Targeted genomic rearrangements using CRISPR/Cas technology.
Genomic rearrangements are frequently observed in cancer cells but have been difficult to generate in a highly specific manner for functional analysis. Here we report the application of CRISPR/Cas technology to successfully generate several types of chromosomal rearrangements implicated as driver events in lung cancer, including the CD74-ROS1 translocation event and the EML4-ALK and KIF5B-RET inversion events. Our results demonstrate that Cas9-induced DNA breaks promote efficient rearrangement between pairs of targeted loci, providing a highly tractable approach for the study of genomic rearrangements.
1] Dana-Farber Cancer Institute, Department of Medical Oncology, Boston, Massachusetts 02215, USA [2] Broad Institute of MIT and Harvard, Cancer Program, Cambridge, Massachusetts 02142, USA.
Peter S
PS
Choi
1] Dana-Farber Cancer Institute, Department of Medical Oncology, Boston, Massachusetts 02215, USA [2] Broad Institute of MIT and Harvard, Cancer Program, Cambridge, Massachusetts 02142, USA [3] Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA.
Matthew
M
Meyerson
D064112:Clustered Regularly Interspaced Short Palindromic Repeats / Q000235:genetics*; D015321:Gene Rearrangement; D006801:Humans