Immunohistochemical analysis of expressions of RB1, CDK4, HSP90, cPLA2G4A, and CHMP2B is helpful in distinction between myxofibrosarcoma and myxoid liposarcoma.
The role and diagnostic efficacy of gene and protein products RB1, CDK4, CHMP2B, HSP90, and cPLA2G4A, all previously shown to be involved in tumor genesis and cell proliferation, were examined by immunohistochemical techniques in 32 cases of myxofibrosarcomas and 29 myxoid liposarcomas (all diagnosis had been confirmed by fluorescence in situ hybridization). HSP90 demonstrated strong nuclear and cytoplasmic positivity in all myxoid liposarcoma cases, while only 4 myxofibrosarcomas showed scattered HSP90 positivity. All but 4 cases of myxofibrosarcoma displayed strong positivity for cPLA2G4A, while only 2 myxoid liposarcoma cases were cPLA2G4A positive and both were CHMP2B negative. Overexpression of both cPLA2G4A and CHMP2B also suggested higher tumor grade. In conclusion, HSP90 and cPLA2G4A immunohistochemical stains are useful markers to distinguish myxofibrosarcoma from myxoid liposarcoma.
University of Pittsburgh Medical Center-Shadyside Campus, Pittsburgh, PA, USA taowangtw@gmail.com.
Tao
T
Wang
University of Pittsburgh Medical Center-Shadyside Campus, Pittsburgh, PA, USA.
Mark A
MA
Goodman
University of Pittsburgh Medical Center-Shadyside Campus, Pittsburgh, PA, USA.
Richard L
RL
McGough
University of Pittsburgh Medical Center-Shadyside Campus, Pittsburgh, PA, USA.
Kurt R
KR
Weiss
University of Pittsburgh Medical Center-Shadyside Campus, Pittsburgh, PA, USA.
Uma N M
UN
Rao
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000032:analysis*; D051358:Cyclin-Dependent Kinase 4 / Q000032:analysis / Q000096:biosynthesis; D003937:Diagnosis, Differential; D056827:Endosomal Sorting Complexes Required for Transport / Q000032:analysis / Q000096:biosynthesis; D005260:Female; D005354:Fibrosarcoma / Q000175:diagnosis*; D054513:Group IV Phospholipases A2 / Q000032:analysis / Q000096:biosynthesis; D018841:HSP90 Heat-Shock Proteins / Q000032:analysis / Q000096:biosynthesis; D006801:Humans; D007150:Immunohistochemistry; D018208:Liposarcoma, Myxoid / Q000175:diagnosis*; D008297:Male; D008875:Middle Aged; D016160:Retinoblastoma Protein / Q000032:analysis / Q000096:biosynthesis; D055815:Young Adult
D014408:Biomarkers, Tumor; C503812:CHMP2B protein, human; D056827:Endosomal Sorting Complexes Required for Transport; D018841:HSP90 Heat-Shock Proteins; D016160:Retinoblastoma Protein; C495900:CDK4 protein, human; D051358:Cyclin-Dependent Kinase 4; C472331:PLA2G4C protein, human; D054513:Group IV Phospholipases A2
CDK4; CHMP2B; CPLA2G4A; HSP90; RB1; myxofibrosarcoma; myxoid liposarcoma