title: Homosubtypic and heterosubtypic antibodies against highly pathogenic avian influenza H5N1 recombinant proteins in H5N1 survivors and non-H5N1 subjects.
abstract: Six recombinant vaccinia viruses containing HA, NA, NP, M or NS gene insert derived from a highly pathogenic avian influenza H5N1 virus, and the recombinant vaccinia virus harboring plasmid backbone as the virus control were constructed. The recombinant proteins were characterized for their expression and subcellular locations in TK(-) cells. Antibodies to the five recombinant proteins were detected in all 13 sequential serum samples collected from four H5N1 survivors during four years of follow-up; and those directed to rVac-H5 HA and rVac-NA proteins were found in higher titers than those directed to the internal proteins as revealed by indirect immunofluorescence assay. Although all 28 non-H5N1 subjects had no neutralizing antibodies against H5N1 virus, they did have cross-reactive antibodies to those five recombinant proteins. A significant increase in cross-reactive antibody titer to rVac-H5 HA and rVac-NA was found in paired blood samples from patients infected with the 2009 pandemic virus.
authors.affiliation: Siriraj Influenza Cooperative Research Center, Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok-noi, Bangkok 10700, Thailand; Center for Emerging and Neglected Infectious Disease, Mahidol University, Nakhon Pathom 73170, Thailand.
authors.forename: Pirom
authors.initials: P
authors.lastname: Noisumdaeng
authors.affiliation: Siriraj Influenza Cooperative Research Center, Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok-noi, Bangkok 10700, Thailand.
authors.forename: Phisanu
authors.initials: P
authors.lastname: Pooruk
authors.affiliation: Siriraj Influenza Cooperative Research Center, Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok-noi, Bangkok 10700, Thailand.
authors.forename: Jarunee
authors.initials: J
authors.lastname: Prasertsopon
authors.affiliation: Department of Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok-noi, Bangkok 10700, Thailand.
authors.forename: Susan
authors.initials: S
authors.lastname: Assanasen
authors.affiliation: Department of Disease Control, Ministry of Public Health, Nonthaburi 11000, Thailand.
authors.forename: Rungrueng
authors.initials: R
authors.lastname: Kitphati
authors.affiliation: Siriraj Influenza Cooperative Research Center, Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok-noi, Bangkok 10700, Thailand; Center for Emerging and Neglected Infectious Disease, Mahidol University, Nakhon Pathom 73170, Thailand.
authors.forename: Prasert
authors.initials: P
authors.lastname: Auewarakul
authors.affiliation: Siriraj Influenza Cooperative Research Center, Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok-noi, Bangkok 10700, Thailand; Center for Emerging and Neglected Infectious Disease, Mahidol University, Nakhon Pathom 73170, Thailand. Electronic address: pilaipan.put@mahidol.ac.th.
authors.forename: Pilaipan
authors.initials: P
authors.lastname: Puthavathana
mesh_terms: D000328:Adult; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood*; D002648:Child; D002675:Child, Preschool; D003429:Cross Reactions; D005822:Genetic Vectors; D006801:Humans; D053124:Influenza A Virus, H5N1 Subtype / Q000235:genetics / Q000276:immunology*; D007251:Influenza, Human / Q000276:immunology* / Q000821:virology; D008875:Middle Aged; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D017741:Survivors; D014616:Vaccinia virus / Q000235:genetics; D015678:Viral Structural Proteins / Q000235:genetics / Q000276:immunology*
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D011994:Recombinant Proteins; D015678:Viral Structural Proteins
keywords: Heterosubtypic antibodies; Highly pathogenic avian influenza (HPAI) H5N1 virus; Homosubtypic antibodies; Immunofluorescence assay; Influenza recombinant proteins; Recombinant vaccinia virus; Western blot assay