The effect of 30% ethanolic extract of Indian propolis on replica of human dentin compared against commercially available desensitizing agent: A methodological SEM study in vitro.
OBJECTIVE
This study evaluated the ability of 30% ethanolic extract of Indian propolis on dentinal tubule occlusion comparatively against CPP-ACP containing desensitizing agent GC tooth mousse.


METHODS
The specimens were prepared from 30 freshly extracted sound human third molars stored in 10% formalin (pH 7.0) at a room temperature. From each specimen, a sectioned sample (5 mm length × 5 mm width × 3.5 mm depth) was obtained including the cervical area. Samples were smoothened and wet-polished with 1000- and 1200-grit aluminum oxide abrasive paper and diamond pastes, in order to stimulate the clinical aspect of hypersensitive dentin cervical surfaces. All the specimens were randomly assigned to three groups (n = 10), according to dentin surface treatments. Negative control: Untreated specimens (n = 4) and pretreated with 6% citric acid (n = 6); Test Group: 30% ethanolic extract of Indian propolis (n = 10); Positive Group: GC Tooth Mousse (n =10). All the specimens were prepared for SEM analysis.


RESULTS
GC tooth mousse promoted tubule occlusion by crystal-like deposits in the lumen of the tubules. While propolis created a thin, smooth layer over dentin surface.


CONCLUSIONS
According to the SEM analysis, both desensitizing agent were able to occlude the dentinal tubules.
Department of Public Health Dentistry, People's Dental Academy, People's University, Bhopal, Madhya Pradesh, India.
Sudhir
S
Hongal
Department of Public Health Dentistry, People's Dental Academy, People's University, Bhopal, Madhya Pradesh, India.
Nilesh Arjun
NA
Torwane
Department of Public Health Dentistry, People's Dental Academy, People's University, Bhopal, Madhya Pradesh, India.
Pankaj
P
Goel
Department of Public Health Dentistry, People's Dental Academy, People's University, Bhopal, Madhya Pradesh, India.
Byarakele
B
Chandrashekar
Dentin hypersensitivity; GC tooth mousse; dentinal tubule occlusion; desensitizing agents; propolis