Trophoblast specialisations during pregnancy in the tammar wallaby, Macropus eugenii: a morphological and lectin histochemical study.
BACKGROUND
The tammar wallaby has a short gestation (26.5 days) and vascular modifications to expedite transport during that brief pregnancy. Here we examine trophoblast structural attributes that would facilitate materno-fetal exchange.


METHODS
Four specimens of Macropus eugenii between days 23 and 26 gestation were examined using electron microscopy and 24 lectins to characterise glycosylated secretions and their internalisation.


RESULTS
Two trophoblast phenotypes were found, flattened cells generally in contact with the underlying uterine epithelium and giant cells associated with histiotrophe. The latter appeared to penetrate uterine clefts, occasionally detach and become necrotic. Lectin histochemistry and ultrastructure indicated the presence of many lysosomes and residual bodies especially in trophoblast giant cells; these contained glycans, mainly apically, which were also detected in secretions and cell debris. Trophoblast basal membranes bore extensive filopodia. Giant cells were less common in vascular trilaminar areas and here the trophoblast barrier became thinner near term.


CONCLUSIONS
Loss of Maackia amurensis agglutinin binding suggested cleavage of terminal sialic acid residues as an early post-internalisation event in the trophoblast. Lectin staining indicated degradation occurred in an apical-basal direction, and the heavily glycosylated basal membrane appeared specialised for transport out of the cell.


CONCLUSIONS
Granules seen ultrastructurally and histochemically, particularly in giant trophoblast cells of the bilaminar area, suggest that internalised histiotrophe is broken down here and nutrients transferred to the embryo via the specialised basal plasma membrane. The trilaminar vascular area contained mostly flattened trophoblast cells, supporting the suggestion that gaseous exchange is its primary function.
Maternal and Fetal Health Research Group, University of Manchester, Manchester Academic Health Sciences Centre, St Mary's Hospital, Oxford Road, Manchester M13 9WL, UK. Electronic address: carolyn.jones@manchester.ac.uk.
C J P
CJ
Jones
Cambridge Advanced Imaging Centre, University of Cambridge, Cambridge CB2 3DY, UK.
J N
JN
Skepper
Department of Zoology, University of Melbourne, Victoria 3010, Australia.
M B
MB
Renfree
Maternal and Fetal Health Research Group, University of Manchester, Manchester Academic Health Sciences Centre, St Mary's Hospital, Oxford Road, Manchester M13 9WL, UK.
J D
JD
Aplin
D000818:Animals; D002454:Cell Differentiation; D005260:Female; D015726:Giant Cells / Q000166:cytology / Q000378:metabolism; D006651:Histocytochemistry; D037102:Lectins / Q000378:metabolism; D007614:Macropodidae / Q000033:anatomy & histology* / Q000378:metabolism*; D008431:Maternal-Fetal Exchange; D046529:Microscopy, Electron, Transmission; D010929:Placentation; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000378:metabolism*; D014327:Trophoblasts / Q000166:cytology* / Q000378:metabolism*; D014599:Uterus / Q000033:anatomy & histology / Q000378:metabolism
D037102:Lectins
Lectins; Marsupial; Trophoblast; Ultrastructure; Wallaby