title: Chemical and genetic similarity and diversity of Ligularia anoleuca and L. fischeri collected in the Hengduan Mountains of China.
abstract: The sesquiterpenoid composition in the root and the DNA sequences of evolutionarily neutral regions were studied in Ligularia anoleuca and Ligularia fischeri (Asteraceae) collected in the Sichuan Province of China. LC-MS analysis showed that L. anoleuca populations from different localities had different chemical compositions. However, the isolated compounds were similar to each other, indicating that the differences in chemical composition were not large. The DNA analysis suggested that the two species were indistinguishable. Seventeen furanoeremophilanes and an eremophilane acetal were isolated.
authors.affiliation: Department of Chemistry and Research Center for Smart Molecules, Rikkyo University, Nishi-Ikebukuro, Toshima-ku, Tokyo 171-8501, Japan.
authors.forename: Anna
authors.initials: A
authors.lastname: Shimizu
authors.affiliation: Department of Chemistry and Research Center for Smart Molecules, Rikkyo University, Nishi-Ikebukuro, Toshima-ku, Tokyo 171-8501, Japan.
authors.forename: Yurika
authors.initials: Y
authors.lastname: Suzuki
authors.affiliation: Department of Life Science and Research Center for Life Science, Rikkyo University, Nishi-Ikebukuro, Toshima-ku, Tokyo 171-8501, Japan. Electronic address: hanai@rikkyo.ne.jp.
authors.forename: Ryo
authors.initials: R
authors.lastname: Hanai
authors.affiliation: Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Yamashiro-cho, Tokushima 770-8514, Japan.
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Okamoto
authors.affiliation: Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Yamashiro-cho, Tokushima 770-8514, Japan. Electronic address: tori@ph.bunri-u.ac.jp.
authors.forename: Motoo
authors.initials: M
authors.lastname: Tori
authors.affiliation: Kunming Institute of Botany, Chinese Academy of Science, Kunming 650204, China. Electronic address: gongxun@mail.kib.ac.cn.
authors.forename: Xun
authors.initials: X
authors.lastname: Gong
authors.affiliation: Department of Chemistry and Research Center for Smart Molecules, Rikkyo University, Nishi-Ikebukuro, Toshima-ku, Tokyo 171-8501, Japan. Electronic address: chkkuroda@rikkyo.ac.jp.
authors.forename: Chiaki
authors.initials: C
authors.lastname: Kuroda
mesh_terms: D019659:Asteraceae / Q000737:chemistry* / Q000235:genetics*; D001483:Base Sequence; D002681:China; D018744:DNA, Plant / Q000737:chemistry* / Q000235:genetics*; D008968:Molecular Conformation; D018517:Plant Roots / Q000737:chemistry / Q000235:genetics; D017422:Sequence Analysis, DNA; D012717:Sesquiterpenes / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D018744:DNA, Plant; D012717:Sesquiterpenes
keywords: Asteraceae; Diversity; Furanoeremophilane; ITS; Ligularia anoleuca; Ligularia fischeri; Sesquiterpenoid; atpB-rbcL