Metabolic Evidence of Diminished Lipid Oxidation in Women With Polycystic Ovary Syndrome.
Polycystic ovary syndrome (PCOS), a common female endocrinopathy, is a complex metabolic syndrome of enhanced weight gain. The goal of this pilot study was to evaluate metabolic differences between normal (n=10) and PCOS (n=10) women via breath carbon isotope ratio, urinary nitrogen and nuclear magnetic resonance (NMR)-determined serum metabolites. Breath carbon stable isotopes measured by cavity ring down spectroscopy (CRDS) indicated diminished (p<0.030) lipid use as a metabolic substrate during overnight fasting in PCOS compared to normal women. Accompanying urinary analyses showed a trending correlation (p<0.057) between overnight total nitrogen and circulating testosterone in PCOS women, alone. Serum analyzed by NMR spectroscopy following overnight, fast and at 2 h following an oral glucose tolerance test showed that a transient elevation in blood glucose levels decreased circulating levels of lipid, glucose and amino acid metabolic intermediates (acetone, 2-oxocaporate, 2-aminobutyrate, pyruvate, formate, and sarcosine) in PCOS women, whereas the 2 h glucose challenge led to increases in the same intermediates in normal women. These pilot data suggest that PCOS-related inflexibility in fasting-related switching between lipid and carbohydrate/protein utilization for carbon metabolism may contribute to enhanced weight gain.
Paso del Norte Institute for Healthy Living, 500 W. University Ave, El Paso, TX 79968, USA.
Leah D
LD
Whigham
Animal Sciences Department, UW-Madison, 1675 Observatory Drive, Madison, WI 53706, USA.
Daniel E
DE
Butz
National Magnetic Resonance Facility at Madison, UW-Madison, 433 Babcock Drive, Madison WI 53706, USA.
Hesam
H
Dashti
National Magnetic Resonance Facility at Madison, UW-Madison, 433 Babcock Drive, Madison WI 53706, USA.
Marco
M
Tonelli
Paso del Norte Institute for Healthy Living, 500 W. University Ave, El Paso, TX 79968, USA.
Luann K
LK
Johnson
Animal Sciences Department, UW-Madison, 1675 Observatory Drive, Madison, WI 53706, USA.
Mark E
ME
Cook
Department of Zoology, UW-Madison, 1117 W. Johnson St. Madison, WI 53706, USA.
Warren P
WP
Porter
Department of Molecular and Cellular Physiology, University of Cincinnati, 231 Albert B. Sabin Way, Cincinnati, OH 45267-0576, USA.
Hamid R
HR
Eghbalnia
National Magnetic Resonance Facility at Madison, UW-Madison, 433 Babcock Drive, Madison WI 53706, USA ; Department of Biochemistry, UW-Madison, 433 Babcock Drive, Madison WI 53706, USA.
John L
JL
Markley
Arizona Reproductive Institute 1775 E Skyline Drive, Tucson, AZ 85718, USA.
Steven R
SR
Lindheim
Department of Nutritional Sciences, UW-Madison, 1415 Linden Drive, Madison, WI 53706, USA.
Dale A
DA
Schoeller
Department of Obstetrics and Gynecology and Wisconsin National Primate Research Center, UW-Madison, 1223 Capitol Court, Madison, WI 53715, USA.
David H
DH
Abbott
National Magnetic Resonance Facility at Madison, UW-Madison, 433 Babcock Drive, Madison WI 53706, USA ; Department of Biochemistry, UW-Madison, 433 Babcock Drive, Madison WI 53706, USA ; Department of Nutritional and Human Health Sciences, Texas Tech University, 1301 Akron Ave, Lubbock, TX, 79409, USA.
Fariba M
FM
Assadi-Porter
Cavity ring down; NMR-metabolomics; glucose elevation; lipid metabolism; polycystic ovary syndrome (PCOS)