Scleractinium coral aquaculture skeleton: a possible 3D scaffold for cell cultures and bone tissue engineering.
Cytocompatibility of 5 coral aquaculture skeleton species derived from two families (Acroporidae and Pocilloporidae) was studied over the course of in vitro culturing in continuous human fibroblast culture by the MMT test. Biocompatibility and capacity of scaffold to "transfer" cell cultures (specifically, multipotent mesenchymal stromal cells) to sites of implantation were studied in vivo by subcutaneous implantation of skeletal fragments to rats. All coral skeleton aquaculture specimens were cytocompatible (nontoxic and with surface matrix characteristics satisfactory for cells), biocompatible, and could be tried as 3D matrices for bone tissue engineering.
P. A. Hertsen Moscow Research Cancer Institute, Ministry of Health of the Russian Federation, Moscow, Russia.
N S
NS
Sergeeva
T A
TA
Britaev
I K
IK
Sviridova
S A
SA
Akhmedova
V A
VA
Kirsanova
A A
AA
Popov
A I
AI
Antokhin
G A
GA
Frank
A D
AD
Kaprin
D000818:Animals; D037421:Anthozoa; D001861:Bone Regeneration; D018786:Bone Substitutes / Q000633:toxicity*; D018929:Cell Culture Techniques; D002470:Cell Survival; D002478:Cells, Cultured; D005347:Fibroblasts / Q000502:physiology; D006801:Humans; D008297:Male; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000502:physiology; D019736:Prostheses and Implants; D017208:Rats, Wistar; D054457:Tissue Scaffolds
D018786:Bone Substitutes