title: Nanofibrous mats layer-by-layer assembled by HTCC/layered silicate composites with in vitro antitumor activity against SMMC-7721 cells.
abstract: Organic rectorite (OREC) was used to prepare the intercalated nanocomposites with N-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride (HTCC), and then the immobilization of the positively charged HTCC-OREC nanocomposites and the negatively charged sodium alginate (ALG) on cellulose nanofibrous mats was performed through layer-by-layer (LBL) technique. Fiber diameter distribution results from Field Emission Scanning Electron Microscopy (FE-SEM) images showed that the average fiber diameter of (HTCC-OREC/ALG)(n) films coating obviously increased from 433 to 608 nm. Moreover, X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD) results further confirmed the interaction between HTCC and OREC and their successful immobilization on cellulose template. MTT assay indicated that the prepared nanofibrous mats exhibited strong inhibitory activity against human hepatocellular carcinoma cells (SMMC-7721) but a little cytotoxic effect on human Chang liver (CCL-13) cells. Furthermore, the experimental results from FE-SEM and Inverted Fluorescence Microscope of SMMC-7721 cells cultured on LBL structured nanofibrous mats demonstrated the significant antitumor activity of prepared samples. The developed approach to immobilize nanocomposites onto polymer nanofibers with controllable thickness may also be utilized to tumor therapy.
authors.forename: Rong
authors.initials: R
authors.lastname: Huang
authors.forename: Xue
authors.initials: X
authors.lastname: Zhou
authors.forename: Xinqin
authors.initials: X
authors.lastname: Liu
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhang
authors.forename: Huan'guang
authors.initials: H
authors.lastname: Jin
authors.forename: Xiaowen
authors.initials: X
authors.lastname: Shi
authors.forename: Wenjing
authors.initials: W
authors.lastname: Luo
authors.forename: Hongbing
authors.initials: H
authors.lastname: Deng
mesh_terms: D000538:Aluminum Silicates / Q000737:chemistry / Q000627:therapeutic use; D000970:Antineoplastic Agents / Q000737:chemistry / Q000627:therapeutic use*; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy* / Q000473:pathology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000031:analogs & derivatives* / Q000737:chemistry / Q000627:therapeutic use; D004353:Drug Evaluation, Preclinical; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy* / Q000473:pathology; D008903:Minerals / Q000737:chemistry / Q000627:therapeutic use; D053761:Nanocomposites / Q000737:chemistry / Q000627:therapeutic use / Q000648:ultrastructure; D057139:Nanofibers / Q000737:chemistry* / Q000627:therapeutic use* / Q000648:ultrastructure; D058105:Polymerization; D000644:Quaternary Ammonium Compounds / Q000737:chemistry* / Q000627:therapeutic use; D017640:Silicates / Q000737:chemistry* / Q000627:therapeutic use
chemical_list: D000538:Aluminum Silicates; D000970:Antineoplastic Agents; D008903:Minerals; C550160:N-(2-hydroxypropyl)-3-trimethylammonium chitosan; D000644:Quaternary Ammonium Compounds; D017640:Silicates; C546376:rectorite; D048271:Chitosan