title: Structural characterization of natural ideal 6-O-sulfated agarose from red alga Gloiopeltis furcata.
abstract: A charge and size uniform polysaccharide GW2M was extracted with cold water from red alga Gloiopeltis furcata and purified by strong anion ion-exchange and gel permeation chromatography. Its chemical structure was identified by methylation, (1)H-(1)H COSY, (1)H-(13)C HMQC and (1)H-(13)C HMBC techniques. The experimental data showed that GW2M was composed of galactose (40.3%), 3,6-anhydro-galactose (34.1%) and sulfate (24.8%) with an average molecular mass of 20.6 kDa. The results proved GW2M was a linear repeating sequence of alternating (1→3)-linked 6-O-sulfated-β-d-galactose (G6S) and (1→4)-linked 3,6-anhydro-α-l-galactose (A) which made it to be an ideal 6-O-sulfated-agarose. The sequences of serial oligosaccharides prepared by mild acid and reductive acid hydrolysis from GW2M were confirmed using electrospray collision induced dissociation tandem mass spectrometry (ES-CID-MS/MS) technique.
authors.affiliation: Shandong Provincial Key Laboratory of Glycoscience and Glycoengineering, Key Laboratory of Marine Drugs of Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China; College of Chemical Engineering, Hengshui University, Hengshui, Hebei 053000, China.
authors.forename: Yannan
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Shandong Provincial Key Laboratory of Glycoscience and Glycoengineering, Key Laboratory of Marine Drugs of Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China.
authors.forename: Guangli
authors.initials: G
authors.lastname: Yu
authors.affiliation: Shandong Provincial Key Laboratory of Glycoscience and Glycoengineering, Key Laboratory of Marine Drugs of Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China.
authors.forename: Xiaoliang
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Shandong Provincial Key Laboratory of Glycoscience and Glycoengineering, Key Laboratory of Marine Drugs of Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China.
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Shandong Provincial Key Laboratory of Glycoscience and Glycoengineering, Key Laboratory of Marine Drugs of Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China.
authors.forename: Xuechao
authors.initials: X
authors.lastname: Sun
authors.affiliation: Shandong Provincial Key Laboratory of Glycoscience and Glycoengineering, Key Laboratory of Marine Drugs of Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China.
authors.forename: Guangling
authors.initials: G
authors.lastname: Jiao
authors.affiliation: Shandong Provincial Key Laboratory of Glycoscience and Glycoengineering, Key Laboratory of Marine Drugs of Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Shandong Provincial Key Laboratory of Glycoscience and Glycoengineering, Key Laboratory of Marine Drugs of Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China; Glycosciences Laboratory, Faculty of Medicine, Imperial College London, Hammersmith Campus, London W12 0NN, United Kingdom.
authors.forename: Wengang
authors.initials: W
authors.lastname: Chai
mesh_terms: D002236:Carbohydrate Conformation; D019906:Nuclear Magnetic Resonance, Biomolecular; D000461:Rhodophyta / Q000378:metabolism*; D012685:Sepharose / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D012685:Sepharose