The spatiotemporal expression and localization implicates a potential role for SerpinB11 in the process of mouse spermatogenesis and apoptosis.
In this study, the spatiotemporal expression of SerpinB11 in the mouse testis from postnatal 1-60 d was checked, the SerpinB11 protein strongly localized in the intermediate spermatogonia, B-type spermatogonium, preleptotene spermatocyte, leptonema spermatocyte, zygotene spermatocyte, but weakly localized in the pachytene spermatocyte, diplotene spermatocyte, sphere sperm, and the apoptotic sperm was positive stained of SerpinB11 protein, the localization of cell cycle marker CDK4 and meiosis marker SCP3 were investigated, and the SCP3 and SerpinB11 colocalized in the intermediate spermatogonia, B-type spermatogonium, preleptotene spermatocyte. Taken together, these results suggested that SerpinB11 might involved in spermatogenesis and apoptosis.
a Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, Key Laboratory of Reproduction and Genetics in Ningxia, Department of Histology and Embryology , Ningxia Medical University , Yinchuan , Ningxia , P.R. China.
Yanzhou
Y
Yang
Wenzhi
W
Ma
Huiming
H
Ma
Miao
M
Sun
Qing
Q
Chang
Xiuying
X
Pei
Yanrong
Y
Wang
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D018797:Cell Cycle Proteins; D051358:Cyclin-Dependent Kinase 4 / Q000378:metabolism; D004268:DNA-Binding Proteins; D008297:Male; D008813:Mice, Inbred ICR; D009687:Nuclear Proteins / Q000378:metabolism; D015843:Serpins / Q000378:metabolism*; D013091:Spermatogenesis / Q000502:physiology*; D013094:Spermatozoa / Q000378:metabolism; D013737:Testis / Q000378:metabolism*
D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; C523318:SERPINB11 protein, mouse; D015843:Serpins; C490062:Sycp3 protein, mouse; C495902:Cdk4 protein, mouse; D051358:Cyclin-Dependent Kinase 4
SCP3; SerpinB11; apoptosis; localization; spatiotemporal expression; spermatogenesis