CAZyme discovery and design for sweet dreams.
Development of synthetic routes to complex carbohydrates and glyco-conjugates is often hampered by the lack of enzymes with requisite properties or specificities. Indeed, assembly or degradation of carbohydrates requires carbohydrate-active enzymes (CAZymes) able to act on a vast range of glycosidic monomers, oligomers or polymers in a regio-specific or stereo-specific manner in order to produce the desired structure. Sequence-based analyses allow finding the most original enzymes. Novel screening methods have emerged that enable a more efficient exploitation of the CAZyme diversity found in the microbial world or generated by protein engineering. Computational biology methods also play a prominent role in the success of CAZyme design. Such progress allows circumventing current limitations of carbohydrate synthesis and opens new opportunities related to the synthetic biology field.
Université de Toulouse; INSA,UPS,INP; LISBP, 135 Avenue de Rangueil, F-31077 Toulouse, France; CNRS, UMR5504, F-31400 Toulouse, France; INRA, UMR792 Ingénierie des Systèmes Biologiques et des Procédés, F-31400 Toulouse, France.
Isabelle
I
André
Université de Toulouse; INSA,UPS,INP; LISBP, 135 Avenue de Rangueil, F-31077 Toulouse, France; CNRS, UMR5504, F-31400 Toulouse, France; INRA, UMR792 Ingénierie des Systèmes Biologiques et des Procédés, F-31400 Toulouse, France.
Gabrielle
G
Potocki-Véronèse
Université de Toulouse; INSA,UPS,INP; LISBP, 135 Avenue de Rangueil, F-31077 Toulouse, France; CNRS, UMR5504, F-31400 Toulouse, France; INRA, UMR792 Ingénierie des Systèmes Biologiques et des Procédés, F-31400 Toulouse, France.
Sophie
S
Barbe
Université de Toulouse; INSA,UPS,INP; LISBP, 135 Avenue de Rangueil, F-31077 Toulouse, France; CNRS, UMR5504, F-31400 Toulouse, France; INRA, UMR792 Ingénierie des Systèmes Biologiques et des Procédés, F-31400 Toulouse, France.
Claire
C
Moulis
Université de Toulouse; INSA,UPS,INP; LISBP, 135 Avenue de Rangueil, F-31077 Toulouse, France; CNRS, UMR5504, F-31400 Toulouse, France; INRA, UMR792 Ingénierie des Systèmes Biologiques et des Procédés, F-31400 Toulouse, France. Electronic address: remaud@insa-toulouse.fr.
Magali
M
Remaud-Siméon
D050260:Carbohydrate Metabolism; D019295:Computational Biology / Q000379:methods; D057225:Data Mining; D006801:Humans; D008958:Models, Molecular; D015202:Protein Engineering