title: Significant population genetic structure of the Cameroonian fresh water snail, Bulinus globosus, (Gastropoda: Planorbidae) revealed by nuclear microsatellite loci analysis.
abstract: In order to characterize the demographic traits and spatial structure of Cameroonians Bulinus globosus, intermediate host of Schistosoma haematobium, genetic structure of seven different populations, collected from the tropical zone, was studied using six polymorphic microsatellites. Intrapopulation genetic diversity ranged from 0.37 to 0.55. Interpopulation genetic diversity variation clearly illustrated their significant isolation due to distance with gene flow substantially limited to neighbouring populations. The effective population sizes (Ne) were relatively low (from 3.0 to 18.6), which supposes a high rate from which populations would lose their genetic diversity by drift. Analysis of genetic temporal variability indicated fluctuations of allelic frequencies (35 of 42 locus-population combinations, P<0.05) characteristic of stochastic demography, and this is reinforced by events of bottlenecks detected in all populations. These findings demonstrated that Cameroonian B. globosus were mixed-maters with some populations showing clear preference for outcrossing. These data also suggest that genetic drift and gene flow are the main factors shaping the genetic structure of studied populations.
authors.affiliation: INSERM UMR 1094, Institute of Neuroepidemiology and Tropical Neurology, and Faculties of Medicine and Pharmacy, 2 rue du Docteur Raymond Marcland, 87025 Limoges, France; Laboratory of General Biology, Department of Biology and Animal Physiology, Faculty of Science, PO Box 812, Yaoundé, Cameroon. Electronic address: ffnouboue@yahoo.fr.
authors.forename: F F
authors.initials: FF
authors.lastname: Djuikwo-Teukeng
authors.affiliation: Animal Molecular Genetics Unit (UGMA), UMR INRA 1061, Faculty of Science and Technology, 123 Cours Albert Thomas, 87000 Limoges, France. Electronic address: anne.blondeau@unilim.fr.
authors.forename: A
authors.initials: A
authors.lastname: Da Silva
authors.affiliation: Laboratory of General Biology, Department of Biology and Animal Physiology, Faculty of Science, PO Box 812, Yaoundé, Cameroon. Electronic address: njiokouf@yahoo.com.
authors.forename: F
authors.initials: F
authors.lastname: Njiokou
authors.affiliation: Laboratory of Malaria Research (OCEAC), PO Box 288, Yaoundé, Cameroon. Electronic address: kamgang_d@yahoo.fr.
authors.forename: B
authors.initials: B
authors.lastname: Kamgang
authors.affiliation: University Hospital Centre, Department of Parasitology, PO Box 3266, Yaoundé, Cameroon. Electronic address: sameekobo@gmail.com.
authors.forename: A Same
authors.initials: AS
authors.lastname: Ekobo
authors.affiliation: INSERM UMR 1094, Institute of Neuroepidemiology and Tropical Neurology, and Faculties of Medicine and Pharmacy, 2 rue du Docteur Raymond Marcland, 87025 Limoges, France. Electronic address: gilles.dreyfuss@unilim.fr.
authors.forename: G
authors.initials: G
authors.lastname: Dreyfuss
mesh_terms: D000818:Animals; D002033:Bulinus / Q000145:classification* / Q000235:genetics*; D002163:Cameroon; D002467:Cell Nucleus / Q000235:genetics*; D005618:Fresh Water; D014644:Genetic Variation; D060005:Genotyping Techniques; D018895:Microsatellite Repeats; D058974:Phylogeography; D062211:Spatio-Temporal Analysis; D014329:Tropical Climate
keywords: Bulinus globosus; Genetic diversity; Microsatellites; Population genetic structure; Schistosoma haematobium