Evaluation of optimal conditions for determination of low selenium content in shellfish samples collected at Todos os Santos Bay, Bahia, Brazil using HG-AFS.
This work proposes a procedure for the determination of total selenium content in shellfish after digestion of samples in block using cold finger system and detection using atomic fluorescent spectrometry coupled hydride generation (HG AFS). The optimal conditions for HG such as effect and volume of prereduction KBr 10 % (m/v) (1.0 and 2.0 ml) and concentration of hydrochloric acid (3.0 and 6.0 mol L(-1)) were evaluated. The best results were obtained using 3 mL of HCl (6 mol L(-1)) and 1 mL of KBr 10 % (m/v), followed by 30 min of prereduction for the volume of 1 mL of the digested sample. The precision and accuracy were assessed by the analysis of the Certified Reference Material NIST 1566b. Under the optimized conditions, the detection and quantification limits were 6.06 and 21.21 μg kg(-1), respectively. The developed method was applied to samples of shellfish (oysters, clams, and mussels) collected at Todos os Santos Bay, Bahia, Brazil. Selenium concentrations ranged from 0.23 ± 0.02 to 3.70 ± 0.27 mg kg(-1) for Mytella guyanensis and Anomalocardia brasiliana, respectively. The developed method proved to be accurate, precise, cheap, fast, and could be used for monitoring Se in shellfish samples.
Universidade do Estado da Bahia, Rua Silveira Martins, 2555, Cabula, Salvador, Bahia, Brazil, 41195-001, wlopes@uneb.br.
Walter Nei
WN
Lopes Dos Santos
Samuel Marques
SM
Macedo
Sofia Negreiros
SN
Teixeira da Rocha
Caio Niela
CN
Souza de Jesus
Dannuza Dias
DD
Cavalcante
Vanessa
V
Hatje
D000818:Animals; D061006:Bays / Q000737:chemistry; D049872:Bivalvia / Q000737:chemistry / Q000378:metabolism; D001938:Brazil; D004784:Environmental Monitoring / Q000379:methods*; D012643:Selenium / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism; D012758:Shellfish / Q000032:analysis*; D013054:Spectrophotometry, Atomic / Q000379:methods; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*
D014874:Water Pollutants, Chemical; D012643:Selenium