title: Genotype-related effect of crowding stress on blood pressure and vascular function in young female rats.
abstract: This study investigated the influence of chronic crowding stress on nitric oxide (NO) production, vascular function and oxidative status in young Wistar-Kyoto (WKY), borderline hypertensive (BHR) and spontaneously hypertensive (SHR) female rats. Five-week old rats were exposed to crowding for two weeks. Crowding elevated plasma corticosterone (P<0.05) and accelerated BP (P<0.01 versus basal) only in BHR. NO production and superoxide concentration were significantly higher in the aortas of control BHR and SHR versus WKY. Total acetylcholine (ACh)-induced relaxation in the femoral artery was reduced in control SHR versus WKY and BHR, and stress did not affect it significantly in any genotype. The attenuation of ACh-induced relaxation in SHR versus WKY was associated with reduction of its NO-independent component. Crowding elevated NO production in all strains investigated but superoxide concentration was increased only in WKY, which resulted in reduced NO-dependent relaxation in WKY. In crowded BHR and SHR, superoxide concentration was either unchanged or reduced, respectively, but NO-dependent relaxation was unchanged in both BHR and SHR versus their respective control group. This study points to genotype-related differences in stress vulnerability in young female rats. The most pronounced negative influence of stress was observed in BHR despite preserved endothelial function.
authors.affiliation: Institute of Normal and Pathological Physiology, Centre of Excellence for Examination of Regulatory Role of Nitric Oxide in Civilization Diseases, Slovak Academy of Sciences, Sienkiewiczova 1, 813 71 Bratislava, Slovakia.
authors.forename: Peter
authors.identifier: 0000-0001-9757-3283
authors.initials: P
authors.lastname: Slezak
authors.affiliation: Institute of Normal and Pathological Physiology, Centre of Excellence for Examination of Regulatory Role of Nitric Oxide in Civilization Diseases, Slovak Academy of Sciences, Sienkiewiczova 1, 813 71 Bratislava, Slovakia.
authors.forename: Angelika
authors.initials: A
authors.lastname: Puzserova
authors.affiliation: Institute of Normal and Pathological Physiology, Centre of Excellence for Examination of Regulatory Role of Nitric Oxide in Civilization Diseases, Slovak Academy of Sciences, Sienkiewiczova 1, 813 71 Bratislava, Slovakia.
authors.forename: Peter
authors.initials: P
authors.lastname: Balis
authors.affiliation: Institute of Normal and Pathological Physiology, Centre of Excellence for Examination of Regulatory Role of Nitric Oxide in Civilization Diseases, Slovak Academy of Sciences, Sienkiewiczova 1, 813 71 Bratislava, Slovakia.
authors.forename: Natalia
authors.initials: N
authors.lastname: Sestakova
authors.affiliation: Institute of Normal and Pathological Physiology, Centre of Excellence for Examination of Regulatory Role of Nitric Oxide in Civilization Diseases, Slovak Academy of Sciences, Sienkiewiczova 1, 813 71 Bratislava, Slovakia.
authors.forename: Miroslava
authors.identifier: 0000-0001-8748-4279
authors.initials: M
authors.lastname: Majzunova
authors.affiliation: Institute of Normal and Pathological Physiology, Centre of Excellence for Examination of Regulatory Role of Nitric Oxide in Civilization Diseases, Slovak Academy of Sciences, Sienkiewiczova 1, 813 71 Bratislava, Slovakia.
authors.forename: Ima
authors.initials: I
authors.lastname: Dovinova
authors.affiliation: Institute of Normal and Pathological Physiology, Centre of Excellence for Examination of Regulatory Role of Nitric Oxide in Civilization Diseases, Slovak Academy of Sciences, Sienkiewiczova 1, 813 71 Bratislava, Slovakia.
authors.forename: Michal
authors.initials: M
authors.lastname: Kluknavsky
authors.affiliation: Institute of Normal and Pathological Physiology, Centre of Excellence for Examination of Regulatory Role of Nitric Oxide in Civilization Diseases, Slovak Academy of Sciences, Sienkiewiczova 1, 813 71 Bratislava, Slovakia.
authors.forename: Iveta
authors.identifier: 0000-0002-6120-706X
authors.initials: I
authors.lastname: Bernatova
mesh_terms: D000818:Animals; D001794:Blood Pressure; D003441:Crowding; D005260:Female; D005263:Femoral Artery / Q000503:physiopathology*; D005828:Genetics, Population; D005838:Genotype; D006973:Hypertension; D009569:Nitric Oxide / Q000378:metabolism*; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D013045:Species Specificity; D013315:Stress, Psychological / Q000503:physiopathology*; D014661:Vasoconstriction; D014666:Vasomotor System / Q000503:physiopathology*
chemical_list: D009569:Nitric Oxide
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