title: Evolutionary engineering of yeast.
abstract: Evolutionary engineering is an inverse metabolic engineering strategy which is based on increasing genetic diversity and screening large populations for desired phenotypes. This strategy is highly advantageous in certain situations over rational metabolic engineering approaches, since there is little or no requirement of detailed genetic background information for the trait of interest. Here, we describe the experimental methodology for selecting stress-resistant yeast strains via evolutionary engineering approach by either serial batch or chemostat cultivations.
authors.affiliation: Department of Molecular Biology and Genetics, Faculty of Science and Letters, Istanbul Technical University, 34469, Maslak, Istanbul, Turkey.
authors.forename: Ceren
authors.initials: C
authors.lastname: Alkım
authors.forename: Burcu
authors.initials: B
authors.lastname: Turanlı-Yıldız
authors.forename: Z Petek
authors.initials: ZP
authors.lastname: Cakar
mesh_terms: D019143:Evolution, Molecular; D005818:Genetic Engineering / Q000379:methods*; D016296:Mutagenesis; D009154:Mutation; D010641:Phenotype; D012441:Saccharomyces cerevisiae / Q000235:genetics*