Sweet pepper confirmed as a reservoir host for tomato yellow leaf curl virus by both agro-inoculation and whitefly-mediated inoculation.
Tomato yellow leaf curl virus (TYLCV), a member of the genus Begomovirus, has a single-stranded DNA genome. TYLCV can induce severe disease symptoms on tomato plants, but other hosts plants such as cucurbits and peppers are asymptomatic. A full-length DNA clone of a Korean TYLCV isolate was constructed by rolling-circle amplification from TYLCV-infected tomatoes in Korea. To assess relative susceptibility of sweet pepper varieties to TYLCV, 19 cultivars were inoculated with cloned TYLCV by agro-inoculation. All TYLCV-infected sweet peppers were asymptomatic, even though Southern hybridization and polymerase chain reaction analysis showed TYLCV genomic DNA accumulation in roots, stems, and newly produced shoots. Southern hybridization indicated that TYLCV replicated and moved systemically from agro-inoculated apical shoot tips to roots or newly produced shoots of sweet peppers. Whitefly-mediated inoculation experiments showed that TYLCV can be transmitted to tomatoes from TYLCV-infected sweet peppers. Taken together, these results indicate that sweet pepper can be a reservoir for TYLCV in nature.
Department of Genetic Engineering, Sungkyunkwan University, 300 Cheoncheon-dong, Jangan-gu, Suwon, 440-746, Republic of Korea.
Eui-Joon
EJ
Kil
Hee-Seong
HS
Byun
Sunhoo
S
Kim
Jaedeok
J
Kim
Jungan
J
Park
Seungchan
S
Cho
Dong-Cheol
DC
Yang
Kyeong-Yeoll
KY
Lee
Hong-Soo
HS
Choi
Ji-Kwang
JK
Kim
Sukchan
S
Lee
D000818:Animals; D053839:Begomovirus / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification; D015139:Blotting, Southern; D002212:Capsicum / Q000821:virology*; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D006430:Hemiptera / Q000821:virology; D007303:Insect Vectors; D007723:Korea; D018551:Solanum lycopersicum / Q000821:virology; D008969:Molecular Sequence Data; D018517:Plant Roots / Q000821:virology; D018520:Plant Shoots / Q000821:virology; D018547:Plant Stems / Q000821:virology; D017422:Sequence Analysis, DNA
D004279:DNA, Viral