Carriage of antibiotic-resistant Haemophilus influenzae strains in children undergoing adenotonsillectomy.
Haemophilus influenzae is one of the major pathogenic bacteria in upper respiratory tract of children. In this study, the presence of various H. influenzae genotypes were followed-up for at least 13 weeks, starting from one week before surgery. Forty-one children with chronic adenoid hypertrophy were prospectively enrolled to the study. The consecutive swabs of adenoid and tonsils, two before adenotonsillectomy and two after the surgery together with homogenates of adenotonsillar tissues and lysates of the CD14(+) cells fraction were acquired from 34 children undergoing adenotonsillectomy. Up to ten isolates from each patient at each collection period were genotyped using a PFGE method and their capsular type and antibiotic susceptibility was determined. Of the 1001 isolates examined, we identified 325 isolates grouped into 16 persistent genotypes, which colonized throats for more than seven weeks and were not eliminated by the surgery. The other 506 isolates grouped into 48 transient genotypes that had been eliminated by the surgery. The resistance to ampicillin were found in 23.8% of the transient strains, and 4.7% of the newly acquired strains following the surgical intervention. In contrast, none of the persistent strains were resistant to ampicillin; however, these strains showed apparently higher level of resistance to co-trimoxazole when compared to transient strains. The transient and persistent strains did not significantly differ in bacterial viability in the biofilms formed in vitro. Some of the strains were identified in two or three different patients and were considered as the strains circulating in the region between 2010 and 2012.
Department of Microbiology, Military Institute of Hygiene and Epidemiology, Kozielska 4, 01-163 Warsaw, Poland. Electronic address: e.trafny@wihe.waw.pl.
Elżbieta A
EA
Trafny
Department of Otolaryngology, Children's Hospital, Niekłańska 4/24, 03-924 Warsaw, Poland.
Olga
O
Olszewska-Sosińska
Department of Microbiology, Military Institute of Hygiene and Epidemiology, Kozielska 4, 01-163 Warsaw, Poland.
Małgorzata
M
Antos-Bielska
Department of Microbiology, Military Institute of Hygiene and Epidemiology, Kozielska 4, 01-163 Warsaw, Poland.
Krystyna
K
Kozłowska
Department of Microbiology, Military Institute of Hygiene and Epidemiology, Kozielska 4, 01-163 Warsaw, Poland.
Małgorzata
M
Stępińska
Department of Laboratory Diagnostics, Children's Hospital, Niekłańska 4/24, 03-924 Warsaw, Poland.
Magdalena
M
Lau-Dworak
Department of Otolaryngology, Children's Hospital, Niekłańska 4/24, 03-924 Warsaw, Poland.
Beata
B
Zielnik-Jurkiewicz
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002353:Carrier State / Q000453:epidemiology* / Q000382:microbiology; D002648:Child; D002675:Child, Preschool; D024881:Drug Resistance, Bacterial; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D005838:Genotype; D006192:Haemophilus Infections / Q000453:epidemiology* / Q000382:microbiology; D006193:Haemophilus influenzae / Q000145:classification / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008137:Longitudinal Studies; D008297:Male; D058889:Molecular Typing; D010614:Pharynx / Q000382:microbiology*; D011446:Prospective Studies; D014068:Tonsillectomy
D000900:Anti-Bacterial Agents
Antibiotic susceptibility; Chronic adenoiditis/tonsillitis; Hypertrophy of adenoid/tonsils; Persistent genotypes