Alleviation of plasma homocysteine level by phytoestrogen α-zearalanol might be related to the reduction of cystathionine β-synthase nitration.
Hyperhomocysteinemia is strongly associated with cardiovascular diseases. Previous studies have shown that phytoestrogen α-zearalanol can protect cardiovascular system from hyperhomocysteinemia and ameliorate the level of plasma total homocysteine; however, the underlying mechanisms remain to be clarified. The aim of this research is to investigate the possible molecular mechanisms involved in ameliorating the level of plasma homocysteine by α-zearalanol. By the successfully established diet-induced hyperhomocysteinemia rat models, we found that, after α-zearalanol treatment, the activity of cystathionine β-synthase, the key enzyme in homocysteine metabolism, was significantly elevated and level of nitrative stress in liver was significantly reduced. In correlation with this, results also showed a decreased nitration level of cystathionine β-synthase in liver. Together data implied that alleviation of plasma homocysteine level by phytoestrogen α-zearalanol might be related to the reduction of cystathionine β-synthase nitration.
Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Capital Medical University, No. 10 Xitoutiao, You An Men Wai, Beijing 100069, China ; Beijing Key Laboratory of Metabolic Disorders Related Cardiovascular Diseases, No. 10 Xitoutiao, You An Men Wai, Beijing 100069, China.
Hui
H
Zhang
Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Capital Medical University, No. 10 Xitoutiao, You An Men Wai, Beijing 100069, China ; Beijing Key Laboratory of Metabolic Disorders Related Cardiovascular Diseases, No. 10 Xitoutiao, You An Men Wai, Beijing 100069, China.
Qi
Q
Sun
Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Capital Medical University, No. 10 Xitoutiao, You An Men Wai, Beijing 100069, China ; Beijing Key Laboratory of Metabolic Disorders Related Cardiovascular Diseases, No. 10 Xitoutiao, You An Men Wai, Beijing 100069, China.
Teng
T
Liu
Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Capital Medical University, No. 10 Xitoutiao, You An Men Wai, Beijing 100069, China ; Beijing Key Laboratory of Metabolic Disorders Related Cardiovascular Diseases, No. 10 Xitoutiao, You An Men Wai, Beijing 100069, China.
Lu
L
Ma
Department of Pathology, Beijing Luhe Hospital, Capital Medical University, 82 Xinhuanan Road, Tongzhou District, Beijing 101149, China.
Panpan
P
Zhen
Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Capital Medical University, No. 10 Xitoutiao, You An Men Wai, Beijing 100069, China ; Beijing Key Laboratory of Metabolic Disorders Related Cardiovascular Diseases, No. 10 Xitoutiao, You An Men Wai, Beijing 100069, China.
Ke
K
Wang
Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Capital Medical University, No. 10 Xitoutiao, You An Men Wai, Beijing 100069, China ; Beijing Key Laboratory of Metabolic Disorders Related Cardiovascular Diseases, No. 10 Xitoutiao, You An Men Wai, Beijing 100069, China.
Lingqiao
L
Lu
Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Capital Medical University, No. 10 Xitoutiao, You An Men Wai, Beijing 100069, China ; Beijing Key Laboratory of Metabolic Disorders Related Cardiovascular Diseases, No. 10 Xitoutiao, You An Men Wai, Beijing 100069, China.
Xin
X
Liu
Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Capital Medical University, No. 10 Xitoutiao, You An Men Wai, Beijing 100069, China ; Beijing Key Laboratory of Metabolic Disorders Related Cardiovascular Diseases, No. 10 Xitoutiao, You An Men Wai, Beijing 100069, China.
Xin
X
Zhang
Beijing Stomatological Hospital, Capital Medical University, 4 Tiantanxili, Dongcheng District, Beijing 100050, China.
Dandan
D
Song
Beijing Stomatological Hospital, Capital Medical University, 4 Tiantanxili, Dongcheng District, Beijing 100050, China.
Xiaoyun
X
Zuo
Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Capital Medical University, No. 10 Xitoutiao, You An Men Wai, Beijing 100069, China ; Beijing Key Laboratory of Metabolic Disorders Related Cardiovascular Diseases, No. 10 Xitoutiao, You An Men Wai, Beijing 100069, China.
Huirong
H
Liu
Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Capital Medical University, No. 10 Xitoutiao, You An Men Wai, Beijing 100069, China ; Beijing Key Laboratory of Metabolic Disorders Related Cardiovascular Diseases, No. 10 Xitoutiao, You An Men Wai, Beijing 100069, China.
Wen
0000-0003-4120-0773
W
Wang
D000818:Animals; D003541:Cystathionine beta-Synthase / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation / Q000187:drug effects; D004789:Enzyme Activation / Q000187:drug effects; D005260:Female; D006710:Homocysteine / Q000097:blood*; D020138:Hyperhomocysteinemia / Q000188:drug therapy* / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D009566:Nitrates / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D048789:Phytoestrogens / Q000008:administration & dosage; D051381:Rats; D017208:Rats, Wistar; D016896:Treatment Outcome; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000378:metabolism; D015029:Zeranol / Q000008:administration & dosage / Q000031:analogs & derivatives*
D009566:Nitrates; D048789:Phytoestrogens; C029659:zearalenol; D006710:Homocysteine; C002744:3-nitrotyrosine; D014443:Tyrosine; D015029:Zeranol; D003541:Cystathionine beta-Synthase