title: Artificial biomimicking matrix modifications of nanofibrous scaffolds by hE-cadherin-Fc fusion protein to promote human mesenchymal stem cells adhesion and proliferation.
abstract: Extracellular matrix (ECM) plays a fundamental role in regulating cell attachment, proliferation, migration and differentiation. Both synthetic and biologically derived materials have been explored as an ECM in regenerative medicine and tissue engineering. To biomimick the extracellular matrix, we combined the advantages of the biological properties of nanofibrous scaffolds and the fusion protein to apply for the culture of human mesenchymal stem cells in vitro. In this study, we fabricated well random-oriented/aligned nanofibrous scaffolds with PCL, modified with hE-cadherin-Fc fusion protein and studied the synergistic effect of the scaffolds. The random-oriented/aligned architecture was observed in the nanofibrous scaffolds by SEM. XPS and WCA measurements evidenced that hE-cadherin-Fc was successfully modified on the PCL nanofibrous scaffolds and hydrophilicity of the scaffolds was well improved after fusion protein coating. The hE-cadherin-Fc modified markedly promoted the adhesion and proliferation of hMSCs and guided hMSCs to a spindlier morphology compared with unmodified nanofibrous scaffolds. Furthermore, hMSCs on the hE-cadherin-Fc-coated nanofibrous scaffolds also had differentiation potential. These results suggested that the combination of PCL nanofibrous scaffolds and hE-cadherin-Fc fusion protein may be a promising artificial ECM for the behavior of hMSCs in vitro.
authors.forename: Jianbin
authors.initials: J
authors.lastname: Xu
authors.forename: Suhua
authors.initials: S
authors.lastname: Li
authors.forename: Feifei
authors.initials: F
authors.lastname: Hu
authors.forename: Chuanshun
authors.initials: C
authors.lastname: Zhu
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Akaike
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
mesh_terms: D040761:Biomimetic Materials / Q000737:chemistry / Q000493:pharmacokinetics; D015820:Cadherins / Q000737:chemistry / Q000235:genetics / Q000493:pharmacokinetics*; D002448:Cell Adhesion / Q000502:physiology; D015815:Cell Adhesion Molecules / Q000737:chemistry / Q000235:genetics / Q000493:pharmacokinetics*; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000493:pharmacokinetics; D004867:Equipment Design; D019544:Equipment Failure Analysis; D016326:Extracellular Matrix Proteins / Q000737:chemistry / Q000493:pharmacokinetics*; D006801:Humans; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology*; D011091:Polyesters / Q000737:chemistry*; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000493:pharmacokinetics; D023822:Tissue Engineering / Q000295:instrumentation; D054457:Tissue Scaffolds
chemical_list: D015820:Cadherins; D015815:Cell Adhesion Molecules; D020099:Coated Materials, Biocompatible; D016326:Extracellular Matrix Proteins; C106607:FCGBP protein, human; D011091:Polyesters; D011993:Recombinant Fusion Proteins; C016240:polycaprolactone