Superparamagnetic iron oxide magnetic nanomaterial-labeled bone marrow mesenchymal stem cells for rat liver repair after hepatectomy.
BACKGROUND
Superparamagnetic iron oxide magnetic nanomaterials (SPIO) are tracers used for treatment of central nervous and cardiovascular system complications in animal models. The present study investigated survival and proliferation of SPIO-labeled bone marrow mesenchymal stem cells (BMSCs) to determine their potential therapeutic value in liver repair.


METHODS
Surface antigens of BMSCs were measured using flow cytometry. BMSCs viability, growth curve, and SPIO (0-100 μg/mL) labeling rate were evaluated using trypan blue staining, 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), and Prussian blue staining, respectively. SPIO-labeled BMSCs were transplanted via liver or spleen injection in rats undergoing 70% hepatectomy. Distribution of SPIO-labeled BMSCs in liver and spleen, and liver repair were evaluated by magnetic resonance imaging (MRI), and serum alanine aminotransferase, aspartate aminotransferase, albumin, and total bilirubin levels.


RESULTS
CD29(+)/CD90(+)/CD45(-) BMSCs were successfully isolated from rats. Labeling rate of SPIO in 25 μg/mL was 94.9%. SPIO labeling did not affect BMSCs survival and proliferation. MRI showed that BMSCs colonized in the liver, whether via spleen or liver injection. Serum levels of alanine aminotransferase, aspartate aminotransferase, and total bilirubin in the transplanted rats were significantly lower than in the hepatectomy group at days 1, 3, and 7 after hepatectomy (all P < 0.05), whereas serum albumin levels were significantly higher in the transplanted rats on posthepatectomy day 3 (both P < 0.05). These indicators were not significantly different between the spleen and liver injection approaches.


CONCLUSIONS
BMSCs transplantation via liver or spleen injection could significantly accelerate liver healing. In vivo MRI of SPIO-labeled BMSCs can be used to trace real-time liver healing during clinical treatment after hepatectomy.
Department of Hepatobiliary Surgery, The First Affiliated Hospital of Dalian Medical University, Dalian, China.
Shiyu
S
Zhao
Department of Hepatobiliary Surgery, The First Affiliated Hospital of Dalian Medical University, Dalian, China.
Yulin
Y
Wang
Department of Anaesthesia, The Second Affiliated Hospital of Dalian Medical University, Dalian, China.
Chengshun
C
Gao
Department of Hepatobiliary Surgery, The First Affiliated Hospital of Dalian Medical University, Dalian, China.
Jing
J
Zhang
Department of Hepatobiliary Surgery, The First Affiliated Hospital of Dalian Medical University, Dalian, China.
Haidong
H
Bao
Department of Hepatobiliary Surgery, The First Affiliated Hospital of Dalian Medical University, Dalian, China.
Zhongyu
Z
Wang
Department of Hepatobiliary Surgery, The First Affiliated Hospital of Dalian Medical University, Dalian, China. Electronic address: doctorgongpeng@hotmail.com.
Peng
P
Gong
D000818:Animals; D049109:Cell Proliferation; D002470:Cell Survival; D005290:Ferric Compounds; D006498:Hepatectomy; D008115:Liver Regeneration; D008279:Magnetic Resonance Imaging; D058185:Magnetite Nanoparticles; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley
D005290:Ferric Compounds; D058185:Magnetite Nanoparticles; C000499:ferric oxide
Bone marrow mesenchymal stem cells; Hepatectomy; Liver repair; Magnetic resonance imaging; Superparamagnetic iron oxide magnetic nanomaterials