title: The V domain of dog PVRL4 (nectin-4) mediates canine distemper virus entry and virus cell-to-cell spread.
abstract: The entry of canine distemper virus (CDV) is a multistep process that involves the attachment of CDV hemagglutinin (H) to its cellular receptor, followed by fusion between virus and cell membranes. Our laboratory recently identified PVRL4 (nectin-4) to be the epithelial receptor for measles and canine distemper viruses. In this study, we demonstrate that the V domain of PVRL4 is critical for CDV entry and virus cell-to-cell spread. Furthermore, four key amino acid residues within the V domain of dog PVRL4 and two within the CDV hemagglutinin were shown to be essential for receptor-mediated virus entry.
authors.affiliation: The Department of Microbiology and Immunology, Dalhousie University, Halifax, Nova Scotia, Canada B3H 1X5; IWK Health Centre, Canadian Center for Vaccinology, Goldbloom Pavilion, Halifax, Nova Scotia, Canada B3H 1X5.
authors.forename: Sebastien
authors.initials: S
authors.lastname: Delpeut
authors.affiliation: The Department of Microbiology and Immunology, Dalhousie University, Halifax, Nova Scotia, Canada B3H 1X5; IWK Health Centre, Canadian Center for Vaccinology, Goldbloom Pavilion, Halifax, Nova Scotia, Canada B3H 1X5.
authors.forename: Ryan S
authors.initials: RS
authors.lastname: Noyce
authors.affiliation: The Department of Microbiology and Immunology, Dalhousie University, Halifax, Nova Scotia, Canada B3H 1X5; IWK Health Centre, Canadian Center for Vaccinology, Goldbloom Pavilion, Halifax, Nova Scotia, Canada B3H 1X5; The Department of Pediatrics, Dalhousie University, Halifax, Nova Scotia, Canada. Electronic address: chris.richardson@dal.ca.
authors.forename: Christopher D
authors.initials: CD
authors.lastname: Richardson
mesh_terms: D000818:Animals; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D004217:Distemper Virus, Canine / Q000502:physiology*; D004285:Dogs; D006389:Hemagglutinins, Viral / Q000378:metabolism; D054884:Host-Pathogen Interactions; D011991:Receptors, Virus / Q000378:metabolism*; D053586:Virus Internalization
chemical_list: D015815:Cell Adhesion Molecules; D006389:Hemagglutinins, Viral; D011991:Receptors, Virus
keywords: CDV; Canine distemper virus; Epithelial cell; Hemagglutinin; Nectin-4; PVRL4; Poliovirus receptor-like protein-4; Receptor; Syncytia formation; V domain; Virus entry