title: Postintegration stability of the silkworm piggyBac transposon.
abstract: The piggyBac transposon is the most widely used vector for generating transgenic silkworms. The silkworm genome contains multiple piggyBac-like sequences that might influence the genetic stability of transgenic lines. To investigate the postintegration stability of piggyBac in silkworms, we used random insertion of the piggyBac [3 × p3 EGFP afm] vector to generate a W chromosome-linked transgenic silkworm, named W-T. Results of Southern blot and inverse PCR revealed the insertion of a single copy in the W chromosome of W-T at a standard TTAA insertion site. Investigation of 11 successive generations showed that all W-T females were EGFP positive and all males were EGFP negative; PCR revealed that the insertion site was unchanged in W-T offspring. These results suggested that endogenous piggyBac-like elements did not affect the stability of piggyBac inserted into the silkworm genome.
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, PR China; College of Biotechnology, Southwest University, Chongqing 400715, PR China.
authors.forename: Liang
authors.initials: L
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, PR China; College of Biotechnology, Southwest University, Chongqing 400715, PR China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, PR China; College of Biotechnology, Southwest University, Chongqing 400715, PR China.
authors.forename: Weiqiang
authors.initials: W
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, PR China; College of Biotechnology, Southwest University, Chongqing 400715, PR China.
authors.forename: Huizhen
authors.initials: H
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, PR China; College of Biotechnology, Southwest University, Chongqing 400715, PR China.
authors.forename: Zhengwen
authors.initials: Z
authors.lastname: Peng
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, PR China; College of Biotechnology, Southwest University, Chongqing 400715, PR China.
authors.forename: Yinghui
authors.initials: Y
authors.lastname: Dang
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, PR China; College of Biotechnology, Southwest University, Chongqing 400715, PR China.
authors.forename: Chunlin
authors.initials: C
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, PR China; College of Biotechnology, Southwest University, Chongqing 400715, PR China.
authors.forename: Ping
authors.initials: P
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, PR China; College of Biotechnology, Southwest University, Chongqing 400715, PR China. Electronic address: xiaqy@swu.edu.cn.
authors.forename: Qingyou
authors.initials: Q
authors.lastname: Xia
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics*; D001483:Base Sequence; D012831:Bombyx / Q000235:genetics*; D004251:DNA Transposable Elements / Q000235:genetics*; D005260:Female; D049452:Green Fluorescent Proteins / Q000235:genetics; D008297:Male; D019076:Transgenes
chemical_list: D004251:DNA Transposable Elements; D049452:Green Fluorescent Proteins
keywords: PiggyBac; Silkworm; Stability; Transgenic; W chromosome-linked