title: Preparative isolation and purification of phlorotannins from Ecklonia cava using centrifugal partition chromatography by one-step.
abstract: Various bioactive phlorotannins of Ecklonia cava (e.g., dieckol, eckol, 6,6-bieckol, phloroglucinol, phloroeckol, and phlorofucofuroeckol-A) are reported. However, their isolation and purification are not easy. Centrifugal partition chromatography (CPC) can be used to efficiently purify the various bioactive-compounds efficiently from E. cava. Phlorotannins are successfully isolated from the ethyl acetate (EtOAc) fraction of E. cava by CPC with a two-phase solvent system comprising n-hexane:EtOAc:methanol:water (2:7:3:7, v/v) solution. The dieckol (fraction I, 40.2mg), phlorofucofuroeckol-A (fraction III, 31.1mg), and fraction II (34.1mg) with 2,7-phloroglucinol-6,6-bieckol and pyrogallol-phloroglucinol-6,6-bieckol are isolated from the crude extract (500 mg) by a one-step CPC system. The purities of the isolated dieckol and phlorofucofuroeckol-A are ⩾90% according to high performance liquid chromatography (HPLC) and electrospray ionization multi stage tandem mass spectrometry analyses. The purified 2,7-phloroglucinol-6,6-bieckol and pyrogallol-phloroglucinol-6,6-bieckol are collected from fraction II by recycle-HPLC. Thus, the CPC system is useful for easy and simple isolation of phlorotannins from E. cava.
authors.affiliation: Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea.
authors.forename: Ji-Hyeok
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea.
authors.forename: Ju-Young
authors.initials: JY
authors.lastname: Ko
authors.affiliation: Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea.
authors.forename: Jae-Young
authors.initials: JY
authors.lastname: Oh
authors.affiliation: Natural Product Research Center, Hanyang University, Daejeon-dong, Ahnsan, Gyeongi-do, Republic of Korea.
authors.forename: Chul-Young
authors.initials: CY
authors.lastname: Kim
authors.affiliation: Natural Product Research Center, KIST Gangneung Institute, Daejeon-dong, Gangneung, Gangwon-do, Republic of Korea.
authors.forename: Hee-Ju
authors.initials: HJ
authors.lastname: Lee
authors.affiliation: Department of Food Science and Technology, Pukyong National University, Busan 608-737, Republic of Korea. Electronic address: jikim@pknu.ac.kr.
authors.forename: Jaeil
authors.initials: J
authors.lastname: Kim
authors.affiliation: Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea. Electronic address: youjin@jejunu.ac.kr.
authors.forename: You-Jin
authors.initials: YJ
authors.lastname: Jeon
mesh_terms: D002845:Chromatography / Q000295:instrumentation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D000459:Phaeophyceae / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification*; D021241:Spectrometry, Mass, Electrospray Ionization; D014675:Vegetables / Q000737:chemistry*
chemical_list: D010936:Plant Extracts
keywords: Centrifugal partition chromatography (CPC); Ecklonia cava; Phlorotannin