Sex and exercise interact to alter the expression of anabolic androgenic steroid-induced anxiety-like behaviors in the mouse.
Anabolic androgenic steroids (AAS) are taken by both sexes to enhance athletic performance and body image, nearly always in conjunction with an exercise regime. Although taken to improve physical attributes, chronic AAS use can promote negative behavior, including anxiety. Few studies have directly compared the impact of AAS use in males versus females or assessed the interaction of exercise and AAS. We show that AAS increase anxiety-like behaviors in female but not male mice and that voluntary exercise accentuates these sex-specific differences. We also show that levels of the anxiogenic peptide corticotrophin releasing factor (CRF) are significantly greater in males, but that AAS selectively increase CRF levels in females, thus abrogating this sex-specific difference. Exercise did not ameliorate AAS-induced anxiety or alter CRF levels in females. Exercise was anxiolytic in males, but this behavioral outcome did not correlate with CRF levels. Brain-derived neurotrophic factor (BDNF) has also been implicated in the expression of anxiety. As with CRF, levels of hippocampal BDNF mRNA were significantly greater in males than females. AAS and exercise were without effect on BDNF mRNA in females. In males, anxiolytic effects of exercise correlated with increased BDNF mRNA, however AAS-induced changes in BDNF mRNA and anxiety did not. In sum, we find that AAS elicit sex-specific differences in anxiety and that voluntary exercise accentuates these differences. In addition, our data suggest that these behavioral outcomes may reflect convergent actions of AAS and exercise on a sexually differentiated CRF signaling system within the extended amygdala.
Department of Physiology & Neurobiology, Hinman Box 7701, The Geisel School of Medicine at Dartmouth, Hanover, NH 03755, USA.
Marie M
MM
Onakomaiya
Department of Physiology & Neurobiology, Hinman Box 7701, The Geisel School of Medicine at Dartmouth, Hanover, NH 03755, USA.
Donna M
DM
Porter
Department of Neurobiology, Northwestern University, 2205 Tech Drive, Hogan 2-160, Evanston, IL 60208, USA.
Joseph G
JG
Oberlander
Department of Physiology & Neurobiology, Hinman Box 7701, The Geisel School of Medicine at Dartmouth, Hanover, NH 03755, USA. Electronic address: leslie.henderson@dartmouth.edu.
Leslie P
LP
Henderson
D045930:Anabolic Agents / Q000633:toxicity*; D000728:Androgens / Q000633:toxicity*; D000818:Animals; D001007:Anxiety / Q000139:chemically induced* / Q000523:psychology*; D001835:Body Weight / Q000187:drug effects; D002755:Choice Behavior; D003346:Corticotropin-Releasing Hormone / Q000378:metabolism; D005260:Female; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010805:Physical Conditioning, Animal / Q000502:physiology*; D013216:Reflex, Startle / Q000187:drug effects; D012727:Sex Characteristics; D012726:Sexual Behavior, Animal / Q000502:physiology*; D012919:Social Behavior; D013256:Steroids / Q000633:toxicity*
D045930:Anabolic Agents; D000728:Androgens; D013256:Steroids; D003346:Corticotropin-Releasing Hormone
Acoustic startle response; Amygdala; Anabolic androgenic steroids; Anxiety; Bed nucleus of the stria terminalis; Brain derived neurotrophic factor (BDNF); Corticotrophin releasing factor (CRF or CRH); Exercise; Sex-specific; Social interaction