Morphofunctional analysis of human platelets by vital staining.
We developed a method for differential staining of human platelets preserving their functional activity based on vital fl uorochrome stains trypafl avin and acridine orange. Platelets stained with trypafl avin and acridine orange exhibited under a fl uorescent microscope green fl uorescence of the cytoplasm and red-orange fl uorescence of the granules. Morphofunctional analysis of platelets was carried out on the cells from donor blood, donor concentrated platelets, and cells from hematological patients and patients with thromboembolic complications. Populations with low (16 %) and high (2 %) morphofunctional activities of platelets were detected among donors. The morphofunctional parameters of platelets were sharply reduced in hematological patients with the hemorrhagic syndrome and elevated signifi cantly in patients with thromboembolic complications in comparison with donors. The method seemed to be effective for evaluating the platelet quality in donor and patients' blood components.
N. V. Sklifosovsky Research Institute of Emergency Medicine, Moscow, Russia, mcsimmc@yandex.ru.
M S
MS
Makarov
E N
EN
Kobzeva
I V
IV
Vysochin
N V
NV
Borovkova
V T
VT
Khvatov
D001792:Blood Platelets / Q000378:metabolism*; D006801:Humans; D013194:Staining and Labeling