Optical coherence tomography can assess skeletal muscle tissue from mouse models of muscular dystrophy by parametric imaging of the attenuation coefficient.
We present the assessment of ex vivo mouse muscle tissue by quantitative parametric imaging of the near-infrared attenuation coefficient µt using optical coherence tomography. The resulting values of the local total attenuation coefficient µt (mean ± standard error) from necrotic lesions in the dystrophic skeletal muscle tissue of mdx mice are higher (9.6 ± 0.3 mm(-1)) than regions from the same tissue containing only necrotic myofibers (7.0 ± 0.6 mm(-1)), and significantly higher than values from intact myofibers, whether from an adjacent region of the same sample (4.8 ± 0.3 mm(-1)) or from healthy tissue of the wild-type C57 mouse (3.9 ± 0.2 mm(-1)) used as a control. Our results suggest that the attenuation coefficient could be used as a quantitative means to identify necrotic lesions and assess skeletal muscle tissue in mouse models of human Duchenne muscular dystrophy.
Optical + Biomedical Engineering Laboratory, School of Electrical, Electronic and Computer Engineering, The University of Western Australia, M018, 35 Stirling Highway, Crawley, Western Australia 6009, Australia.
Blake R
BR
Klyen
Optical + Biomedical Engineering Laboratory, School of Electrical, Electronic and Computer Engineering, The University of Western Australia, M018, 35 Stirling Highway, Crawley, Western Australia 6009, Australia.
Loretta
L
Scolaro
Skeletal Muscle Research Group, School of Anatomy, Physiology and Human Biology, The University of Western Australia, M309, 35 Stirling Highway, Crawley, Western Australia 6009, Australia.
Tea
T
Shavlakadze
Skeletal Muscle Research Group, School of Anatomy, Physiology and Human Biology, The University of Western Australia, M309, 35 Stirling Highway, Crawley, Western Australia 6009, Australia.
Miranda D
MD
Grounds
Optical + Biomedical Engineering Laboratory, School of Electrical, Electronic and Computer Engineering, The University of Western Australia, M018, 35 Stirling Highway, Crawley, Western Australia 6009, Australia ; Centre for Microscopy, Characterisation and Analysis, The University of Western Australia, M010, 35 Stirling Highway, Crawley, Western Australia 6009, Australia.
David D
DD
Sampson
(100.2960) Image analysis; (110.4500) Optical coherence tomography; (170.6935) Tissue characterization; (290.1350) Backscattering