Preparation and evaluation of a novel monolithic column containing double octadecyl chains for reverse-phase micro high performance liquid chromatography.
A novel reverse-phase monolithic stationary phase containing double long alkyl chains was prepared based on the thermally initiated co-polymerization of 3-methylacryloyl-3-oxapropyl-3-(N,N-dioctadecylcarbamoyl)-propionate (AOD) and ethylene glycol dimethacrylate (EDMA) in the presence of 2-methyl-1-propanol and 1,4-butanediol as the selected porogens. The polymerization was carefully optimized and good permeability, stability and column efficiency were observed for the final monolithic columns. The column also showed good long term stability and reproducibility. The methylene selectivity demonstrates typical reversed phase characteristics. The optimized poly (AOD-co-EDMA) monolith exhibited good selectivity for a range of non-polar test analytes such as PAHs, tocopherols and alkylphenones. A good separation of intact proteins was also observed.
Department of pharmacy and Guangdong Province Key Laboratory of Pharmacodynamic of Traditional Chinese Medicine & New Drug Research, Jinan University, Guangzhou 510632, China.
Qianqian
Q
Duan
Department of pharmacy and Guangdong Province Key Laboratory of Pharmacodynamic of Traditional Chinese Medicine & New Drug Research, Jinan University, Guangzhou 510632, China.
Chusheng
C
Liu
Department of pharmacy and Guangdong Province Key Laboratory of Pharmacodynamic of Traditional Chinese Medicine & New Drug Research, Jinan University, Guangzhou 510632, China.
Zhenghua
Z
Liu
Department of pharmacy and Guangdong Province Key Laboratory of Pharmacodynamic of Traditional Chinese Medicine & New Drug Research, Jinan University, Guangzhou 510632, China.
Zhengyin
Z
Zhou
Department of pharmacy and Guangdong Province Key Laboratory of Pharmacodynamic of Traditional Chinese Medicine & New Drug Research, Jinan University, Guangzhou 510632, China.
Weijia
W
Chen
Department of pharmacy and Guangdong Province Key Laboratory of Pharmacodynamic of Traditional Chinese Medicine & New Drug Research, Jinan University, Guangzhou 510632, China.
Qiqin
Q
Wang
Department of pharmacy and Guangdong Province Key Laboratory of Pharmacodynamic of Traditional Chinese Medicine & New Drug Research, Jinan University, Guangzhou 510632, China; Laboratory of Analytical Pharmaceutical Chemistry, Department of Pharmaceutical Sciences, University of Liege, CHU B36, Liege B-4000, Belgium.
Jacques
J
Crommen
Department of pharmacy and Guangdong Province Key Laboratory of Pharmacodynamic of Traditional Chinese Medicine & New Drug Research, Jinan University, Guangzhou 510632, China. Electronic address: jzjjackson@hotmail.com.
Zhengjin
Z
Jiang
D000818:Animals; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D056148:Chromatography, Reverse-Phase / Q000295:instrumentation*; D045304:Cytochromes c / Q000302:isolation & purification; D005026:Ethylene Glycols / Q000737:chemistry*; D008689:Methacrylates / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D009113:Muramidase / Q000302:isolation & purification; D011084:Polycyclic Aromatic Hydrocarbons / Q000302:isolation & purification; D015203:Reproducibility of Results; D012260:Ribonucleases / Q000302:isolation & purification; D024505:Tocopherols / Q000302:isolation & purification
C000592303:3-methylacryloyl-3-oxapropyl-3-(N,N-dioctadecylcarbamoyl)-propionat-co-ethylene glycol dimethacrylate; D005026:Ethylene Glycols; D008689:Methacrylates; D011084:Polycyclic Aromatic Hydrocarbons; C004919:ethylene dimethacrylate; D045304:Cytochromes c; D012260:Ribonucleases; D009113:Muramidase; D024505:Tocopherols
Double alkyl chains; Micro-HPLC; Monoliths; Reverse-phase liquid chromatography