Exhaled and plasma nitrite: a comparative study among healthy, cirrhotic and liver transplant patients.
BACKGROUND
There is a relative lack of studies about exhaled nitrite (NO2-) concentrations in cirrhotic and transplanted patients.


OBJECTIVE
Verify possible differences and correlations between the levels of NO2-, measured in plasma and exhaled breath condensate collected from patients with cirrhosis and liver transplant.


METHODS
Sixty adult male patients, aged between 27 and 67 years, were subdivided into three groups: a control group comprised of 15 healthy volunteers, a cirrhosis group composed of 15 volunteers, and a transplant group comprised of 30 volunteers. The NO2- concentrations were measured by chemiluminescence.


RESULTS
1) The analysis of plasma NO2- held among the three groups showed no statistical significance. 2) The comparison between cirrhotic and control groups, control and transplanted and cirrhotic and transplanted was not statistically significant. 3) The measurements performed on of NO2- exhaled breath condensate among the three groups showed no statistical difference. 4) When comparing the control group samples and cirrhotic, control and transplanted and cirrhotic and transplanted, there was no significant changes in the concentrations of NO2-.


CONCLUSIONS
No correlations were found between plasma and exhaled NO2-, suggesting that the exhaled NO2- is more reflective of local respiratory NO release than the systemic circulation.
Faculdade de Medicina de Ribeirão Preto, Departamento de Cirurgia e Anatomia, Universidade de São Paulo, São Paulo, Brasil, Brasil.
Viviane S
VS
Augusto
Faculdade de Medicina de Ribeirão Preto, Departamento de Cirurgia e Anatomia, Universidade de São Paulo, São Paulo, Brasil, Brasil.
Alfredo J
AJ
Rodrigues
Faculdade de Medicina de Ribeirão Preto, Departamento de Cirurgia e Anatomia, Universidade de São Paulo, São Paulo, Brasil, Brasil.
Ana Paula
AP
C Silveira
Faculdade de Medicina de Ribeirão Preto, Departamento de Cirurgia e Anatomia, Universidade de São Paulo, São Paulo, Brasil, Brasil.
Orlando
O
de Castro E Silva
Faculdade de Medicina de Ribeirão Preto, Departamento de Cirurgia e Anatomia, Universidade de São Paulo, São Paulo, Brasil, Brasil.
Enio David
ED
Mente
Faculdade de Medicina de Ribeirão Preto, Departamento de Cirurgia e Anatomia, Universidade de São Paulo, São Paulo, Brasil, Brasil.
Paulo Roberto B
PR
Evora
D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000032:analysis / Q000097:blood; D001944:Breath Tests / Q000379:methods*; D016022:Case-Control Studies; D045853:Exhalation; D006801:Humans; D008103:Liver Cirrhosis / Q000378:metabolism*; D016031:Liver Transplantation; D008297:Male; D008875:Middle Aged; D009569:Nitric Oxide / Q000378:metabolism*; D009573:Nitrites / Q000032:analysis* / Q000097:blood
D015415:Biomarkers; D009573:Nitrites; D009569:Nitric Oxide