title: PAX6, a novel target of miR-335, inhibits cell proliferation and invasion in glioma cells.
abstract: Paired box 6 (PAX6), a highly conserved transcription factor, is important in glioma. However, the molecular mechanisms involved remain unclear. The present study demonstrated that the expression of PAX6 was significantly reduced with the malignancy of glioma and also identified PAX6 as a novel target of microRNA (miR)‑335, which was significantly upregulated in glioma. The inhibition of miR‑335 increased the protein expression of PAX6, whereas the upregulation of miR‑335 suppressed its expression in human glioma U251 and U87 cells. Furthermore, upregulation of miR-335 promoted U251 cell proliferation, colony formation and invasion, which was reversed by the overexpression of PAX6. Furthermore, the present study demonstrated that the effect of miR‑335 on U251 cell invasion was via the modulation of matrix metalloproteinase (MMP)‑2 and MMP‑9 expression by targeting PAX6. In conclusion, the present study demonstrated that PAX6, as a novel target of miR‑335, has an anti‑oncogenic function in glioma, and thus PAX6 may serve as a therapeutic target for glioma.
authors.affiliation: Department of Neurosurgery, Xiangya Hospital of Central South University, Changsha, Hunan 410008, P.R. China.
authors.forename: Quan
authors.initials: Q
authors.lastname: Cheng
authors.affiliation: Department of Neurosurgery, Brain Hospital of Hunan Province, The Affiliated Hospital of Hunan Traditional Chinese Medicine University, Changsha, Hunan 410007, P.R. China.
authors.forename: Hui
authors.initials: H
authors.lastname: Cao
authors.affiliation: Department of Neurosurgery, Xiangya Hospital of Central South University, Changsha, Hunan 410008, P.R. China.
authors.forename: Zigui
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Neurosurgery, Xiangya Hospital of Central South University, Changsha, Hunan 410008, P.R. China.
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Ma
authors.affiliation: Department of Neurosurgery, Xiangya Hospital of Central South University, Changsha, Hunan 410008, P.R. China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wan
authors.affiliation: Department of Neurosurgery, Xiangya Hospital of Central South University, Changsha, Hunan 410008, P.R. China.
authors.forename: Renjun
authors.initials: R
authors.lastname: Peng
authors.affiliation: Department of Neurosurgery, Xiangya Hospital of Central South University, Changsha, Hunan 410008, P.R. China.
authors.forename: Bing
authors.initials: B
authors.lastname: Jiang
mesh_terms: D020413:3' Untranslated Regions; D001483:Base Sequence; D001932:Brain Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D005136:Eye Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005910:Glioma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D018398:Homeodomain Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D035683:MicroRNAs / Q000378:metabolism*; D060787:Neoplasm Grading; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D012097:Repressor Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D015854:Up-Regulation
chemical_list: D020413:3' Untranslated Regions; D005136:Eye Proteins; D018398:Homeodomain Proteins; C556347:MIRN335 microRNA, human; D035683:MicroRNAs; D000071841:PAX6 Transcription Factor; C000605730:PAX6 protein, human; D051761:Paired Box Transcription Factors; D012333:RNA, Messenger; D012097:Repressor Proteins; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9