title: Salinity and temperature variations reflecting on cellular PCNA, IGF-I and II expressions, body growth and muscle cellularity of a freshwater fish larvae.
abstract: The present study assessed the influence of salinity and temperature on body growth and on muscle cellularity of Lophiosilurus alexaxdri vitelinic larvae. Slightly salted environments negatively influenced body growth of freshwater fish larvae and we observed that those conditions notably act as an environmental influencer on muscle growth and on local expression of hypertrophia and hypeplasia markers (IGFs and PCNA). Furthermore, we could see that salinity tolerance for NaCl 4gl(-)(1) diminishes with increasing temperature, evidenced by variation in body and muscle growth, and by irregular morphology of the lateral skeletal muscle of larvae. We saw that an increase of both PCNA and autocrine IGF-II are correlated to an increase in fibre numbers and fibre diameter as the temperature increases and salinity diminishes. On the other hand, autocrine IGF-I follows the opposite way to the other biological parameters assessed, increasing as salinity increases and temperature diminishes, showing that this protein did not participate in muscle cellularity, but participating in molecular/cellular repair. Therefore, slightly salted environments may provide adverse conditions that cause some obstacles to somatic growth of this species, suggesting some osmotic expenditure with a salinity increment.
authors.affiliation: Departamento de Morfologia, Instituo de Ciências Biológicas, Universidade Federal de Minas Gerais, P.O. Box 486, Antônio Carlos 6627, Belo Horizonte, MG 30161-970, Brazil.
authors.forename: Y S
authors.initials: YS
authors.lastname: Martins
authors.affiliation: Departamento de Morfologia, Instituo de Ciências Biológicas, Universidade Federal de Minas Gerais, P.O. Box 486, Antônio Carlos 6627, Belo Horizonte, MG 30161-970, Brazil.
authors.forename: R M C
authors.initials: RM
authors.lastname: Melo
authors.affiliation: Departamento de Morfologia, Instituo de Ciências Biológicas, Universidade Federal de Minas Gerais, P.O. Box 486, Antônio Carlos 6627, Belo Horizonte, MG 30161-970, Brazil.
authors.forename: P H A
authors.initials: PH
authors.lastname: Campos-Junior
authors.affiliation: Hidrobiology and Hatchery Station of Três Marias CODEVASF, P.O. Box 11, Três Marias, MG 39205-000, Brazil.
authors.forename: J C E
authors.initials: JC
authors.lastname: Santos
authors.affiliation: Laboratório de Aquacultura da Escola de Veterinária da Universidade Federal de Minas Gerais, P.O. Box 567, Antônio Carlos 6627, Belo Horizonte, MG 30123970, Brazil.
authors.forename: R K
authors.initials: RK
authors.lastname: Luz
authors.affiliation: Departamento de Morfologia, Instituo de Ciências Biológicas, Universidade Federal de Minas Gerais, P.O. Box 486, Antônio Carlos 6627, Belo Horizonte, MG 30161-970, Brazil.
authors.forename: E
authors.initials: E
authors.lastname: Rizzo
authors.affiliation: Programa de Pós-Graduação em Zoologia de Vertebrados da PUC Minas, 500, prédio 41, Belo Horizonte, MG 30525-610, Brazil. Electronic address: bazzoli@pucminas.br.
authors.forename: N
authors.initials: N
authors.lastname: Bazzoli
mesh_terms: D000818:Animals; D001699:Biometry; D002397:Catfishes / Q000033:anatomy & histology / Q000254:growth & development* / Q000378:metabolism; D029941:Fish Proteins / Q000378:metabolism; D005453:Fluorescence; D005618:Fresh Water; D007334:Insulin-Like Growth Factor I / Q000378:metabolism*; D007335:Insulin-Like Growth Factor II / Q000378:metabolism*; D007814:Larva / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D016014:Linear Models; D018485:Muscle Fibers, Skeletal / Q000166:cytology / Q000378:metabolism; D018482:Muscle, Skeletal / Q000166:cytology* / Q000378:metabolism; D025341:Principal Component Analysis; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism*; D054712:Salinity; D013696:Temperature
chemical_list: D029941:Fish Proteins; D018809:Proliferating Cell Nuclear Antigen; D007334:Insulin-Like Growth Factor I; D007335:Insulin-Like Growth Factor II
keywords: Freshwater fish larvae; IGF-I; IGF-II; PCNA; Skeletal muscle phenotype