Trenbolone acetate metabolites promote ovarian growth and development in adult Japanese medaka (Oryzias latipes).
Trenbolone acetate, a synthetic androgen, has been used as a growth promoter in beef cattle in the US since 1987. While several teleost studies have investigated the masculinization effects of the metabolite 17β-trenbolone, few have focused on the reproductive impacts of all three trenbolone acetate (TBA) metabolites including trendione. Adult female medaka (Oryzias latipes) were exposed to TBA metabolites (10, 100, and 1000ng/L) for 14days (n=3). Histological examination revealed that TBA metabolites (1000ng/L) significantly reduced the percentage of primary ovarian follicles and increased the percentage of vitellogenic follicles compared to control fish. 17α-Trenbolone significantly increased whereas trendione reduced whole body levels of estradiol-17β. Testosterone was significantly reduced by trendione treatment and only the highest dose of 17β-trenbolone and lowest dose of trendione altered 11-ketotestosterone. Additionally, TBA metabolites may be further broken down and/or metabolized or converted by the animal influencing both sex steroid levels and ovarian development.
Department of Environmental Sciences, University of California, Riverside, CA 92521, United States. Electronic address: kforsgren@fullerton.edu.
Kristy L
KL
Forsgren
Department of Civil and Environmental Engineering, University of Iowa, Iowa City, IA 52242, United States.
Shen
S
Qu
Department of Environmental Sciences, University of California, Riverside, CA 92521, United States.
Ramon
R
Lavado
Department of Civil and Environmental Engineering, University of Iowa, Iowa City, IA 52242, United States.
David
D
Cwiertny
Department of Environmental Sciences, University of California, Riverside, CA 92521, United States.
Daniel
D
Schlenk
D000818:Animals; D001681:Biological Assay; D001711:Biotransformation / Q000187:drug effects; D002417:Cattle; D004963:Estrenes / Q000494:pharmacology; D005260:Female; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism; D017686:Oncorhynchus mykiss; D009990:Oryzias / Q000254:growth & development / Q000378:metabolism; D006080:Ovarian Follicle / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D010053:Ovary / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013256:Steroids / Q000378:metabolism; D014204:Trenbolone Acetate / Q000378:metabolism* / Q000494:pharmacology*; D014819:Vitellogenins / Q000235:genetics / Q000378:metabolism
D004963:Estrenes; D012333:RNA, Messenger; D013256:Steroids; D014819:Vitellogenins; C070427:trendione; D014204:Trenbolone Acetate
Aquatic toxicology; Reproduction; Sex steroids; Trenbolone acetate metabolites