A novel reassortant H2N3 influenza virus isolated from China.
OBJECTIVE
To analyze the genetic composition of a novel H2N3 virus isolate identified from a duck cage swab in a live poultry market (LPM) in 2009 in Guangdong province of China.


METHODS
PCR-positive specimens were inoculated into embryonated chicken eggs and subtyped by conventional RT-PCR. All segments of the virus A/environment/Guangdong/2/2009 were sequenced, and phylogenetic trees were constructed and analyzed.


RESULTS
The genes of this virus belong to Eurasian-lineage avian viruses. The virus is a reassortant with the HA gene from an H2N2 virus and the NA gene from an H5N3 virus. The PB1, PB2, and NP genes were from an H4N6 virus, the PA was from an H3N8 virus, the M gene was from an H1N3 virus, and the NS gene was from an H10N6 virus.


CONCLUSIONS
A novel avian-origin reassortant H2N3 influenza virus was detected in a live poultry market. Its potential impacts and evolution should be closely monitored.
Chinese National Influenza Center, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Key Laboratory of Surveillance and Early warning on Infectious Disease, Key Laboratory for Medical Virology, National Health and family planning Commission, Beijing 102206, China.
Xiao Dan
XD
Li
Chinese National Influenza Center, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Key Laboratory of Surveillance and Early warning on Infectious Disease, Key Laboratory for Medical Virology, National Health and family planning Commission, Beijing 102206, China.
Shu Mei
SM
Zou
Chinese National Influenza Center, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Key Laboratory of Surveillance and Early warning on Infectious Disease, Key Laboratory for Medical Virology, National Health and family planning Commission, Beijing 102206, China.
Ye
Y
Zhang
Chinese National Influenza Center, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Key Laboratory of Surveillance and Early warning on Infectious Disease, Key Laboratory for Medical Virology, National Health and family planning Commission, Beijing 102206, China.
Tian
T
Bai
Chinese National Influenza Center, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Key Laboratory of Surveillance and Early warning on Infectious Disease, Key Laboratory for Medical Virology, National Health and family planning Commission, Beijing 102206, China.
Rong Bao
RB
Gao
Center for Disease Control and Prevention of Guangdong Province, Guangzhou 510300, Guangdong, China.
Xin
X
Zhang
Center for Disease Control and Prevention of Guangdong Province, Guangzhou 510300, Guangdong, China.
Jie
J
Wu
Chinese National Influenza Center, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Key Laboratory of Surveillance and Early warning on Infectious Disease, Key Laboratory for Medical Virology, National Health and family planning Commission, Beijing 102206, China.
Yue Long
YL
Shu
D000818:Animals; D002681:China; D004372:Ducks / Q000821:virology; D016679:Genome, Viral; D009980:Influenza A virus / Q000235:genetics / Q000302:isolation & purification*; D005585:Influenza in Birds / Q000821:virology*; D010802:Phylogeny
Avian influenza; Gene analysis; H2N3