title: Re-evaluation of physicochemical and NMR data of triol ginsenosides Re, Rf, Rg2, and 20-gluco-Rf from Panax ginseng roots.
abstract: Ginseng roots were extracted with aqueous methanol, and extracts were suspended in water and extracted successively with ethyl acetate and n-butanol. Column chromatography using the n-butanol fraction yielded four purified triol ginseng saponins: the ginsenosides Re, Rf, Rg2, and 20-gluco-Rf. The physicochemical, spectroscopic, and chromatographic characteristics of the ginsenosides were measured and compared with reports from the literature. For spectroscopic analysis, two-dimensional nuclear magnetic resonance (NMR) methods such as (1)H-(1)H correlation spectroscopy, nuclear Overhauser effect spectroscopy, heteronuclear single quantum correlation, and heteronuclear multiple bond connectivity were employed to identify exact peak assignments. Some peak assignments for previously published (1)H- and (13)C-NMR spectra were found to be inaccurate. This study reports the complete NMR assignment of 20-gluco-Rf for the first time.
authors.affiliation: Graduate School of Biotechnology and Department of Oriental Medicinal Materials and Processing, Kyung Hee University, Yongin, Korea.
authors.forename: Jin-Gyeong
authors.initials: JG
authors.lastname: Cho
authors.affiliation: Graduate School of Biotechnology and Department of Oriental Medicinal Materials and Processing, Kyung Hee University, Yongin, Korea.
authors.forename: Seo-Ji
authors.initials: SJ
authors.lastname: In
authors.affiliation: Graduate School of Biotechnology and Department of Oriental Medicinal Materials and Processing, Kyung Hee University, Yongin, Korea.
authors.forename: Ye-Jin
authors.initials: YJ
authors.lastname: Jung
authors.affiliation: Graduate School of Biotechnology and Department of Oriental Medicinal Materials and Processing, Kyung Hee University, Yongin, Korea.
authors.forename: Byeong-Ju
authors.initials: BJ
authors.lastname: Cha
authors.affiliation: Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration, Eumseong, Korea.
authors.forename: Dae-Young
authors.initials: DY
authors.lastname: Lee
authors.affiliation: Technology Services Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Suwon, Korea.
authors.forename: Yong-Bum
authors.initials: YB
authors.lastname: Kim
authors.affiliation: Amorepacific Corporation Research and Development Center, Yongin, Korea.
authors.forename: Myeonghun
authors.initials: M
authors.lastname: Yeom
authors.affiliation: Graduate School of Biotechnology and Department of Oriental Medicinal Materials and Processing, Kyung Hee University, Yongin, Korea.
authors.forename: Nam-In
authors.initials: NI
authors.lastname: Baek
keywords: HPLC; NMR; Panax ginseng; fast atom bombardment/MS; triol ginsenoside
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