title: Optimization of 2,3-butanediol production by Enterobacter cloacae in simultaneous saccharification and fermentation of corncob residue.
abstract: Corncob residue, a waste in xylose or xylitol production, was utilized to produce 2,3-butanediol (2,3-BD) via simultaneous saccharification and fermentation (SSF). This study developed the optimal conditions for production of 2,3-BD by using a heat-resistant strain, Enterobacter cloacae UV4, to perform SSF of the corncob residue. Urea, lactic acid, sodium citrate, and MgSO4 , selected by the Plackett-Burman experiment, were determined to be significant independent variables to conduct the response surface experiment. With the optimized medium, a total production of 28.923 g/L for 2,3-BD and acetoin (BA) was obtained at 60 H. Furthermore, 43.162 g/L of BA production and 0.553 g/L/H of productivity were obtained by fed-batch SSF, which was 0.424 g diol/g consumed corncob residue. The results suggest that the waste corncob residue could be used as an available substrate for the production of 2,3-BD by E. cloacae UV4, as well as a potential resource to improve the economics of microbial compound production.
authors.affiliation: Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin Industrial Microbiology Key Laboratory, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, People's Republic of China.
authors.forename: Cui-Ying
authors.initials: CY
authors.lastname: Zhang
authors.forename: Xiao-Pei
authors.initials: XP
authors.lastname: Peng
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Xue-Wu
authors.initials: XW
authors.lastname: Guo
authors.forename: Dong-Guang
authors.initials: DG
authors.lastname: Xiao
mesh_terms: D061249:Batch Cell Culture Techniques / Q000379:methods*; D056804:Biofuels; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology*; D002072:Butylene Glycols / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry / Q000378:metabolism; D016972:Enterobacter cloacae / Q000378:metabolism*; D005285:Fermentation; D003313:Zea mays / Q000737:chemistry*
chemical_list: D056804:Biofuels; D002072:Butylene Glycols; D003470:Culture Media; C026978:2,3-butylene glycol
keywords: 2,3-butanediol; Enterobacter cloacae; biofuels; corncob residue; fed-batch operation; fermentation