title: Cadmium toxicity induced alterations in the root proteome of green gram in contrasting response towards iron supplement.
abstract: Cadmium signifies a severe threat to crop productivity and green gram is a notably iron sensitive plant which shows considerable variation towards cadmium stress. A gel-based proteomics analysis was performed with the roots of green gram exposed to iron and cadmium combined treatments. The resulting data show that twenty three proteins were down-regulated in iron-deprived roots either in the absence (-Fe/-Cd) or presence (-Fe/+Cd) of cadmium. These down-regulated proteins were however well expressed in roots under iron sufficient conditions, even in the presence of cadmium (+Fe/+Cd). The functional classification of these proteins determined that 21% of the proteins are associated with nutrient metabolism. The other proteins in higher quantities are involved in either transcription or translation regulation, and the rest are involved in biosynthesis metabolism, antioxidant pathways, molecular chaperones and stress response. On the other hand, several protein spots were also absent in roots in response to iron deprivation either in absence (-Fe/-Cd) or presence (-Fe/+Cd) of cadmium but were well expressed in the presence of iron (+Fe/+Cd). Results suggest that green gram plants exposed to cadmium stress are able to change the nutrient metabolic balance in roots, but in the mean time regulate cadmium toxicity through iron supplements.
authors.affiliation: Department of Horticulture, College of Agricultural Life Sciences, Chonnam National University, 300 Young Bong-Dong Buk-Gu, Gwangju 500-757, Korea. sobiyakhan126@gmail.com.
authors.forename: Sowbiya
authors.initials: S
authors.lastname: Muneer
authors.affiliation: Faculty of Forestry, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia. kur.hakeem@upm.edu.my.
authors.forename: Khalid Rehman
authors.initials: KR
authors.lastname: Hakeem
authors.affiliation: Faculty of Forestry, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia. rozimohd@upm.edu.my.
authors.forename: Rozi
authors.initials: R
authors.lastname: Mohamed
authors.affiliation: Department of Horticulture, College of Agricultural Life Sciences, Chonnam National University, 300 Young Bong-Dong Buk-Gu, Gwangju 500-757, Korea. leetag@chonnam.ac.kr.
authors.forename: Jeong Hyun
authors.initials: JH
authors.lastname: Lee
mesh_terms: D002104:Cadmium / Q000633:toxicity*; D015180:Electrophoresis, Gel, Two-Dimensional; D007887:Fabaceae / Q000187:drug effects* / Q000378:metabolism; D007501:Iron / Q000494:pharmacology*; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism; D020543:Proteome / Q000032:analysis / Q000187:drug effects*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D020543:Proteome; D002104:Cadmium; D007501:Iron
references.citation: J Hazard Mater. 2010 Dec 15;184(1-3):191-203
references.pmid: 20817397
references.citation: Planta. 2006 Oct;224(5):1116-28
references.pmid: 16741749
references.citation: Planta. 2006 May;223(6):1178-90
references.pmid: 16362328
references.citation: Plant Cell. 2002 Jun;14(6):1223-33
references.pmid: 12084823
references.citation: Plant Physiol. 2012 Feb;158(2):790-800
references.pmid: 22184655
references.citation: New Phytol. 2011 Apr;190(1):125-137
references.pmid: 21219335
references.citation: Plant Mol Biol. 1999 May;40(1):37-44
references.pmid: 10394943
references.citation: Plant Cell. 2002 Jun;14(6):1347-57
references.pmid: 12084831
references.citation: J Plant Physiol. 2003 Apr;160(4):387-93
references.pmid: 12756918
references.citation: J Biol Chem. 2002 Apr 5;277(14):12382-7
references.pmid: 11821404
references.citation: Plant Physiol. 1998 Apr;116(4):1413-20
references.pmid: 9536059
references.citation: Trends Plant Sci. 2003 Apr;8(4):188-93
references.pmid: 12711231
references.citation: Plant Cell Rep. 2006 Apr;25(4):365-73
references.pmid: 16344960
references.citation: Mol Cell Proteomics. 2009 Jul;8(7):1579-98
references.pmid: 19321431
references.citation: Amino Acids. 2012 Dec;43(6):2393-416
references.pmid: 22588482
references.citation: J Proteome Res. 2006 Dec;5(12):3301-11
references.pmid: 17137331
references.citation: Proc Natl Acad Sci U S A. 1996 May 28;93(11):5624-8
references.pmid: 8643627
references.citation: Plant Physiol. 2007 Apr;143(4):1761-73
references.pmid: 17337530
references.citation: Plant Physiol. 1998 Mar;116(3):1063-72
references.pmid: 9501139
references.citation: Nature. 1998 Jan 29;391(6666):485-8
references.pmid: 9461215
references.citation: Planta. 2003 Feb;216(4):541-51
references.pmid: 12569395
references.citation: Planta. 2004 Mar;218(5):784-92
references.pmid: 14648120