title: Akt inhibitor MK2206 prevents influenza pH1N1 virus infection in vitro.
abstract: The influenza pH1N1 virus caused a global flu pandemic in 2009 and continues manifestation as a seasonal virus. Better understanding of the virus-host cell interaction could result in development of better prevention and treatment options. Here we show that the Akt inhibitor MK2206 blocks influenza pH1N1 virus infection in vitro. In particular, at noncytotoxic concentrations, MK2206 alters Akt signaling and inhibits endocytic uptake of the virus. Interestingly, MK2206 is unable to inhibit H3N2, H7N9, and H5N1 viruses, indicating that pH1N1 evolved specific requirements for efficient infection. Thus, Akt signaling could be exploited further for development of better therapeutics against pH1N1 virus.
authors.affiliation: Institute for Molecular Medicine Finland, University of Helsinki, Helsinki, Finland.
authors.forename: Oxana V
authors.initials: OV
authors.lastname: Denisova
authors.affiliation: Institute of Biotechnology, University of Helsinki, Helsinki, Finland Finnish Institute of Occupational Health, University of Helsinki, Helsinki, Finland.
authors.forename: Sandra
authors.initials: S
authors.lastname: Söderholm
authors.affiliation: Institute of Biomedicine, University of Helsinki, Helsinki, Finland.
authors.forename: Salla
authors.initials: S
authors.lastname: Virtanen
authors.affiliation: Institute for Molecular Medicine Finland, University of Helsinki, Helsinki, Finland.
authors.forename: Carina
authors.initials: C
authors.lastname: Von Schantz
authors.affiliation: Institute for Molecular Medicine Finland, University of Helsinki, Helsinki, Finland.
authors.forename: Dmitrii
authors.initials: D
authors.lastname: Bychkov
authors.affiliation: Institute of Biomedicine, University of Helsinki, Helsinki, Finland.
authors.forename: Elena
authors.initials: E
authors.lastname: Vashchinkina
authors.affiliation: Institute for Molecular Medicine Finland, University of Helsinki, Helsinki, Finland.
authors.forename: Jens
authors.initials: J
authors.lastname: Desloovere
authors.affiliation: Department of Infectious Disease Surveillance and Control, National Institute for Health and Welfare, University of Helsinki, Helsinki, Finland.
authors.forename: Janne
authors.initials: J
authors.lastname: Tynell
authors.affiliation: Department of Infectious Disease Surveillance and Control, National Institute for Health and Welfare, University of Helsinki, Helsinki, Finland.
authors.forename: Niina
authors.initials: N
authors.lastname: Ikonen
authors.affiliation: Institute of Immunology, Centre de Recherche Public de la Santé/Laboratoire National de Santé, Luxembourg, Luxembourg.
authors.forename: Linda L
authors.initials: LL
authors.lastname: Theisen
authors.affiliation: Institute of Biotechnology, University of Helsinki, Helsinki, Finland.
authors.forename: Tuula A
authors.initials: TA
authors.lastname: Nyman
authors.affiliation: Finnish Institute of Occupational Health, University of Helsinki, Helsinki, Finland.
authors.forename: Sampsa
authors.initials: S
authors.lastname: Matikainen
authors.affiliation: Institute for Molecular Medicine Finland, University of Helsinki, Helsinki, Finland.
authors.forename: Olli
authors.initials: O
authors.lastname: Kallioniemi
authors.affiliation: Department of Infectious Disease Surveillance and Control, National Institute for Health and Welfare, University of Helsinki, Helsinki, Finland.
authors.forename: Ilkka
authors.initials: I
authors.lastname: Julkunen
authors.affiliation: Institute of Immunology, Centre de Recherche Public de la Santé/Laboratoire National de Santé, Luxembourg, Luxembourg.
authors.forename: Claude P
authors.initials: CP
authors.lastname: Muller
authors.affiliation: VIB Inflammation Research Center and Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium.
authors.forename: Xavier
authors.initials: X
authors.lastname: Saelens
authors.affiliation: Department of Anatomy and Structural Biology and Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, Bronx, New York, USA.
authors.forename: Vladislav V
authors.initials: VV
authors.lastname: Verkhusha
authors.affiliation: Institute for Molecular Medicine Finland, University of Helsinki, Helsinki, Finland Department of Environmental Research, Siauliai University, Siauliai, Lithuania denis.kainov@helsinki.fi.
authors.forename: Denis E
authors.initials: DE
authors.lastname: Kainov
mesh_terms: D002460:Cell Line; D016207:Cytokines / Q000378:metabolism; D006575:Heterocyclic Compounds, 3-Ring / Q000494:pharmacology*; D054884:Host-Pathogen Interactions / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D053118:Influenza A Virus, H1N1 Subtype; D007251:Influenza, Human / Q000517:prevention & control* / Q000821:virology; D008969:Molecular Sequence Data; D051058:Oncogene Protein v-akt / Q000037:antagonists & inhibitors*; D010750:Phosphoproteins / Q000378:metabolism; D011480:Protease Inhibitors / Q000494:pharmacology*; D034741:RNA, Small Interfering / Q000235:genetics; D014162:Transfection; D010948:Viral Plaque Assay
chemical_list: D016207:Cytokines; D006575:Heterocyclic Compounds, 3-Ring; C548887:MK 2206; D010750:Phosphoproteins; D011480:Protease Inhibitors; D034741:RNA, Small Interfering; D051058:Oncogene Protein v-akt
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