Mitochondrial P-glycoprotein ATPase contributes to insecticide resistance in the cotton bollworm, Helicoverpa armigera (Noctuidae: Lepidoptera).
Cotton bollworm, Helicoverpa armigera, is one of the most damaging polyphagous pests worldwide, which has developed high levels of resistance to commonly applied insecticides. Mitochondrial P-glycoprotein (Pgp) was detected in the insecticide-resistant strain of H. armigera using C219 antibodies, and its possible role was demonstrated in the efflux of xenobiotic compounds using spectrofluorometer. The TMR accumulated in mitochondria in the absence of ATP, and effluxed out in presence of ATP; the process of efflux was inhibited in the presence of ortho-vandate, an inhibitor of Pgp, in insecticide-resistant larvae of H. armigera. The mitochondria isolated from insecticide-resistant larvae were resistant to insecticide-induced inhibition of oxygen consumption and cytochrome c release. Membrane potential decreased in a dose-dependent manner in the presence of higher concentration of insecticides (>50 µM) in mitochondria of insecticide-resistant larvae. In conclusion, mitochondrial Pgp ATPase detected in the insecticide-resistant larvae influenced the efflux of xenobiotic compounds. Pgp might be involved in protecting the mitochondrial DNA and the components of the electron transport chain from damage due to insecticides, and contributing to the resistance to the deleterious effects of insecticides on the growth of insecticide-resistant H. armigera larvae.
Department of Biochemistry, Gulbarga University, Gulbarga, 585106, Karnataka, India.
S Md
SM
Akbar
Ravindra M
RM
Aurade
H C
HC
Sharma
K
K
Sreeramulu
D018435:ATP Binding Cassette Transporter, Subfamily B / Q000378:metabolism*; D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D001681:Biological Assay; D045304:Cytochromes c / Q000378:metabolism; D006575:Heterocyclic Compounds, 3-Ring / Q000378:metabolism; D007305:Insecticide Resistance; D007306:Insecticides / Q000633:toxicity; D007814:Larva / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D007915:Lepidoptera / Q000166:cytology* / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects* / Q000201:enzymology* / Q000378:metabolism; D010101:Oxygen Consumption / Q000187:drug effects; D012235:Rhodamines; D015262:Xenobiotics / Q000378:metabolism
D018435:ATP Binding Cassette Transporter, Subfamily B; D006575:Heterocyclic Compounds, 3-Ring; D007306:Insecticides; D012235:Rhodamines; D015262:Xenobiotics; C412715:tetramethylrosamine; D045304:Cytochromes c; D000251:Adenosine Triphosphatases