[Genovariants of the cholera agent biovar El Tor: construction, molecular-genetic, and proteomic analysis].
Experimental modeling of origination of the virulent Vibrio cholerae El Tor genovariants is presented. It was demonstrated that the genovariants obtained did not differ from the natural genetically modified strains emerged in a natural population of the agent, either in phenotypical or genotypic properties. Using the PCR assay and sequencing techniques it was proved that the constructed genovariants carried a CTX(Class phi) prophage genome region with ctxBl gene of the V. cholerae classical biovar in the chromosome. It is shown that the prophage structure alterations lead to the increase in the toxigenicity and virulence in the genovariants compared to the typical strain-recipient. Moreover, as regards proteomics, changes in the expression of 26 proteins that perform various functions in the cell, such as metabolism, energy exchange, transportation, etc., were demonstrated. The data are indicative of the impact that a new DNA region in the genome of the genovariants has on the expression level of different house-keeping genes. The results obtained testify to the fact that one of the mechanisms of the genovariant emergence in the natural populations of the agent can be horizontal gene transfer.
N I
NI
Smirnova
D A
DA
Agafonov
E Yu
EY
Shchelkanova
S P
SP
Zadnova
A V
AV
Cherkasov
V V
VV
Kutyrev
D001483:Base Sequence; D002876:Chromosomes, Bacterial / Q000821:virology; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D039002:Prophages / Q000235:genetics; D020543:Proteome / Q000235:genetics* / Q000378:metabolism; D014734:Vibrio cholerae / Q000235:genetics* / Q000378:metabolism / Q000821:virology
D004269:DNA, Bacterial; D020543:Proteome