title: DNA damage by histone radicals in nucleosome core particles.
abstract: Although DNA binding proteins shield the genetic material from diffusible reactive oxygen species by reacting with them, the resulting protein (peroxyl) radicals can oxidize the bound DNA. To explore this possible DNA damage by protein radicals, histone H4 proteins containing an azoalkane radical precursor at defined sites were prepared. Photolysis of a nucleosome core particle containing the modified protein produces DNA damage that is consistent with selective C4'-oxidation. The nucleotide(s) damaged is highly dependent on proximity to the protein radical. These experiments provide insight into the effects of oxidative stress on protein-bound DNA, revealing an additional layer of complexity concerning nucleic acid damage.
authors.affiliation: Department of Chemistry, Johns Hopkins University , 3400 North Charles Street, Baltimore, Maryland 21218, United States.
authors.forename: Chuanzheng
authors.initials: C
authors.lastname: Zhou
authors.forename: Marc M
authors.initials: MM
authors.lastname: Greenberg
mesh_terms: D004247:DNA / Q000187:drug effects*; D004249:DNA Damage; D006657:Histones / Q000737:chemistry / Q000494:pharmacology*; D009707:Nucleosomes / Q000187:drug effects*
chemical_list: D006657:Histones; D009707:Nucleosomes; D004247:DNA
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