Carcino-embryonic antigen detection based on fluorescence resonance energy transfer between quantum dots and graphene oxide.
The mixture of graphene oxide (GO) and aptamer labeled fluorophore is widely used in developing fluorescent sensors for the analysis of biomolecules, according to the light signal 'off-on' procedure. Moreover, the laser-induced fluorescence-coupled affinity probe capillary electrophoresis (APCE) technique has been broadly applied for the separation of micromolecules. Here, a strategy is proposed for analysis of content of carcino-embryonic antigen (CEA) based on the combination of GO and quantum dots labeling aptamer (QD-aptamer) by capillary electrophoresis (CE). The method has three advantages: (i) combined with CE, only few samples are required and efficiency of separation is high, (ii) fluorescent detection can be carried out after separation of GO and fluorescence probe combined with targets by CE, while fluorescence detection sensitivity had been greatly improved, and (iii) the issues of APCE, including the effect of excess fluorescence probe and maximizing separation between analytes, could be solved by introducing GO. It has been proved that QD-aptamer-CEA complex can completely dissociate from GO. Results show that the fluorescence intensity has a linear relationship with the concentration of CEA in the range from 0.257 to 12.9 ng/mL, and the limit of detection is approximately 5 pg/mL (S/N=3). The proposed method with high specificity has been applied for the accurate analysis of content of CEA in patient׳s serum.
Britton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics-Hubei Bioinformatics & Molecular Imaging Key Laboratory, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, PR China.
Zi-Ming
ZM
Zhou
Department of Clinical Laboratory, Wuhan Commercial Staff Hospital, Wuhan 430021, PR China.
Jun
J
Zhou
Britton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics-Hubei Bioinformatics & Molecular Imaging Key Laboratory, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, PR China.
Jun
J
Chen
Britton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics-Hubei Bioinformatics & Molecular Imaging Key Laboratory, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, PR China.
Rong-Na
RN
Yu
Britton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics-Hubei Bioinformatics & Molecular Imaging Key Laboratory, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, PR China.
Ming-Zhen
MZ
Zhang
Britton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics-Hubei Bioinformatics & Molecular Imaging Key Laboratory, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, PR China.
Ji-Tao
JT
Song
Britton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics-Hubei Bioinformatics & Molecular Imaging Key Laboratory, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, PR China. Electronic address: zydi@mail.hust.edu.cn.
Yuan-Di
YD
Zhao
D052157:Aptamers, Nucleotide / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods; D002272:Carcinoembryonic Antigen / Q000097:blood*; D019075:Electrophoresis, Capillary / Q000379:methods; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D010087:Oxides / Q000737:chemistry*; D045663:Quantum Dots / Q000737:chemistry*
D052157:Aptamers, Nucleotide; D002272:Carcinoembryonic Antigen; D010087:Oxides; D006108:Graphite
Aptamer; Capillary electrophoresis; Carcino-embryonic antigen; Graphene oxide; Quantum dots