Urinary metabolomic study of non-small cell lung carcinoma based on ultra high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry.
Metabolic profiles from human urine reveal the significant difference of carnitine and acylcarnitines levels between non-small cell lung carcinoma patients and healthy controls. Urine samples from cancer patients and healthy individuals were assayed in this metabolomic study using ultra high performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry. The data were normalized by the sum of all intensities and creatinine calibration, respectively, before orthogonal partial least squares discriminant analysis. Twenty differential metabolites were identified based on standard compounds or tandem mass spectrometry fragments. Among them, some medium-/long-chain acylcarnitines, for example, cis-3,4-methylene heptanoylcarnitine, were found to be downregulated while carnitine was upregulated in urine samples from the cancer group compared to the control group. Receiver operating characteristic analysis of the two groups showed that the area under curve for the combination of carnitine and 11 selected acylcarnitines was 0.958. This study suggests that the developed carnitine and acylcarnitines profiling method has the potential to be used for screening non-small cell lung carcinoma.
School of Pharmacy, Shanghai Jiao Tong University, Shanghai, China.
Qian
Q
Wu
Yan
Y
Wang
Xue
X
Gu
Junyi
J
Zhou
Huiping
H
Zhang
Wang
W
Lv
Zhe
Z
Chen
Chao
C
Yan
D000328:Adult; D000368:Aged; D002331:Carnitine / Q000031:analogs & derivatives / Q000378:metabolism / Q000652:urine; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism / Q000652:urine*; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D055432:Metabolomics / Q000379:methods*; D008875:Middle Aged; D055752:Small Cell Lung Carcinoma / Q000378:metabolism / Q000652:urine*; D014556:Urine / Q000737:chemistry*
C116917:acylcarnitine; D002331:Carnitine
Acylcarnitines; Lung cancer; Mass spectrometry; Metabolomics; Urine