Comparative evaluation of the Diagenode multiplex PCR assay on the BD max system versus a routine in-house assay for detection of Bordetella pertussis.
This study looked at 128 nasopharyngeal aspirates (NPA) and 162 throat swabs (TS) tested with the Diagenode multiplex assay on the BD Max system versus our in-house Bordetella pertussis PCR. Sensitivity and specificity were 97.3% and 100% for NPA and 88.3% and 98% for TS, respectively. Of positive NPA, 42.1% were coinfected with respiratory viruses.
Department of Medical Microbiology, Royal Infirmary of Edinburgh, Edinburgh, United Kingdom.
Juliet
J
Kenicer
Department of Medical Microbiology, Royal Infirmary of Edinburgh, Edinburgh, United Kingdom.
Alison
A
Hardie
Department of Medical Microbiology, Royal Infirmary of Edinburgh, Edinburgh, United Kingdom.
Fiona
F
Hamilton
Department of Medical Microbiology, Royal Infirmary of Edinburgh, Edinburgh, United Kingdom.
Naomi
N
Gadsby
Department of Medical Microbiology, Royal Infirmary of Edinburgh, Edinburgh, United Kingdom Kate.Templeton@luht.scot.nhs.uk.
Kate
K
Templeton
D000293:Adolescent; D000328:Adult; D000369:Aged, 80 and over; D057205:Automation, Laboratory / Q000379:methods; D001886:Bordetella pertussis / Q000235:genetics / Q000302:isolation & purification*; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008875:Middle Aged; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D009305:Nasopharynx / Q000382:microbiology; D010614:Pharynx / Q000382:microbiology; D012680:Sensitivity and Specificity; D014917:Whooping Cough / Q000175:diagnosis*; D055815:Young Adult