title: Bisbenzylisoquinoline and hasubanane alkaloids from Stephania abyssinica (Dillon & A. Rich) (Menispermeceae).
abstract: Two bisbenzylisoquinoline and one hasubanane alkaloids: (-)-pseudocurine (1), (-)-pseudoisocurine (2) and (-)-10-oxoaknadinine (3), were isolated from leaf extract of Stephania abyssinica, a plant used in traditional medicine in South Nyanza region of Kenya. They were characterized using 1D ((1)H, (13)C and DEPT) and 2D (COSY, NOESY, HMQC and HMBC) NMR techniques. (-)-Pseudocurine (1) and (-)-pseudoisocurine (2) exhibited strong to moderate anti-plasmodial activity while (-)-10-oxoaknadinine (3) showed moderate to mild activity.
authors.affiliation: Department of Chemistry, Kenyatta University, P.O. Box 43844, Nairobi 00100, Kenya; Department of Chemical Science and Technology, Technical University of Kenya, P.O Box 52428, Nairobi 00200, Kenya. Electronic address: oruth2002@yahoo.com.
authors.forename: Ruth A
authors.initials: RA
authors.lastname: Omole
authors.affiliation: Centre for Traditional Medicine & Drug Research, Kenya Medical Research Institute (KEMRI), P.O Box 54840, Nairobi 00200, Kenya. Electronic address: jgathirwa@gmail.com.
authors.forename: Jeremiah
authors.initials: J
authors.lastname: Gathirwa
authors.affiliation: Department of Emerging Infectious Diseases-Global Emerging Infections Surveillance and Response System (DEID-GEIS) Program, United States Army Medical Research Unit-Kenya (USAMRU-K), Kenya Medical Research Institute (KEMRI)-Walter Reed Project, P.O. Box 54, 40100 Kisumu, Kenya. Electronic address: hoseaakala@yahoo.com.
authors.forename: Hosea
authors.initials: H
authors.lastname: Akala
authors.affiliation: Department of Traditional Medicine Research, National Institute for Medical Research (NIMR), P.O. Box 9653, Dar es Salaam, Tanzania. Electronic address: malebo@hotmail.com.
authors.forename: Hamisi M
authors.initials: HM
authors.lastname: Malebo
authors.affiliation: Department of Chemistry, Kenyatta University, P.O. Box 43844, Nairobi 00100, Kenya. Electronic address: machochoalex@hotmail.com.
authors.forename: Alex K
authors.initials: AK
authors.lastname: Machocho
authors.affiliation: Department of Chemistry, Kenyatta University, P.O. Box 43844, Nairobi 00100, Kenya; Behavioural & Chemical Ecology Department, International Centre for Insect Physiology & Ecology (ICIPE), P.O. Box 30772, Nairobi 00100, Kenya. Electronic address: ahmedhassanali786@gmail.com.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Hassanali
authors.affiliation: Department of Chemistry, Kenyatta University, P.O. Box 43844, Nairobi 00100, Kenya; Office of the Vice Chancellor, Kyambogo University, P.O. Box 1, Kyambogo, Kampala, Uganda. Electronic address: indiege@yahoo.com.
authors.forename: Isaiah O
authors.initials: IO
authors.lastname: Ndiege
mesh_terms: D000470:Alkaloids / Q000737:chemistry* / Q000494:pharmacology*; D000962:Antimalarials / Q000737:chemistry* / Q000494:pharmacology*; D044182:Benzylisoquinolines / Q000737:chemistry* / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D010963:Plasmodium falciparum / Q000187:drug effects; D031607:Stephania / Q000737:chemistry*
chemical_list: D000470:Alkaloids; D000962:Antimalarials; D044182:Benzylisoquinolines
keywords: (−)-10-oxoaknadinine anti-plasmodial activity; (−)-Pseudocurine and (−)-pseudoisocurine; Alkaloids; Bisbenzylisoquinoline; Hasubanane; Menispermerceae; Stephania abyssinica