Signaling events during macrophage activation with Betula pendula roth pectic polysaccharides.
We studied the effect of two pectic polysaccharides PS-B1-AG and PS-B2-RG that were contained in total polysaccharides extracted from Betula pendula leaves on NO production by mouse macrophages and the contribution of signaling molecules to macrophage activation by the test substances. Unlike the total sample, pectins produced a NO-stimulating effect on macrophages. The effect of PS-B2-RG (10 μg/ml) did not differ from the effect of LPS, while PS-B1-AG produced this effect only in a concentration of 20 μg/ml, which was probably due to differences in the chemical structure of the test substances. The studied pectin polysaccharides activated transcription factor NF-κB, kinases p38 and PI3, and cAMP as a negative regulator. These results indicate that Betula pendula polysaccharides are promising substances for creation of immunomodulating drugs.
Research Institute of Pharmacology, Siberian Division of the Russian Academy of Medical Sciences, Tomsk, Russia.
A A
AA
Ligacheva
M G
MG
Danilets
E S
ES
Trofimova
Y P
YP
Belsky
N V
NV
Belska
G N
GN
Zyuz'kov
V V
VV
Zhdanov
A N
AN
Ivanova
A M
AM
Guriev
M V
MV
Belousov
M S
MS
Yusubov
A M
AM
Dygai
D000818:Animals; D029662:Betula / Q000737:chemistry*; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism; D005260:Female; D007155:Immunologic Factors / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008262:Macrophage Activation / Q000187:drug effects*; D008264:Macrophages / Q000276:immunology / Q000378:metabolism; D008297:Male; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D016328:NF-kappa B / Q000378:metabolism; D009569:Nitric Oxide / Q000096:biosynthesis; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D018515:Plant Leaves / Q000737:chemistry; D011134:Polysaccharides / Q000494:pharmacology*; D015398:Signal Transduction; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
D007155:Immunologic Factors; D008070:Lipopolysaccharides; D016328:NF-kappa B; C000593607:PS-B1-AG polysaccharide; C000593608:PS-B2-RG polysaccharide; D011134:Polysaccharides; D009569:Nitric Oxide; D000242:Cyclic AMP; D019869:Phosphatidylinositol 3-Kinases; D048051:p38 Mitogen-Activated Protein Kinases