title: The IncRNAs PCGEM1 and PRNCR1 are not implicated in castration resistant prostate cancer.
abstract: Long noncoding RNAs (IncRNAs) are increasingly implicated in cancer biology, contributing to essential cancer cell functions such as proliferation, invasion, and metastasis. In prostate cancer, several lncRNAs have been nominated as critical actors in disease pathogenesis. Among these, expression of PCGEM1 and PRNCR1 has been identified as a possible component in disease progression through the coordination of androgen receptor (AR) signaling (Yang et al., Nature 2013, see ref. [1]). However, concerns regarding the robustness of these findings have been suggested. Here, we sought to evaluate whether PCGEM1 and PRNCR1 are associated with prostate cancer. Through a comprehensive analysis of RNA-sequencing data (RNA-seq), we find evidence that PCGEM1 but not PRNCR1 is associated with prostate cancer. We employ a large cohort of >230 high-risk prostate cancer patients with long-term outcomes data to show that, in contrast to prior reports, neither gene is associated with poor patient outcomes. We further observe no evidence that PCGEM1 nor PRNCR1 interact with AR, and neither gene is a component of AR signaling. Thus, we conclusively demonstrate that PCGEM1 and PRNCR1 are not prognostic lncRNAs in prostate cancer and we refute suggestions that these lncRNAs interact in AR signaling.
authors.affiliation: Michigan Center for Translational Pathology, University of Michigan, Ann Arbor, Michigan USA.
authors.forename: John R
authors.initials: JR
authors.lastname: Prensner
authors.forename: Anirban
authors.initials: A
authors.lastname: Sahu
authors.forename: Matthew K
authors.initials: MK
authors.lastname: Iyer
authors.forename: Rohit
authors.initials: R
authors.lastname: Malik
authors.forename: Benjamin
authors.initials: B
authors.lastname: Chandler
authors.forename: Irfan A
authors.initials: IA
authors.lastname: Asangani
authors.forename: Anton
authors.initials: A
authors.lastname: Poliakov
authors.forename: Ismael A
authors.initials: IA
authors.lastname: Vergara
authors.forename: Mohammed
authors.initials: M
authors.lastname: Alshalalfa
authors.forename: Robert B
authors.initials: RB
authors.lastname: Jenkins
authors.forename: Elai
authors.initials: E
authors.lastname: Davicioni
authors.forename: Felix Y
authors.initials: FY
authors.lastname: Feng
authors.forename: Arul M
authors.initials: AM
authors.lastname: Chinnaiyan
mesh_terms: D015972:Gene Expression Regulation, Neoplastic; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D008297:Male; D009363:Neoplasm Proteins / Q000235:genetics; D011379:Prognosis; D011467:Prostate / Q000378:metabolism; D064129:Prostatic Neoplasms, Castration-Resistant / Q000235:genetics* / Q000401:mortality; D062085:RNA, Long Noncoding / Q000235:genetics*; D011944:Receptors, Androgen / Q000235:genetics
chemical_list: C410798:AR protein, human; D009363:Neoplasm Proteins; C588212:PCAT-1 lncRNA, human; C416084:PCGEM1 non-coding RNA, human; D062085:RNA, Long Noncoding; D011944:Receptors, Androgen
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