title: The age and genomic integrity of neurons after cortical stroke in humans.
abstract: It has been unclear whether ischemic stroke induces neurogenesis or neuronal DNA rearrangements in the human neocortex. Using immunohistochemistry; transcriptome, genome and ploidy analyses; and determination of nuclear bomb test-derived (14)C concentration in neuronal DNA, we found neither to be the case. A large proportion of cortical neurons displayed DNA fragmentation and DNA repair a short time after stroke, whereas neurons at chronic stages after stroke showed DNA integrity, demonstrating the relevance of an intact genome for survival.
authors.affiliation: 1] Department of Cell and Molecular Biology, Karolinska Institute, Stockholm, Sweden. [2] Department of Neurology, University of Erlangen-Nuremberg, Erlangen, Germany. [3].
authors.forename: Hagen B
authors.initials: HB
authors.lastname: Huttner
authors.affiliation: 1] Department of Cell and Molecular Biology, Karolinska Institute, Stockholm, Sweden. [2].
authors.forename: Olaf
authors.initials: O
authors.lastname: Bergmann
authors.affiliation: Department of Physics and Astronomy, Ion Physics, Uppsala University, Sweden.
authors.forename: Mehran
authors.initials: M
authors.lastname: Salehpour
authors.affiliation: Department of Neurology, University of Erlangen-Nuremberg, Erlangen, Germany.
authors.forename: Attila
authors.initials: A
authors.lastname: Rácz
authors.affiliation: Lund Stem Cell Center, University Hospital, Lund, Sweden.
authors.forename: Jemal
authors.initials: J
authors.lastname: Tatarishvili
authors.affiliation: Department of Cell and Molecular Biology, Karolinska Institute, Stockholm, Sweden.
authors.forename: Emma
authors.initials: E
authors.lastname: Lindgren
authors.affiliation: Department of Neuropathology, Institute of Pathology, University of Debrecen, Medical and Health Science Centre, Debrecen, Hungary.
authors.forename: Tamás
authors.initials: T
authors.lastname: Csonka
authors.affiliation: Department of Neurology, University of Debrecen, Medical and Health Science Centre, Debrecen, Hungary.
authors.forename: László
authors.initials: L
authors.lastname: Csiba
authors.affiliation: Department of Neuropathology, Institute of Pathology, University of Debrecen, Medical and Health Science Centre, Debrecen, Hungary.
authors.forename: Tibor
authors.initials: T
authors.lastname: Hortobágyi
authors.affiliation: Department of Neuropathology, Institute of Pathology, University of Debrecen, Medical and Health Science Centre, Debrecen, Hungary.
authors.forename: Gábor
authors.initials: G
authors.lastname: Méhes
authors.affiliation: Department of Pathology, University Hospital, Lund, Sweden.
authors.forename: Elisabet
authors.initials: E
authors.lastname: Englund
authors.affiliation: Department of Gene Technology, Royal Institute of Technology, SciLifeLab, Stockholm, Sweden.
authors.forename: Beata Werne
authors.initials: BW
authors.lastname: Solnestam
authors.affiliation: Department of Cell and Molecular Biology, Karolinska Institute, Stockholm, Sweden.
authors.forename: Sofia
authors.initials: S
authors.lastname: Zdunek
authors.affiliation: 1] Center for Hematology and Regenerative Medicine, Karolinska University Hospital, Huddinge, Sweden. [2] Department of Medicine, Skaraborgs Sjukhus Skövde, Sweden.
authors.forename: Christian
authors.initials: C
authors.lastname: Scharenberg
authors.affiliation: Department of Cell and Molecular Biology, Karolinska Institute, Stockholm, Sweden.
authors.forename: Lena
authors.initials: L
authors.lastname: Ström
authors.affiliation: Department of Cell and Molecular Biology, Karolinska Institute, Stockholm, Sweden.
authors.forename: Patrik
authors.initials: P
authors.lastname: Ståhl
authors.affiliation: Department of Gene Technology, Royal Institute of Technology, SciLifeLab, Stockholm, Sweden.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Sigurgeirsson
authors.affiliation: Deep Sequencing Group, SFB655, Biotechnology Center, TU-Dresden, Dresden, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Dahl
authors.affiliation: Department of Neurology, University of Erlangen-Nuremberg, Erlangen, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Schwab
authors.affiliation: Department of Physics and Astronomy, Ion Physics, Uppsala University, Sweden.
authors.forename: Göran
authors.initials: G
authors.lastname: Possnert
authors.affiliation: Department of Mathematics, Institut Camille Jordan, Université de Lyon, Villeurbanne cedex, France.
authors.forename: Samuel
authors.initials: S
authors.lastname: Bernard
authors.affiliation: Lund Stem Cell Center, University Hospital, Lund, Sweden.
authors.forename: Zaal
authors.initials: Z
authors.lastname: Kokaia
authors.affiliation: Lund Stem Cell Center, University Hospital, Lund, Sweden.
authors.forename: Olle
authors.initials: O
authors.lastname: Lindvall
authors.affiliation: Department of Gene Technology, Royal Institute of Technology, SciLifeLab, Stockholm, Sweden.
authors.forename: Joakim
authors.initials: J
authors.lastname: Lundeberg
authors.affiliation: Department of Cell and Molecular Biology, Karolinska Institute, Stockholm, Sweden.
authors.forename: Jonas
authors.initials: J
authors.lastname: Frisén
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D016922:Cellular Senescence / Q000502:physiology*; D053938:DNA Fragmentation; D004260:DNA Repair / Q000502:physiology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D019579:Neocortex / Q000473:pathology* / Q000502:physiology; D009474:Neurons / Q000473:pathology* / Q000502:physiology; D020521:Stroke / Q000235:genetics* / Q000473:pathology*
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