title: (1)H NMR serum metabonomics for understanding metabolic dysregulation in women with idiopathic recurrent spontaneous miscarriage during implantation window.
abstract: In an attempt to find out the association of metabolic dysregulation with poor endometrial receptivity and pregnancy loss, serum metabonomic profiling of women with idiopathic recurrent spontaneous miscarriage (IRSM) is carried out and compared with fertile controls. (1)H nuclear magnetic resonance (NMR)-based metabonomics was used to obtain serum metabolic profiles of 36 women with IRSM and 28 proven fertile women during the window of implantation. The acquired data were analyzed using multivariate principal component analysis, partial least-squares-discriminant analysis, and orthogonal projection to latent structure with discriminant analysis. A clear metabolic differentiation was evident between IRSM and control samples. The distinguishing metabolites, l-lysine, l-arginine, l-glutamine, l-histidine, l-threonine, l-phenylalanine, and l-tyrosine are significantly up-regulated in IRSM as compared to controls. These altered metabolites may be involved in the molecular mechanism of exaggerated inflammatory response and vascular dysfunction associated with poor endometrial receptivity in women with IRSM. The present work proposes a vital association of metabolic dysfunction with the disease pathogenesis.
authors.affiliation: School of Medical Science and Technology, Indian Institute of Technology Kharagpur , Kharagpur Pin-721302, India.
authors.forename: Priyanka
authors.initials: P
authors.lastname: Banerjee
authors.forename: Mainak
authors.initials: M
authors.lastname: Dutta
authors.forename: Sudha
authors.initials: S
authors.lastname: Srivastava
authors.forename: Mamata
authors.initials: M
authors.lastname: Joshi
authors.forename: Baidyanath
authors.initials: B
authors.lastname: Chakravarty
authors.forename: Koel
authors.initials: K
authors.lastname: Chaudhury
mesh_terms: D000026:Abortion, Habitual / Q000097:blood*; D000596:Amino Acids / Q000097:blood; D016022:Case-Control Studies; D010064:Embryo Implantation; D005260:Female; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D055442:Metabolome; D055432:Metabolomics; D011247:Pregnancy; D025341:Principal Component Analysis
chemical_list: D000596:Amino Acids