In vitro anti-osteoporosis properties of diverse Korean Drynariae rhizoma phenolic extracts.
Drynariae rhizoma has been used to prevent bone loss that occurs with increasing age. However, the chemical compounds in extracts that act on bone metabolism in herbal medicine are poorly understood. This study aimed to investigate and compare the extraction efficacy of polyphenolic compounds, antioxidant activity, and in vitro anti-osteoporosis properties of water extract (DR-DW) and ethanol extract (DR-EtOH) from D. rhizoma. Total phenolics and flavonoids were better extracted with 70% EtOH, and this extraction method also resulted in higher antioxidant activity and in vitro anti-osteoporosis properties in these extracts. In particular, the contents of phloroglucinol, protocatechuic acid ethyl ester, 2-amino-3,4-dimethyl-benzoic acid, 3-(3,5-dimethyl-pyrazol-1-yl)-benzoic acid, chlorogenic acid, syringic acid, trans-ferulic acid, (-)-epigallocatechin, epigallocatechin gallate, quercetin dehydrate, luteolin and emodin in DR-EtOH were higher than those in DR-DW. These results indicated that DR-EtOH could be a good source of natural herbs with anti-osteoporosis properties.
Department of Animal Resources, Daegu University, Gyeongsan 712-714, Korea. whitenightt@hanmail.net.
Suk-Nam
SN
Kang
Department of Food Science and Biotechnology, Kangwon National University, Chuncheon 200-701, Korea. jongseoklee78@gmail.com.
Jong Seok
JS
Lee
Department of Food Science and Technology, Institute of Food Science, Pukyong National University, Busan 608-737, Korea. pdc327@pknu.ac.kr.
Joung-Hyun
JH
Park
Institute of Life Science, College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Korea. jaehcho@gsnu.ac.kr.
Jae-Hyeon
JH
Cho
Department of Animal Resource and Science, Dankook University, Choongnam 330-714, Korea. atom1965@hanmail.net.
Jae-Hong
JH
Park
Department of Animal Resource Technology, Gyeongnam National University of Science and Technology, Jinju 660-758, Korea. chotwo2@gntech.ac.kr.
Kwang-Keun
KK
Cho
Department of Food Science and Biotechnology, Kangwon National University, Chuncheon 200-701, Korea. loh99@kangwon.ac.kr.
Ok-Hwan
OH
Lee
Department of Animal Resource Technology, Gyeongnam National University of Science and Technology, Jinju 660-758, Korea. iskim@gntech.ac.kr.
Il-Suk
IS
Kim
D000818:Animals; D000975:Antioxidants / Q000032:analysis / Q000494:pharmacology; D002392:Catechin / Q000031:analogs & derivatives / Q000032:analysis / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D002726:Chlorogenic Acid / Q000032:analysis / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D003373:Coumaric Acids / Q000032:analysis / Q000494:pharmacology; D004642:Emodin / Q000032:analysis / Q000494:pharmacology; D062385:Hydroxybenzoates / Q000032:analysis / Q000494:pharmacology; D047311:Luteolin / Q000032:analysis / Q000494:pharmacology; D051379:Mice; D010024:Osteoporosis / Q000188:drug therapy*; D010696:Phloroglucinol / Q000032:analysis / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry; D029621:Polypodiaceae / Q000737:chemistry*; D011794:Quercetin / Q000032:analysis / Q000494:pharmacology; D056910:Republic of Korea
D000975:Antioxidants; D003373:Coumaric Acids; D062385:Hydroxybenzoates; D010936:Plant Extracts; D002726:Chlorogenic Acid; C009091:protocatechuic acid; D002392:Catechin; D011794:Quercetin; C004999:ferulic acid; C045651:epigallocatechin gallate; D010696:Phloroglucinol; C057580:gallocatechol; D004642:Emodin; D047311:Luteolin