Antibody against N-terminal domain of phospholipid scramblase 1 induces apoptosis in colorectal cancer cells through the intrinsic apoptotic pathway.
Phospholipid scramblase 1 involve in biological processes including phospholipid movement, proliferation, and apoptosis. Treatment with an antiphospholipid scramblase 1 antibody (NP1) has been demonstrated to inhibit cell proliferation in colorectal cancer. This study aimed to explore the role of NP1 treatment in the apoptosis of colorectal cancer cells. Results showed that NP1 treatment significantly increases the apoptosis of colorectal cancer cells via the activation of caspase 8, caspase 9, and caspase 3. Moreover, pretreatment with a caspase 8 inhibitor did not fully prevent the apoptotic effects of NP1. Taken together, these data indicate NP1 induces cell apoptosis primary through the intrinsic apoptotic pathway. NP1 may serve as a potential therapeutic agent.
Graduate Institute of Biomedical Sciences, Chang Gung University, 259 Wen-Hua 1st Road, Kwei-shan, Taoyuan, Taiwan.
Chun-Yu
CY
Chen
Jinn-Shiun
JS
Chen
Yeh-Pin
YP
Chou
Yung-Bin
YB
Kuo
Chung-Wei
CW
Fan
Err-Cheng
EC
Chan
D000906:Antibodies / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D053181:Caspase 8 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D045744:Cell Line, Tumor; D003106:Colon / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D015179:Colorectal Neoplasms / Q000188:drug therapy* / Q000201:enzymology* / Q000473:pathology; D006801:Humans; D058990:Molecular Targeted Therapy; D049294:Phospholipid Transfer Proteins / Q000737:chemistry / Q000378:metabolism*; D017434:Protein Structure, Tertiary / Q000187:drug effects; D012007:Rectum / Q000187:drug effects / Q000201:enzymology / Q000473:pathology
D000906:Antibodies; C493422:PLSCR1 protein, human; D049294:Phospholipid Transfer Proteins; D053148:Caspase 3; D053181:Caspase 8; D053453:Caspase 9
apoptosis; caspase; colorectal cancer; phospholipid scramblase 1