Matrix segregation as the major cause for sample inhomogeneity in MALDI dried droplet spots.
The segregation in dried droplet MALDI sample spots was analyzed with regard to the matrix-to-sample ratio using optical microscopy, MALDI imaging mass spectrometry (MALDI MSI) and IR imaging spectroscopy. In this context, different polymer/matrix/solvent systems usually applied in the analysis of synthetic polymers were investigated. The use of typical matrix concentrations (10 mg mL⁻¹) in almost every case resulted in ring patterns, whereas higher concentrated matrix solutions always led to homogeneous sample spot layers. The data revealed that segregation is predominantly caused by matrix transport in the drying droplet, whereas polymer segregation seems to be only secondary.
Federal Institute for Materials Research and Testing (BAM), D-12489, Berlin, Germany.
Stefan J
SJ
Gabriel
Clemens
C
Schwarzinger
Bettina
B
Schwarzinger
Ulrich
U
Panne
Steffen M
SM
Weidner
D000097:Acetonitriles / Q000737:chemistry; D055664:Electrochemical Techniques; D005663:Furans / Q000737:chemistry; D005841:Gentisates / Q000737:chemistry; D008567:Membranes, Artificial; D008853:Microscopy; D008958:Models, Molecular; D008970:Molecular Weight; D009570:Nitriles / Q000737:chemistry; D061848:Optical Imaging; D011092:Polyethylene Glycols / Q000737:chemistry*; D019904:Polymethyl Methacrylate / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry*; D015203:Reproducibility of Results; D012997:Solvents / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013055:Spectrophotometry, Infrared; D014212:Tretinoin / Q000737:chemistry; D014835:Volatilization
C555195:2-((2E)-3-(4-tert-butylphenyl)-2-methylprop-2-enylidene)malononitrile; D000097:Acetonitriles; D005663:Furans; D005841:Gentisates; D008567:Membranes, Artificial; D009570:Nitriles; D011137:Polystyrenes; D012997:Solvents; C018674:tetrahydrofuran; D011092:Polyethylene Glycols; D014212:Tretinoin; D019904:Polymethyl Methacrylate; C010925:2,5-dihydroxybenzoic acid; C032159:acetonitrile