Influence of the observer's level of experience on systolic and diastolic arterial blood pressure measurements using Doppler ultrasonography in healthy conscious cats.
The objective of this study was to determine the influence of the observer's level of experience on within- and between-day variability, and the percentage of successful systolic (SAP) and diastolic arterial blood pressure (DAP) measurements obtained by Doppler ultrasonography (DU) in awake cats. For this purpose, six healthy conscious cats were used and four observers with different levels of training performed 144 SAP and DAP measurements on 4 days using DU. Measurements were recorded five consecutive times, and mean values were used for statistical analysis. Only the two most skilled observers - a PhD student in cardiology and a Dipl ECVIM-CA (cardiology) - had within- and between-day coefficients of variation (CVs) for SAP ⩽16% (13-16%). Conversely, the two less experienced observers - a fifth-year student and an assistant - had high between-day CVs (61% and 73%). For DAP, only the most experienced observer (Dipl ECVIM-CA) succeeded in 100% of the attempts, with within- and between-day CVs of 11% and 4%, respectively. Conversely, DAP could not be measured by the other three observers in 8%, 19% and 56% of attempts (from the highest to the lowest level of experience); therefore, the corresponding CV values could not be calculated. In conclusion, SAP may be assessed using DU in healthy awake cats with good repeatability and reproducibility by a well-trained observer. Measurement of DAP is more difficult than of SAP, and needs a longer training period, which represents one of the limitations of DU in cats.
Alfort Cardiology Unit (UCA), Central University Veterinary Hospital of Alfort (CHUVA), National Veterinary School of Alfort, Paris-Est University, Maisons-Alfort, France INSERM U955, Equipe 3, Créteil, France.
Vassiliki
V
Gouni
INSERM U955, Equipe 3, Créteil, France Unit of Pharmatoxicology, National Veterinary School of Alfort, Paris-Est University, Maisons-Alfort, France.
Renaud
R
Tissier
Alfort Cardiology Unit (UCA), Central University Veterinary Hospital of Alfort (CHUVA), National Veterinary School of Alfort, Paris-Est University, Maisons-Alfort, France.
Charlotte
C
Misbach
Alfort Cardiology Unit (UCA), Central University Veterinary Hospital of Alfort (CHUVA), National Veterinary School of Alfort, Paris-Est University, Maisons-Alfort, France.
David
D
Balouka
Alfort Cardiology Unit (UCA), Central University Veterinary Hospital of Alfort (CHUVA), National Veterinary School of Alfort, Paris-Est University, Maisons-Alfort, France.
Hanna
H
Bueno
Alfort Cardiology Unit (UCA), Central University Veterinary Hospital of Alfort (CHUVA), National Veterinary School of Alfort, Paris-Est University, Maisons-Alfort, France INSERM U955, Equipe 3, Créteil, France.
Jean-Louis
JL
Pouchelon
Clinical Research Unit, National Veterinary School of Toulouse, University of Toulouse, INP, Toulouse, France.
Hervé P
HP
Lefebvre
Alfort Cardiology Unit (UCA), Central University Veterinary Hospital of Alfort (CHUVA), National Veterinary School of Alfort, Paris-Est University, Maisons-Alfort, France INSERM U955, Equipe 3, Créteil, France vchetboul@vet-alfort.fr.
Valérie
V
Chetboul
D000818:Animals; D001794:Blood Pressure / Q000502:physiology; D001795:Blood Pressure Determination / Q000379:methods / Q000662:veterinary*; D002415:Cats / Q000502:physiology*; D003243:Consciousness; D009991:Oscillometry / Q000662:veterinary; D012016:Reference Values; D015203:Reproducibility of Results; D018608:Ultrasonography, Doppler / Q000379:methods / Q000662:veterinary*