title: Genetic variant rs1058240 at the microRNA-binding site in the GATA3 gene may regulate its mRNA expression.
abstract: The GATA binding protein 3 (GATA3) is a member of a family of 6 GATA dual zinc finger transcription factors (GATA1-6), which are required for the development and morphogenesis of the mammary gland. GATA3 is considered to play a dual role in oncogenesis and cancer development, whereas somaticGATA3mutations have been reported in breast cancer. Variants of theGATA3genetic 3' untranslated region (3'UTR) microRNA (miRNA) binding sites have been associated with breast cancer risk. However, the roles of genetic variants in theGATA3gene 3'UTR and its post-transcriptional regulation have not been fully elucidated. We discovered that rs1058240 in theGATA33'UTR displayed potential miRNA binding sites and this variant was found to be significantly associated withGATA3mRNA expression (P=2.36E-07), suggesting that rs1058240 may be a putative variant mediating the post-transcriptional regulation of theGATA3target gene. Further studies investigating the regulatory mechanism ofGATA3transcriptional activity are required to design novel strategies against breast cancer cell growth and differentiation.
authors.affiliation: Department of Medical Oncology, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, P.R. China.
authors.forename: Fang
authors.initials: F
authors.lastname: Yang
authors.affiliation: Department of Medical Oncology, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, P.R. China.
authors.forename: Fenxia
authors.initials: F
authors.lastname: Chen
authors.affiliation: Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, P.R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Gu
authors.affiliation: Department of Medical Oncology, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, P.R. China.
authors.forename: Wenwen
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Medical Oncology, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, P.R. China.
authors.forename: Jiayan
authors.initials: J
authors.lastname: Luo
authors.affiliation: Department of Medical Oncology, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, P.R. China.
authors.forename: Xiaoxiang
authors.initials: X
authors.lastname: Guan
keywords: GATA3; breast cancer; genetic polymorphisms; microRNA
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