Evaluation of the RIDAGENE real-time PCR assay for the detection of GI and GII norovirus.
The current study examined the efficacy of the RIDAGENE norovirus (NoV) real-time polymerase chain reaction assay (R-Biopharm, Darmstadt, Germany) for use in a routine diagnostic laboratory. The RIDAGENE assay had an overall sensitivity of 98% but was more sensitive for GII than GI NoV. The assay had a specificity of 98%. The RIDAGENE assay could detect a variety of GI and GII open reading frame 2 genotypes including GI.1, GI.3, GI.8, GI.13, GII.2, GII.3, GII.4 (including the following variants: 2006b, 2009, 2012, and 3 others that have not been assigned), GII.6, GII.12, and GII.13. The assay did not cross react with a number of gastroenteritis viruses including adenovirus, astrovirus, rotavirus, and sapovirus. The assay was straightforward to perform, and for a run of 50 specimens, a result was obtainable in roughly 4 hours. The RIDAGENE assay can be recommended as a valuable detection method for NoV.
Victorian Infectious Diseases Reference Laboratory, North Melbourne, Victoria, Australia.
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Dunbar
Victorian Infectious Diseases Reference Laboratory, North Melbourne, Victoria, Australia.
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Bruggink
Victorian Infectious Diseases Reference Laboratory, North Melbourne, Victoria, Australia. Electronic address: john.marshall@mh.org.au.
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Marshall
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017250:Caliciviridae Infections / Q000175:diagnosis*; D002648:Child; D002675:Child, Preschool; D005260:Female; D005759:Gastroenteritis / Q000175:diagnosis* / Q000821:virology*; D005838:Genotype; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008875:Middle Aged; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D029322:Norovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D055815:Young Adult
Norovirus; Real-time PCR