title: Structural investigation of a glycoprotein from gum ghatti.
abstract: The glycoprotein structure of gum ghatti was investigated. The covalent bonding between polysaccharides and proteins was firstly confirmed by high performance size exclusion chromatography (HPSEC) using refractive index (RI) and UV detectors. Partial acid hydrolysis and enzymatic degradation were also utilized. Several structural fragments such as AraHex2-HexA-HexNAc, Hex4HexNAc2, AraHex4HexNAc, Hex10HexNAc and Hex4HexNAc-Asn were identified from MALDI-TOF spectrum; using 1D and 2D NMR spectra, the linkage site of amino acids and polysaccharides was determined as N-linked (Hex)n-GlcNAc-Asn. Combined with the polysaccharide structure obtained before, a glycoprotein structure model was proposed. It was composed of 1,6-linked galactose backbone, which were attached by numerous of sugar side chains and peptide chains.
authors.affiliation: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214100, China.
authors.forename: Ji
authors.initials: J
authors.lastname: Kang
authors.affiliation: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214100, China; Guelph Food Research Centre, Agriculture and Agri-Food Canada, Guelph, Ontario N1G 5C9, Canada.
authors.forename: Steve W
authors.initials: SW
authors.lastname: Cui
authors.affiliation: Guelph Food Research Centre, Agriculture and Agri-Food Canada, Guelph, Ontario N1G 5C9, Canada.
authors.forename: Qingbin
authors.initials: Q
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214100, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
authors.affiliation: Guelph Food Research Centre, Agriculture and Agri-Food Canada, Guelph, Ontario N1G 5C9, Canada.
authors.forename: Qi
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Phillips Hydrocolloids Research Centre, Glyndwr University, Wrexham, Wales LL11 2AW, UK.
authors.forename: Glyn O
authors.initials: GO
authors.lastname: Phillips
authors.affiliation: East Cereal and Oil Research Centre, Agriculture and Agri-Food Canada, Ottawa, Ontario, Canada.
authors.forename: John
authors.initials: J
authors.lastname: Nikiforuk
mesh_terms: D000596:Amino Acids / Q000032:analysis; D066241:Carbon-13 Magnetic Resonance Spectroscopy; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D006023:Glycoproteins / Q000737:chemistry*; D006868:Hydrolysis; D053149:Plant Gums / Q000737:chemistry*; D011487:Protein Conformation; D066244:Proton Magnetic Resonance Spectroscopy; D012031:Refractometry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000596:Amino Acids; D006023:Glycoproteins; D053149:Plant Gums; C015253:gum ghatti