Survey of ochratoxin A in rice from Iran using affinity column cleanup and HPLC with fluorescence detection.
This paper records the occurrence and levels of ochratoxin A (OTA) in rice using a HPLC technique preceded by an immunoaffinity clean-up step. The method was based on the extraction of finely ground rice sample with an acetonitrile/water (60 : 40, v/v) solution. Recovery was 98.9% while the limit of detection (LOD) was 0.2 ng g⁻¹. A total of 182 rice samples were analyzed with a frequency of contamination of 6%. Levels of OTA in positive samples ranged 0.2-4.8 ng g⁻¹, with an average contamination of all analyzed samples of 1.6 ng g⁻¹.
a Testa Quality Control Laboratory , North-East Food Industrial Technology and Biotechnology Park , Mashhad , Iran.
J
J
Feizy
H R
HR
Beheshti
S S
SS
Fakoor Janati
N
N
Khoshbakht Fahim
D053000:Analytic Sample Preparation Methods; D002138:Calibration; D002273:Carcinogens / Q000032:analysis*; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D005506:Food Contamination; D005513:Food Inspection; D019983:Guideline Adherence; D006291:Health Policy; D006293:Health Promotion; D006801:Humans; D007492:Iran; D057230:Limit of Detection; D009793:Ochratoxins / Q000032:analysis*; D012275:Oryza / Q000737:chemistry* / Q000191:economics; D015203:Reproducibility of Results; D012639:Seeds / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
D002273:Carcinogens; D009793:Ochratoxins; C025589:ochratoxin A
mycotoxins; ochratoxin A; rice