title: Structure and mechanism of ATP-dependent phospholipid transporters.
abstract: BACKGROUND
  ATP-binding cassette (ABC) transporters and P4-ATPases are two large and seemingly unrelated families of primary active pumps involved in moving phospholipids from one leaflet of a biological membrane to the other.
  
  
  METHODS
  This review aims to identify common mechanistic features in the way phospholipid flipping is carried out by two evolutionarily unrelated families of transporters.
  
  
  CONCLUSIONS
  Both protein families hydrolyze ATP, although they employ different mechanisms to use it, and have a comparable size with twelve transmembrane segments in the functional unit. Further, despite differences in overall architecture, both appear to operate by an alternating access mechanism and during transport they might allow access of phospholipids to the internal part of the transmembrane domain. The latter feature is obvious for ABC transporters, but phospholipids and other hydrophobic molecules have also been found embedded in P-type ATPase crystal structures. Taken together, in two diverse groups of pumps, nature appears to have evolved quite similar ways of flipping phospholipids.
  
  
  CONCLUSIONS
  Our understanding of the structural basis for phospholipid flipping is still limited but it seems plausible that a general mechanism for phospholipid flipping exists in nature. This article is part of a Special Issue entitled Structural biochemistry and biophysics of membrane proteins.
authors.affiliation: Centre for Membrane Pumps in Cells and Disease - PUMPkin, Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark. Electronic address: rlo@plen.ku.dk.
authors.forename: Rosa L
authors.initials: RL
authors.lastname: López-Marqués
authors.affiliation: Centre for Membrane Pumps in Cells and Disease - PUMPkin, Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark.
authors.forename: Lisbeth Rosager
authors.initials: LR
authors.lastname: Poulsen
authors.affiliation: Institute of Plant Biology, University of Zürich, Zollikerstrasse 107, CH-8008 Zürich, Switzerland.
authors.forename: Aurélien
authors.initials: A
authors.lastname: Bailly
authors.affiliation: Department of Biology, Plant Biology, University of Fribourg, Chemin du Musée 10, CH-1700 Fribourg, Switzerland.
authors.forename: Markus
authors.initials: M
authors.lastname: Geisler
authors.affiliation: Centre for Membrane Pumps in Cells and Disease - PUMPkin, Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark.
authors.forename: Thomas Günther
authors.initials: TG
authors.lastname: Pomorski
authors.affiliation: Centre for Membrane Pumps in Cells and Disease - PUMPkin, Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark. Electronic address: palmgren@plen.ku.dk.
authors.forename: Michael G
authors.initials: MG
authors.lastname: Palmgren
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport; D002462:Cell Membrane / Q000378:metabolism*; D006801:Humans; D026901:Membrane Transport Proteins / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D049294:Phospholipid Transfer Proteins / Q000737:chemistry / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism*; D011487:Protein Conformation
chemical_list: D026901:Membrane Transport Proteins; D049294:Phospholipid Transfer Proteins; D010743:Phospholipids; D000255:Adenosine Triphosphate
keywords: ABC transporter; Flippase; Lipid flipping; P-type ATPase; P4-ATPase; Transport