Modulation of ovarian steroidogenesis by adiponectin during delayed embryonic development of Cynopterus sphinx.
The aim of present study was to evaluate role of adiponectin in ovarian steroidogenesis during delayed embryonic development of Cynopterus sphinx. This study showed significantly low circulating adiponectin level and a decline in expression of adiponectin receptor 1 (AdipoR1) in the ovary during the period of delayed embryonic development as compared with the normal development. The adiponectin treatment in vivo during the period of delayed development caused significantly increased in circulating progesterone and estradiol levels together with increased expression of AdipoR1 in the ovary. The in vitro study confirmed the stimulatory effect of adiponectin on progesterone synthesis. Both in vivo and in vitro studies showed that the effects of adiponectin on ovarian steroidogenesis were mediated through increased expression of luteinizing hormone-receptor, steroidogenic acute regulatory protein and 3β-hydroxyl steroid dehydrogenase enzyme. The adiponectin treatment may also promote progesterone synthesis by modulating ovarian angiogenesis, cell survival and rate of apoptosis.
Department of Zoology, Banaras Hindu University, Varanasi 221 005, India.
Anuradha
Department of Zoology, Banaras Hindu University, Varanasi 221 005, India. Electronic address: akrishna_ak@yahoo.co.in.
Amitabh
A
Krishna
D052242:Adiponectin / Q000494:pharmacology*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D015153:Blotting, Western; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D002685:Chiroptera; D004622:Embryo, Mammalian / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D047108:Embryonic Development / Q000187:drug effects* / Q000502:physiology; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D007124:Immunoenzyme Techniques; D010053:Ovary / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D011247:Pregnancy; D011374:Progesterone / Q000378:metabolism*; D054419:Receptors, Adiponectin / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects
D052242:Adiponectin; D054419:Receptors, Adiponectin; D011374:Progesterone; D004958:Estradiol
AdipoR1; Adiponectin; Corpus luteum; Delayed embryonic development; Progesterone