Corticotropin-releasing factor-binding protein (CRF-BP) inhibits CRF- and urotensin-I-mediated activation of CRF receptor-1 and -2 in common carp.
Corticotropin-releasing factor-binding protein (CRF-BP) is considered a key determinant for CRF receptor (CRF-R) activation by CRF and several related peptides. Earlier studies have shown that the CRF system is highly conserved in gene structures throughout evolution, yet little is known about the evolutionary conservation of its biological functions. Therefore, we address the functional properties of CRF-BP and CRF-Rs in a teleost fish (common carp; Cyprinus carpio L.). We report the finding of two similar, yet distinct, genes for both CRF-R1 and CRF-R2 in this species. The four receptors are differentially responsive to CRF, urotensin-I (UI), sauvagine, and urocortin-2 (Ucn-2) and -3 (Ucn-3) as shown by luciferase assays. In vitro, carp CRF-BP inhibits CRF- and UI-mediated activation of the newfound CRF-Rs, but its potency to do so varies between receptor and peptide ligand. This is the first paper to establish the functionality and physiological interplay between CRF-BP, CRF-Rs and CRF-family peptides in a teleostean species.
Department of Organismal Animal Physiology, Institute for Water and Wetland Research, Radboud University Nijmegen, Heyendaalseweg 135, 6525 AJ Nijmegen, The Netherlands. Electronic address: r.manuel@science.ru.nl.
Remy
R
Manuel
Department of Organismal Animal Physiology, Institute for Water and Wetland Research, Radboud University Nijmegen, Heyendaalseweg 135, 6525 AJ Nijmegen, The Netherlands.
Juriaan R
JR
Metz
Department of Organismal Animal Physiology, Institute for Water and Wetland Research, Radboud University Nijmegen, Heyendaalseweg 135, 6525 AJ Nijmegen, The Netherlands.
Gert
G
Flik
The Salk Institute for Biological Studies, Clayton Foundation Laboratories for Peptide Biology, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA.
Wylie W
WW
Vale
The Salk Institute for Biological Studies, Clayton Foundation Laboratories for Peptide Biology, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA.
Mark O
MO
Huising
D029845:Amphibian Proteins / Q000378:metabolism; D000818:Animals; D002347:Carps / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D003346:Corticotropin-Releasing Hormone / Q000378:metabolism*; D000242:Cyclic AMP / Q000494:pharmacology; D057809:HEK293 Cells; D006801:Humans; D008156:Luciferases / Q000378:metabolism; D036361:Peptide Hormones / Q000378:metabolism; D020033:Protein Isoforms / Q000378:metabolism; D018019:Receptors, Corticotropin-Releasing Hormone / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D054832:Urocortins / Q000378:metabolism; D014579:Urotensins / Q000378:metabolism*
D029845:Amphibian Proteins; D002352:Carrier Proteins; D036361:Peptide Hormones; D020033:Protein Isoforms; D018019:Receptors, Corticotropin-Releasing Hormone; D011994:Recombinant Proteins; D054832:Urocortins; D014579:Urotensins; C067133:corticotropin releasing factor-binding protein; C025727:sauvagine; D003346:Corticotropin-Releasing Hormone; C029676:urotensin I; D000242:Cyclic AMP; D008156:Luciferases
CRF; CRF-BP; CRF-R; Common carp