Effect of protein synthesis rhythm-organizing signal persists for a day after single administration of melatonin to rat.
Melatonin administered to rat intraperitoneally organizes ultradian rhythm of protein synthesis in hepatocytes that persists for 1 day after exposure to the synchronizing signal. Hepatocytes were isolated 1 day after melatonin administration and cultured on coverslips in a serum-free medium. In 24 h in culture, the kinetics of protein synthesis was analyzed. In our previous experiments, we detected a rhythm in cells isolated in 1.5 h, but not in 3 days after melatonin administration to the rat. We have found that synchronization of oscillations of the protein synthesis intensity in vivo persists over 1 day after rat exposure to melatonin. Phenylephrine, an efficient synchronizer of protein synthesis in vitro, does not organize the rhythm in vivo.
N.K. Kol'tsov Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia.
N D
ND
Zvezdina
L A
LA
Malchenko
D S
DS
Konchenko
T K
TK
Dubovaya
V Ya
VY
Brodskii
D000818:Animals; D008550:Melatonin / Q000008:administration & dosage* / Q000494:pharmacology; D014176:Protein Biosynthesis / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar
D008550:Melatonin