[Effects of paeoniflorin on cerebral blood flow and the balance of PGI2/TXA2 of rats with focal cerebral ischemia-reperfusion injury].
This study is to investigate the effects of paeoniflorin on cerebral blood flow and the balance of PGI2/TXA2 of rats with focal cerebral ischemia-reperfusion injury. A total of 72 SD rats (3) were randomly divided into 6 groups: sham operation group, cerebral ischemia-reperfusion model group (I/R gourp), low (10 mg.kg-1), middle (20 mg.kg-1) and high (40 mg.kg-1) doses of paeoniflorin groups and nimrnodipine group. Focal cerebral ischemia in rats was made by inserting a monofilament suture into internal carotid artery for 90 min and then reperfused for 24 h. The effects of paeoniflorin on neurological deficit scores and the infarction volume of brain were detected. Relative regional cerebral blood flow (rCBF) was continuously monitored over ischemic hemispheres by laser-Doppler flowmetry (LDF). The expression of COX-2 in hippocampal CAl region was estimated by immunohistochemistry and the contents of prostacyclin I2 (PGI2), thromboxane A2 (TXA2), and ratio of PGIJ2/TXA2 in serum were measured by ELISA kits. Paeoniflorin significantly ameliorated neurological scores, reduced the infarction volume, and increased regional cerebral blood flow relative to the I/R group. In addition, paeoniflorin could inhibit COX-2 expression and the release of TXA2 and prevent the downregulation of PGI2 induced by I/R injury. The neuroprotective effects of paeoniflorin against focal cerebral ischemia-reperfusion rats might be attributed to improve the supply of injured hemisphere blood flow and adjust the balance between PGI2/TXA2.
Meng-Lin
ML
Rao
Mi
M
Tang
Jin-Yue
JY
He
Zhi
Z
Dong
D015121:6-Ketoprostaglandin F1 alpha / Q000097:blood*; D000818:Animals; D001921:Brain / Q000098:blood supply; D056547:CA1 Region, Hippocampal / Q000378:metabolism; D051546:Cyclooxygenase 2 / Q000378:metabolism; D005960:Glucosides / Q000302:isolation & purification / Q000494:pharmacology*; D020244:Infarction, Middle Cerebral Artery / Q000097:blood / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D008297:Male; D039821:Monoterpenes / Q000302:isolation & purification / Q000494:pharmacology*; D018696:Neuroprotective Agents / Q000302:isolation & purification / Q000494:pharmacology; D029596:Paeonia / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012039:Regional Blood Flow / Q000187:drug effects*; D015427:Reperfusion Injury / Q000378:metabolism / Q000503:physiopathology; D013929:Thromboxane B2 / Q000097:blood*; D006027:Glycosides
D015121:6-Ketoprostaglandin F1 alpha; D051546:Cyclooxygenase 2; D005960:Glucosides; D039821:Monoterpenes; D018696:Neuroprotective Agents; D013929:Thromboxane B2; C015423:peoniflorin; D006027:Glycosides; C489691:Ptgs2 protein, rat