Nuclear and cytoplasmic changes in erythrocytes of p53-deficient medaka fish (Oryzias latipes) after exposure to gamma-radiation.
Previous studies have examined the effects of gamma-radiation on Japanese fish, in particular medaka (Oryzias latipes). In the present work, alterations in erythrocytes were recorded as haematological bio-indicators of exposure to gamma-radiation. After exposure of medaka fish to two different doses of radiation (2 Gy and 10 Gy), many malformations in red blood cells were observed in the irradiated fish compared with control fish. These malformations included acanthocytes, crenated cells, amoeboid cells, and sickle cells. More malformations were seen at the higher radiation dose. No micronuclei were seen in any group, but nuclear abnormalities were observed. We conclude that gamma-radiation causes morphological malformations of erythrocytes and is harmful to medaka fish.
Zoology Department, Faculty of Science, Assiut University, 71516 Assiut, Egypt. Electronic address: alaa_h254@yahoo.com.
Alaa El-Din Hamid
Ael-D
Sayed
Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa, Chiba 277-8562, Japan.
Shoji
S
Oda
Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa, Chiba 277-8562, Japan.
Hiroshi
H
Mitani
D000818:Animals; D030801:Animals, Genetically Modified; D002467:Cell Nucleus / Q000528:radiation effects*; D048430:Cell Shape / Q000528:radiation effects; D003593:Cytoplasm / Q000528:radiation effects*; D004912:Erythrocytes / Q000166:cytology / Q000528:radiation effects* / Q000648:ultrastructure; D005720:Gamma Rays / Q000009:adverse effects; D053140:Organelle Shape / Q000528:radiation effects; D009990:Oryzias / Q000097:blood / Q000235:genetics; D011829:Radiation Dosage; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
D016159:Tumor Suppressor Protein p53
Erythrocytes; Gamma-radiation; Medaka; Micronucleus; p53