Sequential sludge digestion after diverse pre-treatment conditions: sludge removal, methane production and microbial community changes.
A lab-scale sequential sludge digestion process which consists of a mesophilic anaerobic digester (MAD) and a thermophilic aerobic digester (TAD) was developed. Thermal, thermal-alkaline and long-term alkaline pre-treatments were applied to the feed sludge to examine their effects on sludge removal and methane production. Especially after thermal-alkaline pre-treatment, high COD removal was maintained; methane production rate was also drastically increased by improving the hydrolysis step of sludge degradation. Polymerase chain reaction-denaturing gel gradient electrophoresis indicated that bacterial communities were represented by three phyla (Firmicutes, Proteobacteria, Actinobacteria) and that Clostridium straminisolvens was the major bacterial species in MAD. Quantitative real-time PCR results indicated that Methanosaeta concilli was the major archaeal species in MAD, and that Ureibacillus sp. was the most abundant bacterial species in TAD.
Department of Chemical Engineering, Pohang University of Science and Technology, San 31, Hyoja-Dong, Pohang, Kyungbuk, Republic of Korea.
Sang Kyu
SK
Park
School of Environmental Science and Engineering, Pohang University of Science and Technology, San 31, Hyoja-Dong, Pohang, Kyungbuk, Republic of Korea.
Hyun Min
HM
Jang
School of Environmental Science and Engineering, Pohang University of Science and Technology, San 31, Hyoja-Dong, Pohang, Kyungbuk, Republic of Korea.
Jeong Hyub
JH
Ha
Division of Advanced Nuclear Engineering, Pohang University of Science and Technology, San 31, Hyoja-Dong, Pohang, Kyungbuk, Republic of Korea. Electronic address: jmpark@postech.ac.kr.
Jong Moon
JM
Park
D000332:Aerobiosis; D000693:Anaerobiosis; D001105:Archaea / Q000235:genetics / Q000254:growth & development*; D001419:Bacteria / Q000235:genetics / Q000254:growth & development*; D001426:Bacterial Proteins / Q000032:analysis; D001673:Biodegradation, Environmental; D057919:Biological Oxygen Demand Analysis; D019149:Bioreactors / Q000382:microbiology; D002241:Carbohydrates / Q000032:analysis; D058645:Denaturing Gradient Gel Electrophoresis; D006863:Hydrogen-Ion Concentration; D008697:Methane / Q000096:biosynthesis*; D009584:Nitrogen / Q000032:analysis; D010100:Oxygen / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D012722:Sewage / Q000382:microbiology*; D014835:Volatilization; D014865:Waste Disposal, Fluid; D018508:Water Purification / Q000379:methods*
D001426:Bacterial Proteins; D002241:Carbohydrates; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D009584:Nitrogen; D008697:Methane; D010100:Oxygen
Methane production; Microbial community; Sequential digestion; Sludge pre-treatment; Sludge reduction