title: Poly(glycidyl methacrylate-co-N-methylolacrylamide-co-ethylene dimethacrylate) monolith coupled to high-performance liquid chromatography for the determination of adenosine phosphates in royal jelly.
abstract: A polymer monolith microextraction method coupled with high-performance liquid chromatography was developed for the determination of adenosine triphosphate, adenosine diphosphate, and adenosine monophosphate. The monolithic column was synthesized inside fused-silica capillaries using thermal initiation free-radical polymerization with glycidyl methacrylate as the monomer, ethylene dimethacrylate as the cross-linker, cyclohexanol, and 1-dodecanol as the porogen. N-Methylolacrylamide, an important hydrophilic monomer, was incorporated into the polymerization mixture to enhance the hydrophilicity of the poly(glycidyl methacrylate-co-ethylene dimethacrylate) column. The obtained poly(glycidyl methacrylate-co-N-methylolacrylamide-co-ethylene dimethacrylate) monolith was characterized by scanning electron microscopy, Fourier-transform infrared spectra, and X-ray photoelectron spectroscopy. Optimum conditions for the preconcentration and separation of the target adenosines were also investigated. Under the optimum conditions, we obtained acceptable linearities, low limits of detection, and good relative standard deviations. The developed polymer monolith microextraction with high-performance liquid chromatography method exhibited a good performance with recovery values in the range of 76.9-104.7% when applied to the determination of the adenosines in five royal jelly samples.
authors.affiliation: College of Chemistry, Jilin University, Changchun, P. R. China.
authors.forename: Dan
authors.initials: D
authors.lastname: Liu
authors.forename: Tianbin
authors.initials: T
authors.lastname: Zhang
authors.forename: Yechun
authors.initials: Y
authors.lastname: Cheng
authors.forename: Qiong
authors.initials: Q
authors.lastname: Jia
mesh_terms: D000178:Acrylamides / Q000737:chemistry; D000227:Adenine Nucleotides / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D004852:Epoxy Compounds / Q000737:chemistry; D005227:Fatty Acids / Q000032:analysis*; D008689:Methacrylates / Q000737:chemistry; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D000099219:Royal Jelly
chemical_list: D000178:Acrylamides; D000227:Adenine Nucleotides; D004852:Epoxy Compounds; D005227:Fatty Acids; D008689:Methacrylates; D011108:Polymers; D000099219:Royal Jelly; C004919:ethylene dimethacrylate; C007870:glycidyl methacrylate; C066443:N-methylolacrylamide
keywords: Adenosine; Dual functional monomers; High-performance liquid chromatography; Polymer monolith microextraction