Acetylcholinesterase biosensor for carbaryl detection based on interdigitated array microelectrodes.
In this study, an acetylcholinesterase (AChE) biosensor with superior accuracy and sensitivity was successfully developed based on interdigitated array microelectrodes (IAMs). IAMs have a series of parallel microband electrodes with alternating microbands connected together. Chitosan was used as the enzyme immobilization material, and AChE was used as the model enzyme for carbaryl detection to fabricate AChE biosensor. Electrochemical impedance spectroscopy was used in conjunction with the fabricated biosensor to detect pesticide residues. Based on the inhibition of pesticides on the AChE activity, using carbaryl as model compounds, the biosensor exhibited a wide range, low detection limit, and high stability. Moreover, the biosensor can also be used as a new promising tool for pesticide residue analysis.
School of Agricultural and Food Engineering, Shandong University of Technology, No.12, Zhangzhou Road, Zibo, 255049, Shandong, People's Republic of China.
Zhili
Z
Gong
Yemin
Y
Guo
Xia
X
Sun
Yaoyao
Y
Cao
Xiangyou
X
Wang
D000110:Acetylcholinesterase / Q000378:metabolism*; D015374:Biosensing Techniques; D012721:Carbaryl / Q000032:analysis*; D002800:Cholinesterase Inhibitors / Q000032:analysis*; D057230:Limit of Detection; D008839:Microelectrodes; D013056:Spectrophotometry, Ultraviolet
D002800:Cholinesterase Inhibitors; D000110:Acetylcholinesterase; D012721:Carbaryl