A lipid-based method for the preparation of a piezoelectric DNA biosensor.
A piezoelectric DNA biosensor was prepared by immobilizing DNA probes on a quartz crystal microbalance (QCM) using a lipid-based method. A QCM electrode was coated with a hybrid bilayer membrane composed of an octadecanethiol monolayer and a lipid monolayer containing biotinylated lipids to establish biotin groups on the electrode surface. A DNA biosensor was prepared by sequentially immobilizing avidin and the biotinylated probe. The DNA biosensor was stable throughout repeated surface regeneration and showed higher sensitivity than that prepared by the conventional chemical method using diimide. We also optimized the surface regeneration conditions and flow rate for flow injection analysis.
Amogreentech Co. Ltd., Gangneung Science Industry Park, Gangneung 210-340, Republic of Korea.
Sungil
S
Kim
Department of Chemistry, Gangneung-Wonju National University, Gangneung 210-702, Republic of Korea. Electronic address: sjchoi@gwnu.ac.kr.
Suk-Jung
SJ
Choi
D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D004247:DNA / Q000032:analysis*; D015342:DNA Probes / Q000737:chemistry; D004566:Electrodes; D017022:Flow Injection Analysis; D061925:Immobilized Nucleic Acids / Q000737:chemistry; D008055:Lipids / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D058525:Quartz Crystal Microbalance Techniques
D015342:DNA Probes; D061925:Immobilized Nucleic Acids; D008055:Lipids; D004247:DNA
Avidin; Biotin; DNA biosensor; Lipid; Quartz crystal microbalance