title: Generation of a monoclonal antibody against the extracellular domain of IGF-1R.
abstract: Type I insulin-like growth factor receptor (IGF-1R) is a receptor tyrosine kinase that is involved in the transformation of cells, cancer proliferation, and metastatic events in various types of cancer. The present study reports on the generation of a mouse monoclonal antibody (MAb) to IGF-1R using the mouse lymph node method. MAb 1B1, which reacts specifically with the extracellular domain of IGF-1R, was obtained using flow cytometry (FCM) screening using MCF-7 cells. Using immunostaining, MAb 1B1 detected IGF-1R mainly on the plasma membrane of MCF-7 cells. MAb 1B1 would be useful in FCM and immunofluorescence assays to detect IGF-1R-expressing cells for basic and clinical research.
authors.affiliation: 1 Department of Bioengineering, Graduate School of Engineering, Osaka City University , Sumiyoshi-ku, Osaka, Japan .
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Nakadate
authors.forename: Saori
authors.initials: S
authors.lastname: Yoshimura
authors.forename: Naoko
authors.initials: N
authors.lastname: Nakatani
authors.forename: Masayuki
authors.initials: M
authors.lastname: Azuma
authors.forename: Taro
authors.initials: T
authors.lastname: Tachibana
mesh_terms: D000818:Animals; D058846:Antibodies, Monoclonal, Murine-Derived / Q000096:biosynthesis / Q000737:chemistry*; D005260:Female; D005434:Flow Cytometry; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D006825:Hybridomas; D061986:MCF-7 Cells; D008810:Mice, Inbred C57BL; D017434:Protein Structure, Tertiary; D017526:Receptor, IGF Type 1 / Q000276:immunology*
chemical_list: D058846:Antibodies, Monoclonal, Murine-Derived; D017526:Receptor, IGF Type 1