title: Towards personalized medicine mediated by in vitro virus-based interactome approaches.
abstract: We have developed a simple in vitro virus (IVV) selection system based on cell-free co-translation, using a highly stable and efficient mRNA display method. The IVV system is applicable to the high-throughput and comprehensive analysis of proteins and protein-ligand interactions. Huge amounts of genomic sequence data have been generated over the last decade. The accumulated genetic alterations and the interactome networks identified within cells represent a universal feature of a disease, and knowledge of these aspects can help to determine the optimal therapy for the disease. The concept of the "integrome" has been developed as a means of integrating large amounts of data. We have developed an interactome analysis method aimed at providing individually-targeted health care. We also consider future prospects for this system.
authors.affiliation: Division of Interactome Medical Sciences, the Institute of Medical Science, the University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan. ohashi@yokohamalken.or.jp.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Ohashi
authors.affiliation: Division of Interactome Medical Sciences, the Institute of Medical Science, the University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan. nekoneko@ims.u-tokyo.ac.jp.
authors.forename: Etsuko
authors.initials: E
authors.lastname: Miyamoto-Sato
mesh_terms: D002474:Cell-Free System; D023281:Genomics; D006801:Humans; D057285:Precision Medicine; D060066:Protein Interaction Maps; D011506:Proteins / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D014780:Viruses / Q000378:metabolism*
chemical_list: D011506:Proteins; D012333:RNA, Messenger
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