Monocyte subsets in schistosomiasis patients with periportal fibrosis.
A major issue with Schistosoma mansoni infection is the development of periportal fibrosis, which is predominantly caused by the host immune response to egg antigens. Experimental studies have pointed to the participation of monocytes in the pathogenesis of liver fibrosis. The aim of this study was to characterize the subsets of monocytes in individuals with different degrees of periportal fibrosis secondary to schistosomiasis. Monocytes were classified into classical (CD14(++)CD16(-)), intermediate (CD14(++)CD16(+)), and nonclassical (CD14(+)CD16(++)). The expressions of monocyte markers and cytokines were assessed using flow cytometry. The frequency of classical monocytes was higher than the other subsets. The expression of HLA-DR, IL-6, TNF-α, and TGF-β was higher in monocytes from individuals with moderate to severe fibrosis as compared to other groups. Although no differences were observed in receptors expression (IL-4R and IL-10R) between groups of patients, the expression of IL-12 was lower in monocytes from individuals with moderate to severe fibrosis, suggesting a protective role of this cytokine in the development of fibrosis. Our data support the hypothesis that the three different monocyte populations participate in the immunopathogenesis of periportal fibrosis, since they express high levels of proinflammatory and profibrotic cytokines and low levels of regulatory markers.
Serviço de Imunologia, Complexo Hospitalar Universitário Professor Edgard Santos, Universidade Federal da Bahia, Rua João das Botas s/n, Canela, 40110-160 Salvador, BA, Brazil.
Jamille Souza
JS
Fernandes
Serviço de Imunologia, Complexo Hospitalar Universitário Professor Edgard Santos, Universidade Federal da Bahia, Rua João das Botas s/n, Canela, 40110-160 Salvador, BA, Brazil ; Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais (INCT-DT), CNPQ/MCT, Brazil ; Escola Bahiana de Medicina e Saúde Pública, 40050-420 Salvador, BA, Brazil.
Maria Ilma
MI
Araujo
Serviço de Imunologia, Complexo Hospitalar Universitário Professor Edgard Santos, Universidade Federal da Bahia, Rua João das Botas s/n, Canela, 40110-160 Salvador, BA, Brazil.
Diego Mota
DM
Lopes
Serviço de Imunologia, Complexo Hospitalar Universitário Professor Edgard Santos, Universidade Federal da Bahia, Rua João das Botas s/n, Canela, 40110-160 Salvador, BA, Brazil.
Robson da Paixão
Rda P
de Souza
Serviço de Imunologia, Complexo Hospitalar Universitário Professor Edgard Santos, Universidade Federal da Bahia, Rua João das Botas s/n, Canela, 40110-160 Salvador, BA, Brazil ; Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais (INCT-DT), CNPQ/MCT, Brazil ; Escola Bahiana de Medicina e Saúde Pública, 40050-420 Salvador, BA, Brazil.
Edgar M
EM
Carvalho
Serviço de Imunologia, Complexo Hospitalar Universitário Professor Edgard Santos, Universidade Federal da Bahia, Rua João das Botas s/n, Canela, 40110-160 Salvador, BA, Brazil ; Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais (INCT-DT), CNPQ/MCT, Brazil ; Departamento de Análises Clínicas e Toxicológicas, Faculdade de Farmácia, UFBA, 40170-115 Salvador, BA, Brazil.
Luciana Santos
LS
Cardoso
D000328:Adult; D005260:Female; D005434:Flow Cytometry; D058851:GPI-Linked Proteins / Q000378:metabolism; D005786:Gene Expression Regulation; D006684:HLA-DR Antigens / Q000378:metabolism; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D018950:Lipopolysaccharide Receptors / Q000378:metabolism; D008103:Liver Cirrhosis / Q000469:parasitology*; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000166:cytology* / Q000469:parasitology*; D017452:Receptors, IgG / Q000378:metabolism; D053701:Receptors, Interleukin-10 / Q000378:metabolism; D019948:Receptors, Interleukin-4 / Q000378:metabolism; D012552:Schistosomiasis / Q000503:physiopathology*; D016212:Transforming Growth Factor beta / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
C428200:FCGR3B protein, human; D058851:GPI-Linked Proteins; D006684:HLA-DR Antigens; D015850:Interleukin-6; D018950:Lipopolysaccharide Receptors; D017452:Receptors, IgG; D053701:Receptors, Interleukin-10; D019948:Receptors, Interleukin-4; D016212:Transforming Growth Factor beta; D014409:Tumor Necrosis Factor-alpha