Evaluation of ZAR1 and SFRP4 methylation status as potentials biomarkers for diagnosis in cervical cancer: exploratory study phase I.
BACKGROUND
Aberrant hypermethylation of promoter region of tumor suppressor genes could be used as cancer biomarkers.


OBJECTIVE
To test methylation status of ZAR1 and SFRP4 promoter regions as potentials biomarkers for diagnosis of preneoplastic and neoplastic lesions of cervix.


METHODS
Cytobrush samples were evaluated by Methylation specific PCR (MSP) and quantitative MSP (qMSP).


RESULTS
ZAR1 and SFRP4 methylation frequency increased as the grade of lesion increased and the differences between normal and cervical cancer (CC) are statistically significant (p < 0.0001). qMSP showed higher ZAR1 and SFRP4 methylation levels in cancer than normal epithelia (p < 0.001) and preneoplastics lesions (p < 0.01).


CONCLUSIONS
qMSP quantify methylation levels and have high sensitivity and specificity.


CONCLUSIONS
ZAR1 and SFRP4 qMSP could be used as potential biomarker for CC diagnosis.
Molecular Pathology Laboratory, Department of Pathology .
Priscilla
P
Brebi
Rene
R
Hoffstetter
Alejandra
A
Andana
Carmen G
CG
Ili
Kathleen
K
Saavedra
Tamara
T
Viscarra
Javier
J
Retamal
Raul
R
Sanchez
Juan C
JC
Roa
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000235:genetics*; D019175:DNA Methylation; D017931:DNA Primers; D004527:Egg Proteins / Q000235:genetics*; D005260:Female; D006801:Humans; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis* / Q000235:genetics; D055815:Young Adult; D000094742:Secreted Frizzled-Related Proteins
D014408:Biomarkers, Tumor; D017931:DNA Primers; D004527:Egg Proteins; D011518:Proto-Oncogene Proteins; C118723:SFRP4 protein, human; D000094742:Secreted Frizzled-Related Proteins; C471631:Zar1 protein, human