Aflatoxin M1 contamination in cow and buffalo milk samples from the North West Frontier Province (NWFP) and Punjab provinces of Pakistan.
A total of 178 milk samples (94 of buffalo and 84 of cow) were randomly taken from Punjab and the North West Frontier Province (NWFP) of Pakistan (n = 89 in each province) and analysed for the presence of aflatoxin M1 (AFM1) by HPLC-FLD. From Punjab about 46% of buffalo's and 49% of cow's milks were contaminated with AFM1 as compared with 52% and 51% for milk samples from NWFP, respectively. Overall, the mean AFM1 concentration was 0.046 µg kg(-1) with a maximum of 0.350 µg kg(-1). All samples complied with the Codex Alimentarius limit of 0.50 µg kg(-1) for AFM1 in milk, but 16.3% of samples exceeded the European Union maximum level of 0.05 µg kg(-1). Another set of 415 buffalo's and cow's milk samples (213 morning milks and 202 evening milks) were analysed. Statistical analysis revealed significant differences (p < 0.05) between mean AFM1 concentrations in milk during the morning (0.043 µg kg(-1)) and the evening (0.028 µg kg(-1)) lactation times.
a Department of Applied Chemistry , GC University Faisalabad , 38000, Faisalabad , Pakistan.
S Z
SZ
Iqbal
M R
MR
Asi
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A
Ariño
D016607:Aflatoxin M1 / Q000032:analysis*; D000818:Animals; D002020:Buffaloes; D002417:Cattle; D005506:Food Contamination / Q000032:analysis*; D008892:Milk / Q000737:chemistry*; D010154:Pakistan
D016607:Aflatoxin M1
aflatoxins; milk