Low-dose radiation suppresses Pokemon expression under hypoxic conditions.
Our previous data demonstrated that CoCl2-induced hypoxia controls endoplasmic reticulum (ER) stress-associated and other intracellular factors. One of them, the transcription factor Pokemon, was differentially regulated by low-dose radiation (LDR). There are limited data regarding how this transcription factor is involved in expression of the unfolded protein response (UPR) under hypoxic conditions. The purpose of this study was to obtain clues on how Pokemon is involved in the UPR. Pokemon was selected as a differentially expressed gene under hypoxic conditions; however, its regulation was clearly repressed by LDR. It was also demonstrated that both expression of ER chaperones and ER stress sensors were affected by hypoxic conditions, and the same results were obtained when cells in which Pokemon was up- or down-regulated were used. The current state of UPR and LDR research associated with the Pokemon pathway offers an important opportunity to understand the oncogenesis, senescence, and differentiation of cells, as well as to facilitate introduction of new therapeutic radiopharmaceuticals.
Seung-Whan
SW
Kim
Kweon
K
Yu
Kee-Sun
KS
Shin
Kisang
K
Kwon
Tae-Sik
TS
Hwang
O-Yu
OY
Kwon
D000818:Animals; D001483:Base Sequence; D015687:Cell Hypoxia; D017931:DNA Primers; D004307:Dose-Response Relationship, Radiation; D016716:PC12 Cells; D051381:Rats; D012097:Repressor Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D056811:Unfolded Protein Response
D017931:DNA Primers; D012097:Repressor Proteins; C121495:Zbtb7a protein, rat