title: Ectopic expression of an Arabidopsis dehydration-responsive element-binding factor DREB2C improves salt stress tolerance in crucifers.
abstract: DREB2C acts as a transcriptional activator of the salt tolerance-related COLD - REGULATED 15A gene. DEHYDRATION-RESPONSIVE ELEMENT BINDING FACTOR 2C (DREB2C) regulates plant responses to heat stress. We report here that DREB2C is induced by NaCl stress in Arabidopsis, based on quantitative RT-PCR analyses of transcript levels and DREB2C promoter-controlled GUS activity assays. Constitutive overexpression of DREB2C from the cauliflower mosaic virus (CaMV) 35S promoter led to enhanced salt tolerance in transgenic Arabidopsis and canola plants that was characterized by higher chlorophyll content, lower tissue Na(+) content, reduced rate of water loss, and tighter membrane integrity in plants grown in NaCl-containing medium. Basal expression of the stress-responsive genes COLD-REGULATED 15A (COR15A), RESPONSIVE TO DEHYDRATION (RD) 29A and RD29B, was higher in transgenic DREB2C-overexpressing Arabidopsis plants than in the wild-type. Promoter transactivation assays and electrophoretic mobility-shift assays showed that DREB2C interacts directly with the three DREs in the COR15A promoter, both in vivo and in vitro. Transgenic Arabidopsis constitutively overexpressing COR15A from the CaMV35S promoter exhibited greater NaCl tolerance than the untransformed wild-type. Taken together, the data suggest that DREB2C functions as transcriptional activator that promotes NaCl tolerance, in part through upregulation of the stress-responsive gene COR15A.
authors.affiliation: Division of Applied Life Science, Systems and Synthetic Agrobiotech Center and PMBBRC, Gyeongsang National University, Jinju, 660-701, Korea.
authors.forename: Chieun
authors.initials: C
authors.lastname: Song
authors.forename: Jihyun
authors.initials: J
authors.lastname: Je
authors.forename: Joon Ki
authors.initials: JK
authors.lastname: Hong
authors.forename: Chae Oh
authors.initials: CO
authors.lastname: Lim
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D029688:Brassica napus / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D002734:Chlorophyll / Q000378:metabolism; D057773:Cold Shock Proteins and Peptides / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D024202:Electrophoretic Mobility Shift Assay; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D018515:Plant Leaves / Q000235:genetics / Q000502:physiology; D018517:Plant Roots / Q000235:genetics / Q000502:physiology; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding; D018749:RNA, Plant / Q000235:genetics; D012965:Sodium Chloride / Q000494:pharmacology*; D013312:Stress, Physiological; D014867:Water / Q000032:analysis
chemical_list: D029681:Arabidopsis Proteins; C116166:COR15 protein, Arabidopsis; D057773:Cold Shock Proteins and Peptides; D004268:DNA-Binding Proteins; C573537:DREB2C protein, Arabidopsis; C000595439:RD29B protein, Arabidopsis; C520629:RD29a protein, Arabidopsis; D018749:RNA, Plant; D014867:Water; D002734:Chlorophyll; D012965:Sodium Chloride
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