Oleaginous fungal lipid fermentation on combined acid- and alkali-pretreated corn stover hydrolysate for advanced biofuel production.
A combined hydrolysis process, which first mixed dilute acid- and alkali-pretreated corn stover at a 1:1 (w/w) ratio, directly followed by enzymatic saccharification without pH adjustment, has been developed in this study in order to minimize the need of neutralization, detoxification, and washing during the process of lignocellulosic biofuel production. The oleaginous fungus Mortierella isabellina was selected and applied to the combined hydrolysate as well as a synthetic medium to compare fungal lipid accumulation and biodiesel production in both shake flask and 7.5L fermentor. Fungal cultivation on combined hydrolysate exhibited comparable cell mass and lipid yield with those from synthetic medium, indicating that the integration of combined hydrolysis with oleaginous fungal lipid fermentation has great potential to improve performance of advanced lignocellulosic biofuel production.
Department of Biosystems and Agricultural Engineering, Michigan State University, East Lansing, MI 48824, USA.
Zhenhua
Z
Ruan
Department of Biosystems and Agricultural Engineering, Michigan State University, East Lansing, MI 48824, USA.
Michael
M
Zanotti
Department of Biosystems and Agricultural Engineering, Michigan State University, East Lansing, MI 48824, USA.
Steven
S
Archer
Department of Biosystems and Agricultural Engineering, Michigan State University, East Lansing, MI 48824, USA.
Wei
W
Liao
Department of Biosystems and Agricultural Engineering, Michigan State University, East Lansing, MI 48824, USA. Electronic address: liuyan6@msu.edu.
Yan
Y
Liu
D000143:Acids / Q000737:chemistry*; D000468:Alkalies / Q000737:chemistry*; D056804:Biofuels; D005285:Fermentation; D006868:Hydrolysis; D008055:Lipids / Q000737:chemistry*; D020094:Mortierella / Q000378:metabolism; D003313:Zea mays / Q000378:metabolism*
D000143:Acids; D000468:Alkalies; D056804:Biofuels; D008055:Lipids
Biodiesel; Combined hydrolysis; Lignocellulosic biomass; Lipid accumulation; Oleaginous fungus