title: Induction of neutralizing antibodies to influenza A virus H7N9 by inactivated whole virus in mice and nonhuman primates.
abstract: We evaluated the immunogenicity of hemagglutinin (HA) in the context of inactivated H7N9/AH/1/13-PR8 whole-virion. At 4weeks after immunization with 15μg HA, mice produced hemagglutination inhibition (HI) titers of 1:192 and neutralizing antibodies of 1:317. Aluminum hydroxide (alum), or a booster immunization, or both increased HI to 1:768, 1:384, 1:896 and neutralizing antibodies to 1:1868, 1:2302, 1:10,000, respectively. Macaques generated HI of 1:190 or 1:360 and virus neutralizing titers of 1:280 or 1:658 at 3weeks after immunization with HA alone or with alum. Sera from immunized mice and macaques protected mice from infection of A/Anhui/1/2013 (H7N9), suggesting an H7N9 vaccine is immunologically feasible.
authors.affiliation: State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
authors.forename: Weiqi
authors.initials: W
authors.lastname: Pan
authors.affiliation: Anhui University, Anhui, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Han
authors.affiliation: Guangzhou Institutes of Biomedicine and Health (GIBH), Chinese Academy of Sciences, Guangzhou, China.
authors.forename: Zhenyuan
authors.initials: Z
authors.lastname: Dong
authors.affiliation: State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Niu
authors.affiliation: Guangzhou Institutes of Biomedicine and Health (GIBH), Chinese Academy of Sciences, Guangzhou, China.
authors.forename: Zhengfeng
authors.initials: Z
authors.lastname: Li
authors.affiliation: Institute of Laboratory Sciences (ILS), Chinese Academy of Medical Sciences, Beijing, China.
authors.forename: Linlin
authors.initials: L
authors.lastname: Bao
authors.affiliation: State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
authors.forename: Chufang
authors.initials: C
authors.lastname: Li
authors.affiliation: State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
authors.forename: Qinfang
authors.initials: Q
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
authors.forename: Zifeng
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Institute of Laboratory Sciences (ILS), Chinese Academy of Medical Sciences, Beijing, China.
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Li
authors.affiliation: Health Quarantine Laboratory, Guangdong Inspection and Quarantine Technology Center, Guangzhou, China.
authors.forename: Jicheng
authors.initials: J
authors.lastname: Huang
authors.affiliation: Guangzhou Institutes of Biomedicine and Health (GIBH), Chinese Academy of Sciences, Guangzhou, China.
authors.forename: Liqiang
authors.initials: L
authors.lastname: Feng
authors.affiliation: Institute of Laboratory Sciences (ILS), Chinese Academy of Medical Sciences, Beijing, China.
authors.forename: Chuan
authors.initials: C
authors.lastname: Qin
authors.affiliation: State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
authors.forename: Nanshan
authors.initials: N
authors.lastname: Zhong
authors.affiliation: State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China; Guangzhou Institutes of Biomedicine and Health (GIBH), Chinese Academy of Sciences, Guangzhou, China. Electronic address: chen002@gmail.com.
authors.forename: Ling
authors.initials: L
authors.lastname: Chen
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000534:Alum Compounds / Q000008:administration & dosage; D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood* / Q000627:therapeutic use; D000914:Antibodies, Viral / Q000097:blood* / Q000627:therapeutic use; D004195:Disease Models, Animal; D005260:Female; D006385:Hemagglutination Inhibition Tests; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000276:immunology; D007114:Immunization / Q000379:methods; D007116:Immunization, Passive; D064766:Influenza A Virus, H7N9 Subtype / Q000276:immunology*; D007252:Influenza Vaccines / Q000008:administration & dosage / Q000276:immunology; D008251:Macaca; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D009976:Orthomyxoviridae Infections / Q000276:immunology / Q000517:prevention & control
chemical_list: D000276:Adjuvants, Immunologic; D000534:Alum Compounds; D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D007252:Influenza Vaccines; C041524:aluminum sulfate
keywords: Antibody; H7N9; Hemagglutinin; Influenza; Neutralization