title: The effect of alendronate-loaded polycarprolactone nanofibrous scaffolds on osteogenic differentiation of adipose-derived stem cells in bone tissue regeneration.
abstract: The osteogenic effect of culturing adipose-derived stem cells (ADSCs) on alendronate (Aln)-loaded polycarprolactone (PCL) nanofibrous scaffolds was evaluated by examining alkaline phosphatase (ALP) activity, calcium content, and expression of osteogenic differentiation genes in vitro. The 10% Aln/PCL nanofibrous scaffolds showed more ALP activity, mineralization, and osteocalcin and osteopontin mRNA than the 1% or 5% Aln/PCL nanofibrous scaffolds. The capacity of Aln/PCL nanofibrous scaffolds to regenerate new bone was studied in a rat calvarial defect model. New bone formation in vivo was evaluated by radiography, micro-computed tomography, and histological analysis. At 8 weeks after implantation, Aln/PCL scaffolds had a positive effect on bone regeneration and matrix formation. These results suggested that Aln/PCL nanofibrous scaffolds enhanced the osteogenic differentiation of ADSCs in vitro and bone formation in vivo.
authors.forename: Young-Pil
authors.initials: YP
authors.lastname: Yun
authors.forename: Seung-Ju
authors.initials: SJ
authors.lastname: Kim
authors.forename: Youn-Mook
authors.initials: YM
authors.lastname: Lim
authors.forename: Kyeongsoon
authors.initials: K
authors.lastname: Park
authors.forename: Hak-Jun
authors.initials: HJ
authors.lastname: Kim
authors.forename: Sung-In
authors.initials: SI
authors.lastname: Jeong
authors.forename: Sung Eun
authors.initials: SE
authors.lastname: Kim
authors.forename: Hae-Ryong
authors.initials: HR
authors.lastname: Song
mesh_terms: D017667:Adipocytes / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D019386:Alendronate / Q000008:administration & dosage* / Q000737:chemistry; D000818:Animals; D050071:Bone Density Conservation Agents / Q000008:administration & dosage; D001861:Bone Regeneration / Q000187:drug effects / Q000502:physiology; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D004058:Diffusion; D004867:Equipment Design; D019544:Equipment Failure Analysis; D008422:Materials Testing; D057139:Nanofibers / Q000008:administration & dosage / Q000737:chemistry*; D010012:Osteogenesis / Q000187:drug effects / Q000502:physiology*; D011091:Polyesters / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D013499:Surface Properties; D054457:Tissue Scaffolds
chemical_list: D050071:Bone Density Conservation Agents; D011091:Polyesters; C016240:polycaprolactone; D019386:Alendronate