title: O-carboxymethyl chitosan nanoparticles for metformin delivery to pancreatic cancer cells.
abstract: In this work we developed metformin loaded O-carboxymethyl chitosan (O-CMC) nanoparticles (NPs) by ionic-gelation method. The prepared NPs of 240 ± 50 nm size with spherical morphology exhibited a pH sensitive release of metformin in vitro. Cytotoxicity studies showed that the drug-incorporated NPs induced significant toxicity on pancreatic cancer cells (MiaPaCa-2) compared to normal cells (L929). Metformin loaded NPs exhibited nonspecific internalization by normal and pancreatic cancer cells; however metformin released from the NPs induced preferential toxicity on pancreatic cancer cells. Our preliminary studies suggested that such a novel approach could possibly overcome the current limitations of metformin in its clinical application against pancreatic cancer.
authors.affiliation: Amrita Centre for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences & Research Centre, Amrita Vishwa Vidyapeetham University, Kochi 682041, India.
authors.forename: K S
authors.initials: KS
authors.lastname: Snima
authors.affiliation: Amrita Centre for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences & Research Centre, Amrita Vishwa Vidyapeetham University, Kochi 682041, India.
authors.forename: R
authors.initials: R
authors.lastname: Jayakumar
authors.affiliation: Department of Endocrinology, Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham University, Kochi 682041, India.
authors.forename: A G
authors.initials: AG
authors.lastname: Unnikrishnan
authors.affiliation: Amrita Centre for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences & Research Centre, Amrita Vishwa Vidyapeetham University, Kochi 682041, India.
authors.forename: Shantikumar V
authors.initials: SV
authors.lastname: Nair
authors.affiliation: Amrita Centre for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences & Research Centre, Amrita Vishwa Vidyapeetham University, Kochi 682041, India.
authors.forename: Vinoth-Kumar
authors.initials: VK
authors.lastname: Lakshmanan
mesh_terms: D045744:Cell Line, Tumor; D048271:Chitosan / Q000031:analogs & derivatives* / Q000737:chemistry; D004337:Drug Carriers; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D008687:Metformin / Q000008:administration & dosage* / Q000493:pharmacokinetics / Q000494:pharmacology; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles / Q000737:chemistry*; D010190:Pancreatic Neoplasms / Q000188:drug therapy* / Q000473:pathology
chemical_list: D004337:Drug Carriers; C504164:O-carboxymethylchitosan; D048271:Chitosan; D008687:Metformin