title: An outbreak of aseptic meningitis caused by a distinct lineage of coxsackievirus B5 in China.
abstract: In 2009, an outbreak of aseptic meningitis caused by coxsackievirus B5 (CVB5) occurred in China. Epidemiological investigations of this outbreak revealed that the proportion of severe cases (14/43, 33%) was higher than in other outbreaks associated with CVB5 in China. Phylogenetic analysis of the entire VP1 sequences demonstrated that the CVB5 isolates from the severe cases form a distinct lineage belonging to genogroup E with the Shandong isolates of 2009. A substitution of serine (S) to asparagine (N) at amino acid 95 in the VP1 region may be a major virulence determinant for the virus. Our findings suggest that this new lineage of CVB5 is circulating in China. Further genetic studies are needed in order to gain a better insight into the genetic variability of CVB5 isolates and the relationship with pathogenicity.
authors.affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, 20 Dongda Street, Fengtai District, Beijing 100071, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Liu
authors.affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, 20 Dongda Street, Fengtai District, Beijing 100071, China.
authors.forename: Leili
authors.initials: L
authors.lastname: Jia
authors.affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, 20 Dongda Street, Fengtai District, Beijing 100071, China.
authors.forename: Jiye
authors.initials: J
authors.lastname: Yin
authors.affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, 20 Dongda Street, Fengtai District, Beijing 100071, China.
authors.forename: Zhihao
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, 20 Dongda Street, Fengtai District, Beijing 100071, China.
authors.forename: Zhongqiang
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, 20 Dongda Street, Fengtai District, Beijing 100071, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Li
authors.affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, 20 Dongda Street, Fengtai District, Beijing 100071, China.
authors.forename: Rongzhang
authors.initials: R
authors.lastname: Hao
authors.affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, 20 Dongda Street, Fengtai District, Beijing 100071, China.
authors.forename: Ligui
authors.initials: L
authors.lastname: Wang
authors.affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, 20 Dongda Street, Fengtai District, Beijing 100071, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, 20 Dongda Street, Fengtai District, Beijing 100071, China. Electronic address: qiushf0613@hotmail.com.
authors.forename: Shaofu
authors.initials: S
authors.lastname: Qiu
authors.affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, 20 Dongda Street, Fengtai District, Beijing 100071, China. Electronic address: hongbinsong@263.net.
authors.forename: Hongbin
authors.initials: H
authors.lastname: Song
mesh_terms: D000595:Amino Acid Sequence; D002648:Child; D002675:Child, Preschool; D002681:China / Q000453:epidemiology; D004196:Disease Outbreaks; D029822:Enterovirus B, Human / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D008297:Male; D008582:Meningitis, Aseptic / Q000453:epidemiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000302:isolation & purification; D017422:Sequence Analysis, DNA
chemical_list: D012367:RNA, Viral
keywords: Aseptic meningitis; Coxsackievirus B5; Genetically distinct; Outbreak; Substitution