Potent anti-inflammatory activity of carbohydrate polymer with oxide of zinc.
Pebisut is a biological adhesive composed of naturally occurring carbohydrates combined with zinc oxide (ZnO) initially used as a coadjutant for healing of anastomoses. Likewise some works demonstrated that carbohydrate complexes exerts anti-inflammatory activity and it is widely known that ZnO modulate inflammation. However, the direct effects of Pebisut on isolated cells and acute inflammatory responses remained to be investigated. The present study evaluated anti-inflammatory effect of Pebisut using lipopolysaccharide (LPS) stimulated human mononuclear cells, chemotaxis, and cell infiltration in vivo in a murine model of peritonitis. Our data show that human cells treated with different dilutions of Pebisut release less IL-6, IL-1 β , and IL-8 after LPS stimuli compared with the control treated cells. In addition, Pebisut lacked chemotactic activity in human mononuclear cells but was able to reduce chemotaxis towards CCL2, CCL5, and CXCL12 that are representative mononuclear cells chemoattractants. Finally, in a murine model of peritonitis, we found less number of macrophages (F4/80(+)) and T lymphocytes (CD3(+)) in peritoneal lavages from animals treated with Pebisut. Our results suggest that Pebisut has anti-inflammatory activity, which might have a beneficial effect during anastomoses healing or wounds associated with excessive inflammation.
Immunobiology Laboratory, Mexico's Juarez Hospital, Ministry of Health, 07760 Mexico City, Mexico.
Mario Adan
MA
Moreno-Eutimio
Immunobiology Laboratory, Mexico's Juarez Hospital, Ministry of Health, 07760 Mexico City, Mexico.
Nayeli Goreti
NG
Nieto-Velázquez
Immunobiology Laboratory, Mexico's Juarez Hospital, Ministry of Health, 07760 Mexico City, Mexico.
Lorena
L
Espinosa-Monroy
Biochemistry Department, National Institute of Perinatology, Ministry of Health, 11000 Mexico City, Mexico.
Yessica
Y
Torres-Ramos
Biochemistry Department, National Institute of Perinatology, Ministry of Health, 11000 Mexico City, Mexico.
Araceli
A
Montoya-Estrada
Health Sciences Faculty, Anahuac University, 52786 State of Mexico, Mexico.
Jorge
J
Cueto
Health Research Policies, Coordinating Commission of National Health Institutes and Highly Specialized Hospitals, 01900 Mexico City, Mexico.
Juan Jose
JJ
Hicks
Mexico's Juarez Hospital, Ministry of Health, 07760 Mexico City, Mexico.
Gustavo
G
Acosta-Altamirano
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D002241:Carbohydrates / Q000494:pharmacology*; D002633:Chemotaxis / Q000187:drug effects; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D008070:Lipopolysaccharides / Q000633:toxicity; D017737:Macrophages, Peritoneal / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D010538:Peritonitis / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D013601:T-Lymphocytes / Q000378:metabolism / Q000473:pathology; D015034:Zinc Oxide / Q000494:pharmacology*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D002241:Carbohydrates; D016207:Cytokines; D008070:Lipopolysaccharides; D015034:Zinc Oxide