title: BK virus encoded microRNAs are present in blood of renal transplant recipients with BK viral nephropathy.
abstract: BK viral infection is an important cause of renal transplant dysfunction and failure. Current strategies utilize surveillance for infection with DNA polymerase chain reaction assays and modulation of immunosuppression. Many viruses including polyomaviruses encode microRNAs (miRNAs). We have detected BK virus (BKV) encoded miRNAs in the blood of infected renal transplant recipients, and see a strong correlation between BKV encoded miRNA and BKV DNA in blood and a relationship between levels of bkv-miR-B1-5p and the presence of biopsy-proven BK viral nephropathy. Further research is needed to determine whether the detection of this and other virally encoded miRNAs may be useful in the diagnosis of active viral replication.
authors.affiliation: Department of Renal Medicine, Flinders Medical Centre, Adelaide, Australia; School of Medicine, Flinders University, Adelaide, Australia.
authors.forename: J Y Z
authors.initials: JY
authors.lastname: Li
authors.forename: K
authors.initials: K
authors.lastname: McNicholas
authors.forename: T Y
authors.initials: TY
authors.lastname: Yong
authors.forename: N
authors.initials: N
authors.lastname: Rao
authors.forename: P T H
authors.initials: PT
authors.lastname: Coates
authors.forename: G D
authors.initials: GD
authors.lastname: Higgins
authors.forename: R P
authors.initials: RP
authors.lastname: Carroll
authors.forename: R J
authors.initials: RJ
authors.lastname: Woodman
authors.forename: M Z
authors.initials: MZ
authors.lastname: Michael
authors.forename: J M
authors.initials: JM
authors.lastname: Gleadle
mesh_terms: D001739:BK Virus / Q000235:genetics* / Q000302:isolation & purification; D016022:Case-Control Studies; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D005500:Follow-Up Studies; D015967:Gene Expression Regulation, Viral; D006801:Humans; D007674:Kidney Diseases / Q000097:blood / Q000175:diagnosis* / Q000821:virology; D016030:Kidney Transplantation; D008297:Male; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D027601:Polyomavirus Infections / Q000097:blood / Q000175:diagnosis* / Q000821:virology; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000097:blood; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D066027:Transplant Recipients; D014779:Virus Replication
chemical_list: D004279:DNA, Viral; D035683:MicroRNAs; D012333:RNA, Messenger; D012367:RNA, Viral
keywords: BK nephropathy; BK virus; microRNA; renal transplant