RNA recognition by a human antibody against brain cytoplasmic 200 RNA.
Diverse functional RNAs participate in a wide range of cellular processes. The RNA structure is critical for function, either on its own or as a complex form with proteins and other ligands. Therefore, analysis of the RNA conformation in cells is essential for understanding their functional mechanisms. However, no appropriate methods have been established as yet. Here, we developed an efficient strategy for panning and affinity maturation of anti-RNA human monoclonal antibodies from a naïve antigen binding fragment (Fab) combinatorial phage library. Brain cytoplasmic 200 (BC200) RNA, which is also highly expressed in some tumors, was used as an RNA antigen. We identified MabBC200-A3 as the optimal binding antibody. Mutagenesis and SELEX experiments showed that the antibody recognized a domain of BC200 in a structure- and sequence-dependent manner. Various breast cancer cell lines were further examined for BC200 RNA expression using conventional hybridization and immunoanalysis with MabBC200-A3 to see whether the antibody specifically recognizes BC200 RNA among the total purified RNAs. The amounts of antibody-recognizable BC200 RNA were consistent with hybridization signals among the cell lines. Furthermore, the antibody was able to discriminate BC200 RNA from other RNAs, supporting the utility of this antibody as a specific RNA structure-recognizing probe. Intriguingly, however, when permeabilized cells were subjected to immunoanalysis instead of purified total RNA, the amount of antibody-recognizable RNA was not correlated with the cellular level of BC200 RNA, indicating that BC200 RNA exists as two distinct forms (antibody-recognizable and nonrecognizable) in breast cancer cells and that their distribution depends on the cell type. Our results clearly demonstrate that anti-RNA antibodies provide an effective novel tool for detecting and analyzing RNA conformation.
Department of Chemistry, KAIST, Daejeon 305-701, Korea.
Euihan
E
Jung
Department of Chemistry, KAIST, Daejeon 305-701, Korea.
Jungmin
J
Lee
Department of Systems Immunology, College of Biomedical Science, Kangwon National University, Chuncheon 200-701, Korea.
Hyo Jeong
HJ
Hong
Molecular Imaging and Therapy Branch, National Cancer Center, Goyang-si 410-769, Korea.
Insoo
I
Park
Department of Chemistry, KAIST, Daejeon 305-701, Korea.
Younghoon
Y
Lee
D000911:Antibodies, Monoclonal / Q000235:genetics* / Q000276:immunology*; D001483:Base Sequence; D001921:Brain / Q000276:immunology*; D045744:Cell Line, Tumor; D003593:Cytoplasm / Q000235:genetics* / Q000276:immunology*; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics / Q000276:immunology; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry* / Q000276:immunology*
D000911:Antibodies, Monoclonal; D012313:RNA
BC200 RNA; RNA recognition; breast cancer cells; human monoclonal antibody; noncoding RNA