Aflatoxin in raw walnut kernels marketed in Tehran, Iran.
The occurrence of aflatoxin in 35 samples of raw walnuts, which were randomly collected from Iranian supermarkets in Tehran, was examined. Analysis of aflatoxin was performed by solvent extraction and immunoaffinity clean-up followed by determination using high-performance liquid chromatography with fluorescence detection. Limits of detection (LOD) for the aflatoxins were 0.03-0.25 ng g⁻¹. Limits of quantification (LOQ) were 0.12-0.80 ng g⁻¹. Total aflatoxin concentrations in the samples ranged from 0 to 112.8 ng g⁻¹. About 74.3% of the samples were contaminated with aflatoxins, of which 20.0% exceeded the maximum tolerable limit of 15 ng g⁻¹ set by Codex.
a Food and Drug Research Laboratory Center, Food and Drug Organization , Tehran , Iran.
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Imani Nejad
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Farahani
D016604:Aflatoxin B1 / Q000032:analysis; D000348:Aflatoxins / Q000032:analysis*; D053000:Analytic Sample Preparation Methods; D002273:Carcinogens / Q000032:analysis*; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D005506:Food Contamination; D005513:Food Inspection; D019983:Guideline Adherence; D006291:Health Policy; D006293:Health Promotion; D006801:Humans; D007492:Iran; D031324:Juglans / Q000737:chemistry*; D057230:Limit of Detection; D009754:Nuts / Q000737:chemistry* / Q000191:economics; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence; D014944:World Health Organization
D000348:Aflatoxins; D002273:Carcinogens; C029753:aflatoxin B2; D016604:Aflatoxin B1
HPLC with flourescence; aflatoxins; cereals and grain; walnut