title: Polarization of ILC2s in peripheral blood might contribute to immunosuppressive microenvironment in patients with gastric cancer.
abstract: Newly identified nuocytes or group 2 innate lymphoid cells (ILC2s) play an important role in Th2 cell mediated immunity such as protective immune responses to helminth parasites, allergic asthma, and chronic rhinosinusitis. However, the contributions of ILC2s in the occurrence and development of cancer remain unknown. Our previous study found that there was a predominant Th2 phenotype in patients with gastric cancer. In this study, the ILC2s related genes or molecules in PBMC from patients with gastric cancer were measured, and the potential correlation between them was analyzed. The expression levels of RORα, GATA3, T1/ST2, IL-17RB, CRTH2, IL-33, IL-5, and IL-4 mRNA were significantly increased in patients, but no significant changes were found in ICOS, CD45, and IL-13 expression, and there was a positive correlation between RORα or IL-13 and other related factors, such as ICOS and CD45. The increased frequency of ILC2s was also found in PBMC of patients by flow cytometry. In addition, the mRNA of Arg1 and iNOS were also significantly increased in patients. These results suggested that there are polarized ILC2s in gastric cancer patients which might contribute to immunosuppressive microenvironment and closely related to the upregulation of MDSCs and M2 macrophages.
authors.affiliation: Department of Immunology, School of Medical Science and Laboratory Medicine, Jiangsu University, Xuefu Road 301, Zhenjiang 212013, China.
authors.forename: Qingli
authors.initials: Q
authors.lastname: Bie
authors.affiliation: Department of Immunology, School of Medical Science and Laboratory Medicine, Jiangsu University, Xuefu Road 301, Zhenjiang 212013, China.
authors.forename: Pan
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Department of Immunology, School of Medical Science and Laboratory Medicine, Jiangsu University, Xuefu Road 301, Zhenjiang 212013, China.
authors.forename: Zhaoliang
authors.initials: Z
authors.lastname: Su
authors.affiliation: Department of Immunology, School of Medical Science and Laboratory Medicine, Jiangsu University, Xuefu Road 301, Zhenjiang 212013, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Zheng
authors.affiliation: Department of Immunology, School of Medical Science and Laboratory Medicine, Jiangsu University, Xuefu Road 301, Zhenjiang 212013, China.
authors.forename: Xinyu
authors.initials: X
authors.lastname: Ying
authors.affiliation: Department of Immunology, School of Medical Science and Laboratory Medicine, Jiangsu University, Xuefu Road 301, Zhenjiang 212013, China.
authors.forename: Yumin
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Immunology, School of Medical Science and Laboratory Medicine, Jiangsu University, Xuefu Road 301, Zhenjiang 212013, China.
authors.forename: Huijian
authors.initials: H
authors.lastname: Yang
authors.affiliation: Institute of Oncology, The Affiliated Hospital of Jiangsu University, Zhenjiang 212001, China.
authors.forename: Deyu
authors.initials: D
authors.lastname: Chen
authors.affiliation: Department of Immunology, School of Medical Science and Laboratory Medicine, Jiangsu University, Xuefu Road 301, Zhenjiang 212013, China.
authors.forename: Shengjun
authors.identifier: 0000-0001-6584-1183
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Immunology, School of Medical Science and Laboratory Medicine, Jiangsu University, Xuefu Road 301, Zhenjiang 212013, China.
authors.forename: Huaxi
authors.initials: H
authors.lastname: Xu
mesh_terms: D000328:Adult; D000368:Aged; D016207:Cytokines / Q000235:genetics / Q000276:immunology; D005260:Female; D050990:GATA3 Transcription Factor / Q000235:genetics / Q000276:immunology; D015870:Gene Expression / Q000276:immunology*; D006801:Humans; D007113:Immunity, Innate; D016130:Immunophenotyping; D060889:Inducible T-Cell Co-Stimulator Protein / Q000235:genetics / Q000276:immunology; D017493:Leukocyte Common Antigens / Q000235:genetics / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology* / Q000473:pathology; D008264:Macrophages / Q000276:immunology / Q000473:pathology; D008297:Male; D008875:Middle Aged; D057094:Nuclear Receptor Subfamily 1, Group F, Member 1 / Q000235:genetics / Q000276:immunology; D013274:Stomach Neoplasms / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D018418:Th2 Cells / Q000276:immunology / Q000473:pathology; D059016:Tumor Microenvironment / Q000276:immunology*
chemical_list: D016207:Cytokines; D050990:GATA3 Transcription Factor; C494749:GATA3 protein, human; C556692:ICOS protein, human; D060889:Inducible T-Cell Co-Stimulator Protein; D057094:Nuclear Receptor Subfamily 1, Group F, Member 1; D017493:Leukocyte Common Antigens; C516769:PTPRC protein, human
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