Effect of platelet releasate on osteogenic differentiation of human mesenchymal bone marrow stem cells.
We studied the effect of platelet releasate on osteogenic differentiation of human mesenchymal BM stem cells. Histological staining and reverse transcription PCR analysis showed that addition of platelet releasate to osteogenic differentiation medium stimulated the formation of calcium ossificates and alkaline phosphatase and increased expression of osteopontin and alkaline phosphatase genes.
Republican Research and Practical Center of Transfusion and Medical Biotechnologies, Minsk, Belarus, 4kosmacheva@mail.ru.
S M
SM
Kosmacheva
N N
NN
Danilkovich
A V
AV
Shchepen'
S I
SI
Ignatenko
M P
MP
Potapnev
D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism; D001792:Blood Platelets / Q000502:physiology*; D002454:Cell Differentiation; D002457:Cell Extracts; D002478:Cells, Cultured; D004790:Enzyme Induction; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D010012:Osteogenesis; D053495:Osteopontin / Q000235:genetics / Q000378:metabolism
D002457:Cell Extracts; D053495:Osteopontin; D000469:Alkaline Phosphatase