Extraction of aflatoxins from liquid foodstuff samples with polydopamine-coated superparamagnetic nanoparticles for HPLC-MS/MS analysis.
A facile magnetic solid phase extraction (MSPE) of aflatoxins (AFs) from liquid samples was developed using polydopamine-coated magnetic nanoparticles (PD-MNPs) as the adsorbent. PD-MNPs were prepared from amine-terminated MNPs and dopamine via an in situ oxidative self-polymerization approach. Under the selected MSPE conditions, extraction yields ranging from 59.3% for AF G2 to 89.0% for AF B1 were obtained with good repeatability. Coupled with HPLC-MS/MS quantification, the MSPE procedure serves not only for sample cleanup but also for AFs enrichment that is highly desired for trace analysis. The proposed MSPE-HPLC-MS/MS method had a linear calibration curve in the concentration range from 0.00600 to 3.00 ng/mL aflatoxin and limits of detection of 0.0012 ng/mL for AF B1, AF B2, and AF G1, and 0.0031 ng/mL for AF G2.
Department of Chemistry and Biochemistry, Jackson State University , 1400 Lynch Street, Jackson, Mississippi 39217, United States.
Cassandra
C
McCullum
Paul
P
Tchounwou
Li-Sheng
LS
Ding
Xun
X
Liao
Yi-Ming
YM
Liu
D000327:Adsorption; D000348:Aflatoxins / Q000032:analysis* / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005506:Food Contamination / Q000032:analysis; D007211:Indoles / Q000737:chemistry; D058185:Magnetite Nanoparticles / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D052616:Solid Phase Extraction / Q000295:instrumentation / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014920:Wine / Q000032:analysis*
D000348:Aflatoxins; D007211:Indoles; D058185:Magnetite Nanoparticles; D011108:Polymers; C568283:polydopamine