Periodontal tissue regeneration by transplantation of rat adipose-derived stromal cells in combination with PLGA-based solid scaffolds.
Regeneration of damaged periodontium is challenging due to its multi-tissue composition. Mesenchymalstem cell-based approaches using adipose-derived stromal cells (ASCs) may contribute to periodontal reconstruction, particularly when combined with the use of scaffolds to maintain a space for new tissue growth. The aim of this study was to assess the regenerative potential of ASCs derived from inbred or outbred rats in combination with novel solid scaffolds composed of PLGA (Poly D,L-lactic-co-glycolic acid) (PLGA-scaffolds). Cultured ASCs seeded onto PLGA scaffolds (ASCs/PLGA) or PLGA-scaffolds (PLGA) alone were transplanted into periodontal fenestration defects created in F344 or Sprague Dawley (SD) rats. Micro-CT analysis showed a significantly higher percentage of bone growth in the ASCs/PLGA groups compared with the PLGA-alone groups at five weeks after surgery. Similarly, histomorphometric analysis demonstrated thicker growth of periodontal ligament and cementum layers in the ASCs/PLGA-groups compared with the PLGA-alone groups. In addition, transplanted DiI-labeled ASCs were observed in the periodontal regenerative sites. The present investigation demonstrated the marked ability of ASCs in combination with PLGA scaffolds to repair periodontal defects.
Nihon University Graduate School of Dentistry, 1-8-13 Kanda-Surugadai, Chiyoda-ku Tokyo 101-8310, Japan.
Daisuke
D
Akita
Masakazu
M
Morokuma
Yoko
Y
Saito
Katsuyuki
K
Yamanaka
Yuko
Y
Akiyama
Momoko
M
Sato
Takayuki
T
Mashimo
Taku
T
Toriumi
Yoshinori
Y
Arai
Tadashi
T
Kaneko
Naoki
N
Tsukimura
Keitaro
K
Isokawa
Tomohiko
T
Ishigami
Masaki J
MJ
Honda
D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D003739:Dental Cementum; D019344:Lactic Acid; D008297:Male; D010513:Periodontal Ligament; D010519:Periodontium / Q000000981:diagnostic imaging / Q000502:physiology*; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D051381:Rats; D012038:Regeneration; D017154:Stromal Cells / Q000637:transplantation*; D054457:Tissue Scaffolds; D014945:Wound Healing; D055114:X-Ray Microtomography
D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid