Effectiveness of depuration for hepatitis A virus removal from mussels (Mytilus galloprovincialis).
The efficacy and kinetic of depuration of hepatitis A virus (HAV) were evaluated under experimental conditions with Mediterranean mussels (Mytilus galloprovincialis) subjected previously to bioaccumulation processes. Seven independent trials (70kg of mussels each) were performed in a closed experimental system using two different water temperatures (13 and 17°C) during 7days. The real time RT-PCR technique with TaqMan probes was used for viral quantification. Qualitative infectivity assays were conducted to test the presence of infectious viral particles at the end of the depuration period. The depuration trials showed an average reduction of HAV levels of aproximately 1.1 Log units (>90%). However, the average final viral loads in shellfish samples remain at relatively high levels (6.5×10(3) RNA copies/g digestive tissue) and still infectious. A positive correlation between the initial and the final numbers of the viral RNA copies was observed. The reduction of HAV showed a two-phase removal kinetic, an initial logarithmic trendline, with a rapid reduction of viruses during the first 24-48h of depuration, and a subsequent stabilization with a slower depuration rate until the end of the process.
Departamento de Microbiología y Parasitología, CIBUS-Facultad de Biología, Universidad de Santiago de Compostela, 15782, Santiago de Compostela, Spain.
David
D
Polo
Instituto Tecnolóxico para o Control do Medio Mariño de Galicia (INTECMAR), 36611, Vilagarcía de Arousa, Spain.
Cristina
C
Álvarez
Consello Regulador da Denominación de Orixe Protexida do Mexillón de Galicia, 36600 Vilagarcía de Arousa, Spain.
Ángeles
Á
Longa
Departamento de Microbiología y Parasitología, CIBUS-Facultad de Biología, Universidad de Santiago de Compostela, 15782, Santiago de Compostela, Spain. Electronic address: jesus.romalde@usc.es.
Jesús L
JL
Romalde
D000704:Analysis of Variance; D000818:Animals; D002460:Cell Line; D003666:Decontamination / Q000379:methods* / Q000592:standards; D005516:Food Microbiology / Q000379:methods* / Q000592:standards; D030041:Hepatitis A virus / Q000502:physiology*; D049878:Mytilus / Q000821:virology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013696:Temperature; D013995:Time; D019562:Viral Load
Depuration; Hepatitis A virus; Infectivity; Mussels; Real time RT-PCR