Identification of a STOP1-like protein in Eucalyptus that regulates transcription of Al tolerance genes.
Tolerance to soil acidity is an important trait for eucalyptus clones that are introduced to commercial forestry plantations in pacific Asian countries, where acidic soil is dominant in many locations. A conserved transcription factor regulating aluminum (Al) and proton (H⁺) tolerance in land-plant species, STOP1 (SENSITIVE TOPROTON RHIZOTOXICITY 1)-like protein, was isolated by polymerase chain reaction-based cloning, and then suppressed by RNA interference in hairy roots produced by Agrobacterium rhizogenes-mediated transformation. Eucalyptus STOP1-like protein complemented proton tolerance in an Arabidopsis thaliana stop1-mutant, and localized to the nucleus in a transient assay of a green fluorescent protein fusion protein expressed in tobacco leaves by Agrobacterium tumefaciens-mediated transformation. Genes encoding a citrate transporting MULTIDRUGS AND TOXIC COMPOUND EXTRUSION protein and an orthologue of ALUMINUM SENSITIVE 3 were suppressed in transgenic hairy roots in which the STOP1 orthologue was knocked down. In summary, we identified a series of genes for Al-tolerance in eucalyptus, including a gene for STOP1-like protein and the Al-tolerance genes it regulates. These genes may be useful for molecular breeding and genomic selection of elite clones to introduce into acid soil regions.
Forestry Research Institute, Oji Paper Co., Ltd., 24-9 Nobono-Cho, Kameyama, Mie 519-0212, Japan.
Yoshiharu
Y
Sawaki
Forestry Research Institute, Oji Paper Co., Ltd., 24-9 Nobono-Cho, Kameyama, Mie 519-0212, Japan; Laboratory of Plant Cell Technology, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
Yuriko
Y
Kobayashi
Forest Technology Laboratories, Research & Development Division, Oji Paper Co., Ltd., 1-10-6 Shinonome, Koto-ku, Tokyo 135-8558, Japan.
Tomonori
T
Kihara-Doi
Forest Technology Laboratories, Research & Development Division, Oji Paper Co., Ltd., 1-10-6 Shinonome, Koto-ku, Tokyo 135-8558, Japan.
Nobuyuki
N
Nishikubo
Forest Technology Laboratories, Research & Development Division, Oji Paper Co., Ltd., 1-10-6 Shinonome, Koto-ku, Tokyo 135-8558, Japan.
Tetsu
T
Kawazu
BioResources Center, RIKEN, 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan.
Masatomo
M
Kobayashi
Laboratory of Plant Cell Technology, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
Yasufumi
Y
Kobayashi
BioResources Center, RIKEN, 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan.
Satoshi
S
Iuchi
Laboratory of Plant Cell Technology, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
Hiroyuki
H
Koyama
Forest Technology Laboratories, Research & Development Division, Oji Paper Co., Ltd., 1-10-6 Shinonome, Koto-ku, Tokyo 135-8558, Japan. Electronic address: sato5424couperin@oji-gr.com.
Shigeru
S
Sato
D000222:Adaptation, Physiological / Q000187:drug effects / Q000235:genetics*; D060054:Agrobacterium / Q000378:metabolism; D000535:Aluminum / Q000633:toxicity*; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D002951:Citrates / Q000378:metabolism; D005052:Eucalyptus / Q000187:drug effects / Q000235:genetics* / Q000502:physiology*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005816:Genetic Complementation Test; D005822:Genetic Vectors / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development; D030821:Plants, Genetically Modified; D034622:RNA Interference / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic / Q000187:drug effects*
D002951:Citrates; D010940:Plant Proteins; D012333:RNA, Messenger; D049452:Green Fluorescent Proteins; D000535:Aluminum
Acid soil; Al tolerance; Eucalyptus; STOP1