Age-related changes in angiogenesis in human dermis.
Present research is aimed to examine the number of dermal blood vessels, vascular endothelial growth factor (VEGF), delta-like ligand 4(Dll4) and Jagged-1 (Jag-1) in dermal blood vessels of human from 20weeks of pregnancy to 85years old. Numbers and proliferative activity of dermal fibroblast-like cells were also examined. Blood vessels were viewed with immunohistochemical staining for von Willebrand factor or CD31. VEGF, Dll4, Jag-1, and proliferating cell nuclear antigen (PCNA) were detected immunohistochemically. Results showed that the numbers of fibroblast-like cells, PCNA positive fibroblast-like cells, von Willebrand factor positive or CD31 positive blood vessels in dermis are dramatically decreased with age. The intensity of immunohistochemical staining for VEGF or Jag-1 in blood vessels of dermis is increased from antenatal to deep old period. The degree of immunohistochemical staining of dermal blood vessels for Dll4 has gone up from 20-40weeks of pregnancy to early life period (0-20years), and further decreased below antenatal values. Age-related decrease in the number of dermal blood vessels is suggested to be due to an impairment of VEGF signaling and to be mediated by Dll4 and Jag-1. It may be supposed that diminishing in blood supply of dermis occurring with age is a cause of a decrease in the number and proliferative pool of dermal fibroblasts.
Department of Obstetrics and Gynecology, Medical School Chuvash State University, Cheboksary, Russia. Electronic address: drgunin@mail.ru.
Andrei G
AG
Gunin
Department of Obstetrics and Gynecology, Medical School Chuvash State University, Cheboksary, Russia.
Vadim V
VV
Petrov
Department of Obstetrics and Gynecology, Medical School Chuvash State University, Cheboksary, Russia.
Natalia N
NN
Golubtzova
Department of Obstetrics and Gynecology, Medical School Chuvash State University, Cheboksary, Russia.
Olga V
OV
Vasilieva
Department of Obstetrics and Gynecology, Medical School Chuvash State University, Cheboksary, Russia.
Natalia K
NK
Kornilova
D048868:Adaptor Proteins, Signal Transducing; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000378:metabolism / Q000502:physiology*; D002135:Calcium-Binding Proteins / Q000378:metabolism; D002452:Cell Count; D002648:Child; D002675:Child, Preschool; D020405:Dermis / Q000098:blood supply* / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D005260:Female; D005333:Fetus / Q000098:blood supply / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D000072100:Jagged-1 Protein; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D008875:Middle Aged; D055655:NK Cell Lectin-Like Receptor Subfamily K / Q000378:metabolism; D018919:Neovascularization, Physiologic / Q000502:physiology*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D000072099:Serrate-Jagged Proteins; D012727:Sex Characteristics; D015595:Skin Aging / Q000473:pathology / Q000502:physiology*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D014841:von Willebrand Factor / Q000378:metabolism
D048868:Adaptor Proteins, Signal Transducing; D002135:Calcium-Binding Proteins; C424126:DLL4 protein, human; D036341:Intercellular Signaling Peptides and Proteins; C000606273:JAG1 protein, human; D000072100:Jagged-1 Protein; C527085:KLRK1 protein, human; D008565:Membrane Proteins; D055655:NK Cell Lectin-Like Receptor Subfamily K; D018809:Proliferating Cell Nuclear Antigen; D000072099:Serrate-Jagged Proteins; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D014841:von Willebrand Factor
Aging; Angiogenesis; Blood vessels; CD31; Dll4; Jag-1; Skin; VEGF; Von Willebrand factor