PLCE1 suppresses p53 expression in esophageal cancer cells.
The apoptotic mechanism dysfunction plays a critical role in cancer cell growth and escaping from cancer therapies; the underlying mechanisms are to be further elucidated. This study aims to investigate the role of phospholipase C epsilon 1 (PLCE1) in modulating the apoptosis mechanism in esophageal cancer (Eca) cells. The results showed that Eca cell lines, OE33 and CP-C cells expressed high levels of PLCE1. Knockdown of PLCE1 markedly increased 9.26 folds of the expression of p53 and 13.8 folds of the frequency of apoptotic CP-C cells via modulating the p53 promoter methylation.
Department of Cardiothoracic Surgery, The Third Affiliated Hospital of Sun Yat-sen University , Guangzhou , China.
Yun
Y
Li
Jun
J
An
Shaohong
S
Huang
Hongying
H
Liao
Yimin
Y
Weng
Songwang
S
Cai
Junhang
J
Zhang
D017209:Apoptosis / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D019175:DNA Methylation / Q000235:genetics; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D054801:Phosphoinositide Phospholipase C / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis* / Q000235:genetics
C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; D054801:Phosphoinositide Phospholipase C; C423320:phospholipase C epsilon
Apoptosis; Esophageal cancer; P53; Phospholipase C epsilon 1; Signal transducer and activator of transcription 3