Comparative molecular analysis of bacterial communities inhabiting pristine and polluted with polycyclic aromatic hydrocarbons Black Sea coastal sediments.
Molecular analysis was applied to characterize bacterial community structure in sediment samples collected from pristine site and oil-polluted Black Sea harbor. Amplified Ribosomal DNA Restriction Analysis (ARDRA) revealed a high similarity in the restriction patterns of both samples thus not demonstrating the effect of the pollutant on the structure of the bacterial communities. Constructed 16S rRNA gene libraries gave more detailed assessment of members. Results showed that α- and γ-Proteobacteria were dominant in the oil polluted site, whereas the pristine site was characterized by prevalence of Actinobacteria. The biodegradative potential of the adapted bacterial community in the oil-polluted sediments was demonstrated by the presence of the aromatic ring hydroxylating dioxygenase genes.
Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, Y. Gagarin 2 str., 1113 Sofia, Bulgaria. Electronic address: nadeshda@abv.bg.
Nadezhda H
NH
Todorova
Institute of Molecular Biology "Roumen Tsanev", Bulgarian Academy of Sciences, G. Bonchev str., bl. 21, 1113 Sofia, Bulgaria. Electronic address: rumym@bio21.bas.bg.
Roumyana S
RS
Mironova
Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, Y. Gagarin 2 str., 1113 Sofia, Bulgaria. Electronic address: karamfilov.v@gmail.com.
Ventzislav K
VK
Karamfilov
D001419:Bacteria / Q000145:classification / Q000378:metabolism; D059038:Black Sea; D015723:Gene Library; D019015:Geologic Sediments / Q000737:chemistry / Q000382:microbiology*; D064307:Microbiota; D059392:Petroleum Pollution; D010802:Phylogeny; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA
D011084:Polycyclic Aromatic Hydrocarbons; D012336:RNA, Ribosomal, 16S
16S rRNA gene; ARDRA; Bacterial communities; Oil pollution; PAH degradative potential; Sediment