title: [Establishment of a rat model of subacute toxic encephalopathy induced by 1, 2-dichloroethane].
abstract: OBJECTIVE
  To establish a rat model of 1,2-dichloroethane (DCE)-induced subacute toxic encephalopathy.
  
  
  METHODS
  Sixty Sprague-Dawley rats were randomly divided into five groups: negative control, positive control, low-dose DCE (1 472 mg/m(3)), middle-dose DCE (2 550 mg/m(3)), and high-dose DCE (4 418 mg/m(3)). The three DCE groups received static inhalation of DCE 6 hours a day for 6 consecutive days. The positive control group received intraperitoneal injection of lipopolysaccharide (5 mg/kg) and were sacrificed 8 hours after injection. Blood and brain tissue were collected, followed by determination of brain water content and HE staining for pathological examination of brain tissue.
  
  
  RESULTS
  The rats in DCE groups suffered decreased body weight with increasing DCE dose (P < 0.01), and brain water content rose with increasing DCEdose. The brain water content of middle-dose DCE group (80.09 ± 0.14%) and high-dose DCE group (80.28±0.10%) increased significantly as compared with that of the negative control group (79.46±0.23%) (P < 0.001). Optical microscopy discovered loose structure and vasodilation in the brain tissue of middle-dose DCE group, indicating obvious brain edema; the high-dose DCE group and positive control group had spongiform and vacuolated brain tissues with severe vascular dilation, indicating severe brain edema.
  
  
  CONCLUSIONS
  A rat model of subacute toxic encephalopathy induced by 1, 2-dichloroethane has been successfully established.
authors.affiliation: School of Public Health, Sun Yat-set University, Guangzhou 510080, China.
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Deng
authors.affiliation: School of Public Health, Sun Yat-set University, Guangzhou 510080, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhu
authors.affiliation: School of Public Health, Sun Yat-set University, Guangzhou 510080, China.
authors.forename: Baxiong
authors.initials: B
authors.lastname: Wei
authors.affiliation: School of Public Health, Sun Yat-set University, Guangzhou 510080, China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Zhou
authors.affiliation: School of Public Health, Sun Yat-set University, Guangzhou 510080, China.
authors.forename: Yiwei
authors.initials: Y
authors.lastname: Su
authors.affiliation: School of Public Health, Sun Yat-set University, Guangzhou 510080, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Gao
authors.affiliation: School of Public Health, Sun Yat-set University, Guangzhou 510080, China. E-mail: ymliu61@163.com.
authors.forename: Yimin
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005025:Ethylene Dichlorides / Q000633:toxicity*; D008297:Male; D020258:Neurotoxicity Syndromes; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D005025:Ethylene Dichlorides; C024565:ethylene dichloride