Determination of aflatoxins in rice samples by ultrasound-assisted matrix solid-phase dispersion.
This work describes the application of ultrasound-assisted matrix solid-phase dispersion as an extraction and sample preparation approach for aflatoxins (B1, B2, G1 and G2) and subsequent determination of them by high-performance liquid chromatography-fluorescence detection. A Box-Behnken design in combination with response surface methodology was used to determine the affecting parameters on the extraction procedure. The influence of different variables including type of dispersing phase, sample-to-dispersing phase ratio, type and quantity of clean-up phase, ultrasonication time, ultrasonication temperature, nature and volume of the elution solvent was investigated in the optimization study. C18, primary-secondary amine (PSA) and acetonitrile were selected as dispersing phase, clean-up phase and elution solvent, respectively. The obtained optimized values were sample-to-dispersing phase ratio of 1 : 1, 60 mg of PSA, 11 min ultrasonication time, 30°C ultrasonication temperature and 4 mL acetonitrile. Under the optimal conditions, the limits of detection were ranged from 0.09 to 0.14 ng g(-1) and the precisions [relative standard deviation (RSD%)] were <8.6%. The recoveries of the matrix solid-phase dispersion process ranged from 78 to 83% with RSD <10% in all cases. Finally, this method was successfully applied to the extraction of trace amounts of aflatoxins in rice samples.
Department of Chemistry, Central Tehran Branch, Islamic Azad University, PO Box 14676-86831, Tehran, Iran dr.manoochehri@yahoo.com.
Mahboobeh
M
Manoochehri
Young Researchers and Elite Club, Karaj Branch, Islamic Azad University, PO Box 31485-313, Karaj, Iran.
Ali Akbar
AA
Asgharinezhad
Pirayeh Zist Laboratory, Qazvin, Iran.
Mahdi
M
Safaei
D000348:Aflatoxins / Q000032:analysis* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D005506:Food Contamination / Q000032:analysis; D012275:Oryza / Q000737:chemistry*; D014465:Ultrasonics / Q000379:methods*
D000348:Aflatoxins