Supplementation with sodium selenite and selenium-enriched microalgae biomass show varying effects on blood enzymes activities, antioxidant response, and accumulation in common barbel (Barbus barbus).
Yearling common barbel (Barbus barbus L.) were fed four purified casein-based diets for 6 weeks in outdoor cages. Besides control diet, these were supplemented with 0.3 mg kg(-1) dw selenium (Se) from sodium selenite, or 0.3 and 1.0 mg kg(-1) from Se-enriched microalgae biomass (Chlorella), a previously untested Se source for fish. Fish mortality, growth, Se accumulation in muscle and liver, and activity of selected enzymes in blood plasma, muscle, liver, and intestine were evaluated. There was no mortality, and no differences in fish growth, among groups. Se concentrations in muscle and liver, activity of alanine aminotransferase and creatine kinase in blood plasma, glutathione reductase (GR) in muscle, and GR and catalase in muscle and liver suggested that selenium from Se-enriched Chlorella is more readily accumulated and biologically active while being less toxic than sodium selenite.
Research Institute of Fish Culture and Hydrobiology, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Zátiší 728/II, 389 25 Vodňany, Czech Republic.
Antonín
A
Kouba
Research Institute of Fish Culture and Hydrobiology, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Zátiší 728/II, 389 25 Vodňany, Czech Republic.
Josef
J
Velíšek
Research Institute of Fish Culture and Hydrobiology, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Zátiší 728/II, 389 25 Vodňany, Czech Republic.
Alžběta
A
Stará
Department of Phototrophic Microorganisms, AlgaTech, Institute of Microbiology, Academy of Sciences, Opatovický mlýn, 379 81 Třeboň, Czech Republic.
Jiří
J
Masojídek
Research Institute of Fish Culture and Hydrobiology, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Zátiší 728/II, 389 25 Vodňany, Czech Republic.
Pavel
P
Kozák
D000821:Animal Feed; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D018533:Biomass; D003530:Cyprinidae / Q000254:growth & development / Q000378:metabolism; D019587:Dietary Supplements; D005979:Glutathione Peroxidase / Q000097:blood*; D008099:Liver / Q000201:enzymology* / Q000378:metabolism; D058086:Microalgae / Q000737:chemistry; D012643:Selenium / Q000008:administration & dosage*; D018038:Sodium Selenite / Q000008:administration & dosage*
D000975:Antioxidants; D005979:Glutathione Peroxidase; D012643:Selenium; D018038:Sodium Selenite