Immunoreactivity of protein tyrosine phosphatase A (PtpA) in sera from sheep infected with Mycobacterium avium subspecies paratuberculosis.
Evasion of host defense mechanisms and survival inside infected host macrophages are features of pathogenic mycobacteria including Mycobacterium avium subspecies paratuberculosis, the causative agent of Johne's disease in ruminants. Protein tyrosine phosphatase A (PtpA) has been identified as a secreted protein critical for survival of mycobacteria within infected macrophages. The host may mount an immune response to such secreted proteins. In this study, the humoral immune response to purified recombinant M. avium subsp. paratuberculosis PtpA was investigated using sera from a cohort of sheep infected with M. avium subsp. paratuberculosis and compared with uninfected healthy controls. A significantly higher level of reactivity to PtpA was observed in sera collected from M. avium subspecies paratuberculosis infected sheep when compared to those from uninfected healthy controls. PtpA could be a potential candidate antigen for detection of humoral immune responses in sheep infected with M. avium subspecies paratuberculosis.
Faculty of Veterinary Science, University of Sydney, Camden, NSW, Australia.
Ratna B
RB
Gurung
Faculty of Veterinary Science, University of Sydney, Camden, NSW, Australia.
Douglas J
DJ
Begg
Faculty of Veterinary Science, University of Sydney, Camden, NSW, Australia.
Auriol C
AC
Purdie
Department of Medicine, Division of Infectious Diseases, University of British Columbia, Vancouver, BC, Canada.
Horacio
H
Bach
Faculty of Veterinary Science, University of Sydney, Camden, NSW, Australia. Electronic address: richard.whittington@sydney.edu.au.
Richard J
RJ
Whittington
D000818:Animals; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D015971:Gene Expression Regulation, Enzymologic / Q000276:immunology*; D016927:Mycobacterium avium subsp. paratuberculosis; D010283:Paratuberculosis / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology; D017027:Protein Tyrosine Phosphatases / Q000235:genetics / Q000378:metabolism*; D012756:Sheep; D012757:Sheep Diseases / Q000201:enzymology* / Q000276:immunology / Q000382:microbiology
D017027:Protein Tyrosine Phosphatases
ELISA; Lesion; Paratuberculosis; PtpA; Serum; Sheep