title: Evaluation of HemogloBind™ treatment for preparation of samples for cholinesterase analysis.
abstract: Acetylcholine is an essential neurotransmitter found throughout the nervous system. Its action on postsynaptic receptors is regulated through hydrolysis by various carboxylesterases, especially cholinesterases (ChEs). The acute toxicity of organophosphate (OP) compounds is directly linked to their action as inhibitors of ChE. One widely used assay for evaluating ChE activity is a spectrophotometric method developed by Ellmanet al.When the enzyme source is from tissues or, in particular, blood, hemoglobin displays a spectrophotometric peak at the same wavelength used to analyze cholinergic activity. This creates a substantial background that interferes with the Ellman's assay and must be overcome in order to accurately monitor cholinesterase activity. Herein, we directly compare blood processing methods: classical method (1.67 ± 0.30 U/mL) and HemogloBind™ treatment (1.51 ± 0.17 U/mL), and clearly demonstrate that pretreatment of blood samples with Hemoglobind™ is both a sufficient and rapid sample preparation method for the assessment of ChE activity using the Ellman's method.
authors.affiliation: Battelle Biomedical Research Center, Battelle Memorial Institute, Columbus, USA.
authors.forename: Kevin G
authors.initials: KG
authors.lastname: McGarry
authors.affiliation: Battelle Biomedical Research Center, Battelle Memorial Institute, Columbus, USA.
authors.forename: Ryan A
authors.initials: RA
authors.lastname: Bartlett
authors.affiliation: Battelle Biomedical Research Center, Battelle Memorial Institute, Columbus, USA.
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Machesky
authors.affiliation: Battelle Biomedical Research Center, Battelle Memorial Institute, Columbus, USA.
authors.forename: Thomas H
authors.initials: TH
authors.lastname: Snider
authors.affiliation: Battelle Biomedical Research Center, Battelle Memorial Institute, Columbus, USA.
authors.forename: Robert A
authors.initials: RA
authors.lastname: Moyer
authors.affiliation: National Institutes of Health/National Institute of Neurological Disorders and Stroke, Bethesda, USA.
authors.forename: David T
authors.initials: DT
authors.lastname: Yeung
authors.affiliation: Battelle Biomedical Research Center, Battelle Memorial Institute, Columbus, USA.
authors.forename: Matthew K
authors.initials: MK
authors.lastname: Brittain
keywords: Acetylcholinesterase; Cholinesterase; HemogloBind™; Hemoglobin; Sample Preparation
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