T-2 toxin, zearalenone and fumonisin B₁ in feedstuffs from China.
A total of 420 feedstuff samples were collected from China to determine and quantify T-2 toxin (T-2), zearalenone (ZEN) and fumonisin B₁ (FB₁). The contents of mycotoxins were determined by reverse-phase high-performance liquid chromatography with three-step cleanup and fluorescence detection. The mean recoveries of mycotoxins in spiked feedstuffs ranged from 72.2% to 95%. The limits of detection and quantification ranged from 1.8 to 5.7 and 6 to 19 µg/kg, respectively. Of the 420 analysed feedstuff samples, the incidence of T-2, ZEN and FB₁ was 79.5%, 85.2% and 96.1%, respectively; levels detected ranged from 10-735, 35-1478 and 20-6568 µg/kg, respectively. The results suggest a risk for domestic animals and humans. Further, the procedure was found to be suitable for determination of mycotoxins in feedstuffs and can be used for routine analysis. This is the first report in China on natural contamination of feedstuffs with these mycotoxins.
a College of Veterinary Medicine , Nanjing Agricultural University , Nanjing , China.
Ying
Y
Wang
Shengli
S
Liu
Hao
H
Zheng
Chenghua
C
He
Haibin
H
Zhang
D053000:Analytic Sample Preparation Methods; D000821:Animal Feed / Q000032:analysis* / Q000592:standards; D000818:Animals; D002273:Carcinogens / Q000032:analysis; D002645:Chickens; D002681:China; D002851:Chromatography, High Pressure Liquid; D056148:Chromatography, Reverse-Phase; D004968:Estrogens, Non-Steroidal / Q000032:analysis; D005506:Food Contamination; D005513:Food Inspection / Q000379:methods*; D037341:Fumonisins / Q000032:analysis*; D019983:Guideline Adherence; D007166:Immunosuppressive Agents / Q000032:analysis; D057230:Limit of Detection; D011042:Poisons / Q000032:analysis*; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence; D034421:Sus scrofa; D013605:T-2 Toxin / Q000032:analysis*; D015025:Zearalenone / Q000032:analysis*
D002273:Carcinogens; D004968:Estrogens, Non-Steroidal; D037341:Fumonisins; D007166:Immunosuppressive Agents; D011042:Poisons; C056933:fumonisin B1; D015025:Zearalenone; D013605:T-2 Toxin
China; HPLC; T-2 toxin; feedstuffs; fumonisin B1; zearalenone