title: A single nucleotide polymorphism in the translation elongation factor 1α gene correlates with the ability to produce fumonisin in Japanese Fusarium fujikuroi.
abstract: PCR-RFLP based on the translation elongation factor 1α (TEF) gene was developed to identify Fusarium fujikuroi in the Fusarium (Gibberella) fujikuroi species complex. Ninety-three strains, most of which were obtained from various sources in Japan, were identified as F. fujikuroi and their capability to produce fumonisin was investigated using an in vitro assay. Fumonisin production was detected in 50 strains isolated from maize, strawberry, wheat, and rice, whereas it was undetectable in 43 strains derived from rice seeds and rice seedlings carrying the bakanae disease, and from unknown sources. A single nucleotide polymorphism in the TEF gene (T618G) correlated with the ability to synthesize fumonisin.
authors.affiliation: Life Science Research Center, Gifu University, Gifu 501-1193, Japan. Electronic address: suga@gifu-u.ac.jp.
authors.forename: Haruhisa
authors.initials: H
authors.lastname: Suga
authors.affiliation: Faculty of Applied Biological Science, Gifu University, Gifu 501-1193, Japan.
authors.forename: Miha
authors.initials: M
authors.lastname: Kitajima
authors.affiliation: Faculty of Applied Biological Science, Gifu University, Gifu 501-1193, Japan.
authors.forename: Riku
authors.initials: R
authors.lastname: Nagumo
authors.affiliation: National Institute of Livestock and Grassland Science, National Agriculture and Food Research Organization (NARO), Tsukuba 305-0901, Japan.
authors.forename: Takao
authors.initials: T
authors.lastname: Tsukiboshi
authors.affiliation: National Institute of Livestock and Grassland Science, National Agriculture and Food Research Organization (NARO), Tsukuba 305-0901, Japan.
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Uegaki
authors.affiliation: National Agricultural Research Center for Kyushu Okinawa Region, NARO, Kumamoto 861-1192, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Nakajima
authors.affiliation: National Food Research Institute, NARO, Tsukuba 305-8642, Japan.
authors.forename: Masayo
authors.initials: M
authors.lastname: Kushiro
authors.affiliation: National Food Research Institute, NARO, Tsukuba 305-8642, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Nakagawa
authors.affiliation: Faculty of Applied Biological Science, Gifu University, Gifu 501-1193, Japan.
authors.forename: Masafumi
authors.initials: M
authors.lastname: Shimizu
authors.affiliation: River Basin Research Center, Gifu University, 501-1193 Gifu, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Kageyama
authors.affiliation: Faculty of Applied Biological Science, Gifu University, Gifu 501-1193, Japan.
authors.forename: Mitsuro
authors.initials: M
authors.lastname: Hyakumachi
mesh_terms: D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D037341:Fumonisins / Q000378:metabolism*; D005670:Fusarium / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D020648:Peptide Elongation Factor 1 / Q000235:genetics*; D010944:Plants / Q000382:microbiology; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide
chemical_list: D004271:DNA, Fungal; D037341:Fumonisins; D020648:Peptide Elongation Factor 1
keywords: Bakanae disease; Fungi; Gibberella fujikuroi; Mycotoxin; Rice pathogen