Effect of immobilized hyaluronidase on stem and progenitor cells in pulmonary fibrosis.
The effect of immobilized hyaluronidase on stem and progenitor cells of the lungs was studied on the model of partially reversible toxic bleomycin-induced pulmonary fibrosis in C57Bl/6 mice. During the inflammation phase, immobilized hyaluronidase reduced infiltration of alveolar interstitium with hemopoietic stem cells Sca-1(+), c-Kit(+), CD34(-), (CD3, CD45R (B220), Ly6C, Ly6G (Gr1), CD11b (Mac1), TER-119)(-). Improvement of histological parameters of bleomycin lungs during the phase of collagen fiber deposition after the treatment was accompanied by accumulation of mesenchymal multipotent stromal cells (CD31(-), CD34(-), CD45(-), CD44(+), CD73(+), CD90(+), CD106(+)decrease in the population of pan-hemopoietic cells (CD45(+)), accelerated restoration of the content of endothelial cells, and inhibition of clonal activity of fibroblast precursors (CD45(-)).
Research Institute of Pharmacology, Siberian Division of the Russian Academy of Medical Sciences, Tomsk, Russia.
A M
AM
Dygai
E G
EG
Skurikhin
E S
ES
Khmelevskaya
N N
NN
Ermakova
A M
AM
Reztsova
O V
OV
Pershina
V A
VA
Krupin
I E
IE
Stepanova
V M
VM
Reztsova
A V
AV
Artamonov
A A
AA
Bekarev
P G
PG
Madonov
D N
DN
Kinsht
D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D001853:Bone Marrow / Q000276:immunology / Q000473:pathology; D004800:Enzymes, Immobilized / Q000008:administration & dosage*; D006821:Hyaluronoglucosaminidase / Q000008:administration & dosage*; D008168:Lung / Q000473:pathology; D008810:Mice, Inbred C57BL; D011658:Pulmonary Fibrosis / Q000188:drug therapy / Q000276:immunology / Q000473:pathology*; D013234:Stem Cells / Q000187:drug effects / Q000378:metabolism*
D015703:Antigens, CD; D004800:Enzymes, Immobilized; D006821:Hyaluronoglucosaminidase