title: Platelet and growth factor concentrations in activated platelet-rich plasma: a comparison of seven commercial separation systems.
abstract: Platelet-rich plasma (PRP) is blood plasma that has been enriched with platelets. It holds promise for clinical use in areas such as wound healing and regenerative medicine, including bone regeneration. This study characterized the composition of PRP produced by seven commercially available separation systems (JP200, GLO PRP, Magellan Autologous Platelet Separator System, KYOCERA Medical PRP Kit, SELPHYL, MyCells, and Dr. Shin's System THROMBO KIT) to evaluate the platelet, white blood cell, red blood cell, and growth factor concentrations, as well as platelet-derived growth factor-AB (PDGF-AB), transforming growth factor beta-1 (TGF-β1), and vascular endothelial growth factor (VEGF) concentrations. PRP prepared using the Magellan Autologous Platelet Separator System and the KYOCERA Medical PRP Kit contained the highest platelet concentrations. The mean PDGF-AB concentration of activated PRP was the highest from JP200, followed by the KYOCERA Medical PRP Kit, Magellan Autologous Platelet Separator System, MyCells, and GLO PRP. TGF-β1 and VEGF concentrations varied greatly among individual samples, and there was almost no significant difference among the different systems, unlike for PDGF. The SELPHYL system produced PRP with low concentrations of both platelets and growth factors. Commercial PRP separation systems vary widely, and familiarity with their individual advantages is important to extend their clinical application to a wide variety of conditions.
authors.affiliation: Department of Plastic and Reconstructive Surgery, Kansai Medical University, 2-5-1 Shin-machi, Hirakata, Osaka, 573-1010, Japan, kushidas@takii.kmu.ac.jp.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kushida
authors.forename: Natsuko
authors.initials: N
authors.lastname: Kakudo
authors.forename: Naoki
authors.initials: N
authors.lastname: Morimoto
authors.forename: Tomoya
authors.initials: T
authors.lastname: Hara
authors.forename: Takeshi
authors.initials: T
authors.lastname: Ogawa
authors.forename: Toshihito
authors.initials: T
authors.lastname: Mitsui
authors.forename: Kenji
authors.initials: K
authors.lastname: Kusumoto
mesh_terms: D000328:Adult; D001772:Blood Cell Count; D001781:Blood Component Removal / Q000379:methods*; D002498:Centrifugation; D005260:Female; D006801:Humans; D008297:Male; D010982:Platelet-Derived Growth Factor / Q000032:analysis; D053657:Platelet-Rich Plasma / Q000737:chemistry* / Q000166:cytology*; D016212:Transforming Growth Factor beta / Q000032:analysis; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis
chemical_list: D010982:Platelet-Derived Growth Factor; D016212:Transforming Growth Factor beta; D042461:Vascular Endothelial Growth Factor A; C095477:platelet-derived growth factor AB
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