A label-free electrochemical aptasensor for the analysis of the potassium ion.
Herein, a simple and novel electrochemical method for the detection of potassium ions (K(+)) was developed. In the presence of potassium ions, the potassium ions aptamer will form a G-quadruplex complex. Thus, further addition of hemin in the presence of potassium ions will lead to the formation of a recombined G-quadruplex. Then the electroactive label, hemin, will give an electrochemical response. The linear range of the method covered a large variation of K(+) concentration from 0.1 nM to 0.1 μ M and the detection limit of 0.1 nM was obtained. Moreover, this assay was able to detect K(+) with high selectivity and had great potential applications.
a Department of ICU , The Second People's Hospital of Wuxi , Wuxi , China.
Xiaoxia
X
Ji
Jialei
J
Li
Chengjian
C
Yang
D052157:Aptamers, Nucleotide; D015374:Biosensing Techniques; D055664:Electrochemical Techniques; D004566:Electrodes; D054856:G-Quadruplexes; D006046:Gold; D006427:Hemin / Q000737:chemistry; D011188:Potassium / Q000032:analysis*
D052157:Aptamers, Nucleotide; D006427:Hemin; D006046:Gold; D011188:Potassium
G-quadruplex; ICU; electrochemistry; potassium ions