In cell scalaradial interactome profiling using a bio-orthogonal clickable probe.
A bio-orthogonal click-chemistry procedure was developed to allow the in cell interactome profiling of scalaradial, an anti-inflammatory marine natural product. The results were validated through the application of the classical in vitro chemical proteomics and several bio-physical methods; peroxiredoxins, 14-3-3 isoforms and proteasomes were recognized as main scalaradial targets.
Department of Pharmacy, University of Salerno, Via Giovanni Paolo II 132, 84084, Fisciano, Salerno, Italy. mcmonti@unisa.it casapullo@unisa.it.
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Cassiano
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Margarucci
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Esposito
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Riccio
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Tosco
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Casapullo
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Monti
D048948:14-3-3 Proteins / Q000032:analysis / Q000378:metabolism*; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001386:Azides / Q000737:chemistry; D057930:Click Chemistry / Q000379:methods*; D006367:HeLa Cells; D006718:Homosteroids / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D054464:Peroxiredoxins / Q000032:analysis / Q000378:metabolism*; D054830:Sesterterpenes / Q000737:chemistry / Q000494:pharmacology*; D053719:Tandem Mass Spectrometry
D048948:14-3-3 Proteins; D000893:Anti-Inflammatory Agents; D001386:Azides; D006718:Homosteroids; D054830:Sesterterpenes; C071197:scalaradial; D054464:Peroxiredoxins