Point mutations associated with organophosphate and carbamate resistance in Chinese strains of Culex pipiens quinquefasciatus (Diptera: Culicidae).
Acetylcholinesterase resistance has been well documented in many insects, including several mosquito species. We tested the resistance of five wild, Chinese strains of the mosquito Culex pipiens quinquefasciatus to two kinds of pesticides, dichlorvos and propoxur. An acetylcholinesterase gene (ace1) was cloned and sequenced from a pooled sample of mosquitoes from these five strains and the amino acids of five positions were found to vary (V185M, G247S, A328S, A391T, and T682A). Analysis of the correlation between mutation frequencies and resistance levels (LC50) suggests that two point mutations, G247S (r2 = 0.732, P = 0.065) and A328S (r2 = 0.891, P = 0.016), are associated with resistance to propoxur but not to dichlorvos. Although the V185M mutation was not associated with either dichlorvos or propoxur resistance, its RS genotype frequency was correlated with propoxur resistance (r2 = 0.815, P = 0.036). And the HWE test showed the A328S mutation is linked with V185M, also with G247S mutation. This suggested that these three mutations may contribute synergistically to propoxur resistance. The T682A mutation was negatively correlated with propoxur (r2 = 0.788, P = 0.045) resistance. Knowledge of these mutations may help design strategies for managing pesticide resistance in wild mosquito populations.
Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogens and Biosecurity, Department of Vector Biology and Control, Beijing, China; Anhui Medical University, Hefei, China.
Minghui
M
Zhao
Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogens and Biosecurity, Department of Vector Biology and Control, Beijing, China.
Yande
Y
Dong
Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogens and Biosecurity, Department of Vector Biology and Control, Beijing, China; Anhui Medical University, Hefei, China.
Xin
X
Ran
Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogens and Biosecurity, Department of Vector Biology and Control, Beijing, China.
Zhiming
Z
Wu
Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogens and Biosecurity, Department of Vector Biology and Control, Beijing, China.
Xiaoxia
X
Guo
Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogens and Biosecurity, Department of Vector Biology and Control, Beijing, China.
Yingmei
Y
Zhang
Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogens and Biosecurity, Department of Vector Biology and Control, Beijing, China.
Dan
D
Xing
Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogens and Biosecurity, Department of Vector Biology and Control, Beijing, China.
Ting
T
Yan
Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogens and Biosecurity, Department of Vector Biology and Control, Beijing, China.
Gang
G
Wang
Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogens and Biosecurity, Department of Vector Biology and Control, Beijing, China.
Xiaojuan
X
Zhu
Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogens and Biosecurity, Department of Vector Biology and Control, Beijing, China.
Hengduan
H
Zhang
Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogens and Biosecurity, Department of Vector Biology and Control, Beijing, China.
Chunxiao
C
Li
Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogens and Biosecurity, Department of Vector Biology and Control, Beijing, China.
Tongyan
T
Zhao
D000110:Acetylcholinesterase / Q000737:chemistry / Q000235:genetics; D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002219:Carbamates / Q000494:pharmacology*; D020134:Catalytic Domain; D002800:Cholinesterase Inhibitors / Q000494:pharmacology; D003465:Culex / Q000187:drug effects* / Q000235:genetics*; D004006:Dichlorvos / Q000494:pharmacology; D005260:Female; D005787:Gene Frequency; D008040:Genetic Linkage; D007305:Insecticide Resistance / Q000235:genetics*; D015810:Linkage Disequilibrium; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009154:Mutation; D010755:Organophosphates / Q000494:pharmacology*; D017354:Point Mutation; D011110:Polymorphism, Genetic; D001074:Propoxur / Q000494:pharmacology; D011487:Protein Conformation
D002219:Carbamates; D002800:Cholinesterase Inhibitors; D010755:Organophosphates; D004006:Dichlorvos; D001074:Propoxur; D000110:Acetylcholinesterase