Selective DNA-binding by designed bisbenzamidine-homeodomain chimeras.
We report the construction of conjugates between three variants of the helix 3 region of a Q50K engrailed homeodomain and bisbenzamidine minor-groove DNA binders. The hybrid featuring the sequence of the native protein failed to bind to DNA; however, modifications that increased the α-helical folding propensity of the peptide allowed specific DNA binding by a bipartite (major/minor groove) interaction.
Centro Singular de Investigación en Química Biolóxica e Materiais Moleculares (CIQUS) and Departamento de Química Orgánica, Universidade de Santiago de Compostela, Jenaro de la Fuente sn, 15782 Santiago de Compostela (Spain).
Jesús
J
Mosquera
Jéssica
J
Rodríguez
M Eugenio
ME
Vázquez
José L
JL
Mascareñas
D001550:Benzamidines / Q000737:chemistry*; D001665:Binding Sites; D004247:DNA / Q000737:chemistry*; D018398:Homeodomain Proteins / Q000737:chemistry*; D008958:Models, Molecular; D015394:Molecular Structure; D017433:Protein Structure, Secondary
D001550:Benzamidines; D018398:Homeodomain Proteins; D004247:DNA
DNA recognition; molecular recognition; oligonucleotides; peptides; supramolecular chemistry