Use of fluorescence quenching method to measure sorption constants of phenolic xenoestrogens onto humic fractions from sediment.
Humic substance (HS) in sediment can affect hydrophobic organic compound distribution, transportation, bioavailability, and toxicity. This study investigated the HS (BKHS) extracted from sediment and separated it into low molecular humic (LMHS, <1kDa) and high molecular humic substances (HMHS, 1kDa-0.45μm). Nonylphenol (NP), octylphenol (OP), and bisphenol A (BPA) have a significant sorption capacity for HMHS and BKHS solutions. They are xenoestrogenic endocrine-disrupting compounds that are widely produced and discharged to the environment. The log KHS values of the BKHS and HMHS solutions were between 4.74-5.09Lkg-C(-1) and 4.57-5.09Lkg-C(-1), respectively. However, the three compounds were not sorbed by the LMHS solution. The average values of SUVA254 for HMHS and LMHS were 4.29 and 1.31Lm(-1)mg-C(-1) and the average values of A250-400 for HMHS and LMHS were 18.1 and 4.51nmcm(-1), respectively. The HMHS peak position in the fluorescence excitation/emission matrix at longer wavelengths corresponded to the peak position of LMHS, which indicates that the HMHS had a higher degree of humification than the LMHS. The results suggested that the KHS value's dominant factor was the degree of HS humification.
Department of Civil Engineering, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan; Emerging Compounds Research Center, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan.
Yi-Lung
YL
Yeh
Emerging Compounds Research Center, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan; Department of Environmental Science and Engineering, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan.
Kuei-Jyum
KJ
Yeh
Department of Applied English, Tainan University of Technology, Tainan City 71002, Taiwan.
Liang-Fong
LF
Hsu
Department of Environmental Science and Engineering, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan.
Wu-Chou
WC
Yu
Department of Soil and Water Conservation, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan.
Ming-His
MH
Lee
Emerging Compounds Research Center, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan; Department of Environmental Science and Engineering, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan. Electronic address: chen5637@mail.npust.edu.tw.
Ting-Chien
TC
Chen
D065966:Absorption, Physicochemical; D052244:Endocrine Disruptors / Q000737:chemistry*; D004968:Estrogens, Non-Steroidal / Q000737:chemistry*; D019015:Geologic Sediments / Q000737:chemistry*; D006812:Humic Substances / Q000032:analysis*; D008956:Models, Chemical; D010636:Phenols / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D015262:Xenobiotics / Q000737:chemistry*
D052244:Endocrine Disruptors; D004968:Estrogens, Non-Steroidal; D006812:Humic Substances; D010636:Phenols; D015262:Xenobiotics
Excitation–emission matrix (EEM); Fluorescence quenching; Humic substances; Phenolic xenoestrogens; UV/vis indicators