Comparative study of total hydroperoxides and antioxidant defense system in the Indian tropical tasar silkworm, Antheraea mylitta, in diapausing and non-diapausing generations.
In the present study, the total hydroperoxides, catalase, glutathione-s-transferase, and ascorbic acid contents were determined in different developmental stages of the non-diapause and the diapause generation of the tropical tasar silkworm, Antheraea mylitta Drury (Lepidoptera: Saturniidae). The results showed stage-specific significantly higher levels of total hydroperoxides, catalase, and ascorbic acid contents in the non-diapause as compared to the diapause generation (p < 0.05). However, a significantly enhanced level of glutathione-S-transferase activity was observed in mature 5th instar larvae of the diapause generation (p < 0.05). In the case of pupae, significantly higher levels of total hydroperoxides, catalase, and glutathione-s-transferase activity were observed in the non-diapause generation (p < 0.05). These results could be the effect of intensive metabolic transformation that takes place in tissues of the non-diapause generation and causes increased production of reactive oxygen species, such as hydroperoxides. The results suggest that antioxidants play an important role in protecting cells against reactive oxygen species.
Central Tasar Research and Training Institute, Piska Nagri, Ranchi, Jharkhand, India.
Karmabeer
K
Jena
Prasanta K
PK
Kar
Chittithoti S
CS
Babu
Shantakar
S
Giri
Shyam S
SS
Singh
Bhagwan C
BC
Prasad
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D064666:Diapause, Insect / Q000502:physiology*; D006861:Hydrogen Peroxide / Q000378:metabolism*; D007814:Larva; D009036:Moths / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D011679:Pupa; D013053:Spectrophotometry
D000975:Antioxidants; D006861:Hydrogen Peroxide