Human epidermal keratinocyte cell response on integrin-specific artificial extracellular matrix proteins.
Cell-matrix interactions play critical roles in regulating cellular behavior in wound repair and regeneration of the human skin. In particular, human skin keratinocytes express several key integrins such as alpha5beta1, alpha3beta1, and alpha2beta1 for binding to the extracellular matrix (ECM) present in the basement membrane in uninjured skin. To mimic these key integrin-ECM interactions, artificial ECM (aECM) proteins containing functional domains derived from laminin 5, type IV collagen, fibronectin, and elastin are prepared. Human skin keratinocyte cell responses on the aECM proteins are specific to the cell-binding domain present in each construct. Keratinocyte attachment to the aECM protein substrates is also mediated by specific integrin-material interactions. In addition, the aECM proteins are able to support the proliferation of keratinocyte stem cells, demonstrating their promise for use in skin tissue engineering.
School of Materials Science and Engineering, Nanyang Technological University, Block N4.1, Nanyang Avenue, 639798, Singapore.
Monica Suryana
MS
Tjin
Alvin Wen Choong
AW
Chua
Dong Rui
DR
Ma
Seng Teik
ST
Lee
Eileen
E
Fong
D015153:Blotting, Western; D001973:Bromodeoxyuridine; D015815:Cell Adhesion Molecules / Q000737:chemistry; D060326:Chemistry Techniques, Synthetic; D024141:Collagen Type IV / Q000737:chemistry; D003114:Colony-Forming Units Assay; D017931:DNA Primers / Q000235:genetics; D004549:Elastin / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D000078404:Epidermal Cells; D016326:Extracellular Matrix Proteins / Q000737:chemistry* / Q000378:metabolism; D005353:Fibronectins / Q000737:chemistry; D005455:Fluorescent Antibody Technique; D006801:Humans; D016023:Integrins / Q000378:metabolism*; D015603:Keratinocytes / Q000187:drug effects* / Q000378:metabolism; D011994:Recombinant Proteins / Q000138:chemical synthesis* / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000097794:Kalinin
D001973:Bromodeoxyuridine; D015815:Cell Adhesion Molecules; D024141:Collagen Type IV; D017931:DNA Primers; D004549:Elastin; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D016023:Integrins; D011994:Recombinant Proteins; D000097794:Kalinin
artificial ECM proteins; elastin-like polypeptides; keratinocytes; skin; tissue engineering