Decreased level of albumin in peripheral blood mononuclear cells of streptozotocin-induced diabetic rats.
We investigated the phenotypic level of albumin in peripheral blood mononuclear cells (PBMC) of streptozotocin (STZ)-induced diabetic rats. A specific reduction of albumin was identified by 2-dimensional electrophoresis and mass spectrometry. Decreased albumin content was also confirmed by immunoblotting and quantitative real-time PCR. Since albumin is a major and predominant antioxidant in plasma, the PBMC albumin may also contribute to their antioxidant activity. By measuring the amount of H2O2, lipid peroxidation and the redox form of glutathione, it was found that the production of the oxidative stress was elevated in STZ-diabetic rats compared to that of normal control. We suggest, therefore, that decreased albumin content may lead to the decreased antioxidant activity in the PBMC of type 1 diabetic rats.
School of Biological Sciences and Technology, Chonnam National University, Gwangju 500-757, Republic of Korea.
Ki Tae
KT
Park
Chul-Ho
CH
Yun
Chun-Sik
CS
Bae
Taeho
T
Ahn
D000418:Albumins / Q000032:analysis*; D000818:Animals; D017931:DNA Primers / Q000235:genetics; D003921:Diabetes Mellitus, Experimental / Q000097:blood* / Q000503:physiopathology; D015180:Electrophoresis, Gel, Two-Dimensional; D005978:Glutathione / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000737:chemistry*; D015227:Lipid Peroxidation / Q000502:physiology; D008297:Male; D013058:Mass Spectrometry; D018384:Oxidative Stress / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction
D000418:Albumins; D017931:DNA Primers; D006861:Hydrogen Peroxide; D005978:Glutathione