RNA sequencing reveals upregulation of RUNX1-RUNX1T1 gene signatures in clear cell renal cell carcinoma.
In the past few years, therapies targeted at the von Hippel-Lindau (VHL) and hypoxia-inducible factor (HIF) pathways, such as sunitinib and sorafenib, have been developed to treat clear cell renal cell carcinoma (ccRCC). However, the majority of patients will eventually show resistance to antiangiogenesis therapies. The purpose of our study was to identify novel pathways that could be potentially used as targets for new therapies. Whole transcriptome sequencing (RNA-Seq) was conducted on eight matched tumor and adjacent normal tissue samples. A novel RUNX1-RUNX1T1 pathway was identified which was upregulated in ccRCC through gene set enrichment analysis (GSEA). We also confirmed the findings based on previously published gene expression microarray data. Our data shows that upregulated of the RUNX1-RUNX1T1 gene set maybe an important factor contributing to the etiology of ccRCC.
Department of Urology, Huashan Hospital, Fudan University, 12 Central Urumqi Road, Shanghai 200040, China.
Zuquan
Z
Xiong
Fudan-VARI Center for Genetic Epidemiology, School of Life Sciences, Fudan University, Shanghai 200433, China.
Hongjie
H
Yu
Laboratory of Bioinformatics, Center for Genetic Epidemiology and Prevention, Van Andel Research Institute, 333 Bostwick Avenue NE, Grand Rapids, MI 49503, USA.
Yan
Y
Ding
Department of Urology, Huashan Hospital, Fudan University, 12 Central Urumqi Road, Shanghai 200040, China.
Chenchen
C
Feng
Fudan-VARI Center for Genetic Epidemiology, School of Life Sciences, Fudan University, Shanghai 200433, China.
Hanming
H
Wei
Center for Cancer Genomics, Wake Forest University School of Medicine, Medical Center Boulevard, Winston-Salem, NC 27157, USA.
Sha
S
Tao
Laboratory of Computational, Laboratory of Genomics and Prevention, Center for Genetic Epidemiology and Prevention, Van Andel Research Institute, 333 Bostwick Avenue NE, Grand Rapids, MI 49503, USA.
Dan
D
Huang
Center for Cancer Genomics, Wake Forest University School of Medicine, Medical Center Boulevard, Winston-Salem, NC 27157, USA.
Siqun Lilly
SL
Zheng
Center for Cancer Genomics, Wake Forest University School of Medicine, Medical Center Boulevard, Winston-Salem, NC 27157, USA.
Jielin
J
Sun
Department of Urology, Huashan Hospital, Fudan University, 12 Central Urumqi Road, Shanghai 200040, China ; Fudan-VARI Center for Genetic Epidemiology, School of Life Sciences, Fudan University, Shanghai 200433, China ; Center for Cancer Genomics, Wake Forest University School of Medicine, Medical Center Boulevard, Winston-Salem, NC 27157, USA.
Jianfeng
J
Xu
Department of Urology, Huashan Hospital, Fudan University, 12 Central Urumqi Road, Shanghai 200040, China.
Zujun
Z
Fang
D000328:Adult; D000368:Aged; D001483:Base Sequence; D002292:Carcinoma, Renal Cell / Q000235:genetics*; D050676:Core Binding Factor Alpha 2 Subunit / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D012313:RNA / Q000235:genetics*; D000075142:RUNX1 Translocation Partner 1 Protein; D017423:Sequence Analysis, RNA; D014157:Transcription Factors / Q000235:genetics*; D059467:Transcriptome; D015854:Up-Regulation / Q000235:genetics*
D050676:Core Binding Factor Alpha 2 Subunit; D011518:Proto-Oncogene Proteins; D000075142:RUNX1 Translocation Partner 1 Protein; C493728:RUNX1 protein, human; C082222:RUNX1T1 protein, human; D014157:Transcription Factors; D012313:RNA