[Effect of sodium houttuyfonate in enhancing imipenem's activity against Pseudomonas aeruginosa biofilms].
OBJECTIVE
To investigate the resistant effect of houttuyfonate sodium (SH) combined with imipenem (IMP) against Pseudomonas aeruginosa (Pa) biofilms.


METHODS
The two-fold dilution method was used to examine the minimum inhibitory concentration (MIC) of the tested drug. The crystal violet staining was applied to detect the effect of the combination of 1/2MIC, 1MIC, 2MIC of SH, single IMP, 1/2MIC of SH and IMP of various concentrations on the clearance rate of adherent bacteria, growth of biofilms and alginate production. Fluorescein diacetate (FDA)-propidium iodide (PI) doubling staining assay was employed to observe the bacterial viability and morphological changes after membrane dispersion of each drug group.


RESULTS
Sodium houttuyfonate could enhance the effect of IMP against pseudomonas aeruginosa biofilms. Particularly, the combination group with the concentration of 2MIC showed the highest effect, with P < 0.001 compared with the negative control group. The above results were proved by the bacterial viability and biofilm morphology under fluorescence microscope.


CONCLUSIONS
After being combined with imipenem, sodium houttuyfonate shows a higher effect against biofilms. It is expected that the combination of the two drugs could improve the clinical efficacy of associated infections.
Hui-Juan
HJ
Cheng
Min
M
Yang
Chang-Zhong
CZ
Wang
Chang-Feng
CF
Zhang
Chun-Yun
CY
Chu
D000473:Alkanes / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development*; D004357:Drug Synergism; D015378:Imipenem / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability / Q000187:drug effects; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000502:physiology*; D013447:Sulfites / Q000494:pharmacology*
D000473:Alkanes; D013447:Sulfites; C473296:sodium houttuyfonate; D015378:Imipenem