An expanded set of fluorogenic sulfatase activity probes.
Fluorogenic probes that are activated by an enzymatic transformation are ideally suited for profiling enzyme activities in biological systems. Here, we describe two fluorogenic enzyme probes, 3-O-methylfluorescein-sulfate and resorufin-sulfate, that can be used to detect sulfatases in mycobacterial lysates. Both probes were validated with a set of commercial sulfatases and used to reveal species-specific sulfatase banding patterns in a gel-resolved assay of mycobacterial lysates. The fluorogenic probes described here are suitable for various assays and provide a starting point for creating new sulfatase probes with improved selectivity for mycobacterial sulfatases.
Departments of Chemistry and Molecular and Cell Biology, Howard Hughes Medical Institute, University of California, B84 Hildebrand Hall, #1460, Berkeley, CA 94720 (USA).
Elizabeth L
EL
Smith
Carolyn R
CR
Bertozzi
Kimberly E
KE
Beatty
D000818:Animals; D021902:Enterobacter aerogenes / Q000201:enzymology; D004789:Enzyme Activation; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism*; D006372:Helix, Snails / Q000201:enzymology; D015394:Molecular Structure; D012758:Shellfish; D013429:Sulfatases / Q000032:analysis* / Q000378:metabolism*
D005456:Fluorescent Dyes; D013429:Sulfatases
Mycobacterium tuberculosis; fluorescent probes; hydrolases; protein imaging; sulfatases