Autoregulation of the Kluyveromyces lactis pyruvate decarboxylase gene KlPDC1 involves the regulatory gene RAG3.
In the yeast Kluyveromyces lactis, the pyruvate decarboxylase gene KlPDC1 is strongly regulated at the transcription level by different environmental factors. Sugars and hypoxia act as inducers of transcription, while ethanol acts as a repressor. Their effects are mediated by gene products, some of which have been characterized. KlPDC1 transcription is also strongly repressed by its product--KlPdc1--through a mechanism called autoregulation. We performed a genetic screen that allowed us to select and identify the regulatory gene RAG3 as a major factor in the transcriptional activity of the KlPDC1 promoter in the absence of the KlPdc1 protein, i.e. in the autoregulatory mechanism. We also showed that the two proteins Rag3 and KlPdc1 interact, co-localize in the cell and that KlPdc1 may control Rag3 nuclear localization.
Dip. Biologia e Biotecnologie 'Charles Darwin', Sapienza Università di Roma, p.le Aldo Moro 5, 00185 Roma, Italy.
Daniela
D
Ottaviano
Dip. Biologia e Biotecnologie 'Charles Darwin', Sapienza Università di Roma, p.le Aldo Moro 5, 00185 Roma, Italy.
Chiara
C
Micolonghi
Dip. Biologia e Biotecnologie 'Charles Darwin', Sapienza Università di Roma, p.le Aldo Moro 5, 00185 Roma, Italy.
Lorenza
L
Tizzani
CNRS, Villeurbanne, France.|Université Lyon1, Lyon, France.|Génétique Moléculaire des Levures, UMR5240 Microbiologie, Adaptation et Pathogénie, Université de Lyon, Lyon, France.
Marc
M
Lemaire
CNRS, Villeurbanne, France.|Université Lyon1, Lyon, France.|Génétique Moléculaire des Levures, UMR5240 Microbiologie, Adaptation et Pathogénie, Université de Lyon, Lyon, France.
Micheline
M
Wésolowski-Louvel
Istituto Pasteur Fondazione Cenci-Bolognetti, Sapienza Università di Roma, Roma, Italy.|Dip. Biologia e Biotecnologie 'Charles Darwin', Sapienza Università di Roma, p.le Aldo Moro 5, 00185 Roma, Italy.
Maria Egle
ME
De Stefano
Dip. Medicina clinica e molecolare, Sapienza Università di Roma, via di Grottarossa 1035, 00189 Roma, Italy.
Danilo
D
Ranieri
Istituto Pasteur Fondazione Cenci-Bolognetti, Sapienza Università di Roma, Roma, Italy.|Dip. Biologia e Biotecnologie 'Charles Darwin', Sapienza Università di Roma, p.le Aldo Moro 5, 00185 Roma, Italy.
Michele M
MM
Bianchi
D000483:Alleles; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D017930:Genes, Reporter; D005838:Genotype; D006706:Homeostasis / Q000235:genetics*; D007716:Kluyveromyces / Q000201:enzymology* / Q000235:genetics / Q000648:ultrastructure; D008954:Models, Biological; D011401:Promoter Regions, Genetic / Q000235:genetics; D025941:Protein Interaction Mapping; D011767:Pyruvate Decarboxylase / Q000235:genetics* / Q000378:metabolism; D017384:Sequence Deletion; D014158:Transcription, Genetic
D005656:Fungal Proteins; C103819:RAG3 protein, Kluyveromyces lactis; D011767:Pyruvate Decarboxylase