Collagen type III alpha 1 polymorphism (rs1800255, COL3A1 2209 G>A) assessed with high-resolution melting analysis is not associated with pelvic organ prolapse in the Dutch population.
OBJECTIVE
The rs1800255, COL3A1 2209 G>A polymorphism in the alpha 1 chain of collagen type III has been associated with an increased risk of pelvic organ prolapse (POP). In one of our previous studies however, polymerase chain reaction-based restriction fragment length polymorphism (PCR-RFLP) misdiagnosed rs1800255, COL3A1 2209 G>A in 6 % of cases. The high-resolution melting (HRM) analysis on the contrary obtained a 100 % accordance for this specific polymorphism and was used in the present study to validate this risk factor for POP.


METHODS
In this case-control study, women with and without symptoms of POP were included and compared. DNA was extracted from blood samples. HRM analysis was used to assess for the presence of the homozygous rs1800255. Groups were compared using the Pearson chi-square, Mann-Whitney, and t tests. The discrepancy between HRM and PCR-RFLP results was investigated using PCR-RFLP results available from our previous study.


RESULTS
The study included 354 women: 272 patients with POP and 82 controls; 18 (7 %) cases versus 3 (4 %) controls had a homozygous rs1800255, COL3A1 2209 G>A polymorphism (odds ratio 1.9, 95 % confidence interval 0.5-6.9, compared to the wild type), and thus no association between POP and the homozygous polymorphism could be demonstrated. A discrepancy between HRM and PCR-RFLP results was found in 8 % of the samples.


CONCLUSIONS
The previously found statistically significant association between the rs1800255, COL3A1 2209 G>A polymorphism as measured with PCR-RFLP and POP could no longer be demonstrated. This raises concerns regarding the results of other association studies using PCR-RFLP.
Department of Obstetrics and Gynecology (791), Radboud University Medical Center, P.O. Box 9101, 6500 HB, Nijmegen, The Netherlands, sabrinalince@yahoo.com.
Sabrina L
SL
Lince
Leon C
LC
van Kempen
Jeroen R
JR
Dijkstra
Joanna
J
IntHout
Mark E
ME
Vierhout
Kirsten B
KB
Kluivers
D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D015331:Cohort Studies; D024061:Collagen Type III / Q000235:genetics*; D005260:Female; D006801:Humans; D008875:Middle Aged; D009426:Netherlands; D009691:Nucleic Acid Denaturation; D056887:Pelvic Organ Prolapse / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide
C439971:COL3A1 protein, human; D024061:Collagen Type III