Programming biological operating systems: genome design, assembly and activation.
The DNA technologies developed over the past 20 years for reading and writing the genetic code converged when the first synthetic cell was created 4 years ago. An outcome of this work has been an extraordinary set of tools for synthesizing, assembling, engineering and transplanting whole bacterial genomes. Technical progress, options and applications for bacterial genome design, assembly and activation are discussed.
1] J. Craig Venter Institute, La Jolla, California, USA. [2] Synthetic Genomics, Inc., La Jolla, California, USA.
Daniel G
DG
Gibson
D001709:Biotechnology / Q000379:methods; D002474:Cell-Free System; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism; D003851:Deoxyribonucleases / Q000378:metabolism; D005786:Gene Expression Regulation; D005818:Genetic Engineering / Q000379:methods*; D005822:Genetic Vectors; D016680:Genome, Bacterial; D009174:Mycoplasma / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics; D058615:Synthetic Biology / Q000379:methods*; D049490:Systems Biology
D004247:DNA; D003851:Deoxyribonucleases