title: Vibrio vulnificus phage PV94 is closely related to temperate phages of V. cholerae and other Vibrio species.
abstract: BACKGROUND
  Vibrio vulnificus is an important pathogen which can cause serious infections in humans. Yet, there is limited knowledge on its virulence factors and the question whether temperate phages might be involved in pathogenicity, as is the case with V. cholerae. Thus far, only two phages (SSP002 and VvAW1) infecting V. vulnificus have been genetically characterized. These phages were isolated from the environment and are not related to Vibrio cholerae phages. The lack of information on temperate V. vulnificus phages prompted us to isolate those phages from lysogenic strains and to compare them with phages of other Vibrio species.
  
  
  RESULTS
  In this study the temperate phage PV94 was isolated from a V. vulnificus biotype 1 strain by mitomycin C induction. PV94 is a myovirus whose genome is a linear double-stranded DNA of 33,828 bp with 5'-protruding ends. Sequence analysis of PV94 revealed a modular organization of the genome. The left half of the genome comprising the immunity region and genes for the integrase, terminase and replication proteins shows similarites to V. cholerae kappa phages whereas the right half containing genes for structural proteins is closely related to a prophage residing in V. furnissii NCTC 11218.
  
  
  CONCLUSIONS
  We present the first genomic sequence of a temperate phage isolated from a human V. vulnificus isolate. The sequence analysis of the PV94 genome demonstrates the wide distribution of closely related prophages in various Vibrio species. Moreover, the mosaicism of the PV94 genome indicates a high degree of horizontal genetic exchange within the genus Vibrio, by which V. vulnificus might acquire virulence-associated genes from other species.
authors.affiliation: Federal Institute for Risk Assessment (Bundesinstitut für Risikobewertung), Department of Biological Safety, Berlin, Germany.
authors.forename: Mark
authors.initials: M
authors.lastname: Pryshliak
authors.affiliation: Federal Institute for Risk Assessment (Bundesinstitut für Risikobewertung), Department of Biological Safety, Berlin, Germany.
authors.forename: Jens A
authors.initials: JA
authors.lastname: Hammerl
authors.affiliation: Federal Institute for Risk Assessment (Bundesinstitut für Risikobewertung), Department of Biological Safety, Berlin, Germany.
authors.forename: Jochen
authors.initials: J
authors.lastname: Reetz
authors.affiliation: Federal Institute for Risk Assessment (Bundesinstitut für Risikobewertung), Department of Biological Safety, Berlin, Germany.
authors.forename: Eckhard
authors.initials: E
authors.lastname: Strauch
authors.affiliation: Federal Institute for Risk Assessment (Bundesinstitut für Risikobewertung), Department of Biological Safety, Berlin, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Hertwig
mesh_terms: D001435:Bacteriophages / Q000235:genetics* / Q000302:isolation & purification; D001483:Base Sequence; D016679:Genome, Viral; D006801:Humans; D008242:Lysogeny; D046529:Microscopy, Electron, Transmission; D016685:Mitomycin / Q000737:chemistry; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D039002:Prophages / Q000235:genetics / Q000302:isolation & purification; D014158:Transcription, Genetic; D014733:Vibrio / Q000821:virology*; D014734:Vibrio cholerae / Q000821:virology*; D041261:Vibrio vulnificus / Q000821:virology*; D014774:Virulence
chemical_list: D016685:Mitomycin
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