Evaluation on genotoxicity and teratogenicity of aqueous extract from Cyclocarya paliurus leaves.
Tremendous attentions have been attracted to the foods labeled with natural, green, organic, and nuisanceless conception of healthy diet. Therefore, it is of great significance to establish relative defining guidance for safe assessment of botanicals. Cyclocarya paliurus (Batal.) Iljinsk (family Cyclocaryaceae), called sweet tea tree, is a well-known edible and medicinal plant, which has been widely used in China as drug formulation for the treatment of hypertension and diabetes. Despite its benefits, no reports have been described on the safe assessment of C. paliurus leaves aqueous extract. In this study, we have conducted the genotoxicity assay (including Ames test, bone marrow polychromatic erythrocyte micronucleus test, and sperm abnormality test in mice) and traditional teratogenicity assay in rats (maternal toxicity, embryo toxicity, and teratogenicity test) to assess the genetic and teratogenic safety of aqueous extracts from C. paliurus leaves. Results of each assay show that the highest dose of C. paliurus leaves aqueous extract is considered relatively nonmutagenic and nonteratogenic, revealing that C. paliurus leaves possess safety and quality as a functional additional ingredient in food.
Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forest University, Beijing 100083, China.
Lihong
L
Deng
Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forest University, Beijing 100083, China.
Jiandu
J
Lei
Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forest University, Beijing 100083, China.
Jing
J
He
Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forest University, Beijing 100083, China.
Jing
J
Liu
Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forest University, Beijing 100083, China.
Luying
L
Wang
Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
Rui
0000-0002-7488-4473
R
Zhang
Hunan Cyclocarya paliurus Technology Development Co. Ltd, Shuiling, Hunan 411100, China.
Xinhua
X
Liu
Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
Yun
Y
Liu
D000818:Animals; D029525:Juglandaceae / Q000737:chemistry*; D008297:Male; D051379:Mice; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D010936:Plant Extracts / Q000633:toxicity*; D018515:Plant Leaves / Q000737:chemistry*; D013723:Teratogens / Q000633:toxicity*
D009153:Mutagens; D010936:Plant Extracts; D013723:Teratogens