Expression of microRNA-1, microRNA-133a and Hand2 protein in cultured embryonic rat cardiomyocytes.
In this study, we investigated the expression of the pathway, SRF-microRNA-1/microRNA-133a-Hand2, in the Wistar rat embryonic ventricular cardiomyocytes under conventional monolayer culture. The morphological observation of the cultured cardiomyocytes and the mRNA expression levels of three vital constituent proteins, MLC-2v, N-cadherin, and connexin43, demonstrated the immaturity of these cultured cells, which was featured by less myofibril density, immature sarcomeric structure, and significantly lower mRNA expression of the three constituent proteins than those in neonatal ventricular samples. More importantly, results in this study suggest that the change of SRF-microRNA-1/microRNA-133a-Hand2 pathway results into the attenuation of the Hand2 repression in cultured cardiomyocytes. These outcomes are valuable to understand the cellular state as embryonic cardiomyocytes to be in vitro model and might be useful for the assessment of engineered cardiac tissue and cardiac differentiation of stem cells.
Graduate School of Science and Engineering, Yamagata University, Yonezawa-shi, Japan, zhgfeng@yz.yamagata-u.ac.jp.
Zhonggang
Z
Feng
Rie
R
Takahashi
Takao
T
Nakamura
Daisuke
D
Sato
Nobuyuki
N
Shirasawa
Ayako
A
Nakayama
Sakura
S
Kurashige
Tadashi
T
Kosawada
Tatsuo
T
Kitajima
Mitsuo
M
Umezu
D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000096:biosynthesis*; D015153:Blotting, Western; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D015870:Gene Expression / Q000502:physiology; D035683:MicroRNAs / Q000096:biosynthesis*; D032383:Myocytes, Cardiac / Q000378:metabolism* / Q000648:ultrastructure; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats / Q000196:embryology; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction
D051792:Basic Helix-Loop-Helix Proteins; D035683:MicroRNAs; D012333:RNA, Messenger; C498767:Hand2 protein, rat; C542191:MIRN1 microRNA, rat; C549446:MIRN133 microRNA, rat