title: [The influence of fibronectin on the formation of multi-cellular spheroid of ovarian cancer].
abstract: OBJECTIVE
  To investigate the role of Fibronectin in the formation of multi-cellular spheroid of ovarian cancer and the integrin receptor involved in the process.
  
  
  METHODS
  In vitro model of multi-cellular spheroid of SKOV3 was constructed by liguid overlay technique. The influence of fibronectin on the formation of the spheroid was observed. The gene expressions of potential integrin receptors were examined from the levels of mRNA and protein using real time reverse transcription PCR and Western-blot.
  
  
  RESULTS
  Fibronctin stimulated the formation of multi-cellular spheroid of ovarian cancer larger than 250 microm. fibronectin suppressed the expression of subtype of integrin receptor ITGA5.
  
  
  CONCLUSIONS
  Fibronectin can enhance the formation of multi-cellular spheroid of ovarian cancer. The subtype of integrin receptor ITGA5 is probably involved in the process.
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Xie
authors.forename: Shan-Ling
authors.initials: SL
authors.lastname: Liu
authors.forename: He
authors.initials: H
authors.lastname: Wang
authors.forename: Lin
authors.initials: L
authors.lastname: Lin
authors.forename: Hong-Mei
authors.initials: HM
authors.lastname: Zhu
authors.forename: Jian-Jun
authors.initials: JJ
authors.lastname: Zhang
authors.forename: Ying
authors.initials: Y
authors.lastname: Zheng
mesh_terms: D045744:Cell Line, Tumor; D005260:Female; D005353:Fibronectins / Q000494:pharmacology*; D015870:Gene Expression; D006801:Humans; D039482:Integrin alpha5 / Q000378:metabolism; D010051:Ovarian Neoplasms / Q000473:pathology*; D012333:RNA, Messenger; D018874:Spheroids, Cellular / Q000473:pathology*
chemical_list: D005353:Fibronectins; D039482:Integrin alpha5; D012333:RNA, Messenger