The effect of ginger extract on glycoproteins of Raji cells.
Protein glycosylation is associated with the development and progression of specific diseases, including cancers. The ginger rhizome is known to have anti-cancer and anti-fungal properties. This investigation was carried out to study the effect of ginger on glycoproteins of Raji cells. A 10% yield of ginger extract was mixed with 0.01% DMSO and added to 6 x 10(4) Raji cells at different concentrations for 24, 48 and 72 h at 37 degrees C. Their half maximal inhibitory concentration (IC50) was determined and analyzed statistically using Graphpad prism software. Cell extracts were prepared and their glycoproteins purified using lectin-affinity chromatography (Q proteome total glycoprotein and O glycoprotein kits) and SDS PAGE was carried out. IC50 of ginger extract on Raji cells was 20 microg mL(-1) at 72 h with < 0.01 significance. Silver staining of purified glycoprotiens in Raji cells indicated the presence of O-glycans and N-glycans. N-linked mannose and N-linked sialic acids were detected with the total glycoprotein kit. O-linked galactose and O-linked sialic acids were identified with the O-glycoprotein. Ginger reduced the expression of O-linked sialic acid and also N-linked mannose on Raji cells but had no effect on other glycoproteins. Sialic acid is now well known as a cancer marker and investigations are on to use it as a drug-target in cancerous tissues.
Zahra
Z
Zamani
Nassir-Ud-Din
Haleemeh Kabini
HK
Kohan
Mehdi
M
Kadivar
Zahra
Z
Kalyee
Behzad Laame
BL
Rad
Ayda
A
Iravani
Nourooz Ali
NA
Rahimi
Farideh
F
Wahabi
Sedigheh
S
Sadeghi
Fatemeh
F
Pourfallah
Mohammad
M
Arjmand
D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D020939:Zingiber officinale / Q000737:chemistry*; D006023:Glycoproteins / Q000737:chemistry*; D006801:Humans; D059828:Plant Cells / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*
D006023:Glycoproteins; D010936:Plant Extracts