Hand contamination with human rhinovirus in Bangladesh.
As one step in developing a measure of hand contamination with respiratory viruses, this study assessed if human rhinovirus (HRV) was detectable on hands in a low income non-temperate community where respiratory disease is a leading cause of child death. Research assistants observed residents in a low income community in Dhaka, Bangladesh. When they observed a resident sneeze or pick their nose, they collected a hand rinse and anterior nare sample from the resident. Samples were first tested for HRV RNA by real-time RT-PCR (rRT-PCR). A subset of rRT-PCR positive samples were cultured into MRC-5 and HeLa Ohio cells. Among 177 hand samples tested for HRV by real-time RT-PCR, 52 (29%) were positive. Among 15 RT-PCR positive hand samples that were cultured, two grew HRV. HRV was detected in each of the sampling months (January, February, June, July, November, and December). This study demonstrates in the natural setting that, at least after sneezing or nasal cleaning, hands were contaminated commonly with potentially infectious HRV. Future research could explore if HRV RNA is present consistently and is associated sufficiently with the incidence of respiratory illness in communities that it may provide a proxy measure of respiratory viral hand contamination.
Centre for Communicable Diseases, International Centre for Diarrhoeal Disease Research, Dhaka, Bangladesh; Centers for Disease Control and Prevention, Atlanta, Georgia.
Stephen P
SP
Luby
Xiaoyan
X
Lu
Theresa
T
Cromeans
M A Yushuf
MA
Sharker
Mohammad Abdul
MA
Kadir
Dean D
DD
Erdman
D000293:Adolescent; D000328:Adult; D001459:Bangladesh; D002460:Cell Line; D002648:Child; D002675:Child, Preschool; D005260:Female; D006225:Hand / Q000821:virology*; D006801:Humans; D007223:Infant; D008297:Male; D009666:Nose / Q000821:virology; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012229:Rhinovirus / Q000302:isolation & purification*; D014776:Virus Cultivation; D055815:Young Adult
D012367:RNA, Viral
Bangladesh; hand; handwashing; rhinovirus