Homologous yeast lipases/acyltransferases exhibit remarkable cold-active properties.
Lipases/acyltransferases catalyse acyltransfer to various nucleophiles preferentially to hydrolysis even in aqueous media with high thermodynamic activity of water (a w >0.9). Characterization of hydrolysis and acyltransfer activities in a large range of temperature (5 to 80 °C) of secreted recombinant homologous lipases of the Pseudozyma antarctica lipase A superfamily (CaLA) expressed in Pichia pastoris, enlighten the exceptional cold-activity of two remarkable lipases/acyltransferases: CpLIP2 from Candida parapsilosis and CtroL4 from Candida tropicalis. The activation energy of the reactions catalysed by CpLIP2 and CtroL4 was 18-23 kJ mol(-1) for hydrolysis and less than 15 kJ mol(-1) for transesterification between 5 and 35 °C, while it was respectively 43 and 47 kJ mol(-1) with the thermostable CaLA. A remarkable consequence is the high rate of the reactions catalysed by CpLIP2 and CtroL4 at very low temperatures, with CpLIP2 displaying at 5 °C 65 % of its alcoholysis activity and 45 % of its hydrolysis activity at 30 °C. These results suggest that, within the CaLA superfamily and its homologous subgroups, common structural determinants might allow both acyltransfer and cold-active properties. Such biocatalysts are of great interest for the efficient synthesis or functionalization of temperature-sensitive lipid derivatives, or more generally to lessen the environmental impact of biocatalytic processes.
Département de génie chimique et de génie biotechnologique, Faculté de génie, Sherbrooke University, 2500, Boulevard de l'Université, Sherbrooke, Québec, J1K 2R1, Canada.
Pisey M
PM
Neang
Maeva
M
Subileau
Véronique
V
Perrier
Eric
E
Dubreucq
D000217:Acyltransferases / Q000378:metabolism*; D002175:Candida / Q000201:enzymology*; D006868:Hydrolysis; D008049:Lipase / Q000378:metabolism*; D010802:Phylogeny; D011994:Recombinant Proteins / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013696:Temperature; D014587:Ustilaginales / Q000201:enzymology
D011994:Recombinant Proteins; D000217:Acyltransferases; D008049:Lipase