[Simultaneous determination of madecassoside, asiaticoside and their aglycones in Centella asiatica (L.) urban extracts by RP-HPLC].
To establish a HPLC method for simultaneous determination of four major constituents (madecassoside, asiaticoside, madecassic acid and asiatic acid) in Centella asiatica (L.) urban extracts.



The analysis was performed on an Agilent 1100 HPLC system with a ZORBAX Eclipse XDB-C8 column (4.6 mm×150 mm, 5μm). The four major constituents were separated with gradient mobile phase that consists of 1mmol/L potassium dihydrogen phosphate and acetonitrile at the detection wavelength of 205 nm.



The four major constituents all had good linear response in the determination ranges (R(2)≥0.9998). The average recoveries (n=9) were 97.4%, 93.7%, 97.5% and 99.8% with RSDs of 3.4%, 1.4%, 4.7% and 4.4%, respectively.



The developed method is sensitive and has good reproducibility, which can be used as a reference for quality control of Centella asiatica (L.) urban extracts.
Laboratory of Pharmaceutical Analysis and Drug Metabolism, College of Pharmaceutical Sciences, Zhejiang University; Zhejiang Provincial Key Laboratory of Anti-Cancer Drug Research, Hangzhou 310058, China.
Qian
Q
Shen
Laboratory of Pharmaceutical Analysis and Drug Metabolism, College of Pharmaceutical Sciences, Zhejiang University; Zhejiang Provincial Key Laboratory of Anti-Cancer Drug Research, Hangzhou 310058, China.
Lu-shan
LS
Yu
Laboratory of Pharmaceutical Analysis and Drug Metabolism, Zhejiang Provincial Key Laboratory of Anti-Cancer Drug Research, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.
Hui
H
Zhou
Laboratory of Pharmaceutical Analysis and Drug Metabolism, College of Pharmaceutical Sciences, Zhejiang University; Zhejiang Provincial Key Laboratory of Anti-Cancer Drug Research, Hangzhou 310058, China.
Su
S
Zeng
D028041:Centella / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D053978:Pentacyclic Triterpenes / Q000032:analysis; D010936:Plant Extracts / Q000032:analysis; D015203:Reproducibility of Results; D014315:Triterpenes / Q000032:analysis*
D053978:Pentacyclic Triterpenes; D010936:Plant Extracts; D014315:Triterpenes; C017032:asiatic acid; C093443:madecassoside; C001669:madecassic acid; C004446:asiaticoside