Correlation between pesticide resistance and enzyme activity in the diamondback moth, Plutella xylostella.
The diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae), is one of the most important pests that has developed high pesticide resistance. The resistances of five Chinese populations of this moth, four resistant strains (from Beijing, Henan, Fujian, and Guangdong) and one susceptible strain, to five pesticides were determined, and the activities of carboxylesterase, glutathione S-transferase, and acetylcholine esterase were tested in all five populations. The correlations between pesticide resistance and enzyme activity were analyzed. The results showed that the resistance status to the five pesticides was different among the five populations. The resistance ratios of the Beijing and Henan populations to spinosad were 5.84 and 8.22, respectively, and those to beta-cypermethrin were 4.91 and 4.98, respectively. These ratios were higher than those for the Fujian and Guangdong populations. The Fujian population was more sensitive to abamectin and chlorpyrifos than the susceptible population (the resistance ratios were 0.14 and 0.91, respectively); in fact, the median lethal concentration for P. xylostella was significantly higher for chlorpyrifos than that for any of the other four pesticides. The carboxylesterase activity in P. xylostella showed positive correlations with the resistance to spinosad, beta-cypermethrin, chlorpyrifos, and abamectin, but no correlation was observed between the carboxylesterase activity and resistance to emamectin benzoate, between glutathione S-transferase activity and resistance to any of the five pesticides tested, or between acetylcholine esterase activity and any of the pesticides except for emamectin benzoate.
Institute of Plant and Environmental Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China.
Ya-Jun
YJ
Gong
Ze-Hua
ZH
Wang
Bao-Cai
BC
Shi
Zong-Jiang
ZJ
Kang
Liang
L
Zhu
Gui-Hua
GH
Jin
Shu-Jun
SJ
Weig
D000110:Acetylcholinesterase / Q000235:genetics / Q000378:metabolism; D000818:Animals; D043182:Carboxylesterase / Q000235:genetics / Q000378:metabolism; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism; D007305:Insecticide Resistance; D007814:Larva / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D009036:Moths / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development
D019476:Insect Proteins; D005982:Glutathione Transferase; D043182:Carboxylesterase; D000110:Acetylcholinesterase