title: A comparison study on the interactions of two oligosaccharides with tobacco cells by time-resolved fluorometric method.
abstract: The interactions of tobacco cells with two oceanic oligosaccharides, chitosan oligosaccharide (COS) and alginate derived oligosaccharide (AOS) were investigated and compared by time-resolved fluorometric method using two Eu(3+) complexes as luminescence probes. The binding processes of two oligosaccharides and tobacco leaf cells (epidermal and stomatal guard cells) were characterized by the luminescence imaging method based on the reaction of tobacco cells with oligosaccharide (COS or AOS) conjugated to a highly fluorescent Eu(3+) complex. In addition, the concentration changes of indole-3-acetic acid (IAA) and IAA-related peroxidase produced in the cells during the interaction of oligosaccharides (COS or AOS) and tobacco cells were also determined. The results indicate that the bioactivity of COS, with faster binding rate to the tobacco cells and stronger effect on the IAA generation in the cells, is remarkably higher than that of AOS. The comparison study on bioactivities of the two oligosaccharides reveals the relationship between structures and bioactivities of oligosaccharide, and suggests that COS should be more favorable to be used as a promotion reagent of plant growth in agriculture field.
authors.affiliation: School of Life Science and Technology, Dalian Ocean University, Dalian 116023, China.
authors.forename: Weihua
authors.initials: W
authors.lastname: Guo
authors.affiliation: Department of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors.forename: Heng
authors.initials: H
authors.lastname: Yin
authors.affiliation: State Key Laboratory of Fine Chemicals, Department of Chemistry, Dalian University of Technology, Dalian 116024, China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Ye
authors.affiliation: Department of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Fine Chemicals, Department of Chemistry, Dalian University of Technology, Dalian 116024, China.
authors.forename: Jingli
authors.initials: J
authors.lastname: Yuan
authors.affiliation: Department of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors.forename: Yuguang
authors.initials: Y
authors.lastname: Du
mesh_terms: D015200:Fluoroimmunoassay / Q000379:methods; D009844:Oligosaccharides / Q000032:analysis* / Q000378:metabolism*; D014026:Nicotiana / Q000166:cytology* / Q000378:metabolism*
chemical_list: D009844:Oligosaccharides