title: Sequential one-pot enzymatic synthesis of oligo-N-acetyllactosamine and its multi-sialylated extensions.
abstract: A simple and efficient protocol for the preparative-scale synthesis of various lengths of oligo-N-acetyllactosamine (oligo-LacNAc) and its multi-sialylated extensions is described. The strategy utilizes one thermophilic bacterial thymidylyltransferase (RmlA) coupled with corresponding sugar-1-phosphate kinases to generate two uridine diphosphate sugars, UDP-galactose and UDP-N-acetylglucosamine. By incorporating glycosyltransferases, oligo-LacNAcs and their sialylated analogs were synthesized.
authors.affiliation: Department of Chemistry, National Tsing Hua University, 101, Sec. 2, Kuang Fu Rd., Hsinchu, 30013, Taiwan. cclin66@mx.nthu.edu.tw.
authors.forename: Wei-Ting
authors.initials: WT
authors.lastname: Chien
authors.forename: Chien-Fu
authors.initials: CF
authors.lastname: Liang
authors.forename: Ching-Ching
authors.initials: CC
authors.lastname: Yu
authors.forename: Chien-Hung
authors.initials: CH
authors.lastname: Lin
authors.forename: Si-Peng
authors.initials: SP
authors.lastname: Li
authors.forename: Indah
authors.initials: I
authors.lastname: Primadona
authors.forename: Yu-Ju
authors.initials: YJ
authors.lastname: Chen
authors.forename: Kwok Kong T
authors.initials: KK
authors.lastname: Mong
authors.forename: Chun-Cheng
authors.initials: CC
authors.lastname: Lin
mesh_terms: D000606:Amino Sugars / Q000138:chemical synthesis* / Q000737:chemistry*; D060326:Chemistry Techniques, Synthetic; D016480:Helicobacter pylori / Q000201:enzymology; D017351:N-Acetylglucosaminyltransferases / Q000378:metabolism*; D009237:N-Acetyllactosamine Synthase / Q000378:metabolism*; D019158:N-Acetylneuraminic Acid / Q000737:chemistry*; D009345:Neisseria meningitidis / Q000201:enzymology
chemical_list: D000606:Amino Sugars; C000458:N-acetyllactosamine; D017351:N-Acetylglucosaminyltransferases; C037649:N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase; D009237:N-Acetyllactosamine Synthase; D019158:N-Acetylneuraminic Acid