Effect of umbilical cord serum coated 3D PCL/alginate scaffold for mastoid obliteration.
OBJECTIVE
Human umbilical cord serum (hUCS) has a lot of growth factors. To date, there are no reports on stimulating effect of hUCS in osteogenesis. The purpose of this study is to evaluate enhancing effect of hUCS in osteogenesis by mastoid obliteration combined with bony scaffold.


METHODS
The fabrication procedure for obtaining PCL/alginate/hUCS was performed. The bulla obliteration was done using PCL/alginate/hUCS in the experimental group and PCL in the control group. To assess the early active mineralization of new bone formation, guinea pigs of each group received an intraperitoneal infusion with alizarin red at 6 weeks post-surgery. The animals of each group were sacrificed 8 weeks post-surgery. Ex vivo microCT and histologic observation were performed.


RESULTS
MicroCT finding shows more radiopaque change within the pores in the experimental group compared to the control group. Stereomicroscopic and SEM findings show new bone formation of the pores in the experimental group. However, the pores between strands almost all remained in the control group. Corresponding histological observations for the stimulatory effects of hUCS showed osteogenesis in the pores between the strands compared to the control group.


CONCLUSIONS
Our data suggest that hUCS coated 3D porous PCL scaffold in mastoid obliteration provides enhanced osteogenesis. Therefore, we suggest that our hUCS coated 3D porous PCL could be used in mastoid obliteration in the future.
Department of Otolaryngology, Chonnam National University Medical School, Gwangju, South Korea; Research Center for Resistant Cells, Chosun University, Gwangju, South Korea. Electronic address: chulsavio@hanmail.net.
Chul Ho
CH
Jang
Department of Otolaryngology, Chonnam National University Medical School, Gwangju, South Korea.
Yong Beom
YB
Cho
Research Center for Resistant Cells, Chosun University, Gwangju, South Korea; Department of Bio New Drug Development, Chosun University, Gwangju, South Korea.
Cheol Hee
CH
Choi
Department of Bio New Drug Development, Chosun University, Gwangju, South Korea.
Yoon Seok
YS
Jang
Department of Biomedical Engineering, and Center for Marine-integrated Biomedical Technology (BK 21 Plus), Pukyung National University, Busan, South Korea.
Won-Kyo
WK
Jung
Department of Bio-Mechatronic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon, South Korea.
Hyeongjin
H
Lee
Department of Bio-Mechatronic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon, South Korea. Electronic address: xrdghk@gmail.com.
Geun Hyung
GH
Kim
D000464:Alginates / Q000737:chemistry; D000818:Animals; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D005312:Fetal Blood / Q000737:chemistry*; D006168:Guinea Pigs; D006801:Humans; D008297:Male; D008416:Mastoid / Q000473:pathology / Q000601:surgery*; D008855:Microscopy, Electron, Scanning; D010012:Osteogenesis; D011091:Polyesters / Q000737:chemistry; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*; D055114:X-Ray Microtomography
D000464:Alginates; D020099:Coated Materials, Biocompatible; D011091:Polyesters; C016240:polycaprolactone
Mastoid obliteration; Osteogenesis; Polycaprolactone; Umbilical cord serum