Changes in plasma intact parathyroid hormone levels following 24-hour incubation at room temperature as determined by Roche electrochemiluminescence PTH immunoassay.
BACKGROUND
Estimation of circulatory PTH is necessary for correct diagnosis of diseases related to the parathyroid gland, bone, and kidney.


METHODS
Roche Electrochemiluminescence PTH immunoassay was used to measure changes in plasma iPTH concentration in 100 random samples following a 24 hours incubation at room temperature.


RESULTS
Low to normal iPTH concentration individuals showed higher decrease in iPTH concentration as compared to individuals with elevated PTH and the rate of PTH degradation was significantly higher (p < 0.05) in the low to normal PTH group.


CONCLUSIONS
Blood samples from suspected hypo- or normo-parathyroid subjects should be analyzed as soon as possible as a little degradation of PTH at room temperature could influence their clinical interpretation.
Ashutosh Kumar
AK
Arya
Naresh
N
Sachdeva
D006801:Humans; D007118:Immunoassay; D010281:Parathyroid Hormone / Q000097:blood*; D013048:Specimen Handling / Q000009:adverse effects; D013696:Temperature
D010281:Parathyroid Hormone