Fermentation supernatants ofLactobacillus delbrueckiiinhibit growth of human colon cancer cells and induce apoptosis through a caspase 3-dependent pathway.
Probiotic bacteria are known to exert a wide range of beneficial effects on their animal hosts. Therefore, the present study explored the effect of the supernatants obtained fromLactobacillus delbrueckiifermentation (LBF) on colon cancer. The results indicated that the proliferation of LBF solution-treated colon cancer SW620 cells was arrested and accumulated in the G1 phase in a concentration-dependent manner. The LBF solution efficiently induced apoptosis through the intrinsic caspase 3-depedent pathway, with a corresponding decreased expression of Bcl-2. The activity of matrix metalloproteinase 9, which is associated with the invasion of colon cancer cells, was also decreased in the LBF-treated cells. In conclusion, the results demonstrate the antitumor effect of LBFin vitroand may contribute to the development of novel therapies for the treatment of colon cancer.
Department of Biotechnology, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.
Ying
Y
Wan
Department of Biotechnology, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.
Yi
Y
Xin
Department of Biotechnology, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.
Cuili
C
Zhang
Department of Biotechnology, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.
Dachang
D
Wu
Clinical Laboratory Department, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116044, P.R. China.
Dapeng
D
Ding
Department of Biotechnology, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.
Li
L
Tang
Department of Biotechnology, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.
Lawrence
L
Owusu
Department of Biotechnology, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.
Jing
J
Bai
Department of Biotechnology, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.
Weiling
W
Li
Lactobacillus delbrueckii; apoptosis; caspase 3; colon cancer; matrix metalloproteinase-9