Determination of 10-chloromethyl-11-demethyl-12-oxo-calanolide A in rat plasma using liquid chromatography-tandem mass spectrometry and its application to pharmacokinetics.
A rapid and specific liquid chromatography-tandem mass spectrometry for the quantitation of 10-chloromethyl-11-demethyl-12-oxo-calanolide A (F18), a small-molecule nonnucleoside reverse transcriptase inhibitor, was developed and validated in rat plasma. F18 was monitored by positive electrospray ionization in the selected reaction monitoring mode. The standard curve was linear over the range of 2-1000 ng/mL. The method was used to determine the plasma concentration of F18 after a single oral dose of 50 mg/kg in rats.
Department of Drug Metabolism, Key Laboratory of Active Substances Discovery and Drug Ability Evaluation, State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100050, People's Republic of China.
Li
L
Sheng
Qinghao
Q
Zhang
Zhihao
Z
Liu
Xin
X
Liu
Baolian
B
Wang
Hui
H
Chen
Yan
Y
Li
D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D005260:Female; D008297:Male; D039681:Pyranocoumarins / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics*; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry / Q000379:methods*
C547679:10-chloromethyl-11-demethyl-12-oxocalanolide A; D039681:Pyranocoumarins
10-chloromethyl-11-demethyl-12-oxo-calanolide A; LC-MS/MS; pharmacokinetics; quantitative determination; rat plasma