title: The histone demethylase JMJD1A induces cell migration and invasion by up-regulating the expression of the long noncoding RNA MALAT1.
abstract: Patients with neuroblastoma due to N-Myc oncogene amplification have a high frequency of tumor metastasis. However, it is not clear how N-Myc induces cell migration, invasion and metastasis. The histone demethylase JMJD1A activates gene transcription by demethylating the lysine 9 residue of histone H3 (H3K9) at target gene promoters. The long noncoding RNA MALAT1 induces lung cancer cell migration and plays a pivotal role in lung cancer metastasis. Here we demonstrated that N-Myc up-regulated the expression of JMJD1A in N-Myc oncogene-amplified human neuroblastoma cells by directly binding to the JMJD1A gene promoter. Affymetrix microarray studies revealed that the gene second most significantly up-regulated by JMJD1A was MALAT1. Consistent with this finding, RT-PCR and chromatin immunoprecipitation assays showed that JMJD1A bound to the MALAT1 gene promoter and demethylated histone H3K9 at the MALAT1 gene promoter. Moreover, JMJD1A and MALAT1 induced, while the small molecule JMJD1A inhibitor DMOG suppressed, neuroblastoma cell migration and invasion. Taken together, our data identify a novel pathway through which N-Myc causes neuroblastoma cell migration and invasion, and provide important evidence for further development of more potent JMJD1A/MALAT1 inhibitors for the prevention of tumor metastasis.
authors.affiliation: Children's Cancer Institute Australia for Medical Research, Randwick, Sydney, Australia.
authors.forename: Andrew E
authors.initials: AE
authors.lastname: Tee
authors.forename: Dora
authors.initials: D
authors.lastname: Ling
authors.forename: Charlotte
authors.initials: C
authors.lastname: Nelson
authors.forename: Bernard
authors.initials: B
authors.lastname: Atmadibrata
authors.forename: Marcel E
authors.initials: ME
authors.lastname: Dinger
authors.forename: Ning
authors.initials: N
authors.lastname: Xu
authors.forename: Tamio
authors.initials: T
authors.lastname: Mizukami
authors.forename: Pei Y
authors.initials: PY
authors.lastname: Liu
authors.forename: Bing
authors.initials: B
authors.lastname: Liu
authors.forename: Belamy
authors.initials: B
authors.lastname: Cheung
authors.forename: Eddy
authors.initials: E
authors.lastname: Pasquier
authors.forename: Michelle
authors.initials: M
authors.lastname: Haber
authors.forename: Murray D
authors.initials: MD
authors.lastname: Norris
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Suzuki
authors.forename: Glenn M
authors.initials: GM
authors.lastname: Marshall
authors.forename: Tao
authors.initials: T
authors.lastname: Liu
mesh_terms: D000600:Amino Acids, Dicarboxylic / Q000494:pharmacology; D002461:Cell Line, Transformed; D002465:Cell Movement / Q000187:drug effects*; D024721:E-Box Elements; D015870:Gene Expression / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic; D006657:Histones / Q000378:metabolism; D006801:Humans; D056484:Jumonji Domain-Containing Histone Demethylases / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D009447:Neuroblastoma / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000378:metabolism*; D062085:RNA, Long Noncoding / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics; D014158:Transcription, Genetic; D014162:Transfection; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D000600:Amino Acids, Dicarboxylic; D006657:Histones; C557041:MALAT1 long non-coding RNA, human; D016271:Proto-Oncogene Proteins c-myc; D062085:RNA, Long Noncoding; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D056484:Jumonji Domain-Containing Histone Demethylases; C530247:KDM3A protein, human; C040947:oxalylglycine
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