Mapping three new interspecific hybrid sterile loci between Oryza sativa and O. glaberrima.
Hybrid sterility hinders the transfer of useful traits between Oryza sativa and O. glaberrima. In order to further understand the nature of interspecific hybrid sterility between these two species, a strategy of multi-donors was used to elucidate the range of interspecific hybrid sterility in this study. Fifty-nine accessions of O. glaberrima were used as female parents for hybridization with japonica cultivar Dianjingyou 1, after several backcrossings using Dianjingyou 1 as the recurrent parent and 135 BC6F1 sterile plants were selected for genotyping and deducing hybrid sterility QTLs. BC6F1 plants containing heterozygous target markers were selected and used to raise BC7F1 mapping populations for QTL confirmation and as a result, one locus for gamete elimination on chromosome 1 and two loci for pollen sterility on chromosome 4 and 12, which were distinguished from previous reports, were confirmed and designated as S37(t), S38(t) and S39(t), respectively. These results will be valuable for understanding the range of interspecific hybrid sterility, cloning these genes and improving rice breeding through gene introgression.
Food Crops Research Institute, Yunnan Academy of Agricultural Sciences (YAAS) , Kunming 650200 , P. R. China.
Peng
P
Xu
Food Crops Research Institute, Yunnan Academy of Agricultural Sciences (YAAS) , Kunming 650200 , P. R. China.
Jiawu
J
Zhou
Food Crops Research Institute, Yunnan Academy of Agricultural Sciences (YAAS) , Kunming 650200 , P. R. China.
Jing
J
Li
Food Crops Research Institute, Yunnan Academy of Agricultural Sciences (YAAS) , Kunming 650200 , P. R. China.
Fengyi
F
Hu
Food Crops Research Institute, Yunnan Academy of Agricultural Sciences (YAAS) , Kunming 650200 , P. R. China.
Xianneng
X
Deng
Yunnan Agricultural University , Kunming 650201 , P. R. China.
Sufeng
S
Feng
Yunnan Agricultural University , Kunming 650201 , P. R. China.
Guangyun
G
Ren
Yunnan Agricultural University , Kunming 650201 , P. R. China.
Zhi
Z
Zhang
Food Crops Research Institute, Yunnan Academy of Agricultural Sciences (YAAS) , Kunming 650200 , P. R. China.
Wei
W
Deng
Food Crops Research Institute, Yunnan Academy of Agricultural Sciences (YAAS) , Kunming 650200 , P. R. China.
Dayun
D
Tao
Oryza glaberrima; Oryza sativa; interspecific hybrid; sterility