title: Regulation of spermatogenesis by small non-coding RNAs: role of the germ granule.
abstract: The spermatogenic process relays in highly regulated gene expression mechanisms at the transcriptional and post-transcriptional levels to generate the male gamete that is needed for the perpetuation of the species. Small non-coding RNA pathways have been determined to participate in the post-transcriptional regulatory processes of germ cells. The most important sncRNA molecules that are critically involved in spermatogenesis belong to the miRNA and piRNAs pathways as illustrated by animal models where ablation of specific protein components displays male infertility. Several elements of these regulatory pathways have been found in the nuage or germ granule, a non-membranous cytoplasmatic structure that can be seen in spermatocytes and spermatids. This notion suggests that germ granules may act as organizer centers for silencing pathways in the germline. In general, miRNAs regulate spermatogenesis through targeting and down-regulation of specific transcripts to eventually promote sperm development. However, piRNAs are powerful repressors of transposon elements expression in the spermatogenic process. Here we describe the suggested functions that miRNA and piRNAs pathways execute in the regulation of spermatogenesis and include some recent studies in the field. Despite major strides on the detailed molecular mechanisms of sncRNAs in relation to spermatogenesis, there is plenty to discover on this fascinating regulatory program.
authors.affiliation: Center for Epigenetics and Metabolism School of Medicine, Department of Biological Chemistry, University of California, Irvine, United States.
authors.forename: Sara
authors.initials: S
authors.lastname: de Mateo
authors.affiliation: Center for Epigenetics and Metabolism School of Medicine, Department of Biological Chemistry, University of California, Irvine, United States. Electronic address: psc@uci.edu.
authors.forename: Paolo
authors.initials: P
authors.lastname: Sassone-Corsi
mesh_terms: D053687:Adult Stem Cells / Q000166:cytology*; D000818:Animals; D017209:Apoptosis / Q000235:genetics; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D006801:Humans; D008297:Male; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D034741:RNA, Small Interfering / Q000235:genetics*; D013087:Spermatids / Q000166:cytology; D013090:Spermatocytes / Q000166:cytology; D013091:Spermatogenesis / Q000502:physiology*; D013737:Testis / Q000166:cytology / Q000196:embryology
chemical_list: D035683:MicroRNAs; D034741:RNA, Small Interfering
keywords: Epigenetics; Germ cells; Germ granules; Post-transcriptional regulation; RBP, RNA binding proteins; Spermatogenesis; miRNAs; piRNAs; sncRNAs
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