High-level coproduction, purification and characterisation of laccase and exopolysaccharides by Coriolus versicolor.
In this study, a two-stage pH-shift fermentation process was developed for the coproduction of laccase and exopolysaccharides (EPS) by Coriolus versicolor. At the same time, laccase and EPS were purified and characterised in detail. The results showed that the highest laccase and EPS production reached 7680 U l(-1) and 8.2 g l(-1). Furthermore, the flow behaviour of fermentation broth was Newtonian and the maximum μ(ap) was 2.7×10(-3) Pa s. The MW of laccase was 64 kDa and it showed a pI value of 4.2. The CD analysis showed that laccase had a high α-helical content (68%). The MW of the purified EPS was determined to be 1.8×10(6) Da, consisting of carbohydrates (87.6%) and proteins (12.4%). The EPS consisted of 17 amino acids, mainly serine (11.3%), glutamic acid (12.60%), leucine (13.3%) and phenylalanine (9.4%) in protein moiety, and three monosaccharides (galactose, mannose and xylose).
Key Lab of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, PR China; College of Crop Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, PR China. Electronic address: queyouxiong@163.com.
Youxiong
Y
Que
Key Lab of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, PR China; College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, PR China; Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou 350002, PR China. Electronic address: shjsun2004@126.com.
Shujing
S
Sun
Key Lab of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, PR China; College of Crop Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, PR China. Electronic address: xlpmail@126.com.
Liping
L
Xu
Key Lab of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, PR China; College of Crop Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, PR China.
Yuye
Y
Zhang
College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, PR China; Centre for Bioengineering and Biotechnology, China University of Petroleum (East China), Qingdao 266580, PR China. Electronic address: zhuhufafu@gmail.com.
Hu
H
Zhu
D005285:Fermentation; D062610:Fungal Polysaccharides / Q000096:biosynthesis* / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D042845:Laccase / Q000096:biosynthesis* / Q000302:isolation & purification; D055454:Trametes / Q000378:metabolism*
D062610:Fungal Polysaccharides; D042845:Laccase
Coriolus versicolor; Exopolysaccharides; Laccase; Metabolite purification and accumulation; pH controlled fermentation