Comparison of adaptive optics scanning light ophthalmoscopic fluorescein angiography and offset pinhole imaging.
Recent advances to the adaptive optics scanning light ophthalmoscope (AOSLO) have enabled finer in vivo assessment of the human retinal microvasculature. AOSLO confocal reflectance imaging has been coupled with oral fluorescein angiography (FA), enabling simultaneous acquisition of structural and perfusion images. AOSLO offset pinhole (OP) imaging combined with motion contrast post-processing techniques, are able to create a similar set of structural and perfusion images without the use of exogenous contrast agent. In this study, we evaluate the similarities and differences of the structural and perfusion images obtained by either method, in healthy control subjects and in patients with retinal vasculopathy including hypertensive retinopathy, diabetic retinopathy, and retinal vein occlusion. Our results show that AOSLO OP motion contrast provides perfusion maps comparable to those obtained with AOSLO FA, while AOSLO OP reflectance images provide additional information such as vessel wall fine structure not as readily visible in AOSLO confocal reflectance images. AOSLO OP offers a non-invasive alternative to AOSLO FA without the need for any exogenous contrast agent.
Department of Ophthalmology, New York Eye & Ear Infirmary, New York, NY 10003, USA.
Toco Y P
TY
Chui
Department of Ophthalmology, New York Eye & Ear Infirmary, New York, NY 10003, USA ; Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.
Michael
M
Dubow
Department of Ophthalmology, New York Eye & Ear Infirmary, New York, NY 10003, USA ; Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.
Alexander
A
Pinhas
Department of Ophthalmology, New York Eye & Ear Infirmary, New York, NY 10003, USA.
Nishit
N
Shah
Department of Ophthalmology, New York Eye & Ear Infirmary, New York, NY 10003, USA.
Alexander
A
Gan
Department of Ophthalmology, New York Eye & Ear Infirmary, New York, NY 10003, USA.
Rishard
R
Weitz
The Institute of Optics, University of Rochester, Rochester, NY 14627, USA.
Yusufu N
YN
Sulai
Department of Biomedical Engineering, Marquette University, Milwaukee, WI 53233, USA ; Department of Ophthalmology, Medical College of Wisconsin, Milwaukee, WI 53226, USA ; Department of Biophysics, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
Alfredo
A
Dubra
Department of Ophthalmology, New York Eye & Ear Infirmary, New York, NY 10003, USA ; Department of Ophthalmology, New York Medical College, Valhalla, NY 10595, USA.
Richard B
RB
Rosen
(110.1080) Active or adaptive optics; (170.4460) Ophthalmic optics and devices; (170.4470) Ophthalmology; (290.4210) Multiple scattering