Liver oxidation and inflammation in Fa/Fa rats fed glucomannan/spirulina-surimi.
The effect of high-fat squid-surimi diets enriched in glucomannan or glucomannan-spirulina on lipemia, liver glutathione status, antioxidant enzymes and inflammation biomarkers was determined in Zucker Fa/Fa rats. Groups of eight rats each received for 7weeks the squid-surimi control (C), glucomannan-enriched squid-surimi (G) and glucomannan-spirulina enriched squid-surimi (GS). Liver weight, cytochrome P450 7A1 expression and cholesterolemia were decreased in G and GS vs. C, improving glutathione red-ox index (p<0.05). G also showed increased glutathione reductase (GR) levels vs. C, but reduced the endothelial (eNOS) and increased the inducible nitric oxide synthase (iNOS) and tumor necrosis factor-alpha (TNF-α) levels (p<0.05). The GS diet improved superoxide dismutase, catalase, glutathione peroxidase and GR activities and eNOS, iNOS and TNF-α levels (p<0.05). The glucomannan enriched surimi-diet induced hypocholesterolemic, antioxidant and proinflammatory effects, while the addition of 3g/kg spirulina kept those hypocholesterolemic and antioxidant effects but reduced the inflammation observed.
Departamento de Nutrición y Bromatología I (Nutrición), Facultad de Farmacia, Universidad Complutense de Madrid, 28040 Madrid, Spain.
Miguel
M
Vázquez-Velasco
Departamento de Nutrición y Bromatología I (Nutrición), Facultad de Farmacia, Universidad Complutense de Madrid, 28040 Madrid, Spain.
Laura
L
González-Torres
Departamento de Farmacología, Facultad de Farmacia, Universidad Complutense de Madrid, 28040 Madrid, Spain.
Patricia
P
López-Gasco
Departamento de Nutrición y Bromatología I (Nutrición), Facultad de Farmacia, Universidad Complutense de Madrid, 28040 Madrid, Spain.
Sara
S
Bastida
Departamento de Farmacología, Facultad de Farmacia, Universidad Complutense de Madrid, 28040 Madrid, Spain.
Juana
J
Benedí
Departamento de Bioquímica y Biología Molecular, Facultad de Farmacia, Universidad Complutense de Madrid, 28040 Madrid, Spain.
María Isabel
MI
Sánchez-Reus
Departamento de Nutrición, Bromatología y Toxicología, Facultad de Farmacia, Universidad de Alcalá, Alcalá de Henares, 28871 Madrid, Spain.
María José
MJ
González-Muñoz
Departamento de Nutrición y Bromatología I (Nutrición), Facultad de Farmacia, Universidad Complutense de Madrid, 28040 Madrid, Spain. Electronic address: frasan@ucm.es.
Francisco J
FJ
Sánchez-Muniz
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D007249:Inflammation / Q000517:prevention & control*; D008099:Liver / Q000378:metabolism*; D008297:Male; D008351:Mannans / Q000494:pharmacology*; D010084:Oxidation-Reduction; D051381:Rats; D011924:Rats, Zucker; D053545:Spirulina
D000975:Antioxidants; D008351:Mannans; C022901:(1-6)-alpha-glucomannan; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D005978:Glutathione
Antioxidants; Glucomannan; Glutathione system; Inflammation; Spirulina; Squid-surimi