Protective effects of Lactobacillus plantarum CCFM8610 against chronic cadmium toxicity in mice indicate routes of protection besides intestinal sequestration.
Our previous study confirmed the ability of Lactobacillus plantarum CCFM8610 to protect against acute cadmium (Cd) toxicity in mice. This study was designed to evaluate the protective effects of CCFM8610 against chronic Cd toxicity in mice and to gain insights into the protection mode of this strain. Experimental mice were divided into two groups and exposed to Cd for 8 weeks via drinking water or intraperitoneal injection. Both groups were further divided into four subgroups, control, Cd only, CCFM8610 only, and Cd plus CCFM8610. Levels of Cd were measured in the feces, liver, and kidneys, and alterations of several biomarkers of Cd toxicity were noted. The results showed that when Cd was introduced orally, cotreatment with Cd and CCFM8610 effectively decreased intestinal Cd absorption, reduced Cd accumulation in tissue, alleviated tissue oxidative stress, reversed hepatic and renal damage, and ameliorated the corresponding histopathological changes. When Cd was introduced intraperitoneally, administration of CCFM8610 did not have an impact on tissue Cd accumulation or reverse the activities of antioxidant enzymes. However, CCFM8610 still offered protection against oxidative stress and reversed the alterations of Cd toxicity biomarkers and tissue histopathology. These results suggest that CCFM8610 is effective against chronic cadmium toxicity in mice. Besides intestinal Cd sequestration, CCFM8610 treatment offers direct protection against Cd-induced oxidative stress. We also provide evidence that the latter is unlikely to be mediated via protection against Cd-induced alteration of antioxidant enzyme activities.
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China.
Qixiao
Q
Zhai
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China.
Gang
G
Wang
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China.
Jianxin
J
Zhao
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China.
Xiaoming
X
Liu
Gut Health and Food Safety Programme, Institute of Food Research, Norwich, United Kingdom.
Arjan
A
Narbad
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China Synergistic Innovation Center for Food Safety and Nutrition, Wuxi, People's Republic of China.
Yong Q
YQ
Chen
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China.
Hao
H
Zhang
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China fwtian@jiangnan.edu.cn.
Fengwei
F
Tian
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China Synergistic Innovation Center for Food Safety and Nutrition, Wuxi, People's Republic of China.
Wei
W
Chen
D000818:Animals; D002104:Cadmium / Q000032:analysis / Q000633:toxicity*; D002105:Cadmium Poisoning / Q000517:prevention & control*; D004195:Disease Models, Animal; D005243:Feces / Q000737:chemistry; D006651:Histocytochemistry; D007668:Kidney / Q000737:chemistry / Q000473:pathology; D048191:Lactiplantibacillus plantarum / Q000254:growth & development* / Q000378:metabolism*; D008099:Liver / Q000737:chemistry / Q000473:pathology; D051379:Mice; D019936:Probiotics / Q000008:administration & dosage*
D002104:Cadmium