Temperature-responsive peptide-mimetic coating based on poly(N-methacryloyl-l-leucine): properties, protein adsorption and cell growth.
Poly(N-methacryloyl-l-leucine) (PNML) coatings were successfully fabricated via polymerization from peroxide initiator grafted to premodified glass substrate. Chemical composition and thickness of PNML coatings were determined using time of flight-secondary ion mass spectrometry (TOF- SIMS) and ellipsometry, respectively. PNML coatings exhibit thermal response of the wettability, between 4 and 28°C, which indicates a transition between hydrated loose coils and hydrophobic collapsed chains. Morphology of the PNML coating was observed with the AFM, transforming with increasing temperature from initially relatively smooth surface to rough and more structured surface. Protein adsorption observed by fluorescence microscopy for model proteins (bovine serum albumin and lentil lectin labeled with fluorescein isothiocyanate) at transition from 5 to 25°C, showed high affinity of PNML coating to proteins at all investigated temperatures and pH. Thus, PNML coating have significant potential for medical and biotechnological applications as protein capture agents or functional replacements of antibodies ("plastic antibodies"). The high proliferation growth of the human embryonic kidney cell (HEK 293) onto PNML coating was demonstrated, indicating its excellent cytocompatibility.
Smoluchowski Institute of Physics, Jagiellonian University, Reymonta 4, 30-059 Kraków, Poland. Electronic address: joanna.raczkowska@uj.edu.pl.
Joanna
J
Raczkowska
"Lvivska Polytechnika" National University, S. Bandery 12, 79013 Lviv, Ukraine.
Mariya
M
Ohar
"Lvivska Polytechnika" National University, S. Bandery 12, 79013 Lviv, Ukraine. Electronic address: yrstecushun@ukr.net.
Yurij
Y
Stetsyshyn
Smoluchowski Institute of Physics, Jagiellonian University, Reymonta 4, 30-059 Kraków, Poland.
Joanna
J
Zemła
Smoluchowski Institute of Physics, Jagiellonian University, Reymonta 4, 30-059 Kraków, Poland.
Kamil
K
Awsiuk
Smoluchowski Institute of Physics, Jagiellonian University, Reymonta 4, 30-059 Kraków, Poland.
Jakub
J
Rysz
Smoluchowski Institute of Physics, Jagiellonian University, Reymonta 4, 30-059 Kraków, Poland.
Katarzyna
K
Fornal
AGH University of Science and Technology, Faculty of Physics and Applied Computer Science, al. A. Mickiewicza 30, 30-059 Kraków, Poland.
Andrzej
A
Bernasik
"Lvivska Polytechnika" National University, S. Bandery 12, 79013 Lviv, Ukraine.
Halyna
H
Ohar
Institute of Animal Biology, National Academy of Agrarian Sciences of Ukrainian, Vasyl' Stus Str. 38, 79034 Lviv, Ukraine.
Svitlana
S
Fedorova
Institute of Animal Biology, National Academy of Agrarian Sciences of Ukrainian, Vasyl' Stus Str. 38, 79034 Lviv, Ukraine.
Oksana
O
Shtapenko
"Lvivska Polytechnika" National University, S. Bandery 12, 79013 Lviv, Ukraine.
Svyatoslav
S
Polovkovych
"Lvivska Polytechnika" National University, S. Bandery 12, 79013 Lviv, Ukraine.
Volodymyr
V
Novikov
Smoluchowski Institute of Physics, Jagiellonian University, Reymonta 4, 30-059 Kraków, Poland.
Andrzej
A
Budkowski
D000818:Animals; D002417:Cattle; D049109:Cell Proliferation / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D057809:HEK293 Cells; D006801:Humans; D007930:Leucine / Q000031:analogs & derivatives* / Q000494:pharmacology; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology*; D058105:Polymerization / Q000187:drug effects; D012710:Serum Albumin, Bovine / Q000302:isolation & purification*; D018629:Spectrometry, Mass, Secondary Ion; D013696:Temperature; D014867:Water / Q000737:chemistry; D017155:Wettability / Q000187:drug effects
D020099:Coated Materials, Biocompatible; D010455:Peptides; C532401:methacryloyl-L-leucine; D014867:Water; D012710:Serum Albumin, Bovine; D007930:Leucine
Biomimetic polymer coatings; Graft polymerization; Poly(N-methacryloyl amino acids); Protein adsorption; Temperature-responsive polymer coatings; Wettability