title: Multicompartment lipid cubic nanoparticles with high protein upload: millisecond dynamics of formation.
abstract: Membrane shapes, produced by dynamically assembled lipid/protein architectures, are crucial for both physiological functions and the design of therapeutic nanotechnologies. Here we investigate the dynamics of lipid membrane-neurotrophic BDNF protein complexes formation and ordering in nanoparticles, with the purpose of innovation in nanostructure-based neuroprotection and biomimetic nanoarchitectonics. The kinetic pathway of membrane states associated with rapidly occurring nonequilibrium self-assembled lipid/protein nanoarchitectures was determined by millisecond time-resolved small-angle X-ray scattering (SAXS) at high resolution. The neurotrophin binding and millisecond trafficking along the flexible membranes induced an unusual overlay of channel-network architectures including two coexisting cubic lattices epitaxially connected to lamellar membrane stacks. These time-resolved membrane processes, involving intercalation of discrete stiff proteins in continuous soft membranes, evidence stepwise curvature control mechanisms. The obtained three-phase liquid-crystalline nanoparticles of neurotrophic composition put forward important advancements in multicompartment soft-matter nanostructure design.
authors.affiliation: Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic , Heyrovsky Sq. 2, CZ-16206 Prague, Czech Republic.
authors.forename: Borislav
authors.initials: B
authors.lastname: Angelov
authors.forename: Angelina
authors.initials: A
authors.lastname: Angelova
authors.forename: Sergey K
authors.initials: SK
authors.lastname: Filippov
authors.forename: Markus
authors.initials: M
authors.lastname: Drechsler
authors.forename: Petr
authors.initials: P
authors.lastname: Štěpánek
authors.forename: Sylviane
authors.initials: S
authors.lastname: Lesieur
mesh_terms: D032701:Biomimetics; D019208:Brain-Derived Neurotrophic Factor / Q000737:chemistry; D006801:Humans; D007700:Kinetics; D008051:Lipid Bilayers / Q000737:chemistry; D008055:Lipids / Q000737:chemistry*; D050866:Liquid Crystals; D008567:Membranes, Artificial; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods*; D009414:Nerve Growth Factors / Q000737:chemistry*; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry; D053838:Scattering, Small Angle
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D008051:Lipid Bilayers; D008055:Lipids; D008567:Membranes, Artificial; D009414:Nerve Growth Factors; D011506:Proteins; D011994:Recombinant Proteins; C579455:BDNF protein, human