A bi-cistronic, reporter-encoding bovine viral diarrhea virus applied in a new, effective diagnostic test.
Infections with bovine viral diarrhea virus (BVDV) have a huge economic impact on cattle production and reproduction worldwide. A key factor for BVDV surveillance and eventual eradication is to efficiently detect infections and to monitor herd immunity. In this study, we generated a stable, bi-cistronic BVDV that encoded EGFP in addition to the viral proteins. Applying this recombinant virus, a new flow-cytometry-based virus neutralization test was established that enabled accurate and reliable detection of field-virus-infected and vaccinated animals. The test, which is simple and fast, is expected to support novel, effective screening procedures in eradication and vaccination programmes.
Institute of Biochemistry and Biotechnology, Faculty of Life Sciences (NFI), Martin Luther University Halle-Wittenberg, Halle, Germany.
Mandy
M
Gebauer
Institute of Biochemistry and Biotechnology, Faculty of Life Sciences (NFI), Martin Luther University Halle-Wittenberg, Halle, Germany.
Martina
M
Behrens
Institue of Virology, Faculty of Veterinary Medicine, Justus-Liebig-University Giessen, Giessen, Germany.
Matthias
M
König
Institute of Biochemistry and Biotechnology, Faculty of Life Sciences (NFI), Martin Luther University Halle-Wittenberg, Halle, Germany.
Sven-Erik
SE
Behrens
D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood*; D000914:Antibodies, Viral / Q000097:blood*; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000175:diagnosis* / Q000276:immunology; D002417:Cattle; D003937:Diagnosis, Differential; D003955:Diagnostic Tests, Routine; D030241:Diarrhea Virus 1, Bovine Viral / Q000235:genetics*; D005434:Flow Cytometry / Q000379:methods; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics; D008403:Mass Screening / Q000379:methods; D009500:Neutralization Tests / Q000379:methods*; D014730:Veterinary Medicine / Q000379:methods*; D014773:Virology / Q000379:methods*
D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins