title: [Effect of exogenous hydrogen sulfide on BACE-1 enzyme expression and β-amyloid peptide metabolism in high-glucose primary neuronal culture].
abstract: OBJECTIVE
  To investigate the effects of exogenous hydrogen sulfide (H2S) on β-site APP cleaving enzyme 1 (BACE-1) and β-amyloid peptide (Aβ) metabolism in primary culture of neurons under high-glucose condition.
  
  
  METHODS
  The cortical neurons in primary culture under normal and high glucose (60 mmol/L) conditions for 24 h were exposed to 25, 50 and 100 µmol/L NaHS. Aβ1-42 concentration in the cell culture was measured by ELISA, and BACE-1 mRNA and protein levels were detected by fluorescent quantitative real-time PCR and Western blotting, respectively.
  
  
  RESULTS
  Compared with the neurons cultured in normal glucose, the neurons exposed to high glucose showed significantly increased Aβ1-42 concentration and BACE-1 mRNA and protein expressions (P<0.05). Exposure to 25, 50 and 100 µmol/L NaHS significantly decreased Aβ1-42 concentration and BACE-1 mRNA and protein expressions in the high-glucose cell culture (P<0.05).
  
  
  CONCLUSIONS
  Neurons exposed to high glucose exhibit increased Aβ1-42 levels and BACE-1 mRNA and protein expressions, which can be concentration-dependently decreased by NaHS.
authors.affiliation: Department of Neurology, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.E-mail: zhulijuan66@163.com.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Zhu
authors.forename: Xiaoshan
authors.initials: X
authors.lastname: Chen
authors.forename: Xuanli
authors.initials: X
authors.lastname: He
authors.forename: Yunwen
authors.initials: Y
authors.lastname: Qi
authors.forename: Yong
authors.initials: Y
authors.lastname: Yan
mesh_terms: D053829:Amyloid Precursor Protein Secretases / Q000378:metabolism*; D016229:Amyloid beta-Peptides / Q000378:metabolism*; D000818:Animals; D016282:Aspartic Acid Endopeptidases / Q000378:metabolism*; D002478:Cells, Cultured; D003470:Culture Media / Q000737:chemistry; D005947:Glucose / Q000737:chemistry; D006862:Hydrogen Sulfide / Q000494:pharmacology*; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism*; D010446:Peptide Fragments / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D016229:Amyloid beta-Peptides; D003470:Culture Media; D010446:Peptide Fragments; C075222:amyloid beta-protein (1-42); D053829:Amyloid Precursor Protein Secretases; D016282:Aspartic Acid Endopeptidases; C509281:Bace1 protein, rat; D005947:Glucose; D006862:Hydrogen Sulfide