Ionic liquid-anionic surfactant based aqueous two-phase extraction for determination of antibiotics in honey by high-performance liquid chromatography.
An ionic liquid-anionic surfactant based aqueous two-phase extraction was developed and applied for the extraction of tetracycline, oxytetracycline and chloramphenicol in honey. The honey sample was mixed with Na2EDTA aqueous solution. The sodium dodecyl sulfate, ionic liquid 1-octyl-3-methylimidazolium bromide and sodium chloride were added in the mixture. After the resulting mixture was ultrasonically shaken and centrifuged, the aqueous two phase system was formed and analytes were extracted into the upper phase. The parameters affecting the extraction efficiency, such as the volume of ionic liquid, the category and amount of salts, sample pH value, extraction time and temperature were investigated. The limits of detection of tetracycline, oxytetracycline and chloramphenicol were 5.8, 8.2 and 4.2 μg kg(-1), respectively. When the present method was applied to the analysis of real honey samples, the recoveries of analytes ranged from 85.5 to 110.9% and relative standard deviations were lower than 6.9%.
College of Chemistry, Jilin University, Qianjin Street 2699, Changchun 130012, PR China.
Xiao
X
Yang
Department of Rehabilitation, the General Hospital of FAW (the Fourth Hospital of Jilin University), Haoyue Road 3788, Changchun 130011, PR China.
Shaohua
S
Zhang
Department of Hand Surgery, China-Japan Union Hospital, Jilin University, Xiantai Street 126, Changchun 130033, PR China.
Wei
W
Yu
College of Chemistry, Jilin University, Qianjin Street 2699, Changchun 130012, PR China.
Zhongling
Z
Liu
College of Chemistry, Jilin University, Qianjin Street 2699, Changchun 130012, PR China.
Lei
L
Lei
College of Chemistry, Jilin University, Qianjin Street 2699, Changchun 130012, PR China.
Na
N
Li
College of Chemistry, Jilin University, Qianjin Street 2699, Changchun 130012, PR China.
Hanqi
H
Zhang
College of Instrumentation and Electrical Engineering, Jilin University, Qianjin Street 2699, Changchun 130012, PR China. Electronic address: vivianyxiao@163.com.
Yong
Y
Yu
D000838:Anions / Q000737:chemistry; D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D001965:Bromides / Q000737:chemistry; D002701:Chloramphenicol / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004492:Edetic Acid / Q000737:chemistry; D006722:Honey / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D007093:Imidazoles / Q000737:chemistry; D052578:Ionic Liquids / Q000737:chemistry*; D059627:Liquid Phase Microextraction / Q000379:methods*; D015394:Molecular Structure; D010118:Oxytetracycline / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D015203:Reproducibility of Results; D012965:Sodium Chloride / Q000737:chemistry; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D013501:Surface-Active Agents / Q000737:chemistry*; D013752:Tetracycline / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D013997:Time Factors
C528768:1-octyl-3-methylimidazolium; D000838:Anions; D000900:Anti-Bacterial Agents; D001965:Bromides; D007093:Imidazoles; D052578:Ionic Liquids; D013501:Surface-Active Agents; D012967:Sodium Dodecyl Sulfate; D012965:Sodium Chloride; D002701:Chloramphenicol; D004492:Edetic Acid; D013752:Tetracycline; D010118:Oxytetracycline
Anionic surfactant; Antibiotics; Aqueous two-phase extraction; Honey; Ionic liquid