[Serotypic distribution of Chlamydia trachomatis infection of genital tract in males].
OBJECTIVE
To explore the serotypic distribution and ompA gene variations of Chlamydia trachomatis (C.trachomatis) of male reproductive tract at our hospital.


METHODS
A total of 399 samples of reproductive tract secretion were collected from urinary outpatient with suspected C.trachomatis infection of genital tract from December 2011 to March 2013 and screened by C.trachomatis detection kit. Amplificated by nested PCR and sequenced of C.trachomatis ompA gene after screening positive samples.Sequencing products were typed by BLAST of PubMed and MegAlign program of DNAstar software to compare the clinical isolates of C.trachomatis ompA gene with the standard sequence. And various serotypes of C.trachomatis ompA gene mutation were analyzed.


RESULTS
C.trachomatis was detected with a positive rate of 14.8% (59/399). All ompA genes of 59 C.trachomatis positive specimens were successfully amplified and serotyped. The types were E (n = 17, 28.8%), F (n = 16, 27.1%), D (n = 13, 22.0%),G (n = 5, 8.5%), H (n = 3, 5.1%), K (n = 3, 5.1%) and J (n = 2, 3.4%). The most mutations of ompA gene of C.trachomatis in clinical specimens were serotype D (n = 9) , followed by serotype G(n = 5).


CONCLUSIONS
The distribution of serotypes in male patients is serotypes E, F, D-type dominant. The serotype D of C.trachomatis is variation up.
Beijing Tongren Hospital Affiliated to Capital Medical University, Beijing 100730, China.
Yufeng
Y
Sun
Beijing Tongren Hospital Affiliated to Capital Medical University, Beijing 100730, China.
Xi
X
Yang
Beijing Tongren Hospital Affiliated to Capital Medical University, Beijing 100730, China.
Yuexin
Y
Liu
Beijing Tongren Hospital Affiliated to Capital Medical University, Beijing 100730, China.
Lijun
L
Wang
Beijing Tongren Hospital Affiliated to Capital Medical University, Beijing 100730, China.
Gui
G
Zhang
Beijing Tongren Hospital Affiliated to Capital Medical University, Beijing 100730, China. Email: luxinxin2009@126.com.
Xinxin
X
Lu
D000328:Adult; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D002690:Chlamydia Infections / Q000382:microbiology*; D002692:Chlamydia trachomatis / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D005838:Genotype; D006801:Humans; D008297:Male; D052801:Male Urogenital Diseases / Q000382:microbiology*; D009154:Mutation; D017422:Sequence Analysis, DNA; D012703:Serotyping
D001425:Bacterial Outer Membrane Proteins; C078167:OMPA outer membrane proteins