title: MTHFR polymorphisms C677T and A1298C and associations with IVF outcomes in Brazilian women.
abstract: The aim of this study was to investigate the association between MTHFR gene polymorphisms and IVF outcomes in Brazilian women undergoing assisted reproduction treatment. A prospective study was conducted in the Human Reproduction Department at the ABC University School of Medicine and the Ideia Fertility Institute between December 2010 and April 2012. The patient population was 82 women undergoing assisted reproduction cycles. The MTHFR polymorphisms C677T and A1298C were evaluated and compared with laboratory results and pregnancy rates. The C677T variant was associated with proportions of mature (P=0.006) and immature (P=0.003) oocytes whereas the A1298C variant was associated with number of oocytes retrieved (P=0.044). The polymorphisms, whether alone or in combination, were not associated with normal fertilization, good-quality embryo or clinical pregnancy rates. This study suggests that the number and maturity of oocytes retrieved may be related to the MTHFR polymorphisms C677T and A1298C. It is believed that folate has a crucial function in human reproduction and that folate deficiency can compromise the function of the metabolic pathways it is involved in, leading to an accumulation of homocysteine. The gene MTHFR encodes the 5-MTHFR enzyme, which is involved in folate metabolism, and C677T/A1298C polymorphisms of this gene are related to decreased enzyme activity and consequent changes in homocysteine concentration. Folate deficiency and hyperhomocysteinaemia can also compromise fertility and lead to pregnancy complications by affecting the development of oocytes, preparation of endometrial receptivity, implantation of the embryo and pregnancy. In folliculogenesis, hyperhomocysteinaemia can activate apoptosis, leading to follicular atresia and affecting the maturity of oocytes and the quality of embryos cultured in vitro. This study was performed to investigate the association between MTHFR polymorphisms and IVF outcomes in women undergoing assisted reproduction treatment.
authors.affiliation: Faculdade de Ciências Médicas da Santa Casa de São Paulo, São Paulo 01221-020, Brazil. Electronic address: priqueirozbiomedica@hotmail.com.
authors.forename: Priscila Queiroz
authors.initials: PQ
authors.lastname: D'Elia
authors.affiliation: Faculdade de Medicina do ABC, Santo André 09060-650, Brazil.
authors.forename: Aline Amaro
authors.initials: AA
authors.lastname: dos Santos
authors.affiliation: Faculdade de Medicina do ABC, Santo André 09060-650, Brazil; Instituto Ideia Fértil de Saúde Reprodutiva, Santo André 09060-650, Brazil.
authors.forename: Bianca
authors.initials: B
authors.lastname: Bianco
authors.affiliation: Faculdade de Medicina do ABC, Santo André 09060-650, Brazil; Instituto Ideia Fértil de Saúde Reprodutiva, Santo André 09060-650, Brazil.
authors.forename: Caio Parente
authors.initials: CP
authors.lastname: Barbosa
authors.affiliation: Faculdade de Medicina do ABC, Santo André 09060-650, Brazil; Instituto Ideia Fértil de Saúde Reprodutiva, Santo André 09060-650, Brazil.
authors.forename: Denise Maria
authors.initials: DM
authors.lastname: Christofolini
authors.affiliation: Faculdade de Ciências Médicas da Santa Casa de São Paulo, São Paulo 01221-020, Brazil.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Aoki
mesh_terms: D000328:Adult; D001938:Brazil; D005260:Female; D005307:Fertilization in Vitro; D005838:Genotype; D006801:Humans; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics*; D011110:Polymorphism, Genetic; D011247:Pregnancy; D018873:Pregnancy Rate; D016896:Treatment Outcome
chemical_list: D042965:Methylenetetrahydrofolate Reductase (NADPH2)
keywords: ICSI; MTHFR; assisted reproduction; embryos; genetic; polymorphism