title: A new molecular surveillance system for leishmaniasis.
abstract: Presently, global efforts are being made to control and eradicate the deadliest tropical diseases through the improvement of adequate interventions. A critical point for programs to succeed is the prompt and accurate diagnosis in endemic regions. Rapid diagnostic tests (RDTs) are being massively deployed and used to improve diagnosis in tropical countries. In the present report, we evaluated the hypothesis of, after use for diagnosis, the reuse of the Leishmania RDT kit as a DNA source, which can be used downstream as a molecular surveillance and/or quality control tool. As a proof of principle, a polymerase chain reaction-based method was used to detect Leishmania spp. minicircle kinetoplast DNA from leishmaniasis RDT kits. Our results show that Leishmania spp. DNA can be extracted from used RDTs and may constitute an important, reliable, and affordable tool to assist in future leishmaniasis molecular surveillance methods.
authors.affiliation: Department of Protozoology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan; Sukraraj Tropical and Infectious Disease Hospital, Kathmandu, Nepal; Janakpur Zonal Hospital, Janakpur, Nepal; Department of Parasitology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan; Department of Microbiology, Tumor and Cell Biology, Karolinska Institute, Stockholm, Sweden; Nanyang Technological University, School of Biological Sciences, Singapore.
authors.forename: Kishor
authors.initials: K
authors.lastname: Pandey
authors.affiliation: Department of Protozoology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan; Sukraraj Tropical and Infectious Disease Hospital, Kathmandu, Nepal; Janakpur Zonal Hospital, Janakpur, Nepal; Department of Parasitology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan; Department of Microbiology, Tumor and Cell Biology, Karolinska Institute, Stockholm, Sweden; Nanyang Technological University, School of Biological Sciences, Singapore.
authors.forename: Basu Dev
authors.initials: BD
authors.lastname: Pandey
authors.affiliation: Department of Protozoology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan; Sukraraj Tropical and Infectious Disease Hospital, Kathmandu, Nepal; Janakpur Zonal Hospital, Janakpur, Nepal; Department of Parasitology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan; Department of Microbiology, Tumor and Cell Biology, Karolinska Institute, Stockholm, Sweden; Nanyang Technological University, School of Biological Sciences, Singapore.
authors.forename: Arun Kumar
authors.initials: AK
authors.lastname: Mallik
authors.affiliation: Department of Protozoology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan; Sukraraj Tropical and Infectious Disease Hospital, Kathmandu, Nepal; Janakpur Zonal Hospital, Janakpur, Nepal; Department of Parasitology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan; Department of Microbiology, Tumor and Cell Biology, Karolinska Institute, Stockholm, Sweden; Nanyang Technological University, School of Biological Sciences, Singapore.
authors.forename: Jyoti
authors.initials: J
authors.lastname: Acharya
authors.affiliation: Department of Protozoology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan; Sukraraj Tropical and Infectious Disease Hospital, Kathmandu, Nepal; Janakpur Zonal Hospital, Janakpur, Nepal; Department of Parasitology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan; Department of Microbiology, Tumor and Cell Biology, Karolinska Institute, Stockholm, Sweden; Nanyang Technological University, School of Biological Sciences, Singapore.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Kato
authors.affiliation: Department of Protozoology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan; Sukraraj Tropical and Infectious Disease Hospital, Kathmandu, Nepal; Janakpur Zonal Hospital, Janakpur, Nepal; Department of Parasitology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan; Department of Microbiology, Tumor and Cell Biology, Karolinska Institute, Stockholm, Sweden; Nanyang Technological University, School of Biological Sciences, Singapore.
authors.forename: Osamu
authors.initials: O
authors.lastname: Kaneko
authors.affiliation: Department of Protozoology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan; Sukraraj Tropical and Infectious Disease Hospital, Kathmandu, Nepal; Janakpur Zonal Hospital, Janakpur, Nepal; Department of Parasitology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan; Department of Microbiology, Tumor and Cell Biology, Karolinska Institute, Stockholm, Sweden; Nanyang Technological University, School of Biological Sciences, Singapore pedro.ferreira@ki.se.
authors.forename: Pedro Eduardo
authors.initials: PE
authors.lastname: Ferreira
mesh_terms: D000328:Adult; D000368:Aged; D018105:DNA, Kinetoplast / Q000097:blood; D016054:DNA, Protozoan / Q000097:blood; D019353:Endemic Diseases; D062665:Epidemiological Monitoring; D005260:Female; D006801:Humans; D007891:Leishmania / Q000235:genetics / Q000302:isolation & purification*; D007896:Leishmaniasis / Q000175:diagnosis / Q000453:epidemiology*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D013997:Time Factors
chemical_list: D018105:DNA, Kinetoplast; D016054:DNA, Protozoan; D011933:Reagent Kits, Diagnostic
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