title: Expression of chemokine receptor-4 in bone marrow mesenchymal stem cells on experimental rat abdominal aortic aneurysms and the migration of bone marrow mesenchymal stem cells with stromal-derived factor-1.
abstract: This study investigated the expression and role of chemokine receptor-4 (CXCR4) in bone marrow mesenchymal stem cells (BMSCs) from experimental rats with abdominal aortic aneurysms (AAA) for migration of BMSCs. Sprague-Dawley rats were divided into an experimental group and control group (n = 18 each). AAA was induced with 0.75 M solution infiltrate for 30 minutes, after which the abdomen was rinsed and closed. Saline was used in place of CaCl2 in the control group. CD34 and CD29 were detected by flow cytometry, the gene and protein expression of CXCR4 were detected by real-time polymerase chain reaction and western blot, respectively. The migration of BMSCs with stromal-derived factor-1 was detected by Transwell chamber. CD34 expression was negative and CD29 expression was positive. The gene and protein expression of CXCR4 were significantly higher in experimental group than them in control group (p < 0.05), the migration ability of BMSCs from the experimental group was significantly higher than that from the control group (p < 0.05). Stromal-derived factor -1/CXCR4 can enhance the migration of BMSCs in vitro in a rat AAA model.
authors.affiliation: Department of Vascular Surgery, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangdong, China.
authors.forename: Miao-Yun
authors.initials: MY
authors.lastname: Long
authors.affiliation: Department of Vascular Surgery, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangdong, China. Electronic address: honghao_li2012@163.com.
authors.forename: Hong-Hao
authors.initials: HH
authors.lastname: Li
authors.affiliation: Department of Vascular Surgery, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangdong, China.
authors.forename: Xin-Zhi
authors.initials: XZ
authors.lastname: Pen
authors.affiliation: Department of Vascular Surgery, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangdong, China.
authors.forename: Ming-Qing
authors.initials: MQ
authors.lastname: Huang
authors.affiliation: Department of Vascular Surgery, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangdong, China.
authors.forename: Ding-Yuan
authors.initials: DY
authors.lastname: Luo
authors.affiliation: Department of Vascular Surgery, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangdong, China.
authors.forename: Pei-Shun
authors.initials: PS
authors.lastname: Wang
mesh_terms: D000818:Animals; D017544:Aortic Aneurysm, Abdominal / Q000378:metabolism* / Q000473:pathology; D015153:Blotting, Western; D001854:Bone Marrow Cells / Q000166:cytology*; D002465:Cell Movement / Q000187:drug effects*; D048430:Cell Shape; D054377:Chemokine CXCL12 / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D010641:Phenotype; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D019718:Receptors, CXCR4 / Q000235:genetics / Q000378:metabolism*
chemical_list: D054377:Chemokine CXCL12; D012333:RNA, Messenger; D019718:Receptors, CXCR4
keywords: Abdominal aortic aneurysm; Bone marrow mesenchymal stem cells chemokine receptor-4; Stromal-derived factor-1
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