Sensitive quantitation of polyamines in plant foods by ultrasound-assisted benzoylation and dispersive liquid-liquid microextraction with the aid of experimental designs.
A new method involving ultrasound-assisted benzoylation and dispersive liquid-liquid microextraction was optimized with the aid of chemometrics for the extraction, cleanup, and determination of polyamines in plant foods. Putrescine, cadaverine, spermidine, and spermine were derivatized with 3,5-dinitrobenzoyl chloride and extracted by dispersive liquid-liquid microextraction using acetonitrile and carbon tetrachloride as dispersive and extraction solvents, respectively. Two-level full factorial design and central composite design were applied to select the most appropriate derivatization and extraction conditions. The developed method was linear in the 0.5-10.0 mg/L range, with a R(2) ≥ 0.9989. Intra- and interday precisions ranged from 0.8 to 6.9% and from 3.0 to 10.3%, respectively, and the limit of detection ranged between 0.018 and 0.042 μg/g of fresh weight. This method was applied to the analyses of six different types of plant foods, presenting recoveries between 81.7 and 114.2%. The method is inexpensive, versatile, simple, and sensitive.
REQUIMTE/Departamento de Ciências Químicas, Laboratório de Bromatologia e Hidrologia, Faculdade de Farmácia, Universidade do Porto . Rua de Jorge Viterbo Ferreira 228, 4050-313 Porto, Portugal.
Edgar
E
Pinto
Armindo
A
Melo
Isabel M P L V O
IM
Ferreira
D002851:Chromatography, High Pressure Liquid; D005638:Fruit / Q000737:chemistry*; D059627:Liquid Phase Microextraction / Q000295:instrumentation / Q000379:methods*; D010936:Plant Extracts / Q000032:analysis* / Q000302:isolation & purification*; D011073:Polyamines / Q000032:analysis* / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D014465:Ultrasonics / Q000379:methods*; D014675:Vegetables / Q000737:chemistry*
D010936:Plant Extracts; D011073:Polyamines