Sub-diffraction resolution pump-probe microscopy with shot-noise limited sensitivity using laser diodes.
We demonstrate the use of intensity-modulated laser diodes to implement pump-probe microscopy and achieved sub-diffraction resolution imaging with shot-noise limited sensitivity with a scheme of balanced detection. This technique has several applications for various types of induced transmission change, including excited-state absorption, ground state absorption bleaching and stimulated emission. By using our technique, biological imaging of mouse T cells and the axons of neurons in the cerebral cortex was demonstrated.
Jun
J
Miyazaki
Hiromichi
H
Tsurui
Akiko
A
Hayashi-Takagi
Haruo
H
Kasai
Takayoshi
T
Kobayashi
D000818:Animals; D054023:Lasers, Semiconductor; D051379:Mice; D008853:Microscopy / Q000295:instrumentation*; D009622:Noise