Analysis of diterpenes and triterpenes from plant foliage and roots.
Terpene specialized metabolites exhibit multiple functions in plant-environment interactions and plant development. Molecular biologists investigating the biochemistry and molecular function of terpenes need to apply robust but yet sensitive analytical methods optimized and adapted to the structural diversity and often varying concentrations of terpene compounds in plant tissues. Here we present hands-on protocols for sample preparation and GC-MS or LC-MS/MS analysis of selected diterpene and triterpene hydrocarbons or oxygenated derivatives from roots and shoots of Arabidopsis and rice.
Department of Biological Sciences, Virginia Tech, 220 Ag Quad Lane, Blacksburg, VA, 24061, USA.
Qiang
Q
Wang
Reza
R
Sohrabi
Dorothea
D
Tholl
D017360:Arabidopsis / Q000737:chemistry*; D004224:Diterpenes / Q000032:analysis* / Q000302:isolation & purification; D008401:Gas Chromatography-Mass Spectrometry; D012275:Oryza / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry*; D052617:Solid Phase Microextraction; D014315:Triterpenes / Q000032:analysis* / Q000302:isolation & purification
D004224:Diterpenes; D014315:Triterpenes