title: Tumor-activated TCRγδ⁺ T cells from gastric cancer patients induce the antitumor immune response of TCRαβ⁺ T cells via their antigen-presenting cell-like effects.
abstract: Human γδ T cells display the principal characteristics of professional antigen-presenting cells (APCs), in addition to playing a vital role in immunity through cytokine secretion and their cytotoxic activity. However, it is not clear whether γδ T cells perform APC-like functions under pathological conditions. In this study, we showed that, in contrast to peripheral-derived γδ T cells directly isolated from PBMCs of gastric cancer patients, tumor-activated γδ T cells not only killed tumor cells efficiently but also strongly induced primary CD4(+) and CD8(+) αβ T cells proliferation and differentiation. More importantly, they abrogated the immunosuppression induced by CD4(+)CD25(+) Treg cells and induced the cytotoxic function of CD8(+) αβ T cells from patients with gastric cancer. In conclusion, tumor-activated γδ T cells can induce adaptive immune responses through their APC-like functions, and these cells may be a potentially useful tool in the development of tumor vaccines and immunotherapy.
authors.affiliation: Department of Nuclear Medicine, The Affiliated Hospital of Jiangsu University, 438 Jiefang Road, Zhenjiang 212001, China ; Institute of Oncology, The Affiliated Hospital to Jiangsu University, 438 Jiefang Road, Zhenjiang 212001, China.
authors.forename: Chaoming
authors.initials: C
authors.lastname: Mao
authors.affiliation: Department of Nuclear Medicine, The Affiliated Hospital of Jiangsu University, 438 Jiefang Road, Zhenjiang 212001, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Mou
authors.affiliation: Department of Nuclear Medicine, The Affiliated Hospital of Jiangsu University, 438 Jiefang Road, Zhenjiang 212001, China ; Institute of Oncology, The Affiliated Hospital to Jiangsu University, 438 Jiefang Road, Zhenjiang 212001, China.
authors.forename: Yuepeng
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Nuclear Medicine, The Affiliated Hospital of Jiangsu University, 438 Jiefang Road, Zhenjiang 212001, China.
authors.forename: Guoyue
authors.initials: G
authors.lastname: Yuan
authors.affiliation: Department of Nuclear Medicine, The Affiliated Hospital of Jiangsu University, 438 Jiefang Road, Zhenjiang 212001, China.
authors.forename: Chengcheng
authors.initials: C
authors.lastname: Xu
authors.affiliation: Department of Nuclear Medicine, The Affiliated Hospital of Jiangsu University, 438 Jiefang Road, Zhenjiang 212001, China.
authors.forename: Hongli
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Nuclear Medicine, The Affiliated Hospital of Jiangsu University, 438 Jiefang Road, Zhenjiang 212001, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Zheng
authors.affiliation: Department of Immunology and Laboratory Immunology, School of Medical Science and Laboratory Medicine, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Tong
authors.affiliation: Department of Immunology and Laboratory Immunology, School of Medical Science and Laboratory Medicine, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, China.
authors.forename: Shengjun
authors.identifier: 0000-0001-6584-1183
authors.initials: S
authors.lastname: Wang
authors.affiliation: Institute of Oncology, The Affiliated Hospital to Jiangsu University, 438 Jiefang Road, Zhenjiang 212001, China.
authors.forename: Deyu
authors.initials: D
authors.lastname: Chen
mesh_terms: D000328:Adult; D000368:Aged; D017951:Antigen Presentation / Q000276:immunology; D000938:Antigen-Presenting Cells / Q000276:immunology; D016022:Case-Control Studies; D016207:Cytokines / Q000378:metabolism; D003602:Cytotoxicity, Immunologic; D005260:Female; D006801:Humans; D056747:Immunomodulation; D008213:Lymphocyte Activation / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000378:metabolism*; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000378:metabolism*; D013274:Stomach Neoplasms / Q000276:immunology* / Q000378:metabolism*; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism*
chemical_list: D016207:Cytokines; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta
references.citation: J Exp Med. 2003 Jan 20;197(2):163-8
references.pmid: 12538656
references.citation: Immunity. 2007 Aug;27(2):334-48
references.pmid: 17656116
references.citation: Cancer Res. 2007 Jan 1;67(1):5-8
references.pmid: 17210676
references.citation: J Immunol. 2012 Feb 15;188(4):1708-16
references.pmid: 22250090
references.citation: Proc Natl Acad Sci U S A. 2010 May 11;107(19):8730-5
references.pmid: 20413723
references.citation: Cancer Immunol Immunother. 2013 May;62(5):863-73
references.pmid: 23359088
references.citation: J Immunol. 2012 Nov 15;189(10):5029-36
references.pmid: 23034170
references.citation: J Immunotoxicol. 2012 Jul-Sep;9(3):259-66
references.pmid: 22632132
references.citation: Cancer Res. 2007 Aug 1;67(15):7450-7
references.pmid: 17671215
references.citation: Science. 2005 Jul 8;309(5732):264-8
references.pmid: 15933162
references.citation: Scand J Immunol. 2003 Aug;58(2):211-20
references.pmid: 12869143
references.citation: Immunol Lett. 2007 May 15;110(1):42-53
references.pmid: 17451812
references.citation: Scand J Immunol. 2008 May;67(5):441-7
references.pmid: 18312485
references.citation: J Immunol. 2005 Oct 15;175(8):5481-8
references.pmid: 16210656
references.citation: Blood. 2008 Jun 15;111(12):5629-36
references.pmid: 18388182
references.citation: J Immunol. 2013 Jun 15;190(12):6673-80
references.pmid: 23686489
references.citation: Immunity. 2001 Feb;14(2):123-33
references.pmid: 11239445
references.citation: Cell Mol Life Sci. 2011 Jul;68(14):2345-55
references.pmid: 21541698
references.citation: Onkologie. 2008 May;31(5):243-8
references.pmid: 18497513
references.citation: J Exp Med. 2004 Apr 5;199(7):1005-10
references.pmid: 15051759
references.citation: J Hepatol. 2013 May;58(5):977-83
references.pmid: 23262246
references.citation: Proc Natl Acad Sci U S A. 2009 Feb 17;106(7):2307-12
references.pmid: 19171897
references.citation: Immunol Rev. 2007 Feb;215:59-76
references.pmid: 17291279
references.citation: Nat Rev Immunol. 2009 Oct;9(10):704-16
references.pmid: 19859065
references.citation: Science. 2005 Jul 8;309(5732):252-3
references.pmid: 16002604
references.citation: Cancer Res. 2002 Nov 1;62(21):6178-86
references.pmid: 12414645
references.citation: Trends Immunol. 2002 Jan;23(1):14-8
references.pmid: 11801449
references.citation: Trends Immunol. 2012 May;33(5):199-206
references.pmid: 22364810
references.citation: Eur J Cardiothorac Surg. 2010 May;37(5):1191-7
references.pmid: 20137969
references.citation: Cancer Res. 2009 Nov 15;69(22):8710-7
references.pmid: 19887600
references.citation: Science. 2001 Oct 19;294(5542):605-9
references.pmid: 11567106
references.citation: Nat Rev Immunol. 2013 Feb;13(2):88-100
references.pmid: 23348415
references.citation: J Immunol. 2008 May 1;180(9):6044-53
references.pmid: 18424725
references.citation: J Exp Med. 2004 Mar 15;199(6):879-84
references.pmid: 15007091
references.citation: J Immunol. 2009 Jun 1;182(11):7287-96
references.pmid: 19454726
references.citation: Cancer Res. 2010 Dec 15;70(24):10024-7
references.pmid: 21159627
references.citation: Cancer Immunol Immunother. 2009 May;58(5):641-52
references.pmid: 18791713
references.citation: Cancer Lett. 2010 Nov 1;297(1):126-36
references.pmid: 20537791