Metabolomic profiling of anionic metabolites in head and neck cancer cells by capillary ion chromatography with Orbitrap mass spectrometry.
A highly sensitive platform coupling capillary ion chromatography (Cap IC) with Q Exactive mass spectrometer has been developed for metabolic profiling of head and neck squamous cell carcinoma (HNSCC) cells. The Cap IC allowed an excellent separation of anionic polar metabolites, and the sensitivities increased by up to 100-fold compared to reversed-phase liquid chromatography and hydrophilic interaction chromatography performed at either high- or capillary-flow rates. The detection limits for a panel of standard metabolites were between 0.04 to 0.5 nmol/L (0.2 to 3.4 fmol) at a signal-to-noise ratio of 3. This platform was applied to an untargeted metabolomic analysis of head and neck cancer cells and stem-like cancer cells. Differential metabolomics analysis identified significant changes in energy metabolism pathways (e.g., glycolysis and tricarboxylic acid cycle). These experiments demonstrate Cap IC/MS as a powerful metabolomics tool by providing enhanced separation and sensitivity of polar metabolites combined with high resolution and accurate mass measurement (HR/AM) capabilities to differentiate isobaric metabolites.
Thermo Fisher Scientific, Inc., 355 River Oaks Parkway, San Jose, California 95134, United States.
Junhua
J
Wang
Terri T
TT
Christison
Kaori
K
Misuno
Linda
L
Lopez
Andreas F
AF
Huhmer
Yingying
Y
Huang
Shen
S
Hu
D000838:Anions / Q000737:chemistry*; D053801:Capillary Electrochromatography; D045744:Cell Line, Tumor; D006258:Head and Neck Neoplasms / Q000737:chemistry*; D006801:Humans; D013058:Mass Spectrometry; D055432:Metabolomics / Q000379:methods*; D014411:Neoplastic Stem Cells / Q000737:chemistry; D034741:RNA, Small Interfering / Q000494:pharmacology; D055751:SOXC Transcription Factors / Q000737:chemistry / Q000235:genetics
D000838:Anions; D034741:RNA, Small Interfering; C097473:SOX11 protein, human; D055751:SOXC Transcription Factors