Engineering the stereochemistry of cephalosporin for specific detection of pathogenic carbapenemase-expressing bacteria.
Reported herein is the design of fluorogenic probes specific for carbapenem-resistant Enterobacteriaceae (CRE) and they were designed based on stereochemically modified cephalosporin having a 6,7-trans configuration. Through experiments using recombinant β-lactamase enzymes and live bacterial species, these probes demonstrate the potential for use in the specific detection of carbapenemases, including metallo-β-lactamases in active bacterial pathogens.
Departments of Radiology and Chemistry, Molecular Imaging Program at Stanford, Stanford University School of Medicine, Stanford, CA 94305 (USA).
Haibin
H
Shi
Yunfeng
Y
Cheng
Kyung Hyun
KH
Lee
Robert F
RF
Luo
Niaz
N
Banaei
Jianghong
J
Rao
D001419:Bacteria / Q000187:drug effects* / Q000201:enzymology; D001426:Bacterial Proteins / Q000378:metabolism*; D002511:Cephalosporins / Q000737:chemistry / Q000494:pharmacology*; D013237:Stereoisomerism; D001618:beta-Lactamases / Q000378:metabolism*
D001426:Bacterial Proteins; D002511:Cephalosporins; D001618:beta-Lactamases; C063912:carbapenemase
enzymes; fluorescence; fluorescent probes; metalloenzymes; stereochemistry