title: Fluorescence plate reader for quantum dot-protein bioconjugation analysis.
abstract: We present here a new and alternative method that uses a Fluorescence Plate Reader in a different approach, not to study protein-protein interactions, but to evaluate the efficiency of the protein bioconjugation to quantum dots (QDs). The method is based on the QDs' native fluorescence and was successfully tested by employing two different QDs-proteins conjugation methodologies, one by promoting covalent binding and other by inducing adsorption processes. For testing, we used bioconjugates between carboxyl coated CdTe QDs and bovine serum albumin, concanavalin A lectin and anti-A antibody. Flow cytometry and fluorescence spectroscopy studies corroborated the results found by the Fluorescence Plate Reader assay. This kind of analysis is important because poor bioconjugation efficiency leads to unsuccessful applications of the fluorescent bioconjugates. We believe that our method presents the possibility of performing semi-quantitative and simultaneous analysis of different samples with accuracy taking the advantage of the high sensitivity of optical based measurements.
authors.forename: Kilmara H G
authors.initials: KH
authors.lastname: Carvalho
authors.forename: Aluizio G
authors.initials: AG
authors.lastname: Brasil
authors.forename: Paulo E
authors.initials: PE
authors.lastname: Cabral Filho
authors.forename: Denise P L A
authors.initials: DP
authors.lastname: Tenório
authors.forename: Ana C A
authors.initials: AC
authors.lastname: de Siqueira
authors.forename: Elisa S
authors.initials: ES
authors.lastname: Leite
authors.forename: Adriana
authors.initials: A
authors.lastname: Fontes
authors.forename: Beate S
authors.initials: BS
authors.lastname: Santos
mesh_terms: D019187:Cadmium Compounds / Q000737:chemistry; D005434:Flow Cytometry; D045663:Quantum Dots; D012709:Serum Albumin / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013691:Tellurium / Q000737:chemistry
chemical_list: D019187:Cadmium Compounds; D012709:Serum Albumin; D013691:Tellurium; C028337:cadmium telluride