Improving methane production and phosphorus release in anaerobic digestion of particulate saline sludge from a brackish aquaculture recirculation system.
In this study, batch tests were conducted to examine the effects of trehalose and glycine betaine as well as potassium on the specific methanogenic activity (SMA), acid and alkaline phosphatase activity of anaerobic biomass and phosphorus release in anaerobic digestion of saline sludge from a brackish recirculation aquaculture system. The results of ANOVA and Tukey's HSD (honestly significant difference) tests showed that glycine betaine and trehalose enhanced SMA of anaerobic biomass and reactive phosphorus release from the particulate waste. Moreover, SMA tests revealed that methanogenic sludge, which was long-term acclimatized to a salinity level of 17 g/L was severely affected by the increase in salinity to values exceeding 35 g/L. Addition of compatible solutes, such as glycine betaine and trehalose, could be used to enhance the specific methane production rate and phosphorus release in anaerobic digestion from particulate organic waste produced in marine or brackish aquaculture recirculation systems.
Faculty of Civil Engineering and Geosciences, Department of Water Management, Sanitary Engineering Section, Delft University of Technology, Stevinweg 1, 2628 CN Delft, The Netherlands. Electronic address: x.zhang@tudelft.nl.
Xuedong
X
Zhang
Faculty of Engineering of University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal.
Rui B
RB
Ferreira
Faculty of Civil Engineering and Geosciences, Department of Water Management, Sanitary Engineering Section, Delft University of Technology, Stevinweg 1, 2628 CN Delft, The Netherlands.
Jianmei
J
Hu
Faculty of Civil Engineering and Geosciences, Department of Water Management, Sanitary Engineering Section, Delft University of Technology, Stevinweg 1, 2628 CN Delft, The Netherlands.
Henri
H
Spanjers
Faculty of Civil Engineering and Geosciences, Department of Water Management, Sanitary Engineering Section, Delft University of Technology, Stevinweg 1, 2628 CN Delft, The Netherlands.
Jules B
JB
van Lier
D000693:Anaerobiosis / Q000187:drug effects; D017756:Aquaculture; D001622:Betaine / Q000494:pharmacology; D018533:Biomass; D008697:Methane / Q000096:biosynthesis*; D052638:Particulate Matter / Q000302:isolation & purification*; D010749:Phosphoprotein Phosphatases / Q000378:metabolism; D010758:Phosphorus / Q000302:isolation & purification*; D011188:Potassium / Q000494:pharmacology; D054712:Salinity; D012722:Sewage / Q000737:chemistry*; D012965:Sodium Chloride / Q000494:pharmacology; D014199:Trehalose / Q000494:pharmacology; D018508:Water Purification / Q000379:methods*
D052638:Particulate Matter; D012722:Sewage; D010758:Phosphorus; D001622:Betaine; D012965:Sodium Chloride; D014199:Trehalose; D010749:Phosphoprotein Phosphatases; D008697:Methane; D011188:Potassium
Acid/alkaline phosphatase activity; Brackish aquaculture system; Compatible solutes; Phosphorus release; Specific methanogenic activity