Expression patterns of micro-RNAs 146a, 181a, and 155 in subacute sclerosing panencephalitis.
Subacute sclerosing panencephalitis is caused by persistent brain infection of mutated virus, showing inflammation, neurodegeneration, and demyelination. Although many factors are emphasized in the pathogenesis of subacute sclerosing panencephalitis, the exact mechanism of neurodegeneration remains unknown. Micro-RNAs are small, noncoding RNAs that regulate gene expression at the posttranscriptional levels. Micro-RNAs are essential for normal immune system development; besides they are also implicated in the pathogenesis of many chronic inflammatory disorders. The aim of this study is to investigate the expression patterns of micro-RNAs 146a, 181a, and 155 in peripheral blood mononuclear cells of patients with subacute sclerosing panencephalitis. We enrolled 39 patients with subacute sclerosing panencephalitis and 41 healthy controls. Quantitative analysis of micro-RNAs 146a, 181a, and 155 were performed using specific stem-loop primers followed by real-time polymerase chain reaction. All of 3 micro-RNAs were upregulated in subacute sclerosing panencephalitis patients. In addition, the level of micro-RNA 155 expression was higher in stage 3 patients. But, micro-RNA 146a and 181a expression levels showed no association or correlation with clinically relevant data. Alteration of peripheral blood mononuclear cell micro-RNAs in subacute sclerosing panencephalitis may shed new light on the pathogenesis of disease and may contribute to the aberrant systemic rise in mRNA levels in subacute sclerosing panencephalitis.
School of Medicine, Department of Pediatrics, Division of Child Neurology, Dokuz Eylül University, İzmir, Turkey ulyis@yahoo.com.
Uluç
U
Yiş
Health Science Institute, Department of Neuroscience, Dokuz Eylül University, İzmir, Turkey.
Uğur Kemal
UK
Tüfekçi
Health Science Institute, Department of Neuroscience, Dokuz Eylül University, İzmir, Turkey.
Şermin
Ş
Genç
Gaziantep Children's Hospital, Division of Child Neurology, Gaziantep, Turkey.
Kürşat Bora
KB
Çarman
School of Medicine, Department of Pediatrics, Division of Child Neurology, Dokuz Eylül University, İzmir, Turkey.
Erhan
E
Bayram
School of Medicine, Department of Pediatrics, Division of Child Neurology, Dokuz Eylül University, İzmir, Turkey.
Yasemin
Y
Topçu
School of Medicine, Department of Pediatrics, Division of Child Neurology, Dokuz Eylül University, İzmir, Turkey.
Semra Hız
SH
Kurul
D000293:Adolescent; D002648:Child; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D013344:Subacute Sclerosing Panencephalitis / Q000235:genetics* / Q000378:metabolism*; D015854:Up-Regulation / Q000235:genetics*
C542542:MIRN146 microRNA, human; C524942:MIRN155 microRNA, human; C540193:MIrn181 microRNA, human; D035683:MicroRNAs
micro-RNA 146a; micro-RNA 155; micro-RNA 181a; subacute sclerosing panencephalitis