title: The endoplasmic reticulum and unfolded protein response in the control of mammalian recombinant protein production.
abstract: The endoplasmic reticulum (ER) of eukaryotic cells is involved in the synthesis and processing of proteins and lipids in the secretory pathway. These processing events that proteins undergo in the ER may present major limiting steps for recombinant protein production. Increased protein synthesis, accumulation of improperly processed or mis-folded protein can induce ER stress. To cope with ER stress, the ER has quality control mechanisms, such as the unfolded protein response (UPR) and ER-associated degradation to restore homeostasis. ER stress and UPR activation trigger multiple physiological cellular changes. Here we review cellular mechanisms that cope with ER stress and illustrate how this knowledge can be applied to increase the efficiency of recombinant protein expression.
authors.affiliation: Faculty of Life Sciences, The Michael Smith Building, University of Manchester, Oxford Road, Manchester, M13 9PT, UK.
authors.forename: Hirra
authors.initials: H
authors.lastname: Hussain
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Maldonado-Agurto
authors.forename: Alan J
authors.initials: AJ
authors.lastname: Dickson
mesh_terms: D000818:Animals; D001343:Autophagy; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D059865:Endoplasmic Reticulum Stress; D006801:Humans; D008322:Mammals / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis*; D056811:Unfolded Protein Response
chemical_list: D011994:Recombinant Proteins