Effects of supplementation with different Mg salts in cells: is there a clue?
The differing bioavailability of magnesium salts remains an open question, both at the cellular and systemic level. However, this issue is relevant for identifying the most effective magnesium supplement. We compared the effects of three widely used magnesium salts: MgSO4, MgCl2 and Mg pidolate, on the proliferation of four human cell types: promyelocytic leukaemia HL60, osteoblast-like Saos-2 and U-2 OS, and endothelial cells from the umbilical vein. The three magnesium salts had no effect on endothelial and leukemic cell growth, but magnesium pidolate impaired cell growth in osteoblast-like cells. In particular, in Saos-2 cells, 1 mM pidolate induced a slight accumulation of cells in the G0/G1 phase of the cell cycle and, in parallel, an early rise in intracellular calcium and a late decrease in intracellular magnesium content. Interestingly, when cultured in 5 mM magnesium pidolate, Saos-2 cells grew as fast as the controls. Moreover, intracellular magnesium and calcium concentrations did not vary. These results suggest a lower bioavailability of magnesium pidolate in osteoblast-like cells.
Dipartimento di Farmacia e Biotecnologie, Università di Bologna, Via San Donato 19/2, 40127 Bologna, Italy, Istituto Nazionale Biostrutture e Biosistemi, Consorzio Interuniversitario, Via delle Medaglie D'oro, 305, 00136 Roma, Italy.
Giovanna
G
Farruggia
Dipartimento di Scienze Biomediche e Cliniche L. Sacco, Università di Milano, Via Gian Battista Grassi, 74, 20157 Milano, Italy.
Sara
S
Castiglioni
Dipartimento di Farmacia e Biotecnologie, Università di Bologna, Via San Donato 19/2, 40127 Bologna, Italy.
Azzurra
A
Sargenti
Dipartimento di Scienze della Vita, Università di Modena e Reggio Emilia, via Giuseppe Campi, 183, 41125 Modena, Italy.
Chiara
C
Marraccini
Dipartimento di Scienze Biomediche e Cliniche L. Sacco, Università di Milano, Via Gian Battista Grassi, 74, 20157 Milano, Italy.
Alessandra
A
Cazzaniga
Dipartimento di Farmacia e Biotecnologie, Università di Bologna, Via San Donato 19/2, 40127 Bologna, Italy.
Lucia
L
Merolle
Dipartimento di Farmacia e Biotecnologie, Università di Bologna, Via San Donato 19/2, 40127 Bologna, Italy, Istituto Nazionale Biostrutture e Biosistemi, Consorzio Interuniversitario, Via delle Medaglie D'oro, 305, 00136 Roma, Italy.
Stefano
S
Iotti
Dipartimento di Farmacia e Biotecnologie, Università di Bologna, Via San Donato 19/2, 40127 Bologna, Italy.
Concettina
C
Cappadone
Dipartimento di Scienze Biomediche e Cliniche L. Sacco, Università di Milano, Via Gian Battista Grassi, 74, 20157 Milano, Italy.
Jeanette Anne Marie
JA
Maier
D001682:Biological Availability; D002118:Calcium / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D019587:Dietary Supplements; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008274:Magnesium / Q000378:metabolism; D015636:Magnesium Chloride / Q000737:chemistry / Q000494:pharmacology*; D008278:Magnesium Sulfate / Q000737:chemistry / Q000494:pharmacology*; D011761:Pyrrolidonecarboxylic Acid / Q000737:chemistry / Q000494:pharmacology*; D012492:Salts / Q000737:chemistry / Q000494:pharmacology; D013329:Structure-Activity Relationship
D012492:Salts; D015636:Magnesium Chloride; D008278:Magnesium Sulfate; D008274:Magnesium; D002118:Calcium; D011761:Pyrrolidonecarboxylic Acid
cell proliferation; intracellular calcium; intracellular magnesium; magnesium pidolate