Hesperidin increases intestinal β,β-carotene 15-15' mono-oxygenase 1 (BCMO1) activity in Mongolian gerbils (Meriones unguiculatus) fed with β-carotene-free diet.
β,β-Carotene 15-15' mono-oxygenase 1 (BCMO1) is a key enzyme in vitamin A (VitA) metabolism in mammals. Dietary compounds, such as carotenoids and polyphenols, were reported to influence BCMO1 activity. The aim of this study was to evaluate the effect of hesperidin (Hes), on the VitA bioefficacy of β-carotene (Bc) from orange-fleshed sweet potato, using Mongolian gerbils, focussing on BCMO1 activity. Gerbils (n=50) depleted in VitA were divided into five groups fed with basal diet containing 3% white- or orange-fleshed sweet potatoes supplemented or not with Hes. Liver BCMO1 activity was low, with no significant differences between groups. Interestingly, intestinal mucosal BCMO1 activity was significantly higher in the gerbils fed without Bc or VitA than those fed with a VitA/Bc-supplemented diet. Finally, our results show that, under a low VitA status, Hes dramatically stimulated intestinal BCMO1 activity, an effect that could possibly be related to its action as an agonist of PPARγ.
Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), Département PERSYST, UMR Qualisud, TA B-95/16, 34398 Montpellier, France.
Marie
M
Poulaert
Université Montpellier II, UMR Qualisud, 34095 Montpellier Cedex 5, France.
Ziya
Z
Gunata
Lab PMOI, Université de Lille2, Faculté de Chirurgie Dentaire, 59000 Lille, France.
Alexandrine
A
During
INRA, UMR1260, National Institute for Agronomic Research, Nutrition, Obesity and Risk of Thrombosis, Marseille F-13385, France; INSERM, UMR1062, National Institute of Health and Medical Research, Marseille F-13385, France; Aix-Marseille University, Marseille F-13385, France.
Emmanuelle
E
Reboul
Université Montpellier II, UMR 204 Nutripass, 34095 Montpellier Cedex 5, France.
Caroline
C
Laurent
Université Montpellier II, UMR 204 Nutripass, 34095 Montpellier Cedex 5, France.
Sylvie
S
Gaillet
Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), Département PERSYST, UMR Qualisud, TA B-95/16, 34398 Montpellier, France. Electronic address: claudie.dhuique-mayer@cirad.fr.
Claudie
C
Dhuique-Mayer
D000821:Animal Feed; D000818:Animals; D002338:Carotenoids / Q000737:chemistry; D019587:Dietary Supplements; D005786:Gene Expression Regulation; D005849:Gerbillinae; D006569:Hesperidin / Q000737:chemistry*; D007422:Intestines / Q000201:enzymology*; D027723:Ipomoea batatas / Q000378:metabolism; D008099:Liver / Q000201:enzymology; D008297:Male; D010105:Oxygenases; D012176:Retinoids / Q000737:chemistry; D014801:Vitamin A / Q000737:chemistry*; D019207:beta Carotene / Q000737:chemistry; D050559:beta-Carotene 15,15'-Monooxygenase / Q000737:chemistry*
D012176:Retinoids; D019207:beta Carotene; D014801:Vitamin A; D002338:Carotenoids; D006569:Hesperidin; D010105:Oxygenases; D050559:beta-Carotene 15,15'-Monooxygenase
Orange-fleshed sweet potato; Retinoid; Scavenger receptor B type 1; Vitamin A status