Evening primrose (Oenothera paradoxa) cake as an unconventional protein source.
An efficient procedure of a protein isolate production from the evening primrose cake was developed. The cake is a by-product of oil extraction from seeds by using the cold pressing method. The evening primrose cake contains 22.7% of protein. Its content in the protein isolate derived from the cake is 74%. Proteins present in evening primrose seeds are rich in Trp (7%) and Met (3%), but Lys-deficient (1.3%) as compared to the FAO protein standard. Apart from the proteins, the protein isolate contains 8.5% (w/w per s.s.) dietary fiber, that negatively affects its digestibility. To enhance the bio-availability of the protein isolate, it was partially hydrolyzed with commercial preparations of trypsin and other proteases (Alcalase and Flavourzyme, Novozymes. Denmark). The most advanced proteolysis (52%) was achieved by 6 h digestion of 2% protein suspension with a mixture of Flavourzyme and Alcalase (350 and 600 U per g of protein, respectively) at 50 degrees C and pH 9.0.
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Golabczak
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D000821:Animal Feed / Q000032:analysis; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D002417:Cattle; D004063:Digestion; D006868:Hydrolysis; D031664:Oenothera / Q000737:chemistry*; D010447:Peptide Hydrolases / Q000378:metabolism; D010936:Plant Extracts; D010940:Plant Proteins / Q000032:analysis / Q000378:metabolism*; D059748:Proteolysis; D051381:Rats; D012639:Seeds / Q000737:chemistry; D014357:Trypsin / Q000378:metabolism
D010936:Plant Extracts; D010940:Plant Proteins; D010447:Peptide Hydrolases; D014357:Trypsin