title: Development and characterization of novel SSR markers in Siniperca kneri Garman.
abstract: In this study, 37 transcriptome-derived simple sequence repeat (SSR) markers and 18 genomic SSR markers were developed and characterized in the Chinese perch, Siniperca kneri Garman. The average allele number per locus was 5.1 (range: 2-8) for transcriptome-derived SSRs and 3.8 (range: 2-5) for genomic SSRs. The average observed and expected heterozygosities were 0.666 (range: 0.000-1.000) and 0.692 (range: 0.230-0.857) for transcriptome-derived SSRs, respectively. These values were 0.380 (range: 0.000-1.000) and 0.527 (range: 0.201-0.799) for genomic SSRs, respectively. The average polymorphic information content was 0.638 (range: 0.215-0.824) for transcriptome-derived SSRs and 0.477 (range: 0.183-0.752) for genomic SSRs. Seven of these loci exhibited departure from Hardy-Weinberg equilibrium after sequential Bonferroni's correction for multiple tests, and no significant deviation was observed for the linkage disequilibrium. These developed and characterized markers are anticipated to be useful for studies on population genetics, conservation genetics, and the fishery management of this species.
authors.affiliation: Key Lab of Freshwater Animal Breeding, College of Fisheries, Huazhong Agricultural University, Ministry of Agriculture, Wuhan, Hubei, China.
authors.forename: M
authors.initials: M
authors.lastname: Yang
authors.affiliation: Key Lab of Freshwater Animal Breeding, College of Fisheries, Huazhong Agricultural University, Ministry of Agriculture, Wuhan, Hubei, China.
authors.forename: H Z
authors.initials: HZ
authors.lastname: Zheng
authors.affiliation: Key Lab of Freshwater Animal Breeding, College of Fisheries, Huazhong Agricultural University, Ministry of Agriculture, Wuhan, Hubei, China.
authors.forename: X-F
authors.initials: XF
authors.lastname: Liang
authors.affiliation: Key Lab of Freshwater Animal Breeding, College of Fisheries, Huazhong Agricultural University, Ministry of Agriculture, Wuhan, Hubei, China.
authors.forename: C X
authors.initials: CX
authors.lastname: Tian
authors.affiliation: Key Lab of Freshwater Animal Breeding, College of Fisheries, Huazhong Agricultural University, Ministry of Agriculture, Wuhan, Hubei, China.
authors.forename: Y Q
authors.initials: YQ
authors.lastname: Dou
authors.affiliation: Key Lab of Freshwater Animal Breeding, College of Fisheries, Huazhong Agricultural University, Ministry of Agriculture, Wuhan, Hubei, China.
authors.forename: K C
authors.initials: KC
authors.lastname: Zhu
authors.affiliation: Key Lab of Freshwater Animal Breeding, College of Fisheries, Huazhong Agricultural University, Ministry of Agriculture, Wuhan, Hubei, China.
authors.forename: Y C
authors.initials: YC
authors.lastname: Yuan
mesh_terms: D000483:Alleles; D000818:Animals; D005260:Female; D056426:Genetic Loci; D005838:Genotype; D008297:Male; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D059372:Nucleotide Motifs; D010471:Perches / Q000235:genetics*; D011110:Polymorphism, Genetic; D059467:Transcriptome