title: The flanking sequence contributes to the immobilisation of spermine at the G-quadruplex in the NHE (nuclease hypersensitivity element) III1 of the c-Myc promoter.
abstract: Defining the molecular basis of the DNA sequence selectivity of polyamine binding is central to understanding polyamine-dependent gene expression. We have studied, by selective NMR experiments, the variation of spermine mobility and conformation in the presence of G-quadruplexes formed by sequences of the purine-rich strand of the c-Myc promoter, nuclease hypersensitivity element III1 (NHE III1). All the NHE quadruplexes restrict spermine mobility and induce a spermine conformational change but the most effective immobilisation occurs when all five G-tracts of the NHE III1 are present. This suggests structure within the nucleotides flanking the G-quadruplex has a role in immobilising spermine.
authors.affiliation: Research School of Chemistry, The Australian National University, Canberra, ACT 0200, Australia. Electronic address: max@rsc.anu.edu.au.
authors.forename: Max A
authors.initials: MA
authors.lastname: Keniry
authors.affiliation: Research School of Chemistry, The Australian National University, Canberra, ACT 0200, Australia.
authors.forename: Elisabeth A
authors.initials: EA
authors.lastname: Owen
mesh_terms: D002942:Circular Dichroism; D054856:G-Quadruplexes; D005786:Gene Expression Regulation; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D009154:Mutation; D009841:Oligonucleotides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011485:Protein Binding; D011487:Protein Conformation; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics*; D013096:Spermine / Q000737:chemistry* / Q000378:metabolism
chemical_list: D009841:Oligonucleotides; D016271:Proto-Oncogene Proteins c-myc; D013096:Spermine
keywords: DNA recognition; NMR spectroscopy; Quadruplex DNA; Spermine; c-Myc regulation