title: Long-term monitoring of waterborne pathogens and microbial source tracking markers in paired agricultural watersheds under controlled and conventional tile drainage management.
abstract: Surface waters from paired agricultural watersheds under controlled tile drainage (CTD) and uncontrolled tile drainage (UCTD) were monitored over 7 years in order to determine if there was an effect of CTD (imposed during the growing season) on occurrences and loadings of bacterial and viral pathogens, coliphages, and microbial source tracking markers. There were significantly lower occurrences of human, ruminant, and livestock (ruminant plus pig) Bacteroidales markers in the CTD watershed in relation to the UCTD watershed. As for pathogens, there were significantly lower occurrences of Salmonella spp. and Arcobacter spp. in the CTD watershed. There were no instances where there were significantly higher quantitative loadings of any microbial target in the CTD watershed, except for F-specific DNA (F-DNA) and F-RNA coliphages, perhaps as a result of fecal inputs from a hobby farm independent of the drainage practice treatments. There was lower loading of the ruminant marker in the CTD watershed in relation to the UCTD system, and results were significant at the level P = 0.06. The odds of Salmonella spp. occurring increased when a ruminant marker was present relative to when the ruminant marker was absent, yet for Arcobacter spp., the odds of this pathogen occurring significantly decreased when a ruminant marker was present relative to when the ruminant marker was absent (but increased when a wildlife marker was present relative to when the wildlife marker was absent). Interestingly, the odds of norovirus GII (associated with human and swine) occurring in water increased significantly when a ruminant marker was present relative to when a ruminant marker was absent. Overall, this study suggests that fecal pollution from tile-drained fields to stream could be reduced by CTD utilization.
authors.affiliation: Agriculture and Agri-Food Canada, Ottawa, Ontario, Canada.
authors.forename: Graham
authors.initials: G
authors.lastname: Wilkes
authors.affiliation: Agriculture and Agri-Food Canada, Saint-Hyacinthe, Québec, Canada.
authors.forename: Julie
authors.initials: J
authors.lastname: Brassard
authors.affiliation: Water Science & Technology, Environment Canada, Burlington, Ontario, Canada.
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Edge
authors.affiliation: Laboratory for Foodborne Zoonoses, Public Health Agency of Canada, Lethbridge, Alberta, Canada.
authors.forename: Victor
authors.initials: V
authors.lastname: Gannon
authors.affiliation: Agriculture and Agri-Food Canada, Ottawa, Ontario, Canada.
authors.forename: Natalie
authors.initials: N
authors.lastname: Gottschall
authors.affiliation: Laboratory for Foodborne Zoonoses, Public Health Agency of Canada, Lethbridge, Alberta, Canada.
authors.forename: Cassandra C
authors.initials: CC
authors.lastname: Jokinen
authors.affiliation: Agriculture and Agri-Food Canada, Lacombe, Alberta, Canada.
authors.forename: Tineke H
authors.initials: TH
authors.lastname: Jones
authors.affiliation: Agriculture and Agri-Food Canada, Ottawa, Ontario, Canada.
authors.forename: Izhar U H
authors.initials: IU
authors.lastname: Khan
authors.affiliation: Agriculture and Agri-Food Canada, London, Ontario, Canada.
authors.forename: Romain
authors.initials: R
authors.lastname: Marti
authors.affiliation: Agriculture and Agri-Food Canada, Ottawa, Ontario, Canada.
authors.forename: Mark D
authors.initials: MD
authors.lastname: Sunohara
authors.affiliation: Agriculture and Agri-Food Canada, London, Ontario, Canada.
authors.forename: Edward
authors.initials: E
authors.lastname: Topp
authors.affiliation: Agriculture and Agri-Food Canada, Ottawa, Ontario, Canada David.Lapen@agr.gc.ca.
authors.forename: David R
authors.initials: DR
authors.lastname: Lapen
mesh_terms: D000383:Agriculture; D000818:Animals; D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification*; D015415:Biomarkers / Q000737:chemistry*; D004784:Environmental Monitoring; D006801:Humans; D045483:Rivers / Q000737:chemistry / Q000382:microbiology* / Q000821:virology*; D012621:Seasons; D014780:Viruses / Q000235:genetics / Q000302:isolation & purification*; D014871:Water Microbiology
chemical_list: D015415:Biomarkers
references.citation: J Microbiol Methods. 2013 Nov;95(2):201-6
references.pmid: 23999275
references.citation: J Gen Virol. 2007 Dec;88(Pt 12):3347-3359
references.pmid: 18024905
references.citation: Appl Environ Microbiol. 2003 Nov;69(11):6507-14
references.pmid: 14602607
references.citation: Water Res. 2013 Oct 15;47(16):6326-37
references.pmid: 24079968
references.citation: Emerg Infect Dis. 2007 Aug;13(8):1184-8
references.pmid: 17953089
references.citation: J Environ Qual. 2012 Jan-Feb;41(1):242-52
references.pmid: 22218192
references.citation: Water Res. 2013 Oct 15;47(16):6244-57
references.pmid: 24075721
references.citation: Can J Microbiol. 2007 Oct;53(10):1158-67
references.pmid: 18026208
references.citation: Water Res. 2013 Jun 15;47(10):3255-72
references.pmid: 23623467
references.citation: Appl Environ Microbiol. 2013 Oct;79(20):6207-19
references.pmid: 23913430
references.citation: Appl Environ Microbiol. 2007 Sep;73(17):5401-10
references.pmid: 17630309
references.citation: Water Res. 2013 May 1;47(7):2315-24
references.pmid: 23497974
references.citation: J Environ Qual. 2009 Mar 25;38(3):1066-75
references.pmid: 19329694
references.citation: J Environ Qual. 2013 May-Jun;42(3):713-25
references.pmid: 23673938
references.citation: J Environ Qual. 2014 Mar;43(2):617-30
references.pmid: 25602663
references.citation: Appl Environ Microbiol. 2013 Jan;79(2):434-48
references.pmid: 23124241
references.citation: J Environ Qual. 2008 May 02;37(3):925-36
references.pmid: 18453415
references.citation: J Environ Qual. 2002 Jan-Feb;31(1):300-8
references.pmid: 11841063
references.citation: Can J Microbiol. 1988 Jan;34(1):80-2
references.pmid: 3378202
references.citation: Water Res. 2009 May;43(8):2209-23
references.pmid: 19339033
references.citation: J Environ Qual. 2013 May-Jun;42(3):881-92
references.pmid: 23673956
references.citation: J Environ Qual. 2007 Jul 17;36(5):1324-30
references.pmid: 17636294
references.citation: Water Res. 2011 Nov 15;45(18):5807-25
references.pmid: 21889781
references.citation: Water Res. 2009 Nov;43(19):4838-49
references.pmid: 19604534
references.citation: Appl Environ Microbiol. 2000 May;66(5):1994-2000
references.pmid: 10788372
references.citation: J Environ Qual. 2007 Apr 05;36(3):718-29
references.pmid: 17412907