Influence of extraction solvent on antioxidant capacity value of oleaster measured by ORAC method.
Oxygen radical absorbance capacity (ORAC) is a widely used hydrogen atom transfer-based method which measures the antioxidant capacity of natural products. ORAC values of oleaster (Elaeagnus angustifolia L.), which was extracted with ethanol/acetone (7:3, v/v), ethanol/water (1:1, v/v) and methanol/water (1:1, v/v) in order to evaluate the effects of solvent type on antioxidant capacity, were examined. In general, results revealed that ethanol/water extracts exhibited better antioxidant capacity values. Furthermore, results obtained by using ORAC-eosin y (ORAC-EY), one of the widely used derivative of fluorescein (FL), as a fluorescent probe were compared with those obtained by using ORAC-FL. According to the results, ORAC-EY values were found to be compatible with ORAC-FL values.
a Department of Analytical Chemistry , Faculty of Pharmacy, Ege University , 35100 Bornova , Izmir , Turkey.
Gorkem
G
Yalcin
Ozlem
O
Sogut
D000975:Antioxidants / Q000032:analysis / Q000494:pharmacology*; D031263:Elaeagnaceae / Q000737:chemistry*; D000431:Ethanol; D019793:Fluorescein / Q000737:chemistry; D005452:Fluoresceins / Q000032:analysis; D005456:Fluorescent Dyes / Q000032:analysis; D017382:Reactive Oxygen Species; D014867:Water
D000975:Antioxidants; D005452:Fluoresceins; D005456:Fluorescent Dyes; D017382:Reactive Oxygen Species; C587846:fluorescein (3',6'-dihydroxyspiro(isobenzofuran-1(3H), 9'(9H)-xanthen)-3-one); D014867:Water; D000431:Ethanol; D019793:Fluorescein
Elaeagnus angustifolia L.; ORAC; antioxidant capacity