Diethylaminoethyl (DEAE) binding fraction from Taenia solium metacestode improves the neurocysticercosis serodiagnosis.
Neurocysticercosis (NC) is one of the most important diseases caused by parasites affecting the central nervous system. We fractionated by ion-exchange chromatography using diethylaminoethyl (DEAE)-sepharose resin the total saline extract (S) from Taenia solium metacestodes and evaluated obtained fractions (DEAE S1 and DEAE S2) by enzyme-linked immunosorbent assay (ELISA, n = 123) and immunoblotting (IB, n = 22) to detect human NC in serum. Diagnostic parameters were established by ROC and TG ROC curves for ELISA tests. IB was qualitatively analyzed. S and DEAE S1 presented sensitivity of 87. 5% and DEAE S2 90%. The best specificity was observed for DEAE S2 (90.4%). In IB, using DEAE S2 samples from NC patients presented bands of 20-25, 43-45, 55-50, 60-66, 82, 89, and 140 kDa. The great diagnostic parameters reached by DEAE S2 suggest the potential applicability of this fraction in NC immunodiagnosis.
Departamento de Imunologia, Microbiologia e Parasitologia Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Avenida Pará 1720, CEP 38400-902, Uberlândia, Minas Gerais, Brazil.
Vanessa da S
Vda S
Ribeiro
Daniela da S
Dda S
Nunes
Henrique T
HT
Gonzaga
Jair P
JP
da Cunha-Junior
Julia M
JM
Costa-Cruz
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth / Q000276:immunology / Q000302:isolation & purification*; D005591:Chemical Fractionation; D002852:Chromatography, Ion Exchange; D004797:Enzyme-Linked Immunosorbent Assay; D004983:Ethanolamines / Q000737:chemistry*; D006801:Humans; D015151:Immunoblotting; D007074:Immunoglobulin G / Q000097:blood*; D020019:Neurocysticercosis / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D012680:Sensitivity and Specificity; D012698:Serologic Tests; D013552:Swine; D041201:Taenia solium / Q000276:immunology* / Q000302:isolation & purification
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D004983:Ethanolamines; D007074:Immunoglobulin G; C007369:2-diethylaminoethanol