title: Expression profiling and ontology analysis of long noncoding RNAs in post-ischemic heart and their implied roles in ischemia/reperfusion injury.
abstract: Long noncoding RNAs (lncRNAs) play important regulatory roles in cellular physiology. The contributions of lncRNAs to ischemic heart disease remain largely unknown. The aim of this study was to investigate the profile of myocardial lncRNAs and their potential roles at early stage of reperfusion. lncRNAs and mRNAs were profiled by microarray and the expression of some highly-dysregulated lncRNAs was further validated using polymerase chain reaction. Our results revealed that 64 lncRNAs were up-regulated and 87 down-regulated, while 50 mRNAs were up-regulated and 60 down-regulated in infarct region at all reperfusion sampled. Gene ontology analysis indicated that dysregulated transcripts were associated with immune response, spermine catabolic process, taxis, chemotaxis, polyamine catabolic process, spermine metabolic process, chemokine activity and chemokine receptor binding. Target gene-related pathway analysis showed significant changes in cytokine-cytokine receptor interaction, the chemokine signaling pathway and nucleotide oligomerization domain (NOD)-like receptor signaling pathway which have a close relationship with myocardial ischemia/reperfusion injury (MI/RI). Besides, a gene co-expression network was constructed to identify correlated targets of 10 highly-dysregulated lncRNAs. These lncRNAs may play their roles by this network in post-ischemic heart. Such results provide a foundation for understanding the roles and mechanisms of myocardial lncRNAs at early stage of reperfusion.
authors.affiliation: The Key Laboratory of Myocardial Ischemia, Harbin Medical University, Ministry of Education, Heilongjiang Province, China; Department of Cardiology, The Second Affiliated Hospital of Harbin Medical University, Harbin, PR China.
authors.forename: Youbin
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Cardiology, The Fourth Affiliated Hospital of Harbin Medical University, Harbin, PR China.
authors.forename: Guangnan
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Nutrition and Food Hygiene, Public Health College, Harbin Medical University, Harbin, PR China.
authors.forename: Huimin
authors.initials: H
authors.lastname: Lu
authors.affiliation: Department of Cardiology, The Fourth Affiliated Hospital of Harbin Medical University, Harbin, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: The Key Laboratory of Myocardial Ischemia, Harbin Medical University, Ministry of Education, Heilongjiang Province, China; Department of Cardiology, The Second Affiliated Hospital of Harbin Medical University, Harbin, PR China.
authors.forename: Xianglu
authors.initials: X
authors.lastname: Li
authors.affiliation: The Key Laboratory of Myocardial Ischemia, Harbin Medical University, Ministry of Education, Heilongjiang Province, China; Department of Cardiology, The Second Affiliated Hospital of Harbin Medical University, Harbin, PR China.
authors.forename: Huimin
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Pharmacology, Harbin Medical University, Harbin, PR China.
authors.forename: Xingda
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Pharmacology, Harbin Medical University, Harbin, PR China.
authors.forename: Tianyu
authors.initials: T
authors.lastname: Li
authors.affiliation: The Key Laboratory of Myocardial Ischemia, Harbin Medical University, Ministry of Education, Heilongjiang Province, China; Department of Cardiology, The Second Affiliated Hospital of Harbin Medical University, Harbin, PR China. Electronic address: yubodr@163.com.
authors.forename: Bo
authors.initials: B
authors.lastname: Yu
mesh_terms: D000818:Animals; D020869:Gene Expression Profiling; D063990:Gene Ontology; D053263:Gene Regulatory Networks; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D017202:Myocardial Ischemia / Q000235:genetics* / Q000378:metabolism; D015428:Myocardial Reperfusion Injury / Q000235:genetics* / Q000473:pathology; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D020411:Oligonucleotide Array Sequence Analysis; D062085:RNA, Long Noncoding / Q000235:genetics* / Q000378:metabolism
chemical_list: D062085:RNA, Long Noncoding
keywords: Heart; Ischemia; Long noncoding RNA; Reperfusion