Temporal variation in methanogen communities of four different full-scale anaerobic digesters treating food waste-recycling wastewater.
Methanogen communities were investigated using 454 pyrosequencing in four different full-scale anaerobic digesters treating food waste-recycling wastewater. Seasonal samples were collected for 2 years, and 24 samples were available for microbial analysis from a plug flow thermophilic (PT) digester, a continuously-stirred tank thermophilic (CT) digester, an upflow anerobic sludge blanket mesophilic (UM) digester, and a continuously-stirred tank mesophilic (CM) digester. Methanoculleus, Methanobacterium, Methanothermobacter, and Methanosaeta were revealed to be key methanogens in full-scale anaerobic digestion process treating food waste-recycling wastewater. In the PT digester, Methanoculleus was dominant (96.8%). In the CT digester, Methanoculleus was dominant (95.4%) during the first year of operation, but the dominant genus was shifted to Methanothermobacter (98.5%) due to pH increase. In the UM digester, Methanosaeta was dominant (87.2%). In the CM digester, Methanoculleus was constantly dominant (74.8%) except during CM5 when Methanosaeta was dominant (62.6%) due to the low residual acetate concentration (0.1 g/L).
School of Environmental Science and Engineering, Pohang University of Science and Technology, Pohang, Gyeongbuk 790-784, Republic of Korea.
Joonyeob
J
Lee
School of Environmental Science and Engineering, Pohang University of Science and Technology, Pohang, Gyeongbuk 790-784, Republic of Korea.
Byungchul
B
Hwang
School of Environmental Science and Engineering, Pohang University of Science and Technology, Pohang, Gyeongbuk 790-784, Republic of Korea.
Taewoan
T
Koo
Infrastructure and Environment Division, School of Engineering, University of Glasgow, Glasgow G12 8LT, UK.
Seung Gu
SG
Shin
Department of Chemical and Biomolecular Engineering, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea.
Woong
W
Kim
School of Environmental Science and Engineering, Pohang University of Science and Technology, Pohang, Gyeongbuk 790-784, Republic of Korea. Electronic address: shwang@postech.ac.kr.
Seokhwan
S
Hwang
D000693:Anaerobiosis; D001105:Archaea / Q000378:metabolism*; D001419:Bacteria / Q000378:metabolism*; D019149:Bioreactors / Q000382:microbiology*; D005502:Food; D008697:Methane / Q000378:metabolism*; D059027:Recycling; D017422:Sequence Analysis, DNA; D013696:Temperature; D013997:Time Factors; D062065:Wastewater / Q000382:microbiology*; D018508:Water Purification / Q000295:instrumentation*
D008697:Methane; D062065:Wastewater
454 pyrosequencing; Anaerobic digestion; Food waste-recycling wastewater; Full-scale; Methanogen community