Naturally derived biofunctional nanofibrous scaffold for skin tissue regeneration.
Significant wound healing activity of Aloe vera (AV) and higher elastic strength of Silk fibroin (SF) along with mammalian cell compatibility makes AV and SF an attractive material for tissue engineering. The purpose of the present work was to combine their unique properties, with the advantage of electrospinning to prepare a hybrid transdermal biomaterial for dermal substitutes. The physico-chemical characterization of the developed scaffold showed finer morphology expressing amino and esteric groups with improved hydrophilic properties and favorable tensile strain of 116% desirable for skin tissue engineering. Their biological response showed favorable fibroblast proliferation compared to control which almost increased linearly by (p<0.01) 34.68% on day 3, (p<0.01) 19.13% on day 6, and (p<0.001) 97.86% on day 9 with higher expression of CMFDA, collagen and F-actin proteins. The obtained results prove that the nanofibrous scaffold with synergistic property of AV and SF would be a potential biomaterial for skin tissue regeneration.
Department of Textile Technology, Anna University, Chennai, India; Centre for Biotechnology, Anna University, Chennai, India.
S
S
Suganya
Nanoscience and Nanotechnology Initiative, Faculty of Engineering, National University of Singapore, Singapore, Singapore.
J
J
Venugopal
Nanoscience and Nanotechnology Initiative, Faculty of Engineering, National University of Singapore, Singapore, Singapore.
S
S
Ramakrishna
Centre for Biotechnology, Anna University, Chennai, India.
B S
BS
Lakshmi
Department of Textile Technology, Anna University, Chennai, India. Electronic address: vrgiridev@yahoo.com.
V R Giri
VR
Dev
D000199:Actins / Q000378:metabolism; D000504:Aloe / Q000737:chemistry; D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D049109:Cell Proliferation; D003094:Collagen / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D005349:Fibroins / Q000737:chemistry; D005452:Fluoresceins / Q000378:metabolism; D006801:Humans; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D012038:Regeneration / Q000187:drug effects*; D012867:Skin / Q000187:drug effects*; D017550:Spectroscopy, Fourier Transform Infrared; D013194:Staining and Labeling; D013718:Tensile Strength / Q000187:drug effects; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D014867:Water / Q000737:chemistry
D000199:Actins; D001672:Biocompatible Materials; D005452:Fluoresceins; D014867:Water; C069306:5-chloromethylfluorescein; D003094:Collagen; D005349:Fibroins
Aloe vera; Silk fibroin; Skin tissue regeneration