title: An exopolysaccharide from Trichoderma pseudokoningii and its apoptotic activity on human leukemia K562 cells.
abstract: In this study, a novel exopolysaccharide (EPS) was isolated from the fermentation broth of Trichoderma pseudokoningii and its anticancer activities on human leukemia K562 cells were studied. EPS could significantly inhibited K562 cells proliferation in a time- and concentration-dependent manner. Meanwhile, characteristic of apoptosis, including apoptotic morphological features and the apoptosis rate were obtained. Sequentially, the dissipation of mitochondrial membrane potential, increase production of Reactive oxygen species (ROS), enhancement of the concentration of intracellular, up-regulation of Bax and p53 mRNA, down-regulation of Bcl-2 mRNA were also detected. The results indicate that the EPS could induce of K562 cells apoptosis, primarily in involved the mitochondrial pathways. The present studies suggest that EPS could be a new potential adjuvant chemotherapeutic and chemo preventive agent against human leukemia.
authors.affiliation: School of Life Sciences, Shandong University, Jinan 250100, PR China.
authors.forename: Taotao
authors.initials: T
authors.lastname: Huang
authors.affiliation: Department of Pharmacy, Wannan Medical college, Wuhu 241000, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Lin
authors.affiliation: School of Life Sciences, Shandong University, Jinan 250100, PR China.
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Cao
authors.affiliation: School of Life Sciences, Shandong University, Jinan 250100, PR China; National Glycoengineering Research Center, School of Life Science, Shandong University, Jinan 250100, PR China.
authors.forename: Pengying
authors.initials: P
authors.lastname: Zhang
authors.affiliation: School of Life Sciences, Shandong University, Jinan 250100, PR China.
authors.forename: Yungui
authors.initials: Y
authors.lastname: Bai
authors.affiliation: School of Life Sciences, Shandong University, Jinan 250100, PR China.
authors.forename: Guochuang
authors.initials: G
authors.lastname: Chen
authors.affiliation: School of Life Sciences, Shandong University, Jinan 250100, PR China; Department of Pharmacy, Wannan Medical college, Wuhu 241000, PR China; National Glycoengineering Research Center, School of Life Science, Shandong University, Jinan 250100, PR China.
authors.forename: Kaoshan
authors.initials: K
authors.lastname: Chen
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002118:Calcium / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D062610:Fungal Polysaccharides / Q000494:pharmacology*; D006801:Humans; D020014:K562 Cells; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D014242:Trichoderma; D016159:Tumor Suppressor Protein p53 / Q000235:genetics; D051028:bcl-2-Associated X Protein / Q000235:genetics
chemical_list: D000970:Antineoplastic Agents; C494826:BAX protein, human; D062610:Fungal Polysaccharides; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D016159:Tumor Suppressor Protein p53; D051028:bcl-2-Associated X Protein; D002118:Calcium