Involvement of SgvP in carbon-sulfur bond formation during Griseoviridin biosynthesis.
Griseoviridin (GV) is an A-type streptogramin antibiotic displaying antimicrobial activity and acting synergistically with viridogrisein (VG). Bioinformatic analyses reveal SgvP as the sole cytochrome P450 enzyme in the GV/VG gene cluster. To explore the role of SgvP in the GV/VG pathway, we inactivated the sgvP gene. The resulting ΔsgvP mutant generated two new products: GV-1 and GV-2, both lacking the CS bridge. In trans complementation of the sgvP gene into the ΔsgvP mutant strain partially restores GV production. Feeding [1-(13) C]-labeled cysteine to the wild-type strain led to enrichment of C-7 in the GV scaffold, thus verifying that the CS bond in GV is formed through direct coupling of the free SH group provided by the side chain of cysteine. The above results highlight the significance of SgvP in CS bond formation in griseoviridin biosynthesis.
CAS Key Laboratory of Tropical Marine Bioresources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Rd., Guangzhou 510301 (China).
Yunchang
Y
Xie
Qinglian
Q
Li
Yongxiang
Y
Song
Junying
J
Ma
Jianhua
J
Ju
D002244:Carbon / Q000737:chemistry / Q000378:metabolism*; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism*; D008968:Molecular Conformation; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D013302:Streptomyces / Q000201:enzymology; D013455:Sulfur / Q000737:chemistry / Q000378:metabolism*
D010455:Peptides; C010583:griseoviridin; D013455:Sulfur; D002244:Carbon; D003577:Cytochrome P-450 Enzyme System
P450 monooxygenases; antibiotics; biosynthesis; carbon-sulfur bonds; griseoviridin; viridogrisein