Rapid detection of infectious flacherie virus of the silkworm, Bombyx mori, using RT-PCR and nested PCR.
In this study, a method for detection of an ssRNA viral pathogen that causes viral flacherie in the silkworm, Bombyx mori (L.) (Lepidoptera: Bombycidae), was used for the detection of B. mori infectious flacherie virus (BmIFV). A combination of nested and reverse transcriptase polymerase chain reaction was used for detection. Although BmIFV has been reported in almost all the sericultural regions of the world, there had been no reports of BmIFV incidence in India. Therefore, the confirmation of the presence of BmIFV in Karnataka, India, is of great significance. The present method is advantageous because it can be used to detect the virus by using samples from infected midgut tissues, thus simplifying and avoiding laborious genome isolation procedures. This method could help in early detection of BmIFV disease pathogens and help reduce crop losses.
1 Post Graduate Department of Biotechnology and Microbiology, Karnatak University, Dharwad, India.
Shyam Kumar
SK
Vootla
Xing Meng
XM
Lu
Neetha
N
Kari
Mallikarjun
M
Gadwala
Qineng
Q
Lu
D000818:Animals; D012831:Bombyx / Q000235:genetics / Q000254:growth & development / Q000821:virology*; D007814:Larva / Q000235:genetics / Q000821:virology; D016133:Polymerase Chain Reaction; D012328:RNA Viruses / Q000235:genetics / Q000302:isolation & purification*; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D012367:RNA, Viral