Histologic and micro-computed tomographic analyses of replanted teeth stored in different kind of media.
BACKGROUND
Coconut water (CW) and soy milk (SM) have been proposed as storage media for avulsed teeth because of their nutrients that preserve cell viability. The present study investigated the periodontal healing process of dog teeth replanted after storage in CW, SM, and whole milk (WM) using micro-computed tomographic (μCT) and histologic analyses compared with immediate tooth replantation.


METHODS
Forty roots of 10 adult beagle dogs were extracted and subjected to the following protocols: immediate replantation after extraction (control), stored in CW with an adjusted pH, and SM and WM for 50 minutes before replantation. The animals were euthanized 28 days postoperatively, and the obtained specimens were scanned using a μCT scanner and subjected to routine processing for histometric analyses under an optical microscope.


RESULTS
CW and SM performed similarly to WM; however, SM showed significantly higher ankylosis than the control group.


CONCLUSIONS
Additionally, this study showed that the combined use of histologic analysis and μCT is a promising method to better identify tooth resorption and the repair process and to evaluate the total extension of the periodontium. CW as a storage medium is a promising transport media for avulsed teeth.
Biomechanics Group, Department of Operative Dentistry and Dental Materials, School of Dentistry, Federal University of Uberlândia, Uberlândia, Brazil.
Manuella Verdinelli
MV
de Paula Reis
Biomechanics Group, Department of Operative Dentistry and Dental Materials, School of Dentistry, Federal University of Uberlândia, Uberlândia, Brazil.
Camilla Christian Gomes
CC
Moura
Biomechanics Group, Department of Operative Dentistry and Dental Materials, School of Dentistry, Federal University of Uberlândia, Uberlândia, Brazil.
Priscilla Barbosa Ferreira
PB
Soares
Oral and Maxillofacial Surgery and Implantology Department, School of Dentistry, Federal University of Uberlândia, Uberlândia, Brazil.
Graziela Bianchi
GB
Leoni
Oral and Maxillofacial Surgery and Implantology Department, School of Dentistry, Federal University of Uberlândia, Uberlândia, Brazil.
Manoel Damião
MD
Souza-Neto
Department of Restorative Dentistry, Faculty of Dentistry, University of São Paulo, Ribeirão Preto, Brazil.
Darceny Zanetta
DZ
Barbosa
Biomechanics Group, Department of Operative Dentistry and Dental Materials, School of Dentistry, Federal University of Uberlândia, Uberlândia, Brazil. Electronic address: carlosjsoares@umuarama.ufu.br.
Carlos José
CJ
Soares
D000818:Animals; D003059:Cocos; D003739:Dental Cementum / Q000473:pathology; D004285:Dogs; D006863:Hydrogen-Ion Concentration; D008297:Male; D008892:Milk; D019188:Organ Preservation Solutions / Q000627:therapeutic use*; D010513:Periodontal Ligament / Q000473:pathology; D012391:Root Resorption / Q000473:pathology; D041942:Soy Milk; D013997:Time Factors; D014070:Tooth / Q000473:pathology*; D020254:Tooth Ankylosis / Q000473:pathology; D014084:Tooth Avulsion / Q000473:pathology / Q000628:therapy*; D014090:Tooth Replantation / Q000379:methods*; D014945:Wound Healing / Q000502:physiology; D055114:X-Ray Microtomography / Q000379:methods
D019188:Organ Preservation Solutions
Avulsed tooth; histologic analysis; micro–computed tomographic analysis; replantation; storage media