title: In vivo wound healing performance of drug loaded electrospun composite nanofibers transdermal patch.
abstract: Acute injuries or wound is required the fast delivery of drug to control infections without any side effect. In this direction in the present investigation, antibiotic ciprofloxacin loaded hydrophilic biodegradable poly vinyl alcohol (PVA) and sodium alginate (NaAlg) electrospun composite nanofiber based transdermal patch was developed for local delivery of antibiotic drug. The antibiotic drug ciprofloxacin was loaded in it by active loading. The drug entrapped in the composite nanofibers was confirmed by the scanning electron microscopy and swelling behavior. The in vivo studies were carried on male rabbits by using the drug loaded and unloaded composite nanofibers transdermal patch and marketed one. It is observed that, in vitro activity provides a sustained and controlled release pattern of the drug from transdermal patch. The mechanism of drug release was also studied using different models. The nanofiber transdermal patch follows the Higuchi and Korsmeyer-Peppas model for drug release. The in vivo studies demonstrate that, wound healing takes place in less time as compared drug unloaded patch. Hydroxyproline produced in wound bed with time shows that it content is maximum in case drug loaded PAV-NaAlg patch. This demonstrates that wound healing rate is higher in case drug loaded PVA-NaAlg transdermal patch.
authors.affiliation: Division of Materials Physics and Engineering, CSIR-National Physical Laboratory, New Delhi 110012, India; Nanomedicine Research Centre, Indo-Soviet Friendship College of Pharmacy, Moga, Punjab, India.
authors.forename: K
authors.initials: K
authors.lastname: Kataria
authors.affiliation: Division of Materials Physics and Engineering, CSIR-National Physical Laboratory, New Delhi 110012, India.
authors.forename: A
authors.initials: A
authors.lastname: Gupta
authors.affiliation: Nanomedicine Research Centre, Indo-Soviet Friendship College of Pharmacy, Moga, Punjab, India.
authors.forename: G
authors.initials: G
authors.lastname: Rath
authors.affiliation: Division of Materials Physics and Engineering, CSIR-National Physical Laboratory, New Delhi 110012, India.
authors.forename: R B
authors.initials: RB
authors.lastname: Mathur
authors.affiliation: Division of Materials Physics and Engineering, CSIR-National Physical Laboratory, New Delhi 110012, India. Electronic address: dhakate@mail.nplindia.org.
authors.forename: S R
authors.initials: SR
authors.lastname: Dhakate
mesh_terms: D000279:Administration, Cutaneous; D000464:Alginates / Q000737:chemistry*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000737:chemistry; D015415:Biomarkers / Q000378:metabolism; D002626:Chemistry, Pharmaceutical; D002939:Ciprofloxacin / Q000008:administration & dosage* / Q000737:chemistry; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006909:Hydroxyproline / Q000378:metabolism; D007700:Kinetics; D008297:Male; D008855:Microscopy, Electron, Scanning; D008956:Models, Chemical; D057139:Nanofibers; D036103:Nanotechnology; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D011817:Rabbits; D012867:Skin / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D012995:Solubility; D013499:Surface Properties; D013678:Technology, Pharmaceutical / Q000379:methods; D057968:Transdermal Patch; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000464:Alginates; D000900:Anti-Bacterial Agents; D015415:Biomarkers; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D006603:Hexuronic Acids; D002939:Ciprofloxacin; D020723:Glucuronic Acid; D011142:Polyvinyl Alcohol; D006909:Hydroxyproline
keywords: Drug release; Electrospinning; Hydroxyproline; In vivo; Nanofibers; Transdermal patch