Involvement of S. cerevisiae Rpb4 in subset of pathways related to transcription elongation.
Yeast Rpb4, a subunit of RNA pol II is not essential for viability but is involved in multiple cellular phenotypes such as temperature sensitivity, enhanced pseudohyphal morphology, and decreased sporulation. Both in vivo and in vitro studies strongly support involvement of Rpb4 in transcription initiation, while its role in transcription elongation is not entirely consistent. Here we show that Rpb4 is not required for recruitment of RNA pol II on the coding region of YLR454w, a representative long gene. Yet we find strong genetic interaction of rpb4∆ with mutants in many transcription elongation factors such as Paf1, Spt4, Dst1, Elp3 and Rpb9. We demonstrate that, Rpb4 interacts functionally with Paf1 to affect the transcription elongation of the FKS1 gene. Our results suggest that while Rpb4 is not required for general transcription elongation, it could support transcription elongation for specific of class of genes by interaction with other elongation factors.
Dept. of Microbiology and Cell Biology, Indian Institute of Science, Bangalore 560 012, India.
Swati M
SM
Deshpande
Dept. of Microbiology and Cell Biology, Indian Institute of Science, Bangalore 560 012, India.
Parag P
PP
Sadhale
Dept. of Microbiology and Cell Biology, Indian Institute of Science, Bangalore 560 012, India. Electronic address: uvr123@gmail.com.
Usha
U
Vijayraghavan
D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D054714:Echinocandins / Q000235:genetics / Q000378:metabolism; D005964:Glucosyltransferases / Q000235:genetics / Q000378:metabolism; D051548:Histone Acetyltransferases / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D011493:Protein Kinase C / Q000378:metabolism; D012319:RNA Polymerase II / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000502:physiology; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D061805:Transcription Elongation, Genetic; D035602:Transcriptional Elongation Factors / Q000235:genetics / Q000378:metabolism
D004268:DNA-Binding Proteins; C068394:DST1 protein, S cerevisiae; D054714:Echinocandins; D008565:Membrane Proteins; D009687:Nuclear Proteins; C097698:PAF1 protein, S cerevisiae; C080991:SPT4 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D035602:Transcriptional Elongation Factors; C526363:Elp3 protein, S cerevisiae; D051548:Histone Acetyltransferases; D005964:Glucosyltransferases; C091618:FKS1 protein, S cerevisiae; D011493:Protein Kinase C; D012319:RNA Polymerase II; C423309:RPB4 protein, S cerevisiae; C509257:Rpb9 protein, S cerevisiae
Elongation factors; PKC pathway; Rpb4; Synthetic lethality; Transcription elongation