Antioxidant activity of gallic acid and methyl gallate in triacylglycerols of Kilka fish oil and its oil-in-water emulsion.
The anti-DPPH radical effect as well as anti-peroxide activity of gallic acid, methyl gallate, and α-tocopherol in a bulk Kilka fish oil and its oil-in-water emulsion stabilized by soy protein isolate at 55°C were investigated. Gallic acid with the lowest hydrophobicity (log P=-0.28) was found to be the most active antiradical agent (IC50=29.5 μM), followed by methyl gallate (IC50=38.0 μM, log P=-0.23) and α-tocopherol (IC50=105.3 μM, log P=0.70). The anti-peroxide activity in the bulk oil system decreased in the order of methyl gallate>gallic acid>α-tocopherol. In the emulsion system, methyl gallate still behaved better than gallic acid, but the highest activity belonged to α-tocopherol. Based on the calculation of a number of kinetic parameters, the antioxidants, in general, showed better performances in the bulk oil system than in the emulsion system.
Ferdowsi University of Mashhad, Faculty of Agriculture, Department of Food Science and Technology, P.O. Box 91775-1163, Mashhad, Iran.
Maryam
M
Asnaashari
Ferdowsi University of Mashhad, Faculty of Agriculture, Department of Food Science and Technology, P.O. Box 91775-1163, Mashhad, Iran. Electronic address: rfarhoosh@um.ac.ir.
Reza
R
Farhoosh
Ferdowsi University of Mashhad, Faculty of Agriculture, Department of Food Science and Technology, P.O. Box 91775-1163, Mashhad, Iran.
Ali
A
Sharif
D000818:Animals; D000975:Antioxidants / Q000737:chemistry*; D001713:Biphenyl Compounds / Q000737:chemistry; D004655:Emulsions; D005395:Fish Oils / Q000032:analysis*; D005399:Fishes; D016166:Free Radical Scavengers / Q000737:chemistry; D005707:Gallic Acid / Q000031:analogs & derivatives* / Q000032:analysis* / Q000737:chemistry; D047348:Hydrolyzable Tannins / Q000737:chemistry; D020128:Inhibitory Concentration 50; D008055:Lipids / Q000737:chemistry; D010545:Peroxides / Q000737:chemistry; D010851:Picrates / Q000737:chemistry; D014280:Triglycerides / Q000737:chemistry*; D024502:alpha-Tocopherol / Q000737:chemistry
D000975:Antioxidants; D001713:Biphenyl Compounds; D004655:Emulsions; D005395:Fish Oils; D016166:Free Radical Scavengers; D047348:Hydrolyzable Tannins; D008055:Lipids; D010545:Peroxides; D010851:Picrates; D014280:Triglycerides; C052082:methyl gallate; D005707:Gallic Acid; C004931:1,1-diphenyl-2-picrylhydrazyl; D024502:alpha-Tocopherol
Antioxidant; Emulsion; Gallic acid; Kilka oil; Methyl gallate; Polar paradox