Interleukin-1beta -511T>C genetic variant contributes to recurrent pregnancy loss risk and peripheral natural killer cell proportion.
OBJECTIVE
To identify whether interleukin gene polymorphisms are risk factors for idiopathic recurrent pregnancy loss (RPL) in Korean women.


METHODS
Case-control study.


METHODS
Hospital-based study.


METHODS
A cohort of 385 idiopathic RPL patients and 232 controls with Korean ethnicity.


METHODS
None.


METHODS
Genotyping was assessed with a polymerase chain reaction-restriction fragment length polymorphism assay. We examined polymorphisms in three interleukin (IL) genes: IL-1β, IL-4, and IL-10.


RESULTS
The IL-1β -511T>C polymorphism was associated with RPL (-511TT vs. -511CC: adjusted odds ratio 1.826; 95% confidence interval 1.130-2.953). Allelic gene-gene interaction analysis revealed that the T/B2/G (IL-1β/IL-4/IL-10) allele combination was only detected in the RPL group (adjusted odds ratio 20.046; 95% confidence interval 1.188-338.204). The proportion of peripheral natural killer cells was higher in patients with the IL-1β -511C allele compared with the -511T allele.


CONCLUSIONS
According to these results, IL-1β -511T>C may be a predisposing factor to RPL susceptibility. However, the mechanism underlying the function of IL-1β -511T>C in RPL remains to be determined, and further studies are needed to improve understanding of the roles of IL-1β -511T>C, using a larger and more heterogeneous cohort.
Institute for Clinical Research, CHA Bundang Medical Center, CHA University, Seongnam, South Korea; Department of Biomedical Science, College of Life Science, CHA University, Seongnam, South Korea.
Jung O
JO
Kim
Fertility Center of CHA Gangnam Medical Center, CHA University, Seoul, South Korea.
Woo Sik
WS
Lee
Institute for Clinical Research, CHA Bundang Medical Center, CHA University, Seongnam, South Korea; Department of Biomedical Science, College of Life Science, CHA University, Seongnam, South Korea.
Bo Eun
BE
Lee
Institute for Clinical Research, CHA Bundang Medical Center, CHA University, Seongnam, South Korea; Department of Biomedical Science, College of Life Science, CHA University, Seongnam, South Korea.
Young Joo
YJ
Jeon
Department of Obstetrics and Gynecology, CHA Bundang Medical Center, CHA University, Seongnam, South Korea.
Young Ran
YR
Kim
Department of Obstetrics and Gynecology, CHA Bundang Medical Center, CHA University, Seongnam, South Korea.
Sang Hee
SH
Jung
Department of Obstetrics and Gynecology, CHA Bundang Medical Center, CHA University, Seongnam, South Korea.
Sung Woon
SW
Chang
Institute for Clinical Research, CHA Bundang Medical Center, CHA University, Seongnam, South Korea; Department of Biomedical Science, College of Life Science, CHA University, Seongnam, South Korea. Electronic address: nkkim@cha.ac.kr.
Nam Keun
NK
Kim
D000026:Abortion, Habitual / Q000235:genetics* / Q000276:immunology; D000328:Adult; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics; D053583:Interleukin-1beta / Q000235:genetics*; D015847:Interleukin-4 / Q000235:genetics; D007694:Killer Cells, Natural / Q000276:immunology*; D018655:Lymphocyte Count; D016017:Odds Ratio; D010641:Phenotype; D011110:Polymorphism, Genetic; D011247:Pregnancy; D056910:Republic of Korea; D012307:Risk Factors
C508609:IL10 protein, human; C508598:IL4 protein, human; D053583:Interleukin-1beta; D016753:Interleukin-10; D015847:Interleukin-4
IL-1β -511T>C; NK cell; RPL; polymorphism; recurrent pregnancy loss