title: Seafood-like flavour obtained from the enzymatic hydrolysis of the protein by-products of seaweed (Gracilaria sp.).
abstract: An enzymatic bromelain seaweed protein hydrolysate (eb-SWPH) was characterised as the precursor for thermally processed seafood flavour. Seaweed (Gracilaria fisheri) protein after agar extraction was hydrolysed using bromelain (enzyme activity=119,325 U/g) at 0-20% (w/w) for 0.5-24 h. Optimal hydrolysis conditions were determined using response surface methodology. The proposed model took into account the interaction effect of the enzyme concentration and hydrolysis time on the physicochemical properties and volatile components of eb-SWPH. The optimal hydrolysis conditions for the production of eb-SWPH were 10% bromelain for 3h, which resulted in a 38.15% yield and a 62.91% degree of hydrolysis value. Three free amino acids, arginine, lysine, and leucine, were abundant in the best hydrolysate. Ten volatile flavours of the best eb-SWPH were identified using gas chromatography/mass spectrometry. The predominant odourants were hexanal, hexanoic acid, nonanoic acid, and dihydroactinidiolide. The thermally processed seafood flavour produced from eb-SWPH exhibited a roasted seafood-like flavouring.
authors.affiliation: Division of Biochemical Technology, School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, 49 Tientalay 25 Rd., Takham, Bangkhuntien, Bangkok 10150, Thailand. Electronic address: nutta.lao@kmutt.ac.th.
authors.forename: Natta
authors.initials: N
authors.lastname: Laohakunjit
authors.affiliation: Division of Biochemical Technology, School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, 49 Tientalay 25 Rd., Takham, Bangkhuntien, Bangkok 10150, Thailand.
authors.forename: Orrapun
authors.initials: O
authors.lastname: Selamassakul
authors.affiliation: Division of Biochemical Technology, School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, 49 Tientalay 25 Rd., Takham, Bangkhuntien, Bangkok 10150, Thailand.
authors.forename: Orapin
authors.initials: O
authors.lastname: Kerdchoechuen
mesh_terms: D001963:Bromelains / Q000378:metabolism*; D006868:Hydrolysis; D012624:Seaweed / Q000737:chemistry*; D013649:Taste
chemical_list: D001963:Bromelains
keywords: Bromelain; Enzymatic hydrolysis; Seafood-like flavour; Seaweed; Thermal processed flavour