A generalized free-solvent model for the osmotic pressure of multi-component solutions containing protein-protein interactions.
The free-solvent model has been shown to have excellent predictability of the osmotic pressure for single and binary non-interactive proteins in aqueous solutions. Here the free-solvent model is extended to be more generalized by including the contributions of intra- and inter-protein interactions to the osmotic pressure of a solution in the form of homo- and hetero-multimers. The solute-solvent interactions are considered to be unique for each homo- and hetero-multimer in solution. The effect of the various generalized free-solvent model parameters on the osmotic pressure are examined for a single protein solution with a homo-dimer, a binary protein solution with no protein-protein interactions, and a binary protein solution with a hetero-dimer. Finally, the limitations associated with the generalized free-solvent model are discussed.
B2K Group (Biotransport & Bioreaction Kinetics Group), Center for Bioengineering Research, Department of Bioengineering, University of California, Riverside, Riverside, CA 92521, United States.
Devin W
DW
McBride
B2K Group (Biotransport & Bioreaction Kinetics Group), Center for Bioengineering Research, Department of Bioengineering, University of California, Riverside, Riverside, CA 92521, United States. Electronic address: vrodgers@engr.ucr.edu.
V G J
VG
Rodgers
D055598:Chemical Phenomena; D055641:Mathematical Concepts; D008956:Models, Chemical; D009997:Osmotic Pressure; D054730:Protein Interaction Domains and Motifs; D055503:Protein Multimerization; D011506:Proteins / Q000737:chemistry; D012996:Solutions; D012997:Solvents
D011506:Proteins; D012996:Solutions; D012997:Solvents
Crowded protein; Free-solvent model; Hydration; Ion binding; Multi-component; Osmotic pressure