title: In Aspergillus nidulans the suppressors suaA and suaC code for release factors eRF1 and eRF3 and suaD codes for a glutamine tRNA.
abstract: In Aspergillus nidulans, after extensive mutagenesis, a collection of mutants was obtained and four suppressor loci were identified genetically that could suppress mutations in putative chain termination mutations in different genes. Suppressor mutations in suaB and suaD have a similar restricted spectrum of suppression and suaB111 was previously shown to be an alteration in the anticodon of a gln tRNA. We have shown that like suaB, a suaD suppressor has a mutation in the anticodon of another gln tRNA allowing suppression of UAG mutations. Mutations in suaA and suaC had a broad spectrum of suppression. Four suaA mutations result in alterations in the coding region of the eukaryotic release factor, eRF1, and another suaA mutation has a mutation in the upstream region of eRF1 that prevents splicing of the first intron within the 5'UTR. Epitope tagging of eRF1 in this mutant results in 20% of the level of eRF1 compared to the wild-type. Two mutations in suaC result in alterations in the eukaryotic release factor, eRF3. This is the first description in Aspergillus nidulans of an alteration in eRF3 leading to suppression of chain termination mutations.
authors.affiliation: Department of Biological, Biomedical and Environmental Sciences, University of Hull, Hull HU6 7RX, United Kingdom.
authors.forename: Wen
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Cellular and Molecular Biology, Centro de Investigaciones Biológicas (CSIC), Ramiro de Maeztu, 9, 28040 Madrid, Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: Mellado
authors.affiliation: Department of Cellular and Molecular Biology, Centro de Investigaciones Biológicas (CSIC), Ramiro de Maeztu, 9, 28040 Madrid, Spain.
authors.forename: Eduardo A
authors.initials: EA
authors.lastname: Espeso
authors.affiliation: Department of Biological, Biomedical and Environmental Sciences, University of Hull, Hull HU6 7RX, United Kingdom h.m.sealy-lewis@hull.ac.uk.
authors.forename: Heather M
authors.initials: HM
authors.lastname: Sealy-Lewis
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D001233:Aspergillus nidulans / Q000235:genetics* / Q000254:growth & development; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D018390:Gene Targeting; D016153:Genes, Suppressor; D056426:Genetic Loci; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D011487:Protein Conformation; D021381:Protein Transport; D012352:RNA, Transfer, Glu / Q000235:genetics*; D016415:Sequence Alignment
chemical_list: D005656:Fungal Proteins; D012352:RNA, Transfer, Glu
keywords: suaA codes for eRF1; suaC codes for eRF3; suaD codes for a glutamine tRNA; suppression of nonsense mutations in A. nidulans
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