title: The cell-engineered construct of cartilage on the basis of biopolymer hydrogel matrix and human adipose tissue-derived mesenchymal stromal cells (in vitro study).
abstract: The study results of in vitro formation of tissue-engineered cartilage construct on the basis of cell-engineered construct composed of biopolymer hydrogel matrix and human adipose tissue-derived mesenchymal stromal cells (hADSCs) are presented. It was revealed that hADSCs in biopolymer hydrogel matrix Sphero®GEL under chondrogenic conditions generate three-dimensional structures and produce cartilaginous extracellular matrix components: collagen type II and glycosaminoglycans.
authors.affiliation: Department of Biomedical Technologies and Tissue Engineering, Laboratory of Tissue Engineering and Delivery Systems, Academician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation, Shchukinskaya 1, Moscow, Russia, 123182; Institute of Biomedical Research and Technology (ANO), B.Tishinsky, 43/20, Building 2, Moscow, Russia, 123557.
authors.forename: Valentina A
authors.initials: VA
authors.lastname: Surguchenko
authors.forename: Anna S
authors.initials: AS
authors.lastname: Ponomareva
authors.forename: Ljudmila A
authors.initials: LA
authors.lastname: Kirsanova
authors.forename: Nikolaj N
authors.initials: NN
authors.lastname: Skaleckij
authors.forename: Viktor I
authors.initials: VI
authors.lastname: Sevastianov
mesh_terms: D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism*; D002356:Cartilage / Q000737:chemistry*; D002454:Cell Differentiation; D060846:Cell Engineering; D020219:Chondrogenesis; D005109:Extracellular Matrix / Q000378:metabolism; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*
chemical_list: D020100:Hydrogels
keywords: biopolymer matrix; chondrogenic differentiation; heterogeneous hydrogel; human adipose tissue-derived mesenchymal stromal cells; tissue-engineered construct