Study of effect of salvianolic acid B on motor function recovery in rats with spinal cord injury.
In this study effect of salvianolic acid B was observed on motor function recovery of rats with spinal cord injury. 50 rats were selected and after inducing SCI their recovery under controlled conditions was studied using Sal B and PBS (as control). Both compounds were introduced intraperitoneally in respective groups of traumatic rats at the same time intervals for 28 days. It was observed that Sal B introduced at 5  mg/kg/day resulted in better motor function recovery. BBB score was recorded which increased significantly along with the reduction in cavity area observed by bright field microscopy of tissues, that is, from 1 to 10 and from 0.20 ± 0.05 mm(2) to 0.10 ± 0.03 mm(2), in Sal B treated group, respectively, compared to PBS group. Statistical analysis was carried out using SPSS software (SPSS, Chicago, IL, USA), values were expressed as mean ± SEM, and P value <0.01 was considered significant. Effect of Sal B on expression of NF-kB p65 and IkB α was studied and OD values of densitometry of western blots were taken. MPO activity was also studied. It was observed that treatment of Sal B significantly reduced the expression of both compounds in Sal B treated group as compared to control group after 28 days of treatment.
Department of Orthopedic Surgery, First Affiliated Hospital of Dalian Medical University, Zhongshan Road, No. 222, Dalian, Liaoning 116000, China.
Chong
C
Xun
Department of Orthopedic Surgery, First Affiliated Hospital of Dalian Medical University, Zhongshan Road, No. 222, Dalian, Liaoning 116000, China.
Yang
Y
Hu
Department of Orthopedic Surgery, First Affiliated Hospital of Dalian Medical University, Zhongshan Road, No. 222, Dalian, Liaoning 116000, China.
Ming
M
Lu
Department of Orthopedic Surgery, First Affiliated Hospital of Dalian Medical University, Zhongshan Road, No. 222, Dalian, Liaoning 116000, China.
Shouyu
S
Wang
Department of Orthopedic Surgery, First Affiliated Hospital of Dalian Medical University, Zhongshan Road, No. 222, Dalian, Liaoning 116000, China.
Decheng
0000-0002-1165-3723
D
Lv
D000818:Animals; D002109:Caffeic Acids / Q000494:pharmacology*; D005260:Female; D020672:I-kappa B Proteins / Q000378:metabolism; D007773:Lactates / Q000494:pharmacology*; D009043:Motor Activity / Q000187:drug effects*; D000072000:NF-KappaB Inhibitor alpha; D009419:Nerve Tissue Proteins / Q000378:metabolism; D054328:Proton Pump Inhibitors / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013119:Spinal Cord Injuries / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D013997:Time Factors; D051996:Transcription Factor RelA / Q000378:metabolism
D002109:Caffeic Acids; D020672:I-kappa B Proteins; D007773:Lactates; D009419:Nerve Tissue Proteins; C000606206:Nfkbia protein, rat; D054328:Proton Pump Inhibitors; C498145:Rela protein, rat; D051996:Transcription Factor RelA; D000072000:NF-KappaB Inhibitor alpha; C066201:salvianolic acid A