title: LX loaded nanoliposomes synthesis, characterization and cellular uptake studies in H2O2 stressed SH-SY5Y cells.
abstract: In this study, we report the cellular uptake studies of novel LX loaded nanoliposomes in H2O2 stress SH-SY5Y Cells synthesized by thin film evaporation method. We have isolated the smallest size nanoliposomes after 90 min ultrasonification, keeping Polydisperse Index as 0.259. The morphology, size, zepta potential and drug efficiency of prepared nanoliposomes are characterized by using Transmission Electron Microscope (TEM), particle size analyzer and High Pressure Liquid Chromatography (HPLC). The particle size analyzer have confirmed the particle size of nanoluposomes measured in range of 100-250 nm, whereas the shape of these nanoliposomes is almost spherical. The zeta potential of small size nanoliposomes was measured as -49.62 and encapsulation efficiency of the LX loaded nanoliposomes was 87%. The oxidative stress response in SH-SY5Y Cells for various doses of drug with and without nanoliposomes has affectively improved the cell-stress response up to 20% after 24 h of incubation at 37 degrees C. The results indicated that LX loaded nanoliposomes were taken by the cells effectively which ultimately improved the cell-stress response. Thus, this study confirmed that synthesized nanoliposomes are not only effective drug carriers but could be potentially used for delivery of genes, antibodies, and proteins in future.
authors.forename: Murtaza
authors.initials: M
authors.lastname: Hasan
authors.forename: Javed
authors.initials: J
authors.lastname: Iqbal
authors.forename: Umer
authors.initials: U
authors.lastname: Awan
authors.forename: Nian
authors.initials: N
authors.lastname: Xin
authors.forename: Hao
authors.initials: H
authors.lastname: Dang
authors.forename: Baradi
authors.initials: B
authors.lastname: Waryani
authors.forename: Yasmeen
authors.initials: Y
authors.lastname: Saeed
authors.forename: Kaleem
authors.initials: K
authors.lastname: Ullah
authors.forename: Dai
authors.initials: D
authors.lastname: Rongji
authors.forename: Yulin
authors.initials: Y
authors.lastname: Deng
mesh_terms: D045744:Cell Line, Tumor; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage* / Q000494:pharmacology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D008657:Metabolic Clearance Rate; D053769:Nanocapsules / Q000737:chemistry* / Q000648:ultrastructure; D009447:Neuroblastoma / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects*
chemical_list: D004365:Drugs, Chinese Herbal; D053769:Nanocapsules; D006861:Hydrogen Peroxide