Episodic ozone exposure in adult and senescent Brown Norway rats: acute and delayed effect on heart rate, core temperature and motor activity.
Setting exposure standards for environmental pollutants may consider the aged as a susceptible population but the few published studies assessing susceptibility of the aged to air pollutants are inconsistent. Episodic ozone (O₃) is more reflective of potential exposures occurring in human populations and could be more harmful to the aged. This study used radiotelemetry to monitor heart rate (HR), core temperature (T(c)) and motor activity (MA) in adult (9-12 months) and senescent (20-24 months) male, Brown Norway rats exposed to episodic O₃ (6 h/day of 1 ppm O₃ for 2 consecutive days/week for 13 weeks). Acute O₃ initially led to marked drops in HR and T(c). As exposures progressed each week, there was diminution in the hypothermic and bradycardic effects of O₃. Senescent rats were less affected than adults. Acute responses were exacerbated on the second day of O₃ exposure with adults exhibiting greater sensitivity. During recovery following 2 d of O₃, adult and senescent rats exhibited an elevated T(c) and HR during the day but not at night, an effect that persisted for at least 48 h after O₃ exposure. MA was elevated in adults but not senescent rats during recovery from O₃. Overall, acute effects of O₃, including reductions in HR and T(c), were attenuated in senescent rats. Autonomic responses during recovery, included an elevation in T(c) with a pattern akin to that of a fever and rise in HR that were independent of age. An attenuated inflammatory response to O₃ in senescent rats may explain the relatively heightened physiological response to O₃ in younger rats.
Toxicity Assessment Division, U.S. Environmental Protection Agency, Research Triangle Park, NC, USA .
C J
CJ
Gordon
A F
AF
Johnstone
C
C
Aydin
P M
PM
Phillips
R C
RC
MacPhail
U P
UP
Kodavanti
A D
AD
Ledbetter
K A
KA
Jarema
D000375:Aging; D000393:Air Pollutants / Q000633:toxicity*; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D001833:Body Temperature Regulation / Q000187:drug effects; D001919:Bradycardia / Q000139:chemically induced*; D006339:Heart Rate / Q000187:drug effects; D007035:Hypothermia / Q000139:chemically induced*; D019570:Inhalation Exposure / Q000009:adverse effects*; D008297:Male; D009043:Motor Activity / Q000187:drug effects; D020258:Neurotoxicity Syndromes / Q000503:physiopathology*; D010083:Oxidants, Photochemical / Q000633:toxicity; D010126:Ozone / Q000633:toxicity*; D011914:Rats, Inbred BN; D012720:Severity of Illness Index; D013618:Tachyphylaxis; D023382:Toxicity Tests, Acute; D059550:Toxicity Tests, Subchronic; D066007:Toxicokinetics
D000393:Air Pollutants; D010083:Oxidants, Photochemical; D010126:Ozone
Aging; air pollution; fever; hypothermia