Natural occurrence of ochratoxin A in wolfberry fruit wine marketed in China.
Wolfberry fruit wine (WFW) is widely used as a global functional food to improve the immune system and prevent human disease. A total of 36 bottled WFWs were randomly collected in China between 2005 and 2010. Samples were analysed for the presence of ochratoxin A (OTA) using immunoaffinity column (IAC) clean-up and high-performance liquid chromatography with fluorescence detection (HPLC-FLD). Positive results were confirmed by liquid chromatography-electrospray ionisation-tandem mass spectrometry (LC-ESI-MS/MS). The limit of detection (LOD), based on a signal-to-noise ratio of 3, was 0.05 ng mL⁻¹. Recoveries ranged from 78.3% to 94.7% and relative standard deviations from 1.1% to 4.3% within the spiking range of 0.2-20 ng mL⁻¹. OTA was detected in one sample, below the maximum allowable limit as established by the European community.
a Institute of Medicinal Plant Development , Chinese Academy of Medical Sciences, Peking Union Medical College , Beijing , China.
Ying
Y
Kuang
Feng
F
Qiu
Weijun
W
Kong
Meihua
M
Yang
D053000:Analytic Sample Preparation Methods; D001230:Aspergillus / Q000378:metabolism; D002273:Carcinogens / Q000032:analysis* / Q000378:metabolism; D002681:China; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D004032:Diet / Q000208:ethnology; D005506:Food Contamination; D005513:Food Inspection / Q000379:methods*; D005638:Fruit / Q000737:chemistry* / Q000382:microbiology; D019983:Guideline Adherence; D006291:Health Policy; D006801:Humans; D007166:Immunosuppressive Agents / Q000032:analysis / Q000378:metabolism; D032305:Lycium / Q000737:chemistry* / Q000382:microbiology; D009793:Ochratoxins / Q000032:analysis* / Q000096:biosynthesis; D010407:Penicillium / Q000378:metabolism; D053719:Tandem Mass Spectrometry; D013723:Teratogens / Q000032:analysis / Q000378:metabolism; D014920:Wine / Q000032:analysis* / Q000191:economics / Q000382:microbiology / Q000592:standards
D002273:Carcinogens; D007166:Immunosuppressive Agents; D009793:Ochratoxins; D013723:Teratogens; C025589:ochratoxin A
HPLC-FLD; LC-ESI-MS/MS; ochratoxin A; wolfberry fruit wine