[Discrimination of alleles in HLA-C*04:01:01G groups].
The aim of this study was to investigate the relatively frequencies of alleles in the HLA-C*04:01:01G group and to analyze their relations with HLA-A and -B loci. DNA samples previously typed as HLA-C*04:01:01G were sequentially selected. The sequences for exon 2 to 7 of the HLA-C locus were analyzed by polymerase chain reaction sequence-based typing(PCR-SBT). The HLA-A, -B, -DRB1 and -DQB1 loci were genotyped using PCR-SBT method. The results showed that 178 samples (94.2%) and 11 samples (5.8%) were assigned as HLA-C*04:01:01 and HLA-C*04:82 respectively among 189 samples previously typed as HLA-C*04:01:01G. 72 haplotypes associated with HLA-C*04:01:01 and C*04:82 were found, in which the frequencies of 26 haplotypes were over 0.0050. HLA-C*04:01:01 was strongly related with A*02:03, A*02:07, A*11:01, A*33:03, B*13:01, B*15:01, B*15:05, B*15:27, B*40:01, B*54:01 alleles, while HLA-C*04:82 was related with B*40:01. It is concluded that HLA-C*04:01:01 and HLA-C*04:82 alleles were confirmed in the HLA-C*04:01:01G group, which should be discriminated by the routine HLA genotyping.
Blood Center of Zhejiang Province, Key Laboratory of Blood Safety Research of Ministry of Health, Hangzhou 310006, Zhejiang Province, China.
Wei
W
Wang
Blood Center of Zhejiang Province, Key Laboratory of Blood Safety Research of Ministry of Health, Hangzhou 310006, Zhejiang Province, China.
Nan-Yin
NY
Chen
Blood Center of Zhejiang Province, Key Laboratory of Blood Safety Research of Ministry of Health, Hangzhou 310006, Zhejiang Province, China.
Wei
W
Zhang
Blood Center of Zhejiang Province, Key Laboratory of Blood Safety Research of Ministry of Health, Hangzhou 310006, Zhejiang Province, China.
Jun-Jun
JJ
He
Blood Center of Zhejiang Province, Key Laboratory of Blood Safety Research of Ministry of Health, Hangzhou 310006, Zhejiang Province, China.
Zhe-Dong
ZD
Hang
Blood Center of Zhejiang Province, Key Laboratory of Blood Safety Research of Ministry of Health, Hangzhou 310006, Zhejiang Province, China.
Fei
F
Qin
Blood Center of Zhejiang Province, Key Laboratory of Blood Safety Research of Ministry of Health, Hangzhou 310006, Zhejiang Province, China.
Li-Na
LN
Dong
Blood Center of Zhejiang Province, Key Laboratory of Blood Safety Research of Ministry of Health, Hangzhou 310006, Zhejiang Province, China. E-mail: zfm00@hotmail.com.
Fa-Ming
FM
Zhu
Blood Center of Zhejiang Province, Key Laboratory of Blood Safety Research of Ministry of Health, Hangzhou 310006, Zhejiang Province, China.
Hang-Jun
HJ
Lyu
D000483:Alleles; D001483:Base Sequence; D005787:Gene Frequency; D005838:Genotype; D060005:Genotyping Techniques; D015236:HLA-C Antigens / Q000235:genetics*; D006239:Haplotypes; D006801:Humans
D015236:HLA-C Antigens