Fast determination of 22 sulfonamides from chicken breast muscle using core-shell nanoring amino-functionalized superparamagnetic molecularly imprinted polymer followed by liquid chromatography-tandem mass spectrometry.
A novel, simple and sensitive method was developed for the simultaneous determination of 22 sulfonamides (SAs) in chicken breast muscle by using the dispersive micro-solid-phase extraction (d-μ-SPE) procedure combined with ultra-fast liquid chromatography-tandem quadrupole mass spectrometry (UFLC-MS/MS). The excellent core-shell nanoring amino-functionalized superparamagnetic molecularly imprinted polymer (CS-NR-Mag-MIP) was used as sorbent, and the main factors affecting the extraction efficiency were investigated in detail. All target compounds showed good linearities in the tested range with correlation coefficients (r) higher than 0.9980. The mean recoveries were in the range of 85.0-112.2% at low, medium and high concentration levels (0.1, 1.0 and 5.0 ng/g). The intra-day and inter-day relative standard deviations (RSDs) were lower than 6.0% and 8.9%, respectively. The limits of quantification for the 22 SAs were between 0.013 and 0.099 ng/g. The developed d-μ-SPE-UFLC-MS/MS method had been successfully applied to the chicken breast muscle samples for food-safety risk monitoring in Zhejiang Province, China. The results showed sulfamethazine, sulfamethoxazole and sulfaquinoxaline in five out of three hundred collected samples were detected with concentrations of 0.418-2.28, 16.4 and 2.93 ng/g, respectively. It was confirmed that the CS-NR-Mag-MIP was a kind of highly effective d-μ-SPE materials used for the SAs analyses.
Zhejiang Provincial Key Laboratory of Health Risk Appraisal for Trace Toxic Chemicals, Ningbo Municipal Center for Disease Control and Prevention, Ningbo, Zhejiang 315010, China; Ningbo Key Laboratory of Poison Research and Control, Ningbo Municipal Center for Disease Control and Prevention, Ningbo, Zhejiang 315010, China.
Yong-Gang
YG
Zhao
Medical School, Ningbo University, Ningbo, Zhejiang 315211, China.
Li-Xin
LX
Zhou
Zhejiang Provincial Key Laboratory of Health Risk Appraisal for Trace Toxic Chemicals, Ningbo Municipal Center for Disease Control and Prevention, Ningbo, Zhejiang 315010, China; Ningbo Key Laboratory of Poison Research and Control, Ningbo Municipal Center for Disease Control and Prevention, Ningbo, Zhejiang 315010, China.
Sheng-Dong
SD
Pan
School of Marine Sciences, Ningbo University, Ningbo, Zhejiang 315211, China.
Ping-Ping
PP
Zhan
Zhejiang Provincial Key Laboratory of Health Risk Appraisal for Trace Toxic Chemicals, Ningbo Municipal Center for Disease Control and Prevention, Ningbo, Zhejiang 315010, China; Ningbo Key Laboratory of Poison Research and Control, Ningbo Municipal Center for Disease Control and Prevention, Ningbo, Zhejiang 315010, China.
Xiao-Hong
XH
Chen
Zhejiang Provincial Key Laboratory of Health Risk Appraisal for Trace Toxic Chemicals, Ningbo Municipal Center for Disease Control and Prevention, Ningbo, Zhejiang 315010, China; Ningbo Key Laboratory of Poison Research and Control, Ningbo Municipal Center for Disease Control and Prevention, Ningbo, Zhejiang 315010, China. Electronic address: jmcjc@163.com.
Mi-Cong
MC
Jin
D000818:Animals; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005290:Ferric Compounds / Q000737:chemistry*; D059627:Liquid Phase Microextraction / Q000379:methods; D058185:Magnetite Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D008460:Meat / Q000032:analysis*; D053768:Metal Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D054802:Molecular Imprinting / Q000379:methods*; D018482:Muscle, Skeletal / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D013449:Sulfonamides / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013997:Time Factors
D005290:Ferric Compounds; D058185:Magnetite Nanoparticles; D011108:Polymers; D013449:Sulfonamides; C000499:ferric oxide
Chicken breast muscle; Core–shell nanoring amino-functionalized superparamagnetic molecularly imprinted polymer (CS-NR-Mag-MIP); Dispersive micro-solid-phase extraction (d-μ-SPE); Matrix effect; Sulfonamides; Ultra-fast liquid chromatography-tandem quadrupole mass spectrometry (UFLC-MS/MS)