title: [Simultaneous isolation and primary culture of rat hepatocytes, hepatic stellate cells, Kupffer's cells and hepatic sinus endothelial cells].
abstract: OBJECTIVE
  To establish a method for simultaneous isolation and primary culture of rat hepatocytes, hepatic stellate cells, Kupffer's cells and hepatic sinus endothelial cells.
  
  
  METHODS
  By combining in situ perfusion, in vitro perfusion, density gradient centrifugation and differential adhesion, primary rat hepatocytes, hepatic stellate cells, Kupffer's cells and hepatic sinus endothelial cells were obtained. The purity of these cells were assessed with morphological observation, immunofluorescent staining and ink phagocytosis assay.
  
  
  RESULTS
  We successfully obtained the 4 primary cells simultaneously by combining in situ perfusion with in vitro perfusion, density gradient centrifugation, and differential attachment. The cell yield rate, cell viability and purity all met requirements for the subsequent cell experiment.
  
  
  CONCLUSIONS
  The combined cell isolation and culture method is feasible to isolate primary rat hepatocytes, hepatic stellate cells, Kupffer's cells and hepatic sinus endothelial cells simultaneously.
authors.affiliation: Department of Emergency Medicine, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.E-mail: sl-devil@163.com.
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.forename: Shuangming
authors.initials: S
authors.lastname: Cai
authors.forename: Lili
authors.initials: L
authors.lastname: Zhang
authors.forename: Xu
authors.initials: X
authors.lastname: Li
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002469:Cell Separation; D042783:Endothelial Cells / Q000166:cytology*; D055166:Hepatic Stellate Cells / Q000166:cytology*; D022781:Hepatocytes / Q000166:cytology*; D007728:Kupffer Cells / Q000166:cytology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley