A proteomic view to characterize the effect of chitosan nanoparticle to hepatic cells: is chitosan nanoparticle an enhancer of PI3K/AKT1/mTOR pathway?
Chitosan nanoparticle, a biocompatible material, was used as a potential drug delivery system widely. Our current investigation studies were the bioeffects of the chitosan nanoparticle uptake by liver cells. In this experiment, the characterizations of chitosan nanoparticles were measured by transmission electron microscopy and particle size analyzer. The average size of the chitosan nanoparticle was 224.6 ± 11.2 nm, and the average zeta potential was +14.08 ± 0.7 mV. Moreover, using proteomic approaches to analyze the differential protein expression patterns resulted from the chitosan nanoparticle uptaken by HepG2 and CCL-13 cells identified several proteins involved in the PI3K/AKT1/mTOR pathway. Our experimental results have demonstrated that the chitosan nanoparticle may involve in the liver cancer cell metastasis and proliferation.
Instrument Technology Research Center, National Applied Research Laboratories, Hsinchu 300, Taiwan ; Translational Research Center, Kaohsiung Medical University Chung-Ho Memorial Hospital, Kaohsiung 807, Taiwan ; Department of Medical Research, Kaohsiung Medical University Chung-Ho Memorial Hospital, Kaohsiung 807, Taiwan.
Ming-Hui
0000-0001-5856-0498
MH
Yang
Translational Research Center, Kaohsiung Medical University Chung-Ho Memorial Hospital, Kaohsiung 807, Taiwan ; Department of Medical Research, Kaohsiung Medical University Chung-Ho Memorial Hospital, Kaohsiung 807, Taiwan ; Department of Obstetrics and Gynecology, Kaohsiung Medical University Chung-Ho Memorial Hospital, Kaohsiung 807, Taiwan ; School of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
Shyng-Shiou
SS
Yuan
Department of Medical Imaging and Radiological Sciences, Kaohsiung Medical University, Kaohsiung 807, Taiwan ; Department of Nuclear Medicine, Kaohsiung Medical University Chung-Ho Memorial Hospital, Kaohsiung 807, Taiwan.
Ying-Fong
YF
Huang
Department of Chemistry, National Sun Yat-sen University, Kaohsiung 804, Taiwan ; National Sun Yat-sen University-Kaohsiung Medical University Joint Research Center, Kaohsiung 807, Taiwan.
Po-Chiao
PC
Lin
National Sun Yat-sen University-Kaohsiung Medical University Joint Research Center, Kaohsiung 807, Taiwan ; Department of Biochemistry, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
Chi-Yu
CY
Lu
Department of Biomedical Engineering, National Yang-Ming University, Taipei 112, Taiwan.
Tze-Wen
0000-0002-7469-9913
TW
Chung
Translational Research Center, Kaohsiung Medical University Chung-Ho Memorial Hospital, Kaohsiung 807, Taiwan ; Department of Medical Imaging and Radiological Sciences, Kaohsiung Medical University, Kaohsiung 807, Taiwan ; National Sun Yat-sen University-Kaohsiung Medical University Joint Research Center, Kaohsiung 807, Taiwan ; Center of Biomedical Engineering and System Biology, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
Yu-Chang
0000-0002-1917-5893
YC
Tyan
D049109:Cell Proliferation / Q000187:drug effects*; D048271:Chitosan / Q000737:chemistry / Q000494:pharmacology*; D056945:Hep G2 Cells; D006801:Humans; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008113:Liver Neoplasms / Q000378:metabolism* / Q000473:pathology; D053758:Nanoparticles; D009362:Neoplasm Metastasis; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects*; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism*
D048271:Chitosan; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D058570:TOR Serine-Threonine Kinases; C546842:MTOR protein, human; C494918:AKT1 protein, human