Evaluation of hexane and ethyl acetate extracts of the sponge Jaspis diastra collected from Mauritius Waters on HeLa cells.
OBJECTIVE
Based on previous screening results, the cytotoxic effect of the hexane (JDH) and ethyl acetate extracts (JDE) of the marine sponge Jaspis diastra were evaluated on HeLa cells and the present study aimed at determining their possible mechanism of cell death.


METHODS
Nuclear staining, membrane potential change, flow cytometry analysis of cell cycle distribution and annexin V staining were undertaken to investigate the effects of JDE and JDH. Electrospray ionization mass spectrometry (ESI-MS) and nuclear magnetic resonance were used to characterize an isolated bioactive molecule.


RESULTS
JDE displayed an IC50 25 times more significant than the JDH. Flow cytometry analysis revealed JDE induced apoptosis in HeLa cells accompanied by the collapse of mitochondrial membrane potential. Fractionation of JDE resulted in the isolation of the known cytotoxic cyclodepsipeptide, Jaspamide.


CONCLUSIONS
Taking our results together suggest that JDE can be valuable for the development of anticancer drugs, especially for cervical cancer. Further investigations are currently in progress with the aim to determine and isolate other bioactive compounds from this extract.
Mauritius Oceanography Institute (MOI), Quatre-Bornes, Mauritius.
Girish
G
Beedessee
Avin
A
Ramanjooloo
Inés
I
Tiscornia
Thierry
T
Cresteil
Srinivasarao
S
Raghothama
Deepak
D
Arya
Shashanka
S
Rao
Konkallu Hanumae
KH
Gowd
Mariela
M
Bollati-Fogolin
Daniel E P
DE
Marie
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D017209:Apoptosis / Q000187:drug effects; D001688:Biological Products / Q000494:pharmacology / Q000627:therapeutic use*; D047630:Depsipeptides / Q000494:pharmacology / Q000627:therapeutic use*; D005260:Female; D006367:HeLa Cells; D006801:Humans; D008436:Mauritius; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D011161:Porifera / Q000737:chemistry*; D002583:Uterine Cervical Neoplasms / Q000188:drug therapy*
D000970:Antineoplastic Agents; D001688:Biological Products; D047630:Depsipeptides; C057531:jasplakinolide
HeLa cells; Jaspis diastra; Mauritius; cytotoxic; sponges