Selective identification and quantification of saccharin by liquid chromatography and fluorescence detection.
High-pressure liquid chromatography with ultra-violet detection (HPLC-UV) is one of the most commonly used methods to identify and quantify saccharin in non-alcoholic beverages. However, due to the wide variety of interfering UV spectra in saccharin-containing beverage matrices, the same method cannot be used to measure this analyte accurately. We have developed a new, highly effective method to identify and quantify saccharin using HPLC with fluorescence detection (HPLC-FLD). The excitation wavelength (250 nm) and emission wavelength (440 nm) chosen increased selectivity for all matrices and ensured few changes were required in the mobile phase or other parameters. The presence of saccharin in non-diet beverages - a fraud commonly used to replace more expensive sucrose - was confirmed by comparing coincident peaks as well as the emission spectra of standards and samples.
Advanced Laboratory Service - SLAV-RJ/LANAGRO-MG/MAPA, Av. Maracanã, 252, CEP 20271-110 Rio de Janeiro-RJ, Brazil. Electronic address: sergionfbruno@gmail.com.
Sergio N F
SN
Bruno
Advanced Laboratory Service - SLAV-RJ/LANAGRO-MG/MAPA, Av. Maracanã, 252, CEP 20271-110 Rio de Janeiro-RJ, Brazil.
Carlos R
CR
Cardoso
Advanced Laboratory Service - SLAV-RJ/LANAGRO-MG/MAPA, Av. Maracanã, 252, CEP 20271-110 Rio de Janeiro-RJ, Brazil.
Márcia Mosca A
MM
Maciel
Advanced Laboratory Service - SLAV-RJ/LANAGRO-MG/MAPA, Av. Maracanã, 252, CEP 20271-110 Rio de Janeiro-RJ, Brazil; Instituto Federal do Rio de Janeiro (IFRJ), Campus Rio de Janeiro, Av. Senador Furtado, 121, CEP 20270-021 Rio de Janeiro-RJ, Brazil.
Lidmila
L
Vokac
Instituto Federal do Rio de Janeiro (IFRJ), Campus Rio de Janeiro, Av. Senador Furtado, 121, CEP 20270-021 Rio de Janeiro-RJ, Brazil.
Ademário I
AI
da Silva Junior
D001628:Beverages / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D002853:Chromatography, Liquid; D005503:Food Additives / Q000032:analysis*; D012439:Saccharin / Q000737:chemistry*
D005503:Food Additives; D012439:Saccharin
Fluorescence detection in liquid chromatography; Saccharin fraud; Selective saccharin determination