Lectin-tagged fluorescent polymeric nanoparticles for targeting of sialic acid on living cells.
In this study, we fabricated lectin-tagged fluorescent polymeric nanoparticles approximately 35 nm in diameter using biocompatible polymers conjugated with lectins for the purpose of detecting sialic acid on a living cell surface, which is one of the most important biomarkers for cancer diagnosis. Through cellular experiments, we successfully detected sialic acid overexpression on cancerous cells with high specificity. These fluorescent polymeric nanoparticles can be useful as a potential bioimaging probe for detecting diseased cells.
Department of Bioengineering, Grad10-ate School of Engineering, The University of Tokyo , Hongo 7-3-1, Bunkyo-ku, Tokyo 113-8656, Japan.
Jaebum
J
Cho
Keiichiro
K
Kushiro
Yuji
Y
Teramura
Madoka
M
Takai
D000818:Animals; D002462:Cell Membrane / Q000187:drug effects* / Q000378:metabolism; D016503:Drug Delivery Systems / Q000379:methods*; D005456:Fluorescent Dyes / Q000008:administration & dosage* / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D061986:MCF-7 Cells; D051379:Mice; D019158:N-Acetylneuraminic Acid / Q000378:metabolism; D053758:Nanoparticles / Q000008:administration & dosage* / Q000378:metabolism; D024301:Plant Bark; D010936:Plant Extracts / Q000008:administration & dosage / Q000378:metabolism; D037121:Plant Lectins / Q000008:administration & dosage* / Q000378:metabolism; D011108:Polymers / Q000008:administration & dosage* / Q000378:metabolism; D027924:Sambucus nigra
D005456:Fluorescent Dyes; D010936:Plant Extracts; D037121:Plant Lectins; D011108:Polymers; D019158:N-Acetylneuraminic Acid