Skeletal muscle plasticity induced by seasonal acclimatization in carp involves differential expression of rRNA and molecules that epigenetically regulate its synthesis.
Ribosomal biogenesis controls cellular growth in living organisms, with the rate-limiting step of this process being the transcription of ribosomal DNA (rDNA). Considering that epigenetic mechanisms allow an organism to respond to environmental changes, the expression in muscle of several molecules that regulate epigenetic rRNA synthesis, as well as rDNA transcription, were evaluated during the seasonal acclimatization of the carp. First, the nucleotide sequences encoding the components forming the NoRC (ttf-I, tip5) and eNoSC (sirt1, nml, suv39h1), two chromatin remodeling complexes that silence rRNA synthesis, as well as the sequence of ubf1, a key regulator of rDNA transcription, were obtained. Subsequently the transcriptional regulation of the aforementioned molecules, and other key molecules involved in rRNA synthesis (mh2a1, mh2a2, h2a.z, h2a.z.7, nuc, p80), was assessed. The carp sequences for TTF-I, TIP5, SIRT1, NML, SUV39H1, and UBF1 showed a high conservation of domains and key amino acids in comparison with other fish and higher vertebrates. The mRNA contents in muscle for ttf-I, tip5, sirt1, nml, suv39h1, mh2a1, mh2a.z, and nuc were up-regulated during winter in comparison with summer, whereas the mRNA levels of mh2a2, ubf1, and p80 were down-regulated. Also, the contents of molecules involved in processing the rRNA (snoRNAs) and pRNA, a stabilizer of NoRC complex, were analyzed, finding that these non-coding RNAs were not affected by seasonal acclimatization. These results suggest that variations in the expression of rRNA and the molecules that epigenetically regulate its synthesis are contributing to the muscle plasticity induced by seasonal acclimatization in carp.
Laboratorio de Biotecnologia Molecular, Departamento de Ciencias Biologicas, Facultad de Ciencias Biologicas, Universidad Andres Bello, Av.Republica 217, Santiago, Chile; Interdisciplinary Center for Aquaculture Research (INCAR), Lamas 1290 Victor, PO Box 160-C, Concepción, Chile. Electronic address: edua.fuentes@uandresbello.edu.
Eduardo N
EN
Fuentes
Laboratorio de Biotecnologia Molecular, Departamento de Ciencias Biologicas, Facultad de Ciencias Biologicas, Universidad Andres Bello, Av.Republica 217, Santiago, Chile.
Rodrigo
R
Zuloaga
Laboratorio de Biología Celular y Molecular, Departamento de Ciencias Biologicas, Facultad de Ciencias Biologicas Universidad Andres Bello, Quillota 980, Viña del Mar, Chile.
Gino
G
Nardocci
Laboratorio de Biología Celular y Molecular, Departamento de Ciencias Biologicas, Facultad de Ciencias Biologicas Universidad Andres Bello, Quillota 980, Viña del Mar, Chile.
Catalina
C
Fernandez de la Reguera
Laboratorio de Biología Celular y Molecular, Departamento de Ciencias Biologicas, Facultad de Ciencias Biologicas Universidad Andres Bello, Quillota 980, Viña del Mar, Chile.
Nicolas
N
Simonet
Laboratorio de Biología Celular y Molecular, Departamento de Ciencias Biologicas, Facultad de Ciencias Biologicas Universidad Andres Bello, Quillota 980, Viña del Mar, Chile.
Robinson
R
Fumeron
Laboratorio de Biotecnologia Molecular, Departamento de Ciencias Biologicas, Facultad de Ciencias Biologicas, Universidad Andres Bello, Av.Republica 217, Santiago, Chile; Interdisciplinary Center for Aquaculture Research (INCAR), Lamas 1290 Victor, PO Box 160-C, Concepción, Chile.
Juan Antonio
JA
Valdes
Laboratorio de Biotecnologia Molecular, Departamento de Ciencias Biologicas, Facultad de Ciencias Biologicas, Universidad Andres Bello, Av.Republica 217, Santiago, Chile; Interdisciplinary Center for Aquaculture Research (INCAR), Lamas 1290 Victor, PO Box 160-C, Concepción, Chile.
Alfredo
A
Molina
Interdisciplinary Center for Aquaculture Research (INCAR), Lamas 1290 Victor, PO Box 160-C, Concepción, Chile; Laboratorio de Biología Celular y Molecular, Departamento de Ciencias Biologicas, Facultad de Ciencias Biologicas Universidad Andres Bello, Quillota 980, Viña del Mar, Chile. Electronic address: marcoalvarez@unab.cl.
Marco
M
Alvarez
D000064:Acclimatization / Q000502:physiology; D000818:Animals; D002347:Carps / Q000502:physiology*; D042002:Chromatin Assembly and Disassembly; D002868:Chromosomal Proteins, Non-Histone; D003001:Cloning, Molecular; D044127:Epigenesis, Genetic; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D006657:Histones / Q000235:genetics / Q000378:metabolism; D018482:Muscle, Skeletal / Q000502:physiology*; D035463:Pol1 Transcription Initiation Complex Proteins / Q000235:genetics; D012335:RNA, Ribosomal / Q000378:metabolism*; D012621:Seasons; D056564:Sirtuin 1 / Q000235:genetics
D002868:Chromosomal Proteins, Non-Histone; D029941:Fish Proteins; D006657:Histones; D035463:Pol1 Transcription Initiation Complex Proteins; D012335:RNA, Ribosomal; D056564:Sirtuin 1
Epigenetics; Fish; Muscle; Seasonal acclimatization; rDNA transcription