Comparative study of tissue reactivity to n-butyl-2-cyanoacrylate and nylon monofilament thread on pericranium-cutaneous flaps in rats.
OBJECTIVE
To study the repair of pericranium-cutaneous flaps fixed with suture anchored in a skull bone tunnel or N-butyl-2-cyanoacrylate adhesive in Wistar rats with emphasis on the cellular inflammatory response and the production of types I and III collagen.


METHODS
The operated region in the cephalic region of Wistar rats was removed minutes before euthanasia, fixed in formalin, and subjected to histological preparation. Slides were stained with hematoxylin-eosin and Picrosirius. Standardized counts of polymorphonuclear and mononuclear cells, fibroblasts, and macrophages were performed, and the percentages of types I and III collagen were determined. Data collection occurred on days 3, 7, 14, 21, and 45 postoperatively. A value of p<0.05 was considered statistically significant.


RESULTS
Quantitative analysis of the data showed more fibroblasts in the surgical adhesive group than in the nylon monofilament thread groups (p=0.0211). Qualitative analysis showed higher reactivity in the adhesive group, with a predominance of polymorphonuclear cells from days 3-45 and macrophages from days 3-7. The amount of type I collagen exceeded 80% in the treated and control groups at the end of the experiment.


CONCLUSIONS
Subperiosteal detachment triggers a cellular inflammatory response that is amplified using soft tissue fixation methods. The adhesive n-butyl-2-cyanoacrylate was more reactive than the nylon monofilament thread anchored in the skull bone tunnel.
Department of Medicine, Center of Health Sciences, State University of Maringa, Maringa, PR, Brazil.
William César
WC
Cavazana
Federal University of Parana, Curitiba, PR, Brazil.
Sergio Osamu
SO
Ioshii
Department of Pharmacology and Therapeutics, Center of Health Sciences, UEM, Maringa, PR, Brazil.
Roberto Kenji
RK
Nakamura Cuman
Department of Surgery, Faculty of Medical Sciences, UNICAMP, Sao Paulo, SP, Brazil.
Luis Augusto
LA
Passeri
D000818:Animals; D003094:Collagen / Q000032:analysis; D004659:Enbucrilate / Q000627:therapeutic use*; D005347:Fibroblasts; D008297:Male; D008422:Materials Testing; D009757:Nylons; D011184:Postoperative Period; D011897:Random Allocation; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012886:Skull / Q000601:surgery*; D013524:Surgical Flaps / Q000473:pathology / Q000601:surgery*; D053441:Suture Anchors; D013536:Suture Techniques; D013997:Time Factors; D016896:Treatment Outcome
D009757:Nylons; D003094:Collagen; D004659:Enbucrilate