Vitamin E improved cypermethrin-induced damages in the ovary of rats; evidence for angiogenesis and p53 involvement.
This study aimed to investigate the protective effect of vitamin E (VitE) on cypermethrin (CPM)-induced damages in the ovary. Wistar rats were divided into seven groups (n=6) including; control-sham (c), CPM-received (CPM, 75 mg/kg, i.p.), and CPM and VitE-treated (VitE, 150 mg/kg, orally) for 7, 14 and 24 days. The antioxidant status determination and hormonal assays along with histological and immunofluorescent assessments were performed. The expression of p53 at mRNA level was also examined. The CPM administration affected the ovarian structure and functions as it elevated the follicular atresia and significantly (P<0.05) lowered the estradiol level, time dependently. VitE administration enhanced the CPM-reduced antioxidant capacity, gonadotropins and estradiol levels. Co-administration of VitE and CPM remarkably attenuated the CPM-induced RNA damage in granulosa and theca cells and elevated the deranged angiogenesis. The CPM-reduced micro and macro vessels distribution was significantly (P<0.05) elevated in the VitE-received animals. Expression of p53 at mRNA level was down regulated in the VitE-treated groups completely and relatively following 7 and 14 days, respectively. Our data showed that the CPM-induced biochemical and histological damages could be prevented by VitE. Moreover, protective effects of VitE attribute to its potency in enhancing the antioxidant capacity and promoting the gonadotropins secretion, which resulted in down regulation of p53 overexpression and RNA damage in follicular cells accomplished with improved angiogenesis.
Department of Pathology, Faculty of Veterinary Medicine, Islamic Azad University, Urmia Branch, Urmia, Iran.
Morteza
M
Molavi
Department of Histology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran. Electronic address: Mazdak.razi@gmail.com.
Mazdak
M
Razi
Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
Hassan
H
Malekinejad
Department of Pathology, Faculty of Veterinary Medicine, Islamic Azad University, Urmia Branch, Urmia, Iran.
Amir
A
Amniattalab
Department of Pathology, Faculty of Veterinary Medicine, Islamic Azad University, Urmia Branch, Urmia, Iran.
Hamed
H
Rezaie
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D005260:Female; D007306:Insecticides / Q000633:toxicity*; D008315:Malondialdehyde / Q000378:metabolism; D010053:Ovary / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D011722:Pyrethrins / Q000633:toxicity*; D012333:RNA, Messenger / Q000378:metabolism; D017208:Rats, Wistar; D016159:Tumor Suppressor Protein p53 / Q000235:genetics; D014810:Vitamin E / Q000494:pharmacology*
D000975:Antioxidants; D007306:Insecticides; D011722:Pyrethrins; D012333:RNA, Messenger; D016159:Tumor Suppressor Protein p53; D014810:Vitamin E; C017160:cypermethrin; D008315:Malondialdehyde
Angiogenesis; Cypermethrin; Ovary; RNA damage; Vitamin E; p53 expression