Association of TP53 codon 72 and intron 3 16-bp Ins/Del polymorphisms with cervical cancer risk.
Cervical cancer incidence has grown worldwide, with it being a more significant problem in developing countries. Invasive squamous cell cervical cancers are preceded by a long phase of preinvasive disease, known as cervical intraepithelial neoplasia. Cervical cancer can develop when the virus takes advantage of any TP53 gene dysfunction of the host organism. TP53 is responsible for encoding the tumor suppressor p53 phosphoprotein, which helps preserve genome integrity. Currently, many studies have focused on genetic polymorphisms as an important contribution to cancer susceptibility, but few related to cervical intraepithelial neoplasia (CIN). Thus, the present study aimed to see whether patients with suspected CIN had TP53 gene polymorphisms that might have contributed to the development of neoplasia. This study included 133 women who were referred to the Cervical Pathology Clinic of the Maternity School Assis Chateaubriand MEAC for suspected cervical lesions. Polymorphism genotyping was carried out by the PCR-RFLP technique using DNA extracted from patients' blood. The most frequent genotype in both CIN(+) and CIN(-) patients was Arg/Pro TP53 codon 72 and A1A1 for 16-bp Del in intron 3. No risk of cervical cancer was found for the polymorphisms studied. However, a significant association was found when the two polymorphisms were combined: patients with the A1A1/ArgPro genotype were statistically more frequent in the CIN(-) group (p = 0.042), while A2A2-A1A2/ProArg was significantly more frequent in the CIN(+) group. The results of our study suggest that combined analysis of TP53 polymorphisms Arg72Pro and 16-bp Ins/Del may help to monitor the development of CIN in Brazilian women.
Department of Medicine, Federal University of Ceará, Fortaleza, CE, Brazil.
Tatiana Dantas Rodrigues
TD
Laprano
Erika Hardy
EH
Lemos
Lia Moreira Pinto
LM
Cunha
José Eleutério
JE
Júnior
Rosiane Alves
RA
de SousaTeles
Silvia Helena Barem
SH
Rabenhorst
D000293:Adolescent; D000328:Adult; D003062:Codon; D005260:Female; D016158:Genes, p53; D020022:Genetic Predisposition to Disease; D006801:Humans; D007438:Introns; D008875:Middle Aged; D011110:Polymorphism, Genetic; D012306:Risk; D002583:Uterine Cervical Neoplasms / Q000209:etiology / Q000235:genetics*; D002578:Uterine Cervical Dysplasia / Q000235:genetics*
D003062:Codon