Phylogenetic classification and clinical aspects of a new putative Deltapapillomavirus associated with skin lesions in cattle.
Bovine papillomaviruses (BPVs) are recognized as causal agents of benign and malignant tumors in cattle. Thirteen types of BPVs have already been described and classified into 3 distinct genera. Divergences in the nucleotide sequence of the L1 gene are used to identify new viral types through the employment of PCR assays with degenerated primers. In the present study, a method for identifying BPVs based on PCR-RFLP and DNA sequencing allowed the identification of a new putative Deltapapillomavirus, designated JN/3SP (JQ280500.1). The analysis of the L1 gene showed that this strain was most closely related to the BPVs -1, -2, -13 , and OaPV1 (71-73% genetic similarity). In this study, we describe the detection of this new putative Deltapapillomavirus type and verify its phylogenetic position within the genus.
Laboratório de Genética, Instituto Butantan, São Paulo, SP, Brasil.
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TC
Melo
Laboratório de Genética, Instituto Butantan, São Paulo, SP, Brasil.
R F
RF
Carvalho
Laboratório de Genética, Instituto Butantan, São Paulo, SP, Brasil.
J
J
Mazzucchelli-de-Souza
Laboratório de Genética, Instituto Butantan, São Paulo, SP, Brasil.
N
N
Diniz
Departamento de Botânica, Universidade Federal de Pernambuco, Recife, PE, Brasil.
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S
Vasconcelos
Laboratório de Genética, Instituto Butantan, São Paulo, SP, Brasil.
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SL
Assaf
Laboratório de Genética, Instituto Butantan, São Paulo, SP, Brasil.
R P
RP
Araldi
Laboratório de Genética, Instituto Butantan, São Paulo, SP, Brasil.
R M
RM
Ruiz
Laboratório de Genética, Instituto Butantan, São Paulo, SP, Brasil.
I
I
Kerkis
Laboratório de Genética, Instituto Butantan, São Paulo, SP, Brasil.
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W
Beçak
Laboratório de Genética, Instituto Butantan, São Paulo, SP, Brasil ritastocco@butantan.gov.br.
R C
RC
Stocco
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000235:genetics / Q000821:virology*; D052687:Deltapapillomavirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity; D010802:Phylogeny; D017422:Sequence Analysis, DNA