title: Design, characterization and applications of new ionic liquid matrices for multifunctional analysis of biomolecules: a novel strategy for pathogenic bacteria biosensing.
abstract: The design, preparation and performance for novel UV-light absorbing (room-temperature) ionic liquid matrices (UV-RTILMs) for matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS) were reported. A series of UV-RTILMs was prepared by ultrasonication of equimolar of acid (mefenamic acid) and bases (aniline (ANI), pyridine (Pyr), dimethyl aniline (DMANI) and 2-methyl picoline (2-P)). The UV-RTILMs have not only significant absorbance at the desired wavelength (337 nm of the N2 Laser), but also have available protons that can easily undergo proton transfer reactions to ionize the target molecules. The novel UV-RTILMs have the ability to ionize different and wide classes of compounds such as drugs, carbohydrate, and amino acids. The new UV-RTILMs series have been successfully and selectively applied for biosensing the lysates of pathogenic bacteria in the presence of the cell macromolecules. A new strategy for biosensing pathogens was presented via sensing the pathogens lysate in the cell suspension. The new materials can effectively detect the bacterial toxins without separation or any pretreatment. They offered excellent ionization of labile oligosaccharides with protonated peaks. They could significantly enhance the analyte signals, produce homogeneous spotting, reducing spot-to-spot variation, excellent vacuum stability, higher ion peak intensity, and wide application possibility. The physical parameters such as molar refractivity, molar volume, parachor, surface tension, density and polarizability were calculated and tabulated. The new UV-RTILMs could offer excellent reproducibility and great repeatability and they are promising matrices for wide applications on MALDI-MS.
authors.affiliation: Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, 804, Taiwan; Department of Chemistry, Assuit University, Assuit, 71515, Egypt.
authors.forename: Hani Nasser
authors.initials: HN
authors.lastname: Abdelhamid
authors.affiliation: Doctoral Degree Program in Marine Biotechnology, National Sun Yat-Sen University, Kaohsiung, 804, Taiwan.
authors.forename: M Shahnawaz
authors.initials: MS
authors.lastname: Khan
authors.affiliation: Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, 804, Taiwan; School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, 800, Taiwan; Institute of Medical Science and Technology, National Sun Yat-Sen University, Kaohsiung, 804, Taiwan; Doctoral Degree Program in Marine Biotechnology, National Sun Yat-Sen University, Kaohsiung, 804, Taiwan; Center for Nanoscience and Nanotechnology, National Sun Yat-Sen University, Kaohsiung, 804, Taiwan. Electronic address: hwu@faculty.nsysu.edu.tw.
authors.forename: Hui-Fen
authors.initials: HF
authors.lastname: Wu
mesh_terms: D000814:Aniline Compounds / Q000032:analysis; D015374:Biosensing Techniques; D004867:Equipment Design; D052578:Ionic Liquids / Q000737:chemistry*; D008528:Mefenamic Acid / Q000032:analysis; D010847:Picolines / Q000032:analysis; D011550:Pseudomonas aeruginosa / Q000737:chemistry* / Q000302:isolation & purification*; D011725:Pyridines / Q000032:analysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation; D013211:Staphylococcus aureus / Q000737:chemistry* / Q000302:isolation & purification*; D014466:Ultraviolet Rays
chemical_list: D000814:Aniline Compounds; D052578:Ionic Liquids; D010847:Picolines; D011725:Pyridines; D008528:Mefenamic Acid; C023666:pyridine; C023650:aniline
keywords: Biosensing; Carbohydrate; Ionic liquid matrices; Mass spectrometry; Pathogenic bacteria