Evaluation of Paecilomyces variotii potential in bioethanol production from lignocellulose through consolidated bioprocessing.
The ascomycete Paecillomyces variotii was evaluated for the first time as a candidate species for the production of bioethanol from lignocellulose through consolidated bioprocessing (CBP) approaches. The examined strain (ATHUM 8891) revealed all the necessary phenotypic characteristics required for 2nd generation biofuel production. The fungus is able to efficiently ferment glucose and xylose to ethanol, with yields close to the theoretical maximum. Nitrogen supplementation greatly affected ethanol production with nitrate-nitrogen presenting the best results. Notably, ethanol yield on xylose fermentation was higher than that of glucose, while in co-fermentation of glucose-xylose mixtures no distinguished diauxic behavior was observed. Furthermore, the fungus seems to possess the necessary enzyme factory for the degradation of lignocellulosic biomass, as it was able to grow and produce ethanol on common agro-industrial derivatives. Overall, the results of our study indicate that P. variotii is a new and possibly powerful candidate for CBP applications.
Microbial Biotechnology Unit, Sector of Botany, Department of Biology, National and Kapodistrian University of Athens, University Campus, 15784 Zografou, Attica, Greece.
Anastasia
A
Zerva
Microbial Biotechnology Unit, Sector of Botany, Department of Biology, National and Kapodistrian University of Athens, University Campus, 15784 Zografou, Attica, Greece.
Alexander L
AL
Savvides
Microbial Biotechnology Unit, Sector of Botany, Department of Biology, National and Kapodistrian University of Athens, University Campus, 15784 Zografou, Attica, Greece.
Efstathios A
EA
Katsifas
Microbial Biotechnology Unit, Sector of Botany, Department of Biology, National and Kapodistrian University of Athens, University Campus, 15784 Zografou, Attica, Greece.
Amalia D
AD
Karagouni
Microbial Biotechnology Unit, Sector of Botany, Department of Biology, National and Kapodistrian University of Athens, University Campus, 15784 Zografou, Attica, Greece. Electronic address: dhatzini@biol.uoa.gr.
Dimitris G
DG
Hatzinikolaou
D000332:Aerobiosis / Q000187:drug effects; D056804:Biofuels / Q000382:microbiology*; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D002244:Carbon / Q000494:pharmacology; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation / Q000187:drug effects; D008031:Lignin / Q000378:metabolism*; D009584:Nitrogen / Q000494:pharmacology; D010143:Paecilomyces / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*
D056804:Biofuels; C036909:lignocellulose; D000431:Ethanol; D002244:Carbon; D008031:Lignin; D009584:Nitrogen
Bioethanol; Consolidated bioprocessing; Glucose and xylose fermentation; Lignocellulose; Paecilomyces variotii