title: Sarconesiopsis magellanica (Diptera: Calliphoridae) excretions and secretions have potent antibacterial activity.
abstract: The most important mechanism for combating infection using larval therapy depends on larval excretions and secretions (ES). The present work was aimed at evaluating Sarconesiopsis magellanica (Diptera: Calliphoridae) ES antibacterial activity in six bacterial strains (three Gram-positive and three Gram-negative) and comparing this to the effect of Lucilia sericata-derived ES. Antibacterial activity at 50μg/mL minimum inhibitory concentration (MIC) was observed for Staphylococcus epidermidis ATCC-12228 and Staphylococcus aureus ATCC-29213 strains, when the turbidimetry test involving S. magellanica ES was used; the rest of the bacterial strains (Staphylococcus aureus ATCC-6538, Pseudomonas aeruginosa ATCC-10145, Pseudomonas aeruginosa ATCC-9027 and Pseudomonas aeruginosa ATCC-27853) were inhibited at a 100μg/mL MIC. Twice the amount was required to inhibit the aforementioned bacteria with L. sericata-derived ES using this same technique; a similar trend was observed when the agar diffusion method was used instead. Furthermore, when the previously established MIC for each bacterial strain was used, their colonies became reduced following 1-6h incubation with S. magellanica derived ES, whilst the reduction occurred from 2 to 6hours with those from L. sericata. Although the MIC for each strain obtained with ciprofloxacin was lower than those established when using either blowfly derived-ES, the gradual reduction of the colonies occurred at a longer incubation time (6h or more). The results showed that S. magellanica ES antibacterial activity was more potent and effective, compared to that of L. sericata-derived ES.
authors.affiliation: Medical and Forensic Entomology Research Group, School of Medicine and Health Sciences, Universidad del Rosario, Colombia. Electronic address: adr186@hotmail.com.
authors.forename: Andrea
authors.initials: A
authors.lastname: Díaz-Roa
authors.affiliation: Microbiology Laboratory, Faculty of Natural and Mathematical Sciences, Universidad del Rosario, Colombia. Electronic address: maria.gaona@urosario.edu.co.
authors.forename: María A
authors.initials: MA
authors.lastname: Gaona
authors.affiliation: Medical and Forensic Entomology Research Group, School of Medicine and Health Sciences, Universidad del Rosario, Colombia. Electronic address: alexandrasegura@yahoo.es.
authors.forename: Nydia A
authors.initials: NA
authors.lastname: Segura
authors.affiliation: Molecular Biology and Immunology Department, Fundación Instituto de Inmunología de Colombia, Colombia. Electronic address: dianacarolina.suarezruiz@gmail.com.
authors.forename: Diana
authors.initials: D
authors.lastname: Suárez
authors.affiliation: Molecular Biology and Immunology Department, Fundación Instituto de Inmunología de Colombia, Colombia; Basic Sciences Department, School of Medicine and Health Sciences, Universidad del Rosario, Colombia. Electronic address: mapatarr.fidic@gmail.com.
authors.forename: Manuel A
authors.initials: MA
authors.lastname: Patarroyo
authors.affiliation: Medical and Forensic Entomology Research Group, School of Medicine and Health Sciences, Universidad del Rosario, Colombia. Electronic address: felio.bello@urosario.edu.co.
authors.forename: Felio J
authors.initials: FJ
authors.lastname: Bello
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D012634:Bodily Secretions / Q000737:chemistry / Q000378:metabolism*; D004175:Diptera / Q000378:metabolism*; D019476:Insect Proteins / Q000737:chemistry / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D013210:Staphylococcus / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D019476:Insect Proteins
keywords: Antibacterial activity; Excretions and secretions; Larval therapy; Minimum inhibitory concentration; Sarconesiopsis magellanica