Water-insoluble fiber-rich fraction from pineapple peel improves intestinal function in hamsters: evidence from cecal and fecal indicators.
Pineapple peel, a byproduct of agricultural processing, contains high levels of water-insoluble fiber-rich fraction (WIFF) (~42%, wt/wt). Our previous work has demonstrated that cellulose, hemicellulose (xylan and xyloglucan), and pectic substances are the major polysaccharides of pineapple-peel WIFF. Based on its chemical composition and unique characteristics, we hypothesized that daily consumption of WIFF would improve intestinal function in hamsters. Male Golden Syrian hamsters were fed a diet supplemented with either 5% cellulose or various amounts of WIFF (2.5%, 5%, or 10%). Activities of fecal bacterial enzymes, short-chain fatty acid concentrations, and microbial number in the cecal content, and also biochemical indicators in the cecal and feces of hamsters, were evaluated in all groups. The supplementation of WIFF in a diet at a level of 2.5% significantly (P < .05) decreased the daily fecal ammonia output; shortened the gastrointestinal transit time; reduced the activities of β-D-glucosidase, β-D-glucuronidase, mucinase, and urease in feces; and also enhanced the total amounts of short-chain fatty acid in the cecal content and the growth of gut microflora such as Lactobacillus spp and Bifidobacterium spp. These results indicate that WIFF could improve cecal ecosystem function of hamsters by reducing the toxic compounds excreted by intestinal microflora. Therefore, pineapple-peel WIFF could be a promising candidate for a functional ingredient beneficial to human intestinal function and health.
Department of Seafood Science, National Kaohsiung Marine University, 142, Hai-Chuan Road, Nan-Tzu, Kaohsiung 81157, Taiwan. Electronic address: ylhuang@mail.nkmu.edu.tw.
Ya-Ling
YL
Huang
Department of Seafood Science, National Kaohsiung Marine University, 142, Hai-Chuan Road, Nan-Tzu, Kaohsiung 81157, Taiwan.
Yung-Hsiang
YH
Tsai
Department of Seafood Science, National Kaohsiung Marine University, 142, Hai-Chuan Road, Nan-Tzu, Kaohsiung 81157, Taiwan.
Chau-Jen
CJ
Chow
D000641:Ammonia / Q000378:metabolism; D031230:Ananas; D000818:Animals; D001419:Bacteria / Q000201:enzymology / Q000254:growth & development; D001644:Bifidobacterium / Q000254:growth & development; D002432:Cecum / Q000187:drug effects* / Q000378:metabolism / Q000382:microbiology; D006224:Cricetinae; D003672:Defecation; D004040:Dietary Carbohydrates / Q000494:pharmacology*; D004043:Dietary Fiber / Q000494:pharmacology*; D019587:Dietary Supplements; D005232:Fatty Acids, Volatile / Q000378:metabolism; D005243:Feces / Q000201:enzymology / Q000382:microbiology; D005638:Fruit; D005772:Gastrointestinal Transit / Q000187:drug effects; D007420:Intestine, Large / Q000187:drug effects* / Q000382:microbiology / Q000502:physiology; D007778:Lactobacillus / Q000254:growth & development; D008297:Male; D008647:Mesocricetus; D010936:Plant Extracts / Q000494:pharmacology; D011134:Polysaccharides / Q000494:pharmacology; D012995:Solubility
D004040:Dietary Carbohydrates; D004043:Dietary Fiber; D005232:Fatty Acids, Volatile; D010936:Plant Extracts; D011134:Polysaccharides; D000641:Ammonia
Bacterial enzyme; Hamsters; Intestinal health; Intestinal microflora; Peel; Pineapple; Short-chain fatty acids