title: Role of hla-B alleles and clinical presentation of b27 negative spondyloarthritis patients from mumbai, Western India.
abstract: Seronegative spondyloarthritis (SpA) are variably associated with HLA-B∗27 antigen. HLA-B∗27 negative SpA has also been reported from different parts of the world. There is paucity of data on this entity from Indian subcontinent. We studied 100 consecutively diagnosed HLA-B27 negative spondyloarthritis patients from a tertiary care center in India. Modified New York Criteria for ankylosing spondylitis (AS) and ESSG criteria for SpA were used for diagnosing patients. HLA-B∗27 typing was done by an in-house PCR-SSP technique in SpA patients to exclude B∗27 positive patients and PCR-SSOP technique was used to type 100 B∗27 negative SpA patients and 100 controls from the same ethnicity. Frequency of B∗07 was significantly increased (B∗07: % PF 54 versus 18; OR 5.348; 95% CI 2.808-10.186; P value 1.14E - 07), whereas frequency of B∗40 was significantly decreased (B∗40: % PF 17 versus 32; OR 0.435; 95% CI 0.222-0.850; P value 0.013) when compared with B∗27 negative controls. Among 100 SpA patients, 47 were undifferentiated spondyloarthritis and 33 patients were reactive arthritis patients. 40% of the patients were suffering from polyarticular arthritis, 35% had pauciarticular arthritis with knee joint, hip joint, ankle joint, and SI joint involvement. We conclude that B∗07 was significantly associated with B27 negative spondyloarthropathy from Western India and majority of B∗27 negative patients were uSpA.
authors.affiliation: Microbiology and Immunology Division, National Institute of Nutrition (ICMR), Jamai-Osmania, Tarnaka, Hyderabad, Andhra Pradesh 500007, India.
authors.forename: Devaraj J
authors.initials: DJ
authors.lastname: Parasannanavar
authors.affiliation: Department of Medicine, King Edward Memorial Hospital, Parel, Mumbai 400 012, India.
authors.forename: Anjali
authors.initials: A
authors.lastname: Rajadhyaksha
authors.affiliation: National Institute of Immunohaematology (ICMR), King Edward Memorial Hospital, 13th Floor, N.M.S. Building, Parel, Mumbai 400 012, India.
authors.forename: Kanjaksha
authors.initials: K
authors.lastname: Ghosh
references.citation: Br J Rheumatol. 1983 Nov;22(4 Suppl 2):134-5
references.pmid: 6606464
references.citation: Hum Immunol. 2003 May;64(5):562-6
references.pmid: 12691707
references.citation: Semin Arthritis Rheum. 1994 Dec;24(3):190-210
references.pmid: 7534942
references.citation: J Assoc Physicians India. 1998 Apr;46(4):345-50
references.pmid: 11273314
references.citation: Arthritis Rheum. 1991 Oct;34(10):1218-27
references.pmid: 1930310
references.citation: J Rheumatol. 2003 Dec;30(12):2632-7
references.pmid: 14719206
references.citation: Br J Biomed Sci. 2002;59(1):38-41
references.pmid: 12000185
references.citation: Br J Biomed Sci. 2009;66(1):25-7
references.pmid: 19348123
references.citation: Tissue Antigens. 1995 Mar;45(3):169-76
references.pmid: 7761976
references.citation: J Rheumatol. 1995 Oct;22(10):1884-90
references.pmid: 8991986
references.citation: Tissue Antigens. 2003 Aug;62(2):175-8
references.pmid: 12889998
references.citation: Arthritis Rheum. 1989 Sep;32(9):1135-41
references.pmid: 2789045
references.citation: Rev Med Interne. 1984 Jun;5(2):128-35
references.pmid: 6591321
references.citation: Johns Hopkins Med J. 1977 Oct;141(4):193-7
references.pmid: 909215
references.citation: Lancet. 1973 Apr 28;1(7809):904-7
references.pmid: 4123836
references.citation: Arthritis Rheum. 1984 Apr;27(4):361-8
references.pmid: 6231933
references.citation: Indian J Pathol Microbiol. 1996 Jan;39(1):19-25
references.pmid: 8755127
references.citation: J Rheumatol. 2010 Nov;37(11):2402-8
references.pmid: 20682668
references.citation: J Rheumatol. 1985 Oct;12(5):964-6
references.pmid: 3878883
references.citation: Arthritis. 2013;2013:504109
references.pmid: 24490069
references.citation: Br J Rheumatol. 1983 Nov;22(4 Suppl 2):129-33
references.pmid: 6606463
references.citation: Semin Arthritis Rheum. 1973;3(1):55-78
references.pmid: 4581554
references.citation: Lancet. 1978 Jun 24;1(8078):1370-1
references.pmid: 78144
references.citation: Arthritis Rheum. 1978 Jun;21(5):528-30
references.pmid: 666873
references.citation: Arthritis Rheum. 1995 Nov;38(11):1672-7
references.pmid: 7488289
references.citation: Rheumatology (Oxford). 2004 Jul;43(7):839-42
references.pmid: 15113995