Tyrosine phosphorylation of β-catenin affects its subcellular localization and transcriptional activity of β-catenin in Hela and Bcap-37 cells.
In order to investigate the relationship between tyrosine phosphorylation of β-catenin and transcriptional activity of β-catenin in Hela and Bcap-37 cells, genistein (a tyrosine kinase inhibitor) was used to inhibit tyrosine phosphorylation in cells. Our results showed the total β-catenin protein levels were mainly equal in Hela, Bcap-37 and HK-2 cells, β-catenin was mainly present in nucleus in Hela and Bcap-37cells, while in HK-2 cell β-catenin was mainly located in cytoplasm. Genistein could inhibit tyrosine phosphorylation of β-catenin and downregulate nuclear β-catenin expression in Hela and Bcap-37 cells. In addition, genistein suppressed Ki-67 promoter activity and Ki-67 protein level, thus promoted cell apoptosis. Furthermore, β-catenin could increase the Ki-67 promoter activity in Hela and Bcap-37 cells. From these findings we conclude that tyrosine phosphorylation of β-catenin can regulate the cellular distribution of β-catenin and affect the transcriptional activity of β-catenin.
Jiangsu Key Laboratory of Biological Cancer Therapy, Xuzhou Medical College, 84 West Huai-hai Road, Xuzhou 221002, Jiangsu, China.
He-Ya
HY
Qian
Jiangsu Key Laboratory of Biological Cancer Therapy, Xuzhou Medical College, 84 West Huai-hai Road, Xuzhou 221002, Jiangsu, China.
Ding-Guo
DG
Zhang
Jiangsu Key Laboratory of Biological Cancer Therapy, Xuzhou Medical College, 84 West Huai-hai Road, Xuzhou 221002, Jiangsu, China.
Hong-Wei
HW
Wang
Jiangsu Key Laboratory of Biological Cancer Therapy, Xuzhou Medical College, 84 West Huai-hai Road, Xuzhou 221002, Jiangsu, China. Electronic address: dspei@xzmc.edu.cn.
Dong-Sheng
DS
Pei
Jiangsu Key Laboratory of Biological Cancer Therapy, Xuzhou Medical College, 84 West Huai-hai Road, Xuzhou 221002, Jiangsu, China; Center of Clinical Oncology, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, China. Electronic address: jnzheng@xzmc.edu.cn.
Jun-Nian
JN
Zheng
D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D019833:Genistein / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D015394:Molecular Structure; D010766:Phosphorylation / Q000187:drug effects; D013329:Structure-Activity Relationship; D014443:Tyrosine / Q000037:antagonists & inhibitors* / Q000378:metabolism; D051176:beta Catenin / Q000037:antagonists & inhibitors* / Q000378:metabolism
D000970:Antineoplastic Agents; D051176:beta Catenin; D014443:Tyrosine; D019833:Genistein
Genistein; Ki-67; Tyrosine phosphorylation; β-Catenin