Gene expression regulation of Bcl2, Bax and cytochrome-C by geraniol on chronic MPTP/probenecid induced C57BL/6 mice model of Parkinson's disease.
Parkinson's disease (PD) is a common disabling movement disorder owing to progressive depletion of dopamine in nigrostriatal region, and can be experimentally accelerated by the neurotoxin 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP). MPTP-treated mice are a representative animal model for searching for the therapeutic agents for PD without adverse effect. In this study we investigated the effect of geraniol (GE) on chronic MPTP/probenecid (MPTP/p) induced apoptotic changes in nigrostriatal region. We observed that chronic exposure to MPTP/p led to increased expression of apoptotic markers, results in neurodegeneration and motor behavioral impairments in mice. Pretreatment with GE to MPTP/p significantly improved motor functions and ameliorated striatal antioxidant balance. In addition, GE attenuated the expression of apoptotic markers evident by the normalized Bcl-2/Bax ratio and decreased expression of cytochrome-C and caspase-9 in the substantia nigra and striatum of MPTP/p induced mice model of PD. The findings of the present study suggested that GE, a new therapeutic potential avenue may have beneficial effects in slowing or preventing the progression of PD and other neurodegenerative disorders.
Division of Biochemistry, Faculty of Medicine, Raja Muthaiah Medical College, Annamalai University, Annamalainagar, Tamilnadu, India. Electronic address: k_r_rekha@yahoo.co.in.
Karamkolly R
KR
Rekha
Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar, Tamilnadu, India.
Govindasamy P
GP
Selvakumar
D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine / Q000008:administration & dosage; D000080462:Acyclic Monoterpenes; D000818:Animals; D017209:Apoptosis / Q000502:physiology; D015153:Blotting, Western; D002374:Catalase / Q000097:blood; D045304:Cytochromes c / Q000235:genetics* / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000097:blood; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009043:Motor Activity / Q000187:drug effects; D010300:Parkinson Disease / Q000097:blood / Q000188:drug therapy* / Q000235:genetics* / Q000378:metabolism; D011339:Probenecid / Q000008:administration & dosage; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics; D011897:Random Allocation; D060888:Real-Time Polymerase Chain Reaction; D013482:Superoxide Dismutase / Q000097:blood; D013729:Terpenes / Q000494:pharmacology*; D051028:bcl-2-Associated X Protein / Q000235:genetics* / Q000378:metabolism
D000080462:Acyclic Monoterpenes; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D013729:Terpenes; D051028:bcl-2-Associated X Protein; D045304:Cytochromes c; D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; C007836:geraniol; D011339:Probenecid
Apoptosis; Geraniol; MPTP; Neuroprotective; Parkinson’s disease