Use of mixed culture bacteria for photofermentive hydrogen of dark fermentation effluent.
Hydrogen production (HP) from dark fermentation effluent of starch wastewater via vertical tubular photo-bioreactor was investigated. The reactor was inoculated with mixed culture of bacteria and operated at light intensity of 190 W/m(2). Hydraulic retention time (HRT) and organic loading rate (OLR) was varied between 0.9 to 4.0 h and 3.2 to 16 g COD/l.d., respectively. Increasing the HRT from 0.9 to 2.5 h, significantly (P<0.05) increased HP from 1±0.04 to 3.05±0.19 l/d, respectively. However, minimal increase in HP occurred when increasing the HRT up to 4.0 h. The HP remained unaffected when increasing the OLR from 3.2 to 6.4 g COD/l.d. Further increase in the OLR up to 8.2 and 16 g COD/l.d., resulted in a drop in HP i.e. 0.96 and 0.19 l/d, respectively. Microbial community analysis of the reactor samples showed the presence and dominance of hydrogen producing purple non-sulfur phototrophic (PNS) bacterium, Rhodopseudomonas palustris in the reactor.
Egypt-Japan University of Science and Technology (E-Just), Environmental Engineering Department, P.O. Box 179, New Borg El Arab City, 21934 Alexandria, Egypt.
Ahmed
A
Tawfik
Egypt-Japan University of Science and Technology (E-Just), Environmental Engineering Department, P.O. Box 179, New Borg El Arab City, 21934 Alexandria, Egypt.
Haitham
H
El-Bery
Institute for Water and Wastewater Technology, Durban University of Technology, P.O. Box 1334, Durban 4000, South Africa.
Sheena
S
Kumari
Institute for Water and Wastewater Technology, Durban University of Technology, P.O. Box 1334, Durban 4000, South Africa. Electronic address: faizalb@dut.ac.za.
Faizal
F
Bux
D064751:Ammonium Compounds / Q000032:analysis; D001419:Bacteria / Q000378:metabolism*; D057919:Biological Oxygen Demand Analysis; D002264:Carboxylic Acids / Q000032:analysis; D003624:Darkness; D058645:Denaturing Gradient Gel Electrophoresis; D005232:Fatty Acids, Volatile / Q000032:analysis; D005285:Fermentation; D006859:Hydrogen / Q000378:metabolism*; D058433:Photobioreactors / Q000382:microbiology; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D013997:Time Factors; D014865:Waste Disposal, Fluid
D064751:Ammonium Compounds; D002264:Carboxylic Acids; D005232:Fatty Acids, Volatile; D006859:Hydrogen
Bio-hydrogen; HRT; Microbial analysis; Photo-fermentation; Vertical tubular photo-bioreactor