Immunostimulatory effects of polysaccharides isolated from Makgeolli (traditional Korean rice wine).
Makgeolli is a traditional Korean rice wine, reported to have various biological functions. In this study, the immunostimulatory activity of a polysaccharide from makgeolli (PSM) was investigated. The polysaccharide fraction was isolated from makgeolli by hot water extraction, ethanol precipitation, dialysis, and lyophilization. The major constituents in PSM were neutral sugars (87.3%). PSM was composed of five different sugars, glucose, mannose, galactose, xylose, and arabinose. In normal mice, PSM treatment increased the spleen index (p<0.05) as well as splenocyte proliferation (p<0.05) in combination with concanavalin A or lipopolysaccharide. The immunostimulatory activities of PSM were also examined in cyclophosphamide (CY)-induced immunosuppressed mice. Mice treated with PSM exhibited increased splenocyte proliferation (p<0.05), natural killer cell activity, and white blood cell counts (p<0.01) compared with immunosuppressed mice. These results indicate that PSM can enhance immune function in normal mice and CY-induced immunosuppressed mice.
Division of Convergence Technology, Korea Food Research Institute, Seongnam 463-746, Korea. cwcho@kfri.re.kr.
Chang-Won
CW
Cho
Department of Preventive Medicine, Yanbian University, Yanji 133000, China. hanchji@hotmail.com.
Chun-ji
CJ
Han
Division of Convergence Technology, Korea Food Research Institute, Seongnam 463-746, Korea. ykrhee@kfri.re.kr.
Young Kyoung
YK
Rhee
Division of Convergence Technology, Korea Food Research Institute, Seongnam 463-746, Korea. yclee@kfri.re.kr.
Young-Chul
YC
Lee
Department of Food Science and Biotechnology, Kyonggi University, Suwon 443-760, Korea. ksshin@kyonggi.ac.kr.
Kwang-Soon
KS
Shin
Division of Convergence Technology, Korea Food Research Institute, Seongnam 463-746, Korea. honghd@kfri.re.kr.
Hee-Do
HD
Hong
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D003208:Concanavalin A / Q000494:pharmacology; D003520:Cyclophosphamide / Q000494:pharmacology; D005285:Fermentation; D007113:Immunity, Innate / Q000187:drug effects*; D007155:Immunologic Factors / Q000302:isolation & purification / Q000494:pharmacology*; D007166:Immunosuppressive Agents / Q000494:pharmacology; D007694:Killer Cells, Natural / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D008070:Lipopolysaccharides / Q000494:pharmacology; D059625:Liquid-Liquid Extraction; D008297:Male; D051379:Mice; D012275:Oryza / Q000737:chemistry; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D014920:Wine / Q000032:analysis*
D007155:Immunologic Factors; D007166:Immunosuppressive Agents; D008070:Lipopolysaccharides; D011134:Polysaccharides; D003208:Concanavalin A; D003520:Cyclophosphamide