Tobacco experimental model to induce urinary bladder neoplasms.
OBJECTIVE
to develop an experimental model of exposure to tobacco burning (cigarette) products to assess the effects of its chronic use in relation to cancers of the bladder.


METHODS
the animals were chronically exposed to the burning tobacco products in a semi-open chamber to simulate smoking. Thirty young Wistar rats were divided into two groups: one with 20 animals simulating smoking for six months, and ten not exposed control animals for the same period. After exposure by inhalation of cigarette smoke, animals were euthanized and subjected to histopathological study of the bladder wall.


RESULTS
no tumor was found but mild and non significant alterations. The studies of hemo-oximetry (carboxyhemoglobin and methemoglobin) and the concentration of carbon dioxide (CO2) confirm that the animals were exposed to high concentrations of tobacco smoke and its derivatives.


CONCLUSIONS
no bladder mucosal neoplasia was found in the pathological study of animals. The developed experimental models were highly efficient, practical and easy to use and can be used in other similar studies to determine the harmful effects caused by smoking.
School of Medicine, UNICAMP.
José Alexandre
JA
Colli Neto
School of Medicine, UNICAMP.
José Hélio
JH
Zen Júnior
Department of Surgery, UNICAMP.
André
A
Del Negro
Department of Surgery, UNICAMP.
Nelson Adami
NA
Andreollo
Center for Experimental Medicine and Surgery, UNICAMP.
Marina Rachel
MR
Araujo
Department of Surgery, UNICAMP.
Alfio José
AJ
Tincani
D000818:Animals; D004195:Disease Models, Animal; D008297:Male; D009374:Neoplasms, Experimental / Q000209:etiology; D051381:Rats; D017208:Rats, Wistar; D062789:Tobacco Products; D001749:Urinary Bladder Neoplasms / Q000209:etiology*