Survey on the occurrence of aflatoxins in rice from different provinces of Iran.
Aflatoxins were surveyed in 256 rice samples taken from retail markets in different provinces of Iran during October 2007 and July 2008. A methanol/water (80 : 20, v/v) mixture and an aflatoxin immunoaffinity column (IAC) were used for extraction and clean-up. Mycotoxins were determined using HPLC with fluorescence detection and post-column derivatization using a photo-ionization cell. Levels of contamination ranged 0.0-5.8 ng g(-1) (mean, 1.4 ng g(-1)) and 0.1-6.3 ng g(-1) (mean, 1.6 ng g(-1)) for AFB1 and total aflatoxins, respectively. AFB1 was detected in almost all samples. Results showed that 55 samples (21.5%) were contaminated with more than 2 µg kg(-1) of AFB1, while seven samples (2.7 %) contained more than 4 µg kg(-1) total aflatoxins. The calculated probable daily intake of AFB1 from rice for Iranians ranged 1.4-5.8 ng AFB1 per kg body weight per day for average consumers and, hence. exceeding the estimated provisional maximum tolerable daily intake.
a Department of Food Science and Technology , Science and Research Branch, Islamic Azad University , Tehran , Iran.
Anosheh
A
Rahmani
Farhang
F
Soleimany
Hedayat
H
Hosseini
Leila
L
Nateghi
D016604:Aflatoxin B1 / Q000032:analysis; D000348:Aflatoxins / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004032:Diet; D005506:Food Contamination / Q000032:analysis; D006801:Humans; D007492:Iran; D008452:Maximum Allowable Concentration; D012275:Oryza / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry
D000348:Aflatoxins; D016604:Aflatoxin B1
aflatoxins; cereals; mycotoxins