title: Operation performance and microbial community dynamics of phosphorus removal sludge with different electron acceptors.
abstract: Operation performances of phosphorus removal sludge with different electron acceptors in three parallel SBRs were firstly compared in the present study, and the effect of post-aeration on denitrifying phosphorus removal was also studied. Moreover, community dynamics of different phosphorus removal sludge was systematically investigated with high-throughput sequencing for the first time. TP removal rates for nitrate-, nitrite-, and oxygen-based phosphorus removal sludge were 84.8, 78.5, and 87.4 %, with an average effluent TP concentration of 0.758, 0.931, and 0.632 mg/l. The average specific phosphorus release and uptake rates were 20.3, 10.8, and 21.5, and 9.43, 8.68, and 10.8 mgP/(gVSS h), respectively. Moreover, electron utilization efficiency of denitrifying phosphorus removal sludge with nitrate as electron acceptor was higher than nitrite, with P/e(-) were 2.21 and 1.51 mol-P/mol-e(-), respectively. With the assistance of post-aeration for nitrate-based denitrifying phosphorus removal sludge, settling ability could be improved, with SVI decreased from 120 to 80 and 72 ml/g when post-aeration time was 0, 10, and 30 min, respectively. Moreover, further phosphorus removal could be achieved during post-aeration with increased aeration time. However, the anoxic phosphorus uptake was deteriorated, which was likely a result of shifted microbial community structure. Post-aeration of approximately 10 min was proposed for denitrifying phosphorus removal. Nitrate- and nitrite-based denitrifying phosphorus removal sludge exhibited similar community structure. More phosphorus accumulating organisms were enriched under anaerobic-aerobic conditions, while anaerobic-anoxic conditions were favorable for suppressing glycogen-accumulating organisms. Significant differences in pathogenic bacterial community profiles revealed in the current study indicated the potential public health hazards of non-aeration activated sludge system.
authors.affiliation: Environmental Science and Engineering Center, Harbin Institute of Technology Shenzhen Graduate School, Shenzhen, 518055, Guangdong, People's Republic of China.
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Lv
authors.forename: Mingfei
authors.initials: M
authors.lastname: Shao
authors.forename: Chaolin
authors.initials: C
authors.lastname: Li
authors.forename: Ji
authors.initials: J
authors.lastname: Li
authors.forename: Dongyang
authors.initials: D
authors.lastname: Liu
authors.forename: Xinlei
authors.initials: X
authors.lastname: Gao
authors.forename: Xue
authors.initials: X
authors.lastname: Xia
mesh_terms: D019149:Bioreactors; D058440:Denitrification; D004583:Electrons; D009566:Nitrates / Q000378:metabolism; D009573:Nitrites / Q000378:metabolism; D016877:Oxidants / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D010758:Phosphorus / Q000302:isolation & purification* / Q000378:metabolism; D012722:Sewage / Q000382:microbiology*
chemical_list: D009566:Nitrates; D009573:Nitrites; D016877:Oxidants; D012722:Sewage; D010758:Phosphorus; D010100:Oxygen
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