Potentiation of osteoclastogenesis by adipogenic conversion of bone marrow-derived mesenchymal stem cells.
Bone marrow-derived mesenchymal stem cells (BMSCs) are the indispensable component of the bone marrow, being the common precursors for adipocytes and osteoblasts. We show here that adipogenic differentiation resulted in increase in the production of adipocyte markers, such as adiponectin,fatty-acid binding proteins (FABP4), peroxisome proliferator-activated receptor γ (PPARγ), as well as the receptor activator of nuclear-κB ligand (RANKL). Co-culture of osteoclast precursors (OCPs) with BMSCs-derived adipocytes significantly enhanced osteoclast differentiation with low-dose RANKL, whose levels alone could not promote osteoclastogenesis. These results demonstrate for the first time that adipogenic differentiation of BMSCs plays a pivotal role in maintaining bone homeostasis.
Department of Radiation Medical Sciences, Atomic Bomb Disease Institute, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan.
Keisuke
K
Mori
Keiji
K
Suzuki
Akira
A
Hozumi
Hisataka
H
Goto
Masato
M
Tomita
Hironobu
H
Koseki
Shunichi
S
Yamashita
Makoto
M
Osaki
D017667:Adipocytes / Q000166:cytology*; D050156:Adipogenesis; D001862:Bone Resorption; D002454:Cell Differentiation; D002478:Cells, Cultured; D018920:Coculture Techniques; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism*; D010010:Osteoclasts / Q000166:cytology*; D010012:Osteogenesis; D053245:RANK Ligand / Q000235:genetics / Q000378:metabolism
D053245:RANK Ligand