title: Diffusion tensor imaging analysis of sequential spreading of disease in amyotrophic lateral sclerosis confirms patterns of TDP-43 pathology.
abstract: Diffusion tensor imaging can identify amyotrophic lateral sclerosis-associated patterns of brain alterations at the group level. Recently, a neuropathological staging system for amyotrophic lateral sclerosis has shown that amyotrophic lateral sclerosis may disseminate in a sequential regional pattern during four disease stages. The objective of the present study was to apply a new methodological diffusion tensor imaging-based approach to automatically analyse in vivo the fibre tracts that are prone to be involved at each neuropathological stage of amyotrophic lateral sclerosis. Two data samples, consisting of 130 diffusion tensor imaging data sets acquired at 1.5 T from 78 patients with amyotrophic lateral sclerosis and 52 control subjects; and 55 diffusion-tensor imaging data sets at 3.0 T from 33 patients with amyotrophic lateral sclerosis and 22 control subjects, were analysed by a tract of interest-based fibre tracking approach to analyse five tracts that become involved during the course of amyotrophic lateral sclerosis: the corticospinal tract (stage 1); the corticorubral and the corticopontine tracts (stage 2); the corticostriatal pathway (stage 3); the proximal portion of the perforant path (stage 4); and two reference pathways. The statistical analyses of tracts of interest showed differences between patients with amyotrophic lateral sclerosis and control subjects for all tracts. The significance level of the comparisons at the group level was lower, the higher the disease stage with corresponding involved fibre tracts. Both the clinical phenotype as assessed by the amyotrophic lateral sclerosis functional rating scale-revised and disease duration correlated significantly with the resulting staging scheme. In summary, the tract of interest-based technique allowed for individual analysis of predefined tract structures, thus making it possible to image in vivo the disease stages in amyotrophic lateral sclerosis. This approach can be used not only for individual clinical work-up purposes, but enlarges the spectrum of potential non-invasive surrogate markers as a neuroimaging-based read-out for amyotrophic lateral sclerosis studies within a clinical context.
authors.affiliation: 1 Department of Neurology, University of Ulm, Ulm, Germany jan.kassubek@uni-ulm.de.
authors.forename: Jan
authors.initials: J
authors.lastname: Kassubek
authors.affiliation: 1 Department of Neurology, University of Ulm, Ulm, Germany.
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Müller
authors.affiliation: 2 Section Clinical Neuroanatomy, Department of Neurology, University of Ulm, Ulm, Germany.
authors.forename: Kelly
authors.initials: K
authors.lastname: Del Tredici
authors.affiliation: 1 Department of Neurology, University of Ulm, Ulm, Germany2 Section Clinical Neuroanatomy, Department of Neurology, University of Ulm, Ulm, Germany.
authors.forename: Johannes
authors.initials: J
authors.lastname: Brettschneider
authors.affiliation: 1 Department of Neurology, University of Ulm, Ulm, Germany.
authors.forename: Elmar H
authors.initials: EH
authors.lastname: Pinkhardt
authors.affiliation: 1 Department of Neurology, University of Ulm, Ulm, Germany.
authors.forename: Dorothée
authors.initials: D
authors.lastname: Lulé
authors.affiliation: 1 Department of Neurology, University of Ulm, Ulm, Germany.
authors.forename: Sarah
authors.initials: S
authors.lastname: Böhm
authors.affiliation: 2 Section Clinical Neuroanatomy, Department of Neurology, University of Ulm, Ulm, Germany.
authors.forename: Heiko
authors.initials: H
authors.lastname: Braak
authors.affiliation: 1 Department of Neurology, University of Ulm, Ulm, Germany.
authors.forename: Albert C
authors.initials: AC
authors.lastname: Ludolph
mesh_terms: D000328:Adult; D000368:Aged; D000690:Amyotrophic Lateral Sclerosis / Q000473:pathology* / Q000503:physiopathology; D016880:Anisotropy; D004268:DNA-Binding Proteins / Q000378:metabolism; D056324:Diffusion Tensor Imaging; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods; D008297:Male; D008875:Middle Aged; D011712:Pyramidal Tracts / Q000503:physiopathology*
chemical_list: D004268:DNA-Binding Proteins
keywords: amyotrophic lateral sclerosis; diffusion tensor imaging; fractional anisotropy; magnetic resonance imaging; motor neuron disease; staging