Survival of bone marrow mesenchymal stem cells labelled with red fluorescent protein in an ovine model of collagenase-induced tendinitis.
OBJECTIVE
The aim of this study was to track the survival and efficacy of allogeneic bone marrow mesenchymal stem cells (BM-MSC) marked with red fluorescent protein (BM-MSCRFP) in an ovine model of collagenase-induced tendinopathy.


METHODS
Bone marrow was harvested from one donor sheep and BM-MSC were isolated, cultivated and transfected with red fluorescent protein (BM-MSCRFP). Collagenase was injected into both Achilles tendons in the remaining nine sheep. After two weeks the left tendon was injected with a solution of 6 x 10⁶ BM-MSCRFP and fibrin glue, while only fibrin glue was administered to the contra-lateral tendon in each sheep. After three, four and six weeks the tendons were harvested and evaluated for morphology, collagen I deposition, presence of CD34+ cells, and fluorescent labelled BM-MSC.


RESULTS
We demonstrated that delivery of BM-MSC into tendon lesions had positive effects on the injured tendons. The BM-MSCRFP survived at three, four and six weeks after treatment, leading to better quality healing of tendons as compared to the controls, where no labelled cells were detected. Interestingly, we demonstrated high expression of CD34+ cells in tendons that had been treated with BM-MSCRFP.


CONCLUSIONS
Mesenchymal stem cell allografts have a positive effect on tendon healing and local injection of BM-MSC directly into the tendon allows the homing of BM-MSC for good efficiency of engraftment.
Luca Lacitignola, DVM, Ph.D., Sezione di Cliniche Veterinarie e P.A, Università degli Studi di Bari "Aldo Moro", s.p. per Casamassima Km 3, 70010, Valenzano, BARI, Italy, E-mail: luca.lacitignola@uniba.it.
L
L
Lacitignola
F
F
Staffieri
G
G
Rossi
E
E
Francioso
A
A
Crovace
D000818:Animals; D001854:Bone Marrow Cells / Q000502:physiology*; D005260:Female; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism*; D045164:Mesenchymal Stem Cell Transplantation / Q000662:veterinary*; D012756:Sheep; D012757:Sheep Diseases / Q000139:chemically induced* / Q000628:therapy; D052256:Tendinopathy / Q000139:chemically induced*; D000097573:Red Fluorescent Protein
D008164:Luminescent Proteins; D000097573:Red Fluorescent Protein
Achilles tendon; BM-MSC; CD34+ cells; collagen I; red fluorescent protein; tendinopathy