Multiplex PCR assay for unequivocal differentiation of Actinobacillus pleuropneumoniae serovars 1 to 3, 5 to 8, 10, and 12.
An improved multiplex PCR, using redesigned primers targeting the serovar 3 capsule locus, which differentiates serovars 3, 6, and 8 Actinobacillus pleuropneumoniae isolates, is described. The new primers eliminate an aberrant serovar 3-indicative amplicon found in some serovar 6 clinical isolates. Furthermore, we have developed a new multiplex PCR for the detection of serovars 1 to 3, 5 to 8, 10, and 12 along with apxIV, thus extending the utility of this diagnostic PCR to cover a broader range of isolates.
Section of Paediatrics, Department of Medicine, Imperial College London, St. Mary's Campus, London, United Kingdom.
Janine T
JT
Bossé
Section of Paediatrics, Department of Medicine, Imperial College London, St. Mary's Campus, London, United Kingdom.
Yanwen
Y
Li
Norwegian Veterinary Institute, Oslo, Norway.
Øystein
Ø
Angen
Department of Veterinary Medicine, University of Cambridge, Cambridge, United Kingdom.
Lucy A
LA
Weinert
Department of Veterinary Medicine, University of Cambridge, Cambridge, United Kingdom.
Roy R
RR
Chaudhuri
The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, United Kingdom.
Matthew T
MT
Holden
Animal Health Veterinary Laboratories Agency (AHVLA), Rougham Hill, Suffolk, United Kingdom.
Susanna M
SM
Williamson
The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, United Kingdom.
Duncan J
DJ
Maskell
The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, United Kingdom.
Alexander W
AW
Tucker
Department of Pathogen Molecular Biology, London School of Hygiene and Tropical Medicine, London, United Kingdom.
Brendan W
BW
Wren
Department of Pathology and Pathogen Biology, The Royal Veterinary College, Hawkshead Campus, Hertfordshire, United Kingdom.
Andrew N
AN
Rycroft
Section of Paediatrics, Department of Medicine, Imperial College London, St. Mary's Campus, London, United Kingdom p.langford@imperial.ac.uk.
Paul R
PR
Langford
D000189:Actinobacillus Infections / Q000382:microbiology; D016977:Actinobacillus pleuropneumoniae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D000818:Animals; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA; D065288:Serogroup
D017931:DNA Primers; D004269:DNA, Bacterial