Mass spectrometry-based proteomic approach in Oenococcus oeni enological starter.
A simple procedure is proposed for selective protein solubilization and trypsin digestion, followed by off-line liquid chromatography-matrix assisted laser desorption ionization mass spectrometry (LC-MALDI MS) analysis of Oenococcus oeni (O. oeni) bacterium. Peptides were identified from tryptic digests using sequencing by tandem mass spectrometry and database searches. Cytoplasmic and membrane related proteins (MRP) were identified in the O. oeni bacterium. MS/MS data analysis points out 13 peptides having one point mutation from 9 proteins. The major microheterogeneity was found for Zn-dependent alcohol dehydrogenase (Zn-ADH, Q04GE6) and 60 kDa chaperonin (GroEL, Q04E64) that are involved in methionine catabolism and post-translational protein folding, respectively. MS/MS data processing also leads to the identification of 34 unique phosphorylation sites from 19 phosphoproteins.
Department of Chemistry and Chemical Technologies, University of Calabria , Via P. Bucci, Cubo 12/D, 87036 Arcavacata di Rende (CS), Italy.
Anna
A
Napoli
Donatella
D
Aiello
Gilda
G
Aiello
Maria Stella
MS
Cappello
Leonardo
L
Di Donna
Fabio
F
Mazzotti
Stefano
S
Materazzi
Marco
M
Fiorillo
Giovanni
G
Sindona
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D008969:Molecular Sequence Data; D056585:Oenococcus / Q000378:metabolism*; D010750:Phosphoproteins / Q000302:isolation & purification / Q000378:metabolism; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D059748:Proteolysis; D020543:Proteome / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry; D014357:Trypsin / Q000737:chemistry; D014920:Wine / Q000382:microbiology
D001426:Bacterial Proteins; D010750:Phosphoproteins; D020543:Proteome; D014357:Trypsin