Molecular detection of nine clinically important non-O157 Escherichia coli serogroups from raw sheep meat in Chaharmahal-va-Bakhtiari province, Iran.
STEC isolates and also stx-negative Escherichia coli isolates from sheep meat from the Chaharmahal-va-Bakhtiari province, Iran were analyzed for nine clinically important non-O157 serotypes by PCR. A total of 90 E. coli isolates were tested. Stx-positive and eae-positive E. coli isolates did not belong to the nine most clinically relevant non-O157 STECs. Of the 80 non-STEC isolates, two belonged to the O103 and two belonged to the O128 groups. Stx-negative E. coli O103 and O128 strains isolated have potential in acquiring stx genes and continuing into the digestive system of consumers. Further studies are needed to analyze virulence characteristics of these E. coli strains to determine their potential role in causing disease in humans. For the sake of public health, it is important to monitor and investigate non-O157 diarrheagenic E. coli strains in meat in order to control and prevent them.
Faculty of Veterinary Medicine, University of Shahrekord, Shahrekord, Iran; Research Institute of Zoonotic Diseases, University of Shahrekord, Shahrekord, Iran. Electronic address: h.tahmasby@yahoo.com.
Hossein
H
Tahmasby
Faculty of Veterinary Medicine, University of Shahrekord, Shahrekord, Iran; Research Institute of Zoonotic Diseases, University of Shahrekord, Shahrekord, Iran. Electronic address: samanehmehrabiyan@yahoo.com.
Samaneh
S
Mehrabiyan
Department of Microbiology, Faculty of Basic Sciences, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran. Electronic address: ee_tajbakhsh@iaushk.ac.ir.
Elahe
E
Tajbakhsh
Faculty of Veterinary Medicine, University of Shahrekord, Shahrekord, Iran. Electronic address: sh.farahmandi@yahoo.com.
Sharmin
S
Farahmandi
Faculty of Veterinary Medicine, University of Shahrekord, Shahrekord, Iran. Electronic address: hadi.monji@yahoo.com.
Hadi
H
Monji
Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Electronic address: k.farahmandi@yahoo.com.
Keyvan
K
Farahmandi
D018829:Adhesins, Bacterial / Q000235:genetics; D000818:Animals; D004032:Diet; D004926:Escherichia coli / Q000235:genetics*; D019453:Escherichia coli O157 / Q000235:genetics; D029968:Escherichia coli Proteins / Q000235:genetics*; D005516:Food Microbiology; D005798:Genes, Bacterial; D006801:Humans; D007492:Iran; D008460:Meat / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D065288:Serogroup; D012703:Serotyping; D012756:Sheep; D022621:Shiga Toxin / Q000235:genetics*; D054323:Shiga-Toxigenic Escherichia coli / Q000235:genetics
D018829:Adhesins, Bacterial; D029968:Escherichia coli Proteins; C073819:eaeA protein, E coli; D022621:Shiga Toxin
Escherichia coli; Meat; PCR; Sheep; Shiga toxin