The polysaccharides from porphyra yezoensis suppress the denaturation of bighead carp myofibrillar protein.
In this study, investigated was the effect of the porphyra yezoensis polysaccharides (PPs) on the denaturation of bighead carp (Aristichthys nobilis) myofibrillar protein (Mf) during frozen storage at -18°C for 90d. The PPs (2.5%, 5%, and 7.5%, respectively) was added to 100g of Mf. The changes in the Ca-adenylpyrophosphatase (ATPase) activity and unfrozen water content in Mf were examined to evaluate he denaturation of Mf during frozen storage. Ca-ATPase activity decreased gradually during frozen storage at -18°C upon addition of PPs. By contrast, Ca-ATPase activity in the control group dropped drastically during the first 45d of storage and then further decreased gradually for up to 90d of storage, indicating a biphasic denaturation pattern. PPs addition significantly increased sulfhydryl contents in the Mf of the treatment groups compared with that of the control group (p<0.05) during frozen storage at -18°C.
School of Marine Science and Technology, Huaihai Institute of Technology, 59 Cangwu Road, Xinpu 222005, China. Electronic address: jianglongfa008@sina.com.
Long-Fa
LF
Jiang
D000818:Animals; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D002347:Carps / Q000378:metabolism*; D015925:Cryopreservation; D029941:Fish Proteins / Q000737:chemistry* / Q000378:metabolism*; D011134:Polysaccharides / Q000494:pharmacology*; D044664:Porphyra / Q000737:chemistry*; D011489:Protein Denaturation / Q000187:drug effects*; D013438:Sulfhydryl Compounds / Q000378:metabolism
D029941:Fish Proteins; D011134:Polysaccharides; D013438:Sulfhydryl Compounds; D000252:Calcium-Transporting ATPases
Ca-ATPase; Myofibrillar protein; Sulfhydryl content