Feasibility of marrow harvesting from pediatric sibling donors without hematopoietic growth factors and allotransfusion.
We retrospectively studied 108 marrow harvests from 105 pediatric sibling donors. The median age of donors was 8 years (range: 1-15) and the median body weight was 27 kg (range: 10-100). The volumes of aspirated marrow were 5.0-23.8 mL/kg donor body weight, and harvested bone marrow volume exceeded 15 mL/kg in 42% of the donors. A total of 100 autologous blood donations were performed, and eight donors had red cells salvaged from their harvests reinfused. The median Hb levels before and after harvests were 12.3 g/dL (range: 10.0-14.7) and 11.0 g/dL (range: 8.9-13.8), respectively. None of the donors received allogeneic blood transfusions or hematopoietic growth factors such as EPO and G-CSF before or after collection. Transplanted dose was 1.4-10.8 × 10(8) cells/kg recipient body weight without differences due to donor age. Higher concentrations of nucleated and CD34(+) cells were obtained from younger donors. All donors tolerated the procedures well, with no serious complications. Thus, children may safely donate marrow for allogeneic transplantation, and the yields of nucleated cells for engraftment are substantial.
Department of Cell Transplantation and Regenerative Medicine, Tokai University Hospital, Isehara, Japan.
M
M
Yabe
Department of Pediatrics, Tokai University Hospital, Isehara, Japan.
T
T
Morimoto
Department of Pediatrics, Tokai University Hospital, Isehara, Japan.
T
T
Shimizu
Department of Pediatrics, Tokai University Hospital, Isehara, Japan.
T
T
Koike
Department of Pediatrics, Tokai University Hospital, Isehara, Japan.
H
H
Takakura
Department of Pediatrics, Tokai University Hospital, Isehara, Japan.
K
K
Ohtsubo
Department of Pediatrics, Tokai University Hospital, Isehara, Japan.
A
A
Fukumura
Department of Cell Transplantation and Regenerative Medicine, Tokai University Hospital, Isehara, Japan.
S
S
Kato
Department of Cell Transplantation and Regenerative Medicine, Tokai University Hospital, Isehara, Japan.
H
H
Yabe
D000293:Adolescent; D001804:Blood Transfusion, Autologous / Q000379:methods*; D016026:Bone Marrow Transplantation / Q000379:methods*; D002648:Child; D002675:Child, Preschool; D005240:Feasibility Studies; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D012189:Retrospective Studies; D035781:Siblings; D014019:Tissue Donors