Comparison and evaluation of immobilization methods for preparing bacterial probes using acidophilic bioleaching bacteria Acidithiobacillus thiooxidans for AFM studies.
We evaluated different strategies for constructing bacterial probes for atomic force microscopy studies of bioleaching Acidithiobacillus thiooxidans interacting with pyrite mineral surfaces. Of three available techniques, the bacterial colloidal probe technique is the most reliable and provides a versatile platform for quantifying true interactive forces between bioleaching microorganisms and mineral surfaces.
School of Chemical Engineering, The University of Queensland, St Lucia, QLD 4072, Australia.
Mengxue
M
Diao
Australian Institute for Bioengineering and Nanotechnology, ANFF-Q, The University of Queensland, Brisbane, QLD 4072, Australia.
Elena
E
Taran
School of Chemical Engineering, The University of Queensland, St Lucia, QLD 4072, Australia; Australian Institute for Bioengineering and Nanotechnology, ANFF-Q, The University of Queensland, Brisbane, QLD 4072, Australia.
Stephen M
SM
Mahler
School of Chemical Engineering, The University of Queensland, St Lucia, QLD 4072, Australia. Electronic address: anh.nguyen@eng.uq.edu.au.
Anh V
AV
Nguyen
D013856:Acidithiobacillus thiooxidans / Q000502:physiology* / Q000648:ultrastructure*; D001422:Bacterial Adhesion; D008828:Microbiological Techniques / Q000379:methods*; D018625:Microscopy, Atomic Force / Q000379:methods*; D008903:Minerals / Q000378:metabolism; D013499:Surface Properties
D008903:Minerals
Acidithiobacillus thiooxidans; Adhesion; Atomic force microscopy; Bacterial probe; Immobilization; Pyrite