title: 3D non-woven polyvinylidene fluoride scaffolds: fibre cross section and texturizing patterns have impact on growth of mesenchymal stromal cells.
abstract: Several applications in tissue engineering require transplantation of cells embedded in appropriate biomaterial scaffolds. Such structures may consist of 3D non-woven fibrous materials whereas little is known about the impact of mesh size, pore architecture and fibre morphology on cellular behavior. In this study, we have developed polyvinylidene fluoride (PVDF) non-woven scaffolds with round, trilobal, or snowflake fibre cross section and different fibre crimp patterns (10, 16, or 28 needles per inch). Human mesenchymal stromal cells (MSCs) from adipose tissue were seeded in parallel on these scaffolds and their growth was compared. Initial cell adhesion during the seeding procedure was higher on non-wovens with round fibres than on those with snowflake or trilobal cross sections. All PVDF non-woven fabrics facilitated cell growth over a time course of 15 days. Interestingly, proliferation was significantly higher on non-wovens with round or trilobal fibres as compared to those with snowflake profile. Furthermore, proliferation increased in a wider, less dense network. Scanning electron microscopy (SEM) revealed that the MSCs aligned along the fibres and formed cellular layers spanning over the pores. 3D PVDF non-woven scaffolds support growth of MSCs, however fibre morphology and mesh size are relevant: proliferation is enhanced by round fibre cross sections and in rather wide-meshed scaffolds.
authors.affiliation: Stem Cell Biology and Cellular Engineering, Helmholtz-Institute for Biomedical Engineering, RWTH Aachen University Medical School, Aachen, Germany.
authors.forename: Anne
authors.initials: A
authors.lastname: Schellenberg
authors.affiliation: Institute for Textile Technology RWTH Aachen University, Aachen, Germany.
authors.forename: Robin
authors.initials: R
authors.lastname: Ross
authors.affiliation: Stem Cell Biology and Cellular Engineering, Helmholtz-Institute for Biomedical Engineering, RWTH Aachen University Medical School, Aachen, Germany.
authors.forename: Giulio
authors.initials: G
authors.lastname: Abagnale
authors.affiliation: Stem Cell Biology and Cellular Engineering, Helmholtz-Institute for Biomedical Engineering, RWTH Aachen University Medical School, Aachen, Germany.
authors.forename: Sylvia
authors.initials: S
authors.lastname: Joussen
authors.affiliation: Institute for Textile Technology RWTH Aachen University, Aachen, Germany.
authors.forename: Philipp
authors.initials: P
authors.lastname: Schuster
authors.affiliation: Institute for Textile Technology RWTH Aachen University, Aachen, Germany.
authors.forename: Annahit
authors.initials: A
authors.lastname: Arshi
authors.affiliation: Department of Plastic and Reconstructive Surgery, Hand Surgery, Burn Center, RWTH Aachen University, Aachen, Germany.
authors.forename: Norbert
authors.initials: N
authors.lastname: Pallua
authors.affiliation: Institute for Textile Technology RWTH Aachen University, Aachen, Germany; Department of Applied Medical Engineering, Helmholtz-Institute for Biomedical Engineering, RWTH Aachen University, Aachen, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Jockenhoevel
authors.affiliation: Institute for Textile Technology RWTH Aachen University, Aachen, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Gries
authors.affiliation: Stem Cell Biology and Cellular Engineering, Helmholtz-Institute for Biomedical Engineering, RWTH Aachen University Medical School, Aachen, Germany.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Wagner
mesh_terms: D050156:Adipogenesis / Q000187:drug effects; D001672:Biocompatible Materials / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D011145:Polyvinyls / Q000494:pharmacology*; D016062:Porosity; D054457:Tissue Scaffolds / Q000737:chemistry*; D005465:Fluorocarbon Polymers
chemical_list: D001672:Biocompatible Materials; D011145:Polyvinyls; C024865:polyvinylidene fluoride; D005465:Fluorocarbon Polymers
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