The PI3K pathway acting on alternative HIV-1 pre-mRNA splicing.
HIV-1 mediates pro-survival signals and prevents apoptosis via the phosphatidylinositol-3-kinase (PI3K) pathway. This pathway, however, also affects phosphorylation of serine-arginine (SR) proteins, a family of splicing regulatory factors balancing splice site selection. We now show that pharmacologic inhibition of PI3K signalling alters the HIV-1 splicing pattern of both minigene- and provirus-derived mRNAs. This indicates that HIV-1 might also promote PI3K signalling to balance processing of its transcripts by regulating phosphorylation of splicing regulatory proteins.
Institute for Virology, Universitaetsklinikum Duesseldorf, Universitaetsstr. 1, D-40225 Duesseldorf, Germany.|Central Division for Electron Microscopy, University of Wuerzburg, Am Hubland, D-97074 Wuerzburg, Germany.
Frank
F
Hillebrand
Institute for Virology, Universitaetsklinikum Duesseldorf, Universitaetsstr. 1, D-40225 Duesseldorf, Germany.
Steffen
S
Erkelenz
Institute for Virology, Universitaetsklinikum Duesseldorf, Universitaetsstr. 1, D-40225 Duesseldorf, Germany.
Nora
N
Diehl
Institute for Virology, Universitaetsklinikum Duesseldorf, Universitaetsstr. 1, D-40225 Duesseldorf, Germany.
Marek
M
Widera
Department of Dermatology, Universitaetsklinikum Duesseldorf, Moorenstrasse 5, D-40225 Duesseldorf, Germany.
Juliane
J
Noffke
Institute for Virology and Immunobiology, University of Wuerzburg, Versbacher Str. 7, D-97078 Wuerzburg, Germany.
Elita
E
Avota
Institute for Virology and Immunobiology, University of Wuerzburg, Versbacher Str. 7, D-97078 Wuerzburg, Germany.
Sibylle
S
Schneider-Schaulies
Central Division for Electron Microscopy, University of Wuerzburg, Am Hubland, D-97074 Wuerzburg, Germany.
Marie-Christine
MC
Dabauvalle
Institute for Virology, Universitaetsklinikum Duesseldorf, Universitaetsstr. 1, D-40225 Duesseldorf, Germany.
Heiner
H
Schaal
D015967:Gene Expression Regulation, Viral; D015497:HIV-1 / Q000235:genetics*; D054884:Host-Pathogen Interactions; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D012322:RNA Precursors / Q000378:metabolism*; D012326:RNA Splicing
D012322:RNA Precursors; D019869:Phosphatidylinositol 3-Kinases