Effects of exercise on mitochondrial function, neuroplasticity and anxio-depressive behavior of mice.
The present study was aimed at analyzing the effects of physical exercise on mitochondrial physiology, anxio-depressive-like behaviors and neuroplasticity in mice. Adult C57BL/6J male mice were isolated in home cages equipped or not with free-running wheels. After 6weeks of exercise, mice were tested in various behavioral paradigms to evaluate anxiety- and depressive-like behaviors. The hippocampi were dissected for neurochemical assays, including mitochondrial activity, monoamines content and the expression of genes involved in energy metabolism and brain-derived neurotrophic factor (BDNF) regulation. Exercise decreased anxiety-like behaviors in the open field and elevated plus maze, and exerted antidepressant-like effects in the tail suspension test. Exercise stimulated brain mitochondrial activity and increased resistance against rotenone, an inhibitor of complex I activity. Furthermore, mRNA expression of Bdnf, Gdnf, Tfam (mitochondrial transcription factor A), and Ndufa6 (mitochondrial I subunit) genes, as well as the phosphorylation of cAMP response element-binding protein were increased after exercise. In summary, exercise appears to engage mitochondrial pathways and to potentiate neuroplasticity and might be associated to mood improvement.
Departamento de Bioquímica, Universidade Federal de Santa Catarina, UFSC, Centro de Ciências Biológicas, CCB, Florianópolis, SC 88049-900, Brazil; Departamento de Farmacologia, Universidade Federal de Santa Catarina, UFSC, Centro de Ciências Biológicas, CCB, Florianópolis, SC 88049-900, Brazil. Electronic address: aderbalaguiar@gmail.com.
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Aguiar
INSERM UMR 894, Centre de Psychiatrie et Neurosciences, CPN, Paris 75634, France; Université Pierre et Marie Curie, UPMC, Site Pitié-Salpêtrière, Paris 75634, France.
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Stragier
Departamento de Bioquímica, Universidade Federal de Santa Catarina, UFSC, Centro de Ciências Biológicas, CCB, Florianópolis, SC 88049-900, Brazil.
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da Luz Scheffer
Departamento de Bioquímica, Universidade Federal de Santa Catarina, UFSC, Centro de Ciências Biológicas, CCB, Florianópolis, SC 88049-900, Brazil.
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Remor
Departamento de Farmacologia, Universidade Federal de Santa Catarina, UFSC, Centro de Ciências Biológicas, CCB, Florianópolis, SC 88049-900, Brazil.
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Oliveira
Departamento de Farmacologia, Universidade Federal de Santa Catarina, UFSC, Centro de Ciências Biológicas, CCB, Florianópolis, SC 88049-900, Brazil.
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Prediger
Departamento de Bioquímica, Universidade Federal de Santa Catarina, UFSC, Centro de Ciências Biológicas, CCB, Florianópolis, SC 88049-900, Brazil.
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Latini
Université Pierre et Marie Curie, UPMC, Site Pitié-Salpêtrière, Paris 75634, France; INSERM UMR 975, Centre de Recherche de l'Institut du Cerveau et de la Moelle Epinière, CRICM, Paris 75634, France.
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Raisman-Vozari
INSERM UMR 894, Centre de Psychiatrie et Neurosciences, CPN, Paris 75634, France; Université Pierre et Marie Curie, UPMC, Site Pitié-Salpêtrière, Paris 75634, France.
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Mongeau
INSERM UMR 894, Centre de Psychiatrie et Neurosciences, CPN, Paris 75634, France; Université Pierre et Marie Curie, UPMC, Site Pitié-Salpêtrière, Paris 75634, France.
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Lanfumey
D000818:Animals; D001007:Anxiety / Q000503:physiopathology*; D015306:Biogenic Monoamines / Q000378:metabolism; D001921:Brain / Q000187:drug effects / Q000503:physiopathology*; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism; D003863:Depression / Q000503:physiopathology*; D042967:Electron Transport Complex I / Q000037:antagonists & inhibitors / Q000378:metabolism; D005106:Exploratory Behavior / Q000502:physiology; D015870:Gene Expression / Q000502:physiology; D006624:Hippocampus / Q000187:drug effects / Q000503:physiopathology; D006799:Housing, Animal; D008297:Male; D018782:Maze Learning / Q000502:physiology; D008810:Mice, Inbred C57BL; D008928:Mitochondria / Q000187:drug effects / Q000502:physiology*; D009043:Motor Activity / Q000502:physiology*; D009473:Neuronal Plasticity / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D012934:Social Isolation; D014836:Volition / Q000502:physiology
D015306:Biogenic Monoamines; D019208:Brain-Derived Neurotrophic Factor; D012333:RNA, Messenger; D042967:Electron Transport Complex I
depression; epigenetic; hippocampus; mitochondria; mood; neurotrophin