BTEB2 prevents neuronal apoptosis via promoting bad phosphorylation in rat intracerebral hemorrhage model.
Krüppel-like zinc-finger transcription factor 5 (KLF5), known as BTEB2 or IKLF, has several biological functions that involve cell proliferation, development and apoptosis. Previous studies demonstrated that BTEB2 had anti-apoptotic effect in multiple diseases such as esophageal cancer and non-small cell lung cancers (NSCLCs). However, the distribution and function of BTEB2 in CNS diseases remain unknown. In this study, we show that BTEB2 down-regulates neuronal apoptosis during pathophysiological processes of intracerebral hemorrhage (ICH). A rat ICH model was established by behavioral tests. Western blot and immunohistochemistry revealed a remarkable up-regulation of BTEB2 expression surrounding the hematoma after ICH. Double-labeled immunofluorescence showed BTEB2 was mostly co-localized with neurons, rarely with activated astrocytes and microglia. Furthermore, we detected that neuronal apoptosis marker active caspase-3 had co-localizations with BTEB2. In addition, KLF5 knockdown in vitro specifically resulted in increasing neuronal apoptosis coupled with reduced Bad phosphorylation at both ser112 and ser136 residues. All our findings suggested that BTEB2 down-regulated neuronal apoptosis via promoting Bad phosphorylation after ICH.
Key Laboratory of Glycoconjugate Research, Ministry of Health, Department of Biochemistry and Molecular Biology, Shanghai Medical College, Fudan University, Shanghai, 200032, China.|Department of Pathogen Biology, Medical College, Nantong University, Nantong, Jiangsu, 226001, China.
Xiaojuan
X
Liu
Department of Pathogen Biology, Medical College, Nantong University, Nantong, Jiangsu, 226001, China.
Damin
D
Yuan
Department of Nutrition and Food Hygiene, School of Public Health, Nantong University, Nantong, Jiangsu, 226001, China.
Xiaoke
X
Nie
Department of Neurology, Affiliated Hospital of Nantong, Nantong University, Nantong, Jiangsu, 226001, China.
Jianhong
J
Shen
Department of Neurology, Affiliated Hospital of Nantong, Nantong University, Nantong, Jiangsu, 226001, China.
Yaohua
Y
Yan
Department of Pathogen Biology, Medical College, Nantong University, Nantong, Jiangsu, 226001, China. m15162784780@163.com.
Dongmei
D
Zhang
Key Laboratory of Glycoconjugate Research, Ministry of Health, Department of Biochemistry and Molecular Biology, Shanghai Medical College, Fudan University, Shanghai, 200032, China.|Medical College, Nantong University, Nantong, Jiangsu, 226001, China.
Jianxin
J
Gu
D000818:Animals; D017209:Apoptosis; D002543:Cerebral Hemorrhage / Q000378:metabolism*; D051741:Kruppel-Like Transcription Factors / Q000235:genetics / Q000378:metabolism*; D008297:Male; D009474:Neurons / Q000378:metabolism*; D016716:PC12 Cells; D010766:Phosphorylation; D051381:Rats; D017207:Rats, Sprague-Dawley; D051025:bcl-Associated Death Protein / Q000378:metabolism*
C492215:Klf5 protein, rat; D051741:Kruppel-Like Transcription Factors; D051025:bcl-Associated Death Protein