Applying carbon dioxide, plant growth-promoting rhizobacterium and EDTA can enhance the phytoremediation efficiency of ryegrass in a soil polluted with zinc, arsenic, cadmium and lead.
This study was conducted to investigate the use of elevated carbon dioxide (CO2), plant growth-promoting rhizobacterium Burkholderia sp. D54 (PGPR) and ethylenediaminetetraacetic acid (EDTA) to enhance the phytoextraction efficiency of ryegrass in response to multiple heavy metal (or metalloid)-polluted soil containing zinc (Zn), arsenic (As), cadmium (Cd) and lead (Pb). All of the single or combined CO2, PGPR and EDTA treatments promoted ryegrass growth. The stimulation of ryegrass growth by CO2 and PGPR could primarily be attributed to the regulation of photosynthesis rather than decreased levels of Zn, As and Cd in the shoots. Most treatments seemed to reduce the Zn, As and Cd contents in the shoots, which might be associated with enhanced shoot biomass, thus causing a "dilution effect" regarding their levels. The combined treatments seemed to perform better than single treatments in removing Zn, As, Cd and Pb from soil, judging from the larger biomass and relatively higher total amounts (TAs) of Zn, As, Cd and Pb in both the shoots and roots. Therefore, we suggest that the CO2 plus PGPR treatment will be suitable for removing Zn, As, Cd and Pb from heavy metal (or metalloid)-polluted soils using ryegrass as a phytoremediation material.
Institute of Agro-Environmental Protection, The Ministry of Agriculture, Tianjin 300191, China.
Junkang
J
Guo
Institute of Agro-Environmental Protection, The Ministry of Agriculture, Tianjin 300191, China. Electronic address: frwzym@hotmail.com.
Renwei
R
Feng
Institute of Agro-Environmental Protection, The Ministry of Agriculture, Tianjin 300191, China.
Yongzhen
Y
Ding
Institute of Agro-Environmental Protection, The Ministry of Agriculture, Tianjin 300191, China. Electronic address: 3761520835@sina.com.cn.
Ruigang
R
Wang
D001151:Arsenic / Q000378:metabolism; D001673:Biodegradation, Environmental; D018533:Biomass; D019117:Burkholderia; D002245:Carbon Dioxide / Q000494:pharmacology*; D002614:Chelating Agents / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology*; D008129:Lolium / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism* / Q000382:microbiology; D019216:Metals, Heavy / Q000378:metabolism; D063245:Plant Development; D018517:Plant Roots / Q000737:chemistry; D027343:Rhizome / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism / Q000382:microbiology*; D012989:Soil Pollutants / Q000378:metabolism*
D002614:Chelating Agents; D019216:Metals, Heavy; D012989:Soil Pollutants; D002245:Carbon Dioxide; D004492:Edetic Acid; D001151:Arsenic
Multiple heavy metal contamination; PGPR; Photosynthesis; Phytoextraction; Pigments