Identification of sex-linked DNA markers by AFLP in Portunus trituberculatus.
To investigate the mechanism of sex determination of crabs, amplified fragment-length polymorphism DNA analysis was applied to identify sex-specific markers for Portunus trituberculatus. Eleven pairs of primers were used to amplify DNA isolated from male and female crabs. A total of 481 and 499 fragments were amplified for females and males, respectively, and the ratios of polymorphic fragments were 20.59-65.52% and 27.27-62.22%, respectively. Distribution difference values between female and male groups for 10 polymorphisms were greater than 40%; Cluster analysis revealed that Nei's genetic distances for 57 polymorphisms clustered according to sex. The results indicate that a difference at the DNA level exists between female and male crabs and provide data for further study on sex determination.
Ocean and Fisheries College, Huaihai Institute of Technology, Lianyungang 222005, China. Electronic address: lai.xiaofang@163.com.
XiaoFang
X
Lai
Ocean and Fisheries College, Huaihai Institute of Technology, Lianyungang 222005, China.
Huan
H
Gao
Ocean and Fisheries College, Huaihai Institute of Technology, Lianyungang 222005, China.
Binlun
B
Yan
Ocean and Fisheries College, Huaihai Institute of Technology, Lianyungang 222005, China.
Wenhua
W
Yan
Ocean and Fisheries College, Huaihai Institute of Technology, Lianyungang 222005, China.
Shanrui
S
Shen
D054458:Amplified Fragment Length Polymorphism Analysis; D000818:Animals; D003386:Brachyura / Q000235:genetics*; D004247:DNA / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D005260:Female; D005819:Genetic Markers; D008297:Male; D021141:Nucleic Acid Amplification Techniques; D020641:Polymorphism, Single Nucleotide; D019849:Sex Determination Processes / Q000235:genetics*
D017931:DNA Primers; D005819:Genetic Markers; D004247:DNA
AFLP; Cluster analysis; Portunus trituberculatus; Sex-linked