Structure of the capsular polysaccharide of Acinetobacter baumannii ACICU containing di-N-acetylpseudaminic acid.
Capsular polysaccharide was isolated by the phenol-water extraction of Acinetobacter baumannii ACICU cells and studied by sugar analysis, partial acid hydrolysis, and 1D and 2D (1)H and (13)C NMR spectroscopy. The polysaccharide was found to contain 5,7-diacetamido-3,5,7,9-tetradeoxy-l-glycero-l-manno-non-2-ulosonic or di-N-acetylpseudaminic acid (Pse5Ac7Ac), and the following structure of the branched tetrasaccharide repeating unit was established: The genes present in the polysaccharide gene cluster of A. baumannii ACICU are appropriate to the structure established.
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Sof'ya N
SN
Senchenkova
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Alexander S
AS
Shashkov
M. M. Shemyakin & Y. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Mikhail M
MM
Shneider
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Nikolay P
NP
Arbatsky
State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, Russia.
Anastasiya V
AV
Popova
M. M. Shemyakin & Y. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Konstantin A
KA
Miroshnikov
State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, Russia.
Nikolay V
NV
Volozhantsev
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia. Electronic address: yknirel@gmail.com.
Yuriy A
YA
Knirel
D040981:Acinetobacter baumannii / Q000737:chemistry*; D002236:Carbohydrate Conformation; D006868:Hydrolysis; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification; D012794:Sialic Acids / Q000737:chemistry*
C502287:5,7-diacetamido-3,5,7,9-tetradeoxynon-2-ulosonic acid; D011135:Polysaccharides, Bacterial; D012794:Sialic Acids
Acinetobacter baumannii; Capsular polysaccharide structure; Polysaccharide gene cluster; Pseudaminic acid