Accurate single-shot quantitative phase imaging of biological specimens with telecentric digital holographic microscopy.
The advantages of using a telecentric imaging system in digital holographic microscopy (DHM) to study biological specimens are highlighted. To this end, the performances of nontelecentric DHM and telecentric DHM are evaluated from the quantitative phase imaging (QPI) point of view. The evaluated stability of the microscope allows single-shot QPI in DHM by using telecentric imaging systems. Quantitative phase maps of a section of the head of the drosophila melanogaster fly and of red blood cells are obtained via single-shot DHM with no numerical postprocessing. With these maps we show that the use of telecentric DHM provides larger field of view for a given magnification and permits more accurate QPI measurements with less number of computational operations.
University of Valencia, 3D Imaging and Display Laboratory, Department of Optics, E-46100 Burjassot, Spain.
Ana
A
Doblas
University of Valencia, 3D Imaging and Display Laboratory, Department of Optics, E-46100 Burjassot, Spain.
Emilio
E
Sánchez-Ortiga
University of Valencia, 3D Imaging and Display Laboratory, Department of Optics, E-46100 Burjassot, Spain.
Manuel
M
Martínez-Corral
University of Valencia, 3D Imaging and Display Laboratory, Department of Optics, E-46100 Burjassot, Spain.
Genaro
G
Saavedra
University of Valencia, 3D Imaging and Display Laboratory, Department of Optics, E-46100 Burjassot, SpainbUniversidad Nacional de Colombia Sede Medellin, School of Physics, A.A. 3840, Medellin 050034, Colombia.
Jorge
J
Garcia-Sucerquia
D000818:Animals; D004331:Drosophila melanogaster; D004912:Erythrocytes / Q000166:cytology; D006257:Head / Q000033:anatomy & histology; D006696:Holography / Q000379:methods*; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008853:Microscopy / Q000379:methods*