Detection of Mycobacterium tuberculosis complex by PCR in fresh cheese from local markets in Hidalgo, Mexico.
The objective of this study was to identify the presence of Mycobacterium tuberculosis complex bacterial DNA in samples extracted from fresh cheeses; 95 samples of fresh cheese were obtained from municipal markets in the state of Hidalgo, in central Mexico, and were analyzed in triplicate. The exogenous control for the amplification was the mitochondrial gene for cytochrome b (cyt-b). M. tuberculosis complex DNA was detected by nested-PCR amplification of a fragment of the mpb70 gene in six samples, four of which were obtained from regions with enzootic bovine tuberculosis. These results suggest that cheeses prepared with raw milk contaminated with M. bovis are being sold and consumed by humans, which may cause tuberculosis.
Department of Physiology, University Center for Health Sciences, University of Guadalajara, Guadalajara, Jalisco 44340, Mexico.
Ana Laura
AL
Pereira-Suárez
Faculty of Graduate Studies Cuautitlan (FESC), National Autonomous University of Mexico (UNAM), Cuautitlán Izcallí, State of Mexico 54740, Mexico.
Yessica
Y
Estrada-Chávez
State Public Health Laboratory of Hidalgo, Hidalgo 42059, Mexico.
Armidá
A
Zúñiga-Estrada
Unit of Medical and Pharmaceutical Biotechnology, Center for Research and Assistance in Technology and Design of the State of Jalisco Civil Association, Guadalajara, Jalisco 44270, Mexico.
Gonzálo
G
Lopez-Rincón
Unit of Medical and Pharmaceutical Biotechnology, Center for Research and Assistance in Technology and Design of the State of Jalisco Civil Association, Guadalajara, Jalisco 44270, Mexico.
David Ulises Miranda
DU
Hernández
Department of Animal Production, University Center for Biological and Agricultural Sciences, Universidad de Guadalajara, Guadalajara, Jalisco 45110, Mexico.
Francisco Javier
FJ
Padilla-Ramírez
Unit of Medical and Pharmaceutical Biotechnology, Center for Research and Assistance in Technology and Design of the State of Jalisco Civil Association, Guadalajara, Jalisco 44270, Mexico. cestrada.ciatej@gmail.com.
Ciro
C
Estrada-Chávez
D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism; D002417:Cattle; D002611:Cheese / Q000191:economics / Q000382:microbiology*; D045303:Cytochromes b / Q000235:genetics; D005506:Food Contamination / Q000032:analysis*; D008800:Mexico; D008892:Milk / Q000382:microbiology*; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D014380:Tuberculosis, Bovine / Q000382:microbiology
D001426:Bacterial Proteins; D045303:Cytochromes b