Oryzihumus terrae sp. nov., isolated from soil and emended description of the genus Oryzihumus.
A Gram-stain-positive, aerobic, non-flagellated bacterium, designated KIS22-12(T), was isolated from a soil sample of Baengnyeong Island in Onjin county, Republic of Korea. Cells were non-spore-forming cocci showing catalase-positive and oxidase-negative reactions. Growth of strain KIS22-12(T) was observed between 10 and 35 °C (optimum, 28-30 °C), between pH 5.0 and 9.0 (optimum, pH 7.0) and with 0-3% (w/v) NaCl. Strain KIS22-12(T) contained MK-8(H4) as the predominant menaquinone, and C17 : 1ω8c, iso-C15 : 0 and anteiso-C15 : 0 as the major fatty acids. Strain KIS22-12(T) contained diphosphatidylglycerol, phosphatidylinositol, one unknown aminophospholipid, one unknown aminolipid, two unknown phospholipids and one unknown lipid. The peptidoglycan type was A1γ. The G+C content of the genomic DNA was 75.0 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain KIS22-12(T) formed a phyletic lineage with Oryzihumus leptocrescens KV-628(T). 16S rRNA gene sequence similarity between the two strains was 96.5%. On the basis of phenotypic, chemotaxonomic and molecular properties, strain KIS22-12(T) represents a novel species within the genus Oryzihumus, for which the name Oryzihumus terrae sp. nov. is proposed. The type strain is KIS22-12(T) ( = KACC 16543(T) = DSM 27161(T) = NBRC 109596(T)). An emended description of the genus Oryzihumus is also provided.
Department of Microbiology, Dankook University, Anseo-dong, Cheonan 330-714, Republic of Korea.
Jun-Muk
JM
Lim
Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration, Suwon, Republic of Korea.
Soo-Jin
SJ
Kim
NITE Biological Resource Center (NBRC), National Institute of Technology and Evaluation, 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan.
Moriyuki
M
Hamada
Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration, Suwon, Republic of Korea.
Jae-Hyung
JH
Ahn
Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration, Suwon, Republic of Korea.
Hang-Yeon
HY
Weon
NITE Biological Resource Center (NBRC), National Institute of Technology and Evaluation, 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan.
Ken-Ichiro
KI
Suzuki
Department of Microbiology, Dankook University, Anseo-dong, Cheonan 330-714, Republic of Korea.
Tae-Young
TY
Ahn
Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration, Suwon, Republic of Korea.
Soon-Wo
SW
Kwon
D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D010457:Peptidoglycan / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C024223:vitamin MK 8