Synthesis of dye conjugates to visualize the cancer cells using fluorescence microscopy.
The clinical diagnosis of most cancers is based on evaluation of histology microscopic slides to view the size and shape of cellular nuclei and morphological structure of tissue. To achieve this goal for in vivo and in-deep tissues, near infrared dyes-bovine serum albumin and immunoglobulin G conjugates were synthesized. The spectral study shows that the absorption and fluorescence of the dye conjugates are in the "tissue optical window" spectral ranges between 650 and 900 nm. The internalization and pinocytosis of the synthesized compounds were investigated at cell level using fluorescence microscopy to obtain the optimal concentration and staining time.
Yang
Y
Pu
Rui
R
Tang
Jianpeng
J
Xue
W B
WB
Wang
Baogang
B
Xu
S
S
Achilefu
D003287:Contrast Media / Q000138:chemical synthesis; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D006801:Humans; D007089:Image Enhancement / Q000379:methods; D007074:Immunoglobulin G / Q000276:immunology*; D061986:MCF-7 Cells; D008856:Microscopy, Fluorescence / Q000379:methods*; D025202:Molecular Diagnostic Techniques / Q000379:methods; D009374:Neoplasms, Experimental / Q000378:metabolism* / Q000473:pathology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000276:immunology*; D013194:Staining and Labeling / Q000379:methods; D013347:Subcellular Fractions / Q000378:metabolism / Q000473:pathology
D003287:Contrast Media; D005456:Fluorescent Dyes; D007074:Immunoglobulin G; D012710:Serum Albumin, Bovine