title: Production and in vivo antioxidant activity of Zn, Ge, Se-enriched mycelia by Cordyceps sinensis SU-01.
abstract: Cordyceps sinensis, a traditionally edible and medicinal fungus in China, cannot be artificially solid-cultured. Zinc (Zn), germanium (Ge), and selenium (Se) are the essential trace elements for human body. In this work, C. sinensis SU-01 was cultivated in liquid medium simultaneously containing Zn, Ge, and Se. The bioactive ingredients and in vivo antioxidant activities of Zn, Ge, Se-enriched mycelia (ZGSM) of C. sinensis SU-01 were investigated. Under the determined conditions, the Zn, Ge, and Se contents of ZGSM were 2543.16 ± 158.92, 1873.85 ± 81.82, and 1260.16 ± 107.12 μg/g, respectively. The optimal concentrations of Zn, Ge, and Se had a positive effect on biosynthesis of protein, polysaccharide, cordycepic acid, and amino acids. The activities of glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) of mice blood were 3.72 ± 0.15 and 28.74 ± 2.53 % higher than that of control, respectively, and the content of malondialdehyde (MDA) was 41.01 ± 3.66 % lower than that of control.
authors.affiliation: College of Life Science, Shandong Agricultural University, Taian, Shandong, 271018, People's Republic of China.
authors.forename: Lan
authors.initials: L
authors.lastname: Zheng
authors.forename: Long
authors.initials: L
authors.lastname: Hao
authors.forename: Hua
authors.initials: H
authors.lastname: Ma
authors.forename: Chengye
authors.initials: C
authors.lastname: Tian
authors.forename: Tong
authors.initials: T
authors.lastname: Li
authors.forename: Xinyi
authors.initials: X
authors.lastname: Sun
authors.forename: Mengshi
authors.initials: M
authors.lastname: Jia
authors.forename: Le
authors.initials: L
authors.lastname: Jia
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000032:analysis*; D001774:Blood Chemical Analysis; D032661:Cordyceps / Q000254:growth & development / Q000378:metabolism*; D004032:Diet / Q000379:methods*; D005857:Germanium / Q000378:metabolism*; D051379:Mice; D025282:Mycelium / Q000235:genetics / Q000378:metabolism*; D012643:Selenium / Q000378:metabolism*; D015032:Zinc / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D005857:Germanium; D012643:Selenium; D015032:Zinc
references.citation: Mycologia. 2005 Jan-Feb;97(1):130-8
references.pmid: 16389964
references.citation: J Agric Food Chem. 2006 May 3;54(9):3440-4
references.pmid: 16637705
references.citation: Acta Pol Pharm. 2007 Jan-Feb;64(1):45-51
references.pmid: 17665850
references.citation: Biochem Biophys Res Commun. 2005 Dec 9;338(1):668-76
references.pmid: 16126168
references.citation: Anal Biochem. 1987 Mar;161(2):559-66
references.pmid: 3034103
references.citation: Biomed Environ Sci. 1997 Sep;10(2-3):340-5
references.pmid: 9315328
references.citation: Mol Cell Biochem. 2003 May;247(1-2):37-44
references.pmid: 12841629
references.citation: J Biol Chem. 1997 Jul 18;272(29):18104-10
references.pmid: 9218443
references.citation: Lipids. 2009 Jul;44(7):643-54
references.pmid: 19504140
references.citation: Biol Trace Elem Res. 2009 Oct;131(1):81-9
references.pmid: 19252827
references.citation: Proc Natl Acad Sci U S A. 1996 Feb 6;93(3):1006-11
references.pmid: 8577704
references.citation: Endocrinology. 1996 Jul;137(7):3021-5
references.pmid: 8770927
references.citation: Methods Enzymol. 1995;252:38-53
references.pmid: 7476373
references.citation: Nature. 1991 Sep 19;353(6341):273-6
references.pmid: 1832744
references.citation: J Med Food. 2007 Mar;10(1):175-83
references.pmid: 17472484