Rabbit MSTN gene polymorphisms and genetic effect analysis.
We analyzed meat samples of nine pure lines of rabbit and its 37 hybrid combinations by sequencing and single-strand conformation polymorphism techniques to explore genetic polymorphisms of all the three exon regions and part of the 5'-regulatory region of the myostatin (MSTN) gene. Thus, we detected a single nucleotide mutation (T→C) on the 476 locus of the 5'-regulatory region, but no mutation sites were detected in the exon areas. The correlation analysis showed that the mutation had some favorable genetic effects, and it resulted in increased liver weight, carcass weight, forelegs weight, back and waist weight, ham weight, and tare weight, whereas it decreased muscle drip loss and cooking loss (P < 0.05). These results suggest that the mutations in the upstream regulatory region of the MSTN gene are beneficial to the rabbit soma development, and the mutations can be used as molecular markers for the selection of the meat quality of rabbits.
College of Animal Science and Technology, Shandong Agricultural University, Tai'an, China.
X B
XB
Qiao
Shandong University of Traditional Chinese Medicine, Jinan, China.
K Y
KY
Xu
College of Animal Science and Technology, Shandong Agricultural University, Tai'an, China.
B
B
Li
College of Animal Science and Technology, Shandong Agricultural University, Tai'an, China.
X
X
Luan
College of Animal Science and Technology, Shandong Agricultural University, Tai'an, China.
T
T
Xia
College of Animal Science and Technology, Shandong Agricultural University, Tai'an, China fanxinzhong_fxz@yeah.net.
X Z
XZ
Fan
D000818:Animals; D006720:Homozygote; D008460:Meat; D024510:Muscle Development / Q000235:genetics*; D009154:Mutation; D055435:Myostatin / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D018807:Polymorphism, Single-Stranded Conformational; D011817:Rabbits; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics
D055435:Myostatin