title: Boosting accumulation of neutral lipids in Rhodosporidium kratochvilovae HIMPA1 grown on hemp (Cannabis sativa Linn) seed aqueous extract as feedstock for biodiesel production.
abstract: Hemp seeds aqueous extract (HSAE) was used as cheap renewable feedstocks to grow novel oleaginous yeast Rhodosporidium kratochvilovae HIMPA1 isolated from Himalayan permafrost soil. The yeast showed boosted triglyceride (TAG) accumulation in the lipid droplets (LDs) which were transesterified to biodiesel. The sonicated HSAE prepared lacked toxic inhibitors and showed enhanced total lipid content and lipid yield 55.56%, 8.39±0.57g/l in comparison to 41.92%, 6.2±0.8g/l from industrially used glucose synthetic medium, respectively. Supersized LDs (5.95±1.02μm) accumulated maximum TAG in sonicated HSAE grown cells were visualized by fluorescent BODIPY (505/515nm) stain. GC-MS analysis revealed unique longer carbon chain FAME profile containing Arachidic acid (C20:0) 5%, Behenic acid (C22:0) 9.7%, Heptacosanoic acid (C27:0) 14.98%, for the first time in this yeast when grown on industrially competent sonicated HSAE, showing more similarity to algal oils.
authors.affiliation: Molecular Microbiology Laboratory, Biotechnology Department, Indian Institute of Technology Roorkee (IIT R), Roorkee, Uttarakhand 247667, India.
authors.forename: Alok
authors.initials: A
authors.lastname: Patel
authors.affiliation: Molecular Microbiology Laboratory, Biotechnology Department, Indian Institute of Technology Roorkee (IIT R), Roorkee, Uttarakhand 247667, India.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Pravez
authors.affiliation: Molecular Microbiology Laboratory, Biotechnology Department, Indian Institute of Technology Roorkee (IIT R), Roorkee, Uttarakhand 247667, India.
authors.forename: Farha
authors.initials: F
authors.lastname: Deeba
authors.affiliation: Molecular Microbiology Laboratory, Biotechnology Department, Indian Institute of Technology Roorkee (IIT R), Roorkee, Uttarakhand 247667, India.
authors.forename: Vikas
authors.initials: V
authors.lastname: Pruthi
authors.affiliation: Molecular Microbiology Laboratory, Biotechnology Department, Indian Institute of Technology Roorkee (IIT R), Roorkee, Uttarakhand 247667, India.
authors.forename: Rajesh P
authors.initials: RP
authors.lastname: Singh
authors.affiliation: Molecular Microbiology Laboratory, Biotechnology Department, Indian Institute of Technology Roorkee (IIT R), Roorkee, Uttarakhand 247667, India. Electronic address: parulaggarwalpruthi@gmail.com.
authors.forename: Parul A
authors.initials: PA
authors.lastname: Pruthi
mesh_terms: D001487:Basidiomycota / Q000166:cytology / Q000187:drug effects / Q000254:growth & development*; D061249:Batch Cell Culture Techniques; D056804:Biofuels; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D002188:Cannabis / Q000737:chemistry*; D048429:Cell Size / Q000187:drug effects; D004951:Esterification / Q000187:drug effects; D004952:Esters / Q000378:metabolism; D066292:Lipid Droplets / Q000737:chemistry; D008055:Lipids / Q000096:biosynthesis*; D010802:Phylogeny; D010936:Plant Extracts / Q000494:pharmacology*; D012015:Reference Standards; D012639:Seeds / Q000737:chemistry*; D013010:Sonication; D017550:Spectroscopy, Fourier Transform Infrared; D014280:Triglycerides / Q000378:metabolism
chemical_list: D056804:Biofuels; D004952:Esters; D008055:Lipids; D010936:Plant Extracts; D014280:Triglycerides
keywords: BODIPY; Biodiesel; Rhodosporidium kratochvilovae HIMPA1; Sonicated hemp seed aqueous extract; Triacylglyceride