[Establishment of reverse-phase high-performance liquid chromatography with chiral reagent derivatization for separation of fexofenadine enantiomers].
To establish a precolumn chiral derivatization method for determination of fexofenadine enantiomers, a chiral substrate of OATP1B1, in cellular model.



R-(+)-phenylethyl isocyanate was selected as chiral derivatization reagent, which was reacted with fexofenadine to form carbamate derivatives. Enantiomers were identified by LC/MS and separated by RP-HPLC.



Under the experimental conditions, the fexofenadine enantiomers were separated completely. The standard curve was linear over the concentration range of 25-100 ng/ml (R(2)=0.9992, 0.9989). Accuracy was 101.1% and 98.3%, intra-precision was 2.4% and 3.1%, inter-precision was 3.1% and 4.0% for D1 and D2, respectively.



The method established is sensitive and accurate for determination of fexofenadine enantiomers in cells.
Laboratory of Pharmaceutical Analysis and Drug Metabolism, College of Pharmaceutical Sciences, Zhejiang Provincial Key Laboratory of Anti-Cancer Drug Research,Zhejiang University, Hangzhou 310058, China.
Qing-qing
QQ
Yao
Laboratory of Pharmaceutical Analysis and Drug Metabolism, College of Pharmaceutical Sciences, Zhejiang Provincial Key Laboratory of Anti-Cancer Drug Research,Zhejiang University, Hangzhou 310058, China.
Bo-xuan
BX
Qu
Laboratory of Pharmaceutical Analysis and Drug Metabolism, College of Pharmaceutical Sciences, Zhejiang Provincial Key Laboratory of Anti-Cancer Drug Research,Zhejiang University, Hangzhou 310058, China.
Quan
Q
Zhou
Laboratory of Pharmaceutical Analysis and Drug Metabolism, College of Pharmaceutical Sciences, Zhejiang Provincial Key Laboratory of Anti-Cancer Drug Research,Zhejiang University, Hangzhou 310058, China.
Su
S
Zeng
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D013237:Stereoisomerism; D016593:Terfenadine / Q000031:analogs & derivatives* / Q000032:analysis
D016593:Terfenadine; C093230:fexofenadine