[Identification of bufadienolides profiling in cinobufacino by HPLC-DAD-FT-ICR-MS method].
Cinobufacino injection is a significant anti-tumor medicine for the treatment of various tumors in clinic, which was made from water extraction of the skin of Bufo bufo gargarizans. In present paper, HPLC-DAD-FT-ICR-MS method was used to identify the major bufadienolides in cinobufacino for the first time. Solid-phase extraction with dichloromethane and silica was used to enrich the total bufadienolides in cinobufacino. Based on the UV and high resolution MS/MS data, 33 bufadienolides were analyzed and characterized. Among them, eight compounds were identified by comparing with standard references unambiguously. This study elucidated the major bufadienolides in cinobufacino, which provided material foundation of cinobufacino and will be benefit for the further pharmacological research.
Jun-Qiu
JQ
Liu
Nan
N
Si
Jian
J
Yang
Hai-Yu
HY
Zhao
Bao-Lin
BL
Bian
Hong-Jie
HJ
Wang
D000664:Amphibian Venoms / Q000737:chemistry*; D000818:Animals; D002018:Bufanolides / Q000032:analysis* / Q000737:chemistry; D002023:Bufo bufo; D002851:Chromatography, High Pressure Liquid; D015394:Molecular Structure; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
D000664:Amphibian Venoms; D002018:Bufanolides; C545813:huachansu; C005734:bufogenin