The application of transcription activator-like effector nucleases for genome editing in C. elegans.
The nematode Caenorhabditis elegans has been a powerful model system for biomedical research in the past decades, however, the efficient genetic tools are still demanding for gene knockout, knock-in or conditional gene mutations. Transcription activator-like effector nucleases (TALENs) that comprise a sequence-specific DNA-binding domain fused to a FokI nuclease domain facilitate the targeted genome editing in various cell types or organisms. Here we summarize the recent progresses and protocols using TALENs in C. elegans that generate gene mutations and knock-ins in the germ line and the conditional gene knockout in somatic tissues.
National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Peishan
P
Yi
School of Medicine, Tsinghua University, Beijing 100084, China. Electronic address: weili.oulab@gmail.com.
Wei
W
Li
Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China. Electronic address: guangshuo.ou@gmail.com.
Guangshuo
G
Ou
D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics*; D003851:Deoxyribonucleases / Q000235:genetics*; D055786:Gene Knockout Techniques; D018390:Gene Targeting / Q000379:methods*; D016678:Genome; D015533:Transcriptional Activation / Q000235:genetics*
D003851:Deoxyribonucleases
Caenorhabditis elegans; Conditional gene knockout; Mutation; Transcription activator-like effector nucleases (TALENs)