title: Binding affinity of ssDNA is improved by attachment of dsDNA regions.
abstract: LidNA, a microRNA inhibitor consisting of a microRNA binding ssDNA region sandwiched between dsDNA regions had higher affinity to target oligonucleotides than that without dsDNA region. This enhancement in affinity was found to be owing to the suppressed mobility of ssDNA region by the presence of dsDNA regions.
authors.affiliation: Department of Bioengineering, Graduate School of Engineering, Osaka City University, Sugimoto 3-3-138, Sumiyoshi-ku, Osaka 558-8585, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Ida
authors.affiliation: Department of Bioengineering, Graduate School of Engineering, Osaka City University, Sugimoto 3-3-138, Sumiyoshi-ku, Osaka 558-8585, Japan. Electronic address: tatibana@bioa.eng.osaka-cu.ac.jp.
authors.forename: Akira
authors.initials: A
authors.lastname: Tachibana
authors.affiliation: Department of Bioengineering, Graduate School of Engineering, Osaka City University, Sugimoto 3-3-138, Sumiyoshi-ku, Osaka 558-8585, Japan.
authors.forename: Toshizumi
authors.initials: T
authors.lastname: Tanabe
mesh_terms: D020029:Base Pairing; D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000737:chemistry*; D015342:DNA Probes / Q000737:chemistry*; D004277:DNA, Single-Stranded / Q000737:chemistry*; D035683:MicroRNAs / Q000037:antagonists & inhibitors* / Q000737:chemistry; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D016376:Oligonucleotides, Antisense / Q000737:chemistry*
chemical_list: D015342:DNA Probes; D004277:DNA, Single-Stranded; D035683:MicroRNAs; D016376:Oligonucleotides, Antisense; D004247:DNA
keywords: Fluorescence polarization; LidNA; MicroRNA inhibitor; Surface plasmon resonance; ssDNA binding affinity