A high isoflavone diet decreases 5' adenosine monophosphate-activated protein kinase activation and does not correct selenium-induced elevations in fasting blood glucose in mice.
Selenium (Se) has been implicated as a micronutrient that decreases adenosine monophosphate-activated protein kinase (AMPK) signaling and may increase diabetes risk by reducing insulin sensitivity. Soy isoflavones (IF) are estrogen-like compounds that have been shown to attenuate insulin resistance, hyperglycemia, adiposity, and increased AMPK activation. We hypothesized that a high IF (HIF) diet would prevent the poor metabolic profile associated with high Se intake. The purpose of this study was to examine changes in basal glucose metabolism and AMPK signaling in response to an HIF diet and/or supplemental Se in a mouse model. Male FVB mice were divided into groups receiving either a control diet with minimal IF (low IF) or an HIF diet. Each dietary group was further subdivided into groups receiving either water or Se at a dose of 3 mg Se/kg body weight daily, as Se-methylselenocysteine (SMSC). After 5 months, mice receiving SMSC had elevated fasting glucose (P < .05) and a tendency for glucose intolerance (P = .08). The increase in dietary IF did not result in improved fasting blood glucose. Interestingly, after 6 months, HIF-fed mice had decreased basal AMPK activation in liver and skeletal muscle tissue (P < .05). Basal glucose metabolism was changed by SMSC supplementation as evidenced by increased fasting blood glucose and glucose intolerance. High dietary IF levels did not protect against aberrant blood glucose. In FVB mice, decreased basal AMPK activation is not the mechanism through which Se exerts its effect. These results suggest that more research must be done to elucidate the role of Se and IF in glucose metabolism.
Department of Nutrition, Dietetics, and Food Science, Brigham Young University, Provo, UT, USA.
Michael T
MT
Stallings
Department of Nutrition, Dietetics, and Food Science, Brigham Young University, Provo, UT, USA.
Brandon R
BR
Cardon
Department of Nutrition, Dietetics, and Food Science, Brigham Young University, Provo, UT, USA.
Jeremy M
JM
Hardman
Department of Nutrition, Dietetics, and Food Science, Brigham Young University, Provo, UT, USA.
Tyler A
TA
Bliss
Department of Nutrition, Dietetics, and Food Science, Brigham Young University, Provo, UT, USA.
Scott E
SE
Brunson
Department of Nutrition, Dietetics, and Food Science, Brigham Young University, Provo, UT, USA.
Chris M
CM
Hart
Department of Nutrition, Dietetics, and Food Science, Brigham Young University, Provo, UT, USA.
Maria D
MD
Swiss
Department of Nutrition, Dietetics, and Food Science, Brigham Young University, Provo, UT, USA.
Squire D
SD
Hepworth
Department of Nutrition, Dietetics, and Food Science, Brigham Young University, Provo, UT, USA.
Merrill J
MJ
Christensen
Department of Nutrition, Dietetics, and Food Science, Brigham Young University, Provo, UT, USA.
Chad R
CR
Hancock
D055372:AMP-Activated Protein Kinases / Q000378:metabolism*; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D004032:Diet; D019587:Dietary Supplements; D005215:Fasting; D018149:Glucose Intolerance / Q000209:etiology*; D007333:Insulin Resistance; D007529:Isoflavones / Q000494:pharmacology* / Q000627:therapeutic use; D008099:Liver / Q000187:drug effects; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D018482:Muscle, Skeletal / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D012643:Selenium / Q000009:adverse effects* / Q000494:pharmacology; D017279:Selenocysteine / Q000009:adverse effects / Q000031:analogs & derivatives* / Q000494:pharmacology
D001786:Blood Glucose; D007529:Isoflavones; D010936:Plant Extracts; D017279:Selenocysteine; D055372:AMP-Activated Protein Kinases; D012643:Selenium; C002979:selenomethylselenocysteine
AMPK; FVB mice; Insulin resistance; Isoflavones; LKB1; Metformin; Selenium