Effect of factors produced by the placenta on cytokine secretion by THP-1 cells cultured on a 3D scaffold.
We compared the effects of soluble products from the placenta obtained from women with normal pregnancy and pregnancy complicated by preeclampsia on cytokine secretion by THP-1 cells cultured on a 3D Matrigel scaffold. In the presence of soluble products from all placentas, the cells actively secreted IL-8, MCP-1, and soluble forms of CD14, TNFRI, and TNFRII receptors. Secretion of VEGF was below the spontaneous level. Secretion of IL-6 by THP-1 cells after incubation with soluble products of the placentas obtained during weeks 9-11 of physiological pregnancy and 38-39 of pregnancy complicated by preeclampsia surpassed the spontaneous level. In the presence of soluble factors of trimester I placentas, secretion of IL-6 and soluble form of TNFRI receptor was higher than in the presence of trimester III placental factors. Secretion of IL-6 by THP-1 cells was higher, while secretion of soluble TNFRII receptor was lower in the presence of placentas from women with preeclampsia in comparison with physiological pregnancy.
D. O. Ott Research Institute of Obstetrics and Gynecology, North-Western Division of the Russian Academy of Medical Sciences, St. Petersburg, Russia.
Ya S
YS
Onokhina
T Yu
TY
L'vova
D Z
DZ
Tsitskarava
D A
DA
Koren'kov
S A
SA
Sel'kov
D I
DI
Sokolov
D018929:Cell Culture Techniques; D002460:Cell Line; D016207:Cytokines / Q000378:metabolism*; D005260:Female; D006801:Humans; D010920:Placenta / Q000502:physiology*; D011225:Pre-Eclampsia / Q000276:immunology / Q000378:metabolism; D011247:Pregnancy; D014020:Tissue Extracts / Q000502:physiology
D016207:Cytokines; D014020:Tissue Extracts