Simultaneous saccharification and fermentation of cassava to succinic acid by Escherichia coli NZN111.
In this study, the production of succinic acid from cassava starch and raw cassava instead of glucose by Escherichia coli NZN111 was investigated. During the two-stage fermentation, simultaneous saccharification and fermentation (SSF) was applied in the anaerobic stage. The results showed that both the productivity and specific productivity in the process conducted at 40°C were higher than those in the cultivation conducted at 37°C. The yield of succinic acid based on the amount of added starch reached the highest level 0.86 g/g and cassava starch was almost totally hydrolyzed in the SSF process. With the improved cell density, 127.13 g/L of succinic acid was obtained. When the liquefied crude cassava powder was used directly in SSF, 106.17 g/L of succinic acid was formed. The result showed that crude cassava powder could be another cheap raw material for succinic acid formation.
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China.
Cuixia
C
Chen
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China.
Shaopeng
S
Ding
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China.
Dezheng
D
Wang
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China. Electronic address: lizm@ecust.edu.cn.
Zhimin
Z
Li
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China.
Qin
Q
Ye
D002241:Carbohydrates / Q000737:chemistry*; D004926:Escherichia coli / Q000378:metabolism*; D005285:Fermentation; D002365:Manihot / Q000378:metabolism*; D019802:Succinic Acid / Q000378:metabolism*
D002241:Carbohydrates; D019802:Succinic Acid
Cassava; Escherichia coli NZN111; Simultaneous saccharification and fermentation; Succinic acid