title: Rutin and quercetin enhance glucose uptake in L6 myotubes under oxidative stress induced by tertiary butyl hydrogen peroxide.
abstract: Scientific evidence suggests a strong link between the oxidative stress-induced pathways and onset of diabetes and its complications. The present study evaluates the antidiabetic potential of the flavonoids, rutin and its metabolite quercetin under oxidative stress induced by tertiary butyl hydrogen peroxide (TBHP). Our results demonstrate that reactive oxygen species generated by TBHP decreased markedly in the L6 cells on preincubation with flavonoids in a dose-dependent manner and remarkably retrieved the glutathione level which was drastically decreased on oxidative challenge. These flavonoids were also found to prevent lipid peroxidation in L6 myoblast. Flavonoids increased glucose following chronic and acute pretreatment in the presence of oxidative stress. Increased glucose uptake in L6 myotubes was attributed to GLUT 4 translocation, the most downstream factor in the insulin signalling cascade, which increased two to threefold on chronic pretreatment of quercetin (10 μM) and rutin (100 μM).
authors.affiliation: Agroprocessing and Natural Products Division, National Institute for Interdisciplinary Science and Technology (NIIST), CSIR, Industrial Estate, Pappanamcode, Thiruvananthapuram 695019, Kerala, India.
authors.forename: R
authors.initials: R
authors.lastname: Dhanya
authors.affiliation: Agroprocessing and Natural Products Division, National Institute for Interdisciplinary Science and Technology (NIIST), CSIR, Industrial Estate, Pappanamcode, Thiruvananthapuram 695019, Kerala, India.
authors.forename: K B
authors.initials: KB
authors.lastname: Arun
authors.affiliation: Agroprocessing and Natural Products Division, National Institute for Interdisciplinary Science and Technology (NIIST), CSIR, Industrial Estate, Pappanamcode, Thiruvananthapuram 695019, Kerala, India.
authors.forename: H P
authors.initials: HP
authors.lastname: Syama
authors.affiliation: Agroprocessing and Natural Products Division, National Institute for Interdisciplinary Science and Technology (NIIST), CSIR, Industrial Estate, Pappanamcode, Thiruvananthapuram 695019, Kerala, India.
authors.forename: P
authors.initials: P
authors.lastname: Nisha
authors.affiliation: Agroprocessing and Natural Products Division, National Institute for Interdisciplinary Science and Technology (NIIST), CSIR, Industrial Estate, Pappanamcode, Thiruvananthapuram 695019, Kerala, India.
authors.forename: A
authors.initials: A
authors.lastname: Sundaresan
authors.affiliation: Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram 695012, Kerala, India.
authors.forename: T R
authors.initials: TR
authors.lastname: Santhosh Kumar
authors.affiliation: Agroprocessing and Natural Products Division, National Institute for Interdisciplinary Science and Technology (NIIST), CSIR, Industrial Estate, Pappanamcode, Thiruvananthapuram 695019, Kerala, India. Electronic address: jayamurthy@live.com.
authors.forename: P
authors.initials: P
authors.lastname: Jayamurthy
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology; D001692:Biological Transport; D001786:Blood Glucose / Q000378:metabolism*; D002460:Cell Line; D002470:Cell Survival; D005419:Flavonoids / Q000494:pharmacology; D005786:Gene Expression Regulation; D051275:Glucose Transporter Type 4 / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000737:chemistry*; D015227:Lipid Peroxidation; D008856:Microscopy, Fluorescence; D018485:Muscle Fibers, Skeletal / Q000378:metabolism; D018482:Muscle, Skeletal / Q000187:drug effects; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D011794:Quercetin / Q000737:chemistry*; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D012431:Rutin / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D001786:Blood Glucose; D005419:Flavonoids; D051275:Glucose Transporter Type 4; D017382:Reactive Oxygen Species; C495694:Slc2a4 protein, rat; D012431:Rutin; D011794:Quercetin; D006861:Hydrogen Peroxide; D005978:Glutathione
keywords: L6 myotubes; Oxidative stress; Quercetin; Rutin; Tertiary butyl hydrogen peroxide