Far-red organic fluorophores contain a fluorescent impurity.
Far-red organic fluorophores commonly used in traditional and super-resolution localization microscopy are found to contain a fluorescent impurity with green excitation and near-red emission. This near-red fluorescent impurity can interfere with some multicolor stochastic optical reconstruction microscopy/photoactivated localization microscopy measurements in live cells and produce subtle artifacts in chemically fixed cells. We additionally describe alternatives to avoid artifacts in super-resolution localization microscopy.
Department of Biophysics, University of Michigan, 930 N University, Ann Arbor MI 48109 (USA).
Matthew B
MB
Stone
Sarah L
SL
Veatch
D000818:Animals; D016477:Artifacts; D045744:Cell Line, Tumor; D004396:Coloring Agents / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D007091:Image Processing, Computer-Assisted / Q000379:methods; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods
D004396:Coloring Agents; D005456:Fluorescent Dyes
fluorescence; fluorescent probes; live cells; quantitative imaging; super-resolution imaging