Synthesis, cellular uptake, and biodistribution of whey-rich nanoparticles.
Whey-poly(acrylic acid) (whey-PAA) nanoparticles are prepared by polymerizing acrylic acid (AA) monomer in the presence of whey protein in a complete aqueous medium. The properties, drug loading, and release as well as in vitro cytotoxicity of whey-PAA nanoparticles are examined. The cellular uptakes and penetration of nanoparticle in the SH-SY5Y monolayer cells and multicellular tumor spheroids are observed. The in vivo distribution of the nanoparticles in tumor-bearing mice is evaluated. Confocal laser scanning microscopy and co-localization images show that the nanoparticles are well internalized by the cells through the endocytosis mechanism. Drug-loaded whey-PAA nanoparticles can penetrate multicellular tumor spheroids more deeply. In vivo near-infrared fluorescence imaging examination and in vivo DOX distribution show that the drug-loaded whey-PAA nanoparticles can well accumulated in the tumor site. Thus, these whey-rich nanoparticles seem to be very promising drug carriers for drug delivery.
Department of Polymer Science and Engineering, College of Chemistry and Chemical Engineering, Jiangsu Key Laboratory for Nanotechnology, Nanjing University, Nanjing, 210093, P. R. China.
Xu
X
Zhen
Xin
X
Wang
Chenchen
C
Yang
Qin
Q
Liu
Wei
W
Wu
Baorui
B
Liu
Xiqun
X
Jiang
D000180:Acrylic Resins / Q000032:analysis*; D000704:Analysis of Variance; D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry*; D004317:Doxorubicin; D016503:Drug Delivery Systems / Q000379:methods*; D007700:Kinetics; D046911:Macromolecular Substances / Q000138:chemical synthesis* / Q000737:chemistry; D051379:Mice; D018613:Microscopy, Confocal; D008894:Milk Proteins / Q000032:analysis*; D053758:Nanoparticles / Q000737:chemistry* / Q000378:metabolism; D061848:Optical Imaging; D000067816:Whey Proteins
D000180:Acrylic Resins; D000970:Antineoplastic Agents; D046911:Macromolecular Substances; D008894:Milk Proteins; D000067816:Whey Proteins; C006903:carbopol 940; D004317:Doxorubicin
cellular uptake; drug delivery; protein nanoparticles; whey