title: [Development of a lung cancer vaccine by transfecting dendritic cells with rAAV/CEA].
abstract: OBJECTIVE
  To study the feasibility of preparing a therapeutic lung cancer vaccine by transfecting dendritic cells (DCs) with adeno-associated virus vector carrying carcino-embryonic antigen gene (rAAV/CEA).
  
  
  METHODS
  Adherent cells (monocytes) isolated from the peripheral blood of a healthy donor were infected with rAAV/CEA virus stock or pulsed with CEA peptide (control). The monocytes in both groups were induced into mature DCs with recombinant human GM-CSF, IL-4 and TNF-α. At day 7 of induction, the mature DCs were harvested and mixed with T lymphocytes. T cell proliferation stimulated by the DCs was assessed with (3)H-thymidine uptake, and the expression of IL-4, IFN-γ, CD8, CD4, CD25 and CD69 in cytotoxic T lymphocytes (CTL) was analyzed with flow cytometry. The cytotoxicity of the CTL against the target CEA-positive lung cancer A549 cells was tested by (51)Cr releasing assay.
  
  
  RESULTS
  The DCs transfected with rAAV/CEA strongly stimulated the proliferation of the T cell populations, and the induced CTL showed high expressions of CD8, CD69 and IFN-γ. The transfected DCs exhibited a high killing ability of CEA-positive lung cancer cells, and the killing showed a CEA antigen specificity and was limited by MHC I. These results suggested the ability of rAAV/CEA-transfected DCs in generating specific cellular immunity in vitro.
  
  
  CONCLUSIONS
  It is feasible to prepare therapeutic lung cancer vaccines by transfecting DCs with rAAV/CEA.
authors.affiliation: Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.E-mail: ycx6026@126.com.
authors.forename: Changxuan
authors.initials: C
authors.lastname: You
authors.forename: Xiaotao
authors.initials: X
authors.lastname: Qian
authors.forename: Yuan
authors.initials: Y
authors.lastname: He
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.forename: Paul L
authors.initials: PL
authors.lastname: Hermonat
mesh_terms: D019496:Cancer Vaccines; D002272:Carcinoembryonic Antigen / Q000235:genetics*; D002460:Cell Line; D003713:Dendritic Cells / Q000276:immunology*; D000229:Dependovirus / Q000235:genetics*; D005822:Genetic Vectors; D006801:Humans; D009000:Monocytes / Q000276:immunology; D014162:Transfection
chemical_list: D019496:Cancer Vaccines; D002272:Carcinoembryonic Antigen