Electrochemical immunosensor for detecting the spore wall protein of Nosema bombycis based on the amplification of hemin/G-quadruplex DNAzyme concatamers functionalized Pt@Pd nanowires.
In this work, an ultrasensitive electrochemical immunosensor for detecting the Pebrine disease related spore wall protein of Nosema bombycis (SWP N.b) was fabricated based on the amplification of hemin/G-quadruplex functionalized Pt@Pd nanowires (Pt@PdNWs). The synthesized Pt@PdNWs possessed large surface area, which could effectively improve the immobilization amount of hemin/G-quadruplex DNAzyme concatamers produced via hybridization chain reaction (HCR). In the presence of SWP N.b, the hemin/G-quadruplex labeled Pt@PdNWs bioconjugations was captured on electrode surface and thus obtained electrochemical signal. After the addition of NADH into the electrolytic cell, hemin/G-quadruplex firstly acted as an NADH oxidase to locally produce H2O2 in the presence of dissolved O2. Then, the generated H2O2 would be quickly reduced via hemin/G-quadruplex as a horseradish peroxidase mimicking (HRP-mimicking) DNAzyme, which finally promoted the self-redox reaction of hemin/G-quadruplex and a greatly enhanced electrochemical signal was obtained. Furthermore, Pt@PdNWs with excellent electrocatalytic performance could also amplify electrochemical signal. With these amplification factors, the electrochemical immunosensor exhibited a wide linear range from 0.001 ng mL(-1) to 100 ng mL(-1) with a detection limit (LOD) of 0.24 pg mL(-1), providing a new promise for the diagnosis of Pebrine disease.
State Key Laboratory of Silkworm Genome Biology, Key Laboratory of Luminescent and Real-Time Analytical Chemistry, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
Qin
Q
Wang
State Key Laboratory of Silkworm Genome Biology, Key Laboratory of Luminescent and Real-Time Analytical Chemistry, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
Yue
Y
Song
State Key Laboratory of Silkworm Genome Biology, Key Laboratory of Luminescent and Real-Time Analytical Chemistry, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
Yaqin
Y
Chai
State Key Laboratory of Silkworm Genome Biology, Key Laboratory of Luminescent and Real-Time Analytical Chemistry, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
Guoqing
G
Pan
State Key Laboratory of Silkworm Genome Biology, Key Laboratory of Luminescent and Real-Time Analytical Chemistry, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
Tian
T
Li
State Key Laboratory of Silkworm Genome Biology, Key Laboratory of Luminescent and Real-Time Analytical Chemistry, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China. Electronic address: y198688@swu.edu.cn.
Yali
Y
Yuan
State Key Laboratory of Silkworm Genome Biology, Key Laboratory of Luminescent and Real-Time Analytical Chemistry, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China. Electronic address: yuanruo@swu.edu.cn.
Ruo
R
Yuan
D015374:Biosensing Techniques / Q000295:instrumentation; D003217:Conductometry / Q000295:instrumentation*; D021881:DNA, Catalytic / Q000235:genetics*; D044708:DNA, Concatenated / Q000235:genetics; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005656:Fungal Proteins / Q000032:analysis* / Q000235:genetics; D054856:G-Quadruplexes; D006427:Hemin / Q000235:genetics*; D007118:Immunoassay / Q000295:instrumentation*; D053770:Nanowires / Q000737:chemistry / Q000648:ultrastructure; D016818:Nosema / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D021141:Nucleic Acid Amplification Techniques / Q000295:instrumentation; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation; D010165:Palladium / Q000737:chemistry; D010984:Platinum / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013172:Spores, Fungal / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism
D021881:DNA, Catalytic; D044708:DNA, Concatenated; D005656:Fungal Proteins; D010984:Platinum; D010165:Palladium; D006427:Hemin
Electrocatalytic; Electrochemical immunosensor; Hemin/G-quadruplex; Nosema bombycis; Pebrine disease; Pt@Pb nanowires