A roadmap to the isotopolog space of flavocoenzymes.
Flavocoenzymes with selective or universal stable isotope labeling are important tools for the investigation of flavoproteins using a variety of spectroscopic methods. Numerous selectively labeled flavin isotopologs can be generated by the combined application of chemical synthesis and in vitro biotransformation using commercially available enzymes and/or recombinant riboflavin biosynthesis enzymes. Notably, the complex reaction sequences can be rapidly carried out using enzyme-assisted one-pot reaction strategies.
Department of Chemistry, Organic Chemistry & Biochemistry, Technische Universität München, Lichtenbergstraße 4, 85748 Garching, Munich, Germany, adelbert.bacher@t-online.de.
Adelbert
A
Bacher
Boris
B
Illarionov
Wolfgang
W
Eisenreich
Markus
M
Fischer
D001463:Barbiturates / Q000737:chemistry; D001711:Biotransformation; D003067:Coenzymes / Q000138:chemical synthesis / Q000378:metabolism*; D005415:Flavins / Q000138:chemical synthesis / Q000378:metabolism*; D007553:Isotope Labeling; D011621:Pteridines / Q000378:metabolism; D012256:Riboflavin / Q000138:chemical synthesis / Q000378:metabolism
D001463:Barbiturates; D003067:Coenzymes; D005415:Flavins; D011621:Pteridines; C001061:6,7-dimethyl-8-ribityllumazine; D012256:Riboflavin; C032232:barbituric acid