title: [Association of CD(+)4 T lymphocyte count and gingival crevicular fluid prostaglandin E2 with periodontal parameters in HIV-positive periodontitis patients].
abstract: OBJECTIVE
  To investigate the correlation of CD(+)4 T lymphocyte count and prostaglandin E2 (PGE2) in gingival crevicular fluid (GCF) with periodontal status in HIV-positive patients with periodontitis.
  
  
  METHODS
  Twenty subjects were selected according to inclusion criteria. The plasmatic CD(+)4 T lymphocytes were counted. All the individuals were divided into three groups, group A (CD(+)4 T lymphocyte count < 200 cell/mm(3)), group B (200 cell/mm(3) ≤ CD(+)4 T lymphocyte count ≤ 500 cell/mm(3)) and group C (CD(+)4 T lymphocyte count > 500 cell/mm(3)). Periodontal indexes, including plaque index(PLI), bleeding index(BI), attachment level(AL) and probing depth(PD) were recorded.GCF samples were taken from 120 index teeth by means of sterile paper strips.GCF PGE2 levels were determined by radioimmunoassays. Mann-Whitney was used to compare the periodontal indexes and PGE2 levels among the three groups. Partial correlations and Spearman correlations were applied to analyze the correlation of CD(+)4 T lymphocytes count and PGE2 in gingival crevicular fluid with periodontal status.
  
  
  RESULTS
  BI value, PGE2 concentration and total PGE2 were 3.00(2.00), 90.75(30.60) µg/L, 447.58 (243.08) pg in group B, which were higher than those in group A[2.00(1.25), 79.75(30.50) µg/L and 339.52 (200.97) pg respectively] and group C[2.00(1.00), 73.38 (14.83) µg/L and 299.18 (108.33) pg respectively] (P < 0.0167). But the differences of PD and AL among the three groups were not significantly different(P > 0.0167). The correlations were observed between CD(+)4 T lymphocyte count and BI for the subpopulations with CD(+)4 T lymphocyte count <200 cells/mm(3) (r = 0.657, P < 0.05) and between 200-500 cells/mm(3) (r = -0.369, P < 0.05). PGE2 concentration was negatively correlated with BI, PD and AL (P < 0.05), and total PGE2 was positively correlated with PD and AL(P < 0.05).
  
  
  CONCLUSIONS
  There was an association between the periodontal status and CD(+)4 T lymphocyte count in HIV(+) patients.GCF PGE2 level was related to periodontal parameters including BI, PD and AL.
authors.affiliation: Department of Stomatology,Beijing Ditan Hospital Capital Medical University,Beijing 100015, China. Email: asdrtgf@sina.com.
authors.forename: Hongcheng
authors.initials: H
authors.lastname: Jia
authors.forename: Xuan
authors.initials: X
authors.lastname: Wang
authors.forename: Wenhao
authors.initials: W
authors.lastname: Hua
authors.forename: Xiaoguang
authors.initials: X
authors.lastname: Li
authors.forename: Wen
authors.initials: W
authors.lastname: Hou
authors.forename: Qian
authors.initials: Q
authors.lastname: Fu
mesh_terms: D018791:CD4 Lymphocyte Count; D003774:Dental Plaque Index; D015232:Dinoprostone / Q000378:metabolism*; D005883:Gingival Crevicular Fluid / Q000378:metabolism*; D015658:HIV Infections / Q000150:complications*; D006801:Humans; D010512:Periodontal Index; D010518:Periodontitis / Q000276:immunology / Q000821:virology*
chemical_list: D015232:Dinoprostone