Protective Immune Response against Bacillus anthracis Induced by Intranasal Introduction of a Recombinant Adenovirus Expressing the Protective Antigen Fused to the Fc-fragment of IgG2a.
Anthrax is a particularly dangerous infectious disease that affects humans and livestock. It is characterized by intoxication, serosanguineous skin lesions, development of lymph nodes and internal organs, and may manifest itsself in either a cutaneous or septic form. The pathogenic agent is Bacillus anthracis, a grampositive, endospore-forming, rod-shaped aerobic bacterium. Efficacious vaccines that can rapidly induce a long-term immune response are required to prevent anthrax infection in humans. In this study, we designed three recombinant human adenovirus serotype-5-based vectors containing various modifications of the fourth domain of the B. anthracis protective antigen (PA). Three PA modifications were constructed: a secretable form (Ad-sPA), a non-secretable form (Ad-cPA), and a form with the protective antigen fused to the Fc fragment of immunoglobulin G2a (Ad-PA-Fc). All these forms exhibited protective properties against Bacillus anthracis. The highest level of protection was induced by the Ad-PA-Fc recombinant adenovirus. Our findings indicate that the introduction of the Fc antibody fragment into the protective antigen significantly improves the protective properties of the Ad-PA-Fc adenovirus against B. anthracis.
Gamaleya Research Institute for Epidemiology and Microbiology, Ministry of Public Health of the Russian Federation, Gamaleya Street 18, 123098, Moscow, Russia.
D N
DN
Shcherbinin
Gamaleya Research Institute for Epidemiology and Microbiology, Ministry of Public Health of the Russian Federation, Gamaleya Street 18, 123098, Moscow, Russia.
I B
IB
Esmagambetov
Gamaleya Research Institute for Epidemiology and Microbiology, Ministry of Public Health of the Russian Federation, Gamaleya Street 18, 123098, Moscow, Russia.
A N
AN
Noskov
National Research Institute for Veterinary Virology and Microbiology of Russia, Russian Academy of Agricultural Sciences, 601120, Pokrov, Vladimir region, Russia.
Yu O
YO
Selyaninov
Gamaleya Research Institute for Epidemiology and Microbiology, Ministry of Public Health of the Russian Federation, Gamaleya Street 18, 123098, Moscow, Russia.
I L
IL
Tutykhina
Gamaleya Research Institute for Epidemiology and Microbiology, Ministry of Public Health of the Russian Federation, Gamaleya Street 18, 123098, Moscow, Russia.
M M
MM
Shmarov
Gamaleya Research Institute for Epidemiology and Microbiology, Ministry of Public Health of the Russian Federation, Gamaleya Street 18, 123098, Moscow, Russia.
D Yu
DY
Logunov
Gamaleya Research Institute for Epidemiology and Microbiology, Ministry of Public Health of the Russian Federation, Gamaleya Street 18, 123098, Moscow, Russia.
B S
BS
Naroditskiy
Gamaleya Research Institute for Epidemiology and Microbiology, Ministry of Public Health of the Russian Federation, Gamaleya Street 18, 123098, Moscow, Russia.
A L
AL
Gintsburg
Bacillus anthracis; immunization; protective antigen; recombinant adenovirus