Intracanal delivery of Resolvin E1 controls inflammation in necrotic immature rat teeth.
BACKGROUND
Pulp necrosis in immature teeth and the resulting periodontal apical inflammation negatively affect root formation. Resolvin E1 (RvE1) is a lipid-derived endogenous pro-resolution molecule that controls inflammation. The aim of this investigation was to evaluate the impact of RvE1 applied as an intracanal medication on root formation in nonvital immature teeth.


METHODS
To arrest root development, pulpectomy was performed in the lower first molars of 4-week-old Wistar rats. After 3 weeks, irrigation with 2.5% sodium hypochlorite and 0.9% sterile saline was performed, and either a triple antibiotic paste (TAP) or RvE1 in saline was applied into the root canals. In the control group, access openings drilled into molars were left exposed to the oral environment. Root development and periapical repair were evaluated radiographically and histologically at 3 and 6 weeks after treatment.


RESULTS
RvE1 reduced periapical lesion size compared with the control at 3 weeks, which was similar to TAP. Inflammatory response in the RvE1-treated group was markedly reduced compared with both TAP and control specimens. At 6 weeks, root development was observed in both groups, but RvE1 treatment produced less cellularity with more regular calcified tissue deposition.


CONCLUSIONS
RvE1 and TAP had a positive impact on reducing inflammation and promoting root formation. RvE1 was more effective in reducing inflammation at earlier stages. RvE1 has potential to be used as root canal dressing to control inflammation in endodontically compromised teeth before complete root formation. Stability of RvE1 within the root canal and its delivery are issues to be addressed before its clinical use.
Pontificia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, Rio Grande do Sul, Brazil.
Roberta Kochenborger
RK
Scarparo
Pontificia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, Rio Grande do Sul, Brazil.
Lenara
L
Dondoni
Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brazil.
Daiana Elisabeth
DE
Böttcher
Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brazil.
Fabiana Soares
FS
Grecca
Pontificia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, Rio Grande do Sul, Brazil.
Jose Antonio P
JA
Figueiredo
Department of Periodontology and Translational Biology Research, Forsyth Institute, Cambridge, Massachusetts.
Alpdogan
A
Kantarci
Department of Periodontology and Translational Biology Research, Forsyth Institute, Cambridge, Massachusetts.
Thomas E
TE
Van Dyke
Division of Periodontology, Department of Diagnostics and Surgical Sciences, Faculty of Dentistry, University of Manitoba, Winnipeg, Manitoba, Canada; Department of Oral Biology, Faculty of Dentistry, University of Manitoba, Winnipeg, Manitoba, Canada. Electronic address: eraldo.batistajunior@umanitoba.ca.
Eraldo Luiz
EL
Batista
D000818:Animals; D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D002939:Ciprofloxacin / Q000627:therapeutic use; D003786:Dental Pulp Cavity / Q000187:drug effects*; D003790:Dental Pulp Necrosis / Q000188:drug therapy*; D015118:Eicosapentaenoic Acid / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000627:therapeutic use; D008297:Male; D008795:Metronidazole / Q000627:therapeutic use; D008911:Minocycline / Q000627:therapeutic use; D009805:Odontogenesis / Q000187:drug effects; D010485:Periapical Periodontitis / Q000628:therapy; D011670:Pulpectomy / Q000379:methods; D051381:Rats; D017208:Rats, Wistar; D012388:Root Canal Irrigants / Q000627:therapeutic use*; D012973:Sodium Hypochlorite / Q000627:therapeutic use; D014074:Tooth Calcification / Q000187:drug effects; D014092:Tooth Root / Q000187:drug effects* / Q000254:growth & development; D019553:Tooth, Nonvital / Q000188:drug therapy*
D000900:Anti-Bacterial Agents; D000893:Anti-Inflammatory Agents; D012388:Root Canal Irrigants; D008795:Metronidazole; D002939:Ciprofloxacin; D015118:Eicosapentaenoic Acid; D012973:Sodium Hypochlorite; D008911:Minocycline; C499823:5S,12R,18R-trihydroxy-6Z,8E,10E,14Z,16E-eicosapentaenoic acid
Immature teeth; Resolvin E1; inflammation resolution; root development