title: Vessel specific imaging of glucose transfer with fluorescent glucose analogue in anesthetized mouse cortex.
abstract: The present study examined glucose transfer in the cellular scale of mouse brain microvasculature in vivo using two-photon microscopy and fluorescent glucose analogue (2-NBDG). The 2-NBDG was intravenously injected (0.04 mL/min) in the anesthetized Tie2-GFP mice in which the vascular endothelium expressed fluorescent protein. Time-lapse imaging was conducted on the cortical parenchyma, while the time-intensity change of the injected 2-NBDG was analysed in respective vascular compartments (artery, capillary, and vein). We observed that 2-NBDG signal increased monotonically in the vasculature during the period of the injection, and rapidly declined following its cessation. In tissue compartment, however, the signal intensity gradually increased even after cessation of the injection. Spatiotemporal analysis of the 2-NBDG intensity over the cross-sections of the vessels further showed distinct change of the 2-NBDG intensity across the vessel wall (endothelium), which may represents a regulation site of tissue glucose influx.
authors.affiliation: Department of Mechanical Engineering and Intelligent System, The University of Electro-Communications, Tokyo, Japan.
authors.forename: Rei
authors.initials: R
authors.lastname: Murata
authors.affiliation: Department of Mechanical Engineering and Intelligent System, The University of Electro-Communications, Tokyo, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Takada
authors.affiliation: Molecular Imaging Center, National Institute of Radiological Sciences, Chiba, 263-8555, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Takuwa
authors.affiliation: Molecular Imaging Center, National Institute of Radiological Sciences, Chiba, 263-8555, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kawaguchi
authors.affiliation: Molecular Imaging Center, National Institute of Radiological Sciences, Chiba, 263-8555, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ito
authors.affiliation: Molecular Imaging Center, National Institute of Radiological Sciences, Chiba, 263-8555, Japan.
authors.forename: Iwao
authors.initials: I
authors.lastname: Kanno
authors.affiliation: Department of Mechanical Engineering and Intelligent System, The University of Electro-Communications, Tokyo, Japan.
authors.forename: Naotomo
authors.initials: N
authors.lastname: Tottori
authors.affiliation: Department of Mechanical Engineering and Intelligent System, The University of Electro-Communications, Tokyo, Japan.
authors.forename: Yukio
authors.initials: Y
authors.lastname: Yamada
authors.affiliation: Department of Neurology, Keio University School of Medicine, Tokyo, Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Tomita
authors.affiliation: Department of Neurology, Keio University School of Medicine, Tokyo, Japan.
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Itoh
authors.affiliation: Department of Neurology, Keio University School of Medicine, Tokyo, Japan.
authors.forename: Norihiro
authors.initials: N
authors.lastname: Suzuki
authors.affiliation: Department of Physiology, Hirosaki University Graduate School of Medicine, Hirosaki, Japan.
authors.forename: Katsuya
authors.initials: K
authors.lastname: Yamada
authors.affiliation: Molecular Imaging Center, National Institute of Radiological Sciences, Chiba, 263-8555, Japan. masamoto@mce.uec.ac.jp.|Department of Mechanical Engineering and Intelligent System, Graduate School of Informatics and Engineering, The University of Electro-Communications, 1-5-1 Chofugaoka, Chofu, Tokyo, 182-8585, Japan. masamoto@mce.uec.ac.jp.|Brain Science Inspired Life Support Research Center, University of Electro-Communications, 1-5-1 Chofugaoka, Chofu, Tokyo, 182-8585, Japan. masamoto@mce.uec.ac.jp.
authors.forename: Kazuto
authors.initials: K
authors.lastname: Masamoto
mesh_terms: D000758:Anesthesia; D000818:Animals; D002540:Cerebral Cortex / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D051379:Mice
chemical_list: D049452:Green Fluorescent Proteins; D005947:Glucose