Proteomics meets blue biotechnology: a wealth of novelties and opportunities.
Blue biotechnology, in which aquatic environments provide the inspiration for various products such as food additives, aquaculture, biosensors, green chemistry, bioenergy, and pharmaceuticals, holds enormous promise. Large-scale efforts to sequence aquatic genomes and metagenomes, as well as campaigns to isolate new organisms and culture-based screenings, are helping to push the boundaries of known organisms. Mass spectrometry-based proteomics can complement 16S gene sequencing in the effort to discover new organisms of potential relevance to blue biotechnology by facilitating the rapid screening of microbial isolates and by providing in depth profiles of the proteomes and metaproteomes of marine organisms, both model cultivable isolates and, more recently, exotic non-cultivable species and communities. Proteomics has already contributed to blue biotechnology by identifying aquatic proteins with potential applications to food fermentation, the textile industry, and biomedical drug development. In this review, we discuss historical developments in blue biotechnology, the current limitations to the known marine biosphere, and the ways in which mass spectrometry can expand that knowledge. We further speculate about directions that research in blue biotechnology will take given current and near-future technological advancements in mass spectrometry.
CEA, DSV, IBEB, Lab Biochim System Perturb, Bagnols-sur-Cèze, F-30207, France.
Erica M
EM
Hartmann
CEA, DSV, IBEB, Lab Biochim System Perturb, Bagnols-sur-Cèze, F-30207, France.
Emie
E
Durighello
CEA, DSV, IBEB, Lab Biochim System Perturb, Bagnols-sur-Cèze, F-30207, France.
Olivier
O
Pible
Microbiologia, Departament de Biologia, Universitat de les Illes Balears, Palma de Mallorca, Spain.
Balbina
B
Nogales
Actygea Srl, Via R. Lepetit 34, 21040 Gerenzano, Italy.
Fabrizio
F
Beltrametti
Microbiologia, Departament de Biologia, Universitat de les Illes Balears, Palma de Mallorca, Spain.
Rafael
R
Bosch
School of Life Sciences, University of Warwick, Gibbet Hill Road, Coventry CV47AL, United Kingdom.
Joseph A
JA
Christie-Oleza
CEA, DSV, IBEB, Lab Biochim System Perturb, Bagnols-sur-Cèze, F-30207, France. Electronic address: jean.armengaud@cea.fr.
Jean
J
Armengaud
D001688:Biological Products / Q000302:isolation & purification*; D001709:Biotechnology / Q000266:history / Q000379:methods* / Q000639:trends; D057166:High-Throughput Screening Assays / Q000379:methods* / Q000639:trends; D049674:History, 21st Century; D008386:Marine Biology / Q000266:history / Q000379:methods* / Q000639:trends; D013058:Mass Spectrometry / Q000379:methods; D040901:Proteomics / Q000379:methods* / Q000639:trends; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000379:methods
D001688:Biological Products; D012336:RNA, Ribosomal, 16S
High-throughput proteomics; Metaproteomics; Next-generation sequencing; Non-model organisms; Proteogenomics; Screening