Inhibition of Salmonella typhi growth using extremely low frequency electromagnetic (ELF-EM) waves at resonance frequency.
OBJECTIVE
Typhoid is a serious disease difficult to be treated with conventional drugs. The aim of this study was to demonstrate a new method for the control of Salmonella typhi growth, through the interference with the bioelectric signals generated from the microbe during cell division by extremely low frequency electromagnetic waves (ELF-EMW-ELF-EM) at resonance frequency.


RESULTS
Isolated Salmonella typhi was subjected to square amplitude modulated waves (QAMW) with different modulation frequencies from two generators with constant carrier frequency of 10 MHz, amplitude of 10 Vpp, modulating depth ± 2 Vpp and constant field strength of 200 V m(-1) at 37°C. Both the control and exposed samples were incubated at the same conditions during the experiment. The results showed that there was highly significant inhibition effect for Salm. typhi exposed to 0·8 Hz QAMW for a single exposure for 75 min. Dielectric relaxation, TEM and DNA results indicated highly significant changes in the molecular structure of the DNA and cellular membrane resulting from the exposure to the inhibiting EM waves.


CONCLUSIONS
It was concluded that finding out the inhibiting resonance frequency of ELF-EM waves that deteriorates Salm. typhi growth will be promising method for the treatment of Salm. typhi infection either in vivo or in vitro.


CONCLUSIONS
This new non-invasive technique for treatment of bacterial infections is of considerable interest for the use in medical and biotechnological applications.
Biophysics Department, Faculty of Science, Cairo University, Cairo, Egypt.
M A
MA
Fadel
S A
SA
Mohamed
A M
AM
Abdelbacki
A H
AH
El-Sharkawy
D004269:DNA, Bacterial / Q000528:radiation effects; D060733:Electromagnetic Radiation; D012485:Salmonella typhi / Q000254:growth & development / Q000528:radiation effects* / Q000648:ultrastructure
D004269:DNA, Bacterial
Salmonella typhi; bacterial infection; electromagnetic effect; growth inhibition; resonance frequency