title: Characterization and comparison of 5 platelet-rich plasma preparations in a single-donor model.
abstract: OBJECTIVE
  The purpose of this study was to compare the biological characteristics of platelet-rich plasma (PRP) obtained from 4 medical devices and a preparation developed in our laboratory using a single-donor model.
  
  
  METHODS
  Ten healthy persons donated blood that was processed to produce PRP by use of 4 commercial preparation systems and a protocol developed in our laboratory. Volumes and platelet, white blood cell (WBC), and red blood cell concentrations were recorded. The platelet activation status was assessed by flow cytometry. Enzyme-linked immunosorbent assay was used to determine the concentrations of vascular endothelial growth factor, platelet-derived growth factor AB, epidermal growth factor, and transforming growth factor β1. We calculated platelet capture efficiency, relative composition, and increase factors from whole blood in platelets and WBC, as well as platelet and growth factor (GF) doses, provided from each preparation.
  
  
  RESULTS
  Leukocyte-rich PRP was obtained with RegenPRP (RegenLab, Le Mont-sur-Lausanne, Switzerland) and the Mini GPS III System (Biomet Biology, Warsaw, IN) and provides PRP with higher proportions of red blood cells, WBCs, and neutrophils than leukocyte-poor PRP obtained with the Selphyl System (Selphyl, Bethlehem, PA), Arthrex ACP (Arthrex, Naples, FL), and the preparation developed in our laboratory. The highest platelet and GF concentrations and doses were obtained with the Mini GPS III System and the preparation developed in our laboratory. Different centrifugation protocols did not show differences in the percentages of activated platelets. Finally, a positive correlation between platelet doses and all the GFs studied was found, whereas a positive correlation between WBC doses and GFs was found only for vascular endothelial growth factor and epidermal growth factor.
  
  
  CONCLUSIONS
  In a single-donor model, significant biological variations in PRP obtained from different preparation systems were highlighted. The observed differences suggest different results for treated tissue and could explain the large variability in the clinical benefit of PRP reported in the literature.
  
  
  CONCLUSIONS
  Our findings will help clinicians to choose a system that meets their specific needs for a given indication.
authors.affiliation: Cell Culture and Therapy Laboratory, Hôpital de la Conception, AP-HM, Aix-Marseille University, Marseille, France. Electronic address: jeremy.magalon@nck.aphp.fr.
authors.forename: Jeremy
authors.initials: J
authors.lastname: Magalon
authors.affiliation: Cell Culture and Therapy Laboratory, Hôpital de la Conception, AP-HM, Aix-Marseille University, Marseille, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Bausset
authors.affiliation: Cell Culture and Therapy Laboratory, Hôpital de la Conception, AP-HM, Aix-Marseille University, Marseille, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Serratrice
authors.affiliation: Cell Culture and Therapy Laboratory, Hôpital de la Conception, AP-HM, Aix-Marseille University, Marseille, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Giraudo
authors.affiliation: Cell Culture and Therapy Laboratory, Hôpital de la Conception, AP-HM, Aix-Marseille University, Marseille, France.
authors.forename: Houssein
authors.initials: H
authors.lastname: Aboudou
authors.affiliation: Cell Culture and Therapy Laboratory, Hôpital de la Conception, AP-HM, Aix-Marseille University, Marseille, France.
authors.forename: Julie
authors.initials: J
authors.lastname: Veran
authors.affiliation: Cell Culture and Therapy Laboratory, Hôpital de la Conception, AP-HM, Aix-Marseille University, Marseille, France.
authors.forename: Guy
authors.initials: G
authors.lastname: Magalon
authors.affiliation: Hematology Laboratory, Hôpital de la Conception, AP-HM, Aix-Marseille University, Marseille, France.
authors.forename: Françoise
authors.initials: F
authors.lastname: Dignat-Georges
authors.affiliation: Cell Culture and Therapy Laboratory, Hôpital de la Conception, AP-HM, Aix-Marseille University, Marseille, France; Hematology Laboratory, Hôpital de la Conception, AP-HM, Aix-Marseille University, Marseille, France.
authors.forename: Florence
authors.initials: F
authors.lastname: Sabatier
mesh_terms: D000328:Adult; D000704:Analysis of Variance; D004797:Enzyme-Linked Immunosorbent Assay; D004906:Erythrocyte Count; D005260:Female; D005434:Flow Cytometry; D064368:Healthy Volunteers; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D007958:Leukocyte Count; D007962:Leukocytes / Q000166:cytology; D008297:Male; D008875:Middle Aged; D015539:Platelet Activation; D010976:Platelet Count; D010982:Platelet-Derived Growth Factor / Q000378:metabolism; D053657:Platelet-Rich Plasma / Q000166:cytology* / Q000378:metabolism*; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D055815:Young Adult
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D010982:Platelet-Derived Growth Factor; D053773:Transforming Growth Factor beta1; D042461:Vascular Endothelial Growth Factor A; C095477:platelet-derived growth factor AB