Analysis of carotenoids and tocopherols in plant matrices and assessment of their in vitro antioxidant capacity.
Carotenoids and tocopherols are lipid secondary metabolites that play essential roles in plants. They are also relevant compounds from a nutritional standpoint and attract much attention due to their proposed antioxidant properties. In this chapter, methodologies for the extraction and HPLC analysis of these compounds are described as well as a widely used protocol to assess their antioxidant capacity.
Facultad de Farmacia, Department of Nutrition & Food Science, Food Colour & Quality Laboratory, Universidad de Sevilla, P. García González 2, 41012, Sevilla, Spain, ajmelendez@us.es.
Antonio J
AJ
Meléndez-Martínez
Carla M
CM
Stinco
Paula Mapelli
PM
Brahm
Isabel M
IM
Vicario
D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D002338:Carotenoids / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D005591:Chemical Fractionation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D066298:In Vitro Techniques / Q000379:methods* / Q000592:standards; D010944:Plants / Q000737:chemistry*; D012015:Reference Standards; D024505:Tocopherols / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*
D000975:Antioxidants; D002338:Carotenoids; D024505:Tocopherols