Bioethanol production from hemicellulose rich Populus nigra involving recombinant hemicellulases from Clostridium thermocellum.
Bioethanol was produced from poplar leafy biomass rich in hemicelluloses content involving recombinant Clostridium thermocellum hemicellulases and pentose sugar utilizing Candida shehatae. FT-IR analysis revealed effective AFEX pretreatment of poplar leaves. Repetitive batch strategy yielded ∼1.5-fold rise in cell biomass and specific activity of both, acetylxylanesterase (Axe) and GH43 hemicellulase. TLC and HPAEC exhibited xylose and arabinose release from hydrolyzed biomass. SSF trial with 1% (wv(-1)) pretreated poplar and mixed enzymes showed ∼1.5-fold higher ethanol titre as compared with SHF. The shake flask SSF with 5% (wv(-1)) pretreated poplar furnished 4.56 and 5.43gL(-1) ethanol with Axe and mixed enzymes, respectively. Whereas, bioreactor scale-up exhibited ∼1.25-fold increase in ethanol titres (5.68, 6.75gL(-1)) as compared with shake flask with an yield of 0.295 (gg(-1)) and 0.351 (gg(-1)), respectively with Axe and mixed enzymes.
Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India.
Ashutosh
A
Gupta
Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India.
Saprativ P
SP
Das
Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India.
Arabinda
A
Ghosh
Department of Biotechnology, National Institute of Technology Durgapur, Durgapur 713209, West Bengal, India.
Rajan
R
Choudhary
Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India.
Debasish
D
Das
Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India. Electronic address: arungoyl@iitg.ernet.in.
Arun
A
Goyal
D000641:Ammonia / Q000737:chemistry; D061249:Batch Cell Culture Techniques; D056804:Biofuels; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology; D001709:Biotechnology / Q000379:methods*; D002482:Cellulose; D002852:Chromatography, Ion Exchange; D002855:Chromatography, Thin Layer; D048013:Acetivibrio thermocellus / Q000201:enzymology*; D004926:Escherichia coli / Q000201:enzymology; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006868:Hydrolysis; D008031:Lignin; D018515:Plant Leaves / Q000378:metabolism / Q000648:ultrastructure; D011134:Polysaccharides / Q000378:metabolism*; D032107:Populus / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D012015:Reference Standards; D017550:Spectroscopy, Fourier Transform Infrared
D056804:Biofuels; D011134:Polysaccharides; D011994:Recombinant Proteins; D000431:Ethanol; D000641:Ammonia; C007916:hemicellulose; D002482:Cellulose; D008031:Lignin; D006026:Glycoside Hydrolases; C023305:hemicellulase
AFEX; Acetylxylanesterase (Axe); Bioreactor; GH43 hemicellulase; Poplar