Molecular prevalence and genetic diversity of bovine Theileria orientalis in Myanmar.
Theileria orientalis is a causative agent of benign theileriosis in cattle and distributed in mainly Asian countries. In the present study, we examined the prevalence of T. orientalis infection by PCR based on the major piroplasm surface protein gene (MPSP) sequences in cattle in Myanmar, followed by phylogenetic analysis of the MPSP genes. The MPSP gene was amplified in 258 of 713 (36.2%) cattle blood DNA samples collected from five cities in different geographical regions of Myanmar. Phylogenetic analysis of MPSP sequences from 54 T. orientalis-positive DNA samples revealed the presence of six allelic genotypes, including Types 1, 3, 4, 5, 7, and N-3. Types 5 and 7 were the predominant types detected. Sequences of the MPSP genes detected in Myanmar were closely related to those from Thailand, Vietnam or Mongolia. These findings suggest that movement of animals carrying T. orientalis parasites between Southeast Asian countries could be a reason for the similar genotype distribution of the parasites in Myanmar.
Laboratory of Parasitology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan; Department of Pharmacology and Parasitology, University of Veterinary Science, Nay Pyi Taw 05282, Myanmar.
Saw
S
Bawm
Laboratory of Parasitology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
Kohei
K
Shimizu
Laboratory of Parasitology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
Jun-Ichi
J
Hirota
Laboratory of Parasitology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
Yusuke
Y
Tosa
Department of Pharmacology and Parasitology, University of Veterinary Science, Nay Pyi Taw 05282, Myanmar.
Lat Lat
LL
Htun
Livestock Breeding and Veterinary Department (Head Quarter), Nay Pyi Taw, Myanmar.
Ni Ni
NN
Maw
Livestock Breeding and Veterinary Department (Head Quarter), Nay Pyi Taw, Myanmar.
Myint
M
Thein
Laboratory of Parasitology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
Hirotomo
H
Kato
Laboratory of Parasitology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
Tatsuya
T
Sakurai
Laboratory of Parasitology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan. Electronic address: kenkata@vetmed.hokudai.ac.jp.
Ken
K
Katakura
D000483:Alleles; D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics* / Q000378:metabolism; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology* / Q000469:parasitology*; D014644:Genetic Variation; D005838:Genotype; D008969:Molecular Sequence Data; D002052:Myanmar / Q000453:epidemiology; D010802:Phylogeny; D015995:Prevalence; D015800:Protozoan Proteins / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA / Q000662:veterinary; D012689:Sequence Homology, Nucleic Acid; D016794:Theileria / Q000235:genetics* / Q000302:isolation & purification; D013801:Theileriasis / Q000453:epidemiology* / Q000469:parasitology*
D000953:Antigens, Protozoan; D015800:Protozoan Proteins; C115173:major piroplasm surface protein, Theileria
Cattle; Genetic diversity; MPSP gene; Myanmar; Theileria orientalis