title: Suppressive effects of coixol, glyceryl trilinoleate and natural products derived from Coix Lachryma-Jobi var. ma-yuen on gene expression, production and secretion of airway MUC5AC mucin.
abstract: In this study, we investigated whether natural products including coixol derived from Coix Lachryma-Jobi var. ma-yuen affect MUC5AC mucin gene expression, production and secretion from airway epithelial cells. Confluent NCI-H292 cells were pretreated with oleic acid, linoleic acid, glyceryl trilinoleate, beta-stigmasterol or coixol for 30 min and then stimulated with PMA (phorbol 12-myristate 13-acetate), EGF (epidermal growth factor) or TNF-α (tumor necrosis factor-α) for 24 h. The MUC5AC mucin gene expression, mucin protein production and secretion were measured by RT-PCR and ELISA. The results were as follows: (1) Oleic acid, linoleic acid, glyceryl trilinoleate, beta-stigmasterol and coixol inhibited the expression of MUC5AC mucin gene induced by PMA from NCI-H292 cells; (2) Oleic acid, linoleic acid, glyceryl trilinoleate, beta-stigmasterol and coixol also inhibited the production of MUC5AC mucin protein induced by the same inducers from NCI-H292 cells; (3) Coixol inhibited the expression of MUC5AC mucin gene and production of MUC5AC mucin protein, induced by EGF or TNF-α from NCI-H292 cells; (4) Coixol decreased PMA-induced MUC5AC mucin secretion from NCI-H292 cells. This result suggests that coixol, the characteristic component among the examined five natural products derived from C. Lachryma-Jobi var. ma-yuen, can regulate gene expression, production and secretion of mucin, by directly acting on airway epithelial cells.
authors.affiliation: Department of Pharmacology, School of Medicine, Chungnam National University, 6 Munhwa-Dong, Joong-Gu, Daejeon, 303-131, Korea.
authors.forename: Hyun Jae
authors.initials: HJ
authors.lastname: Lee
authors.forename: Jiho
authors.initials: J
authors.lastname: Ryu
authors.forename: Su Hyun
authors.initials: SH
authors.lastname: Park
authors.forename: Eun-Kyoung
authors.initials: EK
authors.lastname: Seo
authors.forename: Ah-Reum
authors.initials: AR
authors.lastname: Han
authors.forename: Sang Kook
authors.initials: SK
authors.lastname: Lee
authors.forename: Yeong Shik
authors.initials: YS
authors.lastname: Kim
authors.forename: Jang-Hee
authors.initials: JH
authors.lastname: Hong
authors.forename: Jeong Ho
authors.initials: JH
authors.lastname: Seok
authors.forename: Choong Jae
authors.initials: CJ
authors.lastname: Lee
mesh_terms: D001583:Benzoxazoles / Q000302:isolation & purification* / Q000494:pharmacology; D001688:Biological Products / Q000302:isolation & purification* / Q000494:pharmacology; D045744:Cell Line, Tumor; D031742:Coix; D005786:Gene Expression Regulation; D006801:Humans; D055271:Mucin 5AC / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000378:metabolism; D009077:Mucins / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000378:metabolism; D020545:Respiratory Mucosa / Q000187:drug effects / Q000378:metabolism*; D014280:Triglycerides / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: D001583:Benzoxazoles; D001688:Biological Products; C524678:MUC5AC protein, human; D055271:Mucin 5AC; D009077:Mucins; D014280:Triglycerides; C031896:6-methoxybenzoxazolinone; C009564:trilinolein