title: Ultrasmall superparamagnetic iron oxide nanoparticle prelabelling of human neural precursor cells.
abstract: Stem cells prelabelled with iron oxide nanoparticles can be visualised using magnetic resonance imaging (MRI). This technique allows for noninvasive long-term monitoring of migration, integration and stem cell fate following transplantation into living animals. In order to determine biocompatibility, the present study investigated the biological impact of introducing ultrasmall superparamagnetic iron oxide nanoparticles (USPIOs) into primary human fetal neural precursor cells (hNPCs) in vitro. USPIOs with a mean diameter of 10-15 nm maghemite iron oxide core were sterically stabilised by 95% methoxy-poly(ethylene glycol) (MPEG) and either 5% cationic (NH2) end-functionalised, or 5% Rhodamine B end-functionalised, polyacrylamide. The stabilising polymer diblocks were synthesised by reversible addition-fragmentation chain transfer (RAFT) polymerisation. Upon loading, cellular viability, total iron capacity, differentiation, average distance of migration and changes in intracellular calcium ion concentration were measured to determine optimal loading conditions. Taken together we demonstrate that prelabelling of hNPCs with USPIOs has no significant detrimental effect on cell biology and that USPIOs, when utilised at an optimised dosage, are an effective means of noninvasively tracking prelabelled hNPCs.
authors.affiliation: Department of Anatomy and Histology, Bosch Institute, Sydney Medical School, University of Sydney, NSW 2006, Australia.
authors.forename: Steven S
authors.initials: SS
authors.lastname: Eamegdool
authors.affiliation: Department of Anatomy and Histology, Bosch Institute, Sydney Medical School, University of Sydney, NSW 2006, Australia; Biomolecular and Physical Sciences, Griffith University, QLD 4111, Australia.
authors.forename: Michael W
authors.initials: MW
authors.lastname: Weible
authors.affiliation: School of Chemistry, Key Centre for Polymers and Colloids, University of Sydney, NSW 2006, Australia.
authors.forename: Binh T T
authors.initials: BT
authors.lastname: Pham
authors.affiliation: School of Chemistry, Key Centre for Polymers and Colloids, University of Sydney, NSW 2006, Australia.
authors.forename: Brian S
authors.initials: BS
authors.lastname: Hawkett
authors.affiliation: Sydney Translational Imaging Laboratory, Sydney Medical School, University of Sydney, NSW 2006, Australia.
authors.forename: Stuart M
authors.initials: SM
authors.lastname: Grieve
authors.affiliation: Department of Anatomy and Histology, Bosch Institute, Sydney Medical School, University of Sydney, NSW 2006, Australia. Electronic address: tailoi@anatomy.usyd.edu.au.
authors.forename: Tailoi
authors.initials: T
authors.lastname: Chan-ling
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003911:Dextrans / Q000737:chemistry* / Q000494:pharmacology*; D006801:Humans; D008279:Magnetic Resonance Imaging; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D058953:Neural Stem Cells / Q000187:drug effects* / Q000378:metabolism; D011092:Polyethylene Glycols / Q000737:chemistry / Q000494:pharmacology; D012235:Rhodamines / Q000737:chemistry / Q000494:pharmacology
chemical_list: D003911:Dextrans; D058185:Magnetite Nanoparticles; D012235:Rhodamines; C097921:ferumoxtran-10; D011092:Polyethylene Glycols; C029773:rhodamine B
keywords: Biocompatibility; Iron oxide nanoparticles; MRI (magnetic resonance imaging); Neural cell; Regenerative medicine; Stem cells