Multiple protein stationary phases: a review.
Cellular membrane affinity chromatography stationary phases have been extensively used to characterize immobilized proteins and provide a direct measurement of multiple binding sites, including orthosteric and allosteric sites. This review will address the utilization of immobilized cellular and tissue fragments to characterize multiple transmembrane proteins co-immobilized onto a stationary phase. This approach will be illustrated by demonstrating that multiple transmembrane proteins were immobilized from cell lines and tissue fragments. In addition, the immobilization of individual compartments/organelles within a cell will be discussed and the changes in the proteins binding/kinetics based on their location.
Biomedical Research Center, National Institute on Aging, National Institutes of Health, 251 Bayview Boulevard, Suite 100, Baltimore, MD 21224, USA.
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NS
Singh
Department of Natural Sciences, Institute of Mathematics and Natural Sciences, Tallinn University, Narva mnt. 29, 10120 Tallinn, Estonia.
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KL
Habicht
Biomedical Research Center, National Institute on Aging, National Institutes of Health, 251 Bayview Boulevard, Suite 100, Baltimore, MD 21224, USA.
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KS
Dossou
Department of Natural Sciences, Institute of Mathematics and Natural Sciences, Tallinn University, Narva mnt. 29, 10120 Tallinn, Estonia.
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Shimmo
Biomedical Research Center, National Institute on Aging, National Institutes of Health, 251 Bayview Boulevard, Suite 100, Baltimore, MD 21224, USA.
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IW
Wainer
Biomedical Research Center, National Institute on Aging, National Institutes of Health, 251 Bayview Boulevard, Suite 100, Baltimore, MD 21224, USA. Electronic address: moaddelru@grc.nia.nih.gov.
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Moaddel
D001665:Binding Sites / Q000502:physiology; D002846:Chromatography, Affinity / Q000379:methods; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry* / Q000378:metabolism; D007700:Kinetics; D008024:Ligands; D008565:Membrane Proteins / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding / Q000502:physiology
D055767:Immobilized Proteins; D008024:Ligands; D008565:Membrane Proteins
ATP-binding cassette transporters; Bioaffinity chromatography; G-protein coupled receptors; Ligand-gated ion channels