Ratiometric near-infrared chemosensor for trivalent chromium ion based on tricarboyanine in living cells.
A tricarboyanine derivative (IRPP) is applied as a ratiometric near-infrared chemosensor for detecting trivalent chromium ions (Cr(3+)) in living cells. Upon the addition of Cr(3+) to a solution of IRPP, large-scale shifts in the emission spectrum (from 755 nm to 561 nm) are observed. In the newly developed sensing system, these well-resolved emission peaks yield a sensing system that covers a linear range from 1.0×10(-7) to 1.0×10(-5) M with a detection limit of 2.5×10(-8) M. The experimental results show the response behavior of IRPP towards Cr(3+) is pH independent under neutral conditions (6.0-7.5). Most importantly, the fast response time (less than 3 min) and selectivity for Cr(3+) over other common metal ions provide a strong argument for the use of this sensor in real world applications. As a proof of concept, the proposed chemosensor has been used to detect and quantify Cr(3+) in river water samples and to image Cr(3+) in living cells with encouraging results.
Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan 411105, PR China. Electronic address: lichunyan79@sina.com.
Chun-Yan
CY
Li
Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan 411105, PR China.
Xue-Fei
XF
Kong
College of Chemical Engineering, Xiangtan University, Xiangtan 411105, PR China.
Yong-Fei
YF
Li
Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan 411105, PR China.
Chao
C
Weng
Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan 411105, PR China.
Jia-Liang
JL
Tang
Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan 411105, PR China. Electronic address: zwg18@xtu.edu.cn.
Dan
D
Liu
Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan 411105, PR China.
Wei-Guo
WG
Zhu
D002470:Cell Survival; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation*; D002857:Chromium / Q000032:analysis* / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007259:Infrared Rays; D011725:Pyridines / Q000737:chemistry*
D005456:Fluorescent Dyes; C000596852:IRPP compound; D011725:Pyridines; D002857:Chromium
Chemosensor; Chromium ion; Near-infrared; Ratiometric; Tricarboyanine