title: MicroRNA clusters: dysregulation in lung adenocarcinoma and COPD.
abstract: Lung adenocarcinoma and chronic obstructive pulmonary disease (COPD) are pulmonary diseases that share common aetiological factors (tobacco smoking) and probable dysregulated pathways. MicroRNAs (miRNAs) play an essential role in regulating numerous physiological and pathological processes. The purpose of this study was to assess global miRNA expression patterns in patients with COPD and/or adenocarcinoma to elucidate distinct regulatory networks involved in the pathogenesis of these two smoking-related diseases. Expression of 381 miRNAs was quantified by TaqMan Human MicroRNA A Array v2.0 in bronchoalveolar lavage fluid samples from 87 patients classified into four groups: COPD, adenocarcinoma, adenocarcinoma with COPD, and control (neither COPD nor adenocarcinoma). 11 differentially expressed miRNAs were randomly selected for validation in an independent cohort of 40 patients. Distinct miRNA expression profiles were identified and validated for each pathological group, involving 66 differentially expressed miRNAs. Four miRNA clusters (the mir-17-92 cluster and its paralogues, mir-106a-363 and mir-106b-25; and the miR-192-194 cluster) were upregulated in patients with adenocarcinoma and one miRNA cluster (miR-132-212) was upregulated in patients with COPD. These results contribute to unravelling miRNA-controlled networks involved in the pathogenesis of adenocarcinoma and COPD, and provide new tools of potential use as biomarkers for diagnosis and/or therapeutic purposes.
authors.affiliation: Instituto de Biomedicina de Sevilla (IBIS), HUVR, CSIC, Universidad de Sevilla, Seville, Spain.
authors.forename: Sonia
authors.initials: S
authors.lastname: Molina-Pinelo
authors.affiliation: Instituto de Biomedicina de Sevilla (IBIS), HUVR, CSIC, Universidad de Sevilla, Seville, Spain.
authors.forename: M Dolores
authors.initials: MD
authors.lastname: Pastor
authors.affiliation: Instituto de Biomedicina de Sevilla (IBIS), HUVR, CSIC, Universidad de Sevilla, Seville, Spain.
authors.forename: Rocío
authors.initials: R
authors.lastname: Suarez
authors.affiliation: Pneumology Dept, Hospital Universitario Virgen del Rocío, Seville, Spain.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Romero-Romero
authors.affiliation: Medical Oncology Dept, Hospital Universitario Virgen del Rocío, Seville, Spain.
authors.forename: Miriam
authors.initials: M
authors.lastname: González De la Peña
authors.affiliation: Instituto de Biomedicina de Sevilla (IBIS), HUVR, CSIC, Universidad de Sevilla, Seville, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Salinas
authors.affiliation: Instituto de Biomedicina de Sevilla (IBIS), HUVR, CSIC, Universidad de Sevilla, Seville, Spain Medical Oncology Dept, Hospital Universitario Virgen del Rocío, Seville, Spain.
authors.forename: Rocío
authors.initials: R
authors.lastname: García-Carbonero
authors.affiliation: Medical Oncology Dept, Hospital Universitario Virgen del Rocío, Seville, Spain.
authors.forename: María José
authors.initials: MJ
authors.lastname: De Miguel
authors.affiliation: Instituto de Biomedicina de Sevilla (IBIS), HUVR, CSIC, Universidad de Sevilla, Seville, Spain.
authors.forename: Francisco
authors.initials: F
authors.lastname: Rodríguez-Panadero
authors.affiliation: Instituto de Biomedicina de Sevilla (IBIS), HUVR, CSIC, Universidad de Sevilla, Seville, Spain.
authors.forename: Amancio
authors.initials: A
authors.lastname: Carnero
authors.affiliation: Instituto de Biomedicina de Sevilla (IBIS), HUVR, CSIC, Universidad de Sevilla, Seville, Spain Medical Oncology Dept, Hospital Universitario Virgen del Rocío, Seville, Spain lpazares@hotmail.com.
authors.forename: Luis
authors.initials: L
authors.lastname: Paz-Ares
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000378:metabolism; D000077192:Adenocarcinoma of Lung; D000328:Adult; D000368:Aged; D000465:Algorithms; D001992:Bronchoalveolar Lavage Fluid; D002874:Chromosome Mapping; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D053263:Gene Regulatory Networks; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D008875:Middle Aged; D011446:Prospective Studies; D029424:Pulmonary Disease, Chronic Obstructive / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D035683:MicroRNAs