Delivery of BMP-2 by two clinically available apatite materials: in vitro and in vivo comparison.
Bone morphogenetic proteins (BMPs) are deposited in bone and responsible for osteoinduction. The interplay between delivery system and BMP, resulting in a characteristic release profile, is crucial for clinical success. We here report on two apatite based commercially available granules which could potentially be used in a combination product with recombinant human BMP-2 (rhBMP-2). Regardless of their similar chemistry, their interaction with rhBMP-2 differs. Deproteinized bovine bone matrix (DBBM), a clinically well-established bone substitute, has a high affinity to rhBMP-2 and releases only 50% of the growth factor during the first 2 weeks in vitro. Activity of the physio-adsorbed rhBMP-2 is indicated by an enhanced bone augmentation in vivo. In contrast, all rhBMP-2 delivered in combination with synthetic hydroxyapatite/β-tricalcium phosphate (HA/TCP) granules is released during the first 24 h. For both HA/TCP and DBBM, the released rhBMP-2 is active in vitro. Our results suggest that the different release behavior from these two apatite granules is due to the 1000-fold higher specific surface area of DBBM compared to HA/TCP.
Department of Cranio-Maxillofacial and Oral Surgery, Oral Biotechnology & Bioengineering, University Hospital Zurich, Zurich, Switzerland; Oral Biotechnology & Bioengineering, Center for Dental Medicine, University of Zürich, Zurich, Switzerland.
Patrick
P
Hänseler
Martin
M
Ehrbar
Astrid
A
Kruse
Eliane
E
Fischer
Roger
R
Schibli
Chafik
C
Ghayor
Franz E
FE
Weber
D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D018786:Bone Substitutes / Q000737:chemistry / Q000494:pharmacology; D002130:Calcium Phosphates / Q000737:chemistry / Q000494:pharmacology; D002417:Cattle; D003692:Delayed-Action Preparations / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D017886:Durapatite / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D011817:Rabbits
C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D018786:Bone Substitutes; D002130:Calcium Phosphates; D003692:Delayed-Action Preparations; C485817:beta-tricalcium phosphate; D017886:Durapatite
BMP; bone morphogenetic protein; bone regeneration; bone substitute; delivery; release system