Influence of DMF-induced oxidative stress on membrane and periplasmic proteins in Paracoccus sp. SKG.
The present study describes the N,N-dimethylformamide (DMF)-induced oxidative stress in Paracoccus sp. SKG. The oxidative stress was evaluated by analysing membrane and periplasmic proteins and K+ efflux, as well as by monitoring the activities of antioxidant enzymes like catalase, superoxide dismutase (SOD) and glutathione S-transferase (GST). The exposure of bacterial cells to a higher concentration of DMF resulted in the modification of membrane fatty acid composition which is accompanied by K+ efflux. Further, this oxidative stress resulted in increased periplasmic protein which can be attributed to the induction of GST and methionine sulphoxide reductase (Msr) enzymes under solvent stress. Paracoccus sp. SKG is tolerant to high concentrations of DMF up to 6% (v/v) and its toxic effects. DMF concentration-dependent induction of GST and Msr activities advocates the significant role of these enzymes in the bacterial defence system. The present study provides information which helps us to understand the ROS scavenging machinery in bacteria. The high tolerance of Paracoccus sp. SKG to DMF can be efficiently explored for various bioremediation and biotransformation applications.
Department of Biochemistry, Gulbarga University, Gulbarga, 585106, Karnataka, India.
B
B
Kirankumar
Guruprasad B
GB
Kulkarni
S
S
Sanjeevkumar
I
I
Mukram
T B
TB
Karegoudar
D000975:Antioxidants / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects* / Q000378:metabolism*; D004126:Dimethylformamide / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D004790:Enzyme Induction / Q000187:drug effects; D005227:Fatty Acids / Q000378:metabolism; D005982:Glutathione Transferase / Q000096:biosynthesis; D051150:Methionine Sulfoxide Reductases / Q000096:biosynthesis; D018384:Oxidative Stress / Q000187:drug effects*; D010230:Paracoccus / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D033903:Periplasmic Proteins / Q000378:metabolism*; D011188:Potassium / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism
D000975:Antioxidants; D005227:Fatty Acids; D033903:Periplasmic Proteins; D004126:Dimethylformamide; D002374:Catalase; D013482:Superoxide Dismutase; D051150:Methionine Sulfoxide Reductases; C027219:methionine sulfoxide reductase; D005982:Glutathione Transferase; D011188:Potassium