Application of rumen microbes to enhance food waste hydrolysis in acidogenic leach-bed reactors.
Effect of rumen microorganisms on hydrolysis of food waste in leach bed reactor (LBR) was investigated. LBRs were inoculated (20%, w/w) with cow manure and anaerobically digested sludge at different ratios, 0:1 (LBR-A), 1:3 (LBR-B), 1:1 (LBR-C), 3:1 (LBR-D) and 1:0 (LBR-E). High volatile solids (VS) conversion efficiency of 68% was achieved in LBR-E. Compared with LBR-A, chemical oxygen demand, total soluble products and total Kjeldahl nitrogen leaching of LBR-E were increased by 16%, 14.3% and 27%, respectively. Recovery of the highest amounts of ethanol and butyrate in LBR-E indicated that the metabolic pathway mediated by rumen microorganisms was favorable for subsequent methanogenesis. Phylogenetic analysis confirmed that the enhanced hydrolysis in LBR-E was mainly due to strong degraders, e.g. Enterobacter, Bifidobacterium thermacidophilum and Caloramator sourced from cow manure. Results demonstrate that rumen microorganisms rapidly degrade the VS and produce useful VFAs with high methane yields in subsequent methanogenesis.
Sino-Forest Applied Research Centre for Pearl River Delta Environment, Department of Biology, Hong Kong Baptist University, Hong Kong Special Administrative Region, PR China.
Bing Hua
BH
Yan
Sino-Forest Applied Research Centre for Pearl River Delta Environment, Department of Biology, Hong Kong Baptist University, Hong Kong Special Administrative Region, PR China.
Ammaiyappan
A
Selvam
Sino-Forest Applied Research Centre for Pearl River Delta Environment, Department of Biology, Hong Kong Baptist University, Hong Kong Special Administrative Region, PR China. Electronic address: jwcwong@hkbu.edu.hk.
Jonathan W C
JW
Wong
D000085:Acetates / Q000378:metabolism; D000143:Acids / Q000378:metabolism*; D064751:Ammonium Compounds / Q000032:analysis; D000818:Animals; D001419:Bacteria / Q000235:genetics; D057919:Biological Oxygen Demand Analysis; D019149:Bioreactors / Q000382:microbiology*; D002087:Butyrates / Q000378:metabolism; D004275:DNA, Ribosomal / Q000235:genetics; D058645:Denaturing Gradient Gel Electrophoresis; D000431:Ethanol / Q000378:metabolism; D005502:Food; D015964:Gene Expression Regulation, Bacterial; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D053858:Metabolic Networks and Pathways; D010802:Phylogeny; D011422:Propionates / Q000378:metabolism; D012037:Refuse Disposal / Q000379:methods*; D012417:Rumen / Q000382:microbiology*; D012995:Solubility; D014866:Waste Products
D000085:Acetates; D000143:Acids; D064751:Ammonium Compounds; D002087:Butyrates; D004275:DNA, Ribosomal; D011422:Propionates; D014866:Waste Products; D000431:Ethanol
Anaerobic hydrolysis; Food waste; Leach bed reactor; Rumen microbes