[Liquid-based cervical cytology ThinPrep screening in Hungary].
BACKGROUND
Liquid-based cervical cytology has been developed as an alternative for conventional Papanicolaou cervical cytology.


OBJECTIVE
The aim of this study was to determine the quality assurance of liquid-based cervical cytology.


METHODS
4573 cervical cytology smears were classified according to the Bethesda (2001) system. Human papilloma virus infection was detected and subtyped from reflex test using real-time polymerase chain reaction.


RESULTS
4573 smears were classified according to the Bethesda (2001) system. Negative diagnosis was made in 2323 cases (50.8%), non neoplastic in 2017 cases (44.1%), and positive for intraepithelial lesions or malignancy in 233 cases (5.1%). Unsatisfactory smear for diagnosis was found in 43 cases (0.9%), low-grade squamous intraepithelial lesion in 87 cases (1.9%), high-grade squamous intraepithelial lesion in 24 cases (0.5%), and carcinoma in 23 cases (0.5%). Fifty-nine of the cases were histologically verified and 4 falsely negative cases were detected. The sensitivity, specificity and the positive predictive value were 93.2%, 100% and 100%, respectively. Compared to an identical time periods of the previous three years, the low- and high-grade squamous intraepithelial lesion increased from 0.82% to 2.51%. Eighty one human papilloma virus tests were performed with a positive predictive value of 99%.


CONCLUSIONS
The auditing values of the liquid-bases cervical cytology results meet the proposed threshold values. Liquid-bases cervical cytology is an alternative cervical cytology and it seems to be significantly better than conventional Papanicolaou cervical cytology in all parameters.
Országos Onkológiai Intézet Budapest Ráth György u. 7-9. 1122.
Mihály
M
Bak
Országos Onkológiai Intézet Budapest Ráth György u. 7-9. 1122.
Mária
M
Séberné Éll
Országos Onkológiai Intézet Budapest Ráth György u. 7-9. 1122.
Melinda
M
Bóka
Országos Onkológiai Intézet Budapest Ráth György u. 7-9. 1122.
Zsuzsanna
Z
Veleczki
Szegedi Tudományegyetem, Általános Orvostudományi Kar Orvosi, Fizikai és Orvosi Informatikai Intézet Szeged.
Tibor
T
Nyári
Országos Onkológiai Intézet Budapest Ráth György u. 7-9. 1122.
Imre
I
Pete
Országos Onkológiai Intézet Budapest Ráth György u. 7-9. 1122.
Zoltán
Z
Szentirmay
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002584:Cervix Uteri / Q000473:pathology* / Q000821:virology*; D003588:Cytopathogenic Effect, Viral; D055088:Early Detection of Cancer / Q000379:methods*; D005260:Female; D006801:Humans; D006814:Hungary; D008403:Mass Screening / Q000379:methods*; D008875:Middle Aged; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000175:diagnosis*; D011237:Predictive Value of Tests; D060888:Real-Time Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D002583:Uterine Cervical Neoplasms / Q000517:prevention & control / Q000821:virology; D014626:Vaginal Smears / Q000379:methods*
Bethesda (2001); HPV-DNS-meghatározás (reflexteszt); Human Papilloma Virus DNA test; TBS 2001; ThinPrep; citohisztológiai korreláció; cyto-histological correlation; folyadékalapú (liquid-based) cervixcitológia; liquid-based cervical cytology; minőségbiztosítás; quality assurance