Survey of aflatoxins in rice from Iran using immunoaffinity column clean-up and HPLC with fluorescence detection.
A study was undertaken to determine levels of aflatoxins in rice. A total of 261 rice samples were analyzed by HPLC using a method was based on the extraction of 50 g of finely ground rice plus 5 g NaCl with 200 ml of 80% methanol. After filtration and immunoaffinity clean-up, 20 µl was injected onto the HPLC. HPLC analysis was carried out using a Genesis RP C18 column (250 × 4.6, 4 µm I.D.) and a mobile phase with a linear gradient of water/methanol/acetonitrile (6 : 2 : 2 v/v) over 16 min. Aflatoxins were determined after post-column derivatisation with iodine by fluorescence detection at excitation and emission wavelengths of 365 and 445 nm, respectively. It was found that 68.9% of the rice samples contained aflatoxin B1 at levels greater than 0.2 ng g(-1).
a Testa Quality Control Laboratory, North-East Food, Industrial Technology and Biotechnology Park , Mashhad , Iran.
J
J
Feizy
H R
HR
Beheshti
N Khoshbakht
NK
Fahim
S S Fakoor
SS
Janati
G
G
Davari
D016604:Aflatoxin B1 / Q000032:analysis; D000348:Aflatoxins / Q000032:analysis*; D000622:Aminolevulinic Acid / Q000031:analogs & derivatives* / Q000737:chemistry; D002846:Chromatography, Affinity / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D007492:Iran; D008452:Maximum Allowable Concentration; D012639:Seeds / Q000737:chemistry; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence
D000348:Aflatoxins; C058222:2-amino-5-hydroxy-4-oxopentanoic acid; D000622:Aminolevulinic Acid; D016604:Aflatoxin B1
aflatoxins; chromatography, HPLC; quality assurance; rice; risk assessment; toxicology