[Comparative analysis of early reconstitution of T-lymphocyte subsets and T-cell receptor excision cycles in patients after myeloablative unrelated cord blood and sibling donor transplantation].
This study was purposed to comparatively analyze the early T-lymphocyte subsets and T-cell receptor excision cycles (TREC) reconstruction in recipients with hematologic malignancies after myeloablative unrelated cord blood transplantation (UCBT) and sibling donor bone marrow and/or peripheral blood stem cell transplantation (BMT/PBSCT). The peripheral blood T lymphocyte subsets were detected using flow cytometry and TREC were detected using real-time quantitative PCR for 40 patients with hematologic malignancies in the first six months after myeloablative allogenic hematopoietic stem cell transplantation. The results showed that in the first month after transplantation, the absolute counts of CD3(+), CD3(+) CD4(+), CD3(+) CD8(+) cells were lower significantly in the UCBT group than those in the BMT/PBSCT group. And later the absolute counts of CD3(+), CD3(+)CD4(+), CD3(+)CD8(+) cells were not different between two groups. The ratio of CD3(+)T subset in the peripheral blood lymphocytes of the UCBT recipients was lower, but the difference was not statistically significant within 2 months after transplantation. The ratio of CD3(+)CD4(+) cells in the patients received the UCBT and BMT/PBSCT decreased obviously since engraftment happened. The CD3(+)CD4(+) cells on the 2 months after transplantation fell to the lowest level, then gradually increased, but did not reach to the normal level until 6 months after transplantation. CD3(+)CD8(+)cells were well reconstituted, rising to normal at the engraftment after transplantation, with a low CD4(+): [KG-*2] CD8(+) ratio over the first 6 months after transplantation. Compared with the BMT/ PBSCT group, the naive T cells (CD3(+)CD4(+)CD45RA(+)CD62L(+)) were more in the first month after transplantation and the terminally differentiated effector memory T cells (CD3(+)CD4(+)CD45RA(+)CD62L(-)) were more at the 3 month after transplantation in the UCBT group, and those were significantly more than the normal control group. TREC were lower and did not recovered until 6 months after transplantation in the recipients of the two groups. It is concluded that compared with sibling donor's BMT/PBSCT, early T cell reconstitution significantly delayed after UCBT, but the terminally differentiated effector memory T cells are higher after transplantation, and thus play a anti-infective or anti-leukemia role.
Department of Hematology, Anhui Provincial Hospital Affiliated to Anhui Medical University, Hefei 230001, Anhui Province, China.
Hao
H
Nie
Department of Hematology, Anhui Provincial Hospital Affiliated to Anhui Medical University, Hefei 230001, Anhui Province, China.
Jian
J
Wang
Department of Hematology, Anhui Provincial Hospital Affiliated to Anhui Medical University, Hefei 230001, Anhui Province, China.
You-Jia
YJ
Lu
Department of Hematology, Anhui Provincial Hospital Affiliated to Anhui Medical University, Hefei 230001, Anhui Province, China.
Chang-Cheng
CC
Zheng
Department of Hematology, Anhui Provincial Hospital Affiliated to Anhui Medical University, Hefei 230001, Anhui Province, China.
Bao-Lin
BL
Tang
Department of Hematology, Anhui Provincial Hospital Affiliated to Anhui Medical University, Hefei 230001, Anhui Province, China.
Wen
W
Yao
Department of Hematology, Anhui Provincial Hospital Affiliated to Anhui Medical University, Hefei 230001, Anhui Province, China.
Xiao-Yu
XY
Zhu
Department of Hematology, Anhui Provincial Hospital Affiliated to Anhui Medical University, Hefei 230001, Anhui Province, China.
Ping
P
Qiang
Department of Hematology, Anhui Provincial Hospital Affiliated to Anhui Medical University, Hefei 230001, Anhui Province, China.
Lei
L
Zhang
Department of Hematology, Anhui Provincial Hospital Affiliated to Anhui Medical University, Hefei 230001, Anhui Province, China. E-mail: liuhuilan@medmail.com.cn.
Hui-Lan
HL
Liu
Department of Hematology, Anhui Provincial Hospital Affiliated to Anhui Medical University, Hefei 230001, Anhui Province, China.
Zi-Min
ZM
Sun
D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D005260:Female; D005312:Fetal Blood / Q000637:transplantation*; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D006801:Humans; D008297:Male; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D055815:Young Adult
D011948:Receptors, Antigen, T-Cell