title: Silver nanoparticle-embedded poly(vinyl pyrrolidone) hydrogel dressing: gamma-ray synthesis and biological evaluation.
abstract: Silver nanoparticle (nAg)-embedded poly(vinyl pyrrolidone) (PVP) hydrogels, to be used as antibacterial wound dressings, were prepared by γ-irradiation at various doses: 25, 35, and 45 kGy. The formation and characteristics of the silver nanoparticles were investigated with a UV-vis spectrophotometer, transmission electron microscopy, and scanning electron microscopy with energy-dispersive X-ray. The hydrogels were characterized for physical and biological properties. Based on the antibacterial determination, the 1 and 5 mM nAg-embedded PVP hydrogels were effective, with 99.99% bactericidal activity at 12 and 6 h, respectively. The indirect cytotoxicity evaluation based on 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay indicated that both the neat and the nAg-embedded PVP hydrogels were non-toxic to mouse fibroblasts (L929). The 5 mM nAg-embedded PVP hydrogels not only provided a clean, moist environment for wound healing, but also effectively prevented bacterial infection and enhanced wound recovery.
authors.affiliation: a Center of Excellence on Petrochemical and Materials Technology (PETROMAT), The Petroleum Petrochemical College, Chulalongkorn University , Bangkok 10330 , Thailand.
authors.forename: Thitikan
authors.initials: T
authors.lastname: Khampieng
authors.forename: Pasakorn
authors.initials: P
authors.lastname: Brikshavana
authors.forename: Pitt
authors.initials: P
authors.lastname: Supaphol
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D001458:Bandages / Q000382:microbiology; D002460:Cell Line; D005720:Gamma Rays; D020100:Hydrogels / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D051379:Mice; D010316:Particle Size; D011205:Povidone / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*; D014867:Water / Q000737:chemistry; D014945:Wound Healing / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D020100:Hydrogels; D014867:Water; D012834:Silver; D011205:Povidone
keywords: hydrogels; poly(vinyl pyrrolidone); silver nanoparticles; wound dressing; γ-irradiation