Expression of TSG101 protein and LSF transcription factor in HPV-positive cervical cancer cells.
Our previous study demonstrated a decreased expression of tumor susceptibility gene 101 (TSG101) in cervical cancer cells. To identify the mechanism responsible for TSG101 downregulation during cervical cancer development, we analyzed the TSG101 promoter using cis-element cluster finder software. One of the transcription factors whose binding site was detected in theTSG101promoter was late SV40 factor (LSF). The aim of this study was to analyze the TSG101 protein and LSF expression levels during cervical cancer development. Immunohistochemical analysis confirmed a previously observed decreased expression of TSG101, whereas quantitative polymerase chain reaction (qPCR) and immunohistochemistry analysis revealed high expression of LSF in cervical, precancer and cancer cells compared with human papillomavirus (HPV)-negative non-cancer samples. High expression of LSF in cervical cancer HPV-positive cells suggests that this protein may be important in the regulation of TSG101 expression, as well as in cervical carcinogenesis. The role of LSF as a mediator in cervical cancer development must be confirmed in future studies.
Department of Molecular Virology, Adam Mickiewicz University, Poznań 61-614, Poland.
Justyna K
JK
Broniarczyk
NanoBioMedical Centre, Adam Mickiewicz University, Poznań 61-614, Poland.
Alicja
A
Warowicka
Department of Obstetrics and Gynecology, Medical University of Lublin, Lublin 20-081, Poland.
Anna
A
Kwaśniewska
Department of Cell Biology, University of Medical Science, Poznan 60-806, Poland.
Maria
M
Wohuń-Cholewa
First Department of Oncological Gynecology and Gynecology, Medical University of Lublin, Lublin 20-081, Poland.
Wojciech
W
Kwaśniewski
Department of Molecular Virology, Adam Mickiewicz University, Poznań 61-614, Poland.
Anna
A
Goździcka-Józefiak
LSF transcription factor; TSG101 protein; cervical cancer