title: Bioprocessing of Stichococcus bacillaris strain siva2011.
abstract: BACKGROUND
  Globally, the development of a cost-effective long-term renewable energy infrastructure is one of the most challenging problems faced by society today. Microalgae are rich in potential biofuel substrates such as lipids, including triacylglycerols (TAGs). Some of these algae also biosynthesize small molecule hydrocarbons. These hydrocarbons can often be used as liquid fuels, often with more versatility and by a more direct approach than some TAGs. However, the appropriate TAGs, accumulated from microalgae biomass, can be used as substrates for different kinds of renewable liquid fuels such as biodiesel and jet fuel.
  
  
  RESULTS
  This article describes the isolation and identification of a lipid-rich, hydrocarbon-producing alga, Stichococcus bacillaris strain siva2011, together with its bioprocessing, hydrocarbon and fatty acid methyl ester (FAME) profiles. The S. bacillaris strain siva2011 was scaled-up in an 8 L bioreactor with 0.2% CO2. The C16:0, C16:3, C18:1, C18:2 and C18:3 were 112.2, 9.4, 51.3, 74.1 and 69.2 mg/g dry weight (DW), respectively. This new strain produced a significant amount of biomass of 3.79 g/L DW on day 6 in the 8 L bioreactor and also produced three hydrocarbons.
  
  
  CONCLUSIONS
  A new oil-rich microalga S. bacillaris strain siva2011 was discovered and its biomass has been scaled-up in a newly designed balloon-type bioreactor. The TAGs and hydrocarbons produced by this organism could be used as substrates for jet fuel or biodiesel.
authors.affiliation: Arkansas Biosciences Institute and College of Agriculture and Technology, Arkansas State University, PO Box 639, Jonesboro, AR 72401, USA.
authors.forename: Ganapathy
authors.initials: G
authors.lastname: Sivakumar
authors.affiliation: College of Engineering, Arkansas State University, Jonesboro, AR 72401, USA.
authors.forename: Kwangkook
authors.initials: K
authors.lastname: Jeong
authors.affiliation: Arkansas Statewide Mass Spectrometry Facility, University of Arkansas, Fayetteville, AR 72701, USA.
authors.forename: Jackson O
authors.initials: JO
authors.lastname: Lay
keywords: Algae; Bioreactor; Hydrocarbon; Jet fuel; Triacylglycerol
references.citation: Bioresour Technol. 2012 Aug;118:150-7
references.pmid: 22705518
references.citation: Plant Sci. 2012 Apr;185-186:9-22
references.pmid: 22325862
references.citation: Plant Physiol. 1985 Dec;79(4):1125-6
references.pmid: 16664542
references.citation: Bioresour Technol. 2013 Oct;146:569-573
references.pmid: 23973977
references.citation: Bioresour Technol. 2011 Apr;102(8):5076-82
references.pmid: 21354787
references.citation: Bioresour Technol. 2011 Apr;102(8):5083-92
references.pmid: 21324679
references.citation: Extremophiles. 2012 Jan;16(1):127-33
references.pmid: 22083129
references.citation: Biotechnol Bioeng. 2010 Dec 1;107(5):802-13
references.pmid: 20687140
references.citation: Biotechnol J. 2006 Dec;1(12):1419-27
references.pmid: 17136730
references.citation: Bioresour Technol. 2012 Mar;107:1-9
references.pmid: 22230775
references.citation: Proc Natl Acad Sci U S A. 2011 Jul 26;108(30):12260-5
references.pmid: 21746901
references.citation: Biochemistry. 2012 Oct 9;51(40):7908-16
references.pmid: 22947199
references.citation: Biotechnol J. 2011 Jan;6(1):66-73
references.pmid: 21086455
references.citation: Curr Med Chem. 2011;18(1):79-90
references.pmid: 21110813
references.citation: Biotechnol Biofuels. 2013 Feb 13;6(1):21
references.pmid: 23402697
references.citation: Protist. 2011 Apr;162(2):315-31
references.pmid: 21333593
references.citation: J Chromatogr A. 1999 Nov 12;862(2):221-9
references.pmid: 10596980
references.citation: Biotechnol Biofuels. 2013 May 06;6(1):69
references.pmid: 23641684
references.citation: Environ Sci Technol. 1994 Sep 1;28(9):1577-81
references.pmid: 22176357