Studies on production and biological potential of prodigiosin by Serratia marcescens.
Efficacy of Serratia marcescens for pigment production and biological activity was investigated. Natural substrates like sweet potato, mahua flower extract (Madhuca latifolia L.), and sesam at different concentrations were taken. As a carbon source microorganism favored potato powder was followed by sesam and mannitol, and as nitrogen source casein hydrolysate was followed by yeast and malt extract. The effect of inorganic salts on pigment production was also studied. At final optimized composition of suitable carbon, nitrogen source, and trace materials and at suitable physiological conditions, prodigiosin production was 4.8 g L(-1). The isolated pigment showed antimicrobial activity against different pathogenic bacteria and fungi. Extracted pigment was characterized by spectroscopy, Fourier transform infrared (FTIR), and thin layer chromatography (TLC) which confirm production of biological compound prodigiosin. This study suggests that use of sweet potato powder and casein can be a potential alternative bioresource for commercial production of pigment prodigiosin.
School of Life Sciences, North Maharashtra University, Jalgaon, 425001, Maharashtra, India.
Rahul K
RK
Suryawanshi
Chandrashekhar D
CD
Patil
Hemant P
HP
Borase
Bipinchandra K
BK
Salunke
Satish V
SV
Patil
D000388:Air; D000890:Anti-Infective Agents / Q000032:analysis / Q000378:metabolism* / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D001709:Biotechnology / Q000379:methods*; D002119:Calcium Carbonate / Q000494:pharmacology; D002244:Carbon / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005658:Fungi / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D009584:Nitrogen / Q000494:pharmacology; D011353:Prodigiosin / Q000032:analysis / Q000096:biosynthesis* / Q000494:pharmacology; D012706:Serratia marcescens / Q000187:drug effects / Q000378:metabolism*; D013696:Temperature
D000890:Anti-Infective Agents; D002244:Carbon; D002119:Calcium Carbonate; D009584:Nitrogen; D011353:Prodigiosin