[Optimal extraction technology for genistein in Flemingia macrophylla with response surface method].
OBJECTIVE
To optimize extraction techniques for genistein in Flemingia macrophylla by using response surface method.


METHODS
Based on the single factor experiment, extraction temperature, extraction time and solvent ratio served as the response factor while the extraction rate of genistein served as the response value. Based on multiple linear regression and binomial expression, response surface method was employed to optimize the extracting process, and predicative analysis was conducted.


RESULTS
The effects of factors in the descending order were as follows: extraction temperature, extraction time and solvent ratio. The optimal extraction technology was as follows: extraction temperature 80 degrees C, extraction time 3.5 h and solvent ratio 20 mL/g. Under these conditions, the extraction rate of genistein reached 0.161 mg/g.


CONCLUSIONS
The results are agreed with model predictions. The technology can be used as the extraction process of genistein in Flemingia macrophylla.
Ying-Feng
YF
Niu
Zhong-Lian
ZL
Zhang
Hai-Tao
HT
Li
Yan-Hong
YH
Guan
Chao-Zhong
CZ
Peng
Li-Xia
LX
Zhang
D007887:Fabaceae / Q000737:chemistry*; D019833:Genistein / Q000737:chemistry / Q000302:isolation & purification*; D016014:Linear Models; D000432:Methanol / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D012997:Solvents / Q000737:chemistry; D013678:Technology, Pharmaceutical / Q000379:methods*; D013696:Temperature
D012997:Solvents; D019833:Genistein; D000432:Methanol