title: Solution NMR studies reveal no global flexibility in the catalytic domain of CDC25B.
abstract: The CDC25B phosphatase is a critical regulator of the cell cycle and has been validated as an important therapeutic target in cancer. Previous studies using molecular dynamics simulations have concluded that the catalytic domain of CDC25B may experience a significant degree of dynamics or be partially disordered in solution, a finding that has a pronounced impact on the structure-based development of CDC25B inhibitors. We have probed the backbone dynamics of the CDC25B catalytic domain in solution using NMR relaxation experiments and found that the core of the protein is relatively rigid and does not experience any large-scale dynamics over a broad range of time scales. Furthermore, based on residual dipolar coupling measurements we have concluded that the conformation in solution is very similar to that observed in the crystal form. Importantly, these findings rationalize the application of the CDC25B crystal structure in structure-based drug development.
authors.affiliation: Department of Pathology, University of Michigan, Ann Arbor, Michigan.
authors.forename: George
authors.initials: G
authors.lastname: Lund
authors.forename: Tomasz
authors.initials: T
authors.lastname: Cierpicki
mesh_terms: D020134:Catalytic Domain; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D011487:Protein Conformation; D012996:Solutions; D020687:cdc25 Phosphatases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D012996:Solutions; C517008:CDC25B protein, human; D020687:cdc25 Phosphatases
keywords: CDC25B; NMR dynamics; RDC; model-free analysis; protein dynamics; protein structure
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