title: Altered lysophosphatidic acid (LPA) receptor expression during hepatic regeneration in a mouse model of partial hepatectomy.
abstract: BACKGROUND
  Hepatic regeneration requires coordinated signal transduction for efficient restoration of functional liver mass. This study sought to determine changes in lysophosphatidic acid (LPA) and LPA receptor (LPAR) 1-6 expression in regenerating liver following two-thirds partial hepatectomy (PHx).
  
  
  METHODS
  Liver tissue and blood were collected from male C57BL/6 mice following PHx. Circulating LPA was measured by enzyme-linked immunosorbent assay (ELISA) and hepatic LPAR mRNA and protein expression were determined.
  
  
  RESULTS
  Circulating LPA increased 72 h after PHx and remained significantly elevated for up to 7 days post-PHx. Analysis of LPAR expression after PHx demonstrated significant increases in LPAR1, LPAR3 and LPAR6 mRNA and protein in a time-dependent manner for up to 7 days post-PHx. Conversely, LPAR2, LPAR4 and LPAR5 mRNA were barely detected in normal liver and did not significantly change after PHx. Changes in LPAR1 expression were confined to non-parenchymal cells following PHx.
  
  
  CONCLUSIONS
  Liver regeneration following PHx is associated with significant changes in circulating LPA and hepatic LPAR1, LPAR3 and LPAR6 expression in a time- and cell-dependent manner. Furthermore, changes in LPA-LPAR post-PHx occur after the first round of hepatocyte division is complete.
authors.affiliation: Department of Surgery, Carolinas Medical Center, Charlotte, NC, USA.
authors.forename: Kerri A
authors.initials: KA
authors.lastname: Simo
authors.forename: David J
authors.initials: DJ
authors.lastname: Niemeyer
authors.forename: Erin M
authors.initials: EM
authors.lastname: Hanna
authors.forename: Jacob H
authors.initials: JH
authors.lastname: Swet
authors.forename: Kyle J
authors.initials: KJ
authors.lastname: Thompson
authors.forename: David
authors.initials: D
authors.lastname: Sindram
authors.forename: David A
authors.initials: DA
authors.lastname: Iannitti
authors.forename: Ashley L
authors.initials: AL
authors.lastname: Eheim
authors.forename: Eugene
authors.initials: E
authors.lastname: Sokolov
authors.forename: Valentina
authors.initials: V
authors.lastname: Zuckerman
authors.forename: Iain H
authors.initials: IH
authors.lastname: McKillop
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D005786:Gene Expression Regulation; D006498:Hepatectomy / Q000379:methods*; D008099:Liver / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology / Q000601:surgery*; D008115:Liver Regeneration; D008246:Lysophospholipids / Q000378:metabolism; D008297:Male; D008810:Mice, Inbred C57BL; D023421:Models, Animal; D012333:RNA, Messenger / Q000378:metabolism; D049368:Receptors, Lysophosphatidic Acid / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D013997:Time Factors
chemical_list: D008246:Lysophospholipids; D012333:RNA, Messenger; D049368:Receptors, Lysophosphatidic Acid; C032881:lysophosphatidic acid
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