Efficient calcium lactate production by fermentation coupled with crystallization-based in situ product removal.
Lactic acid is a platform chemical with various industrial applications, and its derivative, calcium lactate, is an important food additive. Fermentation coupled with in situ product removal (ISPR) can provide more outputs with high productivity. The method used in this study was based on calcium lactate crystallization. Three cycles of crystallization were performed during the fermentation course using a Bacillus coagulans strain H-1. As compared to fed-batch fermentation, this method showed 1.7 times higher average productivity considering seed culture, with 74.4% more L-lactic acid produced in the fermentation with ISPR. Thus, fermentation coupled with crystallization-based ISPR may be a biotechnological alternative that provides an efficient system for production of calcium lactate or lactic acid.
State Key Laboratory of Microbial Metabolism, and School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China.
Ke
K
Xu
State Key Laboratory of Microbial Metabolism, and School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China. Electronic address: pingxu@sjtu.edu.cn.
Ping
P
Xu
D019149:Bioreactors; D017610:Calcium Compounds / Q000302:isolation & purification / Q000378:metabolism*; D003460:Crystallization; D005285:Fermentation; D007773:Lactates / Q000302:isolation & purification / Q000378:metabolism*
D017610:Calcium Compounds; D007773:Lactates; C110051:calcium lactate
Bacillus coagulans; Calcium lactate; Crystallization; Fermentation