title: Transcobalamin II (TCN2 67A>G and TCN2 776C>G) and transcobalamin II receptor (TCblR 1104C>T) polymorphisms in Korean patients with idiopathic recurrent spontaneous abortion.
abstract: OBJECTIVE
  The transcobalamin II (TCN2) 776C>G polymorphism has been reported to be a genetic risk factor for idiopathic recurrent spontaneous abortion (RSA). However, the sample size in previous studies was small, and other TCN2 polymorphisms have not been studied. Moreover, the TCN2 67A>G and 776C>G polymorphisms, and the transcobalamin II receptor (TCblR/CD320) 1104C>T polymorphism, have demonstrated associations with immune responses.
  
  
  METHODS
  Three hundred and seventy-eight RSA patients who had at least two consecutive spontaneous abortions were enrolled. Two hundred and seven control subjects were collected from a convenience sample. Polymerase chain reaction and restriction fragment length polymorphism analysis were performed to identify the TCN2 67A>G and 776C>G polymorphisms, and the TCblR 1104C>T polymorphism.
  
  
  RESULTS
  RSA patients showed significantly different frequencies of the TCN2 67AG+GG genotypes compared with control subjects.
  
  
  CONCLUSIONS
  The TCN2 67G allele is a possible risk factor for idiopathic RSA.
authors.affiliation: Institute for Clinical Research, CHA Bundang Medical Center, CHA University, Seongnam, South Korea.
authors.forename: Hyun Seok
authors.initials: HS
authors.lastname: Kim
authors.forename: Bo Eun
authors.initials: BE
authors.lastname: Lee
authors.forename: Young Joo
authors.initials: YJ
authors.lastname: Jeon
authors.forename: HyungChul
authors.initials: H
authors.lastname: Rah
authors.forename: Woo Sik
authors.initials: WS
authors.lastname: Lee
authors.forename: Ji Eun
authors.initials: JE
authors.lastname: Shin
authors.forename: Dong Hee
authors.initials: DH
authors.lastname: Choi
authors.forename: Nam Keun
authors.initials: NK
authors.lastname: Kim
mesh_terms: D000022:Abortion, Spontaneous / Q000097:blood / Q000235:genetics*; D000328:Adult; D044466:Asian People / Q000235:genetics*; D002784:Cholesterol / Q000097:blood; D005260:Female; D005492:Folic Acid / Q000097:blood; D005838:Genotype; D006710:Homocysteine / Q000097:blood; D006801:Humans; D011110:Polymorphism, Genetic; D011956:Receptors, Cell Surface / Q000235:genetics*; D014155:Transcobalamins / Q000235:genetics*; D014527:Uric Acid / Q000097:blood
chemical_list: D011956:Receptors, Cell Surface; D014155:Transcobalamins; C028176:transcobalamin receptor; D006710:Homocysteine; D014527:Uric Acid; D005492:Folic Acid; D002784:Cholesterol
keywords: Polymorphism; TCN2; TCblR; recurrent spontaneous abortion; transcobalamin II; transcobalamin II receptor