Preparation of Tween 80-Zn/Al-levodopa-layered double hydroxides nanocomposite for drug delivery system.
We incorporated anti-Parkinsonian drug, levodopa (dopa), in Zn/Al-LDH by coprecipitation method to form dopa-LDH nanocomposite. Further coating of Tween-80 on the external surfaces of dopa-LDH nanocomposite was achieved through the oxygen of C=O group of Tween-80 with the layer of dopa-LDH nanocomposite. The final product is called Tween-dopa-LDH nanocomposite. The X-ray diffraction indicates that the Tween-dopa-LDH nanocomposite was formed by aggregation structure. From the TGA data, the Tween-80 loading on the surface of LDH and dopa-LDH was 8.6 and 7.4%, respectively. The effect of coating process on the dopa release from Tween-dopa-LDH nanocomposite was also studied. The release from Tween-dopa-LDH nanocomposite shows slower release compared to the release of the drug from dopa-LDH nanocomposite as done previously in our study, presumably due to the retarding shielding effect. The cell viability study using PC12 showed improved viability with Tween-80 coating on dopa-LDH nanocomposite as studied by mitochondrial dehydrogenase activity (MTT assay).
Laboratory of Vaccine and Immunotherapeutic, Institute of Bioscience, Universiti Putra Malaysia, 43400 Selangor, Malaysia.
Aminu Umar
AU
Kura
Laboratory of Molecular Biomedicine, Institute of Bioscience, Universiti Putra Malaysia, 43400 Selangor, Malaysia ; Faculty of pharmacy, Isra university, P.O. Box 22, Amman 11622, Jordan.
Samer Hasan
SH
Hussein-Al-Ali
Materials Synthesis and Characterization Laboratory, Institute of Advanced Technology (ITMA), Universiti Putra Malaysia, 43400 Selangor, Malaysia.
Mohd Zobir
MZ
Hussein
Laboratory of Vaccine and Immunotherapeutic, Institute of Bioscience, Universiti Putra Malaysia, 43400 Selangor, Malaysia ; Faculty of Medicine and Health Science, Pharmacology Unit, Universiti Putra Malaysia, 43400 Selangor, Malaysia.
Sharida
S
Fakurazi
D000535:Aluminum / Q000737:chemistry*; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D004337:Drug Carriers; D016503:Drug Delivery Systems; D006878:Hydroxides / Q000737:chemistry*; D007700:Kinetics; D007980:Levodopa / Q000008:administration & dosage*; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D016716:PC12 Cells; D011136:Polysorbates / Q000737:chemistry*; D051381:Rats; D017550:Spectroscopy, Fourier Transform Infrared; D013816:Thermodynamics; D014961:X-Ray Diffraction; D015032:Zinc / Q000737:chemistry*
D004337:Drug Carriers; D006878:Hydroxides; D011136:Polysorbates; D007980:Levodopa; D000535:Aluminum; D015032:Zinc