Reporter-triggered isothermal exponential amplification strategy in ultrasensitive homogeneous label-free electrochemical nucleic acid biosensing.
A simple and novel reporter-triggered isothermal exponential amplification reaction (R-EXPAR) integrated with a miniaturized electrochemical device was developed, which achieved excellent improvement (five orders of magnitude) of sensitivity toward reporter, G-quadruplex. This R-EXPAR strategy has been successfully implemented to construct a homogeneous label-free electrochemical sensor for ultrasensitive DNA detection.
Beijing National Laboratory for Molecular Sciences (BNLMS), MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry, Peking University, Beijing 100871, China. zhouyl@pku.edu.cn zxx@pku.edu.cn.
Ji
J
Nie
De-Wen
DW
Zhang
Fang-Ting
FT
Zhang
Fang
F
Yuan
Ying-Lin
YL
Zhou
Xin-Xiang
XX
Zhang
D015374:Biosensing Techniques; D004247:DNA / Q000032:analysis*; D055664:Electrochemical Techniques; D054856:G-Quadruplexes; D006801:Humans; D021141:Nucleic Acid Amplification Techniques; D013696:Temperature
D004247:DNA