Development and validation of a sensitive method for simultaneous determination of eight β₂-agonists in pork by ultrasonic-assisted extraction and liquid chromatography/tandem mass spectrometry.
A simple and sensitive ultrasonic-assisted extraction coupled with liquid chromatography-tandem quadrupole mass spectrometry was developed for the simultaneous determination of eight β2-agonists (i.e., phenylethanolamine, fenoterol, formoterol, clenbuterol, ractopamine, salbutamol, terbutaline and tulobuterol) in pork. The recovery rate of ultrasonic-assisted extraction was compared with that of enzymolysis. Results showed that ultrasonic-assisted extraction is a satisfactory method for the treatment of pork samples, yielding β2-agonist recovery rates of 82.0-114.0%. In contrast, most of the recovery rates obtained by enzymolysis were <80%. Linearities obtained ranged from 0.1 to 16.0 μg kg(-1) for terbutaline, formoterol, tulobuterol and phenylethanolamine, 0.2 to 16.0 μg kg(-1) for salbutamol, fenoterol and ractopamine, and 0.2 to 40.0 μg kg(-1) for clenbuterol. The correlation coefficients were >0.9945 for all analytes. The lower limits of quantification were between 0.086 and 0.20 μg kg(-1). The applicability of the proposed method for detecting and quantifying β2-agonists was demonstrated in the analysis of 50 pork samples.
Zhejiang Provincial Key Laboratory of Health Risk Appraisal for Trace Toxic Chemicals, Ningbo Municipal Center for Disease Control and Prevention, Ningbo 315010, China Ningbo Key Laboratory of Poison Research and Control, Ningbo Municipal Center for Disease Control and Prevention, Ningbo 315010, China.
Shengdong
S
Pan
Medical School, Ningbo University, Ningbo 315211, China.
Lixin
L
Zhou
Zhejiang Provincial Key Laboratory of Health Risk Appraisal for Trace Toxic Chemicals, Ningbo Municipal Center for Disease Control and Prevention, Ningbo 315010, China Ningbo Key Laboratory of Poison Research and Control, Ningbo Municipal Center for Disease Control and Prevention, Ningbo 315010, China.
Yonggang
Y
Zhao
Zhejiang Provincial Key Laboratory of Health Risk Appraisal for Trace Toxic Chemicals, Ningbo Municipal Center for Disease Control and Prevention, Ningbo 315010, China Ningbo Key Laboratory of Poison Research and Control, Ningbo Municipal Center for Disease Control and Prevention, Ningbo 315010, China.
Xiaohong
X
Chen
Zhejiang Provincial Key Laboratory of Health Risk Appraisal for Trace Toxic Chemicals, Ningbo Municipal Center for Disease Control and Prevention, Ningbo 315010, China Ningbo Key Laboratory of Poison Research and Control, Ningbo Municipal Center for Disease Control and Prevention, Ningbo 315010, China.
Xiaoping
X
Li
Zhejiang Provincial Key Laboratory of Health Risk Appraisal for Trace Toxic Chemicals, Ningbo Municipal Center for Disease Control and Prevention, Ningbo 315010, China Ningbo Key Laboratory of Poison Research and Control, Ningbo Municipal Center for Disease Control and Prevention, Ningbo 315010, China jmcjc@163.com.
Micong
M
Jin
D000318:Adrenergic beta-Agonists / Q000737:chemistry* / Q000302:isolation & purification*; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D008460:Meat / Q000032:analysis*; D015394:Molecular Structure; D013552:Swine; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014465:Ultrasonics / Q000379:methods*
D000318:Adrenergic beta-Agonists