Design synthesis of polypyrrole-Co3O4 hybrid material for the direct electrochemistry of Hemoglobin and Glucose Oxidase.
We designed and synthesized a novel organic-inorganic hybrid material polypyrrole-Co3O4 (Ppy-Co3O4), then mixed it with ionic liquid (IL) to form stable composite films for the immobilization of Hemoglobin (Hb) and Glucose Oxidase (GOD). The combination of Ppy and Co3O4 as well as IL created a platform with exceptional characteristics, and the content of Ppy had an effect on the direct electron transfer (DET) of Hb/GOD. Notably, when weight percentage of pyrrole monomer was 20%, the heterogenous electron transfer rate constant (ks) for Hb and GOD was estimated to be 1.71s(-1) and 1.67s(-1), respectively. In the meantime, electrochemical and spectroscopic measurements showed that Hb/GOD remained their bioactivity, and achieved fast electron transfer on the Ppy-Co3O4/IL composite film modified electrode. Furthermore, the Ppy-Co3O4/IL/Hb composite film modified electrode was used as a biosensor, and exhibited a long linear range and lower detection limit to H2O2. The apparent Michaelis-Menten constant (Km) was found to be 0.53mM. The sensing design based on the Ppy-Co3O4 hybrid material was demonstrated to be effective and promising in developing protein and enzyme biosensors.
College of Sciences, Xi'an University of Architecture and Technology, Xi'an, 710055, China. Electronic address: dongsyy@126.com.
Sheying
S
Dong
College of Sciences, Xi'an University of Architecture and Technology, Xi'an, 710055, China.
Lei
L
Peng
College of Sciences, Xi'an University of Architecture and Technology, Xi'an, 710055, China.
Dan
D
Liu
College of Sciences, Xi'an University of Architecture and Technology, Xi'an, 710055, China.
Qiangxu
Q
Yang
School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China.
Tinglin
T
Huang
D015374:Biosensing Techniques; D003035:Cobalt / Q000737:chemistry*; D004563:Electrochemistry; D004566:Electrodes; D005949:Glucose Oxidase / Q000737:chemistry*; D006454:Hemoglobins / Q000737:chemistry*; D055767:Immobilized Proteins / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D010087:Oxides / Q000138:chemical synthesis* / Q000737:chemistry; D013499:Surface Properties
D006454:Hemoglobins; D055767:Immobilized Proteins; D010087:Oxides; D003035:Cobalt; D005949:Glucose Oxidase
Direct electron transfer; Hybrid material; Polypyrrole–Co(3)O(4); Protein/enzyme