Effects of fullerene C60 on proteomic profile of Danio rerio fish embryos.
The effects of phosphatidylcholine-based phospholipid nanoparticles containing fullerene C60 on Danio rerio fish embryos were studied. Exposure of the embryos with the nanoparticles for 48 h did not lead to appreciable changes in the number of protein bands in SDS-PAGE in comparison with the control (exposure in medium with phosphatidylcholine). Mass spectrometric identification of proteins showed differences in the proteomic profiles of the samples. The content of vitellogenins changed after exposure with phosphatidylcholine-based nanoparticles with C60 fullerenes. This could indicate low toxicity of the nanoparticles towards D. rerio embryos under experimental conditions.
V. N. Orekhovich Research Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Moscow, Russia.
G P
GP
Kuznetsova
O V
OV
Larina
N A
NA
Petushkova
Yu S
YS
Kisrieva
N F
NF
Samenkova
O P
OP
Trifonova
I I
II
Karuzina
O M
OM
Ipatova
K V
KV
Zolotaryov
Yu A
YA
Romashova
A V
AV
Lisitsa
D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004337:Drug Carriers / Q000633:toxicity*; D004353:Drug Evaluation, Preclinical; D004625:Embryo, Nonmammalian / Q000187:drug effects / Q000378:metabolism*; D037741:Fullerenes / Q000633:toxicity*; D053758:Nanoparticles / Q000633:toxicity; D010713:Phosphatidylcholines / Q000633:toxicity; D020543:Proteome / Q000378:metabolism*; D015027:Zebrafish; D029961:Zebrafish Proteins / Q000378:metabolism*
D004337:Drug Carriers; D037741:Fullerenes; D010713:Phosphatidylcholines; D020543:Proteome; D029961:Zebrafish Proteins; D003577:Cytochrome P-450 Enzyme System; C069837:fullerene C60