title: Model-guided identification of gene deletion targets for metabolic engineering in Saccharomyces cerevisiae.
abstract: Identification of metabolic engineering strategies for rerouting intracellular fluxes towards a desired product is often a challenging task owing to the topological and regulatory complexity of metabolic networks. Genome-scale metabolic models help tackling this complexity through systematic consideration of mass balance and reaction directionality constraints over the entire network. Here, we describe how genome-scale metabolic models can be used for identifying gene deletion targets leading to increased production of the desired product. Vanillin production in Saccharomyces cerevisiae is used as a case study throughout this chapter.
authors.affiliation: Genome Biology Unit, European Molecular Biology Laboratory, Meyerhofstraße 1, Heidelberg, 69117, Germany.
authors.forename: Ana Rita
authors.initials: AR
authors.lastname: Brochado
authors.forename: Kiran Raosaheb
authors.initials: KR
authors.lastname: Patil
mesh_terms: D017353:Gene Deletion; D060847:Metabolic Engineering / Q000379:methods*; D008954:Models, Biological; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*