Different exosome cargo from plasma/bronchoalveolar lavage in non-small-cell lung cancer.
Tumor-derived exosomes mediate tumorigenesis by facilitating tumor growth, metastasis, development of drug resistance, and immunosuppression. However, little is known about the exosomes isolated from bronchoalveolar lavage (BAL) in patients with lung neoplasm. Exosomes isolated in plasma and BAL from 30 and 75 patients with tumor and nontumor pathology were quantified by acetylcholinesterase activity and characterized by Western Blot, Electron Microscopy, and Nanoparticle Tracking Analysis. Differences in exosome cargo were analyzed by miRNA quantitative PCR in pooled samples and validated in a second series of patients. More exosomes were detected in plasma than in BAL in both groups (P < 0.001). The most miRNAs evaluated by PCR array were detected in tumor plasma, tumor BAL, and nontumor BAL pools, but only 56% were detected in the nontumor plasma pool. Comparing the top miRNAs with the highest levels detected in each pool, we found close homology only between the BAL samples of the two pathologies. In tumor plasma, we found a higher percentage of miRNAs with increased levels than in tumor BAL or in nontumor plasma. The data reveal differences between BAL and plasma exosome amount and miRNA content.
Department of Medical Oncology, University Hospital Puerta de Hierro Majadahonda, Madrid, Spain.
Marta
M
Rodríguez
Javier
J
Silva
Ana
A
López-Alfonso
María Belen
MB
López-Muñiz
Cristina
C
Peña
Gemma
G
Domínguez
Jose Miguel
JM
García
Ana
A
López-Gónzalez
Miriam
M
Méndez
Mariano
M
Provencio
Vanesa
V
García
Félix
F
Bonilla
D000230:Adenocarcinoma / Q000097:blood / Q000737:chemistry / Q000473:pathology*; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001769:Blood / Q000378:metabolism*; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology*; D002289:Carcinoma, Non-Small-Cell Lung / Q000097:blood / Q000737:chemistry / Q000473:pathology*; D002294:Carcinoma, Squamous Cell / Q000097:blood / Q000737:chemistry / Q000473:pathology*; D055354:Exosomes / Q000378:metabolism*; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000097:blood / Q000737:chemistry / Q000473:pathology*; D008297:Male; D035683:MicroRNAs / Q000378:metabolism; D008875:Middle Aged; D055815:Young Adult
D035683:MicroRNAs