Carbon nanotubes altering specificity of repeated PCR and DNA integrity properties.
We have investigated the impact of aqueous suspension of single-walled carbon nanotubes (SWCNTs) and multi-walled carbon nanotubes (MWCNTs) on performance of repeated PCR, and discussed their thermal impact on DNA characteristics. Both SWCNTs and MWCNTs are found significantly enhancing the specificity of the repeated PCR and capable of inhibition of long DNA thermal degradation. SWCNTs performed a better specificity in repeated PCR than MWCNTs did. MWCNT-DNA binding was more favorable for protecting long DNA from thermal degradation than SWCNT-DNA binding. The results suggested that CNTs are very useful in repeated PCR working on limited amount of DNA resources.
Xiongwei
X
Du
Hongjie
H
An
Bo
B
Jin
Liangyu
L
Meng
Qingdai
Q
Liu
D004247:DNA / Q000737:chemistry* / Q000235:genetics*; D008422:Materials Testing; D037742:Nanotubes, Carbon / Q000737:chemistry*; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D037742:Nanotubes, Carbon; D004247:DNA