Evaluation of antioxidant and antimicrobial activities and phenolic profile for Hyssopus officinalis, Ocimum basilicum and Teucrium chamaedrys.
This study was designed to examine the in vitro antioxidant and antimicrobial activities and to characterize the polyphenolic composition of the ethanolic extracts of Hyssopus officinalis, Ocimum basilicum and Teucrium chamaedrys. Qualitative and quantitative analysis of the major phenolic compounds were conducted using high-performance liquid chromatography coupled to mass spectrometry (HPLC-MS). The total polyphenols, caffeic acid derivatives and flavonoids content was spectrophotometrically determined. The phenolic profile showed the presence of phenolic acid derivatives (caftaric, gentisic, caffeic, p-coumaric, chlorogenic and ferulic acids), flavonoid glycosides (rutin, isoquercitrin and quercitrin) and free flavonoid aglycons (luteolin, quercetin), in different concentrations. DPPH radical scavenging assay, Trolox equivalent antioxidant capacity (TEAC) method, hemoglobin ascorbate peroxidase activity inhibition (HAPX) assay, and electron paramagnetic resonance (EPR) radicals detection were employed, revealing several aspects of the antioxidant activities of these species. The antimicrobial tests were performed using the disk diffusion assay. These extracts contained a large amount of the polyphenolic compounds (77.72, 175.57, and 243.65 mg/g, respectively), and they showed a good antioxidant activity, as witnessed by a number of methods. T. chamaedrys had a high antimicrobial activity. Besides their antioxidant activity, the antimicrobial effect of these extracts confirms the biological activities of these herbal medicinal products.
Department of Pharmaceutical Technology and Biopharmaceutics, Iuliu Hatieganu University of Medicine and Pharmacy, 12 I. Creanga Street, Cluj-Napoca 400010, Romania. laurian.vlase@umfcluj.ro.
Laurian
L
Vlase
Department of Pharmacognosy, Iuliu Hatieganu University of Medicine and Pharmacy, 12 I. Creanga Street, Cluj-Napoca 400010, Romania. dbenedec@umfcluj.ro.
Daniela
D
Benedec
Department of Pharmacognosy, Iuliu Hatieganu University of Medicine and Pharmacy, 12 I. Creanga Street, Cluj-Napoca 400010, Romania. dhanganu@umfcluj.ro.
Daniela
D
Hanganu
Department of Physics, Babes-Bolyai University, 1 M. Kogalniceanu Street, Cluj-Napoca 400084, Romania. grigore.damian@phys.ubbcluj.ro.
Grigore
G
Damian
Department of Physics, Babes-Bolyai University, 1 M. Kogalniceanu Street, Cluj-Napoca 400084, Romania. bapo25@yahoo.it.
Ioan
I
Csillag
Department of Physiopathology, University of Agricultural Sciences and Veterinary Medicine, 3-5 Mănăştur Street, Cluj-Napoca 400372, Romania. bogdan.sevastre@usamvcluj.ro.
Bogdan
B
Sevastre
Department of Chemistry and Chemical Engineering Babes-Bolyai University, 11 A. Janos Street, Cluj-Napoca 400028, Romania. augustinmot@chem.ubbcluj.ro.
Augustin C
AC
Mot
Department of Chemistry and Chemical Engineering Babes-Bolyai University, 11 A. Janos Street, Cluj-Napoca 400028, Romania.
Radu
R
Silaghi-Dumitrescu
Family Medicine, Department M3 Clinical Sciences Internal Medicine, University of Medicine and Pharmacy, 38 G. Marinescu Street, Târgu Mures 540139, Romania. ioan.tilea@umftgm.ro.
Ioan
I
Tilea
D000890:Anti-Infective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects*; D019686:Lamiaceae / Q000737:chemistry; D018649:Ocimum basilicum / Q000737:chemistry; D010636:Phenols / Q000302:isolation & purification* / Q000494:pharmacology; D010936:Plant Extracts; D031367:Teucrium / Q000737:chemistry
D000890:Anti-Infective Agents; D000975:Antioxidants; D010636:Phenols; D010936:Plant Extracts