[Detection, diagnosis and analysis of the first case of neonatal alloimmune thrombocytopenia purpura associated with anti-HPA-5b in China].
This study was aimed to investigate the detection and diagnosis of the neonatal alloimmune thrombocytopenia purpura (NAITP) caused by anti-HPA-5b antibody. The platelet count and clinical manifestation in the newborn were examined. The HPA-1-21bw genotypes of the newborn and her parents were detected by multiple-PCR and DNA sequencing. The HPA-specific antibody in the sera of newborn and her mother were detected and identified by flow cytometry (FCM) and monoclonal antibody-specific immobilization of platelet antigens (MAIPA). The results indicated that the clinical manifestations of the newborn were lighter. The HPA genotyping showed that the genotype of the newborn was HPA-5ab, while that of her mother and father were HPA-5aa and HPA-5ab, respectively. The antibody against the platelet of newborn's father existed in the newborn's mother sera. The HPA antibody of the mother was identified as anti-HPA-5b. It is concluded that the newborn with neonatal alloimmune thrombocytopenia purpura was caused by the antibody against HPA-5b.
Nanning Institute of Transfusion Medicine, Nanning Blood Center, Nanning 530007, Guangxi Zhuang Autonomous Region, China.
Yan
Y
Zhou
Nanning Institute of Transfusion Medicine, Nanning Blood Center, Nanning 530007, Guangxi Zhuang Autonomous Region, China.
Zhou-Lin
ZL
Zhong
Nanning Institute of Transfusion Medicine, Nanning Blood Center, Nanning 530007, Guangxi Zhuang Autonomous Region, China.
Li-Lan
LL
Li
Nanning Institute of Transfusion Medicine, Nanning Blood Center, Nanning 530007, Guangxi Zhuang Autonomous Region, China.
Wei-Dong
WD
Shen
Nanning Institute of Transfusion Medicine, Nanning Blood Center, Nanning 530007, Guangxi Zhuang Autonomous Region, China. E-mail: guangwu@szonline.net.
Guo-Guang
GG
Wu
D016824:Antigens, Human Platelet / Q000235:genetics*; D002681:China; D005260:Female; D005838:Genotype; D006801:Humans; D007231:Infant, Newborn; D016553:Purpura, Thrombocytopenic, Idiopathic / Q000175:diagnosis* / Q000235:genetics; D054098:Thrombocytopenia, Neonatal Alloimmune / Q000175:diagnosis* / Q000235:genetics
C055973:5b alloantigen, human; D016824:Antigens, Human Platelet