title: Effects of consumption of rooibos (Aspalathus linearis) and a rooibos-derived commercial supplement on hepatic tissue injury by tert-butyl hydroperoxide in Wistar rats.
abstract: This study investigated the antioxidative effect of rooibos herbal tea and a rooibos-derived commercial supplement on tert-butyl hydroperoxide- (t-BHP-) induced oxidative stress in the liver. Forty male Wistar rats consumed fermented rooibos, unfermented rooibos, a rooibos-derived commercial supplement, or water for 10 weeks, while oxidative stress was induced during the last 2 weeks via intraperitoneal injection of 30 µmole of t-BHP per 100 g body weight. None of the beverages impaired the body weight gain of the respective animals. Rats consuming the rooibos-derived commercial supplement had the highest (P < 0.05) daily total polyphenol intake (169 mg/day) followed by rats consuming the unfermented rooibos (93.4 mg/day) and fermented rooibos (73.1 mg/day). Intake of both the derived supplement and unfermented rooibos restored the t-BHP-induced reduction and increased (P < 0.05) the antioxidant capacity status of the liver, while not impacting on lipid peroxidation. The rooibos herbal tea did not affect the hepatic antioxidant enzymes, except fermented rooibos that caused a decrease (P < 0.05) in superoxide dismutase activity. This study confirms rooibos herbal tea as good dietary antioxidant sources and, in conjunction with its many other components, offers a significantly enhanced antioxidant status of the liver in an induced oxidative stress situation.
authors.affiliation: Oxidative Stress Research Centre, Department of Biomedical Sciences, Faculty of Health and Wellness Sciences, Cape Peninsula University of Technology, P.O. Box 1906, Bellville 7535, South Africa.
authors.forename: B D
authors.initials: BD
authors.lastname: Canda
authors.affiliation: Oxidative Stress Research Centre, Department of Biomedical Sciences, Faculty of Health and Wellness Sciences, Cape Peninsula University of Technology, P.O. Box 1906, Bellville 7535, South Africa.
authors.forename: O O
authors.initials: OO
authors.lastname: Oguntibeju
authors.affiliation: Oxidative Stress Research Centre, Department of Biomedical Sciences, Faculty of Health and Wellness Sciences, Cape Peninsula University of Technology, P.O. Box 1906, Bellville 7535, South Africa.
authors.forename: J L
authors.initials: JL
authors.lastname: Marnewick
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D036903:Aspalathus / Q000737:chemistry*; D001628:Beverages; D001835:Body Weight; D019587:Dietary Supplements; D015227:Lipid Peroxidation; D008099:Liver / Q000201:enzymology / Q000473:pathology*; D008297:Male; D018384:Oxidative Stress; D059808:Polyphenols / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D020122:tert-Butylhydroperoxide
chemical_list: D000975:Antioxidants; D059808:Polyphenols; D020122:tert-Butylhydroperoxide
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