Spatial covariance reconstructive (SCORE) super-resolution fluorescence microscopy.
Super-resolution fluorescence microscopy has become a powerful tool to resolve structural information that is not accessible to traditional diffraction-limited imaging techniques such as confocal microscopy. Stochastic optical reconstruction microscopy (STORM) and photoactivation localization microscopy (PALM) are promising super-resolution techniques due to their relative ease of implementation and instrumentation on standard microscopes. However, the application of STORM is critically limited by its long sampling time. Several recent works have been focused on improving the STORM imaging speed by making use of the information from emitters with overlapping point spread functions (PSF). In this work, we present a fast and efficient algorithm that takes into account the blinking statistics of independent fluorescence emitters. We achieve sub-diffraction lateral resolution of 100 nm from 5 to 7 seconds of imaging. Our method is insensitive to background and can be applied to different types of fluorescence sources, including but not limited to the organic dyes and quantum dots that we demonstrate in this work.
Department of Physics, Princeton University, Princeton, New Jersey, United States of America.
Yi
Y
Deng
Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey, United States of America.
Mingzhai
M
Sun
Department of Physiology and Biophysics, Robert Wood Johnson Medical School, Piscataway, New Jersey, United States of America.
Pei-Hui
PH
Lin
Department of Physiology and Biophysics, Robert Wood Johnson Medical School, Piscataway, New Jersey, United States of America.
Jianjie
J
Ma
Department of Physics, Princeton University, Princeton, New Jersey, United States of America; Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey, United States of America.
Joshua W
JW
Shaevitz
D005456:Fluorescent Dyes; D008856:Microscopy, Fluorescence / Q000379:methods*; D013269:Stochastic Processes
D005456:Fluorescent Dyes