title: Identification of pummelo cultivars by using a panel of 25 selected SNPs and 12 DNA segments.
abstract: Pummelo cultivars are usually difficult to identify morphologically, especially when fruits are unavailable. The problem was addressed in this study with the use of two methods: high resolution melting analysis of SNPs and sequencing of DNA segments. In the first method, a set of 25 SNPs with high polymorphic information content were selected from SNPs predicted by analyzing ESTs and sequenced DNA segments. High resolution melting analysis was then used to genotype 260 accessions including 55 from Myanmar, and 178 different genotypes were thus identified. A total of 99 cultivars were assigned to 86 different genotypes since the known somatic mutants were identical to their original genotypes at the analyzed SNP loci. The Myanmar samples were genotypically different from each other and from all other samples, indicating they were derived from sexual propagation. Statistical analysis showed that the set of SNPs was powerful enough for identifying at least 1000 pummelo genotypes, though the discrimination power varied in different pummelo groups and populations. In the second method, 12 genomic DNA segments of 24 representative pummelo accessions were sequenced. Analysis of the sequences revealed the existence of a high haplotype polymorphism in pummelo, and statistical analysis showed that the segments could be used as genetic barcodes that should be informative enough to allow reliable identification of 1200 pummelo cultivars. The high level of haplotype diversity and an apparent population structure shown by DNA segments and by SNP genotypes, respectively, were discussed in relation to the origin and domestication of the pummelo species.
authors.affiliation: College of Horticulture and Landscape Architecture, Southwest University, Chongqing, China; Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture, Guangzhou, China; Institution of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Wu
authors.affiliation: Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture, Guangzhou, China.
authors.forename: Guang-yan
authors.initials: GY
authors.lastname: Zhong
authors.affiliation: Institute of Tropical and Subtropical Cash Crops, Yunnan Academy of Agricultural Science, Dehong, Yunnan, China.
authors.forename: Jian-qiang
authors.initials: JQ
authors.lastname: Yue
authors.affiliation: College of Horticulture and Landscape Architecture, Southwest University, Chongqing, China.
authors.forename: Run-ting
authors.initials: RT
authors.lastname: Yang
authors.affiliation: College of Horticulture and Landscape Architecture, Southwest University, Chongqing, China.
authors.forename: Chong
authors.initials: C
authors.lastname: Li
authors.affiliation: Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture, Guangzhou, China; Institution of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
authors.forename: Yue-jia
authors.initials: YJ
authors.lastname: Li
authors.affiliation: Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture, Guangzhou, China; Institution of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhong
authors.affiliation: College of Horticulture and Landscape Architecture, Southwest University, Chongqing, China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Wang
authors.affiliation: Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture, Guangzhou, China; Institution of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Jiang
authors.affiliation: Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture, Guangzhou, China; Institution of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
authors.forename: Ji-wu
authors.initials: JW
authors.lastname: Zeng
authors.affiliation: Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture, Guangzhou, China; Institution of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: College of Horticulture and Landscape Architecture, Southwest University, Chongqing, China.
authors.forename: Shu-tang
authors.initials: ST
authors.lastname: Yan
authors.affiliation: College of Horticulture and Landscape Architecture, Southwest University, Chongqing, China.
authors.forename: Xue-jun
authors.initials: XJ
authors.lastname: Bei
authors.affiliation: Institute of Tropical and Subtropical Cash Crops, Yunnan Academy of Agricultural Science, Dehong, Yunnan, China.
authors.forename: Dong-guo
authors.initials: DG
authors.lastname: Zhou
mesh_terms: D001483:Base Sequence; D002957:Citrus / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics*; D060146:Ecotype; D014644:Genetic Variation; D060005:Genotyping Techniques; D006239:Haplotypes / Q000235:genetics; D009691:Nucleic Acid Denaturation / Q000235:genetics; D009711:Nucleotides / Q000235:genetics; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011157:Population Dynamics
chemical_list: D018744:DNA, Plant; D009711:Nucleotides
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