title: [Expression of leukocyte-associated Ig-like receptor-1 in children with immune thrombocytopenia].
abstract: OBJECTIVE
  To study the expression of leukocyte-associated Ig-like receptor-1(LAIR-1) in children with immune thrombocytopenia (ITP), in order to explore the possible role of LAIR-1 in the pathogenesis of childhood ITP.
  
  
  METHODS
  Expression levels of LAIR-1 on CD4(+) T cells, CD8(+) T cells and CD19(+)CD20(+) B cells of peripheral blood were measured in 40 children with ITP by flow cytometry. Serum level of solubility LAIR-1 (sLAIR-1) was measured using ELISA. Real-time PCR was used to measure LAIR-1 mRNA expression. Thirty-two healthy children served as the control group.
  
  
  RESULTS
  The percentages of CD19(+)CD20(+) B cells in the ITP group were significantly higher than in the control group (P<0.05). In contrast, the percentage of CD4(+) T cells in the ITP group was significantly lower than in the control group (P<0.05). The expression levels of LAIR-1 on CD4(+) T cells and CD8(+) T cells were significantly lower in the ITP group than in the control group (P<0.05). Serum sLAIR-1 level and LAIR-1 mRNA expression in the ITP group significantly increased compared with the control group (P<0.05).
  
  
  CONCLUSIONS
  LAIR-1 expression on CD4(+) and CD8(+) T cells decreases and serum sLAIR-1 level increases in children with ITP, suggesting that LAIR-1 may play an important role in immune imbalance in these children.
authors.affiliation: Department of Hematology and Oncology, Shenzhen Children's Hospital Affiliated to Zunyi Medical College, Shenzhen, Guangdong 518026, China. licg6336@sina.com.
authors.forename: Mei-Ling
authors.initials: ML
authors.lastname: Huang
authors.forename: Chang-Gang
authors.initials: CG
authors.lastname: Li
authors.forename: Guo-Bing
authors.initials: GB
authors.lastname: Wang
authors.forename: Ying
authors.initials: Y
authors.lastname: Zu
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D016553:Purpura, Thrombocytopenic, Idiopathic / Q000276:immunology*; D012333:RNA, Messenger / Q000032:analysis; D011971:Receptors, Immunologic / Q000097:blood / Q000235:genetics / Q000502:physiology*
chemical_list: D012333:RNA, Messenger; D011971:Receptors, Immunologic; C108073:leukocyte-associated immunoglobulin-like receptor 1