Microfluidics for single-cell genetic analysis.
The ability to correlate single-cell genetic information to cellular phenotypes will provide the kind of detailed insight into human physiology and disease pathways that is not possible to infer from bulk cell analysis. Microfluidic technologies are attractive for single-cell manipulation due to precise handling and low risk of contamination. Additionally, microfluidic single-cell techniques can allow for high-throughput and detailed genetic analyses that increase accuracy and decrease reagent cost compared to bulk techniques. Incorporating these microfluidic platforms into research and clinical laboratory workflows can fill an unmet need in biology, delivering the highly accurate, highly informative data necessary to develop new therapies and monitor patient outcomes. In this perspective, we describe the current and potential future uses of microfluidics at all stages of single-cell genetic analysis, including cell enrichment and capture, single-cell compartmentalization and manipulation, and detection and analyses.
Department of Chemistry, University of Washington, Seattle, WA, USA. chiu@chem.washington.edu.
A M
AM
Thompson
A L
AL
Paguirigan
J E
JE
Kreutz
J P
JP
Radich
D T
DT
Chiu
D015894:Genome, Human; D006801:Humans; D044085:Microfluidics / Q000379:methods*; D016133:Polymerase Chain Reaction; D017421:Sequence Analysis / Q000379:methods*; D059010:Single-Cell Analysis