Lipoteichoic acid from Lactobacillus plantarum inhibits the expression of platelet-activating factor receptor induced by Staphylococcus aureus lipoteichoic acid or Escherichia coli lipopolysaccharide in human monocyte-like cells.
Platelet-activating factor receptor (PAFR) plays an important role in bacterial infection and inflammation. We examined the effect of the bacterial cell wall components lipopolysaccharide (LPS) and lipoteichoic acid (LTA) from Lactobacillus plantarum (pLTA) and Staphylococcus aureus (aLTA) on PAFR expression in THP-1, a monocyte-like cell line. LPS and aLTA, but not pLTA, significantly increased PAFR expression, whereas priming with pLTA inhibited LPSmediated or aLTA-mediated PAFR expression. Expression of Toll-like receptor (TLR) 2 and 4, and CD14 increased with LPS and aLTA treatments, but was inhibited by pLTA pretreatment. Neutralizing antibodies against TLR2, TLR4, and CD14 showed that these receptors were important in LPS-mediated or aLTA-mediated PAFR expression. PAFR expression is mainly regulated by the nuclear factor kappa B signaling pathway. Blocking PAF binding to PAFR using a PAFR inhibitor indicated that LPS-mediated or aLTA-mediated PAF expression affected TNF-α production. In the mouse small intestine, pLTA inhibited PAFR, TLR2, and TLR4 expression that was induced by heat-labile toxin. Our data suggested that pLTA has an anti-inflammatory effect by inhibiting the expression of PAFR that was induced by pathogenic ligands.
School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University, Yongin 449-701, Republic of Korea.
Hangeun
H
Kim
Bong Jun
BJ
Jung
Jihye
J
Jeong
Honam
H
Chun
Dae Kyun
DK
Chung
D002460:Cell Line; D004926:Escherichia coli / Q000276:immunology*; D020869:Gene Expression Profiling; D006801:Humans; D007155:Immunologic Factors / Q000378:metabolism*; D048191:Lactiplantibacillus plantarum / Q000276:immunology*; D008070:Lipopolysaccharides / Q000378:metabolism*; D009000:Monocytes / Q000276:immunology / Q000382:microbiology; D010980:Platelet Membrane Glycoproteins / Q000037:antagonists & inhibitors*; D043562:Receptors, G-Protein-Coupled / Q000037:antagonists & inhibitors*; D013211:Staphylococcus aureus / Q000276:immunology*; D013682:Teichoic Acids / Q000378:metabolism*
D007155:Immunologic Factors; D008070:Lipopolysaccharides; D010980:Platelet Membrane Glycoproteins; D043562:Receptors, G-Protein-Coupled; D013682:Teichoic Acids; C039351:platelet activating factor receptor; C009900:lipoteichoic acid