Development and clinical evaluation of sdaA loop-mediated isothermal amplification assay for detection of Mycobacterium tuberculosis with an approach to prevent carryover contamination.
A rapid and sensitive loop-mediated isothermal amplification assay for the sdaA gene of Mycobacterium tuberculosis was developed using a dUTP-uracil-N-glycosylase (dUTP-UNG) strategy to prevent carryover contamination. Evaluation of the assay using clinical specimens (n = 648) showed high specificity (97.2%) and sensitivity (100%), demonstrating its potential as a diagnostic test for tuberculosis, especially in resource-limited settings.
Dr BR Ambedkar Center for Biomedical Research, University of Delhi, Delhi, India Shri Guru Tegh Bahadur Khalsa College, University of Delhi, Delhi, India.
Manoj
M
Nimesh
Dr BR Ambedkar Center for Biomedical Research, University of Delhi, Delhi, India.
Deepali
D
Joon
Department of Microbiology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi, India.
Mandira
M
Varma-Basil
Dr BR Ambedkar Center for Biomedical Research, University of Delhi, Delhi, India dsalujach59@gmail.com.
Daman
D
Saluja
D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012680:Sensitivity and Specificity; D013696:Temperature; D013997:Time Factors; D014376:Tuberculosis / Q000175:diagnosis*