[Quantitative determination of bosentan by proton nuclear magnetic resonance with internal standard method].
The study aims to establish a quantitative nuclear magnetic resonance (QNMR) method for the determination of the absolute content of bosentan. Proton nuclear magnetic resonance spectroscopy [1H NMR] spectra were obtained in CDCl3 with the internal standard dimethyl terephthalate and zg30 pulse sequence by using a Bruker AVANCE II 400 spectrometer. The content of bosentan is determined with QNMR in comparison with the result obtained by mass balance method. The result is 96.25% by QNMR and 96.54% by mass balance method. A rapid and accurate QNMR method has been established for the quantitative determination of the absolute content of bosentan. The study provides a new way for the quality control and calibration of a new reference standard material, it could be the complementary with the mass balance method for the assay of standard reference.
Cai-Yu
CY
Zhang
Na
N
Zhang
Lan
L
He
D000077300:Bosentan; D002138:Calibration; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D015394:Molecular Structure; D011522:Protons; D011786:Quality Control; D013449:Sulfonamides / Q000737:chemistry*
D011522:Protons; D013449:Sulfonamides; D000077300:Bosentan