title: Complete nucleotide sequence of two multidrug-resistant IncR plasmids from Klebsiella pneumoniae.
abstract: We report here the complete nucleotide sequence of two IncR replicons encoding multidrug resistance determinants, including β-lactam (blaDHA-1, blaSHV-12), aminoglycoside (aphA1, strA, strB), and fluoroquinolone (qnrB4, aac6'-1b-cr) resistance genes. The plasmids have backbones that are similar to each other, including the replication and stability systems, and contain a wide variety of transposable elements carrying known antibiotic resistance genes. This study confirms the increasing clinical importance of IncR replicons as resistance gene carriers.
authors.affiliation: Laboratoire de Bactériologie, Faculté de Médecine, Université Pierre et Marie Curie, Paris, France.
authors.forename: Fabrice
authors.initials: F
authors.lastname: Compain
authors.affiliation: Génopole, Institut Pasteur, Paris, France.
authors.forename: Lionel
authors.initials: L
authors.lastname: Frangeul
authors.affiliation: Laboratoire de Bactériologie, Faculté de Médecine, Université Pierre et Marie Curie, Paris, France Laboratoire de Bactériologie-Hygiène, Hôpitaux Universitaires la Pitié Salpêtrière-Charles Foix, Assistance Publique des Hôpitaux de Paris, Paris, France.
authors.forename: Laurence
authors.initials: L
authors.lastname: Drieux
authors.affiliation: Département de Bactériologie, Hôpitaux de l'Est Parisien, Assistance Publique des Hôpitaux de Paris, Paris, France.
authors.forename: Charlotte
authors.initials: C
authors.lastname: Verdet
authors.affiliation: Microbial Evolutionary Genomics, Institut Pasteur, Paris, France CNRS, Paris, France.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Brisse
authors.affiliation: Laboratoire de Bactériologie, Faculté de Médecine, Université Pierre et Marie Curie, Paris, France Département de Bactériologie, Hôpitaux de l'Est Parisien, Assistance Publique des Hôpitaux de Paris, Paris, France.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Arlet
authors.affiliation: Laboratoire de Bactériologie, Faculté de Médecine, Université Pierre et Marie Curie, Paris, France Département de Bactériologie, Hôpitaux de l'Est Parisien, Assistance Publique des Hôpitaux de Paris, Paris, France dominique.decre@upmc.fr.
authors.forename: Dominique
authors.initials: D
authors.lastname: Decré
mesh_terms: D000617:Aminoglycosides / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001483:Base Sequence; D004251:DNA Transposable Elements / Q000235:genetics*; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D024841:Fluoroquinolones / Q000494:pharmacology; D019450:Genes, MDR / Q000235:genetics*; D007711:Klebsiella pneumoniae / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D011815:R Factors / Q000235:genetics*; D017422:Sequence Analysis, DNA; D018440:beta-Lactam Resistance / Q000235:genetics; D001618:beta-Lactamases / Q000235:genetics
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D004251:DNA Transposable Elements; D024841:Fluoroquinolones; D001618:beta-Lactamases
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