Development and validation of benomyl birdseed agar for the isolation of Cryptococcus neoformans and Cryptococcus gattii from environmental samples.
One of the difficulties of isolating Cryptococcus neoformans and Cryptococcus gattii from environmental samples is the abundant overgrowth of other yeast and mold species that occurs on the plates. Here we report the application of benomyl to Guizotia abyssinica seed extract growth medium to improve the isolation of C. neoformans and C. gattii from environmental samples. We validated this medium by recovering C. neoformans and C. gattii from convenience soils and swabs from a region of the United States where these yeasts are endemic.
Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, GA, USA.
Cau D
CD
Pham
Stacey
S
Ahn
Lance A
LA
Turner
Ron
R
Wohrle
Shawn R
SR
Lockhart
D000362:Agar; D019659:Asteraceae / Q000737:chemistry; D001542:Benomyl / Q000302:isolation & purification / Q000378:metabolism; D056285:Cryptococcus gattii / Q000302:isolation & purification*; D003455:Cryptococcus neoformans / Q000302:isolation & purification*; D003470:Culture Media / Q000737:chemistry*; D004783:Environmental Microbiology; D010936:Plant Extracts / Q000302:isolation & purification; D012639:Seeds / Q000737:chemistry; D014481:United States
D003470:Culture Media; D010936:Plant Extracts; D000362:Agar; D001542:Benomyl
Cryptococcus gattii; Cryptococcus neoformans; benomyl; birdseed agar