Roles of sphincter of Oddi motility and serum vasoactive intestinal peptide, gastrin and cholecystokinin octapeptide.
OBJECTIVE
To investigate roles of sphincter of Oddi (SO) motility played in pigment gallbladder stone formation in model of guinea pigs.


METHODS
Thirty-four adult male Hartley guinea pigs were divided randomly into two groups: the control group and pigment stone group. The pigment stone group was divided into 4 subgroups with 6 guinea pigs each according to time of sacrifice, and were fed a pigment lithogenic diet and sacrificed after 3, 6, 9 and 12 wk. SO manometry and recording of myoelectric activity of the guinea pigs were obtained by multifunctional physiograph at each stage. Serum vasoactive intestinal peptide (VIP), gastrin and cholecystokinin octapeptide (CCK-8) were detected at each stage in the process of pigment gallbladder stone formation by enzyme-linked immunosorbent assay.


RESULTS
The incidence of pigment gallstone formation was 0%, 0%, 16.7% and 66.7% in the 3-, 6-, 9- and 12-wk group, respectively. The frequency of myoelectric activity decreased in the 3-wk group. The amplitude of myoelectric activity had a tendency to decrease but not significantly. The frequency of the SO decreased significantly in the 9-wk group. The SO basal pressure and common bile duct pressure increased in the 12-wk group (25.19 ± 7.77 mmHg vs 40.56 ± 11.81 mmHg, 22.35 ± 7.60 mmHg vs 38.51 ± 11.57 mmHg, P < 0.05). Serum VIP was significantly elevated in the 6- and 12-wk groups and serum CCK-8 was decreased significantly in the 12-wk group.


CONCLUSIONS
Pigment gallstone-causing diet may induce SO dysfunction. The tension of the SO increased. The disturbance in SO motility may play a role in pigment gallstone formation, and changes in serum VIP and CCK-8 may be important causes of SO dysfunction.
Zhen-Hai Zhang, Cheng-Kun Qin, Jian Xu, Xian-Ping Cui, Zhi-Yi Wang, Guo-Zhe Xian, Department of Hepatobiliary Surgery, Shandong Provincial Hospital Affiliated to Shangdong University, Jinan 250021, Shandong Province, China.
Zhen-Hai
ZH
Zhang
Zhen-Hai Zhang, Cheng-Kun Qin, Jian Xu, Xian-Ping Cui, Zhi-Yi Wang, Guo-Zhe Xian, Department of Hepatobiliary Surgery, Shandong Provincial Hospital Affiliated to Shangdong University, Jinan 250021, Shandong Province, China.
Cheng-Kun
CK
Qin
Zhen-Hai Zhang, Cheng-Kun Qin, Jian Xu, Xian-Ping Cui, Zhi-Yi Wang, Guo-Zhe Xian, Department of Hepatobiliary Surgery, Shandong Provincial Hospital Affiliated to Shangdong University, Jinan 250021, Shandong Province, China.
Shuo-Dong
SD
Wu
Zhen-Hai Zhang, Cheng-Kun Qin, Jian Xu, Xian-Ping Cui, Zhi-Yi Wang, Guo-Zhe Xian, Department of Hepatobiliary Surgery, Shandong Provincial Hospital Affiliated to Shangdong University, Jinan 250021, Shandong Province, China.
Jian
J
Xu
Zhen-Hai Zhang, Cheng-Kun Qin, Jian Xu, Xian-Ping Cui, Zhi-Yi Wang, Guo-Zhe Xian, Department of Hepatobiliary Surgery, Shandong Provincial Hospital Affiliated to Shangdong University, Jinan 250021, Shandong Province, China.
Xian-Ping
XP
Cui
Zhen-Hai Zhang, Cheng-Kun Qin, Jian Xu, Xian-Ping Cui, Zhi-Yi Wang, Guo-Zhe Xian, Department of Hepatobiliary Surgery, Shandong Provincial Hospital Affiliated to Shangdong University, Jinan 250021, Shandong Province, China.
Zhi-Yi
ZY
Wang
Zhen-Hai Zhang, Cheng-Kun Qin, Jian Xu, Xian-Ping Cui, Zhi-Yi Wang, Guo-Zhe Xian, Department of Hepatobiliary Surgery, Shandong Provincial Hospital Affiliated to Shangdong University, Jinan 250021, Shandong Province, China.
Guo-Zhe
GZ
Xian
D000818:Animals; D002779:Cholestasis / Q000097:blood / Q000209:etiology* / Q000503:physiopathology; D004195:Disease Models, Animal; D042882:Gallstones / Q000097:blood / Q000209:etiology* / Q000503:physiopathology; D005755:Gastrins / Q000097:blood*; D006168:Guinea Pigs; D008297:Male; D008365:Manometry; D008564:Membrane Potentials; D011312:Pressure; D012844:Sincalide / Q000097:blood*; D009803:Sphincter of Oddi / Q000378:metabolism / Q000503:physiopathology*; D013997:Time Factors; D014660:Vasoactive Intestinal Peptide / Q000097:blood*
D005755:Gastrins; D014660:Vasoactive Intestinal Peptide; D012844:Sincalide
Cholecystokinin octapeptide; Gastrin; Guinea pig; Manometry; Myoelectric activity; Pigment gallstone; Sphincter of Oddi; Vasoactive intestinal peptide