title: Flexible microRNA arm selection in rice.
abstract: MicroRNAs act at the post-transcriptional level and guide Argonaute proteins to cleave their corresponding target transcripts. However, little attention has been paid to arm selection in miRNA precursors. In this study, small RNA high-throughput sequencing data from 29 different rice libraries were pooled to investigate tissue- and abiotic stress-specific dynamic expression of miRNAs. We found that more than half of pre-miRNAs showed changes in arm selection in different tissues and/or under different abiotic stresses. Our findings suggest that miRNA selection is remarkably prevalent in plants, providing new insights into the role of miRNAs in plant growth and development.
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, China; Institute of Crop Science, Zhejiang Key Laboratory of Crop Germplasm, Zhejiang University, Hangzhou, Zhejiang 310058, China; Zhejiang-California International Nanosystems Institute, Zhejiang University, Hangzhou, Zhejiang 310058, China.
authors.forename: Wangxiong
authors.initials: W
authors.lastname: Hu
authors.affiliation: Institute of Crop Science, Zhejiang Key Laboratory of Crop Germplasm, Zhejiang University, Hangzhou, Zhejiang 310058, China.
authors.forename: Tingzhang
authors.initials: T
authors.lastname: Wang
authors.affiliation: Institute of Crop Science, Zhejiang Key Laboratory of Crop Germplasm, Zhejiang University, Hangzhou, Zhejiang 310058, China.
authors.forename: Erkui
authors.initials: E
authors.lastname: Yue
authors.affiliation: Zhejiang-California International Nanosystems Institute, Zhejiang University, Hangzhou, Zhejiang 310058, China; Cancer Institute, Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang 310009, China. Electronic address: zhengshu@zju.edu.cn.
authors.forename: Shu
authors.initials: S
authors.lastname: Zheng
authors.affiliation: Institute of Crop Science, Zhejiang Key Laboratory of Crop Germplasm, Zhejiang University, Hangzhou, Zhejiang 310058, China. Electronic address: jhxu@zju.edu.cn.
authors.forename: Jian-Hong
authors.initials: JH
authors.lastname: Xu
mesh_terms: D060565:Argonaute Proteins / Q000235:genetics; D001483:Base Sequence; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D059014:High-Throughput Nucleotide Sequencing; D035683:MicroRNAs / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012275:Oryza / Q000235:genetics*; D012322:RNA Precursors / Q000737:chemistry / Q000235:genetics*; D018749:RNA, Plant / Q000737:chemistry / Q000235:genetics*; D012641:Selection, Genetic; D013312:Stress, Physiological
chemical_list: D060565:Argonaute Proteins; D035683:MicroRNAs; D012322:RNA Precursors; D018749:RNA, Plant
keywords: Abiotic stress; Arm switch; MicroRNA; pre-miRNA