Comparison of a novel TiO₂/diatomite composite and pure TiO₂ for the purification of phosvitin phosphopeptides.
A novel TiO2/diatomite composite (TD) was prepared and then characterized by scanning electron microscope (SEM) and Fourier Transform Infrared (FTIR). The results of SEM showed that after modification, the porous surface of diatomite was covered with TiO2. Both diatomite and TD had clear disc-shaped structures with average grain diameters of around 25 μm. Then TD and pure TiO2 were applied in the purification of phosvitin phosphopeptides (PPPs) from the digest of egg yolk protein, and a comparative study of adsorption properties of PPPs on TD and TiO2 was performed. In the study of adsorption kinetics, the adsorption equilibrium of PPPs on TD and TiO2 fitted well with the Langmuir model, and the time needed to reach adsorption equilibrium were both around 10 min. The maximum dynamic adsorption capacity of TD (8.15 mg/g) was higher than that of TiO2 (4.96 mg/g). The results of repeated use showed that TD and TiO2 were very stable after being subjected to ten repeated adsorption-elution cycles, and TD could easily be separated from aqueous solution by filtration. On the other hand, the present synthetic technology of TD was very simple, cost-effective, organic solvent-free and available for large-scale preparation. Thus, this separation method not only brings great advantages in the purification of PPPs from egg yolk protein but also provides a promising purification material for the enrichment of phosphopeptides in proteomic researches.
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, PR China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, PR China.
Yang
Y
Zhang
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, PR China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, PR China.
Junhua
J
Li
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, PR China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, PR China.
Fuge
F
Niu
School of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013, PR China.
Jun
J
Sun
Shenzhen Entry-Exit Inspection and Quarantine Bureau, Shenzhen, Guangdong 518045, PR China.
Yuan
Y
Dou
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, PR China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, PR China.
Yuntao
Y
Liu
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, PR China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, PR China.
Yujie
Y
Su
Kang de Biologicals Co., Ltd. Nantong.
Bei
B
Zhou
Kang de Biologicals Co., Ltd. Nantong.
Qinqin
Q
Xu
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, PR China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, PR China. Electronic address: yiershasha91zy@126.com.
Yanjun
Y
Yang
D000327:Adsorption; D007692:Diatomaceous Earth / Q000737:chemistry*; D004355:Drug Stability; D004530:Egg Yolk / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D010748:Phosphopeptides / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D010774:Phosvitin / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D014025:Titanium / Q000737:chemistry*
D010748:Phosphopeptides; C009495:titanium dioxide; D007692:Diatomaceous Earth; C033787:diatomite; D010774:Phosvitin; D014025:Titanium
Phosvitin phosphopeptides; Purification; TiO(2); TiO(2)/diatomite composite; Yolk