title: Characterisation of Streptococcus suis isolates from wild boars (Sus scrofa).
abstract: Wild boar are widely distributed throughout the Iberian Peninsula and can carry potentially virulent strains of Streptococcus suis. The objective of this study was to determine the prevalence of S. suis in wild boars from two large geographical regions of Spain. Serotypes 1, 2, 7 and 9 identified were further genetically characterised by virulence-associated genotyping, pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) to determine the population structure of S. suis carried by these animals. Streptococcus suis was isolated from 39.1% of the wild boars examined: serotype 9 was the most frequently isolated (12.5%), followed by serotype 1 (2.5%). Serotype 2 was rarely isolated (0.3%). Eighteen additional serotypes were identified indicating wide diversity of this pathogen within the wild boar population. This heterogeneity was confirmed by PFGE and MLST analyses and the majority of isolates exhibited the virulence-associated genotype mrp-/epf-/sly-. The results of this study highlight that the carriage of S. suis by wild boars is commonplace. However, MLST data indicate that these isolates are not related to prevalent clonal complexes ST1, ST16, ST61 and ST87 typically associated with infection of pigs or humans in Europe.
authors.affiliation: Centro de Vigilancia Sanitaria Veterinaria (VISAVET), Universidad Complutense, 28040 Madrid, Spain.
authors.forename: Verónica
authors.initials: V
authors.lastname: Sánchez del Rey
authors.affiliation: Centro de Vigilancia Sanitaria Veterinaria (VISAVET), Universidad Complutense, 28040 Madrid, Spain; Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain.
authors.forename: José F
authors.initials: JF
authors.lastname: Fernández-Garayzábal
authors.affiliation: Servei d'Ecopatologia de Fauna Salvatge (SEFAS), Departament de Medicina i Cirurgia Animals, Facultat de Veterinària, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain.
authors.forename: Gregorio
authors.initials: G
authors.lastname: Mentaberre
authors.affiliation: Centro de Investigación en Sanidad Animal, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Ministerio de Economia y Competitividad, Valdeolmos, 28130 Madrid, Spain.
authors.forename: Víctor
authors.initials: V
authors.lastname: Briones
authors.affiliation: Servei d'Ecopatologia de Fauna Salvatge (SEFAS), Departament de Medicina i Cirurgia Animals, Facultat de Veterinària, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain.
authors.forename: Santiago
authors.initials: S
authors.lastname: Lavín
authors.affiliation: Centro de Vigilancia Sanitaria Veterinaria (VISAVET), Universidad Complutense, 28040 Madrid, Spain.
authors.forename: Lucas
authors.initials: L
authors.lastname: Domínguez
authors.affiliation: Groupe de Recherche sur les Maladies Infectieuses du Porc, Faculté de Médecine Vétérinaire, Université de Montréal, 3200 Sicotte, St.-Hyacinthe, QC, Canada J2S 2M2.
authors.forename: Marcelo
authors.initials: M
authors.lastname: Gottschalk
authors.affiliation: Centro de Vigilancia Sanitaria Veterinaria (VISAVET), Universidad Complutense, 28040 Madrid, Spain; Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain. Electronic address: avela@vet.ucm.es.
authors.forename: Ana Isabel
authors.initials: AI
authors.lastname: Vela
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field / Q000662:veterinary; D005838:Genotype; D008969:Molecular Sequence Data; D058885:Multilocus Sequence Typing / Q000662:veterinary; D010802:Phylogeny; D015995:Prevalence; D017422:Sequence Analysis, DNA / Q000662:veterinary; D013030:Spain / Q000453:epidemiology; D013290:Streptococcal Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D017011:Streptococcus suis / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology* / Q000382:microbiology; D014774:Virulence / Q000235:genetics
chemical_list: D001426:Bacterial Proteins
keywords: Molecular characterisation; Serotyping; Streptococcus suis; Wild boar