title: Confined trisomy 8 mosaicism of meiotic origin: a rare cause of aneuploidy in childhood cancer.
abstract: Whether chromosome abnormalities observed in tumor cells may in some cases reflect low-grade somatic mosaicism for anomalies present already at zygote formation, rather than acquired somatic mutations, has for long remained a speculation. We here report a patient with Wilms tumor, where constitutional somatic mosaicism of trisomy 8 was detected in a previously healthy 2 ½-year-old boy. Single Nucleotide Polymorphism (SNP) array analysis of tumor tissue revealed a complex distribution of allele frequencies for chromosome 8 that could not be explained solely by mitotic events. Combined analysis of allele frequencies, chromosome banding, and fluorescence in situ hybridization revealed that the majority of tumor cells contained four copies of chromosome 8, with three distinct haplotypes at a 2:1:1 ratio. Because the patient had not been subject to organ transplantation, these findings indicated that the tumor karyotype evolved from a cell with trisomy 8 of meiotic origin, with subsequent somatic gain of one additional chromosome copy. Haplotype analysis was consistent with trisomy 8 through nondisjunction at meiosis I. Matched normal renal tissue or peripheral blood did not contain detectable amounts of cells with trisomy 8, consistent with the complete lack of mosaic trisomy 8 syndrome features in the patient. This case provides proof of principle for the hypothesis that tumor genotypes may in rare cases reflect meiotic rather than mitotic events, also in patients lacking syndromic features. © 2014 Wiley Periodicals, Inc.
authors.affiliation: Department of Clinical Genetics, Lund University, University and Regional Laboratories, Lund, Sweden.
authors.forename: Anders
authors.initials: A
authors.lastname: Valind
authors.forename: Niklas
authors.initials: N
authors.lastname: Pal
authors.forename: Jurate
authors.initials: J
authors.lastname: Asmundsson
authors.forename: David
authors.initials: D
authors.lastname: Gisselsson
authors.forename: Linda
authors.initials: L
authors.lastname: Holmquist Mengelbier
mesh_terms: D000782:Aneuploidy; D002675:Child, Preschool; D002898:Chromosomes, Human, Pair 8 / Q000235:genetics; D006801:Humans; D007621:Karyotyping; D007680:Kidney Neoplasms / Q000235:genetics*; D008297:Male; D008540:Meiosis; D009030:Mosaicism; D020641:Polymorphism, Single Nucleotide; D014314:Trisomy / Q000235:genetics*; D024182:Uniparental Disomy / Q000235:genetics*; D009396:Wilms Tumor / Q000235:genetics*