[Study on the optimum combinations of plant hormone in callus culture of Thermopsis lanceolata].
OBJECTIVE
To explore the effects of different hormone combinations on callus induction of Thermopsis lanceolata.


METHODS
The effects of different hormone concentrations and ratio on callus induction and subculture multiplication of Thermopsis lanceolata were investigated by orthogonal design methods.


RESULTS
The optimal medium of callus induction for hypocotyls explants was MS + 2, 4-D (0.5 mg/L) + BA (1.0 mg/L) + NAA (1.0 mg/L) + IAA (0.5 mg/L). The best medium of callus subculture multiplication for hypocotyl explants was MS with 2,4-D (0.1 mg/L) + BA (0.1 mg/L) + NAA (0.2 mg/L) + IAA (0.5 mg/L) . In the process of callus subculture multiplication, the effect extent of four kinds of hormones for hypocotyl explants was BA > 2,4-D > IAA > NAA. The growth curve of callus culture optimal hormone combination was "S" curve.


CONCLUSIONS
The rapid callus culture system for Thermopsis lanceolata is established for the first time. It provides a new way for protecting and utilizing Thermopsis lanceolata species resources through the modern biotechnology.
Xiu-Fang
XF
Zheng
Xiao-Dong
XD
Wang
Chao-Qiang
CQ
Zhang
En-Jun
EJ
Wang
D015084:2,4-Dichlorophenoxyacetic Acid / Q000494:pharmacology; D000704:Analysis of Variance; D003470:Culture Media / Q000737:chemistry; D007887:Fabaceae / Q000187:drug effects* / Q000254:growth & development*; D018546:Hypocotyl / Q000187:drug effects / Q000254:growth & development; D010937:Plant Growth Regulators / Q000737:chemistry / Q000494:pharmacology*; D012639:Seeds / Q000187:drug effects / Q000254:growth & development; D046509:Tissue Culture Techniques / Q000379:methods*
D003470:Culture Media; D010937:Plant Growth Regulators; D015084:2,4-Dichlorophenoxyacetic Acid