Association mapping of yield-related traits and SSR markers in wild soybean (Glycine soja Sieb. and Zucc.).
Wild soybean, the progenitor of cultivated soybean, is an important gene pool for ongoing soybean breeding efforts. To identify yield-enhancing quantitative trait locus (QTL) or gene from wild soybean, 113 wild soybeans accessions were phenotyped for five yield-related traits and genotyped with 85 simple sequence repeat (SSR) markers to conduct association mapping. A total of 892 alleles were detected for the 85 SSR markers, with an average 10.49 alleles; the corresponding PIC values ranged from 0.07 to 0.92, with an average 0.73. The genetic diversity of each SSR marker ranged from 0.07 to 0.93, with an average 0.75. A total of 18 SSR markers were identified for the five traits. Two SSR markers, sct_010 and satt316, which are associated with the yield per plant were stably expressed over two years at two experimental locations. Our results suggested that association mapping can be an effective approach for identifying QTL from wild soybean.
National Key Laboratory of Crop Genetics and Germplasm Enhancement, National Center for Soybean Improvement, Nanjing Agricultural University , Nanjing 210095 , China ; Henan Center of Crop Design, Henan Academy of Agricultural Science , Zhengzhou 450002 , China.
Zhenbin
Z
Hu
National Key Laboratory of Crop Genetics and Germplasm Enhancement, National Center for Soybean Improvement, Nanjing Agricultural University , Nanjing 210095 , China ; Department of Agronomy, Henan Agricultural University , Zhengzhou 450002 , China.
Dan
D
Zhang
National Key Laboratory of Crop Genetics and Germplasm Enhancement, National Center for Soybean Improvement, Nanjing Agricultural University , Nanjing 210095 , China.
Guozheng
G
Zhang
National Key Laboratory of Crop Genetics and Germplasm Enhancement, National Center for Soybean Improvement, Nanjing Agricultural University , Nanjing 210095 , China.
Guizhen
G
Kan
National Key Laboratory of Crop Genetics and Germplasm Enhancement, National Center for Soybean Improvement, Nanjing Agricultural University , Nanjing 210095 , China.
Delin
D
Hong
National Key Laboratory of Crop Genetics and Germplasm Enhancement, National Center for Soybean Improvement, Nanjing Agricultural University , Nanjing 210095 , China.
Deyue
D
Yu
association mapping; genetic diversity; population structure; wild soybean; yield-related traits