Efficient removal of pathogenic bacteria and viruses by multifunctional amine-modified magnetic nanoparticles.
A novel amine-functionalized magnetic Fe3O4-SiO2-NH2 nanoparticle was prepared by layer-by-layer method and used for rapid removal of both pathogenic bacteria and viruses from water. The nanoparticles were characterized by TEM, EDS, XRD, XPS, FT-IR, BET surface analysis, magnetic property tests and zeta-potential measurements, respectively, which demonstrated its well-defined core-shell structures and strong magnetic responsivity. Pathogenic bacteria and viruses are often needed to be removed conveniently because of a lot of co-existing conditions. The amine-modified nanoparticles we prepared were attractive for capturing a wide range of pathogens including not only bacteriophage f2 and virus (Poliovirus-1), but also various bacteria such as S. aureus, E. coli O157:H7, P. aeruginosa, Salmonella, and B. subtilis. Using as-prepared amine-functionalized MNPs as absorbent, the nonspecific removal efficiency of E. coli O157:H7 or virus was more than 97.39%, while it is only 29.8% with Fe3O4-SiO2 particles. From joint removal test of bacteria and virus, there are over 95.03% harmful E. coli O157:H7 that can be removed from mixed solution with polyclonal anti-E. coli O157:H7 antibody modified nanoparticles. Moreover, the synergy effective mechanism has also been suggested.
College of Environmental Science and Engineering, Nankai University, Tianjin 300071, PR China; Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN 46556, United States. Electronic address: sihuizhan@nankai.edu.cn.
Sihui
S
Zhan
College of Environmental Science and Engineering, Nankai University, Tianjin 300071, PR China.
Yang
Y
Yang
Key Laboratory of Risk Assessment and Control for Environment and Food Safety, Institute of Health and Environmental Medicine, Tianjin 300050, PR China.
Zhiqiang
Z
Shen
Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN 46556, United States.
Junjun
J
Shan
Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN 46556, United States; Department of Chemistry, Tianjin University, Tianjin 300072, PR China.
Yi
Y
Li
Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN 46556, United States.
Shanshan
S
Yang
College of Environmental Science and Engineering, Nankai University, Tianjin 300071, PR China.
Dandan
D
Zhu
D000327:Adsorption; D000588:Amines / Q000737:chemistry*; D000907:Antibodies, Bacterial / Q000737:chemistry; D001419:Bacteria / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D060328:Magnetic Phenomena; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D017955:Poliovirus / Q000737:chemistry / Q000302:isolation & purification*; D012822:Silicon Dioxide / Q000737:chemistry*; D014873:Water Pollutants / Q000737:chemistry / Q000302:isolation & purification*; D018508:Water Purification / Q000379:methods
D000588:Amines; D000907:Antibodies, Bacterial; D058185:Magnetite Nanoparticles; D014873:Water Pollutants; D012822:Silicon Dioxide
Magnetic core/shell nanoparticles; Pathogenic bacteria and viruses; Surface functionalizations