Acute ethanol-induced changes in edema and metabolite concentrations in rat brain.
The aim of this study is to describe the acute effects of EtOH on brain edema and cerebral metabolites, using diffusion weight imaging (DWI) and proton magnetic resonance spectroscopy ((1)H-MRS) at a 7.0T MR and to define changes in apparent diffusion coefficient (ADC) values and the concentration of metabolites in the rat brain after acute EtOH intoxication. ADC values in each ROI decreased significantly at 1 h and 3 h after ethanol administration. ADC values in frontal lobe were decreased significantly compared with other regions at 3 h. For EtOH/Cr+PCr and cerebral metabolites (Cho, Tau, and Glu) differing over time, no significant differences for Ins, NAA, and Cr were observed in frontal lobes. Regression analysis revealed a significant association between TSEtOH/Cr+PCr and TSCho, TSTau, TSGlu, and TSADC. The changes of ADC values in different brain regions reflect the process of the cytotoxic edema in vivo. The characterization of frontal lobes metabolites changes and the correlations between TSEtOH/Cr+PCr and TSCho, TSTau, and TSGlu provide a better understanding for the biological mechanisms in neurotoxic effects of EtOH on the brain. In addition, the correlations between TSEtOH/Cr+PCr and TSADC will help us to understand development of the ethanol-induced brain cytotoxic edema.
Department of Radiology, The Second Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong 515041, China ; Department of Ultrasound, The Affiliated Yuebei People's Hospital of Shantou University Medical College, Shaoguan, Guangdong 512025, China.
Huimin
H
Liu
Department of Radiology, The Second Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong 515041, China.
Wenbin
0000-0002-1750-4999
W
Zheng
Department of Radiology, The Second Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong 515041, China.
Gen
G
Yan
Department of Neurosurgery, The Affiliated Yuebei People's Hospital of Shantou University Medical College, Shaoguan, Guangdong 512025, China.
Baoguo
B
Liu
Department of Radiology, The Second Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong 515041, China.
Lingmei
L
Kong
Department of Radiology, The Second Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong 515041, China.
Yan
Y
Ding
Department of Radiology, The Second Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong 515041, China.
Zhiwei
0000-0002-0153-363X
Z
Shen
Department of Radiology, The Second Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong 515041, China.
Hui
H
Tan
Department of Radiology, The Second Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong 515041, China.
Guishan
G
Zhang
D000435:Alcoholic Intoxication / Q000150:complications / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D001928:Brain Diseases, Metabolic / Q000139:chemically induced* / Q000209:etiology / Q000378:metabolism*; D004487:Edema / Q000139:chemically induced* / Q000378:metabolism*; D000431:Ethanol / Q000506:poisoning*; D008279:Magnetic Resonance Imaging; D008297:Male; D066244:Proton Magnetic Resonance Spectroscopy; D051381:Rats; D017207:Rats, Sprague-Dawley
D000431:Ethanol