A label-free fluorescence detection strategy for lysozyme assay using CuInS₂ quantum dots.
We have developed a simple and efficient fluorescence detection system for lysozyme utilizing I-III-VI type Cu-In-S ternary quantum dots (CuInS2 QDs) as the probe. Water-soluble near-infrared CuInS2 QDs capped with 3-mercaptopropionic acid were directly synthesized by using a hydrothermal method. Poly(dimethyl diallyl ammonium chloride), as a cationic polyelectrolyte, could bind to 3-mercaptopropionic acid-capped CuInS2 QDs via electrostatic interactions that would lead to fluorescence quenching of CuInS2 QDs. And the addition of a lysozyme aptamer could effectively turn on the quenched fluorescence due to the stronger interaction between the cationic polyelectrolyte and negatively charged aptamer. The addition of lysozyme would result in fluorescence quenching of CuInS2 QDs again due to the specific binding between lysozyme and the lysozyme aptamer. Therefore, this label-free fluorescence system could be applied to selectively detect lysozyme.
Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, China. suxg@jlu.edu.cn.
Siyu
S
Liu
Weidan
W
Na
Shu
S
Pang
Fanping
F
Shi
Xingguang
X
Su
D003300:Copper / Q000737:chemistry*; D007204:Indium / Q000737:chemistry*; D009113:Muramidase / Q000032:analysis*; D045663:Quantum Dots; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013440:Sulfides / Q000737:chemistry*
D013440:Sulfides; D007204:Indium; D003300:Copper; D009113:Muramidase