Influence of infection by Toxoplasma gondii on purine levels and E-ADA activity in the brain of mice experimentally infected mice.
The aim of this study was to assess the purine levels and E-ADA activity in the brain of mice (BALB/c) experimentally infected with Toxoplasma gondii. In experiment I (n=24) the mice were infected with RH strain of T. gondii, while in experiment II (n=36) they were infected with strain ME-49 of T. gondii. Our results showed that, for RH strain (acute phase), an increase in both periods in the levels of ATP, ADP, AMP, adenosine, hypoxanthine, xanthine (only on day 6 PI) and uric acid (only on day 6 PI). By the other hand, the RH strain led, on days 4 and 6 PI, to a reduction in the concentration of inosine. ME-49, a cystogenic strain, showed some differences in acute and chronic phase, since on day 6 PI the levels of ATP and ADP were increased, while on day 30 these same nucleotides were reduced. On day 60 PI, ME-49 induced a reduction in the levels of ATP, ADP, AMP, adenosine, inosine and xanthine, while uric acid was increased. A decrease of E-ADA activity was observed in brain on days 4 and 6 PI (RH), and 30 PI (ME-49); however on day 60 PI E-ADA activity was increased for infection by ME-49 strain. Therefore, it was possible to conclude that infection with T. gondii changes the purine levels and the activity of E-ADA in brain, which may be associated with neurological signs commonly observed in this disease.
Department of Microbiology and Parasitology, Universidade Federal de Santa Maria, Brazil; Department of Small Animal, Universidade Federal de Santa Maria, Brazil. Electronic address: tonin_alexandre@yahoo.com.br.
Alexandre A
AA
Tonin
Department of Animal Science, Universidade do Estado de Santa Catarina, Brazil. Electronic address: aleksandro_ss@yahoo.com.br.
Aleksandro S
AS
Da Silva
Department of Morphological Science, Universidade Federal do Rio Grande do Sul, Brazil; Department of Biochemistry, Universidade Federal do Rio Grande do Sul, Brazil.
Emerson A
EA
Casali
Department of Morphological Science, Universidade Federal do Rio Grande do Sul, Brazil.
Stephanie S
SS
Silveira
Department of Morphological Science, Universidade Federal do Rio Grande do Sul, Brazil.
Cesar E J
CE
Moritz
Department of Preventive Veterinary Medicine, Universidade Federal de Santa Maria, Brazil.
Giovana
G
Camillo
Department of Veterinary Pathology, Universidade Federal de Santa Maria, Brazil.
Mariana M
MM
Flores
Department of Veterinary Pathology, Universidade Federal de Santa Maria, Brazil.
Rafael
R
Fighera
Department of Biochemistry, Universidade Federal do Rio Grande do Sul, Brazil.
Gustavo R
GR
Thomé
Department of Biochemistry, Universidade Federal do Rio Grande do Sul, Brazil.
Vera M
VM
Morsch
Department of Biochemistry, Universidade Federal do Rio Grande do Sul, Brazil.
Maria Rosa C
MR
Schetinger
Department of Microbiology and Parasitology, Universidade Federal de Santa Maria, Brazil; Department of Small Animal, Universidade Federal de Santa Maria, Brazil.
Mario De La
Mde L
Rue
Department of Preventive Veterinary Medicine, Universidade Federal de Santa Maria, Brazil.
Fernanda S F
FS
Vogel
Department of Microbiology and Parasitology, Universidade Federal de Santa Maria, Brazil; Department of Small Animal, Universidade Federal de Santa Maria, Brazil.
Sonia T A
ST
Lopes
D000243:Adenosine Deaminase / Q000032:analysis / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D002851:Chromatography, High Pressure Liquid; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011687:Purines / Q000032:analysis / Q000378:metabolism*; D013053:Spectrophotometry; D013997:Time Factors; D014122:Toxoplasma / Q000145:classification / Q000472:pathogenicity; D014124:Toxoplasmosis, Animal / Q000378:metabolism* / Q000473:pathology; D014774:Virulence
D011687:Purines; D000243:Adenosine Deaminase
Ectoenzymes; ME-49; Purinergic signaling; RH; Toxoplasmosis