title: Interaction of arabinogalactan with mucins.
abstract: Arabinogalactan is a naturally-occurring, densely branched, polysaccharide mainly made-up of galactose and arabinose with variable amounts of uronic acids, which received attention for several industrial and biomedical applications. The ability of Western Larch arabinogalactan to interact with mucins was assessed by both classical gel filtration chromatography and frontal chromatography on Sephacryl S300 resin. The shift of arabinogalactan elution volume in classical gel filtration chromatography induced by both bovine submaxillary mucin and porcine gastric mucin resulted useful for revealing the occurrence of an interaction between arabinogalactan and mucins. A frontal gel chromatography, in which arabinogalactan is used as eluent, enabled a dissociation constant of 5×10(-6)M to be measured for the arabinogalactan-bovine submaxillary mucin complex, with approximately 50 equivalents of arabinogalactan bound per mucin mole. The mucoadhesivity of arabinogalactan may be a relevant feature for its biomedical and industrial applications.
authors.affiliation: Department of Biology, University of Pisa, via S. Zeno, 51, Pisa 56127, Italy.
authors.forename: Roberta
authors.initials: R
authors.lastname: Moschini
authors.affiliation: Department of Biology, University of Pisa, via S. Zeno, 51, Pisa 56127, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Gini
authors.affiliation: Department of Biology, University of Pisa, via S. Zeno, 51, Pisa 56127, Italy.
authors.forename: Mario
authors.initials: M
authors.lastname: Cappiello
authors.affiliation: Department of Biology, University of Pisa, via S. Zeno, 51, Pisa 56127, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Balestri
authors.affiliation: Opocrin SpA, via Pacinotti, 3, Corlo di Formigine, Modena 41010, Italy.
authors.forename: Giulia
authors.initials: G
authors.lastname: Falcone
authors.affiliation: Opocrin SpA, via Pacinotti, 3, Corlo di Formigine, Modena 41010, Italy.
authors.forename: Enrico
authors.initials: E
authors.lastname: Boldrini
authors.affiliation: Department of Biology, University of Pisa, via S. Zeno, 51, Pisa 56127, Italy.
authors.forename: Umberto
authors.initials: U
authors.lastname: Mura
authors.affiliation: Department of Biology, University of Pisa, via S. Zeno, 51, Pisa 56127, Italy. Electronic address: adelcorso@biologia.unipi.it.
authors.forename: Antonella
authors.initials: A
authors.lastname: Del-Corso
mesh_terms: D000818:Animals; D001089:Arabinose / Q000737:chemistry; D002417:Cattle; D002850:Chromatography, Gel; D005685:Galactans / Q000737:chemistry*; D005690:Galactose / Q000737:chemistry; D028221:Larix / Q000737:chemistry; D009077:Mucins / Q000737:chemistry / Q000378:metabolism*; D010936:Plant Extracts / Q000737:chemistry; D011485:Protein Binding; D014574:Uronic Acids / Q000737:chemistry*
chemical_list: D005685:Galactans; D009077:Mucins; D010936:Plant Extracts; D014574:Uronic Acids; D001089:Arabinose; C005653:arabinogalactan; D005690:Galactose
keywords: Arabinogalactan; Mucin; Mucoadesivity