Analysing the effects of frozen storage and processing on the metabolite profile of raw mullet roes using ¹H NMR spectroscopy.
(1)H NMR spectroscopy was used to investigate changes in the low molecular weight metabolic profile of raw mullet (Mugil spp.) roes during frozen storage and upon processing. NMR data were analysed by Principal Component Analyses (PCA). In the model constructed using frozen roes, no statistical significant metabolic modifications were observed in the first six months of storage, while choline derivatives, dimethylamine, lactate, and most of the free amino acids were identified as changing with statistical significance (p<0.05) in response to frozen storage time of twelve months. The PCA model comparing the metabolic profiles of roes before and after processing showed that the major modifications occurring upon manufacturing were the increase of the choline derivative compounds, uracil, and free amino acids, and a large decrease of taurine, glucose, lactate, and creatine/phosphocreatine. All of the above mentioned modifications reflect the occurrence of chemical/biochemical reactions arising from degradation processes such as lipolysis and proteolysis.
Dipartimento di Scienze Chimiche e Geologiche, Università degli Studi di Cagliari, Cittadella di Monserrato, SS 554, 09042 Monserrato, CA, Italy.
Cristina
C
Piras
Dipartimento di Scienze Chimiche e Geologiche, Università degli Studi di Cagliari, Cittadella di Monserrato, SS 554, 09042 Monserrato, CA, Italy.
Paola
P
Scano
Dipartimento di Scienze Chimiche e Geologiche, Università degli Studi di Cagliari, Cittadella di Monserrato, SS 554, 09042 Monserrato, CA, Italy.
Emanuela
E
Locci
Dipartimento di Scienze Chimiche e Geologiche, Università degli Studi di Cagliari, Cittadella di Monserrato, SS 554, 09042 Monserrato, CA, Italy.
Roberta
R
Sanna
Dipartimento di Scienze Chimiche e Geologiche, Università degli Studi di Cagliari, Cittadella di Monserrato, SS 554, 09042 Monserrato, CA, Italy. Electronic address: flaminia@unica.it.
Flaminia Cesare
FC
Marincola
D000818:Animals; D005396:Fish Products / Q000032:analysis; D005511:Food Handling; D061353:Food Storage; D005615:Freezing; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D025341:Principal Component Analysis; D023701:Smegmamorpha / Q000378:metabolism*
(1)H NMR; Frozen storage; Metabolomics; Mullet roes; Processing