title: Influence of sulfate and phosphate on the deposition of plasmid DNA on silica and alumina-coated surfaces.
abstract: The influence of sulfate and phosphate on the deposition kinetics of plasmid DNA on solid surfaces was examined at a constant 300mM ionic strength in both NaCl-Na2SO4 and NaCl-NaH2PO4-Na2HPO4 mixing solutions with varied sulfate and phosphate concentrations at pH 6.0 by utilizing a quartz crystal microbalance with dissipation (QCM-D). Two representative solid surfaces, both silica and alumina-coated surfaces, were concerned in this study. To better understand the effects of sulfate and phosphate on plasmid DNA deposition, QCM-D data were complemented by diffusion coefficients and zeta potentials of DNA as a function of examined solution conditions. The presence of sulfate and phosphate in solutions decreased the deposition efficiencies of plasmid DNA on both silica and alumina-coated surfaces. Moreover, the deposition efficiencies decreased with increasing concentrations of sulfate/phosphate. With sulfate/phosphate ions present in solutions, the deposition kinetics of plasmid DNA on both silica and alumina-coated surfaces were mainly controlled by classic Derjaguin-Landau-Verwey-Overbeek (DLVO) interactions.
authors.affiliation: The Key Laboratory of Water and Sediment Sciences, Ministry of Education; College of Environmental Sciences and Engineering, Peking University, Beijing 100871, PR China.
authors.forename: Xiaopeng
authors.initials: X
authors.lastname: Min
authors.affiliation: The Key Laboratory of Water and Sediment Sciences, Ministry of Education; College of Environmental Sciences and Engineering, Peking University, Beijing 100871, PR China.
authors.forename: Peng
authors.initials: P
authors.lastname: Han
authors.affiliation: The Key Laboratory of Water and Sediment Sciences, Ministry of Education; College of Environmental Sciences and Engineering, Peking University, Beijing 100871, PR China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Yang
authors.affiliation: Department of Mineral Resources and Energy Engineering, Chonbuk National University, Jeonju, Jeonbuk 561-756, Republic of Korea.
authors.forename: Hyunjung
authors.initials: H
authors.lastname: Kim
authors.affiliation: The Key Laboratory of Water and Sediment Sciences, Ministry of Education; College of Environmental Sciences and Engineering, Peking University, Beijing 100871, PR China. Electronic address: tongmeiping@pku.edu.cn.
authors.forename: Meiping
authors.initials: M
authors.lastname: Tong
mesh_terms: D000537:Aluminum Oxide / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D004247:DNA / Q000378:metabolism*; D004058:Diffusion; D009994:Osmolar Concentration; D010710:Phosphates / Q000737:chemistry*; D010957:Plasmids / Q000378:metabolism*; D011107:Polylysine / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry*; D012996:Solutions; D055672:Static Electricity; D013431:Sulfates / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D020099:Coated Materials, Biocompatible; D010710:Phosphates; D012996:Solutions; D013431:Sulfates; D011107:Polylysine; D012822:Silicon Dioxide; D004247:DNA; D000537:Aluminum Oxide
keywords: Alumina; Phosphate; Plasmid DNA; QCM-D; Silica; Sulfate