title: In vivo near-infrared photothermal therapy and computed tomography imaging of cancer cells using novel tungsten-based theranostic probe.
abstract: Photothermal therapy, as a physical therapeutic technique to kill cancer, has generated a great deal of interest. Photothermal agents hence play a critical role in this modern therapy. We report the use of transition metal oxides as photothermal agents based on PEGylated WO3-x nanoparticles. The well-prepared nanoparticles presented effective results during photothermal therapy both in vitro and in vivo by using near-IR laser irradiation (980 nm, 0.5 W cm(-2)). The tumor cells were effectively damaged using low power density during a short irradiation time without destroying healthy tissues. In vitro results of photothermal therapy with PEGylated WO3-x nanoparticles proved to be effective on 4T1 murine breast cancer cells via a confocal microscopy method and MTT assay. In vivo results were further confirmed by hematoxylin and eosin (H & E) histological staining. Additionally, PEGylated WO3-x nanoparticles were shown to be effective as a CT imaging contrast agent on a tumor-bearing mouse model. Our results suggest that this generation of PEGylated WO3-x nanoparticles can potentially be used in oncological CT imaging and photothermal therapy.
authors.affiliation: Department of Radiology, The Second Hospital of Jilin University, Changchun, 130041 P. R. China. drliujh@yahoo.com Hanxueli6613@sina.cn drlihp@yahoo.com.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Liu
authors.forename: Jianguo
authors.initials: J
authors.lastname: Han
authors.forename: Zhichen
authors.initials: Z
authors.lastname: Kang
authors.forename: Reza
authors.initials: R
authors.lastname: Golamaully
authors.forename: Nannan
authors.initials: N
authors.lastname: Xu
authors.forename: Hongpeng
authors.initials: H
authors.lastname: Li
authors.forename: Xueli
authors.initials: X
authors.lastname: Han
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D004195:Disease Models, Animal; D006979:Hyperthermia, Induced; D007259:Infrared Rays; D007834:Lasers; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000627:therapeutic use / Q000633:toxicity; D051379:Mice; D018613:Microscopy, Confocal; D009369:Neoplasms / Q000000981:diagnostic imaging / Q000473:pathology / Q000628:therapy; D019301:Oleic Acid / Q000737:chemistry; D010789:Phototherapy; D011092:Polyethylene Glycols / Q000737:chemistry; D014057:Tomography, X-Ray Computed; D014414:Tungsten / Q000737:chemistry*
chemical_list: D019301:Oleic Acid; D011092:Polyethylene Glycols; D014414:Tungsten