ReadXplorer--visualization and analysis of mapped sequences.
BACKGROUND
Fast algorithms and well-arranged visualizations are required for the comprehensive analysis of the ever-growing size of genomic and transcriptomic next-generation sequencing data.


RESULTS
ReadXplorer is a software offering straightforward visualization and extensive analysis functions for genomic and transcriptomic DNA sequences mapped on a reference. A unique specialty of ReadXplorer is the quality classification of the read mappings. It is incorporated in all analysis functions and displayed in ReadXplorer's various synchronized data viewers for (i) the reference sequence, its base coverage as (ii) normalizable plot and (iii) histogram, (iv) read alignments and (v) read pairs. ReadXplorer's analysis capability covers RNA secondary structure prediction, single nucleotide polymorphism and deletion-insertion polymorphism detection, genomic feature and general coverage analysis. Especially for RNA-Seq data, it offers differential gene expression analysis, transcription start site and operon detection as well as RPKM value and read count calculations. Furthermore, ReadXplorer can combine or superimpose coverage of different datasets.


METHODS
ReadXplorer is available as open-source software at http://www.readxplorer.org along with a detailed manual.
Institute of Medical Microbiology, Justus-Liebig-University, 35392 Giessen, Germany, Faculty of Biology, Institute for Bioinformatics, Center for Biotechnology, Computational Genomics, Center for Biotechnology, Technology Platform Genomics, Center for Biotechnology, Genome Informatics, Faculty of Technology, Bielefeld University, 33615 Bielefeld, Germany and Bioinformatics and Systems Biology, Faculty of Biology and Chemistry, Justus-Liebig-University, 35392 Giessen, Germany.
Rolf
R
Hilker
Institute of Medical Microbiology, Justus-Liebig-University, 35392 Giessen, Germany, Faculty of Biology, Institute for Bioinformatics, Center for Biotechnology, Computational Genomics, Center for Biotechnology, Technology Platform Genomics, Center for Biotechnology, Genome Informatics, Faculty of Technology, Bielefeld University, 33615 Bielefeld, Germany and Bioinformatics and Systems Biology, Faculty of Biology and Chemistry, Justus-Liebig-University, 35392 Giessen, GermanyInstitute of Medical Microbiology, Justus-Liebig-University, 35392 Giessen, Germany, Faculty of Biology, Institute for Bioinformatics, Center for Biotechnology, Computational Genomics, Center for Biotechnology, Technology Platform Genomics, Center for Biotechnology, Genome Informatics, Faculty of Technology, Bielefeld University, 33615 Bielefeld, Germany and Bioinformatics and Systems Biology, Faculty of Biology and Chemistry, Justus-Liebig-University, 35392 Giessen, Germany.
Kai Bernd
KB
Stadermann
Institute of Medical Microbiology, Justus-Liebig-University, 35392 Giessen, Germany, Faculty of Biology, Institute for Bioinformatics, Center for Biotechnology, Computational Genomics, Center for Biotechnology, Technology Platform Genomics, Center for Biotechnology, Genome Informatics, Faculty of Technology, Bielefeld University, 33615 Bielefeld, Germany and Bioinformatics and Systems Biology, Faculty of Biology and Chemistry, Justus-Liebig-University, 35392 Giessen, Germany.
Daniel
D
Doppmeier
Institute of Medical Microbiology, Justus-Liebig-University, 35392 Giessen, Germany, Faculty of Biology, Institute for Bioinformatics, Center for Biotechnology, Computational Genomics, Center for Biotechnology, Technology Platform Genomics, Center for Biotechnology, Genome Informatics, Faculty of Technology, Bielefeld University, 33615 Bielefeld, Germany and Bioinformatics and Systems Biology, Faculty of Biology and Chemistry, Justus-Liebig-University, 35392 Giessen, Germany.
Jörn
J
Kalinowski
Institute of Medical Microbiology, Justus-Liebig-University, 35392 Giessen, Germany, Faculty of Biology, Institute for Bioinformatics, Center for Biotechnology, Computational Genomics, Center for Biotechnology, Technology Platform Genomics, Center for Biotechnology, Genome Informatics, Faculty of Technology, Bielefeld University, 33615 Bielefeld, Germany and Bioinformatics and Systems Biology, Faculty of Biology and Chemistry, Justus-Liebig-University, 35392 Giessen, GermanyInstitute of Medical Microbiology, Justus-Liebig-University, 35392 Giessen, Germany, Faculty of Biology, Institute for Bioinformatics, Center for Biotechnology, Computational Genomics, Center for Biotechnology, Technology Platform Genomics, Center for Biotechnology, Genome Informatics, Faculty of Technology, Bielefeld University, 33615 Bielefeld, Germany and Bioinformatics and Systems Biology, Faculty of Biology and Chemistry, Justus-Liebig-University, 35392 Giessen, Germany.
Jens
J
Stoye
Institute of Medical Microbiology, Justus-Liebig-University, 35392 Giessen, Germany, Faculty of Biology, Institute for Bioinformatics, Center for Biotechnology, Computational Genomics, Center for Biotechnology, Technology Platform Genomics, Center for Biotechnology, Genome Informatics, Faculty of Technology, Bielefeld University, 33615 Bielefeld, Germany and Bioinformatics and Systems Biology, Faculty of Biology and Chemistry, Justus-Liebig-University, 35392 Giessen, Germany.
Jasmin
J
Straube
Institute of Medical Microbiology, Justus-Liebig-University, 35392 Giessen, Germany, Faculty of Biology, Institute for Bioinformatics, Center for Biotechnology, Computational Genomics, Center for Biotechnology, Technology Platform Genomics, Center for Biotechnology, Genome Informatics, Faculty of Technology, Bielefeld University, 33615 Bielefeld, Germany and Bioinformatics and Systems Biology, Faculty of Biology and Chemistry, Justus-Liebig-University, 35392 Giessen, Germany.
Jörn
J
Winnebald
Institute of Medical Microbiology, Justus-Liebig-University, 35392 Giessen, Germany, Faculty of Biology, Institute for Bioinformatics, Center for Biotechnology, Computational Genomics, Center for Biotechnology, Technology Platform Genomics, Center for Biotechnology, Genome Informatics, Faculty of Technology, Bielefeld University, 33615 Bielefeld, Germany and Bioinformatics and Systems Biology, Faculty of Biology and Chemistry, Justus-Liebig-University, 35392 Giessen, Germany.
Alexander
A
Goesmann
D003196:Computer Graphics; D020869:Gene Expression Profiling; D023281:Genomics / Q000379:methods; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D009876:Operon; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D012313:RNA / Q000737:chemistry; D016415:Sequence Alignment / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000379:methods*; D012984:Software; D024363:Transcription Initiation Site
D012313:RNA