Skin barrier disruptions in tape stripped and allergic dermatitis models have no effect on dermal penetration and systemic distribution of AHAPS-functionalized silica nanoparticles.
The skin is a potential site of entry for nanoparticles (NP) but the role of disease-associated barrier disturbances on the path and extent of skin penetration of NP remains to be characterized. Silica nanoparticles (SiO2-NP) possess promising potential for various medical applications. Here, effects of different skin barrier disruptions on the penetration of N-(6-aminohexyl)-aminopropyltrimethoxysilane (AHAPS) functionalized SiO2-NP were studied. AHAPS-SiO2-NP (55±6 nm diameter) were topically applied on intact, tape stripped or on inflamed skin of SKH1 mice with induced allergic contact dermatitis for one or five consecutive days, respectively. Penetration of AHAPS-SiO2-NP through the skin was not observed regardless of the kind of barrier disruption. However, only after subcutaneous injection, AHAPS-SiO2-NP were incorporated by macrophages and transported to the regional lymph node only. Adverse effects on cells or tissues were not observed. In conclusion, AHAPS-SiO2-NP seem to not cross the normal or perturbed mouse skin. From the clinical editor: Skin is a potential site of entry for nanoparticles; however, it is poorly understood how skin diseases may alter this process. In tape-stripped skin and allergic contact dermatitis models the delivery properties of AHAPS-SiO2 nanoparticles remained unchanged, and in neither case were these NP-s able to penetrate the skin. No adverse effects were noted on the skin in these models and control mice.
Institute of Veterinary Pathology, Freie Universität Berlin, Berlin, Germany.
Anja
A
Ostrowski
Institute of Chemistry and Biochemistry-Physical and Theoretical Chemistry, Freie Universität Berlin, Berlin, Germany.
Daniel
D
Nordmeyer
Department of Physics, Institute of Experimental Physics, Freie Universität Berlin, Berlin, Germany.
Alexander
A
Boreham
Department of Physics, Institute of Experimental Physics, Freie Universität Berlin, Berlin, Germany; Helmholtz Virtual Institute-Multifunctional Biomaterials for Medicine, Helmholtz-Zentrum Geesthacht, Teltow, Germany.
Robert
R
Brodwolf
Institute of Veterinary Pathology, Freie Universität Berlin, Berlin, Germany.
Lars
L
Mundhenk
Department of Dermatology, Venerology and Allergology, Charité-Universitätsmedizin Berlin, Berlin, Germany.
Joachim W
JW
Fluhr
Department of Dermatology, Venerology and Allergology, Charité-Universitätsmedizin Berlin, Berlin, Germany.
Jürgen
J
Lademann
Institute of Chemistry and Biochemistry-Physical and Theoretical Chemistry, Freie Universität Berlin, Berlin, Germany.
Christina
C
Graf
Institute of Chemistry and Biochemistry-Physical and Theoretical Chemistry, Freie Universität Berlin, Berlin, Germany.
Eckart
E
Rühl
Department of Physics, Institute of Experimental Physics, Freie Universität Berlin, Berlin, Germany; Helmholtz Virtual Institute-Multifunctional Biomaterials for Medicine, Helmholtz-Zentrum Geesthacht, Teltow, Germany.
Ulrike
U
Alexiev
Institute of Veterinary Pathology, Freie Universität Berlin, Berlin, Germany. Electronic address: achim.gruber@fu-berlin.de.
Achim D
AD
Gruber
D000818:Animals; D003877:Dermatitis, Contact / Q000503:physiopathology*; D004195:Disease Models, Animal; D008297:Male; D051379:Mice; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles; D012822:Silicon Dioxide; D012867:Skin / Q000503:physiopathology*; D012869:Skin Absorption
D012822:Silicon Dioxide
Allergic contact dermatitis; FLIM; Fluorescence microscopy; Murine model; Tape stripping