title: A nondigestible saccharide, fructooligosaccharide, increases the promotive effect of a flavonoid, α-glucosyl-isoquercitrin, on glucagon-like peptide 1 (GLP-1) secretion in rat intestine and enteroendocrine cells.
abstract: This study conducted in vivo and in situ experiments with rats to investigate the glucagon-like peptide-1 (GLP-1) secretion in response to oral or ileal administration of α-glucosyl-isoquercitrin (20-40 mmol in 2 mL; Q3G), fructooligosaccharides (200 mmol in 2 mL; FOS) and Q3G + FOS. Direct effects on GLP-1-producing l-cells were also examined by an in vitro study using a murine enteroendocrine cell line, GLUTag. To evaluate the plasma GLP-1 level, blood samples from jugular cannula for in vivo and portal cannula for in situ experiments were obtained before and after administration of Q3G, FOS, or Q3G + FOS. We found tendencies for increases but transient stimulation of GLP-1 secretion by Q3G in in vivo and in situ experiments. Although FOS alone did not have any effects, Q3G + FOS enhanced and prolonged high plasma GLP-1 level in both experiments. In addition, application of Q3G on GLUTag cells stimulated GLP-1 secretion while FOS enhanced the effect of Q3G. Our results suggest that Q3G + FOS possess the potential for the management or prevention of Type 2 diabetes mellitus (T2DM) by enhancing and prolonging the GLP-1 secretion via direct stimulation of GLP-1 producing l-cell.
authors.affiliation: Laboratory of Nutritional Biochemistry, Division of Applied Bioscience, Research Faculty of Agriculture, Graduate School of Agriculture, Hokkaido University, Sapporo, Japan.
authors.forename: Panchita
authors.initials: P
authors.lastname: Phuwamongkolwiwat
authors.forename: Tohru
authors.initials: T
authors.lastname: Hira
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hara
mesh_terms: D000818:Animals; D002460:Cell Line; D003924:Diabetes Mellitus, Type 2 / Q000517:prevention & control; D019858:Enteroendocrine Cells / Q000187:drug effects* / Q000378:metabolism; D052216:Glucagon-Like Peptide 1 / Q000378:metabolism*; D007082:Ileum / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D009844:Oligosaccharides / Q000494:pharmacology*; D011794:Quercetin / Q000031:analogs & derivatives* / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
chemical_list: D009844:Oligosaccharides; C116580:fructooligosaccharide; C016527:isoquercitrin; D052216:Glucagon-Like Peptide 1; D011794:Quercetin
keywords: Fructooligosaccharides; Glucagon-like peptide-1; α-Glucosyl-isoquercitrin