Mitochondrial genome interrogation for forensic casework and research studies.
This unit describes methods used in the analysis of mitochondrial DNA (mtDNA) for forensic and research applications. UNIT describes procedures specifically for forensic casework where the DNA from evidentiary material is often degraded or inhibited. In this unit, protocols are described for quantification of mtDNA before amplification; amplification of the entire control region from high-quality samples as well as procedures for interrogating the whole mitochondrial genome (mtGenome); quantification of mtDNA post-amplification; and, post-PCR cleanup and sequencing. The protocols for amplification were developed for high-throughput databasing applications for forensic DNA testing such as reference samples and population studies. However, these same protocols can be applied to biomedical research such as age-related disease and health disparities research.
Department of Molecular & Medical Genetics, UNT Health Science Center, Fort Worth, Texas.|Institute of Applied Genetics, UNT Health Science Center, Fort Worth, Texas.
Rhonda K
RK
Roby
Department of Molecular & Medical Genetics, UNT Health Science Center, Fort Worth, Texas.
Marc
M
Sprouse
Department of Molecular & Medical Genetics, UNT Health Science Center, Fort Worth, Texas.
Nicole
N
Phillips
Department of Molecular & Medical Genetics, UNT Health Science Center, Fort Worth, Texas.
Alessandra
A
Alicea-Centeno
Department of Molecular & Medical Genetics, UNT Health Science Center, Fort Worth, Texas.
Shantanu
S
Shewale
TriLink BioTechnologies, Inc, San Diego, California.
Sabrina
S
Shore
TriLink BioTechnologies, Inc, San Diego, California.
Natasha
N
Paul
D004272:DNA, Mitochondrial / Q000235:genetics*; D053803:Forensic Genetics; D054629:Genome, Mitochondrial; D006801:Humans; D060888:Real-Time Polymerase Chain Reaction
D004272:DNA, Mitochondrial
PCR; amplification; forensic; mtDNA; mtGenome; quantification; sequencing