Expression of monocyte chemotactic protein-1/CCL2 in gastric cancer and its relationship with tumor hypoxia.
OBJECTIVE
To investigate the expression and prognostic value of CCL2 in gastric cancer, as well as its relationship with tumor hypoxia.


METHODS
Tumor tissues from 68 gastric cancer patients (GC) were analyzed, and the expression of CCL2 and hypoxia-inducible factor 1 alpha (HIF-1α) in tumor tissues was detected by immunohistochemistry. Statistical evaluations that were used included univariate log-rank tests of Kaplan-Meier curves and multivariate Cox regression model analysis.


RESULTS
CCL2 was highly expressed in 66.2% (45/68) of gastric cancer specimens. The distribution of CCL2 expression in tumor tissue was consistent with that of HIF-1α. Patients with high CCL2 expression in GC had a lower overall survival rate [50.6 mo (95%CI: 44.44-56.93) vs 64.6 mo (95%CI: 60.27-68.94), P = 0.013].


CONCLUSIONS
CCL2 expression correlates closely with HIF-1α expression in gastric cancer. CCL2 may be an independent prognostic marker for GC.
Lei-Lei Tao, Shu-Jing Shi, Long-Bang Chen, Gui-Chun Huang, Medical Oncology Department of Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, Jiangsu Province, China.
Lei-Lei
LL
Tao
Lei-Lei Tao, Shu-Jing Shi, Long-Bang Chen, Gui-Chun Huang, Medical Oncology Department of Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, Jiangsu Province, China.
Shu-Jing
SJ
Shi
Lei-Lei Tao, Shu-Jing Shi, Long-Bang Chen, Gui-Chun Huang, Medical Oncology Department of Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, Jiangsu Province, China.
Long-Bang
LB
Chen
Lei-Lei Tao, Shu-Jing Shi, Long-Bang Chen, Gui-Chun Huang, Medical Oncology Department of Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, Jiangsu Province, China.
Gui-Chun
GC
Huang
D000328:Adult; D000368:Aged; D002277:Carcinoma / Q000378:metabolism*; D018932:Chemokine CCL2 / Q000378:metabolism*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D000860:Hypoxia; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism*; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D016016:Proportional Hazards Models; D013274:Stomach Neoplasms / Q000378:metabolism*; D016896:Treatment Outcome
C111835:CCL2 protein, human; D018932:Chemokine CCL2; C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit
Chemokines; Gastric cancer; Immunohistochemistry; Monocyte chemotactic protein-1/CCL2; Prognostic marker