Comparison of the results of PCR analysis of gene expression in breast cancer tissue specimens stabilized in formalin and RNAlater.
We compared the results of real-time PCR analysis of gene expression in paired specimens breast cancer tissue fixed in RNAlater (Qiagen) stabilization reagent (FF samples) and in formalin (FFPE samples). A clear-cut linear relationship (correlation coefficient 0.76 ± 0.07) was detected between the gene expression logarithm values in FF and FFPE samples. This fact suggests that collections of paraffin blocks with formalin-fixed tissue specimens from patients with a many-year disease history can be effectively used in modern studies.
BioClinicum Center, Moscow, Russia, khaunadia@gmail.com.
N A
NA
Khaustova
D S
DS
Makeeva
O V
OV
Kondrashina
N N
NN
Fedotov
I N
IN
Nechaev
N A
NA
Savelov
V N
VN
Grinevich
D V
DV
Maltseva
V V
VV
Galatenko
M Yu
MY
Shkurnikov
D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D018270:Carcinoma, Ductal, Breast / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D005260:Female; D005404:Fixatives / Q000737:chemistry; D005557:Formaldehyde / Q000737:chemistry; D015870:Gene Expression; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D008875:Middle Aged; D020871:RNA Stability; D011933:Reagent Kits, Diagnostic; D016707:Tissue Fixation / Q000379:methods*
D005404:Fixatives; D011933:Reagent Kits, Diagnostic; D005557:Formaldehyde