Amino acid networks in a (β/α)₈ barrel enzyme change during catalytic turnover.
Proteins can be viewed as small-world networks of amino acid residues connected through noncovalent interactions. Nuclear magnetic resonance chemical shift covariance analyses were used to identify long-range amino acid networks in the α subunit of tryptophan synthase both for the resting state (in the absence of substrate and product) and for the working state (during catalytic turnover). The amino acid networks observed stretch from the surface of the protein into the active site and are different between the resting and working states. Modification of surface residues on the network alters the structural dynamics of active-site residues over 25 Å away and leads to changes in catalytic rates. These findings demonstrate that amino acid networks, similar to those studied here, are likely important for coordinating structural changes necessary for enzyme function and regulation.
Department of Chemistry, The Pennsylvania State University , University Park, Pennsylvania 16802, United States.
Jennifer M
JM
Axe
Eric M
EM
Yezdimer
Kathleen F
KF
O'Rourke
Nicole E
NE
Kerstetter
Wanli
W
You
Chia-en A
CE
Chang
David D
DD
Boehr
D000596:Amino Acids / Q000737:chemistry* / Q000378:metabolism; D020134:Catalytic Domain; D007700:Kinetics; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular; D011487:Protein Conformation; D021122:Protein Subunits / Q000737:chemistry / Q000378:metabolism; D012486:Salmonella typhimurium / Q000737:chemistry / Q000201:enzymology*; D014367:Tryptophan Synthase / Q000737:chemistry* / Q000378:metabolism
D000596:Amino Acids; D021122:Protein Subunits; D014367:Tryptophan Synthase