Genetic profiling by single-nucleotide polymorphism-based array analysis defines three distinct subtypes of orbital meningioma.
Orbital meningiomas can be classified as primary optic nerve sheath (ON) meningiomas, primary intraorbital ectopic (Ob) meningiomas and spheno-orbital (Sph-Ob) meningiomas based on anatomic site. Single-nucleotide polymorphism (SNP)-based array analysis with the Illumina 300K platform was performed on formalin-fixed, paraffin-embedded tissue from 19 orbital meningiomas (5 ON, 4 Ob and 10 Sph-Ob meningiomas). Tumors were World Health Organization (WHO) grade I except for two grade II meningiomas, and one was NF2-associated. We found genomic alterations in 68% (13 of 19) of orbital meningiomas. Sph-Ob tumors frequently exhibited monosomy 22/22q loss (70%; 7/10) and deletion of chromosome 1p, 6q and 19p (50% each; 5/10). Among genetic alterations, loss of chromosome 1p and 6q were more frequent in clinically progressive tumors. Chromosome 22q loss also was detected in the majority of Ob meningiomas (75%; 3/4) but was infrequent in ON meningiomas (20%; 1/5). In general, Ob tumors had fewer chromosome alterations than Sph-Ob and ON tumors. Unlike Sph-Ob meningiomas, most of the Ob and ON meningiomas did not progress even after incomplete excision, although follow-up was limited in some cases. Our study suggests that ON, Ob and Sph-Ob meningiomas are three molecularly distinct entities. Our results also suggest that molecular subclassification may have prognostic implications.
Department of Pathology, Johns Hopkins University, Baltimore, MD; Division of Pathology, Children's National Medical Center, Washington, DC.
Cheng-Ying
CY
Ho
Stacy
S
Mosier
Janice
J
Safneck
Diva R
DR
Salomao
Neil R
NR
Miller
Charles G
CG
Eberhart
Christopher D
CD
Gocke
Denise A S
DA
Batista
Fausto J
FJ
Rodriguez
D000328:Adult; D000368:Aged; D002872:Chromosome Deletion; D002878:Chromosomes, Human, Pair 1 / Q000235:genetics; D002888:Chromosomes, Human, Pair 19 / Q000235:genetics; D002892:Chromosomes, Human, Pair 22 / Q000235:genetics; D002896:Chromosomes, Human, Pair 6 / Q000235:genetics; D005260:Female; D006801:Humans; D008297:Male; D008579:Meningioma / Q000145:classification / Q000235:genetics*; D008875:Middle Aged; D019574:Optic Nerve Neoplasms / Q000145:classification / Q000235:genetics*; D009918:Orbital Neoplasms / Q000145:classification / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D055815:Young Adult
NF2; SNP array; chromosome 22; cytogenetics; optic nerve sheath; orbital meningioma