title: Vaginal fluid IL-6 and IL-8 levels in pregnancies complicated by preterm prelabor membrane ruptures.
abstract: OBJECTIVE
  To determine the vaginal fluid interleukin (IL)-6 and IL-8 concentrations in pregnancies complicated by preterm prelabor rupture of membranes and their correlation to microbial invasion of the amniotic cavity (MIAC) as well as histological chorioamnionitis (HCA).
  
  
  METHODS
  Sixty-eight women with singleton pregnancies were included in this study. Vaginal fluid was collected at the time of admission. IL-6 and IL-8 concentrations in the vaginal fluid were determined using ELISA.
  
  
  RESULTS
  Women with MIAC had higher vaginal fluid IL-6 levels compared to those without MIAC (with MIAC: median 374 pg/mL versus without MIAC: median 174 pg/mL; p = 0.03). IL-8 levels were higher in women with MIAC only in the crude analysis but not after adjustment for gestational age. There was no difference in the IL-6 and IL-8 concentrations between those with and without HCA. Women with both MIAC and HCA had higher IL-6 vaginal fluid levels than those without both MIAC and HCA (with MIAC and HCA: median 466 pg/mL versus without MIAC and HCA: median 178 pg/mL; p = 0.02). IL-8 levels were higher in women with MIAC and HCA only in the crude analysis but not after adjustment for gestational age.
  
  
  CONCLUSIONS
  Vaginal fluid IL-6 but not IL-8 levels reflect the presence of MIAC and both MIAC and HCA.
authors.affiliation: Biomedical Research Center, University Hospital Hradec Kralove , Hradec Kralove , Czech Republic .
authors.forename: Marian
authors.initials: M
authors.lastname: Kacerovsky
authors.forename: Ivana
authors.initials: I
authors.lastname: Musilova
authors.forename: Bo
authors.initials: B
authors.lastname: Jacobsson
authors.forename: Marcela
authors.initials: M
authors.lastname: Drahosova
authors.forename: Helena
authors.initials: H
authors.lastname: Hornychova
authors.forename: Petr
authors.initials: P
authors.lastname: Janku
authors.forename: Martin
authors.initials: M
authors.lastname: Prochazka
authors.forename: Ondrej
authors.initials: O
authors.lastname: Simetka
authors.forename: Ctirad
authors.initials: C
authors.lastname: Andrys
mesh_terms: D000328:Adult; D001826:Body Fluids / Q000737:chemistry* / Q000378:metabolism; D002821:Chorioamnionitis / Q000378:metabolism / Q000473:pathology; D015331:Cohort Studies; D005260:Female; D005322:Premature Rupture of Fetal Membranes / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007231:Infant, Newborn; D015850:Interleukin-6 / Q000032:analysis* / Q000378:metabolism; D016209:Interleukin-8 / Q000032:analysis* / Q000378:metabolism; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000378:metabolism / Q000473:pathology; D014621:Vagina; D055815:Young Adult
chemical_list: C508600:IL6 protein, human; D015850:Interleukin-6; D016209:Interleukin-8
keywords: Histological chorioamnionitis; microbial invasion of the amniotic cavity; preterm delivery