title: MALDI-TOF MS analysis of cellodextrins and xylo-oligosaccharides produced by hindgut homogenates of Reticulitermes santonensis.
abstract: Hindgut homogenates of the termite Reticulitermes santonensis were incubated with carboxymethyl cellulose (CMC), crystalline celluloses or xylan substrates. Hydrolysates were analyzed with matrix-assisted laser desorption/ionization coupled to time-of-flight mass spectrometry (MALDI-TOF MS). The method was first set up using acid hydrolysis analysis to characterize non-enzymatic profiles. Commercial enzymes of Trichoderma reesei or T. longibrachiatum were also tested to validate the enzymatic hydrolysis analysis. For CMC hydrolysis, data processing and visual display were optimized to obtain comprehensive profiles and allow rapid comparison and evaluation of enzymatic selectivity, according to the number of substituents of each hydrolysis product. Oligosaccharides with degrees of polymerization (DPs) ranging from three to 12 were measured from CMC and the enzymatic selectivity was demonstrated. Neutral and acidic xylo-oligosaccharides with DPs ranging from three to 11 were measured from xylan substrate. These results are of interest for lignocellulose biomass valorization and demonstrated the potential of termites and their symbiotic microbiota as a source of interesting enzymes for oligosaccharides production.
authors.affiliation: Mass Spectrometry Laboratory, Department of Chemistry, University of Liège, Allée du Six Aout, B6c, Sart-Tilman, B-4000 Liège, Belgium. catherine.brasseur@ulg.ac.be.
authors.forename: Catherine
authors.initials: C
authors.lastname: Brasseur
authors.affiliation: Department of Functional and Evolutionary Entomology, Gembloux Agro-Bio Tech, University of Liège, 2 Passage des Déportés, B-5030 Gembloux, Belgium. julien.bauwens@ulg.ac.be.
authors.forename: Julien
authors.initials: J
authors.lastname: Bauwens
authors.affiliation: Department of Bio-Industries, Gembloux Agro-Bio Tech, University of Liège, 2 Passage des Déportés, B-5030 Gembloux, Belgium. cedric.tarayre@ulg.ac.be.
authors.forename: Cédric
authors.initials: C
authors.lastname: Tarayre
authors.affiliation: Department of Microbiology and Genomics, Gembloux Agro-Bio Tech, University of Liège, 6 Avenue Maréchal Juin, B-5030 Gembloux, Belgium. matteochris@netscape.be.
authors.forename: Christel
authors.initials: C
authors.lastname: Mattéotti
authors.affiliation: Department of Bio-Industries, Gembloux Agro-Bio Tech, University of Liège, 2 Passage des Déportés, B-5030 Gembloux, Belgium. p.thonart@ulg.ac.be.
authors.forename: Philippe
authors.initials: P
authors.lastname: Thonart
authors.affiliation: Department of Bio-Industries, Gembloux Agro-Bio Tech, University of Liège, 2 Passage des Déportés, B-5030 Gembloux, Belgium. j.destain@ulg.ac.be.
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Destain
authors.affiliation: Department of Functional and Evolutionary Entomology, Gembloux Agro-Bio Tech, University of Liège, 2 Passage des Déportés, B-5030 Gembloux, Belgium. frederic.francis@ulg.ac.be.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Francis
authors.affiliation: Department of Functional and Evolutionary Entomology, Gembloux Agro-Bio Tech, University of Liège, 2 Passage des Déportés, B-5030 Gembloux, Belgium. e.haubruge@ulg.ac.be.
authors.forename: Eric
authors.initials: E
authors.lastname: Haubruge
authors.affiliation: Department of Microbiology and Genomics, Gembloux Agro-Bio Tech, University of Liège, 6 Avenue Maréchal Juin, B-5030 Gembloux, Belgium. daniel.portetelle@ulg.ac.be.
authors.forename: Daniel
authors.initials: D
authors.lastname: Portetelle
authors.affiliation: Department of Microbiology and Genomics, Gembloux Agro-Bio Tech, University of Liège, 6 Avenue Maréchal Juin, B-5030 Gembloux, Belgium. m.vandenbol@ulg.ac.be.
authors.forename: Micheline
authors.initials: M
authors.lastname: Vandenbol
authors.affiliation: Organic and Biological Analytical Chemistry, Department of Chemistry, University of Liège, Allée du Six Aout, B6c, Sart-Tilman, B-4000 Liège, Belgium. jf.focant@ulg.ac.be.
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Focant
authors.affiliation: Mass Spectrometry Laboratory, Department of Chemistry, University of Liège, Allée du Six Aout, B6c, Sart-Tilman, B-4000 Liège, Belgium. e.depauw@ulg.ac.be.
authors.forename: Edwin
authors.initials: E
authors.lastname: De Pauw
mesh_terms: D000818:Animals; D002266:Carboxymethylcellulose Sodium / Q000737:chemistry; D002482:Cellulose / Q000031:analogs & derivatives* / Q000737:chemistry; D045424:Complex Mixtures / Q000737:chemistry; D003912:Dextrins / Q000737:chemistry*; D005656:Fungal Proteins / Q000737:chemistry; D006868:Hydrolysis; D019476:Insect Proteins / Q000737:chemistry; D007422:Intestines / Q000737:chemistry* / Q000201:enzymology; D020049:Isoptera / Q000737:chemistry* / Q000201:enzymology; D009844:Oligosaccharides / Q000737:chemistry*; D014242:Trichoderma / Q000737:chemistry / Q000201:enzymology; D014990:Xylans / Q000737:chemistry
chemical_list: D045424:Complex Mixtures; D003912:Dextrins; D005656:Fungal Proteins; D019476:Insect Proteins; D009844:Oligosaccharides; D014990:Xylans; D002482:Cellulose; C068994:cellodextrin; D002266:Carboxymethylcellulose Sodium
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