title: [1,25-dihydroxyvitamin D3 pretreatment inhibits house dust mite-induced thymic stromal lymphopoietin release by human airway epithelial cells].
abstract: OBJECTIVE
  To investigate the effect of 1,25-dihydroxyvitamin D3 (1,25VD3) on house dust mites (HDM)-induced expression of thymic stromal lymphopoietin (TSLP) in human airway epithelial cells in vitro.
  
  
  METHODS
  Human airway epithelial 16HBE cells were incubated with 200, 400, and 800 U/L in the absence or presence of 1,25VD3 (10(-8) mol/L) for 6 h and 24 h, and TSLP mRNA and protein expressions in the cells were assessed using quantitative PCR and ELISA.
  
  
  RESULTS
  16HBE cells incubated with HDM at 200, 400, and 800 U/L showed significantly increased TSLP mRNA and protein expressions (P<0.05). Pretreatment of the cells with 1,25VD3 obviously lowered 400 U/L HDM-induced TSLP expressions (P<0.05), but 1,25VD3 added along with HDM in the cells did not produce significant effects on TSLP expressions (P=0.58).
  
  
  CONCLUSIONS
  Both 1,25VD3 and HDM can induce TSLP expression and release in 16HBE cells, but pretreatment with 1,25VD3 can decrease HDM-augmented TSLP expression in the cells.
authors.affiliation: Chronic Airway Disease Laboratory, Department of Respiratory and Critical Care Medicine, Southern Medical University, Guangzhou 510515, China.E-mail: abu861015@ foxmail.com.
authors.forename: Liqin
authors.initials: L
authors.lastname: Zhou
authors.forename: Hangming
authors.initials: H
authors.lastname: Dong
authors.forename: Haijin
authors.initials: H
authors.lastname: Zhao
authors.forename: Mengchen
authors.initials: M
authors.lastname: Zou
authors.forename: Lihong
authors.initials: L
authors.lastname: Yao
authors.forename: Fei
authors.initials: F
authors.lastname: Zou
authors.forename: Shaoxi
authors.initials: S
authors.lastname: Cai
mesh_terms: D000818:Animals; D001980:Bronchi / Q000166:cytology; D002117:Calcitriol / Q000494:pharmacology*; D002460:Cell Line; D016207:Cytokines / Q000378:metabolism*; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D039981:Pyroglyphidae; D000094632:Thymic Stromal Lymphopoietin
chemical_list: D016207:Cytokines; D002117:Calcitriol; D000094632:Thymic Stromal Lymphopoietin