title: Effect of red clay on diesel bioremediation and soil bacterial community.
abstract: Red clay is a type of soil, the red color of which results from the presence of iron oxide. It is considered an eco-friendly material, with many industrial, cosmetic, and architectural uses. A patented method was applied to red clay in order to change its chemical composition and mineral bioavailability. The resulting product was designated processed red clay. This study evaluates the novel use of red clay and processed red clay as biostimulation agents in diesel-contaminated soils. Diesel biodegradation was enhanced in the presence of red clay and processed red clay by 4.9- and 6.7-fold, respectively, and the number of culturable bacterial cells was correlated with the amount of diesel biodegradation. The growth of Acinetobacter oleivorans DR1, Pseudomonas putida KT2440, and Cupriavidus necator was promoted by both types of red clays. Culture-independent community analysis determined via barcoded pyrosequencing indicated that Nocardioidaceae, Xanthomonadaceae, Pseudomonadaceae, and Caulobacteraceae were enriched by diesel contamination. Bacterial strain isolation from naphthalene- and liquid paraffin-amended media was affiliated with enriched taxa based on 16S rRNA gene sequence identity. We suggest that the biostimulating mechanism of red clay and processed red clay is able to support bacterial growth without apparent selection for specific bacterial species.
authors.affiliation: Department of Environmental Science and Ecological Engineering, Korea University, Seoul, 136-713, Republic of Korea.
authors.forename: Jaejoon
authors.initials: J
authors.lastname: Jung
authors.forename: Sungjong
authors.initials: S
authors.lastname: Choi
authors.forename: Hyerim
authors.initials: H
authors.lastname: Hong
authors.forename: Jung-Suk
authors.initials: JS
authors.lastname: Sung
authors.forename: Woojun
authors.initials: W
authors.lastname: Park
mesh_terms: D000538:Aluminum Silicates; D001419:Bacteria / Q000145:classification / Q000254:growth & development*; D001431:Bacteriological Techniques; D001673:Biodegradation, Environmental; D000077215:Clay; D058893:DNA Barcoding, Taxonomic; D004269:DNA, Bacterial / Q000235:genetics; D005742:Gasoline / Q000382:microbiology*; D059013:Microbial Consortia; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*
chemical_list: D000538:Aluminum Silicates; D004269:DNA, Bacterial; D005742:Gasoline; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; D000077215:Clay
references.citation: Environ Microbiol. 2011 Nov;13(11):3000-9
references.pmid: 21910812
references.citation: Appl Environ Microbiol. 2011 Oct;77(20):7418-24
references.pmid: 21856821
references.citation: Environ Sci Technol. 2006 Apr 15;40(8):2578-85
references.pmid: 16683595
references.citation: Microbiol Rev. 1996 Jun;60(2):342-65
references.pmid: 8801437
references.citation: Environ Pollut. 2005 Jul;136(1):187-95
references.pmid: 15809120
references.citation: Appl Environ Microbiol. 2003 Mar;69(3):1800-9
references.pmid: 12620873
references.citation: J Bacteriol. 2010 Sep;192(18):4794-5
references.pmid: 20639327
references.citation: Nucleic Acids Res. 2009 Jan;37(Database issue):D141-5
references.pmid: 19004872
references.citation: Biotechnol Res Int. 2013;2013:704806
references.pmid: 23864952
references.citation: Sci Total Environ. 2013 Feb 15;445-446:347-55
references.pmid: 23354375
references.citation: J Gen Microbiol. 1966 May;43(2):159-271
references.pmid: 5963505
references.citation: Int J Syst Evol Microbiol. 2012 Mar;62(Pt 3):716-721
references.pmid: 22140171
references.citation: J Environ Biol. 2009 Jan;30(1):145-50
references.pmid: 20112877
references.citation: Mar Pollut Bull. 2013 Dec 15;77(1-2):37-44
references.pmid: 24229785
references.citation: Front Microbiol. 2012 Aug 21;3:290
references.pmid: 22934091
references.citation: Appl Environ Microbiol. 2013 Jan;79(2):619-30
references.pmid: 23144139
references.citation: J Hazard Mater. 2009 Oct 15;170(1):495-505
references.pmid: 19619942
references.citation: Appl Environ Microbiol. 2013 Oct;79(19):5927-35
references.pmid: 23872573
references.citation: Mar Pollut Bull. 2008 Nov;56(11):1855-61
references.pmid: 18799169
references.citation: Appl Biochem Biotechnol. 2008 Dec;151(2-3):638-52
references.pmid: 18592409
references.citation: Mol Biol Evol. 2011 Oct;28(10):2731-9
references.pmid: 21546353
references.citation: Appl Environ Microbiol. 2009 Aug;75(15):5111-20
references.pmid: 19502440
references.citation: Biodegradation. 2011 Apr;22(2):231-41
references.pmid: 20680666
references.citation: Environ Technol. 2012 Sep;33(16-18):1879-93
references.pmid: 23240181
references.citation: ISME J. 2013 Jun;7(6):1200-10
references.pmid: 23389106
references.citation: Microb Ecol. 1986 Mar;12(1):155-72
references.pmid: 24212466
references.citation: PLoS One. 2011 Feb 16;6(2):e17000
references.pmid: 21359220
references.citation: Microb Ecol. 2006 Oct;52(3):523-32
references.pmid: 16944337
references.citation: Appl Environ Microbiol. 2009 Oct;75(19):6258-67
references.pmid: 19684169
references.citation: Appl Biochem Biotechnol. 2009 May;153(1-3):58-66
references.pmid: 19148778