title: Toxicity Effect of Silver Nanoparticles on Mice Liver Primary Cell Culture and HepG2 Cell Line.
abstract: Nano-silver (AgNP) has biological properties which are significant for consumer products, food technology, textiles and medical applications (e.g. wound care products, implantable medical devices, in diagnosis, drug delivery, and imaging). For their antibacterial activity, silver nanoparticles are largely used in various commercially available products. Thus, the use of nano-silver is becoming more and more widespread in medicine. In this study we investigated the cytotoxic effects of AgNPs on liver primary cells of mice, as well as the human liver HepG2 cell. Cell viability was examined with MTT assay after HepG2 cells exposure to AgNPs at 1, 2, 3, 4, 5, 7.5, 10 ppm compared to mice primary liver cells at 1, 10, 50, 100, 150, 200, 400 ppm for 24h. AgNPs caused a concentration-dependent decrease of cell viability in both cells. IC50 value of 2.764 ppm (µg/mL) was calculated in HepG2 cell line and IC50 value of 121.7 ppm (µg/mL) was calculated in primary liver cells of mice. The results of this experiment indicated that silver nanoparticles had cytotoxic effects on HepG2 cell line and primary liver cells of mice. The results illustrated that nano-silver had 44 times stronger inhibitory effect on the growth of cancerous cells (HepG2 cell line) compared to the normal cells (primary liver cells of mice). which might further justify AgNPs as a cytotoxic agents and a potential anticancer candidate which needs further studies in this regard.
authors.affiliation: Department of Basic Science, Science and Research Branch, Islamic Azad University, Tehran, Iran.
authors.forename: Firouz
authors.initials: F
authors.lastname: Faedmaleki
authors.affiliation: Pharmaceutical Sciences Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Farshad
authors.initials: F
authors.lastname: H Shirazi
authors.affiliation: Deptartment of Toxicology , Aja University of Medical Sciense, Tehran, Iran.
authors.forename: Amir-Ahmad
authors.initials: AA
authors.lastname: Salarian
authors.affiliation: Islamic Azad University, Pharmaceutical Seines Branch, Tehran, Iran.
authors.forename: Hamidreza
authors.initials: H
authors.lastname: Ahmadi Ashtiani
authors.affiliation: Department of Basic Science, Science and Research Branch, Islamic Azad University, Tehran, Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Rastegar
keywords: Cell viability; HepG2 cell line; MTT assay; Nanotechnology; Primary liver cells of mice; Silver nanoparticle
references.citation: Arch Toxicol. 2011 Dec;85(12):1529-40
references.pmid: 21611810
references.citation: Environ Health Perspect. 2001 Aug;109 Suppl 4:547-51
references.pmid: 11544161
references.citation: Toxicol Sci. 2005 Dec;88(2):412-9
references.pmid: 16014736
references.citation: J Biomed Mater Res. 2000 Dec 15;52(4):662-8
references.pmid: 11033548
references.citation: Inhal Toxicol. 2007;19 Suppl 1:59-65
references.pmid: 17886052
references.citation: Toxicol In Vitro. 2005 Oct;19(7):975-83
references.pmid: 16125895
references.citation: Nat Nanotechnol. 2006 Oct;1(1):3-5
references.pmid: 18654126
references.citation: Iran J Pharm Res. 2013 Summer;12(3):511-9
references.pmid: 24250657
references.citation: J Phys Chem B. 2006 Oct 5;110(39):19220-5
references.pmid: 17004772
references.citation: Int Immunopharmacol. 2007 Dec 15;7(13):1813-8
references.pmid: 17996693
references.citation: J Toxicol Environ Health A. 2009;72(21-22):1279-84
references.pmid: 20077197
references.citation: Clin Chim Acta. 2010 Dec 14;411(23-24):1841-8
references.pmid: 20719239
references.citation: In Vitro Cell Dev Biol Anim. 1996 Apr;32(4):211-20
references.pmid: 8727045
references.citation: Toxicol Lett. 2008 Jan 4;176(1):1-12
references.pmid: 18022772
references.citation: Nat Biotechnol. 2003 Oct;21(10):1166-70
references.pmid: 14520401
references.citation: Toxicol In Vitro. 2011 Aug;25(5):1053-60
references.pmid: 21501681
references.citation: Toxicol Appl Pharmacol. 2009 May 1;236(3):310-8
references.pmid: 19269301
references.citation: J Nanosci Nanotechnol. 2007 Jun;7(6):1893-7
references.pmid: 17654961
references.citation: Science. 2006 Feb 3;311(5761):622-7
references.pmid: 16456071
references.citation: ACS Nano. 2009 Feb 24;3(2):279-90
references.pmid: 19236062
references.citation: J Phys Chem B. 2008 Oct 30;112(43):13608-19
references.pmid: 18831567
references.citation: Toxicol Sci. 2006 Aug;92(2):456-63
references.pmid: 16714391
references.citation: Chem Commun (Camb). 2007 Jul 28;(28):2959-61
references.pmid: 17622444
references.citation: J Trauma. 2006 Mar;60(3):648-52
references.pmid: 16531870
references.citation: Inhal Toxicol. 2007 Aug;19(10):857-71
references.pmid: 17687717
references.citation: Trends Biotechnol. 2010 Nov;28(11):580-8
references.pmid: 20724010
references.citation: Inhal Toxicol. 2008 Apr;20(6):567-74
references.pmid: 18444009
references.citation: Biomaterials. 2009 Aug;30(23-24):3891-914
references.pmid: 19427031
references.citation: Biomaterials. 2004 Aug;25(18):4383-91
references.pmid: 15046929
references.citation: Inhal Toxicol. 2008 Apr;20(6):575-83
references.pmid: 18444010
references.citation: Iran J Pharm Res. 2013 Summer;12(3):289-98
references.pmid: 24250635
references.citation: Toxicol In Vitro. 2009 Sep;23(6):1076-84
references.pmid: 19508889