[Purification of polyphenols from Sabina vulgaris antoine and its antioxidant properties].
To purify polyphenols from Sabina vulgaris and to investigate its antioxidant properties.



Polyphenols were purified from Sabina vulgaris Antoine with macroporous resin HPD-700, and the quantity of polyphenols was determined by Folin-Ciocalteu colorimetry. The antioxidant properties of polyphenols were evaluated by total antioxidant capacity (T-AOC) and its activities of scavenging DPPH (1,1 diphenyl-2-picry-hydrazyl) radicals, superoxide anion (O2·-), hydroxyl free radicals (OH·) and ferric reducing antioxidant power (FRAP).



After purification, the purity of polyphenols increased from 0.053% to 0.995%.The antioxidant properties study showed that its inhibition rate of scavenging DPPH radicals and FRAP was 151.83 U/ml and 204.59 U/ml. Its scavenging capacity for superoxide anion (O2·-) and hydroxyl free radicals (OH·) was 151.83 U/ml and 204.59 U/ml. The total antioxidant capacity was 72.68 U/ml.



Polyphenols from Sabina vulgaris Antoine have high antioxidant properties, suggesting that it worth further study of its pharmacological effects.
1. School of Medicine, Zhejiang University City College, Hangzhou 310015,China; 2. College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310018, China;
Long
L
Li
School of Medicine, Zhejiang University City College, Hangzhou 310015,China.
Gui-da
GD
Xuan
College of Science, Zhejiang University, Hangzhou 310018, China.
Ping
P
Chen
D000975:Antioxidants / Q000494:pharmacology*; D029779:Cupressaceae / Q000737:chemistry*; D059808:Polyphenols / Q000302:isolation & purification*
D000975:Antioxidants; D059808:Polyphenols