title: Effect of photoperiod, light intensity and carbon sources on biomass and lipid productivities of Isochrysis galbana.
abstract: Biomass and lipid productivities of Isochrysis galbana were optimized using nutrients of molasses (4, 8, 12 g l(-1)), glucose (4, 8, 12 g l(-1)), glycerol (4, 8, 12 g l(-1)) and yeast extract (2 g l(-1)). Combinations of carbon sources at different ratios were evaluated in which the alga was grown at three different light intensities (50, 100 and 150 μmol m(-2) s(-1)) under the influence of three different photoperiod cycles (12/12, 18/6 and 24/0 h light/dark). A maximum cell density of 8.35 g l(-1) with 32 % (w/w) lipid was achieved for mixotrophic growth at 100 μmol m(-2) s(-1) and 18/6 h light/dark with molasses/glucose (20:80 w/w). Mixotrophic cultivation using molasses, glucose and glycerol was thus effective for the cultivation of I. galbana.
authors.affiliation: Centre for Biotechnology, Anna University, Chennai, 600 025, India.
authors.forename: Srinivasan
authors.initials: S
authors.lastname: Babuskin
authors.forename: Kesavan
authors.initials: K
authors.lastname: Radhakrishnan
authors.forename: Packirisamy Azhagu Saravana
authors.initials: PA
authors.lastname: Babu
authors.forename: Meenakshisundaram
authors.initials: M
authors.lastname: Sivarajan
authors.forename: Muthusamy
authors.initials: M
authors.lastname: Sukumar
mesh_terms: D052818:Autotrophic Processes / Q000187:drug effects; D018533:Biomass; D002244:Carbon / Q000494:pharmacology*; D002452:Cell Count; D005227:Fatty Acids / Q000096:biosynthesis; D005947:Glucose / Q000494:pharmacology; D005990:Glycerol / Q000494:pharmacology; D058087:Haptophyta / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism* / Q000528:radiation effects*; D008027:Light; D008055:Lipids / Q000096:biosynthesis*; D017440:Photoperiod
chemical_list: D005227:Fatty Acids; D008055:Lipids; D002244:Carbon; D005947:Glucose; D005990:Glycerol