Stimulation with the Aureobasidium pullulans-produced β-glucan effectively induces interferon stimulated genes in macrophage-like cell lines.
A β-(1,3),(1,6)-D-glucan produced by A. pullulans (AP-PG) is known to be an immune stimulating agent. In this study, we demonstrate that the stimulation with AP-PG effectively induces the interferon (IFN) stimulated genes (ISGs) in macrophage-like cell lines. The ISGs, Mx1, ISG15, and viperin mRNAs were significantly increased in RAW264.7 cells after stimulation with AP-PG. The stimulation with AP-PG transiently induced IFN-β mRNA. However, the expression of viperin mRNA was also increased after stimulation with AP-PG even when new protein synthesis was completely blocked by treatment with cycloheximide. Further, in IFN-α receptor knockdown RAW264.7 cells, AP-PG stimulation more effectively induced viperin mRNA compared with that of IFN-α stimulation. The phosphorylation of Ser 727 in STAT1 involved in the enhancement of STAT1 activation was immediately increased after stimulation with AP-PG. In addition, viperin mRNA expression induced after stimulation with IFN-α was significantly increased by combined stimulation with AP-PG. These results suggest that stimulation with AP-PG effectively induces the ISGs through the induction of IFN and the enhancement of STAT1-mediated transcriptional activation.
Aureo Science Co., Ltd., Hokudai Business Spring, North 21, West 12, Kita-ku, Sapporo, Hokkaido, 001-0021 Japan.
Daisuke
D
Muramatsu
1] Aureo Science Co., Ltd., Hokudai Business Spring, North 21, West 12, Kita-ku, Sapporo, Hokkaido, 001-0021 Japan [2] Aureo Co., Ltd., 54-1 Kazusakoito, Kimitsu, Chiba, 292-1149 Japan.
Koji
K
Kawata
Aureo Science Co., Ltd., Hokudai Business Spring, North 21, West 12, Kita-ku, Sapporo, Hokkaido, 001-0021 Japan.
Shiho
S
Aoki
Aureo Co., Ltd., 54-1 Kazusakoito, Kimitsu, Chiba, 292-1149 Japan.
Hirofumi
H
Uchiyama
Aureo Co., Ltd., 54-1 Kazusakoito, Kimitsu, Chiba, 292-1149 Japan.
Mitsuyasu
M
Okabe
Department of Probiotics Immunology, Institute for Genetic Medicine, Hokkaido University, North 15, West 7, Kita-ku, Sapporo, Hokkaido, 060-0815 Japan.
Tadaaki
T
Miyazaki
1] Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, North 18, West 9, Kita-ku, Sapporo, Hokkaido, 060-0818, Japan [2] Hokkaido University Research Center for Zoonosis Control, North 20, West 10, Kita-ku, Sapporo, Hokkaido, 001-0020, Japan [3] OIE Reference Laboratory for Highly Pathogenic Avian Influenza, Sapporo, Hokkaido, Japan.
Hiroshi
H
Kida
1] Aureo Science Co., Ltd., Hokudai Business Spring, North 21, West 12, Kita-ku, Sapporo, Hokkaido, 001-0021 Japan [2] Aureo Co., Ltd., 54-1 Kazusakoito, Kimitsu, Chiba, 292-1149 Japan.
Atsushi
A
Iwai
D000818:Animals; D001203:Ascomycota / Q000378:metabolism*; D002460:Cell Line; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D009980:Influenza A virus / Q000187:drug effects; D007370:Interferon Type I / Q000096:biosynthesis; D007372:Interferons / Q000378:metabolism*; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism*; D051379:Mice; D017471:Receptors, Interferon / Q000378:metabolism; D050794:STAT1 Transcription Factor / Q000378:metabolism; D015398:Signal Transduction; D047071:beta-Glucans / Q000494:pharmacology*
D007370:Interferon Type I; D017471:Receptors, Interferon; D050794:STAT1 Transcription Factor; D047071:beta-Glucans; D007372:Interferons