[Analysis of monosaccharide compositions in polysaccharides from exopleura of Ginkgo biloba].
A high-performance liquid chromatography method of pre-column derivatization with 1-phenyl-3-methyl-5 -pyrazolone (PMP) has been established for determination of 6 kinds of monosaccharides simultaneously. A special Agilent HC-C18 column (4. 6 mm x 250 mm, 5 microm), optimized for the separation of PMP derivatives, was used at ambient temperature of 40 degrees C. The PMP derivatives elution was performed with a mixture of 0.1 mol x L(-1) phosphate buffer (pH 6. 8) and acetonitrile in a ratio of 84: 16 at a flow rate of 1 mL x min(-1), and UV absorbance of the effluent was monitored at 245 nm. The results showed that the polysaccharides from exopleura of Ginkgo biloba were acidic heteropolysaccharides mainly containing mannose, rhamnose, D-galacturonic acid, glucose, galactose, arabinose, with the molar ratio of 0.032: 0.14: 0.296: 0.403:0.106: 0.046.
Lei-Lei
LL
Mao
Ying
Y
Chen
Bi-Yuan
BY
Hu
Ai-Hua
AH
Xu
D020441:Ginkgo biloba / Q000737:chemistry*; D006868:Hydrolysis; D009005:Monosaccharides / Q000032:analysis*; D035261:Plant Components, Aerial / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry*
D009005:Monosaccharides; D011134:Polysaccharides