Effect of fiber orientation of collagen-based electrospun meshes on human fibroblasts for ligament tissue engineering applications.
Within the past two decades polylactic-co-glycolic acid (PLGA) has gained considerable attention as a biocompatible and biodegradable polymer that is suitable for tissue engineering and regenerative medicine. In this present study, we have investigated the potential of PLGA, collagen I (ColI), and polyurethane (PU) scaffolds for ligament tissue regeneration. Two different ratios of PLGA (50:50 and 85:15) were used to determine the effects on mechanical tensile properties and cell adhesion. The Young's modulus, tensile stress at yield, and ultimate tensile strain of PLGA(50:50)-ColI-PU scaffolds demonstrated similar tensile properties to that of ligaments found in the knee. Whereas, scaffolds composed of PLGA(85:15)-ColI-PU had lower tensile properties than that of ligaments. Furthermore, we investigated the effect of fiber orientation on mechanical properties and our results indicate that aligned fiber scaffolds demonstrate higher tensile properties than scaffolds with random fiber orientation. Also, human fibroblasts attached and proliferated with no need for additional surface modifications to the presented electrospun scaffolds in both categories. Collectively, our investigation demonstrates the effectiveness of electrospun PLGA scaffolds as a suitable candidate for regenerative medicine, capable of being manipulated and combined with other polymers to create three-dimensional microenvironments with adjustable tensile properties to mimic native tissues.
Department of Surgery, Division Cardiothoracic Surgery, David Geffen School of Medicine, University of California, Los Angeles, California.
Sean Michael
SM
Full
Connor
C
Delman
Jessica M
JM
Gluck
Raushan
R
Abdmaulen
Richard J
RJ
Shemin
Sepideh
S
Heydarkhan-Hagvall
D024042:Collagen Type I / Q000737:chemistry*; D055119:Elastic Modulus; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry*; D008022:Ligaments; D008422:Materials Testing; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011140:Polyurethanes / Q000737:chemistry*; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
D024042:Collagen Type I; D011140:Polyurethanes; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid
collagen I; electrospinning; ligament tissue engineering; polylactic-co-glycolic acid; polyurethane