A sensitive enzyme-linked immunosorbent assay amplified by biotin-streptavidin system for detecting non-steroidal anti-inflammatory drug ketoprofen.
A sensitive biotin-streptavidin-amplified enzyme-linked immunosorbent assay (BA-ELISA) method was developed for detecting non-steroidal anti-inflammatory drug ketoprofen. Compared with traditional ELISA method, the sensitivity of proposed immunoassay was enhanced by the biotin-streptavidin system. Under the optimal condition, the median inhibitory concentration (IC50) was 0.25 ng mL(-1), with minor cross-reactivity to a number of structural analogs. This developed assay was successfully applied to detect the ketoprofen residues in different fish samples, and good recoveries (72.6-105.5%) were obtained. The results indicated that this immunoassay method could specifically detect trace ketoprofen residues and could be widely used for routine monitoring of food samples.
a Institute of Environmental Monitoring and Evaluation, School of Environment Science and Technology , Shanghai Jiao Tong University , Shanghai , China.
Dan
D
Bu
Hui S
HS
Zhuang
Guang X
GX
Yang
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis*; D017756:Aquaculture; D001710:Biotin; D002347:Carps; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination; D020128:Inhibitory Concentration 50; D007660:Ketoprofen / Q000032:analysis*; D057230:Limit of Detection; D010473:Perciformes; D011817:Rabbits; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D019809:Streptavidin
D000894:Anti-Inflammatory Agents, Non-Steroidal; D001710:Biotin; D019809:Streptavidin; D007660:Ketoprofen
Biotin–streptavidin; ELISA; PPCPs; fish samples; ketoprofen