Antioxidant Capacity and Proanthocyanidin Composition of the Bark of Metasequoia glyptostroboides.
Metasequoia glyptostroboides Hu et Cheng is the only living species in the genus Metasequoia Miki ex Hu et Cheng (Taxodiaceae), which is well known as a "living fossil" species. In the Chinese folk medicine, the leaves and bark of M. glyptostroboides are used as antimicrobic, analgesic, and anti-inflammatory drug for dermatic diseases. This study is the first to report the free radical scavenging capacity, antioxidant activity, and proanthocyanidin composition of the bark of M. glyptostroboides. We observed total of six extracts and fractions, which were easily obtained by water-ethanol extraction and followed by a further separation with D101 resin column chromatography, had significant DPPH radical, superoxide anion radical, and hydroxyl radical scavenging capacity, total antioxidative capacity (T-AOC), lipid peroxidation inhibitory activity, and metal ions chelating capacity. The fraction MGEB, which was obtained by 60% ethanol extraction and followed by a further separation with D101 resin column chromatograph, possessed the highest proanthocyanidin content and the highest free radical scavenging and antioxidant activities. Furthermore, MGEB could significantly protect against CCl4 induced acute liver injury through inhibition of oxidative stress in mice. In addition, ten proanthocyanidins were isolated from MGEB, and six of them were firstly reported from this plant.
Institute of Materia Medica, Zhejiang Academy of Medical Sciences, Tianmushan Road 182, Hangzhou, Zhejiang 310013, China.
Fengyang
F
Chen
Institute of Materia Medica, Zhejiang Academy of Medical Sciences, Tianmushan Road 182, Hangzhou, Zhejiang 310013, China.
Lin
L
Zhang
Institute of Materia Medica, Zhejiang Academy of Medical Sciences, Tianmushan Road 182, Hangzhou, Zhejiang 310013, China.
Shuling
S
Zong
Institute of Materia Medica, Zhejiang Academy of Medical Sciences, Tianmushan Road 182, Hangzhou, Zhejiang 310013, China.
Shifang
S
Xu
Institute of Materia Medica, Zhejiang Academy of Medical Sciences, Tianmushan Road 182, Hangzhou, Zhejiang 310013, China.
Xiaoyu
X
Li
Institute of Materia Medica, Zhejiang Academy of Medical Sciences, Tianmushan Road 182, Hangzhou, Zhejiang 310013, China.
Yiping
Y
Ye