Downregulation of glutathione peroxidase 3 is associated with lymph node metastasis and prognosis in cervical cancer.
Glutathione peroxidase 3 (GPX3) is a member of the glutathione peroxidase family of selenoproteins and is one of the key defensive enzymes against oxidative damages to host cells. Downregulation of GPX3 due to its promoter hypermethylation has been documented in several different types of cancer, indicating that GPX3 functions as a possible tumor suppressor. In the present study, we showed that GPX3 is also significantly downregulated in cervical cancer tissues compared to normal cervical tissues by qRT-PCR analyses and immunohistostainings. GPX3 expression was significantly related to lymph node metastasis and prognosis in cervical cancer patients. Treatment of cervical cancer cells with 5-aza-2'-deoxycytidine restored the expression of GPX3 and methylation-specific PCR (MSP) confirmed the CpG methylation of the GPX3 gene. Our results indicate that promoter methylation is one of the major causes of GPX3 downregulation in cervical cancer and GPX3 could serve as a predictive biomarker for lymph node metastasis and prognosis of cervical cancer.
Department of Pathology, Yanbian University Hospital, Yanji, Jilin, P.R. China.
Xianglan
X
Zhang
Department of Dermatology, Yanbian University Hospital, Yanji, Jilin, P.R. China.
Zhenlong
Z
Zheng
Department of Gynecology, Maternal and Child Health Hospital, Dalian, Liaoning, P.R. China.
Shen
S
Yingji
Department of Gynecology, Gangnam Severance Hospital, Yonsei University College of Medicine, Seoul, Republic of Korea.
Hyeyeon
H
Kim
Department of Pathology, Yanbian University Hospital, Yanji, Jilin, P.R. China.
Renshun
R
Jin
Department of Anesthesia and Pain, Yanbian University Hospital, Yanji, Jilin, P.R. China.
Li
L
Renshu
Oral Cancer Research Institute, College of Dentistry, Yonsei University, Seoul, Republic of Korea.
Doo Young
DY
Lee
Department of Dermatology, Yonsei University College of Medicine, Seoul, Republic of Korea.
Mi Ryung
MR
Roh
National Creative Research Initiatives Center for Inflammatory Response Modulation, Yonsei University, Seoul, Republic of Korea.
Sanghwa
S
Yang
D001374:Azacitidine / Q000008:administration & dosage / Q000031:analogs & derivatives; D018899:CpG Islands / Q000187:drug effects; D019175:DNA Methylation / Q000187:drug effects; D000077209:Decitabine; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D005979:Glutathione Peroxidase / Q000096:biosynthesis* / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D008207:Lymphatic Metastasis / Q000235:genetics* / Q000473:pathology; D011379:Prognosis; D011401:Promoter Regions, Genetic / Q000187:drug effects; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000473:pathology
D000077209:Decitabine; C486484:GPX3 protein, human; D005979:Glutathione Peroxidase; D001374:Azacitidine