title: Fast and solvent-free quantitation of boar taint odorants in pig fat by stable isotope dilution analysis-dynamic headspace-thermal desorption-gas chromatography/time-of-flight mass spectrometry.
abstract: Boar taint is a specific off-odour of boar meat products, known to be caused by at least three unpleasant odorants, with very low odour thresholds. Androstenone is a boar pheromone produced in the testes, whereas skatole and indole originate from the microbial breakdown of tryptophan in the intestinal tract. A new procedure, applying stable isotope dilution analysis (SIDA) and dynamic headspace-thermal desorption-gas chromatography/time-of-flight mass spectrometry (dynHS-TD-GC/TOFMS) for the simultaneous quantitation of these boar taint compounds in pig fat was elaborated and validated in this paper. The new method is characterised by a simple and solvent-free dynamic headspace sampling. The deuterated compounds d3-androstenone, d3-skatole and d6-indole were used as internal standards to eliminate matrix effects. The method validation performed revealed low limits of detection (LOD) and quantitation (LOQ) with high accuracy and precision, thus confirming the feasibility of the new dynHS-TD-GC/TOFMS approach for routine analysis.
authors.affiliation: University of Bonn, Institute for Agricultural Engineering, Work Group on Trace Gas and Odor Measurement, Nussallee 5, D-53115 Bonn, Germany; ELFI Analytik GbR, Massenhausener Strasse 18a, D-85375 Neufahrn, Germany.
authors.forename: Jochen
authors.initials: J
authors.lastname: Fischer
authors.affiliation: University of Bonn, Institute for Agricultural Engineering, Work Group on Trace Gas and Odor Measurement, Nussallee 5, D-53115 Bonn, Germany.
authors.forename: Torsten
authors.initials: T
authors.lastname: Haas
authors.affiliation: University of Bonn, Institute for Agricultural Engineering, Work Group on Trace Gas and Odor Measurement, Nussallee 5, D-53115 Bonn, Germany.
authors.forename: Jan
authors.initials: J
authors.lastname: Leppert
authors.affiliation: University of Bonn, Institute for Agricultural Engineering, Work Group on Trace Gas and Odor Measurement, Nussallee 5, D-53115 Bonn, Germany.
authors.forename: Peter Schulze
authors.initials: PS
authors.lastname: Lammers
authors.affiliation: Five Technologies GmbH, Frauenstrasse 22, 80469 Munich, Germany.
authors.forename: Gerhard
authors.initials: G
authors.lastname: Horner
authors.affiliation: University of Bonn, Department of Nutrition and Food Sciences, Bioanalytics, Endenicher Allee 11-13, D-53115 Bonn, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Wüst
authors.affiliation: University of Bonn, Institute for Agricultural Engineering, Work Group on Trace Gas and Odor Measurement, Nussallee 5, D-53115 Bonn, Germany. Electronic address: boeker@uni-bonn.de.
authors.forename: Peter
authors.initials: P
authors.lastname: Boeker
mesh_terms: D000736:Androstenes / Q000032:analysis; D000818:Animals; D005223:Fats / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D007201:Indicator Dilution Techniques; D007211:Indoles / Q000032:analysis; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D008461:Meat Products / Q000032:analysis; D009812:Odorants / Q000032:analysis*; D012862:Skatole / Q000032:analysis; D034421:Sus scrofa; D013552:Swine
chemical_list: D000736:Androstenes; D005223:Fats; D007211:Indoles; C030374:indole; D012862:Skatole
keywords: Androstenone; Boar taint; Dynamic headspace; Indole; SIDA; Skatole; TOFMS; Thermal desorption