title: Yulangsan polysaccharide attenuates withdrawal symptoms and regulates the NO pathway in morphine-dependent rats.
abstract: Yulangsan polysaccharide (YLSP) has been utilized as a phytomedicine to managing nervous dysfunction in China. Thus, this study aimed to evaluate the potential YLSP-mediated detoxification role against morphine dependence in rats. The results indicated that the morphine dependence model significantly increased withdrawal symptoms, levels of NO and NOS (P<0.05). Furthermore, monoaminergic neurotransmitters, including DA and NE, were detected at elevated levels in the ventral tegmental area (VTA), hippocampus (HIP) and prefrontal cortex (PFC), respectively, while the level of DA was decreased and NE was increased in the nucleus accumbens (NAc). Conversely, YLSP administration significantly reversed naloxone-induced withdrawal symptoms, expression of brain NO and NOS, and monoaminergic neurotransmitters (P<0.05). Interestingly, YLSP shows an even more effective trend in attenuating withdrawal symptoms than does clonidine, although without a significant difference. These findings indicate that YLSP attenuation of the naloxone-induced withdrawal symptoms of morphine dependence may be mediated by regulation of the NO pathway and modulation of monoaminergic neurotransmitters.
authors.affiliation: Department of Pharmacology, Guangxi Medical University, Nanning, Guangxi 530021, PR China.
authors.forename: Chunxia
authors.initials: C
authors.lastname: Chen
authors.affiliation: Department of Pharmacology, Guangxi Medical University, Nanning, Guangxi 530021, PR China.
authors.forename: Zhihuan
authors.initials: Z
authors.lastname: Nong
authors.affiliation: Department of Pharmacology, Guangxi Medical University, Nanning, Guangxi 530021, PR China.
authors.forename: Jiangchun
authors.initials: J
authors.lastname: Huang
authors.affiliation: Department of Pharmacology, Guangxi Medical University, Nanning, Guangxi 530021, PR China.
authors.forename: Zhaoni
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Pharmacology, Guangxi Medical University, Nanning, Guangxi 530021, PR China.
authors.forename: Shijun
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Pharmacology, Guangxi Medical University, Nanning, Guangxi 530021, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Jiao
authors.affiliation: Department of Pharmacology, Guangxi Medical University, Nanning, Guangxi 530021, PR China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Pharmacology, Guangxi Medical University, Nanning, Guangxi 530021, PR China. Electronic address: huangrenbin518@163.com.
authors.forename: Renbin
authors.initials: R
authors.lastname: Huang
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D004298:Dopamine / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use; D008297:Male; D009020:Morphine / Q000009:adverse effects*; D009021:Morphine Dependence / Q000188:drug therapy* / Q000378:metabolism / Q000503:physiopathology; D009270:Naloxone; D009569:Nitric Oxide / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000378:metabolism; D009638:Norepinephrine / Q000378:metabolism; D011134:Polysaccharides / Q000627:therapeutic use*; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction; D013375:Substance Withdrawal Syndrome / Q000188:drug therapy* / Q000378:metabolism / Q000503:physiopathology
chemical_list: D004365:Drugs, Chinese Herbal; D011134:Polysaccharides; D009569:Nitric Oxide; D009270:Naloxone; D009020:Morphine; D019001:Nitric Oxide Synthase; D004298:Dopamine; D009638:Norepinephrine
keywords: Monoaminergic neurotransmitters; Morphine; NO pathway; Withdrawal symptoms; Yulangsan polysaccharide