[HPLC fingerprint and chemical pattern recognition of liposoluble chemical components for euodiae fructus].
OBJECTIVE
To establish a method of HPLC fingerprint and chemical pattern recognition of liposoluble chemical components for quality evaluation of Euodiae Fructus.


METHODS
The HPLC chromatographic separation was performed on a C18 column (250 mm x 4.6 mm, 5 microm). The mobile phase was a mixture of acetonitrile-0.5% acetic acid in gradient elution with flow rate of 1 mL/min. The detection wavelength was set at 249 nm, and the column temperature was 30 degrees C.


RESULTS
There were 9 common peaks in the HPLC fingerprint of 34 batches of Euodiae Fructus samples. The results of cluster analysis and principal component analysis showed that the liposoluble chemical components of Euodia rutaecarpa (Juss.) Benth. var. officinalis (Dode) Huang and Euodia rutaecarpa (Juss.) Benth. var. bodinieri (Dode) Huang were more similar than that of Euodia rutaecarpa (Juss.) Benth.


CONCLUSIONS
The results provide new experimental and theoretical basis for quality control and evaluation of Euodiae Fructus.
Li-Ping
LP
Tang
Juan
J
Wei
Jin-Xiang
JX
Zeng
Ying
Y
Bi
Guang-Ming
GM
Luo
Shou-Wen
SW
Zhang
D002851:Chromatography, High Pressure Liquid; D016000:Cluster Analysis; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D017753:Ecosystem; D032086:Evodia / Q000737:chemistry* / Q000145:classification; D005638:Fruit / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D025341:Principal Component Analysis; D011786:Quality Control
D004365:Drugs, Chinese Herbal