Production of fumaric acid from biodiesel-derived crude glycerol by Rhizopus arrhizus.
This work investigated the capability of Rhizopus arrhizus to assimilate biodiesel-derived crude glycerol and convert it into fumaric acid. After optimizing the initial glycerol concentration, spore inoculum and yeast extract concentration, smaller pellets (0.7 mm) and higher biomass (3.11 g/L) were obtained when R. arrhizus grew on crude glycerol. It was found that crude glycerol was more suitable than glucose for smaller R. arrhizus pellet forming. When 80 g/L crude glycerol was used as carbon source, the fumaric acid production of 4.37 g/L was obtained at 192 h. With a highest concentration of 22.81 g/L achieved in the co-fermentation of crude glycerol (40 g/L) and glucose (40 g/L) at 144 h, the fumaric acid production was enhanced by 553.6%, compared to the fermentation using glycerol (80 g/L) as sole carbon source. Moreover, the production cost of fumaric acid in co-fermentation was reduced by approximately 14% compared to glucose fermentation.
Beijing Bioprocess Key Laboratory, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.
Yuqing
Y
Zhou
Beijing Bioprocess Key Laboratory, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China; Amoy - BUCT Industrial Bio-technovation Institute, Amoy 361022, PR China.
Kaili
K
Nie
Beijing Bioprocess Key Laboratory, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.
Xin
X
Zhang
Beijing Bioprocess Key Laboratory, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.
Shihong
S
Liu
Beijing Bioprocess Key Laboratory, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.
Meng
M
Wang
Beijing Bioprocess Key Laboratory, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China; Amoy - BUCT Industrial Bio-technovation Institute, Amoy 361022, PR China. Electronic address: dengli@mail.buct.edu.cn.
Li
L
Deng
Beijing Bioprocess Key Laboratory, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China; State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, PR China.
Fang
F
Wang
Beijing Bioprocess Key Laboratory, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.
Tianwei
T
Tan
D056804:Biofuels; D018533:Biomass; D003470:Culture Media; D005285:Fermentation; D005650:Fumarates / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism*; D012233:Rhizopus / Q000378:metabolism*
D056804:Biofuels; D003470:Culture Media; D005650:Fumarates; C032005:fumaric acid; D005947:Glucose; D005990:Glycerol
Co-fermentation; Crude glycerol; Fumaric acid; Pellet; Rhizopus arrhizus