Complete genome sequence of a novel phage, vB_MoxS-ISF9, infecting methylotrophic Microbacterium: first report of a virulent Microbacterium phage.
Here, we report the first genome sequence of a new virulent phage of Microbacterium oxydans, termed vB_MoxS-ISF9, which was isolated from sewage. Transmission electron microscopy showed that the isolated phage, which has a hexagonal head of about 80 nm in diameter and a long non-contractile tail of about 240 nm, belongs to the family Siphoviridae. The vB_MoxS-ISF9 DNA was completely sequenced and found to be 59,254 bp in length, with a G+C content of 62.76% and 120 putative open reading frames (ORFs). The predicted protein products of the ORFs were identified, and their sequences were analyzed. In a comparison with all available phage genomes, vB_MoxS-ISF9 did not show any significant similarity to other previously reported bacteriophages. To the beast of our knowledge, this is the first report of the isolation and complete genomic sequencing of a virulent phage against a member of the genus Microbacterium.
Department of Biology, Faculty of Science, University of Isfahan, Hezar Jereeb Street, 81746-73441, Isfahan, Iran.
Isaac
I
Zamani
Majid
M
Bouzari
Giti
G
Emtiazi
Seyed Mahdi
SM
Ghasemi
Hyo-Ihl
HI
Chang
D000192:Actinomycetales / Q000821:virology*; D001435:Bacteriophages / Q000235:genetics* / Q000302:isolation & purification* / Q000648:ultrastructure; D001482:Base Composition; D004279:DNA, Viral / Q000737:chemistry* / Q000235:genetics*; D023061:Gene Order; D016679:Genome, Viral; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000821:virology; D017906:Siphoviridae / Q000235:genetics / Q000302:isolation & purification / Q000648:ultrastructure; D014771:Virion / Q000648:ultrastructure
D004279:DNA, Viral; D012722:Sewage