title: Linear synthesis and conformational analysis of the pentasaccharide repeating unit of the cell wall O-antigen of Escherichia coli O13.
abstract: Synthesis of the pentasaccharide repeating unit of the O-antigen of Escherichia coli O13 strain has been achieved using a straightforward linear synthetic strategy. Similar reaction conditions have been used for all glycosylations as well as protective group manipulations. All intermediate steps are high yielding and the glycosylation steps are stereoselective. The synthesized pentasaccharide was subjected to conformational analysis using 2D ROESY NMR spectral analysis and molecular dynamics (MD) simulation to get detailed information on conformation of the molecule in aqueous solution.
authors.affiliation: Bose Institute, Division of Molecular Medicine, P-1/12, C.I.T. Scheme VII-M, Kolkata 700 054, India.
authors.forename: Abhishek
authors.initials: A
authors.lastname: Santra
authors.affiliation: Bose Institute, Division of Molecular Medicine, P-1/12, C.I.T. Scheme VII-M, Kolkata 700 054, India.
authors.forename: Anshupriya
authors.initials: A
authors.lastname: Si
authors.affiliation: Bose Institute, Department of Biophysics, P-1/12, C.I.T. Scheme VII-M, Kolkata 700 054, India.
authors.forename: Rajiv Kumar
authors.initials: RK
authors.lastname: Kar
authors.affiliation: Bose Institute, Department of Biophysics, P-1/12, C.I.T. Scheme VII-M, Kolkata 700 054, India.
authors.forename: Anirban
authors.initials: A
authors.lastname: Bhunia
authors.affiliation: Bose Institute, Division of Molecular Medicine, P-1/12, C.I.T. Scheme VII-M, Kolkata 700 054, India. Electronic address: akmisra69@gmail.com.
authors.forename: Anup Kumar
authors.initials: AK
authors.lastname: Misra
mesh_terms: D002236:Carbohydrate Conformation; D002473:Cell Wall / Q000737:chemistry*; D004926:Escherichia coli / Q000737:chemistry* / Q000166:cytology*; D019081:O Antigens / Q000737:chemistry*; D009844:Oligosaccharides / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D019081:O Antigens; D009844:Oligosaccharides
keywords: Conformational analysis; Escherichia coli; Molecular dynamics simulation; O-Antigen; Pentasaccharide