Identification and differential expression of hepatopancreas microRNAs in red swamp crayfish fed with emodin diet.
Using high-throughput Illumina Solexa system, the differential miRNA expressions from hepatopancreas in red swamp crayfish (Procambarus clarkii) fed with diets containing 0 (control) and 75 mg emodin kg(-1) (trial) were identified, respectively. As a result, 13,335,928 raw reads from the control sample and 14,938,951 raw reads from the trial sample were obtained while 13,053,344 (98.77%) and 14,517,522 (98.34%) small RNA were identified, respectively. 106 mature miRNAs (belonging to 68 miRNA gene families) were identified. 35 miRNAs displayed significantly differential expressions between two libraries. Of these, comparing to the control library, 6 miRNAs were significantly up-regulated and 29 miRNAs were significantly down-regulated. Moreover, 5 novel miRNAs (2 from control sample, 3 from trial sample) and target genes were predicted. GO analysis suggested that these miRNAs might be involved in innate immune response, growth, metabolism, cellular process, biological regulation and stimulus response. Our knowledge from this study could contribute to a better understanding of the miRNAs roles in regulating innate immune response and the study of miRNA function in crayfish.
Key Lab of Aquatic Animal Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Wei-Na
WN
Xu
Key Lab of Aquatic Animal Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: wbliu@njau.edu.cn.
Wen-Bin
WB
Liu
Key Lab of Aquatic Animal Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Wei-Wei
WW
Yang
Key Lab of Aquatic Animal Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Ding-Dong
DD
Zhang
Key Lab of Aquatic Animal Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Guang-Zhen
GZ
Jiang
D000821:Animal Feed / Q000032:analysis; D000818:Animals; D003400:Astacoidea / Q000187:drug effects* / Q000235:genetics* / Q000254:growth & development / Q000276:immunology; D004032:Diet; D019587:Dietary Supplements / Q000032:analysis; D004642:Emodin / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D043143:Hepatopancreas / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D059014:High-Throughput Nucleotide Sequencing; D007113:Immunity, Innate / Q000187:drug effects*; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011897:Random Allocation; D017423:Sequence Analysis, RNA
D035683:MicroRNAs; D004642:Emodin
Emodin; Immunoregulation; MicroRNA; Procambarus clarkii