Photothermal effect for localized desorption of primary lymphocytes arrayed on an antibody/DNA-based biochip.
This work proposes a miniaturized system able to perform multiple cell capture followed by cell-type selective release from a biochip surface. Unlabelled lymphocytes were first specifically captured onto a DNA array by antibody-DNA conjugates. The immobilized cells were subsequently released under spatiotemporal control within local heating generated by intense Surface Plasmon Resonance (SPR) produced by laser illumination.
Univ. Grenoble Alpes, CNRS and CEA, INAC-SPRAM, F-38000 Grenoble, France. loic.leroy@cea.fr.
Loïc
L
Leroy
Radoslaw
R
Bombera
Elodie
E
Engel
Roberto
R
Calemczuk
Loïc
L
Laplatine
Dieu-donné R
DD
Baganizi
Patrice N
PN
Marche
Yoann
Y
Roupioz
Thierry
T
Livache
D000818:Animals; D000906:Antibodies / Q000737:chemistry*; D001402:B-Lymphocytes / Q000737:chemistry* / Q000378:metabolism; D018914:Cells, Immobilized / Q000166:cytology / Q000378:metabolism; D004247:DNA / Q000737:chemistry*; D051379:Mice; D020349:Surface Plasmon Resonance / Q000295:instrumentation / Q000379:methods; D013601:T-Lymphocytes / Q000166:cytology* / Q000378:metabolism; D046888:Tissue Array Analysis / Q000295:instrumentation / Q000379:methods
D000906:Antibodies; D004247:DNA