Effect on eNOS/NO Pathway in MIRI rats with preconditioning of GFPC from Dang Gui Si Ni decoction.
OBJECTIVE
In order to discover whether the eNOS/NO (endothelial nitric oxide synthase/nitric oxide) pathway is involved in the protective mechanisms of ischemic myocardium of DGSND (Dang Gui Si Ni Decoction) in MIRI (myocardial ischemia-reperfusion injury) SD rats.


METHODS
We made I/R (ischemia-reperfusion) model by ligating the left anterior-descending branch of the coronary artery (LAD) for 30 min and releasing the ligature for 120 min. eNOS (nitric oxide synthase) mRNA (message ribonucleic acid) and iNOS (inducible nitric oxide synthase) mRNA were measured by the methods of real-time RT-PCR (Real time Polychainase Chain Reaction), peNOS (phosphorylated eNOS) and iNOS protein were measured by the means of western blot.


RESULTS
In PPC group, real-time RT-PCR and western-blot analysis showed that eNOS mRNA and peNOS protein increased markedly (P < 0.05); iNOS mRNA and protein decreased significantly (P < 0.05).


CONCLUSIONS
These results indicate that ischemic preconditioning (IPC) of GFPC from DGSND plays a protective role in I/R heart through regulating the eNOS/NO signal pathway, which could increase the eNOS gene expression and decrease the expression of iNOS mRNA.
Nursing Science Department of Huanghuai University, Zhumadian, China.
Guo-Qiang
GQ
Qian
Tradition Chinese Medicine Department of Medical College, Jinan University, Guangzhou, China.
Xia
X
Peng
Tradition Chinese Medicine Department of Medical College, Jinan University, Guangzhou, China.
Chuan
C
Cai
Tradition Chinese Medicine Department of Medical College, Jinan University, Guangzhou, China.
Guo-Ping
GP
Zhao
Cinnamic acid; MIRI; NO; eNOS; ferulic acid; glycyrrhizic acid; paeoniflorin