A red-shifted fluorescent substrate for aldehyde dehydrogenase.
Selection of cells positive for aldehyde dehydrogenase (ALDH) activity from a green-fluorescent background is difficult with existing reagents. Here we report a red-shifted fluorescent substrate for ALDH, AldeRed 588-A, for labelling viable ALDH(pos) cells. We demonstrate that AldeRed 588-A successfully isolates ALDH(hi) human haematopoietic stem cells from heterogeneous cord blood mononuclear cells. AldeRed 588-A can be used for multicolour applications to fractionate ALDH(pos) cells in the presence of green fluorophores including the ALDEFLUOR reagent and cells expressing enhanced green-fluorescent protein (eGFP). AldeRed 588-A stains ALDH(pos) murine pancreatic centroacinar and terminal duct cells, as visualized using fluorescent microscopy. AldeRed588-A provides a useful tool to select stem cells or study ALDH within a green-fluorescent background.
1] Russel H. Morgan Department of Radiology and Radiological Science, Baltimore, Maryland 21287, USA [2].
Il
I
Minn
1] Russel H. Morgan Department of Radiology and Radiological Science, Baltimore, Maryland 21287, USA [2].
Haofan
H
Wang
Russel H. Morgan Department of Radiology and Radiological Science, Baltimore, Maryland 21287, USA.
Ronnie C
RC
Mease
College of Pharmacy, Korea University, 2511 Sejong-ro, Jochiwon-eup, Sejong 339-700, South Korea.
Youngjoo
Y
Byun
Russel H. Morgan Department of Radiology and Radiological Science, Baltimore, Maryland 21287, USA.
Xing
X
Yang
Department of Surgery and the McKusick-Nathans Institute of Genetic Medicine, Baltimore, Maryland 21205, USA.
Julia
J
Wang
Department of Surgery and the McKusick-Nathans Institute of Genetic Medicine, Baltimore, Maryland 21205, USA.
Steven D
SD
Leach
1] Russel H. Morgan Department of Radiology and Radiological Science, Baltimore, Maryland 21287, USA [2] Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, Baltimore, Maryland 21287, USA.
Martin G
MG
Pomper
D000444:Aldehyde Dehydrogenase / Q000378:metabolism*; D000818:Animals; D045744:Cell Line, Tumor; D004396:Coloring Agents / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000378:metabolism*; D006412:Hematopoietic Stem Cells / Q000378:metabolism; D006801:Humans; D007202:Indicators and Reagents; D051379:Mice
D004396:Coloring Agents; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D000444:Aldehyde Dehydrogenase