High-quality NMR structure of human anti-apoptotic protein domain Mcl-1(171-327) for cancer drug design.
A high-quality NMR solution structure is presented for protein hMcl-1(171-327) which comprises residues 171-327 of the human anti-apoptotic protein Mcl-1 (hMcl-1). Since this construct contains the three Bcl-2 homology (BH) sequence motifs which participate in forming a binding site for inhibitors of hMcl-1, it is deemed to be crucial for structure-based design of novel anti-cancer drugs blocking the Mcl1 related anti-apoptotic pathway. While the coordinates of an NMR solution structure for a corresponding construct of the mouse homologue (mMcl-1) are publicly available, our structure is the first atomic resolution structure reported for the 'apo form' of the human protein. Comparison of the two structures reveals that hMcl-1(171-327) exhibits a somewhat wider ligand/inhibitor binding groove as well as a different charge distribution within the BH3 binding groove. These findings strongly suggest that the availability of the human structure is of critical importance to support future design of cancer drugs.
Department of Chemistry, State University of New York at Buffalo, Buffalo, New York, United States of America.
Gaohua
G
Liu
Molecular Structure, Amgen, Thousand Oaks, California, United States of America.
Leszek
L
Poppe
Protein Science, Amgen, Thousand Oaks, California, United States of America.
Ken
K
Aoki
Protein Science, Amgen, Thousand Oaks, California, United States of America.
Harvey
H
Yamane
Protein Science, Amgen, Thousand Oaks, California, United States of America.
Jeffrey
J
Lewis
Department of Chemistry, State University of New York at Buffalo, Buffalo, New York, United States of America.
Thomas
T
Szyperski
D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D001665:Binding Sites; D015195:Drug Design; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D051379:Mice; D008958:Models, Molecular; D064549:Myeloid Cell Leukemia Sequence 1 Protein / Q000037:antagonists & inhibitors / Q000737:chemistry*; D010446:Peptide Fragments / Q000037:antagonists & inhibitors / Q000737:chemistry*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D055672:Static Electricity
D000970:Antineoplastic Agents; C579095:MCL1 protein, human; D064549:Myeloid Cell Leukemia Sequence 1 Protein; D010446:Peptide Fragments