The effects of trimetazidine and sildenafil on bilateral cavernosal nerve injury induced oxidative damage and cavernosal fibrosis in rats.
OBJECTIVE
The aim of this study was to compare the effects of sildenafil and trimetazidine on bilateral cavernosal nerve injury-induced oxidative damage and fibrotic changes in cavernosal tissue in rat model.


METHODS
A total of 32 male Sprague-Dawley rats were randomly divided into 4 groups; each group consist 8 rats (control, BCI, BCI + TMZ, and BCI + sildenafil groups). Tissue superoxide dismutase (SOD), malondialdehyde (MDA), and protein carbonyl (PC) levels were determined biochemically and distribution of cavernosal fibrosis density among groups was performed histopathologically.


RESULTS
Tissue SOD levels in BCI group were significantly lower than the control group (P < 0.05). Tissue MDA and PC levels in BCI group were significantly higher than the control group (P < 0.05). TMZ and sildenafil administration significantly increased tissue SOD levels (P < 0.05) and reduced tissue MDA and PC levels (P < 0.05). Histologically, the degree of cavernosal fibrosis and collagen density was higher in BCI group in comparison to control, TMZ-treated, and sildenafil-treated groups.


CONCLUSIONS
BCI caused oxidative damage and increased cavernosal fibrosis in rat penis. TMZ and sildenafil treatment decreased oxidative damage and reduced the degree of fibrosis in penile tissue due to BCI.
Department of Urology, Medical Faculty, Gaziosmanpasa University, 60100 Tokat, Turkey.
Dogan
0000-0001-8584-2124
D
Atilgan
Department of Urology, Medical Faculty, Gaziosmanpasa University, 60100 Tokat, Turkey.
Bekir S
BS
Parlaktas
Department of Urology, Medical Faculty, Gaziosmanpasa University, 60100 Tokat, Turkey.
Nihat
N
Uluocak
Department of Urology, Medical Faculty, Gaziosmanpasa University, 60100 Tokat, Turkey.
Fikret
F
Erdemir
Department of Pathology, Medical Faculty, Gaziosmanpasa University, 60100 Tokat, Turkey.
Fatma
F
Markoc
Department of Biochemistry, Medical Faculty, Gaziosmanpasa University, 60100 Tokat, Turkey.
Oguzhan
O
Saylan
Department of Biostatistics, Medical Faculty, Gaziosmanpasa University, 60100 Tokat, Turkey.
Unal
U
Erkorkmaz
D000818:Animals; D005355:Fibrosis / Q000378:metabolism / Q000503:physiopathology / Q000517:prevention & control*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D010413:Penis / Q000187:drug effects* / Q000294:innervation / Q000473:pathology; D010879:Piperazines / Q000494:pharmacology*; D011687:Purines / Q000494:pharmacology; D011897:Random Allocation; D017207:Rats, Sprague-Dawley; D000068677:Sildenafil Citrate; D013450:Sulfones / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism; D020196:Trauma, Nervous System / Q000378:metabolism / Q000503:physiopathology / Q000517:prevention & control*; D016896:Treatment Outcome; D014292:Trimetazidine / Q000494:pharmacology*; D014665:Vasodilator Agents / Q000494:pharmacology
D010879:Piperazines; D011687:Purines; D013450:Sulfones; D014665:Vasodilator Agents; D008315:Malondialdehyde; D000068677:Sildenafil Citrate; D013482:Superoxide Dismutase; D014292:Trimetazidine