title: MALDI imaging analysis of neuropeptides in the Africanized honeybee (Apis mellifera) brain: effect of ontogeny.
abstract: The occurrence and spatial distribution of the neuropeptides AmTRP-5 and AST-1 in the honeybee brain were monitored via MALDI spectral imaging according to the ontogeny of Africanized Apis mellifera. The levels of these peptides increased in the brains of 0-15 day old honeybees, and this increase was accompanied by an increase in the number of in-hive activities performed by the nurse bees, followed by a decrease in the period from 15 to 25 days of age, in which the workers began to perform activities outside the nest (guarding and foraging). The results obtained in the present investigation suggest that AmTRP-5 acts in the upper region of both pedunculi of young workers, possibly regulating the cell cleaning and brood capping activities. Meanwhile, the localized occurrence of AmTRP-5 and AST-1 in the antennal lobes, subesophageal ganglion, upper region of the medulla, both lobula, and α- and β-lobes of both brain hemispheres in 20 to 25 day old workers suggest that the action of both neuropeptides in these regions may be related to their localized actions in these regions, regulating foraging and guarding activities. Thus, these neuropeptides appear to have some functions in the honeybee brain that are specifically related to the age-related division of labor.
authors.affiliation: Institute of Biosciences, Department of Biology, Center of the Study of Social Insects, University of São Paulo State (UNESP) , Avenue 24A 1515, Bela Vista, Rio Claro, 13506-900 SP, Brazil.
authors.forename: Marcel
authors.initials: M
authors.lastname: Pratavieira
authors.forename: Anally Ribeiro
authors.initials: AR
authors.lastname: da Silva Menegasso
authors.forename: Ana Maria Caviquioli
authors.initials: AM
authors.lastname: Garcia
authors.forename: Diego Simões
authors.initials: DS
authors.lastname: Dos Santos
authors.forename: Paulo Cesar
authors.initials: PC
authors.lastname: Gomes
authors.forename: Osmar
authors.initials: O
authors.lastname: Malaspina
authors.forename: Mario Sergio
authors.initials: MS
authors.lastname: Palma
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001516:Bees / Q000378:metabolism*; D001522:Behavior, Animal; D001921:Brain / Q000378:metabolism; D019476:Insect Proteins / Q000378:metabolism*; D059906:Neuroimaging; D009479:Neuropeptides / Q000378:metabolism*; D009928:Organ Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D019476:Insect Proteins; D009479:Neuropeptides