title: GPC1 regulated by miR-96-5p, rather than miR-182-5p, in inhibition of pancreatic carcinoma cell proliferation.
abstract: To determine the relationships between miR-96-5p/-182-5p and GPC1 in pancreatic cancer (PC), we conducted the population and in vitro studies. We followed 38 pancreatic cancer patients, measured and compared the expression of miR-96-5p/-182-5p, GPC1, characteristics and patients' survival time of different miR-96-5p/-182-5p expression levels in PC tissues. In an in vitro study, we investigated the proliferation, cycle and apotosis in cells transfected with mimics/inhibitors of the two miRNAs, and determine their effects on GPC1 by dual-luciferase assay. In the follow-up study, we found that the expressions of miR-96-5p/-182-5p were lower/higher in PC tissues; patients with lower/higher levels of miR-96-5p/-182-5p suffered poorer characteristics and decreased survival time. In the in vitro study, the expressions of miR-96-5p/-182-5p were different in cells. Proliferation of cells transfected with miR-96-5p mimics/inhibitors was lower/higher in Panc-1/BxPC-3; when transfected with miR-182-5p mimics/inhibitors, proliferation of cells were higher/lower in AsPC-1/Panc-1. In a cell cycle study, panc-1 cells transfected with miR-96-5p mimics was arrested at G0/G1; BxPC-3 cells transfected with miR-96-5p inhibitors showed a significantly decrease at G0/G1; AsPC-1 cells transfected with miR-182-5p mimics was arrested at S; Panc-1 cells transfected with miR-182-5p inhibitors showed a decrease at S. MiR-96-5p mimics increased the apoptosis rate in Panc-1 cells, and its inhibitors decreased the apoptosis rate in BxPC-3. Dual luciferase assay revealed that GPC1 was regulated by miR-96-5p, not -182-5p. We found that miR-96-5p/-182-5p as good markers for PC; miR-96-5p, rather than -182-5p, inhibits GPC1 to suppress proliferation of PC cells.
authors.affiliation: Department of Hepatopancreatobiliary Surgery, Second Affiliated Hospital of Harbin Medical University, Harbin 150081, China. chunlong81@163.com.
authors.forename: Chunlong
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Laboratory Diagnostics, Fourth Affiliated Hospital of Harbin Medical University, Harbin 150001, China. duxuefei82@163.com.
authors.forename: Xuefei
authors.initials: X
authors.lastname: Du
authors.affiliation: Department of Hepatopancreatobiliary Surgery, Second Affiliated Hospital of Harbin Medical University, Harbin 150081, China. shengtai75@163.com.
authors.forename: Sheng
authors.initials: S
authors.lastname: Tai
authors.affiliation: Department of Hepatopancreatobiliary Surgery, Second Affiliated Hospital of Harbin Medical University, Harbin 150081, China. xiangyuzhong76@126.com.
authors.forename: Xiangyu
authors.initials: X
authors.lastname: Zhong
authors.affiliation: Department of Hepatopancreatobiliary Surgery, Second Affiliated Hospital of Harbin Medical University, Harbin 150081, China. zhidongwang74@126.com.
authors.forename: Zhidong
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Hepatopancreatobiliary Surgery, Second Affiliated Hospital of Harbin Medical University, Harbin 150081, China. zhanlianghu65@163.com.
authors.forename: Zhanliang
authors.initials: Z
authors.lastname: Hu
authors.affiliation: Department of Hepatopancreatobiliary Surgery, Second Affiliated Hospital of Harbin Medical University, Harbin 150081, China. leizhang74@163.com.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Hepatopancreatobiliary Surgery, Second Affiliated Hospital of Harbin Medical University, Harbin 150081, China. pengchengkang84@163.com.
authors.forename: Pengcheng
authors.initials: P
authors.lastname: Kang
authors.affiliation: Department of Hepatopancreatobiliary Surgery, Second Affiliated Hospital of Harbin Medical University, Harbin 150081, China. daolinji84@163.com.
authors.forename: Daolin
authors.initials: D
authors.lastname: Ji
authors.affiliation: Department of Hepatopancreatobiliary Surgery, Second Affiliated Hospital of Harbin Medical University, Harbin 150081, China. xingmingjiang87@163.com.
authors.forename: Xingming
authors.initials: X
authors.lastname: Jiang
authors.affiliation: Department of Hepatopancreatobiliary Surgery, Second Affiliated Hospital of Harbin Medical University, Harbin 150081, China. qingxinzhou88@yeah.net.
authors.forename: Qingxin
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: Department of Hepatopancreatobiliary Surgery, Second Affiliated Hospital of Harbin Medical University, Harbin 150081, China. mingwan84@163.com.
authors.forename: Ming
authors.initials: M
authors.lastname: Wan
authors.affiliation: Department of Hepatopancreatobiliary Surgery, Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou 310009, China. guixingjiang84@163.com.
authors.forename: Guixing
authors.initials: G
authors.lastname: Jiang
authors.affiliation: Department of Hepatopancreatobiliary Surgery, Second Affiliated Hospital of Harbin Medical University, Harbin 150081, China. yfcui356@163.com.
authors.forename: Yunfu
authors.initials: Y
authors.lastname: Cui
mesh_terms: D020121:5' Untranslated Regions; D017209:Apoptosis; D001483:Base Sequence; D002277:Carcinoma / Q000378:metabolism / Q000401:mortality / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D005260:Female; D059585:G1 Phase Cell Cycle Checkpoints; D005786:Gene Expression Regulation / Q000235:genetics*; D053673:Glypicans / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D008875:Middle Aged; D010190:Pancreatic Neoplasms / Q000378:metabolism / Q000401:mortality / Q000473:pathology; D015996:Survival Rate
chemical_list: D020121:5' Untranslated Regions; D053673:Glypicans; C541492:MIRN96 microRNA, human; D035683:MicroRNAs; C538984:Mirn182 microRNA, human
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