Antialcoholic liver activity of whey fermented by Lactobacillus casei isolated from koumiss.
Whey fermented liquid (WFL) was studied for its hepatoprotective effects by using chronic alcohol-induced mice. Whey fermented liquid, prepared by inoculating whey with 4% (vol/vol) Lactobacillus casei and then incubating at 41°C for 8h, was used to orally treat alcohol-induced mice at 3 dosages for 5 wk. Ethanol consumption significantly reduced the activity of superoxide dismutase and glutathione peroxidase, while lowering glutathione content and increasing levels of aspartate aminotransferase, alanine aminotransferase, total triglyceride, malondialdehyde, and cytochrome P450 2E1. Treatment with WFL significantly attenuated the increased levels of alanine aminotransferase, aspartate aminotransferase, triglyceride, and cytochrome P450 2E1, while decreasing superoxide dismutase, glutathione peroxidase, malondialdehyde, and glutathione levels. Pathological changes in the livers of mice who had ingested alcohol were improved by the administration of WFL. These results suggest that WFL may exert a protective effect against alcoholic liver disease by increasing antioxidant activity, which supports the use of WFL as an antialcoholic liver disease treatment.
Food Science and Technology Department of the Marine Science School, Ningbo University, Ningbo 315211, P. R. China.
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Zhao
Food Science and Technology Department of the Marine Science School, Ningbo University, Ningbo 315211, P. R. China. Electronic address: daodongpan@163.com.
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Pan
Food Science and Nutrition Department, Nanjing Normal University, Nanjing 210097, P. R. China.
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Wu
Food Science and Technology Department of the Marine Science School, Ningbo University, Ningbo 315211, P. R. China.
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Sun
Food Science and Nutrition Department, Nanjing Normal University, Nanjing 210097, P. R. China.
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Guo
Food Science and Technology Department of the Marine Science School, Ningbo University, Ningbo 315211, P. R. China.
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Zeng
D000818:Animals; D002417:Cattle; D043302:Cultured Milk Products / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005285:Fermentation; D007780:Lacticaseibacillus casei / Q000737:chemistry* / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D008099:Liver / Q000201:enzymology; D008108:Liver Diseases, Alcoholic / Q000188:drug therapy*; D051379:Mice; D012336:RNA, Ribosomal, 16S / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D000067796:Whey / Q000008:administration & dosage / Q000378:metabolism*
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S
Lactobacillus casei; alcoholic liver; antioxidation; fermented whey