Transcriptome profile of human neuroblastoma cells in the hypomagnetic field.
Research has shown that the hypomagnetic field (HMF) can affect embryo development, cell proliferation, learning and memory, and in vitro tubulin assembly. In the present study, we aimed to elucidate the molecular mechanism by which the HMF exerts its effect, by comparing the transcriptome profiles of human neuroblastoma cells exposed to either the HMF or the geomagnetic field. A total of 2464 differentially expressed genes (DEGs) were identified, 216 of which were up-regulated and 2248 of which were down-regulated after exposure to the HMF. These DEGs were found to be significantly clustered into several key processes, namely macromolecule localization, protein transport, RNA processing, and brain function. Seventeen DEGs were verified by real-time quantitative PCR, and the expression levels of nine of these DEGs were measured every 6 h. Most notably, MAPK1 and CRY2, showed significant up- and down-regulation, respectively, during the first 6 h of HMF exposure, which suggests involvement of the MAPK pathway and cryptochrome in the early bio-HMF response. Our results provide insights into the molecular mechanisms underlying the observed biological effects of the HMF.
Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, China.
WeiChuan
W
Mo
Ying
Y
Liu
Perry F
PF
Bartlett
RongQiao
R
He
D001483:Base Sequence; D001921:Brain / Q000502:physiology; D045744:Cell Line, Tumor; D002843:Chromatin / Q000378:metabolism; D003599:Cytoskeleton / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D015972:Gene Expression Regulation, Neoplastic; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D020935:MAP Kinase Signaling System; D060526:Magnetic Fields; D008969:Molecular Sequence Data; D009447:Neuroblastoma / Q000378:metabolism*; D012313:RNA / Q000737:chemistry / Q000378:metabolism; D015398:Signal Transduction; D013997:Time Factors; D059467:Transcriptome; D015854:Up-Regulation
D002843:Chromatin; D012313:RNA