Effect of 3D chondrocyte culturing conditions on the formation of extracellular matrix in cartilage tissue-engineering constructs.
We studied biochemical, morphological, and histological parameters of the extracellular matrix in scaffold-free tissue engineering chondrotransplants prepared from chondrocytes isolated from knee joint cartilage biopsy specimens of Haflinger horses (age 3.5-14 years) and in transplants prepared on the basis of commercial matrixes of Ethisorb® and Chondro-Gide(®). A total of 50 tissue-engineering constructs were designed and analyzed. Passage 2 cells populations were used. Mechanical stimulation during culturing of scaffold-free constructs considerably activated synthesis of the basic components of the cartilage matrix (proteoglycan concentration was 35% of the content in the native tissue, and the content of collagen-specific amino acids hydroxyproline and hydroxylysine attained 29.3 and 12.7%, respectively). The architecture of these cartilage constructs was morphologically and histologically similar to the native cartilage tissue. Insufficient support of the chondrogenesis by scaffold-based chondrotransplants and no differences between these constructs by the studied parameters were noted despite different chemical nature and structure of these scaffolds.
Research Center for Medical Technology and Biotechnology, Bad Langensalza, Germany, iponomarev@fzmb.de.
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Ponomarev
L M
LM
Kochneva
D
D
Barnewitz
D000818:Animals; D001672:Biocompatible Materials; D002356:Cartilage / Q000166:cytology / Q000502:physiology; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D019902:Chondrocytes / Q000378:metabolism*; D005109:Extracellular Matrix / Q000378:metabolism*; D006025:Glycosaminoglycans / Q000096:biosynthesis; D006736:Horses; D006901:Hydroxylysine / Q000378:metabolism; D006909:Hydroxyproline / Q000378:metabolism; D011100:Polyglycolic Acid; D012038:Regeneration; D023822:Tissue Engineering; D054457:Tissue Scaffolds
D001672:Biocompatible Materials; C460779:Ethisorb; D006025:Glycosaminoglycans; D011100:Polyglycolic Acid; D006901:Hydroxylysine; D006909:Hydroxyproline