title: Strains and molecular tools for recombinant protein production in Pichia pastoris.
abstract: Within the last two decades, the methylotrophic yeast Pichia pastoris has become an important alternative to E. coli or mammalian cell lines for the production of recombinant proteins. Easy handling, strong promoters, and high cell density cultivations as well as the capability of posttranslational modifications are some of the major benefits of this yeast. The high secretion capacity and low level of endogenously secreted proteins further promoted the rapid development of a versatile Pichia pastoris toolbox. This chapter reviews common and new "Pichia tools" and their specific features. Special focus is given to expression strains, such as different methanol utilization, protease-deficient or glycoengineered strains, combined with application highlights. Different promoters and signal sequences are also discussed.
authors.affiliation: Graz University of Technology, Institute of Molecular Biotechnology, Petersgasse 14, 8010, Graz, Austria.
authors.forename: Michael
authors.initials: M
authors.lastname: Felber
authors.forename: Harald
authors.initials: H
authors.lastname: Pichler
authors.forename: Claudia
authors.initials: C
authors.lastname: Ruth
mesh_terms: D018628:Gene Dosage / Q000235:genetics; D005818:Genetic Engineering / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics; D010843:Pichia / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D013045:Species Specificity
chemical_list: D011994:Recombinant Proteins