title: [Alteration of apoptosis and Akt/mTOR signal pathway in hippocampal neurons of rat with post-traumatic stress].
abstract: OBJECTIVE
  To observe the changes of apoptosis and protein kinase B/the mammalian target of Rapamycin (Akt/mTOR) signal pathway in hippocampal neurons of rat with post-straumatic stress disorder (PTSD), and to investigate the mechanism of PTSD.
  
  
  METHODS
  Sixty male adult SD rats were divided into control group (n = 10) and PTSD (n = 50) model group. The PTSD animal model was established by giving the rats single-prolonged stress followed a single inescapable electric foot shock (SPS & S). The neuronal apoptosis of hiappocampus of PTSD rats at 1 d, 4 d, 7 d, 14 d and 28 d after model established was detected by flow cytometry (FCM). The expressions of phosphatase and tensin homology deleted on chromosome Ten (PTEN), phosphorylation of ARt and mTOR (p-Akt and p-mTOR) protein were detected by Western blotting.
  
  
  RESULTS
  The apoptotic cell rate in PTSD 1 d, 4 d, 7 d and 14 d rats were higher than that in control rats (P < 0.05). The PTEN expression level was higher since PTSD 1 d than that in control group, and peaked in PTSD 4 d (P < 0.05). The p-Akt expression level was lower in PTSD 1 d than that in control group, and then increased in various time points after PTSD, but it was still lower in PTSD 28 d (P < 0.05). The p-mTOR expression level was lower than that in control group since PTSD 4 d, and then increased in various time points after PTSD 4 d, but it was still lower in PTSD 28 d (P < 0.05).
  
  
  CONCLUSIONS
  The Akt/mTOR signal pathway was actived in hippocampal neurons of PTSD rats, and which was involved in neuronal apoptosis regulation.
authors.forename: Zhu-Xin
authors.initials: ZX
authors.lastname: Sui
authors.forename: Hao
authors.initials: H
authors.lastname: Liu
authors.forename: Hai-Tao
authors.initials: HT
authors.lastname: Wang
authors.forename: Zhan-Ling
authors.initials: ZL
authors.lastname: Yang
authors.forename: Ji-Gang
authors.initials: JG
authors.lastname: Liu
authors.forename: Ai-Jun
authors.initials: AJ
authors.lastname: Xu
authors.forename: He-Xiang
authors.initials: HX
authors.lastname: Lu
mesh_terms: D000818:Animals; D017209:Apoptosis; D004195:Disease Models, Animal; D006624:Hippocampus / Q000166:cytology; D008297:Male; D009474:Neurons / Q000166:cytology* / Q000473:pathology; D051059:PTEN Phosphohydrolase / Q000378:metabolism; D010766:Phosphorylation; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction; D013313:Stress Disorders, Post-Traumatic / Q000503:physiopathology*; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism*
chemical_list: C546845:mTOR protein, rat; D051057:Proto-Oncogene Proteins c-akt; D058570:TOR Serine-Threonine Kinases; D051059:PTEN Phosphohydrolase; C487216:Pten protein, rat