title: The effect of lysophosphatidic acid during in vitro maturation of bovine oocytes: embryonic development and mRNA abundances of genes involved in apoptosis and oocyte competence.
abstract: In the present study we examined whether LPA can be synthesized and act during in vitro maturation of bovine cumulus oocyte complexes (COCs). We found transcription of genes coding for enzymes of LPA synthesis pathway (ATX and PLA2) and of LPA receptors (LPAR 1-4) in bovine oocytes and cumulus cells, following in vitro maturation. COCs were matured in vitro in presence or absence of LPA (10(-5) M) for 24 h. Supplementation of maturation medium with LPA increased mRNA abundance of FST and GDF9 in oocytes and decreased mRNA abundance of CTSs in cumulus cells. Additionally, oocytes stimulated with LPA had higher transcription levels of BCL2 and lower transcription levels of BAX resulting in the significantly lower BAX/BCL2 ratio. Blastocyst rates on day 7 were similar in the control and the LPA-stimulated COCs. Our study demonstrates for the first time that bovine COCs are a potential source and target of LPA action. We postulate that LPA exerts an autocrine and/or paracrine signaling, through several LPARs, between the oocyte and cumulus cells. LPA supplementation of maturation medium improves COC quality, and although this was not translated into an enhanced in vitro development until the blastocyst stage, improved oocyte competence may be relevant for subsequent in vivo survival.
authors.affiliation: Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-747 Olsztyn, Poland.
authors.forename: Dorota
authors.initials: D
authors.lastname: Boruszewska
authors.affiliation: CIISA, Faculty of Veterinary Medicine, University of Lisbon, 1300-477 Lisbon, Portugal.
authors.forename: Ana Catarina
authors.initials: AC
authors.lastname: Torres
authors.affiliation: Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-747 Olsztyn, Poland.
authors.forename: Ilona
authors.initials: I
authors.lastname: Kowalczyk-Zieba
authors.affiliation: CIISA, Faculty of Veterinary Medicine, University of Lisbon, 1300-477 Lisbon, Portugal.
authors.forename: Patricia
authors.identifier: 0000-0001-5303-6953
authors.initials: P
authors.lastname: Diniz
authors.affiliation: CIISA, Faculty of Veterinary Medicine, University of Lisbon, 1300-477 Lisbon, Portugal.
authors.forename: Mariana
authors.initials: M
authors.lastname: Batista
authors.affiliation: CIISA, Faculty of Veterinary Medicine, University of Lisbon, 1300-477 Lisbon, Portugal.
authors.forename: Luis
authors.initials: L
authors.lastname: Lopes-da-Costa
authors.affiliation: Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-747 Olsztyn, Poland.
authors.forename: Izabela
authors.initials: I
authors.lastname: Woclawek-Potocka
mesh_terms: D000818:Animals; D017209:Apoptosis; D001755:Blastocyst / Q000378:metabolism; D002417:Cattle; D002478:Cells, Cultured; D054885:Cumulus Cells / Q000166:cytology; D005260:Female; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D054501:Group II Phospholipases A2 / Q000378:metabolism; D008246:Lysophospholipids / Q000737:chemistry*; D009865:Oocytes / Q000166:cytology*; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D013997:Time Factors; D051028:bcl-2-Associated X Protein / Q000378:metabolism; D000099189:Lysophospholipase D
chemical_list: D054501:Group II Phospholipases A2; D008246:Lysophospholipids; D010727:Phosphoric Diester Hydrolases; D012333:RNA, Messenger; D051028:bcl-2-Associated X Protein; D000099189:Lysophospholipase D; C032881:lysophosphatidic acid
references.citation: Biol Reprod. 1980 Apr;22(3):669-73
references.pmid: 7388112
references.citation: Biol Reprod. 2000 Sep;63(3):839-45
references.pmid: 10952929
references.citation: Dev Biol. 2001 Jan 1;229(1):224-36
references.pmid: 11133166
references.citation: Biochimie. 2010 Jun;92(6):698-706
references.pmid: 20417246
references.citation: Biol Reprod. 2004 Oct;71(4):1359-66
references.pmid: 15189828
references.citation: Mol Reprod Dev. 2000 Aug;56(4):483-94
references.pmid: 10911398
references.citation: Dev Biol. 2003 Dec 1;264(1):50-63
references.pmid: 14623231
references.citation: Biochim Biophys Acta. 2008 Sep;1781(9):513-8
references.pmid: 18621144
references.citation: Anim Reprod Sci. 2009 Aug;114(1-3):89-98
references.pmid: 19008057
references.citation: Trends Endocrinol Metab. 2010 Jan;21(1):17-24
references.pmid: 19836970
references.citation: Biol Reprod. 2002 Dec;67(6):1777-89
references.pmid: 12444053
references.citation: Dev Biol. 1996 Mar 15;174(2):335-44
references.pmid: 8631505
references.citation: Theriogenology. 2005 May;63(8):2147-63
references.pmid: 15826680
references.citation: Dev Biol. 1998 Dec 15;204(2):373-84
references.pmid: 9882477
references.citation: Biol Reprod. 2008 Aug;79(2):328-36
references.pmid: 18448840
references.citation: Oncogene. 2004 Jan 8;23(1):122-9
references.pmid: 14712217
references.citation: Life Sci. 2006 Jun 27;79(5):506-11
references.pmid: 16492384
references.citation: Life Sci. 2002 Jan 4;70(7):759-67
references.pmid: 11833739
references.citation: Proc Natl Acad Sci U S A. 1999 Apr 27;96(9):5233-8
references.pmid: 10220449
references.citation: Reprod Biol. 2013 Mar;13(1):82-7
references.pmid: 23522075
references.citation: Dev Biol. 1976 Jun;50(2):531-6
references.pmid: 1278599
references.citation: J Biol Chem. 2000 Dec 15;275(50):39523-8
references.pmid: 10998422
references.citation: J Biol Chem. 2003 Jul 11;278(28):25600-6
references.pmid: 12724320
references.citation: Reproduction. 2007 Jan;133(1):95-106
references.pmid: 17244736
references.citation: Reproduction. 2009 Jan;137(1):95-105
references.pmid: 18829944
references.citation: Reproduction. 2001 Sep;122(3):481-6
references.pmid: 11597313
references.citation: Oncogene. 2012 Apr 19;31(16):2075-89
references.pmid: 21996742
references.citation: Mol Endocrinol. 1999 Jun;13(6):1035-48
references.pmid: 10379900
references.citation: Cancer Res. 1999 Oct 15;59(20):5370-5
references.pmid: 10537322
references.citation: Dev Biol. 1991 Jan;143(1):162-72
references.pmid: 1985016
references.citation: J Biol Chem. 1984 Dec 25;259(24):15188-95
references.pmid: 6511790
references.citation: Experientia. 1996 Jul 15;52(7):647-51
references.pmid: 8698104
references.citation: Anim Reprod Sci. 2002 Apr 15;70(3-4):159-69
references.pmid: 11943486
references.citation: Endocrinology. 2009 May;150(5):2463-71
references.pmid: 19179440
references.citation: Mol Cell Endocrinol. 2013 Sep 5;377(1-2):103-11
references.pmid: 23856004
references.citation: Proc Natl Acad Sci U S A. 2003 Jan 7;100(1):131-6
references.pmid: 12502787
references.citation: J Biol Chem. 1999 Sep 24;274(39):27776-85
references.pmid: 10488122
references.citation: J Cell Sci. 2005 Nov 15;118(Pt 22):5257-68
references.pmid: 16263764
references.citation: Exp Cell Res. 2004 Jun 10;296(2):317-26
references.pmid: 15149861
references.citation: Semin Cell Dev Biol. 2004 Oct;15(5):477-89
references.pmid: 15271293
references.citation: FEBS Lett. 1994 Aug 29;351(1):38-40
references.pmid: 8076690
references.citation: Reprod Biol. 2013 Dec;13(4):344-7
references.pmid: 24287044
references.citation: Mol Reprod Dev. 2002 Mar;61(3):414-24
references.pmid: 11835587
references.citation: Nature. 2005 May 5;435(7038):104-8
references.pmid: 15875025
references.citation: Dev Biol. 1994 Jul;164(1):1-9
references.pmid: 8026614
references.citation: Biol Reprod. 2000 May;62(5):1209-17
references.pmid: 10775168
references.citation: Reprod Biol. 2013 Mar;13(1):100-3
references.pmid: 23522079
references.citation: Reproduction. 2004 Sep;128(3):313-9
references.pmid: 15333782
references.citation: In Vitro Cell Dev Biol Anim. 2009 Jul 8;:
references.pmid: 19585176
references.citation: Anim Reprod Sci. 2012 Oct;134(3-4):125-34
references.pmid: 22898494
references.citation: Theriogenology. 2002 Mar 1;57(4):1347-55
references.pmid: 12013454
references.citation: Mol Cell Biol. 2006 Jul;26(13):5015-22
references.pmid: 16782887
references.citation: Anat Embryol (Berl). 2000 Dec;202(6):523-9
references.pmid: 11131018
references.citation: Dev Biol. 2006 Aug 15;296(2):514-21
references.pmid: 16854407
references.citation: J Biol Chem. 2008 Mar 21;283(12):7470-9
references.pmid: 18198181
references.citation: Dev Biol. 1990 Mar;138(1):16-25
references.pmid: 2155145
references.citation: Anal Biochem. 2007 Aug 1;367(1):20-7
references.pmid: 17568554
references.citation: J Reprod Dev. 2010 Aug;56(4):411-20
references.pmid: 20431249
references.citation: Biol Reprod. 2003 May;68(5):1584-9
references.pmid: 12606495
references.citation: Biol Reprod. 1995 Dec;53(6):1243-50
references.pmid: 8562677
references.citation: In Vitro Cell Dev Biol Anim. 2013 Feb;49(2):134-46
references.pmid: 23358866
references.citation: Anim Reprod Sci. 2002 Oct 16;73(3-4):129-40
references.pmid: 12363437
references.citation: Dev Biol. 2000 Oct 15;226(2):167-79
references.pmid: 11023678
references.citation: Endocrinology. 2009 Jan;150(1):422-34
references.pmid: 18772233
references.citation: Hum Reprod. 2014 Feb;29(2):234-41
references.pmid: 24293550
references.citation: J Reprod Dev. 2012;58(6):661-71
references.pmid: 22972186
references.citation: Anim Reprod Sci. 2002 Jun 17;71(3-4):143-55
references.pmid: 12047924
references.citation: Exp Biol Med (Maywood). 2009 Aug;234(8):986-93
references.pmid: 19491366
references.citation: Theriogenology. 2004 Jun;61(9):1675-89
references.pmid: 15019463
references.citation: Biol Reprod. 2008 Aug;79(2):301-9
references.pmid: 18417713
references.citation: Mol Pharmacol. 2000 Apr;57(4):753-9
references.pmid: 10727522
references.citation: Theriogenology. 1999 Sep;52(4):683-700
references.pmid: 10734366
references.citation: Fertil Steril. 2005 Sep;84(3):627-33
references.pmid: 16169395
references.citation: Mol Reprod Dev. 1991 Mar;28(3):255-9
references.pmid: 2015084
references.citation: Reproduction. 2005 Oct;130(4):475-83
references.pmid: 16183865
references.citation: Biol Reprod. 1999 Jul;61(1):195-9
references.pmid: 10377049
references.citation: Biol Reprod. 1997 Dec;57(6):1420-5
references.pmid: 9408249
references.citation: Reproduction. 2010 Jul;140(1):73-82
references.pmid: 20395426
references.citation: Biol Reprod. 2000 Sep;63(3):805-10
references.pmid: 10952924