Anti-nociceptive effect in mice of thillai flavonoid rutin.
We investigated the anti-nociceptive effect of Excoecaria agallocha (E.agallocha) against chemically and thermally induced nociception, Albino mice received a dose of 10, 15, 20, or 25 mg/kg of alkaline chloroform fraction (Alk-CF) of E.agallocha by oral administration. Compared with controls, Alk-CF decreased the writhing numbers (P<0.01) in a dose dependent manner. Further we determined that, Alk-CF contained, a potent compared to control, also potent anti-nociceptive agent that acted via opioid receptors and using HPLC, identified this compound as Rutin. Docking simulation demonstrated that Rutin interacted strongly with cyclooxygenase, forming a number of specific hydrogen bonds. In conclusion we have identified peripheral and central anti-nociceptive activities of E.agallocha that involve opioid receptor, and in which the active compound is Rutin.
Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai 608502, Tamil Nadu, India.
Gurudeeban
G
Selvaraj
Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai 608502, Tamil Nadu, India.
Satyavani
S
Kaliamurthi
Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai 608502, Tamil Nadu, India.
Ramanathan
R
Thirungnasambandam
Nandha College of Pharmacy and Research Institute, Erode, Tamil Nadu, India.
Lalitha
L
Vivekanandan
Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai 608502, Tamil Nadu, India.
Thangavel
T
Balasubramanian
D000700:Analgesics / Q000302:isolation & purification* / Q000627:therapeutic use; D000818:Animals; D004353:Drug Evaluation, Preclinical; D019665:Euphorbiaceae / Q000737:chemistry*; D005260:Female; D008297:Male; D051379:Mice; D010146:Pain / Q000188:drug therapy*; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000627:therapeutic use; D012431:Rutin / Q000302:isolation & purification* / Q000627:therapeutic use
D000700:Analgesics; D010936:Plant Extracts; D012431:Rutin