A novel protein is lower expressed in renal cell carcinoma.
Engrailed-2 (EN2) has been identified as a candidate oncogene in breast cancer and prostate cancer. It is usually recognized as a mainly nuclear staining in the cells. However, recent studies showed a cytoplasmic staining occurred in prostate cancer, bladder cancer and clear cell renal cell carcinoma. The inconsistency makes us confused. To clarify the localization and expression of EN2 in renal cell carcinoma, anti-EN2 antibody (ab28731) and anti-EN2 antibody (MAB2600) were used for immunohistochemistry (IHC) respectively. Interestingly, we found that EN2 detected by ab28731 was mainly presented in cytoplasm while EN2 detected by MAB2600 was mainly presented in nucleus. To further investigate the different patterns observed above, lysates from full-length EN2 over expression in HEK293T cells were used to identify which antibody the EN2 molecule bound by western blot. Results showed ab28731 did not react with the lysates. For this reason, the novel specific protein detected by ab28731 was not the EN2 molecule and was named nonEN2. Then using the renal carcinoma tissue microarray and renal tissues, we found that the protein expression levels of nonEN2 in kidney tumor tissues was significantly lower than that in kidney normal tissues (p < 0.05), so was in renal cell lines. Taken together, nonEN2 is lower expressed and may play an important role in renal cell carcinoma.
Andrology Center, Peking University First Hospital, Peking University, Beijing 100034, China. guanruili@gmail.com.
Ruili
R
Guan
Andrology Center, Peking University First Hospital, Peking University, Beijing 100034, China. xyongde@gmail.com.
Yongde
Y
Xu
Andrology Center, Peking University First Hospital, Peking University, Beijing 100034, China. hongenlei@foxmail.com.
Hongen
H
Lei
Andrology Center, Peking University First Hospital, Peking University, Beijing 100034, China. gaozhezhu@gmail.com.
Zhezhu
Z
Gao
Andrology Center, Peking University First Hospital, Peking University, Beijing 100034, China. xinzc@bjmu.edu.cn.
Zhongcheng
Z
Xin
Department of Urology, Peking University First Hospital and the Institute of Urology, Peking University, Beijing 100034, China. guoyinglu07@gmail.com.
Yinglu
Y
Guo
D000595:Amino Acid Sequence; D014408:Biomarkers, Tumor / Q000032:analysis* / Q000235:genetics; D002292:Carcinoma, Renal Cell / Q000235:genetics / Q000473:pathology*; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D057809:HEK293 Cells; D018398:Homeodomain Proteins / Q000032:analysis* / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry; D007668:Kidney / Q000378:metabolism / Q000473:pathology*; D007680:Kidney Neoplasms / Q000235:genetics / Q000473:pathology*; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000032:analysis* / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000032:analysis* / Q000235:genetics; D000099266:Engrailed 2 Protein
D014408:Biomarkers, Tumor; D018398:Homeodomain Proteins; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D000099266:Engrailed 2 Protein; C000594528:nonEN2 protein, human