TNF-α, IL-6, and leptin increase the expression of miR-378, an adipogenesis-related microRNA in human adipocytes.
Obesity has become a global public health problem associated with complications including type 2 diabetes, cardiovascular disease, and several cancers. Adipocyte differentiation (adipogenesis) plays an important role in obesity and energy homeostasis. Adipose tissue secretes multiple cytokines and adipokines which can cause the complications of obesity, especially insulin resistance. TNF-α, IL-6, leptin, and resistin have been identified as the main regulators of obesity and insulin activity. miR-378 is highly induced during adipogenesis and has been reported to be positively regulated in adipogenesis. In the current study, matured human adipocytes were treated with TNF-α, IL-6, leptin, or resistin on the 15th day after the induction of human pre-adipocyte differentiation. We demonstrated that TNF-α, IL-6, and leptin upregulated miR-378 expression indicating that miR-378 probably is a novel mediator in the development of insulin resistance related to obesity.
Department of Pediatrics, Affiliated Hospital of Nantong University, Nantong, 226000, People's Republic of China.
Lu-lian
LL
Xu
Chun-mei
CM
Shi
Guang-feng
GF
Xu
Ling
L
Chen
Ling-ling
LL
Zhu
Lu
L
Zhu
Xi-rong
XR
Guo
Mei-yu
MY
Xu
Chen-bo
CB
Ji
D017667:Adipocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D050156:Adipogenesis / Q000187:drug effects*; D006801:Humans; D015850:Interleukin-6 / Q000494:pharmacology*; D020738:Leptin / Q000494:pharmacology*; D035683:MicroRNAs / Q000235:genetics*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*; D015854:Up-Regulation / Q000187:drug effects*
D015850:Interleukin-6; D020738:Leptin; C526012:MIRN378 microRNA, human; D035683:MicroRNAs; D014409:Tumor Necrosis Factor-alpha