title: Tracking protein turnover and degradation by microscopy: photo-switchable versus time-encoded fluorescent proteins.
abstract: Expanded fluorescent protein techniques employing photo-switchable and fluorescent timer proteins have become important tools in biological research. These tools allow researchers to address a major challenge in cell and developmental biology, namely obtaining kinetic information about the processes that determine the distribution and abundance of proteins in cells and tissues. This knowledge is often essential for the comprehensive understanding of a biological process, and/or required to determine the precise point of interference following an experimental perturbation.
authors.affiliation: Zentrum für Molekulare Biologie der Universität Heidelberg, Deutsches Krebsforschungszentrum, DKFZ-ZMBH Allianz, Im Neuenheimer Feld 282, 69120 Heidelberg, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Knop
authors.forename: Bruce A
authors.initials: BA
authors.lastname: Edgar
mesh_terms: D000818:Animals; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism*; D018613:Microscopy, Confocal; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D015027:Zebrafish
chemical_list: D008164:Luminescent Proteins; D020033:Protein Isoforms; D043562:Receptors, G-Protein-Coupled; D011993:Recombinant Fusion Proteins
keywords: fluorescent timer proteins; live cell microscopy; photo-switchable fluorescent proteins; protein dynamics
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