title: Propolis modulates fibronectin expression in the matrix of thermal injury.
abstract: The aim of the study was to assess the propolis effect on fibronectin metabolism in the course of burn wounds healing process. A model of burn wound healing of pig skin was applied. The amount of the released glycoprotein was assessed by a surface plasmon resonance. The profile of extracted fibronectin components was also assessed by an electrophoresis in polyacrylamide gel, with a subsequent immunodetection by Western Blotting. Propolis burn treatment decreased the release of fibronectin components from healing wounds in relation to damages treated with silver sulfadiazine. The main reason of decreased extraction of fibronectin components from wounds treated with propolis was a substantial decrease of degradation product release of the mentioned glycoprotein, which was observed particularly from the 3rd to 5th day of the repair. Wounds treatment with propolis demonstrated, especially in relation to damages treated with silver sulfadiazine, the decreased release of synthesized fibronectin molecules. The obtained results suggest that propolis modifies fibronectin metabolism in the course of wound healing process. The influence of propolis is reflected in prevention of fibronectin biosynthesis as well as its degradation in the wound area. The above-mentioned metabolic changes may decrease the risk of complications in the repair wounds process.
authors.affiliation: Department of Community Pharmacy, Medical University of Silesia in Katowice, 41-200 Sosnowiec, Poland.
authors.forename: Pawel
authors.identifier: 0000-0001-7387-9587
authors.initials: P
authors.lastname: Olczyk
authors.affiliation: Department of Clinical Chemistry and Laboratory Diagnostics, Medical University of Silesia in Katowice, 41-200 Sosnowiec, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Komosinska-Vassev
authors.affiliation: Department of Clinical Chemistry and Laboratory Diagnostics, Medical University of Silesia in Katowice, 41-200 Sosnowiec, Poland.
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Wisowski
authors.affiliation: Department of Clinical Chemistry and Laboratory Diagnostics, Medical University of Silesia in Katowice, 41-200 Sosnowiec, Poland.
authors.forename: Lukasz
authors.initials: L
authors.lastname: Mencner
authors.affiliation: Center of Experimental Medicine, Medical University of Silesia in Katowice, 40-752 Katowice, Poland.
authors.forename: Jerzy
authors.initials: J
authors.lastname: Stojko
authors.affiliation: Department of Clinical Chemistry and Laboratory Diagnostics, Medical University of Silesia in Katowice, 41-200 Sosnowiec, Poland.
authors.forename: Ewa M
authors.initials: EM
authors.lastname: Kozma
mesh_terms: D000818:Animals; D002056:Burns / Q000188:drug therapy*; D005353:Fibronectins / Q000096:biosynthesis*; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D011429:Propolis / Q000008:administration & dosage*; D012867:Skin / Q000187:drug effects* / Q000293:injuries; D013552:Swine; D014945:Wound Healing / Q000187:drug effects
chemical_list: D005353:Fibronectins; D011429:Propolis
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