Antimicrobial and antioxidant activities of Mimusops elengi seed extract mediated isotropic silver nanoparticles.
The present study reports the use of Mimusops elengi (M. elengi) fruit extract for the synthesis of silver nanoparticles (Ag NPs). The synthesized Ag NPs was initially noticed through visual color change from yellow to reddish brown and further confirmed by surface plasmonic resonance (SPR) band at 429 nm using UV-Visible spectroscopy. Morphology and size of Ag NPs was determined by Transmission Electron Microscopy (TEM) analysis. X-ray Diffraction (XRD) study revealed crystalline nature of Ag NPs. The prolonged stability of Ag NPs was due to capping of oxidized polyphenols which was established by Fourier Transform Infrared Spectroscopy (FTIR) study. The polyphenols present in M. elengi fruit extract was analyzed by High Pressure Liquid Chromatography (HPLC) and the results revealed the presence of ascorbic acid, gallic acid, pyrogallol and resorcinol. In order to study the role of these polyphenols in reducing Ag+ ions to Ag NPs, analyses of extracts before reduction and after reduction were carried out. In addition, the synthesized Ag NPs were tested for antibacterial and antioxidant activities against Staphylococcus aureus (S. Aureus) and Escherichia coli (E. coli). Ag NPs showed good antimicrobial activity against both gram positive (S. aureus) and gram negative (E. coli) bacteria. It also showed good antioxidant activity as compared to ascorbic acid as standard antioxidant.
Trace Elements Speciation Research Laboratory, Environmental and Analytical Chemistry Division, School of Advanced Sciences, VIT University, Vellore 632014, India.
Hoskote Anand
HA
Kiran Kumar
Trace Elements Speciation Research Laboratory, Environmental and Analytical Chemistry Division, School of Advanced Sciences, VIT University, Vellore 632014, India. Electronic address: badalmandal@vit.ac.in.
Badal Kumar
BK
Mandal
Trace Elements Speciation Research Laboratory, Environmental and Analytical Chemistry Division, School of Advanced Sciences, VIT University, Vellore 632014, India.
Kesarla
K
Mohan Kumar
Trace Elements Speciation Research Laboratory, Environmental and Analytical Chemistry Division, School of Advanced Sciences, VIT University, Vellore 632014, India.
Sireesh babu
Sb
Maddinedi
Trace Elements Speciation Research Laboratory, Environmental and Analytical Chemistry Division, School of Advanced Sciences, VIT University, Vellore 632014, India.
Tammina
T
Sai Kumar
Medical Biotechnology Division, School of Bio Sciences and Technology, VIT University, Vellore 632014, India.
Pavithra
P
Madhiyazhagan
Medical Biotechnology Division, School of Bio Sciences and Technology, VIT University, Vellore 632014, India.
Asit Ranjan
AR
Ghosh
D000890:Anti-Infective Agents / Q000737:chemistry*; D000975:Antioxidants / Q000737:chemistry*; D001205:Ascorbic Acid / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004926:Escherichia coli / Q000187:drug effects; D005707:Gallic Acid / Q000737:chemistry; D007477:Ions / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry; D008826:Microbial Sensitivity Tests; D046529:Microscopy, Electron, Transmission; D032130:Mimusops / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D011748:Pyrogallol / Q000737:chemistry; D012118:Resorcinols / Q000737:chemistry; D012639:Seeds / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013211:Staphylococcus aureus / Q000187:drug effects; D014961:X-Ray Diffraction
D000890:Anti-Infective Agents; D000975:Antioxidants; D007477:Ions; D010936:Plant Extracts; D012118:Resorcinols; D011748:Pyrogallol; D012834:Silver; D005707:Gallic Acid; D001205:Ascorbic Acid; C031389:resorcinol
Antioxidant; Isotropic; Mimusops elengi; Polyphenols; Secondary metabolites