Isolation and characterization of a novel hydrocarbon-degrading bacterium Achromobacter sp. HZ01 from the crude oil-contaminated seawater at the Daya Bay, southern China.
Microorganisms play an important role in the biodegradation of petroleum contaminants, which have attracted great concern due to their persistent toxicity and difficult biodegradation. In this paper, a novel hydrocarbon-degrading bacterium HZ01 was isolated from the crude oil-contaminated seawater at the Daya Bay, South China Sea, and identified as Achromobacter sp. Under the conditions of pH 7.0, NaCl 3% (w/v), temperature 28 °C and rotary speed 150 rpm, its degradability of the total n-alkanes reached up to 96.6% after 10 days of incubation for the evaporated diesel oil. Furthermore, Achromobacter sp. HZ01 could effectively utilize polycyclic aromatic hydrocarbons (PAHs) as its sole carbon source, and could remove anthracene, phenanthrene and pyrence about 29.8%, 50.6% and 38.4% respectively after 30 days of incubation. Therefore, Achromobacter sp. HZ01 may employed as an excellent degrader to develop one cost-effective and eco-friendly method for the bioremediation of marine environments polluted by crude oil.
Guangdong Provincial Education Department Key Laboratory of Marine Petroleum Exploration and Development, School of Marine Sciences, Sun Yat-Sen University, Guangzhou 510006, People's Republic of China; Department of Food and Bioengineering, Guangdong Industry Technical College, Guangzhou 510300, People's Republic of China.
Mao-Cheng
MC
Deng
College of Environment and Energy, South China University of Technology, Guangzhou 510641, People's Republic of China; Department of Food and Bioengineering, Guangdong Industry Technical College, Guangzhou 510300, People's Republic of China.
Jing
J
Li
Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, School of Marine Sciences, Sun Yat-Sen University, Guangzhou 510006, People's Republic of China.
Fu-Rui
FR
Liang
Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, School of Marine Sciences, Sun Yat-Sen University, Guangzhou 510006, People's Republic of China.
Meisheng
M
Yi
Guangdong Provincial Education Department Key Laboratory of Marine Petroleum Exploration and Development, School of Marine Sciences, Sun Yat-Sen University, Guangzhou 510006, People's Republic of China.
Xiao-Ming
XM
Xu
Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, School of Marine Sciences, Sun Yat-Sen University, Guangzhou 510006, People's Republic of China.
Jian-Ping
JP
Yuan
Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, School of Marine Sciences, Sun Yat-Sen University, Guangzhou 510006, People's Republic of China.
Juan
J
Peng
Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, School of Marine Sciences, Sun Yat-Sen University, Guangzhou 510006, People's Republic of China. Electronic address: hyh-409@163.com.
Chou-Fei
CF
Wu
Guangdong Provincial Education Department Key Laboratory of Marine Petroleum Exploration and Development, School of Marine Sciences, Sun Yat-Sen University, Guangzhou 510006, People's Republic of China. Electronic address: wangjhai@mail.sysu.edu.cn.
Jiang-Hai
JH
Wang
D042402:Achromobacter / Q000302:isolation & purification* / Q000378:metabolism; D000473:Alkanes / Q000378:metabolism; D061006:Bays / Q000382:microbiology; D001673:Biodegradation, Environmental; D002681:China; D010578:Petroleum / Q000032:analysis / Q000378:metabolism* / Q000382:microbiology; D059392:Petroleum Pollution / Q000517:prevention & control*; D010616:Phenanthrenes / Q000378:metabolism; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism*; D012623:Seawater / Q000382:microbiology*
D000473:Alkanes; D010578:Petroleum; D010616:Phenanthrenes; D011084:Polycyclic Aromatic Hydrocarbons; C031181:phenanthrene
Achromobacter sp.; Biodegradation; Diesel oil; Petroleum hydrocarbon