Synthesis of Gold Nanoparticles Coated with Aspartic Acid and Their Conjugation with FVIII Protein and FVIII Antibody.
Carboxylate-modified gold nanoparticles (GNPs) were synthesized in a simple one-step process based on the reduction of tetrachloroauric acid by aspartic acid in water. GNPs were identified by UV-Vis spectroscopy, dynamic light scattering (DLS) and transmission electron microscopy. Conjugation of protein molecules with functionalized nanoparticles was performed through electrostatic interaction. The GNP-protein conjugates were characterized by gel electrophoresis. The interaction between functionalized GNPs and protein molecules lead to conformational transition of protein structure after conjugation of protein with GNPs. This process was investigated by fluorescence spectroscopy and circular dichroism spectroscopy.
Pilot Biotechnology Department, Pasteur Institute of Iran, No. 358, 12 Farvardin Street, Jomhoori Avenue, 13169-43551 Tehran, Iran ; Zanjan University, Zanjan, Iran.
Maryam Farahnak
MF
Zarabi
Pilot Biotechnology Department, Pasteur Institute of Iran, No. 358, 12 Farvardin Street, Jomhoori Avenue, 13169-43551 Tehran, Iran.
Ali
A
Farhangi
Pilot Biotechnology Department, Pasteur Institute of Iran, No. 358, 12 Farvardin Street, Jomhoori Avenue, 13169-43551 Tehran, Iran.
Samaneh Khademi
SK
Mazdeh
Zanjan University, Zanjan, Iran.
Zahra
Z
Ansarian
Pilot Biotechnology Department, Pasteur Institute of Iran, No. 358, 12 Farvardin Street, Jomhoori Avenue, 13169-43551 Tehran, Iran.
Davod
D
Zare
Pilot Biotechnology Department, Pasteur Institute of Iran, No. 358, 12 Farvardin Street, Jomhoori Avenue, 13169-43551 Tehran, Iran.
Mohammad Reza
MR
Mehrabi
Pilot Biotechnology Department, Pasteur Institute of Iran, No. 358, 12 Farvardin Street, Jomhoori Avenue, 13169-43551 Tehran, Iran.
Azim
A
Akbarzadeh
Aspartic acid; Bioconjugation; Colloid; FVIII protein; Gold nanoparticles