Alterations in cholesterol and ganglioside GM1 content of lipid rafts in platelets from patients with Alzheimer disease.
The aim of this study was to investigate the changes in the protein, cholesterol, and ganglioside GM1 content of lipid rafts in platelets from patients with Alzheimer disease (AD), and identify potential blood biomarkers of the disease. A total of 31 Chinese patients with AD and 31 aged-matched control subjects were selected. Lipid rafts were isolated from platelets using Optiprep gradient centrifugation. The protein content of lipid rafts was evaluated using Micro BCA assay, the cholesterol content using molecular probes, ganglioside GM1 content using colorimetry and dot-blotting analysis. The results showed that the cholesterol and ganglioside GM1 content of lipid rafts from platelets was significantly higher in patients with AD than aged-matched control subjects, whereas the protein content of lipid rafts did not show any differences between the 2 groups. These results indicate that the increases in the cholesterol and ganglioside GM1 content of lipid rafts from the platelets of patients with AD might serve as a biochemical adjunct to the clinical diagnosis of AD.
*Department of Neurology, the First Affiliated Hospital of China Medical University †Department of Neurology, Shenyang Fifth People Hospital ‡Department of Developmental Cell Biology, Key Laboratory of Cell Biology, Ministry of Public Health, Key Laboratory of Medical Cell Biology, Ministry of Education, China Medical University, Shenyang, 110001 China.
Li
L
Liu
Ke
K
Zhang
Liang
L
Tan
Yu-Hua
YH
Chen
Yun-Peng
YP
Cao
D000368:Aged; D000544:Alzheimer Disease / Q000097:blood* / Q000175:diagnosis* / Q000523:psychology; D044466:Asian People / Q000523:psychology; D015415:Biomarkers / Q000097:blood; D002784:Cholesterol / Q000097:blood*; D005260:Female; D005677:G(M1) Ganglioside / Q000097:blood*; D006801:Humans; D008297:Male; D021962:Membrane Microdomains / Q000378:metabolism*; D008875:Middle Aged
D015415:Biomarkers; D005677:G(M1) Ganglioside; D002784:Cholesterol