Symbiotic activity of pea (Pisum sativum) after application of Nod factors under field conditions.
Growth and symbiotic activity of legumes are mediated by Nod factors (LCO, lipo-chitooligosaccharides). To assess the effects of application of Nod factors on symbiotic activity and yield of pea, a two-year field experiment was conducted on a Haplic Luvisol developed from loess. Nod factors were isolated from Rhizobium leguminosarum bv. viciae strain GR09. Pea seeds were treated with the Nod factors (10⁻¹¹ M) or water (control) before planting. Symbiotic activity was evaluated by measurements of nitrogenase activity (acetylene reduction assay), nodule number and mass, and top growth by shoot mass, leaf area, and seed and protein yield. Nod factors generally improved pea yield and nitrogenase activity in the relatively dry growing season 2012, but not in the wet growing season in 2013 due to different weather conditions.
Institute of Agrophysics, Polish Academy of Sciences, P.O. Box 201, 20-290 Lublin, Poland. asiczek@ipan.lublin.pl.
Anna
A
Siczek
Institute of Agrophysics, Polish Academy of Sciences, P.O. Box 201, 20-290 Lublin, Poland. j.lipiec@ipan.lublin.pl.
Jerzy
J
Lipiec
Department of Genetics and Microbiology, Maria Curie-Skłodowska University, Akademicka 19 st, 20-033 Lublin, Poland. jerzy.wielbo@poczta.umcs.lublin.pl.
Jerzy
J
Wielbo
Department of Genetics and Microbiology, Maria Curie-Skłodowska University, Akademicka 19 st, 20-033 Lublin, Poland. donia81@gmail.com.
Dominika
D
Kidaj
Institute of Agrophysics, Polish Academy of Sciences, P.O. Box 201, 20-290 Lublin, Poland. pszarlip@ipan.lublin.pl.
Paweł
P
Szarlip
D009591:Nitrogenase / Q000378:metabolism; D009844:Oligosaccharides / Q000302:isolation & purification / Q000378:metabolism*; D018532:Pisum sativum / Q000201:enzymology / Q000254:growth & development / Q000502:physiology*; D016961:Rhizobium leguminosarum / Q000737:chemistry / Q000502:physiology*; D053204:Root Nodules, Plant / Q000201:enzymology / Q000254:growth & development / Q000502:physiology*; D012639:Seeds / Q000254:growth & development / Q000502:physiology*; D013559:Symbiosis
D009844:Oligosaccharides; D009591:Nitrogenase