Screening of the residual normal ovarian tissue adjacent to orthotopic epithelial ovarian carcinomas in nude mice.
The objective of this study was to explore the feasibility and methods of screening the residual normal ovarian tissue adjacent to orthotopic ovarian carcinomas in nude mice. Human epithelial ovarian cancer cells (OVCAR3) were subcutaneously implanted for a tumor source and ovarian orthotopic transplantation. The cancer tissue, proximal paraneoplastic tissue, middle paraneoplastic tissue, remote paraneoplastic tissue, and normal ovarian tissue were removed. CK-7, CA125, p53, survivin, MMP-2, and TIMP-2 expression was detected by reverse transcription polymerase chain reaction. We obtained 35 paraneoplastic residual ovarian tissues with normal biopsies from 40 cases of an orthotopic epithelial ovarian carcinoma model (87.5%). CK-7, CA125, p53, survivin, MMP-2, and TIMP-2 expression was lower in proximal paraneoplastic tissue than in cancer tissue (P < 0.05) and higher than in middle and remote paraneoplastic tissue (P < 0.01). There was no statistically significant difference between the expression of these genes in middle and proximal paraneoplastic tissue as well as among residual normal ovarian tissues with different severity (P > 0.05). In ovarian tissues of 20 normal nude mice, the expression of CK- 7, CA125, p53, survivin, MMP-2, and TIMP-2 was negative. Overall, the expression levels of CK-7, CA125, p53, survivin, MMP-2, TIMP-2, and other molecular markers showed a decreasing trend in the non-cancer tissue direction. The expression levels can be used as standards to screen residual normal ovarian tissue. We can obtain relatively safe normal ovarian tissues adjacent to epithelial ovarian cancer.
Department of Gynecology, Hainan Provincial People's Hospital, Haikou, Hainan, China genhaizhu@163.com.
G H
GH
Zhu
Department of Gynecology, Hainan Provincial People's Hospital, Haikou, Hainan, China.
S T
ST
Wang
Department of Gynecology, Hainan Provincial People's Hospital, Haikou, Hainan, China.
M Z
MZ
Yao
Research Center, Hainan Provincial People's Hospital, Haikou, Hainan, China.
J H
JH
Cai
Department of Gynecology, Hainan Provincial People's Hospital, Haikou, Hainan, China.
C Y
CY
Chen
Hainan Medical College, Drug Safety Evaluation Center, Haikou, Hainan, China.
Z X
ZX
Yang
Department of Gynecology, Hainan Provincial People's Hospital, Haikou, Hainan, China.
L
L
Hong
Department of Gynecology, Hainan Provincial People's Hospital, Haikou, Hainan, China.
S Y
SY
Yang
D000818:Animals; D001706:Biopsy; D000077216:Carcinoma, Ovarian Epithelial; D045744:Cell Line, Tumor; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051379:Mice; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D009375:Neoplasms, Glandular and Epithelial / Q000235:genetics* / Q000473:pathology; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000473:pathology; D023041:Xenograft Model Antitumor Assays
D009363:Neoplasm Proteins