title: Apigenin and Wogonin Regulate Epidermal Growth Factor Receptor Signaling Pathway Involved in MUC5AC Mucin Gene Expression and Production from Cultured Airway Epithelial Cells.
abstract: BACKGROUND
  We investigated whether wogonin and apigenin significantly affect the epidermal growth factor receptor (EGFR) signaling pathway involved in MUC5AC mucin gene expression, and production from cultured airway epithelial cells; this was based on our previous report that apigenin and wogonin suppressed MUC5AC mucin gene expression and production from human airway epithelial cells.
  
  
  METHODS
  Confluent NCI-H292 cells were pretreated with wogonin or apigenin for 15 minutes or 24 hours and then stimulated with epidermal growth factor (EGF) for 24 hours or the indicated periods.
  
  
  RESULTS
  We found that incubation of NCI-H292 cells with wogonin or apigenin inhibited the phosphorylation of EGFR. The downstream signals of EGFR such as phosphorylation of MEK1/2 and ERK1/2 were also inhibited by wogonin or apigenin.
  
  
  CONCLUSIONS
  The results suggest that wogonin and apigenin inhibits EGFR signaling pathway, which may explain how they inhibit MUC5AC mucin gene expression and production induced by EGF.
authors.affiliation: Department of Pharmacology, Chungnam National University School of Medicine, Daejeon, Korea.
authors.forename: Md Asaduzzaman
authors.initials: MA
authors.lastname: Sikder
authors.affiliation: Department of Pharmacology, Chungnam National University School of Medicine, Daejeon, Korea.
authors.forename: Hyun Jae
authors.initials: HJ
authors.lastname: Lee
authors.affiliation: Department of Pharmacology, Chungnam National University School of Medicine, Daejeon, Korea.
authors.forename: Jiho
authors.initials: J
authors.lastname: Ryu
authors.affiliation: Department of Pharmacology, Chungnam National University School of Medicine, Daejeon, Korea.
authors.forename: Su Hyun
authors.initials: SH
authors.lastname: Park
authors.affiliation: Pulmonology Section, Department of Internal Medicine, Chungnam National University Hospital, Chungnam National University School of Medicine, Daejeon, Korea.
authors.forename: Ju-Ock
authors.initials: JO
authors.lastname: Kim
authors.affiliation: Department of Pharmacology, Chungnam National University School of Medicine, Daejeon, Korea.
authors.forename: Jang-Hee
authors.initials: JH
authors.lastname: Hong
authors.affiliation: Department of Pharmacology, Chungnam National University School of Medicine, Daejeon, Korea.
authors.forename: Jeong Ho
authors.initials: JH
authors.lastname: Seok
authors.affiliation: Department of Pharmacology, Chungnam National University School of Medicine, Daejeon, Korea.
authors.forename: Choong Jae
authors.initials: CJ
authors.lastname: Lee
keywords: Apigenin; Mucins; Receptor, Epidermal Growth Factor; Wogonin
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