Simultaneous determination of 15 phenolic constituents of Chinese black rice wine by HPLC-MS/MS with SPE.
This study established a new method for quantitative and qualitative determination of certain components in black rice wine, a traditional Chinese brewed wine. Specifically, we combined solid-phase extraction and high-performance liquid chromatography (HPLC) with triple quadrupole mass spectrometry (MS/MS) to determine 8 phenolic acids, 3 flavonols, and 4 anthocyanins in black rice wine. First, we clean samples with OASIS HLB cartridges and optimized extraction parameters. Next, we performed separation on a SHIM-PACK XR-ODS column (I.D. 3.0 mm × 75 mm, 2.2 μm particle size) with a gradient elution of 50% aqueous acetonitrile (V/V) and water, both containing 0.2% formic acid. We used multiple-reaction monitoring scanning for quantification, with switching electrospray ion source polarity between positive and negative modes in a single chromatographic run. We detected 15 phenolic compounds properly within 38 min under optimized conditions. Limits of detection ranged from 0.008 to 0.030 mg/L, and average recoveries ranged from 60.8 to 103.1% with relative standard deviation ≤8.6%. We validated the method and found it to be sensitive and reliable for quantifying phenolic compounds in rice wine matrices.


CONCLUSIONS
This study developed a new, reliable HPLC-MS/MS method for simultaneous determination of 15 bioactive components in black rice wine. This method was validated and found to be sensitive and reliable for quantifying phenolic compounds in rice wine.
College of Food Science and Engineering, Northwest A&F Univ, Yangling 712100, Shaanxi, P.R. China.
Yutang
Y
Wang
Yuanyuan
Y
Liu
Chunxia
C
Xiao
Laping
L
Liu
Miao
M
Hao
Jianguo
J
Wang
Xuebo
X
Liu
D000872:Anthocyanins / Q000032:analysis; D002681:China; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D044948:Flavonols / Q000032:analysis; D005504:Food Analysis / Q000379:methods*; D006801:Humans; D012275:Oryza / Q000737:chemistry*; D010636:Phenols / Q000032:analysis; D010936:Plant Extracts / Q000737:chemistry*; D059808:Polyphenols / Q000032:analysis*; D015203:Reproducibility of Results; D052616:Solid Phase Extraction / Q000379:methods; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods; D014920:Wine / Q000032:analysis*
D000872:Anthocyanins; D044948:Flavonols; D010636:Phenols; D010936:Plant Extracts; D059808:Polyphenols
HPLC-MS/MS; anthocyanin; black rice wine; phenolic constituents; solid-phase extraction