title: Collagen gel contraction as a measure of fibroblast function in carpal tunnel syndrome.
abstract: Noninflammatory subsynovial connective tissue (SSCT) fibrosis with nerve compression is a prominent feature of carpal tunnel syndrome (CTS). Studies have shown that SSCT matrix synthesis and material property changes in CTS are associated with increased activity of transforming growth factor (TGF)-β1. The aim of this study were to (1) investigate the ability of SSCT fibroblasts from CTS patients and unaffected individuals to contract a collagen gel ring and (2) determine how the addition of TGF-β1 affects this ability. SSCT fibroblasts from three normal cadavers and three age-matched female patients who had undergone surgery for CTS were used. Results showed patient cell-seeded gels had a significantly higher contraction rate (p < 0.001) than control cells, and fully contracted gel rings possessed a significantly higher tensile strength (p = 0.003) and stiffness (p < 0.001). Furthermore, TGF-β1 significantly intensified contraction rate (p < 0.001), tensile strength (p < 0.001), and stiffness (p < 0.001). In conclusion, SSCT cells from normal donors and CTS patients contract collagen gel rings differently, and this ability is affected by TGF-β1 treatment. This cell-seeded collagen gel model may be useful for developing new methods of stopping or eliminating the effect of TGF-β1 on the SSCT fibroblasts and surrounding matrix, which might aid in the identification of medical treatment for CTS.
authors.affiliation: Biomechanics & Tendon and Soft Tissue Biology Laboratory, Division of Orthopedic Research, Mayo Clinic, 200 First Street SW, Rochester, MN, 55905.
authors.forename: Tai-Hua
authors.initials: TH
authors.lastname: Yang
authors.forename: Andrew R
authors.initials: AR
authors.lastname: Thoreson
authors.forename: Anne
authors.initials: A
authors.lastname: Gingery
authors.forename: Kai-Nan
authors.initials: KN
authors.lastname: An
authors.forename: Dirk R
authors.initials: DR
authors.lastname: Larson
authors.forename: Chunfeng
authors.initials: C
authors.lastname: Zhao
authors.forename: Peter C
authors.initials: PC
authors.lastname: Amadio
mesh_terms: D000368:Aged; D002349:Carpal Tunnel Syndrome / Q000378:metabolism* / Q000473:pathology; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry*; D005260:Female; D005347:Fibroblasts / Q000378:metabolism* / Q000473:pathology; D005782:Gels / Q000737:chemistry; D006801:Humans; D008875:Middle Aged; D013718:Tensile Strength; D053773:Transforming Growth Factor beta1 / Q000096:biosynthesis*
chemical_list: D005782:Gels; C508960:TGFB1 protein, human; D053773:Transforming Growth Factor beta1; D003094:Collagen
keywords: TGF-β1; carpal tunnel syndrome; collagen gel contraction; fibrosis; subsynovial connective tissue
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