title: A perspective on optical developments in microfluidic platforms for Caenorhabditis elegans research.
abstract: Microfluidics offers unique ways of handling and manipulating microorganisms, which has particularly benefited Caenorhabditis elegans research. Optics plays a major role in these microfluidic platforms, not only as a read-out for the biological systems of interest but also as a vehicle for applying perturbations to biological systems. Here, we describe different areas of research in C. elegans developmental biology and behavior neuroscience enabled by microfluidics combined with the optical components. In particular, we highlight the diversity of optical tools and methods in use and the strategies implemented in microfluidics to make the devices compatible with optical techniques. We also offer some thoughts on future challenges in adapting advancements in optics to microfluidic platforms.
authors.affiliation: School of Chemical & Biomolecular Engineering, Georgia Institute of Technology, Atlanta, Georgia 30332, USA.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Aubry
authors.affiliation: School of Chemical & Biomolecular Engineering, Georgia Institute of Technology, Atlanta, Georgia 30332, USA.
authors.forename: Hang
authors.initials: H
authors.lastname: Lu
references.citation: Nature. 2006 May 4;441(7089):91-5
references.pmid: 16672971
references.citation: Nat Methods. 2011 Jan;8(1):35-8
references.pmid: 21191370
references.citation: Integr Biol (Camb). 2013 Feb;5(2):372-80
references.pmid: 23168494
references.citation: Annu Rev Neurosci. 2011;34:389-412
references.pmid: 21692661
references.citation: Nat Methods. 2006 Oct;3(10):793-5
references.pmid: 16896339
references.citation: Neuron. 2012 Mar 8;73(5):862-85
references.pmid: 22405199
references.citation: Proc Natl Acad Sci U S A. 2007 Aug 28;104(35):13891-5
references.pmid: 17715055
references.citation: Lab Chip. 2008 May;8(5):653-6
references.pmid: 18432331
references.citation: Nature. 2007 Nov 1;450(7166):63-70
references.pmid: 17972877
references.citation: Nat Methods. 2008 Oct;5(10):895-902
references.pmid: 18794862
references.citation: Opt Express. 2010 Dec 20;18(26):27499-510
references.pmid: 21197025
references.citation: Nat Neurosci. 2013 Jul;16(7):790-7
references.pmid: 23799471
references.citation: Nature. 2005 Nov 10;438(7065):179-84
references.pmid: 16281027
references.citation: Biomicrofluidics. 2009 Dec 23;3(4):44114
references.pmid: 20216976
references.citation: Nature. 2004 Jul 15;430(6997):317-22
references.pmid: 15220933
references.citation: Cell. 2010 Dec 23;143(7):1047-58
references.pmid: 21168201
references.citation: Anal Chim Acta. 2011 Sep 2;701(1):23-8
references.pmid: 21763804
references.citation: Chem Biol. 2008 May;15(5):427-37
references.pmid: 18482695
references.citation: Proc Natl Acad Sci U S A. 2008 Oct 21;105(42):16350-5
references.pmid: 18852466
references.citation: Lab Chip. 2010 Mar 7;10(5):589-97
references.pmid: 20162234
references.citation: Proc Natl Acad Sci U S A. 2010 Oct 26;107(43):18342-7
references.pmid: 20937901
references.citation: Nat Methods. 2012 Sep;9(9):889-95
references.pmid: 22936170
references.citation: Proc Natl Acad Sci U S A. 2012 Oct 2;109(40):16018-22
references.pmid: 22988076
references.citation: Nat Methods. 2011 Jun 12;8(7):599-605
references.pmid: 21666667
references.citation: Neuron. 2010 Dec 22;68(6):1173-86
references.pmid: 21172617
references.citation: J Neurosci Methods. 2010 Mar 30;187(2):229-34
references.pmid: 20096306
references.citation: Nat Methods. 2008 Jun;5(6):531-3
references.pmid: 18408725
references.citation: Neuron. 2008 Sep 25;59(6):959-71
references.pmid: 18817734
references.citation: Nat Rev Neurosci. 2012 Mar 20;13(4):251-66
references.pmid: 22430017
references.citation: Neuron. 2011 Jul 14;71(1):9-34
references.pmid: 21745635
references.citation: Biol Chem. 2012 Apr;393(4):235-48
references.pmid: 23029645
references.citation: Lab Chip. 2006 Oct;6(10):1274-6
references.pmid: 17102839
references.citation: Lab Chip. 2008 Sep;8(9):1432-5
references.pmid: 18818795
references.citation: Opt Lett. 1994 Jun 1;19(11):780-2
references.pmid: 19844443
references.citation: Lab Chip. 2010 Jul 21;10(14):1787-92
references.pmid: 20445943
references.citation: Nat Methods. 2007 Sep;4(9):727-31
references.pmid: 17704783
references.citation: Lab Chip. 2011 Nov 7;11(21):3698-702
references.pmid: 21935556
references.citation: J Neurosci Methods. 2008 Aug 15;173(1):20-6
references.pmid: 18579213
references.citation: Lab Chip. 2009 Jan 7;9(1):38-40
references.pmid: 19209332
references.citation: WormBook. 2013 Feb 22;:1-17
references.pmid: 23436808
references.citation: Nat Methods. 2012 Jun 28;9(7):656-7
references.pmid: 22743768
references.citation: J Neurochem. 2009 Jan;108(1):23-32
references.pmid: 19054282
references.citation: Nat Methods. 2013 Oct;10(10):1013-20
references.pmid: 24013820
references.citation: Proc Natl Acad Sci U S A. 2008 Aug 5;105(31):10670-5
references.pmid: 18663227
references.citation: Curr Opin Biotechnol. 2014 Feb;25:24-9
references.pmid: 24484877
references.citation: Lab Chip. 2010 Nov 7;10(21):2855-63
references.pmid: 20882233
references.citation: PLoS One. 2012;7(4):e35037
references.pmid: 22536350
references.citation: Lab Chip. 2007 Nov;7(11):1515-23
references.pmid: 17960280
references.citation: Nat Rev Genet. 2011 Nov 08;12(12):809-20
references.pmid: 22064512
references.citation: Lab Chip. 2009 Oct 7;9(19):2764-6
references.pmid: 19967110
references.citation: Methods Cell Biol. 2012;107:177-206
references.pmid: 22226524
references.citation: J Neurosci Methods. 2010 Aug 15;191(1):90-3
references.pmid: 20538016
references.citation: Br Med Bull. 1973 Sep;29(3):269-71
references.pmid: 4807330
references.citation: Nat Protoc. 2010 Dec;5(12):1888-902
references.pmid: 21127483
references.citation: Dev Growth Differ. 2013 May;55(4):491-507
references.pmid: 23635320
references.citation: Bioessays. 2012 Jun;34(6):458-65
references.pmid: 22396302
references.citation: Lab Chip. 2011 Nov 7;11(21):3689-3697
references.pmid: 21935539
references.citation: Philos Trans R Soc Lond B Biol Sci. 1986 Nov 12;314(1165):1-340
references.pmid: 22462104
references.citation: Genetics. 1974 May;77(1):71-94
references.pmid: 4366476
references.citation: Lab Chip. 2013 Aug 7;13(15):2963-71
references.pmid: 23708469
references.citation: Lab Chip. 2010 Jul 21;10(14):1862-8
references.pmid: 20461264
references.citation: Lab Chip. 2010 Oct 21;10(20):2758-63
references.pmid: 20820480
references.citation: Nat Methods. 2011 Sep 29;8(10):811-9
references.pmid: 21959136
references.citation: Lab Chip. 2012 Oct 7;12(19):3543-51
references.pmid: 22810383
references.citation: Nat Methods. 2008 Jul;5(7):637-43
references.pmid: 18568029
references.citation: Nat Methods. 2010 Aug;7(8):637-42
references.pmid: 20601950
references.citation: Lab Chip. 2009 Jan 7;9(1):151-7
references.pmid: 19209348
references.citation: Lab Chip. 2010 Jun 21;10(12):1509-17
references.pmid: 20383347
references.citation: Science. 2006 Sep 15;313(5793):1642-5
references.pmid: 16902090
references.citation: Nat Methods. 2012 Oct;9(10):977-80
references.pmid: 22902935
references.citation: PLoS One. 2011;6(9):e24666
references.pmid: 21969859
references.citation: Biomicrofluidics. 2010 Jul 19;4(3):
references.pmid: 20714369
references.citation: Nat Methods. 2012 Dec;9(12):1202-5
references.pmid: 23142873
references.citation: Nat Methods. 2013 Jul;10(7):665-70
references.pmid: 23666410
references.citation: Proc Natl Acad Sci U S A. 2012 Oct 16;109(42):17081-6
references.pmid: 23019581
references.citation: Cold Spring Harb Protoc. 2012 Jun 01;2012(6):647-56
references.pmid: 22661439
references.citation: Curr Biol. 2005 Dec 20;15(24):2279-84
references.pmid: 16360690
references.citation: Neuron. 2012 Nov 21;76(4):750-61
references.pmid: 23177960
references.citation: PLoS Biol. 2012;10(5):e1001331
references.pmid: 22629231