title: Crystal structure of the shrimp proliferating cell nuclear antigen: structural complementarity with WSSV DNA polymerase PIP-box.
abstract: DNA replication requires processivity factors that allow replicative DNA polymerases to extend long stretches of DNA. Some DNA viruses encode their own replicative DNA polymerase, such as the white spot syndrome virus (WSSV) that infects decapod crustaceans but still require host replication accessory factors. We have determined by X-ray diffraction the three-dimensional structure of the Pacific white leg shrimp Litopenaeus vannamei Proliferating Cell Nuclear Antigen (LvPCNA). This protein is a member of the sliding clamp family of proteins, that binds DNA replication and DNA repair proteins through a motif called PIP-box (PCNA-Interacting Protein). The crystal structure of LvPCNA was refined to a resolution of 3 Å, and allowed us to determine the trimeric protein assembly and details of the interactions between PCNA and the DNA. To address the possible interaction between LvPCNA and the viral DNA polymerase, we docked a theoretical model of a PIP-box peptide from the WSSV DNA polymerase within LvPCNA crystal structure. The theoretical model depicts a feasible model of interaction between both proteins. The crystal structure of shrimp PCNA allows us to further understand the mechanisms of DNA replication processivity factors in non-model systems.
authors.affiliation: Centro de Investigación en Alimentación y Desarrollo, A.C. Hermosillo, Sonora, México.
authors.forename: Jesus S
authors.initials: JS
authors.lastname: Carrasco-Miranda
authors.affiliation: Centro de Investigación en Alimentación y Desarrollo, A.C. Hermosillo, Sonora, México.
authors.forename: Alonso A
authors.initials: AA
authors.lastname: Lopez-Zavala
authors.affiliation: Departamento de Ciencias Químico Biológicas, Universidad de Sonora, Hermosillo, Sonora, México.
authors.forename: Aldo A
authors.initials: AA
authors.lastname: Arvizu-Flores
authors.affiliation: Centro de Investigación en Alimentación y Desarrollo, A.C. Hermosillo, Sonora, México.
authors.forename: Karina D
authors.initials: KD
authors.lastname: Garcia-Orozco
authors.affiliation: National Synchrotron Light Source, Brookhaven National Laboratory, Upton, New York, United States of America.
authors.forename: Vivian
authors.initials: V
authors.lastname: Stojanoff
authors.affiliation: Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología-Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México.
authors.forename: Enrique
authors.initials: E
authors.lastname: Rudiño-Piñera
authors.affiliation: Laboratorio Nacional de Genómica para la Biodiversidad, Centro de Investigación y Estudios Avanzados, Irapuato, Guanajuato, México.
authors.forename: Luis G
authors.initials: LG
authors.lastname: Brieba
authors.affiliation: Centro de Investigación en Alimentación y Desarrollo, A.C. Hermosillo, Sonora, México.
authors.forename: Rogerio R
authors.initials: RR
authors.lastname: Sotelo-Mundo
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018360:Crystallography, X-Ray; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry* / Q000378:metabolism; D062105:Molecular Docking Simulation; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000201:enzymology*; D010455:Peptides / Q000378:metabolism; D018809:Proliferating Cell Nuclear Antigen / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D016415:Sequence Alignment; D055672:Static Electricity; D040681:Structural Homology, Protein; D046848:White spot syndrome virus 1 / Q000201:enzymology*
chemical_list: D010455:Peptides; D018809:Proliferating Cell Nuclear Antigen; D004259:DNA-Directed DNA Polymerase
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