Methodological considerations for heat shock of the nematode Caenorhabditis elegans.
Stress response pathways share commonalities across many species, including humans, making heat shock experiments valuable tools for many biologists. The study of stress response in Caenorhabditis elegans has provided great insight into many complex pathways and diseases. Nevertheless, the heat shock/heat stress field does not have consensus as to the timing, temperature, or duration of the exposure and protocols differ extensively between laboratories. The lack of cohesiveness makes it difficult to compare results between groups or to know where to start when preparing your own protocol. We present a discussion of some of the major hurdles to reproducibility in heat shock experiments as well as detailed protocols for heat shock and hormesis experiments.
Magee-Womens Research Institute, University of Pittsburgh School of Medicine, 204 Craft Avenue, Pittsburgh, PA 15213, USA.
Shannin C
SC
Zevian
Magee-Womens Research Institute, University of Pittsburgh School of Medicine, 204 Craft Avenue, Pittsburgh, PA 15213, USA. Electronic address: jly@alum.mit.edu.
Judith L
JL
Yanowitz
D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics* / Q000502:physiology; D018869:Heat-Shock Response / Q000235:genetics*; D059165:Hormesis; D006358:Hot Temperature; D006801:Humans; D015398:Signal Transduction / Q000235:genetics; D013312:Stress, Physiological / Q000235:genetics*
Heat shock; Heat stress; Hormesis