title: Influence of sperm fertilising concentration, sperm selection method and sperm capacitation procedure on the incidence of numerical chromosomal abnormalities in IVF early bovine embryos.
abstract: The occurrence of numerical chromosomal aberrations, widely described as a major cause of mortality in in vitro-produced (IVP) embryos, has been linked to several factors. In the present study we investigated the effect of sperm fertilising concentration and semen handling (sperm selection and capacitation) before IVF on the rate of numerical chromosomal abnormalities in bovine embryos. In all, 466 IVP cattle embryos were karyotyped throughout three sequential experiments, analysing the effects of sperm fertilising concentration (0.1, 1.0 or 10×10(6) spermatozoa mL(-1)), selection method (unselected or Percoll-selected spermatozoa) and capacitation medium (bovine serum albumin (BSA), heparin or their combination). The percentage of normal (diploid) and aberrant (haploid, polyploid or aneuploid) embryos was noted in each experiment. The rate of numerical chromosomal abnormalities was mainly affected by sperm fertilising concentration (P<0.01) and, to a lesser extent, by the sperm capacitation medium (P<0.05). Polyploidy and haploidy rates were only affected by sperm fertilising concentration (P<0.05). Interestingly, the sperm selection technique used in the present study did not reduce the incidence of chromosome abnormalities in IVP cattle embryos (P>0.05). Finally, aneuploidy rates were not affected during the experiments (P>0.05), which suggests that they are not related to sperm-related factors. On the basis of these results, we conclude that sperm fertilising concentration is the 'paternal' key factor that affects the rate of numerical chromosomal abnormalities in IVP bovine embryos. By making small adjustments to fertilising protocols, the rate of cytogenetically aberrant embryos can be markedly reduced.
authors.affiliation: Meragem Research Group, Department of Genetics, University of Cordoba, Spain.
authors.forename: Sebastián
authors.initials: S
authors.lastname: Demyda-Peyrás
authors.affiliation: Department of Medicine and Animal Surgery, University of Cordoba, Spain.
authors.forename: Jesús
authors.initials: J
authors.lastname: Dorado
authors.affiliation: Department of Medicine and Animal Surgery, University of Cordoba, Spain.
authors.forename: Manuel
authors.initials: M
authors.lastname: Hidalgo
authors.affiliation: Meragem Research Group, Department of Genetics, University of Cordoba, Spain.
authors.forename: Miguel
authors.initials: M
authors.lastname: Moreno-Millán
mesh_terms: D000818:Animals; D001947:Breeding / Q000379:methods*; D002417:Cattle; D002869:Chromosome Aberrations; D005307:Fertilization in Vitro / Q000379:methods*; D006493:Heparin; D007621:Karyotyping / Q000662:veterinary; D008297:Male; D011205:Povidone; D012710:Serum Albumin, Bovine; D012822:Silicon Dioxide; D013075:Sperm Capacitation / Q000502:physiology*; D013076:Sperm Count / Q000662:veterinary*
chemical_list: D012710:Serum Albumin, Bovine; C016039:Percoll; D012822:Silicon Dioxide; D006493:Heparin; D011205:Povidone