title: Scalable production of highly sensitive nanosensors based on graphene functionalized with a designed G protein-coupled receptor.
abstract: We have developed a novel, all-electronic biosensor for opioids that consists of an engineered μ-opioid receptor protein, with high binding affinity for opioids, chemically bonded to a graphene field-effect transistor to read out ligand binding. A variant of the receptor protein that provided chemical recognition was computationally redesigned to enhance its solubility and stability in an aqueous environment. A shadow mask process was developed to fabricate arrays of hundreds of graphene transistors with average mobility of ∼1500 cm(2) V(-1) s(-1) and yield exceeding 98%. The biosensor exhibits high sensitivity and selectivity for the target naltrexone, an opioid receptor antagonist, with a detection limit of 10 pg/mL.
authors.affiliation: Department of Physics and Astronomy, University of Pennsylvania , Philadelphia, Pennsylvania 19104, United States.
authors.forename: Mitchell B
authors.initials: MB
authors.lastname: Lerner
authors.forename: Felipe
authors.initials: F
authors.lastname: Matsunaga
authors.forename: Gang Hee
authors.initials: GH
authors.lastname: Han
authors.forename: Sung Ju
authors.initials: SJ
authors.lastname: Hong
authors.forename: Jin
authors.initials: J
authors.lastname: Xi
authors.forename: Alexander
authors.initials: A
authors.lastname: Crook
authors.forename: Jose Manuel
authors.initials: JM
authors.lastname: Perez-Aguilar
authors.forename: Yung Woo
authors.initials: YW
authors.lastname: Park
authors.forename: Jeffery G
authors.initials: JG
authors.lastname: Saven
authors.forename: Renyu
authors.initials: R
authors.lastname: Liu
authors.forename: A T Charlie
authors.initials: AT
authors.lastname: Johnson
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D009271:Naltrexone / Q000737:chemistry / Q000302:isolation & purification*; D017450:Receptors, Opioid, mu / Q000037:antagonists & inhibitors* / Q000737:chemistry; D014867:Water / Q000737:chemistry
chemical_list: D017450:Receptors, Opioid, mu; D014867:Water; D009271:Naltrexone; D006108:Graphite
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