Peptidases from latex of Carica candamarcensis upregulate COX-2 and IL-1 mRNA transcripts against Salmonella enterica ser. Typhimurium-mediated inflammation.
The immunomodulatory properties of a mixture of cysteine peptidases (P1G10) obtained from the fruit lattice of Carica candamarcensis were investigated. P1G10 was obtained from fresh latex samples by chromatography in a Sephadex column and initially administered to Swiss mice (n = 5; 1 or 10 mg/kg) via i.p. After 30 min, the mice were injected with carrageenan (0.5 mg/mouse) or heat-killed S. Typhimurium (10(7) CFU/mL; 100°C/30 min) into the peritoneal cavity. Afterwards, two animal groups were i.p. administered with P1G10 (n = 6; 1, 5, or 10 mg/Kg) or PBS 24 hours prior to challenge with live S. Typhimurium (10(7) CFU/mL). P1G10 stimulated the proliferation of circulating neutrophils and lymphocytes, 6 h after injection of carrageenan or heat-killed bacteria, respectively. Furthermore, survival after infection was dose-dependent and reached 60% of the animal group. On the other hand, control mice died 1-3 days after infection. The examination of mRNA transcripts in liver cells 24 h after infection confirmed fold variation increases of 5.8 and 4.8 times on average for IL-1 and COX-2, respectively, in P1G10 pretreated mice but not for TNF-α, IL-10, γ-IFN and iNOS, for which the results were comparable to untreated animals. These data are discussed in light of previous reports.
Departamento de Biologia, Universidade Federal Rural de Pernambuco, 52171-900 Recife, PE, Brazil.
Maria Taciana
MT
Ralph
Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, 60020-181 Fortaleza, CE, Brazil.
Ayrles Fernanda Brandão
AF
Silva
Departamento de Biologia, Universidade Federal Rural de Pernambuco, 52171-900 Recife, PE, Brazil.
Dayane Laíse
DL
da Silva
Departamento de Biologia, Universidade Federal Rural de Pernambuco, 52171-900 Recife, PE, Brazil.
Danielle Cristina Oliveira
DC
do Nascimento
Departamento de Morfologia e Fisiologia Animal, Universidade Federal Rural de Pernambuco, Recife, PE, Brazil.
Diogo Manoel Farias
DM
da Silva
Departamento de Morfologia e Fisiologia Animal, Universidade Federal Rural de Pernambuco, Recife, PE, Brazil.
Manoel A
MA
Gomes-Filho
Departamento de Biologia, Universidade Federal Rural de Pernambuco, 52171-900 Recife, PE, Brazil.
Paulo Roberto Eleutério
PR
Souza
Departamento de Morfologia e Fisiologia Animal, Universidade Federal Rural de Pernambuco, Recife, PE, Brazil.
Joaquim
J
Evêncio-Neto
Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, 60020-181 Fortaleza, CE, Brazil.
Márcio Viana
MV
Ramos
Departamento de Biologia, Facultad de Ciencia, Universidade de La Serena, La Serena, Chile.
Carlos Edmundo
CE
Salas
Departamento de Biologia, Universidade Federal Rural de Pernambuco, 52171-900 Recife, PE, Brazil ; Rua Dom Manoel de Medeiros, s/n, Departamento de Biologia, Laboratório de Microbiologia e Imunologia (LAMIM), Universidade Federal Rural de Pernambuco, Campus Dois Irmãos, CEP, 52171-900 Recife, PE, Brazil.
José Vitor
JV
Lima-Filho
D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry; D029441:Carica / Q000201:enzymology*; D049109:Cell Proliferation; D051546:Cyclooxygenase 2 / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D007249:Inflammation / Q000378:metabolism* / Q000382:microbiology; D007375:Interleukin-1 / Q000378:metabolism*; D007840:Latex; D008099:Liver / Q000201:enzymology; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D010447:Peptide Hydrolases / Q000737:chemistry*; D012333:RNA, Messenger / Q000378:metabolism; D012481:Salmonella Infections, Animal / Q000378:metabolism*; D012486:Salmonella typhimurium; D013234:Stem Cells; D013997:Time Factors
D000890:Anti-Infective Agents; D007375:Interleukin-1; D007840:Latex; D012333:RNA, Messenger; D009569:Nitric Oxide; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2; D010447:Peptide Hydrolases