Enhanced electrochemiluminescence from luminol at carboxyl graphene for detection of α-fetoprotein.
In this study, a novel sensitive electrochemiluminescence (ECL) immunosensor was constructed by carboxyl graphene (GR) for enhancing luminol-O2 system emission. Here, carboxyl GR was used to enhance the ECL intensity of luminol that had excellent electron transfer ability and good solubility. The sensing platform was constructed by depositing carboxyl GR on electrodes and immobilizing antibodies on the surface of carboxyl GR through amidation. The specific immunoreaction between α-fetoprotein (AFP) and antibodies resulted in a decrease of ECL intensity, and the intensity decreased linearly with AFP concentrations in the range of 5 pg ml(-1) to 14 ng ml(-1) with a detection limit of 2.0 pg ml(-1). The proposed immunosensor exhibits high specificity, good reproducibility, and longtime stability. It may become a promising technique for protein detection.
Key Laboratory of Chemical Sensing and Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, People's Republic of China.
Xiaojian
X
Li
College of Resources, Shandong Water Polytechnic, Rizhao 276826, People's Republic of China.
Qingfang
Q
Guo
Key Laboratory of Chemical Sensing and Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, People's Republic of China.
Wei
W
Cao
Key Laboratory of Chemical Sensing and Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, People's Republic of China.
Yueyun
Y
Li
Key Laboratory of Chemical Sensing and Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, People's Republic of China.
Bin
B
Du
Key Laboratory of Chemical Sensing and Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, People's Republic of China. Electronic address: sdjndxwq@163.com.
Qin
Q
Wei
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology; D014408:Biomarkers, Tumor / Q000032:analysis; D015374:Biosensing Techniques / Q000379:methods; D002417:Cattle; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry / Q000276:immunology; D049449:Luminescence; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods; D008165:Luminol / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine; D000509:alpha-Fetoproteins / Q000737:chemistry*
D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D055767:Immobilized Proteins; D000509:alpha-Fetoproteins; D012710:Serum Albumin, Bovine; D008165:Luminol; D006108:Graphite
Carboxyl graphene; Electrochemiluminescence immunosensor; Luminol–O(2) system; α-Fetoprotein