Automatic method for focusing biological specimens in digital lensless holographic microscopy.
A self-focusing method applicable to digital lensless holographic microscopy is presented. The method searches for the global minimum of the area enclosing a given amount of energy in a region surrounding the object of interest. The proposed modified enclosed energy method has been tested on self-focusing experimental holograms of a paramecium specimen and a section of the head of a drosophila melanogaster fly. The presented self-focusing technique also has been contrasted with some of the already reported methods to seek the best focus image.
Carlos A
CA
Trujillo
Jorge
J
Garcia-Sucerquia
D000465:Algorithms; D000818:Animals; D001185:Artificial Intelligence; D004331:Drosophila melanogaster; D006257:Head / Q000033:anatomy & histology*; D006696:Holography / Q000379:methods*; D007089:Image Enhancement / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D007909:Lenses; D018613:Microscopy, Confocal / Q000295:instrumentation / Q000379:methods*; D010363:Pattern Recognition, Automated / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012815:Signal Processing, Computer-Assisted