Direct electrochemistry of glucose oxidase and glucose biosensing on a hydroxyl fullerenes modified glassy carbon electrode.
Direct electrochemistry of glucose oxidase (GOD) was achieved when GOD-hydroxyl fullerenes (HFs) nano-complex was immobilized on a glassy carbon (GC) electrode and protected with a chitosan (Chit) membrane. The ultraviolet-visible absorption spectrometry (UV-vis), transmission electron microscopy (TEM), and circular dichroism spectropolarimeter (CD) methods were utilized for additional characterization of the GOD, GOD-HFs and Chit/GOD-HFs. Chit/HFs may preserve the secondary structure and catalytic properties of GOD. The cyclic voltammograms (CVs) of the modified GC electrode showed a pair of well-defined quasi-reversible redox peaks with the formal potential (E°') of 353 ± 2 mV versus Ag/AgCl at a scan rate of 0.05 V/s. The heterogeneous electron transfer constant (ks) was calculated to be 2.7 ± 0.2s(-1). The modified electrode response to glucose was linear in the concentrations ranging from 0.05 to 1.0mM, with a detection limit of 5 ± 1 μM. The apparent Michaelis-Menten constant (Km(app)) was 694 ± 8 μM. Thus, the modified electrode could be applied as a third generation biosensor for glucose with high sensitivity, selectivity and low detection limit.
School of Life Sciences, Henan University, Kaifeng 475000, China.
Yun-Fei
YF
Gao
School of Life Sciences, Henan University, Kaifeng 475000, China.
Tian
T
Yang
School of Life Sciences, Henan University, Kaifeng 475000, China.
Xiao-Lu
XL
Yang
School of Life Sciences, Henan University, Kaifeng 475000, China.
Yu-Shuai
YS
Zhang
School of Life Sciences, Henan University, Kaifeng 475000, China.
Bao-Lin
BL
Xiao
School of Life Sciences, Henan University, Kaifeng 475000, China; Institute of Biotechnology, Henan University, Kaifeng 475000, China. Electronic address: hongjun@henu.edu.cn.
Jun
J
Hong
Department of Ophthalmology and Visual Sciences and Biomidical Engineering, University of Wisconsin, School of Medicine and Public Health, Madison, WI, USA.
Nader
N
Sheibani
Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.
Hedayatollah
H
Ghourchian
School of Art, Henan University, Kaifeng 475000, China.
Tao
T
Hong
Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran. Electronic address: moosavi@ibb.ut.ac.ir.
Ali Akbar
AA
Moosavi-Movahedi
D015374:Biosensing Techniques / Q000295:instrumentation*; D002244:Carbon / Q000737:chemistry*; D003217:Conductometry / Q000295:instrumentation*; D004566:Electrodes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D037741:Fullerenes / Q000737:chemistry*; D005898:Glass / Q000737:chemistry; D005947:Glucose / Q000032:analysis* / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry*; D006878:Hydroxides
D037741:Fullerenes; D006878:Hydroxides; D002244:Carbon; C031356:hydroxide ion; D005949:Glucose Oxidase; D005947:Glucose
Direct electrochemistry; Glucose oxidase; Hydroxyl fullerenes; Nano-complex