Genome-wide analysis of terpene synthases in soybean: functional characterization of GmTPS3.
Terpenes (terpenoids or isoprenoids) constitute a large class of plant natural products and play numerous functional roles in primary and secondary metabolism as well as inecological interactions. This study presents a genomic analysis of 23 putative soybean (Glycine max) terpene synthase genes (GmTPSs) distributed over 10 of 20 chromosomes. The GmTPSs are grouped into six types based on gene architecture and sequence identity. Sequence alignment indicates that most GmTPSs contain the conserved aspartate-rich DDX2D motif, and two clades encoded by TPS-a and TPS-b contain variations of an arginine-rich RRX8W motif. Quantitative real-time PCR analysis demonstrated that GmTPSs were predominantly expressed in reproductive organs. Heterologous expression followed by enzymatic assay suggested that GmTPS3 functions as a geraniol synthase. We also generated transgenic tobacco plants ectopically expressing GmTPS3. In dual-choice feeding-preference and force-feeding assays, the transgenic tobacco lines expressing GmTPS3 exhibited enhanced resistance to cotton leafworms and an increased level of geraniol. Taken together, these data provide a comprehensive understanding of the TPS family in soybeans and suggest a promising approach to engineering transgenic plants with enhanced insect resistance.
National Center for Soybean Improvement, National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Weigang 1, Nanjing 210095, China; Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, National Engineering Research Center of Edible Fungi, Shanghai 201403, China. Electronic address: sxauljy@163.com.
Jianyu
J
Liu
National Center for Soybean Improvement, National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Weigang 1, Nanjing 210095, China. Electronic address: fhuang@njau.edu.cn.
Fang
F
Huang
National Center for Soybean Improvement, National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Weigang 1, Nanjing 210095, China. Electronic address: amethyst627@sina.com.
Xia
X
Wang
Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China. Electronic address: fldzm04@126.com.
Man
M
Zhang
College of Life Science, Ningxia University, Yinchuan 750021, China. Electronic address: xlzheng@126.com.
Rui
R
Zheng
National Center for Soybean Improvement, National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Weigang 1, Nanjing 210095, China. Electronic address: wjiao@njau.edu.cn.
Jiao
J
Wang
National Center for Soybean Improvement, National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Weigang 1, Nanjing 210095, China. Electronic address: dyyu@njau.edu.cn.
Deyue
D
Yu
D000080462:Acyclic Monoterpenes; D019883:Alkyl and Aryl Transferases / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D060467:Disease Resistance / Q000235:genetics; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D018745:Genome, Plant / Q000235:genetics*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006790:Host-Parasite Interactions; D007313:Insecta / Q000502:physiology; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D018613:Microscopy, Confocal; D005810:Multigene Family; D010802:Phylogeny; D010935:Plant Diseases / Q000235:genetics / Q000469:parasitology; D010940:Plant Proteins / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D018087:Plastids / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D030262:Soybean Proteins / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D013025:Glycine max / Q000201:enzymology / Q000235:genetics*; D013314:Stress, Mechanical; D013729:Terpenes / Q000378:metabolism; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism / Q000469:parasitology
D000080462:Acyclic Monoterpenes; D007527:Isoenzymes; D010940:Plant Proteins; D030262:Soybean Proteins; D013729:Terpenes; D049452:Green Fluorescent Proteins; D019883:Alkyl and Aryl Transferases; C108877:terpene synthase; C007836:geraniol
Cotton leafworms; Geraniol synthase; Insect resistance; Soybean; Terpene synthase