Characterization of Staphylococcus aureus from distinct geographic locations in China: an increasing prevalence of spa-t030 and SCCmec type III.
Staphylococcus aureus belongs to one of the most common bacteria causing healthcare and community associated infections in China, but their molecular characterization has not been well studied. From May 2011 to June 2012, a total of 322 non-duplicate S. aureus isolates were consecutively collected from seven tertiary care hospitals in seven cities with distinct geographical locations in China, including 171 methicillin sensitive S. aureus (MSSA) and 151 MRSA isolates. All isolates were characterized by spa typing. The presence of virulence genes was tested by PCR. MRSA were further characterized by SCCmec typing. Seventy four and 16 spa types were identified among 168 MSSA and 150 MRSA, respectively. One spa type t030 accounted for 80.1% of all MRSA isolates, which was higher than previously reported, while spa-t037 accounted for only 4.0% of all MRSA isolates. The first six spa types (t309, t189, t034, t377, t078 and t091) accounted for about one third of all MSSA isolates. 121 of 151 MRSA isolates (80.1%) were identified as SCCmec type III. pvl gene was found in 32 MSSA (18.7%) and 5 MRSA (3.3%) isolates, with ST22-MSSA-t309 as the most commonly identified strain. Compared with non-epidemic MRSA clones, epidemic MRSA clones (corresponding to ST239) exhibited a lower susceptibility to rifampin, ciprofloxacin, gentamicin and trimethoprim-sulfamethoxazole, a higher prevalence of sea gene and a lower prevalence of seb, sec, seg, sei and tst genes. The increasing prevalence of multidrug resistant spa-t030 MRSA represents a major public health problem in China.
Center for Hospital Infection Control, Chinese PLA Institute for Disease Control & Prevention, Academy of Military Medical Sciences, Beijing, China.
Yong
Y
Chen
Department of Clinical Microbiology, Urumqi General Hospital of Lanzhou Military Command, PLA, Urumqi, China.
Zhengxiang
Z
Liu
Department of Clinical Microbiology, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
Libo
L
Duo
Department of Clinical Laboratory, General Hospital of Chengdu Military Command, Chengdu, China.
Jie
J
Xiong
Department of Clinical Laboratory, General Hospital of Ji'nan Military Command, Ji'nan, China.
Yanwen
Y
Gong
Department of Clinical Microbiology, Chinese PLA General Hospital, Beijing, China.
Jiyong
J
Yang
Department of Clinical Laboratory, 94 Hospital of PLA, Nanchang, China.
Zhanke
Z
Wang
Department of Clinical Laboratory, The First Affiliated Hospital of Soochow University, Suzhou, China.
Xuqin
X
Wu
Center for Hospital Infection Control, Chinese PLA Institute for Disease Control & Prevention, Academy of Military Medical Sciences, Beijing, China.
Zhongyi
Z
Lu
Department of Clinical Microbiology, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
Xiangzhao
X
Meng
Center for Hospital Infection Control, Chinese PLA Institute for Disease Control & Prevention, Academy of Military Medical Sciences, Beijing, China.
Jingya
J
Zhao
Center for Hospital Infection Control, Chinese PLA Institute for Disease Control & Prevention, Academy of Military Medical Sciences, Beijing, China.
Changjian
C
Zhang
Center for Hospital Infection Control, Chinese PLA Institute for Disease Control & Prevention, Academy of Military Medical Sciences, Beijing, China.
Fang
F
Wang
Center for Hospital Infection Control, Chinese PLA Institute for Disease Control & Prevention, Academy of Military Medical Sciences, Beijing, China.
Yulong
Y
Zhang
Center for Hospital Infection Control, Chinese PLA Institute for Disease Control & Prevention, Academy of Military Medical Sciences, Beijing, China.
Mengqiang
M
Zhang
Center for Hospital Infection Control, Chinese PLA Institute for Disease Control & Prevention, Academy of Military Medical Sciences, Beijing, China.
Li
L
Han
D001426:Bacterial Proteins / Q000235:genetics*; D002681:China / Q000453:epidemiology; D003428:Cross Infection / Q000453:epidemiology / Q000382:microbiology*; D024881:Drug Resistance, Bacterial / Q000235:genetics; D060005:Genotyping Techniques; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity; D013203:Staphylococcal Infections / Q000453:epidemiology / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D014774:Virulence / Q000235:genetics
D001426:Bacterial Proteins