PIPKIIα is widely expressed in hematopoietic-derived cells and may play a role in the expression of alpha- and gamma-globins in K562 cells.
Characterized for the first time in erythrocytes, phosphatidylinositol phosphate kinases (PIP kinases) belong to a family of enzymes that generate various lipid messengers and participate in several cellular processes, including gene expression regulation. Recently, the PIPKIIα gene was found to be differentially expressed in reticulocytes from two siblings with hemoglobin H disease, suggesting a possible relationship between PIPKIIα and the production of globins. Here, we investigated PIPKIIα gene and protein expression and protein localization in hematopoietic-derived cells during their differentiation, and the effects of PIPKIIα silencing on K562 cells. PIPKIIα silencing resulted in an increase in α and γ globins and a decrease in the proliferation of K562 cells without affecting cell cycle progression and apoptosis. In conclusion, using a cell line model, we showed that PIPKIIα is widely expressed in hematopoietic-derived cells, is localized in their cytoplasm and nucleus, and is upregulated during erythroid differentiation. We also showed that PIPKIIα silencing can induce α and γ globin expression and decrease cell proliferation in K562 cells.
Department of Clinical Pathology, School of Medical Sciences, University of Campinas - UNICAMP, P.O. Box 6111, Campinas, São Paulo, 13083-970, Brazil.
Vânia
V
Peretti de Albuquerque Wobeto
João Agostinho
JA
Machado-Neto
Tânia Regina
TR
Zaccariotto
Daniela Maria
DM
Ribeiro
Adriana
A
da Silva Santos Duarte
Sara Teresinha Olalla
ST
Saad
Fernando Ferreira
FF
Costa
Maria
M
de Fatima Sonati
D017209:Apoptosis / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics*; D005786:Gene Expression Regulation; D020868:Gene Silencing; D006412:Hematopoietic Stem Cells / Q000378:metabolism; D006801:Humans; D020014:K562 Cells; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000096:biosynthesis* / Q000235:genetics; D055542:alpha-Globins / Q000096:biosynthesis*; D005719:gamma-Globulins / Q000096:biosynthesis*
D055542:alpha-Globins; D005719:gamma-Globulins; C514871:PIP4K2A protein, human; D017853:Phosphotransferases (Alcohol Group Acceptor)