title: Direct lineage conversion of pancreatic exocrine to endocrine Beta cells in vivo with defined factors.
abstract: Pancreatic exocrine cells can be directly converted to insulin(+) beta cells by adenoviral-mediated expression of three transcription factors Pdx1, Mafa, and Ngn3 in the adult mouse pancreas (Zhou et al., Nature 455(7213):627-632, 2008). This direct reprogramming approach offers a strategy to replenish beta-cell mass and may be further developed as a potential future treatment for diabetes. Here, we provide a detailed protocol for inducing exocrine to beta-cell reprogramming in mice. We also describe key analyses we routinely use to assess the phenotype and function of reprogrammed cells.
authors.affiliation: Section on Islet Cell and Regenerative Biology, Joslin Diabetes Center Boston, Boston, MA, USA.
authors.forename: Claudia
authors.initials: C
authors.lastname: Cavelti-Weder
authors.forename: Weida
authors.initials: W
authors.lastname: Li
authors.forename: Gordon C
authors.initials: GC
authors.lastname: Weir
authors.forename: Qiao
authors.initials: Q
authors.lastname: Zhou
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D019070:Cell Lineage; D065150:Cellular Reprogramming; D003001:Cloning, Molecular; D003920:Diabetes Mellitus / Q000473:pathology / Q000628:therapy; D050417:Insulin-Secreting Cells / Q000166:cytology* / Q000378:metabolism; D008297:Male; D051379:Mice; D009841:Oligonucleotides / Q000235:genetics; D046790:Pancreas, Exocrine / Q000166:cytology*
chemical_list: D009841:Oligonucleotides