Antioxidant activity and delayed aging effects of hot water extract from Chamaecyparis obtusa var. formosana leaves.
The antioxidant activity and delayed aging effects of hot water extracts from leaves of Chamaecyparis obtusa var. formosana were investigated. Free radical, superoxide radical scavenging, and total phenolic content assays were employed to evaluate the in vitro activities of the extracts. In addition, in vivo assays using the nematode Caenorhabditis elegans were also performed in this study. The results showed that among all soluble fractions obtained from the extracts, the ethyl acetate-soluble fraction has the best in vitro and in vivo antioxidant activities. Moreover, it decreased significantly the deposition of lipofuscin (aging pigment) and extended the lifespan of C. elegans. Bioactivity-guided fractionation yielded six potent antioxidant constituents from the ethyl acetate-soluble fraction, namely, catechin, quercetin, quercetin-3-O-α-rhamnoyranoside, myricetin-3-O-α-rhamnoyranoside, vanillic acid, and 4-hydroxybenzoic acid. Quercetin-3-O-α-rhamnoyranoside pretreatment showed the highest survival of C. elegans upon juglone exposure. Taken together, the results revealed that hot water extracts from C. obtusa var. formosana leaves have the potential to be used as a source for antioxidant or delayed aging health food.
School of Forest and Resource Conservation, National Taiwan University , Taipei 10617, Taiwan.
Szu-Chin
SC
Cheng
Wen-Hsuan
WH
Li
Yeu-Ching
YC
Shi
Pei-Ling
PL
Yen
Huan-You
HY
Lin
Vivian Hsiu-Chuan
VH
Liao
Shang-Tzen
ST
Chang
D000375:Aging / Q000187:drug effects*; D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D017173:Caenorhabditis elegans / Q000187:drug effects / Q000254:growth & development*; D029780:Chamaecyparis / Q000737:chemistry*; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry
D000975:Antioxidants; D010936:Plant Extracts
Caenorhabditis elegans; Chamaecyparis obtusa var. formosana; antioxidant activity; delayed aging effect