title: Closely related phytoplankton species produce similar suites of dissolved organic matter.
abstract: Production of dissolved organic matter (DOM) by marine phytoplankton supplies the majority of organic substrate consumed by heterotrophic bacterioplankton in the sea. This production and subsequent consumption converts a vast quantity of carbon, nitrogen, and phosphorus between organic and inorganic forms, directly impacting global cycles of these biologically important elements. Details regarding the chemical composition of DOM produced by marine phytoplankton are sparse, and while often assumed, it is not currently known if phylogenetically distinct groups of marine phytoplankton release characteristic suites of DOM. To investigate the relationship between specific phytoplankton groups and the DOM they release, hydrophobic phytoplankton-derived dissolved organic matter (DOMP) from eight axenic strains was analyzed using high-performance liquid chromatography coupled to mass spectrometry (HPLC-MS). Identification of DOM features derived from Prochlorococcus, Synechococcus, Thalassiosira, and Phaeodactylum revealed DOMP to be complex and highly strain dependent. Connections between DOMP features and the phylogenetic relatedness of these strains were identified on multiple levels of phylogenetic distance, suggesting that marine phytoplankton produce DOM that in part reflects its phylogenetic origin. Chemical information regarding the size and polarity ranges of features from defined biological sources was also obtained. Our findings reveal DOMP composition to be partially conserved among related phytoplankton species, and implicate marine DOM as a potential factor influencing microbial diversity in the sea by acting as a link between autotrophic and heterotrophic microbial community structures.
authors.affiliation: Department of Biology, Woods Hole Oceanographic Institution Woods Hole, MA, USA ; Department of Civil and Environmental Engineering, Massachusetts Institute of Technology Cambridge, MA, USA.
authors.forename: Jamie W
authors.initials: JW
authors.lastname: Becker
authors.affiliation: Department of Civil and Environmental Engineering, Massachusetts Institute of Technology Cambridge, MA, USA.
authors.forename: Paul M
authors.initials: PM
authors.lastname: Berube
authors.affiliation: Department of Marine Chemistry and Geochemistry, Woods Hole Oceanographic Institution Woods Hole, MA, USA.
authors.forename: Christopher L
authors.initials: CL
authors.lastname: Follett
authors.affiliation: Department of Biology, Woods Hole Oceanographic Institution Woods Hole, MA, USA.
authors.forename: John B
authors.initials: JB
authors.lastname: Waterbury
authors.affiliation: Department of Civil and Environmental Engineering, Massachusetts Institute of Technology Cambridge, MA, USA ; Department of Biology, Massachusetts Institute of Technology Cambridge, MA, USA.
authors.forename: Sallie W
authors.initials: SW
authors.lastname: Chisholm
authors.affiliation: Department of Civil and Environmental Engineering, Massachusetts Institute of Technology Cambridge, MA, USA ; Department of Biological Engineering, Massachusetts Institute of Technology Cambridge, MA, USA.
authors.forename: Edward F
authors.initials: EF
authors.lastname: Delong
authors.affiliation: Department of Marine Chemistry and Geochemistry, Woods Hole Oceanographic Institution Woods Hole, MA, USA.
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Repeta
keywords: Phaeodactylum; Prochlorococcus; Synechococcus; Thalassiosira; dissolved organic matter; exometabolome; marine phytoplankton; untargeted metabolomics
references.citation: Environ Microbiol. 2010 Jul;12(7):1978-88
references.pmid: 20345942
references.citation: Mol Biosyst. 2011 Dec;7(12):3200-6
references.pmid: 21935552
references.citation: Environ Microbiol. 2008 Jul;10(7):1771-82
references.pmid: 18393994
references.citation: Cladistics. 2004 Oct;20(5):487-497
references.pmid: 34892952
references.citation: BMC Evol Biol. 2011 May 14;11:125
references.pmid: 21569560
references.citation: PLoS Genet. 2007 Dec;3(12):e231
references.pmid: 18159947
references.citation: Proc Natl Acad Sci U S A. 2010 Sep 21;107(38):16420-7
references.pmid: 20807744
references.citation: Microbiol Mol Biol Rev. 1999 Mar;63(1):106-27
references.pmid: 10066832
references.citation: FEBS Lett. 1999 Dec 3;462(3):358-62
references.pmid: 10622725
references.citation: Proc Natl Acad Sci U S A. 2013 Jun 11;110(24):9824-9
references.pmid: 23703908
references.citation: Anal Bioanal Chem. 2013 Jan;405(1):109-24
references.pmid: 22965531
references.citation: Science. 2005 Aug 19;309(5738):1242-5
references.pmid: 16109880
references.citation: Appl Environ Microbiol. 2000 Apr;66(4):1692-7
references.pmid: 10742262
references.citation: J Am Soc Mass Spectrom. 2010 Sep;21(9):1471-6
references.pmid: 20452782
references.citation: Plant Physiol. 1971 Jun;47(6):841-3
references.pmid: 16657716
references.citation: Environ Microbiol. 2014 Sep;16(9):2815-30
references.pmid: 24118765
references.citation: Environ Microbiol. 2010 Mar;12(3):616-27
references.pmid: 19930445
references.citation: Environ Microbiol. 2012 Jun;14(6):1500-16
references.pmid: 22507662
references.citation: Nat Biotechnol. 2012 Sep;30(9):826-8
references.pmid: 22965049
references.citation: Nature. 2003 Aug 28;424(6952):1042-7
references.pmid: 12917642
references.citation: Environ Microbiol. 2009 Jun;11(6):1340-7
references.pmid: 19187282
references.citation: Metabolites. 2013 Feb 04;3(1):72-100
references.pmid: 24957891
references.citation: Environ Microbiol. 2012 Sep;14(9):2348-60
references.pmid: 22639946
references.citation: Proc Natl Acad Sci U S A. 2010 Oct 26;107(43):18634-9
references.pmid: 20937887
references.citation: Anal Chem. 2012 Jun 5;84(11):5035-9
references.pmid: 22533540
references.citation: Nature. 2003 Aug 28;424(6952):1037-42
references.pmid: 12917641
references.citation: Environ Microbiol. 2014 Jun;16(6):1668-81
references.pmid: 24020678
references.citation: Appl Environ Microbiol. 2002 Mar;68(3):1180-91
references.pmid: 11872466
references.citation: Appl Environ Microbiol. 2011 Nov;77(21):7490-8
references.pmid: 21742918
references.citation: BMC Bioinformatics. 2010 Jul 23;11:395
references.pmid: 20650010
references.citation: Nature. 1998 Jun 4;393(6684):464-7
references.pmid: 9624000
references.citation: ISME J. 2013 Mar;7(3):603-14
references.pmid: 23096403