[Identification of radix et rhizoma clematidis and its adulterants using DNA barcoding].
This study provides the candidate sequences in the identification of Radix et Rhizoma Clematidis and its adulterants using DNA barcoding. We amplified and sequenced the region psbA-trnH, with the data of 284 sequences from GenBank, the differential intra- and inter-specific divergences, genetic distance, barcoding gap were used to evaluate five barcodes, and the identification efficiency was assessed using BLAST1 and Nearest Distance methods. The results showed that psbA-trnH barcodes performed high identification efficiency and inter-specific divergences among the five different DNA barcodes. Analysis of the barcoding gap and NJ tree showed psbA-trnH was superior to other barcodes. Based on the identification and PCR amplification efficiency, psbA-trnH can be the ideal barcode to identify Radix et Rhizoma Clematidis and its adulterants accurately.
Shan-Shan
SS
Feng
Si-Hao
SH
Zheng
Ya-Kang
YK
Li
Lin-Fang
LF
Huang
D058893:DNA Barcoding, Taxonomic / Q000379:methods*; D018744:DNA, Plant / Q000235:genetics*; D004340:Drug Contamination; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D018517:Plant Roots / Q000235:genetics; D010946:Plants, Medicinal / Q000145:classification / Q000235:genetics*; D029626:Ranunculaceae / Q000145:classification / Q000235:genetics*; D027343:Rhizome / Q000235:genetics; D013045:Species Specificity
D018744:DNA, Plant