Topical application of retinyl palmitate-loaded nanotechnology-based drug delivery systems for the treatment of skin aging.
The objective of this study was to perform a structural characterization and evaluate the in vitro safety profile and in vitro antioxidant activity of liquid crystalline systems (LCS) with and without retinyl palmitate (RP). LCS containing polyether functional siloxane (PFS) as a surfactant, silicon glycol copolymer (SGC) as oil phase, and water in the ratios 30 : 25 : 45 and 40 : 50 : 10 with (OLS(v) = RP-loaded opaque liquid system and TLS(v) = RP-loaded transparent liquid system, respectively) and without (OLS and TLS, respectively) RP were studied. Samples were characterized using polarized light microscopy (PLM) and rheology analysis. In vitro safety profile was evaluated using red cell hemolysis and in vitro cytotoxicity assays. In vitro antioxidant activity was performed by the DPPH method. PLM analysis showed the presence of lamellar LCS just to TLS. Regardless of the presence of RP, the rheological studies showed the pseudoplastic behavior of the formulations. The results showed that the incorporation of RP in LCS improved the safety profile of the drug. In vitro antioxidant activity suggests that LCS presented a higher capacity to maintain the antioxidant activity of RP. PFS-based systems may be a promising platform for RP topical application for the treatment of skin aging.
Departament of Drugs and Pharmaceuticals, School of Pharmaceutical Sciences, UNESP, 14801-902 Araraquara, SP, Brazil.
Marcela B
MB
Oliveira
Departament of Drugs and Pharmaceuticals, School of Pharmaceutical Sciences, UNESP, 14801-902 Araraquara, SP, Brazil.
Alice Haddad
AH
do Prado
Departament of Drugs and Pharmaceuticals, School of Pharmaceutical Sciences, UNESP, 14801-902 Araraquara, SP, Brazil.
Jéssica
J
Bernegossi
Departament of Drugs and Pharmaceuticals, School of Pharmaceutical Sciences, UNESP, 14801-902 Araraquara, SP, Brazil.
Claudia S
CS
Sato
Departament of Clinical Analysis, School of Pharmaceutical Sciences, UNESP, 14801-902 Araraquara, SP, Brazil, Brazil.
Iguatemy
I
Lourenço Brunetti
Departament of Drugs and Pharmaceuticals, School of Pharmaceutical Sciences, UNESP, 14801-902 Araraquara, SP, Brazil.
Maria Virgínia
MV
Scarpa
Institute of Environmental, Chemistry and Pharmaceuticals Sciences, Federal University of São Paulo (UNIFESP), 09972-270 Diadema, SP, Brazil.
Gislaine Ricci
GR
Leonardi
Ugelstad Laboratory, Norwegian University of Science and Technology (NTNU), 7491 Trondheim, Norway.
Stig E
SE
Friberg
Departament of Drugs and Pharmaceuticals, School of Pharmaceutical Sciences, UNESP, 14801-902 Araraquara, SP, Brazil.
Marlus
0000-0002-6698-0545
M
Chorilli
D000287:Administration, Topical; D004224:Diterpenes; D016503:Drug Delivery Systems; D004353:Drug Evaluation, Preclinical; D006801:Humans; D036103:Nanotechnology; D000084562:Retinyl Esters; D015595:Skin Aging / Q000187:drug effects*; D014801:Vitamin A / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology
D004224:Diterpenes; D000084562:Retinyl Esters; D014801:Vitamin A; C014794:retinol palmitate