title: Differences in saccharin preference and genetic alterations of the Tas1r3 gene among senescence-accelerated mouse strains and their parental AKR/J strain.
abstract: The senescence-accelerated mouse (SAM) is used as an animal model of senescence acceleration and age-associated disorders. SAM is derived from unexpected crosses between the AKR/J and unknown mouse strains. There are nine senescence-prone (SAMP) strains and three senescence-resistant (SAMR) strains. Although SAMP strains exhibit strain-specific and age-related pathological changes, the genes responsible for the pathologic changes in SAMP strains have not been comprehensively identified. In the present study, we evaluated sweet taste perception using the two-bottle test. We compared genotypes of the taste related gene, Tas1r3, using SAM strains and the parental AKR/J strain. The two-bottle test revealed that SAMR1 (R1), SAMP6 (P6), SAMP8 (P8), and SAMP10 (P10) mice were saccharin-preferring strains, whereas AKR/J did not prefer saccharin. All genotypes of the R1, P6, P8, and P10 strains at the polymorphic sites in Tas1r3, which is known to influence saccharin preference, were identical to those of C57BL6/J, a well-known saccharin-preferring strain, and were completely different from those of the parental AKR/J strain. These genetic alterations in SAM strains appear to arise from an unknown strain that is thought to have been crossed with AKR/J initially.
authors.affiliation: Support Unit for Animal Resources Development, Research Resources Center, RIKEN Brain Science Institute, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan. Electronic address: kimie@brain.riken.jp.
authors.forename: Kimie
authors.initials: K
authors.lastname: Niimi
authors.affiliation: Support Unit for Animal Resources Development, Research Resources Center, RIKEN Brain Science Institute, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
authors.forename: Eiki
authors.initials: E
authors.lastname: Takahashi
mesh_terms: D000818:Animals; D005518:Food Preferences / Q000502:physiology*; D005838:Genotype; D008806:Mice, Inbred AKR / Q000235:genetics; D008810:Mice, Inbred C57BL / Q000235:genetics; D008815:Mice, Inbred Strains / Q000235:genetics*; D011110:Polymorphism, Genetic; D043562:Receptors, G-Protein-Coupled / Q000235:genetics*; D012439:Saccharin / Q000008:administration & dosage*; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D013649:Taste / Q000235:genetics; D055697:Taste Perception / Q000235:genetics
chemical_list: D043562:Receptors, G-Protein-Coupled; C431926:taste receptors, type 1; D012439:Saccharin
keywords: Polymorphism; SAM; Saccharin; Tas1r3; Two-bottle test