Physicochemical, mechanical, and molecular properties of nonlysogenic and p22-lysogenic Salmonella typhimurium treated with citrus oil.
The aim of this study was to evaluate the phenotypic and genotypic properties of nonlysogenic Salmonella Typhimurium (ST(P22-)) and lysogenic Salmonella Typhimurium (ST(P22+)) in the presence of sublethal concentrations (SLC2D) of citrus essential oils (CEOs), which were used to evaluate antimicrobial susceptibility, cell surface hydrophobicity, autoaggregation ability, bacterial motility, lysogenic conversion, gene expression patterns, and antibiofilm formation. The SLC2D values of non-heat-treated (N-CEO) and heat-treated (H-CEO) CEO in an autoclave at 121°C for 20 min were 2.0 to 2.1 mg/ml against ST(P22-) and 1.7 to 1.9 mg/ml against STP(22+). The rates of injured ST(P22-) and ST(P22+) cells treated with SLC2D of N-CEO and H-CEO ranged from 67 to 83%. The hydrophobicity and autoaggregation were decreased to 2.5 and 19.5% for ST(P22-) and 4.7 and 21.7% for ST(P22+), respectively, in the presence of N-CEO. A noticeable reduction in the swarming motility was observed in ST(P22-) with N-CEO (14.5%) and H-CEO (13.3%). The numbers of CEO-induced P22 were 5.40 log PFU/ml for N-CEO and 5.65 log PFU/ml for H-CEO. The relative expression of hilA, hilC, hilD, invA, invC, invE, invF, sirA, and sirB was down-regulated in ST(P22-) and ST(P22+) with N-CEO and H-CEO. The numbers of adherent ST(P22-) and ST(P22+) were effectively reduced by more than 1 log in the presence of CEO. These results suggest that CEO has potential to be used to control bacterial attachment, colonization, and invasion.
Department of Medical Biomaterials Engineering, Kangwon National University, Chuncheon, Gangwon 200-701, Republic of Korea. juheeahn@kangwon.ac.kr.
Juhee
J
Ahn
Department of Animal and Avian Sciences, University of Maryland, College Park, Maryland 20742, USA.
Jose Alejandro
JA
Almario
Department of Animal and Avian Sciences, University of Maryland, College Park, Maryland 20742, USA.
Serajus
S
Salaheen
Department of Animal and Avian Sciences, University of Maryland, College Park, Maryland 20742, USA. dbiswas@umd.edu.
Debabrata
D
Biswas
D001426:Bacterial Proteins / Q000235:genetics; D017100:Bacteriophage P22 / Q000502:physiology*; D002957:Citrus / Q000737:chemistry*; D015536:Down-Regulation / Q000187:drug effects; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D008242:Lysogeny; D009822:Oils, Volatile / Q000494:pharmacology*; D010938:Plant Oils / Q000494:pharmacology*; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000821:virology*
D001426:Bacterial Proteins; D009822:Oils, Volatile; D010938:Plant Oils