title: Hydrodynamic delivery of IL-28B (IFN-λ3) gene ameliorates lung inflammation induced by cigarette smoke exposure in mice.
abstract: Cigarette smoke (CS) is the principal cause of pulmonary inflammatory response. IL-28 (IFN-λ) is a novel group of class II cytokines targeting the epithelial cells and IL-28 responses prominent in lungs can exert important immunomodulatory effects. We tested the hypothesis that IL-28B may modulate the lung inflammation induced by CS. Groups of mice were exposed to CS two times per day for 11 consecutive days. CS exposure induced lymphocyte, neutrophil and macrophage infiltration and inflammatory cytokine (IL-1β, tumor necrosis factor-α (TNF)-α, IL-17, and IL-4) in the airways. More importantly, all these CS-induced pathogenic changes were significantly inhibited by hydrodynamic delivery of plasmid DNA encoding mouse IL-28B. Thus, our results suggest that IL-28 cytokines are beneficial for the suppression of CS-mediated airway inflammation and may be a therapeutic target in CS-related diseases.
authors.affiliation: Zhejiang Provincial Key Laboratory of Pathophysiology, Department of Immunology, Ningbo University School of Medicine, Ningbo 315211, China; Institute of Inflammation and Immune Diseases, Shantou University Medical College, Shantou 515041, China.
authors.forename: Chuan
authors.initials: C
authors.lastname: Qiu
authors.affiliation: Zhejiang Provincial Key Laboratory of Pathophysiology, Department of Immunology, Ningbo University School of Medicine, Ningbo 315211, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Zhejiang Provincial Key Laboratory of Pathophysiology, Department of Immunology, Ningbo University School of Medicine, Ningbo 315211, China.
authors.forename: Mi
authors.initials: M
authors.lastname: Zhou
authors.affiliation: Zhejiang Provincial Key Laboratory of Pathophysiology, Department of Immunology, Ningbo University School of Medicine, Ningbo 315211, China.
authors.forename: Jianfa
authors.initials: J
authors.lastname: Liu
authors.affiliation: Institute of Inflammation and Immune Diseases, Shantou University Medical College, Shantou 515041, China.
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.affiliation: Institute of Inflammation and Immune Diseases, Shantou University Medical College, Shantou 515041, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Zhejiang Provincial Key Laboratory of Pathophysiology, Department of Immunology, Ningbo University School of Medicine, Ningbo 315211, China; Institute of Inflammation and Immune Diseases, Shantou University Medical College, Shantou 515041, China; Institute of Infection, Immunity and Inflammation, University of Glasgow, Glasgow G12 8TA, UK. Electronic address: damo.xu@glasgow.ac.uk.
authors.forename: Damo
authors.initials: D
authors.lastname: Xu
authors.affiliation: Zhejiang Provincial Key Laboratory of Pathophysiology, Department of Immunology, Ningbo University School of Medicine, Ningbo 315211, China; Institute of Inflammation and Immune Diseases, Shantou University Medical College, Shantou 515041, China. Electronic address: mingcaili@gmail.com.
authors.forename: Mingcai
authors.initials: M
authors.lastname: Li
mesh_terms: D061605:Administration, Intravenous; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D004247:DNA / Q000008:administration & dosage; D004781:Environmental Exposure; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods*; D057446:Hydrodynamics; D007372:Interferons; D007378:Interleukins / Q000032:analysis / Q000097:blood / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D010957:Plasmids / Q000008:administration & dosage; D011014:Pneumonia / Q000209:etiology* / Q000473:pathology / Q000628:therapy*; D014028:Tobacco Smoke Pollution; D000095022:Interferon Lambda
chemical_list: D004247:DNA; D007372:Interferons; D007378:Interleukins; D014028:Tobacco Smoke Pollution; C000624353:interferon-lambda, human; D000095022:Interferon Lambda
keywords: Cigarette smoke; IL-28B (IFN-λ3); Lung inflammation