Identification of non-coding RNAs with a new composite feature in the Hybrid Random Forest Ensemble algorithm.
To identify non-coding RNA (ncRNA) signals within genomic regions, a classification tool was developed based on a hybrid random forest (RF) with a logistic regression model to efficiently discriminate short ncRNA sequences as well as long complex ncRNA sequences. This RF-based classifier was trained on a well-balanced dataset with a discriminative set of features and achieved an accuracy, sensitivity and specificity of 92.11%, 90.7% and 93.5%, respectively. The selected feature set includes a new proposed feature, SCORE. This feature is generated based on a logistic regression function that combines five significant features-structure, sequence, modularity, structural robustness and coding potential-to enable improved characterization of long ncRNA (lncRNA) elements. The use of SCORE improved the performance of the RF-based classifier in the identification of Rfam lncRNA families. A genome-wide ncRNA classification framework was applied to a wide variety of organisms, with an emphasis on those of economic, social, public health, environmental and agricultural significance, such as various bacteria genomes, the Arthrospira (Spirulina) genome, and rice and human genomic regions. Our framework was able to identify known ncRNAs with sensitivities of greater than 90% and 77.7% for prokaryotic and eukaryotic sequences, respectively. Our classifier is available at http://ncrna-pred.com/HLRF.htm.
Biological Engineering Program, Faculty of Engineering, King Mongkut's University of Technology Thonburi, 126 Pracha Uthit Rd, Bangmod, Thung Khru, Bangkok 10140, Thailand.
Supatcha
S
Lertampaiporn
Biochemical Engineering and Pilot Plant Research and Development Unit, National Center for Genetic Engineering and Biotechnology at King Mongkut's University of Technology Thonburi (Bang Khun Thian Campus), 49 Soi Thian Thale 25, Bang Khun Thian Chai Thale Rd, Tha Kham, Bangkok 10150, Thailand.
Chinae
C
Thammarongtham
School of Information Technology, King Mongkut's University of Technology Thonburi, 126 Pracha Uthit Rd, Bangmod, Thung Khru, Bangkok 10140, Thailand.
Chakarida
C
Nukoolkit
Biological Engineering Program, Faculty of Engineering, King Mongkut's University of Technology Thonburi, 126 Pracha Uthit Rd, Bangmod, Thung Khru, Bangkok 10140, Thailand.
Boonserm
B
Kaewkamnerdpong
Biotechnology Program, School of Bioresources and Technology, King Mongkut's University of Technology Thonburi (Bang Khun Thian Campus), 49 Soi Thian Thale 25, Bang Khun Thian Chai Thale Rd, Tha Kham, Bangkok 10150, Thailand Bioinformatics and Systems Biology Program, King Mongkut's University of Technology Thonburi (Bang Khun Thian Campus), 49 Soi Thian Thale 25, Bang Khun Thian Chai Thale Rd, Tha Kham, Bangkok 10150, Thailand marasri.rue@kmutt.ac.th.
Marasri
M
Ruengjitchatchawalya
D000465:Algorithms; D002965:Classification / Q000379:methods; D016680:Genome, Bacterial; D023281:Genomics; D006801:Humans; D016015:Logistic Models; D062085:RNA, Long Noncoding / Q000235:genetics*; D058727:RNA, Small Untranslated / Q000235:genetics*; D022661:RNA, Untranslated / Q000145:classification / Q000235:genetics
D062085:RNA, Long Noncoding; D058727:RNA, Small Untranslated; D022661:RNA, Untranslated