Fast separation and determination of free myo-inositol by hydrophilic liquid chromatography.
A fast liquid chromatography method for separation and determination of myo-inositol is reported. Determination of the biologically important isomer of inositols, myo-inositol, was optimized to avoid overlapping to possible interferents according to European Pharmacopoeia (glycerol, d-mannitol) and saccharose. The method in HILIC mode is extremely selective to other carbohydrates which allows to separate myo-inositol from allo- and d-chiro-inositol with resolution 12.3 and 5.2, resp. and this way it enables to separate myo-inostiol from contingent carbohydrates present in a sample matrix. Retention time of myo-inositol was 12min at 10°C, though higher temperatures (25°C or 40°C) or higher water content in the mobile phase could speed up the separation and determination to four minutes. LOD of the method was 9mg/L at 10°C, and 5mg/L at 25°C, resp.
Department of Chemical Drugs, University of Veterinary and Pharmaceutical Science, Palackého 1/3, CZ-611 42 Brno, Czech Republic. Electronic address: pazourekj@vfu.cz.
Jiří
J
Pazourek
D002851:Chromatography, High Pressure Liquid; D057927:Hydrophobic and Hydrophilic Interactions; D007294:Inositol / Q000031:analogs & derivatives / Q000032:analysis* / Q000737:chemistry*; D013997:Time Factors
D007294:Inositol
ELSD; HILIC; myo-inositol