Isolation and characterization of isopiperitenol dehydrogenase from piperitenone-type Perilla.
Studying the biosynthesis of oil compounds in Perilla will help to elucidate regulatory systems for secondary metabolites and reaction mechanisms for natural product synthesis. In this study, two types of alcohol dehydrogenases, isopiperitenol dehydrogenases 1 and 2 (ISPD1 and ISPD2), which are thought to participate the oxidation of isopiperitenol in the biosynthesis of perilla, were isolated from three pure lines of perilla. Both ISPD1 and ISPD2 oxidized isopiperitenol into isopiperitenone with an oxidized form of nicotinamide adenine dinucleotide (NAD(+)) cofactor. ISPD1 used both isopiperitenol diastereomers, whereas ISPD2 used cis-isomer as a substrate. However, only ISPD2 was isolated from piperitenone-type perilla. These results suggests that in perilla, ISPD2 is related to the biosynthesis of piperitenone, which was formed via (-)-cis-isopiperitenol.
Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyoto University.
Naoko
N
Sato-Masumoto
Michiho
M
Ito
D000429:Alcohol Oxidoreductases / Q000302:isolation & purification* / Q000378:metabolism*; D000595:Amino Acid Sequence; D053898:Biosynthetic Pathways; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D039821:Monoterpenes / Q000378:metabolism*; D009243:NAD / Q000378:metabolism; D064430:NAD (+) and NADP (+) Dependent Alcohol Oxidoreductases; D009822:Oils, Volatile; D031343:Perilla / Q000201:enzymology*; D013729:Terpenes / Q000378:metabolism
D039821:Monoterpenes; D009822:Oils, Volatile; D013729:Terpenes; D009243:NAD; C044945:isopiperitenol; C044944:isopiperitenone; D000429:Alcohol Oxidoreductases; D064430:NAD (+) and NADP (+) Dependent Alcohol Oxidoreductases; C044952:isopiperitenol dehydrogenase; C044956:piperitenone