Significance of branching for transfection: synthesis of highly branched degradable functional poly(dimethylaminoethyl methacrylate) by vinyl oligomer combination.
A series of degradable branched PDMAEMA copolymers were investigated with the linear PDMAEMA counterpart as gene-delivery vectors. The branched PDMAEMA copolymers were synthesized by controlled radical cross-linking copolymerization based on the "vinyl oligomer combination" approach. Efficient degradation properties were observed for all of the copolymers. The degree of branching was found to have a big impact on performance in transfection when tested on different cell types. The product with the highest degree of branching and highest degree of functionality had a superior transfection profile in terms of both transfection capability and the preservation of cell viability. These branched PDMAEMA copolymers show high potential for gene-delivery applications through a combination of the simplicity of their synthesis, their low toxicity, and their high performance.
Charles Institute of Dermotology, University College Dublin, Dublin (Ireland) http://www.wenxinwang.ie; School of Materials Science and Engineering, Tianjin Universiy, Tianjin (China).
Tianyu
T
Zhao
Hong
H
Zhang
Ben
B
Newland
Ahmed
A
Aied
Dezhong
D
Zhou
Wenxin
W
Wang
D006801:Humans; D008689:Methacrylates / Q000737:chemistry*; D009757:Nylons / Q000737:chemistry*; D058105:Polymerization; D011108:Polymers / Q000138:chemical synthesis*; D011143:Polyvinyl Chloride / Q000737:chemistry*; D014162:Transfection
D008689:Methacrylates; D009757:Nylons; D011108:Polymers; C407037:poly(2-(dimethylamino)ethyl methacrylate); D011143:Polyvinyl Chloride
branched polymers; copolymerization; degradable polymers; transfection; vinyl oligomer