The major cockroach allergen Bla g 4 binds tyramine and octopamine.
Bla g 4 is a male cockroach specific protein and is one of the major allergens produced by Blattella germanica (German cockroach). This protein belongs to the lipocalin family that comprises a set of proteins that characteristically bind small hydrophobic molecules and play a role in a number of processes such as: retinoid and pheromone transport, prostaglandin synthesis and mammalian immune response. Using NMR and isothermal titration calorimetry we demonstrated that Bla g 4 binds tyramine and octopamine in solution. In addition, crystal structure analysis of the complex revealed details of tyramine binding. As tyramine and octopamine play important roles in invertebrates, and are counterparts to vertebrate adrenergic transmitters, we speculate that these molecules are physiological ligands for Bla g 4. The nature of binding these ligands to Bla g 4 sheds light on the possible biological function of the protein. In addition, we performed a large-scale analysis of Bla g 4 and Per a 4 (an allergen from American cockroach) homologs to get insights into the function of these proteins. This analysis together with a structural comparison of Blag 4 and Per a 4 suggests that these proteins may play different roles and most likely bind different ligands. Accession numbers: The atomic coordinates and the structure factors have been deposited to the Protein Data Band under accession codes: 4N7C for native Bla g 4 and 4N7D for the Se-Met Bla g 4 structure.
Department of Chemistry and Biochemistry, University of South Carolina, Columbia, SC 29208, USA.
Lesa R
LR
Offermann
Department of Biological Sciences, National University of Singapore, Singapore.
Siew Leong
SL
Chan
Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA 22908, USA.
Tomasz
T
Osinski
Department of Biological Sciences, National University of Singapore, Singapore.
Yih Wan
YW
Tan
Department of Biological Sciences, National University of Singapore, Singapore.
Fook Tim
FT
Chew
Department of Biological Sciences, National University of Singapore, Singapore.
J
J
Sivaraman
Department of Biological Sciences, National University of Singapore, Singapore. Electronic address: dbsmokh@nus.edu.sg.
Yu-Keung
YK
Mok
Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA 22908, USA. Electronic address: wladek@iwonka.med.virginia.edu.
Wladek
W
Minor
Department of Chemistry and Biochemistry, University of South Carolina, Columbia, SC 29208, USA. Electronic address: chruszcz@mailbox.sc.edu.
Maksymilian
M
Chruszcz
D000485:Allergens / Q000737:chemistry / Q000276:immunology* / Q000648:ultrastructure; D000595:Amino Acid Sequence; D000818:Animals; D003058:Cockroaches / Q000276:immunology*; D003460:Crystallization; D018360:Crystallography, X-Ray; D006967:Hypersensitivity / Q000276:immunology; D019476:Insect Proteins / Q000737:chemistry / Q000276:immunology* / Q000648:ultrastructure; D008297:Male; D008958:Models, Molecular; D009655:Octopamine / Q000276:immunology*; D011485:Protein Binding; D020539:Sequence Analysis, Protein; D014439:Tyramine / Q000276:immunology*
D000485:Allergens; C097505:Blatella germanica allergen 4; D019476:Insect Proteins; D009655:Octopamine; D014439:Tyramine
Allergy; Bla g 4; Cockroach allergen; Octopamine; Per a 4; Tyramine