CTX-M-type extended-spectrum β-lactamase-producing Klebsiella pneumoniae isolated from cases of bovine mastitis in Japan.
Three Klebsiella pneumoniae isolates producing extended-spectrum beta-lactamase (ESBL) were obtained from three dairy cows with clinical mastitis in two farms in western Japan. Two of the 3 isolates from cows in different farms were able to transfer plasmids carrying the blaCTX-M-2 gene to Escherichia coli recipient. Pulsed-field gel electrophoresis (PFGE) patterns of the 2 isolates were different from each other, although restricted-fragment patterns of the two conjugative plasmids were similar to each other. Additionally, PCR-based replicon typing revealed that both the plasmids belonged to type Inc.T. These results suggest that ESBL-encoding genes can be distributed in bacteria on dairy farms through the plasmids.
Tottori Prefectural Kurayoshi Livestock Hygiene Service Center, 2-132 Seidani-cho, Kurayoshi, Tottori 682-0017, Japan.
Nobukazu
N
Saishu
Hiroichi
H
Ozaki
Toshiyuki
T
Murase
D000818:Animals; D002417:Cattle; D017931:DNA Primers / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D004926:Escherichia coli; D005260:Female; D007564:Japan; D007711:Klebsiella pneumoniae / Q000201:enzymology*; D008414:Mastitis, Bovine / Q000382:microbiology*; D010957:Plasmids / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
D017931:DNA Primers; C515384:beta-lactamase CTX-M-2, Klebsiella pneumoniae; D001618:beta-Lactamases