Hormonal regulation of pigment epithelium-derived factor (PEDF) expression in the endometrium.
Pigment epithelium-derived factor (PEDF) is highly expressed in the female reproductive system and is subjected to regulation by steroid hormones in the ovary. As the uterine endometrium exhibits morphological and functional changes in response to estrogen (E2) and progesterone (P4), we aimed at characterizing the expression of PEDF in this component of the female reproductive tract and further at exploring the hormonal regulation of its expression. We found that PEDF is expressed in human and mouse endometrium. We further showed that this expression is subjected to regulation by steroid hormones, both in vivo and in vitro, as follows: E2 decreased PEDF expression and P4 increased its levels. In human endometrial samples, PEDF levels were dynamically altered along the menstrual cycle; they were low at the proliferative and early secretory phases and significantly higher at the late secretory phase. The expression levels of PEDF were inversely correlated to that of vascular endothelial growth factor (VEGF). We also showed that PEDF receptor was expressed in the endometrium and that its stimulation reduced VEGF expression. Illustrating the pattern of PEDF expression during the menstrual cycle may contribute to our understanding of the endometrial complexity.
Department of Cell and Developmental Biology, Sackler Faculty of Medicine, Tel-Aviv University, Ramat-Aviv, Tel-Aviv 69978, Israel.
Dana
D
Chuderland
IVF and Infertility Unit, Department of Obstetrics and Gynecology, Assaf Harofeh Medical Center, Zerifin 70300, Israel; Sackler Faculty of Medicine, Tel-Aviv University, Israel.
Ido
I
Ben-Ami
IVF and Infertility Unit, Department of Obstetrics and Gynecology, Assaf Harofeh Medical Center, Zerifin 70300, Israel; Sackler Faculty of Medicine, Tel-Aviv University, Israel; Infertility Clinic Kupat Cholim Meuchedet, Sprinzak Branch, Tel-Aviv, Israel.
Shevach
S
Friedler
Department of Cell and Developmental Biology, Sackler Faculty of Medicine, Tel-Aviv University, Ramat-Aviv, Tel-Aviv 69978, Israel.
Noa
N
Hasky
Department of Cell and Developmental Biology, Sackler Faculty of Medicine, Tel-Aviv University, Ramat-Aviv, Tel-Aviv 69978, Israel.
Lihi
L
Ninio-Many
Department of Cell and Developmental Biology, Sackler Faculty of Medicine, Tel-Aviv University, Ramat-Aviv, Tel-Aviv 69978, Israel.
Keren
K
Goldberg
Department of Cell and Developmental Biology, Sackler Faculty of Medicine, Tel-Aviv University, Ramat-Aviv, Tel-Aviv 69978, Israel.
Hadas
H
Bar-Joseph
Department of Cell and Developmental Biology, Sackler Faculty of Medicine, Tel-Aviv University, Ramat-Aviv, Tel-Aviv 69978, Israel.
Hadas
H
Grossman
Department of Cell and Developmental Biology, Sackler Faculty of Medicine, Tel-Aviv University, Ramat-Aviv, Tel-Aviv 69978, Israel. Electronic address: shalgir@post.tau.ac.il.
Ruth
R
Shalgi
D000818:Animals; D045744:Cell Line, Tumor; D004717:Endometrium / Q000378:metabolism*; D004958:Estradiol / Q000502:physiology*; D005136:Eye Proteins / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D005786:Gene Expression Regulation; D006801:Humans; D008597:Menstrual Cycle; D008813:Mice, Inbred ICR; D009414:Nerve Growth Factors / Q000235:genetics / Q000378:metabolism*; D009928:Organ Specificity; D011374:Progesterone / Q000502:physiology*; D015843:Serpins / Q000235:genetics / Q000378:metabolism*; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism; D000099174:Pigment Epithelium-Derived Factor
D004958:Estradiol; D005136:Eye Proteins; D009414:Nerve Growth Factors; D011374:Progesterone; D015843:Serpins; D042461:Vascular Endothelial Growth Factor A; D000099174:Pigment Epithelium-Derived Factor; C467484:VEGFA protein, human
Endometrium; Human; Mouse; PEDF; VEGF