Techniques for slow cryopreservation of embryos.
The slow cryopreservation of embryos has been used for nearly three decades as a means of storing surplus conceptuses from single IVF (in vitro fertilization) cycles. Doing so has allowed caregivers to maximize pregnancy rates without wastage of precious biological materials. Very detailed methods are described here using a popular biological freezing unit manufactured by Planer PLC (Middlesex, UK). Culture media preparation and tranfer protocols, including replacement in both natural and stimulated cycles, are included.
Weill Cornell Medical College, 1300 York Avenue, New York, NY, 10065, USA, lveeckgosden@gmail.com.
Lucinda Veeck
LV
Gosden
D015925:Cryopreservation / Q000379:methods*; D003470:Culture Media; D004624:Embryo Transfer / Q000379:methods; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D008967:Molecular Biology / Q000379:methods; D011247:Pregnancy
D003470:Culture Media