Cytogenetic analysis of oral mucosa cells, induced by chlorhexidine, essential oils in ethanolic solution and triclosan mouthwashes.
OBJECTIVE
The aim of this study was to evaluate DNA damage and cytokinetic defects, proliferative potential and cell death caused by the frequent use of mouthrinses containing chlorhexidine, triclosan and essential oils in ethanolic solution, compared to a placebo mouthwash.


METHODS
This double-blind, prospective, randomized clinical trial included 80 Caucasian patients. Subjects were divided into four groups: Group I used a mouthrinse, Triclosan; Group II used physiological saline; Group III used chlorhexidine; Group IV a mouthrinse with essential oils in ethanolic solution. All subjects used the mouthrinses for two weeks (15 ml, twice a day, rinsing for 30s). Two cell samples per subject were collected, before and after mouthrinse use (on day 0 and day 15). Samples were processed as follows: cell collection from cheeks with a cytobrush; cell centrifuge; slide preparation, fixation and staining; and fluorescent microscope analysis. 2000 exfoliated cells were screened for nuclear abnormalities, particularly the presence of micronuclei by means of cytome assay.


RESULTS
No significant differences between study times (before and after use of mouthwash) were identified for any of the variables studied (p>0.05). Differences between mouthrinse groups were also compared but no significant differences were found (p>0.05).


CONCLUSIONS
This study did not observe any genotoxic effect resulting from mouthrinse use.
Department of Oral Medicine, Faculty of Medicine and Dentistry. Ageing Research Institute, University of Murcia, Murcia, Spain.
Irene
I
Ros-Llor
Department of Oral Medicine, Faculty of Medicine and Dentistry. Ageing Research Institute, University of Murcia, Murcia, Spain. Electronic address: majornet@um.es.
Pia
P
Lopez-Jornet
D000293:Adolescent; D000328:Adult; D000368:Aged; D000891:Anti-Infective Agents, Local / Q000008:administration & dosage / Q000009:adverse effects; D002455:Cell Division / Q000187:drug effects; D002710:Chlorhexidine / Q000008:administration & dosage / Q000009:adverse effects*; D020732:Cytogenetic Analysis; D004249:DNA Damage; D004311:Double-Blind Method; D000431:Ethanol / Q000008:administration & dosage / Q000009:adverse effects; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009061:Mouth Mucosa / Q000187:drug effects*; D009067:Mouthwashes / Q000009:adverse effects*; D009822:Oils, Volatile / Q000008:administration & dosage / Q000009:adverse effects; D011446:Prospective Studies; D014260:Triclosan / Q000008:administration & dosage / Q000009:adverse effects*; D055815:Young Adult
D000891:Anti-Infective Agents, Local; D009067:Mouthwashes; D009822:Oils, Volatile; D000431:Ethanol; D014260:Triclosan; D002710:Chlorhexidine
Buccal exfoliated cells; Cytome assay; Mouthwash