Heterologous expression of triterpene biosynthetic genes in yeast and subsequent metabolite identification through GC-MS.
Heterologous expression of plant metabolic enzymes in microorganisms is extensively used for identifying genes involved in their pathways and producing useful compounds. Here, we describe a plasmid-based yeast expression system that easily allows the expression of different triterpene biosynthetic genes in wild-type yeast, providing a useful platform for identifying their functions and facilitating combinatorial biosynthesis of triterpenoids.
Frontier Research Base for Global Young Researchers, Graduate School of Engineering, Osaka University, Osaka, Japan.
Ery Odette
EO
Fukushima
Hikaru
H
Seki
Toshiya
T
Muranaka
D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D015870:Gene Expression; D005800:Genes, Fungal / Q000235:genetics*; D005818:Genetic Engineering / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014170:Transformation, Genetic; D014315:Triterpenes / Q000378:metabolism*
D014315:Triterpenes