title: Structure of the O-polysaccharide from the lipopolysaccharide of Providencia alcalifaciens O33.
abstract: Mild acid degradation of the lipopolysaccharide from Providencia alcalifaciens O33 resulted in an O-polysaccharide along with core and O-unit-bearing core oligosaccharides. Composition of the oligosaccharides was inferred by ESI mass spectrometry. Based on sugar and methylation analyses, Smith degradation and (1)H and (13)C NMR spectroscopy data, the following structure of the tetrasaccharide O-unit of the O-polysaccharide was established: Another O-polysaccharide structure has been reported earlier for Providencia stuartii О33 but later found to belong to a P. stuartii О52 strain.
authors.affiliation: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation. Electronic address: olga.ovchinnikova@gmail.com.
authors.forename: Olga G
authors.initials: OG
authors.lastname: Ovchinnikova
authors.affiliation: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Shashkov
authors.affiliation: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
authors.forename: Alexander O
authors.initials: AO
authors.lastname: Chizhov
authors.affiliation: Department of Immunobiology of Bacteria, Institute of Microbiology, Biotechnology and Immunology, University of Lodz, PL 90-237 Lodz, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Moryl
authors.affiliation: Department of Immunobiology of Bacteria, Institute of Microbiology, Biotechnology and Immunology, University of Lodz, PL 90-237 Lodz, Poland.
authors.forename: Antoni
authors.initials: A
authors.lastname: Rozalski
authors.affiliation: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
authors.forename: Yuriy A
authors.initials: YA
authors.lastname: Knirel
mesh_terms: D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D019081:O Antigens / Q000737:chemistry*; D011532:Providencia; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D019081:O Antigens
keywords: Bacterial polysaccharide structure; Lipopolysaccharide; O-Antigen; Providencia alcalifaciens