title: MicroRNA-23a has minimal effect on endurance exercise-induced adaptation of mouse skeletal muscle.
abstract: Skeletal muscles contain several subtypes of myofibers that differ in contractile and metabolic properties. Transcriptional control of fiber-type specification and adaptation has been intensively investigated over the past several decades. Recently, microRNA (miRNA)-mediated posttranscriptional gene regulation has attracted increasing attention. MiR-23a targets key molecules regulating contractile and metabolic properties of skeletal muscle, such as myosin heavy-chains and peroxisome proliferator-activated receptor gamma, coactivator 1 alpha (PGC-1α). In the present study, we analyzed the skeletal muscle phenotype of miR-23a transgenic (miR-23a Tg) mice to explore whether forced expression of miR-23a affects markers of mitochondrial content, muscle fiber composition, and muscle adaptations induced by 4 weeks of voluntary wheel running. When compared with wild-type mice, protein markers of mitochondrial content, including PGC-1α, and cytochrome c oxidase complex IV (COX IV), were significantly decreased in the slow soleus muscle, but not the fast plantaris muscle of miR-23a Tg mice. There was a decrease in type IId/x fibers only in the soleus muscle of the Tg mice. Following 4 weeks of voluntary wheel running, there was no difference in the endurance exercise capacity as well as in several muscle adaptive responses including an increase in muscle mass, capillary density, or the protein content of myosin heavy-chain IIa, PGC-1α, COX IV, and cytochrome c. These results show that miR-23a targets PGC-1α and regulates basal metabolic properties of slow but not fast twitch muscles. Elevated levels of miR-23a did not impact on whole body endurance capacity or exercise-induced muscle adaptations in the fast plantaris muscle.
authors.affiliation: Division of Regenerative Medical Engineering, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo, 113-0033, Japan.
authors.forename: Shogo
authors.initials: S
authors.lastname: Wada
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Kato
authors.forename: Shuji
authors.initials: S
authors.lastname: Sawada
authors.forename: Katsuji
authors.initials: K
authors.lastname: Aizawa
authors.forename: Jong-Hoon
authors.initials: JH
authors.lastname: Park
authors.forename: Aaron P
authors.initials: AP
authors.lastname: Russell
authors.forename: Takashi
authors.initials: T
authors.lastname: Ushida
authors.forename: Takayuki
authors.initials: T
authors.lastname: Akimoto
mesh_terms: D000222:Adaptation, Physiological; D000818:Animals; D003576:Electron Transport Complex IV / Q000235:genetics / Q000378:metabolism; D005260:Female; D051379:Mice; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D018656:Muscle Fibers, Fast-Twitch / Q000378:metabolism* / Q000502:physiology; D018657:Muscle Fibers, Slow-Twitch / Q000378:metabolism* / Q000502:physiology; D018995:Myosin Heavy Chains / Q000235:genetics / Q000378:metabolism; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D005082:Physical Exertion; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D035683:MicroRNAs; C534062:Mirn23b microRNA, mouse; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; C485290:Ppargc1a protein, mouse; D014157:Transcription Factors; D003576:Electron Transport Complex IV; D018995:Myosin Heavy Chains
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