Production of bone marrow, liver, thymus (BLT) humanized mice on the C57BL/6 Rag2(-/-)γc(-/-)CD47(-/-) background.
C57BL/6 Rag2(-/-)γc(-/-)CD47(-/-) triple-knockout mice engrafted with fetal human bone marrow, liver, thymus (TKO-BLT) not only develop high levels of multi-lineage hematopoiesis but also organized lymphoid tissues including mesenteric lymph nodes, splenic follicles and gut-associated lymphoid tissues of human origin. A unique advantage of these mice is that they sustain human cell and tissue engraftment long-term without the development of graft versus host disease. Thus they can be used for long-term studies not previously feasible with other models. The production of TKO-BLT mice to obtain healthy mice with high level reconstitution of human cells and tissues requires specialized methods that are presented in detail.
Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, NIAID, NIH, Hamilton, MT 59840, USA.
Kerry J
KJ
Lavender
Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, NIAID, NIH, Hamilton, MT 59840, USA.
Ronald J
RJ
Messer
Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, NIAID, NIH, Hamilton, MT 59840, USA.
Brent
B
Race
Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, NIAID, NIH, Hamilton, MT 59840, USA. Electronic address: khasenkrug@nih.gov.
Kim J
KJ
Hasenkrug
D000818:Animals; D001853:Bone Marrow / Q000276:immunology*; D004268:DNA-Binding Proteins / Q000235:genetics; D055786:Gene Knockout Techniques / Q000379:methods; D006086:Graft vs Host Disease / Q000276:immunology; D006801:Humans; D053631:Interleukin Receptor Common gamma Subunit / Q000235:genetics; D008099:Liver / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009687:Nuclear Proteins / Q000235:genetics; D013950:Thymus Gland / Q000276:immunology*; D013997:Time Factors; D014183:Transplantation, Heterologous
D004268:DNA-Binding Proteins; D053631:Interleukin Receptor Common gamma Subunit; D009687:Nuclear Proteins; C499311:RAG2 protein, human
BLT humanized mice; C57BL/6 Rag2(−/−)γ(c)(−/−)CD47(−/−); Hematopoiesis