A DNA microarray-based assay to detect dual infection with two dengue virus serotypes.
Here; we have described and tested a microarray based-method for the screening of dengue virus (DENV) serotypes. This DNA microarray assay is specific and sensitive and can detect dual infections with two dengue virus serotypes and single-serotype infections. Other methodologies may underestimate samples containing more than one serotype. This technology can be used to discriminate between the four DENV serotypes. Single-stranded DNA targets were covalently attached to glass slides and hybridised with specific labelled probes. DENV isolates and dengue samples were used to evaluate microarray performance. Our results demonstrate that the probes hybridized specifically to DENV serotypes; with no detection of unspecific signals. This finding provides evidence that specific probes can effectively identify single and double infections in DENV samples.
Department of Genetics and Molecular Biology, Centro de Investigación y de Estudios Avanzados del IPN (CINVESTAV-IPN), Mexico D.F., 07360, Mexico. alvaro@cinvestav.mx.
Alvaro
A
Díaz-Badillo
Department of Genetics and Molecular Biology, Centro de Investigación y de Estudios Avanzados del IPN (CINVESTAV-IPN), Mexico D.F., 07360, Mexico. lmunoz@cinvestav.mx.
María de Lourdes
Mde L
Muñoz
Department of Genetics and Molecular Biology, Centro de Investigación y de Estudios Avanzados del IPN (CINVESTAV-IPN), Mexico D.F., 07360, Mexico. gperezr@cinvestav.mx.
Gerardo
G
Perez-Ramirez
Centro de Investigación en Micro y Nanotecnología (MICRONA), Universidad Veracruzana, Boca del Rio, Veracruz, 94294, Mexico. valtuzar@gmail.com.
Victor
V
Altuzar
Centro Internacional de Mejoramiento de Maíz y Trigo (CIMMYT), Texcoco, Estado de Mexico, 56237, Mexico. jabfpc@yahoo.com.
Juan
J
Burgueño
Department of Physics, Centro de Investigación y de Estudios Avanzados del IPN (CINVESTAV-IPN), Mexico D.F., 07360, Mexico. juliogma1@gmail.com.
Julio G
JG
Mendoza-Alvarez
Laboratorio Estatal de Salud Pública de Oaxaca, Servicios de Salud de Oaxaca, Oaxaca, 71257, Mexico. monterrey2bios@hotmail.com.
Jorge P
JP
Martínez-Muñoz
Escuela de Medicina Veterinaria y Zootecnia, Universidad Autónoma Benito Juarez de Oaxaca, 68120, Oaxaca, Mexico. alecis@msn.com.
Alejandro
A
Cisneros
División de Epidemiologia, Coordinación de Programas Integrados de Salud, Instituto Mexicano del Seguro Social, Mexico D.F., 06700, Mexico. joel.navarrete@imss.gob.mx.
Joel
J
Navarrete-Espinosa
Department of Physics, Centro de Investigación y de Estudios Avanzados del IPN (CINVESTAV-IPN), Mexico D.F., 07360, Mexico. fsanchez@fis.cinvestav.mx.
Feliciano
F
Sanchez-Sinencio
D015374:Biosensing Techniques / Q000295:instrumentation*; D004279:DNA, Viral / Q000235:genetics*; D003715:Dengue / Q000175:diagnosis* / Q000821:virology*; D003716:Dengue Virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D065288:Serogroup
D004279:DNA, Viral