title: Antifungal and antioxidant activity of Crassocephalum bauchiense (Hutch.) Milne-Redh ethyl acetate extract and fractions (Asteraceae).
abstract: BACKGROUND
  Crassocephalum bauchiense is a flowering plant, found in the West Region of Cameroon. Previous studied has highlighted the antibacterial and the dermal toxicological safety as well as the immunomodulatory activities of the ethyl acetate extract of its dry leaves. As an extension of the previous researches, the current work has been undertaken to evaluate the in vitro antifungal and antioxidant activities of C. bauchiense dried leaves ethyl acetate extract and fractions.
  
  
  METHODS
  The extract was obtained by maceration in ethyl acetate and further fractionated into six fractions labeled F1 to F6 by flash chromatography. The antifungal activity of the extract and fractions against yeasts and dermatophytes was evaluated using broth microdilution method. Antioxidant activity was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH), nitric oxide (NO) and β-carotene - linoleic acid assays.
  
  
  RESULTS
  The extract (MIC = 0.125 - 4 mg/ml) was found to be more active on dermatophytes and yeasts compared to the fractions. The ethyl acetate extract and fractions exhibited strong scavenging activity on DPPH (CI50 = 28.57 - 389.38 μg/ml). The fractions F3 and F6 expressed best antioxidant activity on DPPH radicals compared to the crude extract.
  
  
  CONCLUSIONS
  The results of these findings clearly showed that C. bauchiense ethyl acetate extract has a significant antifungal and antioxidant activity. It is therefore a source of active compounds that might be used as antifungal and antioxidant agents.
authors.affiliation: Laboratory of Microbiology and Food Quality Control, Institute of Fisheries and Aquatic Sciences, University of Douala, P,O, Box 7236, Douala, Cameroon. moraysi@yahoo.fr.
authors.forename: Raymond Simplice
authors.initials: RS
authors.lastname: Mouokeu
authors.forename: Rosalie A Ngono
authors.initials: RA
authors.lastname: Ngane
authors.forename: Guy S S
authors.initials: GS
authors.lastname: Njateng
authors.forename: Monique O
authors.initials: MO
authors.lastname: Kamtchueng
authors.forename: Jules-Roger
authors.initials: JR
authors.lastname: Kuiate
mesh_terms: D000085:Acetates / Q000737:chemistry*; D000935:Antifungal Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D019659:Asteraceae / Q000737:chemistry*; D001419:Bacteria / Q000187:drug effects; D005591:Chemical Fractionation; D016166:Free Radical Scavengers / Q000494:pharmacology; D005658:Fungi / Q000187:drug effects; D019787:Linoleic Acid / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D010084:Oxidation-Reduction / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*
chemical_list: D000085:Acetates; D000935:Antifungal Agents; D000975:Antioxidants; D016166:Free Radical Scavengers; D010936:Plant Extracts; C007650:ethyl acetate; D019787:Linoleic Acid
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