Uniform fluorescent nanobioprobes for pathogen detection.
Manipulating biochemical reactions in living cells to synthesize nanomaterials is an attractive strategy to realize their synthesis that cannot take place in nature. Yeast cells have been skillfully utilized to produce desired nanoparticles through spatiotemporal coupling of intracellular nonrelated biochemical reaction pathways for formation of fluorescent CdSe quantum dots. Here, we have successfully transformed Staphylococcus aureus cells into cellular beacons (fluorescing cells), all of which are highly fluorescent and photostable with perfect uniformity. Importantly, on the basis of such cells, we efficiently fabricated fluorescent nanobioprobes by a specific interaction between the protein A expressed on the S. aureus surface and the Fc fragment domain of antibodies, avoiding the use of other common methods for cell surface modifications, such as molecular covalent connection or more difficult genetic and metabolic engineering. Coupled with immunomagnetic beads, the resulting fluorescent-biotargeting bifunctional cells, i.e., biotargeting cellular beacons, can be employed as nanobioprobes for detection of viruses, bacteria, and tumor cells. With this method, H9N2 AIV can be detected specifically with a limit of 8.94 ng/mL (based on protein content). Furthermore, diverse probes for detection of different pathogens or for other biomedical applications can be easily obtained by simply changing the antibody conjugated to the cell surface.
Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Science, State Key Laboratory of Virology, and Wuhan Institute of Biotechnology, Wuhan University , Wuhan 430072, People's Republic of China.
Ling-Hong
LH
Xiong
Ran
R
Cui
Zhi-Ling
ZL
Zhang
Xu
X
Yu
Zhixiong
Z
Xie
Yun-Bo
YB
Shi
Dai-Wen
DW
Pang
D015374:Biosensing Techniques; D045744:Cell Line, Tumor; D004867:Equipment Design; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D007141:Immunoglobulin Fc Fragments / Q000737:chemistry; D018189:Immunomagnetic Separation; D053127:Influenza A Virus, H9N2 Subtype; D008024:Ligands; D057230:Limit of Detection; D008422:Materials Testing; D060847:Metabolic Engineering; D008854:Microscopy, Electron; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D053758:Nanoparticles / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods*; D055095:Optics and Photonics; D045663:Quantum Dots; D012643:Selenium / Q000737:chemistry*; D013211:Staphylococcus aureus
D005456:Fluorescent Dyes; D007141:Immunoglobulin Fc Fragments; D008024:Ligands; D012643:Selenium