / Q000502:physiology; D015153:Blotting, Western; D005419:Flavonoids / Q000008:administration & dosage / Q000494:pharmacology*; D006339:Heart Rate / Q000187:drug effects* / Q000502:physiology; D017078:Laser-Doppler Flowmetry; D008297:Male; D008833:Microcirculation / Q000187:drug effects* / Q000502:physiology; D018715:Microscopy, Video; D018482:Muscle, Skeletal / Q000098:blood supply / Q000187:drug effects / Q000502:physiology; D009566:Nitrates / Q000097:blood; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism; D009573:Nitrites / Q000097:blood; D010766:Phosphorylation / Q000187:drug effects; D017208:Rats, Wistar
chemical_list: D005419:Flavonoids; D009566:Nitrates; D009573:Nitrites; C404987:flavan-3-ol; D052250:Nitric Oxide Synthase Type III; C496316:Nos3 protein, rat
references.citation: Hypertension. 2010 Jun;55(6):1398-405
references.pmid: 20404222
references.citation: Free Radic Biol Med. 2003 Apr 1;34(7):840-9
references.pmid: 12654472
references.citation: J Agric Food Chem. 1998 Feb 16;46(2):454-457
references.pmid: 10554262
references.citation: Antioxid Redox Signal. 2001 Dec;3(6):1023-39
references.pmid: 11813978
references.citation: Phytochemistry. 2002 Apr;59(7):749-58
references.pmid: 11909632
references.citation: Physiol Behav. 2013 Jun 13;118:152-8
references.pmid: 23688945
references.citation: J Nutr. 2001 Nov;131(11):2885-91
references.pmid: 11694613
references.citation: Eur J Pharmacol. 2012 Oct 5;692(1-3):52-60
references.pmid: 22796647
references.citation: Experientia. 1979 Nov 15;35(11):1476-7
references.pmid: 510489
references.citation: Proc Natl Acad Sci U S A. 2006 Jan 24;103(4):1024-9
references.pmid: 16418281
references.citation: BMJ. 2011 Aug 26;343:d4488
references.pmid: 21875885
references.citation: Int J Sports Med. 2013 May;34(5):409-14
references.pmid: 23041960
references.citation: J Physiol. 2004 Nov 15;561(Pt 1):1-25
references.pmid: 15375191
references.citation: Exp Physiol. 2012 Mar;97(3):375-85
references.pmid: 22179420
references.citation: Anal Biochem. 1993 Oct;214(1):11-6
references.pmid: 7504409
references.citation: J Am Coll Cardiol. 2008 Jun 3;51(22):2141-9
references.pmid: 18510961
references.citation: J Nutr. 2011 Nov;141(11):1982-8
references.pmid: 21956956
references.citation: Circ Res. 2001 Nov 23;89(11):1073-80
references.pmid: 11717166
references.citation: J Agric Food Chem. 1999 Feb;47(2):490-6
references.pmid: 10563922
references.citation: Arch Intern Med. 2007 Apr 9;167(7):626-34
references.pmid: 17420419
references.citation: Am J Physiol Cell Physiol. 2011 Apr;300(4):C880-7
references.pmid: 21209365
references.citation: Cardiovasc Res. 2012 Nov 1;96(2):234-43
references.pmid: 22752349
references.citation: J Pharmacol Sci. 2011;116(4):323-31
references.pmid: 21757846
references.citation: J Nutr Sci Vitaminol (Tokyo). 1998 Apr;44(2):313-21
references.pmid: 9675711
references.citation: Eur J Appl Physiol. 2013 Feb;113(2):349-57
references.pmid: 22729610
references.citation: Am J Clin Nutr. 2012 Mar;95(3):740-51
references.pmid: 22301923
references.citation: Am J Clin Nutr. 2008 Jul;88(1):38-50
references.pmid: 18614722
references.citation: Am J Hypertens. 2010 Jan;23(1):97-103
references.pmid: 19910929
references.citation: BMC Med. 2010 Jun 28;8:39
references.pmid: 20584271
references.citation: Neurology. 2012 Sep 18;79(12):1223-9
references.pmid: 22933736
references.citation: J Appl Physiol (1985). 2011 Dec;111(6):1568-74
references.pmid: 21903881
references.citation: Biosci Biotechnol Biochem. 2000 Dec;64(12):2581-7
references.pmid: 11210120
references.citation: Eur J Clin Nutr. 2011 Aug;65(8):879-86
references.pmid: 21559039
references.citation: Am J Physiol. 1980 Jan;238(1):H31-42
references.pmid: 7356032
references.citation: Free Radic Biol Med. 2002 Jul 1;33(1):142-8
references.pmid: 12086692
references.citation: Eur J Nutr. 2011 Oct;50(7):595-9
references.pmid: 21327831