DNA nanosensor based on biocompatible graphene quantum dots and carbon nanotubes.
An ultrasensitive nanosensor based on fluorescence resonance energy transfer (FRET) between biocompatible graphene quantum dots and carbon nanotubes for DNA detection was reported. We take advantage of good biocompatibility and strong fluorescence of graphene quantum dots, base pairing specificity of DNA and unique fluorescence resonance energy transfer between graphene quantum dots and carbon nanotubes to achieve the analysis of low concentrations of DNA. Graphene quantum dots with high quantum yield up to 0.20 were prepared and served as the fluorophore of DNA probe. FRET process between graphene quantum dots-labeled probe and oxidized carbon nanotubes is easily achieved due to their efficient self-assembly through specific π-π interaction. This nanosensor can distinguish complementary and mismatched nucleic acid sequences with high sensitivity and good reproducibility. The detection method based on this nanosensor possesses a broad linear span of up to 133.0 nM and ultralow detection limit of 0.4 nM. The constructed nanosensor is expected to be highly biocompatible because of all its components with excellent biocompatibility.
College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China.
Zhao Sheng
ZS
Qian
College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China.
Xiao Yue
XY
Shan
College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China.
Lu Jing
LJ
Chai
College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China.
Juan Juan
JJ
Ma
College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China.
Jian Rong
JR
Chen
College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China. Electronic address: fenghui@zjnu.cn.
Hui
H
Feng
D001672:Biocompatible Materials / Q000138:chemical synthesis; D015374:Biosensing Techniques / Q000295:instrumentation*; D004247:DNA / Q000032:analysis / Q000235:genetics*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D031541:Fluorescence Resonance Energy Transfer / Q000295:instrumentation*; D006108:Graphite / Q000737:chemistry*; D036103:Nanotechnology / Q000295:instrumentation; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation; D045663:Quantum Dots; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000295:instrumentation*
D001672:Biocompatible Materials; D037742:Nanotubes, Carbon; D006108:Graphite; D004247:DNA
Carbon nanotubes; DNA detection; Graphene quantum dots; Nanosensor