Holographic tracking of living cells by three-dimensional reconstructed complex wavefronts alignment.
We propose here a new three-dimensional (3D) holographic tracking method capable to track, simultaneously and in a single step, all the spatial coordinates of micro-objects. The approach is based on the enhanced correlation coefficient (ECC) maximization method but applied, for the first time to the best of our knowledge, directly on the holographic reconstructed complex wave fields. The key novelty of the proposed strategy is its ability to calculate simultaneously the 3D coordinates of cells, without decoupling the contribution of amplitude and phase. The proposed strategy is tested on living cells (i.e., NIH 3T3 mouse fibroblast) flowing into a microfluidic channel and compared with classical holographic tracking approach. Theoretical description and experimental validation of the proposed strategy are reported.
Pasquale
P
Memmolo
Lisa
L
Miccio
Andrea
A
Finizio
Paolo A
PA
Netti
Pietro
P
Ferraro
D000465:Algorithms; D000818:Animals; D058948:Cell Tracking / Q000295:instrumentation / Q000379:methods*; D006696:Holography / Q000295:instrumentation / Q000379:methods*; D007089:Image Enhancement / Q000295:instrumentation / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000295:instrumentation / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000295:instrumentation / Q000379:methods*; D051379:Mice; D008954:Models, Biological; D015233:Models, Statistical; D041681:NIH 3T3 Cells; D010363:Pattern Recognition, Automated / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013382:Subtraction Technique