title: Histological analysis of SLC38A6 (SNAT6) expression in mouse brain shows selective expression in excitatory neurons with high expression in the synapses.
abstract: SLC38A6 is one of the newly found members of the solute carrier 38 family consisting of total 11 members, of which only 6 have been characterized so far. Being the only glutamine transporter family expressed in the brain, this family of proteins are most probably involved in the regulation of the glutamate-glutamine cycle, responsible for preventing excitotoxicity. We used immunohistochemistry to show that SLC38A6 is primarily expressed in excitatory neurons and is not expressed in the astrocytes. Using proximity ligation assay, we have quantified the interactions of this SLC38 family protein with other proteins with known localization in the cells, showing that this transporter is expressed at the synapses. Moreover, this study has enabled us to come up with a model suggesting sub-cellular localization of SLC38A6 at the synaptic membrane of the excitatory neurons.
authors.affiliation: Department of Neuroscience, Functional Pharmacology, Uppsala University, Uppsala, Sweden.
authors.forename: Sonchita
authors.initials: S
authors.lastname: Bagchi
authors.affiliation: Department of Neuroscience, Functional Pharmacology, Uppsala University, Uppsala, Sweden.
authors.forename: Hajar Ali
authors.initials: HA
authors.lastname: Baomar
authors.affiliation: Department of Neuroscience, Functional Pharmacology, Uppsala University, Uppsala, Sweden.
authors.forename: Somar
authors.initials: S
authors.lastname: Al-Walai
authors.affiliation: Department of Neuroscience, Functional Pharmacology, Uppsala University, Uppsala, Sweden.
authors.forename: Saifaddin
authors.initials: S
authors.lastname: Al-Sadi
authors.affiliation: Department of Neuroscience, Functional Pharmacology, Uppsala University, Uppsala, Sweden.
authors.forename: Robert
authors.initials: R
authors.lastname: Fredriksson
mesh_terms: D026921:Amino Acid Transport Systems, Neutral / Q000378:metabolism*; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001921:Brain / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D015180:Electrophoresis, Gel, Two-Dimensional; D004622:Embryo, Mammalian / Q000166:cytology / Q000378:metabolism; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D008810:Mice, Inbred C57BL; D008856:Microscopy, Fluorescence; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009457:Neuroglia / Q000378:metabolism; D009474:Neurons / Q000378:metabolism*; D016612:Paraffin Embedding; D025941:Protein Interaction Mapping; D022142:Secretory Vesicles / Q000378:metabolism; D013569:Synapses / Q000378:metabolism*
chemical_list: D026921:Amino Acid Transport Systems, Neutral; D015415:Biomarkers; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; C000594369:SNAT6 protein, mouse
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