Monocyte-based microrobot with chemotactic motility for tumor theragnosis.
Biocompatibility, sensing, and self-actuation are very important features for a therapeutic biomedical microrobot. As a new concept for tumor theragnosis, this paper proposes a monocyte-based microrobots, which are combining the phagocytosis and engulfment activities containing human acute monocytic leukemia cell line (THP-1) with various sized polystyrene microbeads are engulfed instead of a therapeutic drug. For the validation of the blood vessel barrier-penetrating activity of the monocyte-based microrobot, we fabricate a new cell migration assay with monolayer-cultured endothelial cell (HUVEC), similar with the blood vessels. We perform the penetrating chemotactic motility of the monocyte-based microrobot using various types of the chemo-attractants, such as monocyte chemotactic protein (MCP)-1, human breast cancer cell lines (MCF7)-cell lysates, and -contained alginate spheroids. The monocyte-based microrobot show chemotactic transmigrating motilities similar with what an actual monocyte does. This new paradigm of a monocyte-based microrobot having various useful properties such as biocompatibility, sensing, and self-actuation can become the basis of a biomedical microrobot using monocytes for diagnosis and therapy of various diseases.
School of Mechanical Engineering, Chonnam National University, Gwangju, 500-757, Republic of Korea.
Sung Jun
SJ
Park
Yeonkyung
Y
Lee
Young Jin
YJ
Choi
Sunghoon
S
Cho
Han-Earl
HE
Jung
Shaohui
S
Zheng
Bang Ju
BJ
Park
Seong Young
SY
Ko
Jong-Oh
JO
Park
Sukho
S
Park
D045744:Cell Line, Tumor; D018932:Chemokine CCL2 / Q000276:immunology*; D002633:Chemotaxis; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D061986:MCF-7 Cells; D009000:Monocytes / Q000166:cytology* / Q000276:immunology; D009369:Neoplasms / Q000175:diagnosis / Q000628:therapy; D010587:Phagocytosis
D018932:Chemokine CCL2
chemotaxis; microrobot; monocyte; monocyte chemotactic protein; transmigration; tumor theragnosis