Functional state of myocardial mitochondria in ischemia reperfusion of the heart in rats adapted to hypoxia.
Parameters of respiration, transmembrane potential, and Ca(2+)-binding capacity of mitochondria isolated from Langendorff-perfused hearts of rats adapted to normobaric hypoxia were analyzed. Ischemia and reperfusion modeling in intact and adapted animals reduced Ca(2+)-binding capacity of mitochondria, which indicated increased sensitivity of mitochondrial permeability transition pores (MPTP) to calcium ions. These changes were accompanied by a decrease in transmembrane potential, ADP/O coefficient (ratio of added ADP to oxygen consumption in State 3), and inhibition of State 3 respiration. At the same time, adaptation attenuated the negative effect of ischemia and reperfusion on the functional state of mitochondria.
Research Institute of Cardiology, Siberian Division of the Russian Academy of Medical Sciences, Tomsk, Russia.
Yu B
YB
Lishmanov
N V
NV
Naryzhnaya
L N
LN
Maslov
E S
ES
Prokudina
A S
AS
Gorbunov
S Yu
SY
Tsibulnikov
D000222:Adaptation, Physiological; D000818:Animals; D002118:Calcium / Q000378:metabolism; D000860:Hypoxia / Q000503:physiopathology; D008297:Male; D053078:Membrane Potential, Mitochondrial; D008929:Mitochondria, Heart / Q000378:metabolism*; D033681:Mitochondrial Membrane Transport Proteins / Q000378:metabolism; D000083162:Mitochondrial Permeability Transition Pore; D015428:Myocardial Reperfusion Injury / Q000378:metabolism*; D010101:Oxygen Consumption; D017208:Rats, Wistar
D033681:Mitochondrial Membrane Transport Proteins; D000083162:Mitochondrial Permeability Transition Pore; D002118:Calcium