miR-219 inhibits the proliferation, migration and invasion of medulloblastoma cells by targeting CD164.
It is known that microRNA-219 (miR-219) expression is downregulated in medulloblastoma. In the present study, we investigated the expression, targets and functional effects of miR-219 in D283-MED medulloblastoma cells. We first demonstrated that miR-219 not only inhibits proliferation, but also suppresses the invasion and migration of D283-MED cells. Moreover, the knockdown of miR-219 promoted the proliferation, migration and invasion of the D283-MED cells. Secondly, we predicted that miR-219 targets the 3' untranslated region (3'UTR) of CD164 and orthodenticle homeobox 2 (OTX2) and then confirmed that it significantly downregulated the protein expression of CD164 and OTX2 in D283-MED cells. Finally, we demonstrated that the proliferation, invasion and migration of D283-MED cells were promoted by theectopic expression of CD164. These results indicate that miR-219 suppresses the proliferation, migration and invasion of medulloblastoma cells by targeting CD164. The results also suggest that miR-219 may serve as a potential therapeutic agent for medulloblastoma.
Department of Psychiatry, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China.
Jia-Ang
JA
Shi
Tianyou Hospital Affiliated to Wuhan University of Science and Technology, Wuhan, Hubei 430081, P.R. China.
Da-Lin
DL
Lu
Medical College, Nanchang University, Jiangxi 330000, P.R. China.
Xuan
X
Huang
Tianyou Hospital Affiliated to Wuhan University of Science and Technology, Wuhan, Hubei 430081, P.R. China.
Wei
W
Tan
D020413:3' Untranslated Regions; D001483:Base Sequence; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D051930:Endolyn / Q000235:genetics / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008527:Medulloblastoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D051857:Otx Transcription Factors / Q000235:genetics* / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction
D020413:3' Untranslated Regions; C497898:CD164 protein, human; D051930:Endolyn; C578248:MIRN219 microRNA, human; D035683:MicroRNAs; C497659:OTX2 protein, human; D051857:Otx Transcription Factors; D034741:RNA, Small Interfering