Effects of caffeic acid phenethyl ester on wound healing of nasal mucosa in the rat: an experimental study.
OBJECTIVE
Wound healing of the nasal mucosa is a highly complex process that restores the anatomical and functional integrity of tissue that has been exposed to trauma. In this experimental study, our aim was to use histopathological examination to investigate the effects of caffeic acid phenethyl ester on the wound healing of rat nasal mucosa after mechanical trauma.


METHODS
The rats were randomly divided into 3 experimental groups: a non-treated group (n=7), a control saline group (n=7) and a caffeic acid phenethyl ester group (n=7). The non-treated group received no treatment for 15 days. The second group was administered saline (2.5 mL/kg, intraperitoneal) once a day for 15 days. The third group received caffeic acid phenethyl ester intraperitoneally at a dose of 10 μmol/kg once a day for 15 days. At the beginning of the study, unilateral mechanical nasal trauma was induced on the right nasal mucosa of all rats in the three groups using a brushing technique. Samples were stained using hematoxylin and eosin solution and were examined by a pathologist using a light microscope.


RESULTS
The severity of inflammation was milder in the caffeic acid phenethyl ester group compared with that in the non-treated and saline groups (P<0.05). The subepithelial thickness index was lower in the experimental group (P<0.05). Goblet cell and ciliated cell loss was substantially reduced in the experimental group compared with the non-treated and saline groups (P<0.05).


CONCLUSIONS
Caffeic acid phenethyl ester decreases inflammation and the loss of goblet cells and ciliated cells. Therefore, caffeic acid phenethyl ester has potential beneficial effects on the wound healing of nasal mucosa in the rat.
Department of ENT and Head and Neck Surgery, Dicle University Medical College, Diyarbakir, Turkey. Electronic address: vefakinis@hotmail.com.
Vefa
V
Kınıs
Department of ENT and Head and Neck Surgery, Dicle University Medical College, Diyarbakir, Turkey.
Musa
M
Ozbay
Department of ENT and Head and Neck Surgery, Dicle University Medical College, Diyarbakir, Turkey.
Mehmet
M
Akdag
Department of Pathology, Dicle University Medical College, Diyarbakir, Turkey.
Ulas
U
Alabalık
Department of ENT and Head and Neck Surgery, Dicle University Medical College, Diyarbakir, Turkey.
Aylin
A
Gul
Department of ENT and Head and Neck Surgery, Dicle University Medical College, Diyarbakir, Turkey.
Beyhan
B
Yılmaz
Department of ENT and Head and Neck Surgery, Dicle University Medical College, Diyarbakir, Turkey.
Hakan
H
Ozkan
Department of ENT and Head and Neck Surgery, Dicle University Medical College, Diyarbakir, Turkey.
Ismail
I
Topcu
D000818:Animals; D002109:Caffeic Acids / Q000008:administration & dosage*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D007274:Injections, Intraperitoneal; D008297:Male; D016328:NF-kappa B / Q000037:antagonists & inhibitors; D009297:Nasal Mucosa / Q000187:drug effects / Q000293:injuries / Q000473:pathology*; D010626:Phenylethyl Alcohol / Q000008:administration & dosage / Q000031:analogs & derivatives*; D051381:Rats; D017208:Rats, Wistar; D016896:Treatment Outcome; D014945:Wound Healing / Q000187:drug effects*; D014947:Wounds and Injuries / Q000188:drug therapy* / Q000473:pathology
D002109:Caffeic Acids; D016328:NF-kappa B; C055494:caffeic acid phenethyl ester; D010626:Phenylethyl Alcohol