title: Nematode infection among ruminants in monsoon climate (Ban-Lahanam, Lao PDR) and its role as food-borne zoonosis.
abstract: Trichostrongylids infection has gained significant public health importance since Trichostrongylus spp. infections have been reported in humans in Lao PDR. In this study, gastrointestinal nematodes were identified and the intensity of infections was determined in goats and cattle, which are animals greatly used for meat production in Lahanam Village, Lao PDR. The total number of goats and bovines was 23 and 29, respectively, pertaining to 32 households surveyed in the area. Feacal samples were randomly collected from 14 goats and 11 bovines. Ninety three percent (13/14) of goats and 36% (3/11) of cattle were infected, with an average of 1,728 and 86 eggs per gram of faeces (EPG), respectively. Coproculture showed Trichostrongylus spp. (goats 16%; bovines 48%), Haemonchus spp. (goats 69%; bovines 37%), Cooperia spp. (bovines 8%) and Oesophagostomum spp. (goats 15%; bovines 6%). After performing the necropsy on an adult goat, Trichuris spp. was also found. We confirmed the presence of Oesophagostomum spp., H. contortus and T. colubriformis by morphology and DNA sequencing analysis of the ITS region of rDNA. Due to interactions between humans and goats in Lahanam Village and high EPG results, the diagnosis of species and the intensity of gastrointestinal nematode infection in these animals are important public-health issues. Other ruminant parasites, such as Oesophagostomum and Haemonchus, found in caprines and bovines, are reported to be causes of zoonosis and their presence in humans should be investigated in future field surveys in this area.
authors.affiliation: Escola de Medicina, Universidade Federal do Tocantins ? UFT, Palmas, TO, Brasil.
authors.forename: Marcello Otake
authors.initials: MO
authors.lastname: Sato
authors.affiliation: School of Health Sciences, Faculty of Medicine, Niigata University, Japan.
authors.forename: Megumi
authors.initials: M
authors.lastname: Sato
authors.affiliation: Department of Helminthology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
authors.forename: Kittipong
authors.initials: K
authors.lastname: Chaisiri
authors.affiliation: Department of Helminthology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
authors.forename: Wanna
authors.initials: W
authors.lastname: Maipanich
authors.affiliation: Department of Helminthology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
authors.forename: Tippayarat
authors.initials: T
authors.lastname: Yoonuan
authors.affiliation: Department of Helminthology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
authors.forename: Surapol
authors.initials: S
authors.lastname: Sanguankiat
authors.affiliation: Station of Malariology, Parasitology & Entomology, Savannakhet Province, Lao PDR.
authors.forename: Tiengkham
authors.initials: T
authors.lastname: Pongvongsa
authors.affiliation: National Institute of Public Health, Ministry of Health, Lao PDR.
authors.forename: Boungnong
authors.initials: B
authors.lastname: Boupha
authors.affiliation: Research Institute for Humanity & Nature, Kyoto, Japan.
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Moji
authors.affiliation: Department of Helminthology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
authors.forename: Jitra
authors.initials: J
authors.lastname: Waikagul
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology*; D002980:Climate; D005517:Foodborne Diseases / Q000469:parasitology*; D015511:Goat Diseases / Q000469:parasitology*; D006041:Goats; D007812:Laos; D009349:Nematode Infections / Q000662:veterinary*; D015047:Zoonoses