Wide Range of Biotin (Vitamin H) Content in Foodstuffs and Powdered Milks as Assessed by High-performance Affinity Chromatography.
The biotin (vitamin H) contents of various foodstuffs were determined by using a newly developed high-performance affinity chromatography with a trypsin-treated avidin-bound column. Biotin was derivatized with 9-anthryldiazomethane (ADAM) to fluorescent biotin-ADAM ester. A wide range of biotin contents were found in various foodstuffs depending upon the species (strain), season, organ (of plants and animals), geography, freshness, preparation method and storage method. Among the foodstuffs and fermented foods tested, it was found that wide distributions of biotin content were observed in powdered milk, natto, sake (rice wine), beer, edible oil and sea weed. Since powdered milk is important for child health and development, 14 kinds of powdered and special milks for use in children's diseases were intensively measured. We found that several special milk powders for children with allergies contained low levels of free biotin. Use of these powdered milks caused skin diseases and alopecia in some patients possessing thermolabile serum biotinidase, and administration of free biotin improved their symptoms dramatically. Therefore, it is essential to estimate the total and free biotin contents on each foodstuff in order to improve effective biotin intake and support better health and quality of life for people.
Department of Endocrinology and Metabolism, National Research Institute for Child Health and Development, Tokyo, Japan.
Kou
K
Hayakawa
Department of Endocrinology and Metabolism, National Research Institute for Child Health and Development, Tokyo, Japan.
Noriyuki
N
Katsumata
Department of Endocrinology and Metabolism, National Research Institute for Child Health and Development, Tokyo, Japan.
Kiyomi
K
Abe
Department of Endocrinology and Metabolism, National Research Institute for Child Health and Development, Tokyo, Japan.
Masahiko
M
Hirano
Department of Endocrinology and Metabolism, National Research Institute for Child Health and Development, Tokyo, Japan.
Kazuyuki
K
Yoshikawa
Department of Endocrinology and Metabolism, National Research Institute for Child Health and Development, Tokyo, Japan.
Tsutomu
T
Ogata
Department of Endocrinology and Metabolism, National Research Institute for Child Health and Development, Tokyo, Japan.
Reiko
R
Horikawa
School of Health Science, Gunma University Faculty of Medicine, Maebashi, Japan.
Takeaki
T
Nagamine
foodstuffs; free biotin; powdered milk; total biotin; wide distribution