Preparation of surface-imprinted polymer grafted with water-compatible external layer via RAFT precipitation polymerization for highly selective and sensitive electrochemical determination of brucine.
A novel brucine imprinted polymer was prepared on multi-walled carbon nanotubes by reversible addition-fragmentation chain transfer (RAFT) precipitation polymerization. The polymer was further grafted with hydrophilic poly(glycerol monomethacrylate) brushes to improve its water-compatibility. The obtained molecularly imprinted material showed enhanced accessibility to brucine and improved selective recognition property in water medium. When the material was supported on an ionic liquid functionalized graphene coated glassy carbon electrode for the electrochemical determination of brucine, the resulting electrochemical sensor presented good analytical performance. Under the optimized conditions, the peak current was linear to brucine concentration in the ranges of 0.006-0.6 μM and 0.6-5.0 μM with sensitivities of 15.3 μA/μMmm(2) and 5.4 μA/μM mm(2), respectively; the detection limit was 2 nM (S/N=3). The sensor was successfully applied to the determination of brucine in practical samples and the recovery for the standards added was 94-104%.
Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, Hubei Province, PR China.
Lijuan
L
Zhao
Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, Hubei Province, PR China.
Faqiong
F
Zhao
Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, Hubei Province, PR China. Electronic address: bzzeng@whu.edu.cn.
Baizhao
B
Zeng
D003217:Conductometry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005590:Fractional Precipitation / Q000379:methods*; D057927:Hydrophobic and Hydrophilic Interactions; D008768:Methylmethacrylates / Q000737:chemistry*; D054802:Molecular Imprinting / Q000379:methods*; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012996:Solutions; D013331:Strychnine / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry; D013499:Surface Properties; D014867:Water / Q000737:chemistry*
D008768:Methylmethacrylates; D037742:Nanotubes, Carbon; D012996:Solutions; C043583:glyceryl methyl methacrylate; D014867:Water; C083806:brucine; D013331:Strychnine
Brucine; Electrochemical sensor; Molecularly imprinted polymer; Poly(glycerol monomethacrylate); RAFT polymerization