title: Electrospinning of poly(lactic acid)/polyhedral oligomeric silsesquioxane nanocomposites and their potential in chondrogenic tissue regeneration.
abstract: The study was conducted to evaluate the cytocompatibility and hydrolytic degradability of the new poly(lactic acid)/polyethylene glycol-polyhedral oligomeric silsesquioxane (peg-POSS/PLLA) nanocomposite as potential material for cartilage regeneration. PLLA scaffolds containing 0 to 5% of peg-POSS were fabricated by electrospinning. Human mesenchymal stem cells (hMSC's) were cultured in vitro to evaluate the cytocompatibility of the new nanocomposite material. Hydrolytic degradation studies were also carried out to analyze the mass loss rate of the nanocomposites through time. The addition of the peg-POSS to the PLLA did not affect the processability of the nanocomposite by electrospinning. It was also observed that peg-POSS did not show any relevant change in fibers morphology, concluding that it was well dispersed. However, addition of peg-POSS caused noticeable decrease in mean fiber diameter, which made the specific surface area of the scaffold to rise. hMSC's were able to attach, to proliferate, and to differentiate into chondrocytes in a similar way onto the different types of electrospun peg-POSS/PLLA and pure PLLA scaffolds, showing that the peg-POSS as nano-additive does not exhibit any cytotoxicity. The hydrolytic degradation rate of the material was lower when peg-POSS was added, showing a higher durability of the nanocomposites through time. Results demonstrate that the addition of peg-POSS to the PLLA scaffolds does not affect its cytocompatibility to obtain hyaline cartilage from hMSC's.
authors.affiliation: a Mechanical and Industrial Production Department , Mondragon Unibertsitatea , Loramendi, 4, 20500 Arrasate-Mondragon , Spain.
authors.forename: C
authors.initials: C
authors.lastname: Gomez-Sanchez
authors.forename: T
authors.initials: T
authors.lastname: Kowalczyk
authors.forename: G
authors.initials: G
authors.lastname: Ruiz De Eguino
authors.forename: A
authors.initials: A
authors.lastname: Lopez-Arraiza
authors.forename: A
authors.initials: A
authors.lastname: Infante
authors.forename: C I
authors.initials: CI
authors.lastname: Rodriguez
authors.forename: T A
authors.initials: TA
authors.lastname: Kowalewski
authors.forename: M
authors.initials: M
authors.lastname: Sarrionandia
authors.forename: J
authors.initials: J
authors.lastname: Aurrekoetxea
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D019902:Chondrocytes / Q000166:cytology / Q000187:drug effects; D020219:Chondrogenesis / Q000187:drug effects*; D004560:Electricity; D006801:Humans; D006868:Hydrolysis; D019344:Lactic Acid / Q000737:chemistry*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects; D053761:Nanocomposites / Q000737:chemistry*; D017646:Organosilicon Compounds / Q000737:chemistry*; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D012038:Regeneration / Q000187:drug effects*; D054457:Tissue Scaffolds / Q000737:chemistry; D014783:Viscosity
chemical_list: D001672:Biocompatible Materials; D017646:Organosilicon Compounds; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide)
keywords: PLLA; cartilage repair; chondrocyte; electrospinning; hydrolytic degradation; nanocomposite; peg-POSS; scaffold; stem cells