Antibody mediated fluorescence enhancement of nucleoside analogue 1,3-diaza-2-oxophenoxazine (tC°).
We report on the generation and analytical application of the monoclonal antibody G93-ED2 raised against the tricyclic fluorescent nucleoside analogue 1,3-diaza-2-oxophenoxazine (tC°). G93-ED2 is specifically binding this deoxycytidine analogue and was found to raise its fluorescence intensity by a factor of 5. This unique feature makes it a valuable tool in fluorescence dependent immunoassays. G93-ED2 was successfully applied in a homogeneous fluorescence quenching immunoassay (DNA-Q) for the sequence specific determination of DNA.
UP Transfer GmbH, Am Neuen Palais 10, Potsdam, Germany. Electronic address: frank.sellrie@up-transfer.de.
Frank
F
Sellrie
UP Transfer GmbH, Am Neuen Palais 10, Potsdam, Germany.
Christine
C
Lenz
UP Transfer GmbH, Am Neuen Palais 10, Potsdam, Germany.
Anika
A
Andersson
Department of Chemical and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden.
L Marcus
LM
Wilhelmsson
UP Transfer GmbH, Am Neuen Palais 10, Potsdam, Germany; Hybrotec GmbH, Am Mühlenberg 11, Potsdam, Germany.
Jörg A
JA
Schenk
D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000276:immunology; D000915:Antibody Affinity / Q000276:immunology; D000918:Antibody Specificity / Q000276:immunology; D004247:DNA / Q000737:chemistry / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005453:Fluorescence; D007118:Immunoassay / Q000379:methods*; D015394:Molecular Structure; D010078:Oxazines / Q000737:chemistry* / Q000276:immunology; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence
C526502:1,3-diaza-2-oxophenoxazine; D000911:Antibodies, Monoclonal; D010078:Oxazines; D004247:DNA
1,3-Diaza-2-oxophenoxazine (tC°); Fluorescence enhancement; Fluorescent nucleoside analogue; Homogeneous immunoassay; Hypsochromic shift; Monoclonal antibody