Preparation and characterization of polylactide/poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) hybrid fibers for potential application in bone tissue engineering.
The aim of this study was to develop a kind of osteogenic biodegradable composite graft consisting of human placenta-derived mesenchymal stem cell (hPMSC) material for site-specific repair of bone defects and attenuation of clinical symptoms. The novel nano- to micro-structured biodegradable hybrid fibers were prepared by electrospinning. The characteristics of the hybrid membranes were investigated by a range of methods, including Fourier transform infrared spectroscopy, X-ray diffraction, and differential scanning calorimetry. Morphological study with scanning electron microscopy showed that the average fiber diameter and the number of nanoscale pores on each individual fiber surface decreased with increasing concentration of poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) (PCEC). The prepared polylactide (PLA)/PCEC fibrous membranes favored hPMSC attachment and proliferation by providing an interconnected, porous, three-dimensional mimicked extracellular environment. What is more, hPMSCs cultured on the electrospun hybrid PLA/PCEC fibrous scaffolds could be effectively differentiated into bone-associated cells by positive alizarin red staining. Given the good cellular response and excellent osteogenic potential in vitro, the electrospun PLA/PCEC fibrous scaffolds could be one of the most promising candidates for bone tissue engineering.
State Key Laboratory of Biotherapy and Cancer Center, Chengdu, People's Republic of China ; Department of Neurosurgery, West China Hospital, West China Medical School, Sichuan University, Chengdu, People's Republic of China.
YueLong
Y
Wang
State Key Laboratory of Biotherapy and Cancer Center, Chengdu, People's Republic of China.
Gang
G
Guo
Department of Neurosurgery, West China Hospital, West China Medical School, Sichuan University, Chengdu, People's Republic of China.
HaiFeng
H
Chen
State Key Laboratory of Biotherapy and Cancer Center, Chengdu, People's Republic of China.
Xiang
X
Gao
State Key Laboratory of Biotherapy and Cancer Center, Chengdu, People's Republic of China.
RangRang
R
Fan
State Key Laboratory of Biotherapy and Cancer Center, Chengdu, People's Republic of China.
DongMei
D
Zhang
Department of Neurosurgery, West China Hospital, West China Medical School, Sichuan University, Chengdu, People's Republic of China.
LiangXue
L
Zhou
D018786:Bone Substitutes / Q000138:chemical synthesis*; D016025:Bone Transplantation / Q000295:instrumentation; D002478:Cells, Cultured; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005240:Feasibility Studies; D005260:Female; D006801:Humans; D007773:Lactates / Q000737:chemistry*; D008422:Materials Testing; D045164:Mesenchymal Stem Cell Transplantation / Q000295:instrumentation* / Q000379:methods; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology; D010012:Osteogenesis / Q000502:physiology*; D011091:Polyesters / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011247:Pregnancy; D023822:Tissue Engineering / Q000295:instrumentation; D054457:Tissue Scaffolds
D018786:Bone Substitutes; D007773:Lactates; D011091:Polyesters; C088513:poly(lactic acid-ethylene glycol); D011092:Polyethylene Glycols; C062968:lactide-caprolactone copolymer
PCEC; PLA; bone tissue engineering; electrospinning; hPMSCs