The base discriminating potential of pyrrolidinyl PNA demonstrated by magnetic Fe(x)O(y) particles.
Pyrrolidinyl PNA was immobilized on Fe(x)O(y) magnetic particles and was able to capture and thereby discriminate single base alterations in DNA counterstrands better than DNA. The selectivities of matched vs. mismatched oligonucleotides measured by the absorption differences were up to 10-12 which are remarkable values for linear probes.
Institute of Organic Chemistry, Karlsruhe Institute of Technology (KIT), Fritz-Haber-Weg 6, 76131 Karlsruhe, Germany. Wagenknecht@kit.edu.
Claudia
C
Stubinitzky
Tirayut
T
Vilaivan
Hans-Achim
HA
Wagenknecht
D065966:Absorption, Physicochemical; D001483:Base Sequence; D019921:Biotinylation; D018076:DNA, Complementary / Q000737:chemistry; D005290:Ferric Compounds / Q000737:chemistry*; D060328:Magnetic Phenomena; D008969:Molecular Sequence Data; D009692:Nucleic Acid Heteroduplexes; D020135:Peptide Nucleic Acids / Q000737:chemistry*; D011687:Purines / Q000737:chemistry*; D011743:Pyrimidines / Q000737:chemistry*; D011759:Pyrrolidines / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D044366:Transition Temperature
D018076:DNA, Complementary; D005290:Ferric Compounds; D009692:Nucleic Acid Heteroduplexes; D020135:Peptide Nucleic Acids; D011687:Purines; D011743:Pyrimidines; D011759:Pyrrolidines; C000499:ferric oxide