title: Microanalysis of oligosaccharide HS203 in beagle dog plasma by postcolumn fluorescence derivatization method.
abstract: A rapid and sensitive postcolumn fluorescence derivatization method was developed for microanalysis of antidiabetic oligosaccharide HS203 in beagle dog plasma. After plasma protein was removed by a simple and fast ultrafiltration method, chromatographic separation was performed on an Asahipak GS-320 HQ column with a mobile phase of 50 mmol/L phosphate buffer (pH 6.7) and acetonitrile (83/17, v/v). The column effluent was monitored by fluorescence detection at 249 nm (excitation) and 435 nm (emission) using guanidine hydrochloride as a postcolumn derivatizing reagent. A satisfactory resolution of the analyte was achieved and the limit of detection was found to be 4 ng (more sensitive than silver staining of HS203 in polyacrylamide gel electrophoresis). The method described above was successfully applied to a pharmacokinetic study of HS203 and to monitor blood glucose level simultaneously in beagle dog. It is also possible to be applied for microanalysis of other oligosaccharides in biological samples.
authors.affiliation: Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, 5 Yushan Road, Qingdao, Shandong 266003, China; Shandong Provincial Key Laboratory of Glycoscience and Glycoengineering, Ocean University of China, 5 Yushan Road, Qingdao, Shandong 266003, China.
authors.forename: Shumeng
authors.initials: S
authors.lastname: Sun
authors.affiliation: Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, 5 Yushan Road, Qingdao, Shandong 266003, China; Shandong Provincial Key Laboratory of Glycoscience and Glycoengineering, Ocean University of China, 5 Yushan Road, Qingdao, Shandong 266003, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Shandong Provincial Key Laboratory of Glycoscience and Glycoengineering, Ocean University of China, 5 Yushan Road, Qingdao, Shandong 266003, China.
authors.forename: Guangsheng
authors.initials: G
authors.lastname: Li
authors.affiliation: Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, 5 Yushan Road, Qingdao, Shandong 266003, China; Shandong Provincial Key Laboratory of Glycoscience and Glycoengineering, Ocean University of China, 5 Yushan Road, Qingdao, Shandong 266003, China.
authors.forename: Guangli
authors.initials: G
authors.lastname: Yu
authors.affiliation: Shandong Provincial Key Laboratory of Glycoscience and Glycoengineering, Ocean University of China, 5 Yushan Road, Qingdao, Shandong 266003, China.
authors.forename: Xiaoxu
authors.initials: X
authors.lastname: Xing
authors.affiliation: Shandong Provincial Key Laboratory of Glycoscience and Glycoengineering, Ocean University of China, 5 Yushan Road, Qingdao, Shandong 266003, China.
authors.forename: Yangyang
authors.initials: Y
authors.lastname: Zeng
authors.affiliation: Shandong Provincial Key Laboratory of Glycoscience and Glycoengineering, Ocean University of China, 5 Yushan Road, Qingdao, Shandong 266003, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Wu
authors.affiliation: Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, 5 Yushan Road, Qingdao, Shandong 266003, China.
authors.forename: Jianing
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel; D005453:Fluorescence; D007004:Hypoglycemic Agents / Q000097:blood* / Q000493:pharmacokinetics / Q000494:pharmacology; D009844:Oligosaccharides / Q000097:blood* / Q000493:pharmacokinetics / Q000494:pharmacology
chemical_list: D001786:Blood Glucose; D007004:Hypoglycemic Agents; D009844:Oligosaccharides