Changes in mitochondrial membrane composition and oxidative status during rapid growth, maturation and aging in zebrafish, Danio rerio.
Considering membranes and membrane components as possible pacemakers of the main processes taking place inside mitochondria, changes in phospholipids or fatty acids could play a central role linking different mechanisms involved in cumulative damage to cell molecules and dysfunction during periods of high stress, such as rapid growth and aging. Changes affecting either lipid class or fatty acid compositions could affect phospholipid and membrane properties and alter mitochondrial function and cell viability. In the present study, mitochondrial oxidative status and mitochondrial membrane phospholipid compositions were analyzed throughout the life-cycle of zebrafish. TBARS content significantly increased in 18-month-old fish while aconitase activity decreased in 24-month-old fish, which have been related with oxidative damage to molecules. Mitochondria-specific superoxide dismutase decreased in 24-month-old animals although this change was not statistically significant. Age affected both mitochondrial phospholipid content and the peroxidation index of most phospholipid classes suggesting that oxidative damage to mitochondrial lipids was occurring.
Institute of Aquaculture, School of Natural Sciences, University of Stirling, Stirling FK9 4LA, Scotland, United Kingdom. Electronic address: pedro.almaida@stir.ac.uk.
Pedro F
PF
Almaida-Pagán
Department of Physiology, Faculty of Biology, University of Murcia, 30100 Murcia, Spain.
Alejandro
A
Lucas-Sánchez
Institute of Aquaculture, School of Natural Sciences, University of Stirling, Stirling FK9 4LA, Scotland, United Kingdom.
Douglas R
DR
Tocher
D000154:Aconitate Hydratase / Q000235:genetics / Q000378:metabolism; D000375:Aging / Q000378:metabolism*; D000818:Animals; D005227:Fatty Acids / Q000032:analysis / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D015227:Lipid Peroxidation; D008297:Male; D008928:Mitochondria / Q000737:chemistry / Q000378:metabolism*; D051336:Mitochondrial Membranes / Q000737:chemistry / Q000378:metabolism*; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D010743:Phospholipids / Q000032:analysis / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D015027:Zebrafish / Q000235:genetics / Q000378:metabolism*
D005227:Fatty Acids; D010743:Phospholipids; D017392:Thiobarbituric Acid Reactive Substances; D013482:Superoxide Dismutase; D000154:Aconitate Hydratase
Aging; Cardiolipin; Danio rerio; Fatty acid; Mitochondrion; Oxidative stress