title: On the limited recognition of inorganic surfaces by short peptides compared with antibodies.
abstract: The vast potential applications of biomolecules that bind inorganic surfaces led mostly to the isolation of short peptides that target selectively specific materials. The demonstrated differential affinity toward certain surfaces created the impression that the recognition capacity of short peptides may match that of rigid biomolecules. In the following, we challenge this view by comparing the capacity of antibody molecules to discriminate between the (100) and (111A) facets of a gallium arsenide semiconductor crystal with the capacity of short peptides to do the same. Applying selection from several peptide and single chain phage display libraries, we find a number of antibody molecules that bind preferentially a given crystal facet but fail to isolate, in dozens of attempts, a single peptide capable of such recognition. The experiments underscore the importance of rigidity to the recognition of inorganic flat targets and therefore set limitations on potential applications of short peptides in biomimetics.
authors.affiliation: Department of Biology, Technion - Israel Institute of Technology, Haifa, 32000, Israel; Department of Physics, Technion - Israel Institute of Technology, Haifa, 32000, Israel; The Russell Berrie Nanotechnology Institute, Technion - Israel Institute of Technology, Haifa, 32000, Israel.
authors.forename: Arbel
authors.initials: A
authors.lastname: Artzy-Schnirman
authors.forename: Enas
authors.initials: E
authors.lastname: Abu-Shah
authors.forename: Matan
authors.initials: M
authors.lastname: Dishon
authors.forename: Hadas
authors.initials: H
authors.lastname: Soifer
authors.forename: Yotam
authors.initials: Y
authors.lastname: Sivan
authors.forename: Yoram
authors.initials: Y
authors.lastname: Reiter
authors.forename: Itai
authors.initials: I
authors.lastname: Benhar
authors.forename: Uri
authors.initials: U
authors.lastname: Sivan
mesh_terms: D000906:Antibodies / Q000737:chemistry* / Q000276:immunology; D001152:Arsenicals / Q000737:chemistry* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005708:Gallium / Q000737:chemistry* / Q000276:immunology; D009842:Oligopeptides / Q000737:chemistry*; D012666:Semiconductors; D013499:Surface Properties
chemical_list: D000906:Antibodies; D001152:Arsenicals; D009842:Oligopeptides; C043055:gallium arsenide; D005708:Gallium
keywords: antibody binding to inorganic surface; biomimic; molecular recognition; peptide binding to inorganic surface; phage display