title: [Analytical validation of a chromatographic method dedicated to search and identify natural and semi-synthetic opiates].
abstract: The identification of a product absorbed by an opiate consumer is sometimes problematic since there is no specific biomarker for all molecules. We developed an ultra-high pressure liquid chromatography coupled to tandem mass spectrometry technique which allows the identification and the quantification of 25 opiates in plasma. The sample preparation consists in a solid-phase extraction on Oasis MCX cartridges (Waters). The method has been validated according to FDA criteria completely for 21 substances and with some reservations for the remaining 4 analytes. This method has been applied to 80 patients treated at the University Hospital of Liege for whom the screening of opiates was positive. The identification of the product consumed was effective in 86% of cases.
authors.affiliation: Service de toxicologie clinique, médicolégale, de l'environnement et en entreprise, CHU, Liège, Belgique.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Dubois
authors.affiliation: Service de chimie pharmaceutique, Département de pharmacie, Université de Liège, Belgique.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Counerotte
authors.affiliation: Service de chimie pharmaceutique, Département de pharmacie, Université de Liège, Belgique.
authors.forename: Eric
authors.initials: E
authors.lastname: Goffin
authors.affiliation: Service de chimie pharmaceutique, Département de pharmacie, Université de Liège, Belgique, CIRM, Université de Liège, Belgique.
authors.forename: Bernard
authors.initials: B
authors.lastname: Pirotte
authors.affiliation: Service de toxicologie clinique, médicolégale, de l'environnement et en entreprise, CHU, Liège, Belgique, CIRM, Université de Liège, Belgique.
authors.forename: Corinne
authors.initials: C
authors.lastname: Charlier
mesh_terms: D001774:Blood Chemical Analysis / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D053610:Opiate Alkaloids / Q000032:analysis / Q000097:blood*; D018847:Opioid Peptides / Q000032:analysis / Q000097:blood*; D052616:Solid Phase Extraction; D015813:Substance Abuse Detection / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D053610:Opiate Alkaloids; D018847:Opioid Peptides
keywords: LC-MS; analytical validation; identification; opiates; plasma