title: Lack of confirmation of anti-inward rectifying potassium channel 4.1 antibodies as reliable markers of multiple sclerosis.
abstract: BACKGROUND
  auto-antibodies against the potassium channel inward rectifying potassium channel 4.1 (Kir4.1) have previously been identified in 46% of patients with multiple sclerosis (MS).
  
  
  OBJECTIVE
  to confirm these findings.
  
  
  METHODS
  we evaluated the presence of anti-Kir4.1 antibodies by enzyme-linked immunosorbent assay (ELISA) and immunofluorescence in 268 MS patients, 46 patients with other neurological diseases (OND) and 45 healthy controls.
  
  
  RESULTS
  anti-Kir4.1 antibodies were found in 7.5% of MS patients, 4.3% of OND patients and 4.4% of healthy controls. Immunofluorescence analysis did not identify any specific staining.
  
  
  CONCLUSIONS
  we confirmed the presence of anti-Kir4.1 antibodies in MS patients, but at a much lower prevalence than previously reported.
authors.affiliation: Gui-de-Chauliac Hospital, France.
authors.forename: Elodie
authors.initials: E
authors.lastname: Nerrant
authors.affiliation: The Neuroscience Institute of Montpellier, Inserm UMR1051, Saint Eloi Hospital, France.
authors.forename: Céline
authors.initials: C
authors.lastname: Salsac
authors.affiliation: Gui-de-Chauliac Hospital, France.
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Charif
authors.affiliation: Gui-de-Chauliac Hospital, France.
authors.forename: Xavier
authors.initials: X
authors.lastname: Ayrignac
authors.affiliation: Gui-de-Chauliac Hospital, France.
authors.forename: Clarisse
authors.initials: C
authors.lastname: Carra-Dalliere
authors.affiliation: CHU Nimes, France.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Castelnovo
authors.affiliation: Gui-de-Chauliac Hospital, France.
authors.forename: Radjiv
authors.initials: R
authors.lastname: Goulabchand
authors.affiliation: Saint Eloi Hospital, France.
authors.forename: Julie
authors.initials: J
authors.lastname: Tisseyre
authors.affiliation: The Neuroscience Institute of Montpellier, Inserm UMR1051, Saint Eloi Hospital, France.
authors.forename: Cédric
authors.initials: C
authors.lastname: Raoul
authors.affiliation: Saint Eloi Hospital, France.
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Eliaou
authors.affiliation: Gui-de-Chauliac Hospital, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Labauge
authors.affiliation: The Neuroscience Institute of Montpellier, Inserm UMR1051, Saint Eloi Hospital, France/Saint Eloi Hospital, France t-vincent@chu-montpellier.fr.
authors.forename: Thierry
authors.initials: T
authors.lastname: Vincent
mesh_terms: D000328:Adult; D001323:Autoantibodies / Q000097:blood* / Q000276:immunology; D001324:Autoantigens / Q000276:immunology; D015415:Biomarkers / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D008297:Male; D009103:Multiple Sclerosis / Q000097:blood / Q000276:immunology*; D024661:Potassium Channels, Inwardly Rectifying / Q000276:immunology*; D000098854:Kcnj10 Channel
chemical_list: D001323:Autoantibodies; D001324:Autoantigens; D015415:Biomarkers; D000098854:Kcnj10 Channel; D024661:Potassium Channels, Inwardly Rectifying
keywords: ELISA; Kir4.1 antibody; Multiple sclerosis; biomarker; diagnosis