title: CacyBP/SIP protein is important for the proliferation of human glioma cells.
abstract: Recently, calcyclin-binding protein or Siah-1-interacting protein (CacyBP/SIP), a component of a novel ubiquitinylation pathway, could regulate the β-catenin degradation (Fukushima et al., Immunity 2006, 24, 29-39). However, the potential role of CacyBP/SIP itself in human glioma cells has not been clarified. Here, we found that CacyBP/SIP was expressed highly in human glioma tissues. Silencing of CacyBP/SIP by short-hairpin RNA severely suppressed the proliferation of human glioma cell U251, which was at least partly mediated by downregulation of phospho-Akt (p-Akt) and phospho-β-catenin (p-β-catenin) as well as upregulation of p53 and p21. Furthermore, overexpression of CacyBP/SIP obviously promoted the proliferation of human glioma U251, which exhibited the exactly contrary trend in the expression of p-Akt, p-β-catenin, p53, and p21. Taken together, these findings suggest that CacyBP/SIP plays important roles in the proliferation of human glioma cell which might be involved in the development of human glioma.
authors.affiliation: Department of Neurosurgery, Affiliated Hospital of Xuzhou Medical College, Jiangsu, People's Republic of China.
authors.forename: Hengliang
authors.initials: H
authors.lastname: Shi
authors.forename: Yong
authors.initials: Y
authors.lastname: Gao
authors.forename: Yuan
authors.initials: Y
authors.lastname: Tang
authors.forename: Yuxuan
authors.initials: Y
authors.lastname: Wu
authors.forename: Hui
authors.initials: H
authors.lastname: Gong
authors.forename: Jin
authors.initials: J
authors.lastname: Du
authors.forename: Bao
authors.initials: B
authors.lastname: Zheng
authors.forename: Jinxia
authors.initials: J
authors.lastname: Hu
authors.forename: Qiong
authors.initials: Q
authors.lastname: Shi
authors.forename: Rutong
authors.initials: R
authors.lastname: Yu
mesh_terms: D015153:Blotting, Western; D001921:Brain / Q000378:metabolism*; D001932:Brain Neoplasms / Q000378:metabolism / Q000473:pathology*; D002135:Calcium-Binding Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D002453:Cell Cycle; D049109:Cell Proliferation; D005434:Flow Cytometry; D005910:Glioma / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D034741:RNA, Small Interfering / Q000235:genetics; D014407:Tumor Cells, Cultured
chemical_list: C491778:CACYBP protein, human; D002135:Calcium-Binding Proteins; D034741:RNA, Small Interfering
keywords: CacyBP/SIP; cell cycle; glioma; proliferation