Purification and characterization of structural and functional properties of two lectins from a marine sponge Spheciospongia vesparia.
The purification, structural and functional characterization of two different lectins (named Svl-1 and Svl-2) has been reported from the marine sponge Spheciospongia vesparia. Purification procedure includes ammonium sulfate precipitation, combined with chromatography including Octyl-Sepharose-(NH4)SO4 hydrophobic column and DEAE-Toyopearl anion-exchange column using a high performance liquid chromatography. The similarities in function, specificity for saccharides, molecular weight, amino acid content and the N-terminal sequence of two lectins suggest that these proteins are isolectins. Amino acid composition and fluorescence analyses reveal that they contain an intrachain disulfide bridge, which might contribute to their high thermal stability. Furthermore, the purified lectins exhibit antibacterial activity against the gram-negative bacteria Pseudomonas aeruginosa and E. coli, indicating that they may be involved in a recognition strategy and may play a role in the defense response function of the sponge. This is the first report on the isolation of lectins from the S. vesparia. The purified lectins represent a potential possible candidate for future application in the recognition or treatment of cancer cells.
Bertha
B
Fenton
Roberto
R
Arreguín Espinosa
Edgar
E
Vázquez Contreras
Barbarin
B
Arreguín Lozano
Norma
N
Sánchez Sánchez
Enrique
E
García Hernández
Edgar
E
Zenteno Galindo
D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D059001:Aquatic Organisms / Q000737:chemistry*; D001419:Bacteria / Q000187:drug effects; D006384:Hemagglutination / Q000187:drug effects; D006801:Humans; D037102:Lectins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D011161:Porifera / Q000737:chemistry*; D011817:Rabbits; D017421:Sequence Analysis; D017385:Sequence Homology
D000900:Anti-Bacterial Agents; D037102:Lectins