title: Neural stem cells and glioblastoma.
abstract: Glioblastoma multiforme represents one of the most common brain cancers with a rather heterogeneous cellular composition, as indicated by the term "multiforme". Recent reports have described the isolation and identification of cancer neural stem cells from human adult glioblastoma multiforme, which possess the capacity to establish, sustain, and expand these tumours, even under the challenging settings posed by serial transplantation experiments. Our study focused on the distribution of neural cancer stem cells inside the tumour. The study is divided into three phases: removal of tumoral specimens in different areas of the tumour (centre, periphery, marginal zone) in an operative room equipped with a 1.5 T scanner; isolation and characterization of neural cancer stem cells from human adult glioblastoma multiforme; identification of neural cancer stem cell distribution inside the tumour.
authors.affiliation: Department of Neurosurgery; "Santa Maria" Hospital Trust, Terni, Italy - rossella.rispoli@libero.it.
authors.forename: Rossella
authors.initials: R
authors.lastname: Rispoli
authors.affiliation: Department of Neurosurgery; "Santa Maria" Hospital Trust, Terni, Italy.
authors.forename: Carlo
authors.initials: C
authors.lastname: Conti
authors.affiliation: Department of Neurosurgery, Sant'Andrea Hospital; Rome, Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: Celli
authors.affiliation: Department of Neurosurgery, Sant'Andrea Hospital; Rome, Italy.
authors.forename: Emanuela
authors.initials: E
authors.lastname: Caroli
authors.affiliation: Department of Neurosurgery; "Santa Maria" Hospital Trust, Terni, Italy.
authors.forename: Sandro
authors.initials: S
authors.lastname: Carletti
mesh_terms: D000071916:AC133 Antigen; D000328:Adult; D015703:Antigens, CD / Q000378:metabolism; D001932:Brain Neoplasms / Q000378:metabolism / Q000473:pathology*; D005909:Glioblastoma / Q000378:metabolism / Q000473:pathology*; D006023:Glycoproteins / Q000378:metabolism; D006801:Humans; D021621:Imaging, Three-Dimensional; D007150:Immunohistochemistry; D008279:Magnetic Resonance Imaging; D014411:Neoplastic Stem Cells / Q000378:metabolism / Q000473:pathology*; D058953:Neural Stem Cells / Q000378:metabolism / Q000473:pathology*; D010455:Peptides / Q000378:metabolism; D018874:Spheroids, Cellular; D014407:Tumor Cells, Cultured
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D006023:Glycoproteins; D010455:Peptides
keywords: brainsuite; glioblastoma; neural stem cells
references.citation: J Neurosci. 1996 Feb 1;16(3):1091-100
references.pmid: 8558238
references.citation: Cancer Res. 2003 Sep 15;63(18):5821-8
references.pmid: 14522905
references.citation: Mol Cancer Res. 2006 Sep;4(9):607-19
references.pmid: 16966431
references.citation: Acta Neurochir (Wien). 1978;42(1-2):5-32
references.pmid: 211808
references.citation: Cancer Res. 2007 May 1;67(9):4010-5
references.pmid: 17483311
references.citation: Nat Rev Cancer. 2006 Jun;6(6):425-36
references.pmid: 16723989
references.citation: Cancer Res. 2004 Oct 1;64(19):7011-21
references.pmid: 15466194
references.citation: Glia. 2002 Sep;39(3):193-206
references.pmid: 12203386
references.citation: Stem Cells. 2000;18(4):295-300
references.pmid: 10924096
references.citation: Semin Oncol. 2004 Oct;31(5):635-44
references.pmid: 15497116