title: Synthesis and characteristics of chitin and chitosan with the (2-hydroxy-3-trimethylammonium)propyl functionality, and evaluation of their antioxidant activity in vitro.
abstract: Quaternary amino groups were introduced into chitin and chitosan to obtain O-(2-hydroxy-3-trimethylammonium)propyl chitin (OHT-chitin) and N-(2-hydroxy-3-trimethylammonium)propyl chitosan (NHT-chitosan). They were characterized by FTIR spectra, and GPC. The molecular weight Mw of OHT-chitin and NHT-chitosan were 8986 and 9723 with polydispersity of 1.01 and 1.0 2, respectively. Their antioxidant activities in vitro were further studied. It was found that β-carotene-linoleic acid values of OHT-chitin and NHT-chitosan at 0.8 mg/mL were up to 91% and 96%, while that of chitosan was 40%. Based on photobleaching of α,α-diphenyl-β-picrylhydrazyl (DPPH) at 326 nm, the DPPH inhibitory activity of OHT-chitin and NHT-chitosan was 30.9% and 31.9% at 5 mg/mL, whereas chitosan only gave 4.8%. It was also exhibited that OHT-chitin and NHT-chitosan had better antioxidant activity than chitosan according to the reducing power as well as H2O2 scavenging activity.
authors.affiliation: College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310035, PR China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Zhang
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Geng
authors.forename: Hengjun
authors.initials: H
authors.lastname: Jiang
authors.forename: Jianrong
authors.initials: J
authors.lastname: Li
authors.forename: Jianying
authors.initials: J
authors.lastname: Huang
mesh_terms: D000975:Antioxidants / Q000138:chemical synthesis / Q000737:chemistry*; D001713:Biphenyl Compounds / Q000737:chemistry; D002686:Chitin / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D048271:Chitosan / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000737:chemistry; D019787:Linoleic Acid / Q000737:chemistry; D016877:Oxidants / Q000737:chemistry; D010851:Picrates / Q000737:chemistry; D019207:beta Carotene / Q000737:chemistry
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; C572320:N-((2-hydroxy-3-trimethylammonium)propyl)chitosan chloride; C581905:O-((2-hydroxy-3-trimethylammonium))propylchitin; D016877:Oxidants; D010851:Picrates; D019207:beta Carotene; D002686:Chitin; D048271:Chitosan; D019787:Linoleic Acid; D006861:Hydrogen Peroxide; C004931:1,1-diphenyl-2-picrylhydrazyl