Prevalence of helicobacter in laboratory mice in Thailand.
Prevalence of Helicobacter is mostly unknown in laboratory animals in Thailand. The 221 mice feces/cecum from 8 universities, 2 pharmaceutical companies and 3 research institutions in Thailand were surveyed for the prevalence and distribution of Helicobacter species by using the Electrochemical DNA chip. Helicobacter were detected 23/46 samples in Specific Pathogen Free (SPF) and 168/175 in conventional condition. Prevalence of Helicobacter were 98%, 96%, 92% and 78% in South (n=40), Northeast (n=40), North (n=25) and Central area (n=116), respectively. Only Central area holds SPF facility resulting in Helicobacter prevalence that seems to be lower than other areas. Three species of Helicobacter were detected in feces/cecum samples by sequence analysis: H. rodentium (67.0%, 148 samples), Helicobacter sp. MIT 01-6451 (15.4%, 34 samples), and unidentified Helicobacter species (14.1%, 9 samples). The results suggested that H. rodentium is the most common species of Helicobacter in laboratory mice in Thailand.
National Laboratory Center, Mahidol University, Salaya, Buddhamonthon, Nakhonprathom 73170, Thailand.
Mathurot
M
Duangchanchot
Rapee
R
Inpunkaew
Pravate
P
Thongsiri
Nobuhito
N
Hayashimoto
Nobuhiro
N
Gemma
Masaru
M
Nikaido
Masayoshi
M
Takahashi
Kanchana
K
Kengkoom
D000818:Animals; D000830:Animals, Laboratory / Q000382:microbiology*; D002432:Cecum / Q000382:microbiology; D005243:Feces / Q000382:microbiology; D016998:Helicobacter / Q000302:isolation & purification* / Q000472:pathogenicity; D007753:Laboratories / Q000706:statistics & numerical data*; D051379:Mice / Q000382:microbiology; D020411:Oligonucleotide Array Sequence Analysis; D015995:Prevalence; D013047:Specific Pathogen-Free Organisms; D013785:Thailand / Q000453:epidemiology