Trans-anethole protects cortical neuronal cells against oxygen-glucose deprivation/reoxygenation.
Trans-anethole has been studied on pharmacological properties such as anti-inflammation, anti-oxidative stress, antifungal and anticancer. However, to date, the anti-ischemic effects of trans-anethole have not been assessed. Therefore, we investigated the neuroprotection of trans-anethole against oxygen-glucose deprivation/reoxygenation (OGD/R)-induced cortical neuronal cell injury, an in vitro model of ischemia. The abilities of trans-anethole to block excitotoxicity, oxidative stress and mitochondrial dysfunction were evaluated in OGD/R-induced neurons. Trans-anethole significantly ameliorated OGD/R-induced neuronal cell injury by attenuating the intracellular calcium overload via the activation of NMDA receptors. Trans-anethole also inhibited OGD/R-induced reactive oxygen species overproduction, which may be derived from the scavenging activity in peroxyl radicals, assessed in an oxygen radical absorbance capacity assay. Furthermore, trans-anethole was shown to attenuate the depolarization of mitochondrial transmembrane. These results indicated that the neuroprotective effect of trans-anethole on OGD/R-induced neuronal injury might be due to its ability to inhibit excitotoxicity, oxidative stress and mitochondrial dysfunction. Considering these multiple pathways causing ischemic neuronal damage, the multi-functional effect of trans-anethole suggested that it may be effective in treating ischemic stroke.
Department of Neuroscience, School of Medicine, Korea University, Seoul, 136-705, Republic of Korea.
Sangwoo
S
Ryu
Geun Hee
GH
Seol
Hyeon
H
Park
In-Young
IY
Choi
D000081248:Allylbenzene Derivatives; D000818:Animals; D000840:Anisoles / Q000008:administration & dosage*; D002118:Calcium / Q000378:metabolism; D016923:Cell Death / Q000187:drug effects; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000187:drug effects*; D005947:Glucose / Q000378:metabolism; D020925:Hypoxia-Ischemia, Brain / Q000188:drug therapy*; D009474:Neurons / Q000187:drug effects*; D018696:Neuroprotective Agents / Q000008:administration & dosage*; D010100:Oxygen / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species
D000081248:Allylbenzene Derivatives; D000840:Anisoles; D018696:Neuroprotective Agents; D017382:Reactive Oxygen Species; D005947:Glucose; C006578:anethole; D010100:Oxygen; D002118:Calcium