Altered expression of cytokines in mice infected intranasally with two syncytial variants of Herpes simplex virus type 1.
Immune evasion strategies are important for the onset and the maintenance of viral infections. Many viruses have evolved mechanisms to counteract or suppress the host immune response. We have previously characterized two syncytial (syn) variants of Herpes simplex 1 (HSV-1) strain F, syn14-1 and syn17-2, obtained by selective pressure with a natural carrageenan. These variants showed a differential pathology in vaginal and respiratory mucosa infection in comparison with parental strain. In this paper, we evaluated the modulation of immune response in respiratory mucosa by these HSV-1 variants. We observed altered levels of Tumor Necrosis Factor-α and Interleukin-6 in lungs of animals infected with the syn14-1 and syn17-2 variants compared with the parental strain. Also, we detected differences in the recruitment of immune cells to the lung in syn variants infected mice. Both variants exhibit one point mutation in the sequence of the gene of glycoprotein D detected in the ectodomain of syn14-1 and the cytoplasmic tail of syn17-2. Results obtained in the present study contribute to the characterization of HSV-1 syn variants and the participation of the cellular inflammatory response in viral pathogenesis.
Laboratorio de Virología - Departamento de Química Biológica, Pab.2, Piso 4. Ciudad Universitaria, 1428EGA Buenos Aires, Argentina; IQUIBICEN (CONICET-UBA), Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria, Pab.2, Piso 4. Ciudad Universitaria, 1428EGA Buenos Aires, Argentina.
M
M
Carolina Artuso
Laboratorio de Virología - Departamento de Química Biológica, Pab.2, Piso 4. Ciudad Universitaria, 1428EGA Buenos Aires, Argentina.
Florencia N
FN
Linero
Laboratorio de Biología Tumoral - Departamento de Química Biológica, Pab.2, Piso 4. Ciudad Universitaria, 1428EGA Buenos Aires, Argentina; IQUIBICEN (CONICET-UBA), Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria, Pab.2, Piso 4. Ciudad Universitaria, 1428EGA Buenos Aires, Argentina.
Silvina
S
Gazzaniga
Laboratorio de Virología - Departamento de Química Biológica, Pab.2, Piso 4. Ciudad Universitaria, 1428EGA Buenos Aires, Argentina.
Luis A
LA
Scolaro
Laboratorio de Virología - Departamento de Química Biológica, Pab.2, Piso 4. Ciudad Universitaria, 1428EGA Buenos Aires, Argentina; IQUIBICEN (CONICET-UBA), Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria, Pab.2, Piso 4. Ciudad Universitaria, 1428EGA Buenos Aires, Argentina.
Carlos A
CA
Pujol
Laboratorio de Biología Tumoral - Departamento de Química Biológica, Pab.2, Piso 4. Ciudad Universitaria, 1428EGA Buenos Aires, Argentina; IQUIBICEN (CONICET-UBA), Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria, Pab.2, Piso 4. Ciudad Universitaria, 1428EGA Buenos Aires, Argentina.
Rosa
R
Wainstok
Laboratorio de Virología - Departamento de Química Biológica, Pab.2, Piso 4. Ciudad Universitaria, 1428EGA Buenos Aires, Argentina; IQUIBICEN (CONICET-UBA), Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria, Pab.2, Piso 4. Ciudad Universitaria, 1428EGA Buenos Aires, Argentina. Electronic address: majoc@qb.fcen.uba.ar.
M
M
Josefina Carlucci
D000818:Animals; D016207:Cytokines / Q000378:metabolism*; D005260:Female; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009092:Mucous Membrane / Q000276:immunology / Q000473:pathology; D017354:Point Mutation; D012141:Respiratory Tract Infections / Q000276:immunology* / Q000473:pathology* / Q000821:virology; D014759:Viral Envelope Proteins / Q000235:genetics
D016207:Cytokines; D014759:Viral Envelope Proteins; C039447:glycoprotein D, Human herpesvirus 1
Cytokines; Herpes; Mouse; Pathology; Virulence