Dissecting DR3 signaling.
Receptor signaling can be evaluated in multiple ways, including analysis of phosphorylation of downstream molecules and analysis of proteins that are recruited to the receptor upon ligand binding. Majority of studies on the mechanism of DR3 signaling were performed using overexpression systems that can often lead to artifacts. In this chapter we describe how to analyze DR3 downstream events with most attention being paid to endogenous immunoprecipitation.
Cell and Cancer Biology Branch, National Cancer Institute, National Institutes of Health, Bldg37, Rm1130, 37 Convent Dr., Bethesda, MD, 20892, USA.
Yelena L
YL
Pobezinskaya
Zhenggang
Z
Liu
Swati
S
Choksi
D015153:Blotting, Western / Q000379:methods*; D005452:Fluoresceins; D005456:Fluorescent Dyes; D006801:Humans; D047468:Immunoprecipitation / Q000379:methods*; D008213:Lymphocyte Activation / Q000187:drug effects; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D010766:Phosphorylation; D053219:Receptors, Tumor Necrosis Factor, Member 25 / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D013388:Succinimides
C087165:5-(6)-carboxyfluorescein diacetate succinimidyl ester; D005452:Fluoresceins; D005456:Fluorescent Dyes; D016328:NF-kappa B; D053219:Receptors, Tumor Necrosis Factor, Member 25; D013388:Succinimides; C506033:TNFRSF25 protein, human