title: KIN enhances stem cell-like properties to promote chemoresistance in colorectal carcinoma.
abstract: Chemotherapy is widely used in colorectal cancer (CRC) treatment, especially in advanced stage patients. However, it is inevitable to develop chemoresistance. Recently, cancer cells acquired stem cell-like properties or cancer stem cells (CSC) were proved to attribute to chemoresistance. Here, we found that KIN protein was elevated in CRC cell lines and tissue specimens as compared to normal controls. Upregulation of KIN positively correlates with the metastatic status of CRC patients. Patients with high KIN expression showed poor prognosis and were with a short survival time. Overexpression of KIN enhanced, while silencing KIN impaired, chemoresistance to oxaliplatin (Ox) or 5-fluorouracil (5-FU) in CRC cell lines. Further investigation demonstrated that overexpression of KIN rendered CRC cells enriching CSC markers and CSC phenotype, and silencing KIN reduced CSC markers and CSC phenotype. Our findings suggest that the KIN level may be a suitable marker for predicting chemotherapy response in CRC, and silencing KIN plus chemotherapy may be a novel therapy for CRC treatment.
authors.affiliation: Department of Surgery, The First Affiliated Hospital, Sun Yat-sen University, 58 Zhongshan Road II, Guangzhou, Guangdong 510080, China.
authors.forename: Miao
authors.initials: M
authors.lastname: Yu
authors.affiliation: Department of Biochemistry and Molecular Biology, Second Military Medical University, 800 Xiangyin Road, Shanghai 200433, China.
authors.forename: Zhenwei
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Surgery, The First Affiliated Hospital, Sun Yat-sen University, 58 Zhongshan Road II, Guangzhou, Guangdong 510080, China.
authors.forename: Honglan
authors.initials: H
authors.lastname: Yu
authors.affiliation: Department of Surgery, The First Affiliated Hospital, Sun Yat-sen University, 58 Zhongshan Road II, Guangzhou, Guangdong 510080, China.
authors.forename: Conglong
authors.initials: C
authors.lastname: Xue
authors.affiliation: Department of Surgery, The First Affiliated Hospital, Sun Yat-sen University, 58 Zhongshan Road II, Guangzhou, Guangdong 510080, China.
authors.forename: Kaitao
authors.initials: K
authors.lastname: Yuan
authors.affiliation: Department of Biochemistry and Molecular Biology, Second Military Medical University, 800 Xiangyin Road, Shanghai 200433, China. Electronic address: miaomy@163.com.
authors.forename: Mingyong
authors.initials: M
authors.lastname: Miao
authors.affiliation: Department of Surgery, The First Affiliated Hospital, Sun Yat-sen University, 58 Zhongshan Road II, Guangzhou, Guangdong 510080, China. Electronic address: shihp@mail.sysu.edu.cn.
authors.forename: Hanping
authors.initials: H
authors.lastname: Shi
mesh_terms: D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000188:drug therapy / Q000473:pathology / Q000503:physiopathology*; D004268:DNA-Binding Proteins / Q000502:physiology*; D019008:Drug Resistance, Neoplasm; D006801:Humans; D014411:Neoplastic Stem Cells / Q000473:pathology / Q000502:physiology*; D016601:RNA-Binding Proteins / Q000502:physiology*; D015854:Up-Regulation
chemical_list: D004268:DNA-Binding Proteins; C489487:KIN protein, human; D016601:RNA-Binding Proteins
keywords: Chemoresistance; Colorectal cancer; KIN; Stem cell-like properties