title: Characterization of musclin as a new target for treatment of hypertension.
abstract: Musclin is a novel skeletal muscle-derived factor found in the signal sequence trap of mouse skeletal muscle cDNAs. Recently, it has been demonstrated that musclin is involved in the pathogenesis of spontaneously hypertensive rats (SHRs). However, it is known as a genetic hypertension model. In the present study, we aim to investigate the role of musclin in another animal model of hypertension and characterize the direct effect of musclin on vascular contraction. The results show that expression of musclin was increased in arterial tissues isolated from DOCA-salt induced hypertensive rats or the normal rats received repeated vasoconstriction with phenylephrine. Additionally, direct incubation with phenylephrine did not modify the expression of musclin in the in vitro studies. Also, the direct effect of musclin on the increase of intracellular calcium was observed in a concentration-dependent manner. These results provide the evidence to support that musclin is involved in hypertension. Thus, musclin is suitable to be considered as a novel target for treatment of hypertension.
authors.affiliation: Department of Neurosurgery, College of Medicine, Taipei Medical University and Shuang Ho Hospital, Taipei Medical University, Taipei City 10361, Taiwan.
authors.forename: Jia-Wei
authors.identifier: 0000-0002-3311-5379
authors.initials: JW
authors.lastname: Lin
authors.affiliation: Graduate Institute of Disease Prevention and Control, College of Medicine, Taipei Medical University and Shuang Ho Hospital, Taipei Medical University, Taipei City 10361, Taiwan.
authors.forename: Cheng-Chia
authors.initials: CC
authors.lastname: Tsai
authors.affiliation: Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan City 70101, Taiwan.
authors.forename: Li-Jen
authors.identifier: 0000-0001-5438-4597
authors.initials: LJ
authors.lastname: Chen
authors.affiliation: Department of Nursing, Tzu Chi College of Technology, Hualien City 97005, Taiwan.
authors.forename: Ho-Shan
authors.initials: HS
authors.lastname: Niu
authors.affiliation: Department of Neurosurgery, College of Medicine, Taipei Medical University and Shuang Ho Hospital, Taipei Medical University, Taipei City 10361, Taiwan.
authors.forename: Chen Kuei
authors.initials: CK
authors.lastname: Chang
authors.affiliation: Department of Nursing, Tzu Chi College of Technology, Hualien City 97005, Taiwan.
authors.forename: Chiang-Shan
authors.initials: CS
authors.lastname: Niu
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D064791:Desoxycorticosterone Acetate / Q000737:chemistry; D004195:Disease Models, Animal; D005786:Gene Expression Regulation; D006973:Hypertension / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D008297:Male; D009124:Muscle Proteins / Q000378:metabolism*; D010656:Phenylephrine / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D012965:Sodium Chloride / Q000737:chemistry; D014157:Transcription Factors
chemical_list: D009124:Muscle Proteins; C498450:Ostn protein, rat; D014157:Transcription Factors; D010656:Phenylephrine; D012965:Sodium Chloride; D064791:Desoxycorticosterone Acetate; D002118:Calcium
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