Physiological, biochemical and histological alterations induced by administration of imidacloprid in female albino rats.
Imidacloprid, a neonicotinoid the newest class of major insecticide has outstanding potency and systemic action for crop protection against piercing and sucking insects pests and also highly effective for control of flea on cats and dogs. The effect of oral administration of two doses of imidacloprid 10 and 20mg/kg/day for 60 days on biochemical parameters, histopathology and protein profile of female albino rat was assessed. Average feed intake was significantly reduced (P<0.01) at 20mg/kg/day. Relative weight of heart and spleen decreased significantly (P<0.05) at higher dose level. Non significant increase in alanine aminotransferase (ALT), aspartate aminotransferase (AST), acid phosphatase (ACP), alkaline phosphatase (AKP) activity was observed in both the imidacloprid treated groups. There was significant decrease (P<0.01, P<0.05) in acetyl cholinesterase (AChE) activity in plasma and brain of both the imidacloprid treated groups. Microscopically, liver tissue of rats treated with higher dose of imidacloprid showed marked dilation and congestion of central vein and degeneration of hepatocytes. The exposure to imidacloprid produced histopathological changes that could be correlated with changes in the biochemical profile of female albino rats. The blood plasma proteins were examined by SDS PAGE. There was no diagnostic difference in the pattern of plasma protein profile of control and treated rats. Based on the present physiological, biochemical and histological studies it is evident that imidacloprid did not produce any significant effects at 10mg/kg/day dose but induced toxicological effects at 20mg/kg/day to female rats.
Department of Zoology, College of Basic Sciences and Humanities, Punjab Agricultural University, Ludhiana 141004, Punjab, India. Electronic address: vohra.prerna123@gmail.com.
Prerna
P
Vohra
Department of Zoology, College of Basic Sciences and Humanities, Punjab Agricultural University, Ludhiana 141004, Punjab, India.
Kuldeep Singh
KS
Khera
Department of Zoology, College of Basic Sciences and Humanities, Punjab Agricultural University, Ludhiana 141004, Punjab, India.
Gurinder Kaur
GK
Sangha
D000110:Acetylcholinesterase / Q000097:blood / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000201:enzymology; D004435:Eating / Q000187:drug effects; D030762:Estrous Cycle / Q000187:drug effects; D005260:Female; D006321:Heart / Q000187:drug effects; D007093:Imidazoles / Q000633:toxicity*; D007306:Insecticides / Q000633:toxicity*; D008099:Liver / Q000187:drug effects / Q000473:pathology; D000073943:Neonicotinoids; D009574:Nitro Compounds / Q000633:toxicity*; D019055:No-Observed-Adverse-Effect Level; D009929:Organ Size / Q000187:drug effects; D017208:Rats, Wistar; D013154:Spleen / Q000187:drug effects / Q000473:pathology
D007093:Imidazoles; D007306:Insecticides; D000073943:Neonicotinoids; D009574:Nitro Compounds; C082359:imidacloprid; D000110:Acetylcholinesterase
ALT; AST; Acetyl cholinesterase; Female albino rats; Histopathology; Imidacloprid