title: Rapid detection of sildenafil analogue in Eurycoma longifolia products using a new two-tier procedure of the near infrared (NIR) spectra database.
abstract: A simple and cost-effective two-tier drug screening procedure comprises a 'dedicated' NIR spectral database of common medicines and a 'unified' database was developed to detect the sildenafil analogue in Eurycoma longifolia products. Diffuse reflectance spectra of ten commercial herbal products containing E. longifolia were obtained over the wavelength range of 1100-2500 nm. The spectral search of two products purchased via the internet against a dedicated database of reputable E. longifolia products have resulted in the similarity index of more than 0.1 which indicated significantly different spectra. Further searches against the unified database showed a close match to the spectra of drug containing sildenafil citrate suggesting the presence of a sildenafil analogue. This finding was supported by clustering of these spectra in the PCA score plot within 5% significance level. This approach has alleviated the use of reference product or standard active for direct comparison and has a potential to be used for adulterated food and drugs detection.
authors.affiliation: UCL School of Pharmacy, 29-39 Brunswick Square, London WC1N 1AX, UK. Electronic address: mazlina.said@pharmacy.ukm.my.
authors.forename: Mazlina Mohd
authors.initials: MM
authors.lastname: Said
authors.affiliation: UCL School of Pharmacy, 29-39 Brunswick Square, London WC1N 1AX, UK.
authors.forename: Simon
authors.initials: S
authors.lastname: Gibbons
authors.affiliation: UCL School of Pharmacy, 29-39 Brunswick Square, London WC1N 1AX, UK.
authors.forename: Anthony C
authors.initials: AC
authors.lastname: Moffat
authors.affiliation: UCL School of Pharmacy, 29-39 Brunswick Square, London WC1N 1AX, UK.
authors.forename: Mire
authors.initials: M
authors.lastname: Zloh
mesh_terms: D016208:Databases, Factual; D035701:Eurycoma / Q000737:chemistry*; D010879:Piperazines / Q000737:chemistry*; D025341:Principal Component Analysis / Q000379:methods*; D011687:Purines / Q000737:chemistry; D000068677:Sildenafil Citrate; D019265:Spectroscopy, Near-Infrared / Q000191:economics* / Q000379:methods*; D013449:Sulfonamides / Q000737:chemistry*
chemical_list: D010879:Piperazines; D011687:Purines; D013449:Sulfonamides; D000068677:Sildenafil Citrate
keywords: Eurycoma longifolia; NIRs; Principal component analysis; SIMCA; Sildenafil analogue; Spectral database