Effect of Tamarindus indica L. leaves' fluid extract on human blood cells.
Tamarind leaves are edible; however, their saponin content could be toxic to human blood cells. In this article, the effect of tamarind leaf fluid extract (TFE) on human blood cells was evaluated by using several tests. Results revealed that TFE did not cause significant haemolysis on human red blood cells even at the lowest evaluated concentration (20 mg/mL). Blood protein denaturalisation ratio was consistently lower than in control at TFE concentrations greater than 40 mg/mL. Erythrocyte membrane damage caused by the action of oxidative H2O2 displayed a steady reduction with increasing TFE concentrations. In the reactive oxygen species (ROS) measurement by using flow cytometry assay, leucocyte viability was over 95% at tested concentrations, and a high ROS inhibition was also recorded. Protective behaviour found in TFE should be attributed to its polyphenol content. Thus, tamarind leaves can be regarded as a potential source of interesting phytochemicals.
a Pharmacy Department , Oriente University , Avenida Patricio Lumumba s/n, 90500 Santiago de Cuba , Cuba.
J C
JC
Escalona-Arranz
J
J
Garcia-Diaz
R
R
Perez-Rosés
J
J
De la Vega
J
J
Rodríguez-Amado
H J
HJ
Morris-Quevedo
D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D010084:Oxidation-Reduction; D010936:Plant Extracts / Q000097:blood / Q000737:chemistry; D018515:Plant Leaves / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D029861:Tamarindus / Q000737:chemistry*
D010936:Plant Extracts; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide
Tamarindus indica L.; leukocytes; polyphenols; red blood cells; saponins; toxic