title: Patellins 3 and 6, two members of the Plant Patellin family, interact with the movement protein of Alfalfa mosaic virus and interfere with viral movement.
abstract: Movement proteins (MPs) encoded by plant viruses interact with host proteins to facilitate or interfere with intra- and/or intercellular viral movement. Using yeast two-hybrid and bimolecular fluorescence complementation assays, we herein present in vivo evidence for the interaction between Alfalfa mosaic virus (AMV) MP and Arabidopsis Patellin 3 (atPATL3) and Patellin 6 (atPATL6), two proteins containing a Sec14 domain. Proteins with Sec14 domains are implicated in membrane trafficking, cytoskeleton dynamics, lipid metabolism and lipid-mediated regulatory functions. Interestingly, the overexpression of atPATL3 and/or atPATL6 interfered with the plasmodesmata targeting of AMV MP and correlated with reduced infection foci size. Consistently, the viral RNA levels increased in the single and double Arabidopsis knockout mutants for atPATL3 and atPATL6. Our results indicate that, in general, MP-PATL interactions interfere with the correct subcellular targeting of MP, thus rendering the intracellular transport of viral MP-containing complexes less efficient and diminishing cell-to-cell movement.
authors.affiliation: Instituto de Biología Molecular y Celular de Plantas (UPV-CSIC), Ingeniero Fausto Elio s/n, 46022, Valencia, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Peiro
authors.forename: Ana Cristina
authors.initials: AC
authors.lastname: Izquierdo-Garcia
authors.forename: Jesus Angel
authors.initials: JA
authors.lastname: Sanchez-Navarro
authors.forename: Vicente
authors.initials: V
authors.lastname: Pallas
authors.forename: Jose Miguel
authors.initials: JM
authors.lastname: Mulet
authors.forename: Frederic
authors.initials: F
authors.lastname: Aparicio
mesh_terms: D017794:Alfalfa mosaic virus / Q000378:metabolism*; D017360:Arabidopsis / Q000378:metabolism* / Q000821:virology*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D050556:Fatty Acid-Binding Proteins; D055786:Gene Knockout Techniques; D009068:Movement; D053830:Plant Viral Movement Proteins / Q000378:metabolism*; D031425:Plasmodesmata / Q000378:metabolism; D011485:Protein Binding; D021381:Protein Transport; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D013347:Subcellular Fractions / Q000378:metabolism; D020798:Two-Hybrid System Techniques
chemical_list: D029681:Arabidopsis Proteins; D002352:Carrier Proteins; D050556:Fatty Acid-Binding Proteins; C000599258:Patellin 3 protein, Arabidopsis; C000599259:Patellin 6 protein, Arabidopsis; D053830:Plant Viral Movement Proteins
keywords: AMV; ilarvirus; intercellular movement; movement protein; patellin
references.citation: Proc Natl Acad Sci U S A. 2010 Feb 9;107(6):2491-6
references.pmid: 20133785
references.citation: Adv Enzyme Regul. 2007;47:27-40
references.pmid: 17335879
references.citation: Biochim Biophys Acta. 2007 Jun;1771(6):727-36
references.pmid: 17512778
references.citation: Mol Plant Microbe Interact. 2007 Jun;20(6):671-81
references.pmid: 17555275
references.citation: J Gen Virol. 2003 Feb;84(Pt 2):497-505
references.pmid: 12560584
references.citation: Arch Virol. 2001;146(5):923-39
references.pmid: 11448030
references.citation: Annu Rev Phytopathol. 2005;43:39-62
references.pmid: 16078876
references.citation: Virology. 1998 May 10;244(2):322-9
references.pmid: 9601503
references.citation: Mol Plant Pathol. 2009 Mar;10(2):161-73
references.pmid: 19236566
references.citation: Mol Cells. 2004 Apr 30;17(2):223-9
references.pmid: 15179034
references.citation: Mol Cell. 2002 Apr;9(4):789-98
references.pmid: 11983170
references.citation: Chem Biol. 2004 May;11(5):681-9
references.pmid: 15157879
references.citation: Virology. 1991 Apr;181(2):445-50
references.pmid: 2014633
references.citation: Mol Plant Microbe Interact. 2001 Sep;14(9):1063-74
references.pmid: 11551071
references.citation: Nature. 1970 Aug 15;227(5259):680-5
references.pmid: 5432063
references.citation: J Plant Physiol. 2006 Nov;163(11):1150-8
references.pmid: 16542754
references.citation: Mol Cells. 2001 Aug 31;12(1):57-66
references.pmid: 11561731
references.citation: J Gen Virol. 2000 Jan;81(Pt 1):257-66
references.pmid: 10640565
references.citation: Mol Plant Microbe Interact. 2001 Sep;14(9):1051-62
references.pmid: 11551070
references.citation: Virology. 2006 Mar 1;346(1):66-73
references.pmid: 16316673
references.citation: Plant Physiol. 2003 Aug;132(4):1870-83
references.pmid: 12913144
references.citation: Virology. 2005 Aug 15;339(1):31-41
references.pmid: 15963545
references.citation: Plant Cell. 1995 Dec;7(12):2101-14
references.pmid: 8718621
references.citation: Mol Plant. 2011 Sep;4(5):782-93
references.pmid: 21746703
references.citation: Mol Plant Microbe Interact. 2012 Aug;25(8):1093-103
references.pmid: 22746826
references.citation: Plant J. 2003 Aug;35(3):386-92
references.pmid: 12887589
references.citation: J Gen Virol. 2013 Mar;94(Pt 3):677-681
references.pmid: 23136366
references.citation: Plant Physiol. 2004 Oct;136(2):3080-94; discussion 3001-2
references.pmid: 15466235
references.citation: Plant Cell. 2005 Oct;17(10):2817-31
references.pmid: 16126836
references.citation: Biochem Cell Biol. 2004 Feb;82(1):254-62
references.pmid: 15052341
references.citation: Plant Physiol. 2007 Feb;143(2):801-11
references.pmid: 17189338
references.citation: J Gen Virol. 2006 Jun;87(Pt 6):1745-1750
references.pmid: 16690941
references.citation: PLoS One. 2007 Aug 08;2(8):e718
references.pmid: 17684564
references.citation: J Gen Virol. 2010 Jul;91(Pt 7):1865-70
references.pmid: 20219894
references.citation: EMBO J. 2000 Mar 1;19(5):913-20
references.pmid: 10698933
references.citation: Plant Physiol. 2008 Jun;147(2):611-23
references.pmid: 18408045
references.citation: Plant Physiol. 2005 Aug;138(4):1866-76
references.pmid: 16006596
references.citation: Methods Enzymol. 2002;350:87-96
references.pmid: 12073338
references.citation: J Virol. 2009 Jun;83(11):5535-43
references.pmid: 19321624
references.citation: PLoS One. 2011;6(11):e27409
references.pmid: 22087310
references.citation: Genome Biol. 2002;3(5):research0023
references.pmid: 12049664
references.citation: Biochem Soc Trans. 2007 Feb;35(Pt 1):142-5
references.pmid: 17233621
references.citation: Proc Natl Acad Sci U S A. 2000 Feb 29;97(5):2373-8
references.pmid: 10688879
references.citation: Crit Rev Biochem Mol Biol. 2006 Jan-Feb;41(1):21-49
references.pmid: 16455519
references.citation: Plant Physiol. 2005 Aug;138(4):1815-21
references.pmid: 16172094
references.citation: Arch Virol. 1997;142(4):749-63
references.pmid: 9170502
references.citation: Virology. 2006 Jan 5;344(1):169-84
references.pmid: 16364748
references.citation: Mol Plant Microbe Interact. 2006 Aug;19(8):874-83
references.pmid: 16903353
references.citation: J Gen Virol. 2011 Dec;92(Pt 12):2691-2705
references.pmid: 21900418
references.citation: Arch Virol. 2009;154(6):959-67
references.pmid: 19458900
references.citation: FEBS Lett. 2002 Oct 30;531(1):74-80
references.pmid: 12401207
references.citation: Curr Opin Plant Biol. 2004 Aug;7(4):365-71
references.pmid: 15231257
references.citation: Adv Virus Res. 2013;87:139-81
references.pmid: 23809923
references.citation: J Gen Virol. 2006 Sep;87(Pt 9):2699-2707
references.pmid: 16894211
references.citation: Methods Mol Biol. 1998;81:461-8
references.pmid: 9760535
references.citation: J Exp Bot. 2002 Jun;53(373):1531-2
references.pmid: 12021302