Impact of the hepatic branch of the vagus and Roux-en-Y gastric bypass on the hypoglycemic effect and glucagon-like peptide-1 in rats with type 2 diabetes mellitus.
BACKGROUND
The impact of the hepatic branch of the vagus and Roux-en-Y gastric bypass (RYGB) on the hypoglycemic effect and glucagon-like peptide-1 (GLP-1) in rats with type 2 diabetes mellitus (T2DM) was investigated, and interactions were preliminarily analyzed.


METHODS
A total of 45 rats with T2DM were divided into four groups: sham operation (S, n = 10), sham operation with the hepatic branch of the vagus resected (SV, n = 11), RYGB (n = 12), and RYGB without preservation of the vagus (RYGBV, n = 12). Body mass, fasting blood glucose (FBG), fasting serum insulin, and concentrations of fasting serum GLP-1 were examined in the first, second, fourth, and eighth week before and after surgery. The effects of RYGB and the hepatic branch of the vagus on GLP-1 levels in the eighth postoperative week were also analyzed.


RESULTS
RYGB caused a significant reduction in the weight of rats with T2DM (P < 0.05), improved the levels of serum GLP-1 and insulin (P < 0.05), and decreased FBG level (P < 0.05). Retention of the hepatic branch of the vagus maintained weight reduction for a longer period (P < 0.05) and increased the levels of serum GLP-1 and insulin (P < 0.05), but had no impact on FBG level (P > 0.05).


CONCLUSIONS
RYGB had better therapeutic efficacy in rats with T2DM. Care should be taken during RYGB surgery to preserve the hepatic branch of the vagus.
Department of General Surgery, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.
Nian-Cun
NC
Qiu
Department of General Surgery, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.
Qin
Q
Zhang
Department of General Surgery, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.
Xin
X
Song
Department of Obstetrics and Gynecology, Women's Hospital, School of Medicine Zhejiang University, Zhejiang 310000, China.
Miao-E
ME
Liu
Department of General Surgery, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.
Xiao-Ke
XK
Li
Department of General Surgery, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China. Electronic address: chengxiangshan@hotmail.com.
Cheng-Xiang
CX
Shan
Department of General Surgery, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China. Electronic address: qium127@163.com.
Ming
M
Qiu
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism / Q000601:surgery; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism / Q000601:surgery*; D004195:Disease Models, Animal; D015390:Gastric Bypass / Q000379:methods*; D052216:Glucagon-Like Peptide 1 / Q000097:blood*; D007003:Hypoglycemia / Q000378:metabolism; D007328:Insulin / Q000097:blood; D008099:Liver / Q000294:innervation*; D008297:Male; D009765:Obesity / Q000378:metabolism / Q000601:surgery; D011184:Postoperative Period; D017207:Rats, Sprague-Dawley; D014628:Vagotomy / Q000009:adverse effects*; D014630:Vagus Nerve / Q000033:anatomy & histology / Q000502:physiology / Q000601:surgery*; D015431:Weight Loss / Q000502:physiology
D001786:Blood Glucose; D007328:Insulin; D052216:Glucagon-Like Peptide 1
Glucagon-like peptide-1; Hepatic branch of the vagus; Obesity; Roux-en-Y gastric bypass; Type 2 diabetes