Arabidopsis miR156 Regulates Tolerance to Recurring Environmental Stress through SPL Transcription Factors.
Plants are sessile organisms that gauge stressful conditions to ensure survival and reproductive success. While plants in nature often encounter chronic or recurring stressful conditions, the strategies to cope with those are poorly understood. Here, we demonstrate the involvement of ARGONAUTE1 and the microRNA pathway in the adaptation to recurring heat stress (HS memory) at the physiological and molecular level. We show that miR156 isoforms are highly induced after HS and are functionally important for HS memory. miR156 promotes sustained expression of HS-responsive genes and is critical only after HS, demonstrating that the effects of modulating miR156 on HS memory do not reflect preexisting developmental alterations. miR156 targets SPL transcription factor genes that are master regulators of developmental transitions. SPL genes are posttranscriptionally downregulated by miR156 after HS, and this is critical for HS memory. Altogether, the miR156-SPL module mediates the response to recurring HS in Arabidopsis thaliana and thus may serve to integrate stress responses with development.
Institute for Biochemistry and Biology, University of Potsdam, 14476 Potsdam, Germany.
Anna
http://orcid.org/0000-0002-6820-8444
A
Stief
Institute for Biochemistry and Biology, University of Potsdam, 14476 Potsdam, Germany.
Simone
S
Altmann
Institute for Biochemistry and Biology, University of Potsdam, 14476 Potsdam, Germany.
Karen
K
Hoffmann
Max-Planck-Institute for Molecular Plant Physiology, 14476 Potsdam, Germany.
Bikram Datt
BD
Pant
Max-Planck-Institute for Molecular Plant Physiology, 14476 Potsdam, Germany.
Wolf-Rüdiger
WR
Scheible
Institute for Biochemistry and Biology, University of Potsdam, 14476 Potsdam, Germany isabel.baeurle@uni-potsdam.de.
Isabel
http://orcid.org/0000-0001-5633-8068
I
Bäurle