Ultrasonic-assisted extraction and antioxidant activities of polysaccharides from Camellia oleifera leaves.
Polysaccharides from Camellia oleifera leaves were extracted by ultrasonic-assisted extraction and optimized with response surface methodology. The optimal conditions of the extraction of polysaccharides were determined to be the extraction time of 1.6h, extraction temperature of 88°C and ratio of liquid to raw material of 20, which contributed to the highest yield of 3.77%. The crude polysaccharide was purified and three fractions (CLP-1, CLP-2 and CLP-3) were obtained. Their physicochemical properties were investigated with chemical method. The results indicated that the uronic acid content of CLP-2 was significantly higher than those of CLP-1 and CLP-3, and CLPs were mainly composed of glucose and galactose residue. In vitro antioxidant assay indicated that three polysaccharide fractions showed remarkable antioxidant activities in a concentration-dependent manner. Compared with CLP-1 and CLP-2, CLP-3 exhibited higher iron chelating activity. CLP-2 possessed significant higher hydroxyl radial and 2,2-diphenyl-1-picrydrazy (DPPH) radical scavenging activities than those of CLP-1 and CLP-3. Overall, the polysaccharides derived from C. oleifera leaves might be explored as potential natural antioxidant.
College of Life Sciences, Sichuan Agricultural University, Yaan, Sichuan 625014, China.
Shiling
S
Feng
College of Life Sciences, Sichuan Agricultural University, Yaan, Sichuan 625014, China.
Haoran
H
Cheng
Dazhou Institute of Agricultural Sciences, Dazhou, Sichuan 635000, China.
Liang
L
Fu
College of Life Sciences, Sichuan Agricultural University, Yaan, Sichuan 625014, China. Electronic address: dcb@sicau.edu.cn.
Chunbang
C
Ding
College of Life Sciences, Sichuan Agricultural University, Yaan, Sichuan 625014, China.
Li
L
Zhang
College of Life Sciences, Sichuan Agricultural University, Yaan, Sichuan 625014, China.
Ruiwu
R
Yang
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Sichuan 611130, China.
Yonghong
Y
Zhou
D000704:Analysis of Variance; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000737:chemistry; D028244:Camellia / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000494:pharmacology; D017665:Hydroxyl Radical / Q000737:chemistry; D007502:Iron Chelating Agents / Q000494:pharmacology; D010851:Picrates / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry*; D011134:Polysaccharides / Q000302:isolation & purification* / Q000494:pharmacology*; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D013997:Time Factors; D014465:Ultrasonics / Q000379:methods*
D000975:Antioxidants; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D007502:Iron Chelating Agents; D010851:Picrates; D011134:Polysaccharides; D017665:Hydroxyl Radical; C004931:1,1-diphenyl-2-picrylhydrazyl
Antioxidant; BBD; Camellia oleifera Abel.; Monosaccharide; Polysaccharide