Matrix-assisted laser desorption ionization-time of flight mass spectrometry can accurately differentiate Aeromonas dhakensis from A. hydrophila, A. caviae, and A. veronii.
Among 217 Aeromonas isolates identified by sequencing analysis of their rpoB genes, the accuracy rates of identification of A. dhakensis, A. hydrophila, A. veronii, and A. caviae were 96.7%, 90.0%, 96.7%, and 100.0%, respectively, by the cluster analysis of spectra generated by matrix-assisted laser desorption ionization-time of flight mass spectrometry.
Graduate Institute of Clinical Medicine, National Cheng Kung University, College of Medicine, Tainan, Taiwan Department of Internal Medicine, National Cheng Kung University, College of Medicine and Hospital, Tainan, Taiwan.
Po-Lin
PL
Chen
Department of Laboratory Medicine, National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei, Taiwan Department and Graduate Institute of Clinical Laboratory Sciences and Medical Biotechnology, National Taiwan University, Taipei, Taiwan.
Tai-Fen
TF
Lee
Graduate Institute of Clinical Medicine, National Cheng Kung University, College of Medicine, Tainan, Taiwan Department of Internal Medicine, National Cheng Kung University, College of Medicine and Hospital, Tainan, Taiwan National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Tainan, Taiwan.
Chi-Jung
CJ
Wu
Bruker Taiwan Co., Ltd., Taipei, Taiwan.
Shih-Hua
SH
Teng
Department of Laboratory Medicine, National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei, Taiwan Department and Graduate Institute of Clinical Laboratory Sciences and Medical Biotechnology, National Taiwan University, Taipei, Taiwan.
Lee-Jene
LJ
Teng
Department of Internal Medicine, National Cheng Kung University, College of Medicine and Hospital, Tainan, Taiwan Center for Infection Control, National Cheng Kung University, College of Medicine and Hospital, Tainan, Taiwan winston3415@gmail.com hsporen@ntu.edu.tw.
Wen-Chien
WC
Ko
Department of Laboratory Medicine, National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei, Taiwan Department of Internal Medicine, National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei, Taiwan winston3415@gmail.com hsporen@ntu.edu.tw.
Po-Ren
PR
Hsueh
D000333:Aeromonas / Q000737:chemistry* / Q000145:classification*; D001431:Bacteriological Techniques / Q000379:methods*; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D006801:Humans; D017422:Sequence Analysis, DNA; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit