title: Induction of a laccase Lcc9 from Coprinopsis cinerea by fungal coculture and its application on indigo dye decolorization.
abstract: A fungal coculture system comprised of Coprinopsis cinerea Okayama 7 (#130) and Gongronella sp. w5 produced 900 times higher laccase activity than that in pure culture. A fungal laccase named Lcc9 was induced from C. cinerea for the first time by coculture. Lcc9 was purified, characterized, and found to have high activity toward phenolic substrates at the optimum pH of 6.5 and temperature of 60°C. The laccase was stable at alkaline pH values, and its activity was not significantly affected by cations and organic solvents. Lcc9 showed decolorization capability toward indigo dye in the presence of 2,2'-azino-bis(3-ethylbenzothazoline-6-sulfonate), with 75% of indigo was decolorized by 50 U/L enzyme after 1h of incubation under optimal catalytic conditions. These results showed that fungal coculture could active silent laccase gene, and the unusual properties make Lcc9 a candidate for specific industrial and environmental applications.
authors.affiliation: School of Life Sciences, Anhui University, Hefei, Anhui 230601, China; Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei, Anhui 230601, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Pan
authors.affiliation: School of Life Sciences, Anhui University, Hefei, Anhui 230601, China; Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei, Anhui 230601, China.
authors.forename: Nannan
authors.initials: N
authors.lastname: Zhao
authors.affiliation: School of Life Sciences, Anhui University, Hefei, Anhui 230601, China; Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei, Anhui 230601, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Yin
authors.affiliation: School of Life Sciences, Anhui University, Hefei, Anhui 230601, China; Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei, Anhui 230601, China.
authors.forename: Tianwei
authors.initials: T
authors.lastname: Zhang
authors.affiliation: School of Life Sciences, Anhui University, Hefei, Anhui 230601, China; Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei, Anhui 230601, China.
authors.forename: Xiaolan
authors.initials: X
authors.lastname: Xu
authors.affiliation: School of Life Sciences, Anhui University, Hefei, Anhui 230601, China; Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei, Anhui 230601, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Fang
authors.affiliation: Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei, Anhui 230601, China; Modern Biotechnology Center, Anhui University, Hefei, Anhui 230039, China.
authors.forename: Yuzhi
authors.initials: Y
authors.lastname: Hong
authors.affiliation: School of Life Sciences, Anhui University, Hefei, Anhui 230601, China; Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei, Anhui 230601, China. Electronic address: zemin_fang@ahu.edu.cn.
authors.forename: Zemin
authors.initials: Z
authors.lastname: Fang
authors.affiliation: School of Life Sciences, Anhui University, Hefei, Anhui 230601, China; Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei, Anhui 230601, China. Electronic address: yzxiao@ahu.edu.cn.
authors.forename: Yazhong
authors.initials: Y
authors.lastname: Xiao
mesh_terms: D001673:Biodegradation, Environmental / Q000187:drug effects; D002244:Carbon / Q000494:pharmacology; D018920:Coculture Techniques; D003116:Color; D004396:Coloring Agents / Q000378:metabolism*; D003302:Coprinus / Q000187:drug effects / Q000201:enzymology*; D004790:Enzyme Induction; D004795:Enzyme Stability / Q000187:drug effects; D005110:Extracellular Space / Q000201:enzymology; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D007203:Indigo Carmine / Q000378:metabolism*; D042845:Laccase / Q000096:biosynthesis* / Q000302:isolation & purification; D063347:Native Polyacrylamide Gel Electrophoresis; D009584:Nitrogen / Q000494:pharmacology; D009930:Organic Chemicals / Q000494:pharmacology; D012997:Solvents / Q000494:pharmacology; D013379:Substrate Specificity / Q000187:drug effects; D013696:Temperature
chemical_list: D004396:Coloring Agents; D009930:Organic Chemicals; D012997:Solvents; D002244:Carbon; D007203:Indigo Carmine; D042845:Laccase; D009584:Nitrogen
keywords: Coprinopsis cinerea; Decolorization; Fungal coculture; Gongronella sp. w5; Laccase