Reconstruction of abdominal wall defects using small intestinal submucosa coated with gelatin hydrogel incorporating basic fibroblast growth factor.
OBJECTIVE
To construct a new biomaterial-small intestinal submucosa coated with gelatin hydrogel incorporating basic fibroblast growth factor, and to evaluate the new biomaterials for the reconstruction of abdominal wall defects.


METHODS
Thirty six Sprague-Dawley rats were used in the animal experiments and randomly divided into three groups. The new biomaterial was constructed by combining small intestinal submucosa with gelatin hydrogel for basic fibroblast growth factor release. Abdominal wall defects were created in rats, and repaired using the new biomaterials (group B), compared with small intestinal submucosa (group S) and ULTRAPROTM mesh (group P). Six rats in each group were sacrificed at three and eight weeks postoperatively to examine the gross effects, inflammatory responses, collagen deposition and neovascularization.


RESULTS
After implantation, mild adhesion was caused in groups B and S. Group B promoted more neovascularization than group S at three weeks after implantation, and induced significantly more amount of collagen deposition and better collagen organization than groups S and P at eight weeks after implantation.


CONCLUSIONS
Small intestinal submucosa coated with gelatin hydrogel incorporating basic fibroblast growth factor could promote better regeneration and remodeling of host tissues for the reconstruction of abdominal wall defects.
Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China.
Liang
L
Wang
Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China.
Dong-ming
DM
Lai
Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China.
Bin
B
Yang
Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China.
Zhi-peng
ZP
Jiang
Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China.
Yu-chao
YC
Zhang
Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China.
Jun
J
Zhou
Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China.
Wei
W
Lai
Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China.
Shuang
S
Chen
D034861:Abdominal Wall / Q000473:pathology / Q000601:surgery*; D000818:Animals; D001672:Biocompatible Materials / Q000627:therapeutic use*; D003094:Collagen / Q000032:analysis; D016222:Fibroblast Growth Factor 2 / Q000627:therapeutic use*; D005780:Gelatin / Q000627:therapeutic use*; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000627:therapeutic use*; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000637:transplantation*; D007421:Intestine, Small; D008422:Materials Testing; D011897:Random Allocation; D017207:Rats, Sprague-Dawley; D012038:Regeneration; D015203:Reproducibility of Results; D013997:Time Factors; D000267:Tissue Adhesions; D016896:Treatment Outcome
D001672:Biocompatible Materials; D016222:Fibroblast Growth Factor 2; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D005780:Gelatin; D003094:Collagen