[An efficient way in mouse brain dissection].
Laboratory mice are common experimental animals in biological, medical, pharmacological and psychological researches primarily because they are easy to maintain and reproduce quickly. The protection of the welfare of experimental animals is gaining greater attention during the application of a large number of mice. It's therefore essential to consider how to reduce the unnecessary use of animals and fully exploit each experimental animal. We report, in this article, an efficient way to dissect various brain regions from a mouse for protein immunoblot and/or neuronal culture, providing technical reference information for minimizing the number of animals used in projects, and refining methods and procedures to quick brain dissection.
School of Psychology; School of Life Sciences, South China Normal University, Guangzhou 510631, China. yangli08@scnu.edu.cn.
Jin-Zhao
JZ
Wang
Cheng
C
Long
Li
L
Yang
D000827:Animal Welfare; D000818:Animals; D001921:Brain / Q000033:anatomy & histology*; D004210:Dissection / Q000379:methods*; D051379:Mice