Hoechst tagging: a modular strategy to design synthetic fluorescent probes for live-cell nucleus imaging.
We report a general strategy to create small-molecule fluorescent probes for the nucleus in living cells. Our strategy is based on the attachment of the DNA-binding Hoechst compound to a fluorophore of interest. Using this approach, simple fluorescein, BODIPY, and rhodamine dyes were readily converted to novel turn-on fluorescent nucleus-imaging probes.
Department of Bioengineering, Nagaoka University of Technology, 1603-1 Kamitomioka, Nagaoka, Niigata 940-2188, Japan. tsukiji@vos.nagaokaut.ac.jp.
Akinobu
A
Nakamura
Kazumasa
K
Takigawa
Yasutaka
Y
Kurishita
Keiko
K
Kuwata
Manabu
M
Ishida
Yasushi
Y
Shimoda
Itaru
I
Hamachi
Shinya
S
Tsukiji
D001896:Boron Compounds / Q000138:chemical synthesis / Q000737:chemistry; D002467:Cell Nucleus / Q000378:metabolism*; D002470:Cell Survival; D004247:DNA / Q000737:chemistry; D015195:Drug Design; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D057054:Molecular Imaging / Q000379:methods*; D015394:Molecular Structure; D012235:Rhodamines / Q000138:chemical synthesis / Q000737:chemistry
C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D005456:Fluorescent Dyes; D012235:Rhodamines; D004247:DNA