Development and characterization of a new 12-plex ChrX miniSTR system.
Short tandem repeat (STR) markers are extensively used for human identification as well as paternity and forensic casework. X-chromosome STR (X-STR) markers are a powerful complementary system especially in deficiency paternity testing. This study presents the development and characterization of a new X-chromosomal short tandem repeat (STR) multiplex using short amplicon (<200 bp). A total of 366 samples from Punjabi population and 346 samples from Sindhi population were typed for 11 X-chromosomal STR markers: DXS101, DXS6789, DXS6793, DXS7132, DXS7423, DXS7424, DXS8378, DXS9902, GATA31E08, GATA172D05, and HPRTB along with sex-typing locus, amelogenin. Each marker showed a high degree of polymorphism, and the multiplex was sensitive down to 250 pg of human DNA. A total of 78 alleles were found with 5-11 alleles for each marker. The population data can be used as reference database for Sindhi and Punjabi populations.
National Centre of Excellence in Molecular Biology, University of the Punjab, 87-West Canal Bank Road, Lahore, -53700, Pakistan, israr@cemb.edu.pk.
Muhammad
M
Israr
Ahmad Ali
AA
Shahid
Ziaur
Z
Rahman
Mian Sahib
MS
Zar
Muhammad Saqib
MS
Shahzad
Tayyab
T
Husnain
Celine
C
Pfeifer
Peter
P
Wiegand
D041321:Chromosomes, Human, X; D053836:DNA Degradation, Necrotic; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers; D005006:Ethnicity / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D005819:Genetic Markers; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats; D060885:Multiplex Polymerase Chain Reaction; D010154:Pakistan
D017931:DNA Primers; D005819:Genetic Markers