title: Detection of bovine herpesvirus 2 and bovine herpesvirus 4 DNA in trigeminal ganglia of naturally infected cattle by polymerase chain reaction.
abstract: Establishment of latent infection within specific tissues in the host is a common biological feature of the herpesviruses. In the case of bovine herpesvirus 2 (BoHV-2), latency is established in neuronal tissues, while bovine herpesvirus 4 (BoHV-4) and ovine herpesvirus 2 (OvHV-2) latent virus targets on cells of the monocytic lineage. This study was conducted in quest of BoHV-2, BoHV-4 and OvHV-2 DNA in two hundred trigeminal ganglia (TG) specimens, derived from one hundred clinically healthy cattle, majority of them naturally infected with bovine herpesvirus 1 (BoHV-1) and bovine herpesvirus 5 (BoHV-5). Total DNA extracted from ganglia was analyzed by polymerase chain reaction (PCR) designed to amplify part of the genes coding for BoHV-2, and BoHV-4 glycoprotein B and, for OvHV-2, the gene coding for phosphoribosylformylglycinamidine synthase-like protein. BoHV-2 DNA was detected in TG samples of two (2%) and BoHV-4 DNA in nine (9%) of the animals, whereas OvHV-2 DNA could not be detected in any of the TG DNA. The two animals in which BoHV-2 DNA was identified were also co-infected with BoHV-1 and BoHV-5. Within the nine animals in which BoHV-4 DNA was detected, six were also co-infected with BoHV-1 and BoHV-5. This report provides for the first time evidence that viral DNA from BoHV-2 and BoHV-4 can be occasionally detected in TG of naturally infected cattle. Likewise, in this report we provided for the first time evidence that the co-infection of cattle with three distinct bovine herpesviruses might be a naturally occurring phenomenon.
authors.affiliation: Laboratory of Virology, Department of Microbiology, Immunology and Parasitology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul (UFRGS), Av. Sarmento Leite 500, Porto Alegre, CEP 90.050-170, Rio Grande do Sul (RS), Brazil. Electronic address: camposvet@gmail.com.
authors.forename: F S
authors.initials: FS
authors.lastname: Campos
authors.affiliation: Laboratory of Virology, Department of Microbiology, Immunology and Parasitology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul (UFRGS), Av. Sarmento Leite 500, Porto Alegre, CEP 90.050-170, Rio Grande do Sul (RS), Brazil.
authors.forename: A C
authors.initials: AC
authors.lastname: Franco
authors.affiliation: Laboratory of Virology, Department of Microbiology, Immunology and Parasitology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul (UFRGS), Av. Sarmento Leite 500, Porto Alegre, CEP 90.050-170, Rio Grande do Sul (RS), Brazil.
authors.forename: M T
authors.initials: MT
authors.lastname: Oliveira
authors.affiliation: Laboratory of Virology, Department of Microbiology, Immunology and Parasitology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul (UFRGS), Av. Sarmento Leite 500, Porto Alegre, CEP 90.050-170, Rio Grande do Sul (RS), Brazil.
authors.forename: R
authors.initials: R
authors.lastname: Firpo
authors.affiliation: Laboratory of Virology, Department of Microbiology, Immunology and Parasitology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul (UFRGS), Av. Sarmento Leite 500, Porto Alegre, CEP 90.050-170, Rio Grande do Sul (RS), Brazil.
authors.forename: G
authors.initials: G
authors.lastname: Strelczuk
authors.affiliation: Laboratory of Virology, Department of Microbiology, Immunology and Parasitology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul (UFRGS), Av. Sarmento Leite 500, Porto Alegre, CEP 90.050-170, Rio Grande do Sul (RS), Brazil.
authors.forename: F E
authors.initials: FE
authors.lastname: Fontoura
authors.affiliation: Laboratory of Virology, Department of Microbiology, Immunology and Parasitology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul (UFRGS), Av. Sarmento Leite 500, Porto Alegre, CEP 90.050-170, Rio Grande do Sul (RS), Brazil.
authors.forename: M I R
authors.initials: MI
authors.lastname: Kulmann
authors.affiliation: Instituto de Virología, Centro de Investigaciones en Ciencias Veterinarias y Agronómicas (CICVyA), Instituto de tecnología Agropecuaria (INTA), N. Repetto y Los Reseros S/N, CC25 (B1712WAA), Castelar, Buenos Aires, Argentina.
authors.forename: S
authors.initials: S
authors.lastname: Maidana
authors.affiliation: Instituto de Virología, Centro de Investigaciones en Ciencias Veterinarias y Agronómicas (CICVyA), Instituto de tecnología Agropecuaria (INTA), N. Repetto y Los Reseros S/N, CC25 (B1712WAA), Castelar, Buenos Aires, Argentina.
authors.forename: S A
authors.initials: SA
authors.lastname: Romera
authors.affiliation: Molecular Microbiology Laboratory, Institute of Health Sciences, Feevale University, Rodovia RS-239 2755, Novo Hamburgo, CEP 93.352-000, Rio Grande do Sul (RS), Brazil.
authors.forename: F R
authors.initials: FR
authors.lastname: Spilki
authors.affiliation: Embrapa CNPSA, BR 153, Km 110, Post Box 21, Concórdia, CEP 89.700-000, Santa Catarina (SC), Brazil.
authors.forename: A D
authors.initials: AD
authors.lastname: Silva
authors.affiliation: Laboratory of Virology, Department of Preventive Veterinary Medicine, Faculty of Veterinary Medicine, Federal University of Pelotas (UFPel), University Campus, Post Box 354, Pelotas, CEP 96.010-900, Rio Grande do Sul (RS), Brazil.
authors.forename: S O
authors.initials: SO
authors.lastname: Hübner
authors.affiliation: Laboratory of Virology, Department of Microbiology, Immunology and Parasitology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul (UFRGS), Av. Sarmento Leite 500, Porto Alegre, CEP 90.050-170, Rio Grande do Sul (RS), Brazil; Institute for Veterinary Research "Desidério Finamor" (IPVDF), Estrada do Conde 6000, Eldorado do Sul, CEP 92.990-000, Rio Grande do Sul (RS), Brazil.
authors.forename: P M
authors.initials: PM
authors.lastname: Roehe
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000821:virology; D002460:Cell Line; D060085:Coinfection; D004279:DNA, Viral / Q000235:genetics; D006566:Herpesviridae Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D007242:Herpesvirus 1, Bovine / Q000502:physiology; D001911:Herpesvirus 2, Bovine / Q000235:genetics; D026021:Herpesvirus 4, Bovine / Q000235:genetics; D025881:Herpesvirus 5, Bovine / Q000502:physiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary*; D012668:Trigeminal Ganglion / Q000821:virology*
chemical_list: D004279:DNA, Viral
keywords: Bovine herpesvirus; Internal control; Latency; Ovine herpesvirus; PCR