title: Isolation and characterization of Cr (VI) tolerant bacteria from tannery waste and its bioremediation potential.
abstract: The East Kolkata wetland, situated in West Bengal, India, is presently being exploited by housing many leather complexes surrounding the zone in and around. High amount of either untreated or partially treated liquid tannery wastes are discharged in the wetland. Treated effluent from some common effluent treatment plant (CETP) showed the presence of Cr (VI) exceeding the limiting concentration. Three different bacterial strains (L1, L2, and L3) were isolated from the treated effluent. Morphological and biochemical characterizations exhibited that they belong to Gram positive Bacillus group. All such strains proved to be chromate tolerant in the concentration ranging from 20 to 50 mg/L. Significant reduction in Cr (VI) was observed in all those strains, especially L3 [86.3% removal corresponding to 30 mg/L initial concentration]. Cytosolic fraction was involved in Cr reductase capacity. Molecular characterization by 16S rDNA genome sequencing of L3 [the most promising isolate, in terms of chromium (VI) tolerance] confirmed that the strain is Bacillus cereus GXBC-1.
authors.forename: Rupa
authors.initials: R
authors.lastname: Chakraborty
authors.forename: Arup Kumar
authors.initials: AK
authors.lastname: Mitra
authors.forename: Kumar Sinha
authors.initials: KS
authors.lastname: Roy
authors.forename: Somnath
authors.initials: S
authors.lastname: Mukherjee
authors.forename: Sunil
authors.initials: S
authors.lastname: Kumar
mesh_terms: D001409:Bacillus cereus / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism; D002857:Chromium / Q000378:metabolism*; D052918:Environmental Restoration and Remediation; D007220:Industrial Waste; D013633:Tanning; D062065:Wastewater / Q000382:microbiology*
chemical_list: D002857:Chromium; D007220:Industrial Waste; D062065:Wastewater; C074702:chromium hexavalent ion