[Expression analyses of BcUGT3 and BcUGT6, and their in vitro expression in Escherichia coli].
The tissue-specific and MeJA-induced transcriptional levels of BcUGT3 and BcUGT6 in Bupleurum chinense were analyzed in the present study. The transcriptional levels of BcUGT3 in root, leaf, flower and fruit were similar and they all were higher than those in stem. The transcriptional level of BcUGT6 was the highest in leaf and the lowest in flower among in all tested tissues. With non-treated adventitious roots as control, BcUGT6's transcriptional levels were elevated to nearly 2 folds for 2 h, 8 h, 24 h, 2 d and 4 d in MeJA-treated adventitious roots of B. chinense. It showed that the transcriptional level of BcUGT6 was slightly affected by MeJA. While, BcUGT3's transcriptional levels were gradually elevated, and till 4 d after MeJA treatment, the expression level was about 7 folds than that of non-treated control. Using pET-28a (+), the expressions of two genes was investigated. Induced by IPTG, the target proteins were expressed in E. coli and then purified. All the results obtained in the present study will be helpful for follow-up bio-function analysis of BcUGT3 and BcUGT6.
Yun-Wen
YW
Tao
Jie-Sen
JS
Xu
Jing
J
Sun
Jian-He
JH
Wei
Juan
J
Liu
Chun
C
Sui
D000085:Acetates / Q000494:pharmacology; D029972:Bupleurum / Q000166:cytology / Q000201:enzymology* / Q000235:genetics*; D002462:Cell Membrane / Q000378:metabolism; D003517:Cyclopentanes / Q000494:pharmacology; D004926:Escherichia coli / Q000235:genetics*; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D006602:Hexosyltransferases / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D042541:Intracellular Space / Q000378:metabolism; D054883:Oxylipins / Q000494:pharmacology; D021382:Protein Sorting Signals; D017433:Protein Structure, Secondary; D021381:Protein Transport; D017421:Sequence Analysis; D014158:Transcription, Genetic / Q000187:drug effects
D000085:Acetates; D003517:Cyclopentanes; D054883:Oxylipins; D021382:Protein Sorting Signals; C072239:methyl jasmonate; D006602:Hexosyltransferases