title: Differential expression of cysteine dioxygenase 1 in complex karyotype liposarcomas.
abstract: Altered cysteine dioxygenase 1 (CDO1) gene expression has been observed in several cancers but has not yet been investigated in liposarcomas. The aim of this study was to evaluate CDO1 expression in a cohort of liposarcomas and to determine its association with clinicopathological features. Existing microarray data indicated variable CDO1 expression in liposarcoma subtypes. CDO1 mRNA from a larger cohort of liposarcomas was quantified by real time-PCR, and CDO1 protein expression was determined by immunohistochemistry (IHC) in more than 300 tumor specimens. Well-differentiated liposarcomas (WDLSs) had significantly higher CDO1 gene expression and protein levels than dedifferentiated liposarcomas (DDLSs) (P < 0.001). Location of the tumor was not predictive of the expression level of CDO1 mRNA in any histological subtype of liposarcoma. Recurrent tumors did not show any difference in CDO1 expression when compared to primary tumors. CDO1 expression was upregulated as human mesenchymal stem cells (hMSCs) undergo differentiation into mature adipocytes. Our results suggest that CDO1 is a marker of liposarcoma progression and adipogenic differentiation.
authors.affiliation: Department of Laboratory Oncology Research, Curtis and Elizabeth Anderson Cancer Institute, Memorial University Medical Center, Savannah, GA, USA.
authors.forename: Mohammed
authors.initials: M
authors.lastname: Shaker
authors.affiliation: Department of Laboratory Oncology Research, Curtis and Elizabeth Anderson Cancer Institute, Memorial University Medical Center, Savannah, GA, USA.
authors.forename: Kara M
authors.initials: KM
authors.lastname: Pascarelli
authors.affiliation: Department of Laboratory Oncology Research, Curtis and Elizabeth Anderson Cancer Institute, Memorial University Medical Center, Savannah, GA, USA.
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Plantinga
authors.affiliation: Department of Biomedical Sciences, Mercer University School of Medicine-Savannah Campus, Savannah, GA, USA.
authors.forename: Miles A
authors.initials: MA
authors.lastname: Love
authors.affiliation: Department of Cancer Biology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.
authors.forename: Alexander J
authors.initials: AJ
authors.lastname: Lazar
authors.affiliation: Department of Cancer Biology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.
authors.forename: Davis R
authors.initials: DR
authors.lastname: Ingram
authors.affiliation: Department of Medical Oncology, Population Science Division and Human Genetics Program, Fox Chase Cancer Center, Philadelphia, PA, USA.
authors.forename: Margaret
authors.initials: M
authors.lastname: von Mehren
authors.affiliation: Department of Cancer Biology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.
authors.forename: Dina
authors.initials: D
authors.lastname: Lev
authors.affiliation: Institute of Cancer and Genetics, School of Medicine, Cardiff University, Cardiff, UK.
authors.forename: David
authors.initials: D
authors.lastname: Kipling
authors.affiliation: Department of Laboratory Oncology Research, Curtis and Elizabeth Anderson Cancer Institute, Memorial University Medical Center, Savannah, GA, USA. ; Department of Biomedical Sciences, Mercer University School of Medicine-Savannah Campus, Savannah, GA, USA.
authors.forename: Dominique
authors.initials: D
authors.lastname: Broccoli
keywords: adipocyte differentiation; cysteine dioxygenase 1; liposarcoma
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