[Correlation and clinical significance of expressions of HIF-1α and Sema4D in colorectal carcinoma tissues].
OBJECTIVE
To compare the expressions of hypoxia-inducible factor 1 alpha(HIF-1α) and Semaphorin 4D(Sema4D) in colorectal carcinoma and normal colorectal tissues, and to investigate their correlation and clinical significance.


METHODS
The expressions of HIF-1α and Sema4D were examined in 86 cases of colorectal carcinoma and 52 normal colorectal tissues by SP immunohistochemical staining. Correlation between these two expressions and association of the expressions with clinicopathological characters and prognosis were analyzed.


RESULTS
The positive rates of HIF-1α and Sema4D protein in colorectal carcinoma tissues were significantly higher than those in normal colorectal tissues(58.1% vs. 7.7%, χ(2)=34.624, P<0.01; 60.5% vs. 11.5%, χ(2)=31.839, P<0.01). HIF-1α and Sema4D protein expressions were closely associated with colorectal carcinoma histological types(P=0.003, P=0.010), TNM staging (P=0.003, P=0.017) and lymphatic metastasis (P=0.003, P=0.020), and a significant correlation was observed between the expressions of HIF-1α and Sema4D protein (r=0.567, P<0.01). The 5-year overall survival rate was 37%. Univariate analysis showed that 5-year survival rates of patients with positive and negative HIF-1α protein expression were 24% and 56%(P=0.003), and those with positive and negative Sema4D protein expression were 23% and 59%(P=0.001). Multivariate Cox analysis showed that expression of Sema4D was an independent prognostic factor of colorectal cancer patients(P=0.026), while expression of HIF-1α was not(P=0.501).


CONCLUSIONS
Combined detection of HIF-1α and Sema4D has the potential to predict the development trend of colorectal carcinoma and prognosis of patients.
Department of Gastrointestinal Surgery, Provincial Hospital, Shandong University, Jinan 250021, China. jing66510122@sina.com.
Linjun
L
Mu
Jinshen
J
Wang
Xiaobo
X
Guo
Sheng
S
Zheng
Keshu
K
Shan
Changqing
C
Jing
Leping
L
Li
D015703:Antigens, CD / Q000378:metabolism*; D015179:Colorectal Neoplasms / Q000378:metabolism*; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism*; D008207:Lymphatic Metastasis; D009367:Neoplasm Staging; D011379:Prognosis; D039961:Semaphorins / Q000378:metabolism*; D015996:Survival Rate
D015703:Antigens, CD; C093224:CD100 antigen; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D039961:Semaphorins