title: The diagnostic importance of matrix metalloproteinase-7 and nestin in gastrointestinal stromal tumors.
abstract: BACKGROUND
  The importance of the matrix metalloproteinase-7 (MMP-7) and nestin immunomarkers, C-kit proto-oncogene (CD117), and the efficiency of the Ki-67 proliferation index for gastrointestinal stromal tumors were evaluated.
  
  
  METHODS
  This study was conducted by examining the microscope slides of 72 patients with gastrointestinal stromal tumors that were sent to the pathology laboratory between 2007 and 2012. Immunohistochemical staining for CD117, MMP-7, nestin, and marker of proliferation Ki-67 was performed. The correlations between the positive results for Ki-67, CD117, MMP-7, and nestin were evaluated relative to the tumor characteristics of size, localization, grade, cellular type, cellularity, cytology type, growth pattern, ulceration, necrosis, hemorrhage, invasion depth, and lymph node metastasis.
  
  
  RESULTS
  The tumor was localized in the stomach in 42 of the patients, the intestines in 19, the colon in 7, and the rectum in 4. Comparisons among the groups showed that MMP-7 was correlated with the tumor grade (p<0.001), cellularity (p<0.009), cytologic atypia (p<0.001), ulceration (p=0.002), necrosis (p<0.001), and tumor size (p=0.001). Nestin was correlated with the tumor grade (p=0.013), and tumor size (p=0.024). Correlations among CD117, MMP-7, nestin, and Ki-67 were examined. Nestin and Ki-67 were both significantly correlated with CD117 and MMP-7 [(r=0.279, p=0.018), (r=0.322, p=0.006), (r=0.386, p=0.001), (r=0.386, p=0.002)], respectively.
  
  
  CONCLUSIONS
  MMP-7 and nestin may be beneficial as markers, given their sensitivity to gastrointestinal stromal tumors.
authors.affiliation: Department of General Surgery, Erzincan University, Erzincan, Turkey.
authors.forename: Kemal
authors.initials: K
authors.lastname: Peker
authors.affiliation: Department of Pathology, Erzincan University, Erzincan, Turkey.
authors.forename: Ilyas
authors.initials: I
authors.lastname: Sayar
authors.affiliation: Department of Pathology, Namik Kemal University, Tekirdag, Turkey.
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Gelincik
authors.affiliation: Department of Pathology, Yüzüncü Yil University, Van, Turkey.
authors.forename: Gülay
authors.initials: G
authors.lastname: Bulut
authors.affiliation: Department of Pathology, Kayseri Training Research Hospital, Kayseri, Turkey.
authors.forename: Tuba Dilay
authors.initials: TD
authors.lastname: Kökenek Ünal
authors.affiliation: Department of Pathology, Medeniyet University, İstanbul, Turkey.
authors.forename: Serkan
authors.initials: S
authors.lastname: Şenol
authors.affiliation: Department of Pathology, Dişkapi Training Research Hospital, Ankara, Turkey.
authors.forename: Aysun
authors.initials: A
authors.lastname: Gökçe
authors.affiliation: Department of General Surgery, Erzincan University, Erzincan, Turkey.
authors.forename: Arda
authors.initials: A
authors.lastname: Isik
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002467:Cell Nucleus / Q000473:pathology; D046152:Gastrointestinal Stromal Tumors / Q000175:diagnosis* / Q000201:enzymology* / Q000473:pathology; D006801:Humans; D019394:Ki-67 Antigen / Q000378:metabolism; D020783:Matrix Metalloproteinase 7 / Q000378:metabolism*; D008875:Middle Aged; D064231:Nestin / Q000378:metabolism*; D000090063:Proto-Oncogene Mas; D019009:Proto-Oncogene Proteins c-kit / Q000378:metabolism; D013194:Staining and Labeling
chemical_list: D019394:Ki-67 Antigen; C000715470:MAS1 protein, human; C577484:NES protein, human; D064231:Nestin; D000090063:Proto-Oncogene Mas; D019009:Proto-Oncogene Proteins c-kit; C060932:MMP7 protein, human; D020783:Matrix Metalloproteinase 7
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