Interleukins in gingival crevicular fluid in patients with definitive full-coverage restorations.
The objective of this study was to determine interleukin (IL)-1α and IL-8 levels in the gingival crevicular fluid (GCF) of patients with different levels of crown margin placements. Samples of GCF were obtained from 12 study participants with definitive full-coverage restorations with supragingival or equigingival crown margin placements. The periodontal status of the volunteers ranged from healthy to generalized severe periodontitis. Pocket depth and bleeding on probing were assessed at the clinical examination, and interleukin concentrations were determined by enzyme-linked immunosorbent assay (ELISA). Analysis of variance (ANOVA) was used to statistically compare interleukin concentrations between the control, supragingival, and equigingival GCF samples. Compared to controls (60.4 ± 8.9 pg/mL), the average IL-1α concentration in the GCF samples surrounding the supragingival margins was 53.8 ± 9.7 pg/mL and was 110.5 ± 23.3 pg/mL in the equigingival margins. Compared to controls (59.0 ± 14.1 pg/mL), the average IL-8 concentration in the supragingival margins was 46.9 ± 9.7 pg/mL and was 131.4 ± 27.5 pg/mL in the equigingival margins. The trend of higher levels of interleukins in GCF corresponding to equigingival margins was consistent, as was the trend of lower concentrations in supragingival margins compared to the controls; however, statistical significance was not achieved because of the wide biological variation within and between patients. In conclusion, differences in GCF IL-1α and IL-8 concentrations were observed when comparing fixed crown restorations with equigingival and supragingival margins. Gingival inflammation may be dependent on the periodontal condition in addition to restoration or margin placement.
Adjunct Clinical Faculty, Department of Periodontics, Ostrow School of Dentistry, University of Southern California, Los Angeles, California; Former Graduate Student, Department of Periodontics and Dental Hygiene, School of Dentistry, University of Detroit Mercy, Detroit, Michigan.
Kai-Chiao J
KC
Chang
Associate Professor, Department of Biomedical and Diagnostic Sciences, School of Dentistry, University of Detroit Mercy, Detroit, Michigan.
Michelle A
MA
Wheater
Associate Professor, Department of Periodontics and Dental Hygiene, School of Dentistry, University of Detroit Mercy, Detroit, Michigan.
Levyee
L
Cabanilla Jacobs
Adjunct Assistant Professor, Department of Periodontics and Dental Hygiene, School of Dentistry, University of Detroit Mercy, Detroit, Michigan.
Luis A
LA
Litonjua
D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D003793:Dental Restoration, Permanent; D004797:Enzyme-Linked Immunosorbent Assay; D005883:Gingival Crevicular Fluid / Q000378:metabolism*; D006801:Humans; D007378:Interleukins / Q000378:metabolism*; D008875:Middle Aged
D007378:Interleukins