Generation of iPS Cells from Human Hair Follice Dermal Papilla Cells.
Dermal papilla (DP) cells are unique regional stem cells of the skin that induce formation of a hair follicle and its regeneration cycle. DP are multipotent stem cells; therefore we supposed that the efficiency of DPC reprogramming could exceed that of dermal fibroblasts reprogramming. We generated induced pluripotent stem cells from human DP cells using lentiviral transfection with Oct4, Sox2, Klf4, and c-Myc, and cultivation of cells both in a medium supplemented with valproic acid and at a physiological level of oxygen (5%). The efficiency of DP cells reprogramming was ~0.03%, while the efficiency of dermal fibroblast reprogramming under the same conditions was ~0.01%. Therefore, we demonstrated the suitability of DP cells as an alternative source of iPS cells.
Koltsov Institute of Developmental Biology, Russian Academy of Sciences, Vavilova str., 26, 117808, Moscow, Russia ; Pirogov Russian National Research Medical University, Ostrovitianov str., 1, 117997, Moscow, Russia.
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Muchkaeva
Koltsov Institute of Developmental Biology, Russian Academy of Sciences, Vavilova str., 26, 117808, Moscow, Russia ; Pirogov Russian National Research Medical University, Ostrovitianov str., 1, 117997, Moscow, Russia.
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Dashinimaev
Pirogov Russian National Research Medical University, Ostrovitianov str., 1, 117997, Moscow, Russia.
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Artyuhov
Koltsov Institute of Developmental Biology, Russian Academy of Sciences, Vavilova str., 26, 117808, Moscow, Russia ; Pirogov Russian National Research Medical University, Ostrovitianov str., 1, 117997, Moscow, Russia.
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Myagkova
Koltsov Institute of Developmental Biology, Russian Academy of Sciences, Vavilova str., 26, 117808, Moscow, Russia ; Pirogov Russian National Research Medical University, Ostrovitianov str., 1, 117997, Moscow, Russia.
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Vorotelyak
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vaviolva str., 32, 119991, Moscow, Russia.
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Yegorov
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vaviolva str., 32, 119991, Moscow, Russia.
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Vishnyakova
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vaviolva str., 32, 119991, Moscow, Russia ; Shemyakin and Ovchinnikov Instituse of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklay str., 16/10, 117997, Moscow, Russia.
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Kravchenko
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vaviolva str., 32, 119991, Moscow, Russia ; Shemyakin and Ovchinnikov Instituse of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklay str., 16/10, 117997, Moscow, Russia.
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Chumakov
Koltsov Institute of Developmental Biology, Russian Academy of Sciences, Vavilova str., 26, 117808, Moscow, Russia.
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Terskikh
Koltsov Institute of Developmental Biology, Russian Academy of Sciences, Vavilova str., 26, 117808, Moscow, Russia.
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Vasiliev
Hair follicle dermal papilla (DP) cells; induced pluripotent stem (iPS) cells; reprogramming