title: Hec1 inhibition alters spindle morphology and chromosome alignment in porcine oocytes.
abstract: Aneuploidy is caused by incorrect chromosome segregation and can result in cancer or birth defects. The spindle assembly checkpoint (SAC) guarantees proper cell cycle progression. Highly Expressed in Cancer protein 1 (Hec1, also called Ndc80) is the core component of the Ndc80 complex and is involved in regulating both kinetochore-microtubule interactions and the SAC during mitosis in multiple cell types. However, its involvement in pig oocyte meiotic maturation remains uncertain. Thus, we investigated Hec1 expression, localization, and possible functions during porcine oocyte meiosis. Immunofluorescent staining showed that Hec1 was expressed in porcine oocytes and was associated with centromeres at both the metaphase I and metaphase II stages. Disrupting Hec1 function with its inhibitor INH1 resulted in polar body extrusion defects in porcine oocytes. Moreover, inhibiting Hec1 activity also resulted in severe chromosome misalignments and aberrant spindle morphology. Our results showed a unique localization pattern for Hec1 in porcine oocytes and suggested that Hec1 was required for chromosome alignment and spindle organization. Thus, Hec1 might regulate spindle checkpoint activity during mammalian oocyte meiosis.
authors.affiliation: Department of Clinical Laboratory Services, Liuzhou Worker's Hospital, Liuzhou, 545005, China.
authors.forename: Xiaomou
authors.initials: X
authors.lastname: Wei
authors.forename: Chunhai
authors.initials: C
authors.lastname: Gao
authors.forename: Jia
authors.initials: J
authors.lastname: Luo
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Shuhao
authors.initials: S
authors.lastname: Qi
authors.forename: Weijun
authors.initials: W
authors.lastname: Liang
authors.forename: Shengming
authors.initials: S
authors.lastname: Dai
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000494:pharmacology; D002503:Centromere; D020090:Chromosome Segregation / Q000235:genetics; D002875:Chromosomes / Q000235:genetics*; D003599:Cytoskeleton / Q000235:genetics*; D018386:Kinetochores / Q000378:metabolism; D059566:M Phase Cell Cycle Checkpoints; D008540:Meiosis / Q000235:genetics; D008677:Metaphase / Q000235:genetics; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D009865:Oocytes / Q000166:cytology*; D013552:Swine
chemical_list: D001798:Blood Proteins; D008869:Microtubule-Associated Proteins; C053635:factor XIIa inhibitor
references.citation: Science. 2002 Sep 27;297(5590):2267-70
references.pmid: 12351790
references.citation: Curr Biol. 2003 Dec 2;13(23):2103-9
references.pmid: 14654001
references.citation: FEBS Lett. 2007 Jun 19;581(15):2862-9
references.pmid: 17521635
references.citation: Curr Opin Cell Biol. 2008 Feb;20(1):53-63
references.pmid: 18182282
references.citation: Curr Opin Cell Biol. 2005 Feb;17(1):35-46
references.pmid: 15661517
references.citation: Nat Struct Mol Biol. 2007 Jan;14(1):54-9
references.pmid: 17195848
references.citation: J Cell Sci. 2003 Aug 15;116(Pt 16):3347-62
references.pmid: 12829748
references.citation: J Cell Biol. 2010 May 17;189(4):713-23
references.pmid: 20479468
references.citation: Mol Biol Cell. 2005 Feb;16(2):519-31
references.pmid: 15548592
references.citation: J Cell Biol. 2004 Feb 16;164(4):535-46
references.pmid: 14769859
references.citation: Microsc Microanal. 2011 Jun;17(3):431-9
references.pmid: 21600073
references.citation: EMBO J. 2009 Apr 22;28(8):1099-110
references.pmid: 19300438
references.citation: Mol Cell Biol. 1999 Aug;19(8):5417-28
references.pmid: 10409732
references.citation: Curr Biol. 2004 Jan 20;14(2):131-7
references.pmid: 14738735
references.citation: Mol Biol Cell. 2005 May;16(5):2325-38
references.pmid: 15728720
references.citation: Curr Biol. 2008 Nov 25;18(22):1778-84
references.pmid: 19026543
references.citation: Genes Dev. 2004 Sep 15;18(18):2255-68
references.pmid: 15371340
references.citation: Proc Natl Acad Sci U S A. 2008 Oct 28;105(43):16719-24
references.pmid: 18940925
references.citation: EMBO J. 2001 Feb 15;20(4):777-91
references.pmid: 11179222
references.citation: Cell. 2006 Dec 1;127(5):983-97
references.pmid: 17129783
references.citation: Chromosoma. 2001 Sep;110(5):322-34
references.pmid: 11685532
references.citation: Curr Opin Cell Biol. 2010 Feb;22(1):57-67
references.pmid: 20061128
references.citation: Genes Dev. 2003 Jan 1;17(1):101-14
references.pmid: 12514103
references.citation: J Biol Chem. 2004 Mar 26;279(13):13076-85
references.pmid: 14699129
references.citation: J Cell Biol. 2001 Jan 22;152(2):349-60
references.pmid: 11266451
references.citation: Curr Biol. 2008 Nov 25;18(22):1785-91
references.pmid: 19026542
references.citation: Mol Microbiol. 2002 Mar;43(6):1431-43
references.pmid: 11952896