A colloidal gold nanoparticle-based immunochromatographic test strip for rapid and convenient detection of Staphylococcus aureus.
An immunochromatographic test strip using gold nanoparticles-staphylococcus aureus monoclonal antibody conjugates was developed for the rapid and convenient detection of staphylococcus aureus based on a double-antibody sandwich format. The detection limit and the detection rate of this test strip is 10(3) CFU /mL and 98.7%, respectively. It could be used for the rapid detection of staphylococcus aureus in food and the results can be visually identified by the naked eye within 10 min. Compared with conventional bacterial detection methods, this developed immunochromatographic assay based test strip has several advantages including simple, fast, low-cost, favorable sensitivity and specificity, exhibiting a great potential for application in food safety control systems and clinical diagnosis.
Kaili
K
Niu
Xiaoping
X
Zheng
Chusen
C
Huang
Kuan
K
Xul
Yuan
Y
Zhi
Hebai
H
Shen
Nengqin
N
Jia
D002846:Chromatography, Affinity / Q000295:instrumentation*; D003102:Colloids / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000295:instrumentation*; D010316:Particle Size; D011934:Reagent Strips; D013211:Staphylococcus aureus / Q000302:isolation & purification*
D003102:Colloids; D011934:Reagent Strips; D006046:Gold