Phytochemical analysis and antioxidant capacity of Tabernaemontana catharinensis A. DC. Fruits and branches.
The antioxidant capacity of the crude extract and fractions of Tabernaemontana catharinensis fruits and branches, was evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method and the content of polyphenols, flavonoids, alkaloids and condensed tannins were determined by the spectrophotometric method. The ethyl acetate fraction of the fruits and the n-butanol fraction of the branches showed IC50 of 181.82 µg/mL and 78.19 µg/mL, respectively. All fractions were analyzed by high performance liquid chromatography (HPLC), in the branches were quantified chlorogenic acid in the chloroform (8.96 mg/g), ethyl acetate (4.31 mg/g) and n-butanol (3.33 mg/g) fractions; caffeic acid in the ethyl acetate (5.24 mg/g) and n-butanol (1.81 mg/g); gallic acid (0.52 mg/g) in the n-butanol. In the fruits, chlorogenic acid in the chloroform (1.67 mg/g); rutin in the ethyl acetate (3.45 mg/g) and n-butanol (8.98 mg/g) fractions. The present study showed that these quantified compounds can contribute to antioxidant capacity which was higher in the branches than in the fruits.
Laboratório de Fitoquímica, Departmento de Farmácia Industrial, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil.
Mariana
M
Piana
Laboratório de Fitoquímica, Departmento de Farmácia Industrial, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil.
Aline A
AA
Boligon
Laboratório de Fitoquímica, Departmento de Farmácia Industrial, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil.
Thiele F DE
TF
Brum
Laboratório de Fitoquímica, Departmento de Farmácia Industrial, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil.
Marina
M
Zadra
Laboratório de Fitoquímica, Departmento de Farmácia Industrial, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil.
Bianca V
BV
Belke
Laboratório de Fitoquímica, Departmento de Farmácia Industrial, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil.
Amanda L F
ALF
Froeder
Laboratório de Fitoquímica, Departmento de Farmácia Industrial, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil.
Janaína K
JK
Frohlich
Laboratório de Fitoquímica, Departmento de Farmácia Industrial, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil.
Letícia T
LT
Nunes
Laboratório de Fitoquímica, Departmento de Farmácia Industrial, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil.
Lauren
L
Pappis
Departamento de Fitotecnia, Campus São Gabriel, Universidade Federal do Pampa, São Gabriel, RS, Brasil.
Alexandra A
AA
Boligon
Laboratório de Fitoquímica, Departmento de Farmácia Industrial, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil.
Margareth L
ML
Athayde