title: Non-equilibrium atmospheric pressure plasmas modulate cell cycle-related gene expressions in melanocytic tumors of RET-transgenic mice.
abstract: The incidence of cutaneous malignant melanoma is increasing at a greater rate than that of any other cancer in the world. However, an effective therapy for malignant melanoma has not been established. Recently, some studies have shown an antitumor effect of non-equilibrium atmospheric pressure plasmas (NEAPPs) in vitro. Here, we examined the in vivo effect of NEAPP on cell cycle regulators, key elements for malignant transformation, in spontaneously developed benign melanocytic tumors in a hairless animal model. NEAPP irradiation decreased expression levels of cell cycle promoters, Cyclin D1, E1 and E2, and increased expression level of a cell cycle repressor, p27(KIP) (1) . Cyclin D1, E1 and E2 and p27(KIP) expression levels were associated with malignant transformation of the benign tumor in the animal model. Our results suggest that NEAPP irradiation suppresses malignant transformation of a benign melanocytic tumor via control of the expression levels of cell cycle regulators.
authors.affiliation: Unit of Environmental Health Sciences, Department of Biomedical Sciences, College of Life and Health Sciences, Chubu University, Kasugai, Aichi, Japan; Department of Occupational and Environmental Health, Nagoya University Graduate School of Medicine, Nagoya, Aichi, Japan.
authors.forename: Ichiro
authors.initials: I
authors.lastname: Yajima
authors.forename: Machiko
authors.initials: M
authors.lastname: Iida
authors.forename: Mayuko Y
authors.initials: MY
authors.lastname: Kumasaka
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Omata
authors.forename: Nobutaka
authors.initials: N
authors.lastname: Ohgami
authors.forename: Jie
authors.initials: J
authors.lastname: Chang
authors.forename: Sahoko
authors.initials: S
authors.lastname: Ichihara
authors.forename: Masaru
authors.initials: M
authors.lastname: Hori
authors.forename: Masashi
authors.initials: M
authors.lastname: Kato
mesh_terms: D000818:Animals; D001128:Argon / Q000627:therapeutic use*; D001274:Atmospheric Pressure; D002453:Cell Cycle / Q000235:genetics / Q000528:radiation effects; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000528:radiation effects; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000528:radiation effects; D004195:Disease Models, Animal; D018450:Disease Progression; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics / Q000528:radiation effects*; D051379:Mice; D008812:Mice, Hairless; D008822:Mice, Transgenic; D009508:Nevus, Pigmented / Q000235:genetics* / Q000473:pathology / Q000532:radiotherapy; D051096:Proto-Oncogene Proteins c-ret / Q000235:genetics*; D011878:Radiotherapy / Q000379:methods*; D012878:Skin Neoplasms / Q000235:genetics* / Q000517:prevention & control / Q000532:radiotherapy
chemical_list: D018797:Cell Cycle Proteins; D001128:Argon; D051096:Proto-Oncogene Proteins c-ret; C494999:Ret protein, mouse
keywords: RET; cell cycle; melanoma; non-equilibrium atmospheric pressure plasma