Subacute antidiabetic and in vivo antioxidant effects of methanolic extract of Bridelia micrantha (Hochst Baill) leaf on alloxan-induced hyperglycaemic rats.
The methanolic leaf extract of Bridelia micrantha was tested for subacute antidiabetic and in vivo antioxidant effects in alloxan-induced hyperglycaemic rats. The subacute treatment of the extract (125, 250 and 500 mg/kg) produced 75, 68 and 63% reduction in fasting blood sugar level respectively, on day 14 of treatment. The extract produced time-dependent effect, but did not show a dose-dependent effect. Its optimum antidiabetic activity was noted at the dose of 125 mg/kg and this was comparable to glibenclamide 2 mg/kg (positive control). The extract (125 mg/kg) showed good oral glucose tolerance test (OGTT) effect in both normoglycaemic and hyperglycaemic rats. The OGTT effect of the extract (125 mg/kg) did not differ significantly (p>0.05) from glibenclamide (2 mg/kg). The antioxidant effect of the extract was assayed through the determination of the level of thiobarbituric acid reactive substance (TBARS) and catalase activity. The extract produced a dose-dependent decrease in the serum level of TBARS and gave its optimum catalase activity at the dose of 500 mg/kg. This study suggests that the B. micrantha extract has antihyperglycaemic and antioxidant activities. Therefore, could be a potential source of novel antidiabetic and antioxidant agent for the management of diabetes mellitus.
Yusuf Ndukaku
YN
Omeh
Samuel Okwudili
SO
Onoja
Maxwell Ikechukwu
MI
Ezeja
Peace Obiageli
PO
Okwor
D000496:Alloxan; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use*; D001786:Blood Glucose / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000188:drug therapy*; D004305:Dose-Response Relationship, Drug; D019665:Euphorbiaceae; D005951:Glucose Tolerance Test; D005905:Glyburide / Q000494:pharmacology / Q000627:therapeutic use; D006943:Hyperglycemia / Q000097:blood / Q000139:chemically induced / Q000188:drug therapy*; D007004:Hypoglycemic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D008297:Male; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D018515:Plant Leaves; D017208:Rats, Wistar; D012016:Reference Values; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D000975:Antioxidants; D001786:Blood Glucose; D007004:Hypoglycemic Agents; D010936:Plant Extracts; D017392:Thiobarbituric Acid Reactive Substances; D000496:Alloxan; D002374:Catalase; D005905:Glyburide