Chemical compositions by using LC-MS/MS and GC-MS and biological activities of Sedum sediforme (Jacq.) Pau.
In this research, the chemical composition and biological activities of various extracts obtained from whole parts of Sedum sediforme (Jacq.) Pau were compared. The amounts of total phenolic and flavonoid components in crude extracts were determined by expression as pyrocatechol and quercetin equivalents, respectively. All of the extracts (petroleum ether, acetone, methanol, and water) obtained from S. sediforme showed strong antioxidant activity in four tested methods. Particularly, the IC50 values of the methanol extract, which was the richest in terms of total phenolic and flavonoid contents, were found to be lower than those of α-tocopherol and BHT in β-carotene bleaching (9.78 ± 0.06 μg/mL), DPPH free radical scavenging (9.07 ± 0.07 μg/mL), and ABTS cation radical scavenging (5.87 ± 0.03 μg/mL) methods. Furthermore, the methanol extract of S. sediforme showed higher inhibition activity than galanthamine against acetyl- and butyryl-cholinesterase enzymes. Also, acetone and methanol extracts exhibited moderate antimicrobial activity against Candida albicans. The main constituents of fatty acid and essential oil were identified as palmitic acid (C16:0) (28.8%) and α-selinene (20.4%), respectively, by GC-MS. In the methanol extract of S. sediforme, quercetin, rutin, naringenin, and protocatechuic, p-coumaric, caffeic, and chlorogenic acids were detected and quantified by LC-MS/MS. Results of the current study showed that the methanol extract of S. sediforme may also be used as a food supplement.
Department of Pharmacognosy, Faculty of Pharmacy, Dicle University , 21280 Diyarbakır, Turkey.
Abdulselam
A
Ertaş
Mehmet
M
Boğa
Mustafa Abdullah
MA
Yılmaz
Yeter
Y
Yeşil
Nesrin
N
Haşimi
Meryem Şeyda
MŞ
Kaya
Hamdi
H
Temel
Ufuk
U
Kolak
D000935:Antifungal Agents / Q000737:chemistry / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry; D002176:Candida albicans / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005227:Fatty Acids / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D009822:Oils, Volatile / Q000737:chemistry / Q000494:pharmacology; D010636:Phenols / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology; D031963:Sedum / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods
D000935:Antifungal Agents; D000975:Antioxidants; D005227:Fatty Acids; D009822:Oils, Volatile; D010636:Phenols; D010936:Plant Extracts