[Relation of Treg and iNKT cell reconstruction with aGVHD after allogeneic hematopoietic stem cell transplantation in children].
This study was aimed to explore the relation of Treg and invariant natural killer T (iNKT) cell reconstruction with acute graft-versus-host disease (aGVHD) after allogeneic hematopoietic stem cell transplantation (allo-HSCT) in children. According to the occurrence or absence of aGVHD, 29 pediatric patients who underwent allo-HSCT were firstly divided into two groups non-aGVHD and aGVHD group,then those patients with aGVHD were divided into steroid effective group and steroid resistant group according to their reaction to the steroid treatment. Flow cytometry was used to detect the frequency of Treg cells and iNKT cells in the peripheral blood of the recipients at different time after allo-HSCT(d 15, d 30, d 60, d 90, the time of aGVHD onset and two weeks after steroid treatment). The result showed that the frequencies of Treg cells and the iNKT/T ratio on day 15 in non-aGVHD group were significantly higher than those in the aGVHD group (P < 0.05). It is concluded that a combined monitoring strategy of Treg and iNKT cell reconstruction early after allo-HSCT may facilitate the diagnosis and treatment of aGVHD in children.
Department of Pediatrics, Center of Hematopoietic Stem Cell Transplantation, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, Guangdong Province, China.
Peng-Fei
PF
Wang
Department of Pediatrics, Center of Hematopoietic Stem Cell Transplantation, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, Guangdong Province, China. E-mail: hk01022@sina.com.
Ke
K
Huang
Department of Pediatrics, Center of Hematopoietic Stem Cell Transplantation, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, Guangdong Province, China.
Jian-Pei
JP
Fang
Department of Pediatrics, Center of Hematopoietic Stem Cell Transplantation, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, Guangdong Province, China.
Dun-Hua
DH
Zhou
Department of Pediatrics, Center of Hematopoietic Stem Cell Transplantation, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, Guangdong Province, China.
Hai-Xia
HX
Guo
Children's Hospital of Shanghai, Shanghai 200040, China.
Yan-Yan
YY
Chen
Department of Pediatrics, Center of Hematopoietic Stem Cell Transplantation, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, Guangdong Province, China.
Chun
C
Chen
Department of Pediatrics, Center of Hematopoietic Stem Cell Transplantation, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, Guangdong Province, China.
Yang
Y
Li
D002648:Child; D002675:Child, Preschool; D042241:Early Diagnosis; D005260:Female; D006086:Graft vs Host Disease / Q000175:diagnosis*; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D008297:Male; D055611:Natural Killer T-Cells / Q000166:cytology*; D050378:T-Lymphocytes, Regulatory / Q000166:cytology*; D014184:Transplantation, Homologous