title: Physcomitrella patens has kinase-LRR R gene homologs and interacting proteins.
abstract: Plant disease resistance gene (R gene)-like sequences were screened from the Physcomitrella patens genome. We found 603 kinase-like, 475 Nucleotide Binding Site (NBS)-like and 8594 Leucine Rich Repeat (LRR)-like sequences by homology searching using the respective domains of PpC24 (Accession No. BAD38895), which is a candidate kinase-NBS-LRR (kinase-NL) type R-like gene, as a reference. The positions of these domains in the genome were compared and 17 kinase-NLs were predicted. We also found four TIR-NBS-LRR (TIR-NL) sequences with homology to Arabidopsis TIR-NL (NM_001125847), but three out of the four TIR-NLs had tetratricopeptide repeats or a zinc finger domain in their predicted C-terminus. We also searched for kinase-LRR (KLR) type sequences by homology with rice OsXa21 and Arabidopsis thaliana FLS2. As a result, 16 KLRs with similarity to OsXa21 were found. In phylogenetic analysis of these 16 KLRs, PpKLR36, PpKLR39, PpKLR40, and PpKLR43 formed a cluster with OsXa21. These four PpKLRs had deduced transmembrane domain sequences and expression of all four was confirmed. We also found 14 homologs of rice OsXB3, which is known to interact with OsXa21 and is involved in signal transduction. Protein-protein interaction was observed between the four PpKLRs and at least two of the XB3 homologs in Y2H analysis.
authors.affiliation: Department of Biotechnological Science, Kinki University, Wakayama, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Tanigaki
authors.affiliation: Department of Biotechnological Science, Kinki University, Wakayama, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Ito
authors.affiliation: Department of Biotechnological Science, Kinki University, Wakayama, Japan.
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Obuchi
authors.affiliation: Department of Biotechnological Science, Kinki University, Wakayama, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Kosaka
authors.affiliation: Department of Biotechnological Science, Kinki University, Wakayama, Japan.
authors.forename: Katsuyuki T
authors.initials: KT
authors.lastname: Yamato
authors.affiliation: Department of Biotechnological Science, Kinki University, Wakayama, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Okanami
authors.affiliation: Department of Agricultural Sciences, University of Helsinki, Helsinki, Finland.
authors.forename: Mikko T
authors.initials: MT
authors.lastname: Lehtonen
authors.affiliation: Department of Agricultural Sciences, University of Helsinki, Helsinki, Finland.
authors.forename: Jari P T
authors.initials: JP
authors.lastname: Valkonen
authors.affiliation: Department of Biotechnological Science, Kinki University, Wakayama, Japan.
authors.forename: Motomu
authors.initials: M
authors.lastname: Akita
mesh_terms: D000595:Amino Acid Sequence; D019068:Bryopsida / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D011494:Protein Kinases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
chemical_list: D010940:Plant Proteins; D011494:Protein Kinases
references.citation: Methods Enzymol. 1991;194:94-110
references.pmid: 2005827
references.citation: Mol Plant Microbe Interact. 2008 May;21(5):507-17
references.pmid: 18393610
references.citation: Dev Cell. 2008 Aug;15(2):220-35
references.pmid: 18694562
references.citation: Proc Natl Acad Sci U S A. 2006 Dec 5;103(49):18395-400
references.pmid: 17082309
references.citation: Plant Cell. 2006 Dec;18(12):3635-46
references.pmid: 17172358
references.citation: PLoS Pathog. 2013;9(4):e1003313
references.pmid: 23637603
references.citation: BMC Plant Biol. 2007 Oct 08;7:52
references.pmid: 17922917
references.citation: Plant Mol Biol. 2006 Jan;60(2):293-319
references.pmid: 16429265
references.citation: Plant J. 2011 Sep;67(5):929-40
references.pmid: 21595760
references.citation: Sci Total Environ. 2011 Aug 1;409(17):3166-73
references.pmid: 21624641
references.citation: New Phytol. 2009;183(2):432-443
references.pmid: 19453432
references.citation: Curr Opin Plant Biol. 2012 Aug;15(4):358-66
references.pmid: 22658367
references.citation: Science. 1993 Nov 26;262(5138):1432-6
references.pmid: 7902614
references.citation: PLoS One. 2011 Jan 27;6(1):e16608
references.pmid: 21304602
references.citation: Mol Cells. 2012 Apr;33(4):385-92
references.pmid: 22453776
references.citation: Proc Natl Acad Sci U S A. 2011 May 31;108(22):9286-91
references.pmid: 21576467
references.citation: Plant Cell. 2006 Feb;18(2):465-76
references.pmid: 16377758
references.citation: Science. 1995 Dec 15;270(5243):1804-6
references.pmid: 8525370
references.citation: J Mol Evol. 2002 Nov;55(5):595-605
references.pmid: 12399933
references.citation: Proc Natl Acad Sci U S A. 2010 Jul 27;107(30):13544-9
references.pmid: 20624958
references.citation: Science. 2008 Jan 4;319(5859):64-9
references.pmid: 18079367
references.citation: Cell. 1996 Apr 19;85(2):149-58
references.pmid: 8612268
references.citation: Proc Natl Acad Sci U S A. 2007 Dec 4;104(49):19613-8
references.pmid: 18042724
references.citation: Am J Bot. 2009 Jul;96(7):1281-8
references.pmid: 21628277
references.citation: J Biol Chem. 2005 Mar 11;280(10):9203-9
references.pmid: 15572355
references.citation: Mol Plant Microbe Interact. 2012 Mar;25(3):363-71
references.pmid: 22112216
references.citation: Nature. 2001 Jun 14;411(6839):826-33
references.pmid: 11459065
references.citation: J Biol Chem. 2002 Jun 7;277(23):20264-9
references.pmid: 11927577
references.citation: PLoS One. 2012;7(5):e36700
references.pmid: 22615795
references.citation: Annu Rev Plant Physiol Plant Mol Biol. 1997 Jun;48:575-607
references.pmid: 15012275