title: Optimization of dengue immunoassay by label-free interferometric optical detection method.
abstract: In this communication we report a direct immunoassay for detecting dengue virus by means of a label-free interferometric optical detection method. We also demonstrate how we can optimize this sensing response by adding a blocking step able to significantly enhance the optical sensing response. The blocking reagent used for this optimization is a dry milk diluted in phosphate buffered saline. The recognition curve of dengue virus over the proposed surface sensor demonstrates the capacity of this method to be applied in Point of Care technology.
authors.affiliation: Group of Micro-nano Photonics and Biophotonics, Laser Center, and Center for Biomedical Technology, Universidad Politécnica de Madrid, 28040 Madrid, Spain. mariafe.laguna@upm.es.
authors.forename: María F
authors.initials: MF
authors.lastname: Laguna
authors.affiliation: Group of Micro-nano Photonics and Biophotonics, Laser Center, and Center for Biomedical Technology, Universidad Politécnica de Madrid, 28040 Madrid, Spain. m.holgado@upm.es.
authors.forename: Miguel
authors.initials: M
authors.lastname: Holgado
authors.affiliation: Group of Micro-nano Photonics and Biophotonics, Laser Center, and Center for Biomedical Technology, Universidad Politécnica de Madrid, 28040 Madrid, Spain. fj.sanza@upm.es.
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Sanza
authors.affiliation: Group of Micro-nano Photonics and Biophotonics, Laser Center, and Center for Biomedical Technology, Universidad Politécnica de Madrid, 28040 Madrid, Spain. alavin@etsii.upm.es.
authors.forename: Alvaro
authors.initials: A
authors.lastname: Lavín
authors.affiliation: Group of Micro-nano Photonics and Biophotonics, Laser Center, and Center for Biomedical Technology, Universidad Politécnica de Madrid, 28040 Madrid, Spain. alavin@etsii.upm.es.
authors.forename: Ana
authors.initials: A
authors.lastname: López
authors.affiliation: Group of Micro-nano Photonics and Biophotonics, Laser Center, and Center for Biomedical Technology, Universidad Politécnica de Madrid, 28040 Madrid, Spain. Rafael.casquel@upm.es.
authors.forename: Rafael
authors.initials: R
authors.lastname: Casquel
mesh_terms: D015374:Biosensing Techniques; D003716:Dengue Virus / Q000276:immunology* / Q000302:isolation & purification*; D007118:Immunoassay / Q000379:methods*; D007368:Interferometry / Q000379:methods*; D055095:Optics and Photonics / Q000379:methods*; D013194:Staining and Labeling
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