Reaction mechanisms and rate constants of waste degradation in landfill bioreactor systems with enzymatic-enhancement.
Augmenting leachate before recirculation with peroxidase enzymes is a novel method to increase the available carbon, and therefore the food supply to microorganisms at the declining phase of the anaerobic landfill bioreactor operation. In order to optimize the enzyme-catalyzed leachate recirculation process, it is necessary to identify the reaction mechanisms and determine rate constants. This paper presents a kinetic model developed to ascertain the reaction mechanisms and determine the rate constants for enzyme catalyzed anaerobic waste degradation. The maximum rate of reaction (Vmax) for MnP enzyme-catalyzed reactors was 0.076 g(TOC)/g(DS).day. The catalytic turnover number (k(cat)) of the MnP enzyme-catalyzed was 506.7 per day while the rate constant (k) of the un-catalyzed reaction was 0.012 per day.
Centre for Environmental Engineering Research and Education (CEERE), Schulich School of Engineering, University of Calgary, Calgary, Alberta T2N 1N4, Canada.
P A
PA
Jayasinghe
Centre for Environmental Engineering Research and Education (CEERE), Schulich School of Engineering, University of Calgary, Calgary, Alberta T2N 1N4, Canada. Electronic address: jhettiar@ucalgary.ca.
J P A
JP
Hettiaratchi
Centre for Environmental Engineering Research and Education (CEERE), Schulich School of Engineering, University of Calgary, Calgary, Alberta T2N 1N4, Canada.
A K
AK
Mehrotra
CSIR-National Environmental Engineering Research Institute, Solid and Hazardous Waste Management Division, Nehru Marg, Nagpur 440 020, India.
S
S
Kumar
D001673:Biodegradation, Environmental; D019149:Bioreactors; D002244:Carbon / Q000032:analysis; D007700:Kinetics; D009930:Organic Chemicals / Q000032:analysis; D010544:Peroxidases / Q000378:metabolism*; D013997:Time Factors; D064091:Waste Disposal Facilities
D009930:Organic Chemicals; D002244:Carbon; D010544:Peroxidases; C051129:manganese peroxidase
Enzymatic enhancement; Landfill bioreactors; Lignin degradation; Rate constants; Reaction mechanisms