[Effects of rhG-CSF mobilization on the polarization and migration of donor's CD4(+)T lymphocytes].
The polarization and migration of T lymphocytes involves the adhesive interaction of lymphocyte function-associated antigen 1(LFA-1) with its ligand intercellular adhesion molecule 1 (ICAM-1). This study was aimed to investigate the effects of recombinant human granulocyte colony-stimulating factor (rhG-CSF) mobilization on the polarization and migration of donor's CD4(+) T cells in peripheral blood. The peripheral blood mononuclear cells were collected from 10 healthy volunteers and 10 donors on the fifth day of mobilization with rhG-CSF. And the CD4(+)T cells were purified by miniMACS. The polarization and migration of CD4(+) T cells activated by stroma cell-derived factor -1α (SDF-1α) and ICAM-1 were detected by using inverted and confocal microscopes respectively. The results showed that the percentage of polarized CD4(+)T cells from donors(32.42 ± 4.91)% was lower than that from healthy controls(56.55 ± 5.35)% (P < 0.01), the migration velocity of CD4(+)T cells from donors (7.06 ± 1.44 µm/min) was also lower than that of healthy controls(9.05 ± 1.91 µm/min)(P < 0.01). It is concluded that the polarization and migration of CD4(+)T lymphocytes is impaired after rhG-CSF mobilization.
Medical College of Chinese PLA, Department of Hematology, Chinese PLA General Hospital, Beijing 100853, China.
Zhen-Yang
ZY
Gu
Medical College of Chinese PLA, Department of Hematology, Chinese PLA General Hospital, Beijing 100853, China.
Meng
M
Li
Medical College of Chinese PLA, Department of Hematology, Chinese PLA General Hospital, Beijing 100853, China.
Sha-Sha
SS
Zhao
Medical College of Chinese PLA, Department of Hematology, Chinese PLA General Hospital, Beijing 100853, China.
Bo
B
Cai
Medical College of Chinese PLA, Department of Hematology, Chinese PLA General Hospital, Beijing 100853, China.
Xiao-Li
XL
Zhao
Medical College of Chinese PLA, Department of Hematology, Chinese PLA General Hospital, Beijing 100853, China.
Li-Xun
LX
Guan
Medical College of Chinese PLA, Department of Hematology, Chinese PLA General Hospital, Beijing 100853, China.
Li-Li
LL
Wang
Medical College of Chinese PLA, Department of Hematology, Chinese PLA General Hospital, Beijing 100853, China. E-mail: gaochunji@hotmail.com.
Chun-Ji
CJ
Gao
D000293:Adolescent; D000328:Adult; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology* / Q000187:drug effects; D016022:Case-Control Studies; D002465:Cell Movement; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011994:Recombinant Proteins / Q000494:pharmacology; D014019:Tissue Donors; D055815:Young Adult
D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor