title: Study of virulence factors of uropathogenic Escherichia coli and its antibiotic susceptibility pattern.
abstract: BACKGROUND
  Urinary tract infection (UTI) is one of the most common nosocomial infections, caused by Escherichia coli. This study determined the presence of virulence factors in the organism and correlates it with the multi-drug resistance (MDR).
  
  
  OBJECTIVE
  The aim of the following study is to assess the virulence factors of uropathogenic E. coli and antibiotic susceptibility pattern.
  
  
  METHODS
  This was a prospective study conducted in the Department of Microbiology in PT. B. D. Sharma, PGIMS, Rohtak.
  
  
  METHODS
  The study was conducted over a period of 1 year. Urine samples received were processed as per standard microbiological procedures. Virulence factors such as hemolysin, hemagglutination, cell surface hydrophobicity, serum resistance, gelatinase and siderophore production were studied. The antimicrobial susceptibility was done as per Clinical and Laboratory Standard Institute Guidelines.
  
  
  METHODS
  The data was analyzed by using SPSS(Statistical Package for the social sciences) IBM Corporation version 17.0. A two sided P ≤ 0.05 was considered to be significant.
  
  
  RESULTS
  Hemolysin production was seen in 47.4%, hemagglutination in 74.8%, cell surface hydrophobicity in 61%, serum resistance in 59%, gelatinase in 67.5% and siderophore production in 88% isolates. Nitrofurantoin was found to be most effective followed by, gatifloxacin and gentamicin. Twenty nine percent (29.62%) isolates were MDR.
  
  
  CONCLUSIONS
  Therefore, the knowledge of virulence factors of E. coli and their antibiotic susceptibility pattern will help in better understanding of the organism and in the treatment of UTI.
authors.affiliation: Department of Microbiology, Pt. B.D. Sharm PGIMS, Rohtak, Haryana, India.
authors.forename: Seema
authors.initials: S
authors.lastname: Mittal
authors.forename: Madhu
authors.initials: M
authors.lastname: Sharma
authors.forename: Uma
authors.initials: U
authors.lastname: Chaudhary
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002648:Child; D002675:Child, Preschool; D003428:Cross Infection / Q000382:microbiology; D024901:Drug Resistance, Multiple, Bacterial; D004927:Escherichia coli Infections / Q000382:microbiology; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D011446:Prospective Studies; D014552:Urinary Tract Infections / Q000382:microbiology; D056689:Uropathogenic Escherichia coli / Q000187:drug effects* / Q000302:isolation & purification / Q000472:pathogenicity*; D037521:Virulence Factors / Q000032:analysis*; D055815:Young Adult
chemical_list: D000900:Anti-Bacterial Agents; D037521:Virulence Factors