title: Dynamics of WD-repeat containing proteins in SSU processome components.
abstract: Nine WD-repeat containing proteins in human SSU processome components have been found in a HeLa cell nuclear matrix fraction. In these proteins, t-UTP sub-complex components, i.e., CIRH1A, UTP15, and WDR43, were shown to be immobilized in the fibrillar centers of nucleoli in living cells. In this study, the dynamics of the remaining six proteins fused with green fluorescent protein (GFP), i.e., PWP2-GFP, TBL3-GFP, GFP-UTP18, GFP-NOL10, GFP-WDR46, and GFP-WDSOF1, were examined in living cells. The findings were as follows. (i) The majority of UTP-B sub-complex components, i.e., PWP2-GFP, TBL3-GFP, and GFP-UTP18, are localized to the dense fibrillar component and granular component regions in nucleoli; (ii) When rRNA transcription is suppressed, the majority of GFP-fused UTP-B sub-complex components are localized in the cap and body regions of nucleoli. (iii) The mobility of these proteins except for GFP-WDSOF1, and half of GFP-UTP18 and GFP-WDR46, respectively, is very low in living cells. (iv) When rRNA transcription is suppressed, the mobility of these proteins except for GFP-WDSOF1 is accelerated but still slow. These findings and others suggest that these WD-repeat proteins other than GFP-WDSOF1 found in the nuclear matrix fraction bind tightly to some macro-protein complexes and act as a scaffold or a core for the complexes in nucleoli.
authors.affiliation: a Graduate School of Science and Technology, Department of Chemistry, Faculty of Science, Niigata University, Igarashi-2, Nishi-ku, Niigata 950-2181, Japan.
authors.forename: Kouko
authors.initials: K
authors.lastname: Wada
authors.forename: Manae
authors.initials: M
authors.lastname: Sato
authors.forename: Nanase
authors.initials: N
authors.lastname: Araki
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kumeta
authors.forename: Yuya
authors.initials: Y
authors.lastname: Hirai
authors.forename: Kunio
authors.initials: K
authors.lastname: Takeyasu
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Furukawa
authors.forename: Tsuneyoshi
authors.initials: T
authors.lastname: Horigome
mesh_terms: D006367:HeLa Cells; D006801:Humans; D009687:Nuclear Proteins / Q000737:chemistry* / Q000378:metabolism*; D012323:RNA Processing, Post-Transcriptional; D012335:RNA, Ribosomal / Q000235:genetics / Q000378:metabolism; D012261:Ribonucleoproteins / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D009687:Nuclear Proteins; D012335:RNA, Ribosomal; D012261:Ribonucleoproteins
keywords: PWP2; TBL3; UTP18; WD-repeat; nucleolus; nucléole; répétition WD