Characterization of Hybrid Bioactive Glass-polyvinyl Alcohol Scaffolds Containing a PTHrP-derived Pentapeptide as Implants for Tissue Engineering Applications.
Hybrid foam (BG-PVA) with 50 % Bioactive glass (BG) and 50 % polyvinyl alcohol (PVA) was prepared by sol-gel process to produce scaffolds for bone tissue engineering. The pore structure of hydrated foams was evaluated by 3-D confocal microscopy, confirming 70% porosity and interconnected macroporous network. In this study, we assessed the putative advantage of coating with osteostatin pentapeptide into BG-PVA hybrid scaffolds to improve their bioactivity. In vitro cell culture experiments were performed using mouse pre-osteoblastic MC3T3-E1 cell line. The exposure to osteostatin loaded-BG-PVA scaffolds increase cell proliferation in contrast with the unloaded scaffolds. An in vivo study was selected to implant BG-PVA scaffolds, non-coated (Group A) or coated (Group B) with osteostatin into non critical bone defect at rabbit femur. Both groups showed new compact bone formation on implant surface, with lamellae disposed around a haversian canal forming osteons-like structure. We observed signs of inflammation around the implanted unloaded scaffold at one month, but resolved at 3 months. This early inflammation did not occur in Group B; supporting the notion that osteostatin may act as anti-inflammatory inhibitor. On the other hand, Group B showed increased bone formation, as depicted by many new trabeculae partly mineralized in the implant regenerating area, incipient at 1 month and more evident at 3 months after implantation. PVA/BG hybrid scaffolds present a porous structure suitable to support osteoblast proliferation and differentiation. Our in vitro and in vivo findings indicate that osteostatin coating improves the osteogenic features of these scaffolds.
Laboratorio de Biología Osteoarticular, Ingeniería Tisular y Terapias Emergentes (LABOATEM), Facultad de Ciencias Médicas de la Universidad Nacional de Rosario, Rosario, Argentina.
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Coletta
Laboratorio de Metabolismo Mineral y Óseo, Instituto de Investigación Sanitaria (IIS)-Fundación Jiménez Díaz, and Red Temática de Investigación Cooperativa de Envejecimiento y Fragilidad (RETICEF), Instituto de Salud Carlos III, Madrid, Spain.
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Lozano
Laboratorio de Biomateriais, Departamento de Engenharia Metalúrgica e de Materiais, Universidade Federal de Minas Gerais, Belo Horizonte, Brasil.
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Rocha-Oliveira
Laboratorio de Biología Osteoarticular, Ingeniería Tisular y Terapias Emergentes (LABOATEM), Facultad de Ciencias Médicas de la Universidad Nacional de Rosario, Rosario, Argentina.
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Mortarino
Laboratorio de Biología Osteoarticular, Ingeniería Tisular y Terapias Emergentes (LABOATEM), Facultad de Ciencias Médicas de la Universidad Nacional de Rosario, Rosario, Argentina.
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Bumaguin
Laboratorio de Biología Osteoarticular, Ingeniería Tisular y Terapias Emergentes (LABOATEM), Facultad de Ciencias Médicas de la Universidad Nacional de Rosario, Rosario, Argentina.
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Vitelli
Instituto Biología Rosario (IBR), Universidad Nacional de Rosario, Argentina.
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Vena
Laboratorio de Patología Experimental e Ingeniería de Tejidos. PROIMI-CONICET, Tucumán, Argentina ; Laboratorio de Patología Experimental, Diagnóstico e Ingeniería de Tejidos, Facultad de Odontología, Universidad de Tucumán, Tucumán, Argentina.
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Missana
Laboratorio de Patología Experimental e Ingeniería de Tejidos. PROIMI-CONICET, Tucumán, Argentina ; Laboratorio de Patología Experimental, Diagnóstico e Ingeniería de Tejidos, Facultad de Odontología, Universidad de Tucumán, Tucumán, Argentina.
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Jammal
Laboratorio de Metabolismo Mineral y Óseo, Instituto de Investigación Sanitaria (IIS)-Fundación Jiménez Díaz, and Red Temática de Investigación Cooperativa de Envejecimiento y Fragilidad (RETICEF), Instituto de Salud Carlos III, Madrid, Spain.
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Portal-Núñez
Laboratorio de Biomateriais, Departamento de Engenharia Metalúrgica e de Materiais, Universidade Federal de Minas Gerais, Belo Horizonte, Brasil.
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Pereira
Laboratorio de Metabolismo Mineral y Óseo, Instituto de Investigación Sanitaria (IIS)-Fundación Jiménez Díaz, and Red Temática de Investigación Cooperativa de Envejecimiento y Fragilidad (RETICEF), Instituto de Salud Carlos III, Madrid, Spain.
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Esbrit
Laboratorio de Biología Osteoarticular, Ingeniería Tisular y Terapias Emergentes (LABOATEM), Facultad de Ciencias Médicas de la Universidad Nacional de Rosario, Rosario, Argentina.
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Feldman
Bioactive glass; bone regeneration; femur bone lesion; hybrid matrix; polyvinyl alcohol.