Lowenstein-Jensen selective medium for reducing contamination in Mycobacterium tuberculosis culture.
We compared Mycobacterium tuberculosis sputum culture recovery and contamination rates between Lowenstein-Jensen medium (LJ) containing the following decontaminants and LJ alone: (i) PANTA (n = 299), (ii) Selectatab-MB (n = 299), and (iii) penicillin G (n = 234). The contamination rate for LJ alone was approximately 31%, versus 5.0% for PANTA-containing, 2% for Selectatab-containing, and 9% for penicillin-containing media (P < 0.001). M. tuberculosis isolation rates were 9.8%, 17%, 18%, and 12% for standard LJ, PANTA, Selectatab, and penicillin cultures, respectively.
Epicentre Mbarara, Mbarara, Uganda epi-ug-labmanager@epicentre.msf.org yap.boum@epicentre.msf.org.
Kennedy
K
Kassaza
Epicentre Mbarara, Mbarara, Uganda.
Patrick
P
Orikiriza
Epicentre, Paris, France.
Augusto
A
Llosa
Mbarara University of Science and Technology, Mbarara, Uganda.
Joel
J
Bazira
Epicentre Mbarara, Mbarara, Uganda.
Dan
D
Nyehangane
Epicentre, Paris, France.
Anne-Laure
AL
Page
Epicentre Mbarara, Mbarara, Uganda Mbarara University of Science and Technology, Mbarara, Uganda epi-ug-labmanager@epicentre.msf.org yap.boum@epicentre.msf.org.
Yap
Y
Boum
D001431:Bacteriological Techniques / Q000379:methods*; D003470:Culture Media / Q000737:chemistry*; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000254:growth & development* / Q000302:isolation & purification*; D012641:Selection, Genetic; D013183:Sputum / Q000382:microbiology; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis
D003470:Culture Media