title: Potential of spectroscopic techniques and chemometric analysis for rapid measurement of docosahexaenoic acid and eicosapentaenoic acid in algal oil.
abstract: Developing rapid methods for measuring long-chain ω-3 (n-3) poly-unsaturated fatty acid (LCPUFA) contents has been a crucial request from the algal oil industry. In this study, four spectroscopy techniques, namely visible and short-wave near infra-red (Vis-SNIR), long-wave near infra-red (LNIR), mid-infra-red (MIR) and nuclear magnetic resonance (NMR) spectroscopy, were exploited for determining the docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) contents in algal oil. The best prediction for both DHA and EPA were achieved by NMR spectroscopy, in which the determination coefficients of cross-validation (rCV(2)) values were 0.963 and 0.967 for two LCPUFAs. The performances of Vis-SNIR and LNIR spectroscopy were also accepted. The variable selection was proved as an efficient and necessary step for the spectral analysis in this study. The results were promising and implied that spectroscopy techniques have a great potential for assessment of DHA and EPA in algal oil.
authors.affiliation: College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China. Electronic address: china.di.wu@gmail.com.
authors.forename: Di
authors.initials: D
authors.lastname: Wu
authors.affiliation: College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China. Electronic address: yhe@zju.edu.cn.
authors.forename: Yong
authors.initials: Y
authors.lastname: He
mesh_terms: D004281:Docosahexaenoic Acids / Q000737:chemistry*; D015118:Eicosapentaenoic Acid / Q000737:chemistry*; D005231:Fatty Acids, Unsaturated; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D010938:Plant Oils / Q000737:chemistry*; D013057:Spectrum Analysis
chemical_list: D005231:Fatty Acids, Unsaturated; D010938:Plant Oils; D004281:Docosahexaenoic Acids; D015118:Eicosapentaenoic Acid
keywords: Algal oil; Docosahexaenoic acid (DHA); Eicosapentaenoic acid (EPA); Mid-infra-red spectroscopy; Nuclear magnetic resonance; Poly-unsaturated fatty acids; Visible and near infra-red spectroscopy