title: Enzymatic synthesis of γ-glutamylmethylamide from glutamic acid γ-methyl ester and methylamine catalyzed by Escherichia coli having γ-glutamyltranspeptidase activity.
abstract: A new method for the synthesis of γ-glutamylmethylamide is presented. Glutamic acid γ-methyl ester was used as substrate for γ-glutamylmethylamide synthesis catalyzed by Escherichia coli with γ-glutamyltranspeptidase activity. Reaction conditions were optimized by using 300 mM glutamic acid γ-methyl ester and 3,000 mM methylamine at pH 10 and 40 °C. Bioconversion rate of γ-glutamylmethylamide reached 87 % after 10 h. γ-Glutamyltranspeptidase was reversibly inhibited only when glutamic acid γ-methyl ester was above 300 mM.
authors.affiliation: Department of Chemistry and Life Science, Suzhou University, Suzhou, 234000, People's Republic of China, xulisheng111@163.com.
authors.forename: Lisheng
authors.initials: L
authors.lastname: Xu
authors.forename: Guizhen
authors.initials: G
authors.lastname: Gao
authors.forename: Cao
authors.initials: C
authors.lastname: Wengen
authors.forename: Jigui
authors.initials: J
authors.lastname: Xu
authors.forename: Liang
authors.initials: L
authors.lastname: Zhao
authors.forename: Hongwei
authors.initials: H
authors.lastname: Shi
authors.forename: Xingtao
authors.initials: X
authors.lastname: Zhang
mesh_terms: D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins / Q000737:chemistry*; D005971:Glutamates / Q000096:biosynthesis / Q000737:chemistry*; D008744:Methylamines / Q000737:chemistry*; D005723:gamma-Glutamyltransferase / Q000737:chemistry*
chemical_list: D029968:Escherichia coli Proteins; D005971:Glutamates; D008744:Methylamines; C014574:gamma-glutamylmethylamide; C027451:methylamine; D005723:gamma-Glutamyltransferase