Sample preparation for single cell transcriptomics: essential oil glands in Citrus fruit peel as an example.
Many plant natural products are synthesized in specialized cells and tissues. To learn more about metabolism in these cells, they have to be studied in isolation. Here, we describe a protocol for the isolation of epithelial cells that surround secretory cavities in Citrus fruit peel. Cells isolated using laser microdissection are suitable for RNA isolation and downstream transcriptome analyses.
Institute of Biological Chemistry, M.J. Murdock Metabolomics Laboratory, Washington State University, Clark Hall, Room 341, Pullman, WA, 99164-6340, USA.
Siau Sie
SS
Voo
Bernd Markus
BM
Lange
D053000:Analytic Sample Preparation Methods / Q000379:methods*; D002469:Cell Separation; D002957:Citrus / Q000166:cytology* / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling / Q000379:methods*; D009822:Oils, Volatile / Q000378:metabolism*; D019441:Plant Epidermis / Q000166:cytology*; D018749:RNA, Plant / Q000235:genetics / Q000302:isolation & purification; D059010:Single-Cell Analysis / Q000379:methods*; D016610:Tissue Embedding; D016707:Tissue Fixation
D009822:Oils, Volatile; D018749:RNA, Plant