An assessment of the diversity of culturable bacteria from main root of sugar beet.
The partial sequences of the 16S rRNA genes of 531 bacteria isolated from the main root of the sugar beet (Beta vulgaris L.) were determined and subsequently grouped into 155 operational taxonomic units by clustering analysis (≥99% identity). The most abundant phylum was Proteobacteria (72.5-77.2%), followed by Actinobacteria (9.8-16.6%) and Bacteroidetes (4.3-15.4%). Alphaproteobacteria (46.7-64.8%) was the most dominant class within Proteobacteria. Four strains belonging to Verrucomicrobia were also isolated. Phylogenetic analysis revealed that the Verrucomicrobia bacterial strains were closely related to Haloferula or Verrucomicrobium.
Hokkaido Agricultural Research Center, National Agriculture and Food Research Organization.
Kazuyuki
K
Okazaki
Takao
T
Iino
Yosuke
Y
Kuroda
Kazunori
K
Taguchi
Hiroyuki
H
Takahashi
Takuji
T
Ohwada
Hiroto
H
Tsurumaru
Takashi
T
Okubo
Kiwamu
K
Minamisawa
Seishi
S
Ikeda
D039903:Actinobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D020561:Alphaproteobacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D041963:Bacteroidetes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D027461:Beta vulgaris / Q000382:microbiology*; D044822:Biodiversity; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology; D020560:Proteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S