title: Matrilin-2 is a widely distributed extracellular matrix protein and a potential biomarker in the early stage of osteoarthritis in articular cartilage.
abstract: In this study, we first generated and characterized a polyclonal antibody against unique domain of matrlin-2 and then used this specific antibody to assess the expression pattern of matrilin-2 by immunohistochemistry. We found that marilin-2 is widely distributed in the connective tissues of many mouse tissues including heart, colon, penis, esophagus, lung, kidney, tracheal cartilage, developmental bone, and adult bone. The expression level of matrilin-2 was remarkably increased in the tissues of osteoarthritis developmental articular cartilage, compared to normal healthy tissues. Furthermore, we determined matrilin-2 expression in specific epithelial cells in stomach and ductal epithelial cells of salivary gland. In other tissues, the positive signals were mainly located around cardiac muscle cells and Purkinje fibers in the heart; corpus spongiosum in the penis; submucosa in the colon and esophagus; extracellular matrix of cartilage in the tracheal cartilage; and, glomerulus, the basement membrane of distal convoluted tubule and renal matrix in kidney. These observations indicated that the distribution pattern of matrilin-2 is heterogeneous in each tissue. Matrilin-2 may play an important role in the communication of matrix to matrix and matrix to cells and will be used as a potential biomarker in the early stage of osteoarthritis of articular cartilage.
authors.affiliation: Department of pathology, Beijing Tongren Hospital, Capital Medical University, No. 1 Dongjiaominxiang Street, Beijing 100730, China ; Department of Pathology, Qinghai Provincial People's Hospital, 2 Gonghe Road, Xining, Qinghai 810007, China.
authors.forename: Shukun
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Pathology, The Affiliated Xiangya Hospital of Xiangya School Medical School, Central South University, Changsha 410013, China.
authors.forename: Jinwu
authors.initials: J
authors.lastname: Peng
authors.affiliation: Department of Pathology, Qinghai Provincial People's Hospital, 2 Gonghe Road, Xining, Qinghai 810007, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Department of Surgery, SUNY Upstate Medical University, 750 E. Adams Street, Syracuse, NY 13210, USA.
authors.forename: Sara
authors.initials: S
authors.lastname: Javidiparsijani
authors.affiliation: Department of Surgery, SUNY Upstate Medical University, 750 E. Adams Street, Syracuse, NY 13210, USA.
authors.forename: Guirong
authors.initials: G
authors.lastname: Wang
authors.affiliation: Department of Anesthesiology, Hunan Provincial Tumor Hospital, The Affiliated Tumor Hospital of Xiangya School Medical School of Central South University, 283 Tongzipo Road, Changsha, Hunan 410013, China.
authors.forename: Yichun
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of pathology, Beijing Tongren Hospital, Capital Medical University, No. 1 Dongjiaominxiang Street, Beijing 100730, China.
authors.forename: Honggang
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Anesthesiology, Hunan Provincial Tumor Hospital, The Affiliated Tumor Hospital of Xiangya School Medical School of Central South University, 283 Tongzipo Road, Changsha, Hunan 410013, China.
authors.forename: Jingshi
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Pathology, Qinghai Provincial People's Hospital, 2 Gonghe Road, Xining, Qinghai 810007, China ; Department of Anesthesiology, Hunan Provincial Tumor Hospital, The Affiliated Tumor Hospital of Xiangya School Medical School of Central South University, 283 Tongzipo Road, Changsha, Hunan 410013, China.
authors.forename: Junming
authors.initials: J
authors.lastname: Luo
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002358:Cartilage, Articular / Q000378:metabolism* / Q000473:pathology; D002450:Cell Communication; D005109:Extracellular Matrix / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D005786:Gene Expression Regulation; D064235:Matrilin Proteins / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D010003:Osteoarthritis / Q000235:genetics / Q000378:metabolism* / Q000473:pathology
chemical_list: D015415:Biomarkers; C577542:Matn2 protein, mouse; D064235:Matrilin Proteins
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