Potent airway smooth muscle relaxant effect of cynatratoside B, a steroidal glycoside isolated from Cynanchum stauntonii.
The dried roots of Cynanchum stauntonii in having cough-relieving efficacy are commonly included in traditional antitussive formulas. The active components in a C. stauntonii root extract responsible for airway relaxation were isolated using an ex vivo bioassay-guided fractionation method, in which subfractions were evaluated for their inhibitory effects on the contraction of isolated rat tracheal rings by isometric tension measurements. A steroidal glycoside, cynatratoside B (1), identified by LC-MS and NMR spectroscopic analysis, was shown to have potent inhibition on acetylcholine- and carbachol-induced tracheal contractions. The present data provide scientific evidence to support the traditional use of C. stauntonii as an antitussive herbal medicine.
Institute of Chinese Medicine, The Chinese University of Hong Kong , Shatin, New Territories, Hong Kong, People's Republic of China.
Grace Gar-Lee
GG
Yue
Kar-Man
KM
Chan
Ming-Ho
MH
To
Ling
L
Cheng
Kwok-Pui
KP
Fung
Ping-Chung
PC
Leung
Clara Bik-San
CB
Lau
D000818:Animals; D002217:Carbachol / Q000494:pharmacology; D031105:Cynanchum / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D006723:Hong Kong; D008297:Male; D015394:Molecular Structure; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013256:Steroids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D014132:Trachea / Q000187:drug effects
D006027:Glycosides; D013256:Steroids; C000589833:cynatratoside B; D002217:Carbachol