title: Isolation and identification of α-glucosidase and protein glycation inhibitors from Stereospermum colais.
abstract: Stereospermum colais (family Bignoniaceae) is a well-known pharmacologically potent medicinal plant reported in traditional systems of medicine. Phytochemical investigation of the roots of S. colais resulted in the isolation of seven compounds, and the metabolites were screened for its α-glucosidase enzyme inhibition and anti-glycation property. The compounds identified were β-sitosterol (1), 2-(4'-hydroxyphenyl) ethyl undecanoate (2), 2-(4'-hydroxyphenyl)ethyl pentadecanoate (3), 5α-ergosta-7,22-dien-3β-ol (4), ursolic acid (5), lapachol (6), and pinoresinol (7). Ursolic acid, lapachol, and pinoresinol possessed IC50 values of 119.01, 130.29, and 125.62 nM, respectively, compared to standard ascorbic acid with an IC50 value of 201.01 nM. The other compounds failed to show the activity. Results of the current study showcased the possible exploration of this medicinal plant for the treatment of type 2 diabetes in line with the development of phytopharmaceutical industry.
authors.affiliation: Agroprocessing and Natural Products Division, National Institute for Interdisciplinary Science and Technology (NIIST), CSIR, Trivandrum, Kerala, 695019, India.
authors.forename: M Priya
authors.initials: MP
authors.lastname: Rani
authors.forename: K G
authors.initials: KG
authors.lastname: Raghu
authors.forename: Mangalam S
authors.initials: MS
authors.lastname: Nair
authors.forename: K P
authors.initials: KP
authors.lastname: Padmakumari
mesh_terms: D029402:Bignoniaceae / Q000737:chemistry*; D003924:Diabetes Mellitus, Type 2 / Q000188:drug therapy; D004791:Enzyme Inhibitors / Q000737:chemistry* / Q000302:isolation & purification* / Q000627:therapeutic use; D006031:Glycosylation; D006801:Humans; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification; D018517:Plant Roots / Q000737:chemistry*; D000520:alpha-Glucosidases / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D004791:Enzyme Inhibitors; D010940:Plant Proteins; D000520:alpha-Glucosidases