title: Comparison of breast milk vitamin A concentration measured in fresh milk by a rapid field assay (the iCheck FLUORO) with standard measurement of stored milk by HPLC.
abstract: Availability of rapid, point-of-contact analytical methods would facilitate the use of breast milk vitamin A concentration (BMVA) to assess vitamin A (VA) status. We compared BMVA concentrations measured by high-performance liquid chromatography (HPLC) (the standard technique) with those by iCheck FLUORO, a new portable fluorometer that can rapidly quantify BMVA. Casual breast milk samples (n=154) were collected during a representative survey in Yaoundé and Douala, Cameroon. Milk fat and BMVA concentrations (by iCheck) were measured in fresh milk in the field. After storage at <-20 °C, BMVA concentrations were also measured by HPLC. BMVA values from the two methods were highly correlated (R(2)=0.72 for BMVA/l; R(2)=0.62 for BMVA/g fat, both P<0.0001). HPLC values were greater than iCheck values on average, and the difference increased with increasing BMVA. The iCheck FLUORO could be useful for monitoring fortification programs, but before-after surveys to assess change in BMVA concentrations should use one method consistently.
authors.affiliation: Program in International and Community Nutrition, Department of Nutrition, University of California, Davis, Davis, CA, USA.
authors.forename: R
authors.initials: R
authors.lastname: Engle-Stone
authors.affiliation: Program in International and Community Nutrition, Department of Nutrition, University of California, Davis, Davis, CA, USA.
authors.forename: M J
authors.initials: MJ
authors.lastname: Haskell
authors.affiliation: USDA Western Human Nutrition Research Center, Davis, CA, USA.
authors.forename: M R
authors.initials: MR
authors.lastname: La Frano
authors.affiliation: Helen Keller International, Yaoundé, Cameroon.
authors.forename: A O
authors.initials: AO
authors.lastname: Ndjebayi
authors.affiliation: Helen Keller International, Yaoundé, Cameroon.
authors.forename: M
authors.initials: M
authors.lastname: Nankap
authors.affiliation: Program in International and Community Nutrition, Department of Nutrition, University of California, Davis, Davis, CA, USA.
authors.forename: K H
authors.initials: KH
authors.lastname: Brown
mesh_terms: D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005260:Female; D005470:Fluorometry / Q000379:methods; D006801:Humans; D007223:Infant; D008895:Milk, Human / Q000737:chemistry*; D014801:Vitamin A / Q000032:analysis*
chemical_list: D014801:Vitamin A
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