Selective and universal primers for trematode barcoding in freshwater snails.
Trematodes are significant pathogens of high medical, veterinary, and environmental importance. They are hard to isolate from their intermediate hosts, and their early life stages are difficult to identify morphologically. Therefore, primers were developed for trematodes to create a species barcoding system and allow selective PCR amplification in mixed samples. The specific oligonucleotide primer was universal for trematodes that infected several freshwater snail species in Israel. The diagnostic tool is based on the 18S rDNA gene. In contrast to morphological identification, trematode barcoding is rapid as it is based on a sequence of only 800 bp, and it classifies species accurately due to high polymorphism between conserved areas.
Department of Zoology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, 6997801, Israel.
J
J
Routtu
D
D
Grunberg
R
R
Izhar
Y
Y
Dagan
Y
Y
Guttel
M
M
Ucko
F
F
Ben-Ami
D000818:Animals; D058893:DNA Barcoding, Taxonomic / Q000379:methods; D017931:DNA Primers / Q000235:genetics*; D005618:Fresh Water; D020459:Genes, rRNA; D007557:Israel; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D018510:RNA, Helminth / Q000235:genetics*; D012337:RNA, Ribosomal, 18S / Q000187:drug effects / Q000235:genetics*; D012908:Snails / Q000469:parasitology*; D014200:Trematoda / Q000145:classification* / Q000235:genetics
D017931:DNA Primers; D018510:RNA, Helminth; D012337:RNA, Ribosomal, 18S