title: Antrodia cinnamomea profoundly exalted the reversion of activated hepatic stellate cells by the alteration of cellular proteins.
abstract: The direct modulation of Antrodia cinnamomea (AC) on the prominent role of liver fibrosis-hepatic stellate cells (HSCs) in situ remains unclear. Firstly, the administration of A. cinnamomea mycelial extract (ACME) could improve liver morphology and histological changes including collagen formation and GPT activity in the liver of thioacetamide (TAA)-injured rats. The morphology and fatty acid restore of TAA-induced HSCs (THSCs) returned to the non-chemical induced HSCs (NHSCs) type as measured by immunofluorescence and Oil Red O staining. PPARγ was upregulated associated with the lowering of α-SMA protein in NHSC-ACME. ACME inhibited the MMP-2 activity in NHSCs by gelatin Zymography. After LC-MS/MS, the cytoskeleton (tubulin, lamin A) and heat shock protein 8 in NHSC-ACME, and guanylate kinase, brain-specific kinase, SG-II and p55 proteins were downregulated in THSC-ACME. Whereas MHC class II, SMC6 protein, and phospholipase D were upregulated in NHSC-ACME. Furthermore, PKG-1 was downregulated in NHSC-ACME and upregulated in THSC-ACME. SG-II and p55 proteins were downregulated in NHSC-ACME and THSC-ACME by Western blotting. Taken together, the beneficial effect of A. cinnamomea on the induction of HSC cellular proteins is potentially applied as an alternative and complementary medicine for the prevention and amelioration of a liver injury.
authors.affiliation: Institute of Biotechnology, National Dong Hwa University, Hualien 974, Taiwan.
authors.forename: Yi-Ren
authors.initials: YR
authors.lastname: Chen
authors.affiliation: Institute of Biotechnology, National Dong Hwa University, Hualien 974, Taiwan.
authors.forename: Kai-Ting
authors.initials: KT
authors.lastname: Chang
authors.affiliation: Neural Regeneration Laboratory, Neurological Institute, Taipei Veterans General Hospital, Taipei 112, Taiwan.
authors.forename: May-Jywan
authors.initials: MJ
authors.lastname: Tsai
authors.affiliation: Institute of Biotechnology, National Dong Hwa University, Hualien 974, Taiwan.
authors.forename: Chia-Hung
authors.initials: CH
authors.lastname: Lee
authors.affiliation: Institute of Biotechnology, National Dong Hwa University, Hualien 974, Taiwan.
authors.forename: Kao-Jean
authors.initials: KJ
authors.lastname: Huang
authors.affiliation: Neural Regeneration Laboratory, Neurological Institute, Taipei Veterans General Hospital, Taipei 112, Taiwan.
authors.forename: Henrich
authors.initials: H
authors.lastname: Cheng
authors.affiliation: Department of Chemistry, National Dong Hwa University, Hualien 974, Taiwan.
authors.forename: Yen-Peng
authors.initials: YP
authors.lastname: Ho
authors.affiliation: Department of Biotechnology, Southern Taiwan University, Tainan 710, Taiwan.
authors.forename: Jian-Chyi
authors.initials: JC
authors.lastname: Chen
authors.affiliation: Department of Research, Buddhist Tzu Chi General Hospital, General Education Center, Tzu Chi College of Technology, Hualien 970, Taiwan.
authors.forename: Hsueh-Hui
authors.initials: HH
authors.lastname: Yang
authors.affiliation: Institute of Biotechnology, National Dong Hwa University, Hualien 974, Taiwan. Electronic address: cfweng@mail.ndhu.edu.tw.
authors.forename: Ching-Feng
authors.initials: CF
authors.lastname: Weng
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D055491:Antrodia / Q000737:chemistry*; D015415:Biomarkers / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D005227:Fatty Acids / Q000378:metabolism; D055951:Functional Food; D055166:Hepatic Stellate Cells / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D061965:Matrix Metalloproteinase Inhibitors / Q000494:pharmacology; D047495:PPAR gamma / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D017207:Rats, Sprague-Dawley; D013853:Thioacetamide / Q000633:toxicity
chemical_list: D000199:Actins; D015415:Biomarkers; D005227:Fatty Acids; D020778:Matrix Metalloproteinase 2; D061965:Matrix Metalloproteinase Inhibitors; D047495:PPAR gamma; D010936:Plant Extracts; D013853:Thioacetamide; C498862:Acta2 protein, rat
keywords: Antrodia cinnamomea; Hepatic stellate cells; Proteomic analysis; Reversion