Bioinformatical evaluation of desiccation-responsive rd29A gene in Arabidopsis thaliana.
In Arabidopsis thaliana expression of two Desiccation-Responsive rd29 genes are intensity induced under drought, salinity and temperature (heat and cold) conditions. These two genes called, rd29A and rd29B are located in 8-9 Kb of Arabidopsis genome. Also, transgenic plants containing these genes have more tolerant toward environmental stresses. Since rd29A has an important role in various non-biological stresses, in this study genetic and promoter analysis of this gene was performed, rd29A nucleotide sequence was obtained from NCBI gene bank, for bioinformatic analysis used the Map viewer, Plant care and Genevestigator software. In Arabidopsis rd29A has 8048 nucleotides and encode a linear mRNA with 2133bp. It located at AT5G52310 locus on chromosome 5 between nucleotides 21241898 and 21242457 and contains 4 exons and 3 introns. Promoter region analysis to determine the gene regulatory elements performed with using 1500 bp of the 5 'UTR sequence and the results indicated that the existence of diverse regulatory elements such as ABRE, DRE, Box I, C-repeat/DRE and TCA-element which represent involvement of this gene in different physiological pathways in plants. In addition the TATA-Box was identified as Core promoter in -30 position. Also, the pattern of gene expression was examined by Microarrays data and it was found that the expression levels were variable in different developmental stages, so that the lowest and highest expressions were in germination and mature stages. It seems that using of this gene promoter in expression cassettes can raise tolerance of transgenic plants to environmental stresses.
Mostafa
M
Modarresi
Alireza
A
Eyvazi
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics* / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics*; D019295:Computational Biology; D055864:Droughts; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D010802:Phylogeny; D013312:Stress, Physiological / Q000235:genetics
D029681:Arabidopsis Proteins; C520629:RD29a protein, Arabidopsis