Production of allitol from D-psicose by a novel isolated strain of Klebsiella oxytoca G4A4.
A novel bacterium capable of producing allitol from D-psicose was isolated from soil and identified as Klebsiella oxytoca G4A4. An efficient method for the transformation of D-psicose to allitol was achieved through the resting cell reaction. Ribitol as an inducer is suitable for cell cultivation, and cells are most active in Tris-HCl buffer (pH 8.0) at 37 °C with a density of 40 (OD600 nm ). After the reaction, the final conversion rates of the washed cells were approximately 87, 83, and 55% at D-psicose concentrations of 0.25, 0.5, and 1%, respectively. The product from D-psicose was purified and determined to be allitol by high-performance liquid chromatography and nuclear magnetic resonance spectroscopy.
National Engineering Laboratory for Industrial Enzymes, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, P. R., China.
Wenjia
W
Han
Yueming
Y
Zhu
Yan
Y
Men
Jiangang
J
Yang
Can
C
Liu
Yuanxia
Y
Sun
D002851:Chromatography, High Pressure Liquid; D005632:Fructose / Q000378:metabolism*; D041121:Klebsiella oxytoca / Q000302:isolation & purification / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D012988:Soil Microbiology; D013402:Sugar Alcohols / Q000378:metabolism*
D013402:Sugar Alcohols; C003243:psicose; D005632:Fructose; C527823:allitol
Allitol; Biotransformation; D-psicose; Klebsiella oxytoca