Further biodosimetry investigations using murine partial-body irradiation model.
This study evaluates both the effects of physical restraint and use of candidate biomarkers in a CD2F1 male mouse partial-body irradiation model for biological dosimetry diagnostic assays. Mice were irradiated (6-Gy, 250-kVp X ray) to 3/3rd (total body), 2/3rd (gut and torso), 1/3rd (gut only) and 0/3rd (sham) of total body. Blood was sampled for haematology and blood plasma proteomic biomarkers at 1 and 2 d after exposure. Increases in the body fraction exposed showed progressive decreases in lymphocyte counts and increases in the neutrophil-to-lymphocyte ratios with no significant differences in the neutrophil and platelet counts. The radioresponse for plasma biomarker Flt3L showed proportional increases; however, G-CSF and SAA levels exhibited dramatic and non-proportional increases in levels. Physical restraint at 1 d post-exposure increased lymphocyte counts and SAA, decreased neutrophil-to-lymphocyte ratio and Flt3L and showed no effects on neutrophil and platelet counts or G-CSF.
Armed Forces Radiobiology Research Institute, 8901 Wisconsin Avenue, Bethesda, MD 20889-5603, USA william.blakely@usuhs.edu.
W F
WF
Blakely
Armed Forces Radiobiology Research Institute, 8901 Wisconsin Avenue, Bethesda, MD 20889-5603, USA.
D J
DJ
Sandgren
Armed Forces Radiobiology Research Institute, 8901 Wisconsin Avenue, Bethesda, MD 20889-5603, USA.
V
V
Nagy
Armed Forces Radiobiology Research Institute, 8901 Wisconsin Avenue, Bethesda, MD 20889-5603, USA.
S-Y
SY
Kim
Meso Scale Diagnostics, Rockville, MD 20850, USA.
G B
GB
Sigal
Armed Forces Radiobiology Research Institute, 8901 Wisconsin Avenue, Bethesda, MD 20889-5603, USA.
N I
NI
Ossetrova
D000818:Animals; D001681:Biological Assay; D015415:Biomarkers / Q000032:analysis*; D001798:Blood Proteins / Q000032:analysis*; D005720:Gamma Rays; D008214:Lymphocytes / Q000378:metabolism / Q000528:radiation effects; D008297:Male; D051379:Mice; D009504:Neutrophils / Q000378:metabolism / Q000528:radiation effects; D020543:Proteome / Q000032:analysis* / Q000528:radiation effects*; D040901:Proteomics / Q000379:methods; D011829:Radiation Dosage; D011874:Radiometry
D015415:Biomarkers; D001798:Blood Proteins; D020543:Proteome