2nd dimensional GC-MS analysis of sweat volatile organic compounds prepared by solid phase micro-extraction.
The characteristics of an individual's odor from sweat, breath and skin provide important information for criminal tracking in field of forensic science. Solid phase micro-extraction gas chromatography/mass spectrometry (SPME-GC/MS) was used to determine human sweat volatile organic compounds (VOCs) profiles. The mass spectrometric analysis (with electron impact mode) followed by 2nd dimensional separation with two different GC columns (one polar and one relatively nonpolar) connected in parallel were used to identify the 574 compounds from sweat samples. The components included alcohols, aldehydes, aliphatics/aromatics, carboxylic acids, esters, ketones, and other organic compounds (amides/amines, thio/thioesters, oxide, sulfides, nitro compounds). Of these compounds, 1-tridecanol, 1,3-bis(1,1-dimethyl ethyl)-benzene, 4,4'-(1-methylethylidene) bis-phenol and 7-acetyl-6-ethyl-1,1,4,4,-tetramethyl-tetraline were common components in all donor's sweat volatile samples. Age-related specific compounds were also detected. The results suggest that characteristic volatile profiles of human sweat emanations could provide the valuable information to forensic scientists.
Department of Crime Scene Investigation, Hyechon College, Daejeon, Korea.
Mi-Jung
MJ
Choi
Department of Oriental Medical Food and Nutrition, Semyung University, Chungbuk, Korea.
Chang-Hwan
CH
Oh
D000328:Adult; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008297:Male; D008875:Middle Aged; D009812:Odorants / Q000032:analysis*; D056910:Republic of Korea; D013542:Sweat / Q000737:chemistry*; D055549:Volatile Organic Compounds / Q000032:analysis*
D055549:Volatile Organic Compounds
GC × GC-TOFMS; solid phase micro-extraction (SPME); sweat; volatile organic compounds (VOCs)