title: Trpc2 is expressed in two olfactory subsystems, the main and the vomeronasal system of larval Xenopus laevis.
abstract: Complete segregation of the main olfactory epithelium (MOE) and the vomeronasal epithelium is first observed in amphibians. In contrast, teleost fishes possess a single olfactory surface, in which genetic components of the main and vomeronasal olfactory systems are intermingled. The transient receptor potential channel TRPC2, a marker of vomeronasal neurons, is present in the single fish sensory surface, but is already restricted to the vomeronasal epithelium in a terrestrial amphibian, the red-legged salamander (Plethodon shermani). Here we examined the localization of TRPC2 in an aquatic amphibian and cloned the Xenopus laevis trpc2 gene. We show that it is expressed in both the MOE and the vomeronasal epithelium. This is the first description of a broad trpc2 expression in the MOE of a tetrapod. The expression pattern of trpc2 in the MOE is virtually undistinguishable from that of MOE-specific v2rs, indicating that they are co-expressed in the same neuronal subpopulation.
authors.affiliation: Institute of Neurophysiology and Cellular Biophysics, University of Göttingen, Humboldtallee 23, 37073 Göttingen, Germany.
authors.forename: Alfredo
authors.initials: A
authors.lastname: Sansone
authors.affiliation: Institute of Genetics, University of Cologne, Zülpicher Strasse 47a, 50674 Cologne, Germany.
authors.forename: Adnan S
authors.initials: AS
authors.lastname: Syed
authors.affiliation: Institute of Neurophysiology and Cellular Biophysics, University of Göttingen, Humboldtallee 23, 37073 Göttingen, Germany Center for Nanoscale Microscopy and Molecular Physiology of the Brain (CNMPB), 37073 Göttingen, Germany.
authors.forename: Evangelia
authors.initials: E
authors.lastname: Tantalaki
authors.affiliation: Institute of Genetics, University of Cologne, Zülpicher Strasse 47a, 50674 Cologne, Germany.
authors.forename: Sigrun I
authors.initials: SI
authors.lastname: Korsching
authors.affiliation: Institute of Neurophysiology and Cellular Biophysics, University of Göttingen, Humboldtallee 23, 37073 Göttingen, Germany Center for Nanoscale Microscopy and Molecular Physiology of the Brain (CNMPB), 37073 Göttingen, Germany imanzin@gwdg.de.
authors.forename: Ivan
authors.initials: I
authors.lastname: Manzini
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D007814:Larva / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009831:Olfactory Mucosa / Q000378:metabolism; D055696:Olfactory Perception; D016415:Sequence Alignment; D050052:TRPC Cation Channels / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D014018:Tissue Distribution; D019147:Vomeronasal Organ / Q000378:metabolism; D029867:Xenopus Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D014982:Xenopus laevis / Q000235:genetics / Q000254:growth & development / Q000502:physiology*
chemical_list: D050052:TRPC Cation Channels; C000598337:TRPC2 protein, Xenopus; D029867:Xenopus Proteins
keywords: Amphibians; In situ hybridization; Olfactory organ; RT-PCR
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