Regulation of human dendritic cell functions by natural anti-CD40 antibodies.
Dendritic cells (DCs) are professional antigen presenting cells that play a pivotal role in the initiation of immune responses. DCs ingest antigens and then present these antigens to T cells to initiate T cell activation and polarization. DCs receive signals both from environment and from endogenous molecules. DCs in the immune system constantly interact with immunoglobulins (or antibodies) and a substantial amount of these immunoglobulins are natural. We found that natural antibodies have a key role in regulating the DC functions and that CD40-reactive natural antibodies constitute one of the endogenous molecules that provide maturation-associated signals to DCs in physiology. In this chapter, we describe the isolation of anti-CD40 natural antibodies from pooled normal immunoglobulin preparations (intravenous immunoglobulin, IVIg) and their biological effects on human DC maturation and functions.
Singapore Immunology Network, Agency for Science Technology and Research (A*STAR), Singapore, Singapore.
Sri Ramulu
SR
Elluru
Srini V
SV
Kaveri
Jagadeesh
J
Bayry
D000911:Antibodies, Monoclonal / Q000097:blood / Q000494:pharmacology*; D000938:Antigen-Presenting Cells / Q000276:immunology*; D019013:CD40 Antigens / Q000276:immunology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology* / Q000187:drug effects / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D016756:Immunoglobulins, Intravenous / Q000276:immunology / Q000378:metabolism*; D009000:Monocytes / Q000166:cytology* / Q000187:drug effects / Q000276:immunology
D000911:Antibodies, Monoclonal; D019013:CD40 Antigens; D016756:Immunoglobulins, Intravenous