Species and endosymbiont diversity of Bemisia tabaci (Homoptera: Aleyrodidae) on vegetable crops in Senegal.
Bemisia tabaci-transmitted geminiviruses are one of the major threats on cassava and vegetable crops in Africa. However, to date, few studies are available on the diversity of B. tabaci and their associated endosymbionts in Africa. More than 28 species have been described in the complex of B. tabaci cryptic species; among them, 2 are invasive pests worldwide: MED and MEAM1. In order to assess the species diversity of B. tabaci in vegetable crops in Senegal, several samplings in different localities, hosts and seasons were collected and analyzed with nuclear (microsatellite) and mitochondrial (COI) markers. The bacterial endosymbiont community was also studied for each sample. Two species were detected: MED Q1 and MEAM1 B. Patterns of MED Q1 (dominance on most of the samples and sites, highest nuclear and mitochondrial diversity and broader secondary endosymbiont community: Hamiltonella, Cardinium, Wolbachia and Rickettsia), point toward a predominant resident begomovirus vector group for MED Q1 on market gardening crops. Furthermore, the lower prevalence of the second species MEAM1 B, its lower nuclear and mitochondrial diversity and a narrower secondary endosymbiont community (Hamiltonella/Rickettsia), indicate that this genetic group is exotic and results from a recent invasion in this area.
CIRAD, UMR PVBMT, F-97410 Saint-Pierre, La Réunion, France.
Delatte
D
Hélène
CIRAD, UMR PVBMT, F-97410 Saint-Pierre, La Réunion, France.
Baudin
B
Rémy
Museum National d'Histoire Naturelle, Institut de Systématique, Evolution, Biodiversité, ISYEB, UMR 7205 CNRS, UPMC, EPHE, 75005, Paris, France.
Becker
B
Nathalie
TECHNISEM, Zone d'activité Anjou Actiparc de Jumelles, 49160, Longué-Jumelles, France.
Girard
G
Anne-Laure
TECHNISEM, Zone d'activité Anjou Actiparc de Jumelles, 49160, Longué-Jumelles, France.
Ramatoulaye Sidebe
RS
Traoré
CIRAD, UMR PVBMT, F-97410 Saint-Pierre, La Réunion, France.
Lett
L
Jean-Michel
CIRAD, UMR PVBMT, F-97410 Saint-Pierre, La Réunion, France.
Reynaud
R
Bernard
D000818:Animals; D001419:Bacteria / Q000235:genetics*; D044822:Biodiversity; D018556:Crops, Agricultural; D004269:DNA, Bacterial / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics; D005260:Female; D006430:Hemiptera / Q000235:genetics / Q000382:microbiology*; D018895:Microsatellite Repeats; D012675:Senegal; D013559:Symbiosis; D014675:Vegetables / Q000469:parasitology
D004269:DNA, Bacterial; D004272:DNA, Mitochondrial
MED and MEAM1 species; begomovirus vectors; endosymbionts diversity; microsatellites; mtCOI phylogeny; whitefly