Thermal expansion of vitrified blood vessels permeated with DP6 and synthetic ice modulators.
This study provides thermal expansion data for blood vessels permeated with the cryoprotective cocktail DP6, when combined with selected synthetic ice modulators (SIMs): 12% polyethylene glycol 400, 6% 1,3-cyclohexanediol, and 6% 2,3-butanediol. The general classification of SIMs includes molecules that modulate ice nucleation and growth, or possess properties of stabilizing the amorphous state, by virtue of their chemical structure and at concentrations that are not explained on a purely colligative basis. The current study is part of an ongoing effort to characterize thermo-mechanical effects on structural integrity of cryopreserved materials, where thermal expansion is the driving mechanism to thermo-mechanical stress. This study focuses on the lower part of the cryogenic temperature range, where the cryoprotective agent (CPA) behaves as a solid for all practical applications. By combining results obtained in the current study with literature data on the thermal expansion in the upper part of the cryogenic temperature range, unified thermal expansion curves are presented.
Biothermal Technology Laboratory, Department of Mechanical Engineering, Carnegie Mellon University, Pittsburgh, PA 15213, United States.
David P
DP
Eisenberg
Biothermal Technology Laboratory, Department of Mechanical Engineering, Carnegie Mellon University, Pittsburgh, PA 15213, United States; Cell and Tissue Systems, Inc., 2231 Technical Parkway, N. Charleston, SC 29406, United States.
Michael J
MJ
Taylor
Biothermal Technology Laboratory, Department of Mechanical Engineering, Carnegie Mellon University, Pittsburgh, PA 15213, United States.
Jorge L
JL
Jimenez-Rios
Biothermal Technology Laboratory, Department of Mechanical Engineering, Carnegie Mellon University, Pittsburgh, PA 15213, United States. Electronic address: rabin@cmu.edu.
Yoed
Y
Rabin
D000818:Animals; D001808:Blood Vessels / Q000502:physiology*; D002072:Butylene Glycols / Q000378:metabolism; D015925:Cryopreservation / Q000295:instrumentation / Q000379:methods*; D003451:Cryoprotective Agents / Q000378:metabolism*; D003511:Cyclohexanols / Q000378:metabolism; D004867:Equipment Design; D006041:Goats; D007053:Ice / Q000032:analysis; D010539:Permeability; D011092:Polyethylene Glycols / Q000378:metabolism; D013314:Stress, Mechanical; D013696:Temperature; D058989:Vitrification
D002072:Butylene Glycols; D003451:Cryoprotective Agents; D003511:Cyclohexanols; D007053:Ice; D011092:Polyethylene Glycols; C026978:2,3-butylene glycol; C007192:cyclohexane-1,3-diol
Cryopreservation; Solid mechanics; Synthetic ice modulators; Thermal expansion; Thermal stress; Vitrification