Quorum sensing activity of Aeromonas caviae strain YL12, a bacterium isolated from compost.
Quorum sensing is a well-studied cell-to-cell communication method that involves a cell-density dependent regulation of genes expression mediated by signalling molecules. In this study, a bacterium isolated from a plant material compost pile was found to possess quorum sensing activity based on bioassay screening. Isolate YL12 was identified using matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry and molecular typing using rpoD gene which identified the isolate as Aeromonas caviae. High resolution tandem mass spectrometry was subsequently employed to identify the N-acyl homoserine lactone profile of Aeromonas caviae YL12 and confirmed that this isolate produced two short chain N-acyl homoserine lactones, namely C4-HSL and C6, and the production was observed to be cell density-dependent. Using the thin layer chromatography (TLC) bioassay, both AHLs were found to activate C. violaceum CV026, whereas only C6-HSL was revealed to induce bioluminescence expression of E. coli [pSB401]. The data presented in this study will be the leading steps in understanding the role of quorum sensing in Aeromonas caviae strain YL12.
Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia. yanluelim@hotmail.my.
Yan-Lue
YL
Lim
Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia. robsonee@live.com.
Robson
R
Ee
Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia. yinwaifong@yahoo.com.
Wai-Fong
WF
Yin
Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia. kokgan@um.edu.my.
Kok-Gan
KG
Chan
D054742:Acyl-Butyrolactones / Q000378:metabolism; D057585:Aeromonas caviae / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology*; D001483:Base Sequence; D015374:Biosensing Techniques; D002853:Chromatography, Liquid; D002855:Chromatography, Thin Layer; D005798:Genes, Bacterial; D010802:Phylogeny; D053038:Quorum Sensing; D012987:Soil; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D054742:Acyl-Butyrolactones; D012987:Soil