Exploring the interactome: microfluidic isolation of proteins and interacting partners for quantitative analysis by electron microscopy.
Multimolecular protein complexes are important for many cellular processes. However, the stochastic nature of the cellular interactome makes the experimental detection of complex protein assemblies difficult and quantitative analysis at the single molecule level essential. Here, we present a fast and simple microfluidic method for (i) the quantitative isolation of endogenous levels of untagged protein complexes from minute volumes of cell lysates under close to physiological conditions and (ii) the labeling of specific components constituting these complexes. The method presented uses specific antibodies that are conjugated via a photocleavable linker to magnetic beads that are trapped in microcapillaries to immobilize the target proteins. Proteins are released by photocleavage, eluted, and subsequently analyzed by quantitative transmission electron microscopy at the single molecule level. Additionally, before photocleavage, immunogold can be employed to label proteins that interact with the primary target protein. Thus, the presented method provides a new way to study the interactome and, in combination with single molecule transmission electron microscopy, to structurally characterize the large, dynamic, heterogeneous multimolecular protein complexes formed.
Center for Cellular Imaging and Nano Analytics, Biozentrum, University of Basel , Basel 4056, Switzerland.
Dominic
D
Giss
Simon
S
Kemmerling
Venkata
V
Dandey
Henning
H
Stahlberg
Thomas
T
Braun
D000818:Animals; D019921:Biotinylation; D002460:Cell Line; D006224:Cricetinae; D006801:Humans; D008280:Magnetics; D044085:Microfluidics / Q000379:methods*; D046529:Microscopy, Electron, Transmission; D025941:Protein Interaction Mapping / Q000379:methods*; D011506:Proteins / Q000302:isolation & purification*
D011506:Proteins