title: Heterologous production of the lipopeptide biosurfactant serrawettin W1 in Escherichia coli.
abstract: The non-ionic biosurfactant serrawettin W1 is a lipopeptide produced by red-pigmented strains of Serratia marcescens which shows antimicrobial, antitumor and plant protecting properties. Here, we report a simple method for heterologous production of this biosurfactant. S. marcescens strain DSM12481 was identified as a novel serrawettin W1 producer and the respective nonribosomal peptide synthetase gene swrW was cloned and expressed in Escherichia coli BL21 Gold. Chemical analysis of heterologous serrawettin W1 revealed that E. coli mainly produced serrawettin with C10 fatty acids as does S. marcescens. Additionally, serrawettin species with longer fatty acids (C13, C14) were detected in S. marcescens which were absent in E. coli. The expression system described here paves the way for the large scale production of this biotechnologically important biosurfactant.
authors.affiliation: Institut für Molekulare Enzymtechnologie, Heinrich-Heine-Universität Düsseldorf and Institut für Bio- und Geowissenschaften IBG-1, Forschungszentrum Jülich, D-52426 Jülich, Germany.
authors.forename: Stephan
authors.initials: S
authors.lastname: Thies
authors.affiliation: Central Division of Analytical Chemistry ZEA-3: Analytik/Biospec, Forschungszentrum Jülich, D-52426 Jülich, Germany.
authors.forename: Beatrix
authors.initials: B
authors.lastname: Santiago-Schübel
authors.affiliation: Institut für Molekulare Enzymtechnologie, Heinrich-Heine-Universität Düsseldorf and Institut für Bio- und Geowissenschaften IBG-1, Forschungszentrum Jülich, D-52426 Jülich, Germany.
authors.forename: Filip
authors.initials: F
authors.lastname: Kovačić
authors.affiliation: Institut für Pharmazeutische Biotechnologie, Universität Ulm, D-89081 Ulm, Germany.
authors.forename: Frank
authors.initials: F
authors.lastname: Rosenau
authors.affiliation: University of Hohenheim, Institute of Food Science and Biotechnology, Bioprocess Engineering, Stuttgart, Germany.
authors.forename: Rudolf
authors.initials: R
authors.lastname: Hausmann
authors.affiliation: Institut für Molekulare Enzymtechnologie, Heinrich-Heine-Universität Düsseldorf and Institut für Bio- und Geowissenschaften IBG-1, Forschungszentrum Jülich, D-52426 Jülich, Germany. Electronic address: karl-erich.jaeger@fz-juelich.de.
authors.forename: Karl-Erich
authors.initials: KE
authors.lastname: Jaeger
mesh_terms: D047630:Depsipeptides / Q000096:biosynthesis* / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005227:Fatty Acids / Q000737:chemistry; D005285:Fermentation; D055666:Lipopeptides / Q000096:biosynthesis* / Q000235:genetics; D012706:Serratia marcescens / Q000378:metabolism; D013501:Surface-Active Agents / Q000378:metabolism
chemical_list: D047630:Depsipeptides; D005227:Fatty Acids; D055666:Lipopeptides; D013501:Surface-Active Agents; C009246:serratamolide
keywords: Biosurfactant; Heterologous expression; Serratia marcescens; Serrawettin W1