Pilot survey of commercial pasteurized milk consumed in the metropolitan area of Rio de Janeiro, Brazil, for tetracyclines residues, including the 4-epimers of oxytetracycline, tetracycline and chlortetracycline.
This pilot survey aimed to assess the occurrence of tetracyclines and the 4-epimers of oxytetracycline, tetracycline and chlortetracycline in commercial pasteurized milks sold in the metropolitan area of Rio de Janeiro, Brazil, between October 2009 and March 2010. A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method, developed and validated in our laboratory, was used. All 100 analyzed samples were compliant, but 14 contained oxytetracycline in concentrations ranging from the method limit of detection (3.7 µg l(-1)) to the method limit of quantification (12.2 µg l(-1)). One sample contained oxytetracycline and tetracycline simultaneously (at a concentration slightly higher than method limit of quantification, 7.0 µg l(-1)). The presence of 4-epioxytetracycline and 4-epitetracycline in contaminated samples with the parent drugs could not be confirmed as traces were detected only in the quantification MRM transition. No other tetracyclines were detected.
a National Institute for Quality Control in Health/Oswaldo Cruz Foundation (INCQS/FIOCRUZ) , Av. Brasil 4365 , Rio de Janeiro , RJ , Brazil.
Bernardete Ferraz
BF
Spisso
Mychelle Alves
MA
Monteiro
Mararlene Ulberg
MU
Pereira
Rosana Gomes
RG
Ferreira
Rafaela Pinto
RP
da Costa
Tatiana Ávila
TÁ
Cruz
Armi Wanderley
AW
da Nóbrega
D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D001938:Brazil; D002751:Chlortetracycline / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods; D004350:Drug Residues / Q000032:analysis; D005506:Food Contamination / Q000032:analysis; D006801:Humans; D008892:Milk / Q000737:chemistry*; D010118:Oxytetracycline / Q000032:analysis*; D061352:Pasteurization; D053719:Tandem Mass Spectrometry / Q000379:methods; D013752:Tetracycline / Q000032:analysis*; D013754:Tetracyclines / Q000032:analysis*
D000900:Anti-Bacterial Agents; D013754:Tetracyclines; D013752:Tetracycline; D002751:Chlortetracycline; D010118:Oxytetracycline
milk; veterinary drug residues, antibiotics, oxytetracyclin, tetracycline