Targeted efficacy of dihydroartemisinin for translationally controlled protein expression in a lung cancer model.
OBJECTIVE
Lung cancer is one of the malignant tumors with greatest morbidity and mortality around the world. The keys to targeted therapy are discovery of lung cancer biomarkers to facilitate improvement of survival and quality of life for the patients with lung cancer. Translationally controlled tumor protein (TCTP) is one of the most overexpressed proteins in human lung cancer cells by comparison to the normal cells, suggesting that it might be a good biomarker for lung cancer.


METHODS
In the present study, the targeted efficacy of dihydroartemisinin (DHA) on TCTP expression in the A549 lung cancer cell model was explored.


CONCLUSIONS
DHA could inhibit A549 lung cancer cell proliferation, and simultaneously up-regulate the expression of TCTP mRNA, but down-regulate its protein expression in A549 cells. In addition, it promoted TCTP protein secretion. Therefore, TCTP might be used as a potential biomarker and therapeutic target for non-small cell lung cancers.
Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China E-mail : jinanzh506nj@sina.com.
Lian-Ke
LK
Liu
Heng-Fang
HF
Wu
Zhi-Rui
ZR
Guo
Xiang-Jian
XJ
Chen
Di
D
Yang
Yong-Qian
YQ
Shu
Ji-Nan
JN
Zhang
D000962:Antimalarials / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D037621:Artemisinins / Q000494:pharmacology*; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D049109:Cell Proliferation / Q000187:drug effects*; D004273:DNA, Neoplasm / Q000235:genetics; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000378:metabolism* / Q000473:pathology; D060888:Real-Time Polymerase Chain Reaction; D014407:Tumor Cells, Cultured; D000090324:Tumor Protein, Translationally-Controlled 1
D000962:Antimalarials; D037621:Artemisinins; D014408:Biomarkers, Tumor; D004273:DNA, Neoplasm; C000715647:TPT1 protein, human; D000090324:Tumor Protein, Translationally-Controlled 1; C039060:artenimol