Speciation analysis of orthophosphate and myo-inositol hexakisphosphate in soil- and plant-related samples by high-performance ion chromatography combined with inductively coupled plasma mass spectrometry.
A novel method based on high-performance ion chromatography inductively coupled plasma mass spectrometry employing strong anion exchange chromatography with HNO3 gradient elution for simultaneous analysis of orthophosphate and myo-inositol hexakisphosphate (IP6 ) in soil solution and plant extracts is presented. As inductively coupled plasma mass spectrometry analysis of phosphorus at m/z 31 is hampered by N-based interferences, (31)P was measured as (31)P(16)O(+) at m/z 47 employing dynamic reaction cell technique with O2 as reaction gas. Orthophosphate and IP6 were separated within a total chromatographic run-time of 12 min revealing a limit of detection of 0.3 μmol/L. The coefficients of determination obtained in a working range of 1-100 and 1-30 μmol/L were 0.9991 for orthophosphate and 0.9968 for IP6, respectively. The method was successfully applied to extracts from three different soils as well as root and shoot extracts of Brassica napus L. The precision of three independently prepared soil extracts was in the range of 4-10% relative standard deviation for PO4 (3-) and 3-8% relative standard deviation for IP6. Soil adsorption/desorption kinetics for IP6/orthophosphate were performed for investigating the sorption behavior of the two P species in the experimental soils.
Division of Analytical Chemistry, Department of Chemistry, University of Natural Resources and Life Sciences-BOKU, Vienna, Austria.
Ariana
A
Rugova
Markus
M
Puschenreiter
Jakob
J
Santner
Lisa
L
Fischer
Stefan
S
Neubauer
Gunda
G
Koellensperger
Stephan
S
Hann
D029688:Brassica napus / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007294:Inositol / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification; D013058:Mass Spectrometry / Q000379:methods*; D015394:Molecular Structure; D010710:Phosphates / Q000737:chemistry* / Q000302:isolation & purification; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification; D018517:Plant Roots / Q000737:chemistry; D018520:Plant Shoots / Q000737:chemistry; D010944:Plants / Q000737:chemistry*; D012987:Soil / Q000737:chemistry*
D010710:Phosphates; D010936:Plant Extracts; D012987:Soil; C013063:inositol hexasulfate; D007294:Inositol
Orthophosphate; Phytic acid; Plant extracts; Soil adsorption; Soil extracts