Essential oils with insecticidal activity against larvae of Aedes aegypti (Diptera: Culicidae).
Insecticidal activity of the essential oils (EOs) isolated from Tagetes lucida, Lippia alba, Lippia origanoides, Eucalyptus citriodora, Cymbopogon citratus, Cymbopogon flexuosus, Citrus sinensis, Swinglea glutinosa, and Cananga odorata aromatic plants, grown in Colombia (Bucaramanga, Santander), and of a mixture of L. alba and L. origanoides EOs were evaluated on Aedes (Stegomyia) aegypti Rockefeller larvae. The EOs were extracted by microwave-assisted hydrodistillation and characterized by gas chromatography-mass spectrometry (GC-MS). The main components of the EOs were identified using their linear retention indices and mass spectra. The lethal concentrations (LCs) of the EOs were determined between the third and fourth instar of A. aegypti. LC50 was determined by probit analysis using mortality rates of bioassays. All essential oils tested showed insecticidal activity. The following values were obtained for C. flexuosus (LC50 = 17.1 ppm); C. sinensis (LC50 = 20.6 ppm); the mixture of L. alba and L. origanoides (LC50 = 40.1 ppm); L. alba (LC50 = 42.2 ppm); C. odorata (LC50 = 52.9 ppm); L. origanoides (LC50 = 53.3 ppm); S. glutinosa (LC50 = 65.7 ppm); T. lucida (LC50 = 66.2 ppm); E. citriodora (LC50 = 71.2 ppm); and C. citratus (LC50 = 123.3 ppm). The EO from C. flexuosus, with citral (geranial + neral) as main component, showed the highest larvicidal activity.
Center for Research on Tropical Diseases (Centro de Investigación en Enfermedades Tropicales-CINTROP), Faculty of Health, School of Medicine, Department of Basic Sciences, Universidad Industrial de Santander, Bucaramanga, Colombia.
Sharon Smith
SS
Vera
Diego Fernando
DF
Zambrano
Stelia Carolina
SC
Méndez-Sanchez
Fernando
F
Rodríguez-Sanabria
Elena E
EE
Stashenko
Jonny E
JE
Duque Luna
D000330:Aedes; D000818:Animals; D032084:Citrus sinensis / Q000737:chemistry; D003105:Colombia; D031743:Cymbopogon / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D020128:Inhibitory Concentration 50; D007306:Insecticides / Q000302:isolation & purification*; D007814:Larva; D032411:Lippia / Q000737:chemistry; D009822:Oils, Volatile / Q000302:isolation & purification*; D027821:Rutaceae / Q000737:chemistry; D031686:Tagetes / Q000737:chemistry
D007306:Insecticides; D009822:Oils, Volatile