A survey of aflatoxin in cotton seed in Iran by HPLC with on-line photochemical derivatisation and fluorescence detection.
The aflatoxins content of 140 cotton seed samples were determined using high-performance liquid chromatography. Samples were obtained from wholesalers in Iran between May 2010 and June 2011. Aflatoxin B₁ gave the highest incidence of contamination and was found in 129 of the 139 samples. The highest concentration of aflatoxin was 14.4 ng g⁻¹. Thirteen cotton seed samples (9.35%) were above one of the regulatory limits of the European Union (5 ng g⁻¹), but no sample was above the highest EU limit and the safety limit recommended by the FDA (20 ng g⁻¹) and regulatory limits of Iran (50 ng g⁻¹) for total aflatoxin.
a Testa Quality Control Laboratory , North-East Food Industrial Technology and Biotechnology Park , Mashhad , Iran.
Javad
J
Feizy
Hamed Reza
HR
Beheshti
Mohammad
M
Asadi
D016604:Aflatoxin B1 / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism; D000348:Aflatoxins / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D053000:Analytic Sample Preparation Methods; D000821:Animal Feed / Q000032:analysis / Q000191:economics / Q000382:microbiology; D001230:Aspergillus / Q000378:metabolism; D057205:Automation, Laboratory; D002138:Calibration; D002273:Carcinogens / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D018556:Crops, Agricultural / Q000737:chemistry* / Q000382:microbiology; D005506:Food Contamination; D005513:Food Inspection / Q000379:methods*; D003368:Gossypium / Q000737:chemistry* / Q000382:microbiology; D007492:Iran; D057230:Limit of Detection; D055668:Photochemical Processes; D012639:Seeds / Q000737:chemistry* / Q000382:microbiology; D013050:Spectrometry, Fluorescence
D000348:Aflatoxins; D002273:Carcinogens; C029753:aflatoxin B2; D016604:Aflatoxin B1
LC; aflatoxins; cotton seed; photochemical derivatisation