The influence of the extent of enzymatic hydrolysis on antioxidative properties and ACE-inhibitory activities of protein hydrolysates from goby (Zosterisessor ophiocephalus) muscle.
Antioxidant properties and angiotensin-converting enzyme (ACE) inhibitory activities of protein hydrolysates from goby (Zosterisessor ophiocephalus) muscle, with different degrees of hydrolysis (DH) from 5 to 25%, prepared by treatment with crude proteases extract from smooth hound intestines, were investigated. Goby protein hydrolysates (GPHs) are rich in Gly and Thr, which accounted for 14.1-15% and 11.6-13.2% of the total amino acids, respectively. The antioxidant activities of GPHs were investigated by using several in vitro assay systems. All GPHs exhibited significant metal chelating activity and DPPH free radical-scavenging activity, and inhibited linoleic acid peroxidation. For the ACE-inhibitory activity, as the DH increased, the activity of GPHs increased. The obtained results revealed that antioxidant and ACE-inhibitory activities of GPHs were influenced by the degree of hydrolysis. A medium degree of enzymatic hydrolysis was appropriate to obtain GPHs with good antioxidant activity, while small peptides were essential to obtain high ACE inhibitory activity.
Laboratory of Microbiology and Enzyme Engineering, Sfax University, National School of Engineering, B.P 1173-3038, Sfax, Tunisia, ryma47@hotmail.fr.
Rim
R
Nasri
Mourad
M
Jridi
Imen
I
Lassoued
Ines
I
Jemil
Rabeb
R
Ben Slama-Ben Salem
Moncef
M
Nasri
Maha
M
Karra-Châabouni
D000806:Angiotensin-Converting Enzyme Inhibitors / Q000378:metabolism* / Q000494:pharmacology*; D000818:Animals; D001713:Biphenyl Compounds / Q000378:metabolism; D029941:Fish Proteins / Q000378:metabolism* / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000378:metabolism / Q000494:pharmacology; D006868:Hydrolysis; D007501:Iron / Q000378:metabolism; D009132:Muscles / Q000378:metabolism*; D010447:Peptide Hydrolases / Q000378:metabolism*; D010473:Perciformes; D010851:Picrates / Q000378:metabolism; D012754:Sharks; D019207:beta Carotene / Q000378:metabolism
D000806:Angiotensin-Converting Enzyme Inhibitors; D001713:Biphenyl Compounds; D029941:Fish Proteins; D016166:Free Radical Scavengers; D010851:Picrates; D019207:beta Carotene; C004931:1,1-diphenyl-2-picrylhydrazyl; D007501:Iron; D010447:Peptide Hydrolases