Influence of physical and mechanical properties of amphiphilic biosynthetic hydrogels on long-term cell viability.
Maintaining the mechanical properties of biofunctional hydrogels of natural resources for tissue engineering and biomedical applications for an intended period of duration is a challenge. Though anionic polysaccharide alginate has been hailed for its excellent biomimetic characters for tissue engineering, it usually fails in load bearing and other dynamic mechanical environment. In this paper this issue was addressed by copolymerizing alginate with the biocompatible and mechanically robust synthetic biodegradable polyester and crosslinking with polyethylene glycol diacrylate (PEGDA) and vinyl co-monomers, 2-hydroxy ethyl methacrylate (HEMA), methyl methacrylate (MMA) and N N׳ methylene bis acrylamide (NMBA) to form three hydrogels. All three hydrogels were amphiphilic, hemocompatible and non-cytotoxic. These hydrogels exhibited appreciable water holding capacity. Comparatively, hydrogel prepared with PEGDA-NMBA crosslinkers displayed larger pore size, increased crosslinking, higher tensile strength and controlled degradation. With appreciable swelling and EWC, this hydrogel elicited better biological responses with long-term cell viability for cardiac tissue engineering.
Sree Chitra Tirunal Institute for Medical Sciences and Technology, Polymer Science Division, BMT Wing, Thiruvananthapuram 695012, Kerala State, India.
Finosh Gnanaprakasam
FG
Thankam
Sree Chitra Tirunal Institute for Medical Sciences and Technology, Polymer Science Division, BMT Wing, Thiruvananthapuram 695012, Kerala State, India. Electronic address: mjayabalan52@gmail.com.
Jayabalan
J
Muthu
D000178:Acrylamides / Q000737:chemistry; D000327:Adsorption; D000464:Alginates / Q000737:chemistry; D000818:Animals; D001672:Biocompatible Materials; D032701:Biomimetics; D001792:Blood Platelets / Q000166:cytology*; D002460:Cell Line; D002470:Cell Survival; D003432:Cross-Linking Reagents / Q000737:chemistry; D004912:Erythrocytes / Q000166:cytology*; D005347:Fibroblasts / Q000166:cytology; D020100:Hydrogels / Q000737:chemistry*; D008422:Materials Testing; D008689:Methacrylates / Q000737:chemistry; D020366:Methylmethacrylate / Q000737:chemistry; D051379:Mice; D009206:Myocardium / Q000473:pathology; D010973:Platelet Adhesiveness; D011091:Polyesters / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D013314:Stress, Mechanical; D013718:Tensile Strength; D023822:Tissue Engineering / Q000379:methods; D014867:Water / Q000737:chemistry
D000178:Acrylamides; D000464:Alginates; D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D020100:Hydrogels; D008689:Methacrylates; D011091:Polyesters; C437167:poly(ethylene glycol)diacrylate; D014867:Water; D020366:Methylmethacrylate; D011092:Polyethylene Glycols; C005044:hydroxyethyl methacrylate; C021221:N,N'-methylenebisacrylamide
Amphiphilicity; Biosynthetic hydrogels; Cross linking density; Long term viability; Mechanical properties