Rapid electrodeposition of a gold-Prussian blue nanocomposite with ultrahigh electroactivity for dual-potential amperometric biosensing of uric acid.
We report a new and efficient protocol for rapid electrodeposition of a gold-Prussian blue ((Au-PB)REd) nanocomposite with ultrahigh electroactivity on an Au electrode by cyclic voltammetry in 0.1 M aqueous K2SO4 containing 1 mM K3Fe(CN)6, 1 mM HAuCl4, and 0.1 mM Fe2(SO4)3, and an electrochemical quartz crystal microbalance and scanning electron microscopy were utilized to investigate the electrodeposition. The (Au-PB)REd/Au electrode was then cast-coated with a urate oxidase (UOx)-poly(anilineboronic acid) (PABA)-Pt nanoparticle (PtNP) bionanocomposite and chitosan (CS) for high-performance amperometric biosensing of uric acid (UA) in the dual-potential mode. The UOx-PABA-PtNP bionanocomposite was prepared through chemical oxidation of anilineboronic acid (ABA) by sodium chloroplatinate in the presence of UOx. The thus-fabricated CS/UOx-PABA-PtNP/(Au-PB)REd/Au enzyme electrode worked well under optimized conditions through both oxidation and reduction determination of enzyme-generated H2O2, which responded linearly to UA concentrations from 0.3 μM to 0.65 mM with a sensitivity of 223 μA mM(-1) cm(-2) and a limit of detection (LOD) of 0.2 μM (0.7 V vs. SCE), and from 0.2 μM to 0.25 mM with a sensitivity of 247 μA mM(-1) cm(-2) and a LOD of 0.1 μM (-0.05 V vs. SCE), being superior to most analogues hitherto reported for biosensing of UA.
Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research (Ministry of Education of China), National & Local Joint Engineering Laboratory for New Petro-chemical Materials and Fine Utilization of Resources, College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, China. xieqj@hunnu.edu.cn chenchao840103@163.com.
Wen
W
Wang
Cong
C
Qin
Qingji
Q
Xie
Xiaoli
X
Qin
Long
L
Chao
Yi
Y
Huang
Mengzhen
M
Dai
Chao
C
Chen
Jianying
J
Huang
Jiming
J
Hu
D015374:Biosensing Techniques; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D005295:Ferrocyanides / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D053761:Nanocomposites; D014527:Uric Acid / Q000032:analysis*
D005295:Ferrocyanides; D014527:Uric Acid; D006046:Gold; C000170:ferric ferrocyanide