title: The Mediterranean scorpion Mesobuthus gibbosus (Scorpiones, Buthidae): transcriptome analysis and organization of the genome encoding chlorotoxin-like peptides.
abstract: BACKGROUND
  Transcrof toxin genes of scorpion species have been published. Up to this moment, no information on the gene characterization of M. gibbosus is available.
  
  
  RESULTS
  This study provides the first insight into gene expression in venom glands from M. gibbosus scorpion. A cDNA library was generated from the venom glands and subsequently analyzed (301 clones). Sequences from 177 high-quality ESTs were grouped as 48 Mgib sequences, of those 48 sequences, 40 (29 "singletons" and 11 "contigs") correspond with one or more ESTs. We identified putative precursor sequences and were grouped them in different categories (39 unique transcripts, one with alternative reading frames), resulting in the identification of 12 new toxin-like and 5 antimicrobial precursors (transcripts). The analysis of the gene families revealed several new components categorized among various toxin families with effect on ion channels. Sequence analysis of a new KTx precursor provides evidence to validate a new KTx subfamily (α-KTx 27.x). A second part of this work involves the genomic organization of three Meg-chlorotoxin-like genes (ClTxs). Genomic DNA sequence reveals close similarities (presence of one same-phase intron) with the sole genomic organization of chlorotoxins ever reported (from M. martensii).
  
  
  CONCLUSIONS
  Transcriptome analysis is a powerful strategy that provides complete information of the gene expression and molecular diversity of the venom glands (telson). In this work, we generated the first catalogue of the gene expression and genomic organization of toxins from M. gibbosus. Our result represents a relevant contribution to the knowledge of toxin transcripts and complementary information related with other cell function proteins and venom peptide transcripts. The genomic organization of the chlorotoxin genes may help to understand the diversity of this gene family.
authors.forename: Elia
authors.initials: E
authors.lastname: Diego-García
authors.forename: Figen
authors.initials: F
authors.lastname: Caliskan
authors.affiliation: Toxicology and Pharmacology, University of Leuven, Campus Gasthuisberg O& N2,PO Box 922, Herestraat 49, 3000 Leuven, Belgium. jan.tytgat@pharm.kuleuven.be.
authors.forename: Jan
authors.initials: J
authors.lastname: Tytgat
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018076:DNA, Complementary; D016678:Genome; D008969:Molecular Sequence Data; D012604:Scorpion Venoms / Q000737:chemistry / Q000235:genetics*; D012605:Scorpions / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D059467:Transcriptome
chemical_list: D018076:DNA, Complementary; D012604:Scorpion Venoms; C091539:Chlorotoxin
references.citation: Toxicon. 2013 Mar 1;63:44-54
references.pmid: 23182832
references.citation: FEBS Lett. 1998 Dec 28;441(3):387-91
references.pmid: 9891977
references.citation: J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Apr 15;803(1):67-73
references.pmid: 15025999
references.citation: Comp Biochem Physiol C Toxicol Pharmacol. 2007 Jul-Aug;146(1-2):147-157
references.pmid: 17270501
references.citation: Toxicon. 2010 Dec 15;56(7):1155-61
references.pmid: 19931296
references.citation: Nucleic Acids Res. 1997 Sep 1;25(17):3389-402
references.pmid: 9254694
references.citation: Proc Natl Acad Sci U S A. 1994 Feb 15;91(4):1475-9
references.pmid: 7509073
references.citation: Biochem Biophys Res Commun. 2007 Sep 7;360(4):728-34
references.pmid: 17624312
references.citation: Toxicon. 2004 Jun 15;43(8):865-75
references.pmid: 15208019
references.citation: Trends Pharmacol Sci. 1999 Nov;20(11):444-7
references.pmid: 10542442
references.citation: Toxicon. 2013 Jan;61:72-82
references.pmid: 23142506
references.citation: Biochim Biophys Acta. 2010 Apr;1804(4):872-83
references.pmid: 20045493
references.citation: Toxicon. 2011 Apr;57(5):695-703
references.pmid: 21329713
references.citation: Peptides. 2007 Jan;28(1):31-7
references.pmid: 17141373
references.citation: Biochem Biophys Res Commun. 2013 Jan 4;430(1):113-8
references.pmid: 23200836
references.citation: Toxicon. 2005 Dec 15;46(8):831-44
references.pmid: 16274721
references.citation: Neuroscience. 1998 Apr;83(4):1161-73
references.pmid: 9502255
references.citation: Toxicon. 2007 Feb;49(2):171-80
references.pmid: 17081580
references.citation: Protein Eng Des Sel. 2004 Jan;17(1):107-12
references.pmid: 14985543
references.citation: Toxicon. 2004 Jun 15;43(8):961-71
references.pmid: 15208029
references.citation: Mol Cell Proteomics. 2012 Jan;11(1):M111.012054
references.pmid: 21969612
references.citation: J Biol Chem. 2002 Aug 16;277(33):30040-7
references.pmid: 12034709
references.citation: Nat Commun. 2013;4:2602
references.pmid: 24129506
references.citation: Mol Immunol. 2008 Feb;45(3):828-38
references.pmid: 17675235
references.citation: Biochimie. 2001 Sep;83(9):883-9
references.pmid: 11698110
references.citation: Cell Mol Life Sci. 2008 Jan;65(1):187-200
references.pmid: 18030427
references.citation: Nat Methods. 2011 Sep 29;8(10):785-6
references.pmid: 21959131
references.citation: Biochem J. 2004 Jan 1;377(Pt 1):37-49
references.pmid: 14498829
references.citation: Brief Bioinform. 2005 Jun;6(2):118-34
references.pmid: 15975222
references.citation: Trends Genet. 2005 Jun;21(6):330-2
references.pmid: 15922831
references.citation: Peptides. 2012 Jun;35(2):291-9
references.pmid: 22484288
references.citation: Toxicon. 1985;23(1):113-25
references.pmid: 3992595
references.citation: J Proteomics. 2012 Feb 16;75(5):1563-76
references.pmid: 22155128
references.citation: BMC Evol Biol. 2008 Dec 16;8:333
references.pmid: 19087317
references.citation: Am J Physiol. 1993 Feb;264(2 Pt 1):C361-9
references.pmid: 8383429
references.citation: Biochimie. 2009 Aug;91(8):1010-9
references.pmid: 19470401
references.citation: Eur J Biochem. 1998 Jun 15;254(3):468-79
references.pmid: 9688256
references.citation: PLoS One. 2012;7(8):e43331
references.pmid: 22912855
references.citation: Peptides. 2011 Apr;32(4):656-63
references.pmid: 21262299
references.citation: IUBMB Life. 2005 Jan;57(1):13-21
references.pmid: 16036557
references.citation: Nucleic Acids Res. 1996 Sep 1;24(17):3439-52
references.pmid: 8811101
references.citation: Toxicon. 2006 Jul;48(1):12-22
references.pmid: 16762386
references.citation: J Biol Chem. 1997 Feb 14;272(7):4302-9
references.pmid: 9020148
references.citation: FEBS Lett. 2006 Oct 30;580(25):5979-87
references.pmid: 17054952
references.citation: Cell Mol Life Sci. 2004 Jun;61(12):1507-19
references.pmid: 15197474
references.citation: Toxicon. 2007 Feb;49(2):142-58
references.pmid: 17087986
references.citation: Genome Res. 1999 Sep;9(9):868-77
references.pmid: 10508846
references.citation: Proteomics. 2012 Jan;12(2):313-28
references.pmid: 22121013
references.citation: Toxicon. 2007 Feb;49(2):124-41
references.pmid: 17239913
references.citation: Toxicon. 2000 May;38(5):661-8
references.pmid: 10673158
references.citation: BMC Genomics. 2010 Jul 28;11:452
references.pmid: 20663230
references.citation: Toxicon. 2007 Mar 15;49(4):452-72
references.pmid: 17215013
references.citation: Toxicon. 2006 May;47(6):628-39
references.pmid: 16551474
references.citation: Mol Ecol. 2006 Sep;15(10):2883-94
references.pmid: 16911208
references.citation: Eur J Biochem. 1999 Sep;264(2):287-300
references.pmid: 10491073
references.citation: PLoS One. 2013 Jun 20;8(6):e66486
references.pmid: 23840487