Plasmid-related quinolone resistance determinants in epidemic Vibrio parahaemolyticus, uropathogenic Escherichia coli, and marine bacteria from an aquaculture area in Chile.
Marine bacteria from aquaculture areas with industrial use of quinolones have the potential to pass quinolone resistance genes to animal and human pathogens. The VPA0095 gene, related to the quinolone resistance determinant qnrA, from clinical isolates of epidemic Vibrio parahaemolyticus conferred reduced susceptibility to quinolone after cloning into Escherichia coli K-12 either when acting alone or synergistically with DNA gyrase mutations. In addition, a plasmid-mediated quinolone resistance gene from marine bacteria, aac(6')-Ib-cr, was identical to aac(6')-Ib-cr from urinary tract isolates of E. coli, suggesting a recent flow of this gene between these bacteria isolated from different environments. aac(6')-Ib-cr from E. coli also conferred reduced susceptibility to quinolone and kanamycin when cloned into E. coli K-12.
Department of Microbiology and Immunology, New York Medical College, Basic Science Building, Valhalla, NY, 10595, USA.
Sandra
S
Aedo
Larisa
L
Ivanova
Alexandra
A
Tomova
Felipe C
FC
Cabello
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D017756:Aquaculture; D002677:Chile; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009154:Mutation; D010957:Plasmids / Q000235:genetics; D015363:Quinolones / Q000494:pharmacology*; D056689:Uropathogenic Escherichia coli / Q000187:drug effects* / Q000235:genetics; D014736:Vibrio parahaemolyticus / Q000187:drug effects* / Q000235:genetics
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D015363:Quinolones