miR-34a expands myeloid-derived suppressor cells via apoptosis inhibition.
Myeloid-derived suppressor cells (MDSCs) are a heterogeneous population and show significant expansion under pathological conditions. microRNA plays important roles in many biological processes, whether microRNAs have a function in the expansion of MDSCs is still not very clear. In this study, miR-34a overexpression can induce the expansion of MDSCs in bone marrow chimera and transgenic mice model. The experimental results suggest that miR-34a inhibited the apoptosis of MDSCs but did not affect the proliferation of MDSCs. The distinct mRNA microarray profiles of MDSCs of wild type and miR-34a over-expressing MDSCs combined with the target prediction of miR-34a suggest that miR-34a may target genes such as p2rx7, Tia1, and plekhf1 to inhibit the apoptosis of MDSCs. Taken together, miR-34a contributes to the expansion of MDSCs by inhibiting the apoptosis of MDSCs.
Institutes of Biology and Medical Sciences, Soochow University, Suzhou 215123, Jiangsu Province, People׳s Republic of China. Electronic address: huang_anfei@163.com.
Anfei
A
Huang
Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou 215021, Jiangsu Province, People׳s Republic of China. Electronic address: zhanghtjp@126.com.
Haitao
H
Zhang
Institutes of Biology and Medical Sciences, Soochow University, Suzhou 215123, Jiangsu Province, People׳s Republic of China. Electronic address: chensisdyxb@126.com.
Si
S
Chen
Institutes of Biology and Medical Sciences, Soochow University, Suzhou 215123, Jiangsu Province, People׳s Republic of China. Electronic address: xiafei87@gmail.com.
Fei
F
Xia
Institutes of Biology and Medical Sciences, Soochow University, Suzhou 215123, Jiangsu Province, People׳s Republic of China. Electronic address: 602744364@qq.com.
Yi
Y
Yang
Institutes of Biology and Medical Sciences, Soochow University, Suzhou 215123, Jiangsu Province, People׳s Republic of China. Electronic address: adiok0903@126.com.
Fulu
F
Dong
Institutes of Biology and Medical Sciences, Soochow University, Suzhou 215123, Jiangsu Province, People׳s Republic of China. Electronic address: dongfl@suda.edu.cn.
Di
D
Sun
Institutes of Biology and Medical Sciences, Soochow University, Suzhou 215123, Jiangsu Province, People׳s Republic of China. Electronic address: sdxiong@suda.edu.cn.
Sidong
S
Xiong
Institutes of Biology and Medical Sciences, Soochow University, Suzhou 215123, Jiangsu Province, People׳s Republic of China. Electronic address: j_pzhang@suda.edu.cn.
Jinping
J
Zhang
D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D051017:Apoptosis Regulatory Proteins / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D023461:Myeloid Progenitor Cells / Q000166:cytology* / Q000378:metabolism*; D038042:Myelopoiesis / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D016601:RNA-Binding Proteins / Q000235:genetics; D058486:Receptors, Purinergic P2X7 / Q000235:genetics; D013154:Spleen / Q000166:cytology / Q000378:metabolism; D000074019:T-Cell Intracellular Antigen-1; D013950:Thymus Gland / Q000166:cytology / Q000378:metabolism; D015854:Up-Regulation
D051017:Apoptosis Regulatory Proteins; C551848:MIRN34a microRNA, mouse; D035683:MicroRNAs; C546469:P2rx7 protein, mouse; D016601:RNA-Binding Proteins; D058486:Receptors, Purinergic P2X7; D000074019:T-Cell Intracellular Antigen-1; C499367:Tia1 protein, mouse
Apoptosis; Expansion; Myeloid-derived suppressor cells; miR-34a