JunB/cyclin-D1 imbalance in placental mesenchymal stromal cells derived from preeclamptic pregnancies with fetal-placental compromise.
BACKGROUND
In the present study, we characterized the expression of Activating Protein 1 (AP-1) factors, key cell cycle regulators, in primary placental mesenchymal stromal cells (PDMSCs) derived from normal and preeclamptic (PE) pregnancies with fetal-placental compromise.


METHODS
PDMSCs were isolated from control (n = 20) and preeclamptic (n = 24) placentae. AP-1 expression was determined by semi-quantitative RT-PCR (sqRT-PCR), Real Time PCR and Western Blot assay. PDMSCs were plated and JunB siRNA was performed. JunB and Cyclin-D1 expression were assessed by Real Time and Western Blot analyses.


RESULTS
JunB expression was significantly increased while Cyclin-D1 expression was significantly down-regulated in PE relative to control PDMSCs. JunB siRNA was accompanied by JunB down-regulation and increased Cyclin-D1 in normal PDMSCs.


CONCLUSIONS
We described, for the first time, AP-1 expression in PDMSCs derived from physiological and PE placentae. Importantly, we demonstrated that JunB over-expression in PE-PDMSCs affects Cyclin-D1 regulation. Our data suggest a possible contribution of these pathological placental cells to the altered cell cycle regulation typical of preeclamptic placentae.
Dept. of Surgical Sciences, University of Turin, Turin, Italy.
A M
AM
Nuzzo
Dept. of Surgical Sciences, University of Turin, Turin, Italy.
D
D
Giuffrida
Dept. of Surgical Sciences, University of Turin, Turin, Italy.
C
C
Zenerino
Dept. of Surgical Sciences, University of Turin, Turin, Italy.
A
A
Piazzese
Dept. of Surgical Sciences, University of Turin, Turin, Italy.
E
E
Olearo
Dept. of Surgical Sciences, University of Turin, Turin, Italy.
T
T
Todros
Dept. of Surgical Sciences, University of Turin, Turin, Italy. Electronic address: alessandro.rolfo@unito.it.
A
A
Rolfo
D000328:Adult; D016022:Case-Control Studies; D002453:Cell Cycle; D019938:Cyclin D1 / Q000235:genetics / Q000378:metabolism*; D005260:Female; D051921:Fos-Related Antigen-2 / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D059630:Mesenchymal Stem Cells / Q000378:metabolism* / Q000473:pathology; D010920:Placenta / Q000378:metabolism* / Q000473:pathology; D011225:Pre-Eclampsia / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D011247:Pregnancy; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics; D018808:Transcription Factor AP-1 / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D055815:Young Adult; D000099169:Fos-Related Antigen 1
D019938:Cyclin D1; D051921:Fos-Related Antigen-2; D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D018808:Transcription Factor AP-1; D014157:Transcription Factors; D000099169:Fos-Related Antigen 1; C530104:CCND1 protein, human; C497872:FOSL2 protein, human; C581727:JunB protein, human
Activating protein 1; Cyclin D1; JunB; Placenta; Placental mesenchymal stromal cells; Preeclampsia