title: Visual detection of norovirus genogroup II by reverse transcription loop-mediated isothermal amplification with hydroxynaphthol blue dye.
abstract: A simple, rapid, specific, and sensitive colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay with hydroxynaphthol blue dye (HNB) was established, targeting RNA-dependent RNA polymerase and capsid protein gene for the detection of the dominant norovirus genogroup in China-NoV GII. The assay was carried out at 65 °C for 60 min with no cross-reactivity with other common gastroenteritis viruses. The sensitivity of this assay was 10(3) copies per reaction which is equivalent to the conventional RT-PCR test. The clinical test showed 94.83% coincidence rate for NoV genogroup II detection compared with the results, confirmed by the Department of Viral Diarrhea of Chinese Center for Disease Control and Prevention via conventional RT-PCR. The HNB dye-based RT-LAMP could be a novel rapid screening method for prevalent norovirus genogroup II in China, especially in those resource-limited hospitals and rural local clinics.
authors.affiliation: Key Laboratory for Medical Virology, Ministry of Health, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 155 Changbai Road, Beijing, 102206, China.
authors.forename: Jianming
authors.initials: J
authors.lastname: Luo
authors.forename: Ziqian
authors.initials: Z
authors.lastname: Xu
authors.forename: Kai
authors.initials: K
authors.lastname: Nie
authors.forename: Xiong
authors.initials: X
authors.lastname: Ding
authors.forename: Li
authors.initials: L
authors.lastname: Guan
authors.forename: Ji
authors.initials: J
authors.lastname: Wang
authors.forename: Yuying
authors.initials: Y
authors.lastname: Xian
authors.forename: Xiyang
authors.initials: X
authors.lastname: Wu
authors.forename: Xuejun
authors.initials: X
authors.lastname: Ma
mesh_terms: D017250:Caliciviridae Infections / Q000821:virology*; D002681:China; D004396:Coloring Agents / Q000737:chemistry / Q000378:metabolism*; D005838:Genotype; D006801:Humans; D009282:Naphthalenesulfonates / Q000737:chemistry / Q000378:metabolism*; D029322:Norovirus / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D021141:Nucleic Acid Amplification Techniques / Q000295:instrumentation / Q000379:methods*; D048348:Reverse Transcription; D014764:Viral Proteins / Q000235:genetics
chemical_list: D004396:Coloring Agents; D009282:Naphthalenesulfonates; D014764:Viral Proteins; C540250:trisodium 3-hydroxy-4-((2Z)-2-(2-oxo-4-sulfonatonaphthalen-1-ylidene)hydrazinyl)naphthalene-2,7-disulfonate
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