Preparation and properties of biocompatible PS-PEG/calcium phosphate nanospheres.
A facile room-temperature method was used to prepare phosphatidylserine (PS)-poly(ethylene glycol) (PEG)/calcium phosphate (CaP) nanospheres (PS-poly(ethylene glycol) methyl ether/CaP nanospheres). Transmission electron microscopy (TEM) results confirmed that the PS-PEG/CaP porous nanospheres were spherical with a diameter of 8-12 nm. X-ray and thermo-gravimetric analysis (TGA) results also confirmed that the PS-PEG micelle was packed in the CaP shell. PS-PEG/CaP nanospheres exhibited little effect on the hemolysis, coagulation characteristics of blood and inflammatory response, demonstrating a negligible cytotoxicity response in LO2 liver cells. Experiments performed in zebrafish demonstrated that the PS-PEG/CaP nanospheres had a long circulatory residence time and did not induce apoptosis in zebrafish. Taken together, these results suggest that the PS-PEG/CaP nanospheres have great potential to be used as a drug carrier.
State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, School of Medicine, Shanghai Jiao Tong University , Shanghai , China and.
Qi
Q
Wang
Peifeng
P
Liu
Ying
Y
Sun
Tao
T
Gong
Mingjie
M
Zhu
Xun
X
Sun
Zhirong
Z
Zhang
Yourong
Y
Duan
D000818:Animals; D001672:Biocompatible Materials / Q000009:adverse effects / Q000138:chemical synthesis; D002130:Calcium Phosphates / Q000009:adverse effects* / Q000737:chemistry; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D022781:Hepatocytes / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D008422:Materials Testing; D008657:Metabolic Clearance Rate / Q000502:physiology; D053769:Nanocapsules / Q000009:adverse effects* / Q000737:chemistry*; D054118:Nanospheres / Q000009:adverse effects* / Q000737:chemistry; D010316:Particle Size; D010718:Phosphatidylserines / Q000009:adverse effects / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry / Q000633:toxicity; D011817:Rabbits; D015996:Survival Rate; D014018:Tissue Distribution; D015027:Zebrafish / Q000502:physiology*
D001672:Biocompatible Materials; D002130:Calcium Phosphates; D053769:Nanocapsules; D010718:Phosphatidylserines; D011092:Polyethylene Glycols; C020243:calcium phosphate
Biocompatible; biodistribution; cytotoxicity; drug carrier; nanospheres