Composite Scaffolds Containing Silk Fibroin, Gelatin, and Hydroxyapatite for Bone Tissue Regeneration and 3D Cell Culturing.
Three-dimensional (3D) silk fibroin scaffolds were modified with one of the major bone tissue derivatives (nano-hydroxyapatite) and/or a collagen derivative (gelatin). Adhesion and proliferation of mouse embryonic fibroblasts (MEF) within the scaffold were increased after modification with either nano-hydroxyapatite or gelatin. However, a significant increase in MEF adhesion and proliferation was observed when both additives were introduced into the scaffold. Such modified composite scaffolds provide a new and better platform to study wound healing, bone and other tissue regeneration, as well as artificial organ bioengineering. This system can further be applied to establish experimental models to study cell-substrate interactions, cell migration and other complex processes, which may be difficult to address using the conventional two-dimensional culture systems.
Biological Faculty, Moscow State University, Leninskie Gory, 1-12, 119991, Moscow, Russia.
M M
MM
Moisenovich
Biological Faculty, Moscow State University, Leninskie Gory, 1-12, 119991, Moscow, Russia.
A Yu
AY
Arkhipova
Biological Faculty, Moscow State University, Leninskie Gory, 1-12, 119991, Moscow, Russia.
A A
AA
Orlova
Biological Faculty, Moscow State University, Leninskie Gory, 1-12, 119991, Moscow, Russia.
M S
MS
Drutskaya
Biological Faculty, Moscow State University, Leninskie Gory, 1-12, 119991, Moscow, Russia.
S V
SV
Volkova
Biological Faculty, Moscow State University, Leninskie Gory, 1-12, 119991, Moscow, Russia.
S E
SE
Zacharov
Shumakov Institute of Transplantology and Artificial Organs, Federal Agency for High-Tech Medical Services, Shchukinskaya Str., 1, 113182, Moscow, Russia.
I I
II
Agapov
Biological Faculty, Moscow State University, Leninskie Gory, 1-12, 119991, Moscow, Russia.
M P
MP
Kirpichnikov
adhesion; composite biodegradable scaffolds; gelatin; hydroxyapatite; proliferation; silk fibroin