Use of a semi-field system to evaluate the efficacy of topical repellents under user conditions provides a disease exposure free technique comparable with field data.
BACKGROUND
Before topical repellents can be employed as interventions against arthropod bites, their efficacy must be established. Currently, laboratory or field tests, using human volunteers, are the main methods used for assessing the efficacy of topical repellents. However, laboratory tests are not representative of real life conditions under which repellents are used and field-testing potentially exposes human volunteers to disease. There is, therefore, a need to develop methods to test efficacy of repellents under real life conditions while minimizing volunteer exposure to disease.


METHODS
A lotion-based, 15% N, N-Diethyl-3-methylbenzamide (DEET) repellent and 15% DEET in ethanol were compared to a placebo lotion in a 200 sq m (10 m × 20 m) semi-field system (SFS) against laboratory-reared Anopheles arabiensis mosquitoes and in full field settings against wild malaria vectors and nuisance-biting mosquitoes. The average percentage protection against biting mosquitoes over four hours in the SFS and field setting was determined. A Poisson regression model was then used to determine relative risk of being bitten when wearing either of these repellents compared to the placebo.


RESULTS
Average percentage protection of the lotion-based 15% DEET repellent after four hours of mosquito collection was 82.13% (95% CI 75.94-88.82) in the semi-field experiments and 85.10% (95% CI 78.97-91.70) in the field experiments. Average percentage protection of 15% DEET in ethanol after four hours was 71.29% (CI 61.77-82.28) in the semi-field system and 88.24% (84.45-92.20) in the field.


CONCLUSIONS
Semi-field evaluation results were comparable to full-field evaluations, indicating that such systems could be satisfactorily used in measuring efficacy of topically applied mosquito repellents, thereby avoiding risks of exposure to mosquito-borne pathogens, associated with field testing.
Ifakara Health Institute, Box 74, Bagamoyo, Tanzania. psangoro@ihi.or.tz.
Onyango
O
Sangoro
Dickson
D
Lweitojera
Emmanuel
E
Simfukwe
Hassan
H
Ngonyani
Edgar
E
Mbeyela
Daniel
D
Lugiko
Japhet
J
Kihonda
Marta
M
Maia
Sarah
S
Moore
D000287:Administration, Topical; D000293:Adolescent; D000818:Animals; D000852:Anopheles / Q000187:drug effects* / Q000502:physiology*; D006801:Humans; D007302:Insect Repellents / Q000008:administration & dosage* / Q000494:pharmacology*; D010919:Placebos / Q000008:administration & dosage; D016896:Treatment Outcome; D055815:Young Adult
D007302:Insect Repellents; D010919:Placebos