title: Yeast DEAD box protein Mss116p is a transcription elongation factor that modulates the activity of mitochondrial RNA polymerase.
abstract: DEAD box proteins have been widely implicated in regulation of gene expression. Here, we show that the yeast Saccharomyces cerevisiae DEAD box protein Mss116p, previously known as a mitochondrial splicing factor, also acts as a transcription factor that modulates the activity of the single-subunit mitochondrial RNA polymerase encoded by RPO41. Binding of Mss116p stabilizes paused mitochondrial RNA polymerase elongation complexes in vitro and favors the posttranslocated state of the enzyme, resulting in a lower concentration of nucleotide substrate required to escape the pause; this mechanism of action is similar to that of elongation factors that enhance the processivity of multisubunit RNA polymerases. In a yeast strain in which the RNA splicing-related functions of Mss116p are dispensable, overexpression of RPO41 or MSS116 increases cell survival from colonies that were exposed to low temperature, suggesting a role for Mss116p in enhancing the efficiency of mitochondrial transcription under stress conditions.
authors.affiliation: Department of Cell Biology, Rowan University, School of Osteopathic Medicine, Stratford, New Jersey, USA markovdm@rowan.edu.
authors.forename: Dmitriy A
authors.initials: DA
authors.lastname: Markov
authors.affiliation: Department of Cell Biology, Rowan University, School of Osteopathic Medicine, Stratford, New Jersey, USA.
authors.forename: Ireneusz D
authors.initials: ID
authors.lastname: Wojtas
authors.affiliation: Summer Undergraduate Research Experience Program, Rowan University, School of Osteopathic Medicine, Stratford, New Jersey, USA.
authors.forename: Kassandra
authors.initials: K
authors.lastname: Tessitore
authors.affiliation: Graduate School of Biomedical Sciences, Rowan University, School of Osteopathic Medicine, Stratford, New Jersey, USA.
authors.forename: Simmone
authors.initials: S
authors.lastname: Henderson
authors.affiliation: Department of Cell Biology, Rowan University, School of Osteopathic Medicine, Stratford, New Jersey, USA.
authors.forename: William T
authors.initials: WT
authors.lastname: McAllister
mesh_terms: D001665:Binding Sites / Q000235:genetics; D002470:Cell Survival; D053487:DEAD-box RNA Helicases / Q000235:genetics / Q000302:isolation & purification*; D012321:DNA-Directed RNA Polymerases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology; D015966:Gene Expression Regulation, Fungal; D008928:Mitochondria / Q000201:enzymology* / Q000235:genetics; D024101:Mitochondrial Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D010445:Peptide Elongation Factors / Q000235:genetics; D011485:Protein Binding / Q000235:genetics; D012313:RNA / Q000096:biosynthesis / Q000235:genetics; D012331:RNA, Fungal / Q000096:biosynthesis / Q000235:genetics; D000077278:RNA, Mitochondrial; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D013312:Stress, Physiological / Q000235:genetics; D014157:Transcription Factors; D014158:Transcription, Genetic; D015533:Transcriptional Activation
chemical_list: D024101:Mitochondrial Proteins; D010445:Peptide Elongation Factors; D012331:RNA, Fungal; D000077278:RNA, Mitochondrial; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D012313:RNA; D012321:DNA-Directed RNA Polymerases; C487996:RPO41 protein, S cerevisiae; C058150:MSS116 protein, S cerevisiae; D053487:DEAD-box RNA Helicases
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