β-actin in the signaling of transmembrane TNF-α-mediated cytotoxicity.
To study the role of β-actin in the signaling of transmembrane TNF-α (tmTNF-α)-mediated cytotoxicity, we mainly used bioassay and apoptosis assay, detection of the visual changes in β-actin and intracellular translocation of signal molecules in response to tmTNF-α, and analysis of the signal molecules coupled or uncoupled with TNFR2 complex. These protocols might also be used to investigate the signaling events mediated by other transmembrane cytokines or members of TNF superfamily, which have transmembrane and soluble forms, as well as the involvement of actin cytoskeleton in signal transduction pathways.
Department of Immunology, Medical College, Wuhan University of Science and Technology, Wuhan, Hubei, PR China.
Hui
H
Chen
Yan
Y
Leng
Zhuoya
Z
Li
D000199:Actins / Q000378:metabolism*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D002454:Cell Differentiation / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects* / Q000378:metabolism; D002467:Cell Nucleus / Q000187:drug effects* / Q000378:metabolism; D003593:Cytoplasm / Q000187:drug effects* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D047468:Immunoprecipitation; D051379:Mice; D018613:Microscopy, Confocal; D016328:NF-kappa B / Q000378:metabolism; D041681:NIH 3T3 Cells; D047888:Receptors, Tumor Necrosis Factor, Type I / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D000199:Actins; D016328:NF-kappa B; D047888:Receptors, Tumor Necrosis Factor, Type I; D014409:Tumor Necrosis Factor-alpha