title: Modulating chemotaxis of lung cancer cells by using electric fields in a microfluidic device.
abstract: We employed direct-current electric fields (dcEFs) to modulate the chemotaxis of lung cancer cells in a microfluidic cell culture device that incorporates both stable concentration gradients and dcEFs. We found that the chemotaxis induced by a 0.5 μM/mm concentration gradient of epidermal growth factor can be nearly compensated by a 360 mV/mm dcEF. When the effect of chemical stimulation was balanced by the electrical drive, the cells migrated randomly, and the path lengths were largely reduced. We also demonstrated electrically modulated chemotaxis of two types of lung cancer cells with opposite directions of electrotaxis in this device.
authors.affiliation: Graduate Institute of Biomedical Electronics and Bioinformatics, National Taiwan University, Taipei 10617, Taiwan ; Research Center for Applied Sciences, Academia Sinica, 11529 Taipei, Taiwan.
authors.forename: Yu-Chiu
authors.initials: YC
authors.lastname: Kao
authors.affiliation: Institute of Biophotonics, National Yang-Ming University, 11221 Taipei, Taiwan ; Biophotonics & Molecular Imaging Research Center (BMIRC), National Yang-Ming University, 11221 Taipei, Taiwan.
authors.forename: Meng-Hua
authors.initials: MH
authors.lastname: Hsieh
authors.affiliation: Research Center for Applied Sciences, Academia Sinica, 11529 Taipei, Taiwan.
authors.forename: Chung-Chun
authors.initials: CC
authors.lastname: Liu
authors.affiliation: Research Center for Applied Sciences, Academia Sinica, 11529 Taipei, Taiwan.
authors.forename: Huei-Jyuan
authors.initials: HJ
authors.lastname: Pan
authors.affiliation: Department of Internal Medicine, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei 10002, Taiwan.
authors.forename: Wei-Yu
authors.initials: WY
authors.lastname: Liao
authors.affiliation: Research Center for Applied Sciences, Academia Sinica, 11529 Taipei, Taiwan ; Institute of Biophotonics, National Yang-Ming University, 11221 Taipei, Taiwan ; Biophotonics & Molecular Imaging Research Center (BMIRC), National Yang-Ming University, 11221 Taipei, Taiwan.
authors.forename: Ji-Yen
authors.initials: JY
authors.lastname: Cheng
authors.affiliation: Graduate Institute of Biomedical Electronics and Bioinformatics, National Taiwan University, Taipei 10617, Taiwan ; Department of Electrical Engineering, National Taiwan University, Taipei 10617, Taiwan.
authors.forename: Po-Ling
authors.initials: PL
authors.lastname: Kuo
authors.affiliation: Research Center for Applied Sciences, Academia Sinica, 11529 Taipei, Taiwan ; Institute of Biophotonics, National Yang-Ming University, 11221 Taipei, Taiwan ; Biophotonics & Molecular Imaging Research Center (BMIRC), National Yang-Ming University, 11221 Taipei, Taiwan.
authors.forename: Chau-Hwang
authors.initials: CH
authors.lastname: Lee
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