[Effect of Yangjing Zhongyu Decoction on mRNA and protein expression of PCNA, StAR, and FSHR in ovarian granulosa cells cultured by excess androgen].
OBJECTIVE
To observe the effect of Yangjing Zhongyu Decoction (YZD) on mRNA and protein expression of PCNA, StAR, and FSHR in ovarian granulose cells (GCs) cultured by excess androgen.


METHODS
Ovarian GCs from porcine follicles were isolated and cultured in vitro. Follicular stimulating hormone (FSH) or YZD was added in the GCs treated by excess testosterone propionate. Totally 48 h later mRNA and protein expression of PCNA, StAR, and FSHR were detected by RT-PCR and Western blot.


RESULTS
Excess androgen inhibited mRNA and protein expression of PCNA, StAR, and FSHR of GCs. FSH and YZD could antagonize inhibition of excess androgens, and promote mRNA and protein expression of PCNA, StAR, and FSHR in GCs.


CONCLUSIONS
YZD could antagonize the inhibition of excess androgen on mRNA and protein expression of PCNA, StAR and FSHR in GCs. Thus, we inferred that YZD could improve the follicle dysplasia by promoting mRNA and protein expression of PCNA, StAR and FSHR in GCs.
Yan-Hua
YH
Zheng
Tao
T
Ding
Hong-Xia
HX
Ma
Dan-Feng
DF
Ye
Nian-Jun
NJ
Su
Xiao-Ke
XK
Wu
D000728:Androgens / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D006107:Granulosa Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D006080:Ovarian Follicle / Q000166:cytology / Q000187:drug effects; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D011962:Receptors, FSH / Q000235:genetics / Q000378:metabolism; D013552:Swine
D000728:Androgens; D004365:Drugs, Chinese Herbal; D026901:Membrane Transport Proteins; D018809:Proliferating Cell Nuclear Antigen; D012333:RNA, Messenger; D011962:Receptors, FSH; D005640:Follicle Stimulating Hormone