title: Novel integration strategy for enhancing chalcopyrite bioleaching by Acidithiobacillus sp. in a 7-L fermenter.
abstract: An integrated strategy (additional energy substrate-three stage pH control-fed batch) was firstly proposed for efficiently improving chalcopyrite bioleaching by Acidithiobacillus sp. in a 7-L fermenter. The strain adaptive-growing phase was greatly shortened from 8days into 4days with the supplement of additional 2g/L Fe(2+)+2g/L S(0). Jarosite passivation was effectively weakened basing on higher biomass via the three-stage pH-stat control (pH 1.3-1.0-0.7). The mineral substrate inhibition was attenuated by fed-batch fermentation. With the integrated strategy, the biochemical reaction was promoted and achieved a better balance. Meanwhile, the domination course of A. thiooxidans in the microbial community was shortened from 14days to 8days. As the results of integrated strategy, the final copper ion and productivity reached 89.1mg/L and 2.23mg/(Ld), respectively, which was improved by 52.8% compared to the uncontrolled batch bioleaching. The integrated strategy could be further exploited for industrial chalcopyrite bioleaching.
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, PR China.
authors.forename: Shoushuai
authors.initials: S
authors.lastname: Feng
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, PR China.
authors.forename: Hailin
authors.initials: H
authors.lastname: Yang
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, PR China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Zhan
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, PR China. Electronic address: bioprocessor@aliyun.com.
authors.forename: Wu
authors.initials: W
authors.lastname: Wang
mesh_terms: D042763:Acidithiobacillus / Q000378:metabolism*; D003300:Copper / Q000302:isolation & purification* / Q000378:metabolism; D005285:Fermentation; D005290:Ferric Compounds / Q000378:metabolism; D013431:Sulfates / Q000378:metabolism
chemical_list: D005290:Ferric Compounds; D013431:Sulfates; C492331:jarosite; C012819:chalcopyrite; D003300:Copper
keywords: Acidithiobacillus; Chalcopyrite bioleaching; Copper ion; Jarosite; Microbial community