title: Targeting vertebrate intron-encoded box C/D 2'-O-methylation guide RNAs into the Cajal body.
abstract: Post-transcriptional pseudouridylation and 2'-O-methylation of splicesomal small nuclear ribonucleic acids (snRNAs) is mediated by box H/ACA and box C/D small Cajal body (CB)-specific ribonucleoproteins (scaRNPs), respectively. The WD-repeat protein 79 (WDR79) has been proposed to interact with both classes of modification scaRNPs and target them into the CB. The box H/ACA scaRNAs carry the common CAB box motif (consensus, ugAG) that is required for both WDR79 binding and CB-specific accumulation. Thus far, no cis-acting CB-localization element has been reported for vertebrate box C/D scaRNAs. In this study, systematic mutational analysis of the human U90 and another newly identified box C/D scaRNA, mgU2-47, demonstrated that the CB-specific accumulation of vertebrate intron-encoded box C/D scaRNAs relies on GU- or UG-dominated dinucleotide repeat sequences which are predicted to form the terminal stem-loop of the RNA apical hairpin. While the loop nucleotides are unimportant, the adjacent terminal helix that is composed mostly of consecutive G.U and U.G wobble base-pairs is essential for CB-specific localization of box C/D scaRNAs. Co-immunoprecipitation experiments confirmed that the newly identified CB localization element, called the G.U/U.G wobble stem, is crucial for in vivo association of box C/D scaRNPs with WDR79.
authors.affiliation: Laboratoire de Biologie Moléculaire Eucaryote du CNRS, UMR5099, IFR109 CNRS, Université Paul Sabatier, 118 route de Narbonne, 31062 Toulouse Cedex 9, France.
authors.forename: Aline
authors.initials: A
authors.lastname: Marnef
authors.affiliation: Laboratoire de Biologie Moléculaire Eucaryote du CNRS, UMR5099, IFR109 CNRS, Université Paul Sabatier, 118 route de Narbonne, 31062 Toulouse Cedex 9, France.
authors.forename: Patrica
authors.initials: P
authors.lastname: Richard
authors.affiliation: Laboratoire de Biologie Moléculaire Eucaryote du CNRS, UMR5099, IFR109 CNRS, Université Paul Sabatier, 118 route de Narbonne, 31062 Toulouse Cedex 9, France.
authors.forename: Natalia
authors.initials: N
authors.lastname: Pinzón
authors.affiliation: Laboratoire de Biologie Moléculaire Eucaryote du CNRS, UMR5099, IFR109 CNRS, Université Paul Sabatier, 118 route de Narbonne, 31062 Toulouse Cedex 9, France Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary tamas@ibcg.biotoul.fr.
authors.forename: Tamás
authors.initials: T
authors.lastname: Kiss
mesh_terms: D020541:Coiled Bodies / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D007438:Introns; D018832:Molecular Chaperones; D009690:Nucleic Acid Conformation; D011506:Proteins / Q000378:metabolism; D012342:RNA, Small Nuclear / Q000737:chemistry / Q000378:metabolism; D020537:RNA, Small Nucleolar / Q000737:chemistry* / Q000378:metabolism*; D012091:Repetitive Sequences, Nucleic Acid; D019098:Telomerase; D058727:RNA, Small Untranslated
chemical_list: D018832:Molecular Chaperones; D011506:Proteins; D012342:RNA, Small Nuclear; D020537:RNA, Small Nucleolar; D019098:Telomerase; C535133:WRAP53 protein, human; D058727:RNA, Small Untranslated
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