title: Vitamin D binding protein and vitamin D in human allergen-induced endobronchial inflammation.
abstract: Allergic asthma is a chronic disease of the airways associated with airway hyperresponsiveness, a variable degree of airflow obstruction, airway remodelling and a characteristic airway inflammation. Factors of the vitamin D axis, which include vitamin D metabolites and vitamin D binding protein (VDBP), have been linked to asthma, but only few data exist about their regulation in the lung during acute allergen-induced airway inflammation. Therefore, we analysed the regulation of factors of the vitamin D axis during the early- and late-phase reaction of allergic asthma. Fifteen patients with mild allergic asthma underwent segmental allergen challenge. VDBP was analysed in bronchoalveolar lavage fluid (BALF) and serum using the enzyme-linked immunosorbent assay (ELISA) technique. 25-hydroxyvitamin D(3)[25(OH)D(3)] and 1,25-dihydroxyvitamin D(3)[1,25(OH)(2)D(3)] were analysed by a commercial laboratory using the liquid chromatography-mass spectrometry (LC/MS) technique. VDBP (median 2·3, range 0·2-7·1 μg/ml), 25(OH)D(3) (median 0·060, range < 0·002-3·210 ng/ml) and 1,25(OH)(2)D(3) (median < 0·1, range < 0·1-2·8 pg/ml) were significantly elevated in BALF 24 h but not 10 min after allergen challenge. After correction for plasma leakage using the plasma marker protein albumin, VDBP and 25(OH)D(3) were still increased significantly while 1,25(OH)(2)D(3) was not. VDBP and 25(OH)D(3) were correlated with each other and with the inflammatory response 24 h after allergen challenge. Serum concentrations of all three factors were not influenced by allergen challenge. In conclusion, we report a significant increase in VDBP and 25(OH)D(3) in human BALF 24 h after allergen challenge, suggesting a role for these factors in the asthmatic late-phase reaction.
authors.affiliation: Department of Pneumology, University of Rostock, Rostock, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Bratke
authors.forename: A
authors.initials: A
authors.lastname: Wendt
authors.forename: K
authors.initials: K
authors.lastname: Garbe
authors.forename: M
authors.initials: M
authors.lastname: Kuepper
authors.forename: P
authors.initials: P
authors.lastname: Julius
authors.forename: M
authors.initials: M
authors.lastname: Lommatzsch
authors.forename: J C
authors.initials: JC
authors.lastname: Virchow
mesh_terms: D000328:Adult; D000485:Allergens / Q000276:immunology; D001249:Asthma / Q000276:immunology*; D001798:Blood Proteins / Q000378:metabolism*; D016535:Bronchial Hyperreactivity / Q000276:immunology; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry*; D002112:Calcifediol / Q000378:metabolism*; D002117:Calcitriol / Q000378:metabolism*; D018450:Disease Progression; D005260:Female; D006801:Humans; D008297:Male; D014807:Vitamin D / Q000031:analogs & derivatives / Q000378:metabolism*; D014809:Vitamin D-Binding Protein / Q000378:metabolism*; D055815:Young Adult
chemical_list: D000485:Allergens; D001798:Blood Proteins; D014809:Vitamin D-Binding Protein; D014807:Vitamin D; D002117:Calcitriol; D002112:Calcifediol
keywords: asthma; bronchoalveolar lavage fluid; segmental allergen challenge; vitamin D; vitamin D binding protein (VDBP)
references.citation: Mol Immunol. 2007 Mar;44(9):2370-7
references.pmid: 17113648
references.citation: Immunobiology. 2014 Mar;219(3):198-207
references.pmid: 24268110
references.citation: Proc Natl Acad Sci U S A. 1991 Oct 1;88(19):8539-43
references.pmid: 1924312
references.citation: Dermatoendocrinol. 2012 Apr 1;4(2):137-45
references.pmid: 22928069
references.citation: Physiol Genomics. 2007 Apr 24;29(2):161-8
references.pmid: 17213369
references.citation: J Immunol. 2005 Oct 1;175(7):4754-60
references.pmid: 16177123
references.citation: Nat Rev Immunol. 2008 Sep;8(9):685-98
references.pmid: 19172691
references.citation: J Immunol. 2000 Mar 1;164(5):2405-11
references.pmid: 10679076
references.citation: Blood. 2005 Nov 15;106(10):3490-7
references.pmid: 16030186
references.citation: Cell. 1999 Feb 19;96(4):507-15
references.pmid: 10052453
references.citation: J Allergy Clin Immunol. 2012 Jun;129(6):1669-71
references.pmid: 22460073
references.citation: J Clin Invest. 1998 Jan 1;101(1):252-62
references.pmid: 9421488
references.citation: J Clin Endocrinol Metab. 2010 Jul;95(7):3368-76
references.pmid: 20427486
references.citation: J Clin Endocrinol Metab. 1985 Nov;61(5):969-75
references.pmid: 3840175
references.citation: J Immunol. 2012 Dec 1;189(11):5155-64
references.pmid: 23087405
references.citation: J Steroid Biochem Mol Biol. 2007 Mar;103(3-5):316-21
references.pmid: 17368179
references.citation: J Biol Chem. 2004 Dec 17;279(51):53282-7
references.pmid: 15485893
references.citation: Am J Respir Crit Care Med. 2012 Jan 15;185(2):124-32
references.pmid: 22016447
references.citation: J Steroid Biochem Mol Biol. 2010 Jul;121(1-2):221-7
references.pmid: 20302932
references.citation: Int J Mol Med. 2013 Jul;32(1):203-10
references.pmid: 23595236
references.citation: Arch Biochem Biophys. 2012 Jul 1;523(1):95-102
references.pmid: 22446158
references.citation: J Exp Med. 2003 Aug 4;198(3):455-67
references.pmid: 12900521
references.citation: Lancet. 2013 Oct 19;382(9901):1360-72
references.pmid: 24041942
references.citation: Biochemistry. 1986 Oct 21;25(21):6467-72
references.pmid: 3790535
references.citation: J Immunol. 2001 Feb 15;166(4):2688-94
references.pmid: 11160333
references.citation: Thorax. 2010 May;65(5):456-62
references.pmid: 20435872
references.citation: Proc Natl Acad Sci U S A. 2001 Nov 20;98(24):13895-900
references.pmid: 11717447
references.citation: Clin Exp Allergy. 2012 Mar;42(3):383-91
references.pmid: 22092530
references.citation: J Immunol. 2000 May 1;164(9):4443-51
references.pmid: 10779743