Study of the cross-contamination and survival of Salmonella in fresh apples.
The present work aimed at studying the cross contamination of apples by Salmonella during the processing of commercial fresh apples and its survival capacity on apple at room temperature. For the first study, the typical process of fresh apples was simulated at laboratory scale in which an apple that was artificially contaminated by Salmonella at different concentration levels (8, 6 and 5 log cfu/apple) was introduced in one batch and processed including a simulated transport/washing step and drying step using sponges to simulate the porous material used in the industry. Results indicated that at 8 log cfu/apple, 50% fresh apples were contaminated after processing, with all analysed environmental samples being positive for the pathogen, consisting of washing water and sponges. However, at lower inoculum levels (5-6 log cfu/apple) no cross contamination was detected in apples, and only environmental samples showed contamination by Salmonella after processing including both water and sponges. Experiments on the survival of Salmonella on apple showed that the pathogen was capable to survive for 12 days, only showing a significant drop at the end of the experiment. Finally, two-class attribute sampling plans were assessed as tool to detect Salmonella in different contamination scenarios in fresh apple. This analysis indicated that with the highest inoculum level, a total of 16 apples would be needed to reach 95% of detecting Salmonella (i.e. lot rejection). In turn, when low levels were assessed (5-6 log cfu/apple), a large number of apples (n=1021) would have to be sampled to obtain the same confidence level (95%). If the environment is sampled (i.e. water and sponges), a lower number of samples would be needed to detect the pathogen. However, the feasibility of environmental sampling has not been assessed from a practical point of view. Overall, the results in this study evidenced that cross contamination by Salmonella might occur during processing of fresh apples and subsequently, the pathogen might survive for a noticeable period of time.
Department of Food Science and Technology, University of Córdoba, Campus de Rabanales, C-1, 14014 Córdoba, Spain; International Campus of Excellence in the AgriFood Sector (CeIA3), Spain. Electronic address: b42perof@uco.es.
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F
Perez-Rodriguez
Norwegian Veterinary Institute, Section for Bacteriology-Food and GMO, P.O. Box 750 Sentrum, 0106 Oslo, Norway.
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M
Begum
Norwegian Veterinary Institute, Section for Bacteriology-Food and GMO, P.O. Box 750 Sentrum, 0106 Oslo, Norway.
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GS
Johannessen
D005511:Food Handling; D005638:Fruit / Q000382:microbiology*; D027845:Malus / Q000382:microbiology*; D012475:Salmonella / Q000302:isolation & purification / Q000502:physiology*
Cross contamination; Fruit; Predictive microbiology; Salmonella; Sampling plan; Weibull model