Experimental applications of TNF-reporter mice with far-red fluorescent label.
This chapter provides protocols for in vitro and in vivo analysis of TNF-producing cells from a novel TNF reporter mouse. In these transgenic mice, genetic sequence encoding far-red reporter protein Katyushka (FRFPK) was placed under control of the same regulatory elements as TNF, thus providing the basis for detection, isolation, and visualization of TNF-producing cells.
German Rheumatism Research Center, a Leibniz Institute, Berlin, Germany.
Yury V
YV
Shebzukhov
Anna A
AA
Kuchmiy
Andrei A
AA
Kruglov
Frauke
F
Zipp
Volker
V
Siffrin
Sergei A
SA
Nedospasov
D000818:Animals; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000378:metabolism / Q000473:pathology*; D005434:Flow Cytometry; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism*; D007091:Image Processing, Computer-Assisted; D007202:Indicators and Reagents / Q000378:metabolism; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D011401:Promoter Regions, Genetic / Q000235:genetics; D011995:Recombination, Genetic; D018800:Thy-1 Antigens / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology*; D000097573:Red Fluorescent Protein
D049452:Green Fluorescent Proteins; D007202:Indicators and Reagents; D008164:Luminescent Proteins; D018800:Thy-1 Antigens; D014409:Tumor Necrosis Factor-alpha; D000097573:Red Fluorescent Protein; C485184:enhanced green fluorescent protein