title: Selection and characterization of single-chain recombinant antibodies against infectious haematopoietic necrosis virus from mouse phage display library.
abstract: Six single-chain fragment variable (scFv) antibodies against infectious haematopoietic necrosis virus (IHNV) were selected from an antibody phage display library by phage display technology. The soluble scFv antibodies showed a molecular weight 32kDa by Western blot. Dot blot analysis revealed that the six scFv antibodies could recognize IHNV. For enzyme linked immunosorbent assay (ELISA), four scFv antibodies (P1A4, P1A12, P1D5 and P3E2) showed cross-reactivity with spring viraemia of carp virus (SVCV). However, none of the six scFv antibodies had cross-reaction with Pike fry rhabdovirus (PFRV), Soft-shelled turtle iridovirus (STIV), viral haemorrhagic septicemia virus (VHSV), or viral nervous necrosis virus (VNNV). Indirect immunofluorescence results showed that all of these scFv antibodies reacted positively with virus in the IHNV-infected cells. These scFv antibodies will be useful in diagnostic test development and pathogenesis studies for IHNV.
authors.affiliation: College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.affiliation: Shenzhen Entry-Exit Inspection and Quarantine Bureau, Shenzhen 518045, China.
authors.forename: Xiaocong
authors.initials: X
authors.lastname: Zheng
authors.affiliation: Shenzhen Entry-Exit Inspection and Quarantine Bureau, Shenzhen 518045, China.
authors.forename: Xiujie
authors.initials: X
authors.lastname: Shi
authors.affiliation: Shenzhen Entry-Exit Inspection and Quarantine Bureau, Shenzhen 518045, China.
authors.forename: Li
authors.initials: L
authors.lastname: Yu
authors.affiliation: Shenzhen Entry-Exit Inspection and Quarantine Bureau, Shenzhen 518045, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Jia
authors.affiliation: Shenzhen Entry-Exit Inspection and Quarantine Bureau, Shenzhen 518045, China.
authors.forename: Jinjin
authors.initials: J
authors.lastname: Wang
authors.affiliation: Shenzhen Entry-Exit Inspection and Quarantine Bureau, Shenzhen 518045, China.
authors.forename: Junqiang
authors.initials: J
authors.lastname: He
authors.affiliation: Shenzhen Entry-Exit Inspection and Quarantine Bureau, Shenzhen 518045, China.
authors.forename: Wensheng
authors.initials: W
authors.lastname: Lan
authors.affiliation: Shenzhen Entry-Exit Inspection and Quarantine Bureau, Shenzhen 518045, China. Electronic address: liuhong@szciq.gov.cn.
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.affiliation: College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: wuzhixin@mail.hzau.edu.cn.
authors.forename: Zhixin
authors.initials: Z
authors.lastname: Wu
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology* / Q000302:isolation & purification; D061505:Cell Surface Display Techniques / Q000662:veterinary; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005393:Fish Diseases / Q000175:diagnosis* / Q000821:virology; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D029142:Infectious hematopoietic necrosis virus / Q000276:immunology* / Q000302:isolation & purification; D051379:Mice; D011933:Reagent Kits, Diagnostic; D018353:Rhabdoviridae Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D012474:Salmon; D057127:Single-Chain Antibodies / Q000276:immunology* / Q000302:isolation & purification; D014337:Trout
chemical_list: D000914:Antibodies, Viral; D011933:Reagent Kits, Diagnostic; D057127:Single-Chain Antibodies
keywords: Infectious haematopoietic necrosis virus; Phage display; Single-chain fragment variable antibody