title: Detection and molecular characterization of J subgroup avian leukosis virus in wild ducks in China.
abstract: To assess the status of avian leukosis virus subgroup J (ALV-J) in wild ducks in China, we examined samples from 528 wild ducks, representing 17 species, which were collected in China over the past 3 years. Virus isolation and PCR showed that 7 ALV-J strains were isolated from wild ducks. The env genes and the 3'UTRs from these isolates were cloned and sequenced. The env genes of all 7 wild duck isolates were significantly different from those in the prototype strain HPRS-103, American strains, broiler ALV-J isolates and Chinese local chicken isolates, but showed close homology with those found in some layer chicken ALV-J isolates and belonged to the same group. The 3'UTRs of 7 ALV-J wild ducks isolates showed close homology with the prototype strain HPRS-103 and no obvious deletion was found in the 3'UTR except for a 1 bp deletion in the E element that introduced a binding site for c-Ets-1. Our study demonstrated the presence of ALV-J in wild ducks and investigated the molecular characterization of ALV-J in wild ducks isolates.
authors.affiliation: College of Wildlife Resources, Northeast Forestry University, Harbin, China.
authors.forename: Xiangwei
authors.initials: X
authors.lastname: Zeng
authors.affiliation: College of Basic Medical Science, Heilongjiang University of Chinese Medicine, Harbin, China.
authors.forename: Lanlan
authors.initials: L
authors.lastname: Liu
authors.affiliation: College of Wildlife Resources, Northeast Forestry University, Harbin, China.
authors.forename: Ruijun
authors.initials: R
authors.lastname: Hao
authors.affiliation: College of Wildlife Resources, Northeast Forestry University, Harbin, China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Han
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000818:Animals; D000835:Animals, Wild / Q000821:virology*; D001354:Avian Leukosis Virus / Q000235:genetics* / Q000302:isolation & purification*; D001483:Base Sequence; D002681:China; D004372:Ducks / Q000821:virology*; D015752:Genes, env; D010802:Phylogeny
chemical_list: D020413:3' Untranslated Regions
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