Urinary Metabolomics on the Biochemical Profiles in Diet-Induced Hyperlipidemia Rat Using Ultraperformance Liquid Chromatography Coupled with Quadrupole Time-of-Flight SYNAPT High-Definition Mass Spectrometry.
Ultraperformance liquid chromatography coupled with quadrupole time-of-flight synapt high-definition mass spectrometry metabolomics was used to characterize the urinary metabolic profiling of diet-induced hyperlipidaemia in a rat model. Analysis was done by orthogonal partial least squares discriminant analysis, correlation analysis, heat map analysis, and KEGG pathways analysis. Potential biomarkers were chosen by S-plot and were identified by accurate mass, isotopic pattern, and MS/MS fragments information. Significant differences in fatty acid, amino acid, nucleoside, and bile acid were observed, indicating the perturbations of fatty acid, amino acid, nucleoside, and bile acid metabolisms in diet-induced hyperlipidaemia rats. This study provides further insight into the metabolic profiling across a wide range of biochemical pathways in response to diet-induced hyperlipidaemia.
Department of Physical Education, Northwest University, No. 229 Taibai North Road, Xi'an, Shaanxi 710069, China.
Hua
H
Miao
Department of Traditional Chinese Medicine, The College of Life Sciences, Northwest University, No. 229 Taibai North Road, Xi'an, Shaanxi 710069, China.
Hua
H
Chen
Department of Physical Education, Northwest University, No. 229 Taibai North Road, Xi'an, Shaanxi 710069, China.
Xu
X
Zhang
Department of Traditional Chinese Medicine, The College of Life Sciences, Northwest University, No. 229 Taibai North Road, Xi'an, Shaanxi 710069, China.
Lu
L
Yin
Department of Traditional Chinese Medicine, The College of Life Sciences, Northwest University, No. 229 Taibai North Road, Xi'an, Shaanxi 710069, China.
Dan-Qian
DQ
Chen
National Institutes for Food and Drug Control, State Food and Drug Administration, 2 Tiantan Xili, Beijing 100050, China.
Xian-Long
XL
Cheng
Solution Centre, Waters Technologies (Shanghai) Ltd., No. 1000 Jinhai Road, Shanghai 201203, China.
Xu
X
Bai
National Institutes for Food and Drug Control, State Food and Drug Administration, 2 Tiantan Xili, Beijing 100050, China.
Feng
F
Wei