Ovarian blood vessel occlusion as a surgical sterilization method in rats.
OBJECTIVE
To evaluate the female sterilization by occlusion of the ovarian blood flow, using the rat as experimental model.


METHODS
Fifty-five females rats were divided into four groups: I (n=10), bilateral ovariectomy, euthanized at 60 or 90 days; II (n=5), opening the abdominal cavity, euthanized at 90 days; III (n=20), bilateral occlusion of the ovarian blood supply using titanium clips, euthanized at 60 or 90 days; and IV (n=20), bilateral occlusion of the ovarian blood supply using nylon thread, euthanized at 60 or 90 days. The estrous cycle was monitored by vaginal cytology. After euthanasia, the reproductive tissues were evaluated histologically.


RESULTS
Ovarian atresia was identified macroscopically at 60 days after surgery in the rats in groups III and IV; however, most of the rats in group III maintained cyclicity. Histology of the tissues from group IV revealed that the ovarian tissue was replaced by dense fibrous connective tissue that was slightly vascularized and that intact follicles were absent by 90 days.


CONCLUSIONS
Ovarian blood vessels occluded caused ischemia, leading to progressive tissue necrosis, and bilateral occlusion using a nylon ligature is a viable method for surgical sterilization.
University of Franca, Franca, SP, Brazil.
Eduardo
E
Murakami
UNIFRAN, Franca, SP, Brazil.
Laíza
L
Sartori de Camargo
University of Franca, Franca, SP, Brazil.
Karym Christine de
KC
Freitas Cardoso
Federal University of Goias, Jatai, GO, Brazil.
Marina Pacheco
MP
Miguel
Department of Preventive Veterinary Medicine and Animal, Reproduction, School of Agrarian Sciences and Veterinary Medicine, Sao Paulo State University, Jaboticabal, SP, Brazil.
Denise Cláudia
DC
Tavares
Veterinary Surgery Division, UNIFRAN, Franca, SP, Brazil.
Cristiane dos
Cd
Santos Honsho
Animal Reproduction Division, UNIFRAN, Franca, SP, Brazil.
Fabiana
F
Ferreira de Souza
D000818:Animals; D005260:Female; D008026:Ligation; D023421:Models, Animal; D009757:Nylons; D010052:Ovariectomy / Q000379:methods; D010053:Ovary / Q000098:blood supply* / Q000473:pathology / Q000601:surgery; D017208:Rats, Wistar; D015203:Reproducibility of Results; D013245:Sterilization, Reproductive / Q000295:instrumentation / Q000379:methods*; D013525:Surgical Instruments; D060205:Therapeutic Occlusion / Q000295:instrumentation / Q000379:methods*; D013997:Time Factors; D014025:Titanium
D009757:Nylons; D014025:Titanium