Proteomic study of differential protein expression in mouse lung tissues after aerosolized ricin poisoning.
Ricin is one of the most poisonous natural toxins from plants and is classified as a Class B biological threat pathogen by the Centers for Disease Control and Prevention (CDC) of U.S.A. Ricin exposure can occur through oral or aerosol routes. Ricin poisoning has a rapid onset and a short incubation period. There is no effective treatment for ricin poisoning. In this study, an aerosolized ricin-exposed mouse model was developed and the pathology was investigated. The protein expression profile in the ricin-poisoned mouse lung tissue was analyzed using proteomic techniques to determine the proteins that were closely related to the toxicity of ricin. 2D gel electrophoresis, mass spectrometry and subsequent biological functional analysis revealed that six proteins including Apoa1 apolipoprotein, Ywhaz 14-3-3 protein, Prdx6 Uncharacterized Protein, Selenium-binding protein 1, HMGB1, and DPYL-2, were highly related to ricin poisoning.
Institute of Military Veterinary, Academy of Military Medical Sciences, 666 West Liuying Road, Changchun 130122, Jilin, China. guozd@foxmail.com.
Zhendong
Z
Guo
Clinical Department of General Hospital of Beijing Military Region, Beijing 101149, China. hc1345@126.com.
Chao
C
Han
Medical Information Center of Chinese PLA General Hospital, Beijing 100853, China. spna7@126.com.
Jiajun
J
Du
Institute of Military Veterinary, Academy of Military Medical Sciences, 666 West Liuying Road, Changchun 130122, Jilin, China. zhaosy@foxmail.com.
Siyan
S
Zhao
Institute of Military Veterinary, Academy of Military Medical Sciences, 666 West Liuying Road, Changchun 130122, Jilin, China. fyyinging@163.com.
Yingying
Y
Fu
Institute of Military Veterinary, Academy of Military Medical Sciences, 666 West Liuying Road, Changchun 130122, Jilin, China. zhengguanyu1@163.com.
Guanyu
G
Zheng
Institute of Military Veterinary, Academy of Military Medical Sciences, 666 West Liuying Road, Changchun 130122, Jilin, China. ycsun_73@126.com.
Yucheng
Y
Sun
Institute of Military Veterinary, Academy of Military Medical Sciences, 666 West Liuying Road, Changchun 130122, Jilin, China. yizhang818@163.com.
Yi
Y
Zhang
Institute of Military Veterinary, Academy of Military Medical Sciences, 666 West Liuying Road, Changchun 130122, Jilin, China. wsliu_1101@126.com.
Wensen
W
Liu
Institute of Military Veterinary, Academy of Military Medical Sciences, 666 West Liuying Road, Changchun 130122, Jilin, China. jywan_1973@163.com.
Jiayu
J
Wan
Institute of Military Veterinary, Academy of Military Medical Sciences, 666 West Liuying Road, Changchun 130122, Jilin, China. qianj1970@126.com.
Jun
J
Qian
Institute of Military Veterinary, Academy of Military Medical Sciences, 666 West Liuying Road, Changchun 130122, Jilin, China. liulinna7@126.com.
Linna
L
Liu
D000336:Aerosols / Q000506:poisoning; D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D008168:Lung / Q000187:drug effects / Q000473:pathology*; D055370:Lung Injury / Q000139:chemically induced* / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011506:Proteins / Q000032:analysis*; D040901:Proteomics; D012276:Ricin / Q000506:poisoning*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000336:Aerosols; D011506:Proteins; D012276:Ricin