Improvement of beta-galactosidase immobilization and its application to ethanol fermentation of lactose.
To intensify ethanol fermentation of lactose, a co-immobilized biocatalyzer was used. It consisted of yeast cells of Saccharomyces cerevisiae co-immobilized with beta-galactosidase preparation from A. oryzae. The experiment was focused on the improvement of biocatalyzer operational stability. An increase in the concentration of a carrier (2% calcium alginate) did not improve the effectiveness of binding beta-galactosidase. However, its initial cross-linking with glutaraldehyde and its co-immobilization with distillery industrial yeast produced a stable biocatalyzer able to ferment mash from permeate (12% lactose) with an average efficiency of 6.5% v/v of alcohol during 10 experimental series (30 days).
M
M
Lewandowska
W
W
Bednarski
S
S
Kulesza
D000464:Alginates / Q000737:chemistry; D001236:Aspergillus oryzae / Q000201:enzymology*; D004800:Enzymes, Immobilized / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D007218:Industrial Microbiology / Q000379:methods*; D007785:Lactose / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D001616:beta-Galactosidase / Q000378:metabolism*
D000464:Alginates; D004800:Enzymes, Immobilized; D006603:Hexuronic Acids; D000431:Ethanol; D020723:Glucuronic Acid; D001616:beta-Galactosidase; D007785:Lactose