In vivo neuronal action potential recordings via three-dimensional microscale needle-electrode arrays.
Very fine needle-electrode arrays potentially offer both low invasiveness and high spatial resolution of electrophysiological neuronal recordings in vivo. Herein we report the penetrating and recording capabilities of silicon-growth-based three-dimensional microscale-diameter needle-electrodes arrays. The fabricated needles exhibit a circular-cone shape with a 3-μm-diameter tip and a 210-μm length. Due to the microscale diameter, our silicon needles are more flexible than other microfabricated silicon needles with larger diameters. Coating the microscale-needle-tip with platinum black results in an impedance of ~600 kΩ in saline with output/input signal amplitude ratios of more than 90% at 40 Hz-10 kHz. The needles can penetrate into the whisker barrel area of a rat's cerebral cortex, and the action potentials recorded from some neurons exhibit peak-to-peak amplitudes of ~300 μVpp. These results demonstrate the feasibility of in vivo neuronal action potential recordings with a microscale needle-electrode array fabricated using silicon growth technology.
Department of Electrical and Electronic Information Engineering, Toyohashi University of Technology, 1-1 Hibarigaoka Tempaku-cho, Toyohashi, Aichi 441-8580, Japan.
Akifumi
A
Fujishiro
National Institute of Advanced Industrial Science and Technology (AIST), AIST Tsukuba Central 6, Higashi, Tsukuba, Ibaraki 305-8566, Japan.
Hidekazu
H
Kaneko
Department of Mechanical Engineering, Toyohashi University of Technology, 1-1 Hibarigaoka Tempaku-cho, Toyohashi, Aichi 441-8580, Japan.
Takahiro
T
Kawashima
1] Department of Electrical and Electronic Information Engineering, Toyohashi University of Technology, 1-1 Hibarigaoka Tempaku-cho, Toyohashi, Aichi 441-8580, Japan [2] Electronics-Inspired Interdisciplinary Research Institute (EIIRIS), Toyohashi University of Technology, 1-1 Hibarigaoka Tempaku-cho, Toyohashi, Aichi 441-8580, Japan.
Makoto
M
Ishida
Department of Electrical and Electronic Information Engineering, Toyohashi University of Technology, 1-1 Hibarigaoka Tempaku-cho, Toyohashi, Aichi 441-8580, Japan.
Takeshi
T
Kawano
D000200:Action Potentials / Q000502:physiology*; D000818:Animals; D002540:Cerebral Cortex; D017097:Electric Impedance; D008297:Male; D008839:Microelectrodes; D009339:Needles; D009474:Neurons / Q000502:physiology*; D051381:Rats; D012825:Silicon
D012825:Silicon