title: Macular pigment optical density measurements by one-wavelength reflection photometry--influence of cataract surgery on the measurement results.
abstract: OBJECTIVE
  The main objective of the present study was the investigation of possible influence of lens opacification on macular pigment optical density (MPOD) measurements.
  
  
  METHODS
  Eighty-six eyes of 64 patients (mean age 73.4 ± 8.3 years) were included in the study. MPOD was prospectively measured using the one-wavelength reflection method (Visucam500, Carl Zeiss Meditec AG) before and after cataract extraction, with implantation of a blue-light filtering intraocular lens (AlconSN60WF). The median of the maximum optical density (MaxOD) and the median of the mean optical density (MeanOD) measurements of macular pigment across the subject group were evaluated.
  
  
  RESULTS
  Statistically significant differences were noticed between pre-operative and post-operative measurements, the absolute values were generally lower after cataract extraction. The following median (lower/upper quartile) differences across the group were determined: MaxOD -33.8 % (-46.2 to -19.1 %), MeanOD -44.0 % (-54.6 to -26.6 %). Larger changes were observed in elderly patients [<70 years of age (n = 25 eyes): MaxOD -13.4 % (-20.5 to 3.6 %), MeanOD -23.6 % (-30.5 to -15.3 %) versus patients ≥70 years (n = 61 eyes) MaxOD -40.5 % (-53.2 to -30.1 %), MeanOD -47.2 % (-57.8 to -40.1 %)] and in patients with progressed stage of cataract. MaxOD for lens opacification grade 1 (n = 9 eyes): -27.4 % (-42.1 to -19.6 %), grade 2 (n = 26 eyes): -35.0 % (-44.2 to -25.3 %), grade 3 (n = 21 eyes): -34.4 % (-45.4 to -11.4 %), grade 4 (n = 25 eyes): -32.6 % (-53.2 to -6.4 %), and grade 5 (n = 5 eyes): -53.5 % (-61.7 to -38.7 %) and MeanOD for cataract stage 1 (n = 9 eyes): -42.6 % (-46.0 to -26.0 %), stage 2 (n = 26 eyes): -44.1 % (-51.8 to -26.2 %), stage 3 (n = 21 eyes): -45.7 % (-54.7 to -24.7 %), stage 4 (n = 25 eyes): -39.5 % (-59.4 to -26.1 %), and stage 5 (n = 5 eyes): -57.0 % (-66.1 to -51.4 %).
  
  
  CONCLUSIONS
  As established by comparison of pre- to post-operative measurements, cataract presented a strong effect on MPOD measured by one-wavelength reflection method. Particular care should therefore be taken when evaluating MPOD using this method in elderly patients with progressed stage of cataract. Future optimization of correcting parameters of scattered light and consideration of cataract influence may allow more precise evaluation of MPOD.
authors.affiliation: Department of Ophthalmology, University Hospital Leipzig, Liebigstrasse 10-14, 04103, Leipzig, Germany.
authors.forename: Bogdana
authors.initials: B
authors.lastname: Komar
authors.forename: Franziska Georgia
authors.initials: FG
authors.lastname: Rauscher
authors.forename: Renate
authors.initials: R
authors.lastname: Wiedemann
authors.forename: Jens
authors.initials: J
authors.lastname: Dawczynski
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D002386:Cataract / Q000378:metabolism*; D003720:Densitometry; D005260:Female; D006801:Humans; D019654:Lens Implantation, Intraocular; D014975:Lutein / Q000378:metabolism; D008266:Macula Lutea / Q000378:metabolism*; D065348:Macular Pigment / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D018918:Phacoemulsification; D010783:Photometry / Q000379:methods*; D011446:Prospective Studies; D065146:Zeaxanthins / Q000378:metabolism
chemical_list: D065348:Macular Pigment; D065146:Zeaxanthins; D014975:Lutein
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