title: Energy Balance Regulating Neuropeptides Are Expressed through Pregnancy and Regulated by Interleukin-6 Deficiency in Mouse Placenta.
abstract: The placenta produces a number of signaling molecules including metabolic and reproductive hormones as well as several inflammatory mediators. Among them, Interleukin-6 (IL-6), a well-known immune and metabolic regulator, acts peripherally modulating metabolic function and centrally increasing energy expenditure and reducing body fat. IL-6 interacts with key hypothalamic neuropeptidergic systems controlling energy homeostasis such as those producing the orexigenic/anabolic: neuropeptide Y (NPY) and agouti-related peptide (AgRP) and anorectic/catabolic neuropeptides: proopiomelanocortin (POMC) and cocaine and amphetamine regulated transcript (CART). Human and rat placenta have been identified as source of these neuropeptides, but their expression and regulation in murine placental tissues remain unknown. Therefore, placental mRNA levels of IL-6, NPY, AgRP, POMC, and CART at different pregnancy stages (gestational days 13, 15, and 18) were analyzed by real time PCR, as were the effect of IL-6 deficiency (IL-6 knockout mice) on their placental expression. Our results showed that placenta-derived neuropeptides were regulated by gestational age and IL-6 throughout the second half of mouse pregnancy. These data suggest that IL-6 may participate in the fine tune control of energy balance during pregnancy by extending its action as a metabolic signal to the main organ at the fetomaternal interface: the placenta.
authors.affiliation: Department of Physiology, Research Center of Molecular Medicine and Chronic Diseases (CIMUS), University of Santiago de Compostela, Avenida de Barcelona s/n, 15782 Santiago de Compostela, Spain ; Instituto de Investigación Sanitaria de Santiago de Compostela (IDIS), 15706 Santiago de Compostela, Spain ; CIBER Fisiopatología Obesidad y Nutrición (CB06/03), Instituto de Salud Carlos III (ISCIII), Ministerio de Economía y Competitividad (MINECO), 15706 Santiago de Compostela, Spain.
authors.forename: Patricia
authors.initials: P
authors.lastname: Pazos
authors.affiliation: Department of Physiology, Research Center of Molecular Medicine and Chronic Diseases (CIMUS), University of Santiago de Compostela, Avenida de Barcelona s/n, 15782 Santiago de Compostela, Spain ; Instituto de Investigación Sanitaria de Santiago de Compostela (IDIS), 15706 Santiago de Compostela, Spain.
authors.forename: Luis
authors.initials: L
authors.lastname: Lima
authors.affiliation: Department of Physiology, Research Center of Molecular Medicine and Chronic Diseases (CIMUS), University of Santiago de Compostela, Avenida de Barcelona s/n, 15782 Santiago de Compostela, Spain ; Instituto de Investigación Sanitaria de Santiago de Compostela (IDIS), 15706 Santiago de Compostela, Spain ; CIBER Fisiopatología Obesidad y Nutrición (CB06/03), Instituto de Salud Carlos III (ISCIII), Ministerio de Economía y Competitividad (MINECO), 15706 Santiago de Compostela, Spain.
authors.forename: Carlos
authors.initials: C
authors.lastname: Diéguez
authors.affiliation: Department of Physiology, Research Center of Molecular Medicine and Chronic Diseases (CIMUS), University of Santiago de Compostela, Avenida de Barcelona s/n, 15782 Santiago de Compostela, Spain ; Instituto de Investigación Sanitaria de Santiago de Compostela (IDIS), 15706 Santiago de Compostela, Spain ; CIBER Fisiopatología Obesidad y Nutrición (CB06/03), Instituto de Salud Carlos III (ISCIII), Ministerio de Economía y Competitividad (MINECO), 15706 Santiago de Compostela, Spain.
authors.forename: María C
authors.initials: MC
authors.lastname: García
references.citation: Placenta. 2011 Feb;32(2):121-7
references.pmid: 21144584
references.citation: Am J Physiol Endocrinol Metab. 2008 May;294(5):E827-32
references.pmid: 18285524
references.citation: Brain Res Mol Brain Res. 2001 Jun 20;90(2):93-100
references.pmid: 11406287
references.citation: Nat Neurosci. 2012 Oct;15(10):1350-5
references.pmid: 23007190
references.citation: J Clin Endocrinol Metab. 1999 Aug;84(8):2755-8
references.pmid: 10443674
references.citation: Front Endocrinol (Lausanne). 2012 Mar 05;3:26
references.pmid: 22649413
references.citation: Cytokine. 2013 Jan;61(1):245-50
references.pmid: 23103122
references.citation: J Neuroendocrinol. 2011 Aug;23(8):675-86
references.pmid: 21564350
references.citation: J Neuroendocrinol. 2010 Jul;22(7):805-17
references.pmid: 20456605
references.citation: Am J Physiol Regul Integr Comp Physiol. 2006 Sep;291(3):R551-7
references.pmid: 16455769
references.citation: Placenta. 2013 Jul;34(7):559-66
references.pmid: 23639575
references.citation: Anim Reprod Sci. 2006 Nov;96(1-2):54-65
references.pmid: 16413151
references.citation: PLoS One. 2013;8(1):e52416
references.pmid: 23382817
references.citation: PLoS Biol. 2010 Aug 24;8(8):
references.pmid: 20808781
references.citation: Clin Exp Immunol. 2004 Jul;137(1):65-73
references.pmid: 15196245
references.citation: J Clin Endocrinol Metab. 2003 Sep;88(9):4379-83
references.pmid: 12970313
references.citation: Endocrinology. 2004 Jun;145(6):2680-6
references.pmid: 14988384
references.citation: Proc Nutr Soc. 2007 Feb;66(1):131-55
references.pmid: 17343779
references.citation: J Neuroendocrinol. 2013 Jun;25(6):580-9
references.pmid: 23414303
references.citation: J Pregnancy. 2012;2012:179827
references.pmid: 23304511
references.citation: Diabetologia. 2009 Oct;52(10):2159-68
references.pmid: 19644669
references.citation: J Neurosci. 2002 Jun 15;22(12):5091-9
references.pmid: 12077204
references.citation: Proc Natl Acad Sci U S A. 1997 Sep 30;94(20):11073-8
references.pmid: 9380761
references.citation: Nutr Neurosci. 2006 Feb-Apr;9(1-2):33-40
references.pmid: 16910168
references.citation: J Neuroendocrinol. 2012 Jun;24(6):930-43
references.pmid: 22295972
references.citation: Nature. 1994 Mar 24;368(6469):339-42
references.pmid: 8127368
references.citation: Reprod Biol Endocrinol. 2008 Apr 02;6:14
references.pmid: 18384674
references.citation: Endocrinology. 1986 Jun;118(6):2382-9
references.pmid: 2422019
references.citation: Eur J Endocrinol. 2000 May;142(5):533-6
references.pmid: 10802534
references.citation: J Neurosci. 2009 Jan 14;29(2):359-70
references.pmid: 19144836
references.citation: Hum Reprod Update. 2003 Nov-Dec;9(6):531-9
references.pmid: 14714590
references.citation: PLoS One. 2009;4(3):e4815
references.pmid: 19283075
references.citation: Endocrinology. 2005 Oct;146(10):4192-9
references.pmid: 16002529
references.citation: J Clin Endocrinol Metab. 2005 Jul;90(7):4276-86
references.pmid: 15855268
references.citation: Front Endocrinol (Lausanne). 2012 Feb 27;3:36
references.pmid: 22654862
references.citation: Horm Behav. 2007 Nov;52(4):508-19
references.pmid: 17706967
references.citation: J Clin Endocrinol Metab. 2003 Mar;88(3):1394-7
references.pmid: 12629135
references.citation: Growth Horm IGF Res. 2003 Aug;13 Suppl A:S28-32
references.pmid: 12914723
references.citation: Int J Pept. 2011;2011:969818
references.pmid: 22121381
references.citation: Nature. 2012 Nov 15;491(7424):357-63
references.pmid: 23151578
references.citation: Placenta. 2013 Jul;34(7):567-73
references.pmid: 23623485
references.citation: Biochem Biophys Res Commun. 2002 Apr 26;293(1):560-5
references.pmid: 12054638
references.citation: Int J Biol Sci. 2012;8(9):1254-66
references.pmid: 23136554
references.citation: J Clin Endocrinol Metab. 2000 Sep;85(9):3356-64
references.pmid: 10999834
references.citation: Methods. 2001 Dec;25(4):402-8
references.pmid: 11846609
references.citation: J Clin Endocrinol Metab. 2013 Jun;98(6):2431-41
references.pmid: 23539720
references.citation: PLoS One. 2013 Aug 28;8(8):e72339
references.pmid: 24015235
references.citation: Endocrinology. 1997 Oct;138(10):4501-4
references.pmid: 9322972
references.citation: Brain Behav Immun. 2010 Jan;24(1):119-26
references.pmid: 19748567
references.citation: Front Neuroendocrinol. 2012 Oct;33(4):342-63
references.pmid: 22981653
references.citation: J Cell Mol Med. 2009 Feb;13(2):388-97
references.pmid: 18410529
references.citation: J Mol Endocrinol. 2011 Aug 30;47(2):157-65
references.pmid: 21697073
references.citation: Nat Med. 2002 Jan;8(1):75-9
references.pmid: 11786910
references.citation: Endocrinology. 2008 Sep;149(9):4534-43
references.pmid: 18499762
references.citation: Am J Physiol Cell Physiol. 2009 Jul;297(1):C140-51
references.pmid: 19420002
references.citation: Horm Metab Res. 2000 May;32(5):164-8
references.pmid: 10871155
references.citation: Diabetes. 2006 Sep;55(9):2554-61
references.pmid: 16936204
references.citation: Diabetes. 2010 May;59(5):1161-70
references.pmid: 20150288
references.citation: Neuroendocrinology. 2007;86(3):175-82
references.pmid: 17898535
references.citation: Endocrinology. 1992 Jul;131(1):14-20
references.pmid: 1611993
references.citation: Dis Model Mech. 2011 Nov;4(6):733-45
references.pmid: 22065844
references.citation: Placenta. 2006 Aug;27(8):794-8
references.pmid: 16242770
references.citation: J Neuroendocrinol. 2009 Jul;21(7):620-8
references.pmid: 19490366
references.citation: Am J Physiol Endocrinol Metab. 2011 May;300(5):E858-69
references.pmid: 21343543