[Identification of a novel HLA allele, HLA-DRB1*03:80, by sequencing-based typing].
This study was aimed to identify a novel HLA-DRB1 allele from a Chinese potential hemopoietic stem cell donor of Northeast China. A rare HLA-DRB1 allele was initially detected by Luminex PCR-SSO typing, then the sample was sequenced by sequence-based typing (SBT) and the alignments of sample's alleles was identified by single allele-specific sequencing strategy. The results revealed the existence of a new allele which differs from the closest matching allele DRB1*03:06 by a single nucleotide substitution at position 239, where C→G in exon 2, resulting in an amino acid exchange from Thr to Arg at codon 51. It is concluded that a novel allele has been confirmed and its name DRB1*03:80 is officially assigned by the WHO Nomenclature Committee in February 2012.
HLA Typing Laboratory of China Marrow Donor Program, Blood Center of Shandong Province, Jinan 250014, Shandong Province, China.
Xiang-Min
XM
Nie
HLA Typing Laboratory of China Marrow Donor Program, Blood Center of Shandong Province, Jinan 250014, Shandong Province, China.
Yi
Y
Zhang
HLA Typing Laboratory of China Marrow Donor Program, Blood Center of Shandong Province, Jinan 250014, Shandong Province, China.
Yun-Long
YL
Zhuang
HLA Typing Laboratory of China Marrow Donor Program, Blood Center of Shandong Province, Jinan 250014, Shandong Province, China.
Yong-Hong
YH
Song
HLA Typing Laboratory of China Marrow Donor Program, Blood Center of Shandong Province, Jinan 250014, Shandong Province, China.
Wen-Ben
WB
Qiao
HLA Typing Laboratory of China Marrow Donor Program, Blood Center of Shandong Province, Jinan 250014, Shandong Province, China.
Yan
Y
Liu
HLA Typing Laboratory of China Marrow Donor Program, Blood Center of Shandong Province, Jinan 250014, Shandong Province, China. E-mail:zhuchuanfu2008@tom.com.
Chuan-Fu
CF
Zhu
D000483:Alleles; D044466:Asian People / Q000235:genetics; D059811:HLA-DRB1 Chains / Q000235:genetics*; D006801:Humans; D008297:Male; D017422:Sequence Analysis, DNA
D059811:HLA-DRB1 Chains