title: Polyethyleneimine-coating enhances adenoviral transduction of mesenchymal stem cells.
abstract: Mesenchymal stem cells (MSCs) are non-hematopoietic cells with multi-lineage potential, which makes them attractive targets for regenerative medicine applications. Efficient gene transfer into MSCs is essential for basic research in developmental biology and for therapeutic applications involving gene-modification in regenerative medicine. Adenovirus vectors (Advs) can efficiently and transiently introduce an exogenous gene into many cell types via their primary receptors, the coxsackievirus and adenovirus receptors (CARs), but not into MSCs, which lack CAR expression. To overcome this problem, an Adv coated with cationic polymer polyethyleneimine (PEI) was developed. In this study, we demonstrated that PEI coating with an optimal ratio can enhance adenoviral transduction of MSCs without cytotoxicity. We also investigated the physicochemical properties and internalization mechanisms of the PEI-coated Adv. These results could help to evaluate the potentiality of the PEI-coated Adv as a prototype vector for efficient and safe transduction into MSCs.
authors.affiliation: Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Peking Union Medical College Hospital, Center of Excellence in Tissue Engineering Chinese Academy of Medical Sciences, 5# Dongdansantiao, 100005 Beijing, PR China; Institute of Pharmaceutics, Zhejiang University(2), 866 Yuhangtang Road, Hangzhou 310058, PR China.
authors.forename: Xinglei
authors.initials: X
authors.lastname: Yao
authors.affiliation: Department of Oncology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, 1# Shuaifuyuan, 100730 Beijing, PR China.
authors.forename: Na
authors.initials: N
authors.lastname: Zhou
authors.affiliation: Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Peking Union Medical College Hospital, Center of Excellence in Tissue Engineering Chinese Academy of Medical Sciences, 5# Dongdansantiao, 100005 Beijing, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Wan
authors.affiliation: Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Peking Union Medical College Hospital, Center of Excellence in Tissue Engineering Chinese Academy of Medical Sciences, 5# Dongdansantiao, 100005 Beijing, PR China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Su
authors.affiliation: Department of Oncology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, 1# Shuaifuyuan, 100730 Beijing, PR China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Sun
authors.affiliation: Department of Biochemistry and Molecular Biology, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Mizuguchi
authors.affiliation: Department of Toxicology and Safety Science, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Yoshioka
authors.affiliation: Department of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Shinsaku
authors.initials: S
authors.lastname: Nakagawa
authors.affiliation: Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Peking Union Medical College Hospital, Center of Excellence in Tissue Engineering Chinese Academy of Medical Sciences, 5# Dongdansantiao, 100005 Beijing, PR China. Electronic address: chunhuaz@public.tpt.tj.cn.
authors.forename: Robert Chunhua
authors.initials: RC
authors.lastname: Zhao
authors.affiliation: Institute of Pharmaceutics, Zhejiang University(2), 866 Yuhangtang Road, Hangzhou 310058, PR China. Electronic address: gaojianqing@zju.edu.cn.
authors.forename: Jian-Qing
authors.initials: JQ
authors.lastname: Gao
mesh_terms: D000256:Adenoviridae / Q000737:chemistry* / Q000502:physiology; D000818:Animals; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein / Q000235:genetics / Q000502:physiology; D004705:Endocytosis; D005822:Genetic Vectors / Q000737:chemistry*; D008297:Male; D059630:Mesenchymal Stem Cells; D051379:Mice; D008807:Mice, Inbred BALB C; D011094:Polyethyleneimine / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014161:Transduction, Genetic / Q000379:methods*; D014771:Virion / Q000737:chemistry / Q000502:physiology; D053586:Virus Internalization
chemical_list: D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D011094:Polyethyleneimine
keywords: Adenovirus vectors; Gene transduction; Mesenchymal stem cells; Polyethyleneimine