Combination of conventional blood cultures and the SeptiFast molecular test in patients with suspected sepsis for the identification of bloodstream pathogens.
We evaluated performances of the molecular test SeptiFast (SF) for the detection of agents of bloodstream infection (BSI) in patients with suspected sepsis, the majority of them under antibiotic treatment and at high prevalence of HIV-1 infection (10.5%). Matched SF and blood culture (BC) samples (n=1186) from 1024 patients were studied. Two hundred fifty-one episodes of BSI out of 1144 were identified with the combined methods (22%). SF identified more episodes of BSI than BC: 206 versus 176 (χ(2)=7.008, P=0.0081) and a significantly higher number of Gram-negative bacteria than BC (77 versus 53, χ(2)=9.12; P=0.0025), as well as of polymicrobial infections (χ(2)=4.50, P=0.0339). In conclusion, SF combined with BC improved the diagnosis of sepsis, especially in immunocompromised patients.
Laboratory of Microbiology and Virology, Department of Infectious Diseases, Amedeo di Savoia Hospital, 10149 Turin, Italy. Electronic address: elisa.burdino@libero.it.
Elisa
E
Burdino
Laboratory of Microbiology and Virology, Department of Infectious Diseases, Amedeo di Savoia Hospital, 10149 Turin, Italy.
Tina
T
Ruggiero
Laboratory of Microbiology and Virology, Department of Infectious Diseases, Amedeo di Savoia Hospital, 10149 Turin, Italy.
Tiziano
T
Allice
Laboratory of Microbiology and Virology, Department of Infectious Diseases, Amedeo di Savoia Hospital, 10149 Turin, Italy.
Maria Grazia
MG
Milia
Laboratory of Microbiology and Virology, Department of Infectious Diseases, Amedeo di Savoia Hospital, 10149 Turin, Italy.
Gabriella
G
Gregori
Laboratory of Microbiology and Virology, Department of Infectious Diseases, Amedeo di Savoia Hospital, 10149 Turin, Italy.
Rosangela
R
Milano
Laboratory of Microbiology and Virology, Department of Infectious Diseases, Amedeo di Savoia Hospital, 10149 Turin, Italy.
Francesco
F
Cerutti
Department of Infectious Diseases, Amedeo di Savoia Hospital, University of Turin, 10149 Turin, Italy.
Francesco Giuseppe
FG
De Rosa
Emergency Department and Intensive Care Unit, Maria Vittoria Hospital, 10149 Turin, Italy.
Emilpaolo
E
Manno
Department of Infectious Diseases, Amedeo di Savoia Hospital, University of Turin, 10149 Turin, Italy.
Pietro
P
Caramello
Department of Infectious Diseases, Amedeo di Savoia Hospital, University of Turin, 10149 Turin, Italy.
Giovanni
G
Di Perri
Laboratory of Microbiology and Virology, Department of Infectious Diseases, Amedeo di Savoia Hospital, 10149 Turin, Italy.
Valeria
V
Ghisetti
D000328:Adult; D001419:Bacteria / Q000145:classification / Q000302:isolation & purification; D001769:Blood / Q000382:microbiology*; D005658:Fungi / Q000145:classification / Q000302:isolation & purification; D006801:Humans; D008828:Microbiological Techniques / Q000379:methods*; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D012680:Sensitivity and Specificity; D018805:Sepsis / Q000175:diagnosis* / Q000382:microbiology*
Blood culture; Bloodstream infection; Sepsis; SeptiFast