Down-regulation of asparagine synthetase induces cell cycle arrest and inhibits cell proliferation of breast cancer.
Asparagine synthetase (ASNS) is deemed to be a promising therapeutic target for the treatment of several cancers, but its functional role in human breast cancer is still unknown. In this study, we employed RNA interference as an efficient tool to silence endogenous ASNS expression in breast cancer cell lines. The relationship between ASNS expression and breast cancer cell growth was investigated, and the therapeutic value of ASNS in breast cancer was further evaluated. Depletion of ASNS remarkably inhibited the proliferation and colony formation capacity of breast cancer cells and arrested cell cycle in the S phase. Our findings suggest that ASNS may contribute to breast cancer tumorigenesis and could be a potential therapeutic target in human breast cancer.
Department of Breast Surgery, Zhejiang Cancer Hospital, 38 Guangji Road, Hangzhou, 310022, China.
Hongjian
H
Yang
Xiangming
X
He
Yabing
Y
Zheng
Weiliang
W
Feng
Xianghou
X
Xia
Xingfei
X
Yu
Zhiqiang
Z
Lin
D001217:Aspartate-Ammonia Ligase / Q000235:genetics*; D001483:Base Sequence; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology*; D059447:Cell Cycle Checkpoints / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D057809:HEK293 Cells; D006801:Humans; D008969:Molecular Sequence Data; D034622:RNA Interference; D034741:RNA, Small Interfering
D034741:RNA, Small Interfering; D001217:Aspartate-Ammonia Ligase
RNA interference; asparagine synthetase; breast cancer; cell proliferation