Structure-related antibacterial activity of a titanium nanostructured surface fabricated by glancing angle sputter deposition.
The aim of this study was to reproduce the physico-mechanical antibacterial effect of the nanocolumnar cicada wing surface for metallic biomaterials by fabrication of titanium (Ti) nanocolumnar surfaces using glancing angle sputter deposition (GLAD). Nanocolumnar Ti thin films were fabricated by GLAD on silicon substrates. S. aureus as well as E. coli were incubated with nanostructured or reference dense Ti thin film test samples for one or three hours at 37 °C. Bacterial adherence, morphology, and viability were analyzed by fluorescence staining and scanning electron microscopy and compared to human mesenchymal stem cells (hMSCs).Bacterial adherence was not significantly different after short (1 h) incubation on the dense or the nanostructured Ti surface. In contrast to S. aureus the viability of E. coli was significantly decreased after 3 h on the nanostructured film compared to the dense film and was accompanied by an irregular morphology and a cell wall deformation. Cell adherence, spreading and viability of hMSCs were not altered on the nanostructured surface. The results show that the selective antibacterial effect of the cicada wing could be transferred to a nanostructured metallic biomaterial by mimicking the natural nanocolumnar topography.
Surgical Research, Department of Surgery, BG University Hospital Bergmannsheil, Ruhr-University Bochum, Germany.
Christina
C
Sengstock
Michael
M
Lopian
Yahya
Y
Motemani
Anna
A
Borgmann
Chinmay
C
Khare
Pio John S
PJ
Buenconsejo
Thomas A
TA
Schildhauer
Alfred
A
Ludwig
Manfred
M
Köller
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001422:Bacterial Adhesion / Q000187:drug effects; D004926:Escherichia coli / Q000166:cytology / Q000187:drug effects; D006430:Hemiptera; D006801:Humans; D050296:Microbial Viability / Q000187:drug effects; D049329:Nanostructures / Q000648:ultrastructure; D013211:Staphylococcus aureus / Q000166:cytology / Q000187:drug effects; D013499:Surface Properties; D014025:Titanium / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D014025:Titanium