title: Human mesenchymal stem cells with adenovirus-mediated TRAIL gene transduction have antitumor effects on esophageal cancer cell line Eca-109.
abstract: The apoptotic ligand TNF-related apoptosis-inducing ligand (TRAIL) is believed to be a promising candidate for cancer gene therapy, yet gene therapy strategies to tackle this disease systemically are often impaired by inefficient delivery of the vector to the tumor tissue. Mesenchymal stem cells (MSCs) have been shown to home to tumor sites and could potentially act as a shield and vehicle for an antitumor gene therapy vector. Here, we used an adenoviral vector expressing TRAIL to transduce MSCs and studied the apoptosis-inducing activity of these TRAIL-carrying MSCs on esophageal cancer cell Eca-109. Our results showed that, in vitro, TRAIL-expressing MSCs were able to inhibit proliferation and induce apoptosis in Eca-109 cells by an MTT assay, co-culture experiments and flow cytometry analysis. In vivo, TRAIL-expressing MSCs also displayed an ability to inhibit tumor growth in an Eca-109 xenograft mouse model. Together, our findings indicated that the gene therapy strategy of MSCs-based TRAIL gene delivery has a wide potential value for improving the treatment of esophageal cancer.
authors.affiliation: Department of Thoracic Surgery, Qilu Hospital, Shandong University, Jinan 250012, China linlisci@163.com.
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Orthopedics, Shandong Province Hospital, Shandong University, Jinan 250021, China.
authors.forename: Fengling
authors.initials: F
authors.lastname: Li
authors.affiliation: Department of Thoracic Surgery, Qilu Hospital, Shandong University, Jinan 250012, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Tian
authors.affiliation: Department of Thoracic Surgery, Qilu Hospital, Shandong University, Jinan 250012, China.
authors.forename: Weiming
authors.initials: W
authors.lastname: Yue
authors.affiliation: Department of Thoracic Surgery, Qilu Hospital, Shandong University, Jinan 250012, China.
authors.forename: Shuhai
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Thoracic Surgery, Qilu Hospital, Shandong University, Jinan 250012, China.
authors.forename: Guanqing
authors.initials: G
authors.lastname: Chen
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D015153:Blotting, Western; D045744:Cell Line, Tumor; D018920:Coculture Techniques; D004797:Enzyme-Linked Immunosorbent Assay; D004938:Esophageal Neoplasms / Q000473:pathology*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D053221:TNF-Related Apoptosis-Inducing Ligand / Q000502:physiology*
chemical_list: D053221:TNF-Related Apoptosis-Inducing Ligand
keywords: TRAIL; esophageal cancer; gene therapy; mesenchymal stem cells