Cloning and expression analysis of litchi (Litchi Chinensis Sonn.) polyphenol oxidase gene and relationship with postharvest pericarp browning.
Polyphenol oxidase (PPO) plays a key role in the postharvest pericarp browning of litchi fruit, but its underlying mechanism remains unclear. In this study, we cloned the litchi PPO gene (LcPPO, JF926153), and described its expression patterns. The LcPPO cDNA sequence was 2120 bps in length with an open reading frame (ORF) of 1800 bps. The ORF encoded a polypeptide with 599 amino acid residues, sharing high similarities with other plant PPO. The DNA sequence of the ORF contained a 215-bp intron. After carrying out quantitative RT-PCR, we proved that the LcPPO expression was tissue-specific, exhibiting the highest level in the flower and leaf. In the pericarp of newly-harvested litchi fruits, the LcPPO expression level was relatively high compared with developing fruits. Regardless of the litchi cultivar and treatment conditions, the LcPPO expression level and the PPO activity in pericarp of postharvest fruits exhibited the similar variations. When the fruits were stored at room temperature without packaging, all the pericarp browning index, PPO activity and the LcPPO expression level of litchi pericarps were reaching the highest in Nandaowuhe (the most rapid browning cultivar), but the lowest in Ziniangxi (the slowest browning cultivar) within 2 d postharvest. Preserving the fruits of Feizixiao in 0.2-μm plastic bag at room temperature would decrease the rate of pericarp water loss, delay the pericarp browning, and also cause the reduction of the pericarp PPO activity and LcPPO expression level within 3 d postharvest. In addition, postharvest storage of Feizixiao fruit stored at 4°C delayed the pericarp browning while decreasing the pericarp PPO activity and LcPPO expression level within 2 d after harvest. Thus, we concluded that the up-regulation of LcPPO expression in pericarp at early stage of postharvest storage likely enhanced the PPO activity and further accelerated the postharvest pericarp browning of litchi fruit.
Environment and Plant Protection Research Institute, Chinese Academy of Tropical Agriculture Sciences, Haikou, Hainan, China.
Jiabao
J
Wang
Environment and Plant Protection Research Institute, Chinese Academy of Tropical Agriculture Sciences, Haikou, Hainan, China.
Baohua
B
Liu
Environment and Plant Protection Research Institute, Chinese Academy of Tropical Agriculture Sciences, Haikou, Hainan, China.
Qian
Q
Xiao
Environment and Plant Protection Research Institute, Chinese Academy of Tropical Agriculture Sciences, Haikou, Hainan, China.
Huanling
H
Li
Environment and Plant Protection Research Institute, Chinese Academy of Tropical Agriculture Sciences, Haikou, Hainan, China.
Jinhua
J
Sun
D004156:Catechol Oxidase / Q000096:biosynthesis / Q000235:genetics*; D003001:Cloning, Molecular; D005638:Fruit / Q000201:enzymology / Q000235:genetics*; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D032125:Litchi / Q000201:enzymology / Q000235:genetics*; D016366:Open Reading Frames; D009928:Organ Specificity; D010802:Phylogeny; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D010940:Plant Proteins; D004156:Catechol Oxidase