title: The Ph-3 gene from Solanum pimpinellifolium encodes CC-NBS-LRR protein conferring resistance to Phytophthora infestans.
abstract: Ph-3 is the first cloned tomato gene for resistance to late blight and encodes a CC-NBS-LRR protein. Late blight, caused by Phytophthora infestans, is one of the most destructive diseases in tomato. The resistance (R) gene Ph-3, derived from Solanum pimpinellifolium L3708, provides resistance to multiple P. infestans isolates and has been widely used in tomato breeding programmes. In our previous study, Ph-3 was mapped into a region harbouring R gene analogues (RGA) at the distal part of long arm of chromosome 9. To further narrow down the Ph-3 interval, more recombinants were identified using the flanking markers G2-4 and M8-2, which defined the Ph-3 gene to a 26 kb region according to the Heinz1706 reference genome. To clone the Ph-3 gene, a bacterial artificial chromosome (BAC) library was constructed using L3708 and one BAC clone B25E21 containing the Ph-3 region was identified. The sequence of the BAC clone B25E21 showed that only one RGA was present in the target region. A subsequent complementation analysis demonstrated that this RGA, encoding a CC-NBS-LRR protein, was able to complement the susceptible phenotype in cultivar Moneymaker. Thus this RGA was considered the Ph-3 gene. The predicted Ph-3 protein shares high amino acid identity with the chromosome-9-derived potato resistance proteins against P. infestans (Rpi proteins).
authors.affiliation: The Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Zhongguancunnandajie 12, 100081, Beijing, People's Republic of China.
authors.forename: Chunzhi
authors.initials: C
authors.lastname: Zhang
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.forename: Xiaoxuan
authors.initials: X
authors.lastname: Wang
authors.forename: Jack
authors.initials: J
authors.lastname: Vossen
authors.forename: Guangcun
authors.initials: G
authors.lastname: Li
authors.forename: Tao
authors.initials: T
authors.lastname: Li
authors.forename: Zheng
authors.initials: Z
authors.lastname: Zheng
authors.forename: Jianchang
authors.initials: J
authors.lastname: Gao
authors.forename: Yanmei
authors.initials: Y
authors.lastname: Guo
authors.forename: Richard G F
authors.initials: RG
authors.lastname: Visser
authors.forename: Junming
authors.initials: J
authors.lastname: Li
authors.forename: Yuling
authors.initials: Y
authors.lastname: Bai
authors.forename: Yongchen
authors.initials: Y
authors.lastname: Du
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D060467:Disease Resistance / Q000235:genetics*; D017343:Genes, Plant; D008969:Molecular Sequence Data; D055750:Phytophthora infestans; D010935:Plant Diseases / Q000382:microbiology*; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci; D016415:Sequence Alignment; D032322:Solanum / Q000235:genetics*
chemical_list: D010940:Plant Proteins
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