title: Structural elucidation and bioactivity of a novel exopolysaccharide from endophytic Fusarium solani SD5.
abstract: A bioactive exopolysaccharide [EPS (PS-I)], having Mw∼1.87×10(5) Da was produced by submerged culture of an endophytic fungus Fusarium solani SD5. Structural elucidation of the EPS (PS-I) was carried out by a series of experiments. Result indicates the presence of terminal α-L-rhamnopyranosyl, (1→2)-α-L-rhamnopyranosyl, (1→4)-β-D-galactopyranosyl, (1→4,6)-β-D-galactopyranosyl moieties in a molar ratio of nearly 1:1:3:1. TEM image showed fibril structure of the EPS with a diameter of approximately 1 nm. Melting point range of the EPS was found 172-178 °C. The isolated PS-I exhibit in vitro anti inflammatory and anti allergic activity. EPS (1000 μg/ml) protects 55% erythrocytes from hypotonic solution induced membrane lysis. Compound 48/80 induced mast cell degranulation was also protected by 56% with 100 μg/ml EPS.
authors.affiliation: Microbiology Laboratory, Department of Botany and Forestry, Vidyasagar University, Midnapore 721102, West Bengal, India.
authors.forename: Subhadip
authors.initials: S
authors.lastname: Mahapatra
authors.forename: Debdulal
authors.initials: D
authors.lastname: Banerjee
mesh_terms: D000818:Animals; D018926:Anti-Allergic Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D060026:Endophytes; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism; D062610:Fungal Polysaccharides / Q000737:chemistry* / Q000378:metabolism* / Q000494:pharmacology; D005670:Fusarium; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism; D015394:Molecular Structure; D051381:Rats
chemical_list: D018926:Anti-Allergic Agents; D000893:Anti-Inflammatory Agents; D062610:Fungal Polysaccharides