Effects of gold nanoparticles on erythrocyte hemolysis.
We studied hemolytic activity of gold nanoparticles added to the whole blood (ex vivo) and of nanoparticles coated and not coated with plasma components on erythrocytes in hypotonic medium (osmotic hemolysis) in vitro. Gold nanoparticles did not stimulate erythrocyte hemolysis after 4-h incubation with the whole blood ex vivo. Hemolysis tended to increase in the presence of small gold nanoparticles (5, 10, 20 nm) at the maximum concentration of 20 μM (by gold content) used in our study in comparison with the control. This tendency was detected during the 1st hour of the nanoparticles incubation with blood. Gold nanoparticles in the used concentrations (up to 20 μM of gold) coated with plasma components after preincubation with autologous plasma and nanoparticles without coating caused no osmotic hemolysis of erythrocytes in vitro.
Laboratory of Biophysical Bases of Disease, Research Institute of Physico-Chemical Medicine, Federal Biomedical Agency of Russia, Moscow, Russia, aseychev@mail.ru.
A V
AV
Aseichev
O A
OA
Azizova
E M
EM
Beckman
O I
OI
Skotnikova
L B
LB
Dudnik
O N
ON
Shcheglovitova
V I
VI
Sergienko
D004912:Erythrocytes / Q000187:drug effects*; D006046:Gold / Q000737:chemistry / Q000633:toxicity*; D006461:Hemolysis; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry / Q000633:toxicity*; D009997:Osmotic Pressure; D010316:Particle Size
D006046:Gold