Isolation and antibiotic susceptibility of E. coli from urinary tract infections in a tertiary care hospital.
OBJECTIVE
The study was conducted to isolate and determine the antibiotic resistance in E. coli from urinary tract infections in a tertiary care hospital, Lahore.


METHODS
Urine samples (n=500) were collected from patients with signs and symptoms of Urinary tract infections. Bacteria were isolated and identified by conventional biochemical profile. Antibiotic resistance pattern of E. coli against different antibiotic was determined by Kirby-Baur method.


RESULTS
Bacterial etiological agent was isolated from 402 samples with highest prevalence of E. coli (321, 80%) followed by Staphylococcus aureus (9.4%), Proteus species (5.4%) and Pseudomonas species (5.2%). The E. coli were highly resistant to penicillin (100%), amoxicillin (100%) and cefotaxime (89.7%), followed by intermediate level of resistance to ceftazidime (73.8%), cephradine (73.8%), tetracycline (69.4%), doxycycline (66.6%), augmentin (62.6%), gentamycin (59.8%), cefuroxime (58.2%), ciprofloxacin (54.2%), cefaclor (50%), aztreonam (44.8%), ceftriaxone (43.3%), imipenem (43.3%), and low level of resistance to streptomycin (30%), kanamycin (19.9%), tazocin (14%), amikacin (12.7%) and lowest to norfloxacin (11.2%). Out of 321 E. coli isolates, 261 (81%) were declared as multiple drug resistant and 5 (1.5%) were extensive drug resistant.


CONCLUSIONS
It is concluded that most of the urinary tract infections in human are caused by multiple drug resistant E. coli.
Sumera Sabir, B.S (Microbiology), Department of Microbiology, Faculty of Veterinary Science, University of Veterinary and Animal Sciences, Lahore, Pakistan.
Sumera
S
Sabir
Aftab Ahmad Anjum, PhD (Microbiology), Department of Microbiology, Faculty of Veterinary Science, University of Veterinary and Animal Sciences, Lahore, Pakistan.
Aftab
A
Ahmad Anjum
Tayyaba Ijaz, PhD (Microbiology), Microbiology Diagnostic and Research Lab, Mayo Hospital, King Edward Medical University, Lahore, Pakistan.
Tayyaba
T
Ijaz
Muhammad Asad Ali, M. Phil (Microbiology), Department of Microbiology, Faculty of Veterinary Science, University of Veterinary and Animal Sciences, Lahore, Pakistan.
Muhammad
M
Asad Ali
Muti ur Rehman Khan, PhD (Pathology), Department of Pathology, Faculty of Veterinary Science, University of Veterinary and Animal Sciences, Lahore, Pakistan.
Muti
M
Ur Rehman Khan
Muhammad Nawaz, PhD (Medical Microbiology), Department of Microbiology, Faculty of Veterinary Science, University of Veterinary and Animal Sciences, Lahore, Pakistan.
Muhammad
M
Nawaz
Antibiotic resistance; E. coli; MDR; Prevalence; Urinary tract infections