title: Isolation, purification and antioxidant activities of polysaccharides from Grifola frondosa.
abstract: The crude polysaccharides (GFP) were isolated from the fruiting bodies of Grifola frondosa and purified by DEAE cellulose-52 chromatography and Sephadex G-100 size-exclusion chromatography in that order. Three main fractions, GFP-1, GFP-2 and GFP-3, were obtained through the isolation and purification steps. Then the antioxidant activities of these three fractions were investigated in vitro. The results showed that GFP-1, GFP-2 and GFP-3 possessed significant inhibitory effects on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, hydroxyl radical and superoxide radical; their reducing power, ferrous ions chelating effect and the inhibition ability of the rat liver lipid oxidation where also strong. These results suggest that G. frondosa polysaccharides could be a suitable natural antioxidant and may be the functional foods for humans.
authors.affiliation: Department of Food Quality and Safety, China Pharmaceutical University, 24 Tongjiaxiang, Nanjing 210009, China.
authors.forename: Gui-tang
authors.initials: GT
authors.lastname: Chen
authors.affiliation: Department of Food Quality and Safety, China Pharmaceutical University, 24 Tongjiaxiang, Nanjing 210009, China.
authors.forename: Xue-mei
authors.initials: XM
authors.lastname: Ma
authors.affiliation: Department of Food Quality and Safety, China Pharmaceutical University, 24 Tongjiaxiang, Nanjing 210009, China.
authors.forename: Sheng-to
authors.initials: ST
authors.lastname: Liu
authors.affiliation: Department of Food Quality and Safety, China Pharmaceutical University, 24 Tongjiaxiang, Nanjing 210009, China.
authors.forename: Yan-li
authors.initials: YL
authors.lastname: Liao
authors.affiliation: Chestnut Research and Development Center of Qianxi County, Qianxi, Tang Shan 064300, China.
authors.forename: Guo-qang
authors.initials: GQ
authors.lastname: Zhao
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D001713:Biphenyl Compounds / Q000737:chemistry; D002614:Chelating Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D005292:Ferricyanides / Q000737:chemistry; D005296:Ferrous Compounds / Q000737:chemistry; D048690:Fruiting Bodies, Fungal; D062610:Fungal Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D048691:Grifola; D017665:Hydroxyl Radical / Q000737:chemistry; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D010084:Oxidation-Reduction; D010851:Picrates / Q000737:chemistry; D017207:Rats, Sprague-Dawley; D013481:Superoxides / Q000737:chemistry
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D002614:Chelating Agents; D005292:Ferricyanides; D005296:Ferrous Compounds; D062610:Fungal Polysaccharides; D010851:Picrates; D013481:Superoxides; D017665:Hydroxyl Radical; C004931:1,1-diphenyl-2-picrylhydrazyl; C028033:potassium ferricyanide