Correlation of cytohistlogical expression and serum level of ca125 in ovarian neoplasm.
Context or Background: CA125 is a biomarker that has potential utility across the spectrum: risk assessment, early detection, diagnosis, prognosis, monitoring and therapy.


OBJECTIVE
This study was conducted to establish the validity and reliability of correlation of CA125 serum level with immunochemistry expression in imprint cytology and tissues for diagnostic purpose.


METHODS
A prospective study was done on 50 cases of clinically and radiologically diagnosed ovarian tumor. Imprint smears were made intraoperatively from fresh samples and stained with M.G.G. stain for air dried smears and Papanicoloau stain for alcohol fixed smears. Stained smear was assessed and compared with subsequent histopathology report. Preoperative blood samples were obtained from all patients and sent for the assay of serum CA125 levels. Analysis of CA125 immunochemistry expression in imprint cytology and tissue was done and correlated with preoperative serum blood CA125 levels.


RESULTS
Significant positive correlation was found between elevated serum CA125 levels and cytohistological expression of CA125. Overall sensitivity was 100%, specificity was 86%, positive predictive value was 74% and negative predictive value 100%. Diagnostic accuracy was 90% with high statistical significance (p<0.001).


CONCLUSIONS
We considered 35 U/mL as the cut-off value when evaluating serum CA125 ovarian cancer. Patients with high serum levels show good cytohistological expression.
PGT, Department of Pathology, Institute of Post-Graduate Medical Education and Research , West Bengal, Kolkata, India .
Chhanda
C
Das
Professor, Department of Pathology, Institute of Post-Graduate Medical Education and Research , West Bengal, India .
Madhumita
M
Mukhopadhyay
Professor, Department of Gynaecology and Obstetrics, Institute of Post-Graduate Medical Education and Research , West Bengal, Kolkata, India .
Tarun
T
Ghosh
Assistant Professor, Department of Surgery, Bankura Sammilani Medical College , Bankura, India .
Ashis Kumar
AK
Saha
Demonstrator, Department of Pathology, Institute of Post-Graduate Medical Education and Research , West Bengal, Kolkata, India .
Moumita
M
Sengupta
CA125; Imprint cytology; Ovarian tumor