Anti-Listeria starters: in vitro selection and production plant evaluation.
Anti-Listeria bacterial starters are highly demanded by the meat industry. Novel bioprotective anti-Listeria starters were searched among Lactobacillus species strains isolated from artisanal sausages. The screening confirmed that anti-Listeria activity is a strain-specific property and yielded only 1 strain (of 36) exhibiting a satisfactory level of inhibition, L. delbrueckii WC0286. This strain was compared with two commercial bioprotective starters, SafePro B-SF-43 and SafePro B-LC-20, in a model simulating in vitro the first step of the fermentation process. The presence of the bioprotective starters did not modify the pH in such a way that could affect the safety or organoleptic properties of the product. Both SafePro B-SF-43 and SafePro B-LC-20 effected an important reduction of Listeria counts (0.56 and 0.72 log CFU g(-1), respectively, in 72 h), while the anti-Listeria effect of L. delbrueckii WC0286 was minor (0.15 log CFU g(-1)). These results discouraged the utilization of L. delbrueckii WC0286 for a challenge test in a pilot salami production, in favor of the best-performing bioprotective starter, SafePro B-LC-20. The test confirmed that SafePro B-LC-20 did not alter the acidification trend of sausages and was capable of inhibiting Listeria, which decreased by 1.21 log CFU g(-1). This information is relevant to address research activity toward the development of new bioprotective starters. The data herein presented demonstrate that the efficacy in Listeria control of potentially bioprotective bacterial starters requires further validation in real meat matrixes, possibly by using in vitro meat fermentation experiments to narrow down the list of candidates before pilot scale challenge tests.
Dipartimento di Scienze della Vita, Università di Modena e Reggio Emilia, via G. Campi 183, 41125 Modena, Italy. stefano.raimondi@unimore.it.
Stefano
S
Raimondi
Dipartimento di Scienze della Vita, Università di Modena e Reggio Emilia, via G. Campi 183, 41125 Modena, Italy.
Mina
M
Popovic
Dipartimento di Scienze della Vita, Università di Modena e Reggio Emilia, via G. Campi 183, 41125 Modena, Italy.
Alberto
A
Amaretti
Dipartimento di Scienze Agrarie, Università di Bologna, Viale Fanin 44, 40127 Bologna, Italy.
Diana
D
Di Gioia
Dipartimento di Scienze della Vita, Università di Modena e Reggio Emilia, via G. Campi 183, 41125 Modena, Italy.
Maddalena
M
Rossi
D000818:Animals; D000898:Antibiosis; D005285:Fermentation; D005516:Food Microbiology; D007778:Lactobacillus / Q000502:physiology*; D008087:Listeria / Q000254:growth & development*; D008461:Meat Products / Q000382:microbiology*; D013045:Species Specificity; D013552:Swine