Characterization of limes (Citrus aurantifolia) grown in Bhutan and Indonesia using high-throughput sequencing.
Lime [Citrus aurantifolia (Cristm.) Swingle] is a Citrus species that is a popular ingredient in many cuisines. Some citrus plants are known to originate in the area ranging from northeastern India to southwestern China. In the current study, we characterized and compared limes grown in Bhutan (n = 5 accessions) and Indonesia (n = 3 accessions). The limes were separated into two groups based on their morphology. Restriction site-associated DNA sequencing (RAD-seq) separated the eight accessions into two clusters. One cluster contained four accessions from Bhutan, whereas the other cluster contained one accession from Bhutan and the three accessions from Indonesia. This genetic classification supported the morphological classification of limes. The analysis suggests that the properties associated with asexual reproduction, and somatic homologous recombination, have contributed to the genetic diversification of limes.
1] Department of Applied Biological Sciences, Saga University, Honjo, Saga, Japan [2] Renewable Natural Resources Research Centre Wengkhar, Mongar, Bhutan.
Tshering
T
Penjor
Department of Applied Biological Sciences, Saga University, Honjo, Saga, Japan.
Takashi
T
Mimura
Department of Applied Biological Sciences, Saga University, Honjo, Saga, Japan.
Ryoji
R
Matsumoto
1] Faculty of Agriculture, Kagoshima University, Korimoto, Kagoshima, Japan [2].
Masashi
M
Yamamoto
1] Analytical Research Center for Experimental Sciences, Saga University, Honjo, Saga, Japan [2].
Yukio
Y
Nagano
D001631:Bhutan; D032082:Citrus aurantiifolia / Q000033:anatomy & histology / Q000145:classification* / Q000235:genetics*; D014644:Genetic Variation; D059014:High-Throughput Nucleotide Sequencing; D007214:Indonesia; D017422:Sequence Analysis, DNA