title: Effect of laser phototherapy (λ660 nm) on type I and III collagen expression during wound healing in hypothyroid rats: an immunohistochemical study in a rodent model.
abstract: OBJECTIVE
  The aim of this study was to assess, immunohistochemically, the impact of hypothyroidism and the effect of laser phototherapy on the expression of type I and III collagen during wound healing.
  
  
  BACKGROUND
  Hypothyroidism has been associated with the disruption of the body's metabolism, including the healing process. Laser phototherapy has been shown to be effective in improving wound healing, but its usefulness on enhancing wound healing under hypothyroid condition remains unknown.
  
  
  METHODS
  Using general anesthesia, a standard surgical wound (1 cm(2)) was created on the dorsa of 48 Wistar rats divided into four groups of 12 animals each: control euthyroid (EC), euthyroid plus laser (EL), control hypothyroid (HC), and hypothyroid plus laser (HL). The irradiation with laser GaAlAs [λ660 nm, 40 mW, 1 W/cm(2), continuous wave (CW), ø=0.04 cm(2)] started immediately after surgery and was repeated every other day until end-point of study was reached, and animals were euthanized (i.e., 7 and 14 days). Laser light was applied on four different points (6 J, 150 sec and 150 J/cm(2) per point). Hypothyroidism was induced in rats with propylthiouracil (0.05 g/100 mL) administered orally for 4 weeks and maintained until the end of the experiment. Immunohistochemistry for collagen I and III was performed with EnVision(™) in the specimens removed.
  
  
  RESULTS
  Seven days after the surgery EC, EL, and HL groups showed higher immunoexpression of collagen I and lower immunoexpression of collagen III in the newly formed tissue. There was increased immunoexpression of collagen I in EC when compared with HC (p=0.019). The immunoexpression of collagen III was significantly lower in EL than in EC (p=0.047) and HL (p=0.019). No significant difference was found in the experimental period of 14 days among the groups.
  
  
  CONCLUSIONS
  Laser light therapy performed with the parameters of this investigation increased immunoexpression of collagen type I during tissue repair, and improved the quality of newly formed tissue in the presence of hypothyroidism.
authors.affiliation: 1 Department of Propedeutics and Dental Clinic, School of Dentistry, Federal University of Bahia , Salvador, Bahia, Brazil .
authors.forename: Gardênia Matos
authors.initials: GM
authors.lastname: Paraguassú
authors.forename: Flávia Calo Aquino
authors.initials: FC
authors.lastname: Xavier
authors.forename: Maria Cristina Teixeira
authors.initials: MC
authors.lastname: Cangussu
authors.forename: Maria José Pedreira
authors.initials: MJ
authors.lastname: Ramalho
authors.forename: Patrícia Ramos
authors.initials: PR
authors.lastname: Cury
authors.forename: Jean Nunes
authors.initials: JN
authors.lastname: dos Santos
authors.forename: Antônio Luis Barbosa
authors.initials: AL
authors.lastname: Pinheiro
authors.forename: Luciana Maria Pedreira
authors.initials: LM
authors.lastname: Ramalho
mesh_terms: D000818:Animals; D024042:Collagen Type I / Q000032:analysis*; D024061:Collagen Type III / Q000032:analysis*; D006801:Humans; D007037:Hypothyroidism / Q000503:physiopathology*; D007150:Immunohistochemistry; D028022:Low-Level Light Therapy; D051381:Rats; D017208:Rats, Wistar; D014945:Wound Healing / Q000528:radiation effects*
chemical_list: D024042:Collagen Type I; D024061:Collagen Type III