Adsorption and desorption of tyrosine kinase inhibitor erlotinib on gold nanoparticles.
We investigated interfacial behaviors of erlotinib (EL) on gold nanoparticles (AuNPs) by means of Raman spectroscopy. The adsorption reactions and structures of EL on AuNP surfaces were examined by UV-Vis absorption spectroscopy and surface-enhanced Raman scattering (SERS). Density functional theory calculations were performed to estimate the energetic stabilities of the drug-AuNP composites. Among the binding units in EL, the acetylenic C≡C group was calculated to be the most strongly binding on the AuNP cluster atoms, consistent with the SERS spectra. The concentration-dependent SERS spectra indicated that ∼10(-5) M of EL exhibited the highest SERS signals. The attached EL appeared to desorb more efficiently with 2mM glutathione than with cell culture media. The lack of a strong SERS signal of EL in the dark-field microscopy images of AuNP-EL complexes suggested almost complete desorption of EL inside cells.
Department of Chemistry, Soongsil University, Seoul 156-743, Republic of Korea.
Anh Thu Ngoc
AT
Lam
Department of Chemistry, Soongsil University, Seoul 156-743, Republic of Korea.
Jinha
J
Yoon
Department of Chemistry, Soongsil University, Seoul 156-743, Republic of Korea.
Erdene-Ochir
EO
Ganbold
Department of Physics, Sogang University, Seoul 121-742, Republic of Korea.
Dheeraj K
DK
Singh
Department of Physics, Sogang University, Seoul 121-742, Republic of Korea.
Doseok
D
Kim
School of Systems Biomedical Science, Soongsil University, Sangdo-dong, Dongjak-gu, Seoul, Republic of Korea.
Kwang-Hwi
KH
Cho
Department of Chemistry, Gachon University, Seongnam 461-701, Republic of Korea; Gachon Medical Research Institute, Gil Medical Center, Inchon 405-760, Republic of Korea.
Sang Jun
SJ
Son
Department of Bionano Technology, Hanyang University, Ansan 426-791, Republic of Korea.
Jaebum
J
Choo
College of Veterinary Medicine, Seoul National University, Seoul 156-743, Republic of Korea.
So Yeong
SY
Lee
Molecular-level Interface Research Center and Department of Chemistry, KAIST, Daejeon 305-701, Republic of Korea.
Sehun
S
Kim
Department of Chemistry, Soongsil University, Seoul 156-743, Republic of Korea. Electronic address: sjoo@ssu.ac.kr.
Sang-Woo
SW
Joo
D000327:Adsorption; D000069347:Erlotinib Hydrochloride; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles; D047428:Protein Kinase Inhibitors / Q000737:chemistry*; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors*; D011799:Quinazolines / Q000737:chemistry*; D013059:Spectrum Analysis, Raman
D047428:Protein Kinase Inhibitors; D011799:Quinazolines; D006046:Gold; D000069347:Erlotinib Hydrochloride; D011505:Protein-Tyrosine Kinases
Density functional theory; Erlotinib; Gold nanoparticles; Interfacial structures; Raman spectroscopy