[High performance preparation and structural confirmation of lignans from Schisandrae chinensis fructus by using HSCCC combined with ESI-MSn method].
High-speed counter-current chromatography (HSCCC) was used to high performance separate and prepare lignans from Schisandrae chinensis fructus. The solvent system is composed of n-hexane-ethyl acetate-methanol-water (9 : 1 : 5 : 5) and n-hexane-ethyl acetate-methanol-water (9 : 1 : 9 : 5), speed is at 900 r.min-1, and flow rate is at 2.0 mL.min-1. Five fractions from Schisandrae chinensis fructus extract were separated and prepared with one HSCCC process. They were identified as schisandrin, gomisin J, schisandrol B, schisantherin A and deoxyschizandrin by electrospray ionization-multiple tandem mass spectrometry (ESI-MSn), respectively. Their contents were obtained in 98.74%, 94.32%, 99.53%, 94.23% and 98.68% by ultra high performance liquid chromatography (UPLC), separately. The rapid and simple method can be applied for the preparation of lignans from Schisandrae chinensis fructus.
Xiao-Li
XL
Yu
Zi-feng
ZF
Pi
Xiu-Li
XL
Hu
Feng-Rui
FR
Song
Zhi-Qiang
ZQ
Liu
D003377:Countercurrent Distribution; D034242:Cyclooctanes / Q000737:chemistry / Q000302:isolation & purification*; D004149:Dioxoles / Q000737:chemistry / Q000302:isolation & purification*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification; D005638:Fruit / Q000737:chemistry; D017705:Lignans / Q000737:chemistry / Q000302:isolation & purification*; D015394:Molecular Structure; D010946:Plants, Medicinal / Q000737:chemistry; D011083:Polycyclic Compounds / Q000737:chemistry / Q000302:isolation & purification*; D032141:Schisandra / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
D034242:Cyclooctanes; D004149:Dioxoles; D004365:Drugs, Chinese Herbal; D017705:Lignans; D011083:Polycyclic Compounds; C033585:schizandrol B; C034734:schizandrin A; C034557:schizandrer A; C011105:schizandrin; C082091:gomisin J