title: Differential DNA and RNA sequence discrimination by PNA having charged side chains.
abstract: PNA sequences modified with charged side chains were evaluated for base-pairing sequence selectivity under physiological conditions. PNA having negatively charged aspartic acid side chains shows higher selectivity with RNA, while PNA having positively charged lysine side chains shows higher selectivity with DNA. These observations provide insight into the binding selectivity of modified PNA in antisense and antigene applications.
authors.affiliation: Department of Chemistry, University of Utah, 315 South 1400 East, Salt Lake City, UT 84112, USA; Center for Cell and Genome Science, University of Utah, 257 South 1400 East, Salt Lake City, UT 84112, USA.
authors.forename: N Tilani S
authors.initials: NT
authors.lastname: De Costa
authors.affiliation: Department of Chemistry, University of Utah, 315 South 1400 East, Salt Lake City, UT 84112, USA; Center for Cell and Genome Science, University of Utah, 257 South 1400 East, Salt Lake City, UT 84112, USA. Electronic address: heemstra@chem.utah.edu.
authors.forename: Jennifer M
authors.initials: JM
authors.lastname: Heemstra
mesh_terms: D004247:DNA / Q000737:chemistry* / Q000235:genetics; D009841:Oligonucleotides / Q000737:chemistry / Q000235:genetics; D020135:Peptide Nucleic Acids / Q000737:chemistry* / Q000235:genetics; D012313:RNA / Q000737:chemistry*
chemical_list: D009841:Oligonucleotides; D020135:Peptide Nucleic Acids; D012313:RNA; D004247:DNA
keywords: Antisense; Electrostatic interactions; Nucleic acid biophysics; Oligonucleotides; Peptide nucleic acid