title: Yeast metabolomics: sample preparation for a GC/MS-based analysis.
abstract: Metabolome sample preparation is one of the key factors in metabolomics analyses. The quality of the metabolome data will depend on the suitability of the experimental procedures to the cellular system (e.g., yeast cells) and the analytical performance. Here, we summarize a protocol for metabolome analysis of yeast cells using gas chromatography-mass spectrometry (GC-MS). First, the main phases of a metabolomics analysis are identified: sample preparation, metabolite extraction, and analysis. We also provide an overview on different methods used to quench samples and extract intracellular metabolites from yeast cells. This protocol provides a detailed description of a GC-MS-based analysis of yeast metabolome, in particular for metabolites containing amino and/or carboxyl groups, which represent most of the compounds participating in the central carbon metabolism.
authors.affiliation: IBB-Institute for Biotechnology and Bioengineering, Center of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057, Braga, Portugal, soniacarneiro@deb.uminho.pt.
authors.forename: Sónia
authors.initials: S
authors.lastname: Carneiro
authors.forename: Rui
authors.initials: R
authors.lastname: Pereira
authors.forename: Isabel
authors.initials: I
authors.lastname: Rocha
mesh_terms: D053000:Analytic Sample Preparation Methods / Q000379:methods*; D046508:Culture Techniques; D005561:Formates / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D042541:Intracellular Space / Q000378:metabolism; D055432:Metabolomics / Q000379:methods*; D000432:Methanol / Q000737:chemistry; D015003:Yeasts / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*
chemical_list: D005561:Formates; C083354:chlorocarbonic acid; D000432:Methanol