Rapid and sensitive suspension array for multiplex detection of organophosphorus pesticides and carbamate pesticides based on silica-hydrogel hybrid microbeads.
A technique for multiplex detection of organophosphorus pesticides and carbamate pesticides has been developed using a suspension array based on silica-hydrogel hybrid microbeads (SHHMs). The main advantage of SHHMs, which consist of both silica and hydrogel materials, is that they not only could be distinguished by their characteristic reflection peak originating from the stop-band of the photonic crystal but also have low non-specific adsorption of proteins. Using fluorescent immunoassay, the LODs for fenitrothion, chlorpyrifos-methyl, fenthion, carbaryl and metolcarb were measured to be 0.02ng/mL, 0.012ng/mL, 0.04ng/mL, 0.05ng/mL and 0.1ng/mL, respectively, all of which are much lower than the maximum residue limits, as reported in the European Union pesticides database. All the determination coefficients for these five pesticides were greater than 0.99, demonstrating excellent correlations. The suspension array was specific and had no significant cross-reactivity with other chemicals. The results for the detection of pesticide residues collected from agricultural samples using this method agree well with those from liquid chromatography-tandem mass spectrometry. Our results showed that this simple method is suitable for simultaneous detection of these five pesticides residues in fruits and vegetables.
Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing, Jiangsu, China.
Xuan
X
Wang
State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, Jiangsu, China.
Zhongde
Z
Mu
State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, Jiangsu, China.
Fengqi
F
Shangguan
Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing, Jiangsu, China.
Ran
R
Liu
Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing, Jiangsu, China.
Yuepu
Y
Pu
Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing, Jiangsu, China. Electronic address: lhyin@seu.edu.cn.
Lihong
L
Yin
D002219:Carbamates / Q000032:analysis* / Q000737:chemistry; D005638:Fruit / Q000737:chemistry; D020100:Hydrogels / Q000737:chemistry*; D008863:Microspheres; D009943:Organophosphorus Compounds / Q000032:analysis* / Q000737:chemistry; D010575:Pesticides / Q000032:analysis* / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry*; D014675:Vegetables / Q000737:chemistry
D002219:Carbamates; D020100:Hydrogels; D009943:Organophosphorus Compounds; D010575:Pesticides; D012822:Silicon Dioxide
Fluorescent immunoassay; Pesticides; Photonic; Suspension array