Effect of N-arachidonoyl dopamine on activity of neuronal network in primary hippocampus culture upon hypoxia modelling.
We studied the effect of endocannabinoid N-arachidonoyl dopamine on spontaneous bioelectric activity of cultured hippocampal neurons in a model of hypoxia/reoxygenation. Incubation under hypoxic conditions induced irreversible decrease in spontaneous bioelectric activity of neurons and their death. Application of N-arachidonoyl dopamine during hypoxia and in the post-hypoxic period preserved bioelectric activity and viability of neurons. The protective effect of N-arachidonoyl dopamine was primarily mediated by type I cannabinoid receptors.
Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russia, MVedunova@yandex.ru.
M V
MV
Vedunova
E V
EV
Mitroshina
T A
TA
Sakharnova
M Yu
MY
Bobrov
V V
VV
Bezuglov
L G
LG
Khaspekov
I V
IV
Mukhina
D000200:Action Potentials; D000818:Animals; D001095:Arachidonic Acids / Q000494:pharmacology*; D015687:Cell Hypoxia; D002478:Cells, Cultured; D004298:Dopamine / Q000031:analogs & derivatives* / Q000494:pharmacology; D004353:Drug Evaluation, Preclinical; D006624:Hippocampus / Q000166:cytology*; D051379:Mice; D009415:Nerve Net / Q000187:drug effects / Q000502:physiology; D009474:Neurons / Q000502:physiology; D018696:Neuroprotective Agents / Q000494:pharmacology*; D061251:Primary Cell Culture
D001095:Arachidonic Acids; D018696:Neuroprotective Agents; C474941:arachidonyl dopamine; D004298:Dopamine