Turkey versus guinea pig red blood cells: hemagglutination differences alter hemagglutination inhibition responses against influenza A/H1N1.
For influenza hemagglutination inhibition (HAI) assays, species selection of red blood cells (RBCs) is critical to determine antibody titers to influenza viruses reliably. We compared pandemic influenza virus A/H1N1 (pdH1N1) HAI titers using turkey or guinea pig RBCs. Turkey RBCs appear to be the more appropriate species choice for influenza A/pH1N1 HAI assays.
1 Mayo Clinic Vaccine Research Group, Mayo Clinic , Rochester, Minnesota.
Inna G
IG
Ovsyannikova
Sarah J
SJ
White
Randy A
RA
Albrecht
Adolfo
A
García-Sastre
Gregory A
GA
Poland
D000368:Aged; D000818:Animals; D004912:Erythrocytes / Q000821:virology*; D006168:Guinea Pigs; D006385:Hemagglutination Inhibition Tests / Q000379:methods*; D006387:Hemagglutination, Viral; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000276:immunology*; D008875:Middle Aged; D014422:Turkeys