Stem cell adhesion and proliferation on hydrolyzed poly(butylene succinate)/β-tricalcium phosphate composites.
Although poly(butylene succinate)/β-tricalcium phosphate (PBSu/TCP) composites are biocompatible and allow the growth and osteogenic differentiation of stem cells, cell attachment and adhesion to the PBSu-based substrates is often limited. To enhance cell adhesion and proliferation, we used a sodium hydroxide (NaOH) hydrolysis technique to generate a different degree of roughness on PBSu/TCP substrates with different PBSu:TCP ratios. The results showed that NaOH hydrolysis increased surface roughness of PBSu/TCP substrates in a concentration-dependent manner. Substrates with higher ratios of TCP:PBSu provided more porous topography after NaOH hydrolysis, with a substrate containing 40 wt % TCP (PBSu/TCP-6040) hydrolyzed with 1.5M NaOH (HPBSu/TCP-6040-1.5) showing the highest degree of roughness. As with the roughness, PBSu/TCP surface hydrophilicity was positively affected by the increasing NaOH concentration and TCP incorporation. Stem cells adhered best on HPBSu/TCP-6040-1.5 with three-dimensionally elongated cell extensions. Moreover, the HPBSu/TCP-6040-1.5 substrate most significantly facilitated stem cell actin cytoskeleton reorganization and vinculin-positive focal adhesion formation when compared with the other substrates tested. HPBSu/TCP-6040-1.5 also demonstrated the greatest increase in cell proliferation when compared with the other substrates studied. In conclusion, the results have shown that among various substrates tested, HPBSu/TCP-6040-1.5 provided the best support for stem cell adhesion and proliferation, suggesting its potential use in bone engineering.
Thammasat University, Pathumthani, Thailand.
Somying
S
Patntirapong
Weerachai
W
Singhatanadgit
Preeyapan
P
Meesap
Tharinee
T
Theerathanagorn
Montree
M
Toso
Wanida
W
Janvikul
D002072:Butylene Glycols / Q000737:chemistry*; D002130:Calcium Phosphates / Q000737:chemistry*; D002448:Cell Adhesion; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D010012:Osteogenesis; D011108:Polymers / Q000737:chemistry*
D002072:Butylene Glycols; D002130:Calcium Phosphates; D011108:Polymers; C485817:beta-tricalcium phosphate; C089797:bionole
calcium phosphate; cell adhesion; cell proliferation; poly(butylene succinate); surface hydrolysis