title: The X-ray crystal structure of Shewanella oneidensis OmcA reveals new insight at the microbe-mineral interface.
abstract: The X-ray crystal structure of Shewanella oneidensis OmcA, an extracellular decaheme cytochrome involved in mineral reduction, was solved to a resolution of 2.7 Å. The four OmcA molecules in the asymmetric unit are arranged so the minimum distance between heme 5 on adjacent OmcA monomers is 9 Å, indicative of a transient OmcA dimer capable of intermolecular electron transfer. A previously identified hematite binding motif was identified near heme 10, forming a hydroxylated surface that would bring a heme 10 electron egress site to ∼10 Å of a mineral surface.
authors.affiliation: Centre for Molecular and Structural Biochemistry, School of Biological Sciences and School of Chemistry, University of East Anglia, Norwich NR4 7TJ, United Kingdom.
authors.forename: Marcus J
authors.initials: MJ
authors.lastname: Edwards
authors.affiliation: Centre for Molecular and Structural Biochemistry, School of Biological Sciences and School of Chemistry, University of East Anglia, Norwich NR4 7TJ, United Kingdom.
authors.forename: Nanakow A
authors.initials: NA
authors.lastname: Baiden
authors.affiliation: Environmental Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA.
authors.forename: Alexander
authors.initials: A
authors.lastname: Johs
authors.affiliation: Environmental Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA.
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Tomanicek
authors.affiliation: Environmental Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA.
authors.forename: Liyuan
authors.initials: L
authors.lastname: Liang
authors.affiliation: Pacific Northwest National Laboratory, Richland, WA 99352, USA.
authors.forename: Liang
authors.initials: L
authors.lastname: Shi
authors.affiliation: Pacific Northwest National Laboratory, Richland, WA 99352, USA.
authors.forename: Jim K
authors.initials: JK
authors.lastname: Fredrickson
authors.affiliation: Pacific Northwest National Laboratory, Richland, WA 99352, USA.
authors.forename: John M
authors.initials: JM
authors.lastname: Zachara
authors.affiliation: Centre for Molecular and Structural Biochemistry, School of Biological Sciences and School of Chemistry, University of East Anglia, Norwich NR4 7TJ, United Kingdom.
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Gates
authors.affiliation: Centre for Molecular and Structural Biochemistry, School of Biological Sciences and School of Chemistry, University of East Anglia, Norwich NR4 7TJ, United Kingdom.
authors.forename: Julea N
authors.initials: JN
authors.lastname: Butt
authors.affiliation: Centre for Molecular and Structural Biochemistry, School of Biological Sciences and School of Chemistry, University of East Anglia, Norwich NR4 7TJ, United Kingdom. Electronic address: d.richardson@uea.ac.uk.
authors.forename: David J
authors.initials: DJ
authors.lastname: Richardson
authors.affiliation: Centre for Molecular and Structural Biochemistry, School of Biological Sciences and School of Chemistry, University of East Anglia, Norwich NR4 7TJ, United Kingdom. Electronic address: tom.clarke@uea.ac.uk.
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Clarke
mesh_terms: D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D001665:Binding Sites / Q000235:genetics; D018360:Crystallography, X-Ray; D006418:Heme / Q000737:chemistry / Q000378:metabolism; D006900:Hydroxylation; D008958:Models, Molecular; D008969:Molecular Sequence Data; D055503:Protein Multimerization; D017434:Protein Structure, Tertiary; D053838:Scattering, Small Angle; D017386:Sequence Homology, Amino Acid; D020592:Shewanella / Q000235:genetics / Q000378:metabolism*; D014961:X-Ray Diffraction
chemical_list: D001425:Bacterial Outer Membrane Proteins; D006418:Heme
keywords: Electron transfer; Metalloprotein; Mineral respiration; Multiheme cytochrome; Outer membrane; Shewanella; c-Type heme