Evidence for a type III secretion system in Chlamydophila psittaci.
In order to examine the presence of a type III secretion system in Chlamydophila psittaci, we focused on the SctW (CopN) coding region in a locus containing four genes encoding putative products with similarity to chaperones (Sccl), secretion pore components (Cds1 and Cds2) and secreted proteins (CopN) formerly identified in the type III secretion system of other gram-negative bacteria. SctW regulates type III secretion in gram-negative bacteria. SctW expression was examined in C. psittaci infected HeLa cells using SctW-specific polyclonal antibodies in an indirect immunofluorescence staining. SctW expression was detected 29 hours post inoculation and was absent in uninfected control cells. Immunoblotting of whole HeLa cell lysate 72 hours post inoculation with the SctW-specific polyclonal antibody also revealed the presence of SctW. Results demonstrated SctW of C. psittaci to be expressed in at least one stage of the intracellular bacterial life cycle. The present finding may contribute to the future identification of a functional type III secretion apparatus in C. psittaci.
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D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D058947:Bacterial Secretion Systems; D002691:Chlamydophila psittaci / Q000235:genetics / Q000502:physiology*; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D015964:Gene Expression Regulation, Bacterial; D006367:HeLa Cells; D006801:Humans; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011201:Poultry Diseases / Q000382:microbiology*; D009956:Psittacosis / Q000382:microbiology / Q000662:veterinary*; D014422:Turkeys
D001426:Bacterial Proteins; D058947:Bacterial Secretion Systems