Immunolocalization of growth, inhibitory, and proliferative factors involved in initial ovarian folliculogenesis from adult common squirrel monkey (Saimiri collinsi).
We performed an immunohistochemical (IHC) study to determine the follicular expression of growth differentiation factor 9 (GDF-9), anti-Müllerian hormone (AMH), Kit Ligand (KL), and c-Kit in squirrel monkey ovary. Ovarian tissue fragments from 4 squirrel monkeys were collected by laparotomy and processed for classical histology and IHC. Additionally, follicle development was assessed by Ki67 immunostaining to evaluate proliferative status of granulosa cells. A total of 4025 follicles were examined (1475 for classical histology and 2550 for immunohistochemistry). More than 80% of the evaluated follicles were morphologically normal. The GDF-9 protein was detectable in oocyte cytoplasm from primordial (100%), primary (99.1%), and secondary (100%) follicles. The AMH was not expressed in primordial follicles but just in few primary follicles (13.8%). On the other hand, it was highly expressed in granulosa cells from secondary follicles (67.9%). c-Kit, KL receptor, was found in the oolemma of primordial (100%), primary (100%), and secondary (100%) follicles. The KL expression was observed in oocytes and granulosa cells from primordial (94.9%), primary (91.6%) and secondary follicles (100%). Ki67 immunostaining was observed in granulosa cells from primary (5.7%) and secondary (54.8%) follicles but not in primordial follicles. In conclusion, we described the localization of GDF-9, KL, c-Kit, and Ki67 proteins and confirmed the presence of AMH protein in preantral follicles from squirrel monkey. Our results offer contribution for understanding of folliculogenesis in neotropical nonhuman primates. Moreover, these markers can be used to assess follicular viability and functionality after cryopreservation, transplantation, or in vitro culture of ovarian tissue.
Laboratory of Wild Animal Biology and Medicine, Federal University of Pará, Castanhal, Pará, Brazil Animal Sciences PhD Program, Federal University of Pará, Belém, Pará, Brazil National Primate Centre, Secretary of Health Policy, Ministry of Health, Ananindeua, Pará, Brazil sarah.scalercio@cenp.org.br sarahscalercio@gmail.com.
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Scalercio
Laboratory of Wild Animal Biology and Medicine, Federal University of Pará, Castanhal, Pará, Brazil Animal Sciences PhD Program, Federal University of Pará, Belém, Pará, Brazil.
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Brito
Laboratory of Wild Animal Biology and Medicine, Federal University of Pará, Castanhal, Pará, Brazil Animal Sciences PhD Program, Federal University of Pará, Belém, Pará, Brazil.
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Domingues
Laboratory of Wild Animal Biology and Medicine, Federal University of Pará, Castanhal, Pará, Brazil Animal Sciences PhD Program, Federal University of Pará, Belém, Pará, Brazil Faculty of Veterinary Medicine, Utrecht University, Utrecht, the Netherlands.
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Santos
Pôle de Recherche en Gynécologie, Institut de Recherche Expérimentale et Clinique, Université Catholique de Louvain, Brussels, Belgium.
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Amorim
D000367:Age Factors; D000818:Animals; D054304:Anti-Mullerian Hormone / Q000032:analysis*; D049109:Cell Proliferation; D005260:Female; D055429:Growth Differentiation Factor 9 / Q000032:analysis*; D007150:Immunohistochemistry; D006080:Ovarian Follicle / Q000737:chemistry* / Q000166:cytology; D019009:Proto-Oncogene Proteins c-kit / Q000032:analysis*; D012453:Saimiri / Q000378:metabolism / Q000502:physiology*; D019089:Stem Cell Factor / Q000037:antagonists & inhibitors*
D055429:Growth Differentiation Factor 9; D019089:Stem Cell Factor; D054304:Anti-Mullerian Hormone; D019009:Proto-Oncogene Proteins c-kit
cell proliferation; immunohistochemistry; neotropical primate; ovarian tissue; preantral follicles