Cryptococcus terricola is a promising oleaginous yeast for biodiesel production from starch through consolidated bioprocessing.
Starch is considered a potential feedstock for biofuel production, particularly in light of the large-scale landfilling of food waste and other starchy materials worldwide. Lipid accumulation by oleaginous yeast is a promising method for biodiesel production from starch. However, most oleaginous yeasts are grown on monosaccharides or oligosaccharides because they cannot directly utilize starch. We therefore investigated the starch-assimilation ability of 1,200 yeasts. We found that Cryptococcus terricola could be used for fuel production through consolidated bioprocessing. C. terricola JCM 24523 exhibited the highest lipid content of 61.96% on medium with 5% starch at 10 days. Fatty acid methyl ester analysis showed that this strain produced high proportions of C16:0 and C18 fatty acids when grown on starch, which are ideal for use in biodiesel. Considering the yield and cost, lipids derived from starch using C. terricola would be a promising alternative source for biodiesel production.
Research Division of Microbial Sciences, Kyoto University, Kitashirakawa Oiwake-Cho, Sakyo-ku, Kyoto 606-8502, Japan.
Ayumi
A
Tanimura
Japan Collection of Microorganisms, RIKEN BioResource Center, Koyadai, Tuskuba, Ibaraki 305-0074, Japan.
Masako
M
Takashima
Department of Microbiology, Meiji Pharmaceutical University, Tokyo 204-8588, Japan.
Takashi
T
Sugita
Japan Collection of Microorganisms, RIKEN BioResource Center, Koyadai, Tuskuba, Ibaraki 305-0074, Japan.
Rikiya
R
Endoh
Research Division of Microbial Sciences, Kyoto University, Kitashirakawa Oiwake-Cho, Sakyo-ku, Kyoto 606-8502, Japan.
Minako
M
Kikukawa
Research Division of Microbial Sciences, Kyoto University, Kitashirakawa Oiwake-Cho, Sakyo-ku, Kyoto 606-8502, Japan.
Shino
S
Yamaguchi
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-Cho, Sakyo-ku, Kyoto 606-8502, Japan.
Eiji
E
Sakuradani
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-Cho, Sakyo-ku, Kyoto 606-8502, Japan.
Jun
J
Ogawa
Japan Collection of Microorganisms, RIKEN BioResource Center, Koyadai, Tuskuba, Ibaraki 305-0074, Japan.
Moriya
M
Ohkuma
1] Research Division of Microbial Sciences, Kyoto University, Kitashirakawa Oiwake-Cho, Sakyo-ku, Kyoto 606-8502, Japan [2].
Jun
J
Shima
D056804:Biofuels; D003454:Cryptococcus / Q000378:metabolism*; D003470:Culture Media; D004783:Environmental Microbiology; D005227:Fatty Acids / Q000378:metabolism; D007218:Industrial Microbiology; D007700:Kinetics; D050356:Lipid Metabolism; D013213:Starch / Q000378:metabolism*
D056804:Biofuels; D003470:Culture Media; D005227:Fatty Acids; D013213:Starch