[The detection of Leiden mutation using technique of enzymatic extension of allele-specific primer with double bioluminescent detection (PED-Biolum)].
The article deals with comparing technique of detection of Leiden mutation on the basis of PEXT-reaction with subsequent bioluminescent microanalysis of products with technique based on RT-PCR. The sampling for testing comprised 83 specimen of genome DNA including 35 specimens with known Leiden heterozygote mutation. The commercial kit "SNP-express-PB" (Litex) was used as a comparison test. It is demonstrated that proposed approach is a simple in its application, effective and relatively inexpensive technique of detection of Leiden one-nucleotide polymorphism in gene V of blood coagulation factor. The technique "PED-Biolum" has no differences in comparison with commercial technique RT-PCR concerning ability to detect mutant allele and matches it in parameters of economic effectiveness.
V V
VV
Krasitskaia
T N
TN
Subbotina
I A
IA
Ol'khovskiĭ
L A
LA
Frank
D000483:Alleles; D017931:DNA Primers / Q000737:chemistry / Q000235:genetics; D005165:Factor V / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008163:Luminescent Measurements / Q000379:methods*; D009154:Mutation / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide
D017931:DNA Primers; D005165:Factor V