title: Hypoxia-induced endothelial damage and microthrombosis in myocardial vessels of newborn landrace/large white piglets.
abstract: OBJECTIVE
  Evaluating the presence of endothelial changes in myocardial vessels in an experimental model of hypoxia and resuscitation in newborn piglets.
  
  
  METHODS
  Fifty male Landrace/Large White neonatal piglets were studied: ten of them were allocated in group A (control group, SHAM-operated). In group B (forty animals, experimental group) normocapnic hypoxia was induced by decreasing inspired concentration of O2 to 6%-8%. When the animals developed bradycardia or severe hypotension, reoxygenation was initiated. The animals of group B were allocated in 4 subgroups of 10, according to the concentration of O2 they were resuscitated with (groups 1, 2, 3, and 4 received 18%, 21%, 40%, and 100% O2, resp.).
  
  
  RESULTS
  Control group animals did not show any significant endothelial lesions. Contrarily, endothelial lesions were detected in all experimental group cases. When these lesions were analyzed in the different heart zones, no significant difference in their incidence was observed; analyzing the frequency in the animals of the 4 subgroups, only microthrombosis showed a higher frequency in animals in groups 4 and 3.
  
  
  CONCLUSIONS
  Endothelial damage represents a diffuse pathological feature in the myocardial vessels of piglets subjected to normocapnic hypoxia and resuscitation suggesting a possible role of hyperoxygenation in aggravating endothelial damage.
authors.affiliation: Department of Pathology, University of Cagliari, Via Ospedale 46, 09100 Cagliari, Italy.
authors.forename: Armando
authors.identifier: 0000-0003-3768-0890
authors.initials: A
authors.lastname: Faa
authors.affiliation: Medical School, National and Kapodistrian University of Athens, 100 Athens, Greece.
authors.forename: Theodoros
authors.identifier: 0000-0003-3670-9305
authors.initials: T
authors.lastname: Xanthos
authors.affiliation: Department of Paediatrics, Neonatal Intensive Care Unit, Puericulture Institute and Neonatal Section, University of Cagliari, 09100 Cagliari, Italy.
authors.forename: Vassilios
authors.initials: V
authors.lastname: Fanos
authors.affiliation: Department of Pathology, University of Cagliari, Via Ospedale 46, 09100 Cagliari, Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: Fanni
authors.affiliation: Department of Pathology, University of Cagliari, Via Ospedale 46, 09100 Cagliari, Italy.
authors.forename: Clara
authors.initials: C
authors.lastname: Gerosa
authors.affiliation: Department of Pathology, University of Cagliari, Via Ospedale 46, 09100 Cagliari, Italy.
authors.forename: Pietro
authors.initials: P
authors.lastname: Pampaloni
authors.affiliation: Department of Pathology, University of Cagliari, Via Ospedale 46, 09100 Cagliari, Italy.
authors.forename: Maria Elena
authors.initials: ME
authors.lastname: Pais
authors.affiliation: Department of Pathology, University of Cagliari, Via Ospedale 46, 09100 Cagliari, Italy.
authors.forename: Gavino
authors.initials: G
authors.lastname: Faa
authors.affiliation: 2nd Department of Obstetrics and Gynecology, Neonatal Division, Medical School, National and Kapodistrian University of Athens, 100 Athens, Greece.
authors.forename: Nicoletta
authors.identifier: 0000-0001-8021-6191
authors.initials: N
authors.lastname: Iacovidou
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D003328:Coronary Thrombosis / Q000209:etiology / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D003331:Coronary Vessels / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D004730:Endothelium, Vascular / Q000293:injuries / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D000860:Hypoxia / Q000150:complications / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D008297:Male; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D013552:Swine
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