[Application of miRNAs in the occurrence and early diagnosis of pancreatic cancer].
OBJECTIVE
To find the potential serum specific miRNAs with diagnostic value in early pancreatic cancer and study the alteration of miRNAs levels in the process of origin and development of pancreatic cancer and discuss the diagnostic value of miRNAs in early pancreatic cancer.


METHODS
DMBA-induced rat model was established. The miRNAs expression profile of early stage was screened out by microarray. And confirmation study was performed.


RESULTS
The 35 and 12 abnormally expressed miRNAs were acquired in pancreatic tissue and blood respectively. There were no significant differences between normal pancreas and pancreatic cancer in expressions of hsa-let-7c, hsa-miR-122-5p, hsa-miR-142-5p, hsa-miR-199a-3p and hsa-miR-451a (P > 0.05).


CONCLUSIONS
miRNAs are the potential biomarkers of early pancreatic cancer. The establishment of the miRNAs expression profile has build the foundation of exploring the molecular mechanism of origin of pancreatic cancer.
Department of General Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing 100730, China.
Shujun
S
Xia
Department of General Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing 100730, China.
Junchao
J
Guo
Department of General Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing 100730, China.
Jian
J
Li
Department of General Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing 100730, China.
Li
L
Zhou
Department of General Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing 100730, China. Email: zhao8028@263.net.
Yupei
Y
Zhao
D000818:Animals; D014408:Biomarkers, Tumor / Q000097:blood / Q000378:metabolism; D004195:Disease Models, Animal; D008297:Male; D035683:MicroRNAs / Q000097:blood / Q000378:metabolism*; D010179:Pancreas / Q000378:metabolism; D010190:Pancreatic Neoplasms / Q000175:diagnosis* / Q000235:genetics; D011379:Prognosis; D051381:Rats; D017207:Rats, Sprague-Dawley; D059467:Transcriptome
D014408:Biomarkers, Tumor; D035683:MicroRNAs