title: Monoolein production by triglycerides hydrolysis using immobilized Rhizopus oryzae lipase.
abstract: Lipase extracted from Rhizopus oryzae was immobilized in alginate gel beads. The effects of the immobilization conditions, such as, alginate concentration, CaCl2 concentration and amount of initial enzyme on retained activity (specific activity ratio of entrapped active lipase to free lipase) were investigated. The optimal conditions for lipase entrapment were determined: 2% (w/v) alginate concentration, 100mM CaCl2 and enzyme ratio of 2000IU/mL.In such conditions, immobilized lipase by inclusion in alginate showed a highest stability and activity, on olive oil hydrolysis reaction where it could be reused for 10 cycles. After 15min of hydrolysis reaction, the mass composition of monoolein, diolein and triolein were about 78%, 10% and 12%. Hydrolysis' products purification by column chromatography lead to a successful separation of reaction compounds and provide a pure fraction of monoolein which is considered as the widest used emulsifier in food and pharmaceutical industries.
authors.affiliation: Laboratory of Protein Engineering and Bioactive Molecules (LIP-MB), National Institute of Applied Sciences and Technology, University of Carthage, Centre Urbain Nord, B.P. 676, 1080 Tunis Cedex, Tunisia.
authors.forename: Nesrine
authors.initials: N
authors.lastname: Ghattas
authors.affiliation: Laboratory of Protein Engineering and Bioactive Molecules (LIP-MB), National Institute of Applied Sciences and Technology, University of Carthage, Centre Urbain Nord, B.P. 676, 1080 Tunis Cedex, Tunisia.
authors.forename: Ferid
authors.initials: F
authors.lastname: Abidi
authors.affiliation: Laboratory of Protein Engineering and Bioactive Molecules (LIP-MB), National Institute of Applied Sciences and Technology, University of Carthage, Centre Urbain Nord, B.P. 676, 1080 Tunis Cedex, Tunisia.
authors.forename: Said
authors.initials: S
authors.lastname: Galai
authors.affiliation: Laboratory of Protein Engineering and Bioactive Molecules (LIP-MB), National Institute of Applied Sciences and Technology, University of Carthage, Centre Urbain Nord, B.P. 676, 1080 Tunis Cedex, Tunisia.
authors.forename: M Nejib
authors.initials: MN
authors.lastname: Marzouki
authors.affiliation: Laboratory of Protein Engineering and Bioactive Molecules (LIP-MB), National Institute of Applied Sciences and Technology, University of Carthage, Centre Urbain Nord, B.P. 676, 1080 Tunis Cedex, Tunisia. Electronic address: bensalahraouf@yahoo.fr.
authors.forename: Abderraouf Ben
authors.initials: AB
authors.lastname: Salah
mesh_terms: D000464:Alginates / Q000494:pharmacology; D002119:Calcium Carbonate / Q000494:pharmacology; D004795:Enzyme Stability / Q000187:drug effects; D004800:Enzymes, Immobilized / Q000378:metabolism*; D020723:Glucuronic Acid / Q000494:pharmacology; D005989:Glycerides / Q000096:biosynthesis*; D006603:Hexuronic Acids / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D006868:Hydrolysis / Q000187:drug effects; D008049:Lipase / Q000378:metabolism*; D000069463:Olive Oil; D010938:Plant Oils / Q000494:pharmacology; D012233:Rhizopus / Q000201:enzymology*; D014280:Triglycerides / Q000378:metabolism*
chemical_list: D000464:Alginates; D004800:Enzymes, Immobilized; D005989:Glycerides; D006603:Hexuronic Acids; D000069463:Olive Oil; D010938:Plant Oils; D014280:Triglycerides; D020723:Glucuronic Acid; C005953:monoolein; D008049:Lipase; D002119:Calcium Carbonate
keywords: Alginate beads; Hydrolysis; Immobilization; Monoolein; Olive oil; Rhizopus oryzae