Improving drug accumulation and photothermal efficacy in tumor depending on size of ICG loaded lipid-polymer nanoparticles.
A key challenge to strengthen anti-tumor efficacy is to improve drug accumulation in tumors through size control. To explore the biodistribution and tumor accumulation of nanoparticles, we developed indocyanine green (ICG) loaded poly (lactic-co-glycolic acid) (PLGA) -lecithin-polyethylene glycol (PEG) core-shell nanoparticles (INPs) with 39 nm, 68 nm and 116 nm via single-step nanoprecipitation. These INPs exhibited good monodispersity, excellent fluorescence and size stability, and enhanced temperature response after laser irradiation. Through cell uptake and photothermal efficiency in vitro, we demonstrated that 39 nm INPs were more easily be absorbed by pancreatic carcinoma tumor cells (BxPC-3) and showed better photothermal damage than that of 68 nm and 116 nm size of INPs. Simultaneously, the fluorescence of INPs offered a real-time imaging monitor for subcellular locating and in vivo metabolic distribution. Near-infrared imaging in vivo and photothermal therapy illustrated that 68 nm INPs showed the strongest efficiency to suppress tumor growth due to abundant accumulation in BxPC-3 xenograft tumor model. The findings revealed that a nontoxic, size-dependent, theranostic INPs model was built for in vivo cancer imaging and photothermal therapy without adverse effect.
Guangdong Key Laboratory of Nanomedicine & Shenzhen Key Laboratory of Cancer Nanotechnology, Institute of Biomedicine and Biotechnology, Chinese Academy of Sciences, Shenzhen 518055, PR China.
Pengfei
P
Zhao
Guangdong Key Laboratory of Nanomedicine & Shenzhen Key Laboratory of Cancer Nanotechnology, Institute of Biomedicine and Biotechnology, Chinese Academy of Sciences, Shenzhen 518055, PR China; Department of Chemistry, Guangdong Medical College, Dongguan 523808, PR China.
Mingbin
M
Zheng
Guangdong Key Laboratory of Nanomedicine & Shenzhen Key Laboratory of Cancer Nanotechnology, Institute of Biomedicine and Biotechnology, Chinese Academy of Sciences, Shenzhen 518055, PR China.
Caixia
C
Yue
Guangdong Key Laboratory of Nanomedicine & Shenzhen Key Laboratory of Cancer Nanotechnology, Institute of Biomedicine and Biotechnology, Chinese Academy of Sciences, Shenzhen 518055, PR China.
Zhenyu
Z
Luo
Guangdong Key Laboratory of Nanomedicine & Shenzhen Key Laboratory of Cancer Nanotechnology, Institute of Biomedicine and Biotechnology, Chinese Academy of Sciences, Shenzhen 518055, PR China.
Ping
P
Gong
Guangdong Key Laboratory of Nanomedicine & Shenzhen Key Laboratory of Cancer Nanotechnology, Institute of Biomedicine and Biotechnology, Chinese Academy of Sciences, Shenzhen 518055, PR China.
Guanhui
G
Gao
Guangdong Key Laboratory of Nanomedicine & Shenzhen Key Laboratory of Cancer Nanotechnology, Institute of Biomedicine and Biotechnology, Chinese Academy of Sciences, Shenzhen 518055, PR China.
Zonghai
Z
Sheng
Guangdong Key Laboratory of Nanomedicine & Shenzhen Key Laboratory of Cancer Nanotechnology, Institute of Biomedicine and Biotechnology, Chinese Academy of Sciences, Shenzhen 518055, PR China.
Cuifang
C
Zheng
Guangdong Key Laboratory of Nanomedicine & Shenzhen Key Laboratory of Cancer Nanotechnology, Institute of Biomedicine and Biotechnology, Chinese Academy of Sciences, Shenzhen 518055, PR China. Electronic address: lt.cai@siat.ac.cn.
Lintao
L
Cai
D000818:Animals; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D004058:Diffusion; D005260:Female; D007208:Indocyanine Green / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics*; D008055:Lipids / Q000737:chemistry*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D053769:Nanocapsules / Q000008:administration & dosage* / Q000737:chemistry* / Q000648:ultrastructure; D010190:Pancreatic Neoplasms / Q000473:pathology / Q000628:therapy*; D010316:Particle Size; D017319:Photosensitizing Agents / Q000008:administration & dosage / Q000493:pharmacokinetics; D010789:Phototherapy / Q000379:methods*; D011108:Polymers / Q000737:chemistry; D016896:Treatment Outcome
D008055:Lipids; D053769:Nanocapsules; D017319:Photosensitizing Agents; D011108:Polymers; D007208:Indocyanine Green
Biodistribution; Endocytosis; Indocyanine green; Lipid-polymer nanoparticle; Photothermal therapy