EPR on Flavoproteins.
Flavoproteins often employ radical mechanisms in their enzymatic reactions. This involves paramagnetic species, which can ideally be investigated with electron paramagnetic resonance (EPR) spectroscopy. In this chapter we focus on the example of flavin-based photoreceptors and discuss, how different EPR methods have been used to extract information about the flavin radical's electronic state, its binding pocket, electron-transfer pathways, and about the protein's tertiary and quaternary structure.
Fachbereich Physik, Freie Universität Berlin, Arnimallee 14, Berlin, 14195, Germany, brosi@zedat.fu-berlin.de.
Richard
R
Brosi
Robert
R
Bittl
Christopher
C
Engelhard
D000818:Animals; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods; D005415:Flavins / Q000737:chemistry; D005420:Flavoproteins / Q000737:chemistry* / Q000378:metabolism; D005609:Free Radicals; D008968:Molecular Conformation
D005415:Flavins; D005420:Flavoproteins; D005609:Free Radicals