title: Molecular characterization of Cryptosporidium spp. in children from Mexico.
abstract: Cryptosporidiosis is a parasitic disease caused by Cryptosporidium spp. In immunocompetent individuals, it usually causes an acute and self-limited diarrhea; in infants, infection with Cryptosporidium spp. can cause malnutrition and growth retardation, and declined cognitive ability. In this study, we described for the first time the distribution of C. parvum and C. hominis subtypes in 12 children in Mexico by sequence characterization of the 60-kDa glycoprotein (GP60) gene of Cryptosporidium. Altogether, 7 subtypes belonging to 4 subtype families of C. hominis (Ia, Ib, Id and Ie) and 1 subtype family of C. parvum (IIa) were detected, including IaA14R3, IaA15R3, IbA10G2, IdA17, IeA11G3T3, IIaA15G2R1 and IIaA16G1R1. The frequency of the subtype families and subtypes in the samples analyzed in this study differed from what was observed in other countries.
authors.affiliation: Departamento de Ciencias Químico Biológicas, Universidad de Sonora, Hermosillo, Sonora, México.
authors.forename: Olivia
authors.initials: O
authors.lastname: Valenzuela
authors.affiliation: Departamento de Ciencias Químico Biológicas, Universidad de Sonora, Hermosillo, Sonora, México.
authors.forename: Mariana
authors.initials: M
authors.lastname: González-Díaz
authors.affiliation: Departamento de Ciencias Químico Biológicas, Universidad de Sonora, Hermosillo, Sonora, México.
authors.forename: Adriana
authors.initials: A
authors.lastname: Garibay-Escobar
authors.affiliation: Departamento de Ciencias Químico Biológicas, Universidad de Sonora, Hermosillo, Sonora, México.
authors.forename: Alexel
authors.initials: A
authors.lastname: Burgara-Estrella
authors.affiliation: Servicio de Infectología, Hospital Infantil del Estado de Sonora, Hermosillo, Sonora, México.
authors.forename: Manuel
authors.initials: M
authors.lastname: Cano
authors.affiliation: Servicio de Infectología, Hospital Infantil del Estado de Sonora, Hermosillo, Sonora, México.
authors.forename: María
authors.initials: M
authors.lastname: Durazo
authors.affiliation: Laboratorio de Parasitología y Micología, Hospital Infantil de México Federico Gómez, México Distrito Federal, México.
authors.forename: Rosa M
authors.initials: RM
authors.lastname: Bernal
authors.affiliation: Laboratorio de Inmunologia, Centro de Investigación en Alimentación y Desarrollo A. C., Hermosillo, Sonora, México.
authors.forename: Jesús
authors.initials: J
authors.lastname: Hernandez
authors.affiliation: Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America.
authors.forename: Lihua
authors.initials: L
authors.lastname: Xiao
mesh_terms: D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D003457:Cryptosporidiosis / Q000469:parasitology*; D003458:Cryptosporidium / Q000145:classification / Q000235:genetics*; D005243:Feces / Q000469:parasitology; D005260:Female; D017125:Genes, Protozoan; D006023:Glycoproteins / Q000235:genetics*; D006801:Humans; D007223:Infant; D008297:Male; D008800:Mexico; D058889:Molecular Typing; D015800:Protozoan Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D006023:Glycoproteins; D015800:Protozoan Proteins
references.citation: Infect Genet Evol. 2007 Mar;7(2):197-205
references.pmid: 17010677
references.citation: Infect Genet Evol. 2012 Dec;12(8):1984-93
references.pmid: 22981927
references.citation: Parasitol Res. 2006 Sep;99(4):346-52
references.pmid: 16565813
references.citation: J Pediatr Hematol Oncol. 2011 Oct;33(7):570-2
references.pmid: 21941152
references.citation: Int J Parasitol. 2008 Jun;38(7):809-17
references.pmid: 18054936
references.citation: BMC Infect Dis. 2009 Sep 18;9:155
references.pmid: 19765310
references.citation: J Eukaryot Microbiol. 2006;53 Suppl 1:S24-5
references.pmid: 17169057
references.citation: Appl Environ Microbiol. 2011 Nov;77(21):7757-65
references.pmid: 21908628
references.citation: J Infect Dis. 2001 Feb 1;183(3):492-7
references.pmid: 11133382
references.citation: Biotechnol Adv. 2010 Jan-Feb;28(1):17-26
references.pmid: 19699288
references.citation: Vet Parasitol. 2003 Feb 13;111(2-3):153-9
references.pmid: 12531291
references.citation: Trans R Soc Trop Med Hyg. 2012 Mar;106(3):191-5
references.pmid: 22265078
references.citation: Acta Vet Scand. 1981;22(3-4):594-6
references.pmid: 6178277
references.citation: J Infect Dis. 2007 Sep 1;196(5):684-91
references.pmid: 17674309
references.citation: Infect Immun. 2002 Jul;70(7):3881-90
references.pmid: 12065532
references.citation: Parasitol Res. 2008 Nov;103(6):1347-53
references.pmid: 18709387
references.citation: Int J Parasitol. 2005 Apr 1;35(4):397-410
references.pmid: 15777916
references.citation: PLoS One. 2012;7(2):e31139
references.pmid: 22347442
references.citation: Exp Parasitol. 2010 Jan;124(1):80-9
references.pmid: 19358845
references.citation: J Clin Microbiol. 2003 Jun;41(6):2744-7
references.pmid: 12791920
references.citation: J Clin Microbiol. 2006 Dec;44(12):4303-8
references.pmid: 17005736
references.citation: Semin Pediatr Infect Dis. 2004 Oct;15(4):229-36
references.pmid: 15494946
references.citation: J Eukaryot Microbiol. 2001;Suppl:28S-31S
references.pmid: 11906067
references.citation: Salud Publica Mex. 2002 Jul-Aug;44(4):328-34
references.pmid: 12216520
references.citation: Semin Pediatr Infect Dis. 2004 Oct;15(4):253-9
references.pmid: 15494949
references.citation: Parasitol Res. 2006 Jan;98(2):150-2
references.pmid: 16328366
references.citation: J Infect Dis. 1983 May;147(5):824-8
references.pmid: 6842020
references.citation: Vet J. 2009 Mar;179(3):378-82
references.pmid: 18083583
references.citation: Ann Epidemiol. 2013 Nov;23(11):720-3
references.pmid: 23972618
references.citation: Vet Parasitol. 2004 Dec 9;126(1-2):37-56
references.pmid: 15567578
references.citation: J Clin Microbiol. 2008 Jul;46(7):2252-62
references.pmid: 18448696
references.citation: Trends Parasitol. 2006 Apr;22(4):160-7
references.pmid: 16503418
references.citation: Clin Microbiol Rev. 2004 Jan;17(1):72-97
references.pmid: 14726456
references.citation: Vet Parasitol. 2013 Mar 31;193(1-3):15-24
references.pmid: 23305974
references.citation: Exp Parasitol. 2008 Jan;118(1):118-21
references.pmid: 17618622
references.citation: Parasitology. 2007 Mar;134(Pt 3):351-8
references.pmid: 17076920
references.citation: Appl Environ Microbiol. 2010 Dec;76(24):8269-76
references.pmid: 20971867
references.citation: Curr Opin Infect Dis. 2004 Oct;17(5):483-90
references.pmid: 15353969
references.citation: J Infect Dev Ctries. 2007 Dec 01;1(3):242-56
references.pmid: 19734601
references.citation: Appl Environ Microbiol. 2012 Nov;78(22):8096-101
references.pmid: 22983961
references.citation: Lancet. 2002 Feb 16;359(9306):564-71
references.pmid: 11867110
references.citation: Br J Biomed Sci. 2001;58(1):7-10
references.pmid: 11284227
references.citation: Rev Invest Clin. 2000 Nov-Dec;52(6):625-31
references.pmid: 11256105
references.citation: Exp Parasitol. 2010 Aug;125(4):325-8
references.pmid: 20219461
references.citation: Trop Biomed. 2011 Aug;28(2):400-10
references.pmid: 22041762
references.citation: Am J Trop Med Hyg. 2006 Jul;75(1):78-82
references.pmid: 16837712
references.citation: Appl Environ Microbiol. 2010 Oct;76(19):6639-44
references.pmid: 20709840
references.citation: Emerg Infect Dis. 2001 Jan-Feb;7(1):49-56
references.pmid: 11266294
references.citation: Am J Trop Med Hyg. 2003 Apr;68(4):384-5
references.pmid: 12875284
references.citation: J Clin Microbiol. 2011 Mar;49(3):918-24
references.pmid: 21177904
references.citation: Trans R Soc Trop Med Hyg. 2011 Feb;105(2):102-8
references.pmid: 21093003
references.citation: Clinics (Sao Paulo). 2006 Apr;61(2):119-26
references.pmid: 16680328
references.citation: Infect Immun. 2000 Jul;68(7):4117-34
references.pmid: 10858229
references.citation: Exp Parasitol. 2011 Jan;127(1):42-5
references.pmid: 20599984
references.citation: Emerg Infect Dis. 1998 Oct-Dec;4(4):681-5
references.pmid: 9866750
references.citation: Exp Parasitol. 2009 Jun;122(2):124-7
references.pmid: 19233175
references.citation: Emerg Infect Dis. 1997 Oct-Dec;3(4):567-73
references.pmid: 9366611
references.citation: Parasitology. 2008 Sep;135(11):1263-70
references.pmid: 18664309
references.citation: Vet Parasitol. 2005 Nov 25;134(1-2):15-23
references.pmid: 16115735
references.citation: Emerg Infect Dis. 2008 Mar;14(3):496-8
references.pmid: 18325272
references.citation: Am J Epidemiol. 1998 Sep 1;148(5):497-506
references.pmid: 9737562
references.citation: Electrophoresis. 2008 Nov;29(20):4119-29
references.pmid: 18991263
references.citation: Vet Parasitol. 2010 May 11;169(3-4):387-90
references.pmid: 20149546
references.citation: J Clin Microbiol. 2005 Jun;43(6):2805-9
references.pmid: 15956401