Susceptibility of nine organophosphorus pesticides in skimmed milk towards inoculated lactic acid bacteria and yogurt starters.
BACKGROUND
Previous research has shown that fresh milk might be polluted by some organophosphorus pesticides (OPPs). In this study the dissipation of nine OPPs, namely chlorpyrifos, chlorpyrifos-methyl, diazinon, dichlorvos, fenthion, malathion, phorate, pirimiphos-methyl and trichlorphon, in skimmed milk was investigated to clarify their susceptibility towards lactic acid bacteria (LAB) and yogurt starters.


RESULTS
Skimmed milk was spiked with nine OPPs, inoculated with five strains of LAB and two commercial yogurt starters at 42 °C for 24 and 5 h respectively and subjected to quantitative OPP analysis by gas chromatography. Degradation kinetic constants of these OPPs were calculated based on a first-order reaction model. OPP dissipation in the milk was enhanced by the inoculated strains and starters, resulting in OPP concentrations decreasing by 7.0-64.6 and 7.4-19.2% respectively. Totally, the nine OPPs were more susceptible to Lactobacillus bulgaricus, as it enhanced their degradation rate constants by 18.3-133.3%. Higher phosphatase production of the assayed stains was observed to bring about greater OPP degradation in the milk.


CONCLUSIONS
Both LAB and yogurt starters could enhance OPP dissipation in skimmed milk, with the nine OPPs studied having different susceptibilities towards them. Phosphatase was a key factor governing OPP dissipation. The LAB of higher phosphatase production have more potential to decrease OPPs in fermented foods.
Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin, 150030, China.
Xin-Wei
XW
Zhou
Xin-Huai
XH
Zhao
D000818:Animals; D005506:Food Contamination / Q000032:analysis / Q000517:prevention & control*; D006801:Humans; D007778:Lactobacillus / Q000201:enzymology / Q000378:metabolism*; D008892:Milk / Q000737:chemistry / Q000382:microbiology; D010755:Organophosphates / Q000032:analysis; D010575:Pesticides / Q000032:analysis; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D015014:Yogurt / Q000382:microbiology*
D010755:Organophosphates; D010575:Pesticides; D010744:Phosphoric Monoester Hydrolases
lactic acid bacteria; organophosphorus pesticides; phosphatase; skimmed milk; susceptibility; yogurt starter