Hydrogen sulfide attenuates the recruitment of CD11b⁺Gr-1⁺ myeloid cells and regulates Bax/Bcl-2 signaling in myocardial ischemia injury.
Hydrogen sulfide, an endogenous signaling molecule, plays an important role in the physiology and pathophysiology of the cardiovascular system. Using a mouse model of myocardial infarction, we investigated the anti-inflammatory and anti-apoptotic effects of the H2S donor sodium hydrosulfide (NaHS). The results demonstrated that the administration of NaHS improved survival, preserved left ventricular function, limited infarct size, and improved H2S levels in cardiac tissue to attenuate the recruitment of CD11b(+)Gr-1(+) myeloid cells and to regulate the Bax/Bcl-2 pathway. Furthermore, the cardioprotective effects of NaHS were enhanced by inhibiting the migration of CD11b(+)Gr-1(+) myeloid cells from the spleen into the blood and by attenuating post-infarction inflammation. These observations suggest that the novel mechanism underlying the cardioprotective function of H2S is secondary to a combination of attenuation the recruitment of CD11b(+)Gr-1(+) myeloid cells and regulation of the Bax/Bcl-2 apoptotic signaling.
1] Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai, 200032, China [2].
Youen
Y
Zhang
1] Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai, 200032, China [2] Departments of Physiology and Medicine/CVRL, UCLA School of Medicine, Los Angeles, California, 90095, USA [3].
Hua
H
Li
Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai, 200032, China.
Gang
G
Zhao
1] Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai, 200032, China [2] Institutes of Biomedical Sciences, Fudan University, Shanghai, 200032, China.
Aijun
A
Sun
1] Departments of Physiology and Medicine/CVRL, UCLA School of Medicine, Los Angeles, California, 90095, USA [2] NHLBI Proteomics Center at UCLA/NHLBI Proteomics Program, UCLA School of Medicine, Los Angeles, California, 90095, USA.
Nobel C
NC
Zong
Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai, 200032, China.
Zhaofeng
Z
Li
Departments of Physiology and Medicine/CVRL, UCLA School of Medicine, Los Angeles, California, 90095, USA.
Hongming
H
Zhu
1] Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai, 200032, China [2] Institutes of Biomedical Sciences, Fudan University, Shanghai, 200032, China.
Yunzeng
Y
Zou
Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai, 200032, China.
Xiangdong
X
Yang
1] Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai, 200032, China [2] Institutes of Biomedical Sciences, Fudan University, Shanghai, 200032, China.
Junbo
J
Ge
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D039481:CD11b Antigen / Q000378:metabolism; D002465:Cell Movement / Q000187:drug effects; D004195:Disease Models, Animal; D006352:Heart Ventricles / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D006862:Hydrogen Sulfide / Q000008:administration & dosage / Q000494:pharmacology*; D007249:Inflammation / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D022423:Myeloid Cells / Q000187:drug effects* / Q000378:metabolism* / Q000473:pathology; D009203:Myocardial Infarction / Q000188:drug therapy / Q000378:metabolism / Q000401:mortality / Q000473:pathology; D017202:Myocardial Ischemia / Q000188:drug therapy / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D032383:Myocytes, Cardiac / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D019707:Receptors, Chemokine / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects*; D016277:Ventricular Function, Left / Q000187:drug effects; D051028:bcl-2-Associated X Protein / Q000378:metabolism*
D000893:Anti-Inflammatory Agents; D039481:CD11b Antigen; C503133:Gr-1 protein, mouse; D019253:Proto-Oncogene Proteins c-bcl-2; D019707:Receptors, Chemokine; D051028:bcl-2-Associated X Protein; D006862:Hydrogen Sulfide