Role of Bacopa monnieri in the temporal regulation of oxidative stress in clock mutant (cryb) of Drosophila melanogaster.
Accruing evidences imply that circadian organization of biochemical, endocrinological, cellular and physiological processes contribute to wellness of organisms and in the development of pathologies such as malignancy, sleep and endocrine disorders. Oxidative stress is known to mediate a number of diseases and it is notable to comprehend the orchestration of circadian clock of a model organism of circadian biology, Drosophila melanogaster, under oxidative stress. We investigated the nexus between circadian clock and oxidative stress susceptibility by exposing D. melanogaster to hydrogen peroxide (H2O2) or rotenone; the reversibility of rhythms following exposure to Bacopa monnieri extract (ayurvedic medicine rich in antioxidants) was also investigated. Abolishment of 24h rhythms in physiological response (negative geotaxis), oxidative stress markers (protein carbonyl and thiobarbituric acid reactive substances) and antioxidants (superoxide dismutase, catalase, glutathione-S-transferase and reduced glutathione) were observed under oxidative stress. Furthermore, abolishment of per mRNA rhythm in H2O2 treated wild type flies and augmented susceptibility to oxidative stress in clock mutant (cry(b)) flies connotes the role of circadian clock in reactive oxygen species (ROS) homeostasis. Significant reversibility of rhythms was noted following B. monnieri treatment in wild type flies than cry(b) flies. Our experimental approach revealed a relationship involving oxidative stress and circadian clock in fruit fly and the utility of Drosophila model in screening putative antioxidative phytomedicines prior to their use in mammalian systems.
Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Chidambaram 608 002, Tamil Nadu, India. Electronic address: psub@rediffmail.com.
Perumal
P
Subramanian
Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Chidambaram 608 002, Tamil Nadu, India.
Vinoth
V
Prasanna
University of Malaya Centre for Proteomics Research (UMCPR), Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia; Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Jaime Jacqueline
JJ
Jayapalan
University of Malaya Centre for Proteomics Research (UMCPR), Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia; Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Puteri Shafinaz
PS
Abdul Rahman
University of Malaya Centre for Proteomics Research (UMCPR), Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia; Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Onn Haji
OH
Hashim
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D034681:Bacopa / Q000737:chemistry*; D002940:Circadian Rhythm / Q000235:genetics / Q000502:physiology*; D004331:Drosophila melanogaster / Q000235:genetics* / Q000502:physiology*; D006861:Hydrogen Peroxide / Q000378:metabolism; D008515:Medicine, Ayurvedic; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D050050:Protein Carbonylation / Q000187:drug effects; D017382:Reactive Oxygen Species; D012402:Rotenone / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D000975:Antioxidants; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D017392:Thiobarbituric Acid Reactive Substances; D012402:Rotenone; D006861:Hydrogen Peroxide
Antioxidant; Bacopa monnieri; Circadian; Cryptochrome; Drosophila