Osteochondral regeneration using an oriented nanofiber yarn-collagen type I/hyaluronate hybrid/TCP biphasic scaffold.
Osteochondral defects affect both the articular cartilage and the underlying subchondral bone, but poor osteochondral regeneration is still a daunting challenge. Although the tissue engineering technology provides a promising approach for osteochondral repair, an ideal biphasic scaffold is in high demand with regards to proper biomechanical strength. In this study, an oriented poly(l-lacticacid)-co-poly(ε-caprolactone) P(LLA-CL)/collagen type I(Col-I) nanofiber yarn mesh, fabricated by dynamic liquid electrospinning served as a skeleton for a freeze-dried Col-I/Hhyaluronate (HA) chondral phase (SPONGE) to enhance the mechanical strength of the scaffold. In vitro results show that the Yarn Col-I/HA hybrid scaffold (Yarn-CH) can allow the cell infiltration like sponge scaffolds. Using porous beta-tricalcium phosphate (TCP) as the osseous phase, the Yarn-CH/TCP biphasic scaffold was then assembled by freeze drying. After combination of bone marrow mesenchymal stem cells, the biphasic complex was successfully used to repair the osteochondral defects in a rabbit model with greatly improved repairing scores and compressive modulus.
Department of Orthopaedics, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, 600 Yishan Road, Shanghai, 200233, People's Republic of China.
Shen
S
Liu
Jinglei
J
Wu
Xudong
X
Liu
Desheng
D
Chen
Gary L
GL
Bowlin
Lei
L
Cao
Jianxi
J
Lu
Fengfeng
F
Li
Xiumei
X
Mo
Cunyi
C
Fan
D000818:Animals; D001861:Bone Regeneration; D002130:Calcium Phosphates / Q000737:chemistry*; D024042:Collagen Type I / Q000737:chemistry*; D006820:Hyaluronic Acid / Q000737:chemistry*; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D057139:Nanofibers / Q000737:chemistry*; D016062:Porosity; D011817:Rabbits; D054457:Tissue Scaffolds / Q000737:chemistry*
D002130:Calcium Phosphates; D024042:Collagen Type I; C485817:beta-tricalcium phosphate; D006820:Hyaluronic Acid
biphasic composite; electrospinning; osteochondral defect; yarn