Detachment and flow cytometric quantification of seagrass-associated bacteria.
A new protocol was developed to detach bacteria from seagrass tissue and subsequently enumerate cells using flow cytometry (FCM). A method involving addition of the surfactant Tween 80 and vortexing resulted in maximum detachment efficiency of seagrass attached bacteria, providing a robust protocol for precisely enumerating seagrass-associated bacteria with FCM. Using this approach we detected cell concentrations between 2.0×10(5) and 8.0×10(6)cells mg(-1) DW tissue.
Plant Functional Biology and Climate Change Cluster, University of Technology, Sydney, Australia.
Stacey
S
Trevathan-Tackett
Plant Functional Biology and Climate Change Cluster, University of Technology, Sydney, Australia.
Peter
P
Macreadie
Plant Functional Biology and Climate Change Cluster, University of Technology, Sydney, Australia. Electronic address: peter.ralph@uts.edu.au.
Peter
P
Ralph
Plant Functional Biology and Climate Change Cluster, University of Technology, Sydney, Australia.
Justin
J
Seymour
D001419:Bacteria / Q000302:isolation & purification*; D058491:Bacterial Load / Q000379:methods*; D003902:Detergents / Q000494:pharmacology; D005434:Flow Cytometry / Q000379:methods*; D011136:Polysorbates / Q000494:pharmacology; D013314:Stress, Mechanical; D035962:Zosteraceae / Q000382:microbiology*
D003902:Detergents; D011136:Polysorbates
Bacterial detachment; Enumeration; Flow cytometry; Microbe; Seagrass; Seagrass-associated bacteria