title: Free amino acid profiling in the giant puffball mushroom (Calvatia gigantea) using UPLC-MS/MS.
abstract: Wild edible and medicinal mushroom, Calvatia gigantea, was quantitatively analyzed for the determination of its free amino acids using ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The concentrations of total free amino acids, essential and non-essential amino acids were 199.65 mg/100 g, 113.69 mg/100 g, and 85.96 mg/100 g in C. gigantea, respectively. This study showed that C. gigantea, so called a giant puffball mushroom, has free amino acids content. The essential amino acids: tryptophan, isoleucine, valine, phenylalanine, leucine, threonine, lysine, histidine, methionine, and the non-essential amino acids: tyrosine, 4-hyrdroxy proline, arginine, proline, glycine, serine, alanine, glutamine, glutamic acid, aspargine, aspartic acid were detected.
authors.affiliation: Muğla Sıtkı Koçman University, Research Laboratory Center, MGL Food Analysis Laboratory, Kötekli, 48000 Muğla, Turkey. Electronic address: kivrakibrahim@gmail.com.
authors.forename: İbrahim
authors.initials: İ
authors.lastname: Kıvrak
authors.affiliation: Muğla Sıtkı Koçman University, Research Laboratory Center, MGL Food Analysis Laboratory, Kötekli, 48000 Muğla, Turkey; Muğla Sıtkı Koçman University, Department of Nutrition and Dietetics, Faculty of Health Sciences, Kötekli, 48000 Muğla, Turkey. Electronic address: seydakivrak@gmail.com.
authors.forename: Şeyda
authors.initials: Ş
authors.lastname: Kıvrak
authors.affiliation: Muğla Sıtkı Koçman University, Science Faculty, Department of Chemistry, Kötekli, 48000 Muğla, Turkey. Electronic address: hmansur@mu.edu.t.
authors.forename: Mansur
authors.initials: M
authors.lastname: Harmandar
mesh_terms: D000363:Agaricales / Q000737:chemistry*; D000596:Amino Acids / Q000032:analysis*; D005971:Glutamates; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000596:Amino Acids; D005971:Glutamates
keywords: Calvatia gigantea; Free amino acids; Mushroom; UPLC–MS/MS