Mycelial antineoplastic activity of Agaricus blazei.
Basidiocarp of Agaricus blazei (=Agaricus brasiliensis; =Agaricus subrufescens) is used as teas or capsules due to its antineoplastic effect but there are few reports of using mycelium for this purpose. The objective of this study was to evaluate the antineoplastic activity on sarcoma 180 cells implanted in mice of two forms of preparation of the mycelium from two A. blazei strains grown in culture medium with different concentrations of isolated soy protein. Mycelia were grown in Pontecorvo medium with different concentrations of isolated soybean protein (ISP). Mycelial hot water extract, moistened mycelial powder, hot water extract of green tea, Ifosfamida(®) (ifosfamide drug), and saline solution were administered daily by gavage in mice with sarcoma 180 cells to evaluate antineoplastic activity. It was concluded that antineoplastic activity was the same for both strains, except when used as moistened mycelial powder, which rules out the use of mycelial powder in capsules. Mycelial hot water extract had high antineoplastic activity with lower metabolic demand on the spleen and maintenance of normal blood parameters. Mycelial growth in different ISP concentrations had the same antineoplastic activity. Also the vegetative mycelium was as effective as the basidiocarp for sarcoma 180 tumor inhibition. Green tea was as effective as mycelial hot water extract.
Molecular Biology Laboratory, Paranaense University, Praça Mascarenhas de Moraes, Umuarama, PR, 4282, Brazil.
Míria Benetati Delgado
MB
Bertéli
Suzana Harue
SH
Umeo
André
A
Bertéli
Juliana Silveira
JS
do Valle
Giani Andrea
GA
Linde
Nelson Barros
NB
Colauto
D000364:Agaricus / Q000737:chemistry*; D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000627:therapeutic use*; D003470:Culture Media / Q000737:chemistry; D004333:Drug Administration Routes; D004339:Drug Compounding; D005260:Female; D007069:Ifosfamide / Q000627:therapeutic use; D051379:Mice; D025282:Mycelium / Q000737:chemistry*; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage* / Q000627:therapeutic use*; D012510:Sarcoma 180 / Q000188:drug therapy* / Q000473:pathology; D030262:Soybean Proteins / Q000494:pharmacology
D000970:Antineoplastic Agents; D003470:Culture Media; D010936:Plant Extracts; D030262:Soybean Proteins; D007069:Ifosfamide