Quantitative T2* (T2 star) relaxation times predict site specific proteoglycan content and residual mechanics of the intervertebral disc throughout degeneration.
Degeneration alters the biochemical composition of the disc, affecting the mechanical integrity leading to spinal instability. Quantitative T2* MRI probes water mobility within the macromolecular network, a potentially more sensitive assessment of disc health. We determined the relationship between T2* relaxation time and proteoglycan content, collagen content, and compressive mechanics throughout the degenerative spectrum. Eighteen human cadaveric lumbar (L4-L5) discs were imaged using T2* MRI. The T2* relaxation time at five locations (nucleous pulposus or NP, anterior annulus fibrosis or AF, posterior AF, inner AF, and outer AF) was correlated with sulfated-glycosaminoglycan (s-GAG) content, hydroxyproline content, and residual stress and strain at each location. T2* relaxation times were significantly correlated with s-GAG contents in all test locations and were particularly strong in the NP (r = 0.944; p < 0.001) and inner AF (r = 0.782; p < 0.001). T2* relaxation times were also significantly correlated with both residual stresses and excised strains in the NP (r = 0.857; p < 0.001: r = 0.816; p < 0.001), inner AF (r = 0.535; p = 0.022: r = 0.516; p = 0.028), and outer AF (r = 0.668; p = 0.002: r = 0.458; p = 0.041). These strong correlations highlight T2* MRI's ability to predict the biochemical and mechanical health of the disc. T2* MRI assessment of disc health is a clinically viable tool showing promise as a biomarker for distinguishing degenerative changes.
Department of Biomedical Engineering, University of Minnesota, Nils Hasselmo Hall, Room 7-105, 312 Church Street S.E., Minneapolis, Minnesota.
Arin M
AM
Ellingson
Tina M
TM
Nagel
David W
DW
Polly
Jutta
J
Ellermann
David J
DJ
Nuckley
D000328:Adult; D000368:Aged; D001696:Biomechanical Phenomena; D002102:Cadaver; D003094:Collagen; D006025:Glycosaminoglycans / Q000032:analysis; D006801:Humans; D007403:Intervertebral Disc / Q000737:chemistry*; D055959:Intervertebral Disc Degeneration / Q000473:pathology / Q000503:physiopathology*; D008159:Lumbar Vertebrae / Q000737:chemistry; D008279:Magnetic Resonance Imaging / Q000379:methods; D008875:Middle Aged; D011509:Proteoglycans / Q000032:analysis*; D014867:Water
D006025:Glycosaminoglycans; D011509:Proteoglycans; D014867:Water; C013786:A73025; D003094:Collagen
T2* (T2 star); biomechanics; disc degeneration; proteoglycan; quantitative magnetic resonance imaging