An HPLC method for the quantitation of 3-pentylbenzo[c]thiophen-1(3H)-one in dog plasma and its application to a pharmacokinetic study.
A simple, sensitive and reproducible high-performance liquid chromatography (HPLC) assay method was developed for the estimation of 3-pentylbenzo[c]thiophen-1(3H)-one (S5 ), a potential anti-ischemic stroke agent, in dog plasma. The analytical procedure involves protein precipitation of S5 and nobiletin (internal standard) from dog plasma with acetonitrile. Chromatographic separation was achieved on Sapphire C18 analytical column with methanol-water (80:20, v/v) as mobile phase. The eluate was monitored using a UV detector set at 260 nm. The calibration curves were linear over the range of 0.2-20 µg/mL. Absolute recoveries of S5 were 79.2-86.1% from dog plasma. The intra- and inter-day relative standard deviation precisions were <7 and 5%, respectively. The method was successfully applied to the pharmacokinetic study of S5 in beagle dogs.
State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, 210009, Jiangsu, People's Republic of China; Department of Pharmacology, China Pharmaceutical University, Nanjing, 210009, Jiangsu, People's Republic of China.
Ting-Ting
TT
Li
Kai
K
Hua
Xiao
X
Sheng
Ling
L
Liu
Jia-Ni
JN
Tan
Lin-Na
LN
Wang
Bo
B
Wei
Hui
H
Ji
Yi-Hua
YH
Zhang
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004285:Dogs; D004355:Drug Stability; D005260:Female; D016014:Linear Models; D008297:Male; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013876:Thiophenes / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics*
C576402:3-pentylbenzo(c)thiophen-1(3H)-one; D013876:Thiophenes
3-pentylbenzo[c]thiophen-1(3H)-one; beagles; high-performance liquid chromatography; pharmacokinetics