Determination of tetracycline residues in chicken meat by liquid chromatography-tandem mass spectrometry.
Analysis of residual levels of tetracyclines (TCs) in chicken meat was performed using a validated liquid chromatography coupled with a tandem mass spectrometry (LC-MS/MS) technique. Overall, the recoveries for TCs ranged from 56.9% to 101.2%, with standard deviations of 4.5-13.2%. Detection limits ranged from 7.9 to 14.6 µg kg⁻¹. In four of 60 samples, doxycycline (DXC) was determined in a range from 19.9 to 35.6 µg kg⁻¹; and in one sample tetracycline was detected at 17.2 µg kg⁻¹. Chlortetracycline (CTC) and oxytetracycline (OTC) were not detected in any of the tested samples. This study indicates that chicken meat sold in Bursa, Turkey, contained some residues of TCs. Therefore, stricter regulations for the use of antibiotics in the poultry industry and the monitoring of drug residues in chicken meat prior to marketing are needed. Finally, this method has been applied successfully for the confirmation of TCs in chicken meat.
a Faculty of Veterinary Medicine, Department of Food Hygiene and Technology , University of Uludag , 16059 Gorukle Campus , Bursa , Turkey.
F
F
Cetinkaya
A
A
Yibar
G E
GE
Soyutemiz
B
B
Okutan
A
A
Ozcan
M Y
MY
Karaca
D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000737:chemistry; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D004318:Doxycycline / Q000032:analysis / Q000737:chemistry; D004350:Drug Residues / Q000032:analysis*; D005062:European Union; D005506:Food Contamination; D005513:Food Inspection; D019983:Guideline Adherence; D057230:Limit of Detection; D008460:Meat / Q000032:analysis* / Q000191:economics / Q000592:standards; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry; D013752:Tetracycline / Q000032:analysis / Q000737:chemistry; D013754:Tetracyclines / Q000032:analysis* / Q000737:chemistry; D014421:Turkey; D019155:Veterinary Drugs / Q000592:standards
D000900:Anti-Bacterial Agents; D013754:Tetracyclines; D019155:Veterinary Drugs; D013752:Tetracycline; D004318:Doxycycline
meat; veterinary drug residues; veterinary drug residues – antibiotics; veterinary drug residues – chloramphenicol; veterinary drug residues – tetracycline