title: Complete genomic sequence of the coronavirus transmissible gastroenteritis virus SHXB isolated in China.
abstract: A strain of transmissible gastroenteritis virus (TGEV), SHXB, was isolated in Shanghai, China. The complete genome of strain SHXB was sequenced, and its sequence was compared those of other TGEV strains in the GenBank database. The comparison showed that there were no insertions or deletions in the 5' and 3'- non-translated regions, in the nonstructural genes ORF1, ORF3, and ORF7, or in the genes encoding the structural proteins envelope (E), membrane (M) and nucleoprotein (N). A phenomenon in common with other strains was that nucleotide (nt) 655 of the spike (S) gene was G, and a common change in nt 1753 of the S gene was a T-to-G mutation that caused a serine-to-alanine mutation at amino acid 585, which is in the region of the main major antigenic sites A and B of the TGEV S protein. A 6-nt deletion was also found at nt 1123-1128 in all Purdue strains except the strain Virulent Purdue. Phylogenetic analysis showed that TGEV SHXB was closely related to the Purdue strains and shared a common ancestor with the Miller strains as well as strain PRCV-ISU-1.
authors.affiliation: Veterinary college, Nanjing Agricultural University, Weigang 1, Nanjing, 210095, Jiangsu, People's Republic of China.
authors.forename: Hu
authors.initials: H
authors.lastname: Weiwei
authors.forename: Yu
authors.initials: Y
authors.lastname: Qinghua
authors.forename: Zhu
authors.initials: Z
authors.lastname: Liqi
authors.forename: Liu
authors.initials: L
authors.lastname: Haofei
authors.forename: Zhao
authors.initials: Z
authors.lastname: Shanshan
authors.forename: Gao
authors.initials: G
authors.lastname: Qi
authors.forename: He
authors.initials: H
authors.lastname: Kongwang
authors.forename: Yang
authors.initials: Y
authors.lastname: Qian
mesh_terms: D000818:Animals; D002681:China; D016000:Cluster Analysis; D016679:Genome, Viral; D009154:Mutation; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D005760:Transmissible gastroenteritis virus / Q000235:genetics* / Q000302:isolation & purification; D014764:Viral Proteins / Q000235:genetics
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins
references.citation: Virology. 1995 Feb 1;206(2):817-22
references.pmid: 7856095
references.citation: J Virol. 1997 Apr;71(4):3285-7
references.pmid: 9060696
references.citation: JAMA. 2005 Nov 9;294(18):2291
references.pmid: 16278351
references.citation: Curr Top Microbiol Immunol. 2005;287:1-30
references.pmid: 15609507
references.citation: Cell. 1989 May 19;57(4):537-47
references.pmid: 2720781
references.citation: Virology. 1991 Jul;183(1):225-38
references.pmid: 1711257
references.citation: Adv Virus Res. 2006;66:193-292
references.pmid: 16877062
references.citation: Gene. 2008 Apr 15;412(1-2):12-25
references.pmid: 18295413
references.citation: J Am Vet Med Assoc. 1946 Apr;108:257-9
references.pmid: 21020443
references.citation: J Virol. 1994 Dec;68(12):8008-16
references.pmid: 7525985
references.citation: Arch Virol. 2000;145(6):1133-47
references.pmid: 10948987
references.citation: Rev Sci Tech. 2004 Aug;23(2):643-60
references.pmid: 15702725
references.citation: J Virol. 1999 Sep;73(9):7607-18
references.pmid: 10438851
references.citation: Virus Res. 2008 Apr;133(1):45-62
references.pmid: 17416434
references.citation: J Virol. 2003 Apr;77(7):4357-69
references.pmid: 12634392
references.citation: J Gen Virol. 1990 Jun;71 ( Pt 6):1313-23
references.pmid: 1693663
references.citation: Vet Rec. 1981 Jan 10;108(2):41
references.pmid: 7233794
references.citation: Virology. 1992 Sep;190(1):92-105
references.pmid: 1326823
references.citation: Viruses. 2012 Jun;4(6):980-1010
references.pmid: 22816036
references.citation: PLoS One. 2008 Jan 30;3(1):e1494
references.pmid: 18231581
references.citation: J Virol. 1995 May;69(5):3176-84
references.pmid: 7707547
references.citation: Vet Rec. 1987 Mar 7;120(10):226-30
references.pmid: 3576922
references.citation: J Virol. 1994 Oct;68(10):6523-34
references.pmid: 8083990
references.citation: Virus Genes. 2011 Feb;42(1):37-45
references.pmid: 20976535
references.citation: J Am Vet Med Assoc. 2007 Jul 1;231(1):56-67
references.pmid: 17605665
references.citation: Cell Microbiol. 2006 Jul;8(7):1147-57
references.pmid: 16819967
references.citation: Virology. 2007 Feb 20;358(2):424-35
references.pmid: 17023013
references.citation: Arch Virol. 1999;144(4):763-70
references.pmid: 10365166
references.citation: Virus Genes. 2001;23(1):105-18
references.pmid: 11556396
references.citation: PLoS Pathog. 2011 Jun;7(6):e1002090
references.pmid: 21695242
references.citation: J Virol. 2007 Jan;81(2):548-57
references.pmid: 17108024
references.citation: Am J Vet Res. 1977 Mar;38(3):307-10
references.pmid: 192108
references.citation: Adv Exp Med Biol. 1997;412:317-21
references.pmid: 9192036
references.citation: J Gen Virol. 1991 Mar;72 ( Pt 3):579-87
references.pmid: 1848593
references.citation: PLoS Biol. 2005 Jun;3(6):e172
references.pmid: 15884978