[Preparation of tranexamic acid-loaded porous starch and evaluation of its hemostatic ability].
This study was aimed to develop a new generation of ideal hemostatic powder which can be safely, effectively and easily used mainly to first aid anterior to hospital by the synergistic effect of physical and chemical hemostatic mechanisms. The tranexamic acid(TA)-loaded porous starch(PS) (TAPS) was prepared by using PS as carrier and TA as loaded drug component. The absorption property of TAPS was evaluated by water absorption; the hemostatic ability of TAPS was evaluated by test in vitro and in vivo, the blood coagulation time of TAPS was detected by using Lee-white method. The experiment was divided into 3 groups: blank control group, Yunnan Baiyao group and TAPS group, each group with 10 blood samples in vitro test; the 27 SD rats were used to test in vivo, and randomly were divided into 3 groups: PS,Yunnan Baiyao and TAPS, each group consisted of 9 rats for establishing the animal model of liver trauma and detecting the complete hemostasis time. The results showed that the water absorption of PS did not be affected by TA when dose of TA loaded in PS was <0.02 g/g PS. There was no statistic difference in blood coagulation time between TAPS and PS groups(P > 0.05). The complete hemostatic time of TAPS for trauma of left lobe liver was 236.67 ± 55.00 seconds, which was shorter than that of Yunnan Baiyao (340.00 ± 73.48 seconds) and PS (396.67 ± 68.37 seconds) (P < 0.05 and P < 0.01, respectively). It is concluded that PS can load TA and play the hemostatic effect through releasing TA; the TA loading <0.02 g/g PS did not affect the water absorption and pro-coagulation properties. The TA can enhance the hemostatic efficacy of PS, the hemostatic property of TAPS is derived from synergism of physical and chemical hemostatic mechanisms.
Department of Blood Transfusion, Chinese PLA General Hospital, Beijing 100853,China.
Chao-Yun
CY
Xi
Department of Blood Transfusion, Chinese PLA General Hospital, Beijing 100853,China.
Yuan
Y
Zhuang
Department of Blood Transfusion, Chinese PLA General Hospital, Beijing 100853,China.
Lin Feng
LF
Chen
Department of Blood Transfusion, Chinese PLA General Hospital, Beijing 100853,China.
Ya-Qian
YQ
Liu
Department of Blood Transfusion, Chinese PLA General Hospital, Beijing 100853,China.
Shu-Fang
SF
Wang
Department of Blood Transfusion, Chinese PLA General Hospital, Beijing 100853, China. E-mail: deqingw@vip.sina.com.
De-Qing
DQ
Wang
D000818:Animals; D001780:Blood Coagulation Tests; D004337:Drug Carriers; D006490:Hemostatics / Q000138:chemical synthesis* / Q000494:pharmacology; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013213:Starch / Q000008:administration & dosage; D014148:Tranexamic Acid / Q000138:chemical synthesis* / Q000494:pharmacology
D004337:Drug Carriers; D006490:Hemostatics; D014148:Tranexamic Acid; D013213:Starch