title: Changes in plant cell-wall structure of corn stover due to hot compressed water pretreatment and enhanced enzymatic hydrolysis.
abstract: Corn stover is a potential feedstock for biofuel production. This work investigated physical and chemical changes in plant cell-wall structure of corn stover due to hot compressed water (HCW) pretreatment at 170-190 °C in a tube reactor. Chemical composition analysis showed the soluble hemicellulose content increased with pretreatment temperature, whereas the hemicellulose content decreased from 29 to 7 % in pretreated solids. Scanning electron microscopy revealed the parenchyma-type second cell-wall structure of the plant was almost completely removed at 185 °C, and the sclerenchyma-type second cell wall was greatly damaged upon addition of 5 mmol/L ammonium sulfate during HCW pretreatment. These changes favored accessibility for enzymatic action. Enzyme saccharification of solids by optimized pretreatment with HCW at 185 °C resulted in an enzymatic hydrolysis yield of 87 %, an enhancement of 77 % compared to the yield from untreated corn stover.
authors.affiliation: National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, P.O. Box 353, Beijing, 100190, China, wzhou@mail.ipe.ac.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhou
authors.forename: Maohua
authors.initials: M
authors.lastname: Yang
authors.forename: Caixia
authors.initials: C
authors.lastname: Wang
authors.forename: Jianfei
authors.initials: J
authors.lastname: Liu
authors.forename: Jianmin
authors.initials: J
authors.lastname: Xing
mesh_terms: D000645:Ammonium Sulfate / Q000494:pharmacology; D019149:Bioreactors; D002473:Cell Wall / Q000737:chemistry* / Q000187:drug effects / Q000648:ultrastructure; D002480:Cellulase / Q000378:metabolism / Q000494:pharmacology*; D006358:Hot Temperature; D006868:Hydrolysis; D008855:Microscopy, Electron, Scanning; D018515:Plant Leaves / Q000648:ultrastructure; D011134:Polysaccharides / Q000032:analysis / Q000648:ultrastructure*; D014867:Water; D003313:Zea mays / Q000033:anatomy & histology* / Q000166:cytology / Q000378:metabolism; D001617:beta-Glucosidase / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D011134:Polysaccharides; D014867:Water; C007916:hemicellulose; D001617:beta-Glucosidase; D002480:Cellulase; D000645:Ammonium Sulfate
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