Fluorescence immunoassay of octachlorostyrene based on Förster resonance energy transfer between CdTe quantum dots and rhodamine B.
Octachlorostyrene (OCS), a persistent and bioaccumulative toxicant (PBT), was assayed by fluorescence immunoassay based on the Förster resonance energy transfer (FRET) between CdTe quantum dots (QDs) and rhodamine B-labeled OCS (RB-OCS). Anti-OCS antibody produced in this lab is adsorbed on a microtiter plate. The RB-OCS competes with OCS for the highly specific immunoreaction with the anti-OCS antibodies adsorbed on the microtiter plate. The solution is then isolated and mixed with CdTe QDs as fluorescent donor which excite the emission of RB-OCS through FRET. As a result, the emission of CdTe QDs at 530 nm decreases, whereas the emission of RB-OCS at 580 nm increases. The ratio of fluorescence intensity at 580 nm to that at 530 nm is proportional to the RB-OCS concentration at a fixed CdTe QDs concentration, and consequently proportional to the OCS concentration. Selective and sensitive responses to OCS are achieved with a linear range of 8-80 nM and a LOD of 3.8 nM. Because OCS is quantified based on the fluorescence ratio, the sensor-to-sensor difference is greatly eliminated, making the proposed method a useful approach for in site scanning of OCS.
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China.
Xin
X
Wang
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China.
Pengtao
P
Sheng
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China.
Liping
L
Zhou
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China.
Xi
X
Tong
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China.
Lei
L
Shi
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China. Electronic address: qycai0001@hnu.edu.cn.
Qingyun
Q
Cai
D015374:Biosensing Techniques / Q000295:instrumentation*; D019187:Cadmium Compounds / Q000737:chemistry*; D004784:Environmental Monitoring / Q000295:instrumentation / Q000379:methods; D004785:Environmental Pollutants / Q000032:analysis / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D031541:Fluorescence Resonance Energy Transfer / Q000295:instrumentation* / Q000379:methods; D045663:Quantum Dots; D012235:Rhodamines / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000295:instrumentation*; D013343:Styrenes / Q000032:analysis* / Q000737:chemistry; D013691:Tellurium / Q000737:chemistry*
D019187:Cadmium Compounds; D004785:Environmental Pollutants; D012235:Rhodamines; D013343:Styrenes; C014138:octachlorostyrene; C029773:rhodamine B; D013691:Tellurium; C028337:cadmium telluride
CdTe QDs; FRET; Immunoassay; Octachlorostyrene