A novel single-labeled fluorescent oligonucleotide probe for silver(I) ion detection in water, drugs, and food.
Due to the high toxicity of silver(I) ions, a method for the rapid, sensitive, and selective detection for silver(I) ions in water, pharmaceutical products, and food is of great importance. Herein, a novel single-labeled fluorescent oligonucleotide (OND) probe based on cytosine-Ag(I)-cytosine coordination and the inherent fluorescence quenching ability of the G-quadruplex is designed to detect silver(I) ions. The formation of a hairpin structure in the OND-Ag(I) complex brings the hexachloro fluorescein (HEX) labeled at the 5'-end of the OND probe close to the G-quadruplex located at the 3'-end of the OND probe, leading to a fluorescence quenching due to photoinduced electron transfer between HEX and the G-quadruplex. Through this method, silver(I) ions can be detected quantitatively, the linear response range is from 1 to 100 nmol/L with a detection limit of 50 pmol/L, and no obvious interference occurs with other metal ions with a 10-fold concentration. This assay is simple, sensitive, and selective, and it can be used to detect silver(I) ions in actual water, drug, and food samples.
College of Life Science, Northwest University , Xi'an, Shaanxi 710069, China.
Liujiao
L
Bian
Xu
X
Ji
Wei
W
Hu
D015374:Biosensing Techniques / Q000379:methods*; D004340:Drug Contamination; D005453:Fluorescence; D005506:Food Contamination / Q000032:analysis*; D057230:Limit of Detection; D015345:Oligonucleotide Probes / Q000737:chemistry*; D004364:Pharmaceutical Preparations / Q000032:analysis*; D012834:Silver / Q000032:analysis*; D014874:Water Pollutants, Chemical / Q000032:analysis*
D015345:Oligonucleotide Probes; D004364:Pharmaceutical Preparations; D014874:Water Pollutants, Chemical; D012834:Silver