Enrichment of umbilical cord blood mononuclears with hemopoietic precursors in co-culture with mesenchymal stromal cells from human adipose tissue.
We demonstrated the possibility of enrichment of umbilical cord blood mononuclear fraction with early non-differentiated precursors under conditions of co-culturing with mesenchymal stromal cells from the human adipose tissue. It was established that umbilical cord blood mononuclear cells adhered to mesenchymal stromal cell feeder and then proliferate and differentiate into hemopoietic cells. In comparison with the initial umbilical cord blood mononuclear fraction, the cell population obtained after 7-day expansion contained 2-fold more CFU and 33.4 ± 9.5 and 24.2 ± 11.2% CD34(+) and CD133(+) cells, respectively, which corresponds to enrichment of precursor cell population by 148 ± 60. The proposed scheme of expansion of hemopoietic cells from umbilical cord blood is economically expedient and can widely used in biology and medicine.
Institute of Biomedical Problems, Russian Academy of Sciences, Moscow, Russia, evmaslova@mail.ru.
E V
EV
Maslova
E R
ER
Andreeva
I V
IV
Andrianova
P I
PI
Bobyleva
Yu A
YA
Romanov
N V
NV
Kabaeva
E E
EE
Balashova
S S
SS
Ryaskina
T N
TN
Dugina
L B
LB
Buravkova
D000273:Adipose Tissue / Q000166:cytology; D049109:Cell Proliferation; D002469:Cell Separation; D002470:Cell Survival; D002478:Cells, Cultured; D018920:Coculture Techniques; D061252:Feeder Cells / Q000502:physiology; D005260:Female; D005312:Fetal Blood / Q000166:cytology*; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000502:physiology*