Proteomics and metabolomics for mechanistic insights and biomarker discovery in cardiovascular disease.
In the last decade, proteomics and metabolomics have contributed substantially to our understanding of cardiovascular diseases. The unbiased assessment of pathophysiological processes without a priori assumptions complements other molecular biology techniques that are currently used in a reductionist approach. In this review, we highlight some of the "omics" methods used to assess protein and metabolite changes in cardiovascular disease. A discrete biological function is very rarely attributed to a single molecule; more often it is the combined input of many proteins. In contrast to the reductionist approach, in which molecules are studied individually, "omics" platforms allow the study of more complex interactions in biological systems. Combining proteomics and metabolomics to quantify changes in metabolites and their corresponding enzymes will advance our understanding of pathophysiological mechanisms and aid the identification of novel biomarkers for cardiovascular disease.
King's British Heart Foundation Centre, King's College of London, London, United Kingdom.
Javier
J
Barallobre-Barreiro
Cancer Research UK and EPSRC Cancer Imaging Centre, The Institute of Cancer Research and The Royal Marsden NHS Foundation Trust, Sutton, Surrey, United Kingdom.
Yuen-Li
YL
Chung
King's British Heart Foundation Centre, King's College of London, London, United Kingdom. Electronic address: manuel.mayr@kcl.ac.uk.
Manuel
M
Mayr
D015415:Biomarkers / Q000032:analysis*; D002318:Cardiovascular Diseases / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism; D006801:Humans; D055432:Metabolomics / Q000639:trends*; D040901:Proteomics / Q000639:trends*
D015415:Biomarkers
CVD; Espectrometría de masas; Espectroscopia de resonancia magnética; LC; MRS; MS; Magnetic resonance spectroscopy; Mass spectrometry; Metabolomics; Metabolómica; Proteomics; Proteómica; cardiovascular disease; liquid chromatography; magnetic resonance spectroscopy; mass spectrometry