MicroRNA-410 reduces the expression of vascular endothelial growth factor and inhibits oxygen-induced retinal neovascularization.
Retinal neovascularization (RNV) is an eye disease that can cause retinal detachment and even lead to blindness. RNV mainly occurs in the elderly population. The pathogenesis of RNV has been previously reported to be highly related to the expression of vascular endothelial growth factor A (VEGFA), basic fibroblast growth factor (bFGF) and other angiogenic factors. It has also been reported that VEGFA and other factors associated with RNV could be regulated by certain microRNAs (miRNA), a group of small non-coding RNAs which are able to regulate the expression of many genes in vivo. Here, we demonstrate that the miRNA miR-410 is highly expressed in mice within two weeks after birth. miR-410 could suppress VEGFA expression through interaction with the 3'UTR of the VEGFA messenger RNA. Overexpressing a miR-410 mimic effectively suppresses VEGFA expression in various cell lines. Further experiments on oxygen-induced retinopathy (OIR) in mice revealed that eye drops containing large amounts of miR-410 efficiently downregulate VEGFA expression, prevent retinal angiogenesis and effectively treat RNV. These results not only show the underlying mechanism of how miR-410 targets VEGFA but also provide a potential treatment strategy for RNV that might be used in the near future.
Department of Ophthalmology, Ren Ji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China; Department of Ophthalmology, First Affiliated Hospital of Second Military Medical University, Shanghai, China.
Na
N
Chen
Clinical Research Center, Changhai Hospital, Second Military Medical University, Shanghai, China.
Jiaqi
J
Wang
Clinical Research Center, Changhai Hospital, Second Military Medical University, Shanghai, China.
Yijun
Y
Hu
Department of Sea-Air Examination Center, Navy General Hospital, Beijing, China.
Bei
B
Cui
Department of Laboratory Diagnosis, First Affiliated Hospital of Second Military Medical University, Shanghai, China.
Wenjie
W
Li
Clinical Research Center, Changhai Hospital, Second Military Medical University, Shanghai, China.
Guixia
G
Xu
Department of Ophthalmology, Ren Ji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Lin
L
Liu
Clinical Research Center, Changhai Hospital, Second Military Medical University, Shanghai, China.
Shanrong
S
Liu
D020413:3' Untranslated Regions / Q000235:genetics; D000818:Animals; D005786:Gene Expression Regulation / Q000187:drug effects / Q000235:genetics*; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D010100:Oxygen / Q000494:pharmacology*; D015861:Retinal Neovascularization / Q000139:chemically induced* / Q000235:genetics*; D015854:Up-Regulation / Q000187:drug effects / Q000235:genetics; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics*
D020413:3' Untranslated Regions; C568527:MIRN410 microRNA, human; D035683:MicroRNAs; D042461:Vascular Endothelial Growth Factor A; D010100:Oxygen