Photopolymerizable chitosan-collagen hydrogels for bone tissue engineering.
Photopolymerizable hydrogels derived from naturally occurring polymers have attracted significant interest in tissue-engineering applications due to their excellent biocompatibility, hydrophilic nature favourable for cell ingrowth and ability to be cured in situ through a minimally invasive procedure. In this study, we developed a composite hydrogel consisting of photocrosslinkable methacrylated glycol chitosan (MeGC) and semi-interpenetrating collagen (Col) with a riboflavin photoinitiator under blue light. The incorporation of Col in MeGC hydrogels enhanced the compressive modulus and slowed the degradation rate of the hydrogels. MeGC-Col composite hydrogels significantly enhanced cellular attachment, spreading, proliferation and osteogenic differentiation of mouse bone marrow stromal cells (BMSCs) seeded on the hydrogels compared with pure MeGC hydrogels, as observed by upregulated alkaline phosphatase (ALP) activity as well as increased mineralization. Similarly, when cells were encapsulated within hydrogels, BMSCs exhibited greater proliferation, ALP activity and mineral deposits in the presence of Col. These findings demonstrate that MeGC-Col composite hydrogels may be useful in promoting bone regeneration. Copyright © 2014 John Wiley & Sons, Ltd.
Department of Bioengineering, University of California, Los Angeles, CA, USA.
Christopher
C
Arakawa
Department of Bioengineering, University of California, Los Angeles, CA, USA.
Ronald
R
Ng
Department of Bioengineering, University of California, Los Angeles, CA, USA.
Steven
S
Tan
Department of Bioengineering, University of California, Los Angeles, CA, USA.
Soyon
S
Kim
Department of Bioengineering, University of California, Los Angeles, CA, USA.|Division of Advanced Prosthodontics, University of California, Los Angeles, CA, USA.
Benjamin
B
Wu
Department of Bioengineering, University of California, Los Angeles, CA, USA.|Division of Advanced Prosthodontics, University of California, Los Angeles, CA, USA.
Min
M
Lee
D000469:Alkaline Phosphatase / Q000737:chemistry; D000818:Animals; D001842:Bone and Bones / Q000737:chemistry*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry*; D003094:Collagen / Q000737:chemistry*; D020100:Hydrogels / Q000737:chemistry*; D008027:Light; D059630:Mesenchymal Stem Cells / Q000166:cytology; D051379:Mice; D010012:Osteogenesis; D010777:Photochemistry; D011108:Polymers / Q000737:chemistry; D023822:Tissue Engineering / Q000379:methods*
D020100:Hydrogels; D011108:Polymers; C118638:glycol-chitosan; D003094:Collagen; D048271:Chitosan; D000469:Alkaline Phosphatase
bone; chitosan; collagen; hydrogel; photopolymerization; tissue engineering