title: Rapid hybridization of chitosan-gold-antibodies via metal-free click in water-based systems: a model approach for naked-eye detectable antigen sensors.
abstract: A surface plasmon resonance (SPR) expression after hybridization of chitosan-gold nanoparticle-antibody (CS-AuNPs-Ab) based on: i) metal-free click chemistry, and, ii) in water system as an approach for a rapid antigen sensing, is proposed. The chitosan-hydroxybenzyl triazole complex enables us to carry out the conjugation of mPEG and trifluoromethylated oxanorbornadiene (OND) in water. CS-mPEG-OND further allows metal-free click to hybridize chitosan (CS) with azido-modified gold nanoparticles (azido-AuNPs) in aqueous solution at room temperature. The CS-mPEG-OND conjugated with LipL32 antibody (Ab) not only effectively binds with LipL32 antigen (Ag) but also performs the cycloaddition with azido-AuNPs to display a change in color within 2 min. The phenomenon leads to a simple and efficient naked-eye antigen detection technique.
authors.affiliation: The Petroleum and Petrochemical College, Chulalongkorn University, Bangkok, 10330, Thailand.
authors.forename: Jatesuda
authors.initials: J
authors.lastname: Jirawutthiwongchai
authors.forename: Gerald
authors.initials: G
authors.lastname: Draeger
authors.forename: Suwabun
authors.initials: S
authors.lastname: Chirachanchai
mesh_terms: D000906:Antibodies / Q000737:chemistry* / Q000276:immunology; D000941:Antigens / Q000032:analysis*; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D048271:Chitosan / Q000737:chemistry*; D057930:Click Chemistry; D003124:Colorimetry; D006046:Gold / Q000737:chemistry*; D007536:Isomerism; D053768:Metal Nanoparticles / Q000737:chemistry; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D014867:Water / Q000737:chemistry*
chemical_list: D000906:Antibodies; D000941:Antigens; D009838:Oligodeoxyribonucleotides; D014867:Water; D006046:Gold; D048271:Chitosan
keywords: antigen sensors; gold nanoparticles; metal-free click chemistry; oxanorbornadiene; water soluble chitosan