title: The Frequency of Mutations in Exon 11 of the c-kit Gene in Patients With Leukemia.
abstract: OBJECTIVE
  To determine the frequency of mutations in exon 11 of the c-kit gene in patients with leukemia.
  
  
  METHODS
  The study included 50 leukemia patients (31 with acute myeloid leukemia, 5 with acutelymphoblastic leukemia, 9 with chronic myeloid leukemia, and 5 with chronic lymphocytic leukemia) that underwentPCR-SSCP, followed by direct DNA sequencing.
  
  
  RESULTS
  In all, 28 of the leukemia patients were male and 22 were female, with a mean age of 31.88 years (range: 2-65years). In total, 20 mutations in 19 patients were identified, including Lys550Asn, Tyr568Ser, Ile571Thr, Thr574Pro,Gln575His, Tyr578Pro, Asp579His, His580Gln, Arg586Thr, Asn587Asp, and Arg588Met, as well as novel point mutationsat codons Ile563Lys, Val569Leu, Tyr570Ser, and Pro577Ser. Ile571Leu substitution was observed in 2 patients andTrp582Ser substitution was observed in 3 patients.
  
  
  CONCLUSIONS
  The results suggest that mutations in exon 11 of the c-kit gene might be useful as molecular geneticmarkers for leukemia.
authors.affiliation: Era's Lucknow Medical College and Hospital, Lucknow, India.
authors.forename: Syed Rizwan
authors.initials: SR
authors.lastname: Hussain
authors.affiliation: Babasaheb Bhimrao Ambedkar University, Lucknow, India.
authors.forename: Sunil G
authors.initials: SG
authors.lastname: Babu
authors.affiliation: Era's Lucknow Medical College and Hospital, Lucknow, India.
authors.forename: Hena
authors.initials: H
authors.lastname: Naqvi
authors.affiliation: Era's Lucknow Medical College and Hospital, Lucknow, India.
authors.forename: Pradyumn
authors.initials: P
authors.lastname: Singh
authors.affiliation: Era's Lucknow Medical College and Hospital, Lucknow, India.
authors.forename: Farzana
authors.initials: F
authors.lastname: Mahdi
keywords: C-kit; Lösemi; Mutasyon; SSCP
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