title: Transient inactivation of the ventral hippocampus in neonatal rats impairs the mesolimbic regulation of prefrontal glutamate release in adulthood.
abstract: Cognitive deficits in schizophrenia (SZ) reflect maturational disruptions within a neural system that includes the ventral hippocampus (VH), nucleus accumbens (NAc), basal forebrain, and prefrontal cortex (PFC). A better understanding of these changes may reveal drug targets for more efficacious cognition enhancers. We have utilized an animal model in which the above distributed system is altered, during a sensitive period of development, by transiently inactivating the VH and its efferent projections. We determined the ability of NAc shell activation to evoke prefrontal glutamate release in adult male Wistar rats that had received saline (Sal) or tetrodotoxin (TTX) as neonates (PD7) or as adolescents (PD32). The nucleus accumbens shell (NAcSh) was activated by NMDA infusions (0.05-0.30 μg/0.5 μL). Basal and evoked glutamate levels were measured amperometrically using a glutamate-sensitive microelectrode. There were no differences in basal glutamate levels among the groups tested (overall 1.41 ± 0.26 uM). However, the dose-related stimulation of prefrontal glutamate levels seen in control rats treated with saline on PD7 (4.31 ± 0.22 μM after 0.15 μg) was markedly attenuated in rats treated with TTX on PD7 (0.45 ± 0.12 μM after 0.15 μg). This effect was age-dependent as infusions of TTX on PD32 did not alter the NMDA-induced increases in glutamate release (4.10 ± 0.37 μM after 0.15 μg). Collectively, these findings reveal that transient inactivation of VH transmission, during a sensitive period of development, leads to a functional mesolimbic-cortical disconnection that produces neurochemical and ultimately cognitive impairments resembling those seen in SZ.
authors.affiliation: Dept. of Psychology, The Ohio State University, Columbus, OH, USA.
authors.forename: D M
authors.initials: DM
authors.lastname: Bortz
authors.affiliation: Neurobiology Research Unit, University Hospital Rigshospitalet, Copenhagen, Denmark.
authors.forename: C V
authors.initials: CV
authors.lastname: Jørgensen
authors.affiliation: Neurobiology Research Unit, University Hospital Rigshospitalet, Copenhagen, Denmark.
authors.forename: J D
authors.initials: JD
authors.lastname: Mikkelsen
authors.affiliation: Dept. of Psychology, The Ohio State University, Columbus, OH, USA; Dept. of Neuroscience, The Ohio State University, Columbus, OH, USA. Electronic address: bruno.1@osu.edu.
authors.forename: J P
authors.initials: JP
authors.lastname: Bruno
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002408:Catheters, Indwelling; D004195:Disease Models, Animal; D004567:Electrodes, Implanted; D018698:Glutamic Acid / Q000378:metabolism*; D006624:Hippocampus / Q000187:drug effects / Q000254:growth & development* / Q000503:physiopathology*; D008297:Male; D008839:Microelectrodes; D016202:N-Methylaspartate / Q000378:metabolism; D009714:Nucleus Accumbens / Q000187:drug effects / Q000254:growth & development / Q000503:physiopathology; D017397:Prefrontal Cortex / Q000187:drug effects / Q000254:growth & development* / Q000503:physiopathology*; D017208:Rats, Wistar; D012559:Schizophrenia; D026941:Sodium Channel Blockers / Q000494:pharmacology; D013779:Tetrodotoxin / Q000494:pharmacology
chemical_list: D026941:Sodium Channel Blockers; D018698:Glutamic Acid; D013779:Tetrodotoxin; D016202:N-Methylaspartate
keywords: Animal models; Biosensor; Nucleus accumbens; Schizophrenia; TTX