Occurrence of aflatoxin B1 and ochratoxin A in dried vine fruits from Greek market.
Twenty-six samples of dried vine fruits from Athens and Thebes (Central Greece) market were simultaneously extracted and cleaned up by immunoaffinity columns and analysed for aflatoxin B1 (AFB1) and ochratoxin A (OTA). A combination of ELISA and HPLC methods was applied for the determination of AFB1. Recovery was 97.6%, RSD 6.46%, while the limit of detection (LOD) and limit of quantification (LOQ) were 0.05 μg kg(-1) and 0.09 μg kg(-1), respectively. OTA concentrations were only estimated by ELISA. Results revealed the presence of AFB1 in 23% of the samples (mean 1.4 μg AFB1 kg(-1)), but none exceeded the EU limit (2 μg AFB1 kg(-1)). However, OTA was detected in 100% of the samples (mean 47.2 μg OTA kg(-1)). Six samples were found to be contaminated at high levels (median 120.6 μg OTA kg(-1)) and 18 exceeded the EU limit (10 μg OTA kg(-1)).
a Department of Food Chemistry, Faculty of Chemistry , National and Kapodistrian University of Athens , Athens , Greece.
Eleni
E
Kollia
Alexandros
A
Kanapitsas
Panagiota
P
Markaki
D016604:Aflatoxin B1 / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004797:Enzyme-Linked Immunosorbent Assay; D005638:Fruit / Q000737:chemistry*; D006115:Greece; D057230:Limit of Detection; D009793:Ochratoxins / Q000032:analysis*; D013050:Spectrometry, Fluorescence
D009793:Ochratoxins; C025589:ochratoxin A; D016604:Aflatoxin B1
ELISA; HPLC; aflatoxin B1; dried vine fruits; ochratoxin A