Laser polarization autofluorescence of endogenous porphyrins of optically anisotropic biological tissues and fluids in diagnostics of necrotic and pathological changes of human organs.
This research presents the results of investigation of laser polarization fluorescence of biological layers (histological sections, cytological smears). The polarization structural properties of autofluorescent images of human biological tissues layers and fluids were found and investigated. A model describing the formation of polarizationally heterogeneous images of optically anisotropic biological layers is suggested. On this basis, the practical method of polarization-variable autofluorescence is analytically substantiated and experimentally tested. The efficiency of applying this method to various tasks of medical diagnostics is analyzed: objectification of histological conclusions, defining and differentiating of various forms of cancer (dysplasia--microinvasive cancer) of the cervix uteri, and forensic medical express-differentiation of cause of death. The objective criteria (statistical moments) of differentiation of autofluorescent images of histological sections of myocardium biopsy and endometrium and cytological smears of its mucous tunic are defined. The operational characteristics (sensitivity, specificity, accuracy) of this method are determined concerning the positions of probative medicine, and the clinical efficiency of the technique is demonstrated.
Yu A
YA
Ushenko
A D
AD
Arkhelyuk
M I
MI
Sidor
V T
VT
Bachynskyi
O Yu
OY
Wanchuliak
D016880:Anisotropy; D014408:Biomarkers, Tumor / Q000032:analysis*; D005260:Female; D006801:Humans; D007834:Lasers; D008859:Microscopy, Polarization / Q000379:methods*; D057054:Molecular Imaging / Q000379:methods; D061848:Optical Imaging / Q000379:methods*; D011166:Porphyrins / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D002583:Uterine Cervical Neoplasms / Q000737:chemistry* / Q000175:diagnosis*
D014408:Biomarkers, Tumor; D011166:Porphyrins