Stability of Lactobacillus rhamnosus GG in prebiotic edible films.
The concept of prebiotic edible films as effective vehicles for encapsulating probiotic living cells is presented. Four soluble fibres (inulin, polydextrose, glucose-oligosaccharides and wheat dextrin) were selected as prebiotic co-components of gelatine based matrices plasticised with glycerol and used for the immobilisation of Lactobacillus rhamnosus GG. The addition of prebiotics was associated with a more compact and uniform film structure, with no detectable interspaces or micropores; probiotic inclusion did not significantly change the structure of the films. Glucose-oligosaccharides and polydextrose significantly enhanced L. rhamnosus GG viability during air drying (by 300% and 75%, respectively), whilst a 33% and 80% reduction in viable counts was observed for inulin and wheat dextrin. Contrarily, inulin was the most effective at controlling the sub-lethal effects on L. rhamnosus GG during storage. However, in all cases the supplementation of edible films with prebiotics ameliorated the storage stability of L. rhamnosus GG.
Division of Food Sciences, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough LE12 5RD, Leicestershire, United Kingdom.
Christos
C
Soukoulis
Division of Food Sciences, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough LE12 5RD, Leicestershire, United Kingdom.
Solmaz
S
Behboudi-Jobbehdar
Division of Food Sciences, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough LE12 5RD, Leicestershire, United Kingdom.
Lina
L
Yonekura
Nottingham Nanotechnology and Nanoscience Centre, University of Nottingham, University Park, Nottingham NG7 2RD, Nottinghamshire, United Kingdom.
Christopher
C
Parmenter
Division of Food Sciences, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough LE12 5RD, Leicestershire, United Kingdom. Electronic address: Ian.Fisk@nottingham.ac.uk.
Ian D
ID
Fisk
D004337:Drug Carriers / Q000737:chemistry*; D016503:Drug Delivery Systems / Q000295:instrumentation*; D005936:Glucans / Q000737:chemistry; D007444:Inulin / Q000737:chemistry; D052201:Lacticaseibacillus rhamnosus / Q000737:chemistry*; D009844:Oligosaccharides / Q000737:chemistry; D056692:Prebiotics / Q000032:analysis / Q000382:microbiology*; D019936:Probiotics / Q000737:chemistry* / Q000494:pharmacology
D004337:Drug Carriers; D005936:Glucans; D009844:Oligosaccharides; D056692:Prebiotics; D007444:Inulin; C033375:polydextrose
Edible films; Encapsulation; Probiotics; Survival; Synbiotics