A new human IgG avidity test, using mixtures of recombinant antigens (rROP1, rSAG2, rGRA6), for the diagnosis of difficult-to-identify phases of toxoplasmosis.
The preliminary diagnostic utility of two mixtures of Toxoplasma gondii recombinant antigens (rROP1+rSAG2 and rROP1+rGRA6) in IgG ELISA and IgG avidity test has been evaluated. A total of 173 serum samples from patients with toxoplasmosis and seronegative people were examined. The sensitivity of IgG ELISA for rROP1+rSAG2 and rROP1+rGRA6 was 91.1% and 76.7%, respectively, while the reactivity for sera from patients where acute toxoplasmosis was suspected was higher, at 100% and 95.4%, respectively, than for people with chronic infection, at 88.2% and 70.6%. In this study a different trend in avidity maturation of IgG antibodies for two mixtures of proteins in comparison with native antigen was observed. The results suggest that a new IgG avidity test using the mixtures of recombinant antigens may be useful for the diagnosis of difficult-to-identify phases of toxoplasmosis. For this reason, selected mixtures after the additional tests on groups of sera with well-defined dates of infection could be used as a better alternative to the native antigens of the parasite in the serodiagnosis of human T. gondii infection.
Gdansk University of Technology, Chemical Faculty, Department of Microbiology, Gdańsk, Poland.
Dorota
D
Drapała
Gdansk University of Technology, Chemical Faculty, Department of Microbiology, Gdańsk, Poland. Electronic address: holec@pg.gda.pl.
Lucyna
L
Holec-Gąsior
Gdansk University of Technology, Chemical Faculty, Department of Microbiology, Gdańsk, Poland.
Józef
J
Kur
Gdansk University of Technology, Chemical Faculty, Department of Microbiology, Gdańsk, Poland.
Bartłomiej
B
Ferra
Gdansk University of Technology, Chemical Faculty, Department of Microbiology, Gdańsk, Poland; AgResearch, Lincoln, Private Bag 4749, New Zealand.
Elżbieta
E
Hiszczyńska-Sawicka
Medical University of Gdańsk, Departament of Obsterics, Gdańsk, Poland.
Dariusz
D
Lautenbach
D000913:Antibodies, Protozoan / Q000276:immunology*; D000915:Antibody Affinity; D000953:Antigens, Protozoan / Q000235:genetics; D019411:Clinical Laboratory Techniques / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D008565:Membrane Proteins / Q000235:genetics; D010274:Parasitology / Q000379:methods; D011247:Pregnancy; D015800:Protozoan Proteins / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics; D012680:Sensitivity and Specificity; D014122:Toxoplasma / Q000276:immunology*; D014123:Toxoplasmosis / Q000175:diagnosis* / Q000276:immunology
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; C095047:GRA6 protein, Toxoplasma gondii; D007074:Immunoglobulin G; D008565:Membrane Proteins; D015800:Protozoan Proteins; C117001:ROP 1 protein, Toxoplasma gondii; D011994:Recombinant Proteins; C067547:surface antigen P22, Toxoplasma
GRA6; IgG avidity; ROP1; Recombinant antigens; SAG2; Toxoplasmosis