title: Genetic characterization of Curraleiro Pé-Duro bovine breed from a conservation herd of Brazilian semiarid.
abstract: Curraleiro Pé-Duro is a rustic bovine taurine breed found in Northeast of Brazil; this breed has decreased its production potentially in order to adapt to the region environment conditions. Consequently, it is under risk of extinction and is maintained at a preservation center in Piauí State, Brazil, as a source of genetic material adapted to local conditions. We analyzed genetic variability of this breed using microsatellite markers. Sixty animals were genotyped using 11 microsatellite loci normally used for paternity tests in bovines. The observed number of alleles ranged from 5 to 9, and the effective number of alleles ranged from 2.01 to 4.64. The Shannon index ranged from 0.949 to 1.669. The expected heterozygosity ranged from 0.510 to 0.798. Polymorphism information content values ranged from 0.453 to 0.751. Divergence from Hardy-Weinberg equilibrium was significant and the mean FIS value was 0.010. We conclude that this breed still has some genetic diversity, but with evident risk due to genetic drift caused by current breeding management. It will be necessary to insert animals from other herds to obtain the desired level of genetic variability in this breed remnant.
authors.affiliation: Instituto de Ciências Agrárias, Universidade Federal Rural da Amazônia, Belém, PA, Brasil.
authors.forename: E
authors.initials: E
authors.lastname: Silva Filho
authors.affiliation: Instituto de Ciências Agrárias, Universidade Federal Rural da Amazônia, Belém, PA, Brasil.
authors.forename: M H
authors.initials: MH
authors.lastname: Silva
authors.affiliation: Departamento de Zootecnia, Universidade Federal do Piauí, Campus Universitário Ministro Petrônio Portela, Teresina, PI, Brasil.
authors.forename: J E G
authors.initials: JE
authors.lastname: Campelo
authors.affiliation: Colorado Renewable Energy Society, Lakewood, CO, USA.
authors.forename: M R
authors.initials: MR
authors.lastname: Derosia
authors.affiliation: Departmento de Ciências Naturais, Universidade Federal do Pará, Breves, PA, Brasil.
authors.forename: L M L
authors.initials: LM
authors.lastname: Pinheiro
authors.affiliation: Embrapa Meio-Norte, Teresina, PI, Brasil.
authors.forename: M J O
authors.initials: MJ
authors.lastname: Almeida
mesh_terms: D000483:Alleles; D000818:Animals; D001938:Brazil; D001947:Breeding; D002417:Cattle; D056426:Genetic Loci; D014644:Genetic Variation; D005838:Genotype; D018895:Microsatellite Repeats