Human gingiva-derived mesenchymal stromal cells contribute to periodontal regeneration in beagle dogs.
Gingiva-derived mesenchymal stromal cells (GMSCs) have been considered as a promising alternative strategy for periodontal regeneration based on their potential for multilineage differentiation in vitro and the ability to form new bone in vivo. In order to investigate the capacity of GMSCs for periodontal regeneration and the fate of GMSCs during periodontal tissue repair, enhanced green fluorescent protein-labeled GMSCs were transplanted into class III furcation defects created in beagle dogs. The results showed that the transplanted GMSCs significantly enhanced the regeneration of the damaged periodontal tissue, including the alveolar bone, cementum and functional periodontal ligament (PDL). Moreover, GMSCs were able to differentiate into osteoblasts, cementoblasts and PDL fibroblasts in vivo. These findings indicate that GMSCs represent a novel cell source for periodontal tissue reconstruction.
Department of Periodontology, School of Stomatology, Shandong University, Jinan, China.
Xinbo
X
Yu
Shaohua
S
Ge
Shulan
S
Chen
Quanchen
Q
Xu
Jin
J
Zhang
Hongmei
H
Guo
Pishan
P
Yang
D000293:Adolescent; D000328:Adult; D000818:Animals; D001861:Bone Regeneration; D002454:Cell Differentiation; D064987:Cell- and Tissue-Based Therapy / Q000379:methods*; D003739:Dental Cementum / Q000166:cytology; D004285:Dogs; D005881:Gingiva / Q000166:cytology*; D049452:Green Fluorescent Proteins; D016557:Guided Tissue Regeneration, Periodontal; D006801:Humans; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology; D010012:Osteogenesis; D010513:Periodontal Ligament / Q000166:cytology*; D010518:Periodontitis / Q000628:therapy; D014183:Transplantation, Heterologous; D014945:Wound Healing; D055815:Young Adult
C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins