Coexpression of ang1 and tie2 in odontoblasts of mouse developing and mature teeth-a new insight into dentinogenesis.
Angiopoietin-1 regulates vascular angiogenesis and stabilization, and is reported to promote bone formation by facilitating angiogenesis. To estimate the role of Ang1 in odontogenesis, we explored the distribution of Ang1 and the receptor, Tie2 in the mouse developing and mature first molar of the mandible. At embryonic day 18, when differentiation of odontoblasts begins, immunosignals for Ang1 were intensely detected in the basement membrane and the distal side, which faced the basement membrane of odontoblasts. In situ hybridization revealed that Ang1 was expressed in odontoblasts and ameloblasts facing the basement membrane. Tie2 was localized in the distal side of odontoblasts. After birth, Ang1 was detected in the predentin, whereas both Ang1 and Tie2 were colocalized in odontoblasts and odontoblast processes. These distributions were retained up to 8 weeks. In contrast to odontoblasts, ameloblasts, cementoblasts and osteoblasts expressed Ang1 but did not express Tie2. Colocalization of Ang1 and Tie2 in odontoblasts and selective expression of Tie2 in odontoblasts among cells responsible for calcified tissue formation suggested the involvement of autocrine signals of Ang1-Tie2 in dentinogenesis.
Department of Applied Prosthodontics, Nagasaki University Graduate School of Biomedical Sciences.
Kazunori
K
Nakajima
Department of Oral Pathology and Bone Metabolism, Nagasaki University Graduate School of Biomedical Sciences.
Yasuaki
Y
Shibata
Department of Histology and Cell Biology, Nagasaki University Graduate School of Biomedical Sciences ; Present address: Division of Histochemistry and Cell Biology, Department of Anatomy, Faculty of Medicine, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki 889-1692, Japan.
Yoshitaka
Y
Hishikawa
Central Electron Microscope Laboratory, Nagasaki University Graduate School of Biomedical Sciences, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan.
Takashi
T
Suematsu
Department of Cell Biology, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki 852-8588, Japan.
Masako
M
Mori
Department of Cell Biology, National Cerebral and Cardiovascular Center Research Institute, 5-7-1 Fujishirodai, Suita, Osaka 565-8565, Japan.
Shigetomo
S
Fukuhara
Department of Histology and Cell Biology, Nagasaki University Graduate School of Biomedical Sciences.
Takehiko
T
Koji
Department of Applied Prosthodontics, Nagasaki University Graduate School of Biomedical Sciences.
Takashi
T
Sawase
Department of Oral Pathology and Bone Metabolism, Nagasaki University Graduate School of Biomedical Sciences.
Tohru
T
Ikeda
Tie2; angiopoietin-1; dentinogenesis; odontoblast; tooth