title: Mapping antiretroviral drugs in tissue by IR-MALDESI MSI coupled to the Q Exactive and comparison with LC-MS/MS SRM assay.
abstract: This work describes the coupling of the IR-MALDESI imaging source with the Q Exactive mass spectrometer. IR-MALDESI MSI was used to elucidate the spatial distribution of several HIV drugs in cervical tissues that had been incubated in either a low or high concentration. Serial sections of those analyzed by IR-MALDESI MSI were homogenized and analyzed by LC-MS/MS to quantify the amount of each drug present in the tissue. By comparing the two techniques, an agreement between the average intensities from the imaging experiment and the absolute quantities for each drug was observed. This correlation between these two techniques serves as a prerequisite to quantitative IR-MALDESI MSI. In addition, a targeted MS(2) imaging experiment was also conducted to demonstrate the capabilities of the Q Exactive and to highlight the added selectivity that can be obtained with SRM or MRM imaging experiments.
authors.affiliation: W. M. Keck FT Mass Spectrometry Laboratory, Department of Chemistry, North Carolina State University, Raleigh, NC, 27695, USA.
authors.forename: Jeremy A
authors.initials: JA
authors.lastname: Barry
authors.forename: Guillaume
authors.initials: G
authors.lastname: Robichaud
authors.forename: Mark T
authors.initials: MT
authors.lastname: Bokhart
authors.forename: Corbin
authors.initials: C
authors.lastname: Thompson
authors.forename: Craig
authors.initials: C
authors.lastname: Sykes
authors.forename: Angela D M
authors.initials: AD
authors.lastname: Kashuba
authors.forename: David C
authors.initials: DC
authors.lastname: Muddiman
mesh_terms: D000225:Adenine / Q000031:analogs & derivatives / Q000032:analysis / Q000737:chemistry / Q000493:pharmacokinetics; D044966:Anti-Retroviral Agents / Q000032:analysis* / Q000737:chemistry / Q000493:pharmacokinetics*; D002584:Cervix Uteri / Q000737:chemistry / Q000378:metabolism; D002853:Chromatography, Liquid / Q000379:methods*; D003841:Deoxycytidine / Q000031:analogs & derivatives / Q000032:analysis / Q000737:chemistry / Q000493:pharmacokinetics; D000068679:Emtricitabine; D005260:Female; D006651:Histocytochemistry; D006801:Humans; D057054:Molecular Imaging / Q000379:methods*; D063065:Organophosphonates / Q000032:analysis / Q000737:chemistry / Q000493:pharmacokinetics; D011760:Pyrrolidinones / Q000032:analysis / Q000737:chemistry / Q000493:pharmacokinetics; D000068898:Raltegravir Potassium; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D000068698:Tenofovir; D014018:Tissue Distribution
chemical_list: D044966:Anti-Retroviral Agents; D063065:Organophosphonates; D011760:Pyrrolidinones; D003841:Deoxycytidine; D000068898:Raltegravir Potassium; D000068698:Tenofovir; D000068679:Emtricitabine; D000225:Adenine
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