LIM mineralization protein-1 inhibits the malignant phenotypes of human osteosarcoma cells.
Osteosarcoma (OS), also known as osteogenic sarcoma, is the most common primary malignancy of bone tumor in children and adolescents. However, its underlying molecular pathogenesis is still only vaguely understood. Recently, LIM mineralization protein-1 (LMP-1) was reported to be an essential positive regulator of osteoblast differentiation. In the present study, we found that the expression of LMP-1 is downregulated in OS tissues compared with adjacent normal tissues. Moreover, we restored the expression of LMP-1 through a recombinant adenovirus. Overexpression of LMP-1 inhibited cell proliferation and invasion, arrested cell cycle progression, and induced apoptosis in vitro. Finally, ectopic LMP-1 expression suppressed the expression of Runx2 and BMP-2 in OS cells. These data demonstrate that LMP-1 is an essential tumor suppressor in the OS pathological process, which will provide a new opportunity for discovering and identifying novel effective treatment strategies.
Department of Orthopedics, the First Affiliated Hospital of Nanchang University, Nanchang 330006, China. huiwenliu520@126.com.
Huiwen
H
Liu
Department of Children Health and Care, Jiangxi Maternal and Child Health Hospital, Nanchang 330006, China. huanglusea@126.com.
Lu
L
Huang
Department of Orthopedics, the First Affiliated Hospital of Nanchang University, Nanchang 330006, China. zhangzhongzu@126.com.
Zhongzu
Z
Zhang
Department of Oncology, the First Affiliated Hospital of Nanchang University, Nanchang 330006, China. zmzhang2008@gmail.com.
Zhanming
Z
Zhang
Department of Orthopedics, the First Affiliated Hospital of Nanchang University, Nanchang 330006, China. yuzhimingdoctor@126.com.
Zhiming
Z
Yu
Department of Orthopedics, the First Affiliated Hospital of Nanchang University, Nanchang 330006, China. chenxiang.best@163.com.
Xiang
X
Chen
Department of Orthopedics, the First Affiliated Hospital of Nanchang University, Nanchang 330006, China. ZhuoChen01@126.com.
Zhuo
Z
Chen
Department of Orthopedics, the First Affiliated Hospital of Nanchang University, Nanchang 330006, China. zengyongping1991@163.com.
Yongping
Y
Zen
Department of Orthopedics, the First Affiliated Hospital of Nanchang University, Nanchang 330006, China. yangjxjc@126.com.
Dong
D
Yang
Department of Orthopedics, the First Affiliated Hospital of Nanchang University, Nanchang 330006, China. Hzhimin2002@aliyun.com.
Zhimin
Z
Han
Department of Orthopedics, the First Affiliated Hospital of Nanchang University, Nanchang 330006, China. shuyong57@163.com.
Yong
Y
Shu
Department of Orthopedics, the First Affiliated Hospital of Nanchang University, Nanchang 330006, China. daimin@medmail.com.cn.
Min
M
Dai
Department of Orthopedics, the First Affiliated Hospital of Nanchang University, Nanchang 330006, China. osteo.caokai@gmail.com.
Kai
K
Cao
D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism*; D000256:Adenoviridae / Q000235:genetics; D055396:Bone Morphogenetic Protein 2 / Q000378:metabolism; D001859:Bone Neoplasms / Q000378:metabolism / Q000473:pathology; D059447:Cell Cycle Checkpoints; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D050659:Core Binding Factor Alpha 1 Subunit / Q000378:metabolism; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000378:metabolism*; D005822:Genetic Vectors / Q000378:metabolism; D006801:Humans; D060588:LIM Domain Proteins / Q000235:genetics / Q000378:metabolism*; D012516:Osteosarcoma / Q000378:metabolism / Q000473:pathology; D010641:Phenotype; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
D048868:Adaptor Proteins, Signal Transducing; C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D050659:Core Binding Factor Alpha 1 Subunit; D003598:Cytoskeletal Proteins; D060588:LIM Domain Proteins; C484380:PDLIM7 protein, human; C493709:RUNX2 protein, human; D011994:Recombinant Proteins