Fibrillation of β amyloid peptides in the presence of phospholipid bilayers and the consequent membrane disruption.
Fibrillation of β amyloid (Aβ) peptides and the accumulation of amyloid plaques are considered as an important clinical hallmark to identify Alzheimer's disease (AD). The physiological connection between Aβ plaques and the disruption of neuronal cells has not been clearly understood. One hypothesis to explain the Aβ neurotoxicity is that the fibrillation process induces disruption to the cellular membrane. We studied the Aβ fibrillation process in two biologically relevant conditions with the peptide either pre-incorporated into or externally added to the synthetic phospholipid bilayers. These two sample preparation conditions mimic the physiological membrane proximities of Aβ peptides before and after the enzymatic cleavage of amyloid precursor protein (APP). Using thioflavin T (ThT) fluorescence and transmission electron microscopy (TEM), we were able to monitor the kinetics and morphological evolution of fibril formation, which was highly sensitive to the two sample preparation protocols. While the external addition protocol generates long and mature fibrils through normal fibrillation process, the pre-incubation protocol was found to stabilize the immature protofibrils. Fluorescence spectroscopy studies with doubly-labeled phospholipids indicated that there may be a lipid uptake process associated with the fibril formation. Solid state nuclear magnetic resonance (NMR) spectroscopy provided evidence for high resolution structural variations in fibrils formed with different protocols, and in particular the stabilization of long-range contact between N- and C-terminal β strands. In addition, disruption of phospholipid bilayers was supported by measurements with ³¹P chemical shifts and relaxation time constants.
Department of Chemistry, Binghamton University, State University of New York, Binghamton, NY 13902-6000, USA; Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892-0520, USA. Electronic address: wqiang@binghamton.edu.
Wei
W
Qiang
Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892-0520, USA.
Wai-Ming
WM
Yau
Department of Chemistry, Binghamton University, State University of New York, Binghamton, NY 13902-6000, USA.
Jürgen
J
Schulte
D016229:Amyloid beta-Peptides / Q000737:chemistry* / Q000633:toxicity; D008051:Lipid Bilayers / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008954:Models, Biological; D010743:Phospholipids / Q000737:chemistry*; D066263:Protein Aggregation, Pathological
D016229:Amyloid beta-Peptides; D008051:Lipid Bilayers; D010743:Phospholipids
Alzheimer's disease; Membrane disruption; Neurotoxicity mechanism; Solid state NMR; β amyloid fibril