Yessotoxin triggers ribotoxic stress.
This work tests the hypothesis that the marine algal toxin yessotoxin (YTX) can trigger ribotoxic stress response in L6 and BC3H1 myoblast cells. YTX exposure at a concentration of 100 nM displays the characteristics of a ribotoxic stress response in such cells. The exposure leads to activation of the p38 mitogen-activated protein kinase, the stress-activated protein kinase c-jun, and the double-stranded RNA-activated protein kinase (PKR). YTX treatment also causes ribosomal RNA cleavage and inhibits protein synthesis. These observations support the idea that YTX can act as a ribotoxin.
Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences (NMBU), Campus Ås, P.O. Box 5003, NO-1432 ÅS, Norway. Electronic address: monica.suarez.korsnes@nmbu.no.
Mónica Suárez
MS
Korsnes
Norwegian University of Life Sciences (NMBU), Campus Adamstuen, P.O. Box 8146, NO-0033 OSLO, Norway.
Susan Skogtvedt
SS
Røed
Norwegian University of Life Sciences (NMBU), Campus Adamstuen, P.O. Box 8146, NO-0033 OSLO, Norway.
Michael A
MA
Tranulis
Norwegian University of Life Sciences (NMBU), Campus Adamstuen, P.O. Box 8146, NO-0033 OSLO, Norway.
Arild
A
Espenes
Norwegian University of Life Sciences (NMBU), Campus Adamstuen, P.O. Box 8146, NO-0033 OSLO, Norway.
Berit
B
Christophersen
D000818:Animals; D002460:Cell Line; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism; D008387:Marine Toxins / Q000633:toxicity*; D051379:Mice; D008978:Mollusk Venoms; D035964:Oxocins / Q000633:toxicity*; D014176:Protein Biosynthesis / Q000187:drug effects; D059367:RNA Cleavage; D012339:RNA, Ribosomal, 28S / Q000378:metabolism; D051381:Rats; D019892:eIF-2 Kinase / Q000378:metabolism; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
D008387:Marine Toxins; D008978:Mollusk Venoms; D035964:Oxocins; D012339:RNA, Ribosomal, 28S; D019892:eIF-2 Kinase; D048031:JNK Mitogen-Activated Protein Kinases; D048051:p38 Mitogen-Activated Protein Kinases; C066632:yessotoxin
28S rRNA cleavage; PKR; Protein synthesis inhibition; Ribotoxic stress response; Yessotoxin; c-jun