Application of a capillary-assembled microfluidic system for separation of cephalosporins.
This paper demonstrates a simple and easy setting up of a fused-silica capillary-assembled microfluidic system (μCE). This system incorporates a split-flow pressure injection of the sample into a microfluidic system made from PDMS and a short (∼20 cm) length of fused-silica capillary as a separation unit. The on-capillary detection was carried out by fiber optic spectrometry. A mixture of six cephalosporin antibiotics was separated in the μCE system and the obtained results were compared to those achievable by conventional CE. The six components could be separated within 8.5 min with the number of theoretical plates around 10 000.
Department of Inorganic and Analytical Chemistry, University of Debrecen, Debrecen, Hungary.
Peter I
PI
Koczka
Attila
A
Gaspar
D002511:Cephalosporins / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification*; D019075:Electrophoresis, Capillary / Q000295:instrumentation*; D004867:Equipment Design; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D015203:Reproducibility of Results
D002511:Cephalosporins
CE; Microchip; Poly(dimethylsiloxane)