Influence of nutrient composition and plant growth regulators on callus induction and plant regeneration in glutinous rice (Oryza sativa L.).
The potential for callus induction and regeneration depends on nutrient composition and plant growth regulators. The aim of the present study was to investigate the effect of nutrient composition and plant growth regulators on callus induction and plant regeneration in the glutinous rice cultivar Khunvang. The effect of 2,4-D concentrations (1, 2, 3, 4 and 5 mg L(-1)) on callus induction and growth were investigated. The results revealed that the highest percentage of callus induction (97%) was observed in MS medium supplemented with 5 mg L(-1) 2,4-D under 16 h Photoperiod. The effects of casein hydrolysate concentrations of casein hydrolysate (0, 300, 500, 700 and 900 mg L(-1)) and proline (0, 300, 500, 700 and 900 mg L(-1)) on callus induction and growth of Khunvang were also observed. The results indicated that the increasing casein hydrolysate and proline concentrations did not show a significant effect on callus growth. However, proline concentration of 900 mg L(-1) yielded 85.67% of callus growth.
K
K
Duangsee
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S
Bunnag
D015084:2,4-Dichlorophenoxyacetic Acid / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D012275:Oryza / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D010937:Plant Growth Regulators / Q000494:pharmacology*; D012038:Regeneration / Q000187:drug effects*; D012639:Seeds / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000502:physiology
D010937:Plant Growth Regulators; D015084:2,4-Dichlorophenoxyacetic Acid