In vivo performance of combinations of autograft, demineralized bone matrix, and tricalcium phosphate in a rabbit femoral defect model.
Large bone defects may be treated with autologous or allogeneic bone preparations. Each treatment has advantages and disadvantages; therefore, a clinically viable option for treating large (e.g., gap) bone defects may be a combination of the two. In the present study, bone repair was determined with combinations of autografts, allografts, and synthetic bone grafts using an established rabbit femoral defect model. Bilateral unicortical femoral defects were surgically prepared and treated with combinatorial bone grafts according to one of seven treatment groups. Recipient sites were retrieved at six weeks. Cellular/tissue responses and new bone formation were assessed by histology and histomorphometry. Histological analysis images indicated neither evidence of inflammatory, immune responses, tissue necrosis, nor osteolysis. Data suggested co-integration of implanted agents with host and newly formed bone. Finally, the histomorphometric data suggested that the tricalcium phosphate-based synthetic bone graft substitute allowed new bone formation that was similar to the allograft (i.e., demineralized bone matrix, DBM).
Department of Biomedical Engineering, Bone Tissue Engineering Center, Carnegie Mellon University, Pittsburgh, PA 15219, USA. Department of Bio and Chemical Engineering, Hongik University, Sejong, 339-701 Korea.
Jinku
J
Kim
Sean
S
McBride
David D
DD
Dean
Victor L
VL
Sylvia
Bruce A
BA
Doll
Jeffrey O
JO
Hollinger
D000818:Animals; D018775:Bone Demineralization Technique / Q000379:methods; D001857:Bone Matrix / Q000637:transplantation*; D001861:Bone Regeneration / Q000502:physiology; D018786:Bone Substitutes / Q000627:therapeutic use*; D016025:Bone Transplantation / Q000295:instrumentation* / Q000379:methods; D002113:Calcification, Physiologic; D002130:Calcium Phosphates / Q000737:chemistry*; D003131:Combined Modality Therapy / Q000295:instrumentation / Q000379:methods; D019544:Equipment Failure Analysis; D005260:Female; D005264:Femoral Fractures / Q000473:pathology* / Q000503:physiopathology / Q000628:therapy*; D048091:Guided Tissue Regeneration / Q000295:instrumentation* / Q000379:methods; D008422:Materials Testing; D011474:Prosthesis Design; D011817:Rabbits; D016896:Treatment Outcome
D018786:Bone Substitutes; D002130:Calcium Phosphates; C018392:tricalcium phosphate