title: Interepithelial signaling with nephric duct is required for the formation of overlying coelomic epithelial cell sheet.
abstract: In most organs of the body, epithelial tissues are supported by their own basement membrane and underlying stroma, the latter being regarded as a complex of amorphous cells, extracellular matrices, and soluble factors. We demonstrate here that an epithelial tube can serve as a component of stroma that supports the formation of epithelial cell sheet derived from a different origin. During development of the mesonephros in chicken embryos, the intermediate mesoderm (IMM), which contains the Wolffian duct (WD) and its associated tubules, is overlain by a sheet of epithelial cells derived from lateral plate (coelomic) mesoderm. We describe that in normal embryos, epitheliogenesis of IMM tubes and the adjacent coelomic cell sheet proceed in a coordinated manner. When the WD was surgically ablated, the overlying coelomic epithelium exhibited aberrant morphology accompanied by a punctated basement membrane. Furthermore, the WD-ablated coelomic epithelium became susceptible to latent external stress; electroporation of Rac1 resulted in epithelial-to-mesenchymal transitions (EMTs) within the coelomic epithelium. The distorted coelomic epithelium was rescued by implanting fibronectin-producing cells in place of the WD, suggesting that fibronectin provided by WD has an important role acting interepithelially. This notion was corroborated further by directly visualizing a translocation of EGFP-tagged fibronectin from fibronectin-producing to -receiving epithelia in vivo. Our findings provide a novel insight into interepithelial signaling that also might occur in adult tissues to protect against EMT and suggest a possible new target for anticancer therapeutic strategy.
authors.affiliation: Department of Zoology, Division of Biological Science, Graduate School of Science, Kyoto University, Kitashirakawa, Sakyo-ku, Kyoto 606-8502, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Yoshino
authors.forename: Daisuke
authors.initials: D
authors.lastname: Saito
authors.forename: Yuji
authors.initials: Y
authors.lastname: Atsuta
authors.forename: Chihiro
authors.initials: C
authors.lastname: Uchiyama
authors.forename: Shinya
authors.initials: S
authors.lastname: Ueda
authors.forename: Kiyotoshi
authors.initials: K
authors.lastname: Sekiguchi
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Takahashi
mesh_terms: D000818:Animals; D002642:Chick Embryo; D047108:Embryonic Development / Q000502:physiology; D058750:Epithelial-Mesenchymal Transition / Q000502:physiology; D004848:Epithelium / Q000196:embryology; D005109:Extracellular Matrix / Q000502:physiology; D005353:Fibronectins / Q000235:genetics / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D039482:Integrin alpha5 / Q000378:metabolism; D019012:Integrin beta1 / Q000378:metabolism; D007668:Kidney / Q000002:abnormalities / Q000196:embryology*; D008648:Mesoderm / Q000196:embryology; D038081:Organogenesis / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D014928:Wolffian Ducts / Q000002:abnormalities / Q000196:embryology*
chemical_list: D005353:Fibronectins; D039482:Integrin alpha5; D019012:Integrin beta1; D012333:RNA, Messenger
keywords: epithelialization; extracellular matrix; kidney development; metastasis; organogenesis
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