title: Effect of bovine adenovirus 3 on mitochondria.
abstract: Viruses alter the structure and the function of mitochondria for survival. Electron microscopy analysis of the cells infected with bovine adenovirus 3 revealed extensive damage to the inner mitochondrial membrane characterized by dissolution of the cristae and amorphous appearance of mitochondrial matrix with little or no damage to the outer mitochondrial membrane. There were fewer cristae with altered morphology. Potential patches of protein synthesis machinary around mitochondria could be observed at 12 hours post infection (hpi). At 24 hpi, the multi vascular bodies were evident throughout the infected cell. ATP production, mitochondrial Ca2+ and mitochondrial membrane potential (MMP) peaked at 18 hpi but decreased significantly at 24 hpi. This decrease coincided with the increased production of superoxide (SO) and reactive oxygen species (ROS), at 24 hpi indicating acute oxidative stress in the cells and suggesting a complete failure of the cellular homeostatic machinary. The results reveal an intericate relationship between Ca2+ homeostasis, the ATP generation ability of cells, SO and ROS production, and regulation of MMP following infection by bovine adenovirus 3.
authors.forename: Sanjeev K
authors.initials: SK
authors.lastname: Anand
authors.forename: Jaswant
authors.initials: J
authors.lastname: Singh
authors.forename: Amit
authors.initials: A
authors.lastname: Gaba
authors.affiliation: Vaccine and Infectious Disease Organization -International Vaccine Center (VIDO- InterVac), University of Saskatchewan, Saskatoon, SK S7N 5E3, Canada. Suresh.tik@usask.ca.
authors.forename: Suresh K
authors.initials: SK
authors.lastname: Tikoo
mesh_terms: D000257:Adenoviridae Infections / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D000818:Animals; D015153:Blotting, Western / Q000662:veterinary; D002417:Cattle; D002418:Cattle Diseases / Q000473:pathology / Q000821:virology*; D002460:Cell Line; D017921:Mastadenovirus / Q000502:physiology*; D053078:Membrane Potential, Mitochondrial; D046529:Microscopy, Electron, Transmission / Q000662:veterinary; D008928:Mitochondria / Q000821:virology*; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism; D014779:Virus Replication
chemical_list: D017382:Reactive Oxygen Species; D013481:Superoxides
references.citation: Can Vet J. 1981 Mar;22(3):68-71
references.pmid: 6269717
references.citation: Vojnosanit Pregl. 2010 Dec;67(12):1015-20
references.pmid: 21425559
references.citation: Apoptosis. 2000 Jun;5(3):211-5
references.pmid: 11225841
references.citation: J Exp Med. 1954 Sep 1;100(3):301-10
references.pmid: 13192254
references.citation: J Cell Biol. 1964 Dec;23:499-509
references.pmid: 14245433
references.citation: Virol J. 2006 Aug 31;3:62
references.pmid: 16942625
references.citation: Biochem Biophys Res Commun. 2014 Jan 10;443(2):464-9
references.pmid: 24321549
references.citation: Oncogene. 2001 Nov 26;20(54):7836-46
references.pmid: 11753666
references.citation: Physiol Rev. 1999 Oct;79(4):1127-55
references.pmid: 10508231
references.citation: Cell. 2006 Jun 30;125(7):1241-52
references.pmid: 16814712
references.citation: Biochim Biophys Acta. 2006 Sep-Oct;1757(9-10):1429-37
references.pmid: 16814246
references.citation: J Cell Biol. 1966 Aug;30(2):269-97
references.pmid: 5968972
references.citation: Toxicol Sci. 2002 Feb;65(2):166-76
references.pmid: 11812920
references.citation: FEBS Lett. 2003 Jul 3;546(1):113-20
references.pmid: 12829246
references.citation: Biochem Biophys Res Commun. 2011 Jan 7;404(1):364-9
references.pmid: 21134354
references.citation: FEBS Lett. 2011 Jan 3;585(1):99-103
references.pmid: 21081127
references.citation: Can J Biochem Cell Biol. 1985 Nov;63(11):1194-7
references.pmid: 4084857
references.citation: Symp Soc Exp Biol. 2000;52:241-51
references.pmid: 12090012
references.citation: Biochim Biophys Acta. 2009 Nov;1787(11):1334-41
references.pmid: 19481532
references.citation: J Gen Virol. 1998 May;79 ( Pt 5):1033-45
references.pmid: 9603318
references.citation: J Virol. 2011 Oct;85(20):10806-13
references.pmid: 21835790
references.citation: Mitochondrion. 2004 Jul;4(2-3):223-33
references.pmid: 16120388
references.citation: Oncogene. 2005 Oct 13;24(45):6796-808
references.pmid: 16007153
references.citation: J Physiol. 1998 Mar 1;507 ( Pt 2):405-14
references.pmid: 9518701
references.citation: Biochem Biophys Res Commun. 2007 Jun 22;358(1):203-8
references.pmid: 17475217
references.citation: J Biomed Sci. 2009 Sep 02;16:80
references.pmid: 19725950
references.citation: Virology. 1996 Jun 1;220(1):152-62
references.pmid: 8659107
references.citation: J Virol. 1998 Feb;72(2):1394-402
references.pmid: 9445040
references.citation: Curr Top Dev Biol. 2007;77:113-55
references.pmid: 17222702
references.citation: Nature. 1998 Oct 15;395(6703):645-8
references.pmid: 9790183
references.citation: HIV Med. 2011 Feb;12(2):78-86
references.pmid: 20500231
references.citation: Virology. 1999 Jan 20;253(2):299-308
references.pmid: 9918888
references.citation: EMBO Rep. 2003 Oct;4(10):948-52
references.pmid: 14528265
references.citation: J Virol. 2006 Jul;80(14):7199-207
references.pmid: 16809325
references.citation: Ion Channels. 1996;4:169-202
references.pmid: 8744209
references.citation: Adv Virol. 2013;2013:738794
references.pmid: 24260034
references.citation: Biochem J. 2000 Jun 1;348 Pt 2:425-32
references.pmid: 10816438
references.citation: J Virol. 2003 Jan;77(1):631-41
references.pmid: 12477866
references.citation: EMBO J. 2001 Jun 1;20(11):2690-701
references.pmid: 11387204
references.citation: Virology. 2004 May 20;323(1):59-69
references.pmid: 15165819
references.citation: J Exp Med. 2000 May 1;191(9):1487-98
references.pmid: 10790424
references.citation: Cell Biol Toxicol. 1999;15(6):367-73
references.pmid: 10811531
references.citation: Virology. 2001 Nov 25;290(2):329-41
references.pmid: 11883197
references.citation: Methods. 2002 Apr;26(4):335-40
references.pmid: 12054924
references.citation: J Exp Med. 1961 Jul 1;114:1-12
references.pmid: 13741584
references.citation: Curr Opin Cell Biol. 2003 Dec;15(6):706-16
references.pmid: 14644195
references.citation: J Cell Biochem. 2011 Jan;112(1):233-43
references.pmid: 21069731
references.citation: J Gen Virol. 2014 Feb;95(Pt 2):442-452
references.pmid: 24123521
references.citation: Cell Calcium. 2000 Feb;27(2):97-106
references.pmid: 10756976
references.citation: Cell Res. 2006 Feb;16(2):141-7
references.pmid: 16474426
references.citation: J Exp Med. 1961 Jan 1;113:219-34
references.pmid: 13772566