[Protein expressions of HDAC1 and DNMT1 in non-small-cell lung cancer and its clinical significance].
OBJECTIVE
To detect the serum protein expressions of histone deacetylase 1 (HDAC1) and DNA methyltransferase 1 (DNMT1) in patients with non-small-cell lung cancer and explore the correlation between their expressions and clinicopathological features.


METHODS
Enzyme-linked immunosorbent assay (ELISA) was used to measure the protein expressions of HDAC1 and DNMT1 in 136 lung cancer patients hospitalized at Department of Respiratory Diseases, First Affiliated Hospital,Zhengzhou University from September 2012 to June 2013 (cancer group). And 147 healthy physical examination subjects from Sixth People's Hospital of Zhengzhou were selected as controls and their clinicopathological features analyzed.


RESULTS
The protein expressions of HDAC1 and DNMT1 in patients with lung cancer (145 ± 53, 50 ± 11) were higher than those in control group (78 ± 56, 27 ± 6). And there was statistical significance (t = 596.16, 152.64, both P = 0.000) . The protein expressions of HDAC1 and DNMT1 were correlated with gender, smoking history and age (among P < 0.05). There was a positive association between the protein expressions of HDAC1 and DNMT1 in non-small-cell lung cancer (r = 0.525, P = 0.000). Statistical significance existed between the protein expressions of HDAC1 and DNMT1 and histological types or different stages (all P < 0.01).


CONCLUSIONS
The higher protein expressions of HDAC1 and DNMT1 may play an important role in the early process of lung cancer.
Department of Respiratory Diseases, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, China.
Yuejing
Y
Feng
Email: wangjing@zzu.edu.cn.
Jing
J
Wang
Yongjun
Y
Wu
Xu
X
Feng
Yongmin
Y
Yu
D000368:Aged; D002289:Carcinoma, Non-Small-Cell Lung / Q000097:blood* / Q000473:pathology; D016022:Case-Control Studies; D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000097:blood* / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D056284:Histone Deacetylase 1 / Q000097:blood* / Q000235:genetics; D006801:Humans; D008175:Lung Neoplasms / Q000097:blood* / Q000473:pathology; D008297:Male; D008875:Middle Aged
D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases; C000619380:DNMT1 protein, human; C110550:HDAC1 protein, human; D056284:Histone Deacetylase 1