A polysaccharide from pomegranate peels induces the apoptosis of human osteosarcoma cells via the mitochondrial apoptotic pathway.
Pomegranate (Punica granatum L.) peels, as one of the most valuable by-products of the food industry, has attracted much attention due to its wide range of bioactivities. In this study, the potential anticancer effect of a polysaccharide from pomegranate peels (PPP) on human osteosarcoma cancer cells was investigated. PPP was found to induce the arrest of G2/M phase, induce apoptosis, and inhibit the growth of U-2 osteosarcoma (OS) cells in a dose-dependent manner. Moreover, Western blotting analysis showed that PPP triggered the mitochondrial apoptotic pathway, as indicated by an increase in Bax/Bcl-2 ratios, a loss of mitochondrial membrane potential, release of cytochrome c, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells. Our results indicate that PPP inhibits the proliferation of human osteosarcoma cancer cells by inducing apoptosis through the intrinsic mitochondrial pathway.
Department of Orthopaedic Surgery, Tang Du Hospital, The Fourth Military Medical University, Xi'an, 710038, China.
Jun
J
Li
Fujun
F
Zhang
Shaohua
S
Wang
D017209:Apoptosis / Q000187:drug effects*; D020169:Caspases / Q000502:physiology; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006801:Humans; D029561:Lythraceae / Q000737:chemistry*; D008928:Mitochondria / Q000187:drug effects* / Q000502:physiology; D012516:Osteosarcoma / Q000188:drug therapy* / Q000473:pathology; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis; D051028:bcl-2-Associated X Protein / Q000032:analysis
C494826:BAX protein, human; D011134:Polysaccharides; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D020169:Caspases