Transcriptomic profiles of Aspergillus flavus CA42, a strain that produces small sclerotia, by decanal treatment and after recovery.
Aspergillus flavus is a ubiquitous saprophyte and is capable of producing many secondary metabolites including the carcinogenic aflatoxins. The A. flavus population that produces small sclerotia (S strain) has been implicated as the culprit for persistent aflatoxin contamination in field crops. We investigated how the plant volatile decanal, a C10 fatty aldehyde, affected the growth and development of the S strain A. flavus. Decanal treatment yielded fluffy variants lacking sclerotia and conidia and exhibiting a dosage-dependent radial colony growth. We used RNA-Seq analysis to examine transcriptomic changes caused by decanal and after removal of decanal. Mature sclerotia contained only 80% of the total transcripts detected in all samples in comparison to 94% for the decanal treated culture. Gene ontology (GO) analysis showed that decanal treatment increased expression of genes involved in oxidoreductase activity, cellular carbohydrate metabolism, alcohol metabolism and aflatoxin biosynthesis. The treatment affected cellular components associated with cell wall, and gene expression of glucanases, α-amylases, pectinesterase and peptidase required for its biosynthesis was increased. After decanal was removed, the culture resumed sclerotial production. Moreover, its GO terms significantly overlapped with those of the untreated culture; five of the enriched molecular functions, oxidoreductase activity, monooxygenase activity, electron carrier activity, heme binding, and iron binding were found in the untreated culture. The GO term of cellular component enriched was mainly integral protein constituents of the membrane. The results suggested that decanal halted development at the vegetative state rendering the fungus unable to produce conidia and sclerotia. The induced fluffy phenotype could be related to lower transcript abundance of flbB, flbD, and flbE but not to veA expression. Increased abundance of the laeA transcript in the treated culture correlated with early transcriptional activation of aflatoxin and kojic acid biosynthesis gene clusters. Expression profiles revealed subtle differences in timing of activation of the respective 55 secondary metabolite gene clusters.
Southern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, 1100 Robert E. Lee Boulevard, New Orleans, LA 70124, United States. Electronic address: perngkuang.chang@ars.usda.gov.
Perng-Kuang
PK
Chang
Southern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, 1100 Robert E. Lee Boulevard, New Orleans, LA 70124, United States.
Leslie L
LL
Scharfenstein
Southern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, 1100 Robert E. Lee Boulevard, New Orleans, LA 70124, United States.
Brian
B
Mack
Southern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, 1100 Robert E. Lee Boulevard, New Orleans, LA 70124, United States.
Jiujiang
J
Yu
Southern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, 1100 Robert E. Lee Boulevard, New Orleans, LA 70124, United States.
Kenneth C
KC
Ehrlich
D000348:Aflatoxins / Q000378:metabolism; D000447:Aldehydes / Q000494:pharmacology*; D001231:Aspergillus flavus / Q000187:drug effects* / Q000302:isolation & purification / Q000502:physiology; D002473:Cell Wall / Q000187:drug effects / Q000378:metabolism; D020869:Gene Expression Profiling; D063990:Gene Ontology; D025282:Mycelium / Q000187:drug effects / Q000502:physiology; D011753:Pyrones / Q000378:metabolism; D017423:Sequence Analysis, RNA; D013172:Spores, Fungal / Q000187:drug effects / Q000502:physiology; D015533:Transcriptional Activation
D000348:Aflatoxins; D000447:Aldehydes; D011753:Pyrones; C021170:decanaldehyde; C011890:kojic acid
Aspergillus flavus; Conidiation; Decanal; Gene ontology; Sclerotia; Transcriptome