Viral load detection using dried blood spots in a cohort of HIV-1-infected children in Uganda: correlations with clinical and immunological criteria for treatment failure.
Correlations between clinical/immunological treatment failure and viral load (VL) detected by dried blood spot (DBS) sampling were explored in HIV-1-infected children in Uganda. Of 104 children on combined antiretroviral treatment (cART), 12.5% experienced clinical and/or immunological failure, while 28.8%, 44.2%, and 26.9% had VLs of <1,000, 1,000 to 5,000, and >5,000 copies/ml, respectively. Clinical/immunological failure poorly predicted virological failure.
Department of Pediatrics, University of Padua, Padua, Italy paolacoste@gmail.com.
Paola
P
Costenaro
Department of Pediatrics, University of Padua, Padua, Italy.
Rebecca
R
Lundin
Department of Surgery, Oncology and Gastroenterology, Section of Oncology and Immunology, AIDS Reference Center, University of Padua, Padua, Italy.
Maria Raffaella
MR
Petrara
Department of Pediatrics, University of Padua, Padua, Italy.
Martina
M
Penazzato
Department of Pediatrics, University of Padua, Padua, Italy.
William
W
Massavon
Nsambya Home Care Department of St. Raphael of St. Francis Nsambya Hospital, Kampala, Uganda.
Susan
S
Kizito
Nsambya Home Care Department of St. Raphael of St. Francis Nsambya Hospital, Kampala, Uganda.
Sandra Monica
SM
Nabachwa
Nsambya Home Care Department of St. Raphael of St. Francis Nsambya Hospital, Kampala, Uganda.
Maria
M
Nannyonga Musoke
Nsambya Home Care Department of St. Raphael of St. Francis Nsambya Hospital, Kampala, Uganda.
Charles
C
Namisi
Department of Pediatrics, University of Padua, Padua, Italy.
Erika
E
Morelli
Department of Pediatrics, University of Padua, Padua, Italy.
Davide
D
Bilardi
Associazione Casa Accoglienza alla Vita Padre Angelo, Trento, Italy.
Antonio
A
Mazza
Department of Surgery, Oncology and Gastroenterology, Section of Oncology and Immunology, AIDS Reference Center, University of Padua, Padua, Italy.
Marisa
M
Zanchetta
Department of Pediatrics, University of Padua, Padua, Italy.
Carlo
C
Giaquinto
Department of Surgery, Oncology and Gastroenterology, Section of Oncology and Immunology, AIDS Reference Center, University of Padua, Padua, Italy Istituto Oncologico Veneto (IOV)-IRCCS, Padua, Italy.
Anita
A
De Rossi
D044966:Anti-Retroviral Agents / Q000627:therapeutic use; D023241:Antiretroviral Therapy, Highly Active; D001769:Blood / Q000821:virology*; D002648:Child; D002675:Child, Preschool; D015331:Cohort Studies; D003890:Desiccation / Q000379:methods*; D005260:Female; D015658:HIV Infections / Q000175:diagnosis / Q000188:drug therapy / Q000276:immunology / Q000821:virology*; D015497:HIV-1 / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000379:methods*; D017211:Treatment Failure; D014454:Uganda; D019562:Viral Load / Q000379:methods*
D044966:Anti-Retroviral Agents