Pretreatment of lignocellulosic biomass using Fenton chemistry.
In an attempt to mimic white-rot fungi lignin degradation via in vivo Fenton chemistry, solution phase Fenton chemistry (10 g biomass, 176 mmol hydrogen peroxide and 1.25 mmol Fe(2+) in 200 mL of water) was applied to four different biomass feedstocks. An enzymatic saccharification of Fenton pretreated biomass showed an average 212% increase relative to untreated control across all four feedstocks (P<0.05, statistically significant). A microbial fermentation of the same Fenton pretreated biomass showed a threefold increase in gas production upon a sequential co-culture with Clostridium thermocellum and Clostridium beijerinckii. These results demonstrate the use of solution phase Fenton chemistry as a viable pretreatment method to make cellulose more bioavailable for microbial biofuel conversion.
University of Kentucky, Department of Chemistry, Lexington, KY 40506, United States.
Dawn M
DM
Kato
University of Kentucky, Department of Biosystems & Agricultural Engineering, Lexington, KY 40546, United States.
Noelia
N
Elía
USDA, Agricultural Research Service Forage-Animal Production Research Unit, Lexington, KY 40546, United States; University of Kentucky, Department of Animal and Food Sciences, Lexington, KY 40546, United States.
Michael
M
Flythe
University of Kentucky, Department of Chemistry, Lexington, KY 40506, United States. Electronic address: bclynn2@uky.edu.
Bert C
BC
Lynn
D018533:Biomass; D050260:Carbohydrate Metabolism; D002244:Carbon / Q000032:analysis; D002480:Cellulase / Q000378:metabolism; D046970:Clostridium beijerinckii / Q000378:metabolism; D048013:Acetivibrio thermocellus / Q000378:metabolism; D018920:Coculture Techniques; D005285:Fermentation; D006861:Hydrogen Peroxide / Q000737:chemistry* / Q000378:metabolism; D007501:Iron / Q000737:chemistry*; D008031:Lignin / Q000737:chemistry* / Q000378:metabolism; D006109:Poaceae / Q000737:chemistry; D012996:Solutions
C045076:Fenton's reagent; D012996:Solutions; C036909:lignocellulose; D002244:Carbon; D008031:Lignin; D006861:Hydrogen Peroxide; D007501:Iron; D002480:Cellulase
Fenton reagent; Lignocellulosic biomass; Microbial fermentation; Pretreatment