Alternative variants of human HYDIN are novel cancer-associated antigens recognized by adaptive immunity.
A mutation in the hydin gene has been recently described as one possible mechanism leading to lethal congenital hydrocephalus in mice, and a similar defect is proposed to be involved in an autosomal recessive form of hydrocephalus in human. Here, we report for the first time on the cancer association and immunogenicity of two HYDIN variants in humans. One is a previously described sequence derived from the chromosome 1 gene copy, that is, KIAA1864. The second is encoded by a novel alternative transcript originating from the chromosome 16, which we identified by immunoscreening of a testis-derived cDNA expression library with sera of patients with colorectal cancer, and called MO-TES391. Both variants are targeted by immunoglobulin G antibodies in a significant subset of cancer patients but only rarely in healthy donors. Moreover, we identify HLA-A*0201-restricted sequences derived from MO-TES391 and KIAA1864, which are specifically recognized by human cytotoxic CD8(+) T cells. Taken together, these results suggest frequent and coordinated adaptive immune responses against HYDIN variants in patients with cancer and propose HYDIN as a novel cancer-associated antigen.
Authors' Affiliations: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.
Karoline
K
Laske
Yuriy V
YV
Shebzukhov
Ludger
L
Grosse-Hovest
Dmitry V
DV
Kuprash
Svetlana V
SV
Khlgatian
Ekaterina P
EP
Koroleva
Alexey Y
AY
Sazykin
Dmitry N
DN
Penkov
Pavel V
PV
Belousov
Stefan
S
Stevanovic
Verona
V
Vass
Steffen
S
Walter
David
D
Eisel
Barbara D
BD
Schmid-Horch
Sergei A
SA
Nedospasov
Hans-Georg
HG
Rammensee
Cécile
C
Gouttefangeas
D056704:Adaptive Immunity / Q000235:genetics*; D017398:Alternative Splicing; D000818:Animals; D000951:Antigens, Neoplasm / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002878:Chromosomes, Human, Pair 1; D002885:Chromosomes, Human, Pair 16; D015789:HLA-A2 Antigen / Q000378:metabolism; D006801:Humans; D051379:Mice; D008840:Microfilament Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D009154:Mutation; D009369:Neoplasms / Q000276:immunology; D012333:RNA, Messenger / Q000276:immunology / Q000378:metabolism
D000951:Antigens, Neoplasm; C435939:HLA-A*02:01 antigen; D015789:HLA-A2 Antigen; C000599596:HYDIN protein, human; D008840:Microfilament Proteins; D012333:RNA, Messenger