title: High-throughput particle separation and concentration using spiral inertial filtration.
abstract: A spiral inertial filtration (SIFT) device that is capable of high-throughput (1 ml/min), high-purity particle separation while concentrating recovered target particles by more than an order of magnitude is reported. This device is able to remove large fractions of sample fluid from a microchannel without disruption of concentrated particle streams by taking advantage of particle focusing in inertial spiral microfluidics, which is achieved by balancing inertial lift forces and Dean drag forces. To enable the calculation of channel geometries in the SIFT microsystem for specific concentration factors, an equivalent circuit model was developed and experimentally validated. Large particle concentration factors were then achieved by maintaining either the average fluid velocity or the Dean number throughout the entire length of the channel during the incremental removal of sample fluid. The SIFT device was able to separate MCF7 cells spiked into whole blood from the non-target white blood cells (WBC) with a recovery of nearly 100% while removing 93% of the sample volume, which resulted in a concentration enhancement of the MCF7 cancer cells by a factor of 14.
authors.affiliation: Fischell Department of Bioengineering, University of Maryland, College Park, Maryland 20742, USA.
authors.forename: Jeffrey M
authors.initials: JM
authors.lastname: Burke
authors.affiliation: Fischell Department of Bioengineering, University of Maryland, College Park, Maryland 20742, USA.
authors.forename: Rebecca E
authors.initials: RE
authors.lastname: Zubajlo
authors.affiliation: Department of Mechanical Engineering, University of Maryland, College Park, Maryland 20742, USA.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Smela
authors.affiliation: Fischell Department of Bioengineering, University of Maryland, College Park, Maryland 20742, USA.
authors.forename: Ian M
authors.initials: IM
authors.lastname: White
references.citation: Lab Chip. 2009 Nov 7;9(21):3038-46
references.pmid: 19823716
references.citation: J Chromatogr A. 2011 Jul 8;1218(27):4138-43
references.pmid: 21176909
references.citation: Lab Chip. 2010 Aug 21;10(16):2057-61
references.pmid: 20563345
references.citation: Lab Chip. 2008 Nov;8(11):1906-14
references.pmid: 18941692
references.citation: Anal Chem. 2010 May 1;82(9):3862-7
references.pmid: 20373755
references.citation: Comput Methods Biomech Biomed Engin. 2011 Jun;14(6):549-60
references.pmid: 21331958
references.citation: Science. 2004 May 14;304(5673):987-90
references.pmid: 15143275
references.citation: Biomicrofluidics. 2013 Aug 08;7(4):44116
references.pmid: 24404049
references.citation: Lab Chip. 2011 Sep 7;11(17):2827-34
references.pmid: 21804970
references.citation: Biomed Microdevices. 2011 Feb;13(1):97-105
references.pmid: 20865451
references.citation: Proc Natl Acad Sci U S A. 2006 May 9;103(19):7228-33
references.pmid: 16645036
references.citation: Lab Chip. 2012 Oct 21;12(20):3952-60
references.pmid: 22868446
references.citation: Biomed Microdevices. 2012 Feb;14(1):25-33
references.pmid: 21898009
references.citation: Lab Chip. 2012 Nov 7;12(21):4336-43
references.pmid: 22899210
references.citation: Lab Chip. 2011 Mar 21;11(6):1118-25
references.pmid: 21298159
references.citation: Lab Chip. 2009 Oct 21;9(20):2973-80
references.pmid: 19789752
references.citation: Lab Chip. 2009 Apr 7;9(7):935-8
references.pmid: 19294304
references.citation: Proc Natl Acad Sci U S A. 2008 Nov 25;105(47):18165-70
references.pmid: 19015523
references.citation: Lab Chip. 2010 Feb 7;10(3):274-80
references.pmid: 20090998
references.citation: ASAIO J. 2005 Sep-Oct;51(5):585-90
references.pmid: 16322722
references.citation: Lab Chip. 2006 Jul;6(7):871-80
references.pmid: 16804591
references.citation: Lab Chip. 2011 Jun 7;11(11):1870-8
references.pmid: 21505682
references.citation: Anal Chem. 2012 Nov 6;84(21):9324-31
references.pmid: 23025404
references.citation: Lab Chip. 2006 Apr;6(4):500-7
references.pmid: 16572212
references.citation: New J Phys. 2009 Jul 1;11:75025
references.pmid: 20862272
references.citation: Anal Chem. 2008 Mar 15;80(6):2204-11
references.pmid: 18275222
references.citation: Proc Natl Acad Sci U S A. 2007 Nov 27;104(48):18892-7
references.pmid: 18025477
references.citation: Lab Chip. 2008 Jul;8(7):1079-86
references.pmid: 18584082
references.citation: Lab Chip. 2011 Apr 7;11(7):1359-67
references.pmid: 21336340
references.citation: Biomed Microdevices. 2010 Apr;12(2):187-95
references.pmid: 19946752
references.citation: Biomicrofluidics. 2011 Jun;5(2):22206
references.pmid: 21918676
references.citation: Lab Chip. 2009 Jan 7;9(1):87-90
references.pmid: 19209339
references.citation: Lab Chip. 2009 Apr 7;9(7):939-48
references.pmid: 19294305
references.citation: Lab Chip. 2007 Feb;7(2):170-8
references.pmid: 17268618
references.citation: Phys Fluids (1994). 2012 Mar;24(3):32001
references.pmid: 22454556
references.citation: Biomicrofluidics. 2013 Sep 05;7(5):54101
references.pmid: 24404064
references.citation: Lab Chip. 2012 Feb 7;12(3):515-45
references.pmid: 22179505
references.citation: Biomicrofluidics. 2013 Jan 07;7(1):11802
references.pmid: 24396523
references.citation: Sci Rep. 2013;3:1259
references.pmid: 23405273
references.citation: Biotechnol Bioeng. 2010 Oct 1;107(2):302-11
references.pmid: 20589838
references.citation: Anal Chem. 2009 Oct 15;81(20):8459-65
references.pmid: 19761190
references.citation: Conf Proc IEEE Eng Med Biol Soc. 2004;2004:2627-30
references.pmid: 17270814
references.citation: Anal Chem. 2006 Feb 15;78(4):1357-62
references.pmid: 16478134
references.citation: Lab Chip. 2012 Apr 21;12(8):1427-30
references.pmid: 22327803
references.citation: Lab Chip. 2005 Jul;5(7):778-84
references.pmid: 15970972
references.citation: Anal Chem. 2005 Feb 1;77(3):933-7
references.pmid: 15679363
references.citation: Lab Chip. 2009 May 7;9(9):1193-9
references.pmid: 19370236
references.citation: Proc Natl Acad Sci U S A. 2010 Oct 26;107(43):18392-7
references.pmid: 20930119
references.citation: Lab Chip. 2014 Jan 7;14(1):128-37
references.pmid: 23949794
references.citation: J Chromatogr A. 2007 Aug 31;1162(2):126-31
references.pmid: 17618636
references.citation: Anal Chem. 2007 Jul 15;79(14):5117-23
references.pmid: 17569501
references.citation: Biomed Microdevices. 2010 Jun;12(3):485-97
references.pmid: 20204703