title: High resolution imaging beyond the acoustic diffraction limit in deep tissue via ultrasound-switchable NIR fluorescence.
abstract: Fluorescence imaging in deep tissue with high spatial resolution is highly desirable because it can provide details about tissue's structural, functional, and molecular information. Unfortunately, current fluorescence imaging techniques are limited either in penetration depth (microscopy) or spatial resolution (diffuse light based imaging) as a result of strong light scattering in deep tissue. To overcome this limitation, we developed an ultrasound-switchable fluorescence (USF) imaging technique whereby ultrasound was used to switch on/off the emission of near infrared (NIR) fluorophores. We synthesized and characterized unique NIR USF contrast agents. The excellent switching properties of these agents, combined with the sensitive USF imaging system developed in this study, enabled us to image fluorescent targets in deep tissue with spatial resolution beyond the acoustic diffraction limit.
authors.affiliation: 1] Department of Bioengineering, The University of Texas at Arlington, Arlington, TX 76019, USA [2] Joint Biomedical Engineering Program, The University of Texas at Arlington and The University of Texas Southwestern Medical Center at Dallas, TX 75390, USA [3].
authors.forename: Yanbo
authors.initials: Y
authors.lastname: Pei
authors.affiliation: 1] Department of Bioengineering, The University of Texas at Arlington, Arlington, TX 76019, USA [2] Joint Biomedical Engineering Program, The University of Texas at Arlington and The University of Texas Southwestern Medical Center at Dallas, TX 75390, USA [3].
authors.forename: Ming-Yuan
authors.initials: MY
authors.lastname: Wei
authors.affiliation: 1] Department of Bioengineering, The University of Texas at Arlington, Arlington, TX 76019, USA [2] Joint Biomedical Engineering Program, The University of Texas at Arlington and The University of Texas Southwestern Medical Center at Dallas, TX 75390, USA.
authors.forename: Bingbing
authors.initials: B
authors.lastname: Cheng
authors.affiliation: 1] Department of Bioengineering, The University of Texas at Arlington, Arlington, TX 76019, USA [2] Joint Biomedical Engineering Program, The University of Texas at Arlington and The University of Texas Southwestern Medical Center at Dallas, TX 75390, USA.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: 1] Department of Bioengineering, The University of Texas at Arlington, Arlington, TX 76019, USA [2] Joint Biomedical Engineering Program, The University of Texas at Arlington and The University of Texas Southwestern Medical Center at Dallas, TX 75390, USA [3].
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Xie
authors.affiliation: 1] Department of Bioengineering, The University of Texas at Arlington, Arlington, TX 76019, USA [2] Joint Biomedical Engineering Program, The University of Texas at Arlington and The University of Texas Southwestern Medical Center at Dallas, TX 75390, USA.
authors.forename: Kytai
authors.initials: K
authors.lastname: Nguyen
authors.affiliation: 1] Department of Bioengineering, The University of Texas at Arlington, Arlington, TX 76019, USA [2] Joint Biomedical Engineering Program, The University of Texas at Arlington and The University of Texas Southwestern Medical Center at Dallas, TX 75390, USA.
authors.forename: Baohong
authors.initials: B
authors.lastname: Yuan
mesh_terms: D000818:Animals; D003287:Contrast Media; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D007259:Infrared Rays; D009132:Muscles / Q000000981:diagnostic imaging*; D053758:Nanoparticles; D061848:Optical Imaging / Q000379:methods*; D013552:Swine; D014463:Ultrasonography
chemical_list: D003287:Contrast Media; D005456:Fluorescent Dyes
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