Simultaneous determination of seven constituents in Si-Ni-San decoction and a compatibility comparison study using HPLC-UV.
A simple and accurate HPLC-UV method was developed for simultaneous determination of seven constituents in Si-Ni-San decoction. Separation was performed on a Hypersil C18 column and detection was set with gradient wavelength at 240 nm (0-26 min) and 210 nm (26-30 min). The mobile phase consisted of 0.03% phosphoric acid in water (v/v), and acetonitrile was used with a flow rate of 1.0 (- 1). This method provides good linearity (r>0.9992), precision (RSD < 1.9%), repeatability (RSD < 2.9%), stability (RSD < 2.9%) and recovery (97.6-102.0%), which has been successfully applied to quantitative determination of the seven constituents in Si-Ni-San decoction and different compatibility groups.
a School of Pharmacy, Shenyang Pharmaceutical University , 110016 Shenyang , China.
Jing
J
Wen
Ying
Y
Qiao
Zhili
Z
Xiong
Famei
F
Li
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry*; D007202:Indicators and Reagents; D015394:Molecular Structure; D029596:Paeonia; D013056:Spectrophotometry, Ultraviolet
D004365:Drugs, Chinese Herbal; D007202:Indicators and Reagents; C510945:shigyaku-san
HPLC–UV; Si-Ni-San; compatibility; simultaneous determination