Bioaccessibility, in vitro antioxidant activities and in vivo anti-inflammatory activities of a purple tomato (Solanum lycopersicum L.).
The bioaccessibility, antioxidant activities and anti-inflammatory activities of phytochemicals in a purple tomato (Solanum lycopersicum L.) V118 was studied using a simulated gastrointestinal digestion model, chemical and cell based antioxidant assays. The total phenolic and carotenoid contents and the antioxidant activities were significantly lowered (37-72%) and degradation seemed to have occurred during the in vitro digestion. Results indicated that these phytochemicals were bioavailable to the cells as demonstrated by the cell based antioxidant assay. Extracts from the purple tomato showed significant and dose dependent anti-inflammatory effect in the in vivo carrageenan-induced paw oedema rat study (oedematous inhibition: 7.48% and 13.8%), suggesting that anthocyanins may play a role in the anti-inflammatory effect. Direct antioxidant actions as indicated by reduced MDA and NO production and indirect actions as shown in increased GPx and SOD activities in oedematous tissue support the conclusion that tomatoes containing anthocyanins can potentially provide better protection against oxidative stress related chronic diseases of humans.
State Key Lab of Food Science and Technology, Institute for Advanced Study, Nanchang University, Nanchang 330047, Jiangxi, China; Guelph Food Research Centre, Agriculture and Agri-Food Canada, 93 Stone Road West, Guelph, Ontario N1G 5C9, Canada.
Hongyan
H
Li
State Key Lab of Food Science and Technology, Institute for Advanced Study, Nanchang University, Nanchang 330047, Jiangxi, China. Electronic address: zeyuandeng@hotmail.com.
Zeyuan
Z
Deng
Guelph Food Research Centre, Agriculture and Agri-Food Canada, 93 Stone Road West, Guelph, Ontario N1G 5C9, Canada.
Ronghua
R
Liu
University of Guelph Ridgetown Campus, 120 Main Street East, Ridgetown, Ontario N0P 2C0, Canada.
Steven
S
Loewen
Guelph Food Research Centre, Agriculture and Agri-Food Canada, 93 Stone Road West, Guelph, Ontario N1G 5C9, Canada. Electronic address: Rong.Cao@agr.gc.ca.
Rong
R
Tsao
D000818:Animals; D000872:Anthocyanins / Q000008:administration & dosage / Q000737:chemistry; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology*; D000975:Antioxidants / Q000008:administration & dosage* / Q000737:chemistry; D003116:Color; D004487:Edema / Q000188:drug therapy* / Q000378:metabolism; D006801:Humans; D018551:Solanum lycopersicum / Q000737:chemistry*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar
D000872:Anthocyanins; D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D010936:Plant Extracts
Anthocyanins; Antioxidant activity; Bioaccessibility; Carrageenan-induced paw oedema; Gastrointestinal digestion; Purple tomato