Distribution of cortical granules and meiotic maturation of canine oocytes in bi-phasic systems.
The aim of this study was to evaluate the influence of different bi-phasic systems with gonadotrophins and steroids on in vitro maturation rates of oocytes obtained from bitches at different reproductive stages (follicular, luteal, anoestrous). In System A (control) oocytes were matured for 72h in base medium (BM) with 10IUmL(-1) human chorionic gonadotrophin (hCG), 1μgmL(-1) progesterone (P4) and 1μgmL(-1) oestradiol (E2); in bi-phasic System B oocytes were matured for 48h in BM with hCG and for 24h in BM with P4; in bi-phasic System C oocytes were matured for 48h in BM with hCG, P4 and E2, and for 24h in BM with P4; in System D, oocytes were cultured in BM without hormonal supplementation. Data were analysed by ANOVA. There was a positive effect of the bi-phasic systems on germinal vesicle breakdown, metaphase I and metaphase II rates, irrespective of reproductive status (P<0.05). Bi-phasic systems were also beneficial for cortical granule distribution (an indication of cytoplasmic maturation) and its relationship to nuclear status: 74.5% of the oocytes cultured in System B and 85.4% of those cultured in System C presented both nuclear and cytoplasmic maturation (P<0.001). The stage of the oestrous cycle did not influence maturation rates.
UNESP - Univ Estadual Paulista, Jaboticabal Campus, Departamento de Medicina Veterinária Preventiva e Reprodução Animal, Via de Acesso Prof. Paulo Donato Castellane, s/n, 14884-900, Jaboticabal-SP, Brazil.
Maricy
M
Apparicio
UNESP - Univ Estadual Paulista, Jaboticabal Campus, Departamento de Medicina Veterinária Preventiva e Reprodução Animal, Via de Acesso Prof. Paulo Donato Castellane, s/n, 14884-900, Jaboticabal-SP, Brazil.
Giuliano Q
GQ
Mostachio
UNESP - Univ Estadual Paulista, Jaboticabal Campus, Departamento de Medicina Veterinária Preventiva e Reprodução Animal, Via de Acesso Prof. Paulo Donato Castellane, s/n, 14884-900, Jaboticabal-SP, Brazil.
Tathiana F
TF
Motheo
UNESP - Univ Estadual Paulista, Jaboticabal Campus, Departamento de Medicina Veterinária Preventiva e Reprodução Animal, Via de Acesso Prof. Paulo Donato Castellane, s/n, 14884-900, Jaboticabal-SP, Brazil.
Aracelle E
AE
Alves
UNESP - Univ Estadual Paulista, Jaboticabal Campus, Departamento de Medicina Veterinária Preventiva e Reprodução Animal, Via de Acesso Prof. Paulo Donato Castellane, s/n, 14884-900, Jaboticabal-SP, Brazil.
Luciana
L
Padilha
UNESP - Univ Estadual Paulista, Jaboticabal Campus, Departamento de Medicina Veterinária Preventiva e Reprodução Animal, Via de Acesso Prof. Paulo Donato Castellane, s/n, 14884-900, Jaboticabal-SP, Brazil.
Eliandra A
EA
Pires-Butler
UNESP - Univ Estadual Paulista, Jaboticabal Campus, Departamento de Medicina Veterinária Preventiva e Reprodução Animal, Via de Acesso Prof. Paulo Donato Castellane, s/n, 14884-900, Jaboticabal-SP, Brazil.
Paula A P
PA
Savi
UNESP - Univ Estadual Paulista, Jaboticabal Campus, Departamento de Medicina Veterinária Preventiva e Reprodução Animal, Via de Acesso Prof. Paulo Donato Castellane, s/n, 14884-900, Jaboticabal-SP, Brazil.
Ricardo A R
RA
Uscategui
Dipartimento di Scienze Veterinarie per la Salute, la Produzione Animale e la Sicurezza Alimentare, Università degli Studi di Milano, Via Celoria 10, 20133 Milano, Italy.
Gaia C
GC
Luvoni
UNESP - Univ Estadual Paulista, Jaboticabal Campus, Departamento de Medicina Veterinária Preventiva e Reprodução Animal, Via de Acesso Prof. Paulo Donato Castellane, s/n, 14884-900, Jaboticabal-SP, Brazil.
Wilter R R
WR
Vicente
D000818:Animals; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D004285:Dogs; D004958:Estradiol / Q000494:pharmacology; D030762:Estrous Cycle; D005260:Female; D008540:Meiosis / Q000187:drug effects* / Q000502:physiology; D009865:Oocytes / Q000187:drug effects / Q000254:growth & development*; D011374:Progesterone / Q000494:pharmacology
D006063:Chorionic Gonadotropin; D011374:Progesterone; D004958:Estradiol