Mitochondrial changes in platelets are not related to those in skeletal muscle during human septic shock.
Platelets can serve as general markers of mitochondrial (dys)function during several human diseases. Whether this holds true even during sepsis is unknown. Using spectrophotometry, we measured mitochondrial respiratory chain biochemistry in platelets and triceps brachii muscle of thirty patients with septic shock (within 24 hours from admission to Intensive Care) and ten surgical controls (during surgery). Results were expressed relative to citrate synthase (CS) activity, a marker of mitochondrial density. Patients with septic shock had lower nicotinamide adenine dinucleotide dehydrogenase (NADH)/CS (p = 0.015), complex I/CS (p = 0.018), complex I and III/CS (p<0.001) and complex IV/CS (p = 0.012) activities in platelets but higher complex I/CS activity (p = 0.021) in triceps brachii muscle than controls. Overall, NADH/CS (r2 = 0.00; p = 0.683) complex I/CS (r(2) = 0.05; p = 0.173), complex I and III/CS (r(2) = 0.01; p = 0.485), succinate dehydrogenase (SDH)/CS (r(2) = 0.00; p = 0.884), complex II and III/CS (r(2) = 0.00; p = 0.927) and complex IV/CS (r(2) = 0.00; p = 0.906) activities in platelets were not associated with those in triceps brachii muscle. In conclusion, several respiratory chain enzymes were variably inhibited in platelets, but not in triceps brachii muscle, of patients with septic shock. Sepsis-induced mitochondrial changes in platelets do not reflect those in other organs.
U.O. Terapia Intensiva "Emma Vecla", Fondazione IRCCS Ca' Granda - Ospedale Maggiore Policlinico, Università degli Studi di Milano, Milan, Italy.
Alessandro
A
Protti
U.O. Neurologia - Centro Dino Ferrari - Fondazione IRCCS Ca' Granda - Ospedale Maggiore Policlinico, Università degli Studi di Milano, Milan, Italy.
Francesco
F
Fortunato
U.O. Terapia Intensiva "Emma Vecla", Fondazione IRCCS Ca' Granda - Ospedale Maggiore Policlinico, Università degli Studi di Milano, Milan, Italy.
Maria L
ML
Caspani
U.O. Neurochirurgia, Fondazione IRCCS Ca' Granda - Ospedale Maggiore Policlinico, Università degli Studi di Milano, Milan, Italy.
Mauro
M
Pluderi
U.O. Malattie Neuromuscolari - Centro Dino Ferrari - Fondazione IRCCS Ca' Granda - Ospedale Maggiore Policlinico, Università degli Studi di Milano, Milan, Italy.
Valeria
V
Lucchini
U.O. Neurochirurgia, Fondazione IRCCS Ca' Granda - Ospedale Maggiore Policlinico, Università degli Studi di Milano, Milan, Italy.
Nadia
N
Grimoldi
U.O. Traumatologia, Fondazione IRCCS Ca' Granda - Ospedale Maggiore Policlinico, Università degli Studi di Milano, Milan, Italy.
Luigi P
LP
Solimeno
U.O. Malattie Neuromuscolari - Centro Dino Ferrari - Fondazione IRCCS Ca' Granda - Ospedale Maggiore Policlinico, Università degli Studi di Milano, Milan, Italy.
Gigliola
G
Fagiolari
U.O. Malattie Neuromuscolari - Centro Dino Ferrari - Fondazione IRCCS Ca' Granda - Ospedale Maggiore Policlinico, Università degli Studi di Milano, Milan, Italy.
Patrizia
P
Ciscato
U.O. Neurochirurgia, Fondazione IRCCS Ca' Granda - Ospedale Maggiore Policlinico, Università degli Studi di Milano, Milan, Italy.
Samis M A
SM
Zella
U.O. Malattie Neuromuscolari - Centro Dino Ferrari - Fondazione IRCCS Ca' Granda - Ospedale Maggiore Policlinico, Università degli Studi di Milano, Milan, Italy.
Maurizio
M
Moggio
U.O. Neurologia - Centro Dino Ferrari - Fondazione IRCCS Ca' Granda - Ospedale Maggiore Policlinico, Università degli Studi di Milano, Milan, Italy.
Giacomo P
GP
Comi
U.O. Terapia Intensiva "Emma Vecla", Fondazione IRCCS Ca' Granda - Ospedale Maggiore Policlinico, Università degli Studi di Milano, Milan, Italy.
Luciano
L
Gattinoni
D000328:Adult; D000368:Aged; D001792:Blood Platelets / Q000166:cytology / Q000201:enzymology*; D019069:Cell Respiration; D045222:Electron Transport Chain Complex Proteins / Q000378:metabolism*; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D008297:Male; D008875:Middle Aged; D008928:Mitochondria / Q000201:enzymology*; D018482:Muscle, Skeletal / Q000201:enzymology* / Q000473:pathology; D012772:Shock, Septic / Q000378:metabolism* / Q000473:pathology; D013053:Spectrophotometry
D045222:Electron Transport Chain Complex Proteins