title: Identification of novel bovine group A rotavirus G15P[14] strain from epizootic diarrhea of adult cows by de novo sequencing using a next-generation sequencer.
abstract: There are few reports describing diarrhea of adult cattle caused by group A rotaviruses. Here, we report the identification of a novel bovine group A rotavirus from diarrhea of adult cows. A group A rotavirus was detected from an epizootic outbreak of diarrhea in adult cows with a decrease in milk production in Japan in 2013. The comprehensive genomic analyses from fecal samples by viral metagenomics using a next-generation sequencer revealed that it had an unreported genotype combination G15P[14]. The genome constellation of this strain, namely, RVA/Cow-wt/JPN/Tottori-SG/2013/G15P[14] was G15-P[14]-I2-R2-C2-M2-A3-N2-T6-E2-H3 representing VP7-VP4-VP6-VP1-VP2-VP3-NSP1-NSP2-NSP3-NSP4-NSP5, respectively. Each gene segment of Tottori-SG was most closely related to Japanese bovine group A rotaviruses suggesting that Tottori-SG might have derived from multiple reassortment events from group A rotavirus strains circulating among Japanese cattle. No other diarrhea pathogen of adult cattle was detected by routine diagnosis and metagenomics. Viral metagenomics, using a next-generation sequencer, is useful to characterize group A rotaviruses from fecal samples and offers unbiased comprehensive investigations of pathogen.
authors.affiliation: Kurayoshi Livestock Hygiene Service Center, Kurayoshi, Japan.
authors.forename: Tsuneyuki
authors.initials: T
authors.lastname: Masuda
authors.affiliation: Research and Education Center for Prevention of Global Infectious Disease of Animal, Tokyo University of Agriculture and Technology, Fuchu-shi, Japan. Electronic address: m-nagai@cc.tuat.ac.jp.
authors.forename: Makoto
authors.initials: M
authors.lastname: Nagai
authors.affiliation: Kurayoshi Livestock Hygiene Service Center, Kurayoshi, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yamasato
authors.affiliation: Research and Education Center for Prevention of Global Infectious Disease of Animal, Tokyo University of Agriculture and Technology, Fuchu-shi, Japan.
authors.forename: Shinobu
authors.initials: S
authors.lastname: Tsuchiaka
authors.affiliation: Research and Education Center for Prevention of Global Infectious Disease of Animal, Tokyo University of Agriculture and Technology, Fuchu-shi, Japan.
authors.forename: Sachiko
authors.initials: S
authors.lastname: Okazaki
authors.affiliation: Research and Education Center for Prevention of Global Infectious Disease of Animal, Tokyo University of Agriculture and Technology, Fuchu-shi, Japan.
authors.forename: Yukie
authors.initials: Y
authors.lastname: Katayama
authors.affiliation: Research and Education Center for Prevention of Global Infectious Disease of Animal, Tokyo University of Agriculture and Technology, Fuchu-shi, Japan.
authors.forename: Mami
authors.initials: M
authors.lastname: Oba
authors.affiliation: Research and Education Center for Prevention of Global Infectious Disease of Animal, Tokyo University of Agriculture and Technology, Fuchu-shi, Japan.
authors.forename: Naomi
authors.initials: N
authors.lastname: Nishiura
authors.affiliation: Research and Education Center for Prevention of Global Infectious Disease of Animal, Tokyo University of Agriculture and Technology, Fuchu-shi, Japan.
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Sassa
authors.affiliation: Research and Education Center for Prevention of Global Infectious Disease of Animal, Tokyo University of Agriculture and Technology, Fuchu-shi, Japan.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Omatsu
authors.affiliation: Research and Education Center for Prevention of Global Infectious Disease of Animal, Tokyo University of Agriculture and Technology, Fuchu-shi, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Furuya
authors.affiliation: Research and Education Center for Prevention of Global Infectious Disease of Animal, Tokyo University of Agriculture and Technology, Fuchu-shi, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Koyama
authors.affiliation: Research and Education Center for Prevention of Global Infectious Disease of Animal, Tokyo University of Agriculture and Technology, Fuchu-shi, Japan.
authors.forename: Junsuke
authors.initials: J
authors.lastname: Shirai
authors.affiliation: Department of Virology and Parasitology, School of Medicine, Fujita Health University, Toyoake, Japan.
authors.forename: Koki
authors.initials: K
authors.lastname: Taniguchi
authors.affiliation: Department of Virology II, National Institute of Infectious Diseases, Musashimurayama, Japan.
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Fujii
authors.affiliation: Department of Virology II, National Institute of Infectious Diseases, Musashimurayama, Japan.
authors.forename: Reiko
authors.initials: R
authors.lastname: Todaka
authors.affiliation: Department of Virology II, National Institute of Infectious Diseases, Musashimurayama, Japan.
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Katayama
authors.affiliation: Research and Education Center for Prevention of Global Infectious Disease of Animal, Tokyo University of Agriculture and Technology, Fuchu-shi, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Mizutani
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000821:virology*; D003967:Diarrhea / Q000662:veterinary* / Q000821:virology; D005243:Feces / Q000821:virology*; D005260:Female; D016679:Genome, Viral / Q000235:genetics; D005838:Genotype; D007564:Japan; D008969:Molecular Sequence Data; D010802:Phylogeny; D012401:Rotavirus / Q000145:classification* / Q000235:genetics*; D012400:Rotavirus Infections / Q000662:veterinary* / Q000821:virology; D017422:Sequence Analysis, DNA / Q000295:instrumentation; D017385:Sequence Homology
keywords: Adult cow diarrhea; Japan; Novel genotype; Rotavirus; Viral metagenomics
references.citation: J Vet Med Sci. 2013 Dec 30;75(12):1651-5
references.pmid: 23912876
references.citation: BMC Genomics. 2008 Jan 07;9:5
references.pmid: 18179705
references.citation: J Infect Dis. 2002 May 15;185(10):1502-5
references.pmid: 11992287
references.citation: Virus Genes. 2008 Oct;37(2):250-65
references.pmid: 18661221
references.citation: Virus Genes. 2008 Oct;37(2):241-9
references.pmid: 18679786
references.citation: Arch Virol. 1997;142(5):1059-69
references.pmid: 9191871
references.citation: Arch Virol. 2013 May;158(5):1021-30
references.pmid: 23263646
references.citation: Epidemiol Infect. 2012 Jun;140(6):1013-7
references.pmid: 21943834
references.citation: J Virol. 2009 Apr;83(7):2917-29
references.pmid: 19153225
references.citation: Lancet Infect Dis. 2004 Feb;4(2):91-9
references.pmid: 14871633
references.citation: Emerg Infect Dis. 2013 Aug;19(8):1324-7
references.pmid: 23876354
references.citation: Arch Virol. 1996;141(3-4):705-13
references.pmid: 8645106
references.citation: Nature. 2012 Apr 15;485(7397):256-9
references.pmid: 22504179
references.citation: Arch Virol. 2001;146(3):557-70
references.pmid: 11338390
references.citation: Arch Virol. 2004 Dec;149(12):2465-71
references.pmid: 15449134
references.citation: J Vet Diagn Invest. 1994 Jul;6(3):302-7
references.pmid: 7948199
references.citation: Virology. 1994 Apr;200(1):66-71
references.pmid: 8128639
references.citation: Virus Res. 2009 Sep;144(1-2):250-7
references.pmid: 19464329
references.citation: J Virol. 2006 Apr;80(8):3801-10
references.pmid: 16571797
references.citation: Arch Virol. 1999;144(5):1027-34
references.pmid: 10416384
references.citation: Vet Res Commun. 2009 Jan;33(1):1-23
references.pmid: 18622713
references.citation: J Vet Diagn Invest. 2003 May;15(3):205-12
references.pmid: 12735342
references.citation: BMC Genomics. 2013 Jul 04;14:444
references.pmid: 23822119
references.citation: Arch Virol. 2011 Aug;156(8):1397-413
references.pmid: 21597953
references.citation: J Clin Microbiol. 2007 Aug;45(8):2751-3
references.pmid: 17537935
references.citation: Virology. 1993 Aug;195(2):766-71
references.pmid: 8393244
references.citation: Mol Biol Evol. 2011 Oct;28(10):2731-9
references.pmid: 21546353
references.citation: PLoS One. 2013 Jun 10;8(6):e66129
references.pmid: 23762474
references.citation: Virus Res. 2004 Sep 15;105(1):101-6
references.pmid: 15325086
references.citation: Arch Virol. 1999;144(1):167-75
references.pmid: 10076517
references.citation: J Clin Microbiol. 1997 May;35(5):1266-8
references.pmid: 9114422
references.citation: Arch Virol. 1994;136(3-4):309-23
references.pmid: 8031236
references.citation: J Virol. 2010 Feb;84(4):2013-26
references.pmid: 19939934
references.citation: J Virol. 2012 Sep;86(18):9899-910
references.pmid: 22761376
references.citation: Nat Methods. 2008 Jan;5(1):16-8
references.pmid: 18165802
references.citation: Vet Microbiol. 2006 Nov 26;118(1-2):1-11
references.pmid: 16982159
references.citation: Vet Microbiol. 2011 Mar 24;148(2-4):384-8
references.pmid: 20880637
references.citation: Vet Microbiol. 2007 Jul 20;123(1-3):217-24
references.pmid: 17368763
references.citation: Vet Microbiol. 1996 Feb;48(3-4):367-72
references.pmid: 9054132
references.citation: Virology. 2000 Oct 10;276(1):104-13
references.pmid: 11021999
references.citation: J Gen Virol. 2011 Apr;92(Pt 4):952-60
references.pmid: 21228131
references.citation: BMC Microbiol. 2009 Nov 23;9:238
references.pmid: 19930627
references.citation: Vet Microbiol. 2013 Aug 30;165(3-4):190-9
references.pmid: 23642647