title: Phenotypic and in vivo functional characterization of immortalized human fetal liver cells.
abstract: We report the establishment and characterization of immortalized human fetal liver progenitor cells by expression of the Simian virus 40 large T (SV40 LT) antigen. Well-characterized cells at various passages were transplanted into nude mice with acute liver injury and tested for functional capacity. The SV40LT antigen-immortalized fetal liver cells showed a morphology similar to primary cells. Cultured cells demonstrated stable phenotypic expression in various passages, of hepatic markers such as albumin, CK 8, CK18, transcription factors HNF-4α and HNF-1α and CYP3A/7. The cells did not stain for any of the tested cancer-associated markers. Albumin, HNF-4α and CYP3A7 expression was confirmed by reverse transcription polymerase chain reaction (RT-PCR). Flow cytometry showed expression of some progenitor cell markers. In vivo study showed that the cells expressed both fetal and differentiated hepatocytes markers. Our study suggests new approaches to expand hepatic progenitor cells, analyze their fate in animal models aiming at cell therapy of hepatic diseases.
authors.affiliation: Laboratory of Transplantation and Regenerative Medicine, Sahlgrenska Academy at University of Gothenburg , Gothenburg , Sweden.
authors.forename: Pradeep B
authors.initials: PB
authors.lastname: Patil
authors.forename: Setara
authors.initials: S
authors.lastname: Begum
authors.forename: Meghnad
authors.initials: M
authors.lastname: Joshi
authors.forename: Marika I
authors.initials: MI
authors.lastname: Kleman
authors.forename: Michael
authors.initials: M
authors.lastname: Olausson
authors.forename: Suchitra
authors.initials: S
authors.lastname: Sumitran-Holgersson
mesh_terms: D000418:Albumins / Q000032:analysis / Q000235:genetics; D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D000951:Antigens, Neoplasm / Q000032:analysis; D000952:Antigens, Polyomavirus Transforming / Q000032:analysis* / Q000235:genetics; D001189:Aryl Hydrocarbon Hydroxylases / Q000032:analysis / Q000235:genetics; D014408:Biomarkers, Tumor / Q000032:analysis; D015815:Cell Adhesion Molecules / Q000032:analysis; D002454:Cell Differentiation; D002460:Cell Line; D051544:Cytochrome P-450 CYP3A / Q000032:analysis / Q000235:genetics; D000071858:Epithelial Cell Adhesion Molecule; D053686:Fetal Stem Cells / Q000737:chemistry / Q000166:cytology* / Q000637:transplantation; D015870:Gene Expression; D051538:Hepatocyte Nuclear Factor 1-alpha / Q000032:analysis; D051557:Hepatocyte Nuclear Factor 4 / Q000032:analysis / Q000235:genetics; D022781:Hepatocytes / Q000737:chemistry / Q000166:cytology* / Q000637:transplantation; D006801:Humans; D007633:Keratins / Q000032:analysis; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010641:Phenotype; D010957:Plasmids; D012333:RNA, Messenger / Q000032:analysis; D013539:Simian virus 40; D014162:Transfection; D016159:Tumor Suppressor Protein p53 / Q000032:analysis
chemical_list: D000418:Albumins; D015703:Antigens, CD; D000951:Antigens, Neoplasm; D000952:Antigens, Polyomavirus Transforming; D014408:Biomarkers, Tumor; D015815:Cell Adhesion Molecules; D000071858:Epithelial Cell Adhesion Molecule; C496592:HNF4A protein, human; D051538:Hepatocyte Nuclear Factor 1-alpha; D051557:Hepatocyte Nuclear Factor 4; D012333:RNA, Messenger; D016159:Tumor Suppressor Protein p53; C115012:human epithelial antigen-125; D007633:Keratins; D001189:Aryl Hydrocarbon Hydroxylases; C103123:CYP3A7 protein, human; D051544:Cytochrome P-450 CYP3A; C510163:CYP3A4 protein, human
keywords: fetal liver cells; hepatocytes; immortalized; transplantation
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