Oxidative stress and cellular immunity in patients with recurrent aphthous ulcers.
Recurrent aphthous ulcer (RAU) is an inflammatory condition of the oral mucosa characterized by painful, well-circumscribed, single or multiple round or ovoid ulcerations. The exact etiologic factor(s) of these ulcerations are not yet understood. The objective of this study was to evaluate inflammatory processes and free radical metabolism of 25 patients with RAUs compared to 25 healthy controls. The levels of malondialdehyde (MDA) and glutathione (GSH) were determined by high-performance liquid chromatography. Tumor necrosis factor-alpha (TNF-α), interleukin-2 (IL-2), IL-10, and IL-12 were determined by ELISA. Nitric oxide (NO), myeloperoxidase (MPO), total antioxidant status (TAS), and total oxidant status (TOS) levels were measured spectroscopically in serum. The levels of MDA, GSH, TNF-α, IL-2, IL-12, MPO, and TOS, and oxidative stress index (OSI) were higher, and the levels of NO, IL-10, and TAS were lower in patients with RAU than in controls. Statistical analysis showed that GSH, TNF-α, IL-2, IL-10, and OSI differed significantly in patients with RAU compared to controls. These parameters have important roles in oxidant/antioxidant defense.
Department of Molecular Biology and Genetics/Biochemistry, Faculty of Science and Arts, Hitit University, Corum, Turkey.
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Avci
Department of Dentomaxillofacial Radiology, Faculty of Dentistry, Gazi University, Ankara, Turkey.
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Akarslan
Department of Operative Dentistry, Faculty of Dentistry, Gazi University, Ankara, Turkey.
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Erten
Department of Biology, Faculty of Science and Arts, Gazi University, Ankara, Turkey.
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Coskun-Cevher
D000328:Adult; D000975:Antioxidants / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005609:Free Radicals / Q000378:metabolism; D005978:Glutathione / Q000097:blood*; D006801:Humans; D007111:Immunity, Cellular / Q000276:immunology*; D016753:Interleukin-10 / Q000097:blood; D018664:Interleukin-12 / Q000097:blood; D007376:Interleukin-2 / Q000097:blood; D008297:Male; D008315:Malondialdehyde / Q000097:blood*; D009569:Nitric Oxide / Q000097:blood; D018384:Oxidative Stress / Q000276:immunology* / Q000502:physiology; D009195:Peroxidase / Q000097:blood; D013281:Stomatitis, Aphthous / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
D000975:Antioxidants; D005609:Free Radicals; D007376:Interleukin-2; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D018664:Interleukin-12; D009569:Nitric Oxide; D008315:Malondialdehyde; D009195:Peroxidase; D005978:Glutathione