title: Affinity proteomics reveals elevated muscle proteins in plasma of children with cerebral malaria.
abstract: Systemic inflammation and sequestration of parasitized erythrocytes are central processes in the pathophysiology of severe Plasmodium falciparum childhood malaria. However, it is still not understood why some children are more at risks to develop malaria complications than others. To identify human proteins in plasma related to childhood malaria syndromes, multiplex antibody suspension bead arrays were employed. Out of the 1,015 proteins analyzed in plasma from more than 700 children, 41 differed between malaria infected children and community controls, whereas 13 discriminated uncomplicated malaria from severe malaria syndromes. Markers of oxidative stress were found related to severe malaria anemia while markers of endothelial activation, platelet adhesion and muscular damage were identified in relation to children with cerebral malaria. These findings suggest the presence of generalized vascular inflammation, vascular wall modulations, activation of endothelium and unbalanced glucose metabolism in severe malaria. The increased levels of specific muscle proteins in plasma implicate potential muscle damage and microvasculature lesions during the course of cerebral malaria.
authors.affiliation: SciLifeLab Stockholm, School of Biotechnology, KTH-Royal Institute of Technology, Stockholm, Sweden.
authors.forename: Julie
authors.initials: J
authors.lastname: Bachmann
authors.affiliation: Division of Parasitology, Medical Research Council National Institute for Medical Research, London, United Kingdom.
authors.forename: Florence
authors.initials: F
authors.lastname: Burté
authors.affiliation: Department of Medical Epidemiology and Biostatistics, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Setia
authors.initials: S
authors.lastname: Pramana
authors.affiliation: Division of Parasitology, Medical Research Council National Institute for Medical Research, London, United Kingdom.
authors.forename: Ianina
authors.initials: I
authors.lastname: Conte
authors.affiliation: Department of Paediatrics, College of Medicine, University of Ibadan, University College Hospital, Ibadan, Nigeria; Department of Haematology, College of Medicine, University of Ibadan, University College Hospital, Ibadan, Nigeria; Childhood Malaria Research Group, University College Hospital, Ibadan, Nigeria.
authors.forename: Biobele J
authors.initials: BJ
authors.lastname: Brown
authors.affiliation: Department of Paediatrics, College of Medicine, University of Ibadan, University College Hospital, Ibadan, Nigeria.
authors.forename: Adebola E
authors.initials: AE
authors.lastname: Orimadegun
authors.affiliation: Department of Paediatrics, College of Medicine, University of Ibadan, University College Hospital, Ibadan, Nigeria.
authors.forename: Wasiu A
authors.initials: WA
authors.lastname: Ajetunmobi
authors.affiliation: Department of Paediatrics, College of Medicine, University of Ibadan, University College Hospital, Ibadan, Nigeria.
authors.forename: Nathaniel K
authors.initials: NK
authors.lastname: Afolabi
authors.affiliation: Department of Paediatrics, College of Medicine, University of Ibadan, University College Hospital, Ibadan, Nigeria.
authors.forename: Francis
authors.initials: F
authors.lastname: Akinkunmi
authors.affiliation: Department of Paediatrics, College of Medicine, University of Ibadan, University College Hospital, Ibadan, Nigeria; Childhood Malaria Research Group, University College Hospital, Ibadan, Nigeria.
authors.forename: Samuel
authors.initials: S
authors.lastname: Omokhodion
authors.affiliation: Department of Paediatrics, College of Medicine, University of Ibadan, University College Hospital, Ibadan, Nigeria; Childhood Malaria Research Group, University College Hospital, Ibadan, Nigeria.
authors.forename: Felix O
authors.initials: FO
authors.lastname: Akinbami
authors.affiliation: Department of Haematology, College of Medicine, University of Ibadan, University College Hospital, Ibadan, Nigeria; Childhood Malaria Research Group, University College Hospital, Ibadan, Nigeria.
authors.forename: Wuraola A
authors.initials: WA
authors.lastname: Shokunbi
authors.affiliation: Department of Immunology, Genetics and Pathology, Rudbeck Laboratory, Uppsala University, Uppsala, Sweden.
authors.forename: Caroline
authors.initials: C
authors.lastname: Kampf
authors.affiliation: Department of Medical Epidemiology and Biostatistics, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Yudi
authors.initials: Y
authors.lastname: Pawitan
authors.affiliation: SciLifeLab Stockholm, School of Biotechnology, KTH-Royal Institute of Technology, Stockholm, Sweden.
authors.forename: Mathias
authors.initials: M
authors.lastname: Uhlén
authors.affiliation: Division of Parasitology, Medical Research Council National Institute for Medical Research, London, United Kingdom; Department of Paediatrics, College of Medicine, University of Ibadan, University College Hospital, Ibadan, Nigeria; Department of Haematology, College of Medicine, University of Ibadan, University College Hospital, Ibadan, Nigeria; Childhood Malaria Research Group, University College Hospital, Ibadan, Nigeria.
authors.forename: Olugbemiro
authors.initials: O
authors.lastname: Sodeinde
authors.affiliation: SciLifeLab Stockholm, School of Biotechnology, KTH-Royal Institute of Technology, Stockholm, Sweden.
authors.forename: Jochen M
authors.initials: JM
authors.lastname: Schwenk
authors.affiliation: Department of Microbiology, Tumour and Cell Biology, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Mats
authors.initials: M
authors.lastname: Wahlgren
authors.affiliation: Division of Parasitology, Medical Research Council National Institute for Medical Research, London, United Kingdom; Department of Paediatrics, College of Medicine, University of Ibadan, University College Hospital, Ibadan, Nigeria; Department of Haematology, College of Medicine, University of Ibadan, University College Hospital, Ibadan, Nigeria; Childhood Malaria Research Group, University College Hospital, Ibadan, Nigeria; Brighton & Sussex Medical School, Sussex University, Brighton, United Kingdom.
authors.forename: Delmiro
authors.initials: D
authors.lastname: Fernandez-Reyes
authors.affiliation: SciLifeLab Stockholm, School of Biotechnology, KTH-Royal Institute of Technology, Stockholm, Sweden.
authors.forename: Peter
authors.initials: P
authors.lastname: Nilsson
mesh_terms: D000293:Adolescent; D015415:Biomarkers / Q000097:blood; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D016779:Malaria, Cerebral / Q000097:blood*; D008297:Male; D018384:Oxidative Stress; D010963:Plasmodium falciparum; D040901:Proteomics / Q000379:methods*; D013577:Syndrome
chemical_list: D015415:Biomarkers
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