Dependence on the CCR5 coreceptor for viral replication explains the lack of rebound of CXCR4-predicted HIV variants in the Berlin patient.
The "Berlin patient" is the first patient cured of HIV-1 infection after allogeneic transplantation with nonfunctional CCR5 coreceptor stem cells. We demonstrate that CXCR4-predicted minority viruses present prior to transplantation were unable to rebound after transplantation due to their dependence on CCR5 for replication and high genetic barrier toward CXCR4 usage.
Department of Medical Microbiology and Virology, University Medical Center, Utrecht, The Netherlands.
Jori
J
Symons
Department of Internal Medicine, Infectious Diseases and Psychosomatic Medicine, Ghent University Hospital, Belgium.
Linos
L
Vandekerckhove
Institute of Transfusion Medicine and Immunology, Heidelberg, Germany.
Gero
G
Hütter
Department of Medical Microbiology and Virology, University Medical Center, Utrecht, The Netherlands.
Annemarie M J
AM
Wensing
Department of Medical Microbiology and Virology, University Medical Center, Utrecht, The Netherlands.
Petra M
PM
van Ham
Department of Medicine, University of California, San Francisco.
Steven G
SG
Deeks
Department of Medical Microbiology and Virology, University Medical Center, Utrecht, The Netherlands.
Monique
M
Nijhuis
D015658:HIV Infections / Q000628:therapy / Q000821:virology*; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000150:complications / Q000628:therapy*; D019713:Receptors, CCR5 / Q000378:metabolism*; D019718:Receptors, CXCR4 / Q000378:metabolism; D015728:Receptors, HIV / Q000378:metabolism*; D033581:Stem Cell Transplantation; D016896:Treatment Outcome; D056189:Viral Tropism; D014779:Virus Replication
C584933:CCR5 protein, human; C527276:CXCR4 protein, human; D019713:Receptors, CCR5; D019718:Receptors, CXCR4; D015728:Receptors, HIV
Berlin-Patient; HIV; coreceptor; cure; stem cell transplantation