Adjuvant effects of chitosan and calcium phosphate particles in an inactivated Newcastle disease vaccine.
The adjuvant activity of chitosan (CS) and calcium phosphate (CAP) particles was studied following intranasal (mucosal) administration to commercial chickens with inactivated Newcastle disease virus (NDV) vaccine. After three vaccinations with inactivated NDV in combination with CS or CAP an increase in antibody titers in blood and mucosal samples in chickens was observed when compared with the administration of NDV antigen only. A lower level of humoral immunity was observed in broiler chickens compared to layer-type birds. The CS-based vaccine demonstrated higher antigenic and protective activity following lethal challenge than the vaccine containing CAP. Because CS particles efficiently changed mucosal and humoral immunity and protective activity, CS may in the future be considered for use as a potential adjuvant for production of vaccines for poultry.
Marina A
MA
Volkova
Anna V
AV
Irza
Irina A
IA
Chvala
Sergy F
SF
Frolov
Vladimir V
VV
Drygin
Darrell R
DR
Kapczynski
D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000378:metabolism*; D000281:Administration, Intranasal / Q000662:veterinary; D000818:Animals; D002130:Calcium Phosphates / Q000008:administration & dosage / Q000378:metabolism; D002645:Chickens; D048271:Chitosan / Q000008:administration & dosage / Q000378:metabolism; D005260:Female; D056724:Immunity, Humoral; D018928:Immunity, Mucosal; D009521:Newcastle Disease / Q000276:immunology* / Q000517:prevention & control / Q000821:virology; D009522:Newcastle disease virus / Q000276:immunology*; D011201:Poultry Diseases / Q000276:immunology* / Q000517:prevention & control / Q000821:virology; D014765:Viral Vaccines / Q000008:administration & dosage*
D000276:Adjuvants, Immunologic; D002130:Calcium Phosphates; D014765:Viral Vaccines; D048271:Chitosan; C020243:calcium phosphate