Efficient gene carriers composed of 2-hydroxypropyl-β-cyclodextrin, ethanolamine-functionalized poly(glycidyl methacrylate), and poly((2-dimethyl amino)ethyl methacrylate) by combination of ATRP and click chemistry.
In this work, a simple one-step method is first employed to produce the bromoisobutyryl-terminated 2-hydroxypropyl-β-cyclodextrin (HPCD-Br). The pendant epoxy groups of poly(glycidyl methacrylate) block prepared via ATRP from HPCD-Br can be reacted with ethanolamine to produce HPCD-PGEA which exhibits much lower cytotoxicity and better gene transfection yield than polyethylenimine (25 kDa) in COS7 and HepG2 cell lines. Moreover, poly((2-dimethyl amino)ethyl methacrylate) blocks can be incorporated into low-molecular-weight HPCD-PGEA via "click" reaction to further enhance the gene transfection efficiency in HepG2 cell lines.
State Key Laboratory of Chemical Resource Engineering, Key Laboratory of Carbon Fiber and Functional Polymers, Ministry of Education, Beijing University of Chemical Technology, Beijing, 100029, China.
Yun
Y
Zhu
Xuefeng
X
Zheng
Bingran
B
Yu
Wantai
W
Yang
Nana
N
Zhao
Fujian
F
Xu
D000073738:2-Hydroxypropyl-beta-cyclodextrin; D000818:Animals; D019556:COS Cells; D002470:Cell Survival; D002522:Chlorocebus aethiops; D057930:Click Chemistry / Q000379:methods*; D024202:Electrophoretic Mobility Shift Assay; D019856:Ethanolamine / Q000737:chemistry*; D005822:Genetic Vectors / Q000235:genetics*; D056945:Hep G2 Cells; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008689:Methacrylates / Q000737:chemistry*; D009757:Nylons / Q000737:chemistry*; D010957:Plasmids / Q000235:genetics; D058105:Polymerization; D011109:Polymethacrylic Acids / Q000737:chemistry*; D013778:Tetrazolium Salts; D013844:Thiazoles; D014162:Transfection / Q000379:methods*; D047392:beta-Cyclodextrins / Q000737:chemistry*
D008689:Methacrylates; D009757:Nylons; D011109:Polymethacrylic Acids; D013778:Tetrazolium Salts; D013844:Thiazoles; D047392:beta-Cyclodextrins; C407037:poly(2-(dimethylamino)ethyl methacrylate); D000073738:2-Hydroxypropyl-beta-cyclodextrin; C042535:polyglycidyl methacrylate; D019856:Ethanolamine; C022616:thiazolyl blue
2-hydroxypropyl-β-cyclodextrin; ATRP; EA; GMA; PDMAEMA; click reaction; gene delivery