[Anti-tumor effect of 5-FU-PLLA-CNTs on human gastric carcinoma cell lines in vitro].
OBJECTIVE
To prepare cisPLLAtin-loaded polylactic acid/cnts, and to study the anti-tumor effect of 5-FU-PLLA-CNTs on human gastric carcinoma cell lines(MGC803 and MNK45).


METHODS
5-FU-PLLA-CNTs were prepared with ultrasound emulsification. The morphology of 5-FU-PLLA-CNTs was determined by scanning electron microscope(SEM), and its drug loading and drug release curve in vitro were detected by UV-Vis-NIR spectrophotometer. Cells were divided into experiment, positive control and negative control groups. CCK8 method was used to test the cytotoxic effect of 5-FU-PLLA-CNTs in different concentrations on MGC803 and MNK45 cell proliferation. Flow cytometry was employed to measure the apoptotic rate of MGC803 and MNK45 cells before and after the intervention of 5-FU-PLLA-CNTs.


RESULTS
Deep layer film of 5-FU-PLLA-CNTs was successfully established, whose drug-load rate was(4.54±0.43)%, entrapment rate was(21.56±2.36)%. In vitro release test showed release rate within 24 h of 5-FU-PLLA-CNTs was 23.9% in a as lowly increasing manner, and accumulating release rate was 85.3% at day 31. CCk8 experiment revealed, as compared to control group, 5-FU-PLLA-CNTs significantly inhibited the proliferation of two cell lines in dose-dependent and time-dependent manner. The best 5-FU-PLLA-CNTs concentration of inhibition for human gastric cancer cell lines was 1 mg/well. Flow cytometry indicated the apoptotic rate of MGC803 and MNK45 cells in experiment group treated by 1 mg/well 5-FU-PLLA-CNTs significantly increased as compared to negative control group (P<0.05), while the difference was not significant as compared to positive control group (P>0.05).


CONCLUSIONS
The 5-FU-PLLA-CNTs has good drug sustained-release capacity, and can significantly kill and inhibit the proliferation of MGC803 and MNK45 cell lines.
Research Institute of Biliary Tract Disease, Laboratory of General Surgery and Department of General Surgery, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China. laoniulyb@163.com.
Jun
J
Gu
Maolan
M
Li
Xiangsong
X
Wu
Wenguang
W
Wu
Lin
L
Zhang
Qichen
Q
Ding
Jiahua
J
Yang
Hao
H
Weng
Qian
Q
Ding
Runfa
R
Bao
Yijun
Y
Shu
Yingbin
Y
Liu
D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003692:Delayed-Action Preparations; D005472:Fluorouracil / Q000494:pharmacology*; D006801:Humans; D019344:Lactic Acid / Q000494:pharmacology*; D037742:Nanotubes, Carbon; D011091:Polyesters; D011108:Polymers / Q000494:pharmacology*; D013274:Stomach Neoplasms / Q000473:pathology*
D003692:Delayed-Action Preparations; D037742:Nanotubes, Carbon; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide); D005472:Fluorouracil