[Determination of triterpenoic acid isomers in Eriobotryae folium using high performance liquid chromatography on C30 column].
A rapid and easy assay method for four triterpenoic acid isomers in Eriobotryae folium was established. The sample was extracted with methanol, then separated on a C30 column (250 mm x 4.6 mm, 5 microm) at 20 degrees C using acetonitrile-water (95 : 5, v/v) as the mobile phase at a flow rate of 1.0 mL/min. The target compounds were simultaneously detected at 210 nm, and the injection volume was 10 microL. The validation was carried out and the resolution (R > or = 2.2), the precision (RSD < or = 1.1%), the linearity (r > or = 0.999 2), the repeatability (RSD < or = 4.4%), and the recovery (range from 95.4% to 101.7%, RSD < or = 4.8%) were acceptable. The method is reliable for the quantification of the four triterpenoic acid isomers by HPLC on C30 column.
Yong
Y
Zhang
Kunpeng
K
Xue
An
A
Zhou
Huimin
H
Yu
Yin
Y
Li
Lixin
L
Yao
Yuexing
Y
Zhao
Xiaomei
X
Xie
D002851:Chromatography, High Pressure Liquid; D031983:Eriobotrya / Q000737:chemistry*; D007536:Isomerism; D014315:Triterpenes / Q000032:analysis*
D014315:Triterpenes