Effects of low intensity laser in in vitro bacterial culture and in vivo infected wounds.
OBJECTIVE
to compare the effects of low intensity laser therapy on in vitro bacterial growth and in vivo in infected wounds, and to analyze the effectiveness of the AsGa Laser technology in in vivo wound infections.


METHODS
in vitro: Staphylococcus aureus were incubated on blood agar plates, half of them being irradiated with 904 nm wavelength laser and dose of 3J/cm² daily for seven days. In vivo: 32 male Wistar rats were divided into control group (uninfected) and Experimental Group (Infected). Half of the animals had their wounds irradiated.


RESULTS
in vitro: there was no statistically significant variation between the experimental groups as for the source plates and the derived ones (p>0.05). In vivo: there was a significant increase in the deposition of type I and III collagen in the wounds of the infected and irradiated animals when assessed on the fourth day of the experiment (p=0.034).


CONCLUSIONS
low-intensity Laser Therapy applied with a wavelength of 904 nm and dose 3J/cm² did not alter the in vitro growth of S. aureus in experimental groups; in vivo, however, it showed significant increase in the deposition of type I and III collagen in the wound of infected and irradiated animals on the fourth day of the experiment.
Pontifical Catholic University of Paraná, Curitiba, PR, Brazil.
Pâmela Rosa
PR
Pereira
Department of Surgery, Pontifical Catholic University of Paraná, Curitiba, PR, Brazil.
Josue Bruginski
JB
de Paula
Department of Pharmacy, Pontifical Catholic University of Paraná, Curitiba, PR, Brazil.
Juliette
J
Cielinski
Department of Pharmacy, Pontifical Catholic University of Paraná, Curitiba, PR, Brazil.
Marcelo
M
Pilonetto
Department of Surgery, Pontifical Catholic University of Paraná, Curitiba, PR, Brazil.
Luiz Carlos
LC
Von Bahten
D000818:Animals; D001431:Bacteriological Techniques; D054023:Lasers, Semiconductor / Q000627:therapeutic use*; D028022:Low-Level Light Therapy; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013211:Staphylococcus aureus / Q000528:radiation effects*; D014946:Wound Infection / Q000532:radiotherapy*