Chitosan-coated electrospun PLA fibers for rapid mineralization of calcium phosphate.
In this work, hydroxyapatite (HA) mineralized on chitosan (CS)-coated poly(lactic acid) (PLA) nanofiber mat was prepared and compared in terms of mineralization characteristics. Significant calcium phosphate crystals formed on various concentrations of CS-coated PLA fiber mat with better uniformity after 2h of incubation in 10 times simulated body fluid (10× SBF). X-ray diffraction results further indicated that the composition of the deposited mineral was a mixture of dicalcium phosphate dehydrates and apatite. Chitosan, a cationic polysaccharide, can promote more nucleation and growth of calcium phosphate under conditions of 0.4% chitosan concentrations. These results indicated that HA-mineralized on CS-coated PLA fiber mat can be prepared directly via simply using CS coating followed by SBF immersion, and the results also suggest that this composite can mimic structural, compositional, and biological functions of native bone and can serve as a good candidate for bone tissue engineering (BTE).
Department of Chemical and Materials Engineering, Tunghai University, Taiwan. Electronic address: chichang31@thu.edu.tw.
Chi-Chang
CC
Lin
Department of Chemical and Materials Engineering, Tunghai University, Taiwan.
Shu-Juan
SJ
Fu
Department of Chemical and Materials Engineering, Tunghai University, Taiwan.
Yu-Ching
YC
Lin
Department of Chemical and Materials Engineering, Tunghai University, Taiwan. Electronic address: ikyang@thu.edu.twy.
I-Kuan
IK
Yang
Department of Chemical and Materials Engineering, Tunghai University, Taiwan. Electronic address: yegu@thu.edu.tw.
Yesong
Y
Gu
D000469:Alkaline Phosphatase / Q000378:metabolism; D002130:Calcium Phosphates / Q000737:chemistry*; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D048271:Chitosan / Q000494:pharmacology*; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D003460:Crystallization; D017886:Durapatite / Q000494:pharmacology; D057075:Enzyme Assays; D006801:Humans; D019344:Lactic Acid / Q000494:pharmacology*; D008903:Minerals / Q000737:chemistry*; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure; D011091:Polyesters; D011108:Polymers / Q000494:pharmacology*; D013052:Spectrometry, X-Ray Emission; D017550:Spectroscopy, Fourier Transform Infrared; D013059:Spectrum Analysis, Raman; D023822:Tissue Engineering / Q000379:methods*; D014961:X-Ray Diffraction
D002130:Calcium Phosphates; D020099:Coated Materials, Biocompatible; D008903:Minerals; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide); D048271:Chitosan; D017886:Durapatite; C020243:calcium phosphate; D000469:Alkaline Phosphatase
Chitosan; Electrospun fiber; Simulated body fluid