[Comparative study of antitumor activity of cantharidin and cantharis peptides].
OBJECTIVE
To prepare cantharidin and cantharidin peptides from Mylabris and compare their antitumor activity.


METHODS
Cantharis peptides was prepared by bionic enzymolysis approach and cantharidin was prepared by alkaline water supersonic extraction. The inhibitory effects of both compounds on BEL-7402 cells proliferation of human liver cancer were tested by Prestoblue method. The influence of both compounds on the grown of tumor, thymus, spleen of S180 tumor-bearing mice were detected.


RESULTS
Cantharis peptides and cantharidin could inhibit the proliferation of BEL-7402 cells (P < 0.05) in vivo, and the inhibitory effect of cantharis peptide was 12.54% lower than that of cantharidin. At the same time, they could inhibit the grown of S180 sarcoma (P < 0.05), and the inhibitory effect of cantharidin was higher than that of cantharis peptides (5.93%). Furthermore, cantharis peptides could't inhibit the grown of thymus and spleen.


CONCLUSIONS
Both cantharis peptides and cantharidin have antineoplastic activity, but cantharidin peptides have no immunosuppression.
Xiu-Ying
XY
Liao
Song-Gui
SG
Lu
Jia-Fu
JF
Feng
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D002193:Cantharidin / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D001517:Coleoptera / Q000737:chemistry; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D008297:Male; D051379:Mice; D009368:Neoplasm Transplantation; D010455:Peptides / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D011897:Random Allocation; D012510:Sarcoma 180 / Q000188:drug therapy* / Q000473:pathology; D013154:Spleen / Q000187:drug effects
D000970:Antineoplastic Agents; D010455:Peptides; D002193:Cantharidin