Basic study of soft tissue augmentation by adipose-inductive biomaterial.
Reconstructive surgery for tumor resection, trauma, and congenital anomaly involves volume augmentation with autologous tissue transfer. However, a healthy region is damaged as a donor site, and the autologous tissue is transferred like a patchwork to the recipient site. We have attempted to induce adipogenesis activity in artificial biomaterial that is injectable with an injection needle for soft tissue augmentation. First of all, the optimal dose of pioglitazone hydrochloride was examined with adipo-precursor cells in terms of the proliferator-activated receptor-γ mRNA expression levels affected by reagent in vitro. Then, salmon collagen with pioglitazone was adjusted in terms of the dose and the salmon collagen was injected into mouse back using an injection needle in vivo. At 4 weeks after implantation, the pioglitazone collagen gel was substituted by mature adipocytes in comparison with the case for control collagen gel without pioglitazone. These results are indicative of the possibility of promoting adipogenesis using collagen with pioglitazone as an adipose-inductive substance.
Department of Plastic and Reconstructive Surgery, School of Medicine, Keio University, Tokyo, Japan.
Masaki
M
Yazawa
Taisuke
T
Mori
Yasuhide
Y
Nakayama
Kazuo
K
Kishi
D017667:Adipocytes / Q000166:cytology / Q000378:metabolism*; D050156:Adipogenesis / Q000187:drug effects*; D000818:Animals; D002478:Cells, Cultured; D003094:Collagen / Q000494:pharmacology*; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D051379:Mice; D000077205:Pioglitazone; D012474:Salmon; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D045162:Thiazolidinediones / Q000494:pharmacology*
D007004:Hypoglycemic Agents; D045162:Thiazolidinediones; D003094:Collagen; D000077205:Pioglitazone
adipose; augmentation; biomaterial; soft tissue