Acquisition of useful sero-diagnostic autoantibodies using the same patients'sera and tumor tissues.
Cancer tissues are comprised of various components including tumor cells and the surrounding tumor stroma, which consists of the extracellular matrix and inflammatory cells. Since the tumor stroma plays critical roles in tumor development, investigation of the tumor stroma in addition to tumor cells is important to identify useful tumor-associated markers. To discover novel and useful sero-diagnostic markers, a comparative study of tumor-associated autoantibodies (AAbs) in sera from lung adenocarcinoma (AC) patients was investigated by two-dimensional immunoblotting with AC cell lines or each autologous AC tissues. Autoantigens identified from tissue and cell line samples comprised 58 (45 antigens) and 53 spots (41 antigens), respectively. Thirty-six proteins including Transforming growth factor-beta-induced protein ig-h3 (BIGH3) and Hyaluronan and proteoglycan link protein 1 (HAPLN1) were detected only from tissues, 32 proteins only from cell lines, and 9 proteins from both. BIGH3 and HAPLN1 expressions were confirmed in the tumor stroma, but not in AC cell lines by immunostaining and immunoblotting. These data suggest that autologous tumor tissue and serum are important to coincidently detect AAbs derived from the tumor stroma in addition to tumor cells.
Department of Applied Tumor Pathology, Graduate School of Medical Sciences, Kitasato University, Kanagawa, Japan.
Makoto
M
Kobayashi
Ryo
R
Nagashio
Shinichiro
S
Ryuge
Yurie
Y
Murakami
Kengo
K
Yanagita
Hiroyasu
H
Nakashima
Toshihide
T
Matsumoto
Shi-Xu
SX
Jiang
Makoto
M
Saegusa
Yukitoshi
Y
Satoh
Noriyuki
N
Masuda
Yuichi
Y
Sato
D000230:Adenocarcinoma / Q000097:blood / Q000276:immunology / Q000378:metabolism; D000077192:Adenocarcinoma of Lung; D000368:Aged; D017306:Annexin A2 / Q000276:immunology / Q000378:metabolism; D000951:Antigens, Neoplasm / Q000276:immunology / Q000378:metabolism; D001323:Autoantibodies / Q000097:blood / Q000276:immunology*; D001324:Autoantigens / Q000276:immunology / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000097:blood / Q000276:immunology* / Q000378:metabolism; D045744:Cell Line, Tumor; D015180:Electrophoresis, Gel, Two-Dimensional; D016326:Extracellular Matrix Proteins / Q000276:immunology / Q000378:metabolism; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000097:blood / Q000276:immunology / Q000378:metabolism; D008297:Male; D008840:Microfilament Proteins / Q000276:immunology / Q000378:metabolism; D008875:Middle Aged; D009369:Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000276:immunology* / Q000378:metabolism; D009687:Nuclear Proteins / Q000276:immunology / Q000378:metabolism; D011509:Proteoglycans / Q000276:immunology / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000276:immunology / Q000378:metabolism; D000099249:Proteoglycan Link Protein; D000099222:betaIG-H3 Protein
D017306:Annexin A2; D000951:Antigens, Neoplasm; D001323:Autoantibodies; D001324:Autoantigens; D014408:Biomarkers, Tumor; D016326:Extracellular Matrix Proteins; D008840:Microfilament Proteins; D009687:Nuclear Proteins; D011509:Proteoglycans; D016212:Transforming Growth Factor beta; D000099249:Proteoglycan Link Protein; D000099222:betaIG-H3 Protein; C472822:ANXA2 protein, human; C066575:CAPG protein, human