title: PRP19 upregulation inhibits cell proliferation in lung adenocarcinomas by p21-mediated induction of cell cycle arrest.
abstract: Precursor messenger RNA processing factor 19 (PRP19) is known to be a critical component of the eukaryotic spliceosomal machinery and DNA damage repair system, the deregulation of which leads to many disease conditions. In many human cancers, PRP19 expression is upregulated, but its functional significance and corresponding underlying mechanisms remain to be addressed. Focusing on lung carcinomas, PRP19 upregulation was achieved by plasmid transfection into A549 adenocarcinoma cells. The transfected cells were then subjected to several in vitro and in vivo assays following in situ assessment of the protein in paired clinical lung tissues. We report that PRP19 expression is elevated in lung carcinoma tissues compared to non-tumor tissues. Following its upregulation, PRP19 repressed cell proliferation and tumor growth by upregulating the expression of the cell cycle arrest protein p21.
authors.affiliation: Department of Histology and Embryology, Dalian Medical University, 9 Western Section, Lvshun South Road, Lvshunkou District, Dalian 116044, PR China; Liaoning Provincial Key Laboratory for Proteomics, Dalian Medical University, 9 Western Section, Lvshun South Road, Dalian 116044, PR China; School of Allied Health Sciences, University of Ghana, P. O. Box KB143, Accra, Ghana.
authors.forename: Arko-Boham
authors.initials: AB
authors.lastname: Benjamin
authors.affiliation: Department of Histology and Embryology, Dalian Medical University, 9 Western Section, Lvshun South Road, Lvshunkou District, Dalian 116044, PR China; Liaoning Provincial Key Laboratory for Proteomics, Dalian Medical University, 9 Western Section, Lvshun South Road, Dalian 116044, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Department of Histology and Embryology, Dalian Medical University, 9 Western Section, Lvshun South Road, Lvshunkou District, Dalian 116044, PR China; Liaoning Provincial Key Laboratory for Proteomics, Dalian Medical University, 9 Western Section, Lvshun South Road, Dalian 116044, PR China; Department of Anatomy, SMS, Kwame Nkrumah University of Science and Technology, PMB, Kumasi, Ghana.
authors.forename: Okai
authors.initials: O
authors.lastname: Isaac
authors.affiliation: Department of Histology and Embryology, Dalian Medical University, 9 Western Section, Lvshun South Road, Lvshunkou District, Dalian 116044, PR China; Liaoning Provincial Key Laboratory for Proteomics, Dalian Medical University, 9 Western Section, Lvshun South Road, Dalian 116044, PR China.
authors.forename: Haoqi
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Department of Histology and Embryology, Dalian Medical University, 9 Western Section, Lvshun South Road, Lvshunkou District, Dalian 116044, PR China; Liaoning Provincial Key Laboratory for Proteomics, Dalian Medical University, 9 Western Section, Lvshun South Road, Dalian 116044, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Song
authors.affiliation: Department of Histology and Embryology, Dalian Medical University, 9 Western Section, Lvshun South Road, Lvshunkou District, Dalian 116044, PR China; Liaoning Provincial Key Laboratory for Proteomics, Dalian Medical University, 9 Western Section, Lvshun South Road, Dalian 116044, PR China.
authors.forename: Xinming
authors.initials: X
authors.lastname: Chi
authors.affiliation: Department of Chest Surgery, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning, PR China.
authors.forename: Bing
authors.initials: B
authors.lastname: Sun
authors.affiliation: Department of Histology and Embryology, Dalian Medical University, 9 Western Section, Lvshun South Road, Lvshunkou District, Dalian 116044, PR China; Liaoning Provincial Key Laboratory for Proteomics, Dalian Medical University, 9 Western Section, Lvshun South Road, Dalian 116044, PR China.
authors.forename: Lihong
authors.initials: L
authors.lastname: Hao
authors.affiliation: Liaoning Provincial Key Laboratory for Proteomics, Dalian Medical University, 9 Western Section, Lvshun South Road, Dalian 116044, PR China.
authors.forename: Liyuan
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Liaoning Provincial Key Laboratory for Proteomics, Dalian Medical University, 9 Western Section, Lvshun South Road, Dalian 116044, PR China.
authors.forename: Lu
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Pathology, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning, PR China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Guan
authors.affiliation: Department of Histology and Embryology, Dalian Medical University, 9 Western Section, Lvshun South Road, Lvshunkou District, Dalian 116044, PR China; Liaoning Provincial Key Laboratory for Proteomics, Dalian Medical University, 9 Western Section, Lvshun South Road, Dalian 116044, PR China. Electronic address: shaoshujuan2006@126.com.
authors.forename: Shujuan
authors.initials: S
authors.lastname: Shao
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D000077192:Adenocarcinoma of Lung; D000818:Animals; D059447:Cell Cycle Checkpoints / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000235:genetics*; D045643:DNA Repair Enzymes / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009687:Nuclear Proteins / Q000235:genetics*; D000072260:RNA Splicing Factors; D014162:Transfection; D015854:Up-Regulation
chemical_list: D050759:Cyclin-Dependent Kinase Inhibitor p21; D009687:Nuclear Proteins; D000072260:RNA Splicing Factors; D045643:DNA Repair Enzymes; C417041:PRPF19 protein, human
keywords: Cell proliferation; Lung carcinoma; PRP19; p21