title: Relationship between mitochondrial DNA copy number and SIRT1 expression in porcine oocytes.
abstract: The present study assessed the effect of resveratrol on the expression of SIRT1 and mitochondrial quality and quantity in porcine oocytes. Supplementing the maturation medium with 20 µM resveratrol increased the expression of SIRT1, and enhanced mitochondrial functions, as observed from the increased ATP content and mitochondrial membrane potential. Addition of resveratrol also improved the ability of oocytes to develop into the blastocyst stage following activation. The effects of resveratrol on mitochondrial number were examined by comparing the mitochondrial DNA copy number (Mt number) between group of oocytes collected from the same donor gilt ovaries. Supplementing the maturation medium with only resveratrol did not affect the Mt number in the oocytes. However, supplementing the maturation medium with 10 µM MG132, a proteasome inhibitor, significantly increased the amount of ubiquitinated proteins and Mt number by 12 and 14%, respectively. In addition, when resveratrol was added to the medium containing MG132, the Mt number increased significantly by 39%, this effect was diminished by the addition of the SIRT1 inhibitor EX527. Furthermore, supplementing the medium with MG132 and EX527 did not affect Mt number. The mean SIRT1 expression in 20 oocytes was significantly and positively correlated with the Mt number in oocytes collected from the same donor. This study suggests that the expression of SIRT1 is associated with the Mt number in oocytes. In addition, activation of SIRT1 by resveratrol enhances the biosynthesis and degradation of mitochondria in oocytes, thereby replenishing and improving mitochondrial function and the developmental ability of oocytes.
authors.affiliation: Tokyo University of Agriculture, Funako, Atugi City, Japan.
authors.forename: Daichi
authors.initials: D
authors.lastname: Sato
authors.affiliation: Tokyo University of Agriculture, Funako, Atugi City, Japan.
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Itami
authors.affiliation: Tokyo University of Agriculture, Funako, Atugi City, Japan.
authors.forename: Hidetaka
authors.initials: H
authors.lastname: Tasaki
authors.affiliation: Tokyo University of Agriculture, Funako, Atugi City, Japan.
authors.forename: Shun
authors.initials: S
authors.lastname: Takeo
authors.affiliation: Tokyo University of Agriculture, Funako, Atugi City, Japan.
authors.forename: Takehito
authors.initials: T
authors.lastname: Kuwayama
authors.affiliation: Tokyo University of Agriculture, Funako, Atugi City, Japan.
authors.forename: Hisataka
authors.initials: H
authors.lastname: Iwata
mesh_terms: D000818:Animals; D004272:DNA, Mitochondrial / Q000235:genetics*; D005260:Female; D018628:Gene Dosage / Q000187:drug effects; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D009865:Oocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D000077185:Resveratrol; D056564:Sirtuin 1 / Q000378:metabolism*; D013267:Stilbenes / Q000494:pharmacology; D013552:Swine
chemical_list: D004272:DNA, Mitochondrial; D013267:Stilbenes; D056564:Sirtuin 1; D000077185:Resveratrol
references.citation: Fertil Steril. 2006 Mar;85(3):584-91
references.pmid: 16500323
references.citation: Mol Reprod Dev. 2013 Jul;80(7):508-21
references.pmid: 23712640
references.citation: Reprod Domest Anim. 2014 Feb;49(1):101-8
references.pmid: 24102925
references.citation: Biochem Biophys Res Commun. 2013 Aug 23;438(2):270-6
references.pmid: 23891692
references.citation: Biol Reprod. 2007 Feb;76(2):327-35
references.pmid: 17035641
references.citation: Fertil Steril. 2014 Feb;101(2):577-86
references.pmid: 24314921
references.citation: Cell Death Differ. 2013 Jan;20(1):31-42
references.pmid: 22743996
references.citation: Mol Hum Reprod. 2013 Jul;19(7):444-50
references.pmid: 23468533
references.citation: J Cell Mol Med. 2009 Oct;13(10):4176-84
references.pmid: 19267881
references.citation: Reprod Fertil Dev. 2011;23(3):424-32
references.pmid: 21426860
references.citation: Cardiovasc Res. 2010 Apr 1;86(1):103-12
references.pmid: 19959541
references.citation: J Biol Chem. 2010 Sep 3;285(36):27879-90
references.pmid: 20573959
references.citation: Cell. 2012 Feb 3;148(3):421-33
references.pmid: 22304913
references.citation: J Cell Biol. 2010 Dec 27;191(7):1367-80
references.pmid: 21173115
references.citation: Dev Biol. 1987 Oct;123(2):364-74
references.pmid: 2443405
references.citation: Mol Reprod Dev. 2007 Oct;74(10):1278-86
references.pmid: 17290429
references.citation: J Cell Sci. 2010 Aug 1;123(Pt 15):2533-42
references.pmid: 20940129
references.citation: J Pharmacol Exp Ther. 2010 May;333(2):593-601
references.pmid: 20103585
references.citation: Cell Death Differ. 2013 Jan;20(1):21-30
references.pmid: 22722335
references.citation: Hum Mol Genet. 2012 Mar 1;21(5):978-90
references.pmid: 22080835
references.citation: Science. 2012 Aug 31;337(6098):1062-5
references.pmid: 22936770
references.citation: Mitochondrion. 2013 Nov;13(6):755-61
references.pmid: 23583953
references.citation: J Assist Reprod Genet. 2012 Jul;29(7):637-42
references.pmid: 22527902
references.citation: Proc Natl Acad Sci U S A. 2008 Mar 4;105(9):3374-9
references.pmid: 18296641
references.citation: Mol Hum Reprod. 2001 May;7(5):425-9
references.pmid: 11331664
references.citation: Commun Integr Biol. 2011 Jul;4(4):382-4
references.pmid: 21966552
references.citation: Am J Physiol Heart Circ Physiol. 2009 Jul;297(1):H13-20
references.pmid: 19429820
references.citation: Nature. 2004 Jun 17;429(6993):771-6
references.pmid: 15175761
references.citation: Exp Gerontol. 2010 Aug;45(7-8):503-11
references.pmid: 20451598
references.citation: J Reprod Fertil Suppl. 1993;48:61-73
references.pmid: 8145215
references.citation: Blood Press. 2010 Jun;19(3):196-205
references.pmid: 20429690
references.citation: Cell Metab. 2012 Jun 6;15(6):838-47
references.pmid: 22682224
references.citation: Zygote. 2014 Nov;22(4):431-9
references.pmid: 23232110
references.citation: Hum Reprod. 2005 Mar;20(3):593-7
references.pmid: 15608038
references.citation: PLoS One. 2013 May 31;8(5):e64955
references.pmid: 23741435
references.citation: Hum Mol Genet. 2011 May 1;20(9):1726-37
references.pmid: 21296869
references.citation: Cell. 2004 Feb 20;116(4):551-63
references.pmid: 14980222
references.citation: Cell Metab. 2012 May 2;15(5):675-90
references.pmid: 22560220
references.citation: J Reprod Dev. 2014 Apr 24;60(2):92-9
references.pmid: 24390595
references.citation: Mol Cells. 2009 Nov 30;28(5):407-15
references.pmid: 19936627