Graphene oxide and metal-mediated base pairs based "molecular beacon" integrating with exonuclease I for fluorescence turn-on detection of biothiols.
A novel fluorescence turn-on strategy, based on the resistance of metal-mediated molecular-beacons (MBs) toward nuclease digestion and the remarkable difference in the affinity of graphene oxide (GO) with MBs and the mononucleotides, is designed for the biothiols assay. Specifically, the metal-mediated base pairs facilitate the dye labeled MBs to fold into a hairpin structure preventing the digestion by exonuclease I, and thus allow the fluorescence quenching. The competition binding by biothiols removes metal ions from the base pairs, causing the nuclease reaction, and less decrease in the fluorescence is obtained after incubating with GO due to the weak affinity of the product-mononucleotides to GO. Hg(2+)-mediated MBs were firstly designed for the biothiols detection, and glutathione (GSH) was applied as the model target. Under the optimal conditions, the approach exhibits high sensitivity to GSH with a detection limit of 1.53 nM. Ag(+)-mediated MBs based sensor was also constructed to demonstrate its versatility, and cysteine was studied as the model target. The satisfactory results in the determination of biothiols in serum demonstrate that the method possesses great potential for detecting thiols in biological fluids. This new approach is expected to promote the exploitation of metal-mediated base pairs-based biosensors in biochemical and biomedical studies.
Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan Institute of Biotechnology, and State, Key Laboratory of Virology, Wuhan University, Wuhan, 430072, P. R. China.
Xiaojing
X
Xing
Ying
Y
Zhou
Xueguo
X
Liu
Daiwen
D
Pang
Hongwu
H
Tang
D020029:Base Pairing; D005090:Exodeoxyribonucleases / Q000737:chemistry*; D005978:Glutathione / Q000032:analysis*; D006108:Graphite / Q000737:chemistry*; D057230:Limit of Detection; D010087:Oxides / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013438:Sulfhydryl Compounds / Q000032:analysis*
D010087:Oxides; D013438:Sulfhydryl Compounds; D006108:Graphite; D005090:Exodeoxyribonucleases; C028450:exodeoxyribonuclease I; D005978:Glutathione
biothiol detection; exonuclease І; graphene oxide; metal-mediated molecular-beacon