Effect of Tinospora cordifolia on the reduction of ultraviolet radiation-induced cytotoxicity and DNA damage in PC12 cells.
The safety of Tinospora cordifolia and its potential to protect against ultraviolet radiation-induced cytotoxicity and DNA damage in PC12 cells were investigated. To evaluate the safety of T. cordifolia, cell viability and agarose gel electrophoresis were carried out using PC12 cells treated with 0 to 100 μg mL(-1) of methanol extract of T. cordifolia. T. cordifolia extracts did not show cytotoxicity ranging 0 to 100 μg mL(-1). In addition, T. cordifolia extracts significantly increased cell viability at 1 ng, 10 ng and 1 μg mL(-1) concentrations in serum-deprived medium compared to control. To confirm the protective role against UV-induced damage, PC12 cells alone or in the presence of 10 ng, 100 ng, or 1 μg mL(-1) of T. cordifolia extract were exposed to 250, 270 and 290 nm of UV radiation, which corresponded to doses of 120, 150 and 300 mJ cm(-2), respectively. Treatment with T. cordifolia extracts significantly increased the cell survival rate irradiated at 290 nm. In addition, T. cordifolia extracts significantly reduced cyclobutane pyrimidine dimer formation induced by UV irradiation at all wavelengths. In conclusion, T. cordifolia is not toxic and safe for cells. Our findings can support its application as phototherapy in the medical sector.
a Course of Environmental Adaptation Science, Division of Environmental Science Development, Graduate School of Environmental Science , Hokkaido University , Sapporo , Japan.
Runa
R
Masuma
Tsutomu
T
Okuno
Mohammad Shahabuddin
MS
Kabir Choudhuri
Takeshi
T
Saito
Masaaki
M
Kurasaki
D000818:Animals; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D004249:DNA Damage / Q000187:drug effects* / Q000528:radiation effects; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical; D004587:Electrophoresis, Agar Gel; D004797:Enzyme-Linked Immunosorbent Assay; D016716:PC12 Cells / Q000187:drug effects / Q000528:radiation effects; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry; D011740:Pyrimidine Dimers / Q000378:metabolism; D011837:Radiation-Protective Agents / Q000494:pharmacology; D051381:Rats; D031610:Tinospora / Q000737:chemistry*; D014466:Ultraviolet Rays / Q000009:adverse effects*
D010936:Plant Extracts; D011740:Pyrimidine Dimers; D011837:Radiation-Protective Agents
Cell viability; DNA damage; UV radiation; cyclobutane pyrimidine dimer