title: [The effect and mechanism of curcumin derivative B06 on the myocardium from type 2 diabetic rats].
abstract: OBJECTIVE
  To investigate the protective effect and mechanism of curcumin derivatives B06 on myocardium from type 2 diabetic rats.
  
  
  METHODS
  Thirty-five male SD rats were randomly divided into 5 groups, normal control group (NC group), high fat group (HF group), high fat treatment group (FT group), diabetes mellitus group (DM group) and diabetes treatment group (DT group) (n = 7). The late four groups were fed with high fat food, after four weeks of high fat feeding, the rats from DM group and DT group were injected with low dosage of streptozocin intraperitoneally to induce diabetes mellitus, FT group and DT group were gavaged with curcumin derivatives B06 at the dosage of 0.2 mg/kg x d. The blood glucose and lipid were detected biochemically, blood insulin was assayed by ELISA and the insulin resistance index was calculated, the morphology of myocardium was observed by light and transmission electron microscopy, the protein expression of AMP-activated protein kinase alpha (AMPKalpha) and phosphorylated AMP-activated protein kinase alpha (p-AMPKalpha) in myocardium were tested by Western blot.
  
  
  RESULTS
  The level of blood glucose, lipid, insulin and the insulin resistance index were increased in HF group and DM group, but they were decreased after the treatment with B06. The expression of AMPKalpha and p-AMPKalpha were decreased, but they became increased after the treatment of B06. There were increased collagen fibers in interstitium and expansion of mitochondria in cytoplasm of myocardium from DM group, but they were ameliorated in B06 treatment group.
  
  
  CONCLUSIONS
  It is suggested that B06 may relieve the damage of myocardium from type 2 diabetic rats and the increased expression of AMPKalpha and p-AMPKalpha may be involved in it.
authors.forename: Zhong-Min
authors.initials: ZM
authors.lastname: Lin
authors.forename: Li-Zhuo
authors.initials: LZ
authors.lastname: Jiao
authors.forename: Yi
authors.initials: Y
authors.lastname: Zheng
authors.forename: Xiao-Ya
authors.initials: XY
authors.lastname: Wang
authors.forename: Ling
authors.initials: L
authors.lastname: Wang
authors.forename: Wang-Wang
authors.initials: WW
authors.lastname: Liu
authors.forename: Meng-Fei
authors.initials: MF
authors.lastname: Xu
authors.forename: Xiu-Huan
authors.initials: XH
authors.lastname: Ji
authors.forename: San-Mei
authors.initials: SM
authors.lastname: Chen
authors.forename: Guo-Rong
authors.initials: GR
authors.lastname: Chen
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000378:metabolism; D000818:Animals; D001786:Blood Glucose; D003474:Curcumin / Q000494:pharmacology*; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology*; D006321:Heart / Q000187:drug effects*; D007333:Insulin Resistance; D008297:Male; D009206:Myocardium / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013311:Streptozocin
chemical_list: D001786:Blood Glucose; D013311:Streptozocin; D055372:AMP-Activated Protein Kinases; D003474:Curcumin