title: Trim9 regulates activity-dependent fine-scale topography in Drosophila.
abstract: Topographic projection of afferent terminals into 2D maps in the CNS is a general strategy used by the nervous system to encode the locations of sensory stimuli. In vertebrates, it is known that although guidance cues are critical for establishing a coarse topographic map, neural activity directs fine-scale topography between adjacent afferent terminals [1-4]. However, the molecular mechanism underlying activity-dependent regulation of fine-scale topography is poorly understood. Molecular analysis of the spatial relationship between adjacent afferent terminals requires reliable localization of the presynaptic terminals of single neurons as well as genetic manipulations with single-cell resolution in vivo. Although both requirements can potentially be met in Drosophila melanogaster [5, 6], no activity-dependent topographic system has been identified in flies [7]. Here we report a topographic system that is shaped by neuronal activity in Drosophila. With this system, we found that topographic separation of the presynaptic terminals of adjacent nociceptive neurons requires different levels of Trim9, an evolutionarily conserved signaling molecule [8-11]. Neural activity regulates Trim9 protein levels to direct fine-scale topography of sensory afferents. This study offers both a novel mechanism by which neural activity directs fine-scale topography of axon terminals and a new system to study this process at single-neuron resolution.
authors.affiliation: Life Sciences Institute and Department of Cell and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA; Department of Physiology, Basic Medical School, Jiamusi University, Jiamusi, Heilongjiang 154007, China.
authors.forename: Limin
authors.initials: L
authors.lastname: Yang
authors.affiliation: Department of Physiology, Basic Medical School, Jiamusi University, Jiamusi, Heilongjiang 154007, China.
authors.forename: Ruonan
authors.initials: R
authors.lastname: Li
authors.affiliation: Life Sciences Institute and Department of Cell and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA.
authors.forename: Takuya
authors.initials: T
authors.lastname: Kaneko
authors.affiliation: Department of Neurobiology, University of Massachusetts, Worcester, MA 01605, USA.
authors.forename: Kendra
authors.initials: K
authors.lastname: Takle
authors.affiliation: Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Rei K
authors.initials: RK
authors.lastname: Morikawa
authors.affiliation: Life Sciences Institute and Department of Cell and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA.
authors.forename: Laura
authors.initials: L
authors.lastname: Essex
authors.affiliation: Life Sciences Institute and Department of Cell and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Computer Science, Northern Illinois University, DeKalb, IL 60115, USA.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Emoto
authors.affiliation: Department of Neurobiology, University of Massachusetts, Worcester, MA 01605, USA.
authors.forename: Yang
authors.initials: Y
authors.lastname: Xiang
authors.affiliation: Life Sciences Institute and Department of Cell and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA. Electronic address: bingye@umich.edu.
authors.forename: Bing
authors.initials: B
authors.lastname: Ye
mesh_terms: D000344:Afferent Pathways / Q000502:physiology*; D000818:Animals; D029721:Drosophila Proteins / Q000235:genetics*; D004331:Drosophila melanogaster / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000235:genetics*; D009619:Nociceptors / Q000378:metabolism; D017729:Presynaptic Terminals / Q000502:physiology*; D019371:Topography, Medical; D000070584:Tripartite Motif Proteins; D044767:Ubiquitin-Protein Ligases / Q000235:genetics*
chemical_list: D029721:Drosophila Proteins; D009419:Nerve Tissue Proteins; D000070584:Tripartite Motif Proteins; C000594403:TRIM9 protein, Drosophila; D044767:Ubiquitin-Protein Ligases
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