title: The nature of replication of tick-borne encephalitis virus strains isolated from residents of the Russian Far East with inapparent and clinical forms of infection.
abstract: We describe the biological properties and molecular characteristics of complete genomes of 33 tick-borne encephalitis virus (TBEV) strains that induced different forms of infection, from inapparent to severe focal ones resulting in fatal outcome. Hemagglutinating activity of Oshima-like strains was higher at pH 5.8, while activity of Sofjin- and Senhzang-like strains were higher at pH 6.2 and 6.8, respectively. We determined susceptibility of porcine kidney (PK) cell cultures to these TBEV strains by cytopathic effect (CPE), plaque formation, and size of plaques. The clinical TBEV strains had higher virus titers both in tissue culture infectious dose 50(TCID50) and in plaque-forming unit (PFU) titers and larger plaques than the inapparent strains. A comparison of virus multiplication kinetics by PFU in culture fluid with kinetics of ELISA antigen and hemagglutinin accumulation suggested a different mechanism of interaction between these virus strains and PK cells at the initial stage of cell infection.
authors.affiliation: Institute of Epidemiology and Microbiology, Siberian Branch of Russian Academy of Medical Sciences, Vladivostok, Russian Federation. Electronic address: galinaleon41@gmail.com.
authors.forename: Galina N
authors.initials: GN
authors.lastname: Leonova
authors.affiliation: Institute of Epidemiology and Microbiology, Siberian Branch of Russian Academy of Medical Sciences, Vladivostok, Russian Federation.
authors.forename: Olga S
authors.initials: OS
authors.lastname: Maystrovskaya
authors.affiliation: Limnological Institute, Siberian Branch of the Russian Academy of Sciences, Irkutsk, Russian Federation.
authors.forename: Ilya G
authors.initials: IG
authors.lastname: Kondratov
authors.affiliation: Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
authors.forename: Ikuo
authors.initials: I
authors.lastname: Takashima
authors.affiliation: Limnological Institute, Siberian Branch of the Russian Academy of Sciences, Irkutsk, Russian Federation.
authors.forename: Sergei I
authors.initials: SI
authors.lastname: Belikov
mesh_terms: D000818:Animals; D058070:Asymptomatic Diseases; D016000:Cluster Analysis; D003588:Cytopathogenic Effect, Viral; D004669:Encephalitis Viruses, Tick-Borne / Q000302:isolation & purification* / Q000502:physiology*; D004675:Encephalitis, Tick-Borne / Q000821:virology*; D005202:Asia, Eastern; D016679:Genome, Viral; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D012426:Russia; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013552:Swine; D019562:Viral Load; D010948:Viral Plaque Assay; D014774:Virulence; D014779:Virus Replication
chemical_list: D012367:RNA, Viral
keywords: Hemagglutination; Molecular genetics; Replication in cells; TBEV Far East subtype; Virulence