Simultaneous imaging of neural activity in three dimensions.
We introduce a scanless optical method to image neuronal activity in three dimensions simultaneously. Using a spatial light modulator and a custom-designed phase mask, we illuminate and collect light simultaneously from different focal planes and perform calcium imaging of neuronal activity in vitro and in vivo. This method, combining structured illumination with volume projection imaging, could be used as a technological platform for brain activity mapping.
Department of Biological Sciences, Columbia University New York, NY, USA.
Sean
S
Quirin
Department of Biological Sciences, Columbia University New York, NY, USA.
Jesse
J
Jackson
Department of Biological Sciences, Columbia University New York, NY, USA.
Darcy S
DS
Peterka
Department of Biological Sciences, Columbia University New York, NY, USA.
Rafael
R
Yuste
D000818:Animals; D006624:Hippocampus / Q000502:physiology; D007091:Image Processing, Computer-Assisted; D021621:Imaging, Three-Dimensional / Q000379:methods*; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000502:physiology*; D015027:Zebrafish
brain activity map; calcium imaging; spatial-light-modulator; three-dimensional imaging; volume imaging