title: Extraction of polysaccharides from a species of Chlorella.
abstract: The objective of this project was to devise a method to recover the total, water-soluble cell-wall polysaccharides of Chlorella. It was found that substantial quantities of polysaccharides could be extracted after treatment of the cells with a mildly acidic solution of sodium chlorite (yield of recovered polysaccharide, 19-22%). Water-soluble (13-19% yield) and 2% NaOH-soluble (3-6% yield) fractions were obtained. A second treatment gave a total yield of water-soluble polysaccharides of 24-25%, while reducing the amount of material soluble in 2% NaOH to 0.3%. Each polysaccharide fraction was composed of 6 different neutral sugars (rhamnose, arabinose, xylose, mannose, galactose, and glucose). There was evidence of the presence of uronic acid in all fractions. An alkaline hydrogen peroxide treatment of the cells resulted in a 19% yield of polysaccharides that were not further examined.
authors.affiliation: Whistler Center for Carbohydrate Research, Department of Food Science, Purdue University, West Lafayette, IN 47907, USA.
authors.forename: Zhongquan
authors.initials: Z
authors.lastname: Sui
authors.affiliation: Miami Valley Laboratories, Procter & Gamble Co., Cincinnati, OH 45252, USA.
authors.forename: Yonas
authors.initials: Y
authors.lastname: Gizaw
authors.affiliation: Whistler Center for Carbohydrate Research, Department of Food Science, Purdue University, West Lafayette, IN 47907, USA.
authors.forename: James N
authors.initials: JN
authors.lastname: BeMiller
mesh_terms: D002473:Cell Wall / Q000737:chemistry*; D005591:Chemical Fractionation; D002708:Chlorella / Q000737:chemistry* / Q000166:cytology; D002712:Chlorides / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000737:chemistry; D016877:Oxidants / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification*; D012972:Sodium Hydroxide / Q000737:chemistry; D012995:Solubility; D014574:Uronic Acids / Q000032:analysis; D014867:Water / Q000737:chemistry
chemical_list: D002712:Chlorides; D016877:Oxidants; D011134:Polysaccharides; D014574:Uronic Acids; D014867:Water; D012972:Sodium Hydroxide; D006861:Hydrogen Peroxide; C001599:chlorite