Facile generation of cell microarrays using vacuum degassing and coverslip sweeping.
A simple method to generate cell microarrays with high-percentage well occupancy and well-defined cell confinement is presented. This method uses a synergistic combination of vacuum degassing and coverslip sweeping. The vacuum degassing step dislodges air bubbles from the microwells, which in turn enables the cells to enter the microwells, while the physical sweeping step using a glass coverslip removes the excess cells outside the microwells. This low-cost preparation method provides a simple solution to generating cell microarrays that can be performed in basic research laboratories and point-of-care settings for routine cell-based screening assays.
Food Science and Technology Department, University of California at Davis, Davis, CA 95616, USA.
Min S
MS
Wang
Department of Biological and Agricultural Engineering, University of California at Davis, Davis, CA 95616, USA.
Zhen
Z
Luo
Department of Biological and Agricultural Engineering, University of California at Davis, Davis, CA 95616, USA.
Sundar
S
Cherukuri
Food Science and Technology Department, University of California at Davis, Davis, CA 95616, USA; Department of Biological and Agricultural Engineering, University of California at Davis, Davis, CA 95616, USA. Electronic address: nnitin@ucdavis.edu.
Nitin
N
Nitin
D000970:Antineoplastic Agents / Q000494:pharmacology; D018929:Cell Culture Techniques / Q000295:instrumentation*; D002945:Cisplatin / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D046888:Tissue Array Analysis / Q000295:instrumentation*; D014618:Vacuum
D000970:Antineoplastic Agents; D002945:Cisplatin
Cells; Drug response; Fluorescence microscopy; Microwell array; Screening