title: The role of Mss11 in Candida albicans biofilm formation.
abstract: Candida albicans is an opportunistic human pathogen that can form a biofilm on biotic or inert surfaces such as epithelia and clinical devices. In this study, we examine the formation of C. albicans biofilm by establishing a key gene-centered network based on protein-protein interaction (PPI) and gene expression datasets. Starting from C. albicans Cph1 and Efg1, transcription factors associated with morphogenesis of biofilm formation, a network elucidates the complex cellular process and predicts potential unknown components related to biofilm formation. Subsequently, we analyzed the functions of Mss11 among these identified proteins to test the efficiency of the proposed computational approach. MSS11-deleted mutants were compared with a wild-type strain, indicating that the mutant is defective in forming a mature biofilm and partially attenuates the virulence of C. albicans in an infected mouse model. Finally, a DNA microarray analysis was conducted to identify the potential target genes of C. albicans Mss11. The findings of this study clarify complex gene or protein interaction during the biofilm formation process of C. albicans, supporting the application of a systems biology approach to study fungal pathogenesis.
authors.affiliation: Institute of Molecular and Cellular Biology, National Tsing Hua University, No. 101, Section 2, Kuang-Fu Road, Hsinchu, 30013, Taiwan, ROC.
authors.forename: Pei-Wen
authors.initials: PW
authors.lastname: Tsai
authors.forename: Yu-Ting
authors.initials: YT
authors.lastname: Chen
authors.forename: Cheng-Yao
authors.initials: CY
authors.lastname: Yang
authors.forename: Hsueh-Fen
authors.initials: HF
authors.lastname: Chen
authors.forename: Te-Sheng
authors.initials: TS
authors.lastname: Tan
authors.forename: Tzung-Wei
authors.initials: TW
authors.lastname: Lin
authors.forename: Wen-Ping
authors.initials: WP
authors.lastname: Hsieh
authors.forename: Chung-Yu
authors.initials: CY
authors.lastname: Lan
mesh_terms: D000818:Animals; D018441:Biofilms / Q000254:growth & development*; D002176:Candida albicans / Q000472:pathogenicity / Q000502:physiology*; D002177:Candidiasis / Q000382:microbiology*; D005260:Female; D005656:Fungal Proteins / Q000502:physiology*; D015870:Gene Expression; D015966:Gene Expression Regulation, Fungal; D053263:Gene Regulatory Networks; D051379:Mice; D008807:Mice, Inbred BALB C; D014157:Transcription Factors / Q000502:physiology*; D014774:Virulence
chemical_list: D005656:Fungal Proteins; D014157:Transcription Factors
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