Bolus tracking with nanofilter-based multispectral videography for capturing microvasculature hemodynamics.
Multispectral imaging is a highly desirable modality for material-based analysis in diverse areas such as food production and processing, satellite-based reconnaissance, and biomedical imaging. Here, we present nanofilter-based multispectral videography (nMSV) in the 700 to 950 nm range made possible by the tunable extraordinary-optical-transmission properties of 3D metallic nanostructures. Measurements made with nMSV during a bolus injection of an intravascular tracer in the ear of a piglet resulted in spectral videos of the microvasculature. Analysis of the multispectral videos generated contrast measurements representative of arterial pulsation, the distribution of microvascular transit times, as well as a separation of the venous and arterial signals arising from within the tissue. Therefore, nMSV is capable of acquiring serial multispectral images relevant to tissue hemodynamics, which may have application to the detection and identification of skin cancer.
1] Imaging Program, Lawson Health Research Institute, St. Joseph's Health Care, London, ON, Canada [2] The School of Engineering Science, Simon Fraser University, Burnaby, BC, Canada [3] Department of Medical Biophysics, Schulich School of Medicine and Dentistry, University of Western Ontario, London, ON, Canada.
Mohamadreza
M
Najiminaini
The School of Engineering Science, Simon Fraser University, Burnaby, BC, Canada.
Bozena
B
Kaminska
1] Imaging Program, Lawson Health Research Institute, St. Joseph's Health Care, London, ON, Canada [2] Department of Medical Biophysics, Schulich School of Medicine and Dentistry, University of Western Ontario, London, ON, Canada.
Keith
K
St Lawrence
1] Imaging Program, Lawson Health Research Institute, St. Joseph's Health Care, London, ON, Canada [2] Department of Medical Biophysics, Schulich School of Medicine and Dentistry, University of Western Ontario, London, ON, Canada.
Jeffrey J L
JJ
Carson
D006439:Hemodynamics; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D018715:Microscopy, Video; D055806:Microvessels / Q000503:physiopathology*; D012878:Skin Neoplasms / Q000175:diagnosis* / Q000473:pathology