title: Molecular cloning and expression analysis of mannose receptor in blunt snout bream (Megalobrama amblycephala).
abstract: Mannose receptor (MR) plays a significant role in innate immune responses to pathogens in vertebrates. Here we characterized the first teleost MR from Megalobrama amblycephala, named maMR and its expression patterns were investigated. The full-length maMR consists of 5,295 bp encoding a putative protein of 1,433 amino acids. The predicted amino acid sequences showed that maMR contained a signal peptide, a cysteine-rich domain, a single fibronectin type II domain, eight tandemly arranged C-type lectin-like domains, a transmembrane domain and a C-terminal cytoplasmic domain. Phylogenetic analysis revealed the highest similarity of maMR with Danio rerio MR predicted by computational analysis. The maMR-mRNAs were ubiquitously transcribed in different tissues, However the highest transcripts were observed in head kidney. Transcripts of maMR significantly increased at the late stages of embryo and continued to be at the high levels after hatching. The maMR transcripts were significantly increased in M. amblycephala after stimulation with killed Aeromonas hydrophila.
authors.affiliation: Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Liu
authors.forename: Xiaocheng
authors.initials: X
authors.lastname: Tang
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.forename: Shun
authors.initials: S
authors.lastname: Wei
authors.forename: Rui
authors.initials: R
authors.lastname: Fang
authors.forename: Wei
authors.initials: W
authors.lastname: Ji
authors.forename: Gailing
authors.initials: G
authors.lastname: Yuan
authors.forename: Nan
authors.initials: N
authors.lastname: Chen
authors.forename: Zemao
authors.initials: Z
authors.lastname: Gu
authors.forename: Xueqin
authors.initials: X
authors.lastname: Liu
authors.forename: Weimin
authors.initials: W
authors.lastname: Wang
authors.forename: Muhammad
authors.initials: M
authors.lastname: Asim
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhou
authors.forename: Li
authors.initials: L
authors.lastname: Lin
mesh_terms: D016980:Aeromonas hydrophila / Q000502:physiology; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002645:Chickens; D003001:Cloning, Molecular / Q000379:methods*; D003530:Cyprinidae / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D005393:Fish Diseases / Q000276:immunology / Q000382:microbiology; D015870:Gene Expression; D006801:Humans; D037181:Lectins, C-Type / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D051379:Mice; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D016415:Sequence Alignment
chemical_list: D037181:Lectins, C-Type; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins
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