The effect of some conditions on the secretion of extracellular beta-galactosidase synthesised by Kluyveromyces fragilis 28.
To induce extracellular secretion of beta-galactosidase synthesised by Kluyveromyces fragilis 28, it was used glycine, L-asparagine, L-leucine, dimethyl fluoride, dimethyl sulfoxide, cetyldimethylethylammonium bromide, penicillin G and glycolipids from Candida antarctica. The highest increase in the secretion of beta-galactosidase was obtained in the yeast culture cultivated in the medium with polypeptone when glycine was used as the secretion inductor. The extracellular activity of beta-galactosidase reached 0.416 A.U./ml, and was 10-fold higher than the beta-galactosidase activity reported in the control group.
A
A
Demczuk
J
J
Kowalewska-Piontas
W
W
Bednarski
D002175:Candida / Q000378:metabolism; D005998:Glycine / Q000378:metabolism; D007716:Kluyveromyces / Q000201:enzymology*; D010461:Peptones / Q000378:metabolism; D001616:beta-Galactosidase / Q000096:biosynthesis*
D010461:Peptones; D001616:beta-Galactosidase; D005998:Glycine