title: Drosophila microbiota modulates host metabolic gene expression via IMD/NF-κB signaling.
abstract: Most metazoans engage in mutualistic interactions with their intestinal microbiota. Despite recent progress the molecular mechanisms through which microbiota exerts its beneficial influences on host physiology are still largely uncharacterized. Here we use axenic Drosophila melanogaster adults associated with a standardized microbiota composed of a defined set of commensal bacterial strains to study the impact of microbiota association on its host transcriptome. Our results demonstrate that Drosophila microbiota has a marked impact on the midgut transcriptome and promotes the expression of genes involved in host digestive functions and primary metabolism. We identify the IMD/Relish signaling pathway as a central regulator of this microbiota-mediated transcriptional response and we reveal a marked transcriptional trade-off between the midgut response to its beneficial microbiota and to bacterial pathogens. Taken together our results indicate that microbiota association potentiates host nutrition and host metabolic state, two key physiological parameters influencing host fitness. Our work paves the way to subsequent mechanistic studies to reveal how these microbiota-dependent transcriptional signatures translate into host physiological benefits.
authors.forename: Berra
authors.initials: B
authors.lastname: Erkosar
authors.affiliation: Institut de Génomique Fonctionnelle de Lyon, Ecole Normale Supérieure de Lyon, Centre National de la Recherche Scientifique, Université Claude Bernard Lyon-1, Lyon, France; Aix-Marseille Université, Centre National de la Recherche Scientifique, Institut de Biologie du Développement de Marseille-Luminy, Marseille, France.
authors.forename: Berra
authors.initials: B
authors.lastname: Erkosar Combe
authors.affiliation: Aix-Marseille Université, Centre National de la Recherche Scientifique, Institut de Biologie du Développement de Marseille-Luminy, Marseille, France.
authors.forename: Arnaud
authors.initials: A
authors.lastname: Defaye
authors.affiliation: Institut de Génomique Fonctionnelle de Lyon, Ecole Normale Supérieure de Lyon, Centre National de la Recherche Scientifique, Université Claude Bernard Lyon-1, Lyon, France.
authors.forename: Noémie
authors.initials: N
authors.lastname: Bozonnet
authors.affiliation: Technological Advances for Genomics and Clinics, Institut National de la Santé et de la Recherche Médicale, Aix-Marseille Université, Marseille, France.
authors.forename: Denis
authors.initials: D
authors.lastname: Puthier
authors.affiliation: Aix-Marseille Université, Centre National de la Recherche Scientifique, Institut de Biologie du Développement de Marseille-Luminy, Marseille, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Royet
authors.affiliation: Institut de Génomique Fonctionnelle de Lyon, Ecole Normale Supérieure de Lyon, Centre National de la Recherche Scientifique, Université Claude Bernard Lyon-1, Lyon, France; Aix-Marseille Université, Centre National de la Recherche Scientifique, Institut de Biologie du Développement de Marseille-Luminy, Marseille, France.
authors.forename: François
authors.initials: F
authors.lastname: Leulier
mesh_terms: D000818:Animals; D029721:Drosophila Proteins / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000276:immunology / Q000382:microbiology*; D005260:Female; D005786:Gene Expression Regulation; D007107:Immune System; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000382:microbiology; D064307:Microbiota; D010641:Phenotype; D015398:Signal Transduction; D014157:Transcription Factors / Q000378:metabolism*; D059467:Transcriptome
chemical_list: D029721:Drosophila Proteins; C101626:Rel protein, Drosophila; D014157:Transcription Factors; C439909:imd protein, Drosophila
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