Synthesis and evaluation of turn-on fluorescent probes for imaging steroid sulfatase activities in cells.
We have synthesized and evaluated two self-immobilizing, turn-on fluorescent probes carrying a coumarin molecular framework for imaging intracellular human steroid sulfatase (STS) activity. The 8-fluoromethyl coumarin derivative, which gives stronger fluorescence response in the incubation study with STS preparations, was successfully applied to visualize STS activity in cells.
Department of Chemistry, National Taiwan University, Taipei 106, Taiwan. lclo@ntu.edu.tw.
Chih-Hsuan
CH
Tai
Chun-Ping
CP
Lu
Shih-Hsiung
SH
Wu
Lee-Chiang
LC
Lo
D002460:Cell Line; D004789:Enzyme Activation; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry*; D006801:Humans; D015394:Molecular Structure; D043266:Steryl-Sulfatase / Q000032:analysis / Q000378:metabolism*
D005456:Fluorescent Dyes; D043266:Steryl-Sulfatase