Monoglucosyl-rutin as a potential radioprotector in mammalian cells.
In the present study, the role of monoglucosyl‑rutin as a potential radioprotector was investigated using mammalian cell culture models. Cell survival and DNA damage were assessed using colony formation, sister chromatid exchange and γH2AX assays. It was demonstrated that monoglucosyl‑rutin was able to increase cell survival when exposed to ionizing radiation, possibly by decreasing the amount of base damage experienced by the cell. However, the present study also demonstrated that, despite monoglucosyl‑rutin exhibiting radioprotective effects at low doses, high doses of monoglucosyl‑rutin led to a decrease in plating efficiency and an increased doubling time. This effect may be due to double‑strand breaks caused by high concentrations of monoglucosyl‑rutin.
Department of Nuclear Engineering and Management, School of Engineering, The University of Tokyo, Tokyo 113‑8656, Japan.
Shigeaki
S
Sunada
Department of Bioengineering, School of Engineering, The University of Tokyo, Tokyo 113‑8656, Japan.
Hiroshi
H
Fujisawa
Department of Environmental and Radiological Health Sciences, Colorado State University, Fort Collins, CO 80523, USA.
Ian M
IM
Cartwright
Department of Environmental and Radiological Health Sciences, Colorado State University, Fort Collins, CO 80523, USA.
Junko
J
Maeda
Department of Environmental and Radiological Health Sciences, Colorado State University, Fort Collins, CO 80523, USA.
Colleen A
CA
Brents
Center for Disease Biology and Integrative Medicine, Faculty of Medicine, The University of Tokyo, Tokyo 113‑8656, Japan.
Kazue
K
Mizuno
Research and Development Group, Toyo Sugar Refining Co., Ltd., Yoto Bldg., Tokyo 103‑0046, Japan.
Yasushi
Y
Aizawa
Department of Environmental and Radiological Health Sciences, Colorado State University, Fort Collins, CO 80523, USA.
Takamitsu A
TA
Kato
Department of Nuclear Engineering and Management, School of Engineering, The University of Tokyo, Tokyo 113‑8656, Japan.
Mitsuru
M
Uesaka
D000818:Animals; D016466:CHO Cells; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects* / Q000528:radiation effects; D006224:Cricetinae; D003412:Cricetulus; D004249:DNA Damage / Q000187:drug effects* / Q000528:radiation effects; D005720:Gamma Rays; D006657:Histones / Q000235:genetics; D006801:Humans; D011837:Radiation-Protective Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D012431:Rutin / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology
D006657:Histones; D011837:Radiation-Protective Agents; C000594379:monoglucosyl-rutin; D012431:Rutin