Flow injection analysis of thiamazole based on strong Ru(bpy)3 (2+) co-reactant electrochemiluminescence.
Based on the strong electrochemiluminescence (ECL) reaction between thiamazole and tris(2,2'-bipyridine)ruthenium(II) (Ru(bpy)3 (2+) ), a sensitive, simple and rapid flow injection analysis method for the determination of thiamazole was developed. When a Pt working electrode was maintained at a potential of +1.50 V (vs Ag/AgCl) in pH 12.0 H3 PO4 -NaOH solution containing thiamazole and Ru(bpy)3 (2+) at a flow rate of 1.0 mL/min, a linear range of 2.0 × 10(-7) -1.0 × 10(-4)  mol/L with a detection limit of 5.0 × 10(-8)  mol/L was obtained for the detection of thiamazole. The method showed good reproducibility with a relative standard deviation (RSD) of 0.75%. The method has been successfully applied to the determination of thiamazole in spiked animal feeds. In addition, a co-reactant ECL mechanism was proposed for the thiamazole-Ru(bpy)3 (2+) system.
School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China; MOE Key Laboratory of Analysis and Detection Technology for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, and Department of Chemistry, Fuzhou University, Fujian, 350108, China.
Dexian
D
Kong
Qinglu
Q
Li
Jiang
J
Jiang
Zheng
Z
Xinyu
Zhou
Z
Xuechou
Yuwu
Y
Chi
Guonan
G
Chen
D015082:2,2'-Dipyridyl / Q000031:analogs & derivatives* / Q000737:chemistry; D000818:Animals; D002645:Chickens; D056831:Coordination Complexes; D055664:Electrochemical Techniques; D017022:Flow Injection Analysis; D049449:Luminescence; D008163:Luminescent Measurements; D008713:Methimazole / Q000008:administration & dosage / Q000032:analysis*; D013552:Swine; D017975:Ruthenium Compounds
D015082:2,2'-Dipyridyl; D056831:Coordination Complexes; D008713:Methimazole; C547232:tris(2,2-bipyridine)-ruthenium(II); D017975:Ruthenium Compounds
antithyroid; electrochemiluminescence; flow injection; thiamazole