title: One single amino Acid for estimation the content of total free amino acids in qingkailing injection using high-performance liquid chromatography-diode array detection.
abstract: Qingkailing injection (QKLI), a modern traditional Chinese medicine preparation, has been widely used in clinics due to its fast and significant efficacy in treatment of high fever. The free amino acids (AAs) were considered to be the most abundant active ingredients indisputably. So developing an accurate and simple determination method to measure the contents of total free AAs in QKLI is very crucial. In current study, the accurate and simple method of using one single standard AA for simultaneous quantification of multiple AAs (One for M) in QKLI was developed. Particularly, the calculation methods and the robustness of relative correction factors (RCFs) were investigated systematically. No statistically significant difference between these two quantification methods of One for M and classic regression equation was found by the t-test (P  =  95%, P > 0.05). The results showed that the precision (RSD < 4.88%), the robustness (RSD < 4.04%), and the average recoveries (94.11%-107.94%) of this newly proposed method all met the requirements for content determination. This One for M method will provide a scientific reference for the quantitative determination of AAs in other traditional Chinese medicines and their preparations owing to its accuracy and simplicity.
authors.affiliation: Science Experiment Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, No. 11 North Third Ring Road, Chaoyang District, Beijing 100029, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Science Experiment Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, No. 11 North Third Ring Road, Chaoyang District, Beijing 100029, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Wang
authors.affiliation: Science Experiment Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, No. 11 North Third Ring Road, Chaoyang District, Beijing 100029, China.
authors.forename: Jiankun
authors.initials: J
authors.lastname: Su
authors.affiliation: Science Experiment Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, No. 11 North Third Ring Road, Chaoyang District, Beijing 100029, China.
authors.forename: Haiyu
authors.initials: H
authors.lastname: Liu
authors.affiliation: Science Experiment Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, No. 11 North Third Ring Road, Chaoyang District, Beijing 100029, China.
authors.forename: Zhixin
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Science Experiment Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, No. 11 North Third Ring Road, Chaoyang District, Beijing 100029, China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Qin
authors.affiliation: Science Experiment Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, No. 11 North Third Ring Road, Chaoyang District, Beijing 100029, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: He
authors.affiliation: Science Experiment Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, No. 11 North Third Ring Road, Chaoyang District, Beijing 100029, China.
authors.forename: Long
authors.initials: L
authors.lastname: Peng
authors.affiliation: Science Experiment Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, No. 11 North Third Ring Road, Chaoyang District, Beijing 100029, China.
authors.forename: Mingxing
authors.initials: M
authors.lastname: Guo
authors.affiliation: Science Experiment Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, No. 11 North Third Ring Road, Chaoyang District, Beijing 100029, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Gao
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