The C(RgdyK)-conjugated Fe3O4 nanoparticles with high drug load for dual-targeting integrin alpha(v)beta3-expressing cancer cells.
A novel drug delivery system c(RGDyK)-modified Fe3O4 nanoparticles with high DOX load (R-DMP), which combines magnetic targeting, integrin alpha(v)beta3 targeting and high drug loading properties, was developed by chemical coupling both doxorubicin and peptide c(RGDyK) on the synthetic dual function magnetic nanoparticles (DMP) using a multi-hand cross-linker poly-L-glutamic acid. R-DMP has high drug loading ratio and trapping efficiency for magnetic targeting, and the drug loading ratio can be controlled by adjusting the reactant ratio. Moreover, R-DMP presents narrow size distribution and is sensitive to pH for drug releasing. Compare with those of doxorubicin coupled DMP without peptide c(RGDyK) modification, D-DMP shows enhanced uptake by integrin alpha(v)beta3 targeting expressing tumor cells and displays stronger cancer cell cytotoxicity. This investigate provides a new approach for the dual-targeted delivery of therapeutic agents to tumors with controlled low carrier toxicity and high efficiency.
Lin
L
Guo
Wence
W
Ding
Li-Min
LM
Zheng
D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage / Q000737:chemistry; D045744:Cell Line, Tumor; D004317:Doxorubicin / Q000008:administration & dosage* / Q000737:chemistry; D005910:Glioma / Q000188:drug therapy* / Q000473:pathology; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods / Q000706:statistics & numerical data; D039302:Integrin alphaVbeta3 / Q000378:metabolism*; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D058990:Molecular Targeted Therapy / Q000379:methods*; D053769:Nanocapsules / Q000737:chemistry* / Q000648:ultrastructure; D058726:Nanoconjugates; D010456:Peptides, Cyclic / Q000737:chemistry / Q000493:pharmacokinetics*; D016896:Treatment Outcome
D000903:Antibiotics, Antineoplastic; D039302:Integrin alphaVbeta3; D058185:Magnetite Nanoparticles; D053769:Nanocapsules; D058726:Nanoconjugates; D010456:Peptides, Cyclic; C550210:cyclo(Arg-Gly-Asp-Tyr-Lys); D004317:Doxorubicin