Anethole and eugenol reduce in vitro and in vivo leukocyte migration induced by fMLP, LTB4, and carrageenan.
The aim of this study was to investigate the effect of anethole (AN) and eugenol (EUG) on leukocyte migration using in vitro chemotaxis and in situ microcirculation assays. BALB/c mice were used for the in vitro chemotaxis assay, and Wistar rats for the in situ microcirculation assay. We evaluated (a) the in vitro leukocyte migration in response to chemotactic factors (formyl-methionyl-leucyl-phenylalanine [fMLP] and leukotriene B4 [LTB4]) and (b) the rolling, adhesion, and migration of leukocytes induced by an injection of carrageenan (100 µg/cavity) into the scrotum of the animal. In the in vitro chemotaxis assay, AN and EUG at doses of 1, 3, 9, and 27 µg/ml significantly inhibited leukocyte migration when stimulated by the chemotactic agents fMLP and LTB4. In the in situ microcirculation assay, AN at doses of 125 and 250 mg/kg and EUG at a dose of 250 mg/kg significantly decreased the number of leukocytes that rolled, adhered, and migrated to perivascular tissue. The results indicate that AN and EUG exert inhibitory effects on leukocyte migration, highlighting their possible use to diminish excessive leukocyte migration in the inflammatory process.
Laboratory of Inflammation, Department of Pharmacology and Therapeutic, State University of Maringá, Maringá, PR, Brazil.
Camila Fernanda
CF
Estevão-Silva
Raquel
R
Kummer
Fernanda Carolina
FC
Fachini-Queiroz
Renata
R
Grespan
Gessilda Alcântara
GA
Nogueira de Melo
Silmara
S
Baroni
Roberto Kenji Nakamura
RK
Cuman
Ciomar Aparecida
CA
Bersani-Amado
D000081248:Allylbenzene Derivatives; D000818:Animals; D000840:Anisoles / Q000494:pharmacology*; D002351:Carrageenan / Q000494:pharmacology; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D005054:Eugenol / Q000494:pharmacology*; D006801:Humans; D007962:Leukocytes / Q000187:drug effects / Q000502:physiology; D007975:Leukotriene B4 / Q000494:pharmacology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
D000081248:Allylbenzene Derivatives; D000840:Anisoles; D007975:Leukotriene B4; D005054:Eugenol; D009240:N-Formylmethionine Leucyl-Phenylalanine; D002351:Carrageenan; C006578:anethole