title: Inhibition on calcium oxalate crystallization and repair on injured renal epithelial cells of degraded soybean polysaccharide.
abstract: This paper investigated the inhibitory effect of degraded soybean polysaccharide (DPS) on the growth of calcium oxalate (CaOxa) crystals. The results were compared with that of soybean polysaccharide without degradation (SPS). The data showed that DPS exhibited a much higher efficiency to inhibit CaOxa growth and stabilize calcium oxalate dihydrate (COD) compared with SPS. As DPS concentration increased, the soluble Ca(2+) ions significantly increased, the aggregation degree of calcium oxalate monohydrate (COM) crystals decreased, the shape of COD crystals became round and blunt, and the Zeta potential on CaOxa crystal surface reduced. The above results were all conducive for the inhibition of CaOxa crystallization. In addition, DPS displayed a distinct repairing effect on oxidative injured renal epithelial cells in African green monkey (Vero), with enhanced cell viability and extracellular superoxide dismutase activity after repair. The morphologies of the repaired cells and their regulatory capability on CaOxa growth were between the control and injured cells. The results indicated that the risk of stone formation can be reduced by DPS, and that DPS may be a potential green drug to prevent the formation of CaOxa stones.
authors.affiliation: Institute of Biomineralization and Lithiasis Research, Jinan University, Guangzhou 510632, China.
authors.forename: Xiu-Qiong
authors.initials: XQ
authors.lastname: Yao
authors.affiliation: Institute of Biomineralization and Lithiasis Research, Jinan University, Guangzhou 510632, China.
authors.forename: Jian-Ming
authors.initials: JM
authors.lastname: Ouyang
authors.affiliation: Institute of Biomineralization and Lithiasis Research, Jinan University, Guangzhou 510632, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Peng
authors.affiliation: Institute of Biomineralization and Lithiasis Research, Jinan University, Guangzhou 510632, China.
authors.forename: Wen-Yu
authors.initials: WY
authors.lastname: Zhu
authors.affiliation: Department of Urology, Xiang Ya Hospital, Central Southern University, Changsha 410008, China.
authors.forename: He-Qun
authors.initials: HQ
authors.lastname: Chen
mesh_terms: D000818:Animals; D002129:Calcium Oxalate / Q000037:antagonists & inhibitors* / Q000737:chemistry*; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002522:Chlorocebus aethiops; D003460:Crystallization; D004847:Epithelial Cells / Q000187:drug effects*; D007668:Kidney / Q000166:cytology / Q000187:drug effects*; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D013025:Glycine max; D014709:Vero Cells
chemical_list: D011134:Polysaccharides; D002129:Calcium Oxalate