title: PcExl1 a novel acid expansin-like protein from the plant pathogen Pectobacterium carotovorum, binds cell walls differently to BsEXLX1.
abstract: Microbial expansins act on plant cell walls similarly to plant expansins, albeit their loosening activity levels are tenfold lesser compared to plant expansins. We report the characterization of an expansin-like gene from the plant pathogen Pectobacterium carotovorum, named exl1. PcExl1 is an acidic protein that binds cellulose (Avicel), and weakens filter paper. The acidic nature of PcExl1 confers different binding properties when compared to Bacillus subtilis BsEXLX1, which is a basic protein. PcExl1 binding to wheat cell wall increased when acidic components were depleted, reaching a similar level to the binding to Avicel, indicating that cellulose is the target of PcExl1.
authors.affiliation: Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México; Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, México.
authors.forename: Miguel
authors.initials: M
authors.lastname: Olarte-Lozano
authors.affiliation: Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México.
authors.forename: Mario A
authors.initials: MA
authors.lastname: Mendoza-Nuñez
authors.affiliation: Facultad de Ciencias, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, México.
authors.forename: Nina
authors.initials: N
authors.lastname: Pastor
authors.affiliation: Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México.
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Segovia
authors.affiliation: Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, México.
authors.forename: Jorge
authors.initials: J
authors.lastname: Folch-Mallol
authors.affiliation: Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México.
authors.forename: Claudia
authors.initials: C
authors.lastname: Martínez-Anaya
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002473:Cell Wall / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism; D003001:Cloning, Molecular; D015870:Gene Expression; D006026:Glycoside Hydrolases; D006868:Hydrolysis; D008958:Models, Molecular; D016973:Pectobacterium carotovorum / Q000235:genetics / Q000378:metabolism*; D059828:Plant Cells / Q000378:metabolism / Q000382:microbiology; D011485:Protein Binding; D011487:Protein Conformation; D011994:Recombinant Proteins; D013045:Species Specificity; D055672:Static Electricity; D013379:Substrate Specificity
chemical_list: D001426:Bacterial Proteins; D011994:Recombinant Proteins; D002482:Cellulose; D006026:Glycoside Hydrolases
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