title: Response of bacterial community structure to seasonal fluctuation and anthropogenic pollution on coastal water of Alang-Sosiya ship breaking yard, Bhavnagar, India.
abstract: Bacterial community structure was analyzed from coastal water of Alang-Sosiya ship breaking yard (ASSBY), world's largest ship breaking yard, near Bhavnagar, using 16S rRNA gene sequencing (cultured dependent and culture independent). In clone libraries, total 2324 clones were retrieved from seven samples (coastal water of ASSBY for three seasons along with one pristine coastal water) which were grouped in 525 operational taxonomic units. Proteobacteria was found to be dominant in all samples. In pristine samples, Gammaproteobacteria was found to be dominant, whereas in polluted samples dominancy of Gammaproteobacteria has shifted to Betaproteobacteria and Epsilonproteobacteria. Richness and diversity indices also indicated that bacterial community in pristine sample was the most diverse followed by summer, monsoon and winter samples. To the best of knowledge, this is the first study describing bacterial community structure from coastal water of ASSBY, and it suggests that seasonal fluctuation and anthropogenic pollutions alters the bacterial community structure.
authors.affiliation: Environmental Genomics and Proteomics Lab, BRD School of Biosciences, Vadtal Road, Satellite Campus, Post Box No. 39, Sardar Patel University, Vallabh Vidyanagar 388 120, Gujarat, India. Electronic address: patelva1986@gmail.com.
authors.forename: Vilas
authors.initials: V
authors.lastname: Patel
authors.affiliation: Molecular Biology Unit, National Center for Cell Science, Ganeshkhind, Pune 411007, Maharashtra, India.
authors.forename: Hitendra
authors.initials: H
authors.lastname: Munot
authors.affiliation: Molecular Biology Unit, National Center for Cell Science, Ganeshkhind, Pune 411007, Maharashtra, India.
authors.forename: Yogesh S
authors.initials: YS
authors.lastname: Shouche
authors.affiliation: Environmental Genomics and Proteomics Lab, BRD School of Biosciences, Vadtal Road, Satellite Campus, Post Box No. 39, Sardar Patel University, Vallabh Vidyanagar 388 120, Gujarat, India. Electronic address: datta_madamwar@yahoo.com.
authors.forename: Datta
authors.initials: D
authors.lastname: Madamwar
mesh_terms: D007194:India; D059013:Microbial Consortia; D012336:RNA, Ribosomal, 16S / Q000737:chemistry*; D012621:Seasons; D012623:Seawater / Q000382:microbiology*; D014871:Water Microbiology; D014876:Water Pollution
chemical_list: D012336:RNA, Ribosomal, 16S
keywords: 16S r-RNA gene; Alang–Sosiya ship breaking yard; Bacterial community structure; Betaproteobacteria; Seasonal variation