Target-site mechanism of ACCase-inhibitors resistance in American sloughgrass (Beckmannia syzigachne Steud.) from China.
American sloughgrass (Beckmannia syzigachne) is a troublesome weed in winter wheat field rotated with rice in China. Fenoxaprop-p-ethyl and pinoxaden were observed failing to control American sloughgrass in the same filed in Lujiang county in 2011 and 2012, respectively. Whole-plant bioassay was conducted to determine the resistance to fenoxaprop-p-ethyl, pinoxaden and other herbicides in American sloughgrass. Dose-response experiment indicated that Lujiang population was highly resistant to fenoxaprop-p-ethyl (199.8-fold), pinoxaden (76.2-fold), clodinafop-propargyl (334.1-fold) and sethoxydim (15.9-fold); moderately resistant to clethodim (6.3-fold), susceptible to mesosulfuron-methyl, flucarbazone-sodium, pyroxsulam and isoproturon. Partial gene of CT domain was cloned and sequenced to confirm the molecular mechanism of resistance to ACCase-inhibiting herbicides. A Trp2027Cys mutation was found in Lujiang population according to the sequencing result. This mutation is the molecular mechanism of resistance to fenoxaprop-p-ethyl in Lujiang population. Furthermore the Trp2027Cys mutation very likely results in cross resistance to clodinafop-propargyl and pinoxaden in Lujiang population. 103 mutant homozygotes were detected from the 108 plants tested using a rapid dCAPS method developed in this paper. This is the first report of pinoxaden resistance and a mutation at position of 2027 for American sloughgrass.
Key Laboratory of Pesticide Toxicology and Application Technique, College of Plant Protection, Shandong Agricultural University, Tai'an 271018, Shandong, PR China; College of Chemistry and Pharmaceutical Science, Qingdao Agricultural University, Qingdao 266109, Shandong, PR China.
Lingxu
L
Li
Key Laboratory of Pesticide Toxicology and Application Technique, College of Plant Protection, Shandong Agricultural University, Tai'an 271018, Shandong, PR China.
Long
L
Du
Key Laboratory of Pesticide Toxicology and Application Technique, College of Plant Protection, Shandong Agricultural University, Tai'an 271018, Shandong, PR China.
Weitang
W
Liu
Key Laboratory of Pesticide Toxicology and Application Technique, College of Plant Protection, Shandong Agricultural University, Tai'an 271018, Shandong, PR China.
Guohui
G
Yuan
Key Laboratory of Pesticide Toxicology and Application Technique, College of Plant Protection, Shandong Agricultural University, Tai'an 271018, Shandong, PR China. Electronic address: wangjx@sdau.edu.cn.
Jinxin
J
Wang
D000103:Acetyl-CoA Carboxylase / Q000037:antagonists & inhibitors* / Q000235:genetics; D000595:Amino Acid Sequence; D002681:China; D003512:Cyclohexanones / Q000633:toxicity; D053209:Herbicide Resistance; D006540:Herbicides / Q000633:toxicity*; D006574:Heterocyclic Compounds, 2-Ring / Q000633:toxicity; D008129:Lolium / Q000187:drug effects* / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D010080:Oxazoles / Q000633:toxicity; D011422:Propionates / Q000633:toxicity; D011725:Pyridines / Q000633:toxicity
D003512:Cyclohexanones; D006540:Herbicides; D006574:Heterocyclic Compounds, 2-Ring; D010080:Oxazoles; D011422:Propionates; D011725:Pyridines; C441919:clethodim; C049437:sethoxydim; C416661:fenoxaprop ethyl; D000103:Acetyl-CoA Carboxylase; C561036:clodinafop-propargyl; C542519:pinoxaden
Beckmannia syzigachne; Fenoxaprop-p-ethyl; Mutation; Resistance; dCAPS