title: Vitamin D supplementation enhances the fixation of titanium implants in chronic kidney disease mice.
abstract: Vitamin D (Vit D) deficiency is a common condition in chronic kidney disease (CKD) patients that negatively affects bone regeneration and fracture healing. Previous study has shown that timely healing of titanium implants is impaired in CKD. This study aimed to investigate the effect of Vit D supplementation on implant osseointegration in CKD mice. Uremia was induced by 5/6 nephrectomy in C57BL mice. Eight weeks after the second renal surgery, animals were given 1,25(OH)2D3 three times a week intraperitoneally for four weeks. Experimental titanium implants were inserted into the distal end of femurs two weeks later. Serum measurements confirmed decreased 1,25(OH)2D levels in CKD mice, which could be successfully corrected by Vit D injections. Moreover, the hyperparathyroidism observed in CKD mice was also corrected. X-ray examination and histological sections showed successful osseointegration in these mice. Histomorphometrical analysis revealed that the bone-implant contact (BIC) ratio and bone volume (BV/TV) around the implant were significantly increased in the Vit D-supplementation group. In addition, resistance of the implant, as measured by a push-in method, was significantly improved compared to that in the vehicle group. These results demonstrate that Vit D supplementation is an effective approach to improve the fixation of titanium implants in CKD.
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
authors.forename: Weiqing
authors.initials: W
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
authors.forename: Shiwen
authors.initials: S
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
authors.forename: Huawei
authors.initials: H
authors.lastname: Zou
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
authors.forename: Ningyuan
authors.initials: N
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Liang
authors.affiliation: Department of Periodontology and Implant Dentistry, New York University College of Dentistry, New York, United States of America.
authors.forename: Michel
authors.initials: M
authors.lastname: Dard
authors.affiliation: Department of Oral Medicine, Infection, and Immunity, Harvard School of Dental medicine, Boston, Massachusetts, United States of America.
authors.forename: Beate
authors.initials: B
authors.lastname: Lanske
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
authors.forename: Quan
authors.initials: Q
authors.lastname: Yuan
mesh_terms: D000818:Animals; D019587:Dietary Supplements; D005260:Female; D005269:Femur / Q000000981:diagnostic imaging / Q000187:drug effects; D020007:Implants, Experimental; D008422:Materials Testing; D008810:Mice, Inbred C57BL; D009984:Orthopedic Fixation Devices; D011859:Radiography; D051436:Renal Insufficiency, Chronic / Q000097:blood / Q000000981:diagnostic imaging / Q000628:therapy*; D014025:Titanium / Q000494:pharmacology*; D014807:Vitamin D / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects
chemical_list: D014807:Vitamin D; D014025:Titanium
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