title: Antioxidant activities of two sericin proteins extracted from cocoon of silkworm (Bombyx mori) measured by DPPH, chemiluminescence, ORAC and ESR methods.
abstract: Recent efforts have focused on the use of sericin proteins extracted from cocoons of silkworm as a healthy food source for human consumption. In this study, we focused on the antioxidative properties of sericin proteins. The antioxidative properties were measured in sericin proteins extracted from the shell of the cocoon, designated hereafter as white sericin protein and yellow-green sericin protein, as well as bread without sericin protein and bread to which white sericin powder had been added using four measurement methods: 1,1-Diphenyl-2-picrylhydrazyl (DPPH), chemiluminescence, oxygen radical absorbance capacity (ORAC) and electron spin resonance (ESR). High antioxidative properties of sericin proteins were indicated by all four methods. A comparison of the two types of sericin proteins revealed that yellow-green sericin protein exhibited high antioxidative properties as indicated by the DPPH, chemiluminescence and ORAC methods. By contrast, a higher antioxidative property was determined in white sericin protein by the ESR method. Consequently, our findings confirmed that sericin proteins have antioxidative properties against multiple radicals. In addition, the antioxidative property of bread was enhanced by the addition of sericin powder to the bread. Therefore, findings of this study suggest that sericin proteins may be efficiently used as beneficial food for human health.
authors.affiliation: Faculty of Human Science, Kobe Shoin Women's University, Hyogo 657-0015, Japan ; Graduate School of Humanities and Science, Nara Women's University, Nara 630-8506, Japan.
authors.forename: Tayori
authors.initials: T
authors.lastname: Takechi
authors.affiliation: Laboratory of Marine Food Processing and Safety, Department of Food Science and Technology, National Fisheries University, Independent Administrative Institution, The Ministry of Agriculture Forestry and Fisheries of Japan, Yamaguchi 759-6595, Japan.
authors.forename: Ritsuko
authors.initials: R
authors.lastname: Wada
authors.affiliation: Laboratory of Marine Food Processing and Safety, Department of Food Science and Technology, National Fisheries University, Independent Administrative Institution, The Ministry of Agriculture Forestry and Fisheries of Japan, Yamaguchi 759-6595, Japan.
authors.forename: Tsubasa
authors.initials: T
authors.lastname: Fukuda
authors.affiliation: Laboratory of Marine Food Processing and Safety, Department of Food Science and Technology, National Fisheries University, Independent Administrative Institution, The Ministry of Agriculture Forestry and Fisheries of Japan, Yamaguchi 759-6595, Japan.
authors.forename: Kazuki
authors.initials: K
authors.lastname: Harada
authors.affiliation: Graduate School of Humanities and Science, Nara Women's University, Nara 630-8506, Japan ; KYOUSEI Science Center for Life and Nature, Nara Women's University, Nara 630-8506, Japan.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Takamura
keywords: 1,1-Diphenyl-2-picrylhydrazyl; antioxidant activity; chemiluminescence; electron spin resonance; oxygen radical absorbance capacity; resistant protein; sericin
references.citation: Mol Cell Biochem. 2008 Apr;311(1-2):111-9
references.pmid: 18214642
references.citation: Biosci Biotechnol Biochem. 1998 Jan;62(1):145-7
references.pmid: 9501526
references.citation: Pharm Biol. 2010 Aug;48(8):855-60
references.pmid: 20673171
references.citation: Int J Mol Med. 2003 Oct;12(4):621-5
references.pmid: 12964045
references.citation: Biomed Rep. 2013 May;1(3):355-358
references.pmid: 24648948
references.citation: BMB Rep. 2008 Mar 31;41(3):236-41
references.pmid: 18377728
references.citation: Mol Med Rep. 2010 Jul-Aug;3(4):663-8
references.pmid: 21472295
references.citation: Biosci Biotechnol Biochem. 2001 Oct;65(10):2181-6
references.pmid: 11758907
references.citation: Oncol Rep. 2003 May-Jun;10(3):537-43
references.pmid: 12684620
references.citation: Biosci Biotechnol Biochem. 1998 Jun;62(6):1201-4
references.pmid: 9692204
references.citation: Biosci Biotechnol Biochem. 2006 Dec;70(12):2844-52
references.pmid: 17151451
references.citation: J Biomater Sci Polym Ed. 2009;20(9):1295-306
references.pmid: 19520013
references.citation: J Hepatobiliary Pancreat Sci. 2012 Jul;19(4):354-60
references.pmid: 21678022
references.citation: Alcohol Alcohol. 2008 May-Jun;43(3):246-53
references.pmid: 18263900
references.citation: Chin Med J (Engl). 2008 Oct 20;121(20):2083-7
references.pmid: 19080280