CARI ONE induces anagen phase of telogenic hair follicles through regulation of β-catenin, stimulation of dermal papilla cell proliferation, and melanogenesis.
The use of herbal mixtures in the hair growth market has increased dramatically over the last decade. In this study, we investigated the hair growth-promoting activity of CARI ONE, a mixture of medicinal plants and mushrooms, in telogenic 6-week-old C57BL/6N mice. CARI ONE promoted hair growth through stimulation of the telogen to anagen transition. Histomorphometry analysis data indicated that topical application of CARI ONE induced an earlier anagen phase and prolonged the mature anagen phase, and also increased the number and size of hair follicles (HFs) as compared to either the control or 1% minoxidil-treated group. Immunohistochemical analysis revealed an earlier induction of β-catenin and Trp-1 protein in the HFs of the CARI ONE-treated group compared to that in the control group or 1% minoxidil-treated group. In vitro, CARI ONE promoted the proliferation of dermal papilla cells and resulted in increased melanin content in B16F10 cells in a dose-dependent manner without affecting cell viability. These results suggest that CARI ONE promotes hair growth through induction of the anagen phase in resting HFs.
Department of Food Science and Biotechnology, College of BioNano Technology, Gachon University , Sungnam 461-701 , South Korea.
Hye-Jin
HJ
Park
D000287:Administration, Topical; D000818:Animals; D001688:Biological Products / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D020405:Dermis / Q000166:cytology / Q000187:drug effects*; D005658:Fungi; D006197:Hair / Q000187:drug effects* / Q000254:growth & development; D018859:Hair Follicle / Q000187:drug effects* / Q000378:metabolism; D007370:Interferon Type I / Q000378:metabolism; D019684:Magnoliopsida; D008543:Melanins / Q000378:metabolism*; D008810:Mice, Inbred C57BL; D010946:Plants, Medicinal; D011257:Pregnancy Proteins / Q000378:metabolism; D050052:TRPC Cation Channels / Q000378:metabolism; D051176:beta Catenin / Q000378:metabolism*
D001688:Biological Products; D007370:Interferon Type I; D008543:Melanins; D011257:Pregnancy Proteins; D050052:TRPC Cation Channels; D051176:beta Catenin; C020947:interferon tau; C096351:transient receptor potential cation channel, subfamily C, member 1
CARI ONE; anagen; dermal papilla cells (DPCs); melanogenesis; β-catenin