Encapsulation of rutin and naringenin in multilamellar vesicles for optimum antioxidant activity.
Rutin and naringenin, two phenolic compounds with antioxidant properties were encapsulated in lipid-based onion-type multilamellar vesicles (MLVs). After vesicles formation, the free, adsorbed/encapsulated analytes were well separated with size exclusion chromatography (SEC), and rutin and naringenin were quantified with UV-HPLC at 258 nm and 290 nm. A mathematical model was developed to separately calculate the encapsulation and the adsorption yields of both phenols. Naringenin was shown to be poorly encapsulated (<10%) but highly adsorbed on MLVs surface (>60%) whatever MLVs composition. Conversely, rutin showed high encapsulation efficiency (>60%). Entrapment of rutin was proved to be efficient since no leak was observed within 30 days in concentrated MLVs phase, while 16.0±0.3% of rutin was still encapsulated after 30 days when MLVs were diluted in water. Free rutin broke up into quercetin while the encapsulated one remained stable. DPPH assay confirmed that only free and adsorbed rutin participated in antioxidant activity.
S.O.F.I.A. Cosmétiques, 1ère Avenue, 1ère Rue, 06514 Carros, France; Université Nice Sophia Antipolis, ICN, UMR 7272, Parc Valrose, 06108 Nice CEDEX 2, France.
Audrey
A
Kerdudo
S.O.F.I.A. Cosmétiques, 1ère Avenue, 1ère Rue, 06514 Carros, France.
Alexandre
A
Dingas
Université Nice Sophia Antipolis, ICN, UMR 7272, Parc Valrose, 06108 Nice CEDEX 2, France.
Xavier
X
Fernandez
Laboratoire de Chimie et Biologie des Membranes et Nano-objets, Univ. Bordeaux, CBMN, UMR 5248, Allée Geoffroy St. Hilaire, F-33600 Pessac, France. Electronic address: chrystel.faure@enscbp.fr.
Chrystel
C
Faure
D000327:Adsorption; D000975:Antioxidants / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D016503:Drug Delivery Systems; D044950:Flavanones / Q000008:administration & dosage* / Q000032:analysis / Q000494:pharmacology; D008081:Liposomes; D008962:Models, Theoretical; D012431:Rutin / Q000008:administration & dosage* / Q000032:analysis / Q000494:pharmacology
D000975:Antioxidants; D044950:Flavanones; D008081:Liposomes; D012431:Rutin; C005273:naringenin
Antioxidant activity; Encapsulation; Flavonoids; Multilamellar vesicles