The pea seedling mitochondrial Nε-lysine acetylome.
Posttranslational lysine acetylation is believed to occur in all taxa and to affect thousands of proteins. In contrast to the hundreds of mitochondrial proteins reported to be lysine-acetylated in non-plant species, only a handful have been reported from the plant taxa previously examined. To investigate whether this reflects a biologically significant difference or merely a peculiarity of the samples thus far examined, we immunoenriched and analyzed acetylated peptides from highly purified pea seedling mitochondria using mass spectrometry. Our results indicate that a multitude of mitochondrial proteins, involved in a variety of processes, are acetylated in pea seedlings.
Division of Biochemistry, University of Missouri, Columbia, MO 65211, USA; Interdisciplinary Plant Group, University of Missouri, Columbia, MO 65211, USA. Electronic address: clsppb@missouri.edu.
Colin L
CL
Smith-Hammond
Division of Biochemistry, University of Missouri, Columbia, MO 65211, USA; Interdisciplinary Plant Group, University of Missouri, Columbia, MO 65211, USA. Electronic address: hoyosm@missouri.edu.
Elizabeth
E
Hoyos
Division of Biochemistry, University of Missouri, Columbia, MO 65211, USA; Interdisciplinary Plant Group, University of Missouri, Columbia, MO 65211, USA; Plant Genetics Research Unit, USDA, Agricultural Research Service, University of Missouri, Columbia, MO 65211, USA. Electronic address: jan.miernyk@ars.usda.gov.
Ján A
JA
Miernyk
D000107:Acetylation; D008239:Lysine / Q000378:metabolism*; D008928:Mitochondria / Q000737:chemistry* / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000032:analysis / Q000302:isolation & purification; D018532:Pisum sativum / Q000737:chemistry* / Q000378:metabolism; D010940:Plant Proteins / Q000032:analysis* / Q000302:isolation & purification; D011499:Protein Processing, Post-Translational; D020543:Proteome / Q000032:analysis*; D036226:Seedlings / Q000737:chemistry* / Q000378:metabolism
D024101:Mitochondrial Proteins; D010940:Plant Proteins; D020543:Proteome; D008239:Lysine
Lysine acetylation; Mass spectrometry; Pisum sativum; Proteomics