Phenolic lipids affect the activity and conformation of acetylcholinesterase from Electrophorus electricus (Electric eel).
Phenolic lipids were isolated from rye grains, cashew nutshell liquid (CNSL) from Anacardium occidentale, and fruit bodies of Merrulius tremellosus, and their effects on the electric eel acetylcholinesterase activity and conformation were studied. The observed effect distinctly depended on the chemical structure of the phenolic lipids that were available for interaction with the enzyme. All of the tested compounds reduced the activity of acetylcholinesterase. The degree of inhibition varied, showing a correlation with changes in the conformation of the enzyme tested by the intrinsic fluorescence of the Trp residues of the protein.
Department of Lipids and Liposomes, Faculty of Biotechnology, University of Wroclaw, Joliot-Curie 14a, Wroclaw 50-383, Poland. stasiuk@ibmb.uni.wroc.pl.
Maria
M
Stasiuk
Department of Lipids and Liposomes, Faculty of Biotechnology, University of Wroclaw, Joliot-Curie 14a, Wroclaw 50-383, Poland. ala_j@vp.pl.
Alicja
A
Janiszewska
Department of Lipids and Liposomes, Faculty of Biotechnology, University of Wroclaw, Joliot-Curie 14a, Wroclaw 50-383, Poland. kozubek@ibmb.uni.wroc.pl.
Arkadiusz
A
Kozubek
D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D002800:Cholinesterase Inhibitors / Q000737:chemistry / Q000494:pharmacology; D004593:Electrophorus / Q000378:metabolism*; D029941:Fish Proteins / Q000378:metabolism*; D008055:Lipids / Q000737:chemistry / Q000494:pharmacology*; D010636:Phenols / Q000737:chemistry / Q000494:pharmacology*
D002800:Cholinesterase Inhibitors; D029941:Fish Proteins; D008055:Lipids; D010636:Phenols; D000110:Acetylcholinesterase